FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Yao, YJ Liang, SL Li, XL Liu, SM Chen, JQ Zhang, XT Jia, K Jiang, B Xie, XH Munier, S Liu, M Yu, J Lindroth, A Varlagin, A Raschi, A Noormets, A Pio, C Wohlfahrt, G Sun, G Domec, JC Montagnani, L Lund, M Eddy, M Blanken, PD Grunwald, T Wolf, S Magliulo, V AF Yao, Yunjun Liang, Shunlin Li, Xianglan Liu, Shaomin Chen, Jiquan Zhang, Xiaotong Jia, Kun Jiang, Bo Xie, Xianhong Munier, Simon Liu, Meng Yu, Jian Lindroth, Anders Varlagin, Andrej Raschi, Antonio Noormets, Asko Pio, Casimiro Wohlfahrt, Georg Sun, Ge Domec, Jean-Christophe Montagnani, Leonardo Lund, Magnus Eddy, Moors Blanken, Peter D. Gruenwald, Thomas Wolf, Sebastian Magliulo, Vincenzo TI Assessment and simulation of global terrestrial latent heat flux by synthesis of CMIP5 climate models and surface eddy covariance observations SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Global terrestrial LE; CMIP5; GCMs; BMA; Taylor skill score ID GENERAL-CIRCULATION MODELS; ENERGY-BALANCE CLOSURE; BAYESIAN MULTIMODEL ESTIMATION; DOWNWARD LONGWAVE RADIATION; CARBON-DIOXIDE; LAND; EVAPOTRANSPIRATION; PRECIPITATION; CHINA; ALGORITHM AB The latent heat flux (LE) between the terrestrial biosphere and atmosphere is a major driver of the global hydrological cycle. In this study, we evaluated LE simulations by 45 general circulation models (GCMs) in the Coupled Model Intercomparison Project Phase 5 (CMIP5) by a comparison with eddy covariance (EC) observations from 240 globally distributed sites from 2000 to 2009. In addition, we improved global terrestrial LE estimates for different land cover types by synthesis of seven best CMIP5 models and EC observations based on a Bayesian model averaging (BMA) method. The comparison results showed substantial differences in monthly LE among all GCMs. The model CESM1-CAM5 has the best performance with the highest predictive skill and a Taylor skill score (S) from 0.51-0.75 for different land cover types. The cross-validation results illustrate that the BMA method has improved the accuracy of the CMIP5 GCM's LE simulation with a decrease in the averaged root-mean-square error (RMSE) by more than 3 W/m(2) when compared to the simple model averaging (SMA) method and individual GCMs. We found an increasing trend in the BMA-based global terrestrial LE (slope of 0.018 W/m(2) yr(-1), p < 0.05) during the period 1970-2005. This variation may be attributed directly to the inter-annual variations in air temperature (T-a), surface incident solar radiation (R-s) and precipitation (P). However, our study highlights a large difference from previous studies in a continuous increasing trend after 1998, which may be caused by the combined effects of the variations of R-s, T-a, and P on LE for different models on these time scales. This study provides corrected-modeling evidence for an accelerated global water cycle with climate change. (C) 2016 Elsevier B.V. All rights reserved. C1 [Yao, Yunjun; Liang, Shunlin; Liu, Shaomin; Zhang, Xiaotong; Jia, Kun; Jiang, Bo; Xie, Xianhong; Liu, Meng; Yu, Jian] Beijing Normal Univ, Sch Geog, State Key Lab Remote Sensing Sci, Beijing 100875, Peoples R China. [Li, Xianglan] Beijing Normal Univ, Coll Global Change & Earth Syst Sci, Beijing 100875, Peoples R China. [Chen, Jiquan] Michigan State Univ, CGCEO Geog, E Lansing, MI 48824 USA. [Munier, Simon] LEGOS CNES CN RS IRD UPS UMR5566, Lab Detudes & Phys & Oceanographie Spatiles, Toulouse, France. [Lindroth, Anders] Lund Univ, GeoBiosphere Sci Ctr, S-22362 Lund, Sweden. [Varlagin, Andrej] ANSevertsov Inst Ecol & Evolut RAS 123103, Moscow, Russia. [Raschi, Antonio] CNR, CNR IBIMET, I-50145 Florence, Italy. [Noormets, Asko] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Pio, Casimiro] Univ Aveiro, CESAM & Dept Ambiente Ordenamento, P-3810193 Aveiro, Portugal. [Wohlfahrt, Georg] Univ Innsbruck, Inst Ecol, A-6020 Innsbruck, Austria. [Wohlfahrt, Georg] European Acad Bolzano, I-39100 Bolzano, Italy. [Sun, Ge] United States Dept Agr Forest Serv, Southern Res Stn, Eastern Forest Environm Threat Assessment Ctr, Raleigh, NC 27606 USA. [Domec, Jean-Christophe] UMR INRA ISPA 1391, Bordeaux Sci Agro, F-33195 Gradignan, France. [Domec, Jean-Christophe] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Montagnani, Leonardo] Free Univ Bolzano, Fac Sci & Technol, I-39100 Bolzano, Italy. [Montagnani, Leonardo] Autonomous Prov Bolzano, Forest Serv, I-39100 Bolzano, Italy. [Lund, Magnus] Aarhus Univ, Dept Scibiol, DK-4000 Roskilde, Denmark. [Eddy, Moors] Alterra Wageningen UR, Climate Change & Adapt Land & Water Management, NL-6700 AA Wageningen, Netherlands. [Eddy, Moors] Vrije Univ Amsterdam, NL-1081 HV Amsterdam, Netherlands. [Blanken, Peter D.] Univ Colorado, Dept Geog, Boulder, CO 80309 USA. [Gruenwald, Thomas] Tech Univ Dresden, Inst Hydrol & Meteorol, Chair Meteorol, D-01062 Dresden, Germany. [Wolf, Sebastian] Swiss Fed Inst Technol, Inst Agr Sci, CH-8092 Zurich, Switzerland. [Magliulo, Vincenzo] CNR ISAFOM, Natl Res Council, I-80040 Naples, Italy. RP Yao, YJ (reprint author), Beijing Normal Univ, Sch Geog, State Key Lab Remote Sensing Sci, Beijing 100875, Peoples R China. EM boyyunjun@163.com RI Wolf, Sebastian/B-4580-2010; Montagnani, Leonardo/F-1837-2016; CESAM, UA/M-3762-2015; Pio, Casimiro/A-8135-2008; Lund, Magnus/J-4922-2013; Wohlfahrt, Georg/D-2409-2009; liang, shunlin/C-2809-2015; Chen, Jiquan/D-1955-2009; OI Wolf, Sebastian/0000-0001-7717-6993; Montagnani, Leonardo/0000-0003-2957-9071; Pio, Casimiro/0000-0002-3531-8620; Lund, Magnus/0000-0003-1622-2305; Wohlfahrt, Georg/0000-0003-3080-6702; Lindroth, Anders/0000-0002-7669-784X FU U.S. Department of Energy, Biological and Environmental Research, Terrestrial Carbon Program [DE-FG02-04ER63917, DE-FG02-04ER63911]; CarboEuropeIP; FAO-GTOS-TCO; iLEAPS; Max Planck Institute for Biogeochemistry; National Science Foundation; University of Tuscia; University Laval; Environment Canada; US Department of Energy; Natural Science Fund of China [41201331, 41331173, 41205104]; High-Tech Research and Development Program of China [2013AA122801]; European Commission FX The authors thank the anonymous reviewers for their critical and helpful comments and suggestions. CMIP5 GCM LE simulation was downloaded from online (http://cmip-pcmdi.llnl.gov/cmip5/data_portal.html). Monthly GEWEX Rs product was obtained from online (http://gewex-srb.larc.nasa.gov/common/php/SRBdataproducts.php). Monthly ERA- Interim Ta dataset was downloaded from online (http://www.ecmwf.int/research/era/do/get/era-interim) and monthly PGF P product was obtained from online (http://hydrology.princeton.edu/data/pgf/1.0deg/monthly/). This work also used eddy covariance data acquired by the FLUXNET community and in particular by the following networks: AmeriFlux (U.S. Department of Energy, Biological and Environmental Research, Terrestrial Carbon Program (DE-FG02-04ER63917 and DE-FG02-04ER63911)), AfriFlux, AsiaFlux, CarboAfrica, CarboEuropelP, CarboItaly, CarboMont,ChinaFlux, Fluxnet-Canada (supported by CFCAS, NSERC, BIOCAP, Environment Canada, and NRCan), GreenGrass, KoFlux, LBA, NECC, OzFlux, TCOS-Siberia, USCCC. We acknowledge the financial support to the eddy covariance data harmonization provided by CarboEuropeIP, FAO-GTOS-TCO, iLEAPS, Max Planck Institute for Biogeochemistry, National Science Foundation, University of Tuscia, University Laval, Environment Canada and US Department of Energy and the database development and technical support from Berkeley Water Center, Lawrence Berkeley National Laboratory, Microsoft Research eScience, Oak Ridge National Laboratory, University of California-Berkeley and the University of Virginia. Other ground-measured data were obtained from the Coordinated Enhanced Observation Project (CEOP) in arid and semi-arid regions of northern China (http://observation.tea.ac.cn/). This work was partially supported by the Natural Science Fund of China (No. 41201331, No. 41331173 and No. 41205104), and the High-Tech Research and Development Program of China (No.2013AA122801). S. Wolf acknowledges support from a Marie Curie International Outgoing Fellowship (European Commission). NR 87 TC 2 Z9 3 U1 16 U2 37 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD JUN 15 PY 2016 VL 223 BP 151 EP 167 DI 10.1016/j.agrformet.2016.03.016 PG 17 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA DN1OJ UT WOS:000376835000014 ER PT J AU Sang-Ngern, M Youn, UJ Park, EJ Kondratyuk, TP Simmons, CJ Wall, MM Ruf, M Lorch, SE Leong, E Pezzuto, JM Chang, LC AF Sang-ngern, Mayuramas Youn, Ui Joung Park, Eun-Jung Kondratyuk, Tamara P. Simmons, Charles J. Wall, Marisa M. Ruf, Michael Lorch, Sam E. Leong, Ethyn Pezzuto, John M. Chang, Leng Chee TI Withanolides derived from Physalis peruviana (Poha) with potential anti-inflammatory activity SO BIOORGANIC & MEDICINAL CHEMISTRY LETTERS LA English DT Article DE Physalis peruviana; Withanolides; X-ray crystallography; Anti-inflammatory activity ID WITHANIA-SOMNIFERA; SOLANACEAE; GROWTH; LEAVES; CELLS AB Three new withanolides, physaperuvin G (1), with physaperuvins I (2), and J (3), along with seven known derivatives (4-10), were isolated from the aerial parts of Physalis peruviana. The structures of 1-3 were determined by NMR, X-ray diffraction, and mass spectrometry. Compounds 1-10 were evaluated in lipopolysaccharide (LPS)-activated murine macrophage RAW 264.7 cells. Compounds 4, 5, and 10 with potent nitric oxide inhibitory activity in LPS-activated RAW 264.7 cells, with IC50 values in the range of 0.32-7.8 mu M. In addition, all compounds were evaluated for potential to inhibit tumor necrosis factor- alpha (TNF-alpha)-activated nuclear factor-kappa B (NF-kappa B) activity with transfected human embryonic kidney cells 293. Compounds 4-7 inhibited TNF-alpha-induced NF-kappa B activity with IC50 values in the range of 0.04-5.6 mu M. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Sang-ngern, Mayuramas; Youn, Ui Joung; Park, Eun-Jung; Kondratyuk, Tamara P.; Pezzuto, John M.; Chang, Leng Chee] Univ Hawaii, Daniel K Inouye Coll Pharm, Dept Pharmaceut Sci, Hilo, HI 96720 USA. [Simmons, Charles J.] Univ Hawaii, Dept Chem, Hilo, HI 96720 USA. [Wall, Marisa M.] USDA, Daniel K Inouye US Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. [Ruf, Michael] Bruker AXS Inc, East Cheryl Pkwy, Madison, WI 53711 USA. [Lorch, Sam E.] Lani Ko Honua Berry Farm, Pepeekeo, HI 96783 USA. [Leong, Ethyn] Cornell Univ, Coll Agr & Life Sci, Dept Food Sci, Founders Hall, Ithaca, NY 14853 USA. RP Chang, LC (reprint author), Univ Hawaii, Daniel K Inouye Coll Pharm, Dept Pharmaceut Sci, Hilo, HI 96720 USA. EM lengchee@hawaii.edu FU National Center for Research Resources [2G12RR003061-26]; National Institute on Minority Health and Health Disparities [8G12MD7601-27]; Royal Thai Government Program FX This research was supported by grant 2G12RR003061-26 from the National Center for Research Resources and grant 8G12MD7601-27 from the National Institute on Minority Health and Health Disparities (to L.C.C). M.S. thank a full scholarship from the Royal Thai Government Program and Dr. Antony Otsuka, of the DKICP, for his guidance and support. NR 27 TC 0 Z9 0 U1 7 U2 11 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0960-894X EI 1464-3405 J9 BIOORG MED CHEM LETT JI Bioorg. Med. Chem. Lett. PD JUN 15 PY 2016 VL 26 IS 12 BP 2755 EP 2759 DI 10.1016/j.bmcl.2016.04.077 PG 5 WC Chemistry, Medicinal; Chemistry, Organic SC Pharmacology & Pharmacy; Chemistry GA DN0BA UT WOS:000376728200002 PM 27210437 ER PT J AU Peterson, DW Dodson, EK AF Peterson, David W. Dodson, Erich Kyle TI Post-fire logging produces minimal persistent impacts on understory vegetation in northeastern Oregon, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Post-fire logging; Salvage logging; Understory vegetation; Species richness; Biodiversity; Post-fire forest management ID WESTERN UNITED-STATES; BOREAL MIXEDWOOD FOREST; PONDEROSA PINE; PLANT-COMMUNITIES; SPECIES COMPOSITION; FUEL ACCUMULATION; SALVAGE; WILDFIRE; FIRE; COVER AB Post-fire forest management commonly requires accepting some negative ecological impacts from management activities in order to achieve management objectives. Managers need to know, however, whether ecological impacts from post-fire management activities are transient or cause long-term ecosystem degradation. We studied the long-term response of understory vegetation to two post-fire logging treatments- commercial salvage logging with and without additional fuel reduction logging- on a long-term post-fire logging experiment in northeastern Oregon, USA. We sampled understory plant cover and species diversity on 10-11 sampling plots within each of nine experimental treatment units to see if post-fire logging treatments produced any lasting effects on understory plant cover, species diversity, community composition, or exotic species cover. Post-fire logging treatments produced no significant effects on understory vegetation cover, diversity, or community composition 15 years after treatment. We found no significant treatment effects on graminoid, forb, woody plant, or exotic plant cover and species richness, and differences among treatment means were generally small. Differences in treatment means were larger at the individual species level, but were only statistically significant for one native grass, California brome (Bromus carinatus), which responded differently to the two logging treatments. Multivariate analysis of understory plant communities across 91 sample plots found two major gradients in understory plant community composition, one correlated with regenerating forest (sapling) density and one correlated with residual overstory tree density, suggesting that initial fire severity (tree mortality) and post-fire regeneration may have greater long-term impacts on post-fire understory vegetation than post-fire logging. This study demonstrates that understory vegetation can be resilient to post-fire logging, particularly when best management practices, like logging over snow, are used to limit damage to soils and understory vegetation. Further research is needed to establish the generality of our results and to identify sources of variability in understory plant community responses to wildfire and post fire logging. If further research confirms our findings, post-fire logging debates will be able to focus more on how to mitigate short-term disturbance impacts and manage fire-killed trees to meet wildlife habitat, fuel reduction, and economic objectives, and less on concerns over long-term ecosystem degradation. Published by Elsevier B.V. C1 [Peterson, David W.] USDA Forest Serv, Pacific NW Res Stn, 1133 N Western Ave, Wenatchee, WA 98801 USA. [Dodson, Erich Kyle] Oregon State Univ, Dept Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA. [Dodson, Erich Kyle] USDA Forest Serv, Rocky Mt Res Stn, Interior West Forest Inventory & Anal, 507 25th St, Ogden, UT 84401 USA. RP Peterson, DW (reprint author), 1133 North Western Ave, Wenatchee, WA 98801 USA. EM davepeterson@fs.fed.us; erichdodson@fs.fed.us FU Malheur National Forest; Blue Mountains Natural Resources Institute (USDA Forest Service, PNW Research Station); U.S. Joint Fire Science Program [11-1-1-19]; USDA Forest Service; PNW Research Station; National Fire Plan FX We thank Jim McIver, Roger Ottmar, Bob Vihnanek, and the Malheur National Forest staff for their work designing, implementing, and maintaining the post-fire logging experiment. The Malheur National Forest, the Blue Mountains Natural Resources Institute (USDA Forest Service, PNW Research Station), and the U.S. Joint Fire Science Program (Project #11-1-1-19) funded earlier work on this experiment. We thank the USDA Forest Service, PNW Research Station, and the National Fire Plan for funding this phase of the study. We also thank Emily Orling, Robert J. Smith, Ashlee Mikkelsen, and Mari Schramm for their work on the botanical and stand structure surveys for this study. We thank Roger Ottmar and two anonymous reviewers for helpful suggestions that improved this paper. NR 59 TC 2 Z9 2 U1 12 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUN 15 PY 2016 VL 370 BP 56 EP 64 DI 10.1016/j.foreco.2016.04.004 PG 9 WC Forestry SC Forestry GA DK8GB UT WOS:000375164100006 ER PT J AU Yesilonis, I Szlavecz, K Pouyat, R Whigham, D Xia, L AF Yesilonis, I. Szlavecz, K. Pouyat, R. Whigham, D. Xia, L. TI Historical land use and stand age effects on forest soil properties in the Mid-Atlantic US SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Forest; Land-use change; Agriculture; Forest age; Nutrients ID NORTHEASTERN UNITED-STATES; ORGANIC-MATTER CHEMISTRY; EARTHWORM INVASION; HARDWOOD FOREST; PLANT-COMMUNITIES; CARBON; NITROGEN; IMPACT; VEGETATION; MARYLAND AB The conversion of agriculture lands to forest has been occurring in parts of North America for decades. The legacy of management activity during this transition is reflected in soil physical and chemical properties years after abandonment. This study was conducted at the Smithsonian Environmental Research Center, Maryland, USA, to determine land-use history and forest age effects on soil nutrients, carbon, pH, and bulk density. Soils in young and old successional forests and forests with no evidence of historical disturbance were sampled. The young forest stands were abandoned from agriculture 50-70 years ago and the old forest stands had been abandoned from agriculture or grazing 120-150 years ago. The oldest forest stands had no recorded history of disturbance even though it is likely they were at least disturbed by tree removal or grazing of animals in the colonial era. Young forest soils had higher concentrations of Mg, Ca, NO3 and a higher pH than old, which may be an age effect. The old forest soils that had been abandoned from agriculture and grazing had higher bulk density and lower C content than undisturbed stands indicating a land-use effect. In the stands that were formally agriculture there was evidence of erosion, indicated by a Bt horizon closer to the surface. The most evident difference between stands of different land:use history was the absence of a well-developed 0 horizon, which we attribute to the presence of earthworms. Land-use legacy set the forest ecosystem in a different trajectory of soil evolution. Published by Elsevier B.V. C1 [Yesilonis, I.] USDA Forest Serv, 5523 Res Pk,Suite 350, Baltimore, MD 21228 USA. [Szlavecz, K.; Xia, L.] Johns Hopkins Univ, Dept Earth & Planetary Sci, Olin Hall 226,3400 N Charles St, Baltimore, MD 21218 USA. [Pouyat, R.] USDA, Forest Serv Res & Dev, 1400 Independence Ave SW, Washington, DC 20250 USA. [Whigham, D.] Smithsonian Environm Res Ctr, 647 Contees Wharf Rd, Edgewater, MD 21037 USA. RP Yesilonis, I (reprint author), USDA Forest Serv, 5523 Res Pk,Suite 350, Baltimore, MD 21228 USA. EM iyesilonis@fs.fed.us OI Whigham, Dennis/0000-0003-1488-820X FU United States Department of Agriculture (USDA-CSREES) [NRI 2007-35320-18375]; National Science Foundation [EEC 0540832 ERC-MIRTHE] FX This study was partially supported by Grants from the United States Department of Agriculture (USDA-CSREES NRI 2007-35320-18375) and the National Science Foundation (EEC 0540832 ERC-MIRTHE). We thank Nancy Goff (SERC Nutrient Lab), Jay O'Neill (SERC Plant Ecology Lab), Emma Powell (USDA Forest Service), and Dean Cowherd (NRCS) for their help in various stages in the laboratory and in the field. NR 77 TC 2 Z9 2 U1 16 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUN 15 PY 2016 VL 370 BP 83 EP 92 DI 10.1016/j.foreco.2016.03.046 PG 10 WC Forestry SC Forestry GA DK8GB UT WOS:000375164100009 ER PT J AU Rasooly, R Do, PM Hernlem, BJ AF Rasooly, Reuven Do, Paula M. Hernlem, Bradley J. TI Low cost quantitative digital imaging as an alternative to qualitative in vivo bioassays for analysis of active aflatoxin B1 SO BIOSENSORS & BIOELECTRONICS LA English DT Article DE CCD camera; Fluorescence detector; Aflatoxin ID AFLATOXICOSIS; METABOLISM; PREVENTION; TOXICITY; OUTBREAK; FOOD; B-1 AB Aflatoxin B1 (AFB1) producing fungi contaminate food and feed and are a major health concern. To minimize the sources and incidence of AFB1 illness there is a need to develop affordable, sensitive mobile devices for detection of active AFB1. In the present study we used a low cost fluorescence detector and describe two quantitative assays for detection of detoxified and active AFB1 demonstrating that AFB1 concentration can be measured as intensity of fluorescence. When the assay plate containing increasing concentrations of AFB1 is illuminated with a 366 nm ultraviolet lamp, AFB1 molecules absorb photons and emit blue light with peak wavelength of 432 nm. The fluorescence intensity increased in dose dependent manner. However, this method cannot distinguish between active AFB1 which poses a threat to health, and the detoxified AFB1 which exhibits no toxicity. To measure the toxin activity, we used a cell based assay that makes quantification more robust and is capable of detecting multiple samples simultaneously. It is an alternative to the qualitative duckling bioassay which is the "gold-standard" assay currently being used for quantitative analysis of active AFB1. AFB1 was incubated with transduced Vero cells expressing the green fluorescence protein (GFP) gene. After excitation with blue light at 475 nm, cells emitted green light with emission peak at 509 nm. The result shows that AFB1 inhibits protein expression in a concentration dependent manner resulting in proportionately less GFP fluorescence in cells exposed to AFB1. The result also indicates strong positive linear relationship with R-2=0.90 between the low cost CCD camera and a fluorometer, which costs 100 times more than a CCD camera. This new analytical method for measuring active AFB1 is low in cost and combined with in vitro assay, is quantitative. It also does not require the use of animals and may be useful especially for laboratories in regions with limited resources. Published by Elsevier B.V. C1 [Rasooly, Reuven; Do, Paula M.; Hernlem, Bradley J.] ARS, Western Reg Res Ctr, Foodbome Contaminants Res Unit, USDA, Albany, CA USA. RP Rasooly, R (reprint author), ARS, Western Reg Res Ctr, Foodbome Contaminants Res Unit, USDA, Albany, CA USA. EM reuven.rasooly@ars.usda.gov NR 24 TC 1 Z9 1 U1 8 U2 38 PU ELSEVIER ADVANCED TECHNOLOGY PI OXFORD PA OXFORD FULFILLMENT CENTRE THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-5663 EI 1873-4235 J9 BIOSENS BIOELECTRON JI Biosens. Bioelectron. PD JUN 15 PY 2016 VL 80 BP 405 EP 410 DI 10.1016/j.bios.2016.01.087 PG 6 WC Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology SC Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics GA DH1PX UT WOS:000372558500058 PM 26874107 ER PT J AU Machtinger, ET Weeks, ENI Geden, CJ AF Machtinger, E. T. Weeks, E. N. I. Geden, C. J. TI Oviposition Deterrence and Immature Survival of Filth Flies (Diptera: Muscidae) When Exposed to Commercial Fungal Products SO JOURNAL OF INSECT SCIENCE LA English DT Article DE house fly; stable fly; Beauveria bassiana; Metarhizium anisopliae; Metarhizium brunneum; biological control ID MUSCA-DOMESTICA DIPTERA; ADULT HOUSE-FLY; BEAUVERIA-BASSIANA; COPTOTERMES-FORMOSANUS; ENTOMOPATHOGENIC FUNGI; METARHIZIUM-ANISOPLIAE; LABORATORY EVALUATION; STABLE FLIES; BEHAVIOR; FORMULATIONS AB Filth flies are pests of livestock, and can transmit pathogens that cause disease to animals and their caretakers. Studies have shown successful infection of adult filth flies following exposure to different strains and formulations of entomopathogenic fungi. This study aimed to examine the effects of commercial formulations of Beauveria bassiana (Balsamo) (Moniliales: Moniliaceae) (i.e., BotaniGard ES, Mycotrol O, balEnce), and Metarhizium brunneum (Metsch.) (Ascomycota: Hypocreales) (i.e., Met52 EC), on filth fly oviposition and immature fly survival after exposure. House flies, Musca domestica L., laid significantly fewer eggs on Met52 EC-treated surfaces than on surfaces treated with all other products and the control. Similar numbers of eggs were laid on surfaces treated with all B. bassiana products, but egg production was half of the control. Stable flies, Stomoxys calcitrans (L.), laid the fewest eggs on Met52 EC- and Mycotrol O-treated surfaces. This species did not distinguish between the remaining products and the control. In a second experiment, house fly eggs were placed on treated cloths so that hatched larvae contacted the treatment prior to development. Met52 EC had the greatest effect on immature survival with a significant reduction in recovered pupae at the medium and high doses of fungi. Overall, Met52 EC, containing M. brunneum, had the greatest effect on house fly and stable fly oviposition deterrence and immature development of house flies. Management implications are discussed. C1 [Machtinger, E. T.] USDA ARS, Invas Insect Biocontrol & Behav Lab, 10300 Baltimore Ave Beltsville, Beltsville, MD 20705 USA. [Weeks, E. N. I.] Univ Florida, Dept Entomol & Nematol, POB 110620, Gainesville, FL 32611 USA. [Geden, C. J.] USDA ARS, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. RP Geden, CJ (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. EM chris.geden@ars.usda.gov FU University of Florida Agricultural Experiment Station Federal Formula Funds (Cooperative State Research, Education and Extension Service, US Department of Agriculture) [FLA-ENY-005351] FX The authors would like to thank Dana Pippin for her assistance in house fly rearing, and Dr. Phillip Kaufman and Dr. Jerry Hogsette for providing the house flies and stable flies, respectively, used in this research. Funding was provided by the University of Florida Agricultural Experiment Station Federal Formula Funds, project FLA-ENY-005351 (Cooperative State Research, Education and Extension Service, US Department of Agriculture). NR 38 TC 1 Z9 1 U1 6 U2 6 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1536-2442 EI 2250-2645 J9 J INSECT SCI JI J Insect Sci. PD JUN 14 PY 2016 VL 16 AR 54 DI 10.1093/jisesa/iew032 PG 6 WC Entomology SC Entomology GA DQ0DS UT WOS:000378869200001 ER PT J AU Todoroki, CL Lowell, EC AF Todoroki, Christine L. Lowell, Eini C. TI Validation of models predicting modulus of elasticity in Douglas-fir trees, boles, and logs SO NEW ZEALAND JOURNAL OF FORESTRY SCIENCE LA English DT Article DE Accuracy; Density; Modulus of elasticity; Acoustic velocity; Douglas-fir; Mixed-effects models ID ACOUSTIC MEASUREMENTS; WOOD STIFFNESS; DENSITY; STANDS; SEGREGATION; LUMBER AB Background: Acoustic tools have simplified estimation of wood modulus of elasticity (MOE). Strong relationships between acoustic velocity and MOE of logs have encouraged use of acoustics at earlier points in the value chain, culminating in the development of acoustic harvesting systems. With accurate estimates of MOE of individual trees, improvements in efficiency along the value chain and increased value recovery will result. Our aim was to quantify the accuracy of MOE estimates at three distinct points: pre-harvest (standing trees), during harvest (merchantable boles), and post-harvest (5-m logs). We hypothesised that: (1) MOE estimated from acoustic velocity and wood density would provide greatest accuracy; and (2) bole estimates with a resonance tool would be more accurate than tree estimates with a time-of-flight tool. Methods: A sample of 168 Douglas-fir (Pseudotsuga menziesii [Mirb. Franco]) trees, representing the variability in acoustic velocity of 700 standing 36-51-year-old trees, was harvested from three sites. Prior to harvest, time-of-flight and breast-height diameter were recorded. After felling, resonance velocities of boles and subsequent 5-m logs were recorded. Discs, cut from log ends, were immersed, and green wood density determined. Half the logs were processed into boards, the other half into veneer sheets, and all products (in excess of 6000) non-destructively tested for MOE. MOE of parent trees, boles, and logs was then calculated from the mean MOE of derived products. Predictive mixed-effects models of tree, bole, and log MOE were developed using data from 139 trees. Fixed effects comprised combinations of velocity squared, wood density, acoustic MOE (derived from the wave equation), diameter, height, taper, and age. Random effects comprised site, plot, and, at the log level, tree. The models were validated using data from the remaining trees and compared using multiple performance metrics. Results: For estimating tree MOE, a model with velocity squared, wood density, and taper as predictors is recommended. For estimating MOE of boles and logs, models with velocity squared and wood density are recommended. The models have an accuracy, as determined by RMSE, of about +/- 2 GPa. Conclusions: For accurate MOE estimation, velocity alone is insufficient. Knowledge of wood density is necessary for improved accuracy. C1 [Todoroki, Christine L.] Scion, Private Bag 3020, Rotorua 3046, New Zealand. [Lowell, Eini C.] USDA Forest Serv, Portland Forestry Sci Lab, 620 SW Main,Suite 400, Portland, OR 97205 USA. RP Todoroki, CL (reprint author), Scion, Private Bag 3020, Rotorua 3046, New Zealand. EM Christine.Todoroki@scionresearch.com FU New Zealand Forest Research Institute Limited, trading as Scion; USDA Forest Service; Sustainable Forest Productivity component of Agenda; USDA Forest Service Research Development; American Forest and Paper Association FX This research effort was supported by a Joint Venture Agreement between the New Zealand Forest Research Institute Limited, trading as Scion, and the USDA Forest Service. Support was also provided by the Sustainable Forest Productivity component of Agenda 2020, a joint effort of the USDA Forest Service Research & Development and the American Forest and Paper Association. We are grateful and wish to express our gratitude to the companies and individuals who contributed to the processing and collection of data underpinning this study. We also wish to thank the reviewers of this manuscript for their insightful comments and suggestions. NR 47 TC 0 Z9 0 U1 3 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0048-0134 EI 1179-5395 J9 NZ J FORESTRY SCI JI N. Z. J. Forest. Sci. PD JUN 14 PY 2016 VL 46 AR 11 DI 10.1186/s40490-016-0067-x PG 15 WC Forestry SC Forestry GA DQ2OU UT WOS:000379042900001 ER PT J AU Savaris, M Marinoni, L Norrbom, AL AF Savaris, Marcoandre Marinoni, Luciane Norrbom, Allen L. TI FAMILY TEPHRITIDAE SO ZOOTAXA LA English DT Article DE catalogue; Colombia; distribution; taxonomy; Tephritidae ID ANASTREPHA DIPTERA TEPHRITIDAE; SPECIES GROUP DIPTERA; GENUS ANASTREPHA; SCHINER DIPTERA; FRUIT-FLIES; COLOMBIA; REVISION; KEY; RECORDS; BRAZIL AB The present Catalogue includes 90 species and 23 genera of Tephritidae that have been recorded in Colombia. Four subfamilies (Blepharoneurinae, Dacinae, Trypetinae and Tephritinae), and eight tribes (Acrotaeniini, Carpomyini, Dacini, Eutretini, Myopitini, Noeetini, Tephritini, and Toxotrypanini) are represented in the Colombian territory. The species Toxotrypana littoralis and Tomoplagia pleuralis are new records for the country. Trypeta conferta Walker, 1853 is here formally transferred to the genus Neomyopites. C1 [Savaris, Marcoandre; Marinoni, Luciane] Univ Fed Parana, Dept Zool, Ctr Politecn, Caixa Postal 19020, BR-81531980 Curitiba, Parana, Brazil. [Norrbom, Allen L.] ARS, Systemat Entomol Lab, USDA, Smithsonian Inst, POB 37012,MRC 168, Washington, DC 20013 USA. RP Savaris, M (reprint author), Univ Fed Parana, Dept Zool, Ctr Politecn, Caixa Postal 19020, BR-81531980 Curitiba, Parana, Brazil. EM plaumannimyia@gmail.com; lmarinoni@ufpr.br; allen.norrbom@ars.usda.gov RI Gesseff, Ednilson/A-3019-2017; Marinoni, Luciane /C-5720-2013 FU CNPq [200277/2015-4] FX We thank the editors, Marta Wolff and Claudio J. B. Carvalho, for inviting us to complete the fruit fly component of this catalog. We also thank Silvio S. Nihei for reviewing the manuscript. USDA is an equal opportunity provider and employer. We thank CNPq for financial support for this study (Process number 200277/2015-4). NR 189 TC 0 Z9 0 U1 4 U2 4 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD JUN 14 PY 2016 VL 4122 IS 1 BP 596 EP 621 DI 10.11646/zootaxa.4122.1.50 PG 26 WC Zoology SC Zoology GA DO4NO UT WOS:000377760000049 PM 27395298 ER PT J AU Chien, SY Sheen, S Sommers, CH Sheen, LY AF Chien, Shih-Yung Sheen, Shiowshuh Sommers, Christopher H. Sheen, Lee-Yan TI Modeling the Inactivation of Intestinal Pathogenic Escherichia coli 0157:H7 and Uropathogenic E. coli in Ground Chicken by High Pressure Processing and Thymol SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE modeling; high pressure processing; thymol; E. coif 0157:H7; uropathogenic E. coli; ground chicken ID ESSENTIAL OILS; FOODBORNE ILLNESS; LISTERIA-MONOCYTOGENES; FOOD; SALMONELLA; ANTIBACTERIAL; BEEF; MECHANISMS; HUMANS; IMPACT AB Disease causing Escherichia coil commonly found in meat and poultry include intestinal pathogenic E. coil (iPEC) as well as extraintestinal types such as the Uropathogenic E coli (UPEC). In this study we compared the resistance of iPEC (0157:H7) to UPEC in chicken meat using High Pressure Processing (HPP) in with (the hurdle concept) and without thymol essential oil as a sensitizer. UPEC was found slightly more resistant than E coli 0157:H7 OPEC 0157:H7) at 450 and 500 MPa. A central composite experimental design was used to evaluate the effect of pressure (300-400 MPa), thymol concentration (100-200 ppm), and pressure-holding time (10-20 min) on the inactivation of iPEC 0157:H7 and UPEC in ground chicken. The hurdle approach reduced the high pressure levels and thymol doses imposed on the food matrices and potentially decreased food quality damaged after treatment. The quadratic equations were developed to predict the impact (lethality) on iPEC 0157:H7 (R-2 = 0.94) and UPEC (R-2 = 0.98), as well as dimensionless non-linear models [Pr > F (<0.0001)]. Both linear and non-linear models were validated with data obtained from separated experiment points. All models may predict the inactivation/lethality within the same order of accuracy. However, the dimensionless non-linear models showed potential applications with parameters outside the central composite design ranges. The results provide useful information of both iPEC 0157:H7 and UPEC in regard to how they may survive HPP in the presence or absence of thymol. The models may further assist regulatory agencies and food industry to assess the potential risk of iPEC 0157:H7 and UPEC in ground chicken. C1 [Chien, Shih-Yung; Sheen, Lee-Yan] Natl Taiwan Univ, Inst Food Sci & Technol, Taipei 10764, Taiwan. [Chien, Shih-Yung; Sheen, Shiowshuh; Sommers, Christopher H.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA USA. RP Sheen, S (reprint author), ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA USA. EM shiowshuh.sheen@ars.usda.gov FU USDA-ARS National Program 108 Food Safety Project [8072-42000-073-00D] FX This Project was funded by USDA-ARS National Program 108 Food Safety Project: No. 8072-42000-073-00D. NR 45 TC 1 Z9 1 U1 6 U2 7 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD JUN 14 PY 2016 VL 7 AR 920 DI 10.3389/fmicb.2016.00920 PG 11 WC Microbiology SC Microbiology GA DO3CF UT WOS:000377657000001 PM 27379050 ER PT J AU Faburay, B Wilson, WC Gaudreault, NN Davis, AS Shivanna, V Bawa, B Sunwoo, SY Ma, WJ Drolet, BS Morozov, I Mcvey, DS Richt, JA AF Faburay, Bonto Wilson, William C. Gaudreault, Natasha N. Davis, A. Sally Shivanna, Vinay Bawa, Bhupinder Sunwoo, Sun Young Ma, Wenjun Drolet, Barbara S. Morozov, Igor Mcvey, D. Scott Richt, Juergen A. TI A Recombinant Rift Valley Fever Virus Glycoprotein Subunit Vaccine Confers Full Protection against Rift Valley Fever Challenge in Sheep SO SCIENTIFIC REPORTS LA English DT Article ID MONOCLONAL-ANTIBODIES; IMMUNE-RESPONSES; EFFICACY; SAFETY; EXPRESSION; STRAIN; NSS; ATTENUATION; SMITHBURN; PROTEINS AB Rift Valley fever virus (RVFV) is a mosquito-borne zoonotic pathogen causing disease outbreaks in Africa and the Arabian Peninsula. The virus has great potential for transboundary spread due to the presence of competent vectors in non-endemic areas. There is currently no fully licensed vaccine suitable for use in livestock or humans outside endemic areas. Here we report the evaluation of the efficacy of a recombinant subunit vaccine based on the RVFV Gn and Gc glycoproteins. In a previous study, the vaccine elicited strong virus neutralizing antibody responses in sheep and was DIVA (differentiating naturally infected from vaccinated animals) compatible. In the current efficacy study, a group of sheep (n=5) was vaccinated subcutaneously with the glycoprotein-based subunit vaccine candidate and then subjected to heterologous challenge with the virulent Kenya-128B-15 RVFV strain. The vaccine elicited high virus neutralizing antibody titers and conferred complete protection in all vaccinated sheep, as evidenced by prevention of viremia, fever and absence of RVFV-associated histopathological lesions. We conclude that the subunit vaccine platform represents a promising strategy for the prevention and control of RVFV infections in susceptible hosts. C1 [Faburay, Bonto; Gaudreault, Natasha N.; Davis, A. Sally; Shivanna, Vinay; Bawa, Bhupinder; Sunwoo, Sun Young; Ma, Wenjun; Morozov, Igor; Richt, Juergen A.] Kansas State Univ, Coll Vet Med, Dept Diagnost Med & Pathobiol, Manhattan, KS 66506 USA. [Wilson, William C.; Drolet, Barbara S.; Mcvey, D. Scott] ARS, USDA, Arthropod Borne Anim Dis Res Unit, Manhattan, KS USA. RP Faburay, B; Richt, JA (reprint author), Kansas State Univ, Coll Vet Med, Dept Diagnost Med & Pathobiol, Manhattan, KS 66506 USA. EM bfaburay@vet.k-state.edu; jricht@vet.k-state.edu OI Shivanna, Vinay/0000-0002-4625-954X FU Department of Homeland Security Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD) [2010-ST061-AG0001]; Kansas State NBAF Transition Funds FX The authors wish to thank the staff of Kansas State University (KSU) Biosecurity Research Institute, the Comparative Medicine Group, and the staff of the Histopathology Unit at the Veterinary Diagnostic Laboratory. We wish to thank Dane Jasperson, Lindsey Reister (ADABRU-USDA/ARS), Chester McDowell, Abaineh Endalew, Aaron Balogh, Tammy Koopman, Haixia Liu, Maira Cotton-Caballero, Elizabeth Stietzle and Monica Gamez (KSU) for technical assistance. This work was funded by grants of the Department of Homeland Security Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD), Grant No. 2010-ST061-AG0001 and Kansas State NBAF Transition Funds. USDA is an equal opportunity provider and employer. NR 50 TC 2 Z9 2 U1 7 U2 11 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUN 14 PY 2016 VL 6 AR 27719 DI 10.1038/srep27719 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DO3ZT UT WOS:000377722000001 PM 27296136 ER PT J AU Covarrubias-Pazaran, G Diaz-Garcia, L Schlautman, B Deutsch, J Salazar, W Hernandez-Ochoa, M Grygleski, E Steffan, S Iorizzo, M Polashock, J Vorsa, N Zalapa, J AF Covarrubias-Pazaran, Giovanny Diaz-Garcia, Luis Schlautman, Brandon Deutsch, Joseph Salazar, Walter Hernandez-Ochoa, Miguel Grygleski, Edward Steffan, Shawn Iorizzo, Massimo Polashock, James Vorsa, Nicholi Zalapa, Juan TI Exploiting genotyping by sequencing to characterize the genomic structure of the American cranberry through high-density linkage mapping SO BMC GENOMICS LA English DT Article DE Genotyping by sequencing; Imputation; Vaccinium macrocarpon; Pseudo-testcross; Linkage disequilibrium; Segregation distortion; Synteny ID VACCINIUM-MACROCARPON AIT.; PSEUDO-TESTCROSS STRATEGY; QUANTITATIVE TRAIT LOCI; ULTRA-HIGH-DENSITY; EST-SSR MARKERS; GENETIC-MAP; COMPLEX TRAITS; WHOLE-GENOME; RUBUS-IDAEUS; CONSTRUCTION AB Background: The application of genotyping by sequencing (GBS) approaches, combined with data imputation methodologies, is narrowing the genetic knowledge gap between major and understudied, minor crops. GBS is an excellent tool to characterize the genomic structure of recently domesticated (similar to 200 years) and understudied species, such as cranberry (Vaccinium macrocarpon Ait.), by generating large numbers of markers for genomic studies such as genetic mapping. Results: We identified 10842 potentially mappable single nucleotide polymorphisms (SNPs) in a cranberry pseudo-testcross population wherein 5477 SNPs and 211 short sequence repeats (SSRs) were used to construct a high density linkage map in cranberry of which a total of 4849 markers were mapped. Recombination frequency, linkage disequilibrium (LD), and segregation distortion at the genomic level in the parental and integrated linkage maps were characterized for first time in cranberry. SSR markers, used as the backbone in the map, revealed high collinearity with previously published linkage maps. The 4849 point map consisted of twelve linkage groups spanning 1112 cM, which anchored 2381 nuclear scaffolds accounting for similar to 13 Mb of the estimated 470 Mb cranberry genome. Bin mapping identified 592 and 672 unique bins in the parentals and a total of 1676 unique marker positions in the integrated map. Synteny analyses comparing the order of anchored cranberry scaffolds to their homologous positions in kiwifruit, grape, and coffee genomes provided initial evidence of homology between cranberry and closely related species. Conclusions: GBS data was used to rapidly saturate the cranberry genome with markers in a pseudo-testcross population. Collinearity between the present saturated genetic map and previous cranberry SSR maps suggests that the SNP locations represent accurate marker order and chromosome structure of the cranberry genome. SNPs greatly improved current marker genome coverage, which allowed for genome-wide structure investigations such as segregation distortion, recombination, linkage disequilibrium, and synteny analyses. In the future, GBS can be used to accelerate cranberry molecular breeding through QTL mapping and genome-wide association studies (GWAS). C1 [Covarrubias-Pazaran, Giovanny; Diaz-Garcia, Luis; Schlautman, Brandon; Salazar, Walter; Hernandez-Ochoa, Miguel; Zalapa, Juan] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Steffan, Shawn; Zalapa, Juan] Univ Wisconsin, USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. [Diaz-Garcia, Luis] Inst Nacl Invest Agr Forestales & Pecuarias, Aguascalientes, Mexico. [Deutsch, Joseph] Univ Wisconsin, Dept Stat, Madison, WI 53706 USA. [Grygleski, Edward] Valley Corp, Tomah, WI USA. [Iorizzo, Massimo] N Carolina State Univ, Plants Human Hlth Inst, Dept Hort Sci, Kannapolis, NC USA. [Polashock, James] USDA ARS, Genet Improvement Fruits & Vegetables Lab, Chatsworth, NJ USA. [Vorsa, Nicholi] Rutgers State Univ, Blueberry & Cranberry Res & Extens Ctr, Chatsworth, NJ USA. RP Zalapa, J (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA.; Zalapa, J (reprint author), Univ Wisconsin, USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. EM jezalapa@wisc.edu FU USDA-SRCI [2008-51180-04878]; USDA-NIFA-AFRI Competitive Grant [USDA-NIFA-2013-67013-21107]; USDA-ARS [3655-21220-001-00]; WI-DATCP (SCBG Project) [14-002]; National Science Foundation [DBI-1228280]; Ocean Spray Cranberries, Inc.; NJ Cranberry and Blueberry Research Council; Wisconsin Cranberry Growers Association; Cranberry Institute; Frank B. Koller Cranberry Fellowship Fund for Graduate Students; Consejo Nacional de Ciencia y Tecnologia (CONACYT, Mexico); USDA National Institute of Food and Agriculture, Hatch project [1008691] FX This project was supported by USDA-SRCI under Grant 2008-51180-04878; USDA-NIFA-AFRI Competitive Grant USDA-NIFA-2013-67013-21107; USDA-ARS (project no. 3655-21220-001-00 provided to JZ and SS); WI-DATCP (SCBG Project #14-002); National Science Foundation (DBI-1228280); Ocean Spray Cranberries, Inc.; NJ Cranberry and Blueberry Research Council; Wisconsin Cranberry Growers Association; Cranberry Institute. BS was supported by the Frank B. Koller Cranberry Fellowship Fund for Graduate Students; GCP and LDG were supported by the Consejo Nacional de Ciencia y Tecnologia (CONACYT, Mexico); MI was supported by the USDA National Institute of Food and Agriculture, Hatch project 1008691. NR 96 TC 0 Z9 0 U1 14 U2 19 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUN 13 PY 2016 VL 17 AR 451 DI 10.1186/s12864-016-2802-3 PG 16 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DP3FO UT WOS:000378378400001 PM 27295982 ER PT J AU Jones, DC Zheng, WG Huang, S Du, CL Zhao, XF Yennamalli, RM Sen, TZ Nettleton, D Wurtele, ES Li, L AF Jones, Dallas C. Zheng, Wenguang Huang, Sheng Du, Chuanlong Zhao, Xuefeng Yennamalli, Ragothaman M. Sen, Taner Z. Nettleton, Dan Wurtele, Eve S. Li, Ling TI A Clade-Specific Arabidopsis Gene Connects Primary Metabolism and Senescence SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE SAQR; stress; senescence; Arabidopsis; QQS; starch; carbon allocation; AT1G64360 ID LEAF SENESCENCE; PLANT SENESCENCE; ABSCISIC-ACID; NITROGEN REMOBILIZATION; PHOTOOXIDATIVE STRESS; SIGNALING PATHWAYS; DIRIGENT PROTEINS; UNKNOWN FUNCTION; JASMONIC ACID; DAUCUS-CAROTA AB Nearly immobile, plants have evolved new components to be able to respond to changing environments. One example is Qua Quine Starch (QQS, AT3G30720), an Arabidopsis thallana-specific orphan gene that integrates primary metabolism with adaptation to environment changes. SAQR (Senescence Associated and QQS-Related, All G64360), is unique to a Glade within the family Brassicaceae; as such, the gene may have arisen about 20 million years ago. SAQR is up-regulated in QQS RNAi mutant and in the apx1 mutant under light-induced oxidative stress. SAQR plays a role in carbon allocation: overexpression lines of SAQR have significantly decreased starch content; conversely, in a saw 1-DNA knockout (KO) line, starch accumulation is increased. Meta analysis of public microarray data indicates that SAQF? expression is correlated with expression of a subset of genes involved in senescence, defense, and stress responses. SAC:2F? promoter::GUS expression analysis reveals that SAC:2F? expression increases after leaf expansion and photosynthetic capacity have peaked, just prior to visible natural senescence. SAQR is expressed predominantly within leaf and cotyledon vasculature, increasing in intensity as natural senescence continues, and then decreasing prior to death. In contrast, under experimentally induced senescence, SAQR expression increases in vasculature of cotyledons but not in true leaves. In SAQR KO line, the transcript level of the dirigent-like disease resistance gene (AT1G22900) is increased, while that of the Early Light Induced Protein 1 gene (ELIP1, AT3G22840) is decreased. Taken together, these data indicate that SAQR may function in the QQS network, playing a role in integration of primary metabolism with adaptation to internal and environmental changes, specifically those that affect the process of senescence. C1 [Jones, Dallas C.; Zheng, Wenguang; Huang, Sheng; Yennamalli, Ragothaman M.; Sen, Taner Z.; Wurtele, Eve S.; Li, Ling] Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA USA. [Du, Chuanlong; Nettleton, Dan] Iowa State Univ, Dept Stat, Ames, IA USA. [Zhao, Xuefeng] Iowa State Univ, Laurence H Baker Ctr Bioinformat & Biol Stat, Ames, IA USA. [Yennamalli, Ragothaman M.; Sen, Taner Z.] ARS, Corn Insects & Crop Genet Res Unit, USDA, Ames, IA USA. [Wurtele, Eve S.; Li, Ling] Iowa State Univ, Ctr Metabol Biol, Ames, IA USA. [Zhao, Xuefeng] Iowa State Univ, Informat Technol, Coll Liberal Arts & Sci, Ames, IA USA. [Yennamalli, Ragothaman M.] Jaypee Univ Informat Technol, Dept Biotechnol & Bioinformat, Waknaghat, India. [Huang, Sheng] Guangxi Univ, Coll Life Sci & Technol, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning, Peoples R China. RP Li, L (reprint author), Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA USA.; Li, L (reprint author), Iowa State Univ, Ctr Metabol Biol, Ames, IA USA. EM liling@iastate.edu OI Wurtele, Eve/0000-0003-1552-9495 FU National Science Foundation [MCB-0951170]; Center for Metabolic Biology at Iowa State University; Iowa State University Alliance for Graduate Education; National Science Foundation FX LL and LW are grateful for the funding from the National Science Foundation under award number MCB-0951170 and support from the Center for Metabolic Biology at Iowa State University (to :1,1). DJ was supported by the Iowa State University Alliance for Graduate Education and the Professoriate (AGEP) program from the National Science Foundation. NR 101 TC 0 Z9 0 U1 12 U2 13 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JUN 12 PY 2016 VL 7 AR 983 DI 10.3389/fpls.2016.00983 PG 18 WC Plant Sciences SC Plant Sciences GA DQ7RM UT WOS:000379405200001 PM 27462324 ER PT J AU Kim, SG Claassen, R AF Kim, Seung Gyu Claassen, Roger TI Modelling land development processes using a sample selection regression SO APPLIED ECONOMICS LETTERS LA English DT Article DE Land development; sample selection regression; land value tax; parcel fragmentation; R14; R52 AB Land value tax has been thoroughly scrutinized as a potential tool in mitigating urban sprawl. However, most studies that have measured the impact of land value tax on land development have focused mainly either on purely new development on vacant land, or on land development on partially developed land such as subdivision development. This study tries to incorporate these two types of residential development conceptually and empirically to make an accurate assessment of the impact of land value tax on land development. C1 [Kim, Seung Gyu] Kyungpook Natl Univ, Agr Econ, Daegu, South Korea. [Claassen, Roger] USDA, Econ Res Serv, Washington, DC USA. RP Kim, SG (reprint author), Kyungpook Natl Univ, Agr Econ, Daegu, South Korea. EM sgkimwin@knu.ac.kr FU Kyungpook National University FX This research was supported by Kyungpook National University A.S Research Fund, 2013. Views expressed are the authors' alone and do not necessarily correspond to those of Kyungpook National University or U.S. Department of Agriculture. The first author thanks University of Tennessee for the graduate assistantship support in that data used in the analysis were collected during the period. NR 7 TC 0 Z9 0 U1 3 U2 11 PU ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXFORDSHIRE, ENGLAND SN 1350-4851 EI 1466-4291 J9 APPL ECON LETT JI Appl. Econ. Lett. PD JUN 12 PY 2016 VL 23 IS 9 BP 664 EP 668 DI 10.1080/13504851.2015.1095999 PG 5 WC Economics SC Business & Economics GA DG4ZF UT WOS:000372081000012 ER PT J AU Carvalho, LJCB Agustini, MAV Anderson, JV Vieira, EA de Souza, CRB Chen, SB Schaal, BA Silva, JP AF Carvalho, Luiz J. C. B. Agustini, Marco A. V. Anderson, James V. Vieira, Eduardo A. de Souza, Claudia R. B. Chen, Songbi Schaal, Barbara A. Silva, Joseane P. TI Natural variation in expression of genes associated with carotenoid biosynthesis and accumulation in cassava (Manihot esculenta Crantz) storage root SO BMC PLANT BIOLOGY LA English DT Article DE Cassava storage root; Carotenoid; beta-carotene; HPLC-DAD; Microarray; qRT_PCR ID CHLOROPLAST DIVISION; SINGLET OXYGEN; ABSCISIC-ACID; PLANTS; STRESS; RESPONSES; POTATO; CULTIVARS; PRODUCTS; RESOURCE AB Background: Cassava (Manihot esculenta Crantz) storage root provides a staple food source for millions of people worldwide. Increasing the carotenoid content in storage root of cassava could provide improved nutritional and health benefits. Because carotenoid accumulation has been associated with storage root color, this study characterized carotenoid profiles, and abundance of key transcripts associated with carotenoid biosynthesis, from 23 landraces of cassava storage root ranging in color from white-to-yellow-to-pink. This study provides important information to plant breeding programs aimed at improving cassava storage root nutritional quality. Results: Among the 23 landraces, five carotenoid types were detected in storage root with white color, while carotenoid types ranged from 1 to 21 in storage root with pink and yellow color. The majority of storage root in these landraces ranged in color from pale-to-intense yellow. In this color group, total beta-carotene, containing all-E-, 9-Z-, and 13-Z-beta-carotene isomers, was the major carotenoid type detected, varying from 26.13 to 76.72 %. Although no a-carotene was observed, variable amounts of a alpha-ring derived xanthophyll, lutein, was detected; with greater accumulation of a-ring xanthophylls than of beta-ring xanthophyll. Lycopene was detected in a landrace (Cas51) with pink color storage root, but it was not detected in storage root with yellow color. Based on microarray and qRT-PCR analyses, abundance of transcripts coding for enzymes involved in carotenoid biosynthesis were consistent with carotenoid composition determined by contrasting HPLC-Diode Array profiles from storage root of landraces IAC12, Cas64, and Cas51. Abundance of transcripts encoding for proteins regulating plastid division were also consistent with the observed differences in total beta-carotene accumulation. Conclusions: Among the 23 cassava landraces with varying storage root color and diverse carotenoid types and profiles, landrace Cas51 (pink color storage root) had low LYCb transcript abundance, whereas landrace Cas64 (intense yellow storage root) had decreased HYb transcript abundance. These results may explain the increased amounts of lycopene and total beta-carotene observed in landraces Cas51 and Cas64, respectively. Overall, total carotenoid content in cassava storage root of color class representatives were associated with spatial patterns of secondary growth, color, and abundance of transcripts linked to plastid division. Finally, a partial carotenoid biosynthesis pathway is proposed. C1 [Carvalho, Luiz J. C. B.; Agustini, Marco A. V.; Silva, Joseane P.] EMBRAPA Genet Resources & Biotechnol, Brasilia, DF, Brazil. [Anderson, James V.] USDA ARS, Sunflower & Plant Biol Res Unit, Fargo, ND USA. [Vieira, Eduardo A.] EMBRAPA Cerrados, Brasilia, DF, Brazil. [de Souza, Claudia R. B.] Fed Univ Para, Belem, Para, Brazil. [Chen, Songbi] Chinese Acad Trop Agr, Trop Crops Genet Resources Inst, Haikou, Hainan, Peoples R China. [Schaal, Barbara A.] Washington Univ, Dept Biol, Campus Box 1137, St Louis, MO 63130 USA. RP Carvalho, LJCB (reprint author), EMBRAPA Genet Resources & Biotechnol, Brasilia, DF, Brazil.; Chen, SB (reprint author), Chinese Acad Trop Agr, Trop Crops Genet Resources Inst, Haikou, Hainan, Peoples R China. EM luiz.castelo@embrapa.br; songbichen@yahoo.com FU Rockefeller Foundation [RF96010 25, RF9707 26]; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, CNPq [480410.2001-1]; Programa Nacional de Pesquisa em Biotecnologia - CENARGEN [060302058]; USDA_ARS; IAEA [13188]; National Nature Science Foundation of China (NSFC) [31271776]; NSFC-CGIAR International (Regional) Cooperation and Exchange Programs [31361140366] FX Special acknowledgements are extended for financial and technical support provided by The Rockefeller Foundation (RF96010#25 and RF9707#26 for the search for diversity in landraces); Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, CNPq, (Grant # 480410.2001-1 for the functional genomics work); Programa Nacional de Pesquisa em Biotecnologia - CENARGEN (Project No 060302058 for carotenoid analysis and EMBRAPA_LABEX_USA collaborative program); USDA_ARS for providing lab facilities in Fargo, ND (USA) and providing access to Euphorbiaceae microarray cDNA chip technology; IAEA (contract # 13188 for funds supplied for gene expression analysis); National Nature Science Foundation of China (NSFC grant number 31271776); and NSFC-CGIAR International (Regional) Cooperation and Exchange Programs (grant number 31361140366). The germplasm derived from this study was deposited in CIAT facilities and donated to the Harvest-Plus Initiative for dissemination worldwide, which is also acknowledged. NR 55 TC 0 Z9 0 U1 7 U2 7 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD JUN 10 PY 2016 VL 16 AR 133 DI 10.1186/s12870-016-0826-0 PG 23 WC Plant Sciences SC Plant Sciences GA DQ0FB UT WOS:000378872800003 PM 27286876 ER PT J AU Eves-van den Akker, S Laetsch, DR Thorpe, P Lilley, CJ Danchin, EGJ Da Rocha, M Rancurel, C Holroyd, NE Cotton, JA Szitenberg, A Grenier, E Montarry, J Mimee, B Duceppe, MO Boyes, I Marvin, JMC Jones, LM Yusup, HB Lafond-Lapalme, J Esquibet, M Sabeh, M Rott, M Overmars, H Finkers-Tomczak, A Smant, G Koutsovoulos, G Blok, V Mantelin, S Cock, PJA Phillips, W Henrissat, B Urwin, PE Blaxter, M Jones, JT AF Eves-van den Akker, Sebastian Laetsch, Dominik R. Thorpe, Peter Lilley, Catherine J. Danchin, Etienne G. J. Da Rocha, Martine Rancurel, Corinne Holroyd, Nancy E. Cotton, James A. Szitenberg, Amir Grenier, Eric Montarry, Josselin Mimee, Benjamin Duceppe, Marc-Olivier Boyes, Ian Marvin, Jessica M. C. Jones, Laura M. Yusup, Hazijah B. Lafond-Lapalme, Joel Esquibet, Magali Sabeh, Michael Rott, Michael Overmars, Hein Finkers-Tomczak, Anna Smant, Geert Koutsovoulos, Georgios Blok, Vivian Mantelin, Sophie Cock, Peter J. A. Phillips, Wendy Henrissat, Bernard Urwin, Peter E. Blaxter, Mark Jones, John T. TI The genome of the yellow potato cyst nematode, Globodera rostochiensis, reveals insights into the basis of parasitism and virulence SO GENOME BIOLOGY LA English DT Article DE Plant-parasitic nematode; Genome sequence; Virulence; Effectors; Horizontal gene transfer ID HORIZONTAL GENE-TRANSFER; PHYTONEMATODE HETERODERA-GLYCINES; GLAND SECRETORY GRANULES; PLANT PARASITISM; FUNCTIONAL-CHARACTERIZATION; DIFFERENTIAL EXPRESSION; PHYTOPHTHORA-INFESTANS; MELOIDOGYNE-INCOGNITA; EUROPEAN POPULATIONS; ESOPHAGEAL GLANDS AB Background: The yellow potato cyst nematode, Globodera rostochiensis, is a devastating plant pathogen of global economic importance. This biotrophic parasite secretes effectors from pharyngeal glands, some of which were acquired by horizontal gene transfer, to manipulate host processes and promote parasitism. G. rostochiensis is classified into pathotypes with different plant resistance-breaking phenotypes. Results: We generate a high quality genome assembly for G. rostochiensis pathotype Ro1, identify putative effectors and horizontal gene transfer events, map gene expression through the life cycle focusing on key parasitic transitions and sequence the genomes of eight populations including four additional pathotypes to identify variation. Horizontal gene transfer contributes 3.5 % of the predicted genes, of which approximately 8.5 % are deployed as effectors. Over one-third of all effector genes are clustered in 21 putative 'effector islands' in the genome. We identify a dorsal gland promoter element motif (termed DOG Box) present upstream in representatives from 26 out of 28 dorsal gland effector families, and predict a putative effector superset associated with this motif. We validate gland cell expression in two novel genes by in situ hybridisation and catalogue dorsal gland promoter element-containing effectors from available cyst nematode genomes. Comparison of effector diversity between pathotypes highlights correlation with plant resistance-breaking. Conclusions: These G. rostochiensis genome resources will facilitate major advances in understanding nematode plant-parasitism. Dorsal gland promoter element-containing effectors are at the front line of the evolutionary arms race between plant and parasite and the ability to predict gland cell expression a priori promises rapid advances in understanding their roles and mechanisms of action. C1 [Eves-van den Akker, Sebastian] Univ Dundee, Coll Life Sci, Div Plant Sci, Dundee DD1 5EH, Scotland. [Laetsch, Dominik R.; Koutsovoulos, Georgios; Blaxter, Mark] Univ Edinburgh, Inst Evolutionary Biol, Edinburgh EH9 3FL, Midlothian, Scotland. [Thorpe, Peter; Blok, Vivian; Mantelin, Sophie; Jones, John T.] James Hutton Inst, Dundee Effector Consortium, Cell & Mol Sci Grp, Dundee DD2 5DA, Scotland. [Lilley, Catherine J.; Marvin, Jessica M. C.; Jones, Laura M.; Yusup, Hazijah B.; Urwin, Peter E.] Univ Leeds, Ctr Plant Sci, Leeds LS2 9JT, W Yorkshire, England. [Danchin, Etienne G. J.; Da Rocha, Martine; Rancurel, Corinne] Univ Nice Sophia Antipolis, CNRS, INRA, Inst Sophia Agrobiotech,UMR 1355 7254, F-06900 Sophia Antipolis, France. [Holroyd, Nancy E.; Cotton, James A.] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England. [Szitenberg, Amir] Univ Hull, Sch Biol Biomed & Environm Sci, Kingston Upon Hull HU6 7RX, N Humberside, England. [Grenier, Eric; Montarry, Josselin; Esquibet, Magali] INRA, IGEPP, UMR1349, F-35653 Le Rheu, France. [Mimee, Benjamin; Duceppe, Marc-Olivier; Lafond-Lapalme, Joel; Sabeh, Michael] Agr & Agri Food Canada, Hort Res & Dev Ctr, St Jean, PQ J3B 3E6, Canada. [Boyes, Ian] Canadian Food Inspect Agcy, Sidney Lab, Sidney, BC V8L 1H3, Canada. [Overmars, Hein; Finkers-Tomczak, Anna; Smant, Geert] Wageningen Univ, Dept Plant Sci, Nematol Lab, NL-6708 PB Wageningen, Netherlands. [Cock, Peter J. A.] James Hutton Inst, Informat & Computat Sci Grp, Dundee, Scotland. [Phillips, Wendy] USDA ARS, Hort Crops Res Lab, Corvallis, OR USA. [Henrissat, Bernard] Aix Marseille Univ, AFMB, INRA, USC 1408,CNRS UMR 7257, F-13288 Marseille, France. [Henrissat, Bernard] King Abdulaziz Univ, Dept Biol Sci, Jeddah 21413, Saudi Arabia. [Jones, John T.] Univ St Andrews, Sch Biol, St Andrews KY16 9TZ, Fife, Scotland. RP Eves-van den Akker, S (reprint author), Univ Dundee, Coll Life Sci, Div Plant Sci, Dundee DD1 5EH, Scotland. EM s.evesvandenakker@dundee.ac.uk RI Grenier, Eric/Q-7349-2016; Cotton, James/B-8806-2008; Danchin, Etienne/A-6648-2008; Blaxter, Mark/B-4113-2010; Fac Sci, KAU, Biol Sci Dept/L-4228-2013; OI Grenier, Eric/0000-0002-2260-1101; Cotton, James/0000-0001-5475-3583; Danchin, Etienne/0000-0003-4146-5608; Blaxter, Mark/0000-0003-2861-949X; Cock, Peter/0000-0001-9513-9993 FU BBSRC [BB/M014207/1, BB/F000642/1, BB/F00334X/1]; James Hutton Institute; School of Biological Sciences, University of Edinburgh; BBSRC PhD studentship; Scottish Government; Wellcome Trust through its core funding of the Wellcome Trust Sanger Institute [098051]; Canadian Safety and Security Program [CRTI09_462RD] FX SE-vdA is supported by BBSRC grant BB/M014207/1. Sequencing was funded by BBSRC grant BB/F000642/1 to the University of Leeds and grant BB/F00334X/1 to the Wellcome Trust Sanger Institute). DRL was supported by a fellowship from The James Hutton Institute and the School of Biological Sciences, University of Edinburgh. GK was supported by a BBSRC PhD studentship. The James Hutton Institute receives funding from the Scottish Government. JAC and NEH are supported by the Wellcome Trust through its core funding of the Wellcome Trust Sanger Institute (grant 098051). This work was also supported by funding from the Canadian Safety and Security Program, project number CRTI09_462RD. NR 102 TC 2 Z9 2 U1 10 U2 27 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1474-760X J9 GENOME BIOL JI Genome Biol. PD JUN 10 PY 2016 VL 17 AR 124 DI 10.1186/s13059-016-0985-1 PG 23 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DQ0OQ UT WOS:000378899700001 PM 27286965 ER PT J AU Nemchinov, LG Boutanaev, AM Postnikova, OA AF Nemchinov, Lev G. Boutanaev, Alexander M. Postnikova, Olga A. TI Virus-induced gene silencing of the RPC5-like subunit of RNA polymerase III caused pleiotropic effects in Nicotiana benthamiana SO SCIENTIFIC REPORTS LA English DT Article ID TOBACCO-MOSAIC-VIRUS; ARABIDOPSIS-THALIANA; PHOTOSYSTEM-II; PROTEIN; PLANTS; TRANSCRIPTION; BIOSYNTHESIS; EXPRESSION; REGULATORS; RESPONSES AB In eukaryotic cells, RNA polymerase III is highly conserved and transcribes housekeeping genes such as ribosomal 5S rRNA, tRNA and other small RNAs. The RPC5-like subunit is one of the 17 subunits forming RNAPIII and its exact functional roles in the transcription are poorly understood. In this work, we report that virus-induced gene silencing of transcripts encoding a putative RPC5-like subunit of the RNA Polymerase III in a model species Nicotiana benthamiana had pleiotropic effects, including but not limited to severe dwarfing appearance, chlorosis, nearly complete reduction of internodes and abnormal leaf shape. Using transcriptomic analysis, we identified genes and pathways affected by RPC5 silencing and thus presumably related to the cellular roles of the subunit as well as to the downstream cascade of reactions in response to partial loss of RNA Polymerase III function. Our results suggest that silencing of the RPC5L in N. benthamiana disrupted not only functions commonly associated with the core RNA Polymerase III transcripts, but also more diverse cellular processes, including responses to stress. We believe this is the first demonstration that activity of the RPC5 subunit is critical for proper functionality of RNA Polymerase III and normal plant development. C1 [Nemchinov, Lev G.; Postnikova, Olga A.] ARS, USDA, Beltsville Agr Res Ctr, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. [Boutanaev, Alexander M.] Russian Acad Sci, Inst Basic Biol Problems, 2 Inst St, Pushchino 142292, Moscow Region, Russia. RP Nemchinov, LG (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. EM Lev.Nemchinov@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service FX We thank Jonathan Shao of the Molecular Plant Pathology Laboratory, USDA-ARS for his assistance with handling high throughput data, David Fleisher of Crop Systems & Global Change Laboratory, USDA-ARS, for his help with measurements of photosynthetic rates and John Hartung of the Molecular Plant Pathology Laboratory, USDA-ARS for editing the manuscript and insightful comments. This work was supported by the United States Department of Agriculture, Agricultural Research Service. NR 63 TC 0 Z9 0 U1 3 U2 3 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUN 10 PY 2016 VL 6 AR 27785 DI 10.1038/srep27785 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DO4AF UT WOS:000377723200001 PM 27282827 ER PT J AU Schutta, C Barrera, J Pisano, M Zsak, L Grubman, MJ Mayr, GA Moraes, MP Kamicker, BJ Brake, DA Ettyreddy, D Brough, DE Butman, BT Neilan, JG AF Schutta, Christopher Barrera, Jose Pisano, Melia Zsak, Laszlo Grubman, Marvin J. Mayr, Gregory A. Moraes, Mauro P. Kamicker, Barbara J. Brake, David A. Ettyreddy, Damodar Brough, Douglas E. Butman, Bryan T. Neilan, John G. TI Multiple efficacy studies of an adenovirus-vectored foot-and-mouth disease virus serotype A24 subunit vaccine in cattle using homologous challenge SO VACCINE LA English DT Article DE Foot-and-mouth disease virus; FMDV serotype A24 Cruzeiro; Replication-deficient human adenovirus vectored vaccine; DIVA; Vaccine efficacy ID KIDNEY-CELL LINE; PROTECTION; FMDV; ANTIBODIES; REGIONS AB The safety and efficacy of an experimental, replication-deficient, human adenovirus-vectored foot-and-mouth disease virus (FMDV) serotype A24 Cruzeiro capsid-based subunit vaccine (AdtA24) was examined in eight independent cattle studies. AdtA24 non-adjuvanted vaccine was administered intramuscularly to a total of 150 steers in doses ranging from approximately 1.0 x 10(8) to 2.1 x 10(11) particle units per animal. No detectable local or systemic reactions were observed after vaccination. At 7 days post-vaccination (dpv), vaccinated and control animals were challenged with FMDV serotype A24 Cruzeiro via the intradermal lingual route. Vaccine efficacy was measured by FMDV A24 serum neutralizing titers and by protection from clinical disease and viremia after challenge. The results of eight studies demonstrated a strong correlation between AdtA24 vaccine dose and protection from clinical disease (R-2 = 0.97) and viremia (R-2 = 0.98). There was also a strong correlation between FMDV A24 neutralization titers on day of challenge and protection from clinical disease (R-2= 0.99). Vaccination with AdtA24 enabled differentiation of infected from vaccinated animals (DIVA) as demonstrated by the absence of antibodies to the FMDV nonstructural proteins in vaccinates prior to challenge. Lack of AdtA24 vaccine shedding after vaccination was indicated by the absence of neutralizing antibody titers to both the adenovector and FMDV A24 Cruzeiro in control animals after co-mingling with vaccinated cattle for three to four weeks. In summary, a non-adjuvanted AdtA24 experimental vaccine was shown to be safe, immunogenic, consistently protected cattle at 7 dpv against direct, homologous FMDV challenge, and enabled differentiation of infected from vaccinated cattle prior to challenge. Published by Elsevier Ltd. C1 [Schutta, Christopher; Zsak, Laszlo; Neilan, John G.] US Dept Homeland Secur, Sci & Technol Directorate, Plum Isl Anim Dis Ctr, POB 848, Greenport, NY 11944 USA. [Barrera, Jose; Pisano, Melia] McConnell Grp Inc, Plum Isl Anim Dis Ctr, POB 848, Greenport, NY 11944 USA. [Pisano, Melia] Oak Ridge Inst Sci & Educ, PIADC Res Participat Program, Oak Ridge, TN USA. [Grubman, Marvin J.; Mayr, Gregory A.; Moraes, Mauro P.] ARS, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, USDA, POB 848, Greenport, NY 11944 USA. [Barrera, Jose; Pisano, Melia; Kamicker, Barbara J.; Brake, David A.] Leidos Inc, Plum Isl Anim Dis Ctr, POB 848, Greenport, NY 11944 USA. [Ettyreddy, Damodar; Brough, Douglas E.; Butman, Bryan T.] GenVec Inc, 910 Clopper Rd,Suite 220N, Gaithersburg, MD 20878 USA. [Zsak, Laszlo] ARS, Southeast Poultry Res Lab, Southeast Area, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. [Mayr, Gregory A.] USDA, Plum Isl Anim Dis Ctr, Anim & Plant Hlth Inspect Serv, POB 848, Greenport, NY 11944 USA. [Moraes, Mauro P.] Ceva Biomune, 8906 Rosehill Rd, Lenexa, KS 66215 USA. RP Neilan, JG (reprint author), US Dept Homeland Secur, Sci & Technol Directorate, Plum Isl Anim Dis Ctr, POB 848, Greenport, NY 11944 USA. EM john.neilan@st.dhs.gov FU Science and Technology Directorate of the U.S. Department of Homeland Security (DHS ST) [HSHQDC-07-9-00004, GS-23F-8006H, HSHQDC-8-C-00011, HSHQPD-07-X-00003]; U.S. Department of Energy (DOE); U.S. DHS; DOE [DE-AC05-06OR23100]; DHS S&T Agriculture Defense Branch FX We thank K. Addorisio, A. Zsak, W. Hurtle, T. Hester, and K. Floyd for their technical assistance. We thank the PIADC Animal Research Branch for their professional support and assistance and M. Rasmussen for critical review of this manuscript. GenVec, Inc., Leidos, Inc., The McConnell Group, Inc., and M. Moraes were funded through separate agreements with the Science and Technology Directorate of the U.S. Department of Homeland Security (DHS S&T) under Award Numbers HSHQDC-07-9-00004, GS-23F-8006H, HSHQDC-8-C-00011, and HSHQPD-07-X-00003. This research was supported in part by an appointment of M. Pisano to the PIADC Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and the U.S. DHS. ORISE is managed by ORAU under DOE contract number DE-AC05-06OR23100. DHS S&T Agriculture Defense Branch, as the funding source, had no involvement in the study design; collection, analysis and interpretation of data; writing of the report; and in the decision to submit the article for publication. All opinions expressed in this paper are the authors' and do not necessarily reflect the policies and views of DHS, ARS, DOE, or ORAU/ORISE. NR 19 TC 2 Z9 2 U1 1 U2 1 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X EI 1873-2518 J9 VACCINE JI Vaccine PD JUN 8 PY 2016 VL 34 IS 27 BP 3214 EP 3220 DI 10.1016/j.vaccine.2015.12.018 PG 7 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA DP7GT UT WOS:000378667900030 PM 26707216 ER PT J AU Shvedova, AA Kisin, ER Yanamala, N Farcas, MT Menas, AL Williams, A Fournier, PM Reynolds, JS Gutkin, DW Star, A Reiner, RS Halappanavar, S Kagan, VE AF Shvedova, Anna A. Kisin, Elena R. Yanamala, Naveena Farcas, Mariana T. Menas, Autumn L. Williams, Andrew Fournier, Philip M. Reynolds, Jeffrey S. Gutkin, Dmitriy W. Star, Alexander Reiner, Richard S. Halappanavar, Sabina Kagan, Valerian E. TI Gender differences in murine pulmonary responses elicited by cellulose nanocrystals SO PARTICLE AND FIBRE TOXICOLOGY LA English DT Article DE Cellulose; Gender differences; Pulmonary toxicity; Inflammation; Oxidative stress; mRNA expression ID NECROSIS-FACTOR-ALPHA; LUNG-FUNCTION; MANNOSE RECEPTOR; RESPIRATORY SYMPTOMS; ASEPTIC INFLAMMATION; MACROPHAGE FUSION; ESTROGEN-RECEPTOR; GENE-EXPRESSION; MICROARRAY DATA; SEX-DIFFERENCES AB Background: Cellulose-based materials have been used for centuries to manufacture different goods derived from forestry and agricultural sources. In the growing field of nanocellulose applications, its uniquely engineered properties are instrumental for inventive products coming to competitive markets. Due to their high aspect ratio and stiffness, it is speculated that cellulose nanocrystals (CNC) may cause similar pulmonary toxicity as carbon nanotubes and asbestos, thus posing a potential negative impact on public health and the environment. Methods: The present study was undertaken to investigate the pulmonary outcomes induced by repeated exposure to respirable CNC. C57BL/6 female and male mice were exposed by pharyngeal aspiration to CNC (40 mu g/mouse) 2 times a week for 3 weeks. Several biochemical endpoints and pathophysiological outcomes along with gene expression changes were evaluated and compared in the lungs of male and female mice. Results: Exposure to respirable CNC caused pulmonary inflammation and damage, induced oxidative stress, elevated TGF-beta and collagen levels in lung, and impaired pulmonary functions. Notably, these effects were markedly more pronounced in females compared to male mice. Moreover, sex differences in responses to pulmonary exposure to CNC were also detected at the level of global mRNA expression as well as in inflammatory cytokine/chemokine activity. Conclusions: Overall, our results indicate that there are considerable differences in responses to respirable CNC based on gender with a higher pulmonary toxicity observed in female mice. C1 [Shvedova, Anna A.; Kisin, Elena R.; Yanamala, Naveena; Farcas, Mariana T.; Menas, Autumn L.] NIOSH, Hlth Effects Lab Div, Exposure Assessment Branch, 1095 Willowdale Rd, Morgantown, WV 26505 USA. [Shvedova, Anna A.] WVU, Dept Physiol & Pharmacol, Sch Med, Morgantown, WV USA. [Reynolds, Jeffrey S.] NIOSH CDC, Engn & Controls Technol Branch, Morgantown, WV USA. [Williams, Andrew; Halappanavar, Sabina] Hlth Canada, Environm & Radiat Hlth Sci Directorate, Ottawa, ON K1A0 K9, Canada. [Fournier, Philip M.; Star, Alexander] Univ Pittsburgh, Dept Chem, Pittsburgh, PA USA. [Gutkin, Dmitriy W.] Univ Pittsburgh, Dept Pathol, Pittsburgh, PA USA. [Reiner, Richard S.] USDA, Forest Prod Lab, Forest Serv, Madison, WI 53705 USA. [Kagan, Valerian E.] Univ Pittsburgh, Free Radical Ctr, Pittsburgh, PA USA. [Kagan, Valerian E.] Univ Pittsburgh, Dept Environm & Occupat Hlth, Pittsburgh, PA USA. RP Shvedova, AA (reprint author), NIOSH, Hlth Effects Lab Div, Exposure Assessment Branch, 1095 Willowdale Rd, Morgantown, WV 26505 USA.; Shvedova, AA (reprint author), WVU, Dept Physiol & Pharmacol, Sch Med, Morgantown, WV USA. EM ats1@cdc.gov FU NTRC [939011K]; NIOSH [OH008282]; NIH [R01ES019304] FX This research was supported by NTRC 939011K, NIOSH OH008282 and NIH R01ES019304. NR 110 TC 5 Z9 5 U1 5 U2 9 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1743-8977 J9 PART FIBRE TOXICOL JI Part. Fibre Toxicol. PD JUN 8 PY 2016 VL 13 AR 28 DI 10.1186/s12989-016-0140-x PG 20 WC Toxicology SC Toxicology GA DP3XZ UT WOS:000378430800001 PM 27278671 ER PT J AU Fu, XY Shen, XH Finnan, EG Haytowitz, DB Booth, SL AF Fu, Xueyan Shen, Xiaohua Finnan, Emily G. Haytowitz, David B. Booth, Sarah L. TI Measurement of Multiple Vitamin K Forms in Processed and Fresh-Cut Pork Products in the US Food Supply SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE vitamin K; phylloquinone; menaquinones; pork; sausage; food composition ID PERFORMANCE LIQUID-CHROMATOGRAPHY; CORONARY-HEART-DISEASE; PHYLLOQUINONE VITAMIN-K-1; MENAQUINONE-4 VITAMIN-K-2; TISSUE MENAQUINONE-4; RATS; DAIRY; COAGULATION; CONVERSION; BACTERIA AB Vitamin K food composition data have historically been limited to plant-based phylloquinone (vitamin K-1). The purpose of this study was to expand analysis of vitamin K to animal products and to measure phylloquinone and 10 forms of menaquinones (vitamin K-2) in processed and fresh-cut pork products. Nationally representative samples of processed pork products (n = 28) were obtained through USDAs National Food and Nutrition Analysis Program, and fresh pork (six cuts; n = 5 per cut) and bacon (n = 4) were purchased from local retail outlets. All samples were analyzed by high-performance liquid chromatography (phylloquinone and menaquinone-4) and atmospheric-pressure chemical ionizationliquid chromatographymass spectrometry (menaquinone-5 to menaquinone-13). Although low in phylloquinone (<2.1 +/- 0.5 mu g of phylloquinone per 100 g), all processed pork products and fresh pork cuts contained menaquinone-4, menaquinone-10, and menaquinone-11 (range: [35.1 +/- 11.0][534 +/- 89.0] mu g of menaquinones per 100 g). The total menaquinone contents of processed pork products were correlated with fat contents (r = 0.935). In summary, processed and fresh-cut pork products are a rich dietary source of menaquinones that are currently unaccounted for in assessment of vitamin K in the food supply. C1 [Fu, Xueyan; Shen, Xiaohua; Finnan, Emily G.; Booth, Sarah L.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. [Haytowitz, David B.] USDA ARS, Beltsville Human Nutr Res Ctr, 10300 Baltimore Ave,Bldg 005 BARC West, Beltsville, MD 20705 USA. RP Fu, XY (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. FU USDA, Agricultural Research Service [58-1950-0-014, 58-1235-1-193]; National Pork Board (NPB) [14-100] FX This work was supported by the USDA, Agricultural Research Service under Cooperative Agreement nos. 58-1950-0-014 and 58-1235-1-193 and the National Pork Board (NPB no. 14-100). NR 27 TC 0 Z9 0 U1 6 U2 8 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 8 PY 2016 VL 64 IS 22 BP 4531 EP 4535 DI 10.1021/acs.jafc.6b00938 PG 5 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DO2XB UT WOS:000377643200010 PM 27191033 ER PT J AU Liu, QM Yang, Y Maleki, SJ Alcocer, M Xu, SS Shi, CL Cao, MJ Liu, GM AF Liu, Qing-Mei Yang, Yang Maleki, Soheila J. Alcocer, Marcos Xu, Sha-Sha Shi, Chao-Lan Cao, Min-Jie Liu, Guang-Ming TI Anti-Food Allergic Activity of Sulfated Polysaccharide from Gracilaria lemaneiformis is Dependent on Immunosuppression and Inhibition of p38 MAPK SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE sulfated polysaccharide from Gracilaria lemaneiformis; food allergy; immunosuppression; p38 MAPK ID MAST-CELL ACTIVATION; T-CELLS; PORPHYRA-HAITANENSIS; SIGNALING PATHWAYS; SENSITIZED MICE; MARINE-ALGAE; BALB/C MICE; TROPOMYOSIN; HYPERSENSITIVITY; PATHOGENESIS AB Polysaccharides from Gracilaria lemaneiformis in particular possess various bioactive functions, but their antiallergic activity remains incompletely defined. Sulfated polysaccharide from Gracilaria lemaneiformis (GLSP) was obtained by water extraction and ethanol precipitation followed by column chromatography. BALB/c mice, RBL-2H3, and KU812 cells were used for verifying the anti food allergic activity of GLSP. According to the results of mice experiment, GLSP was able to alleviate allergy symptoms, to reduce TM-specific IgE and IgG1, to suppress Th2 cell polarization, and to promote the function of regulatory T (Treg) cells. In addition, GLSP had the ability to inhibit the function of RBL-2H3 cells. Furthermore, GLSP inhibited the activation of KU812 via suppression of p38 mitogen-activated protein kinase (MAPK). In conclusion, immunosuppression as well as the reduction in the level of p38 MAPK may contribute to GLSP's putative activity against food allergy. GLSP may be used as a functional food component for allergic patients. C1 [Liu, Qing-Mei; Yang, Yang; Xu, Sha-Sha; Shi, Chao-Lan; Cao, Min-Jie; Liu, Guang-Ming] Jimei Univ, Fujian Collaborat Innovat Ctr Exploitat & Utiliza, Fujian Prov Engn Technol Res Ctr Marine Funct Foo, Coll Food & Biol Engn,Xiamen Key Lab Marine Funct, 43 Yindou Rd, Xiamen 361021, Fujian, Peoples R China. [Maleki, Soheila J.] USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [Alcocer, Marcos] Univ Nottingham, Sch Biosci, Sutton Bonington Campus, Loughborough LE12 5RD, Leics, England. RP Liu, GM (reprint author), Jimei Univ, Fujian Collaborat Innovat Ctr Exploitat & Utiliza, Fujian Prov Engn Technol Res Ctr Marine Funct Foo, Coll Food & Biol Engn,Xiamen Key Lab Marine Funct, 43 Yindou Rd, Xiamen 361021, Fujian, Peoples R China. EM gmliu@jmu.edu.cn FU National Natural Scientific Foundation of China [31171660, U1405214]; Scientific Foundation of Fujian Province [2014N0014]; Marine Scientific Research Special Foundation for Public Sector Program [201505026-03]; Xiamen South Ocean Research Center Project [201405220001] FX This work was supported by grants from the National Natural Scientific Foundation of China (31171660 and U1405214), the Scientific Foundation of Fujian Province (2014N0014), the Marine Scientific Research Special Foundation for Public Sector Program (201505026-03), the Xiamen South Ocean Research Center Project (201405220001). NR 43 TC 3 Z9 3 U1 19 U2 32 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 8 PY 2016 VL 64 IS 22 BP 4536 EP 4544 DI 10.1021/acs.jafc.6b01086 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DO2XB UT WOS:000377643200011 PM 27186807 ER PT J AU Song, J Yang, XP Resende, MFR Neves, LG Todd, J Zhang, JS Comstock, JC Wang, JP AF Song, Jian Yang, Xiping Resende, Marcio F. R., Jr. Neves, Leandro G. Todd, James Zhang, Jisen Comstock, Jack C. Wang, Jianping TI Natural Allelic Variations in Highly Polyploidy Saccharum Complex SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE Saccharum complex; sugarcane; target enrichment sequencing; polyploid; allelic variations; SNP(s) ID MODERN SUGARCANE CULTIVARS; PHYLOGENETIC ANALYSIS; MAXIMUM-LIKELIHOOD; ANALYSIS TOOLKIT; READ ALIGNMENT; SEQUENCE DATA; AFLP MARKERS; GENETIC-MAP; LINKAGE MAP; GENOME AB Sugarcane (Saccharum spp.) is an important sugar and biofuel crop with high polyploid and complex genomes. The Saccharum complex, comprised of Saccharum genus and a few related genera, are important genetic resources for sugarcane breeding. A large amount of natural variation exists within the Saccharum complex. Though understanding their allelic variation has been challenging, it is critical to dissect allelic structure and to identify the alleles controlling important traits in sugarcane. To characterize natural variations in Saccharum complex, a target enrichment sequencing approach was used to assay 12 representative germplasm accessions. In total, 55,946 highly efficient probes were designed based on the sorghum genome and sugarcane unigene set targeting a total of 6 Mb of the sugarcane genome. A pipeline specifically tailored for polyploid sequence variants and genotype calling was established. BWAmem and sorghum genome approved to be an acceptable aligner and reference for sugarcane target enrichment sequence analysis, respectively. Genetic variations including 1,166,066 non -redundant SNPs, 150,421 InDels, 919 gene copy number variations, and 1,257 gene presence/absence variations were detected. SNPs from three different callers (Samtools, Freebayes, and GATK) were compared and the validation rates were nearly 90%. Based on the SNP loci of each accession and their ploidy levels, 999,258 single dosage SNPs were identified and most loci were estimated as largely homozygotes. An average of 34,397 haplotype blocks for each accession was inferred. The highest divergence time among the Saccharum spp. was estimated as 1.2 million years ago (MYA). Saccharum spp, diverged from Erianthus and Sorghum approximately 5 and 6 MYA, respectively. The target enrichment sequencing approach provided an effective way to discover and catalog natural allelic variation in highly polyploid or heterozygous genomes. C1 [Song, Jian; Yang, Xiping; Wang, Jianping] Univ Florida, Dept Agron, Gainesville, FL 32611 USA. [Song, Jian] Dezhou Univ, Coll Life Sci, Dezhou, Peoples R China. [Resende, Marcio F. R., Jr.; Neves, Leandro G.] RAPiD Genom LLC, Gainesville, FL USA. [Todd, James] USDA ARS, Sugarcane Res Unit, Houma, LA 70361 USA. [Todd, James; Comstock, Jack C.] USDA ARS, Sugarcane Field Stn, Canal Point, FL USA. [Zhang, Jisen; Wang, Jianping] Fujian Agr & Forestry Univ, Haixia Inst Sci & Technol, Ctr Genom & Biotechnol, Fuzhou, Peoples R China. [Wang, Jianping] Univ Florida, Genet Inst, Plant Mol & Biol Program, Gainesville, FL 32611 USA. RP Wang, JP (reprint author), Univ Florida, Dept Agron, Gainesville, FL 32611 USA.; Wang, JP (reprint author), Fujian Agr & Forestry Univ, Haixia Inst Sci & Technol, Ctr Genom & Biotechnol, Fuzhou, Peoples R China.; Wang, JP (reprint author), Univ Florida, Genet Inst, Plant Mol & Biol Program, Gainesville, FL 32611 USA. EM wangjp@ufl.edu FU Office of Science (BER); Department of Energy FX This work was supported by the Office of Science (BER), Department of Energy. We would like to thank Dr. Xiao-Qin Xia for assisting with probe parameters calculation. We are grateful to James Maku and Dev Paudel at University of Florida on revising the manuscript. NR 81 TC 0 Z9 0 U1 11 U2 12 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JUN 8 PY 2016 VL 7 AR 804 DI 10.3389/fpls.2016.00804 PG 18 WC Plant Sciences SC Plant Sciences GA DN7LY UT WOS:000377258700001 PM 27375658 ER PT J AU Burrells, A Opsteegh, M Pollock, KG Alexander, CL Chatterton, J Evans, R Walker, R McKenzie, CA Hill, D Innes, EA Katzer, F AF Burrells, Alison Opsteegh, Marieke Pollock, Kevin G. Alexander, Claire L. Chatterton, Jean Evans, Roger Walker, Robert McKenzie, Chris-Anne Hill, Dolores Innes, Elisabeth A. Katzer, Frank TI The prevalence and genotypic analysis of Toxoplasma gondii from individuals in Scotland, 2006-2012 SO PARASITES & VECTORS LA English DT Article DE Toxoplasma gondii; Human brains; Blood donors; Serology; Longitudinal study; Seroprevalence; Seroreversion; Genotyping ID PREGNANT-WOMEN; INFECTION; AGE; IDENTIFICATION; POPULATION; DISEASE; UK; SEROEPIDEMIOLOGY; SEROPREVALENCE; MYOCARDITIS AB Background: Contemporary information relating to the prevalence of Toxoplasma gondii in humans is lacking for the UK population, with even less information available about the human prevalence of the parasite in Scotland. To address this, two different study groups were used to determine the prevalence and genotypes of Toxoplasma gondii in the Scottish population. Methods: The first study group included serum samples from blood donors (n = 3273) over a four-year period (2006-2009) and the second study group comprised of DNA samples extracted from human brains (n = 151) over a five-year period (2008-2012). A T. gondii IgG ELISA was performed to determine seroprevalence and available sera from individuals who had seroconverted were tested by TgERP ELISA (sporozoite specific antigen). Human brain DNA was tested for T. gondii by ITS1 PCR and positives genotyped at the SAG3 and GRA6 loci by PCR-RFLP analysis. Results: Seroprevalence to T. gondii from blood donors was found to be 13.2 % (95 % CI: 11.5-15.1 %). Evidence of seroconversion (n = 2) as well as reversion to sero-negative status (n = 6) was evident from blood donors who had donated within all four collection periods (n = 184). The TgERP ELISA (indicating oocyst infection) was positive for one individual. The molecular detection of T. gondii DNA from human brains indicated a prevalence of 17.9 % (95 % CI: 12.1-24.9 %), with genotyping identifying alleles for types I and III. An increase in age was associated with an increase in detection of the parasite within both study groups. Conclusions: Our research provides current figures for the prevalence of T. gondii in Scotland and also shows evidence of seroreversion within the cohort of blood donors. In both study groups there was a correlation between increasing age and an increase in T. gondii prevalence, indicating that acquired infection plays an important role within the Scottish population. C1 [Burrells, Alison; Innes, Elisabeth A.; Katzer, Frank] Moredun Res Inst, Pentlands Sci Pk, Penicuik, Midlothian, Scotland. [Opsteegh, Marieke] Natl Inst Publ Hlth & Environm RIVM, Ctr Infect Dis Control, Bilthoven, Netherlands. [Pollock, Kevin G.] Natl Serv Scotland, Hlth Protect Scotland, Glasgow, Lanark, Scotland. [Alexander, Claire L.] Glasgow Royal Infirm, Scottish Parasite Diagnost & Reference Lab, Glasgow G4 0SF, Lanark, Scotland. [Chatterton, Jean; Evans, Roger] Raigmore Hosp, Dept Microbiol, Scottish Toxoplasma Reference Lab, Inverness, Scotland. [Walker, Robert] Quintiles, Almondvale Way, Livingston, West Lothian, Scotland. [McKenzie, Chris-Anne] Univ Edinburgh, Sudden Death Brain Bank, Dept Pathol Neuropathol, Wilkie Bldg Teviot Pl, Edinburgh, Midlothian, Scotland. [Hill, Dolores] ARS, USDA, Northeast Area, Anim Parasit Dis Lab, Beltsville, MD USA. RP Katzer, F (reprint author), Moredun Res Inst, Pentlands Sci Pk, Penicuik, Midlothian, Scotland. EM frank.katzer@moredun.ac.uk FU Moredun Foundation; Scottish Government, Rural and Environmental Sciences and Analytical Services (RESAS); Marie Curie fellowship FX AB was funded by the Moredun Foundation. FK and EAI were supported by the Scottish Government, Rural and Environmental Sciences and Analytical Services (RESAS). MO received a Marie Curie fellowship (FP7-PEOPLE-2010-IEF). NR 39 TC 2 Z9 2 U1 1 U2 6 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD JUN 7 PY 2016 VL 9 AR 324 DI 10.1186/s13071-016-1610-6 PG 12 WC Parasitology SC Parasitology GA DN8OR UT WOS:000377339000001 PM 27267112 ER PT J AU Zuiderveen, GH Padder, BA Kamfwa, K Song, QJ Kelly, JD AF Zuiderveen, Grady H. Padder, Bilal A. Kamfwa, Kelvin Song, Qijian Kelly, James D. TI Genome-Wide Association Study of Anthracnose Resistance in Andean Beans (Phaseolus vulgaris) SO PLOS ONE LA English DT Article ID COLLETOTRICHUM-LINDEMUTHIANUM; DISEASE-RESISTANCE; CONDITIONING RESISTANCE; MOLECULAR MARKERS; GENES; LOCUS; RACES; IDENTIFICATION; ARABIDOPSIS; INHERITANCE AB Anthracnose is a seed-borne disease of common bean (Phaseolus vulgaris L.) caused by the fungus Colletotrichum lindemuthianum, and the pathogen is cosmopolitan in distribution. The objectives of this study were to identify new sources of anthracnose resistance in a diverse panel of 230 Andean beans comprised of multiple seed types and market classes from the Americas, Africa, and Europe, and explore the genetic basis of this resistance using genome-wide association mapping analysis (GWAS). Twenty-eight of the 230 lines tested were resistant to six out of the eight races screened, but only one cultivar Uyole98 was resistant to all eight races (7, 39, 55, 65, 73, 109, 2047, and 3481) included in the study. Outputs from the GWAS indicated major quantitative trait loci (QTL) for resistance on chromosomes, Pv01, Pv02, and Pv04 and two minor QTL on Pv10 and Pv11. Candidate genes associated with the significant SNPs were detected on all five chromosomes. An independent QTL study was conducted to confirm the physical location of the Co-1 locus identified on Pv01 in an F-4:6 recombinant inbred line (RIL) population. Resistance was determined to be conditioned by the single dominant gene Co-1 that mapped between 50.16 and 50.30 Mb on Pv01, and an InDel marker (NDSU_IND_1_50.2219) tightly linked to the gene was developed. The information reported will provide breeders with new and diverse sources of resistance and genomic regions to target in the development of anthrac-nose resistance in Andean beans. C1 [Zuiderveen, Grady H.; Padder, Bilal A.; Kamfwa, Kelvin; Kelly, James D.] Michigan State Univ, Dept Plant Soil & Microbial Sci, 1066 Bogue St, E Lansing, MI 48824 USA. [Song, Qijian] USDA ARS, Soybean Genom & Improvement Lab, BARC, 10300 Baltimore Ave, Beltsville, MD 20705 USA. [Zuiderveen, Grady H.] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [Padder, Bilal A.] Sher E Kashmir Univ Agr Sci & Technol Kashmir, Srinagar 191121, Jammu & Kashmir, India. [Kamfwa, Kelvin] Univ Zambia, Dept Plant Sci, Sch Agr Sci, POB 32379,Great East Rd, Lusaka, Zambia. RP Kelly, JD (reprint author), Michigan State Univ, Dept Plant Soil & Microbial Sci, 1066 Bogue St, E Lansing, MI 48824 USA. EM kellyj@msu.edu OI Padder, Bilal A/0000-0001-8842-2432 FU Feed the Future Innovation Lab for Collaborative Research on Grain Legumes by the Bureau for Economic Growth, Agriculture, and Trade, U.S. Agency for International Development [EDH-A-00-07-00005-00] FX Research was supported by the Feed the Future Innovation Lab for Collaborative Research on Grain Legumes by the Bureau for Economic Growth, Agriculture, and Trade, U.S. Agency for International Development, under the terms of Cooperative Agreement No. EDH-A-00-07-00005-00. The opinions expressed in this publication are those of the authors and do not necessarily reflect the views of the U.S. Agency for International Development or the U.S. Government. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 65 TC 1 Z9 1 U1 9 U2 19 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 6 PY 2016 VL 11 IS 6 AR e0156391 DI 10.1371/journal.pone.0156391 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DO1SU UT WOS:000377560200008 PM 27270627 ER PT J AU Lemire, KA Rodriguez, YY McIntosh, MT AF Lemire, Karissa A. Rodriguez, Yelitza Y. McIntosh, Michael T. TI Alkaline hydrolysis to remove potentially infectious viral RNA contaminants from DNA SO VIROLOGY JOURNAL LA English DT Article ID MOUTH-DISEASE VIRUS; SWINE-FEVER VIRUS; REVERSE-TRANSCRIPTASE; RAPID DETECTION; PCR ASSAY; RT-PCR; CELLS AB Background: Diagnostics and research of high-consequence animal disease agents is often limited to laboratories with a high level of biosecurity that restrict the transport of biological material. Often, sharing of DNA with external partners is needed to support diagnostics, forensics, or research. Even in the absence of virus, RNA from positive-sense single stranded RNA (+ssRNA) viruses that may contaminate otherwise purified DNA preparations continues to pose a threat due to its potential to be infectious via direct translation to yield viral proteins. While the risk of animal infection or accidental reconstitution and release of a virus from RNA is very low, the high impact of an animal disease event associated with the accidental release of some +ssRNA viruses, such as classical swine fever or foot-and-mouth disease viruses, necessitates the precaution of having procedures to ensure the complete inactivation of viruses and +ssRNA viral genomes. RNA and DNA are differentially susceptible to enzymatic degradations; however, such procedures are susceptible to unintended DNA damage and/or failure due to enzyme or cofactor instabilities. Therefore, we describe the development and verification of a robust and simple chemical and physical method to selectively degrade RNA from purified DNA preparations. The procedure employs incubation of DNA in 0.25 N sodium hydroxide at 65 degrees C for 1 h followed by neutralization and boiling for 10 min to hydrolyze contaminating RNA and inactivate animal disease viruses from DNA preparations. Additional critical quality control elements include use of a synthetic control RNA (SCR) and an SCR-specific real-time RT-PCR to track effectiveness of the procedure in a parallel treated control sample, and a pH check of reagents to ensure proper neutralization of alkaline conditions. Results: The new procedure reduced intact RNA beyond the limit of detection by realtime RT-PCR and inactivated viruses by in vitro culture infectivity assays. Conclusions: Treated DNA, while denatured, remains suitable for most common molecular biology procedures including PCR, transformation of E. coli, and molecular sequencing. The procedure ensures not only the inactivation of a variety of viruses but also the degradation through hydrolysis of potentially contaminating infectious + ssRNA viral genomes. C1 [Lemire, Karissa A.; Rodriguez, Yelitza Y.; McIntosh, Michael T.] USDA, Plum Isl Anim Dis Ctr, Foreign Anim Dis Diagnost Lab, NVSL,STAS,VS,APHIS, POB 848, Greenport, NY 11944 USA. RP McIntosh, MT (reprint author), USDA, Plum Isl Anim Dis Ctr, Foreign Anim Dis Diagnost Lab, NVSL,STAS,VS,APHIS, POB 848, Greenport, NY 11944 USA. EM michael.t.mcintosh@aphis.usda.gov FU USDA APHIS NVSL graduate training program FX Data on inactivation of viruses by boiling was kindly provided by the FADDL under direction of Dr. James A. House. This work was supported by a USDA APHIS NVSL graduate training program award to KAL. NR 28 TC 1 Z9 1 U1 1 U2 2 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1743-422X J9 VIROL J JI Virol. J. PD JUN 4 PY 2016 VL 13 AR 88 DI 10.1186/s12985-016-0552-0 PG 12 WC Virology SC Virology GA DN3QC UT WOS:000376976800001 PM 27260412 ER PT J AU Inskip, M Mavros, Y Sachdev, PS Singh, MAF AF Inskip, Michael Mavros, Yorgi Sachdev, Perminder S. Singh, Maria A. Fiatarone TI Exercise for Individuals with Lewy Body Dementia: A Systematic Review SO PLOS ONE LA English DT Review ID DISEASE RATING-SCALE; MINIMAL DETECTABLE CHANGE; PARKINSON-DISEASE; ALZHEIMERS-DISEASE; COGNITIVE IMPAIRMENT; BODIES; GAIT; PEOPLE; RELIABILITY; METAANALYSIS AB Background Individuals with Lewy body Dementia (LBD), which encompasses both Parkinson disease dementia (PDD) and Dementia with Lewy Bodies (DLB) experience functional decline through Parkinsonism and sedentariness exacerbated by motor, psychiatric and cognitive symptoms. Exercise may improve functional outcomes in Parkinson's disease (PD), and Alzheimer's disease (AD). However, the multi-domain nature of the LBD cluster of symptoms (physical, cognitive, psychiatric, autonomic) results in vulnerable individuals often being excluded from exercise studies evaluating physical function in PD or cognitive function in dementia to avoid confounding results. This review evaluated existing literature reporting the effects of exercise interventions or physical activity (PA) exposure on cluster symptoms in LBD. Methods A high-sensitivity search was executed across 19 databases. Full-length articles of any language and quality, published or unpublished, that analysed effects of isolated exercise/physical activity on indicative Dementia with Lewy Bodies or PD-dementia cohorts were evaluated for outcomes inclusive of physical, cognitive, psychiatric, physiological and quality of life measures. The protocol for this review (Reg. #:CRD42015019002) is accessible at http://www.crd.york.ac.uk/PROSPERO/. Results 111,485 articles were initially retrieved; 288 full articles were reviewed and 89.6% subsequently deemed ineligible due to exclusion of participants with co-existence of dementia and Parkinsonism. Five studies (1 uncontrolled trial, 1 randomized controlled trial and 3 case reports) evaluating 16 participants were included. Interventions were diverse and outcome homogeneity was low. Habitual gait speed outcomes were measured in 13 participants and increased (0.18m/s, 95% CI -0.02, 0.38m/s), exceeding moderate important change (0.14m/s) for PD cohorts. Other outcomes appeared to improve modestly in most participants. Discussion Scarce research investigating exercise in LBD exists. This review confirms exercise studies in PD and dementia consistently exclude LBD participants. Results in this cohort must be treated with caution until robustly designed, larger studies are commissioned to explore exercise efficacy, feasibility and clinical relevance. C1 [Inskip, Michael; Mavros, Yorgi; Singh, Maria A. Fiatarone] Univ Sydney, Exercise Hlth & Performance Fac Res Grp, Fac Hlth Sci, Lidcombe, NSW 2141, Australia. [Sachdev, Perminder S.] Univ New S Wales, Sch Psychiat, CHeBA Ctr Hlth Brain Ageing, Randwick, NSW 2031, Australia. [Sachdev, Perminder S.] Prince Wales Hosp, Neuropsychiat Inst, Randwick, NSW 2031, Australia. [Singh, Maria A. Fiatarone] Univ Sydney, Sydney Med Sch, Sydney, NSW 2006, Australia. [Singh, Maria A. Fiatarone] Hebrew SeniorLife, Roslindale, MA 02131 USA. [Singh, Maria A. Fiatarone] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr, Boston, MA 02155 USA. RP Inskip, M (reprint author), Univ Sydney, Exercise Hlth & Performance Fac Res Grp, Fac Hlth Sci, Lidcombe, NSW 2141, Australia. EM michael.inskip@sydney.edu.au OI Mavros, Yorgi/0000-0002-2588-0425 NR 44 TC 1 Z9 1 U1 9 U2 9 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 3 PY 2016 VL 11 IS 6 AR e0156520 DI 10.1371/journal.pone.0156520 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DN8ZV UT WOS:000377369700073 PM 27258533 ER PT J AU Jeong, JH Resop, JP Mueller, ND Fleisher, DH Yun, K Butler, EE Timlin, DJ Shim, KM Gerber, JS Reddy, VR Kim, SH AF Jeong, Jig Han Resop, Jonathan P. Mueller, Nathaniel D. Fleisher, David H. Yun, Kyungdahm Butler, Ethan E. Timlin, Dennis J. Shim, Kyo-Moon Gerber, James S. Reddy, Vangimalla R. Kim, Soo-Hyung TI Random Forests for Global and Regional Crop Yield Predictions SO PLOS ONE LA English DT Article ID CLIMATE-CHANGE; RANGE SHIFTS; CORN YIELDS; REGRESSION; CLASSIFICATION; TEMPERATURE; MODELS; RESPONSES; MACHINE; BIOMASS AB Accurate predictions of crop yield are critical for developing effective agricultural and food policies at the regional and global scales. We evaluated a machine-learning method, Random Forests (RF), for its ability to predict crop yield responses to climate and biophysical variables at global and regional scales in wheat, maize, and potato in comparison with multiple linear regressions (MLR) serving as a benchmark. We used crop yield data from various sources and regions for model training and testing: 1) gridded global wheat grain yield, 2) maize grain yield from US counties over thirty years, and 3) potato tuber and maize silage yield from the northeastern seaboard region. RF was found highly capable of predicting crop yields and outperformed MLR benchmarks in all performance statistics that were compared. For example, the root mean square errors (RMSE) ranged between 6 and 14% of the average observed yield with RF models in all test cases whereas these values ranged from 14% to 49% for MLR models. Our results show that RF is an effective and versatile machine-learning method for crop yield predictions at regional and global scales for its high accuracy and precision, ease of use, and utility in data analysis. RF may result in a loss of accuracy when predicting the extreme ends or responses beyond the boundaries of the training data. C1 [Jeong, Jig Han; Yun, Kyungdahm; Kim, Soo-Hyung] Univ Washington, Sch Environm & Forest Sci, Coll Environm, Box 354115, Seattle, WA 98195 USA. [Resop, Jonathan P.] Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. [Resop, Jonathan P.; Fleisher, David H.; Timlin, Dennis J.; Reddy, Vangimalla R.] USDA ARS, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. [Mueller, Nathaniel D.] Harvard Univ, Dept Earth & Planetary Sci, 20 Oxford St, Cambridge, MA 02138 USA. [Mueller, Nathaniel D.] Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA. [Butler, Ethan E.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Shim, Kyo-Moon] RDA, Climate Change & Agroecol Div, Natl Inst Agr Sci, Suwon, South Korea. [Gerber, James S.] Univ Minnesota, Inst Environm, St Paul, MN 55108 USA. RP Kim, SH (reprint author), Univ Washington, Sch Environm & Forest Sci, Coll Environm, Box 354115, Seattle, WA 98195 USA. EM soohkim@uw.edu RI Kim, Soo-Hyung/A-3012-2009 OI Kim, Soo-Hyung/0000-0003-3879-4080 FU Cooperative Research Program for Agricultural Science and Technology Development, Rural Development Administration, Republic of Korea [PJ01000707]; University of Washington [58-1265-1-074]; USDA-ARS [58-1265-1-074]; USDA-ARS Headquarters Postdoctoral Research Associate Program; USDA-NIFA-AFRI [2011-68004-30057]; USDA AFRI fellowship [2016-67012-25208]; NSF Hydrological Sciences grant [1521210]; Packard Foundation FX This study was supported by a Cooperative Research Program for Agricultural Science and Technology Development (Project No. PJ01000707), Rural Development Administration, Republic of Korea (SHK; KMS). Additional support was provided in part by a Specific Cooperative Agreement: 58-1265-1-074 between University of Washington and USDA-ARS (SHK; VRR), the USDA-ARS Headquarters Postdoctoral Research Associate Program (DHF), the USDA-NIFA-AFRI Grant no. 2011-68004-30057: Enhancing Food Security of Underserved Populations in the Northeast through Sustainable Regional Food Systems (DHF), the USDA AFRI fellowship 2016-67012-25208 (NDM), the NSF Hydrological Sciences grant 1521210 (NDM), and the Packard Foundation (EEB). NR 50 TC 0 Z9 0 U1 9 U2 9 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 3 PY 2016 VL 11 IS 6 AR e0156571 DI 10.1371/journal.pone.0156571 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DN8ZV UT WOS:000377369700078 PM 27257967 ER PT J AU Bock, CH Chen, CX Yu, FH Stevenson, KL Wood, BW AF Bock, Clive H. Chen, Chunxian Yu, Fahong Stevenson, Katherine L. Wood, Bruce W. TI Draft genome sequence of Fusicladium effusum, cause of pecan scab SO STANDARDS IN GENOMIC SCIENCES LA English DT Article DE Fusicladium effusum; Venturiacae; Fungal pathogen; Fungicide resistance; Genetic diversity; Pecan; Pecan scab ID CLADOSPORIUM-CARYIGENUM; DIVERSITY AB Pecan scab, caused by the plant pathogenic fungus Fusicladium effusum, is the most destructive disease of pecan, an important specialty crop cultivated in several regions of the world. Only a few members of the family Venturiaceae (in which the pathogen resides) have been reported sequenced. We report the first draft genome sequence (40.6 Mb) of an isolate F. effusum collected from a pecan tree (cv. Desirable) in central Georgia, in the US. The genome sequence described will be a useful resource for research of the biology and ecology of the pathogen, coevolution with the pecan host, characterization of genes of interest, and development of markers for studies of genetic diversity, genotyping and phylogenetic analysis. The annotation of the genome is described and a phylogenetic analysis is presented. C1 [Bock, Clive H.; Chen, Chunxian; Wood, Bruce W.] ARS, USDA, Southeastern Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. [Yu, Fahong] Univ Florida, Interdisciplinary Ctr Biotechnol Res, 2033 Mowry Rd, Gainesville, FL 32610 USA. [Stevenson, Katherine L.] Univ Georgia, Dept Plant Pathol, 2360 Rainwater Rd, Tifton, GA 31793 USA. RP Bock, CH (reprint author), ARS, USDA, Southeastern Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. EM clive.bock@ars.usda.gov FU USDA-ARS project [6042-21220-012-00] FX The research was supported through USDA-ARS project no. 6042-21220-012-00. The authors thank Dr. Mike Hotchkiss for help with sample collection, and Minling Zhang for technical support (USDA-ARS, Byron, GA). This article reports the results of research only. Mention of a trademark or proprietary product is solely for the purpose of providing specific information and does not constitute a guarantee or warranty of the product by the US Department of Agriculture and does not imply its approval to the exclusion of other products that may also be suitable. NR 38 TC 0 Z9 0 U1 7 U2 7 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1944-3277 J9 STAND GENOMIC SCI JI Stand. Genomic Sci. PD JUN 3 PY 2016 VL 11 AR 36 DI 10.1186/s40793-016-0161-y PG 7 WC Genetics & Heredity; Microbiology SC Genetics & Heredity; Microbiology GA DO7HI UT WOS:000377952800001 PM 27274782 ER PT J AU Wu, GY Peterson, AJ Morris, CF Murphy, KM AF Wu, Geyang Peterson, Adam J. Morris, Craig F. Murphy, Kevin M. TI Quinoa Seed Quality Response to Sodium Chloride and Sodium Sulfate Salinity SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE quinoa; soil salinity; protein content; hardness; density ID WILLD.; TOLERANCE; GROWTH; GERMINATION; EXPRESSION; VARIETIES; HARDNESS; TRAITS; SOIL AB Quinoa (Chenopodium quinoa Willd.) is an Andean crop with an edible seed that both contains high protein content and provides high quality protein with a balanced amino acid profile in embryonic tissues. Quinoa is a halophyte adapted to harsh environments with highly saline soil. In this study, four quinoa varieties were grown under six salinity treatments and two levels of fertilization, and then evaluated for quinoa seed quality characteristics, including protein content, seed hardness, and seed density. Concentrations of 8, 16, and 32 dS m(-1) of NaCl and Na2SO4, were applied to the soil medium across low (1 g N, 0.29 g P, 0.29 g K per pot) and high (3 g N, 0.85 g P, 0.86 g K per pot) fertilizer treatments. Seed protein content differed across soil salinity treatments, varieties, and fertilization levels. Protein content of quinoa grown under salinized soil ranged from 13.0 to 16.7%, comparable to that from non-saline conditions. NaCl and Na2SO4 exhibited different impacts on protein content. Whereas the different concentrations of NaCl did not show differential effects on protein content, the seed from 32 dS m(-1) Na2SO4 contained the highest protein content. Seed hardness differed among varieties, and was moderately influenced by salinity level (P = 0.09). Seed density was affected significantly by variety and Na2SO4 concentration, but was unaffected by NaCl concentration. The samples from 8 dS m-(1) Na2SO4 soil had lower density (0.66 g/cm(3)) than those from 16 dS m(-1) and 32 dS m(-1) Na2SO4, 0.74 and 0.72g/cm(3), respectively. This paper identifies changes in critical seed quality traits of quinoa as influenced by soil salinity and fertility, and offers insights into variety response and choice across different abiotic stresses in the field environment. C1 [Wu, Geyang] Washington State Univ, Sch Food Sci, Pullman, WA 99164 USA. [Peterson, Adam J.; Murphy, Kevin M.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Morris, Craig F.] Washington State Univ, ARS, USDA, Western Wheat Qual Lab, Pullman, WA 99164 USA. RP Murphy, KM (reprint author), Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. EM kmurphy2@wsu.edu FU USDA Organic Research and Extension initiative [NIFA:GRANT11083982] FX The research was funded by USDA Organic Research and Extension initiative,project number NIFA:GRANF11083982. NR 33 TC 1 Z9 1 U1 12 U2 20 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JUN 3 PY 2016 VL 7 AR 790 DI 10.3389/fpls.2016.00790 PG 8 WC Plant Sciences SC Plant Sciences GA DN3IY UT WOS:000376956800001 PM 27375648 ER PT J AU Xu, LS Jardini, TM Chen, WD AF Xu, Liangsheng Jardini, Teresa M. Chen, Weidong TI Direct repeat-mediated DNA deletion of the mating type MAT1-2 genes results in unidirectional mating type switching in Sclerotinia trifoliorum SO SCIENTIFIC REPORTS LA English DT Article ID UNITED-STATES; SELF-FERTILE; CERATOCYSTIS; HOMOEOCARPA; ASCOMYCETE AB The necrotrophic fungal pathogen Sclerotinia trifoliorum exhibits ascospore dimorphism and unidirectional mating type switching - self-fertile strains derived from large ascospores produce both self-fertile (large-spores) and self-sterile (small-spores) offsprings in a 4:4 ratio. The present study, comparing DNA sequences at MAT locus of both self-fertile and self-sterile strains, found four mating type genes (MAT1-1-1, MAT1-1-5, MAT1-2-1 and MAT1-2-4) in the self-fertile strain. However, a 2891-bp region including the entire MAT1-2-1 and MAT1-2-4 genes had been completely deleted from the MAT locus in the self-sterile strain. Meanwhile, two copies of a 146-bp direct repeat motif flanking the deleted region were found in the self-fertile strain, but only one copy of this 146-bp motif (a part of the MAT1-1-1 gene) was present in the self-sterile strain. The two direct repeats were believed to be responsible for the deletion through homologous intra-molecular recombination in meiosis. Tetrad analyses showed that all small ascospore-derived strains lacked the missing DNA between the two direct repeats that was found in all large ascospore-derived strains. In addition, heterokaryons at the MAT locus were observed in field isolates as well as in laboratory derived isolates. C1 [Xu, Liangsheng; Jardini, Teresa M.; Chen, Weidong] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Chen, Weidong] Washington State Univ, USDA ARS, Grain Legume Genet & Physiol Res Unit, Pullman, WA 99164 USA. RP Chen, WD (reprint author), Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA.; Chen, WD (reprint author), Washington State Univ, USDA ARS, Grain Legume Genet & Physiol Res Unit, Pullman, WA 99164 USA. EM w-chen@wsu.edu FU USDA ARS National Sclerotinia Initiative FX We thank two anonymous reviewers for critically evaluate an earlier version of the manuscript and helpful suggestions, and Dr. Daniel Mullendore from the Franceschi Microscopy and Imaging Center at Washington State University for technical support. The research was funded in part by the USDA ARS National Sclerotinia Initiative. NR 29 TC 0 Z9 0 U1 2 U2 2 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUN 3 PY 2016 VL 6 AR 27083 DI 10.1038/srep27083 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DN3YA UT WOS:000376999000001 PM 27255676 ER PT J AU Han, LJ Matarrita, J Sapozhnikova, Y Lehotay, SJ AF Han, Lijun Matarrita, Jessie Sapozhnikova, Yelena Lehotay, Steven J. TI Evaluation of a recent product to remove lipids and other matrix co-extractives in the analysis of pesticide residues and environmental contaminants in foods SO JOURNAL OF CHROMATOGRAPHY A LA English DT Article DE EMR-Lipid; Cleanup; QuEChERS; Sample preparation; Pesticide residue analysis; Fast GC-MS/MS; Analyte protectants; Environmental contaminants; Foods ID TANDEM MASS-SPECTROMETRY; PRESSURE GAS-CHROMATOGRAPHY; SOLID-PHASE EXTRACTION; QUECHERS SAMPLE PREPARATION; PERFORMANCE LIQUID-CHROMATOGRAPHY; MULTIRESIDUE ANALYSIS; LC-MS/MS; POLYCHLORINATED-BIPHENYLS; BIOLOGICAL MATRICES; FISH AB This study demonstrates the application of a novel lipid removal product to the residue analysis of 65 pesticides and 52 environmental contaminants in kale, pork, salmon, and avocado by fast, low pressure gas chromatography tandem mass spectrometry (LPGC-MS/MS). Sample preparation involves QuEChERS extraction followed by use of EMR-Lipid ("enhanced matrix removal of lipids") and an additional salting out step for cleanup. The optimal amount of EMR-Lipid was determined to be 500 mg for 2.5 mL extracts for most of the analytes. The co-extractive removal efficiency by the EMR-Lipid cleanup step was 83-98% for fatty samples and 79% for kale, including 76% removal of chlorophyll. Matrix effects were typically less than 20%, in part because analyte protectants were used in the LPGC MS/MS analysis. The recoveries of polycyclic aromatic hydrocarbons and diverse pesticides were mostly 70-120%, whereas recoveries of nonpolar polybrominated diphenyl ethers and polychlorinated biphenyls were mostly lower than 70% through the cleanup procedure. With the use of internal standards, method validation results showed that 76-85 of the 117 analytes achieved satisfactory results (recoveries of 70-120% and RSD <= 20%) in pork, avocado, and kale, while 53 analytes had satisfactory results in salmon. Detection limits were 5-10 ng/g for all but a few analytes. EMR-Lipid is a new sample preparation tool that serves as another useful option for cleanup in multiresidue analysis, particularly of fatty foods. Published by Elsevier B.V. C1 [Han, Lijun] China Agr Univ, Coll Sci, Beijing 100193, Peoples R China. [Han, Lijun; Matarrita, Jessie; Sapozhnikova, Yelena; Lehotay, Steven J.] ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Matarrita, Jessie] Univ Costa Rica, Ctr Invest Contaminac Ambiental, San Jose, Costa Rica. RP Lehotay, SJ (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM Steven.Lehotay@ars.usda.gov FU China Scholarship Council (CSC); Universidad de Costa Rica FX Technical assistance of Alan Lightfield and Tawana Simons is greatly appreciated. Lijun Han's sabbatical funding was provided by China Scholarship Council (CSC). Jessie Matarrita's funding was provided by the Universidad de Costa Rica. We also thank Derick Lucas for helpful discussions. NR 52 TC 2 Z9 2 U1 16 U2 42 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0021-9673 EI 1873-3778 J9 J CHROMATOGR A JI J. Chromatogr. A PD JUN 3 PY 2016 VL 1449 BP 17 EP 29 DI 10.1016/j.chroma.2016.04.052 PG 13 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA DM9OB UT WOS:000376694500004 PM 27139213 ER PT J AU Castell-Miller, CV Gutierrez-Gonzalez, JJ Tu, ZJ Bushley, KE Hainaut, M Henrissat, B Samac, DA AF Castell-Miller, Claudia V. Gutierrez-Gonzalez, Juan J. Tu, Zheng Jin Bushley, Kathryn E. Hainaut, Matthieu Henrissat, Bernard Samac, Deborah A. TI Genome Assembly of the Fungus Cochliobolus miyabeanus, and Transcriptome Analysis during Early Stages of Infection on American Wildrice (Zizania palustris L.) SO PLOS ONE LA English DT Article ID PLANT-PATHOGENIC FUNGUS; CYCLIC PEPTIDE BIOSYNTHESIS; POLYKETIDE VIRULENCE FACTOR; F-SP PLATANI; FUNCTIONAL-ANALYSIS; BROWN SPOT; T-TOXIN; HC-TOXIN; RNA-SEQ; CYTOCHROME-P450 MONOOXYGENASE AB The fungus Cochliobolus miyabeanus causes severe leaf spot disease on rice (Oryza sativa) and two North American specialty crops, American wildrice (Zizania palustris) and switchgrass (Panicum virgatum). Despite the importance of C. miyabeanus as a disease-causing agent in wildrice, little is known about either the mechanisms of pathogenicity or host defense responses. To start bridging these gaps, the genome of C. miyabeanus strain TG12bL2 was shotgun sequenced using Illumina technology. The genome assembly consists of 31.79 Mbp in 2,378 scaffolds with an N-50 = 74,921. It contains 11,000 predicted genes of which 94.5% were annotated. Approximately 10% of total gene number is expected to be secreted. The C. miyabeanus genome is rich in carbohydrate active enzymes, and harbors 187 small secreted peptides (SSPs) and some fungal effector homologs. Detoxification systems were represented by a variety of enzymes that could offer protection against plant defense compounds. The non-ribosomal peptide synthetases and polyketide synthases (PKS) present were common to other Cochliobolus species. Additionally, the fungal transcriptome was analyzed at 48 hours after inoculation in planta. A total of 10,674 genes were found to be expressed, some of which are known to be involved in pathogenicity or response to host defenses including hydrophobins, cutinase, cell wall degrading enzymes, enzymes related to reactive oxygen species scavenging, PKS, detoxification systems, SSPs, and a known fungal effector. This work will facilitate future research on C. miyabeanus pathogen-associated molecular patterns and effectors, and in the identification of their corresponding wildrice defense mechanisms. C1 [Castell-Miller, Claudia V.; Samac, Deborah A.] Univ Minnesota, Dept Plant Pathol, St Paul, MN USA. [Gutierrez-Gonzalez, Juan J.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN USA. [Gutierrez-Gonzalez, Juan J.; Samac, Deborah A.] USDA ARS Plant Sci Res Unit, St Paul, MN USA. [Tu, Zheng Jin] Mayo Clin, Div Biomed Stat & Informat, Rochester, MN USA. [Bushley, Kathryn E.] Univ Minnesota, Dept Plant Biol, St Paul, MN USA. [Hainaut, Matthieu; Henrissat, Bernard] Aix Marseille Univ, CNRS UMR 7257, Marseille, France. [Hainaut, Matthieu; Henrissat, Bernard] AFMB, USC 1408, INRA, Marseille, France. [Henrissat, Bernard] King Abdulaziz Univ, Dept Biol Sci, Jeddah 21413, Saudi Arabia. RP Castell-Miller, CV (reprint author), Univ Minnesota, Dept Plant Pathol, St Paul, MN USA. EM caste007@umn.edu RI Fac Sci, KAU, Biol Sci Dept/L-4228-2013; OI Gutierrez-Gonzalez, Juan/0000-0002-6795-6192 FU United States Department of Agriculture-Agricultural Research Service (ARS-USDA); Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231] FX Funding was provided by United States Department of Agriculture-Agricultural Research Service (ARS-USDA).r We would like to thank Bruce Elckloff from the Mayo Clinic, Rochester, MN for sequencing the C. miyabeanus isolate, and Dr. B. Gillian Turgeon and Bradford Condon for NRPS sequence data from the C. miyabeanus WK-1C and C. heterostrophus C4 and C5 strains, and other ascomycetes used in the analyses, and to Dr. B. Gillian Turgeon and the U.S. Department of Energy Joint Genome Institute (http://genome.jgi.doe.gov) for sharing the C. miyabeanus WK-1C DNA sequences and proteins. The genome and transcriptome of C. miyabeanus work were sequenced and annotated by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. NR 116 TC 0 Z9 0 U1 7 U2 22 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 2 PY 2016 VL 11 IS 6 AR e0154122 DI 10.1371/journal.pone.0154122 PG 31 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DN6XB UT WOS:000377218700002 PM 27253872 ER PT J AU Dukowic-Schulze, S Sundararajan, A Ramaraj, T Kianian, S Pawlowski, WP Mudge, J Chen, CB AF Dukowic-Schulze, Stefanie Sundararajan, Anitha Ramaraj, Thiruvarangan Kianian, Shahryar Pawlowski, Wojciech P. Mudge, Joann Chen, Changbin TI Novel Meiotic miRNAs and Indications for a Role of PhasiRNAs in Meiosis SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE meiosis; meiocytes; small RNA; phasiRNA; DNA methylation; maize; sRNA-seq; bisulfite sequencing ID FLORAL ORGAN IDENTITY; RICE SMALL RNAS; COMPREHENSIVE ANNOTATION; ARABIDOPSIS-THALIANA; FLOWER DEVELOPMENT; PLANT DEVELOPMENT; MICRORNA TARGETS; DNA METHYLATION; GENE; BIOGENESIS AB Small RNAs (sRNA) add additional layers to the regulation of gene expression, with siRNAs directing gene silencing at the DNA level by RdDM (RNA -directed DNA methylation), and micro RNAs (miRNAs) directing post -transcriptional regulation of specific target genes, mostly by mRNA cleavage. We used manually isolated male meiocytes from maize (Zea mays) to investigate sRNA and DNA methylation landscapes during zygotene, an early stage of meiosis during which steps of meiotic recombination and synapsis of paired homologous chromosomes take place. We discovered two novel miRNAs from meiocytes, zma-MIR11969 and zma-MIR11970, and identified putative target genes. Furthermore, we detected abundant phasiRNAs of 21 and 24 nt length. PhasiRNAs are phased small RNAs which occur in 21 or 24 nt intervals, at a few hundred loci, specifically in male reproductive tissues in grasses. So far, the function of phasiRNAs remained elusive. Data from isolated meiocytes now revealed elevated DNA methylation at phasiRNA loci, especially in the CHH context, suggesting a role for phasiRNAs in cis DNA methylation. In addition, we consider a role of these phasiRNAs in chromatin remodeling/dynamics during meiosis. However, this is not well supported yet and will need more additional data. Here, we only lay out the idea due to other relevant literature and our additional observation of a peculiar GC content pattern at phasiRNA loci. Chromatin remodeling is also indicated by the discovery that histone genes were enriched for sRNA of 22 nt length. Taken together, we gained clues that lead us to hypothesize sRNA-driven DNA methylation and possibly chromatin remodeling during male meiosis in the monocot maize which is in line with and extends previous knowledge. C1 [Dukowic-Schulze, Stefanie; Chen, Changbin] Univ Minnesota, Dept Hort Sci, St Paul, MN USA. [Sundararajan, Anitha; Ramaraj, Thiruvarangan; Mudge, Joann] Natl Ctr Genome Resources, Santa Fe, NM USA. [Kianian, Shahryar] ARS, Cereal Dis Lab, USDA, St Paul, MN USA. [Pawlowski, Wojciech P.] Cornell Univ, Sch Integrat Plant Sci, Sect Plant Biol, Ithaca, NY USA. RP Chen, CB (reprint author), Univ Minnesota, Dept Hort Sci, St Paul, MN USA. EM chenx481@umn.edu OI Dukowic-Schulze, Stefanie/0000-0003-2675-1075 FU National Science Foundation [1025881]; Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health [P20GM103451] FX This work was supported by the National Science Foundation (IOS: 1025881) and by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P20GM103451. NR 97 TC 2 Z9 2 U1 3 U2 16 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JUN 2 PY 2016 VL 7 AR 762 DI 10.3389/fpls.2016.00762 PG 18 WC Plant Sciences SC Plant Sciences GA DN1ZA UT WOS:000376862700001 PM 27313591 ER PT J AU Yang, J Hotz, T Broadnax, L Yarmarkovich, M Elbaz-Younes, I Hirschi, KD AF Yang, Jian Hotz, Tremearne Broadnax, LaCassidy Yarmarkovich, Mark Elbaz-Younes, Ismail Hirschi, Kendal D. TI Anomalous uptake and circulatory characteristics of the plant-based small RNA MIR2911 SO SCIENTIFIC REPORTS LA English DT Article ID EXOSOME-LIKE NANOPARTICLES; MICRORNAS; CELLS; DELIVERY; BIOAVAILABILITY; MICE AB Inconsistent detection of plant-based dietary small RNAs in circulation has thwarted the use of dietary RNA therapeutics. Here we demonstrate mice consuming diets rich in vegetables displayed enhanced serum levels of the plant specific small RNA MIR2911. Differential centrifugation, size-exclusion chromatography, and proteinase K treatment of plant extracts suggest this RNA resides within a proteinase K-sensitive complex. Plant derived MIR2911 was more bioavailable than the synthetic RNA. Furthermore, MIR2911 exhibited unusual digestive stability compared with other synthetic plant microRNAs. The characteristics of circulating MIR2911 were also unusual as it was not associated with exosomes and fractionated as a soluble complex that was insensitive to proteinase K treatment, consistent with MIR2911 being stabilized by modifications conferred by the host. These results indicate that intrinsic stability and plant-based modifications orchestrate consumer uptake of this anomalous plant based small RNA and invite revisiting plant-based microRNA therapeutic approaches. C1 [Yang, Jian; Broadnax, LaCassidy; Elbaz-Younes, Ismail; Hirschi, Kendal D.] USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, 1100 Bates St, Houston, TX 77030 USA. [Hotz, Tremearne] Bates Coll, 2 Andrews Rd, Lewiston, ME 04240 USA. [Yarmarkovich, Mark] Univ Penn, Canc Biol, 3501 Civ Ctr Blvd, Philadelphia, PA 19104 USA. [Hirschi, Kendal D.] Texas A&M Univ, Vegetable & Fruit Improvement Ctr, College Stn, TX 77845 USA. RP Hirschi, KD (reprint author), USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, 1100 Bates St, Houston, TX 77030 USA.; Hirschi, KD (reprint author), Texas A&M Univ, Vegetable & Fruit Improvement Ctr, College Stn, TX 77845 USA. EM kendalh@bcm.edu NR 23 TC 1 Z9 1 U1 6 U2 11 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUN 2 PY 2016 VL 6 AR 26834 DI 10.1038/srep26834 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DN1TZ UT WOS:000376849600001 PM 27251858 ER PT J AU Tworkoski, T Fazio, G Glenn, DM AF Tworkoski, Thomas Fazio, Gennaro Glenn, D. Michael TI Apple rootstock resistance to drought SO SCIENTIA HORTICULTURAE LA English DT Article DE Fruit tree; Hormone; Root system; Tree size; Water stress ID TANDEM MASS-SPECTROMETRY; ABSCISIC-ACID; TOLERANCE; TREES; PATHWAYS; GROWTH; PLANTS AB Water for irrigation will likely be less available in apple-growing regions due to climate change and competition with human needs other than agriculture. Apple cultivars and rootstocks may differ in water use necessary for acceptable cropping. In two greenhouse experiments in 2014 and 2015 rootstocks (M.9 and MM.111) and scions ('Gala' and 'Fuji') with known differences in size control and potential resistance to drought were compared under conditions of reduced water availability. After 1 week without irrigation in 2014 the potting soil moisture and leaf water potential (Psi(L)) were reduced more in trees on MM.111 than M.9 rootstocks and by 'Gala' than 'Fuji' scions. Abscisic acid (ABA) and associated metabolites dihydrophaseic acid, abscisic acid glucose ester, and phaseic acid generally were greater for both scions on M.9 than MM.111. Concentrations of ABA metabolites were greater in 'Gala' than 'Fuji' suggesting significant metabolic rates in leaves. As noon Psi(L) decreased between -1.5 and -2.0 MPa leaf ABA levels increased exponentially to concentrations above 500 ng/g dw and stomatal conductance (g(s)) decreased to less than 50 mmole H2O m(-2) s(-1). At reduced g(s) carbon assimilation (A) was low but greater in trees on M.9 than on MM.111. Trees grown on M.9 partitioned less dry weight (dw) to roots than MM.111. However the% dw partitioned to fine roots was greater in M.9 than MM.111 which may have helped trees grow in a small environment such as a pot. In 2015 trees were grown in 45- and 75-L pots and irrigated over 3 weeks to maintain a steady Psi(L). between -1.5 and -2.5 MPa. This longer-term water stress in 2015 resulted in greater g(s) and A in trees on MM.111 and in larger pots but WUE was still greater in trees grafted to M.9 in 3 of the 5 drought dates measured. Both rootstocks provided drought resistance, but by mechanisms which appear to differ and which may provide benefits to apple trees grown with reduced irrigation or with water stress. The dwarfing rootstock M.9 produces higher levels of ABA that may regulate stomatal opening and improve short-term drought resistance. The more invigorating rootstock MM.111 may be drought hardy in the longer-term due to development of a more extensive root system. Published by Elsevier B.V. C1 [Tworkoski, Thomas; Glenn, D. Michael] ARS, Appalachian Fruit Res Stn, USDA, Kearneysville, WV USA. [Fazio, Gennaro] Cornell Univ, Plant Genet Resources Unit, ARS, USDA, Geneva, NY USA. RP Tworkoski, T (reprint author), ARS, Appalachian Fruit Res Stn, USDA, Kearneysville, WV USA. EM tom.tworkoski@ars.usda.gov NR 30 TC 1 Z9 1 U1 12 U2 36 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD JUN 2 PY 2016 VL 204 BP 70 EP 78 DI 10.1016/j.scienta.2016.01.047 PG 9 WC Horticulture SC Agriculture GA DL8JG UT WOS:000375887700009 ER PT J AU Park, Y Ligaray, M Kim, YM Kim, JH Cho, KH Sthiannopkao, S AF Park, Yongeun Ligaray, Mayzonee Kim, Young Mo Kim, Joon Ha Cho, Kyung Hwa Sthiannopkao, Suthipong TI Development of enhanced groundwater arsenic prediction model using machine learning approaches in Southeast Asian countries SO DESALINATION AND WATER TREATMENT LA English DT Article DE Groundwater; Arsenic contamination; Machine learning; Support vector machine; Artificial neural network; Southeast Asian countries ID ARTIFICIAL NEURAL-NETWORK; WATER-RESOURCES; DRINKING-WATER; CONTAMINATION; PARAMETERS; REGRESSION; CAMBODIA; SEDIMENTS; MEKONG; INDIA AB Groundwater contamination with arsenic (As) is one of the major issues in the world, especially for Southeast Asian (SEA) countries where groundwater is the major drinking water source, especially in rural areas. Unfortunately, quantification of groundwater As contamination is another burden for those countries because it requires sophisticated equipment, expensive analysis, and well-trained technicians. Here, we collected approximately 350 groundwater samples from three different SEA countries, including Cambodia, Lao PDR, and Thailand, in an attempt to quantify total As concentrations and conventional water quality variables. After that, two machine learning models (i.e. artificial neural network (ANN) and support vector machine (SVM)) were applied to predict groundwater As contamination using conventional water quality parameters. Prior to modeling approaches, the pattern search algorithm in MATLAB software was used to optimize the ANN and SVM model parameters, attempting to find the best parameters set for modeling groundwater As concentrations. Overall, the SVM showed the superior prediction performance, giving higher Nash-Sutcliffe coefficients than ANN in both the training and validation periods. We hope that the model developed by this study could be a suitable quantification tool for groundwater As contamination in SEA countries. C1 [Park, Yongeun] USDA ARS, Environm Microbial & Food Safety Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. [Ligaray, Mayzonee; Cho, Kyung Hwa] Ulsan Natl Inst Sci & Technol, Sch Urban & Environm Engn, Ulsan 689798, South Korea. [Kim, Young Mo; Kim, Joon Ha] Gwangju Inst Sci & Technol, Sch Environm Sci & Engn, 261 Cheomdan Gwagiro, Gwangju 500712, South Korea. [Sthiannopkao, Suthipong] Dong A Univ, Dept Environm Engn, Busan 604714, South Korea. RP Cho, KH (reprint author), Ulsan Natl Inst Sci & Technol, Sch Urban & Environm Engn, Ulsan 689798, South Korea.; Sthiannopkao, S (reprint author), Dong A Univ, Dept Environm Engn, Busan 604714, South Korea. EM khcho@unist.ac.kr; suthisuthi@gmail.com OI Park, Yongeun/0000-0002-1959-0843 FU Toyota Foundation [D11-R-81]; National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2014R1A1A2059680] FX This study was funded by the Toyota Foundation, grant number D11-R-81. And this research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by theMinistry of Education (NRF-2014R1A1A2059680). NR 50 TC 0 Z9 0 U1 6 U2 31 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 1944-3994 EI 1944-3986 J9 DESALIN WATER TREAT JI Desalin. Water Treat. PD JUN 2 PY 2016 VL 57 IS 26 BP 12227 EP 12236 DI 10.1080/19443994.2015.1049411 PG 10 WC Engineering, Chemical; Water Resources SC Engineering; Water Resources GA DG0AH UT WOS:000371723600027 ER PT J AU Rossman, AY Allen, WC Castlebury, LA AF Rossman, Amy Y. Allen, W. Cavan Castlebury, Lisa A. TI New combinations of plant-associated fungi resulting from the change to one name for fungi SO IMA FUNGUS LA English DT Article DE Fungi; nomenclature; plant pathogens; pleomorphic fungi; scientific names ID GLOMERELLA-MAGNA; GENERA; RAMULARIA; GENUS; PLEOSPOROMYCETIDAE; MYTILINIDIACEAE; SEIMATOSPORIUM; RHYTISMATACEAE; HAPLOTRICHUM; HYSTERIACEAE AB In advancing to one scientific name for each fungus species, a number of name changes are required especially for plant-associated fungi. These include species names that are not in the correct genus. For example, the generic name Elsinoe is used for fungi causing scab diseases but a number of these species were described in the asexually typified genus Sphaceloma and must be placed in Elsinoe. In other cases species names were determined to be unrelated to the type species of the genus in which they are currently placed and are placed in a more appropriate genus. For each new name the history, rationale and importance of the name is discussed. The following new combinations are made: Acanthohelicospora aurea, A. scopula, Bifusella ahmadii, Botryobasidium capitatum, B. rubiginosum, Colletotrichum magnum, Crandallia acuminata, C. antarctica, Elsinoe arachadis, E. freyliniae, E. necator, E. perseae, E. poinsettiae, E. punicae, Entyloma gibbum, Harknessia farinosa, Passalora alocasiae, Protoventuria veronicae, Pseudocercosporella ranunculi, Psiloglonium stygium, Ramularia pseudomaculiformis, Seimatosporium tostum, Thielaviopsis radicicola combs. nov., and Venturia effusa. C1 [Rossman, Amy Y.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Allen, W. Cavan] North Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. [Allen, W. Cavan; Castlebury, Lisa A.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. RP Rossman, AY (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM amydianer@yahoo.com NR 55 TC 0 Z9 0 U1 1 U2 1 PU INT MYCOLOGICAL ASSOC PI BERKELEY PA C/O J TAYLOR, DEPT PLANT & MICROBIAL BIOLOGY, BERKELEY, CA 94720 USA SN 2210-6340 EI 2210-6359 J9 IMA FUNGUS JI IMA Fungus PD JUN PY 2016 VL 7 IS 1 BP 1 EP 7 DI 10.5598/imafungus.2016.07.01.01 PG 7 WC Mycology SC Mycology GA EJ7JB UT WOS:000393396700016 PM 27433437 ER PT J AU Castellano, MA Dentinger, BTM Sene, O Elliott, TF Truong, C Henkel, TW AF Castellano, Michael A. Dentinger, Bryn T. M. Sene, Olivier Elliott, Todd F. Truong, Camille Henkel, Terry W. TI New species of Elaphomyces (Elaphomycetaceae, Eurotiales, Ascomycota) from tropical rainforests of Cameroon and Guyana SO IMA FUNGUS LA English DT Article DE biogeography; ectomycorrhizal fungi; Gilbertiodendron; Guiana Shield; Guineo-Congolian rainforest; Pakaraimaea; sequestrate fungi; Uapaca ID ALIGNMENT; SEQUENCE; MAFFT; PSEUDOTULOSTOMA; IDENTIFICATION; FUNGI; DNA AB The sequestrate false truffles Elaphomyces favosus, E. iuppitercellus, and E. labyrinthinus spp. nov. are described as new to science from the Dja Biosphere Reserve, Cameroon. Elaphomyces adamizans sp. nov. is described as new from the Pakaraima Mountains of Guyana. The Cameroonian species are the first Elaphomyces taxa to be formally described from Africa, occurring in lowland Guineo-Congolian tropical rainforests dominated by the ectomycorrhizal (ECM) canopy tree Gilbertiodendron dewevrei (Fabaceae subfam. Caesalpinioideae). The Guyanese species is the third to be discovered in lowland tropical South America, occurring in forests dominated by the ECM trees Pakaraimaea dipterocarpacea (Dipterocarpaceae) and Dicymbe jenmanii (Fabaceae subfam. Caesalpinioideae). Macromorphological, micromorphological, habitat, and DNA sequence data are provided for each new species. Molecular and morphological data place these fungi in Elaphomycetaceae (Eurotiales, Ascomycota). Unique morphological features are congruent with molecular delimitation of each of the new species based on a phylogenetic analysis of the rDNA ITS and 28S loci across the Elaphomycetaceae. The phylogenetic analysis also suggests that a common ancestor is shared between some Elaphomyces species from Africa and South America, and that species of the stalked, volvate genus Pseudotulostoma may be nested in Elaphomyces. C1 [Castellano, Michael A.] USDA, Forest Serv, Northern Res Stn, 3200 Jefferson Way, Corvallis, OR 97331 USA. [Dentinger, Bryn T. M.] Royal Bot Gardens, Comparat Plant & Fungal Biol, Richmond TW9 3DS, Surrey, England. [Sene, Olivier] Natl Herbarium Cameroon, Inst Agr Res Dev, POB 1601, Yaounde, Cameroon. [Elliott, Todd F.] Warren Wilson Coll, Dept Integrat Studies, Asheville, NC 28815 USA. [Truong, Camille] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA. [Henkel, Terry W.] Humboldt State Univ, Dept Biol Sci, Arcata, CA 95521 USA. RP Henkel, TW (reprint author), Humboldt State Univ, Dept Biol Sci, Arcata, CA 95521 USA. EM Terry.Henkel@humboldt.edu FU National Geographic Society's Committee for Research and Exploration grant [9235-13]; National Science Foundation (NSF) [DEB-0918591, DEB-1556338]; Basler Stiftung fur Biologische Forschung FX We thank the following funding sources: The National Geographic Society's Committee for Research and Exploration grant 9235-13 and National Science Foundation (NSF) DEB-0918591 and DEB-1556338 to T.W.H, and a grant to C.T. from the Basler Stiftung fur Biologische Forschung. In Cameroon the Ministry of Research and Scientific Innovation issued research permits. Jean Michel Onana, Head of The National Herbarium of Cameroon (Institute of Agricultural Research for Development, IRAD), provided much logistical assistance. The Conservator of the Dja Biosphere Reserve, Mengamenya Goue Achille, and his staff greatly assisted the fieldwork in the Dja. Additional research permits were granted by the Guyana Environmental Protection Agency. Field assistance in Cameroon was provided by Alamane Gabriel (a. k. a. Sikiro), Abate Jackson, Mamane Jean-Pierre, and Mei Lin Chin, and in Guyana by Dillon Husbands, Christopher Andrew, Peter Joseph, Francino Edmund, and Luciano Edmund. Caitlin Winterbottom and Connor Adams assisted with scanning electron microscopy. Laura Martinez-Suz isolated the ECM G. dewevrei root tip and generated its fungal ITS sequence. James Trappe assisted with deriving the Latin species names. Two anonymous reviewers provided very helpful comments on an earlier version of the manuscript. This paper is number 213 in the Smithsonian Institution's Biological Diversity of the Guiana Shield Program publication series. NR 27 TC 3 Z9 3 U1 0 U2 0 PU INT MYCOLOGICAL ASSOC PI BERKELEY PA C/O J TAYLOR, DEPT PLANT & MICROBIAL BIOLOGY, BERKELEY, CA 94720 USA SN 2210-6340 EI 2210-6359 J9 IMA FUNGUS JI IMA Fungus PD JUN PY 2016 VL 7 IS 1 BP 59 EP 73 DI 10.5598/imafungus.2016.07.01.05 PG 15 WC Mycology SC Mycology GA EJ7JB UT WOS:000393396700020 PM 27433441 ER PT J AU Boyacioglu, O Sulakvelidze, A Sharma, M Goktepe, I AF Boyacioglu, O. Sulakvelidze, A. Sharma, M. Goktepe, I. TI Effect of a bacteriophage cocktail in combination with modified atmosphere packaging in controlling Listeria monocytogenes on fresh-cut spinach SO IRISH JOURNAL OF AGRICULTURAL AND FOOD RESEARCH LA English DT Article DE bacteriophage; biocontrol; L. monocytogenes; modified atmosphere packaging; spinach ID ESCHERICHIA-COLI O157-H7; SHREDDED ICEBERG LETTUCE; GROWTH; BIOCONTROL; CHLORINE; FOOD; VEGETABLES; SURVIVAL; EFFICACY AB A Listeria monocytogenes-specific bacteriophage cocktail was evaluated for its activity against a nalidixic acid-resistant L. monocytogenes (Lm-NalR) isolate on fresh-cut spinach stored under modified atmosphere packaging at various temperatures. Pieces (similar to 2 x 2 cm(2)) of fresh spinach inoculated with 4.5 log CFU/cm(2) Lm-NalR were sprayed with the phage cocktail (6.5 log plaque-forming units [PFU]/cm(2)) or a control. The samples were stored at 4 degrees C or 10 degrees C for up to 14 d in sealed packages filled with either atmospheric air (AA) or modified atmosphere (MA). At 4 degrees C under AA, the phages significantly (P = 0.05) lowered the Lm-NalR populations on spinach, compared to control-treated inoculated samples, by 1.12 and 1.51 log CFU/cm(2) after 1 and 14 d, respectively. At 4 degrees C under MA, Lm-NalR was significantly reduced by 1.95 log CFU/cm(2) compared to control leaves after both 1 and 14 d. At 10 degrees C under AA, the phages significantly reduced Lm-NalR by 1.50 and 2.51 log CFU/cm(2) after 1 and 14 d compared to the control. Again at 10 degrees C under MA, the phages significantly reduced Lm-NalR by 1.71 and 3.24 log CFU/cm(2) compared to control after 1 and 14 d, respectively. The results support the potential of lytic bacteriophages in effectively reducing populations of L. monocytogenes on fresh-cut leafy produce, under both AA and MA conditions. C1 [Boyacioglu, O.] Adnan Menderes Univ, Dept Food Engn, Fac Engn, TR-09010 Aydin, Turkey. [Sulakvelidze, A.] Intralytix Inc, Baltimore, MD 21202 USA. [Sharma, M.] ARS, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Goktepe, I.] Qatar Univ, Dept Biol & Environm Sci, Doha, Qatar. RP Boyacioglu, O (reprint author), Adnan Menderes Univ, Dept Food Engn, Fac Engn, TR-09010 Aydin, Turkey. EM oboyaci@adu.edu.tr FU National Institute of Food and Agriculture of the US Department of Agriculture [NCX-2007-03435] FX This study was supported by the National Institute of Food and Agriculture of the US Department of Agriculture (project #NCX-2007-03435). NR 24 TC 0 Z9 0 U1 4 U2 4 PU TEAGASC PI CARLOW PA OAK PARK, CARLOW 00000, IRELAND SN 0791-6833 J9 IRISH J AGR FOOD RES JI Irish J. Agr. Food Res. PD JUN PY 2016 VL 55 IS 1 BP 74 EP 79 DI 10.1515/ijafr-2016-0007 PG 6 WC Agriculture, Multidisciplinary; Food Science & Technology SC Agriculture; Food Science & Technology GA EK0FL UT WOS:000393602000007 ER PT J AU Wolfenbarger, SN Massie, ST Ocamb, C Eck, EB Grove, GG Nelson, ME Probst, C Twomey, MC Gent, DH AF Wolfenbarger, Sierra N. Massie, Stephen T. Ocamb, Cynthia Eck, Emily B. Grove, Gary G. Nelson, Mark E. Probst, Claudia Twomey, Megan C. Gent, David H. TI Distribution and Characterization of Podosphaera macularis Virulent on Hop Cultivars Possessing R6-Based Resistance to Powdery Mildew SO PLANT DISEASE LA English DT Article ID DISEASE RESISTANCE; DOWNY MILDEW; FLAX RUST; GENES; SUSCEPTIBILITY; IDENTIFICATION; GENETICS; PLANTS; PATHOGENS; SEVERITY AB Host resistance, both quantitative and qualitative, is the preferred long-term approach for disease management in many pathosystems, including powdery mildew of hop (Podosphaera macularis). In 2012, an epidemic of powdery mildew occurred in Washington and Idaho on previously resistant cultivars whose resistance was putatively based on the gene designated R6. In 2013, isolates capable of causing severe disease on cultivars with R6-based resistance were confirmed in Oregon and became widespread during 2014. Surveys of commercial hop yards during 2012 to 2014 documented that powdery mildew is now widespread on cultivars possessing R6 resistance in Washington and Oregon, and the incidence of disease is progressively increasing. Pathogenic fitness, race, and mating type of Rd-virulent isolates were compared with isolates of P. macularis lacking R6 virulence. All isolates were positive for the mating type idiomorph MAT1-1 and were able to overcome resistance genes Rb, R3, and R5 but not 121 or R2. In addition, R6-virulent isolates were shown to infect differential cultivars reported to possess the R6 gene and also the R4 gene, although R4 has not yet been broadly deployed in the United States. R6-virulent isolates were not detected from the eastern United States during 2012 to 2015. In growth chamber studies, Rd-virulent isolates of P. macularis had a significantly longer latent period and produced fewer lesions on plants with R6 as compared with plants lacking R6, indicating a fitness cost to the fungus. R6-virulent isolates also produced fewer conidia when compared with isolates lacking R6 virulence, independent of whether the isolates were grown on a plant with or without R6. Thus, it is possible that the fitness cost of R6 virulence occurs regardless of host genotype. In field studies, powdery mildew was suppressed by at least 50% on plants possessing R6 as compared with those without R6 when coinoculated with R6-virulent and avirulent isolates. R6 virulence in P. macularis appears to be race specific and, at this time, imposes a measurable fitness penalty on the fungus. Resistance genes R1 and R2 appear to remain effective against Rd-virulent isolates of P. macularis in the U.S. Pacific Northwest. C1 [Wolfenbarger, Sierra N.; Ocamb, Cynthia; Eck, Emily B.; Twomey, Megan C.; Gent, David H.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Massie, Stephen T.; Gent, David H.] USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. [Grove, Gary G.; Nelson, Mark E.; Probst, Claudia] Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. RP Gent, DH (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA.; Gent, DH (reprint author), USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. EM dave.gent@ars.usda.gov FU United States Department of Agriculture Agricultural Research Service CRIS [2072-21000-046-00]; Hop Research Council; Washington Hop Commission; Washington State Commission on Pesticide Registrations; Western IPM Center [SA7762] FX Financial support was provided by the United States Department of Agriculture Agricultural Research Service CRIS 2072-21000-046-00, the Hop Research Council, Washington Hop Commission, Washington State Commission on Pesticide Registrations, and Western IPM Center (grant agreement number SA7762). We thank D. Gadoury for his suggestions and comments that improved this article; and the many people who provided powdery mildew samples for this project: D. Gadoury, J. Ravin, D. Higgins, R. Jeske, R. Kaitany, C. Rowher, E. Seigner, and M.-J. Welser. NR 47 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2016 VL 100 IS 6 BP 1212 EP 1221 DI 10.1094/PDIS-12-15-1449-RE PG 10 WC Plant Sciences SC Plant Sciences GA DL4UV UT WOS:000375634200023 ER PT J AU Wolfenbarger, SN Quesada-Ocampo, LM Gent, DH AF Wolfenbarger, S. N. Quesada-Ocampo, L. M. Gent, D. H. TI Powdery Mildew Caused by Podosphaera macularis on Hop (Humulus lupulus) in North Carolina SO PLANT DISEASE LA English DT News Item C1 [Wolfenbarger, S. N.; Gent, D. H.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Quesada-Ocampo, L. M.] N Carolina State Univ, Dept Plant Pathol, Box 7616, Raleigh, NC 27695 USA. [Gent, D. H.] USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. RP Wolfenbarger, SN (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. OI Quesada-Ocampo, Lina/0000-0002-9072-7531 NR 3 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2016 VL 100 IS 6 BP 1245 EP 1246 DI 10.1094/PDIS-12-15-1525-PDN PG 2 WC Plant Sciences SC Plant Sciences GA DL4UV UT WOS:000375634200061 ER PT J AU Sharon, JA Hathwaik, LT Glenn, GM Imam, SH Lee, CC AF Sharon, J. A. Hathwaik, L. T. Glenn, G. M. Imam, S. H. Lee, C. C. TI Isolation of efficient phosphate solubilizing bacteria capable of enhancing tomato plant growth SO JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION LA English DT Article DE Biofertilizer; Pantoea; phosphate solubilization; tomato growth stimulation ID IN-VITRO; RHIZOSPHERE; PROMOTION; PSEUDOMONAS; PHOSPHORUS; SOIL; TOLERANT; MAIZE AB Phosphorus is one of the three macronutrients that are essential for plant growth and development. Inorganic phosphorus (P), which can make up to 70% of the total P content in soils, can exist in calcium-, aluminum-, or iron-complexed forms that are unavailable for plant use. As a result, mineral phosphorus, P2O5, is often used as a fertilizer to supplement the nutrient for crop growth. To reduce the addition of mineral phosphorus to agricultural soils, research in naturally occurring phosphate-solubilizing microorganisms has been conducted for decades. This study found bacteria that solubilized phosphate at very high rates. The most efficient of the bacteria presented in this paper, Pantoea sp. Pot1, can solubilize tricalcium phosphate (Ca-3(PO4)(2)) at a rate of 956 mg L-1. This bacteria produces a variety of organic acids, including acetic, gluconic, formic, and propionic acids. Greenhouse experiments demonstrated that tomato plants with soil systems inoculated with Pantoea sp. Pot1 incorporated more P and produced much higher biomass weights than those plants without any added bacteria. C1 [Sharon, J. A.; Hathwaik, L. T.; Glenn, G. M.; Lee, C. C.] ARS, USDA, WRRC, 800 Buchanan St, Albany, CA 94710 USA. [Imam, S. H.] Flozyme Corp Inc, POB 260003, Plano, TX 75026 USA. RP Lee, CC (reprint author), ARS, USDA, WRRC, 800 Buchanan St, Albany, CA 94710 USA. EM Charles.Lee@ars.usda.gov FU United States Department of Agriculture [CRIS 2030-41000-058-00]; California Department of Food and Agriculture [SCB13051] FX We thank the Karl Linn Community Garden in Berkeley, California, USA for access to collect plant samples. This work was supported by the United States Department of Agriculture CRIS 2030-41000-058-00 and a grant from the California Department of Food and Agriculture (# SCB13051). The mention of firm names or trade products does not imply that they are endorsed or recommended by the US Department of Agriculture over other firms or similar products not mentioned. USDA is an equal opportunity provider and employer. NR 32 TC 0 Z9 0 U1 4 U2 4 PU SOC CHILENA CIENCIA SUELO PI TEMUCO PA CASILLA 54-D, TEMUCO, 00000, CHILE SN 0718-9516 J9 J SOIL SCI PLANT NUT JI J. Soil Sci. Plant Nutr. PD JUN PY 2016 VL 16 IS 2 BP 525 EP 536 PG 12 WC Soil Science SC Agriculture GA EH0DV UT WOS:000391433900018 ER PT J AU Kennedy, E Guthrie, JF AF Kennedy, Eileen Guthrie, Joanne F. TI Nutrition Assistance Programs: Cause or Solution to Obesity SO CURRENT OBESITY REPORTS LA English DT Article DE SNAP; WIC; NSLP; Evaluation; Obesity ID BIRTH-WEIGHT; FOOD STAMPS; PARTICIPATION; PREGNANCY; OUTCOMES; CHILDREN AB Three nutrition assistance programs-Supplemental Nutrition Assistance Program (SNAP), Special Nutrition Program for Women, Infants and Children (WIC), and National School Lunch Program (NSLP)-serve as the backbone of the nutrition safety net in the USA. These programs have been successful in achieving many of their initial goals of improving food purchases, food intake, and/or nutritional status of low-income, vulnerable Americans. The emphasis in these programs has now broadened to also include an obesity prevention focus. Recent changes in program components demonstrate the revised objectives of the program. SNAP, WIC, and NSLP increase economic access to an adequate diet but access alone is unlikely to be the total solution to obesity prevention. An ecological approach, incorporating the nutrition programs, appears to be a more promising strategy to leverage the impact of SNAP, WIC, and NSLP. C1 [Kennedy, Eileen] Tufts Univ, Friedman Sch Nutr Sci & Policy, 150 Harrison Ave, Sanriku, Iwate 02201, Japan. [Guthrie, Joanne F.] USDA, Econ Res Serv, 1400 Independence Ave SW,Mailstop 1800, Washington, DC 20520 USA. RP Kennedy, E (reprint author), Tufts Univ, Friedman Sch Nutr Sci & Policy, 150 Harrison Ave, Sanriku, Iwate 02201, Japan. EM eileen.kennedy@tufts.edu; Joanneguthrie66@gmail.com NR 52 TC 1 Z9 1 U1 7 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 2162-4968 J9 CURR OBES REP JI Curr. Obes. Rep. PD JUN PY 2016 VL 5 IS 2 BP 176 EP 183 DI 10.1007/s13679-016-0207-x PG 8 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA EB0ST UT WOS:000387056600004 PM 27033876 ER PT J AU Weerasekara, YK Roberts, SB Kahn, MA LaVertu, AE Hoffman, B Das, SK AF Weerasekara, Yasoma Kumari Roberts, Susan B. Kahn, Mira A. LaVertu, Amy E. Hoffman, Ben Das, Sai Krupa TI Effectiveness of Workplace Weight Management Interventions: a Systematic Review SO CURRENT OBESITY REPORTS LA English DT Article DE Weight management; Obesity; Overweight; Weight loss; Randomized trials ID RANDOMIZED CONTROLLED-TRIAL; CARDIOMETABOLIC RISK-FACTORS; BODY-WEIGHT; FINANCIAL INCENTIVES; WORKSITE NUTRITION; PHYSICAL-ACTIVITY; OBESITY; OVERWEIGHT; HEALTH; EMPLOYEES AB A systematic review was conducted of randomized trials of workplace weight management interventions, including trials with dietary, physical activity, environmental, behavioral, and incentive-based components. Main outcomes were defined as change in weight-related measures. Keywords related to weight management and workplace interventions were used to search relevant databases, and 23 eligible studies were reviewed in detail using a data extraction form and quality assessment checklist. The trials were conducted mainly in the USA and Europe, with four additional countries represented. Interventions were mostly multicomponent and were implemented in both sexes and in a range of employment categories. Intervention effectiveness appeared unrelated to region of the world and was highest in 6-12-month trials. The results ranged widely from clinically significant 8.8-kg weight loss in one trial to less effective than the control treatment in others. Some workplace interventions achieve clinically significant benefits, and further studies are needed to replicate those results in wider sociocultural and geographical contexts. C1 [Weerasekara, Yasoma Kumari; Roberts, Susan B.; Kahn, Mira A.; Das, Sai Krupa] Tufts Univ, Obes & Energet Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. [LaVertu, Amy E.] Tufts Univ, Hirsh Hlth Sci Lib, Boston, MA 02111 USA. [Hoffman, Ben] GE Co, Gen Elect Med Serv, Chicago, IL USA. [Hoffman, Ben] Univ Texas Sch Publ Hlth, San Antonio, TX USA. RP Das, SK (reprint author), Tufts Univ, Obes & Energet Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM Sai.Das@tufts.edu NR 46 TC 1 Z9 1 U1 7 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 2162-4968 J9 CURR OBES REP JI Curr. Obes. Rep. PD JUN PY 2016 VL 5 IS 2 BP 298 EP 306 DI 10.1007/s13679-016-0205-z PG 9 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA EB0ST UT WOS:000387056600016 PM 27023071 ER PT J AU Gilbert, MJ Miller, WG Yee, E Zomer, AL van der Graaf-van Bloois, L Fitzgerald, C Forbes, KJ Meric, G Sheppard, SK Wagenaar, JA Duim, B AF Gilbert, Maarten J. Miller, William G. Yee, Emma Zomer, Aldert L. van der Graaf-van Bloois, Linda Fitzgerald, Collette Forbes, Ken J. Meric, Guillaume Sheppard, Samuel K. Wagenaar, Jaap A. Duim, Birgitta TI Comparative Genomics of Campylobacter fetus from Reptiles and Mammals Reveals Divergent Evolution in Host-Associated Lineages SO GENOME BIOLOGY AND EVOLUTION LA English DT Article DE campylobacter fetus; reptile; mammal; comparative genomics; recombination; evolution ID MOLECULAR CHARACTERIZATION; SAP LOCUS; SEQUENCE; JEJUNI; STRAINS; RECOMBINATION; INFECTIONS; ANNOTATION; PREDICTION; PATHOGEN AB Campylobacter fetus currently comprises three recognized subspecies, which display distinct host association. Campylobacter fetus subsp. fetus and C. fetus subsp. venerealis are both associated with endothermic mammals, primarily ruminants, whereas C. fetus subsp. testudinum is primarily associated with ectothermic reptiles. Both C. fetus subsp. testudinum and C. fetus subsp. fetus have been associated with severe infections, often with a systemic component, in immunocompromised humans. To study the genetic factors associated with the distinct host dichotomy in C. fetus, whole-genome sequencing and comparison of mammal-and reptile associated C. fetus was performed. The genomes of C. fetus subsp. testudinum isolated from either reptiles or humans were compared with elucidate the genetic factors associated with pathogenicity in humans. Genomic comparisons showed conservation of gene content and organization among C. fetus subspecies, but a clear distinction between mammal-and reptile-associated C. fetus was observed. Several genomic regions appeared to be subspecies specific, including a putative tricarballylate catabolism pathway, exclusively present in C. fetus subsp. testudinum strains. Within C. fetus subsp. testudinum, sapA, sapB, and sapAB type strains were observed. The recombinant locus iamABC(mlaFED) was exclusively associated with invasive C. fetus subsp. testudinum strains isolated from humans. A phylogenetic reconstruction was consistent with divergent evolution in host-associated strains and the existence of a barrier to lateral gene transfer between mammal- and reptile-associated C. fetus. Overall, this study shows that reptile-associated C. fetus subsp. testudinum is genetically divergent from mammal- associated C. fetus subspecies. C1 [Gilbert, Maarten J.; Zomer, Aldert L.; van der Graaf-van Bloois, Linda; Wagenaar, Jaap A.; Duim, Birgitta] Univ Utrecht, Fac Vet Med, Dept Infect Dis & Immunol, NL-3508 TC Utrecht, Netherlands. [Miller, William G.; Yee, Emma] ARS, USDA, Produce Safety & Microbiol Res Unit, Albany, CA USA. [Zomer, Aldert L.; van der Graaf-van Bloois, Linda; Wagenaar, Jaap A.; Duim, Birgitta] WHO Collaborating Ctr Campylobacter, OIE Reference Lab Campylobacteriosis, Utrecht, Netherlands. [Fitzgerald, Collette] CDC, Biotechnol Core Facil Branch, Div Sci Resources, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA. [Forbes, Ken J.] Univ Aberdeen, Sch Med & Dent, Aberdeen AB9 1FX, Scotland. [Meric, Guillaume; Sheppard, Samuel K.] Swansea Univ, Inst Life Sci, Coll Med, Swansea, W Glam, Wales. [Sheppard, Samuel K.] Swansea Univ, MRC Cloud Based Infrastruct Microbial Bioinformat, Swansea, W Glam, Wales. [Sheppard, Samuel K.] Univ Oxford, Dept Zool, Oxford OX1 2JD, England. [Wagenaar, Jaap A.] Cent Vet Inst Wageningen UR, Lelystad, Netherlands. RP Duim, B (reprint author), Univ Utrecht, Fac Vet Med, Dept Infect Dis & Immunol, NL-3508 TC Utrecht, Netherlands.; Duim, B (reprint author), WHO Collaborating Ctr Campylobacter, OIE Reference Lab Campylobacteriosis, Utrecht, Netherlands. EM b.duim@uu.nl NR 52 TC 3 Z9 3 U1 2 U2 2 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1759-6653 J9 GENOME BIOL EVOL JI Genome Biol. Evol. PD JUN PY 2016 VL 8 IS 6 BP 2006 EP 2019 DI 10.1093/gbe/evw146 PG 14 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA EA1QZ UT WOS:000386368200012 PM 27333878 ER PT J AU Crous, PW Wingfield, MJ Richardson, DM Le Roux, JJ Strasberg, D Edwards, J Roets, F Hubka, V Taylor, PWJ Heykoop, M Martin, MP Moreno, G Sutton, DA Wiederhold, NP Barnes, CW Carlavilla, JR Gene, J Giraldo, A Guarnaccia, V Guarro, J Hernandez-Restrepo, M Kolarik, M Manjon, JL Pascoe, IG Popov, ES Sandoval-Denis, M Woudenberg, JHC Acharya, K Alexandrova, AV Alvarado, P Barbosa, RN Baseia, IG Blanchette, RA Boekhout, T Burgess, TI Cano-Lira, JF Cmokova, A Dimitrov, RA Dyakov, MY Duenas, M Dutta, AK Esteve-Raventos, F Fedosova, AG Fournier, J Gamboa, P Gouliamova, DE Grebenc, T Groenewald, M Hanse, B Hardy, GESJ Held, BW Jurjevic, Z Kaewgrajang, T Latha, KPD Lombard, L Luangsa-ard, JJ Lyskova, P Mallatova, N Manimohan, P Miller, AN Mirabolfathy, M Morozova, OV Obodai, M Oliveira, NT Ordonez, ME Otto, EC Paloi, S Peterson, SW Phosri, C Roux, J Salazar, WA Sanchez, A Sarria, GA Shin, HD Silva, BDB Silva, GA Smith, MT Souza-Motta, CM Stchigel, AM Stoilova-Disheva, MM Sulzbacher, MA Telleria, MT Toapanta, C Traba, JM Valenzuela-Lopez, N Watling, R Groenewald, JZ AF Crous, P. W. Wingfield, M. J. Richardson, D. M. Le Roux, J. J. Strasberg, D. Edwards, J. Roets, F. Hubka, V. Taylor, P. W. J. Heykoop, M. Martin, M. P. Moreno, G. Sutton, D. A. Wiederhold, N. P. Barnes, C. W. Carlavilla, J. R. Gene, J. Giraldo, A. Guarnaccia, V. Guarro, J. Hernandez-Restrepo, M. Kolarik, M. Manjon, J. L. Pascoe, I. G. Popov, E. S. Sandoval-Denis, M. Woudenberg, J. H. C. Acharya, K. Alexandrova, A. V. Alvarado, P. Barbosa, R. N. Baseia, I. G. Blanchette, R. A. Boekhout, T. Burgess, T. I. Cano-Lira, J. F. Cmokova, A. Dimitrov, R. A. Dyakov, M. Yu. Duenas, M. Dutta, A. K. Esteve-Raventos, F. Fedosova, A. G. Fournier, J. Gamboa, P. Gouliamova, D. E. Grebenc, T. Groenewald, M. Hanse, B. Hardy, G. E. St. J. Held, B. W. Jurjevic, Z. Kaewgrajang, T. Latha, K. P. D. Lombard, L. Luangsa-ard, J. J. Lyskova, P. Mallatova, N. Manimohan, P. Miller, A. N. Mirabolfathy, M. Morozova, O. V. Obodai, M. Oliveira, N. T. Ordonez, M. E. Otto, E. C. Paloi, S. Peterson, S. W. Phosri, C. Roux, J. Salazar, W. A. Sanchez, A. Sarria, G. A. Shin, H. -D. Silva, B. D. B. Silva, G. A. Smith, M. Th. Souza-Motta, C. M. Stchigel, A. M. Stoilova-Disheva, M. M. Sulzbacher, M. A. Telleria, M. T. Toapanta, C. Traba, J. M. Valenzuela-Lopez, N. Watling, R. Groenewald, J. Z. TI Fungal Planet description sheets: 400-468 SO PERSOONIA LA English DT Article DE ITS DNA barcodes; LSU; novel fungal species; systematics ID SP-NOV; PHYLOGENETIC CONTRIBUTIONS; MOLECULAR PHYLOGENY; SPECIES CONCEPT; LSU SEQUENCES; CHAENOTHECOPSIS; GENERA; GENUS; TAXONOMY; CRINIPELLIS AB Novel species of fungi described in the present study include the following from Australia: Vermiculariopsiella eucalypti, Mulderomyces natalis (incl. Mulderomycesgen. nov.), Fusicladium paraamoenum, Neotrimmatostroma paraexcentricum, and Pseudophloeospora eucalyptorum on leaves of Eucalyptus spp., Anungitea grevilleae (on leaves of Grevillea sp.), Pyrenochaeta acaciae (on leaves of Acacia sp.), and Brunneocarpos banksiae (incl. Brunneocarpos gen. nov.) on cones of Banksia attenuata. Novel foliicolous taxa from South Africa include Neosulcatispora strelitziae (on Strelitzia nicolai), Colletotrichum ledebouriae (on Ledebouria floridunda), Cylindrosympodioides brabejum (incl. Cylindrosympodioides gen. nov.) on Brabejum stellatifolium, Sclerostagonospora ericae (on Erica sp.), Setophoma cyperi (on Cyperus sphaerocephala), and Phaeosphaeria breonadiae (on Breonadia microcephala). Novelties described from Robben Island (South Africa) include Wojnowiciella cissampeli and Diaporthe cissampeli (both on Cissampelos capensis), Phaeotheca salicorniae (on Salicornia meyeriana), Paracylindrocarpon aloicola (incl. Paracylindrocarpon gen. nov.) on Aloe sp., and Libertasomyces myopori (incl. Libertasomyces gen. nov.) on Myoporum serratum. Several novelties are recorded from La Reunion (France), namely Phaeosphaeri-opsis agapanthi (on Agapanthus sp.), Roussoella solani (on Solanum mauritianum), Vermiculariopsiella acaciae (on Acacia heterophylla), Dothiorella acacicola (on Acacia mearnsii), Chalara clidemiae (on Clidemia hirta), Cytospora tibouchinae (on Tibouchina semidecandra), Diaporthe ocoteae (on Ocotea obtusata), Castanediella eucalypticola, Phaeophleospora eucalypticola and Fusicladium eucalypticola (on Eucalyptus robusta), Lareunionomyces syzygii (incl. Lareunionomyces gen. nov.) and Parawiesneriomyces syzygii (incl. Parawiesneriomyces gen. nov.) on leaves of Syzygium jambos. Novel taxa from the USA include Meristemomyces arctostaphylos (on Arctostaphylos patula), Ochroconis dracaenae (on Dracaena reflexa), Rasamsonia columbiensis (air of a hotel conference room), Paecilomyces tabacinus (on Nicotiana tabacum), Toxicocladosporium hominis (from human broncoalveolar lavage fluid), Nothophoma macrospora (from respiratory secretion of a patient with pneumonia), and Penidiellopsis radicularis (incl. Penidiellopsis gen. nov.) from a human nail. Novel taxa described from Malaysia include Prosopidicola albizziae (on Albizzia falcataria), Proxipyricularia asari (on Asarum sp.), Diaporthe passifloricola (on Passiflora foetida), Paramycoleptodiscus albizziae (incl. Paramycoleptodiscus gen. nov.) on Albizzia falcataria, and Malaysiasca phaii (incl. Malaysiasca gen. nov.) on Phaius reflexipetalus. Two species are newly described from human patients in the Czech Republic, namely Microascus longicollis (from toenails of patient with suspected onychomycosis), and Chrysosporium echinulatum (from sole skin of patient). Furthermore, Alternaria quercicola is described on leaves of Quercus brantii (Iran), Stemphylium beticola on leaves of Beta vulgaris (The Netherlands), Scleroderma capever-deanum on soil (Cape Verde Islands), Scleroderma dunensis on soil, and Blastobotrys meliponae from bee honey (Brazil), Ganoderma mbrekobenum on angiosperms (Ghana), Geoglossum raitviirii and Entoloma kruticianum on soil (Russia), Priceomyces vitoshaensis on Pterostichus melas (Carabidae) (Bulgaria) is the only one for which the family is listed, Ganoderma ecuadoriense on decaying wood (Ecuador), Thyrostroma cornicola on Cornus officinalis (Korea), Cercophora vinosa on decorticated branch of Salix sp. (France), Coprinus pinetorum, Coprinus littoralis and Xerocomellus poederi on soil (Spain). Two new genera from Colombia include Helminthosporiella and Uwemyces on leaves of Elaeis oleifera. Two species are described from India, namely Russula intervenosa (ectomycorrhizal with Shorea robusta), and Crinipellis odorata (on bark of Mytragyna parviflora). Novelties from Thailand include Cyphellophora gamsii (on leaf litter), Pisolithus aureosericeus and Corynascus citrinus (on soil). Two species are newly described from Citrus in Italy, namely Dendryphiella paravinosa on Citrus sinensis, and Ramularia citricola on Citrus floridana. Morphological and culture characteristics along with ITS nrDNA barcodes are provided for all taxa. C1 [Crous, P. W.; Giraldo, A.; Guarnaccia, V.; Hernandez-Restrepo, M.; Woudenberg, J. H. C.; Groenewald, M.; Lombard, L.; Smith, M. Th.; Groenewald, J. Z.] CBS KNAW Fungal Biodivers Ctr, POB 85167, NL-3508 AD Utrecht, Netherlands. [Crous, P. W.; Giraldo, A.; Hernandez-Restrepo, M.] Univ Pretoria, Forestry & Agr Biotechnol Inst FABI, Dept Microbiol & Plant Pathol, P Bag X20, ZA-0028 Pretoria, South Africa. [Wingfield, M. J.; Boekhout, T.; Roux, J.] Univ Pretoria, Forestry & Agr Biotechnol Inst FABI, ZA-0002 Pretoria, South Africa. [Richardson, D. M.; Le Roux, J. J.] Univ Stellenbosch, Dept Bot & Zool, Ctr Invas Biol, ZA-7602 Matieland, South Africa. [Strasberg, D.] Univ La Reunion, UMR PVBMT, Peuplements Vegetaux & Bioagresseurs Milieu Trop, CS 93002, 15 Ave Rena Cassin, St Denis 97744 9, Reunion. [Edwards, J.; Pascoe, I. G.] La Trobe Univ, Dept Econ Dev Jobs Transport & Resources, Ctr AgriBiosci, AgriBio, 5 Ring Rd, Bundoora, Vic 3083, Australia. [Roets, F.] Univ Stellenbosch, Dept Conservat Ecol & Entomol, Matieland, South Africa. [Hubka, V.; Cmokova, A.] Charles Univ Prague, Fac Sci, Dept Bot, Benatska 2, Prague 12801 2, Czech Republic. [Taylor, P. W. J.] Univ Melbourne, Fac Vet & Agr Sci, Melbourne, Vic 3010, Australia. [Heykoop, M.; Moreno, G.; Carlavilla, J. R.; Manjon, J. L.; Esteve-Raventos, F.; Sanchez, A.] Univ Alcala De Henares, Dept Ciencias Vida, Area Bot, E-28805 Madrid, Spain. [Martin, M. P.; Duenas, M.; Telleria, M. T.] Real Jardin Bot CSIC, Dept Micol, Plaza Murillo 2, Madrid 28014, Spain. [Sutton, D. A.; Wiederhold, N. P.] Univ Texas Hlth Sci Ctr San Antonio, Dept Pathol, Fungus Testing Lab, 7703 Floyd Curl Dr, San Antonio, TX 78229 USA. [Barnes, C. W.] Inst Nacl Invest Agr, Estac Expt Santa Catalina, Dept Nacl Protecc Vegetal, Panamericana Sur Km 1 Via Tambillo, Quito, Provincia Pichi, Ecuador. [Gene, J.; Guarro, J.; Sandoval-Denis, M.; Cano-Lira, J. F.; Stchigel, A. M.; Valenzuela-Lopez, N.] Univ Rovira & Virgili URV, Mycol Unit, Sch Med, Sant Llorenc 21, Tarragona 43201, Spain. [Gene, J.; Guarro, J.; Sandoval-Denis, M.; Cano-Lira, J. F.; Stchigel, A. M.; Valenzuela-Lopez, N.] Univ Rovira & Virgili URV, IISPV, Sant Llorenc 21, Tarragona 43201, Spain. [Kolarik, M.] Inst Microbiol AS CR, Vvi, Lab Fungal Genet & Metab, Videnska 1083, Prague 14220 2, Czech Republic. [Popov, E. S.; Fedosova, A. G.; Morozova, O. V.] Russian Acad Sci, Komarov Bot Inst, Lab Systemat & Geog Fungi, 2 Prof Popov Str, St Petersburg 197376, Russia. [Acharya, K.; Dutta, A. K.; Paloi, S.] Univ Calcutta, Dept Bot, Mol & Appl Mycol & Plant Pathol Lab, 35 Ballygunge Circular Rd, Kolkata 700019, W Bengal, India. [Alexandrova, A. V.; Dyakov, M. Yu.] Lomonosov Moscow State Univ MSU, Fac Biol, 1,12 Leninskie Gory Str, Moscow 119234, Russia. [Alvarado, P.] ALVALAB, La Rochela 47, E-39012 Santander, Spain. [Barbosa, R. N.; Oliveira, N. T.; Silva, G. A.] Univ Fed Pernambuco, Dept Micol Prof Chaves Batista, Recife, PE, Brazil. [Baseia, I. G.; Silva, B. D. B.] Univ Fed Rio Grande do Norte, Dept Bot & Zool, Natal, RN, Brazil. [Blanchette, R. A.; Held, B. W.; Otto, E. C.] Univ Minnesota, Dept Plant Pathol, 495 Borlaug Hall,1991 Upper Buford Circle, St Paul, MN 55108 USA. [Burgess, T. I.; Hardy, G. E. St. J.] Murdoch Univ, Ctr Phytophthora Sci & Management, 90 South St, Murdoch, WA 6150, Australia. [Dimitrov, R. A.] Sofia Univ St Kliment Ohridski, 5 James Bourchier Blvd, Sofia 1164, Bulgaria. [Gamboa, P.] Univ Cent Ecuador, Fac Med Carrera Ciencias Biol & Ambientales, Av Amer, Quito, Ecuador. [Gouliamova, D. E.; Stoilova-Disheva, M. M.] Bulgarian Acad Sci, Stephan Angeloff Inst Microbiol, Acad G Bonchev 26, Sofia 1113, Bulgaria. [Grebenc, T.] Slovenian Forestry Inst, Vecna Pot 2, Ljubljana, Slovenia. [Hanse, B.] IRS, POB 32, NL-4600 AA Bergen Op Zoom, Netherlands. [Jurjevic, Z.] EMSL Analyt Inc, 200 Route 130 North, Cinnaminson, NJ 08077 USA. [Kaewgrajang, T.] Kasetsart Univ, Fac Forestry, Dept Forest Biol, 50 Ngamwongwan Rd, Bangkok 10900, Thailand. [Latha, K. P. D.; Manimohan, P.] Univ Calicut, Dept Bot, Calicut 673635, Kerala, India. [Luangsa-ard, J. J.] BIOTEC, Microbe Interact Lab, Thailand Sci Pk, Khlong Nueng 12120, Klong Luang Pat, Thailand. [Lyskova, P.] Publ Hlth Inst Usti Labem, Dept Parasitol Mycol & Mycobacteriol Prague, Med Mycol Lab, Sokolovska 60, Prague 18600 8, Czech Republic. [Mallatova, N.] Hosp Ceske Budejovice, Lab Mycol & Parasitol, B Nemcove 585-54, Ceske Budejovice 37001, Czech Republic. [Miller, A. N.] Univ Illinois, Illinois Nat Hist Survey, 1816 South Oak St, Champaign, IL 61820 USA. [Mirabolfathy, M.] Iranian Res Inst Plant Protect, Tehran, Iran. [Obodai, M.] CSIR Food Res Inst, POB M20, Accra, Ghana. [Ordonez, M. E.; Salazar, W. A.] Pontificia Univ Catolica Ecuador, Escuela Ciencias Biol, Av 12 Octubre 1076 & Roca, Quito, Ecuador. [Peterson, S. W.] ARS, USDA, Mycotoxin Prevent & Appl Microbiol Res Unit, 1815 North Univ St, Peoria, IL 61604 USA. [Phosri, C.] Nakhon Phanom Univ, Fac Sci, 214,Moo 12,Nittayo Rd, Muang Dist 48000, Nakhon Phanom, Thailand. [Sarria, G. A.] Corp Ctr Invest Palma Aceite CENIPALMA, Bogota, Colombia. [Shin, H. -D.] Korea Univ, Div Environm Sci & Ecol Engn, Seoul 02841, South Korea. [Souza-Motta, C. M.] URM Culture Collect, Recife, PE, Brazil. [Sulzbacher, M. A.] Univ Fed Santa Maria, Dept Solos, CCR, Campus Univ, BR-97105090 Santa Maria, RS, Brazil. [Toapanta, C.] Pontificia Univ Catolica Ecuador, Escuela Ciencias Biol, Av 12 Octubre 1076 & Roca, Quito, Ecuador. [Traba, J. M.] Plaza Espana 1, E-15001 La Coruna, Spain. [Valenzuela-Lopez, N.] Univ Antofagasta, Fac Hlth Sci, Med Technol Dept, Microbiol Unit, Av Univ Antofagasta S-N, Antofagasta 02800, Chile. [Watling, R.] Caledonian Mycol Enterprises, 26 Blinkbonny Ave, Edinburgh EH4 3HU, Midlothian, Scotland. RP Crous, PW (reprint author), CBS KNAW Fungal Biodivers Ctr, POB 85167, NL-3508 AD Utrecht, Netherlands. EM p.crous@cbs.knaw.nl RI Duenas, Margarita/H-4944-2015; Groenewald, Marizeth/G-4976-2012; Kolarik, Miroslav/A-1680-2008; Silva, Gladstone/E-5110-2013; Barbosa, Renan /E-5644-2017; Oliveira, Neiva /I-2180-2012; Souza-Motta, Cristina Maria/C-3503-2013; Groenewald, Johannes/F-4667-2011; Lombard, Lorenzo/B-2042-2010; Fedosova, Anna/Q-8087-2016; Woudenberg, Joyce H.C./C-9384-2013; Popov, Eugene/J-2481-2012; Martin, Maria /H-8069-2012; Richardson, David/A-1495-2008 OI Duenas, Margarita/0000-0003-0621-8003; Groenewald, Marizeth/0000-0003-0835-5925; Kolarik, Miroslav/0000-0003-4016-0335; Barbosa, Renan /0000-0003-2659-9157; DUTTA, ARUN KUMAR/0000-0001-5234-3441; Burgess, Treena/0000-0002-7962-219X; Lombard, Lorenzo/0000-0002-5942-5375; Fedosova, Anna/0000-0002-4970-3474; Woudenberg, Joyce H.C./0000-0002-7337-6864; Popov, Eugene/0000-0001-8599-3117; Martin, Maria /0000-0002-1235-4418; Richardson, David/0000-0001-9574-8297 FU Plan Nacional I+D+I projects [CGL2009-07231, CGL2012-35559]; Russian Foundation for Basic Research [15-29-02622, 15-04-04645a]; Russian Science Foundation [14-50-00029]; Kerala State Council for Science, Technology and Environment (KSCSTE) [001/FSHP/2011/CSTE]; Principal Chief Conservator of forests, Kerala State [WL10-4937/2012]; Charles University Grant Agency [GAUK 8615]; project 'BIOCEV-Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University' from the European Regional Development Fund [CZ.1.05/1.1.00/02.0109]; Bulgarian Science Fund [D002-TK-176]; EU FP6 Research and Infrastructure Synthesis grant; DST-NRF Centre of Excellence for Invasion Biology; European Union's Horizon research innovation and staff exchange programme (RISE) under the Marie Sklodowska-Curie grant [645701]; Spanish Ministerio de Economia y Competitividad grant [CGL 2011-27185]; Secretaria de Educacion Superior, Ciencia, Tecnologia e Innovacion del Ecuador (SENESCYT, Arca de Noe Initiative) FX Financial support was provided to Maria P. Martin, Margarita Duenas and M. Teresa Telleria by Plan Nacional I+D+I projects No. CGL2009-07231 and CGL2012-35559. They also acknowledge Marian Glenn (Seton Hall University, USA) for her revision of the English text. Anna G. Fedosova and Eugene S. Popov acknowledge financial support from the Russian Foundation for Basic Research (project 15-29-02622). Gabriel Moreno, Juan Ramon Carlavilla, Michel Heykoop and Jose Luis Manjon wish to express their gratitude to Miguel Martin Calvo (Sociedad Micologica de Madrid) and Manuel Castro-Marcote for sending them fungal collections; to Dr L. Monje and Mr A. Pueblas of the Department of Drawing and Scientific Photography at the University of Alcala for their help in the digital preparation of the photographs; to Dr J. Rejos, curator of the AH herbarium, for his assistance with the specimens examined in the present study. Olga V. Morozova and Eugene S. Popov are grateful to the Russian Foundation for Basic Research (project 15-04-04645a) for financial support. Alina V. Alexandrova and Maxim Yu. Dyakov acknowledge financial support from the Russian Science Foundation (project N 14-50-00029). K.P.D. Latha acknowledges support from the Kerala State Council for Science, Technology and Environment (KSCSTE) in the form of a PhD fellowship (Grant No. 001/FSHP/2011/CSTE) and is thankful to the Principal Chief Conservator of forests, Kerala State, for granting permission (No. WL10-4937/2012, dated 03-10-2013) to collect agarics from the forests of Kerala. The research of Vit Hubka, Miroslav Kolarik and Adela Cmokova was supported through a grant from the Charles University Grant Agency (GAUK 8615) and by the project 'BIOCEV-Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University' (CZ.1.05/1.1.00/02.0109) from the European Regional Development Fund. Dilnora E. Gouliamova, Margarita M. Stoilova-Disheva and Roumen A. Dimitrov acknowledge support from the Bulgarian Science Fund (D002-TK-176) and EU FP6 Research and Infrastructure Synthesis grant. They are also grateful to Dr Borislav Gueorguiev for the identification of insects. Margarita Hernandez-Restrepo and Pedro W. Crous thank Prof. Uwe Braun (Martin-Luther Univ. Halle, Germany) for his comments on the fungi described as Helminthosporiella and Uwemyces. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. Johannes J. le Roux and David M. Richardson acknowledge funding from the DST-NRF Centre of Excellence for Invasion Biology. J. Jennifer Luangsa-ard, Alejandra Giraldo and Pedro W. Crous have received funding from the European Union's Horizon 2020 research innovation and staff exchange programme (RISE) under the Marie Sklodowska-Curie grant agreement No. 645701. Francois Roets and Pedro W. Crous thank the management board of Robben Island Museum, the Western Cape Nature Conservation board and Sabelo Madlala for permission to undertake research on Robben Island. Josepa Gene acknowledges financial support from the Spanish Ministerio de Economia y Competitividad grant CGL 2011-27185. Maria E. Ordonez and colleagues acknowledge financial support from Secretaria de Educacion Superior, Ciencia, Tecnologia e Innovacion del Ecuador (SENESCYT, Arca de Noe Initiative). NR 152 TC 5 Z9 5 U1 19 U2 19 PU RIJKSHERBARIUM PI LEIDEN PA PO BOX 9514, 2300 RA LEIDEN, NETHERLANDS SN 0031-5850 J9 PERSOONIA JI Persoonia PD JUN PY 2016 VL 36 SI SI BP 316 EP 458 DI 10.3767/003158516X692185 PG 143 WC Mycology SC Mycology GA DY7CQ UT WOS:000385287400011 PM 27616795 ER PT J AU Hanks, EM Hooten, MB Knick, ST Oyler-McCance, SJ Fike, JA Cross, TB Schwartz, MK AF Hanks, Ephraim M. u Hooten, Mevin B. Knick, Steven T. Oyler-McCance, Sara J. Fike, Jennifer A. Cross, Todd B. Schwartz, Michael K. TI LATENT SPATIAL MODELS AND SAMPLING DESIGN FOR LANDSCAPE GENETICS SO ANNALS OF APPLIED STATISTICS LA English DT Article DE Landscape genetics; sage grouse; optimal sampling ID GREATER SAGE-GROUSE; CIRCUIT-THEORY; NATURAL-POPULATIONS; CONNECTIVITY; RESISTANCE; INFERENCE; FLOW; PREDICTION; DYNAMICS AB We propose a spatially-explicit approach for modeling genetic variation across space and illustrate how this approach can be used to optimize spatial prediction and sampling design for landscape genetic data. We propose a multinomial data model for categorical microsatellite allele data commonly used in landscape genetic studies, and introduce a latent spatial random effect to allow for spatial correlation between genetic observations. We illustrate how modern dimension reduction approaches to spatial statistics can allow for efficient computation in landscape genetic statistical models covering large spatial domains. We apply our approach to propose a retrospective spatial sampling design for greater sage-grouse (Centrocercus urophasianus) population genetics in the western United States. C1 [Hanks, Ephraim M. u] Penn State Univ, Dept Stat, University Pk, PA 16802 USA. [Hooten, Mevin B.] US Geol Survey, Colorado Cooperat Fish & Wildlife Res Unit, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Hooten, Mevin B.] Colorado State Univ, Dept Stat, Ft Collins, CO 80523 USA. [Knick, Steven T.] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Boise, ID 83706 USA. [Oyler-McCance, Sara J.; Fike, Jennifer A.] US Geol Survey, Ft Collins Sci Ctr, 2150 Ctr Ave Bldg C, Ft Collins, CO 80526 USA. [Cross, Todd B.; Schwartz, Michael K.] US Geol Survey, Rocky Mt Res Stn, Henderson, NV USA. [Cross, Todd B.] Univ Montana, Missoula, MT 59812 USA. [Cross, Todd B.; Schwartz, Michael K.] Natl Genom Ctr Wildlife & Fish Conservat, USFS Rocky Mt Res Stn, 800 E Beckwith Ave, Missoula, MT 59801 USA. RP Hanks, EM (reprint author), Penn State Univ, Dept Stat, University Pk, PA 16802 USA. EM hanks@psu.edu FU U.S. Geological Survey [RWO 98] FX Supported by the U.S. Geological Survey RWO 98. NR 48 TC 1 Z9 1 U1 4 U2 4 PU INST MATHEMATICAL STATISTICS PI CLEVELAND PA 3163 SOMERSET DR, CLEVELAND, OH 44122 USA SN 1932-6157 J9 ANN APPL STAT JI Ann. Appl. Stat. PD JUN PY 2016 VL 10 IS 2 BP 1041 EP 1062 DI 10.1214/16-AOAS929 PG 22 WC Statistics & Probability SC Mathematics GA DY3XA UT WOS:000385029700021 ER PT J AU Di Gioia, F Ozores-Hampton, M Hong, J Kokalis-Burelle, N Albano, J Zhao, X Black, Z Gao, ZF Wilson, C Thomas, J Moore, K Swisher, M Guo, HC Rosskopf, EN AF Di Gioia, Francesco Ozores-Hampton, Monica Hong, Jason Kokalis-Burelle, Nancy Albano, Joseph Zhao, Xin Black, Zack Gao, Zhifeng Wilson, Chris Thomas, John Moore, Kelly Swisher, Marilyn Guo, Haichao Rosskopf, Erin N. TI The Effects of Anaerobic Soil Disinfestation on Weed and Nematode Control, Fruit Yield, and Quality of Florida Fresh-market Tomato SO HORTSCIENCE LA English DT Article DE Solanum lycopersicum; biological soil disinfestation; chemical soil fumigation; molasses; methyl bromide alternatives ID SOILBORNE PLANT-PATHOGENS; ORGANIC AMENDMENTS; NITROGEN RATES; USE EFFICIENCY; SANDY SOIL; MECHANISMS; BIOMASS; ACCUMULATION; SOLARIZATION; SUPPRESSION AB Anaerobic soil disinfestation (ASD) is considered a promising sustainable alternative to chemical soil fumigation (CSF), and has been shown to be effective against soilborne diseases, plant-parasitic nematodes, and weeds in several crop production systems. Nevertheless, limited information is available on the effects of ASD on crop yield and quality. Therefore, a field study was conducted on fresh-market tomato (Solanum lycopersicum L.) in two different locations in Florida (Immokalee and Citra), to evaluate and compare the ASD and CSF performances on weed and nematodes control, and on fruit yield and quality. In Immokalee, Pic-Clor 60 (1,3-dichloropropene + chloropicrin) was used as the CSF, whereas in Citra, the CSF was Paldin (TM) [dimethyl disulfide (DMDS) + chloropicrin]. Anaerobic soil disinfestation treatments were applied using a mix of composted poultry litter (CPL) at the rate of 22 Mg.ha(-1), and two rates of molasses [13.9 (ASD1) and 27.7 m(3).ha(-1) (ASD2)] as a carbon (C) source. In both locations, soil subjected to ASD reached highly anaerobic conditions, and cumulative soil anaerobiosis was 167% and 116% higher in ASD2 plots than in ASD1 plots, in Immokalee and Citra, respectively. In Immokalee, the CSF provided the most significant weed control, but ASD treatments also suppressed weeds enough to prevent an impact on yield. In Citra, all treatments, including the CSF, provided poor weed control relative to the Immokalee site. In both locations, the application of ASD provided a level of root-knot nematode (Meloidogyne sp.) control equivalent to, or more effective than the CSF. In Immokalee, ASD2 and ASD1 plots provided 26.7% and 19.7% higher total marketable yield as compared with CSF plots, respectively. However, in Citra, total marketable yield was unaffected by soil treatments. Tomato fruit quality parameters were not influenced by soil treatments, except for fruit firmness in Immokalee, which was significantly higher in fruits from ASD treatments than in those from CSF soil. Fruit mineral content was similar or higher in ASD plots as compared with CSF. In fresh-market tomato, ASD applied using a mixture of CPL and molasses may be a sustainable alternative to CSF for maintaining or even improving marketable yield and fruit quality. C1 [Di Gioia, Francesco; Ozores-Hampton, Monica] Univ Florida, Inst Food & Agr Sci, SW Florida Res & Educ Ctr, Immokalee, FL 34142 USA. [Hong, Jason; Kokalis-Burelle, Nancy; Albano, Joseph; Rosskopf, Erin N.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Zhao, Xin; Black, Zack; Guo, Haichao] Univ Florida, Dept Hort Sci, Gainesville, FL 32611 USA. [Gao, Zhifeng] Univ Florida, Food & Resource Econ, Gainesville, FL 32611 USA. [Moore, Kelly] Univ Florida, Ctr Sustainable & Organ Food Syst, Gainesville, FL 32611 USA. [Swisher, Marilyn] Univ Florida, Sustainable Agr Family Youth & Community Sci, Gainesville, FL 32611 USA. RP Di Gioia, F (reprint author), Univ Florida, Inst Food & Agr Sci, SW Florida Res & Educ Ctr, Immokalee, FL 34142 USA. EM fdigioia@ufl.edu FU USDA, ARS, Areawide Project on Anaerobic Soil Disinfestation FX The research presented here was funded in part by the USDA, ARS, Areawide Project on Anaerobic Soil Disinfestation. NR 45 TC 0 Z9 0 U1 11 U2 11 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD JUN PY 2016 VL 51 IS 6 BP 703 EP 711 PG 9 WC Horticulture SC Agriculture GA DY3HQ UT WOS:000384982000008 ER PT J AU Ledbetter, CA AF Ledbetter, Craig A. TI 'Valley Pearl' Table Grape SO HORTSCIENCE LA English DT Editorial Material DE cultivar; fruit breeding; Vitis vinifera ID VITIS-VINIFERA; SEEDLESS C1 [Ledbetter, Craig A.] Agr Res Serv, USDA, San Joaquin Valley Agr Sci Ctr, 9611 S Riverbend Ave, Parlier, CA 93648 USA. RP Ledbetter, CA (reprint author), Agr Res Serv, USDA, San Joaquin Valley Agr Sci Ctr, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM craig.ledbetter@ars.usda.gov NR 5 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD JUN PY 2016 VL 51 IS 6 BP 772 EP 774 PG 3 WC Horticulture SC Agriculture GA DY3HQ UT WOS:000384982000020 ER PT J AU De Oliveira, ML Thomson, JG Stover, E AF De Oliveira, Maria Luiza Thomson, James G. Stover, Ed TI High-efficiency Propagation of Mature 'Washington Navel' Orange and Juvenile 'Carrizo' Citrange Using Axillary Shoot Proliferation SO HORTTECHNOLOGY LA English DT Article DE basal salt formulation; Citrus sinensis; C. sinensis x Poncirus trifoliata; Driver and Kuniyuki walnut medium; nodal explants; plant growth regulators; rooting in vitro; spermidine ID IN-VITRO PROPAGATION; GROWTH; MICROPROPAGATION; POLYAMINES; LIME AB In vitro axillary shoot proliferation can be used to increase availability of citrus (Citrus) types in high demand, while limiting somaclonal variation. However, established protocols could be improved to increase efficiency. Therefore, this study investigated some factors [plant growth regulators (PGRs), basal media, and successive subculturing] which affect the in vitro axillary shoot proliferation of mature 'Washington Navel' orange (Citrus sinensis) and juvenile 'Carrizo' citrange (C. sinensis X Poncirus trifoliata). In 'Washington Navel' orange, maximum axillary shoot induction (66.9% explants producing axillary shoots with a mean of 2.45 shoots per explant) was obtained in Driver and Kuniyuki walnut (DKW) medium supplemented with 0.1 mg.L-1 6-benzylaminopurine (BA), 0.05 mg.L-1 naphthalene acetic acid (NAA) along with 1 mg.L-1 6-furfurylaminopurine [kinetin (kin)], whereas in 'Carrizo' citrange, axillary shoot production was greatest (82.6% and 87.5% of explants producing axillary shoots with a mean of 4.3 and 4.1 shoots per explant) at 1.0 or 2.0 mg.L-1 BA in DKW medium. The initial nodal propagules (with basal tissue remaining from removed shoots) were repeatedly subcultured for six times every 4 weeks onto DKW medium with the same levels of PGRs used for initial culturing. Woody plant medium (WPM), Murashige and Skoog medium (MS), and DKW were also compared for rooting at quarter to full strength for salt components, all amended with 2.0 mg.L-1 indolebutyric acid (IBA) and 0.5 mg.L-1 NAA. MS at full strength provided the highest rooting in 'Carrizo' citrange (93%) and longest root length (58 mm), whereas half-strength MS provided the highest rooting in 'Washington Navel' orange (60% to 61%) and the longest roots (26 mm). Addition of 1 mu M spermidine to the rooting medium enhanced root length only for 'Washington Navel' orange on full-strength MS, but accelerated rooting for both cultivars on all media. The plantlets were successfully transferred to greenhouse conditions, exhibiting normal development, with high uniformity, and no evidence of somaclonal variation. C1 [De Oliveira, Maria Luiza; Stover, Ed] ARS, Subtrop Insects & Hort Res Unit, USDA, Ft Pierce, FL 34945 USA. [Thomson, James G.] ARS, Crop Improvement & Utilizat, USDA, Albany, CA 94710 USA. RP Stover, E (reprint author), ARS, Subtrop Insects & Hort Res Unit, USDA, Ft Pierce, FL 34945 USA. EM ed.stover@ars.usda.gov FU California Citrus Research Board [5200-140A]; U.S. Department of Agriculture, Agricultural Research Service, CRIS projects [5325-21000-018, 5325-21000-020, 6618-21000-014-00]; USDA-National Institute of Food and Agriculture [2010-33522-21773] FX This research was financially supported by the California Citrus Research Board 5200-140A. This research was also supported by U.S. Department of Agriculture, Agricultural Research Service, CRIS projects 5325-21000-018, 5325-21000-020, and 6618-21000-014-00, and by the Biotechnology Risk Assessment Program competitive grant number 2010-33522-21773 from the USDA-National Institute of Food and Agriculture. NR 30 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD JUN PY 2016 VL 26 IS 3 BP 278 EP 286 PG 9 WC Horticulture SC Agriculture GA DY3IE UT WOS:000384983800005 ER PT J AU Zhang, HB Richardson, PA Belayneh, BE Ristvey, A Lea-Cox, J Copes, WE Moorman, GW Hong, CX AF Zhang, Haibo Richardson, Patricia A. Belayneh, Bruk E. Ristvey, Andrew Lea-Cox, John Copes, Warren E. Moorman, Gary W. Hong, Chuanxue TI RECYCLING IRRIGATION RESERVOIR STRATIFICATION AND IMPLICATIONS FOR CROP HEALTH AND PRODUCTION SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE recycling irrigation reservoir; thermal stratification; recycled water quality; dissolved oxygen; pH; water management ID THERMAL STRATIFICATION; DISSOLVED-OXYGEN; WATER WITHDRAWAL; TURBID WATERBODY; URBAN PONDS; SP-NOV; SHALLOW; LAKE; TEMPERATURE; PHYTOPLANKTON AB Recycling irrigation reservoirs (RIRs) are an emerging aquatic ecosystem and water resource of global significance. This study investigated the vertical distribution of water temperature, dissolved oxygen (DO), and pH in eight RIRs at two nurseries each in Virginia and Maryland from 2011 to 2014. Monomictic thermal stratification was observed from April to October in all RIRs, despite their shallow depths (0.75-3.89 m). The strongest stratification had a top-bottom temperature difference of 21.53 degrees C. The top-bottom temperature difference was positively correlated with water column depth, air temperature, and daily light integral (p < 0.05). Wind speed did not impact the thermal stratification, likely due to their relatively small surface areas. Thermal stratification affected the vertical distribution of DO and pH. The top-bottom differences in DO and pH were greater during stratification periods than nonstratification periods. Water pH in all RIRs was higher at the top than at the bottom with the greatest difference of 4.16 units. Discovery and characterization of thermal stratification in RIRs helps understand water quality dynamics in this novel ecosystem and promote safe and productive water reuse for irrigation. Specifically, water withdrawal depths should be adjusted according to variations in temperature, DO, and pH during the stratification and nonstratification periods to mitigate pathogen risk and improve water treatment efficacy and crop production. C1 [Zhang, Haibo; Richardson, Patricia A.; Hong, Chuanxue] Virginia Polytech Inst & State Univ, Hampton Rd Agr Res & Extens Ctr, 1444 Diamond Springs Rd, Virginia Beach, VA 23455 USA. [Belayneh, Bruk E.; Lea-Cox, John] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA. [Ristvey, Andrew] Univ Maryland Extens, Wye Res & Educ Ctr, Queenstown, MD 21658 USA. [Copes, Warren E.] ARS, USDA, Poplarville, MS 39470 USA. [Moorman, Gary W.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. RP Zhang, HB (reprint author), Virginia Polytech Inst & State Univ, Hampton Rd Agr Res & Extens Ctr, 1444 Diamond Springs Rd, Virginia Beach, VA 23455 USA. EM haiboz85@vt.edu FU Virginia Agricultural Experiment Station; USDA National Institute of Food and Agriculture Specialty Crop Research Initiative [2010-51181-21140]; Hatch Program FX The data for this article can be accessed from http://hdl.handle.net/10919/50561 at VTechWorks at Virginia Polytechnic Institute and State University. This research was supported in part by Virginia Agricultural Experiment Station, the USDA National Institute of Food and Agriculture Specialty Crop Research Initiative (Agreement number 2010-51181-21140), and Hatch Program. We thank collaborating growers for providing access to the study reservoirs and on-site assistances. We thank Frank Dugan and Xiao Yang for their critical review of the manuscript. NR 54 TC 1 Z9 1 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD JUN PY 2016 VL 52 IS 3 BP 620 EP 631 DI 10.1111/1752-1688.12411 PG 12 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA DY3PW UT WOS:000385007100005 ER PT J AU Salo, JA Theobald, DM Brown, TC AF Salo, Jessica A. Theobald, David M. Brown, Thomas C. TI EVALUATION OF METHODS FOR DELINEATING RIPARIAN ZONES IN A SEMI-ARID MONTANE WATERSHED SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE riparian zone delineation; riparian ecology; geospatial analysis; spatial modeling; uniform and variable-width buffers; valley bottom topography; upstream drainage area ID UNITED-STATES; ECOSYSTEM SERVICES; STREAMS; AREAS; CONSERVATION; RESTORATION; PERSPECTIVE; ATTRIBUTES; MANAGEMENT; RESOLUTION AB Riparian zones in semi-arid, mountainous regions provide a disproportionate amount of the available wildlife habitat and ecosystem services. Despite their importance, there is little guidance on the best way to map riparian zones for broad spatial extents (e.g., large watersheds) when detailed maps from field data or high-resolution imagery and terrain data are not available. Using well-established accuracy metrics (e.g., kappa, precision, computational complexity), we evaluated eight methods commonly used to map riparian zones. Focusing on a semi-arid, mountainous watershed, we found that the most accurate and robust method for mapping riparian zones combines data on upstream drainage area and valley topography. That method performed best regardless of stream order, and was most effective when implemented with fine resolution topographic and stream line data. Other commonly used methods to model riparian zones, such as those based on fixed-width buffers, yielded inaccurate results. We recommend that until very-high resolution (< 1 m) elevation data are available at broad extents, models of riparian zones for semi-arid mountainous regions should incorporate drainage area, valley topography, and quantify uncertainty. C1 [Salo, Jessica A.] Univ Northern Colorado, Geog & GIS Dept, Box 115, Greeley, CO 80639 USA. [Theobald, David M.] Conservat Sci Partners, Ft Collins, CO 80524 USA. [Brown, Thomas C.] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Salo, JA (reprint author), Univ Northern Colorado, Geog & GIS Dept, Box 115, Greeley, CO 80639 USA. EM jessica.salo@unco.edu FU Rocky Mountain Research Station, U.S. Forest Service FX This research was supported in part by funds from the Rocky Mountain Research Station, U.S. Forest Service. The authors thank Josh Goldstein, Dylan Harrison-Atlas, Stephanie Kampf, Sam Litschert, and David Merritt for their thoughtful suggestions. NR 55 TC 0 Z9 0 U1 5 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD JUN PY 2016 VL 52 IS 3 BP 632 EP 647 DI 10.1111/1752-1688.12414 PG 16 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA DY3PW UT WOS:000385007100006 ER PT J AU Yarnell, S Peek, R Epke, G Lind, A AF Yarnell, Sarah Peek, Ryan Epke, Gerhard Lind, Amy TI MANAGEMENT OF THE SPRING SNOWMELT RECESSION IN REGULATED SYSTEMS SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE water resources management; aquatic ecology; rivers/streams; streamflow; fish ID FLOW REGIME; HABITAT SUITABILITY; RIVER; CALIFORNIA; DAMS; HYDROGRAPHS; ECOSYSTEMS; STREAM AB In an effort to restore predictable ecologically relevant spring snowmelt recession flow patterns in rivers regulated by dams, this study defined a methodology by which spring flow regimes can be modeled in regulated systems from the quantifiable characteristics of spring snowmelt recessions in unregulated rivers. An analysis of eight unregulated rivers across the Sierra Nevada mountain range in California found that unregulated systems behaved similarly with respect to seasonal spring patterns and recession limb curvature, and thus prescribed flows could be designed in a manner that mimics those predictable characteristics. Using the methodology to quantify spring recession flows in terms of a daily percent decrease in flow, a series of flow recession scenarios were created for application in an existing hydrodynamic model for the regulated Rubicon River. The modeling results showed that flow recessions with slow ramping rates similar to those observed in unregulated rivers (less than 10% per day) were likely to be protective of native aquatic species, such as the Foothill yellow-legged frog, while flows that receded at greater rates would likely result in desiccation of egg masses and potential stranding of tadpoles and fry. Furthermore, recession rates of less than 10% per day provided the most spatially diverse hydraulic habitat in the modeled domain for an appropriate duration in spring to support all native species guilds and maximize aquatic biodiversity. C1 [Yarnell, Sarah; Peek, Ryan] Univ Calif Davis, Ctr Watershed Sci, One Shields Ave, Davis, CA 95616 USA. [Epke, Gerhard] Town San Anselmo, Publ Works Dept, Marin Cty, CA 94960 USA. [Lind, Amy] US Forest Serv, Tahoe Natl Forest, Nevada City, CA 95959 USA. RP Yarnell, S (reprint author), Univ Calif Davis, Ctr Watershed Sci, One Shields Ave, Davis, CA 95616 USA. EM smyarnell@ucdavis.edu FU Public Interest Energy Research Program of the California Energy Commission [500-2014-030]; John Muir Institute of the Environment at the University of California, Davis FX We thank Placer County Water Agency for the generous use of their data and hydrodynamic model for this study. This research was supported and funded by the Public Interest Energy Research Program of the California Energy Commission under contract number 500-2014-030 and by the John Muir Institute of the Environment at the University of California, Davis. NR 29 TC 0 Z9 0 U1 8 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD JUN PY 2016 VL 52 IS 3 BP 723 EP 736 DI 10.1111/1752-1688.12424 PG 14 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA DY3PW UT WOS:000385007100011 ER PT J AU Steel, EA Sowder, C Peterson, EE AF Steel, E. Ashley Sowder, Colin Peterson, Erin E. TI SPATIAL AND TEMPORAL VARIATION OF WATER TEMPERATURE REGIMES ON THE SNOQUALMIE RIVER NETWORK SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE river network; time series analysis; spatial modeling; thermal regime; wavelet decomposition; metric ID STREAM TEMPERATURE; PACIFIC-NORTHWEST; WAVELET ANALYSIS; THERMAL REGIMES; CLIMATE-CHANGE; VARIABILITY; RESOURCE; WILDFIRE; ECOLOGY; HABITAT AB Although mean temperatures change annually and are highly correlated with elevation, the entire thermal regime on the Snoqualmie River, Washington, USA does not simply shift with elevation or season. Particular facets of the thermal regime have unique spatial patterns on the river network and at particular times of the year. We used a spatially and temporally dense temperature dataset to generate 13 temperature metrics representing popular summary measures (e.g., minimum, mean, or maximum temperature) and wavelet variances over each of seven time windows. Spatial stream-network models which account for within-network dependence were fit using three commonly used predictors of riverine thermal regime (elevation, mean annual discharge, and percent commercial area) to each temperature metric in each time window. Predictors were strongly related (r(2) > 0.6) to common summaries of the thermal regime but were less effective at describing other facets of the thermal regime. Relationships shifted with season and across facets. Summer mean temperatures decreased strongly with increasing elevation but this relationship was weaker for winter mean temperatures and winter minimum temperatures; it was reversed for mean daily range and there was no relationship between elevation and wavelet variances. We provide examples of how enriched information about the spatial and temporal complexities of natural thermal regimes can improve management and monitoring of aquatic resources. C1 [Steel, E. Ashley] US Forest Serv, Pacific Northwest Res Stn, USDA, 400 North 34th St,Suite 201, Seattle, WA 98103 USA. [Sowder, Colin] Univ Washington, Dept Stat, Seattle, WA 98195 USA. [Peterson, Erin E.] CSIRO Digital Prod Flagship, Dutton Pk, Qld, Australia. [Peterson, Erin E.] Queensland Univ Technol, ARC Ctr Excellence Math & Stat, Brisbane, Qld 4000, Australia. [Peterson, Erin E.] Queensland Univ Technol, Inst Future Environm, Brisbane, Qld 4000, Australia. RP Steel, EA (reprint author), US Forest Serv, Pacific Northwest Res Stn, USDA, 400 North 34th St,Suite 201, Seattle, WA 98103 USA. EM asteel@fs.fed.us OI Peterson, Erin/0000-0003-2992-0372 FU USDA Forest Service, Pacific Northwest Research Station; National Center for Ecological Analysis and Synthesis, a Center - National Science Foundation [EF-0553768]; University of California, Santa Barbara; State of California FX This project was supported by the USDA Forest Service, Pacific Northwest Research Station. This research was conducted as part of a Spatial Statistics for Streams working group supported by the National Center for Ecological Analysis and Synthesis (Project 12637), a Center funded by the National Science Foundation (Grant #EF-0553768), the University of California, Santa Barbara, and the State of California. We thank Aimee Fullerton of NOAA's Northwest Fisheries Science Center for helping to design and implement our monitoring system. We are also grateful for the field assistance of Sara Anzalone, Conamara Burke, Dongzi Liu, Harry Podschwit, and Spus Wilder. NR 59 TC 0 Z9 0 U1 6 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD JUN PY 2016 VL 52 IS 3 BP 769 EP 787 DI 10.1111/1752-1688.12423 PG 19 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA DY3PW UT WOS:000385007100014 ER PT J AU Yen, H White, MJ Ascough, JC Smith, DR Arnold, JG AF Yen, Haw White, Michael J. Ascough, James C., II Smith, Douglas R. Arnold, Jeffrey G. TI AUGMENTING WATERSHED MODEL CALIBRATION WITH INCORPORATION OF ANCILLARY DATA SOURCES AND QUALITATIVE SOFT DATA SOURCES SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE constrained calibration; soft and hard data; IPEAT; SWAT ID COON CREEK BASIN; SIMULATIONS; UNCERTAINTY; VALIDATION; IMPACT; USA AB Watershed simulation models such as the Soil & Water Assessment Tool (SWAT) can be calibrated using "hard data" such as temporal streamflow observations; however, users may find upon examination of model outputs, that the calibrated models may not reflect actual watershed behavior. Thus, it is often advantageous to use "soft data" (i.e., qualitative knowledge such as expected denitrification rates that observed time series do not typically exist) to ensure that the calibrated model is representative of the real world. The primary objective of this study is to evaluate the efficacy of coupling SWAT-Check (a post-evaluation framework for SWAT outputs) and IPEAT-SD (Integrated Parameter Estimation and Uncertainty Analysis Tool-Soft & hard Data evaluation) to constrain the bounds of soft data during SWAT auto-calibration. IPEAT-SD integrates 59 soft data variables to ensure SWAT does not violate physical processes known to occur in watersheds. IPEAT-SD was evaluated for two case studies where soft data such as denitrification rate, nitrate attributed from subsurface flow to total discharge ratio, and total sediment loading were used to conduct model calibration. Results indicated that SWAT model outputs may not satisfy reasonable soft data responses without providing predefined bounds. IPEAT-SD provides an efficient and rigorous framework for users to conduct future studies while considering both soft data and traditional hard information measures in watershed modeling. C1 [Yen, Haw] Texas A&M Agrilife Res, Blackland Res & Extens Ctr, 720 East Blackland Rd, Temple, TX 76502 USA. [White, Michael J.; Smith, Douglas R.; Arnold, Jeffrey G.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Ascough, James C., II] USDA ARS, Agr Syst Res Unit, Ft Collins, CO 80526 USA. RP Yen, H (reprint author), Texas A&M Agrilife Res, Blackland Res & Extens Ctr, 720 East Blackland Rd, Temple, TX 76502 USA. EM hyen@brc.tamus.edu FU United States Department of Agriculture-Natural Resources Conservation Service (USDA-NRCS) Conservation Effects Assessment Project (CEAP) FX This project was funded by grants from United States Department of Agriculture-Natural Resources Conservation Service (USDA-NRCS) Conservation Effects Assessment Project (CEAP)-Wildlife and Cropland components. USDA is an equal opportunity employer and provider! NR 30 TC 1 Z9 1 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD JUN PY 2016 VL 52 IS 3 BP 788 EP 798 DI 10.1111/1752-1688.12428 PG 11 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA DY3PW UT WOS:000385007100015 ER PT J AU Thoen, CO Kaplan, B Thoen, TC Gilsdorf, MJ Shere, JA AF Thoen, Charles O. Kaplan, Bruce Thoen, Tyler C. Gilsdorf, Michael J. Shere, Jack A. TI ZOONOTIC TUBERCULOSIS. A COMPREHENSIVE ONE HEALTH APPROACH SO MEDICINA-BUENOS AIRES LA English DT Article DE Mycobacterium tuberculosis; tuberculosis; M. bovis; bovine tuberculosis; One Health Approach ID MYCOBACTERIUM-BOVIS; UNITED-STATES; TRANSMISSION AB The objective of this report is to provide information on Mycobacterium tuberculosis complex infections in animals and in humans. Included is information on the susceptibility of different species as well as information on etiology, epidemiology, pathogenesis, diagnosis, prevention and control of this disease. The term One Health has been adopted to describe the unified human medical and veterinary interdisciplinary/multidisciplinary collaborative approach to zoonoses and will be critical for future endeavors in the control of the global TB epidemic. This unified paradigm is ideally suited for control of bovine TB and many other international public health and clinical health issues. Sharing resources and increasing interaction between public health and veterinary medical scientists can raise awareness of 'shared risk' of bovine TB between humans and animals and, in resource-limited situations, can maximize use of existing infrastructure and reduce unnecessary duplication of effort in disease control programs. C1 [Thoen, Charles O.] Iowa State Univ, Coll Vet Med, Dept Vet Microbiol & Prevent Med, Ames, IA USA. [Kaplan, Bruce] One Hlth Initiat, Sarasota, FL USA. [Thoen, Tyler C.] McFarland Clin, Board Certificat Internal Med, Ames, IA USA. [Gilsdorf, Michael J.] USDA, Natl Ctr Anim Hlth Programs, Washington, DC 20250 USA. [Shere, Jack A.] USDA, Vet Serv, Anim & Plant Hlth Inspect Serv, Washington, DC 20250 USA. RP Thoen, CO (reprint author), Iowa State Univ, Coll Vet Med, Vet Microbiol & Prevent Med, Ames, IA 50011 USA. EM cthoen@iastate.edu NR 24 TC 0 Z9 0 U1 4 U2 4 PU MEDICINA (BUENOS AIRES) PI BUENOS AIRES PA DONATO ALVAREZ 3150, 1427 BUENOS AIRES, ARGENTINA SN 0025-7680 EI 1669-9106 J9 MEDICINA-BUENOS AIRE JI Med.-Buenos Aires PD JUN PY 2016 VL 76 IS 3 BP 159 EP 165 PG 7 WC Medicine, General & Internal SC General & Internal Medicine GA DY3WW UT WOS:000385029200006 PM 27295705 ER PT J AU McPherson, EG van Doorn, N de Goede, J AF McPherson, E. Gregory van Doorn, Natalie de Goede, John TI Structure, function and value of street trees in California, USA SO URBAN FORESTRY & URBAN GREENING LA English DT Article DE Community forest; Municipal forest; Tree benefits; Urban ecosystem services ID DIVERSITY; FORESTS AB This study compiled recent inventory data from 929,823 street trees in 50 cities to determine trends in tree number and density, identify priority investments and create baseline data against which the efficacy of future practices can be evaluated. The number of street trees increased from 5.9 million in 1988 to 9.1 million in 2014, about one for every four residents. Street tree density declined from 65.6 to 46.6 trees per km, nearly a 30% drop. City streets are at 36.3% of full stocking. State-wide, only London plane-tree (Platanus x hispanica) comprises over 10% of the total, suggesting good state-wide species diversity. However, at the city scale, 39 communities were overly reliant on a single species. The state's street trees remove 567,748 t CO2 (92,253 t se) annually, equivalent to taking 120,000 cars off the road. Their asset value is $2.49 billion ($75.1 million se). The annual value (USD) of all ecosystem services is $1.0 billion ($58.3 million se), or $110.63 per tree ($29.17 per capita). Given an average annual per tree management cost of $19.00, $5.82 in benefit is returned for every $1 spent. Management implications could include establishing an aggressive program to plant the 16 million vacant sites and replace removed trees, while restricting planting of overabundant species. Given the tree population's youth there is likely need to invest in pruning young trees for structure and form, which can reduce subsequent costs for treating defects in mature trees. Published by Elsevier GmbH. C1 [McPherson, E. Gregory] US Forest Serv, USDA, Pacific Southwest Res Stn, 1731 Res Pk Dr, Davis, CA 95618 USA. [van Doorn, Natalie] US Forest Serv, USDA, Pacific Southwest Res Stn, 800 Buchanan St, Albany, CA 94710 USA. [de Goede, John] Univ Calif Davis, Environm Informat Ctr, Davis, CA 95616 USA. RP McPherson, EG (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, 1731 Res Pk Dr, Davis, CA 95618 USA. EM gmcpherson@fs.fed.us; nvandoorn@fs.fed.us; jmdegoede@ucdavis.edu NR 56 TC 2 Z9 2 U1 10 U2 10 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 1618-8667 J9 URBAN FOR URBAN GREE JI Urban For. Urban Green. PD JUN 1 PY 2016 VL 17 BP 104 EP 115 DI 10.1016/j.ufug.2016.03.013 PG 12 WC Plant Sciences; Environmental Studies; Forestry; Urban Studies SC Plant Sciences; Environmental Sciences & Ecology; Forestry; Urban Studies GA DY2HL UT WOS:000384913600012 ER PT J AU Alonzo, M McFadden, JP Nowak, DJ Roberts, DA AF Alonzo, Michael McFadden, Joseph P. Nowak, David J. Roberts, Dar A. TI Mapping urban forest structure and function using hyperspectral imagery and lidar data SO URBAN FORESTRY & URBAN GREENING LA English DT Article ID LEAF-AREA INDEX; AIRBORNE LIDAR; MEDITERRANEAN FOREST; CARBON STORAGE; UNITED-STATES; TREE; VEGETATION; BIOMASS; AVIRIS; SEATTLE AB Cities measure the structure and function of their urban forest resource to optimize forest management and the provision of ecosystem services. Measurements made using plot sampling methods yield useful results including citywide or land-use level estimates of species counts, leaf area, biomass, and air pollution reduction. However, these quantities are statistical estimates made over large areas and thus are not spatially explicit. Maps of forest structure and function at the individual tree crown scale can enhance management decision-making and improve understanding of the spatial distribution of ecosystem services relative to humans and infrastructure. In this research we used hyperspectral imagery and waveform lidar data to directly map urban forest species, leaf area index (LAI), and carbon storage in downtown Santa Barbara, California. We compared these results to estimates produced using field-plot sampling and the i-Tree Eco model. Remote sensing methods generally reduced uncertainty in species-level canopy cover estimates compared to field-plot methods. This was due to high classification accuracy for species with large canopies (e.g., Platanus racemosa with similar to 90% average accuracy, Pinus pinea at similar to 93%, Quercus agrifolia at similar to 83%) and high standard error of the plot-based estimates due to the uneven distribution of canopy throughout the city. Average LAI in canopy, based on lidar measurements was 4.47 while field measurements and allometry resulted in an LAI of 5.57. Citywide carbon storage, based on lidar measurements and allometry was estimated at 50,991 metric tons (t) and 55,900 t from plot-sampling. As others have noted, carbon density varied substantially by development intensity based largely on differences in fractional cover but less so when only evaluating canopy biomass. Using separate biomass equations for each leaf type (broadleaf, needleleaf, palm) resulted in a more accurate carbon map but a less accurate citywide estimate. (C) 2016 Elsevier GmbH. All rights reserved. C1 [Alonzo, Michael] NASA, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. [McFadden, Joseph P.; Roberts, Dar A.] Univ Calif Santa Barbara, Dept Geog, Santa Barbara, CA 93106 USA. [Nowak, David J.] SUNY Coll Environm Sci & Forestry, US Forest Serv, USDA, Northern Res Stn,Moon Lib 5, Syracuse, NY 13210 USA. RP Alonzo, M (reprint author), NASA, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. EM michael.g.alonzo@nasa.gov FU EPA-STAR Fellowship [FP-91768801-0]; National Science Foundation [BCS-0948914]; Garden Club of America Fellowship in Urban Forestry FX This research was funded by the first author's EPA-STAR Fellowship (FP-91768801-0), the National Science Foundation (BCS-0948914), and the Garden Club of America Fellowship in Urban Forestry. Special thanks to Robert Hoehn, Alexis Ellis, and Satoshi Hirabayashi for their work customizing, running, and communicating i-Tree Eco sub-models. NR 58 TC 3 Z9 3 U1 14 U2 15 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 1618-8667 J9 URBAN FOR URBAN GREE JI Urban For. Urban Green. PD JUN 1 PY 2016 VL 17 BP 135 EP 147 DI 10.1016/j.ufug.2016.04.003 PG 13 WC Plant Sciences; Environmental Studies; Forestry; Urban Studies SC Plant Sciences; Environmental Sciences & Ecology; Forestry; Urban Studies GA DY2HL UT WOS:000384913600015 ER PT J AU Selmi, W Weber, C Riviere, E Blond, N Mehdi, L Nowak, D AF Selmi, Wissal Weber, Christiane Riviere, Emmanuel Blond, Nadege Mehdi, Lotfi Nowak, David TI Air pollution removal by trees in public green spaces in Strasbourg city, France SO URBAN FORESTRY & URBAN GREENING LA English DT Article DE Air pollution; Environmental assessment; France; i-Tree Eco; Urban green spaces; Urban trees ID DRY DEPOSITION; ECOSYSTEM SERVICES; ATMOSPHERIC DEPOSITION; URBAN FORESTS; UNITED-STATES; OZONE; CANOPY; HEALTH; POLLUTANTS; VEGETATION AB This study integrates i-Tree Eco model in order to estimate air pollution removal by urban trees in Strasbourg city, France. Applied for the first time in a French city, the model shows that public trees, i.e., trees managed by the city, removed about 88 t of pollutants during one year period (from July 2012 to June 2013): about 1 ton for CO; 14 tons for NO2; 56 tons for O-3; 12 tons for PM10coarse (particles with diameter ranging from 2.5 to 10 mu m); 5 tons for PM2.5 and 1 ton for SO2. Air pollution removal varied mainly with the tree cover and the level of air pollutants concentrations. Comparison between simulated pollution removal rates and local emissions shows that public trees of Strasbourg reduce about 7% of the emitted PM10coarse in the city's atmosphere; however, effect on other air pollutants is small. Thus, our study reveals that urban trees are a significant element to reduce air pollution but are not the only solution to this problem. It's then recommended to associate planting and managing urban forest resources to other strategies that take into account the urban environment characteristics: built structures, street design, location of local sources; etc. (C) 2016 Elsevier GmbH. All rights reserved. C1 [Selmi, Wissal; Weber, Christiane; Blond, Nadege; Mehdi, Lotfi] Univ Strasbourg, CNRS, Lab Image, Environm LIVE UMR 7362, 3 Rue Argonne, F-67000 Strasbourg, France. [Riviere, Emmanuel] Assoc Surveillance & Etud Pollut Atmospher Alsace, 5 Rue Madrid, F-67300 Strasbourg, France. [Nowak, David] SUNY Coll Environm Sci & Forestry, US Forest Serv, USDA, Northern Res Stn,Moon Lib 5, Syracuse, NY 13210 USA. RP Selmi, W (reprint author), Univ Strasbourg, CNRS, Lab Image, Environm LIVE UMR 7362, 3 Rue Argonne, F-67000 Strasbourg, France. EM wissalselmi@yahoo.fr FU Institution de la Recherche et de l'Enseignement Superieur Agricoles (IRESA-Tunisia); 'Zone Atelier Environnementale Strasbourg' (ZAEU); "Eurometropole de Strasbourg" (EMS) FX This work was supported by "Institution de la Recherche et de l'Enseignement Superieur Agricoles (IRESA-Tunisia), 'Zone Atelier Environnementale Strasbourg' (ZAEU) and "Eurometropole de Strasbourg" (EMS). We thank ASPA for air pollution concentration data; Dr. George Najjar for advice and Bob Hoehn, Satoshi Hirabayashi, Allison Bodine and all the staff of USDA Forest Service, Northern Research station in Syracuse, NY for assistance and data processing. NR 71 TC 3 Z9 3 U1 28 U2 28 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 1618-8667 J9 URBAN FOR URBAN GREE JI Urban For. Urban Green. PD JUN 1 PY 2016 VL 17 BP 192 EP 201 DI 10.1016/j.ufug.2016.04.010 PG 10 WC Plant Sciences; Environmental Studies; Forestry; Urban Studies SC Plant Sciences; Environmental Sciences & Ecology; Forestry; Urban Studies GA DY2HL UT WOS:000384913600020 ER PT J AU Chang, CL AF Chang, Chiou Ling TI Proteomics/qPCR protocol to estimate physical ages of wild male oriental fruit flies, Bactrocera dorsalis (Hendel) SO JOURNAL OF ASIA-PACIFIC ENTOMOLOGY LA English DT Review DE Odorant binding protein; Protein analysis; Bactrocera dorsalis ID INFLUENCES PROTEIN EXPRESSION; FLY; DROSOPHILA; TEPHRITIDAE; LATIFRONS; LUFENURON; DIPTERA; ADULTS AB Laboratory reared fruit flies may have different behavioral phenotypes as compared to the wild in the field. The domesticated lab setting and the absence of factors from the natural environment on lab reared insects may result in different behavioral patterns, such as the length of life cycle. This study was performed to conduct a regression analysis of the ratio of odorant binding protein 99b (OBP99b) expression from 4 to 12-d-old oriental fruit fly males, relative to 1-d-old males to estimate the physical ages of wild male fruit flies. We established two polynomial linear regression equations based on 4-12 days old lab-reared flies using proteomics and qPCR. The equations are Y = -0.7768 + 0.7205X, R-2 = 0.89 (for proteomics) and Y = -0.6478 + 0344X, R-2 = 0.64 (for qPCR). We used these equations to estimate the physical ages of wild-caught male fruit flies. These results indicate that despite multiple behavioral differences between laboratory reared and field-caught flies, the physical ages of both groups are identical. We suggest proteomics and qPCR analysis of selected genes and the proteins they encode may be developed into reliable tools for determining the ages of wild-caught animals, including oriental fruit flies. Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. C1 [Chang, Chiou Ling] USDA ARS, Daniel K Inouye US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. RP Chang, CL (reprint author), USDA ARS, Daniel K Inouye US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. EM stella.chang@ars.usda.gov NR 17 TC 0 Z9 0 U1 4 U2 4 PU KOREAN SOC APPLIED ENTOMOLOGY PI SUWON PA NATL INST AGRICULTURAL SCIENCE & TECHNOLOGY, DIVISION ENTOMOLOGY, RDA, 249 SEODUN-DONG, SUWON, 441-707, SOUTH KOREA SN 1226-8615 EI 1876-7990 J9 J ASIA-PAC ENTOMOL JI J. Asia-Pac. Entomol. PD JUN PY 2016 VL 19 IS 2 BP 347 EP 351 DI 10.1016/j.aspen.2016.03.012 PG 5 WC Entomology SC Entomology GA DX6TB UT WOS:000384514900014 ER PT J AU Dunlap, CA Bowman, MJ Schisler, DA Rooney, AP AF Dunlap, Christopher A. Bowman, Michael J. Schisler, David A. Rooney, Alejandro P. TI Genome analysis shows Bacillus axarquiensis is not a later heterotypic synonym of Bacillus mojavensis; reclassification of Bacillus malacitensis and Brevibacterium halotolerans as heterotypic synonyms of Bacillus axarquiensis SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID PHYLOGENOMIC ANALYSIS SHOWS; DNA-DNA HYBRIDIZATION; SP-NOV; SEQUENCE; SUBTILIS; BACTERIA; NECROSIS; PROTEIN AB Bacillus axarquiensis and Bacillus malacitensis were previously reported to be later heterotypic synonyms of Bacillus mojavensis, based primarily on DNA-DNA relatedness values. We have sequenced draft genomes of Bacillus axarquiensis NRRL B-41617(T) and Bacillus malacitensis NRRL B-41618(T). Comparative genomics and DNA-DNA relatedness calculations showed that while Bacillus axarquiensis and Bacillus malacitensis are synonymous with each other, they are not synonymous with Bacillus mojavensis. In addition, a draft genome was completed for Brevibacterium halotolerans, a strain long suspected of being a Bacillus subtilis group member based on 16S rRNA similarities (99.8 % with Bacillus mojavensis). Comparative genomics and DNA DNA relatedness calculations showed that Brevibacterium halotolerans is synonymous with Bacillus axarquiensis and Bacillus malacitensis. The pairwise in silico DNA-DNA hybridization values calculated in comparisons between the three conspecific strains were all greater than 92%, which is well above the standard species threshold of 70%. While the pairwise in silico DNA-DNA hybridization values calculated in comparisons of the three conspecific strains with Bacillus mojavensis were all less than 65%. The combined results of our genotype and phenotype studies showed that Bacillus axarquiensis, Bacillus malacitensis and Brevibacterium halotolerans are conspecific and distinct from Bacillus mojavensis. Because the valid publication of the name Bacillus axarquiensis predates the publication of the name Bacillus malacitensis, we propose that Bacillus malacitensis be reclassified as a synonym of Bacillus axarquiensis. In addition, we propose to reclassify Brevibacterium halotolerans as a synonym of Bacillus axarquiensis. An amended description of Bacillus axarquiensis is provided. C1 [Dunlap, Christopher A.; Schisler, David A.; Rooney, Alejandro P.] ARS, Crop Bioprotect Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Bowman, Michael J.] ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL USA. RP Dunlap, CA (reprint author), ARS, Crop Bioprotect Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. EM christopher.dunlap@ars.usda.gov NR 26 TC 1 Z9 1 U1 3 U2 3 PU MICROBIOLOGY SOC PI LONDON PA CHARLES DARWIN HOUSE, 12 ROGER ST, LONDON WC1N 2JU, ERKS, ENGLAND SN 1466-5026 EI 1466-5034 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD JUN PY 2016 VL 66 BP 2438 EP 2443 DI 10.1099/ijsem.0.001048 PN 6 PG 6 WC Microbiology SC Microbiology GA DV4OO UT WOS:000382905100048 PM 27030978 ER PT J AU Labeda, DP Rong, XY Huang, Y Doroghazi, JR Ju, KS Metcalf, WW AF Labeda, David P. Rong, Xiaoying Huang, Ying Doroghazi, James R. Ju, Kou-San Metcalf, William W. TI Taxonomic evaluation of species in the Streptomyces hirsutus clade using multi-locus sequence analysis and proposals to reclassify several species in this clade SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID DNA-DNA HYBRIDIZATION; SUBSTITUTIONS; SYSTEMATICS AB Previous phylogenetic analysis of species of the genus Streptomyces based on 16S rRNA gene sequences resulted in a statistically well-supported Glade (100 % bootstrap value) containing eight species that exhibited very similar gross morphology in producing open looped (Retinaculum Apertum) to spiral (Spira) chains of spiny- to hairysurfaced, dark green spores on their aerial mycelium. The type strains of the species in this Glade, specifically Streptomyces bambergiensis, Streptomyces cyanoalbus, Streptomyces emeiensis, Streptomyces hirsutus, Streptomyces prasinopilosus and Streptomyces prasinus, were subjected to multi-locus sequence analysis (MLSA) utilizing partial sequences of the housekeeping genes atpD, gyrB, recA, rpoB and trpB to clarify their taxonomic status. The type strains of several recently described species with similar gross morphology, including Streptomyces chlorus, Streptomyces herbaceus, Streptomyces incanus, Streptomyces pratens and Streptomyces viridis, were also studied along with six unidentified green-spored Streptomyces strains from the ARS Culture Collection. The MLSAs suggest that three of the species under study (S. bambergiensis, S. cyanoalbus and S. emeiensis) represent synonyms of other previously described species (S. prasinus, S. hirsutus and S. prasinopilosus, respectively). These relationships were confirmed through determination of in silico DNA-DNA hybridization estimates based on draft genome sequences. The five recently described species appear to be phylogenetically distinct but the unidentified strains from the ARS Culture Collection could be identified as representatives of S. hirsutus, S. prasinopilosus or S. prasinus. C1 [Labeda, David P.] ARS, Mycotoxin Prevent & Appl Microbiol Res, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Rong, Xiaoying; Huang, Ying] Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China. [Doroghazi, James R.; Ju, Kou-San; Metcalf, William W.] Univ Illinois Champaign Urbana, Dept Microbiol, Urbana, IL USA. [Doroghazi, James R.; Ju, Kou-San; Metcalf, William W.] Univ Illinois Champaign Urbana, Inst Genom Biol, Urbana, IL USA. RP Labeda, DP (reprint author), ARS, Mycotoxin Prevent & Appl Microbiol Res, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. EM david.labeda@ars.usda.gov FU ARS National Program [301]; National Institutes of Health [GM PO1 GM077596]; Institute for Genomic Biology Postdoctoral Fellowship; NIH NRSA Postdoctoral Fellowship [GM100658] FX The able technical assistance of E. Basehoar in determining the housekeeping gene sequences and A. McGovern in determining draft genome sequences is gratefully acknowledged. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. D.P.L. and the ARS Culture Collection CRIS project was supported by ARS National Program 301. W.W.M. was supported by the National Institutes of Health (GM PO1 GM077596). J.R.D. was funded by an Institute for Genomic Biology Postdoctoral Fellowship. K.S.J. was funded by an NIH NRSA Postdoctoral Fellowship (GM100658). NR 20 TC 0 Z9 0 U1 2 U2 2 PU MICROBIOLOGY SOC PI LONDON PA CHARLES DARWIN HOUSE, 12 ROGER ST, LONDON WC1N 2JU, ERKS, ENGLAND SN 1466-5026 EI 1466-5034 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD JUN PY 2016 VL 66 BP 2444 EP 2450 DI 10.1099/ijsem.0.001017 PN 6 PG 7 WC Microbiology SC Microbiology GA DV4OO UT WOS:000382905100049 PM 26971011 ER PT J AU Kaya, E Souza, FVD Ceylan, M Jenderek, MM AF Kaya, Ergun Duerte Souza, Fernanda Vidiga Ceylan, Muammer Jenderek, Maria M. TI Conservation of Musa sp Seeds Via Cryopreservation. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Kaya, Ergun; Ceylan, Muammer] Mugla Sitki Kocman Univ, Fac Sci, Mol Biol & Genet Dept, TR-48000 Kotekli Mugla, Turkey. [Duerte Souza, Fernanda Vidiga] Embrapa Cassava & Fruits, Caixa Postal 007, BR-44380000 Cruz Das Almas, BA, Brazil. [Jenderek, Maria M.] USDA ARS, Natl Lab Genet Resources Preservat, Ft Collins, CO USA. EM ergunkaya@mu.edu.tr RI Taylor, Nigel/M-2851-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2016 VL 52 SU 1 BP S38 EP S38 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA DN2XR UT WOS:000376926800095 ER PT J AU Bruez, E Baumgartner, K Bastien, S Travadon, R Guerin-Dubrana, L Rey, P AF Bruez, E. Baumgartner, K. Bastien, S. Travadon, R. Guerin-Dubrana, L. Rey, P. TI Various fungal communities colonise the functional wood tissues of old grapevines externally free from grapevine trunk disease symptoms SO AUSTRALIAN JOURNAL OF GRAPE AND WINE RESEARCH LA English DT Article DE fingerprinting method; fungal community; grapevine trunk disease; grapevine wood tissue; pathogenic fungi; potentially beneficial fungi ID ESCA-DISEASE; PHAEOMONIELLA-CHLAMYDOSPORA; TRICHODERMA-HARZIANUM; EUTYPA DIEBACK; ENDOPHYTES; DIVERSITY; IDENTIFICATION; NURSERIES; BIODIVERSITY; CALIFORNIA AB Background and Aims: The objective of this study was to analyse the various fungal communities that colonise functional wood tissues of old vines that did not express symptoms of grapevine trunk diseases (i.e. Esca and Eutypa dieback) in the year of sampling. Plants of the cultivar Baco Blanc a grapevine hybrid of Folle Blanche and Noah used to produce Armagnac in France were sampled. Methods and Results: Forty-two and 58-year-old vines, planted in the same vineyard, were uprooted, cut longitudinally and their functional wood tissues sampled. Culture-dependent and single-strand conformational polymorphism methods were used to compare the fungal communities colonising these wood tissues. It was shown that the fungal communities were significantly different depending on the age of the grapevines. A total of 421 fungal strains were isolated and identified by internal transcribed spacer region sequencing. Conclusions: Many grapevine trunk diseases fungal pathogens, particularly the causal agents of Esca (42-year-old vines) and Eutypa dieback (58-year-old vines), as well as numerous potentially plant-beneficial mycoparasites (e.g. Trichoderma spp.), were isolated from the functional wood tissues of old grapevines. Significance of the Study: The lack of foliar symptoms among older grapevines may reflect an 'equilibrium' among trunk fungal pathogens, mycoparasites and saprobes in the functional wood tissues of trunks. C1 [Bruez, E.; Bastien, S.; Guerin-Dubrana, L.; Rey, P.] Univ Bordeaux, Bordeaux Sci Agro, Inst Sci Vigne & Vin, Unite Mixte Rech Sante & Agroecol Vignoble 1065, F-33140 Villenave Dornon, France. [Bruez, E.; Bastien, S.; Guerin-Dubrana, L.; Rey, P.] INRA, Inst Sci Vigne & Vin, Unite Mixte Rech Sante & Agroecol Vignoble 1065, F-33140 Villenave Dornon, France. [Baumgartner, K.] ARS, USDA, Davis, CA 95616 USA. [Travadon, R.] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. RP Rey, P (reprint author), Univ Bordeaux, Bordeaux Sci Agro, Inst Sci Vigne & Vin, Unite Mixte Rech Sante & Agroecol Vignoble 1065, F-33140 Villenave Dornon, France.; Rey, P (reprint author), INRA, Inst Sci Vigne & Vin, Unite Mixte Rech Sante & Agroecol Vignoble 1065, F-33140 Villenave Dornon, France. EM prey@bordeaux.inra.fr FU Bordeaux Sciences Agro; Regional Council of Aquitaine (France); Jean Poupelain Foundation; Casdar research project [V1302] FX The experiment was financed by Bordeaux Sciences Agro, the Regional Council of Aquitaine (France), the Jean Poupelain Foundation and the French Minister for Agriculture (Casdar research project V1302 on GTDs). NR 49 TC 0 Z9 0 U1 4 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1322-7130 EI 1755-0238 J9 AUST J GRAPE WINE R JI Aust. J. Grape Wine Res. PD JUN PY 2016 VL 22 IS 2 BP 288 EP 295 DI 10.1111/ajgw.12209 PG 8 WC Food Science & Technology; Horticulture SC Food Science & Technology; Agriculture GA DW0XY UT WOS:000383367600016 ER PT J AU Allen, DW Borchers, A AF Allen, Douglas W. Borchers, Allison TI Conservation Practices and the Growth of US Cash Rent Leases SO JOURNAL OF AGRICULTURAL ECONOMICS LA English DT Article DE Conservation; institutional economics; land use and tenure; organic agriculture; Property rights; soil degradation; United States ID LAND-TENURE; CONTRACTS; AGRICULTURE; FARMLAND AB Over the past 20 years the ratio of cash rent to cropshare land leases across the US has more than doubled. We test different theories that might explain this, and conclude that the shift is mostly the result of revolutionary changes in cultivation practices. The switch from conventional to conservation tillage brought about by changes in herbicide technologies, genetically modified seeds, increased fuel costs, and knowledge of the benefits of soil micro-organisms, has reduced a tenant-farmer's ability to exploit a landowner's soil. This removes a major incentive to cropshare and makes cash renting more attractive. Using USDA field-level data from across the US, we find strong support for this hypothesis, and some evidence that increased corporate structure also influences cash renting. Alternatively, we cannot find evidence that changes in risk, risk-aversion or insurance coverage explain the growth in cash renting. C1 [Allen, Douglas W.] Simon Fraser Univ, Dept Econ, Econ, Burnaby, BC, Canada. [Borchers, Allison] USDA, Econ Res Serv, Washington, DC 20250 USA. RP Allen, DW (reprint author), Simon Fraser Univ, Dept Econ, Econ, Burnaby, BC, Canada. EM allen@sfu.ca NR 23 TC 0 Z9 0 U1 4 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-857X EI 1477-9552 J9 J AGR ECON JI J. Agric. Econ. PD JUN PY 2016 VL 67 IS 2 BP 491 EP 509 DI 10.1111/1477-9552.12153 PG 19 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA DW0RY UT WOS:000383351000012 ER PT J AU Kao, M Columbus, DA Suryawan, A Steinhoff-Wagner, J Hernandez-Garcia, A Nguyen, HV Fiorotto, ML Davis, TA AF Kao, Michelle Columbus, Daniel A. Suryawan, Agus Steinhoff-Wagner, Julia Hernandez-Garcia, Adriana Nguyen, Hanh V. Fiorotto, Marta L. Davis, Teresa A. TI Enteral beta-hydroxy-beta-methylbutyrate supplementation increases protein synthesis in skeletal muscle of neonatal pigs SO AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM LA English DT Article DE amino acid; protein metabolism; translation initiation; infant ID LOW-BIRTH-WEIGHT; AMINO-ACID AVAILABILITY; TRANSLATION INITIATION; SATELLITE CELL; LEUCINE SUPPLEMENTATION; ALPHA-KETOISOCAPROATE; HMB SUPPLEMENTATION; UBIQUITIN LIGASES; MAMMALIAN TARGET; MTOR PATHWAY AB Many low-birth weight infants are at risk for poor growth due to an inability to achieve adequate protein intake. Administration of the amino acid leucine stimulates protein synthesis in skeletal muscle of neonates. To determine the effects of enteral supplementation of the leucine metabolite beta-hydroxy-beta-methylbutyrate (HMB) on protein synthesis and the regulation of translation initiation and degradation pathways, overnight-fasted neonatal pigs were studied immediately (F) or fed one of five diets for 24 h: low-protein (LP), high-protein (HP), or LP diet supplemented with 4 (HMB4), 40 (HMB40), or 80 (HMB80) mu mol HMB.kg body wt(-1).day(-1). Cell replication was assessed from nuclear incorporation of BrdU in the longissimus dorsi (LD) muscle and jejunum crypt cells. Protein synthesis rates in LD, gastrocnemius, rhomboideus, and diaphragm muscles, lung, and brain were greater in HMB80 and HP and in brain were greater in HMB40 compared with LP and F groups. Formation of the eIF4E.eIF4G complex and S6K1 and 4E-BP1 phosphorylation in LD, gastrocnemius, and rhomboideus muscles were greater in HMB80 and HP than in LP and F groups. Phosphorylation of eIF2 alpha and eEF2 and expression of SNAT2, LAT1, MuRF1, atrogin-1, and LC3-II were unchanged. Numbers of BrdU-positive myonuclei in the LD were greater in HMB80 and HP than in the LP and F groups; there were no differences in jejunum. The results suggest that enteral supplementation with HMB increases skeletal muscle protein anabolism in neonates by stimulation of protein synthesis and satellite cell proliferation. C1 [Kao, Michelle; Columbus, Daniel A.; Suryawan, Agus; Steinhoff-Wagner, Julia; Hernandez-Garcia, Adriana; Nguyen, Hanh V.; Fiorotto, Marta L.; Davis, Teresa A.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. RP Davis, TA (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM tdavis@bcm.edu FU National Institute of Child Health and Human Development Grant [HD-072891]; US Department of Agriculture (USDA)/National Institute of Food and Agriculture Grant [2013-67015-20438]; National Institute of Arthritis and Musculoskeletal and Skin Diseases Grants [AR-044474, AR-046308]; USDA Current Research Information System Grant [6250-51000-055]; Abbott Nutrition FX This project was supported by Abbott Nutrition, National Institute of Child Health and Human Development Grant HD-072891, US Department of Agriculture (USDA)/National Institute of Food and Agriculture Grant 2013-67015-20438, National Institute of Arthritis and Musculoskeletal and Skin Diseases Grants AR-044474 and AR-046308, and USDA Current Research Information System Grant 6250-51000-055. This work is a publication of the USDA/Agricultural Research Service Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, and Abbott Nutrition. The contents of this publication do not necessarily reflect the views or politics of the USDA, nor does the mention of trade names, commercial products, or organizations imply endorsements by the US Government. NR 83 TC 1 Z9 1 U1 2 U2 2 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1849 EI 1522-1555 J9 AM J PHYSIOL-ENDOC M JI Am. J. Physiol.-Endocrinol. Metab. PD JUN 1 PY 2016 VL 310 IS 11 BP E1072 EP E1084 DI 10.1152/ajpendo.00520.2015 PG 13 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA DV9TV UT WOS:000383286000008 PM 27143558 ER PT J AU Vreysen, MJB Klassen, W Carpenter, JE AF Vreysen, Marc J. B. Klassen, Waldemar Carpenter, James E. TI Overview of technological advances toward greater efficiency and efficacy in sterile insect-inherited sterility programs against moth pests SO FLORIDA ENTOMOLOGIST LA English DT Article DE biological control; colonization; competitiveness; mass-rearing; irradiation; dispersal; field performance; mating status; modeling; mating; compatibility; mating competitiveness; mating status; flight ability; cold adaptation; cage studies; open field studies; female attractant; host-derived kairomone; conspecific larvae-infested host; marking; natural isotope signature ID LINNAEUS LEPIDOPTERA-TORTRICIDAE; FLY DIPTERA TEPHRITIDAE; FEMALE CODLING MOTH; BROWN APPLE MOTHS; CYDIA-POMONELLA; THERMAL TOLERANCE; CACTOBLASTIS-CACTORUM; ELDANA-SACCHARINA; RADIATION BIOLOGY; ISOTOPE MARKING AB Lepidopteran species are amongst the most damaging pests of food and fiber crops world-wide. Pest lepidopterans are often managed injudiciously by spraying crops with large amounts of broad-spectrum-and often-persistent insecticides. In view of increased occurrence of resistance against these insecticides, and their negative impacts on the environment and ecosystems, the need for control tactics that are not only effective but also friendlier to the environment is becoming more and more pressing. Both the sterile insect technique (SIT), and the related inherited sterility (IS) technique offer great potential as additional control tactics for integration with other control methods in area-wide integrated pest management approaches against lepidopteran pests. However the SIT/IS can only be applied successfully when the released sterile insects can effectively compete with their wild counterparts for mating with wild females. Although there are a number of programs where the SIT has been used very effectively against key lepidopteran pests, there is great potential for further expansion of the SIT/IS technology to target other key lepidopteran pests, or to improve the SIT/IS for already targeted Lepidoptera. Such expansion could be facilitated through improvements of mass-rearing, measurement and control of quality, handling, irradiation, shipping, release and field assessment technologies that would increase program efficiency and efficacy. To foster such advances, the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, implemented a Coordinated Research Project (CRP) from 2008 to 2014 entitled "Increasing the efficiency of Lepidoptera SIT by enhanced quality control". This Project organized and sponsored research and development on the following key objectives: (1) identify and investigate factors and variables affecting the quality of the produced and released insects and their field performance, (2) identify and develop new tools and methods to assess and predict the field performance of sterile insects, and (3) improve the artificial rearing of several moth species through a better understanding and management of genetic resources. The key research outputs of the CRP are summarized in this paper. C1 [Vreysen, Marc J. B.] IAEA, Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Lab, Wagramerstr 5, A-1400 Vienna, Austria. [Klassen, Waldemar] Univ Florida, Trop Res & Educ Ctr, 18905 SW 280th St, Homestead, FL 33031 USA. [Carpenter, James E.] ARS, Crop Protect & Management Res Unit, USDA, Tifton, GA 31794 USA. RP Vreysen, MJB (reprint author), IAEA, Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Lab, Wagramerstr 5, A-1400 Vienna, Austria. EM m.vreysen@iaea.org NR 106 TC 0 Z9 0 U1 1 U2 1 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2016 VL 99 SI 1 BP 1 EP 12 PG 12 WC Entomology SC Entomology GA DU5JZ UT WOS:000382249300001 ER PT J AU Mudavanhu, P Addison, P Carpenter, JE Conlong, DE AF Mudavanhu, Pride Addison, Pia Carpenter, James E. Conlong, Des E. TI Mating compatibility and competitiveness between wild and laboratory strains of Eldana saccharina (Lepidoptera: Pyralidae) after radiation treatment SO FLORIDA ENTOMOLOGIST LA English DT Article DE African sugarcane stalk borer; sterile insect technique; mating behavior; mating indices; sexual selection; performance ID STERILE INSECT TECHNIQUE; FIELD CAGE CONDITIONS; CERATITIS-CAPITATA; MEDFLY DIPTERA; FRUIT-FLY; SEXUAL COMPATIBILITY; INHERITED STERILITY; MOTH LEPIDOPTERA; TEPHRITIDAE; PERFORMANCE AB The efficacy of the sterile insect technique (SIT) applied as part of area-wide integrated pest management (AW-IPM) depends on efficient transfer of sperm carrying dominant lethal mutations from sterile males to wild females. Success or failure of this strategy is therefore critically dependent on quality and ability of sterile males to search for and copulate with wild females. The African sugarcane borer, Eldana saccharina Walker (Lepidoptera: Pyralidae) is an economic pest of sugarcane targeted for control in South Africa using an AW-IPM approach with a SIT component. As part of further steps towards development of the technique, levels of mating competitiveness and compatibility were assessed by observing the extent to which individuals from different populations interbreed when confined together under both laboratory and semi-field conditions. Three types of pair-wise competition experiments were conducted: non-irradiated laboratory adults vs. non-irradiated wild adults, irradiated (200 Gy) laboratory adults vs. non-irradiated wild adults, and non-irradiated laboratory adults vs. irradiated (200 Gy) laboratory adults. Data from these tests were used to generate indices for mating performance and measuring sexual compatibility between strains. Irrespective of trial location, wild moths did not discriminate against irradiated or laboratory-reared moths, indicating no negative effects on acceptability for mating due to laboratory rearing or radiation treatment. In general, irradiated males mated significantly more than their wild counterparts regardless of the type of female, which indicated that they were still as competitive as their wild counterparts. The mating indices generated showed no evidence of incipient pre-mating isolation barriers or sexual incompatibility with the wild strain. Data presented in this paper therefore indicate that there is scope for further development of the SIT as an addition to the arsenal of tactics available for AW-IPM of this economic pest. C1 [Mudavanhu, Pride] ARC, PPRI, Vredenburg Campus,P Bag X5017, ZA-7599 Stellenbosch, South Africa. [Mudavanhu, Pride; Addison, Pia; Conlong, Des E.] Univ Stellenbosch, Conservat Ecol & Entomol Dept, P Bag X1, ZA-7602 Matieland, South Africa. [Carpenter, James E.] ARS, USDA, CPMRU, Tifton, GA 31794 USA. [Conlong, Des E.] SASRI, 170 Flanders Dr, ZA-4300 Durban, South Africa. RP Mudavanhu, P (reprint author), ARC, PPRI, Vredenburg Campus,P Bag X5017, ZA-7599 Stellenbosch, South Africa.; Mudavanhu, P (reprint author), Univ Stellenbosch, Conservat Ecol & Entomol Dept, P Bag X1, ZA-7602 Matieland, South Africa. EM prideshadreck@gmail.com FU South African Sugarcane Research Institute (SASRI) FX This work was part of the FAO/IAEA Coordinated Research Project on Increasing the Efficiency of Lepidoptera SIT by Enhanced Quality Control. The research was funded and logistically supported by the South African Sugarcane Research Institute (SASRI). We are sincerely grateful to staff at the SASRI Entomology department IRU for logistical support and providing all the insects used for these experiments. We thank SIT-Africa Pty (Ltd) for conducting the radiation treatments on the test insects used in this study. We also thank Tom Blomefield (Agricultural Research Council: Plant Protection Research Institute, Infruitec, Stellenbosch) for supplying the field cage, and Stellenbosch University ConsEnt Department for providing facilities to conduct the assays. NR 53 TC 2 Z9 2 U1 1 U2 1 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2016 VL 99 SI 1 BP 54 EP 65 PG 12 WC Entomology SC Entomology GA DU5JZ UT WOS:000382249300007 ER PT J AU Lopez-Martinez, G Carpenter, JE Hight, SD Hahn, DA AF Lopez-Martinez, Giancarlo Carpenter, James E. Hight, Stephen D. Hahn, Daniel A. TI Anoxia-conditioning hormesis alters the relationship between irradiation doses for survival and sterility in the cactus moth, Cactoblastis cactorum (Lepidoptera: Pyralidae) SO FLORIDA ENTOMOLOGIST LA English DT Article DE sterile insect technique; dose response; invasive species; inherited (F-1) sterility ID FRUIT-FLY DIPTERA; GAMMA-IRRADIATION; SEXUAL COMPETITIVENESS; F-1 STERILITY; CODLING MOTH; TEPHRITIDAE; NITROGEN; MALES; PUPAE; PERFORMANCE AB One of the most important components of a program that has a sterile insect technique (SIT) component is an appropriate irradiation dose. Knowing the organismal dose-response enables the selection of a dose that induces the highest level of sterility while preserving the sexual competitiveness and other desired qualities of the sterile insect. Finding this balance in Lepidoptera is crucial because of the use of inherited (F-1) sterility, where the irradiated parent must be competitive enough to mate while its offspring must be sterile. Manipulations of atmospheric oxygen content have been shown to be an effective way of lowering post-irradiation somatic damage while preserving sterility and improving sterile insect performance, particularly in fruit flies. In this study we tested the irradiation dose response of adults of the cactus moth, Cactoblastis cactorum (Berg) (Lepidoptera: Pyralidae), and the effects of those doses on sterility, longevity, and F-1 performance, and whether a nitrogen conditioning-treatment (anoxia) prior to and during irradiation affected these metrics. We found that male and female fecundities were not impacted by dose or atmospheric treatment, but females were sterilized at lower doses than males. Eggs of irradiated parents took longer to hatch than those of unirradiated controls, and offspring of moths irradiated in anoxia lived longer in the absence of food and water. Anoxia conditioning rescued female fertility at intermediate doses but had no similar rescue effect on male fertility, which was always greater than female fertility at a given dose. Males generally lived longer than females and anoxia had a strong effect in lowering the male mortality rate and extending lifespan at a given dose. We show evidence that anoxia-conditioning prior to and during irradiation as part of a lepidopteran program with an SIT component could improve parental and larval performance and longevity. C1 [Lopez-Martinez, Giancarlo; Hahn, Daniel A.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Lopez-Martinez, Giancarlo] New Mexico State Univ, Dept Biol, Las Cruces, NM 88003 USA. [Carpenter, James E.] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. [Hight, Stephen D.] Florida A&M Univ, USDA ARS CMAVE, Tallahassee, FL 32308 USA. RP Lopez-Martinez, G (reprint author), Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. EM gclopez@nmsu.edu OI Lopez-Martinez, Giancarlo/0000-0002-7937-5002 FU USDA-NIFA [2011-67012-30671]; USDA-APHIS [CA-12-8130-0159] FX This work was part of the FAO/IAEA Coordinated Research Project on Increasing the Efficiency of Lepidoptera SIT by Enhanced Quality Control. This research was funded by USDA-NIFA 2011-67012-30671 to GLM and USDA-APHIS CA-12-8130-0159 to DAH. The authors wish to thank George Schneider, Suzanne Fraser, and Carl Gillis from the Florida Department of Agriculture and Consumer Services (FDACS-DPI) for providing irradiation assistance and expertize, and Robert Caldwell and Susan Drawdy from the USDA-ARS Crop Protection and Management Research Unit in Tifton, Georgia for providing us with cactus moths and transporting them from Tifton to Gainesville. Additionally we thank Sabrina A. White and Theodore R. Cogley for all their help setting up experiments, and monitoring longevity and egg hatch. This work also received substantial intellectual support from the FAO/IAEA Coordinated Research Project. NR 43 TC 1 Z9 1 U1 1 U2 1 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2016 VL 99 SI 1 BP 95 EP 104 PG 10 WC Entomology SC Entomology GA DU5JZ UT WOS:000382249300012 ER PT J AU Hood-Nowotny, R Harari, A Seth, RK Wee, SL Conlong, DE Suckling, DM Woods, B Lebdi-Grissa, K Simmons, G Carpenter, JE AF Hood-Nowotny, Rebecca Harari, Ally Seth, Rakesh K. Wee, Suk Ling Conlong, Des E. Suckling, David M. Woods, Bill Lebdi-Grissa, Kaouthar Simmons, Gregory Carpenter, James E. TI Stable isotope markers differentiate between mass-reared and wild Lepidoptera in sterile insect technique programs SO FLORIDA ENTOMOLOGIST LA English DT Article DE elemental analysis-isotope ratio mass-spectrometry (EA-IRMS); sterile insects; release/recapture; isotopic signature; sugar; larval diet; markers; moths ID CACTOBLASTIS-CACTORUM LEPIDOPTERA; BROWN APPLE MOTHS; INHERITED STERILITY; ARTIFICIAL DIET; RELEASE PROGRAM; TORTRICIDAE; PYRALIDAE; MANAGEMENT; REPRODUCTION; SUPPRESSION AB In this study we identified a number of moth (Lepidoptera) species that are potential targets for the sterile insect technique (SIT), and we assessed the feasibility of using stable isotope signatures as markers to distinguish mass-reared from wild moth species. Large natural differences in the isotopic signatures of commercially available sugars render them novel markers for mass-reared insects. Sugar beet (Beta vulgaris L.; Caryophyllales: Amaranthaceae), a C-3 plant, has a stable isotopic signature (a measure of the ratio of the stable isotopes C-13:C-12) of around -27% relative to Vienna Pee Dee Belemnite (VPDB; the international C isotope standard for the stable isotopes, C-13 and C-12), and sugarcane (Saccharum spp.; Poales: Poaceae), a C-4 plant, has an isotopic signature of around -11%. Thus by means of such a distinct isotope ratio in the sugar in the diet, mass-reared insects can be easily distinguished from wild insects with a high degree of certainty. It was shown that the method could be extended using a multiple isotope approach, with N-15 or a full suite of C, N, S and O isotopes. Intrinsic isotope marking of mass-reared moths proved to be an accurate means of distinguishing wild from mass-reared populations, based on isotopic differences between the wild host plant species and the diets used in mass-rearing, which where possible, had been manipulated to contain the isotopically divergent sugar type. This intrinsic labeling using stable isotopes could be useful in the assessment of the quality of mass-reared moths, because a stable isotope is a marker that does not affect the insect in any detrimental manner. C1 [Hood-Nowotny, Rebecca] Austrian Inst Technol, Vienna, Austria. [Hood-Nowotny, Rebecca] Univ Vienna, Fac Life Sci, Dept Chem Ecol & Ecosyst Res, Althanstr 14, A-1090 Vienna, Austria. [Hood-Nowotny, Rebecca] Austrian Inst Technol GmbH, AIT, Konrad Lorenz Str 24, A-3430 Tulln, Austria. [Harari, Ally] Minist Agr & Rural Dev, Agr Res Org, Volcani Ctr, Dept Entomol, POB 6, IL-50250 Bet Dagan, Israel. [Seth, Rakesh K.] Univ Delhi, Dept Zool, New Delhi 110007, India. [Wee, Suk Ling] Univ Kebangsaan Malaysia, Sch Environm & Nat Resources Sci, Fac Sci & Technol, Bangi 43600, Selangor Darul, Malaysia. [Conlong, Des E.] SASRI, 170 Flanders Dr, ZA-4300 Mt Edgecombe, South Africa. [Conlong, Des E.] Univ Stellenbosch, Dept Conservat Ecol & Entomol, Private Bag X01, Matieland, South Africa. [Suckling, David M.] New Zealand Inst Plant & Food Res Ltd, Hort & Food Inst, Mt Albert Res Ctr, 120 Mt Albert Rd,Private Bag 92169,PB 4704, Canterbury, New Zealand. [Woods, Bill] Dept Agr & Food, 3 Baron Hay Ct, S Perth, WA 6151, Australia. [Lebdi-Grissa, Kaouthar] Inst Natl Agron Tunisie, 43 Ave Charles Nicole, Tunis 1082, Tunisia. [Simmons, Gregory] USDA APHIS PPQ CPHST, 1636 E Alisal, Salinas, CA 93905 USA. [Carpenter, James E.] USDA ARS CPMRU, 2747 Davis Rd, Tifton, GA 31794 USA. RP Hood-Nowotny, R (reprint author), Austrian Inst Technol, Vienna, Austria.; Hood-Nowotny, R (reprint author), Univ Vienna, Fac Life Sci, Dept Chem Ecol & Ecosyst Res, Althanstr 14, A-1090 Vienna, Austria.; Hood-Nowotny, R (reprint author), Austrian Inst Technol GmbH, AIT, Konrad Lorenz Str 24, A-3430 Tulln, Austria. EM Hood.fl@AIT.ac.at OI Suckling, David/0000-0001-7216-9348 NR 64 TC 2 Z9 2 U1 6 U2 6 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2016 VL 99 SI 1 BP 166 EP 176 PG 11 WC Entomology SC Entomology GA DU5JZ UT WOS:000382249300019 ER PT J AU Hood-Nowotny, R Mayr, L Saad, N Seth, RK Davidowitz, G Simmons, G AF Hood-Nowotny, Rebecca Mayr, Leo Saad, Nabil Seth, Rakesh K. Davidowitz, Goggy Simmons, Gregory TI Towards incorporating insect isotope analysis using cavity ring-down spectroscopy into area-wide insect pest management programs SO FLORIDA ENTOMOLOGIST LA English DT Article DE marking; labelling; enrichment; natural abundance; mass spectrometry; nuclear application; laser; Lepidoptera; moths ID FRACTIONATION; DISPERSAL; MOSQUITOS; MARKING; C-13 AB Measurement of natural stable isotope signatures of insects has proven to be a useful method in determining their natal origin, feeding strategies and mating behavior, and has spawned a new discipline of insect isotope forensics. However the complexity of measuring isotope signatures using elemental analysis-isotope ratio mass-spectrometry (EA-IRMS) has encountered significant technical barriers, which together with costs, have hindered the advancement and wide spread adoption of this potentially useful approach. In the last decade simpler technologies to measure stable isotope ratios in samples based on laser spectroscopy have been developed. We foresee that these instruments will fuel the advancement and adoption of insect isotope forensics into pest management programs because they are easy to use, more affordable, and complement existing marking tools. However prior to the adoption of these new technologies it is necessary to test their fitness for purpose and explore their limitations. In this paper we set out to test the suitability of using combustion module-cavity ring-down spectroscopy (CM-CRDS) for measuring isotope ratios in an entomological context, covering marking of insects, mating behavior and dietary assessment. We carried out a series of experiments to compare the isotope measurements made with the CM-CRDS with the traditional method of EA-IRMS. Initially we compared measurements of carbon isotope values of a variety of common diet components used in insect rearing, which covered a wide range of carbon isotope values. There was good agreement in the isotope values obtained, using the CM-CRDS and the EA-IRMS with similar accuracy and precision. Secondly we compared the 2 methods to measure carbon isotope values of the common cutworm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) samples, and again there was good agreement in the values obtained. Thirdly we tested whether the CM-CRDS could be used to determine the paternity of spermataphores, based on the differential isotope signatures of the paternal and maternal moths, as a result of differences in their rearing diets. The CM-CRDS proved to be fit for the purpose of measuring isotope values in the spermataphores of common cutworm and suggested that the CM-CRDS technology would be suitable for these isotope based mating studies in moths if the spermatophores were of sufficient mass. Finally we explored a number of the issues surrounding CM-CRDS and isotope technologies in general, e.g., logistical considerations, economics of operation, sample size, etc. We conclude that CM-CRDS is a suitable instrument for measuring stable isotope carbon signatures in moths, and most probably other sufficiently large insects and that CM-CRDS could be easily used in both operational and research contexts. C1 [Hood-Nowotny, Rebecca] Environm Resources & Technol AIT Austrian Inst Te, Hlth & Environm Dept, Konrad Lorenz Str 24, A-3430 Tulln, Austria. [Mayr, Leo] IAEA Labs Seibersdorf, IAEA, A-2444 Seibersdorf, Austria. [Saad, Nabil] Picarro Inc, 3105 Patrick Henry Dr, Santa Clara, CA 95054 USA. [Seth, Rakesh K.] Univ Delhi, Dept Zool, New Delhi 110007, India. [Davidowitz, Goggy] Univ Arizona, Dept Entomol, Marley 741J, Tucson, AZ 85721 USA. [Simmons, Gregory] CPHST CPHST Calif Stn, PPQ, APHIS, USDA, 1636 E Alisal St, Salinas, CA 93905 USA. RP Hood-Nowotny, R (reprint author), Environm Resources & Technol AIT Austrian Inst Te, Hlth & Environm Dept, Konrad Lorenz Str 24, A-3430 Tulln, Austria. EM Rebecca.Hood@ait.ac.at FU USDA-APHIS-PPQ [14-8130-0319-CA] FX This work was part of the FAO/IAEA Coordinated Research Project on Increasing the Efficiency of Lepidoptera SIT by Enhanced Quality Control, and was supported by USDA-APHIS-PPQ grant No. 14-8130-0319-CA "Development of stable isotopes and new biomechanical tools for identification of sterile insects and determination of pest origin." NR 25 TC 1 Z9 1 U1 2 U2 2 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2016 VL 99 SI 1 BP 177 EP 184 PG 8 WC Entomology SC Entomology GA DU5JZ UT WOS:000382249300020 ER PT J AU Hight, SD Carpenter, JE AF Hight, Stephen D. Carpenter, James E. TI Performance improvement through quality evaluations of sterile cactus moths, Cactoblastis cactorum (Lepidoptera: Pyralidae), mass-reared at two insectaries SO FLORIDA ENTOMOLOGIST LA English DT Article DE Opuntia; invasive insects; quality control; mass-rearing; sterile insect technique; SIT ID CYDIA-POMONELLA LEPIDOPTERA; LABORATORY EVALUATION; GAMMA-RADIATION; NORTH-AMERICA; MATING STATUS; TORTRICIDAE; FIELD; MOBILITY; OPUNTIA; TEMPERATURES AB A bi-national program was established by Mexico and the United States to mitigate the threat of the cactus moth, Cactoblastis cactorum (Berg) (Lepidoptera: Pyralidae)-an invasive herbivore from South America-to native Opuntia spp. (Caryophyllales: Cactaceae) biodiversity and Opuntia-based industries. Mass-rearing, sterilization, and transport and release technologies assisted with the development of several control tactics including the sterile insect technique. Following the successful eradication of C. cactorum from Mexico and the elimination of C. cactorum from Alabama barrier islands, the bi-national program established an additional mass-rearing insectary for the production of sterile moths. Laboratory and field bioassays were conducted on sterile moths from both insectaries. Bioassays and assessments included moth mass, moth longevity, percentage of female moths mated at time of collection from the insectary, percentage of female moths mated 24 h after collection, flight ability, percentage recaptured after release in the field, and mean distance dispersed from release site. Data from the quality assessments and comparisons between the 2 insectaries were used as feedback mechanisms to make protocol changes in both rearing and handling that improved sterile moth quality and performance. C1 [Hight, Stephen D.] FAMU, Ctr Biol Control, USDA ARS CMAVE, Tallahassee, FL 32308 USA. [Carpenter, James E.] USDA ARS CPMRU, Tifton, GA 31794 USA. RP Hight, SD (reprint author), FAMU, Ctr Biol Control, USDA ARS CMAVE, Tallahassee, FL 32308 USA. EM Stephen.hight@ars.usda.gov FU USDA-Mexico Bi-National Cactus Moth Program FX We thank Susan Drawdy, Robert Caldwell (USDA-ARS-CPMRU, Tifton, GA), Amy Howe, Michael Banaszek, George Schneider, and Carl Gillis (FDACS-DPI, Gainesville, FL) for technical assistance in rearing and sterilizing study moths; Chris Albanese and John Mass (USDA-ARS-CMAVE, Tallahassee, FL) for assistance with field bioassays; and the UF-IFAS farm in Citra, FL for maintenance of our cactus plantation. This research was partially funded by the USDA-Mexico Bi-National Cactus Moth Program. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. This work was part of the FAO/IAEA Coordinated Research Project on Increasing the Efficiency of Lepidoptera SIT by Enhanced Quality Control. NR 56 TC 1 Z9 1 U1 0 U2 0 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2016 VL 99 SI 1 BP 206 EP 214 PG 9 WC Entomology SC Entomology GA DU5JZ UT WOS:000382249300024 ER PT J AU Boersma, N Carpenter, JE AF Boersma, Nevill Carpenter, James E. TI Influence of holding temperature and irradiation on field performance of mass-reared Thaumatotibia leucotreta (Lepidoptera: Tortricidae) SO FLORIDA ENTOMOLOGIST LA English DT Article DE sterile insect technique; competitiveness; false codling moth; chilling; handling; quality control ID FALSE CODLING MOTH; STERILE INSECT TECHNIQUE; RADIATION; POPULATION; QUALITY AB The sterile insect technique (SIT) is as an important component to the area-wide integrated management of the false codling moth, Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae), which was successfully implemented in the Western Cape region of South Africa and subsequently expanded to citrus areas in the Eastern Cape region of South Africa. This integrated control program, which transports sterile moths from a rearing facility in Citrusdal, South Africa to orchards in both the Western and Eastern Cape, must continuously examine production, handling, processing, transport, and release protocols to ensure the delivery of high-quality sterile moths. While the use of cold temperature to immobilize moths is standard protocol for SIT programs to increase the density of moths for purposes of collecting, handling, irradiation, transport and release, some concern has been raised that rapid chilling and long cold temperature storage of moths may negatively impact field performance of some insectary-reared insects. We conducted trials to examine the effect of irradiation with 150 Gy of gamma rays and cold temperature storage on the performance of T. leucotreta moths released in citrus orchards. The radiation treatment did not significantly affect the performance of T. leucotreta moths released in citrus orchards. However, compared with moths held at room temperature, moths that were rapidly chilled were less likely to be captured in pheromone traps and less likely to disperse as great distances following release in citrus orchards. Additional research is needed to identify an alternative to rapid chilling and cold temperature storage that does not impair mating competitiveness and dispersal of irradiated T. Leucotreta adults. Procedural changes that will maintain or enhance sterile T. leucotreta moth quality and performance in the field, while allowing for the cost-effective handling and processing of the sterile moths, need to be considered. C1 [Boersma, Nevill] Xsit, POB 422, ZA-7340 Citrusdal, South Africa. [Carpenter, James E.] ARS, USDA, USDA ARS CPMRU, Tifton, GA 31794 USA. RP Boersma, N (reprint author), Xsit, POB 422, ZA-7340 Citrusdal, South Africa. EM nb@xsit.co.za FU IAEA FX This work was part of the FAO/IAEA Coordinated Research Project on Increasing the Efficiency of Lepidoptera SIT by Enhanced Quality Control. We acknowledge the IAEA for providing funds to support this research. We acknowledge Hendrik Hofmeyr for proof reading the document. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 23 TC 2 Z9 2 U1 2 U2 2 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2016 VL 99 SI 1 BP 215 EP 221 PG 7 WC Entomology SC Entomology GA DU5JZ UT WOS:000382249300025 ER PT J AU Makarem, N Nicholson, JM Bandera, EV McKeown, NM Parekh, N AF Makarem, Nour Nicholson, Joseph M. Bandera, Elisa V. McKeown, Nicola M. Parekh, Niyati TI Consumption of whole grains and cereal fiber in relation to cancer risk: a systematic review of longitudinal studies SO NUTRITION REVIEWS LA English DT Review DE cancer risk; cereal fiber; longitudinal studies; systematic review; whole grains ID POSTMENOPAUSAL BREAST-CANCER; IOWA WOMENS HEALTH; DIETARY PHYTOESTROGEN INTAKE; NIH-AARP DIET; COLORECTAL-CANCER; UNITED-STATES; PROSPECTIVE COHORT; COLON-CANCER; ENDOMETRIAL CANCER; NATIONAL-HEALTH AB Context: Evidence from previous reviews is supportive of the hypothesis that whole grains may protect against various cancers. However, the reviews did not report risk estimates for both whole grains and cereal fiber and only case-control studies were evaluated. It is unclear whether longitudinal studies support this conclusion. Objective: To evaluate associations between whole grains and cereal fiber in relation to risk of lifestyle-related cancers data from longitudinal studies was evaluated. Data Sources: The following 3 databases were systematically searched: PubMed, EMBASE, and Cochrane CENTRAL. Study Selection: A total of 43 longitudinal studies conducted in Europe and North America that reported multivariable-adjusted risk estimates for whole grains (n = 14), cereal fiber (n = 23), or both (n = 6) in relation to lifestyle-related cancers were included. Data Extraction: Information on study location, cohort name, follow-up duration, sample characteristics, dietary assessment method, risk estimates, and confounders was extracted. Data Synthesis: Of 20 studies examining whole grains and cancer, 6 studies reported a statistically significant 6%-47% reduction in risk, but 14 studies showed no association. Of 29 studies examining cereal fiber intake in relation to cancer, 8 showed a statistically significant 6%-49% reduction in risk, whereas 21 studies reported no association. Conclusions: This systematic review concludes that most studies were suggestive of a null association. Whole grains and cereal fiber may protect against gastrointestinal cancers, but these findings require confirmation in additional studies. C1 [Makarem, Nour] NYU, Dept Nutr Food Studies & Publ Hlth, New York, NY USA. [Nicholson, Joseph M.] NYU, Sch Med, Dept Med Lib, New York, NY USA. [Bandera, Elisa V.] Rutgers State Univ, Rutgers Sch Publ Hlth, New Brunswick, NJ USA. [Bandera, Elisa V.] Rutgers Canc Inst New Jersey, New Brunswick, NJ USA. [McKeown, Nicola M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [McKeown, Nicola M.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Parekh, Niyati] NYU, Coll Global Publ Hlth, Dept Nutr Food Studies & Publ Hlth, New York, NY USA. [Parekh, Niyati] NYU, Dept Populat Hlth, New York, NY USA. RP Parekh, N (reprint author), NYU, Coll Global Publ Hlth, 411 Lafayette St,Room 542, New York, NY 10003 USA. EM niyati.parekh@nyu.edu OI Nicholson, Joey/0000-0001-8658-5879; parekh , niyati /0000-0002-1334-0528 FU American Cancer Society [RSG-12-005-01-CNE] FX This research was supported by the American Cancer Society Research Scholar Grant (#RSG-12-005-01-CNE) awarded to N.P. The funder did not play any role in the design, execution, or approval of this review. NR 74 TC 2 Z9 2 U1 8 U2 10 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0029-6643 EI 1753-4887 J9 NUTR REV JI Nutr. Rev. PD JUN PY 2016 VL 74 IS 6 BP 353 EP 373 DI 10.1093/nutrit/nuw003 PG 21 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DV9RK UT WOS:000383278600001 PM 27257283 ER PT J AU Ainsworth, EA AF Ainsworth, Elizabeth A. TI The importance of intraspecific variation in tree responses to elevated [CO2]: breeding and management of future forests SO TREE PHYSIOLOGY LA English DT Article ID ASSISTED GENE FLOW; ATMOSPHERIC CO2; CLIMATE-CHANGE; GROWTH-RESPONSES; CARBON-DIOXIDE; PRODUCTIVITY; ADAPTATION; ENRICHMENT; DYNAMICS; METAANALYSIS C1 [Ainsworth, Elizabeth A.] USDA ARS, Global Change & Photosynth Res Unit, 1201 W Gregory Dr, Urbana, IL 61801 USA. RP Ainsworth, EA (reprint author), USDA ARS, Global Change & Photosynth Res Unit, 1201 W Gregory Dr, Urbana, IL 61801 USA. EM lisa.ainsworth@ars.usda.gov NR 27 TC 0 Z9 0 U1 8 U2 9 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0829-318X EI 1758-4469 J9 TREE PHYSIOL JI Tree Physiol. PD JUN PY 2016 VL 36 IS 6 BP 679 EP 681 DI 10.1093/treephys/tpw039 PG 3 WC Forestry SC Forestry GA DT2AQ UT WOS:000381283600001 PM 27188497 ER PT J AU Atwood, TC Marcot, BG Douglas, DC Amstrup, SC Rode, KD Durner, GM Bromaghin, JF AF Atwood, Todd C. Marcot, Bruce G. Douglas, David C. Amstrup, Steven C. Rode, Karyn D. Durner, George M. Bromaghin, Jeffrey F. TI Forecasting the relative influence of environmental and anthropogenic stressors on polar bears SO ECOSPHERE LA English DT Article DE Arctic; Bayesian network; climate change; conservation; greenhouse gas emissions; influence analysis; mitigation; stressor evaluation; Ursus maritimus ID WESTERN HUDSON-BAY; ARCTIC MARINE MAMMALS; SOUTHERN BEAUFORT SEA; ICE-FREE PERIOD; CLIMATE-CHANGE; URSUS-MARITIMUS; POPULATION ECOLOGY; FOOD AVAILABILITY; BAYESIAN NETWORK; BODY CONDITION AB Effective conservation planning requires understanding and ranking threats to wildlife populations. We developed a Bayesian network model to evaluate the relative influence of environmental and anthropogenic stressors, and their mitigation, on the persistence of polar bears (Ursus maritimus). Overall sea ice conditions, affected by rising global temperatures, were the most influential determinant of population outcomes. Accordingly, unabated rise in atmospheric greenhouse gas (GHG) concentrations was the dominant influence leading to worsened population outcomes, with polar bears in three of four ecoregions reaching a dominant probability of decreased or greatly decreased by the latter part of this century. Stabilization of atmospheric GHG concentrations by mid-century delayed the greatly reduced state by approximate to 25 yr in two ecoregions. Prompt and aggressive mitigation of emissions reduced the probability of any regional population becoming greatly reduced by up to 25%. Marine prey availability, linked closely to sea ice trend, had slightly less influence on outcome state than sea ice availability itself. Reduced mortality from hunting and defense of life and property interactions resulted in modest declines in the probability of a decreased or greatly decreased population outcome. Minimizing other stressors such as trans-Arctic shipping, oil and gas exploration, and contaminants had a negligible effect on polar bear outcomes, although the model was not well-informed with respect to the potential influence of these stressors. Adverse consequences of loss of sea ice habitat became more pronounced as the summer ice-free period lengthened beyond four months, which could occur in most of the Arctic basin after mid-century if GHG emissions are not promptly reduced. Long-term conservation of polar bears would be best supported by holding global mean temperature to <= 2 degrees C above preindustrial levels. Until further sea ice loss is stopped, management of other stressors may serve to slow the transition of populations to progressively worsened outcomes, and improve the prospects for their long-term persistence. C1 [Atwood, Todd C.; Rode, Karyn D.; Durner, George M.; Bromaghin, Jeffrey F.] US Geol Survey, Alaska Sci Ctr, Anchorage, AK 99508 USA. [Marcot, Bruce G.] US Forest Serv, Pacific Northwest Res Stn, USDA, Portland, OR 97208 USA. [Douglas, David C.] US Geol Survey, Alaska Sci Ctr, Juneau, AK 99801 USA. [Amstrup, Steven C.] Polar Bears Int, Bozeman, MT 59772 USA. RP Atwood, TC (reprint author), US Geol Survey, Alaska Sci Ctr, Anchorage, AK 99508 USA. EM tatwood@usgs.gov RI Bromaghin, Jeffrey/B-5058-2009; OI Bromaghin, Jeffrey/0000-0002-7209-9500; Rode, Karyn/0000-0002-3328-8202 FU U.S. Geological Survey Ecosystems Mission Area; U.S. Fish and Wildlife Service, Region 7, Marine Mammals Management Office FX This analysis is part of the U.S. Geological Survey Changing Arctic Ecosystems Initiative, supported by the U.S. Geological Survey Ecosystems Mission Area, with additional in-kind contributions from the U.S. Forest Service, Pacific Northwest Research Station. Additional support was provided by the U.S. Fish and Wildlife Service, Region 7, Marine Mammals Management Office. We acknowledge the World Climate Research Programme's Working Group on Coupled Modelling, which is responsible for CMIP, and we thank the climate modeling groups (listed in Appendix S1: Table S1) for producing and making available their model output. We thank K. Laidre for providing expertise on polar bear ecology and for valuable insight throughout the course of this study. We thank members of the U.S. Polar Bear Recovery Team, Science and Traditional Ecological Knowledge Working Group for providing feedback on model influence diagrams and structure. We are grateful for the helpful comments and suggestions from T. DeBruyn, J. Wilder, M. Runge, and D. Pierce-Williams at various stages in the development of this work. K. Oakley, S. Ban, G. Wilhere, and A. Derocher provided comments on earlier versions of this manuscript. Use of trade names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 102 TC 2 Z9 2 U1 79 U2 103 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD JUN PY 2016 VL 7 IS 6 AR e01370 DI 10.1002/ecs2.1370 PG 22 WC Ecology SC Environmental Sciences & Ecology GA DU4CD UT WOS:000382158500001 ER PT J AU Friesen, K Berkebile, D Wienhold, B Durso, L Zhu, J Taylor, DB AF Friesen, Kristina Berkebile, Dennis Wienhold, Brian Durso, Lisa Zhu, Jerry Taylor, David B. TI Environmental Parameters Associated With Stable Fly (Diptera: Muscidae) Development at Hay Feeding Sites SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Stomoxys calcitrans; larval substrate; pest management; coliform ID HOUSE-FLIES DIPTERA; STOMOXYS-CALCITRANS DIPTERA; EASTERN NEBRASKA; ESCHERICHIA-COLI; CATTLE; LARVAE; TEMPERATURE; POPULATIONS; ABUNDANCE; BACTERIA AB Substrates composed of hay residues, dung, and urine accumulate around winter hay feeding sites in cattle pastures, providing developmental habitats for stable flies. The objective of this study was to relate physiochemical and microbial properties of these substrates to the presence or absence of stable fly larvae. Properties included pH, temperature, moisture, ammonium concentration, electrical conductivity, and numbers of coliform, fecal coliform, Escherichia coli, and Enterococcus bacteria. Each physiochemical sample was classified as a function of belonging to one of the three 2-m concentric zones radiating from the feeder as well as presence or absence of larvae. In total, 538 samples were collected from 13 sites during 2005-2011. Stable fly larvae were most likely to be found in moist, slightly alkaline substrates with high levels of ammonium and low temperature. The probability of larvae being present in a sample was the highest when the moisture content was 347% relative to dry weight and the average pH was 8.4. Larvae were recovered within all zones, with a nonsignificant, but slightly higher, percentage of samples containing larvae taken 2-4 m from the center. All methods used to enumerate bacteria, except total coliform, indicated decreasing concentrations in hay bale residue throughout the summer. In addition to the environmental parameters, cumulative degree day 10 degrees C had a significant effect on the probability of observing stable fly larvae in a sample, indicating that unidentified seasonal effects also influenced immature stable fly populations. C1 [Friesen, Kristina; Berkebile, Dennis; Wienhold, Brian; Durso, Lisa; Zhu, Jerry; Taylor, David B.] ARS, USDA, Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. RP Friesen, K (reprint author), ARS, USDA, Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. EM Kristina.friesen@ars.usda.gov; Dennis.berkebile@ars.usda.gov; Brian.wienhold@ars.usda.gov; Lisa.durso@ars.usda.gov; Jerry.zhu@ars.usda.gov; Dave.taylor@ars.usda.gov NR 38 TC 1 Z9 1 U1 3 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2016 VL 45 IS 3 BP 570 EP 576 DI 10.1093/ee/nvw014 PG 7 WC Entomology SC Entomology GA DU0QY UT WOS:000381910800004 ER PT J AU Pureswaran, DS Hofstetter, RW Sullivan, BT Potter, KA AF Pureswaran, Deepa S. Hofstetter, Richard W. Sullivan, Brian T. Potter, Kristen A. TI The Role of Multimodal Signals in Species Recognition Between Tree-Killing Bark Beetles in a Narrow Sympatric Zone SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE acoustic signal; character displacement; Dendroctonus; multispecies aggregation; pheromone ID SOUTHERN PINE-BEETLE; DENDROCTONUS-FRONTALIS COLEOPTERA; SOUND PRODUCTION; ENDO-BREVICOMIN; AGGREGATION PHEROMONE; EXO-BREVICOMIN; SCOLYTIDAE; CURCULIONIDAE; STRIDULATION; EVOLUTION AB When related species coexist, selection pressure should favor evolution of species recognition mechanisms to prevent interspecific pairing and wasteful reproductive encounters. We investigated the potential role of pheromone and acoustic signals in species recognition between two species of tree-killing bark beetles, the southern pine beetle, Dendroctonus frontalis Zimmermann, and the western pine beetle, Dendroctonus brevicomis LeConte, in a narrow zone of sympatry, using reciprocal pairing experiments. Given the choice of adjacent con-or heterospecific female gallery entrance in a log, at least 85% of walking males chose the entrance of the conspecific, and half the males that initially entered heterospecific galleries re-emerged and entered the conspecific gallery within 15 min. Waveform analysis of female acoustic "chirps" indicated interspecific differences in chirp timing. Males may use information from female acoustic signals to decide whether to enter or remain in the gallery. Individuals in forced heterospecific pairings (produced by confinement of a heterospecific male within the female entrance) did not differ in pheromone production from individuals of conspecific pairs. However, due to the absence of the right species of male, galleries with heterospecific pairs released an abnormal pheromone blend that lacked at least one key component of the aggregation pheromone of either species. The complete aggregation pheromone (i.e., the pheromone blend from entrances with pairs) does not appear to deter interspecific encounters or confer premating reproductive isolation per se; however, it may confer selective pressure for the maintenance of other reproductive isolation mechanisms. C1 [Pureswaran, Deepa S.] Laurentian Forestry Ctr, Canadian Forest Serv, 1055,Rue PEPS, Quebec City, PQ G1V 4C7, Canada. [Hofstetter, Richard W.] No Arizona Univ, Sch Forestry, Box 15018, Flagstaff, AZ 86011 USA. [Sullivan, Brian T.] USDA Forest Serv, Southern Res Stn, Pineville, LA 71360 USA. [Potter, Kristen A.] No Arizona Univ, Landscape Conservat Initiat, Box 5767, Flagstaff, AZ 86011 USA. RP Hofstetter, RW (reprint author), No Arizona Univ, Sch Forestry, Box 15018, Flagstaff, AZ 86011 USA. EM deepa.pureswaran@canada.ca; rich.hofstetter@nau.edu; briansullivan@fs.fed.us; kristen.potter@nau.edu FU Canadian Forest Service OM funds [08-JV-11221633-250]; Northern Arizona University School of Forestry; Forest Service FX We thank Reagan McGuire, Seth Davis, and Eli Jenson for their assistance in collecting insects and phloem. We thank the Rocky Mountain Research Station in Flagstaff, AZ, for use of their facilities, and P. Duval and S. Bourassa for making Figure 1. This project was funded by Canadian Forest Service O&M funds to D.P. and U.S.D.A. Cooperative Agreement 08-JV-11221633-250 to R.H., and supported by the Northern Arizona University School of Forestry and U.S.D.A. Forest Service. NR 50 TC 1 Z9 1 U1 1 U2 1 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2016 VL 45 IS 3 BP 582 EP 591 DI 10.1093/ee/nvw022 PG 10 WC Entomology SC Entomology GA DU0QY UT WOS:000381910800006 ER PT J AU Johnson, AJ Kendra, PE Skelton, J Hulcr, J AF Johnson, Andrew J. Kendra, Paul E. Skelton, James Hulcr, Jiri TI Species Diversity, Phenology, and Temporal Flight Patterns of Hypothenemus Pygmy Borers (Coleoptera: Curculionidae: Scolytinae) in South Florida SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE bark beetle; phenology; diversity; diurnal; circadian ID XYLEBORUS-GLABRATUS; AMBROSIA BEETLES; OIL LURES AB Hypothenemus are some of the most common and diverse bark beetles in natural as well as urban habitats, particularly in tropical and subtropical regions. Despite their ecological success and ubiquitous presence, very little is known about the habits of this genus. This study aimed to understand species diversity and daily and seasonal trends in host-seeking flight patterns of Hypothenemus in a suburban environment by systematic collections with ethanol baiting over a 15-mo period in South Florida. A total of 481 specimens were collected and identified as eight species, most of them nonnative. Hypothenemus formed the overwhelming majority of bark beetles (Scolytinae) collected, confirming the dominance of the genus in urban environments. Hypothenemus brunneus (Hopkins) and Hypothenemus seriatus (Eichhoff) were most abundant, comprising 74% of the capture. Rarefaction showed that the sample was sufficient to characterize the local diversity and composition. The seasonal pattern in Hypothenemus capture was positively correlated to day-time temperature, not to season as in most temperate Scolytinae. Another significant observation in the community dynamics was the synchronized occurrence of two common species (H. birmanus and H. javanus), unrelated to season. Hypothenemus were predominantly diurnal with a broad flight window. Females flew as early as 11: 00 hours (EDST), with peak flight occurring at 15: 00 hours, significantly earlier than flight patterns of most other Scolytinae. Surprisingly, male Hypothenemus were frequently collected, despite their lack of functional wings. Several potential explanations are discussed. This is the first study into the ecology of an entire community of the twig-feeding Hypothenemus. C1 [Johnson, Andrew J.; Skelton, James; Hulcr, Jiri] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [Kendra, Paul E.] USDA ARS, Subtrop Hort Res Stn, Miami, FL 33158 USA. [Hulcr, Jiri] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. RP Kendra, PE (reprint author), USDA ARS, Subtrop Hort Res Stn, Miami, FL 33158 USA. EM andrewjavanjohnson@gmail.com; paul.kendra@ars.usda.gov; skelto3@gmail.com; hulcr@ufl.edu FU United States Department of Agriculture (USDA) Forest Service (FS)-SRS Coop agreement [14-CA-11330130-032]; USDA-FS-FHP Coop agreement [12-CA-11420004-042]; USDA Farm Bill agreement [14-8130-0377-CA]; National Science Foundation [DEB 1256968]; USDA-ARS Appropriated Funds FX A.J.J. and J.H. were funded by the United States Department of Agriculture (USDA) Forest Service (FS)-SRS Coop agreement 14-CA-11330130-032, USDA-FS-FHP Coop agreement 12-CA-11420004-042, USDA Farm Bill agreement 14-8130-0377-CA, and the National Science Foundation DEB 1256968. P. K. was funded by USDA-ARS Appropriated Funds (Mitigation of the Invasive Pest Threat from the American Tropics and Subtropics). Mention of a proprietary product does not constitute an endorsement by the USDA-ARS. NR 23 TC 0 Z9 0 U1 6 U2 6 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2016 VL 45 IS 3 BP 627 EP 632 DI 10.1093/ee/nvw039 PG 6 WC Entomology SC Entomology GA DU0QY UT WOS:000381910800010 ER PT J AU Mathews, CR Brown, MW Wackers, FL AF Mathews, Clarissa R. Brown, Mark W. Wackers, Felix L. TI Comparison of Peach Cultivars for Provision of Extrafloral Nectar Resources to Harmonia axyridis (Coleoptera: Coccinellidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE conservation biological control; extrafloral nectar; aphidophagy; nonprey food; Asian lady beetle ID CONSERVATION BIOLOGICAL-CONTROL; GRAPHOLITA-MOLESTA LEPIDOPTERA; ALTERNATIVE FOOD; APHIS-SPIRAECOLA; NATURAL ENEMIES; WEST-VIRGINIA; SPIREA APHID; APPLE; TORTRICIDAE; ROSACEAE AB Incorporating nonprey sugar resources into apple orchards is a potential means of enhancing biological control services, but little is known about the impacts of extrafloral nectars on aphidophagous coccinellids. We explored peach Prunus persica (L.) Batsch extrafloral nectar as a supplemental resource for Harmonia axyridis (Pallas), a key aphid predator in the mid-Atlantic United States. Extrafloral nectar quantity, temporal production, and carbohydrate profile were assessed for four peach cultivars in orchard and greenhouse culture. Seasonal densities of H. axyridis visiting extrafloral nectaries were estimated, and the propensity of beetles to feed upon extrafloral nectar was compared by cultivar in the laboratory. We also compared survival of newly eclosed adult pairs that were starved or fed aphids with or without extrafloral nectar. Peach extrafloral nectar contained six carbohydrates, with sucrose dominant for all cultivars, but extrafloral nectar production varied significantly by cultivar and collection date, with 'Lovell' yielding higher average seasonal volume than the other cultivars. Harmonia axyridis continuously foraged on peach trees lacking prey, and beetle abundance was positively correlated with the number of leaves actively producing extrafloral nectar. In laboratory assays, newly emerged adult beetles preferentially selected and consumed extrafloral nectar of Lovell peach shoots. Furthermore, when prey were initially unavailable to adult H. axyridis, the beetles were sustained by extrafloral nectar and experienced longer survival compared with beetles without the supplemental resource. Collectively, these results suggest that peach extrafloral nectar is a beneficial resource that could potentially sustain H. axyridis in orchards when prey are scarce. C1 [Mathews, Clarissa R.] Shepherd Univ, Inst Environm & Phys Sci, Shepherdstown, WV 25443 USA. [Brown, Mark W.] 28 Church Lane, Marshfield, ME 04654 USA. [Brown, Mark W.] USDA ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25414 USA. [Wackers, Felix L.] Biobest, Westerlo, Belgium. RP Mathews, CR (reprint author), Shepherd Univ, Inst Environm & Phys Sci, Shepherdstown, WV 25443 USA. EM CMathews@shepherd.edu; mwb_ifp@yahoo.com FU National Research Initiative of the United States Department of Agriculture Cooperative State Research, Education and Extension Service Grant [2003-35316-13773] FX This project was supported by the National Research Initiative of the United States Department of Agriculture Cooperative State Research, Education and Extension Service Grant 2003-35316-13773. NR 34 TC 0 Z9 0 U1 6 U2 7 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2016 VL 45 IS 3 BP 649 EP 657 DI 10.1093/ee/nvw035 PG 9 WC Entomology SC Entomology GA DU0QY UT WOS:000381910800013 ER PT J AU Minteer, CR Kring, TJ Wiedenmann, RN AF Minteer, C. R. Kring, T. J. Wiedenmann, R. N. TI Larinus minutus (Coleoptera: Curculionidae) and Urophora quadrifasciata (Diptera: Tephritidae), Evidence for Interaction and Impact on Spotted Knapweed in Arkansas SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE spotted knapweed; Centaurea stoebe ssp micranthos; Larinus minutus; Urophora quadrifasciata; interaction ID BIOLOGICAL-CONTROL AGENTS; INTERSPECIFIC INTERACTIONS; PHYTOPHAGOUS INSECTS; DIFFUSE; COMPETITION; AFFINIS; GALL; ESTABLISHMENT; CENTAUREA AB Interactions among seed-feeding insects are well noted in the literature, with many of these interactions being asymmetrical and causing a disadvantage for one of the species involved. While often effective, the use of the cumulative stress approach to biological control (where multiple natural enemy species are released) may increase the risk of interaction, which may lead to less effective biological control programs. Spotted knapweed (Centaurea stoebe ssp. micranthos (Gugler) Hayek) is an invasive plant that causes damage in rangelands and pastures. In Arkansas, two biological control agents for spotted knapweed, Larinus minutus Gyllenhal and Urophora quadrifasciata Meigen, are established. Both species attack the capitula of spotted and diffuse knapweeds. There is potential for interactions between these species, and this could affect the efficacy of the biological control program. To evaluate these potential interactions, we collected spotted knapweed capitula weekly from three sites in Arkansas from 2010 to 2012. Capitula were monitored for emergence of L. minutus and U. quadrifasciata, and the effect on seed reduction was analyzed. Over all three years, L. minutus and U. quadrifasciata were present in 35.8% (492) and 22.8% (313) of the capitula, respectively. We determined that the occurrences of L. minutus and U. quadrifasciata were not independent of each other, and that L. minutus is more effective at reducing spotted knapweed seed production than U. quadrifasciata within individual capitula. Seed production in capitula with both natural enemies present was no different from capitula with L. minutus. C1 [Minteer, C. R.] USDA ARS, Invas Plant Res Lab, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. [Kring, T. J.; Wiedenmann, R. N.] Univ Arkansas, Dept Entomol, 319 AGRI, Fayetteville, AR 72701 USA. RP Minteer, CR (reprint author), USDA ARS, Invas Plant Res Lab, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. EM minteer7@gmail.com; tkring@uark.edu; rwieden@uark.edu OI Kring, Timothy/0000-0002-4920-8553 FU United States Department of Agriculture (USDA)- Forest Service, Forest Health Technology Enterprise Team; USDA-Animal and Plant Health Inspection Service -Plant Protection and Quarantine; Arkansas Agriculture Experiment Station FX We thank June Shen for assistance in the lab and the field, Jessica Hartshorn for the help with R, and Johnnie Gentry, Fred Stephen, Raghu Sathyamurthy, and Jason Tullis for reviewing the manuscript. Funding was provided by the United States Department of Agriculture (USDA)- Forest Service, Forest Health Technology Enterprise Team; USDA-Animal and Plant Health Inspection Service -Plant Protection and Quarantine; and the Arkansas Agriculture Experiment Station. NR 30 TC 0 Z9 0 U1 6 U2 6 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2016 VL 45 IS 3 BP 658 EP 662 DI 10.1093/ee/nvw011 PG 5 WC Entomology SC Entomology GA DU0QY UT WOS:000381910800014 ER PT J AU Acebes-Doria, AL Leskey, TC Bergh, JC AF Acebes-Doria, Angelita L. Leskey, Tracy C. Bergh, J. Christopher TI Host Plant Effects on Halyomorpha halys (Hemiptera: Pentatomidae) Nymphal Development and Survivorship SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE host plant suitability; brown marmorated stink bug; mixed diet; polyphagy; nymph ID MARMORATED STINK BUG; NEZARA-VIRIDULA; HETEROPTERA-PENTATOMIDAE; AILANTHUS-ALTISSIMA; STAL HETEROPTERA; WILD HOSTS; TREE FRUIT; BEHAVIOR; DIET; HERBIVORE AB Halyomorpha halys (Stal) (Hemiptera: Pentatomidae) is a highly polyphagous invasive species and an important pest of orchard crops in the United States. In the Mid-Atlantic region, wild hosts of H. halys are common in woodlands that often border orchards, and H. halys movement from them into orchards poses ongoing management issues. To improve our understanding of host plant effects on H. halys populations at the orchard-woodland interface, nymphal survivorship, developmental duration, and adult fitness (size and fresh weight) on apple (Malus domestica Borkh.), peach (Prunus persica (L.) Batsch), Tree of Heaven (Ailanthus altissima (Mill.) Swingle), and northern catalpa (Catalpa speciosa (Warder)) were examined in laboratory studies. Specifically, we investigated nymphal performance on the foliage and fruiting structures of those hosts and on single-versus mixed-host diets, as well as the effects of host phenology on their suitability. Nymphal performance was poor on a diet of foliage alone, regardless of host. When fruiting structures were combined with foliage, peach was highly suitable for nymphal development and survivorship, whereas apple, Tree of Heaven, and catalpa were less so, although nymphal survival on Tree of Heaven was much greater later in the season than earlier. Mixed-host diets yielded increased nymphal survivorship and decreased developmental duration compared with diets of suboptimal single hosts. Adult size and weight were generally greater when they developed from nymphs reared on mixed diets. The implications of our results to the dispersal behavior, establishment, and management of H. halys are discussed. C1 [Acebes-Doria, Angelita L.; Bergh, J. Christopher] Virginia Tech, Alson H Smith Jr Agr Res & Extens Ctr, Winchester, VA 22602 USA. [Leskey, Tracy C.] USDA ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. RP Acebes-Doria, AL (reprint author), Virginia Tech, Alson H Smith Jr Agr Res & Extens Ctr, Winchester, VA 22602 USA. EM aacebes@vt.edu; tracy.leskey@ars.usda.gov; cbergh@vt.edu FU National Institute of Food and Agriculture, U.S. Department of Agriculture [2011-51181-30937] FX We are greatly thankful for Dr. Shimat Joseph, Sean Gresham, Jean Engelman, Eliezer Doria, and the student assistants for their excellent technical assistance. This material is based on work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award number #2011-51181-30937. NR 41 TC 1 Z9 1 U1 6 U2 6 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2016 VL 45 IS 3 BP 663 EP 670 DI 10.1093/ee/nvw018 PG 8 WC Entomology SC Entomology GA DU0QY UT WOS:000381910800015 ER PT J AU Light, DM AF Light, Douglas M. TI Control and Monitoring of Codling Moth (Lepidoptera: Tortricidae) in Walnut Orchards Treated With Novel High-Load, Low-Density "Meso" Dispensers of Sex Pheromone and Pear Ester SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE walnut; Cydia pomonella; Amyelois transitella; mating disruption; monitoring ID PLUS ACETIC-ACID; CYDIA-POMONELLA; MATING DISRUPTION; CODLEMONE; KAIROMONE; APPLE; LURES; (E)-4,8-DIMETHYL-1,3,7-NONATRIENE; EFFICACY; BLENDS AB Low-density per ha "meso" dispensers loaded with pear ester, ethyl (E,Z)-2,4-decadienoate, kairomone and codlemone, (E, E)-8,10-dodecadien-1-ol, pheromone of codling moth, Cydia pomonella (L)., were evaluated versus meso dispensers loaded with pheromone alone for mating disruption control in walnut orchards receiving no insecticide sprays. Meso dispensers loaded with codlemone alone (Ph meso) were applied at 50 ha(-1) and compared with mesos combining codlemone and pear ester (Ph+PE meso) at 25 and 50 ha(-1). Various lures containing pear ester (PE), Ph-PE combo, and an experimental codlemone plus (E)-4,8-dimethyl-1,3,7-nonatriene lure were tested alone and with acetic acid (AA) lures for moth capture efficacy. Male moth capture in pheromone traps was significantly reduced by 88% in Ph meso plots and 96% in Ph+PE meso plots versus control plots. Moth capture in Ph-PE combo traps was significantly reduced for both sexes in all meso plots. Harvest damage by both the codling moth and the secondary pest, navel orangeworm, Amyelois transitella (Walker), was significantly lower in all meso treatment plots compared with damage in control plots. Nut injury level with the Ph+PE meso treatment (50 ha(-1)) was significantly lower than in Ph meso plots for both codling moth and combined pest injury. Regression analysis suggested that nut infestation levels by navel orangeworm were influenced by codling moth levels. In all meso plots, the most effective lures attracting both codling moth sexes were PE & AA or Ph-PE combo & AA. Demonstrated disruption and control efficacy of these pheromone plus PE-meso dispensers applied at low densities supports development of the meso dispenser tactic for practical pest management use in walnut orchards with inherent low planting densities. C1 [Light, Douglas M.] ARS, USDA, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. RP Light, DM (reprint author), ARS, USDA, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM doug.light@ars.usda.gov NR 42 TC 1 Z9 1 U1 7 U2 7 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2016 VL 45 IS 3 BP 700 EP 707 DI 10.1093/ee/nvw017 PG 8 WC Entomology SC Entomology GA DU0QY UT WOS:000381910800020 ER PT J AU Hoban, J Duan, JJ Hough-Goldstein, J AF Hoban, J. Duan, J. J. Hough-Goldstein, J. TI Effects of Temperature and Photoperiod on the Reproductive Biology and Diapause of Oobius agrili (Hymenoptera: Encyrtidae), an Egg Parasitoid of Emerald Ash Borer (Coleoptera: Buprestidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE biological control; invasive species; Agrilus planipennis; natural enemy; reproduction ID NORTH-AMERICA; ESTABLISHMENT; EULOPHIDAE; HISTORY; CHINA AB Oobius agrili Zhang and Huang (Hymenoptera: Encyrtidae) is a solitary egg parasitoid of the invasive emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), and has been introduced to the United States for classical biological control. We characterized the weekly survivorship, fecundity, and diapause patterns of both diapaused and nondiapaused populations of O. agrili under four different temperature-photophase combinations: 30 degrees C (warm) and 20 degrees C (cold) temperatures with both long-day (16 h) and short-day (8 h) photophase. Results of this study showed that regardless of the length of photophase, parental parasitoids of both diapaused and nondiapaused O. agrili survived significantly longer at 20 degrees C than at 30 degrees C. Both populations also laid their eggs faster at 30 degrees C compared with those at 20 degrees C. Higher proportions of the progeny produced by both populations of O. agrili were induced into diapause by short-day (8 h) photophase, regardless of rearing temperature. In addition, the diapaused parasitoids in the short-day photophase treatment at both warm and cold temperatures produced increasing proportions of diapaused progeny over time, whereas no significant differences were observed in the proportions of diapaused progeny by the nondiapaused parasitoids over different sampling times. These findings suggest that O. agrili should be continuously reared under warm temperature and long-day photoperiod (to avoid diapause for increased reproduction). In addition, we recommend that diapaused adults be used for field releases in early summer when temperatures are still relatively low (similar to 20 degrees C) and host eggs are available so that they can produce multiple generations prior to overwintering. C1 [Hoban, J.; Duan, J. J.] ARS, USDA, Beneficial Insect Intro Unit, Newark, DE 19711 USA. [Hoban, J.; Hough-Goldstein, J.] Univ Delaware, Dept Entomol & Wildlife Ecol, Newark, DE 19716 USA. RP Hoban, J (reprint author), ARS, USDA, Beneficial Insect Intro Unit, Newark, DE 19711 USA.; Hoban, J (reprint author), Univ Delaware, Dept Entomol & Wildlife Ecol, Newark, DE 19716 USA. EM jhoban@udel.edu; jian.duan@ars.usda.gov; jhough@udel.edu NR 20 TC 1 Z9 1 U1 5 U2 5 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2016 VL 45 IS 3 BP 726 EP 731 DI 10.1093/ee/nvw034 PG 6 WC Entomology SC Entomology GA DU0QY UT WOS:000381910800023 ER PT J AU Chasen, EM Steffan, SA AF Chasen, E. M. Steffan, S. A. TI Temperature-Mediated Growth Thresholds of Acrobasis vaccinii (Lepidoptera: Pyralidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE cranberry fruitworm; degree-day model; integrated pest management; cranberry; Vaccinium macrocarpon ID CRANBERRY FRUITWORM; PEST-MANAGEMENT; MODELS; TORTRICIDAE; COLEOPTERA; CONSTANT AB Degree-day models link ambient temperature to the development of insects, making such models valuable tools in integrated pest management. These models increase management efficacy by quantifying and predicting pest phenology. In Wisconsin, the top insect pest of cranberry production is the cranberry fruitworm, Acrobasis vaccinii Riley (Lepidoptera: Pyralidae). Control of this species is often complicated by the fact that the larvae feed entirely within the fruit. Timing of control tactics, therefore, is critical and generally targets the adult and egg stages. However, the commencement of oviposition and egg hatch are extremely difficult to track empirically, forcing pest management strategies to rely on proxy events that are more apparent but less informative as indicators of cranberry fruitworm egg presence. This research provides the upper and lower temperature-mediated growth thresholds of this pest, which represents the first steps toward the creation of a degree-day model. Using field-collected A. vaccinii, we reared the larvae within cranberry fruit and monitored larval growth at nine different constant temperatures. We determined the average growth rate at each temperature and modeled growth rates as a function of temperature. We then calculated the precise upper and lower developmental temperature thresholds of this species. Future work will be able to use these thresholds to generate degree-day accumulations that correspond to phenological events in the field, providing a powerful predictive tool for pest management in cranberry production. C1 [Chasen, E. M.; Steffan, S. A.] USDA ARS, Vegetable Crops Res Unit, 1630 Linden Dr, Madison, WI 53706 USA. [Chasen, E. M.; Steffan, S. A.] Univ Wisconsin, Dept Entomol, 1630 Linden Dr, Madison, WI 53706 USA. RP Steffan, SA (reprint author), USDA ARS, Vegetable Crops Res Unit, 1630 Linden Dr, Madison, WI 53706 USA.; Steffan, SA (reprint author), Univ Wisconsin, Dept Entomol, 1630 Linden Dr, Madison, WI 53706 USA. EM emchasen@gmail.com; shawn.steffan@ars.usda.gov FU USDA-ARS [3655-21220-001]; Wisconsin Cranberry Board; Cranberry Institute FX Much gratitude is extended to our Wisconsin cranberry grower-collaborators for allowing us to collect materials for these experiments at their marshes. Thanks also to Lady Bug IPM and Ocean Spray Cranberries for their assistance in this project. Funding was provided by the USDA-ARS (appropriated funds 3655-21220-001), the Wisconsin Cranberry Board, and the Cranberry Institute. NR 30 TC 0 Z9 0 U1 4 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2016 VL 45 IS 3 BP 732 EP 736 DI 10.1093/ee/nvw053 PG 5 WC Entomology SC Entomology GA DU0QY UT WOS:000381910800024 ER PT J AU Dhakal, S Chao, KL Schmidt, W Qin, JW Kim, M Chan, DE AF Dhakal, Sagar Chao, Kuanglin Schmidt, Walter Qin, Jianwei Kim, Moon Chan, Diane TI Evaluation of Turmeric Powder Adulterated with Metanil Yellow Using FT-Raman and FT-IR Spectroscopy SO FOODS LA English DT Article DE FT-Raman; FT-IR; turmeric powder; metanil yellow; quantitative analysis ID HEPATIC PRENEOPLASTIC LESIONS; CURCUMA-LONGA L.; VIRGIN OLIVE OIL; N-NITROSODIETHYLAMINE; MALACHITE GREEN; LIQUID-CHROMATOGRAPHY; ANTIOXIDANT ACTIVITY; MASS-SPECTROMETRY; IMAGING-SYSTEM; IN-VITRO AB Turmeric powder (Curcuma longa L.) is valued both for its medicinal properties and for its popular culinary use, such as being a component in curry powder. Due to its high demand in international trade, turmeric powder has been subject to economically driven, hazardous chemical adulteration. This study utilized Fourier Transform-Raman (FT-Raman) and Fourier Transform-Infra Red (FT-IR) spectroscopy as separate but complementary methods for detecting metanil yellow adulteration of turmeric powder. Sample mixtures of turmeric powder and metanil yellow were prepared at concentrations of 30%, 25%, 20%, 15%, 10%, 5%, 1%, and 0.01% (w/w). FT-Raman and FT-IR spectra were acquired for these mixture samples as well as for pure samples of turmeric powder and metanil yellow. Spectral analysis showed that the FT-IR method in this study could detect the metanil yellow at the 5% concentration, while the FT-Raman method appeared to be more sensitive and could detect the metanil yellow at the 1% concentration. Relationships between metanil yellow spectral peak intensities and metanil yellow concentration were established using representative peaks at FT-Raman 1406 cm(-1) and FT-IR 1140 cm(-1) with correlation coefficients of 0.93 and 0.95, respectively. C1 [Dhakal, Sagar; Chao, Kuanglin; Schmidt, Walter; Qin, Jianwei; Kim, Moon; Chan, Diane] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr East, Bldg 303,10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Chao, KL (reprint author), USDA ARS, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr East, Bldg 303,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM sagar.dhakal@ars.usda.gov; kevin.chao@ars.usda.gov; walter.schmidt@ars.usda.gov; jianwei.qin@ars.usda.gov; klchao@hotmail.com; diane.chan@ars.usda.gov NR 65 TC 3 Z9 3 U1 6 U2 6 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2304-8158 J9 FOODS JI Foods PD JUN PY 2016 VL 5 IS 2 DI 10.3390/foods5020036 PG 15 WC Food Science & Technology SC Food Science & Technology GA DS6RT UT WOS:000380910700016 ER PT J AU Walker, BJ Skabelund, DC Busch, FA Ort, D AF Walker, Berkley J. Skabelund, Dane C. Busch, Florian A. Ort, DonaldR. TI An improved approach for measuring the impact of multiple CO2 conductances on the apparent photorespiratory CO2 compensation point through slope-intercept regression SO PLANT CELL AND ENVIRONMENT LA English DT Article DE FvCB photosynthesis model; mesophyll conductance; photosynthesis ID MESOPHYLL CONDUCTANCE; GAS-EXCHANGE; TEMPERATURE RESPONSE; PHOTOSYNTHESIS; C-3; LEAVES AB Biochemical models of leaf photosynthesis, which are essential for understanding the impact of photosynthesis to changing environments, depend on accurate parameterizations. One such parameter, the photorespiratory CO2 compensation point can bemeasured fromthe intersection of several CO2 response curves measured under sub-saturating illumination. However, determining the actual intersection while accounting for experimental noise can be challenging. Additionally, leaf photosynthesis model outcomes are sensitive to the diffusion paths of CO2 released from the mitochondria. This diffusion path of CO2 includes both chloroplastic as well as cell wall resistances to CO2, which are not readily measurable. Both the difficulties of determining the photorespiratory CO2 compensation point and the impact of multiple intercellular resistances to CO2 can be addressed through application of slope-intercept regression. This technical report summarizes an improved framework for implementing slope-intercept regression to evaluate measurements of the photorespiratory CO2 compensation point. This approach extends past work to include the cases of both Rubisco and Ribulose-1,5-bisphosphate (RuBP)-limited photosynthesis. This report further presents two interactive graphical applications and a spreadsheet-based tool to allow users to apply slope-intercept theory to their data. C1 [Walker, Berkley J.; Ort, DonaldR.] ARS, Global Change & Photosynth Res Unit, USDA, Urbana, IL 61801 USA. [Walker, Berkley J.; Ort, DonaldR.] Univ Illinois, Carl R Woese Inst Genom Biol, Urbana, IL 61801 USA. [Skabelund, Dane C.] Univ Illinois, Dept Math, Urbana, IL 61801 USA. [Busch, Florian A.] Australian Natl Univ, Res Sch Biol, GPO Box 4, Canberra, ACT 2601, Australia. [Ort, DonaldR.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. RP Ort, D (reprint author), ARS, Global Change & Photosynth Res Unit, USDA, Urbana, IL 61801 USA.; Ort, D (reprint author), Univ Illinois, Carl R Woese Inst Genom Biol, Urbana, IL 61801 USA.; Ort, D (reprint author), Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. EM d-ort@illinois.edu RI Busch, Florian/I-3816-2012 OI Busch, Florian/0000-0001-6912-0156 FU Bill and Melinda Gates Foundation [OPP1060461]; ARC Centre of Excellence for Translational Photosynthesis FX This research was supported via subcontract by the Bill and Melinda Gates Foundation (OPP1060461) titled 'RIPE-Realizing Increased Photosynthetic Efficiency for Sustainable Increases in Crop Yield'. F.A.B. is supported by the ARC Centre of Excellence for Translational Photosynthesis. NR 20 TC 1 Z9 1 U1 10 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7791 EI 1365-3040 J9 PLANT CELL ENVIRON JI Plant Cell Environ. PD JUN PY 2016 VL 39 IS 6 BP 1198 EP 1203 DI 10.1111/pce.12722 PG 6 WC Plant Sciences SC Plant Sciences GA DT5BL UT WOS:000381495500004 PM 27103099 ER PT J AU Attademo, FLN Ribeiro, VO Soares, HS Luna, FO Sousa, GP Freire, ACB Gennari, SM Alves, LC Marvulo, MFV Dubey, JP Silva, JCR AF Attademo, Fernanda L. N. Ribeiro, Vanessa O. Soares, Herbert S. Luna, Fabia O. Sousa, Glaucia P. Freire, Augusto C. B. Gennari, Solange M. Alves, Leucio C. Marvulo, Maria Fernanda V. Dubey, Jitender P. Silva, Jean C. R. TI SEROPREVALENCE OF TOXOPLASMA GONDII IN CAPTIVE ANTILLEAN MANATEE (TRICHECHUS MANATUS MANATUS) IN BRAZIL SO JOURNAL OF ZOO AND WILDLIFE MEDICINE LA English DT Article DE Antillean manatee; conservation medicine; marine mammals; modified agglutination test; Toxoplasma gondii; Trichechus manatus manatus ID SEA OTTERS; AMAZON; INFECTION; INUNGUIS; DOLPHINS; OOCYSTS AB Antillean manatees (Trichechus manatus manatus) are aquatic mammals that inhabit marine waters from Central America to the northeastern region of Brazil, and they are an endangered species. Infection with Toxoplasma gondii through intake of water or food contaminated with oocysts has been reported among marine mammals. The present study aimed to evaluate the prevalence of antibodies to T. gondii in West Indian manatees living in captivity in northeastern Brazil. Serum samples from 55 West Indian manatees from three different captive groups were tested for T. gondii antibodies by means of the modified agglutination test using a cutoff of 1:25. The samples were screened at dilutions of 1:25, 1:50, and 1:500, and positive samples were end-titrated using twofold serial dilutions; antibodies were found in six Antillean manatees (10.9%) with titers of 1:50 in three, 1: 500 in one, 1:3,200 in one, and 1:51,200 in one manatee. This study is the first report of T. gondii antibodies in captive Antillean manatees in Brazil. C1 [Attademo, Fernanda L. N.; Ribeiro, Vanessa O.; Alves, Leucio C.; Silva, Jean C. R.] Univ Fed Rural Pernambuco, Dept Vet Med, Rua Dom Manoel de Medeiros S-N, BR-52171900 Recife, PE, Brazil. [Attademo, Fernanda L. N.; Luna, Fabia O.; Sousa, Glaucia P.] Ctr Nacl Pesquisa & Conservacao Mamiferos Aquat, Inst Chico Mendes Conservacao Biodiversidade, Estr Forte Orange S-N, BR-53900000 Ilha De Itamaraca, PE, Brazil. [Freire, Augusto C. B.] Inst Mamiferos Aquat, Estr Forte Orange S-N,Caixa Postal 01, BR-53900000 Ilha De Itamaraca, PE, Brazil. [Attademo, Fernanda L. N.; Ribeiro, Vanessa O.; Marvulo, Maria Fernanda V.; Silva, Jean C. R.] Inst Brasileiro Med Conservacao Triade, Rua Silveira Lobo 32,Caixa Postal 48, BR-52061030 Recife, PE, Brazil. [Soares, Herbert S.; Gennari, Solange M.] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, Ave Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, Brazil. [Marvulo, Maria Fernanda V.] Fac Max Planck, Rodovia Joao Ceccon 60, BR-13331400 Sao Paulo, Brazil. [Marvulo, Maria Fernanda V.] Univ Paulista, Ave Comendador Enzo Ferrari 280, BR-13043900 Sao Paulo, Brazil. [Dubey, Jitender P.] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Bldg 1001, Beltsville, MD 20705 USA. RP Attademo, FLN (reprint author), Univ Fed Rural Pernambuco, Dept Vet Med, Rua Dom Manoel de Medeiros S-N, BR-52171900 Recife, PE, Brazil.; Attademo, FLN (reprint author), Ctr Nacl Pesquisa & Conservacao Mamiferos Aquat, Inst Chico Mendes Conservacao Biodiversidade, Estr Forte Orange S-N, BR-53900000 Ilha De Itamaraca, PE, Brazil.; Attademo, FLN (reprint author), Inst Brasileiro Med Conservacao Triade, Rua Silveira Lobo 32,Caixa Postal 48, BR-52061030 Recife, PE, Brazil. EM niemeyerattademo@yahoo.com.br FU National Center for Scientific and Technological Development FX The authors thank the staff of the Brazilian National Center of Research and Conservation of Aquatic Mammals of Chico Mendes Institute for Biodiversity Conservation. Funding was provided by the National Center for Scientific and Technological Development. S.M.G., L.C.A., and J.C.R.S. received a fellowship from the National Center for Scientific and Technological Development. NR 21 TC 0 Z9 0 U1 7 U2 7 PU AMER ASSOC ZOO VETERINARIANS PI YULEE PA 581705 WHITE OAK ROAD, YULEE, FL 32097 USA SN 1042-7260 EI 1937-2825 J9 J ZOO WILDLIFE MED JI J. Zoo Wildl. Med. PD JUN PY 2016 VL 47 IS 2 BP 423 EP 426 PG 4 WC Veterinary Sciences SC Veterinary Sciences GA DS5PI UT WOS:000380834100005 PM 27468012 ER PT J AU Flitcroft, RL Bottom, DL Haberman, KL Bierly, KF Jones, KK Simenstad, CA Gray, A Ellingson, KS Baumgartner, E Cornwell, TJ Campbell, LA AF Flitcroft, Rebecca L. Bottom, Daniel L. Haberman, Karen L. Bierly, Ken F. Jones, Kim K. Simenstad, Charles A. Gray, Ayesha Ellingson, Kami S. Baumgartner, Erin Cornwell, Trevan J. Campbell, Lance A. TI Expect the unexpected: place-based protections can lead to unforeseen benefits SO AQUATIC CONSERVATION-MARINE AND FRESHWATER ECOSYSTEMS LA English DT Article ID SALMON RIVER ESTUARY; FISH GROWTH; OREGON; RESTORATION; RESIDENCY; USA; RESILIENCE; ECOSYSTEMS; HABITAT; HISTORY AB 1. Protection of places important for aesthetic, ecological, and cultural values has been a goal of conservationists for over 150 years. Cornerstones of place-based conservation include legal designations, international agreements, and purchase by public or non-profit organizations. 2. In the Salmon River catchment, Oregon, protections were initially developed in the 1930s for the freshwater riparian corridor and forestry research in the uplands. Over time, additional protections in the estuary and nearshore marine environments were added, motivated by local desire to protect and restore habitats and fish populations. 3. Removal of three levees in the Salmon River estuary occurred over three consecutive 9-year time-steps, and provided the opportunity for research on tidal marsh recovery in the framework of a space-for-time chronoseries. Elevation, channel morphology, and vegetation all exhibited trajectories toward reference conditions. Fish and macroinvertebrates also served as indicators of tidal marsh recovery, although their recovery patterns were not strictly related to the chrono-series trajectories. The extent of restoration provided a novel opportunity to measure a significant response of biotic indicators at the site and catchment scales. 4. Salt marsh restoration augmented protected freshwater habitats by expanding rearing habitats for juvenile salmonids and increasing expression of life-history diversity for both Chinook and coho salmon. This finding highlights linkages between freshwater and marine habitats and populations, and has the potential to influence important policy advances and changes in management of Pacific salmon. 5. Restoration promoted collaborations among stakeholders, community involvement, and inspiring educational opportunities that enabled more comprehensive research than any single sponsor could have accomplished. 6. Protected status designations have fostered a wealth of opportunities that were not specifically envisioned when the protections were first put in place. In particular, dedicated scientific investigation of landscape-scale change did not occur by design, but was pieced together as funding opportunities arose over time. Copyright (C) 2016 John Wiley & Sons, Ltd. C1 [Flitcroft, Rebecca L.] US Forest Serv, USDA, Res Lab, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. [Bottom, Daniel L.] NOAA, Natl Marine Fisheries Serv, Northwest Fisheries Sci Ctr, Div Ecol, Seattle, WA 98115 USA. [Haberman, Karen L.; Baumgartner, Erin] Western Oregon Univ, Div Nat Sci & Math, Monmouth, OR USA. [Bierly, Ken F.] Bierly & Associates, Salem, OR USA. [Jones, Kim K.; Cornwell, Trevan J.] Oregon Dept Fish & Wildlife, Conservat & Recovery Sect, Corvallis, OR USA. [Simenstad, Charles A.] Univ Washington, Sch Aquat & Fishery Sci, Seattle, WA 98195 USA. [Gray, Ayesha] Mississippi Dept Marine Resources, Grand Bay Natl Estuarine Res Reserve, Moss Point, MS USA. [Ellingson, Kami S.] US Forest Serv, USDA, Siuslaw Natl Forest, Corvallis, OR USA. [Campbell, Lance A.] Oregon State Univ, Hatfield Marine Sci Ctr, Coastal Oregon Marine Expt Stn, Newport, OR 97365 USA. [Campbell, Lance A.] Oregon State Univ, Hatfield Marine Sci Ctr, Dept Fisheries & Wildlife, Newport, OR 97365 USA. RP Flitcroft, RL (reprint author), US Forest Serv, USDA, Res Lab, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM rflitcroft@fs.fed.us FU Oregon Sea Grant; Oregon Watershed Enhancement Board; National Science Foundation; USFS; ODFW FX Some extremely dedicated individuals looked at the Salmon River catchment as a unique research opportunity, beginning with Bob Frenkel of Oregon State University starting in the late 1970s. In many instances they worked at Salmon River despite institutional resistance and lack of dedicated funding. Funding gaps were filled by a suite of organizations, most notably Oregon Sea Grant, but also the Oregon Watershed Enhancement Board, National Science Foundation, USFS, and the ODFW. The dedication by graduate students and undergraduates who have participated in the research in the Salmon River catchment has been instrumental in data collection, analyses, and developing research topics and interests. NR 50 TC 3 Z9 3 U1 9 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1052-7613 EI 1099-0755 J9 AQUAT CONSERV JI Aquat. Conserv.-Mar. Freshw. Ecosyst. PD JUN PY 2016 VL 26 SU 1 BP 39 EP 59 DI 10.1002/aqc.2660 PG 21 WC Environmental Sciences; Marine & Freshwater Biology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA DT2GL UT WOS:000381298700004 ER PT J AU Juffe-Bignoli, D Harrison, I Butchart, SHM Flitcroft, R Hermoso, V Jonas, H Lukasiewicz, A Thieme, M Turak, E Bingham, H Dalton, J Darwall, W Deguignet, M Dudleyo, N Gardner, R Higgins, J Kumar, R Linke, S Milton, GR Pittock, J Smith, KG Van Soesbergen, A AF Juffe-Bignoli, Diego Harrison, Ian Butchart, Stuart H. M. Flitcroft, Rebecca Hermoso, Virgilio Jonas, Harry Lukasiewicz, Anna Thieme, Michele Turak, Eren Bingham, Heather Dalton, James Darwall, William Deguignet, Marine Dudleyo, Nigel Gardner, Royal Higgins, Jonathan Kumar, Ritesh Linke, Simon Milton, G. Randy Pittock, Jamie Smith, Kevin G. Van Soesbergen, Arnout TI Achieving Aichi Biodiversity Target 11 to improve the performance of protected areas and conserve freshwater biodiversity SO AQUATIC CONSERVATION-MARINE AND FRESHWATER ECOSYSTEMS LA English DT Article DE protected areas; freshwater biodiversity; Convention on Biological Diversity; Aichi Biodiversity Targets; Ramsar Convention ID CLIMATE-CHANGE; RIVER-BASIN; ECOSYSTEM SERVICES; GLOBAL ASSESSMENT; FRAMEWORK; SYSTEMS; CONNECTIVITY; CHALLENGES; PROGRESS; WETLAND AB 1. The Strategic Plan for Biodiversity (2011-2020), adopted at the 10th meeting of the Conference of the Parties to the Convention on Biological Diversity, sets 20 Aichi Biodiversity Targets to be met by 2020 to address biodiversity loss and ensure its sustainable and equitable use. Aichi Biodiversity Target 11 describes what an improved conservation network would look like for marine, terrestrial and inland water areas, including freshwater ecosystems. 2. To date, there is no comprehensive assessment of what needs to be achieved to meet Target 11 for freshwater biodiversity. Reports on implementation often fail to consider explicitly freshwater ecosystem processes and habitats, the pressures upon them, and therefore the full range of requirements and actions needed to sustain them. 3. Here the current progress and key gaps for meeting Aichi Target 11 are assessed by exploring the implications of each of its clauses for freshwater biodiversity. 4. Concerted action on Aichi Biodiversity Target 11 for freshwater biodiversity by 2020 is required in a number of areas: a robust baseline is needed for each of the clauses described here at national and global scales; designation of new protected areas or expansion of existing protected areas to cover known areas of importance for biodiversity and ecosystem services, and a representative sample of biodiversity; use of Other Effective Area-Based Conservation Measures (OECMs) in places where designating a protected area is not appropriate; and promoting and implementing better management strategies for fresh water in protected areas that consider its inherent connectivity, contextual vulnerability, and required human and technical capacity. 5. Considering the specific requirements of freshwater systems through Aichi Biodiversity Target 11 has long-term value to the Sustainable Development Goals discussions and global conservation policy agenda into the coming decades. Copyright (C) 2016 John Wiley & Sons, Ltd. C1 [Juffe-Bignoli, Diego; Bingham, Heather; Deguignet, Marine; Van Soesbergen, Arnout] United Nations Environm Programme World Conservat, 219 Huntingdon Rd, Cambridge CB3 0DL, England. [Harrison, Ian] Int Union Conservat Nat Species Survival Commiss, Arlington, VA USA. [Harrison, Ian] Conservat Int, Arlington, VA USA. [Butchart, Stuart H. M.] BirdLife Int, Cambridge, England. [Butchart, Stuart H. M.] Univ Cambridge, Dept Zool, Cambridge, England. [Flitcroft, Rebecca] US Forest Serv, USDA, Pacific NW Res Stn, Corvallis, OR USA. [Hermoso, Virgilio] Ctr Tecnol Forestal Catalunya, Crta St Llorenc Morunys, Solsona, Lleida, Spain. [Jonas, Harry] Nat Justice Lawyers Commun & Environm, Cape Town, Western Cape, South Africa. [Pittock, Jamie] Australian Natl Univ, Fenner Sch Environm & Soc, Canberra, ACT, Australia. [Thieme, Michele] World Wildlife Fund, 1250 24th St,NW, Washington, DC 20037 USA. [Turak, Eren] NSW Off Environm & Heritage, Sydney, NSW, Australia. [Lukasiewicz, Anna; Turak, Eren] Australian Museum, Sydney, NSW, Australia. [Dalton, James] Int Union Conservat Nat, Global Water Programme, Gland, Switzerland. [Darwall, William; Smith, Kevin G.] Int Union Conservat Nat, Global Species Programme, Cambridge, England. [Dudleyo, Nigel] Equilibrium Res, Bristol, Avon, England. [Dudleyo, Nigel] Univ Queensland, Sch Geog, Planning & Environm Management, Brisbane, Qld, Australia. [Gardner, Royal] Stetson Univ, Coll Law, Gulfport, FL USA. [Higgins, Jonathan] Nature Conservancy, 1815 N Lynn St, Arlington, VA USA. [Kumar, Ritesh] Wetlands Int South Asia, New Delhi, India. [Linke, Simon] Griffith Univ, Australian Rivers Inst, Nathan, Qld, Australia. [Milton, G. Randy] Nova Scotia Dept Nat Resources, Kentville, NS, Canada. RP Juffe-Bignoli, D (reprint author), United Nations Environm Programme World Conservat, 219 Huntingdon Rd, Cambridge CB3 0DL, England. EM diego.juffe-bignoli@unep-wcmc.org FU Ramon y Cajal contract - Spanish government [RYC-2013-13979]; ARC DECRA [DE130100565] FX This manuscript was developed in part from discussions held at Stream 1 ('Reaching Conservation Goals') workshops held at the 2014 World Parks Congress. We are grateful to all present in those workshops for developing stimulating conversations, and to the organizers of Stream 1 for providing the opportunity to hold those workshops. DJB would like to thank Naomi Kingston and Brian MacSharry for their support in developing this manuscript. VH was funded by a Ramon y Cajal contract (RYC-2013-13979) funded by the Spanish government. IH is grateful to Conservation International for funding his contributions to the 2014 World Parks Congress; the Department of Ichthyology, American Museum of Natural History, New York for granting Research Associate status; and Columbia University, New York for granting Adjunct Research Scientist Status, and allowing access to their library facilities. SL was funded by an ARC DECRA grant DE130100565. Finally, we thank the many thousands of individuals and organizations who contribute to the IUCN Red List assessments for freshwater taxa, and the identification of IBAs, AZEs and other KBAs for freshwater taxa, which underpin some of the analyses presented here. NR 124 TC 2 Z9 2 U1 13 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1052-7613 EI 1099-0755 J9 AQUAT CONSERV JI Aquat. Conserv.-Mar. Freshw. Ecosyst. PD JUN PY 2016 VL 26 SU 1 BP 133 EP 151 DI 10.1002/aqc.2638 PG 19 WC Environmental Sciences; Marine & Freshwater Biology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA DT2GL UT WOS:000381298700010 ER PT J AU Li, T Luo, M Zhang, DD Wu, D Li, L Bai, GH AF Li, Tao Luo, Meng Zhang, Dadong Wu, Di Li, Lei Bai, Guihua TI Effective marker alleles associated with type 2 resistance to Fusarium head blight infection in fields SO BREEDING SCIENCE LA English DT Article DE wheat; Fusarium head blight; breeding population; association analysis; molecular markers; allelic effects ID QUANTITATIVE TRAIT LOCI; TRITICUM-AESTIVUM L.; WINTER-WHEAT; MAJOR QTL; POPULATION; BARLEY; FHB1; LINES; GENE AB Molecular markers associated with known quantitative trait loci (QTLs) for type 2 resistance to Fusarium head blight (FHB) in bi-parental mapping population usually have more than two alleles in breeding populations. Therefore, understanding the association of each allele with FHB response is particularly important to marker-assisted enhancement of FHB resistance. In this paper, we evaluated FHB severities of 192 wheat accessions including landraces and commercial varieties in three field growing seasons, and genotyped this panel with 364 genome-wide informative molecular markers. Among them, 11 markers showed reproducible marker-trait association (p < 0.05) in at least two experiments using a mixed model. More than two alleles were identified per significant marker locus. These alleles were classified into favorable, unfavorable and neutral alleles according to the normalized genotypic values. The distributions of effective alleles at these loci in each wheat accession were characterized. Mean FHB severities increased with decreased number of favorable alleles at the reproducible loci. Chinese wheat landraces and Japanese accessions have more favorable alleles at the majority of the reproducible marker loci. FHB resistance levels of varieties can be greatly improved by introduction of these favorable alleles and removal of unfavorable alleles simultaneously at these QTL-linked marker loci. C1 [Li, Tao; Luo, Meng; Wu, Di; Li, Lei] Yangzhou Univ, Coinnovat Ctr Modern Prod Technol Grain Crops, Wheat Res Ctr,Minist Educ, Key Lab Plant Funct Genom,Jiangsu Prov Key Lab Cr, Yangzhou 225009, Jiangsu, Peoples R China. [Zhang, Dadong; Bai, Guihua] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Bai, Guihua] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. RP Li, T (reprint author), Yangzhou Univ, Coinnovat Ctr Modern Prod Technol Grain Crops, Wheat Res Ctr,Minist Educ, Key Lab Plant Funct Genom,Jiangsu Prov Key Lab Cr, Yangzhou 225009, Jiangsu, Peoples R China. EM taoli@yzu.edu.cn FU National Major Project of Breeding for New Transgenic Organisms [2012ZX08009003-004]; NSFC [31171537]; Innovative Research Team of Universities in Jiangsu Province; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) FX We acknowledge financial support by the National Major Project of Breeding for New Transgenic Organisms (2012ZX08009003-004), NSFC (31171537), the Innovative Research Team of Universities in Jiangsu Province, and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). NR 36 TC 0 Z9 1 U1 7 U2 7 PU JAPANESE SOC BREEDING PI TOKYO PA UNIV TOKYO, GRADUATE SCHOOL AGRICULTURAL LIFE SCIENCES, BUNKYO-KU, TOKYO, 113-8657, JAPAN SN 1344-7610 EI 1347-3735 J9 BREEDING SCI JI Breed. Sci. PD JUN PY 2016 VL 66 IS 3 BP 350 EP 357 DI 10.1270/jsbbs.15124 PG 8 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DS2PL UT WOS:000380625900002 PM 27436944 ER PT J AU Seibold, S Bassler, C Brandl, R Buche, B Szallies, A Thorn, S Ulyshen, MD Muller, J AF Seibold, Sebastian Baessler, Claus Brandl, Roland Bueche, Boris Szallies, Alexander Thorn, Simon Ulyshen, Michael D. Mueller, Joerg TI Microclimate and habitat heterogeneity as the major drivers of beetle diversity in dead wood SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE dead-wood enrichment; experiment; forest conservation; forest restoration; habitat-heterogeneity hypothesis; more-individuals hypothesis; saproxylic beetles; species-energy hypothesis; woody debris ID SPECIES-DIVERSITY; SAPROXYLIC BEETLES; BEECH FORESTS; BIODIVERSITY; MANAGEMENT; COMMUNITIES; ASSEMBLAGES; ENERGY; TEMPERATURE; INSECTS AB 1. Resource availability and habitat heterogeneity are principle drivers of biodiversity, but their individual roles often remain unclear since both factors are usually correlated. The biodiversity of species dependent on dead wood could be driven by either resource availability represented by dead-wood amount or habitat heterogeneity characterized by dead-wood diversity or both. Understanding their roles is crucial for improving evidence-based conservation strategies for saproxylic species in managed forests. 2. To disentangle the effects of dead-wood amount and dead-wood diversity on biodiversity relative to canopy openness (microclimate), we experimentally exposed different amounts of logs and branches of two different tree species representing a gradient of dead-wood diversity in 190 sunny and shady forest plots. During the 3 years after exposing dead wood, we sampled saproxylic beetles, which are together with fungi the most diverse and important taxonomic group involved in decomposition of wood. 3. The composition of saproxylic beetle assemblages differed clearly between shady and sunny forest plots, with higher richness in sunny plots. Both dead-wood amount and deadwood diversity positively and independently affected species richness of saproxylic beetles, but these effects were mediated by canopy openness. In sunny forest, species richness increased with increasing amount of dead wood, whereas in shady forest, dead-wood diversity was the prevailing factor. 4. The stepwise analysis of abundance and species richness, however, indicated that effects of both factors supported only the habitat-heterogeneity hypothesis, as the positive effect of high amounts of dead wood could be explained by cryptic variability of dead-wood quality within single objects. 5. Synthesis and applications. As canopy openness and habitat heterogeneity seem to be the major drivers of saproxylic beetle diversity in temperate forests, we recommend that managers aim to increase the heterogeneity of dead-wood substrates under both sunny and shady forest conditions. Intentional opening of the canopy should be considered in anthropogenically homogenized, dense forests. Specifically in temperate mixed montane forests, dead wood should be provided in the form of large logs in sunny habitats and a high diversity of different dead-wood substrates should be retained or created in shady forests. C1 [Seibold, Sebastian; Baessler, Claus; Thorn, Simon; Mueller, Joerg] Bavarian Forest Natl Pk,Freyunger Str 2, D-94481 Grafenau, Germany. [Seibold, Sebastian; Thorn, Simon; Mueller, Joerg] Tech Univ Munich, Dept Ecol & Ecosyst Management, Ctr Food & Life Sci Weihenstephan, Terr Ecol Res Grp, Hans Carl von Carlowitz Pl 2, D-85354 Freising Weihenstephan, Germany. [Brandl, Roland] Univ Marburg, Fac Biol, Dept Ecol, Anim Ecol, Karl von Frisch Str 8, D-35032 Marburg, Germany. [Szallies, Alexander] ZHAW, IUNR, CH-8820 Wadenswil, Switzerland. [Ulyshen, Michael D.] US Forest Serv, USDA, Southern Res Stn, Athens, GA 30602 USA. RP Seibold, S (reprint author), Bavarian Forest Natl Pk,Freyunger Str 2, D-94481 Grafenau, Germany.; Seibold, S (reprint author), Tech Univ Munich, Dept Ecol & Ecosyst Management, Ctr Food & Life Sci Weihenstephan, Terr Ecol Res Grp, Hans Carl von Carlowitz Pl 2, D-85354 Freising Weihenstephan, Germany. EM sebastian-seibold@gmx.de FU German Federal Ministry for Education and Research [01LC1323A]; European Regional Development Fund (ERDF); German Federal Environmental Foundation FX We are grateful to all those who helped in the field and in the laboratory to set up and conduct the experiment, especially O. Schubert, T. Bauer and the staff of the Bavarian Forest National Park. We thank K. A. Brune for linguistic revision of the manuscript. This study was supported by the German Federal Ministry for Education and Research through the project BioHolz (no. 01LC1323A) and the European Regional Development Fund (ERDF). S.S. and S.T. were supported by the Scholarship Program of the German Federal Environmental Foundation. NR 59 TC 6 Z9 6 U1 23 U2 30 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD JUN PY 2016 VL 53 IS 3 BP 934 EP 943 DI 10.1111/1365-2664.12607 PG 10 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DR7FM UT WOS:000380065400033 ER PT J AU O'Neil, GW Williams, JR Wilson-Peltier, J Knothe, G Reddy, CM AF O'Neil, Gregory W. Williams, John R. Wilson-Peltier, Julia Knothe, Gerhard Reddy, Christopher M. TI Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass SO JOVE-JOURNAL OF VISUALIZED EXPERIMENTS LA English DT Article DE Environmental Sciences; Issue 112; Algal biofuels; biodiesel; fatty esters; lipids; coproducts; alkenones; Isochrysis ID EMILIANIA-HUXLEYI; GEPHYROCAPSA-OCEANICA; CANOLA OIL; MICROALGAE; CALIBRATION; GALBANA; LIPIDS; FUEL AB The need to replace petroleum fuels with alternatives from renewable and more environmentally sustainable sources is of growing importance. Biomass-derived biofuels have gained considerable attention in this regard, however first generation biofuels from edible crops like corn ethanol or soybean biodiesel have generally fallen out of favor. There is thus great interest in the development of methods for the production of liquid fuels from domestic and superior non-edible sources. Here we describe a detailed procedure for the production of a purified biodiesel from the marine microalgae Isochrysis. Additionally, a unique suite of lipids known as polyunsaturated long-chain alkenones are isolated in parallel as potentially valuable coproducts to offset the cost of biodiesel production. Multi-kilogram quantities of Isochrysis are purchased from two commercial sources, one as a wet paste (80% water) that is first dried prior to processing, and the other a dry milled powder (95% dry). Lipids are extracted with hexanes in a Soxhlet apparatus to produce an algal oil ("hexane algal oil") containing both traditional fats (i.e., triglycerides, 46-60% w/w) and alkenones (16-25% w/w). Saponification of the triglycerides in the algal oil allows for separation of the resulting free fatty acids (FFAs) from alkenone-containing neutral lipids. FFAs are then converted to biodiesel (i.e., fatty acid methyl esters, FAMEs) by acid-catalyzed esterification while alkenones are isolated and purified from the neutral lipids by crystallization. We demonstrate that biodiesel from both commercial Isochrysis biomasses have similar but not identical FAME profiles, characterized by elevated polyunsaturated fatty acid contents (approximately 40% w/w). Yields of biodiesel were consistently higher when starting from the Isochrysis wet paste (12% w/w vs. 7% w/w), which can be traced to lower amounts of hexane algal oil obtained from the powdered Isochrysis product. C1 [O'Neil, Gregory W.; Williams, John R.; Wilson-Peltier, Julia] Western Washington Univ, Dept Chem, Bellingham, WA 98225 USA. [Knothe, Gerhard] ARS, USDA, Washington, DC USA. [Reddy, Christopher M.] Woods Hole Oceanog Inst, Dept Marine Chem & Geochem, Woods Hole, MA USA. RP O'Neil, GW (reprint author), Western Washington Univ, Dept Chem, Bellingham, WA 98225 USA. EM oneilg@chem.wwu.edu FU National Science Foundation [CHE-1151492]; Northwest Advanced Renewables Alliance FX This work was supported by the National Science Foundation (CHE-1151492), the Northwest Advanced Renewables Alliance (fellowship to J. Wilson-Peltier), and through a private donation from friends of WHOI. We thank Kevin R. Steidley and Kim Ascherl (USDA / ARS / NCAUR) for excellent technical assistance. NR 36 TC 0 Z9 0 U1 8 U2 10 PU JOURNAL OF VISUALIZED EXPERIMENTS PI CAMBRIDGE PA 1 ALEWIFE CENTER, STE 200, CAMBRIDGE, MA 02140 USA SN 1940-087X J9 JOVE-J VIS EXP JI J. Vis. Exp. PD JUN PY 2016 IS 112 AR e54189 DI 10.3791/54189 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DS0CX UT WOS:000380264100082 ER PT J AU Saporito, LS Bryant, RB Kleinman, PJA AF Saporito, Louis S. Bryant, Ray B. Kleinman, Peter J. A. TI A Protocol for Collecting and Constructing Soil Core Lysimeters SO JOVE-JOURNAL OF VISUALIZED EXPERIMENTS LA English DT Article DE Environmental Sciences; Issue 112; Lysimeter; Leaching; Soil Core; Water Quality; Agriculture; Soil; Percolation; Nonpoint Source; Nitrate ID MANURE APPLICATION; PHOSPHORUS LOSSES; POULTRY LITTER; SURFACE RUNOFF; NITRATE; SUBSURFACE; MONOLITHS; TOPSOILS; DESIGN; FLOW AB Leaching of nutrients from land applied fertilizers and manure used in agriculture can lead to accelerated eutrophication of surface water. Because the landscape has complex and varied soil morphology, an accompanying disparity in flow paths for leachate through the soil macropore and matrix structure is present. The rate of flow through these paths is further affected by antecedent soil moisture. Lysimeters are used to quantify flow rate, volume of water and concentration of nutrients leaching downward through soils. While many lysimeter designs exist, accurately determining the volume of water and mass balance of nutrients is best accomplished with bounded lysimeters that leave the natural soil structure intact. Here we present a detailed method for the extraction and construction of soil core lysimeters equipped with soil moisture sensors at 5 cm and 25 cm depths. Lysimeters from four different Coastal Plain soils (Bojac, Evesboro, Quindocqua and Sassafras) were collected on the Delmarva Peninsula and moved to an indoor climate controlled facility. Soils were irrigated once weekly with the equivalent of 2 cm of rainfall to draw down soil nitrate-N concentrations. At the end of the draw down period, poultry litter was applied (162 kg TN ha(-1)) and leaching was resumed for an additional five weeks. Total recovery of applied irrigation water varied from 71% to 85%. Nitrate-N concentration varied over the course of the study from an average of 27.1 mg L-1 before litter application to 40.3 mg L-1 following litter application. While greatest flux of nutrients was measured in soils dominated by coarse sand (Sassafras) the greatest immediate flux occurred from the finest textured soil with pronounced macropore development (Quindocqua). C1 [Saporito, Louis S.; Bryant, Ray B.; Kleinman, Peter J. A.] USDA ARS, Pasture Syst & Watershed Management Res Unit, Washington, DC 20250 USA. RP Saporito, LS (reprint author), USDA ARS, Pasture Syst & Watershed Management Res Unit, Washington, DC 20250 USA. EM Lou.Saporito@ars.usda.gov NR 30 TC 0 Z9 0 U1 4 U2 7 PU JOURNAL OF VISUALIZED EXPERIMENTS PI CAMBRIDGE PA 1 ALEWIFE CENTER, STE 200, CAMBRIDGE, MA 02140 USA SN 1940-087X J9 JOVE-J VIS EXP JI J. Vis. Exp. PD JUN PY 2016 IS 112 AR e53952 DI 10.3791/53952 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DS0CX UT WOS:000380264100031 ER PT J AU Gribok, A Leger, JL Stevens, M Hoyt, R Buller, M Rumpler, W AF Gribok, Andrei Leger, Jayme L. Stevens, Michelle Hoyt, Reed Buller, Mark Rumpler, William TI Measuring the short-term substrate utilization response to high-carbohydrate and high-fat meals in the whole-body indirect calorimeter SO PHYSIOLOGICAL REPORTS LA English DT Article DE Indirect calorimetry; metabolic flexibility; regularization ID METABOLIC FLEXIBILITY; GAS-EXCHANGE; ANAEROBIC THRESHOLD; TRANSIENT-RESPONSE; INSULIN-RESISTANCE; SKELETAL-MUSCLE; CO2 OUTPUT; EXERCISE; GLUCOSE; HYPERVENTILATION AB The paper demonstrates that minute-to-minute metabolic response to meals with different macronutrient content can be measured and discerned in the whole-body indirect calorimeter. The ability to discriminate between high-carbohydrate and high-fat meals is achieved by applying a modified regularization technique with additional constraints imposed on oxygen consumption rate. These additional constraints reduce the differences in accuracy between the oxygen and carbon dioxide analyzers. The modified technique was applied to 63 calorimeter sessions that were each 24 h long. The data were collected from 16 healthy volunteers (eight males, eight females, aged 22-35 years). Each volunteer performed four 24-h long calorimeter sessions. At each session, they received one of four treatment combinations involving exercise (high or low intensity) and diet (a high-fat or high-carbohydrate shake for lunch). One volunteer did not complete all four assignments, which brought the total number of sessions to 63 instead of 64. During the 24-h stay in the calorimeter, subjects wore a continuous glucose monitoring system, which was used as a benchmark for subject's postprandial glycemic response. The minute-by-minute respiratory exchange ratio (RER) data showed excellent agreement with concurrent subcutaneous glucose concentrations in postprandial state. The averaged minute-to-minute RER response to the high-carbohydrate shake was significantly different from the response to high-fat shake. Also, postprandial RER slopes were significantly different for two dietary treatments. The results show that whole-body respiration calorimeters can be utilized as tools to study short-term kinetics of substrate oxidation in humans. C1 [Gribok, Andrei; Leger, Jayme L.; Stevens, Michelle; Rumpler, William] ARS, Food Components & Hlth Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD USA. [Hoyt, Reed; Buller, Mark] US Army Res Inst Environm Med, Biophys & Biomed Modeling Div, Natick, MA USA. RP Gribok, A (reprint author), ARS, Food Components & Hlth Lab, USDA, 10300 Baltimore Ave,Bld 307B,Room 217, Beltsville, MD 20705 USA. EM agribok@utk.edu NR 35 TC 0 Z9 0 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2051-817X J9 PHYSIOL REP JI PHYSIOL. REP. PD JUN PY 2016 VL 4 IS 12 AR e12835 DI 10.14814/phy2.12835 PG 12 WC Physiology SC Physiology GA DS0AN UT WOS:000380257600009 ER PT J AU Knipfer, T Cuneo, IF Brodersen, CR McElrone, AJ AF Knipfer, Thorsten Cuneo, Italo F. Brodersen, Craig R. McElrone, Andrew J. TI In Situ Visualization of the Dynamics in Xylem Embolism Formation and Removal in the Absence of Root Pressure: A Study on Excised Grapevine Stems SO PLANT PHYSIOLOGY LA English DT Article ID X-RAY MICROTOMOGRAPHY; VITIS-VINIFERA; LAURUS-NOBILIS; VULNERABILITY CURVES; VIVO VISUALIZATIONS; WATER-STRESS; CAVITATION; VESSELS; REPAIR; DROUGHT AB Gas embolisms formed during drought can disrupt long-distance water transport through plant xylem vessels, but some species have the ability to remove these blockages. Despite evidence suggesting that embolism removal is linked to the presence of vessel-associated parenchyma, the underlying mechanism remains controversial and is thought to involve positive pressure generated by roots. Here, we used in situ x-ray microtomography on excised grapevine stems to determine if embolism removal is possible without root pressure, and if the embolism formation/removal affects vessel functional status after sample excision. Our data show that embolism removal in excised stems was driven by water droplet growth and was qualitatively identical to refilling in intact plants. When stem segments were rehydrated with H2O after excision, vessel refilling occurred rapidly (<1 h). The refilling process was substantially slower when polyethylene glycol was added to the H2O source, thereby providing new support for an osmotically driven refilling mechanism. In contrast, segments not supplied with H2O showed no refilling and increased embolism formation. Dynamic changes in liquid/wall contact angles indicated that the processes of embolism removal (i.e. vessel refilling) by water influx and embolism formation by water efflux were directly linked to the activity of vessel-associated living tissue. Overall, our results emphasize that root pressure is not required as a driving force for vessel refilling, and care should be taken when performing hydraulics measurements on excised plant organs containing living vessel-associated tissue, because the vessel behavior may not be static. C1 [Knipfer, Thorsten; Cuneo, Italo F.; McElrone, Andrew J.] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. [Cuneo, Italo F.] Pontificia Univ Catolica Valparaiso, Sch Agron, Quillota, Chile. [Brodersen, Craig R.] Yale Univ, Sch Forestry & Environm Studies, New Haven, CT 06511 USA. [McElrone, Andrew J.] USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA 95618 USA. RP Knipfer, T; McElrone, AJ (reprint author), Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. EM knipfer.thorsten@yahoo.de; ajmcelrone@ucdavis.edu RI Brodersen, Craig/J-1112-2016 OI Brodersen, Craig/0000-0002-0924-2570 FU National Institute of Food and Agriculture Specialty Crop Research Initiative grant; United States Department of Agriculture-Agricultural Research Service Current Research Information System [5306-21220-004-00]; US Department of Energy [DE-AC02-05CH11231] FX This work was supported by a National Institute of Food and Agriculture Specialty Crop Research Initiative grant and funding from the American Vineyard Foundation to A.J.M., and United States Department of Agriculture-Agricultural Research Service Current Research Information System funding under grant no. 5306-21220-004-00. The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Science, of the US Department of Energy under contract no. DE-AC02-05CH11231. NR 56 TC 6 Z9 6 U1 11 U2 18 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD JUN PY 2016 VL 171 IS 2 BP 1024 EP 1036 DI 10.1104/pp.16.00136 PG 13 WC Plant Sciences SC Plant Sciences GA DS3RA UT WOS:000380699200022 PM 27208267 ER PT J AU Cook, SD Nichols, DS Smith, J Chourey, PS McAdam, EL Quittenden, L Ross, JJ AF Cook, Sam D. Nichols, David S. Smith, Jason Chourey, Prem S. McAdam, Erin L. Quittenden, Laura Ross, John J. TI Auxin Biosynthesis: Are the Indole-3-Acetic Acid and Phenylacetic Acid Biosynthesis Pathways Mirror Images? SO PLANT PHYSIOLOGY LA English DT Article ID PHENYLALANINE BIOSYNTHESIS; ARABIDOPSIS-THALIANA; PREPHENATE AMINOTRANSFERASE; REPRODUCTIVE DEVELOPMENT; AZOSPIRILLUM-BRASILENSE; MASS-SPECTROMETRY; GENE-EXPRESSION; IN-VIVO; TRYPTOPHAN; PLANTS AB The biosynthesis of the main auxin in plants (indole-3-acetic acid [IAA]) has been elucidated recently and is thought to involve the sequential conversion of Trp to indole-3-pyruvic acid to IAA. However, the pathway leading to a less well studied auxin, phenylacetic acid (PAA), remains unclear. Here, we present evidence from metabolism experiments that PAA is synthesized from the amino acid Phe, via phenylpyruvate. In pea (Pisum sativum), the reverse reaction, phenylpyruvate to Phe, is also demonstrated. However, despite similarities between the pathways leading to IAA and PAA, evidence from mutants in pea and maize (Zea mays) indicate that IAA biosynthetic enzymes are not the main enzymes for PAA biosynthesis. Instead, we identified a putative aromatic aminotransferase (PsArAT) from pea that may function in the PAA synthesis pathway. C1 [Cook, Sam D.; McAdam, Erin L.; Quittenden, Laura; Ross, John J.] Univ Tasmania, Sch Biol Sci, Sandy Bay, Tas 7005, Australia. [Nichols, David S.] Univ Tasmania, Cent Sci Lab, Sandy Bay, Tas 7005, Australia. [Smith, Jason] Univ Tasmania, Sch Chem, Sandy Bay, Tas 7005, Australia. [Chourey, Prem S.] USDA ARS, Gainesville, FL 32608 USA. RP Ross, JJ (reprint author), Univ Tasmania, Sch Biol Sci, Sandy Bay, Tas 7005, Australia. EM john.ross@utas.edu.au FU Australian Research Council FX We thank Noel Davies for his UPLC-MS expertise, Michelle Lang and Tracey Winterbottom for their assistance with glasshouse operations, and the Australian Research Council for financial support. NR 60 TC 2 Z9 2 U1 3 U2 6 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD JUN PY 2016 VL 171 IS 2 BP 1230 EP 1241 DI 10.1104/pp.16.00454 PG 12 WC Plant Sciences SC Plant Sciences GA DS3RA UT WOS:000380699200036 PM 27208245 ER PT J AU Sun, DD She, JY Gower, JL Stokes, CE Windham, GL Baird, RE Mlsna, TE AF Sun, Dongdi She, Jinyan Gower, Julie L. Stokes, C. Elizabeth Windham, Gary L. Baird, Richard E. Mlsna, Todd E. TI Effects of Growth Parameters on the Analysis of Aspergillus flavus Volatile Metabolites SO SEPARATIONS LA English DT Article DE Aspergillus flavus; microbial volatile organic compound (MVOC); solid phase microextraction (SPME); principal component analysis (PCA); partial least square analysis (PLS) ID SOLID-PHASE MICROEXTRACTION; SPME-GC-MS; GAS CHROMATOGRAPHY/MASS SPECTROMETRY; ORGANIC-COMPOUNDS; AFLATOXIN PRODUCTION; INDOOR MOLDS; IN-VITRO; MASS-SPECTROMETRY; ELECTRONIC NOSE; FUNGAL GROWTH AB Aspergillus flavus produces dangerous secondary metabolites known as aflatoxins, which are toxic and carcinogenic, and their contamination of agricultural products results in health issues and economic hardships in the U.S. and around the world. Early identification of aflatoxigenic isolates of A. flavus is the key in the management of these fungi. An emerging detection method for specific fungi identification involves the analysis of microbial volatile organic compounds (MVOCs) released by the fungi. Complicating this approach is the understanding that many factors influence metabolic production, including growth parameters, such as growth media, temperature, spore counts and oxidation stress. In addition, analytical and data analysis methods can also influence the results. Several growth and analysis methods were evaluated and optimized in order to better understand the effect of the methods on fungi MVOC signatures. The results indicate that carboxen/polydimethylsiloxane (CAR/PDMS) has the best extraction efficiency for the MVOCs emitted by A. flavus. Both chemical defined agar (CDA) and chemical defined liquid (CDL) are suitable growth media for MVOC emission studies. The highest MVOC production was found at 30 degrees C. Log transformation was considered one of the best data pretreatment methods when analyzing MVOC data and resulted in the best principal component analysis (PCA) clustering in the experiments with different growth media. This study aims to elucidate fungal volatile organic compounds (VOCs) differences due to variations in growth parameters as a first step in the development of an analytical method for the monitoring of aflatoxigenic A. flavus contamination in crop storage facilities. C1 [Sun, Dongdi; She, Jinyan; Gower, Julie L.; Mlsna, Todd E.] Mississippi State Univ, Dept Chem, Starkville, MS 39762 USA. [Stokes, C. Elizabeth; Baird, Richard E.] Mississippi State Univ, Dept Biochem Mol Biochem Entomol & Plant Pathol, Starkville, MS 39762 USA. [Windham, Gary L.] ARS, USDA, Starkville, MS 39762 USA. RP Mlsna, TE (reprint author), Mississippi State Univ, Dept Chem, Starkville, MS 39762 USA. EM dongdi_sun1@baxter.com; js2786@msstate.edu; jlg531@msstate.edu; ces8@msstate.edu; gary.windham@ars.usda.gov; rbaird@plantpath.msstate.edu; tmlsna@chemistry.msstate.edu NR 63 TC 0 Z9 0 U1 9 U2 10 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2297-8739 EI 2227-9075 J9 SEPARATIONS JI Separations PD JUN PY 2016 VL 3 IS 2 AR 13 DI 10.3390/separations3020013 PG 20 WC Chemistry, Analytical SC Chemistry GA DS0AK UT WOS:000380257300003 ER PT J AU Wang, XD AF Wang, X. -D. TI FATTY LIVER, HEPATOCELLULAR CARCINOMA, AND TOMATO LYCOPENE INTERVENTION SO ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH LA English DT Meeting Abstract CT 39th Annual Scientific Meeting of the Research-Society-on-Alcoholism (RSA) CY JUN 25-29, 2016 CL New Orleans, LA SP Res Soc Alcoholism C1 [Wang, X. -D.] Tufts Univ, Nutr & Canc Biol Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0145-6008 EI 1530-0277 J9 ALCOHOL CLIN EXP RES JI Alcoholism (NY) PD JUN PY 2016 VL 40 SU 1 SI SI MA 002 BP 248A EP 248A PG 1 WC Substance Abuse SC Substance Abuse GA DR3PH UT WOS:000379814602002 ER PT J AU Penaluna, BE Abadia-Cardoso, A Dunham, JB Garcia-De Leon, FJ Gresswell, RE Luna, AR Taylor, EB Shepard, BB Al-Chokhachy, R Muhlfeld, CC Bestgen, KR Rogers, K Escalante, MA Keeley, ER Temple, GM Williams, JE Matthews, KR Pierce, R Mayden, RL Kovach, RP Garza, JC Fausch, KD AF Penaluna, Brooke E. Abadia-Cardoso, Alicia Dunham, Jason B. Garcia-De Leon, Francisco J. Gresswell, Robert E. Ruiz Luna, Arturo Taylor, Eric B. Shepard, Bradley B. Al-Chokhachy, Robert Muhlfeld, Clint C. Bestgen, Kevin R. Rogers, Kevin Escalante, Marco A. Keeley, Ernest R. Temple, Gabriel M. Williams, Jack E. Matthews, Kathleen R. Pierce, Ron Mayden, Richard L. Kovach, Ryan P. Garza, John Carlos Fausch, Kurt D. TI Conservation of Native Pacific Trout Diversity in Western North America SO FISHERIES LA English DT Article ID WESTSLOPE CUTTHROAT TROUT; NONNATIVE BROOK TROUT; ONCORHYNCHUS-CLARKII-LEWISI; CLIMATE-CHANGE; RAINBOW-TROUT; UNITED-STATES; REDBAND TROUT; RIVER-BASIN; WATER FISH; WILD TROUT AB Pacific trout Oncorhynchus spp. in western North America are strongly valued in ecological, socioeconomic, and cultural views, and have been the subject of substantial research and conservation efforts. Despite this, the understanding of their evolutionary histories, overall diversity, and challenges to their conservation is incomplete. We review the state of knowledge on these important issues, focusing on Pacific trout in the genus Oncorhynchus. Although most research on salmonid fishes emphasizes Pacific salmon, we focus on Pacific trout because they share a common evolutionary history, and many taxa in western North America have not been formally described, particularly in the southern extent of their ranges. Research in recent decades has led to the revision of many hypotheses concerning the origin and diversification of Pacific trout throughout their range. Although there has been significant success at addressing past threats to Pacific trout, contemporary and future threats represented by nonnative species, land and water use activities, and climate change pose challenges and uncertainties. Ultimately, conservation of Pacific trout depends on how well these issues are understood and addressed, and on solutions that allow these species to coexist with a growing scope of human influences. C1 [Penaluna, Brooke E.] US Forest Serv, Pacific Northwest Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. [Abadia-Cardoso, Alicia] Univ Autonoma Baja California, Fac Ciencias Marinas, Ensenada, Baja California, Mexico. [Abadia-Cardoso, Alicia; Garza, John Carlos] Univ Calif Santa Cruz, Inst Marine Sci, Santa Cruz, CA 95064 USA. [Dunham, Jason B.] US Geol Survey, Forest Rangeland Ecosyst Sci Ctr, Corvallis Res Grp, Corvallis, OR USA. [Garcia-De Leon, Francisco J.] Ctr Invest Biol Noroeste SC, Lab Genet Conservac, Playa Palo de Santa Rita Sur, La Paz, Mexico. [Gresswell, Robert E.; Al-Chokhachy, Robert] US Geol Survey, Northern Rocky Mt Sci Ctr, Bozeman, MT USA. [Ruiz Luna, Arturo; Escalante, Marco A.] Ctr Invest Alimentac & Desarrollo, Unidad Mazatlan Acuicultura & Manejo Ambiental, Mazatlan, Sin, Mexico. [Taylor, Eric B.] Univ British Columbia, Biodivers Res Ctr, Dept Zool, Vancouver, BC, Canada. [Taylor, Eric B.] Univ British Columbia, Beaty Biodivers Museum, Vancouver, BC, Canada. [Shepard, Bradley B.] Wildlife Conservat Soc, Bozeman, MT USA. [Muhlfeld, Clint C.; Kovach, Ryan P.] US Geol Survey, Northern Rocky Mt Sci Ctr, Glacier Field Stn, Glacier Natl Pk, West Glacier, MT USA. [Muhlfeld, Clint C.] Univ Montana, Flathead Lake Biol Stn, Polson, MT 59860 USA. [Bestgen, Kevin R.] Colorado State Univ, Larval Fish Lab, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Rogers, Kevin] Colorado Pk & Wildlife, Steamboat Springs, CO USA. [Escalante, Marco A.] CNRS, Montpellier, France. [Keeley, Ernest R.] Idaho State Univ, Dept Biol Sci, Pocatello, ID 83209 USA. [Temple, Gabriel M.] Washington Dept Fish & Wildlife, Ecol Interact Team, Ellensburg, WA USA. [Williams, Jack E.] Trout Unltd, Medford, OR USA. [Matthews, Kathleen R.] US Forest Serv, Pacific Southwest Res Stn, Albany, CA USA. [Pierce, Ron] Montana Fish Wildlife & Pk, Missoula, MT USA. [Mayden, Richard L.] St Louis Univ, Dept Biol, St Louis, MO 63103 USA. [Garza, John Carlos] Natl Marine Fisheries Serv, Southwest Fisheries Sci Ctr, Fisheries Ecol Div, Santa Cruz, CA USA. [Fausch, Kurt D.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Shepard, Bradley B.] BB Shepard & Associates, Livingston, MT USA. RP Penaluna, BE (reprint author), US Forest Serv, Pacific Northwest Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM bepenaluna@fs.fed.us FU CONACYT [CB-2010-01-152893]; CONABIO [JM058]; U.S. Forest Service, PNW Research Station FX CONACYT (Ref. CB-2010-01-152893) and CONABIO (Ref. JM058) supported research projects on niche modeling and landscape genetics of native trout in Mexico. The U.S. Forest Service, PNW Research Station funded the use of illustrations by Joseph R. Tomelleri. This article has been peer reviewed and approved for publication consistent with U.S. Geological Survey Fundamental Science Practices (pubs. usgs. gov. circ/1367). Use of trade or firm names here is for reader information only and does not constitute endorsement of any product or service by the U.S. Government. NR 165 TC 0 Z9 0 U1 16 U2 18 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0363-2415 EI 1548-8446 J9 FISHERIES JI Fisheries PD JUN PY 2016 VL 41 IS 6 BP 286 EP 300 DI 10.1080/03632415.2016.1175888 PG 15 WC Fisheries SC Fisheries GA DR8KQ UT WOS:000380147200007 ER PT J AU Thomson, JL Zoellner, JM Tussing-Humphreys, LM Goodman, MH AF Thomson, J. L. Zoellner, J. M. Tussing-Humphreys, L. M. Goodman, M. H. TI Moderators of intervention dose effects on diet quality and physical activity changes in a church-based, multicomponent, lifestyle study: Delta Body and Soul III SO HEALTH EDUCATION RESEARCH LA English DT Article ID AFRICAN-AMERICAN WOMEN; NUTRITION KNOWLEDGE; MISSISSIPPI DELTA; HEALTH-PROMOTION AB Many community-based lifestyle interventions targeting African Americans have reported positive effects on participants' dietary choices and physical activity habits. However, these effects vary and not all participants will have outcome changes. Moderation analysis can help explain differential effects observed, but are not often reported. Hence, the objective of this secondary analysis was to explore potential moderators of intervention dose effects on diet quality and physical activity outcomes in an effective lifestyle intervention. Delta Body and Soul III, conducted from 2011 to 2012, was a 6-month, church-based, multicomponent, educational intervention designed to improve diet quality and increase physical activity in rural Southern African American adults. Generalized linear mixed models were used to determine associations among indicators of intervention dose received by participants, potential moderators and health outcome changes. Results indicated only three baseline characteristics-employment status, food shopping frequency and individual with primary responsibility for meal preparation-moderated the effects of education session attendance on diet quality changes. No evidence for moderation of exercise class attendance effects on physical activity changes was found. Thus, this culturally targeted, multicomponent lifestyle intervention did induce positive health changes in participants with a range of sociodemographic characteristics and food shopping and eating behaviors. C1 [Thomson, J. L.; Tussing-Humphreys, L. M.; Goodman, M. H.] ARS, USDA, Stoneville, MS 38776 USA. [Zoellner, J. M.] Virginia Tech, Dept Human Nutr Foods & Exercise, Blacksburg, VA 24060 USA. [Tussing-Humphreys, L. M.] Univ Illinois, Dept Med, Chicago, IL 60618 USA. [Tussing-Humphreys, L. M.] Univ Illinois, Ctr Canc, Chicago, IL 60618 USA. RP Thomson, JL (reprint author), ARS, USDA, Stoneville, MS 38776 USA. EM jessica.thomson@ars.usda.gov FU US Department of Agriculture, Agricultural Research Service [6401-51000-001-00D]; US Department of Health and Human Services, Health Resources Services Administration [6 U1FRHA07411] FX This work was supported by the US Department of Agriculture, Agricultural Research Service (project 6401-51000-001-00D); and the US Department of Health and Human Services, Health Resources Services Administration (6 U1FRHA07411). The views expressed are solely those of the authors' and do not reflect the official policy or position of the US government. NR 26 TC 0 Z9 0 U1 3 U2 3 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0268-1153 EI 1465-3648 J9 HEALTH EDUC RES JI Health Educ. Res. PD JUN PY 2016 VL 31 IS 3 BP 339 EP 349 DI 10.1093/her/cyw008 PG 11 WC Education & Educational Research; Public, Environmental & Occupational Health SC Education & Educational Research; Public, Environmental & Occupational Health GA DR9WQ UT WOS:000380247200004 PM 26944868 ER PT J AU Pureswaran, DS Hofstetter, RW Sullivan, BT Grady, AM Brownie, C AF Pureswaran, Deepa S. Hofstetter, Richard W. Sullivan, Brian T. Grady, Amanda M. Brownie, Cavell TI Western Pine Beetle Populations in Arizona and California Differ in the Composition of Their Aggregation Pheromones (vol 42, pg 404, 2016) SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Correction C1 [Pureswaran, Deepa S.] Canadian Forest Serv, Laurentian Forestry Ctr, 1055 Rue PEPS, Quebec City, PQ G1V 4C7, Canada. [Hofstetter, Richard W.] No Arizona Univ, Sch Forestry, Box 15018, Flagstaff, AZ 86011 USA. [Sullivan, Brian T.] US Forest Serv, USDA, Southern Res Stn, Pineville, LA 71360 USA. [Grady, Amanda M.] US Forest Serv, USDA, Forest Hlth Protect, Flagstaff, AZ 86001 USA. [Brownie, Cavell] North Carolina State Univ, Dept Stat, Raleigh, NC 27695 USA. RP Pureswaran, DS (reprint author), Canadian Forest Serv, Laurentian Forestry Ctr, 1055 Rue PEPS, Quebec City, PQ G1V 4C7, Canada. EM deepa.pureswaran@canada.ca NR 1 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 EI 1573-1561 J9 J CHEM ECOL JI J. Chem. Ecol. PD JUN PY 2016 VL 42 IS 6 BP 552 EP 552 DI 10.1007/s10886-016-0710-2 PG 1 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA DR7ET UT WOS:000380063500011 ER PT J AU Li, YX Li, CH Bradbury, PJ Liu, XL Lu, F Romay, CM Glaubitz, JC Wu, X Peng, B Shi, YS Song, YC Zhang, DF Buckler, ES Zhang, ZW Li, Y Wang, TY AF Li, Yong-xiang Li, Chunhui Bradbury, Peter J. Liu, Xiaolei Lu, Fei Romay, Cinta M. Glaubitz, Jeffrey C. Wu, Xun Peng, Bo Shi, Yunsu Song, Yanchun Zhang, Dengfeng Buckler, Edward S. Zhang, Zhiwu Li, Yu Wang, Tianyu TI Identification of genetic variants associated with maize flowering time using an extremely large multi-genetic background population SO PLANT JOURNAL LA English DT Article DE maize (Zea mays L.); flowering time; genome-wide association study (GWAS); linkage analysis; nested association mapping (NAM) ID GENOME-WIDE ASSOCIATION; LOCUS-T; POSTDOMESTICATION SPREAD; PHOTOPERIOD SENSITIVITY; ARABIDOPSIS; PATHWAYS; RICE; ARCHITECTURE; CONSTANS; COMPLEX AB Flowering time is one of the major adaptive traits in domestication of maize and an important selection criterion in breeding. To detect more maize flowering time variants we evaluated flowering time traits using an extremely large multi-genetic background population that contained more than 8000 lines under multiple Sino-United States environments. The population included two nested association mapping (NAM) panels and a natural association panel. Nearly 1 million single -nucleotide polymorphisms (SNPs) were used in the analyses. Through the parallel linkage analysis of the two NAM panels, both common and unique flowering time regions were detected. Genome wide, a total of 90 flowering time regions were identified. One-third of these regions were connected to traits associated with the environmental sensitivity of maize flowering time. The genome-wide association study of the three panels identified nearly 1000 flowering time -associated SNPs, mainly distributed around 220 candidate genes (within a distance of 1 Mb). Interestingly, two types of regions were significantly enriched for these associated SNPs - one was the candidate gene regions and the other was the approximately 5 kb regions away from the candidate genes. Moreover, the associated SNPs exhibited high accuracy for predicting flowering time. C1 [Li, Yong-xiang; Li, Chunhui; Wu, Xun; Peng, Bo; Shi, Yunsu; Song, Yanchun; Zhang, Dengfeng; Li, Yu; Wang, Tianyu] Chinese Acad Agr Sci, Inst Crop Sci, Beijing 10008, Peoples R China. [Bradbury, Peter J.; Liu, Xiaolei; Lu, Fei; Romay, Cinta M.; Glaubitz, Jeffrey C.; Buckler, Edward S.] Cornell Univ, Inst Genom Div, Ithaca, NY 14853 USA. [Buckler, Edward S.] USDA ARS, Ithaca, NY 14853 USA. [Zhang, Zhiwu] Northeast Agr Univ, Dept Anim Sci, Harbin 150030, Heilongjiang, Peoples R China. [Zhang, Zhiwu] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. RP Li, Y; Wang, TY (reprint author), Chinese Acad Agr Sci, Inst Crop Sci, Beijing 10008, Peoples R China.; Buckler, ES (reprint author), Cornell Univ, Inst Genom Div, Ithaca, NY 14853 USA.; Buckler, ES (reprint author), USDA ARS, Ithaca, NY 14853 USA.; Zhang, ZW (reprint author), Northeast Agr Univ, Dept Anim Sci, Harbin 150030, Heilongjiang, Peoples R China.; Zhang, ZW (reprint author), Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. EM esb33@cornell.edu; zhiwu.zhang@wsu.edu; liyu03@caas.cn; wangtianyu@caas.cn FU Ministry of Science and Technology of China [2011DFA30450, 2014BAD01B00, 2011CB100100, 2014DFG31690]; NSF of China [91335206, U1138304]; 'Innovation Program' of CAAS; NSF [0820619, 1238014]; USDA-ARS of the United States of America; Agricultural Research Center at Washington State University; Washington Grain Commission [126593] FX We thank Junjie Fu for sharing information about the maize gene list and annotations, Dallas Kroon for assisting with phenotype data management and Alex Lipka and Huihui Li for assisting with data analysis. This work was supported by the Ministry of Science and Technology of China (2011DFA30450, 2014BAD01B00, 2011CB100100, 2014DFG31690), NSF of China (91335206, U1138304), the 'Innovation Program' of CAAS, NSF #0820619 and #1238014, and the USDA-ARS of the United States of America, an Emerging Research Issues Internal Competitive Grant from the Agricultural Research Center at Washington State University, the Endowment and Research Project (no. 126593) from the Washington Grain Commission. We also thank Linda R. Klein for valuable advice and editing the manuscript. NR 57 TC 3 Z9 3 U1 13 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0960-7412 EI 1365-313X J9 PLANT J JI Plant J. PD JUN PY 2016 VL 86 IS 5 BP 391 EP 402 DI 10.1111/tpj.13174 PG 12 WC Plant Sciences SC Plant Sciences GA DR8SM UT WOS:000380167600003 PM 27012534 ER PT J AU Yin, XR Xie, XL Xia, XJ Yu, JQ Ferguson, IB Giovannoni, JJ Chen, KS AF Yin, Xue-ren Xie, Xiu-lan Xia, Xiao-jian Yu, Jing-quan Ferguson, Ian B. Giovannoni, James J. Chen, Kun-song TI Involvement of an ethylene response factor in chlorophyll degradation during citrus fruit degreening SO PLANT JOURNAL LA English DT Article DE chlorophyll degradation; Citrus sinensis; fruit degreening; CitERF13; ethylene response factor ID NAC TRANSCRIPTION FACTOR; LEAF SENESCENCE; ARABIDOPSIS-THALIANA; ABSCISIC-ACID; BREAKDOWN; BIOSYNTHESIS; LOCALIZATION; ACCUMULATION; EXPRESSION; INTERACT AB Chlorophyll degradation naturally occurs during plant senescence. However, in fruit such as citrus, it is a positive characteristic, as degreening is an important colour development contributing to fruit quality. In the present work, Citrus sinensis Osbeck, cv. Newhall fruit was used as a model for chlorophyll degradation. An ethylene response factor, CitERF13, was isolated and its transcriptional changes were closely correlated with fruit peel degreening during development or in response to ethylene. Dual-luciferase and yeast one hybrid assays, as well as motif mutation, indicated that CitERF13 directly binds to the CitPPH promoter and enhances its activity. Transient and stable over-expression of CitERF13 resulted in rapid chlorophyll degradation in Nicotiana tabacum leaves and led to accumulation of pheophorbide (Pheide) a, a metabolite of pheophorbide hydrolase (PPH). Similar results were observed from transient transformation of CitERF13 in citrus fruit peel. Moreover, this function of CitERF13 was conserved within Arabidopsis and tomato, as the homologs AtERF17 and SIERF16 similarly acted as activators of PPH genes and accelerators of chlorophyll degradation. C1 [Yin, Xue-ren; Xie, Xiu-lan; Xia, Xiao-jian; Yu, Jing-quan; Ferguson, Ian B.; Chen, Kun-song] Zhejiang Univ, Coll Agr & Biotechnol, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China. [Yin, Xue-ren; Xia, Xiao-jian; Yu, Jing-quan; Chen, Kun-song] Zhejiang Univ, Zhejiang Prov Key Lab Hort Plant Integrat Biol, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China. [Yin, Xue-ren; Xia, Xiao-jian; Yu, Jing-quan; Chen, Kun-song] Zhejiang Univ, State Agr Minist, Lab Hort Plant Growth Dev & Qual Improvement, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China. [Ferguson, Ian B.] New Zealand Inst Plant & Food Res Ltd, Private Bag 92169, Auckland, New Zealand. [Giovannoni, James J.] Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Giovannoni, James J.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Chen, KS (reprint author), Zhejiang Univ, Coll Agr & Biotechnol, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China.; Chen, KS (reprint author), Zhejiang Univ, Zhejiang Prov Key Lab Hort Plant Integrat Biol, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China.; Chen, KS (reprint author), Zhejiang Univ, State Agr Minist, Lab Hort Plant Growth Dev & Qual Improvement, Zijingang Campus, Hangzhou 310058, Zhejiang, Peoples R China. EM akun@zju.edu.cn RI Yin, Xue-ren/B-3893-2008 OI Yin, Xue-ren/0000-0002-1282-4432 FU Program of International Science sand Technology Cooperation [2011DFB31580]; National Basic Research Program of China [2011CB100602] FX Authors would like to thank Miss Qian Jiang (Zhejiang University) for collection of developmental orange fruit. This research was supported by the Program of International Science sand Technology Cooperation (2011DFB31580), and the National Basic Research Program of China (2011CB100602). The authors have no conflict of interest to declare. NR 40 TC 2 Z9 3 U1 28 U2 35 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0960-7412 EI 1365-313X J9 PLANT J JI Plant J. PD JUN PY 2016 VL 86 IS 5 BP 403 EP 412 DI 10.1111/tpj.13178 PG 10 WC Plant Sciences SC Plant Sciences GA DR8SM UT WOS:000380167600004 PM 27037684 ER PT J AU Martinez-Flores, F Arbizu, CI Reitsma, K Juan, A Simon, PW Spooner, DM Crespo, MB AF Martinez-Flores, Fernando Arbizu, Carlos I. Reitsma, Kathleen Juan, Ana Simon, Philipp W. Spooner, David M. Crespo, Manuel B. TI Lectotype Designation for Seven Species Names in the Daucus guttatus Complex (Apiaceae) from the Central and Eastern Mediterranean Basin SO SYSTEMATIC BOTANY LA English DT Article DE Carrot species; Mediterranean flora; nomenclature; typification ID MOLECULAR PHYLOGENY; DISJUNCTIONS AB The Daucus guttatus complex includes two to four species growing from central and northern Italy to the Middle East. They are characterized by being typically annuals up to 50 cm high; with primary umbels up to 7 cm in diameter with fewer than 25( 35) rays; discolored umbels frequent, bearing one to several dark colored umbellules which form different color patterns; and mericarps relatively small, ca. 2.0-4.5 mm. The taxonomy of this complicated group has not been satisfactorily resolved to date and is the focus of current research. Seven names of species belonging to the D. guttatus complex occurring in the central and eastern Mediterranean basin are typified here: Daucus guttatus, Daucus bicolor, Daucus involucratus, Daucus setulosus, Daucus broteri, Daucus hirsutus, and Daucus speciosus. Historical data are reported to justify lectotype and/or epitype selection, and selected morphological and distributional data are used to facilitate identification. The resulting typifications will enable proper naming of clades identified in the accompanying integrated molecular and morphological study, clarifying the taxonomy of the Daucus guttatus complex. C1 [Martinez-Flores, Fernando; Juan, Ana; Crespo, Manuel B.] Univ Alicante, Dept Ciencias Ambientales & Recursos Nat, POB 99, ES-03080 Alicante, Spain. [Martinez-Flores, Fernando; Juan, Ana; Crespo, Manuel B.] Univ Alicante, Ctr Iberoamer Biodiversidad, POB 99, ES-03080 Alicante, Spain. [Arbizu, Carlos I.; Simon, Philipp W.; Spooner, David M.] ARS, USDA, Vegetable Crops Res Unit, 1575 Linden Dr, Madison, WI 53706 USA. [Arbizu, Carlos I.; Simon, Philipp W.; Spooner, David M.] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Reitsma, Kathleen] Iowa State Univ, North Cent Reg Plant Introduct Stn, Ames, IA 50011 USA. RP Martinez-Flores, F (reprint author), Univ Alicante, Dept Ciencias Ambientales & Recursos Nat, POB 99, ES-03080 Alicante, Spain.; Martinez-Flores, F (reprint author), Univ Alicante, Ctr Iberoamer Biodiversidad, POB 99, ES-03080 Alicante, Spain. EM f.martinez@ua.es NR 39 TC 3 Z9 3 U1 1 U2 1 PU AMER SOC PLANT TAXONOMISTS PI LARAMIE PA UNIV WYOMING, DEPT BOTANY 3165, 1000 E UNIVERSITY AVE, LARAMIE, WY 82071 USA SN 0363-6445 EI 1548-2324 J9 SYST BOT JI Syst. Bot. PD JUN PY 2016 VL 41 IS 2 BP 464 EP 478 DI 10.1600/036364416X691849 PG 15 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA DR9UE UT WOS:000380240800018 ER PT J AU Arbizu, CI Simon, PW Martinez-Flores, F Ruess, H Crespo, MB Spooner, DM AF Arbizu, Carlos I. Simon, Philipp W. Martinez-Flores, Fernando Ruess, Holly Crespo, Manuel B. Spooner, David M. TI Integrated Molecular and Morphological Studies of the Daucus guttatus Complex (Apiaceae) SO SYSTEMATIC BOTANY LA English DT Article DE Carrot species; germplasm; nuclear orthologs; phenetics ID NUCLEAR RIBOSOMAL DNA; DARK CENTRAL FLORET; PHYLOGENETIC ANALYSIS; SUBFAMILY APIOIDEAE; CAROTA L.; EVOLUTION; FRUIT; SEQUENCES; DISCRIMINATION; DISJUNCTIONS AB In a previous study using 94 nuclear orthologs, we reported the species status of the Daucus guttatus complex to be unresolved, partitioned into three clades. In the present study, a subset of ten of these 94 orthologs was used to infer the phylogeny of the D. guttatus complex and related species. A near parallel set of accessions, planted in a common garden, was used for morphological analyses. The molecular trees are highly resolved for most of the clades, grouping accessions of the D. guttatus complex into four clades. Bayesian concordance analysis and a coalescent approach gave slightly different topologies. Morphological data likewise support four taxa in the complex. Moreover, herbarium research from a companion study informs nomenclature for taxa of the complex. We identify these four clades as D. bicolor, D. conchitae, D. guttatus, and D. setulosus; internested in or among these segregates are the phenetically distinctive species D. glochidiatus, D. involucratus, D. littoralis, and D. pusillus. Our research redefines species variation in the D. guttatus complex, clarifies species names, interspecific relationships, confirms a useful subset of nuclear orthologs for studies of dominant topologies of Daucus, and discovers morphological characters allowing proper identification of the four species of the D. guttatus complex and related species. C1 [Arbizu, Carlos I.; Simon, Philipp W.; Ruess, Holly; Spooner, David M.] Univ Wisconsin, USDA, ARS, Vegetable Crops Res Unit,Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Martinez-Flores, Fernando; Crespo, Manuel B.] Univ Alicante, Dept Ciencias Ambientales & Recursos Nat, POB 99, ES-03080 Alicante, Spain. [Martinez-Flores, Fernando; Crespo, Manuel B.] Univ Alicante, Ctr Iberoamer Biodiversidad CIBIO, POB 99, ES-03080 Alicante, Spain. RP Spooner, DM (reprint author), Univ Wisconsin, USDA, ARS, Vegetable Crops Res Unit,Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. EM David.Spooner@ars.usda.gov FU National Council of Science and Technology of Peru (Concytec) FX We thank Kathleen Reitsma at the North Central Regional Plant Introduction Station in Ames, Iowa for providing germplasm. This paper represents partial fulfillment of a Ph.D. degree for CA in Plant Breeding and Plant Genetics at the University of Wisconsin-Madison. CA is partly funded by the National Council of Science and Technology of Peru (Concytec, by its initials in Spanish). We appreciate the comments of two anonymous reviewers and Editor-in Chief James Smith that greatly improved our manuscript. NR 94 TC 3 Z9 3 U1 2 U2 2 PU AMER SOC PLANT TAXONOMISTS PI LARAMIE PA UNIV WYOMING, DEPT BOTANY 3165, 1000 E UNIVERSITY AVE, LARAMIE, WY 82071 USA SN 0363-6445 EI 1548-2324 J9 SYST BOT JI Syst. Bot. PD JUN PY 2016 VL 41 IS 2 BP 479 EP 492 DI 10.1600/036364416X691948 PG 14 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA DR9UE UT WOS:000380240800019 ER PT J AU Wang, QQ Zhu, JY AF Wang, Qianqian Zhu, J. Y. TI Effects of mechanical fibrillation time by disk grinding on the properties of cellulose nanofibrils SO TAPPI JOURNAL LA English DT Article ID TRANSPARENT; NANOCOMPOSITES; NANOCRYSTALS; FIBERS; PULP; CNC AB Cellulose nanofibrils (CNF) were successfully produced from a bleach kraft eucalyptus pulp by a supermasscolloider. Effects of grinding time on structure and properties of CNF and the corresponding CNF films were investigated. Grinding time was important to increase the optical transparency of CNF suspensions. The degree of polymerization (DP) and crystallinity index (CrI) of CNF decreased linearly with the increase in CNF suspension transparency. This suggests optical transparency of a CNF suspension can be used to characterize the degree of fibrillation. Specific tensile strength and Young's modulus of the CNF films made of CNF suspension with only 0.5 h grinding were increased approximately 30% and 200%, respectively, compared with conventional handsheets prepared by valley beating to 300 Canadian Standard Freeness (CSF). Energy input was only 1.38 kWh/kg for 0.5 h grinding. Grinding beyond 0.5 h produced negligible improvement in specific tensile and specific modulus. Opacity of CNF films decreased rapidly during the first 1.5 h of fibrillation and then plateaued. Application: Disk milling time affects the morphology of cellulose nanofibrils as well as the optical and mechanical properties of film made of the resultant fibrils. C1 [Wang, Qianqian] Jiangsu Univ, Sch Environm, Biofuels Inst, Zhenjiang, Peoples R China. [Zhu, J. Y.] USDA Forest Prod Lab, Madison, WI 53726 USA. RP Zhu, JY (reprint author), USDA Forest Prod Lab, Madison, WI 53726 USA. EM jzhu@fs.fed.us FU USDA Agriculture and Food Research Initiative (AFRI) Competitive Grant [2011-67009-20056]; National Natural Science Foundation of China [31300493]; Jiangsu University FX This work was funded by the USDA Agriculture and Food Research Initiative (AFRI) Competitive Grant No. 2011-67009-20056 and the National Natural Science Foundation of China No. 31300493, Young Scholar Program sponsored by Jiangsu University. The authors acknowledge SAIC-Frederick, Inc., U.S. National Cancer Institute, for transmission electron microscopy imaging. NR 22 TC 0 Z9 0 U1 2 U2 2 PU TECH ASSOC PULP PAPER IND INC PI NORCROSS PA 15 TECHNOLOGY PARK SOUTH, NORCROSS, GA 30092 USA SN 0734-1415 J9 TAPPI J JI TAPPI J. PD JUN PY 2016 VL 15 IS 6 BP 419 EP 423 PG 5 WC Materials Science, Paper & Wood SC Materials Science GA DS0EJ UT WOS:000380267900008 ER PT J AU Shatkin, JA Ong, KJ Ede, JD Wegner, TH Goergen, M AF Shatkin, Jo Anne Ong, Kimberly J. Ede, James D. Wegner, Theodore H. Goergen, Michael TI Toward cellulose nanomaterial commercialization: Knowledge gap analysis for Safety Data Sheets according to the Globally Harmonized System SO TAPPI JOURNAL LA English DT Article ID PURIFIED CELLULOSE; DIETARY FIBER; IN-VITRO; NANOCRYSTALS; TOXICITY; NANOPARTICLES; ENVIRONMENT; RATS AB Commercialization of cellulose nanomaterials (CNs) is rapidly advancing, to the benefit of many end-use product sectors, and providing information about the safe manufacturing and handling for CNs is a priority. Safety Data Sheets (SDS) are required for industrially produced materials to communicate information on their potential health, fire, reactivity, and environmental hazards, and to provide recommendations on how to safely work with these materials. Cellulose and cellulose pulp, which have widespread commercial end uses, can create nuisance dusts when dried and are required to have SDS. We therefore expect that nanoscale forms of cellulose will also require SDS. This study identifies the currently available SDS information for CNs and highlights existing gaps in our knowledge. With U.S. and international adoption of the Globally Harmonized System (GHS) for Hazard Communication, producers are required to report SDS known data and data gaps. Given the novelty of all nanomaterials, it is preferable to fill these gaps in SDS as a demonstration of our commitment to the safe production and use of these materials. To evaluate the availability of SDS information and prepare for commercialization of CNs, we assessed available safety information for CNs to identify available GHS SDS data, data gaps, and what data need to yet be developed to fully classify CNs according to the GHS. Specifically, we report on the available data and gaps regarding the toxicological profile, environmental characteristics, physical and chemical properties, exposure controls, and personal protection for cellulose nanomaterials, to encourage the development of missing data and advance safe commercialization. Application: By providing information on available and missing data and research needed to communicate the safe handling and use of CNs according to the criteria set by the GHS for SDS, industry can demonstrate the safety of these materials, ensuring their maximum market potential is reached, and commercialization is not delayed because of uncertainties about safe use. C1 [Shatkin, Jo Anne; Ong, Kimberly J.; Ede, James D.] Vireo Advisors, Boston, MA 02130 USA. [Wegner, Theodore H.] US Forest Serv, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53705 USA. [Goergen, Michael] US Endowment Forestry & Communities, P3Nano, Greenville, SC USA. RP Shatkin, JA (reprint author), Vireo Advisors, Boston, MA 02130 USA. EM jashatkin@vireoadvisors.com NR 57 TC 0 Z9 0 U1 3 U2 3 PU TECH ASSOC PULP PAPER IND INC PI NORCROSS PA 15 TECHNOLOGY PARK SOUTH, NORCROSS, GA 30092 USA SN 0734-1415 J9 TAPPI J JI TAPPI J. PD JUN PY 2016 VL 15 IS 6 BP 425 EP 437 PG 13 WC Materials Science, Paper & Wood SC Materials Science GA DS0EJ UT WOS:000380267900009 ER PT J AU Brown, RL Williams, WP Windham, GL Menkir, A Chen, ZY AF Brown, Robert L. Williams, W. Paul Windham, Gary L. Menkir, Abebe Chen, Zhi-Yuan TI Evaluation of African-Bred Maize Germplasm Lines for Resistance to Aflatoxin Accumulation SO AGRONOMY-BASEL LA English DT Article DE aflatoxin; host resistance; breeding; field trials ID ASPERGILLUS-FLAVUS; EAR ROT; REGISTRATION; KERNELS; CONTAMINATION; GENOTYPES; INBREDS AB Aflatoxins, produced by the fungus Aspergillus flavus, contaminate maize grain and threaten human food and feed safety. Plant resistance is considered the best strategy for reducing aflatoxin accumulation. Six maize germplasm lines, TZAR101-TZAR106, were released by the International Institute of Tropical Agriculture-Southern Regional Research Center (IITA-SRRC) maize breeding collaboration for use in African National Programs and U.S. maize breeding programs. The present investigation was conducted to evaluate aflatoxin reduction by these lines in a U.S. environment. As germplasm lines, resistance was demonstrated by the lines tested in 2010 and 2014 trials. In 2010, TZAR106 was among the lines with the lowest toxin accumulation, and in 2014, along with TZAR102, supported low aflatoxin. When evaluated as single cross hybrids in 2012, 2013 and 2014, several crosses involving IITA-SRRC lines accumulated low toxin. In 2012, TZAR103 x HBA1 was one of 4 lines with the lowest concentration of aflatoxin. In 2014, five IITA-SRRC hybrids were among the lowest with TZAR102 x Va35 and TZAR102 x LH132 being the two lowest. Results demonstrate significant aflatoxin reduction by IITA-SRRC lines in a U.S. aflatoxin-conducive environment (at Mississippi State University). Further testing in different locations and environments is needed to further evaluate the potential usefulness of these germplasm lines. C1 [Brown, Robert L.] ARS, USDA, Food & Feed Safety Res Unit, Southern Reg Res Ctr, New Orleans, LA 70124 USA. [Williams, W. Paul; Windham, Gary L.] ARS, USDA, Corn Host Plant Resistance Res Unit, Mississippi State, MS 39762 USA. [Menkir, Abebe] Int Inst Trop Agr, Oyo Rd,PMB 5320, Ibadan, Nigeria. [Chen, Zhi-Yuan] LSU Agr Ctr, Dept Plant Pathol & Crop Physiol, Baton Rouge, LA 70803 USA. RP Brown, RL (reprint author), ARS, USDA, Food & Feed Safety Res Unit, Southern Reg Res Ctr, New Orleans, LA 70124 USA. EM robert.brown@ars.usda.gov; paul.williams@ars.usda.gov; gary.windham@ars.usda.gov; a.menkir@cgiar.org; zchen@agcenter.lsu.edu NR 20 TC 0 Z9 0 U1 1 U2 1 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2073-4395 J9 AGRONOMY-BASEL JI Agronomy-Basel PD JUN PY 2016 VL 6 IS 2 AR 24 DI 10.3390/agronomy6020024 PG 10 WC Agronomy SC Agriculture GA DR5ZL UT WOS:000379980800004 ER PT J AU Herrera, JM Rubio, G Haner, LL Delgado, JA Lucho-Constantino, CA Islas-Valdez, S Pellet, D AF Herrera, Juan M. Rubio, Gerardo Haner, Lilia Levy Delgado, Jorge A. Lucho-Constantino, Carlos A. Islas-Valdez, Samira Pellet, Didier TI Emerging and Established Technologies to Increase Nitrogen Use Efficiency of Cereals SO AGRONOMY-BASEL LA English DT Review DE nitrogen use efficiency; nanofertilizers; recycled fertilizers; slow N released fertilizers ID WINTER-WHEAT; LEAF NITROGEN; NITRIFICATION INHIBITORS; FERTILIZATION MANAGEMENT; BRADYRHIZOBIUM-JAPONICUM; PSEUDOMONAS-PUTIDA; GRAIN PROTEIN; OILSEED RAPE; PLANT-GROWTH; AZOSPIRILLUM-BRASILENSE AB Nitrogen (N) fertilizers are one of the most expensive inputs in agricultural settings. Additionally, the loss of N increases costs, contributes to soil acidification, and causes off-site pollution of the air, groundwater and waterways. This study reviews current knowledge about technologies for N fertilization with potential to increase N use efficiency and reduce its negative effects on the environment. Classic inorganic sources such as urea and ammonium sulfate are the major sources utilized, while controlled N release fertilizers have not been significantly adopted for cereals and oil crops. Microorganisms, with the exception of Rhizobium sp. in soybeans, are also not widely used nowadays (e.g., plant growth-promoting bacteria and cynobacteria). The interest in implementing new N fertilization knowledge is stimulating the development of sensors to diagnose the N status and decision support systems for integrating several variables to optimize sources, rates and methods of application. Among potential new technologies we identified the incipient development of nanofertilizers, nutrient formulations to coat seeds, and recycled nutrients. Furthermore, increasing concern about the environmental consequences of N may facilitate the implementation of innovations outside the farm such as more effective regulations to guide N fertilization and methods to manufacture N fertilizers that are more energy-efficient and less CO2 equivalent emitting. C1 [Herrera, Juan M.; Haner, Lilia Levy; Pellet, Didier] Agroscope, Route de Duillier 50, CH-1260 Nyon, Switzerland. [Rubio, Gerardo] Univ Buenos Aires, Fac Agron, Inst Invest Biociencias Agr & Ambientales INBA CO, C1417DSE, Buenos Aires, DF, Argentina. [Delgado, Jorge A.] USDA, Ft Collins, CO 80526 USA. [Lucho-Constantino, Carlos A.] Univ Autonoma Estado Hidalgo, Chem Res Ctr, Hidalgo 42076, Mexico. [Islas-Valdez, Samira] Univ Autonoma Madrid, Doctoral Programme Agr Chem, E-28049 Madrid, Spain. RP Herrera, JM (reprint author), Agroscope, Route de Duillier 50, CH-1260 Nyon, Switzerland. EM herreraj@agro.uba.ar; rubio@agro.uba.ar; lilia.levy@agroscope.admin.ch; Jorge.Delgado@ars.usda.gov; luchouaeh@gmail.com; slapiiv@hotmail.com; didier.pellet@agroscope.admin.ch NR 130 TC 1 Z9 1 U1 24 U2 37 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2073-4395 J9 AGRONOMY-BASEL JI Agronomy-Basel PD JUN PY 2016 VL 6 IS 2 AR 25 DI 10.3390/agronomy6020025 PG 19 WC Agronomy SC Agriculture GA DR5ZL UT WOS:000379980800005 ER PT J AU Pan, YB AF Pan, Yong-Bao TI Development and Integration of an SSR-Based Molecular Identity Database into Sugarcane Breeding Program SO AGRONOMY-BASEL LA English DT Editorial Material DE sugarcane breeding; SSR; molecular identity database ID REGISTRATION; MARKERS AB Sugarcane breeding is very difficult and it takes 12 to 14 years to develop a new cultivar for commercial production. This is because sugarcane varieties are highly polyploid, inter-specific hybrids with 100 to 130 chromosomes that may vary across geographical areas. Other obstacles/constraints include the small size of flowers that may not synchronize but may self-pollinate, difficulty in distinguishing hybrids from self progenies, extreme (G x E) interactive effect, and potential variety mis-identification during vegetative propagation and varietal exchange. To help cane breeders circumvent these constraints, a simple sequence repeats (SSR)-based molecular identity database has been developed at the United States Department of Agriculture-Agricultural Research Service, Sugarcane Research Unit in Houma, LA. Since 2005, approximately 2000 molecular identities have been constructed for clones of sugarcane and related Saccharum species that cover geographical areas including Argentina, Australia, Bangladesh, China, Colombia, India, Mexico, Pakistan, South Africa, Thailand, USA (Louisiana, Florida, Texas, and Hawaii), and Venezuela. The molecular identity database is updated annually and has been utilized to: (1) provide molecular descriptors to newly registered cultivars; (2) identify in a timely fashion any mislabeled or unidentifiable clones from cross parents and field evaluation plots; (3) develop de novo clones of energy cane with S. spontaneum cytoplasm; (4) provide clone-specific fingerprint information for assessing cross quality and paternity of polycross; (5) determine genetic relatedness of parental clones; (6) select F-1 hybrids from (elite x wild) or (wild x elite) crosses; and (7) investigate the inheritance of SSR markers in sugarcane. The integration of the molecular identity database into the sugarcane breeding program may improve the overall efficacy of cultivar development and commercialization. C1 [Pan, Yong-Bao] ARS, Southeast Area, Sugarcane Res Unit, USDA, 5883 USDA Rd, Houma, LA 70360 USA. RP Pan, YB (reprint author), ARS, Southeast Area, Sugarcane Res Unit, USDA, 5883 USDA Rd, Houma, LA 70360 USA. EM yongbao.pan@ars.usda.gov OI Pan, Yong-Bao/0000-0001-5133-1342 NR 21 TC 0 Z9 0 U1 0 U2 0 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2073-4395 J9 AGRONOMY-BASEL JI Agronomy-Basel PD JUN PY 2016 VL 6 IS 2 AR 28 DI 10.3390/agronomy6020028 PG 6 WC Agronomy SC Agriculture GA DR5ZL UT WOS:000379980800008 ER PT J AU Huang, M He, CJ Zhu, QB Qin, JW AF Huang, Min He, Chujie Zhu, Qibing Qin, Jianwei TI Maize Seed Variety Classification Using the Integration of Spectral and Image Features Combined with Feature Transformation Based on Hyperspectral Imaging SO APPLIED SCIENCES-BASEL LA English DT Article DE hyperspectral imaging; maize seed; classification; SPA; LS-SVM; feature transformation methods ID SUCCESSIVE PROJECTIONS ALGORITHM; NEAR-INFRARED SPECTRA; MUTUAL INFORMATION; NEURAL-NETWORK; IDENTIFICATION; RECOGNITION; SELECTION; KERNELS AB Hyperspectral imaging (HSI) technology has been extensively studied in the classification of seed variety. A novel procedure for the classification of maize seed varieties based on HSI was proposed in this study. The optimal wavelengths for the classification of maize seed varieties were selected using the successive projections algorithm (SPA) to improve the acquiring and processing speed of HSI. Subsequently, spectral and imaging features were extracted from regions of interest of the hyperspectral images. Principle component analysis and multidimensional scaling were then introduced to transform/reduce the classification features for overcoming the risk of dimension disaster caused by the use of a large number of features. Finally, the integrating features were used to develop a least squares-support vector machines (LS-SVM) model. The LS-SVM model, using the integration of spectral and image features combined with feature transformation methods, achieved more than 90% of test accuracy, which was better than the 83.68% obtained by model using the original spectral and image features, and much higher than the 76.18% obtained by the model only using the spectral features. This procedure provides a possible way to apply the multispectral imaging system to classify seed varieties with high accuracy. C1 [Huang, Min; He, Chujie; Zhu, Qibing] Jiangnan Univ, Key Lab Adv Proc Control Light Ind, Minist Educ, Wuxi 214122, Jiangsu, Peoples R China. [Qin, Jianwei] ARS, USDA, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Huang, M (reprint author), Jiangnan Univ, Key Lab Adv Proc Control Light Ind, Minist Educ, Wuxi 214122, Jiangsu, Peoples R China. EM huangmzqb@163.com; he_chujie@163.com; zhuqib@163.com; jianwei.qin@ars.usda.gov FU National Natural Science Foundation of China [61271384, 61275155]; 111 Project [B12018]; QingLan Project FX The authors gratefully acknowledge the financial support from the National Natural Science Foundation of China (Grant No. 61271384, 61275155), the 111 Project (B12018), and QingLan Project. NR 32 TC 0 Z9 0 U1 16 U2 21 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2076-3417 J9 APPL SCI-BASEL JI Appl. Sci.-Basel PD JUN PY 2016 VL 6 IS 6 AR 183 DI 10.3390/app6060183 PG 12 WC Chemistry, Multidisciplinary; Materials Science, Multidisciplinary; Physics, Applied SC Chemistry; Materials Science; Physics GA DR5FG UT WOS:000379928200025 ER PT J AU Lu, HS Crow, WT Zhu, YH Ouyang, F Su, JB AF Lu, Haishen Crow, Wade T. Zhu, Yonghua Ouyang, Fen Su, Jianbin TI Improving Streamflow Prediction Using Remotely-Sensed Soil Moisture and Snow Depth SO REMOTE SENSING LA English DT Article DE soil moisture; snow depth; precipitation; streamflow; data assimilation ID STATE-PARAMETER ESTIMATION; RAINFALL-RUNOFF MODEL; DATA ASSIMILATION; LAND-SURFACE; RETRIEVALS; SCATTEROMETER; TEMPERATURE; SIMULATION; CHINA; SCALE AB The monitoring of both cold and warm season hydrologic processes in headwater watersheds is critical for accurate water resource monitoring in many alpine regions. This work presents a new method that explores the simultaneous use of remotely sensed surface soil moisture (SM) and snow depth (SD) retrievals to improve hydrological modeling in such areas. In particular, remotely sensed SM and SD retrievals are applied to filter errors present in both solid and liquid phase precipitation accumulation products acquired from satellite remote sensing. Simultaneously, SM and SD retrievals are also used to correct antecedent SM and SD states within a hydrological model. In synthetic data assimilation experiments, results suggest that the simultaneous correction of both precipitation forcing and SM/SD antecedent conditions is more efficient at improving streamflow simulation than data assimilation techniques which focus solely on the constraint of antecedent SM or SD conditions. In a real assimilation case, results demonstrate the potential benefits of remotely sensed SM and SD retrievals for improving the representation of hydrological processes in a headwater basin. In particular, it is demonstrated that dual precipitation/state correction represents an efficient strategy for improving the simulation of cold-region hydrological processes. C1 [Lu, Haishen; Zhu, Yonghua; Ouyang, Fen; Su, Jianbin] Hohai Univ, Coll Hydrol & Water Resources, State Key Lab Hydrol Water Resources & Hydraul En, Nanjing 210098, Jiangsu, Peoples R China. [Lu, Haishen; Crow, Wade T.] USDA, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. RP Lu, HS (reprint author), Hohai Univ, Coll Hydrol & Water Resources, State Key Lab Hydrol Water Resources & Hydraul En, Nanjing 210098, Jiangsu, Peoples R China.; Lu, HS (reprint author), USDA, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. EM haishenlu@foxmail.com; Wade.Crow@ars.usda.gov; zhuyonghua@hhu.edu.cn; ouyangfennj@126.com; ncwu_hhu@126.com OI Lu, Haishen/0000-0002-1601-8892 FU National Basic Research Program of China [2013CBA01806]; NNSF [41371049, 41571015, 41323001, 51190091]; Fund of State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering [20145027312]; open fund of State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography FX This research was supported by National Basic Research Program of China (2013CBA01806), NNSF (41371049; 41571015; 41323001; 51190091), Fund of State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering (20145027312) and the open fund of State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography. NR 42 TC 0 Z9 0 U1 9 U2 12 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD JUN PY 2016 VL 8 IS 6 AR 503 DI 10.3390/rs8060503 PG 20 WC Remote Sensing SC Remote Sensing GA DR6BE UT WOS:000379985300062 ER PT J AU Bramwell, L AF Bramwell, Lincoln TI Federal Ecosystem Management: Its Rise, Fall, and Afterlife SO AGRICULTURAL HISTORY LA English DT Book Review C1 [Bramwell, Lincoln] US Forest Serv, USDA, Washington, DC 20250 USA. RP Bramwell, L (reprint author), US Forest Serv, USDA, Washington, DC 20250 USA. NR 1 TC 0 Z9 0 U1 2 U2 2 PU AGRICULTURAL HISTORY SOCIETY PI LITTLE ROCK PA UALR, DEPT HISTORY, 2801 S UNIVERSITY AVE, LITTLE ROCK, AR 72324-1099 USA SN 0002-1482 EI 1533-8290 J9 AGR HIST JI Agric. Hist. PD SUM PY 2016 VL 90 IS 3 BP 402 EP 404 PG 3 WC Agriculture, Multidisciplinary; History; History & Philosophy Of Science SC Agriculture; History; History & Philosophy of Science GA DR4PP UT WOS:000379885000008 ER PT J AU Kimball, BA Cohen, AS Gordon, AR Opiekun, M Martin, T Elkind, J Lundstrom, JN Beauchamp, GK AF Kimball, Bruce A. Cohen, Akiva S. Gordon, Amy R. Opiekun, Maryanne Martin, Talia Elkind, Jaclynn Lundstroem, Johan N. Beauchamp, Gary K. TI Brain Injury Alters Volatile Metabolome SO CHEMICAL SENSES LA English DT Article DE animal model; bioassay; chemical analysis; diagnosis; inflammation; traumatic brain injury; urine ID CHROMATOGRAPHY-MASS SPECTROMETRY; TUMOR-BEARING MICE; TRAUMATIC BRAIN; ORGANIC-COMPOUNDS; LUNG-CANCER; INDIVIDUAL RECOGNITION; COGNITIVE IMPAIRMENT; CEREBROSPINAL-FLUID; GUT MICROBIOTA; ODOR DETECTION AB Chemical signals arising from body secretions and excretions communicate information about health status as have been reported in a range of animal models of disease. A potential common pathway for diseases to alter chemical signals is via activation of immune function-which is known to be intimately involved in modulation of chemical signals in several species. Based on our prior findings that both immunization and inflammation alter volatile body odors, we hypothesized that injury accompanied by inflammation might correspondingly modify the volatile metabolome to create a signature endophenotype. In particular, we investigated alteration of the volatile metabolome as a result of traumatic brain injury. Here, we demonstrate that mice could be trained in a behavioral assay to discriminate mouse models subjected to lateral fluid percussion injury from appropriate surgical sham controls on the basis of volatile urinary metabolites. Chemical analyses of the urine samples similarly demonstrated that brain injury altered urine volatile profiles. Behavioral and chemical analyses further indicated that alteration of the volatile metabolome induced by brain injury and alteration resulting from lipopolysaccharide-associated inflammation were not synonymous. Monitoring of alterations in the volatile metabolome may be a useful tool for rapid brain trauma diagnosis and for monitoring recovery. C1 [Kimball, Bruce A.] USDA, APHIS, WS, NWRC,Monell Chem Senses Ctr, 3500 Market St, Philadelphia, PA 19104 USA. [Cohen, Akiva S.; Elkind, Jaclynn] Childrens Hosp Philadelphia, Res Inst, 3615 Civ Ctr Blvd, Philadelphia, PA 19104 USA. [Cohen, Akiva S.] Univ Penn, Perelman Sch Med, Dept Anesthesiol & Crit Care Med, 3615 Civ Ctr Blvd, Philadelphia, PA 19104 USA. [Gordon, Amy R.; Opiekun, Maryanne; Martin, Talia; Lundstroem, Johan N.; Beauchamp, Gary K.] Monell Chem Senses Ctr, 3500 Market St, Philadelphia, PA 19104 USA. [Gordon, Amy R.; Lundstroem, Johan N.] Karolinska Inst, Dept Clin Neurosci, Nobels Vag 9, S-17177 Stockholm, Sweden. RP Kimball, BA (reprint author), USDA, APHIS, WS, NWRC,Monell Chem Senses Ctr, 3500 Market St, Philadelphia, PA 19104 USA. EM bruce.a.kimball@aphis.usda.gov RI Lundstrom, Johan/E-9482-2014 OI Lundstrom, Johan/0000-0002-3529-8981 FU US Army Telemedicine and Advanced Technology Research Center [W81XWH-12-2-0081, EDMS 5584]; NIH [R37 HD59288, RO1 NS069629]; Knut and Alice Wallenberg Foundation [2012.0141] FX This work was supported in part by US Army Telemedicine and Advanced Technology Research Center grant W81XWH-12-2-0081, EDMS 5584, "Odor signals of immune activation and CNS inflammation" (G.K.B., M.O.). Additional support was provided by NIH grants R37 HD59288 and RO1 NS069629 (A.S.C.) and the Knut and Alice Wallenberg Foundation (2012.0141; J.N.L.). NR 64 TC 1 Z9 2 U1 9 U2 14 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0379-864X EI 1464-3553 J9 CHEM SENSES JI Chem. Senses PD JUN PY 2016 VL 41 IS 5 BP 407 EP 414 DI 10.1093/chemse/bjw014 PG 8 WC Behavioral Sciences; Food Science & Technology; Neurosciences; Physiology SC Behavioral Sciences; Food Science & Technology; Neurosciences & Neurology; Physiology GA DR2MU UT WOS:000379739400003 PM 26926034 ER PT J AU Rasooly, R Bruck, HA Balsam, J Prickril, B Ossandon, M Rasooly, A AF Rasooly, Reuven Bruck, Hugh Alan Balsam, Joshua Prickril, Ben Ossandon, Miguel Rasooly, Avraham TI Improving the Sensitivity and Functionality of Mobile Webcam-Based Fluorescence Detectors for Point-of-Care Diagnostics in Global Health SO DIAGNOSTICS LA English DT Review DE webcams; CCD cameras; mobile phones; fluorescence imaging; flow cytometry; rare cells; resource-poor settings; image enhancement; background subtraction; pixel binning; global health ID STAPHYLOCOCCAL-ENTEROTOXIN-B; LINKED IMMUNOSORBENT ASSAYS; IMAGING FLOW CYTOMETER; ARRAY BIOSENSOR; CELL-PHONE; CHEMILUMINESCENCE DETECTION; ISOTHERMAL AMPLIFICATION; COLORIMETRIC DETECTION; MOLECULAR DIAGNOSTICS; MICROFLUIDIC DEVICE AB Resource-poor countries and regions require effective, low-cost diagnostic devices for accurate identification and diagnosis of health conditions. Optical detection technologies used for many types of biological and clinical analysis can play a significant role in addressing this need, but must be sufficiently affordable and portable for use in global health settings. Most current clinical optical imaging technologies are accurate and sensitive, but also expensive and difficult to adapt for use in these settings. These challenges can be mitigated by taking advantage of affordable consumer electronics mobile devices such as webcams, mobile phones, charge-coupled device (CCD) cameras, lasers, and LEDs. Low-cost, portable multi-wavelength fluorescence plate readers have been developed for many applications including detection of microbial toxins such as C. Botulinum A neurotoxin, Shiga toxin, and S. aureus enterotoxin B (SEB), and flow cytometry has been used to detect very low cell concentrations. However, the relatively low sensitivities of these devices limit their clinical utility. We have developed several approaches to improve their sensitivity presented here for webcam based fluorescence detectors, including (1) image stacking to improve signal-to-noise ratios; (2) lasers to enable fluorescence excitation for flow cytometry; and (3) streak imaging to capture the trajectory of a single cell, enabling imaging sensors with high noise levels to detect rare cell events. These approaches can also help to overcome some of the limitations of other low-cost optical detection technologies such as CCD or phone-based detectors (like high noise levels or low sensitivities), and provide for their use in low-cost medical diagnostics in resource-poor settings. C1 [Rasooly, Reuven] USDA, Western Reg Res Ctr, Agr Res Serv, Albany, CA 94706 USA. [Bruck, Hugh Alan] UMCP, Dept Mech Engn, College Pk, MD 20742 USA. [Balsam, Joshua] FDA, Div Chem & Toxicol Devices, Off Vitro Diagnost & Radiol Hlth, Silver Spring, MD 20993 USA. [Prickril, Ben; Ossandon, Miguel; Rasooly, Avraham] NCI, Rockville, MD 20850 USA. RP Rasooly, A (reprint author), NCI, Rockville, MD 20850 USA. EM reuven.rasooly@ars.usda.gov; bruck@umd.edu; joshua.balsam@fda.hhs.gov; ben.prickril@nih.gov; ossandom@mail.nih.gov; rasoolya@mail.nih.gov NR 151 TC 0 Z9 0 U1 11 U2 15 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2075-4418 J9 DIAGNOSTICS JI Diagnostics PD JUN PY 2016 VL 6 IS 2 AR 19 DI 10.3390/diagnostics6020019 PG 21 WC Medicine, General & Internal SC General & Internal Medicine GA DR6VL UT WOS:000380039300006 ER PT J AU Love, BG Cane, JH AF Love, Byron G. Cane, James H. TI Limited direct effects of a massive wildfire on its sagebrush steppe bee community SO ECOLOGICAL ENTOMOLOGY LA English DT Article DE Apoidea; disturbance; Great Basin; insects; sagebrush steppe ID NESTING RESOURCES; FORAGING RANGES; SOLITARY BEES; PAN TRAP; FIRE; HYMENOPTERA; PLANT; RESPONSES; DIVERSITY; APIFORMES AB 1. Fire can affect bees directly through exposure to heat and smoke. Direct effects include mortality, injury, and displacement affecting at most two generations adults and any immature progeny present during the fire. To study the direct effects of fire on bees, two criteria must be met. First, bees must be sampled soon after the fire event, before colonists arrive from outside the burn. Second, sampling locations must be far enough into the burned habitat to ensure that bees observed are survivors, and not foragers nesting outside the burn. 2. Bees were systematically sampled far inside (>7 km) and outside the burn perimeter immediately following a massive wildfire that burned primarily at night in sagebrush steppe habitat. Because adult females sleep in their nests, it was hypothesised that females of species with nests >10 cm underground would be safe from lethal heat. Whereas females with shallow or above-ground nests would be vulnerable. It was also hypothesised that fire would kill proportionately more males, as they typically sleep above ground. 3. Adult bees were present at all burned sample sites 14 and 21 days after the fire started, Many females were observed transporting pollen, indicative of active nest provisioning. Among the guild of bees surveyed at wild sunflowers (the only surviving flowering plant), fewer species were active within the burn. Guild composition was significantly altered, particularly by loss or depletion of several (but not all) sunflower specialists. Sex ratios did not shift, possibly due to surviving males aggregating in remaining patches of sunflowers. C1 [Love, Byron G.; Cane, James H.] ARS, USDA, Pollinating Insect Res Unit, Logan, UT USA. [Love, Byron G.] Utah State Univ, Dept Biol, Logan, UT 84322 USA. RP Love, BG (reprint author), USDA, Pollinating Insect Res Unit, 1410 North 800 East, Logan, UT 84341 USA. EM byron.love@ars.usda.gov NR 70 TC 1 Z9 1 U1 9 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0307-6946 EI 1365-2311 J9 ECOL ENTOMOL JI Ecol. Entomol. PD JUN PY 2016 VL 41 IS 3 BP 317 EP 326 DI 10.1111/een.12304 PG 10 WC Entomology SC Entomology GA DR5KO UT WOS:000379942100010 ER PT J AU Fhaner, M Hwang, HS Winkler-Moser, JK Bakota, EL Liu, SX AF Fhaner, Matthew Hwang, Hong-Sik Winkler-Moser, Jill K. Bakota, Erica L. Liu, Sean X. TI Protection of fish oil from oxidation with sesamol SO EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY LA English DT Article DE Antioxidant; Fish oil; Omega-3 oil; Sesamol ID POLYUNSATURATED FATTY-ACIDS; ANTIOXIDANT ACTIVITY; DOCOSAHEXAENOIC ACID; FRYING TEMPERATURE; VOLATILE COMPOUNDS; LIPID OXIDATION; TUNA OIL; STORAGE; STABILITY; PRODUCTS AB The aim of this study was to determine whether sesamol may provide antioxidant protection for marine omega-3 fatty acids. We tested the effectiveness of sesamol at two temperatures, 30 and 50 degrees C and compared its antioxidant activity with butylated hydroxytoluene (BHT), a commercial rosemary extract, and gamma-oryzanol. Each antioxidant system was tested at two concentrations, 0.84 and 8.4 mM except for BHT of which the concentration was fixed at 0.84 mM BHT (200 ppm, the regulatory limit allowed in oils). 0.84 mM phenolic diterpenes in the rosemary extract protected fish oil very well. 0.84 mM sesamol was not as good as the rosemary extract as indicated by peroxide values, conjugated diene values, eicosapentaenoic acid(EPA) and docosahexaenoic acid(DHA) levels, and volatile compounds formation. However, 8.4 mM sesamol performed better than the rosemary extract containing 8.4 mM phenolic diterpenes in peroxide values and headspace volatiles compounds during storage of the stripped fish oil for 14 days at 30 and 50 degrees C, while it showed similar results as the rosemary extract in conjugated diene values and EPA and DHA levels. Gamma-oryzanol was also evaluated for protection of fish oil. However, it was found that gamma-oryzanol was less effective than the other antioxidants tested in this study. Practical applications: At 8.4 mM (0.13 wt%), sesamol, a natural antioxidant, showed better than or comparable antioxidant activity to a commercial rosemary extract toward fish oil during storage at 30 and 50 degrees C. Considering problems of flavor, odor, and color associated with a rosemary extract and that a rosemary extract product is typically sold as a solution containing only a few percent of active ingredients for oil applications, such as omega-3 oil supplements and frying oil due to the low solubility of active ingredients in oil, sesamol offers advantages over the rosemary extract as it can be used as a pure form without mixing with other ingredients. C1 [Fhaner, Matthew] Univ Michigan, Dept Chem & Biochem, Flint, MI 48503 USA. [Hwang, Hong-Sik; Winkler-Moser, Jill K.; Bakota, Erica L.; Liu, Sean X.] ARS, USDA, Natl Ctr Agr Utilizat Res, Funct Foods Res, Peoria, IL USA. RP Hwang, HS (reprint author), ARS, Natl Ctr Agr Utilizat Res, Funct Foods Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Hongsik.hwang@ars.usda.gov NR 44 TC 0 Z9 0 U1 13 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1438-7697 EI 1438-9312 J9 EUR J LIPID SCI TECH JI Eur. J. Lipid Sci. Technol. PD JUN PY 2016 VL 118 IS 6 BP 885 EP 897 DI 10.1002/ejlt.201500185 PG 13 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA DR6PS UT WOS:000380024400005 ER PT J AU Coble, AA Marcarelli, AM Kane, ES Toczydlowski, D Stottlemyer, R AF Coble, Ashley A. Marcarelli, Amy M. Kane, Evan S. Toczydlowski, David Stottlemyer, Robert TI Temporal patterns of dissolved organic matter biodegradability are similar across three rivers of varying size SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article DE dissolved organic carbon loads; dissolved organic matter composition; temporal variability; temperate tributaries ID SNOWPACK ION ACCUMULATION; FRESH-WATER ECOSYSTEMS; LAKE-SUPERIOR; CHEMICAL-COMPOSITION; AQUATIC ECOSYSTEMS; HEADWATER STREAMS; NORTHERN MICHIGAN; RESIDENCE TIME; BOREAL LAKES; LAND-COVER AB Dissolved organic matter (DOM) composition may be an important determinant of its fate in freshwaters, but little is known about temporal variability in DOM composition and the biodegradability of DOM in northern temperate watersheds. We measured biodegradable dissolved organic carbon (BDOC) via incubation assays and DOM composition using optical indices on 11 dates in three Lake Superior tributaries. Percent BDOC (%BDOC) and BDOC concentrations were seasonally synchronous across these watersheds, despite that they vary in size by orders of magnitude (1.7 to 3400km(2)). Relative to %BDOC, BDOC concentrations were more tightly constrained among sites on any given date. BDOC also varied within seasons; for example, %BDOC on two different dates in winter were among the highest (29% and 54%) and lowest (0%) values observed for each site (overall %BDOC range: 0 to 72%). DOM composition varied the most among tributaries during a summer storm event when BDOC (both as percent and concentration) was elevated but was remarkably similar among tributaries during fall, spring, and winter. Multivariate models identified humic-like and tryptophan-like fluorophores as predictors of %BDOC, but DOM composition only described 21% of the overall variation in %BDOC. Collectively, these three rivers exported similar to 18GgCyr(-1) as DOC and similar to 2GgCyr(-1) as BDOC, which corresponded to 9 to 17% of annual DOC exported in biodegradable form. Our results suggest much of the C exported from these northern temperate watersheds may be biodegradable within 28days and that large pulses of labile DOM can be exported during storm events and spring snowmelt. C1 [Coble, Ashley A.; Marcarelli, Amy M.; Toczydlowski, David; Stottlemyer, Robert] Michigan Technol Univ, Dept Biol Sci, Houghton, MI 49931 USA. [Coble, Ashley A.] Univ New Hampshire, Dept Nat Resources & Environm, Durham, NH 03824 USA. [Kane, Evan S.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. [Kane, Evan S.] US Forest Serv, Northern Res Stn, Houghton, MI USA. RP Coble, AA (reprint author), Michigan Technol Univ, Dept Biol Sci, Houghton, MI 49931 USA.; Coble, AA (reprint author), Univ New Hampshire, Dept Nat Resources & Environm, Durham, NH 03824 USA. EM Ashley.Coble@unh.edu OI Marcarelli, Amy/0000-0002-4175-9211 FU NASA Michigan Space Grant Consortium; U.S.D.A. McIntire-Stennis; Michigan Technological University's Research Excellence Fund; Huron Mountain Wildlife Foundation; Michigan Tech Ecosystem Science Center; National Science Foundation's GK-12 Global Watershed program [DGE-0841073] FX Funding for this research was provided by NASA Michigan Space Grant Consortium, U.S.D.A. McIntire-Stennis funding to Michigan Technological University, a Research Seed Grant from Michigan Technological University's Research Excellence Fund, the Huron Mountain Wildlife Foundation, and the Michigan Tech Ecosystem Science Center. A.A.C. was also supported with a fellowship from the National Science Foundation's GK-12 Global Watershed program (award DGE-0841073). We thank E. Collins, B. Danhoff, K. Heiden, and T. Matthys for assistance in the field and lab and T. Veverica and K. Meingast for assistance with analysis of fluorescence characteristics. We also thank I. Buffam and four anonymous reviewers for helpful comments which improved the quality of this manuscript. Additional data supporting our findings are provided in the supporting information. This is contribution 37 of the Great Lakes Research Center at Michigan Tech. NR 80 TC 1 Z9 1 U1 13 U2 19 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD JUN PY 2016 VL 121 IS 6 BP 1617 EP 1631 DI 10.1002/2015JG003218 PG 15 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA DR5TJ UT WOS:000379965000015 ER PT J AU Tang, CR Yang, M Fang, YJ Luo, YF Gao, SH Xiao, XH An, ZW Zhou, BH Zhang, B Tan, XY Yeang, HY Qin, YX Yang, JH Lin, Q Mei, HL Montoro, P Long, XY Qi, JY Hua, YW He, ZL Sun, M Li, WJ Zeng, X Cheng, H Liu, Y Yang, J Tian, WM Zhuang, NS Zeng, RZ Li, DJ He, P Li, Z Zou, Z Li, SL Li, CJ Wang, JX Wei, D Lai, CQ Luo, W Yu, J Hu, SN Huang, HS AF Tang, Chaorong Yang, Meng Fang, Yongjun Luo, Yingfeng Gao, Shenghan Xiao, Xiaohu An, Zewei Zhou, Binhui Zhang, Bing Tan, Xinyu Yeang, Hoong-Yeet Qin, Yunxia Yang, Jianghua Lin, Qiang Mei, Hailiang Montoro, Pascal Long, Xiangyu Qi, Jiyan Hua, Yuwei He, Zilong Sun, Min Li, Wenjie Zeng, Xia Cheng, Han Liu, Ying Yang, Jin Tian, Weimin Zhuang, Nansheng Zeng, Rizhong Li, Dejun He, Peng Li, Zhe Zou, Zhi Li, Shuangli Li, Chenji Wang, Jixiang Wei, Dong Lai, Chao-Qiang Luo, Wei Yu, Jun Hu, Songnian Huang, Huasun TI The rubber tree genome reveals new insights into rubber production and species adaptation SO NATURE PLANTS LA English DT Article ID HEVEA-BRASILIENSIS MUELL.; NATURAL-RUBBER; ETHYLENE STIMULATION; ELONGATION-FACTOR; LATEX CELLS; TRANSCRIPTOME ANALYSIS; PATHWAY GENES; BIOSYNTHESIS; PLANT; EXPRESSION AB The Para rubber tree (Hevea brasiliensis) is an economically important tropical tree species that produces natural rubber, an essential industrial raw material. Here we present a high-quality genome assembly of this species (1.37 Gb, scaffold N50 = 1.28 Mb) that covers 93.8% of the genome (1.47 Gb) and harbours 43,792 predicted protein-coding genes. A striking expansion of the REF/SRPP (rubber elongation factor/small rubber particle protein) gene family and its divergence into several laticifer-specific isoforms seem crucial for rubber biosynthesis. The REF/SRPP family has isoforms with sizes similar to or larger than SRPP1 (204 amino acids) in 17 other plants examined, but no isoforms with similar sizes to REF1 (138 amino acids), the predominant molecular variant. A pivotal point in Hevea evolution was the emergence of REF1, which is located on the surface of large rubber particles that account for 93% of rubber in the latex (despite constituting only 6% of total rubber particles, large and small). The stringent control of ethylene synthesis under active ethylene signalling and response in laticifers resolves a longstanding mystery of ethylene stimulation in rubber production. Our study, which includes the re-sequencing of five other Hevea cultivars and extensive RNA-seq data, provides a valuable resource for functional genomics and tools for breeding elite Hevea cultivars. C1 [Tang, Chaorong; Fang, Yongjun; Xiao, Xiaohu; An, Zewei; Zhou, Binhui; Qin, Yunxia; Yang, Jianghua; Long, Xiangyu; Qi, Jiyan; Hua, Yuwei; Zeng, Xia; Cheng, Han; Tian, Weimin; Zeng, Rizhong; Li, Dejun; He, Peng; Li, Zhe; Zou, Zhi; Luo, Wei; Huang, Huasun] CATAS, Rubber Res Inst, Danzhou 571737, Peoples R China. [Yang, Meng; Luo, Yingfeng; Tan, Xinyu; Lin, Qiang; He, Zilong; Li, Chenji; Wang, Jixiang; Wei, Dong; Yu, Jun; Hu, Songnian] Chinese Acad Sci, Beijing Inst Genom, CAS Key Lab Genome Sci & Informat, Beijing 100101, Peoples R China. [Yang, Meng; Mei, Hailiang; He, Zilong; Zhuang, Nansheng; Hu, Songnian] Univ Chinese Acad Sci, Beijing, Peoples R China. [Zhou, Binhui] Hainan Univ, Coll Agron, Haikou 570228, Peoples R China. [Sun, Min; Li, Wenjie; Liu, Ying; Yang, Jin; Li, Shuangli] Chinese Acad Sci, Beijing Inst Genom, Core Genom Facil, Beijing 100101, Peoples R China. [Yeang, Hoong-Yeet] Bukit Bandar Raya, Kuala Lumpur 59100, Malaysia. [Montoro, Pascal] UMR AGAP, CIRAD, F-34398 Montpellier, France. [Lai, Chao-Qiang] Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA. RP Tang, CR; Huang, HS (reprint author), CATAS, Rubber Res Inst, Danzhou 571737, Peoples R China.; Yu, J; Hu, SN (reprint author), Chinese Acad Sci, Beijing Inst Genom, CAS Key Lab Genome Sci & Informat, Beijing 100101, Peoples R China.; Hu, SN (reprint author), Univ Chinese Acad Sci, Beijing, Peoples R China. EM chaorongtang@126.com; yujun@big.ac.cn; husn@big.ac.cn; xjshhs@163.com FU Fundamental Research Funds for Rubber Research Institute, CATAS; 863 program [2013AA102605] FX We thank E. (Liansheng) Zheng for critically reading the manuscript. This research was financially supported by grants from the Fundamental Research Funds for Rubber Research Institute, CATAS, and the 863 program (2013AA102605). NR 65 TC 14 Z9 15 U1 11 U2 16 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2055-026X EI 2055-0278 J9 NAT PLANTS JI Nat. Plants PD JUN PY 2016 VL 2 IS 6 AR 16073 DI 10.1038/NPLANTS.2016.73 PG 10 WC Plant Sciences SC Plant Sciences GA DS1HH UT WOS:000380346000010 PM 27255837 ER PT J AU He, F Yoo, S Wang, DF Kumari, S Gerstein, M Ware, D Maslov, S AF He, Fei Yoo, Shinjae Wang, Daifeng Kumari, Sunita Gerstein, Mark Ware, Doreen Maslov, Sergei TI Large-scale atlas of microarray data reveals the distinct expression landscape of different tissues in Arabidopsis SO PLANT JOURNAL LA English DT Article DE Arabidopsis; expression data integration; metadata annotation; global transcriptional landscape; automatic reconstruction of missing metadata; re-use of public expression data ID GENE-EXPRESSION; PROFILING REVEALS; MAP; GENOME; PLANTS; TRANSCRIPTOME; NORMALIZATION; BIOSYNTHESIS; REGENERATION; COEXPRESSION AB Transcriptome data sets from thousands of samples of the model plant Arabidopsis thaliana have been collectively generated by multiple individual labs. Although integration and meta-analysis of these samples has become routine in the plant research community, it is often hampered by a lack of metadata or differences in annotation styles of different labs. In this study, we carefully selected and integrated 6057 Arabidopsis microarray expression samples from 304 experiments deposited to the Gene Expression Omnibus (GEO) at the National Center for Biotechnology Information (NCBI). Metadata such as tissue type, growth conditions and developmental stage were manually curated for each sample. We then studied the global expression landscape of the integrated data set and found that samples of the same tissue tend to be more similar to each other than to samples of other tissues, even in different growth conditions or developmental stages. Root has the most distinct transcriptome, compared with aerial tissues, but the transcriptome of cultured root is more similar to the transcriptome of aerial tissues, as the cultured root samples lost their cellular identity. Using a simple computational classification method, we showed that the tissue type of a sample can be successfully predicted based on its expression profile, opening the door for automatic metadata extraction and facilitating the re-use of plant transcriptome data. As a proof of principle, we applied our automated annotation pipeline to 708 RNA-seq samples from public repositories and verified the accuracy of our predictions with sample metadata provided by the authors. C1 [He, Fei; Maslov, Sergei] Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA. [Yoo, Shinjae] Brookhaven Natl Lab, Computat Sci Ctr, Upton, NY 11973 USA. [Yoo, Shinjae] SUNY Stony Brook, Inst Adv Computat Sci, Stony Brook, NY 11794 USA. [Wang, Daifeng; Gerstein, Mark] Yale Univ, Program Computat Biol & Bioinformat, New Haven, CT 06520 USA. [Kumari, Sunita; Ware, Doreen] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA. [Ware, Doreen] USDA ARS, USDA ARS NEA Plant Soil & Nutr Lab Res Unit, Ithaca, NY 14853 USA. [Maslov, Sergei] Carl R Woese Inst Genom Biol, Dept Bioengn, Urbana, IL 61801 USA. [Maslov, Sergei] Univ Illinois, Natl Ctr Supercomp Applicat, Urbana, IL 61801 USA. RP Maslov, S (reprint author), Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA.; Maslov, S (reprint author), Carl R Woese Inst Genom Biol, Dept Bioengn, Urbana, IL 61801 USA.; Maslov, S (reprint author), Univ Illinois, Natl Ctr Supercomp Applicat, Urbana, IL 61801 USA. EM maslov@illinois.edu RI He, Fei/C-5912-2015; OI He, Fei/0000-0002-1165-3248; Maslov, Sergei/0000-0002-3701-492X FU Office of Biological and Environmental Research of the US Department of Energy [ELS165] FX This work was supported by Grant ELS165 from the Office of Biological and Environmental Research of the US Department of Energy. Jason Baumohl from Lawrence Berkeley National Laboratory provided helpful suggestions for this study. We would like to thank both reviewers for their insightful comments on the article. The authors declare that there are no conflicts of interest. NR 56 TC 3 Z9 3 U1 10 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0960-7412 EI 1365-313X J9 PLANT J JI Plant J. PD JUN PY 2016 VL 86 IS 6 BP 472 EP 480 DI 10.1111/tpj.13175 PG 9 WC Plant Sciences SC Plant Sciences GA DR9KB UT WOS:000380214500003 PM 27015116 ER PT J AU Knight-Jones, TJD Robinson, L Charleston, B Rodriguez, LL Gay, CG Sumption, KJ Vosloo, W AF Knight-Jones, T. J. D. Robinson, L. Charleston, B. Rodriguez, L. L. Gay, C. G. Sumption, K. J. Vosloo, W. TI Global Foot-and-Mouth Disease Research Update and Gap Analysis: 1-Overview of Global Status and Research Needs SO TRANSBOUNDARY AND EMERGING DISEASES LA English DT Article DE Foot-and-mouth disease; FMD; gap analysis; global; research ID IMPACTS; FMD; SURVEILLANCE; VIRUS AB The Global Foot-and-mouth disease (FMD) Research Alliance periodically reviews the state of FMD research to assess progress and to identify new priorities. In this supplement we provide an update of global FMD research, comprising (i) this overview paper, which includes background information with key findings, and papers covering (ii) epidemiology, wildlife and economics, (iii) vaccines, (iv) diagnostics, (v) biotherapeutics and disinfectants, (vi) immunology and (vii) pathogenesis and molecular biology. FMD research publications were reviewed (2011-2015) and activity updates were obtained from 33 FMD research institutes from around the world. Although a continual threat, FMD has been effectively controlled in much of the world using existing tools. However, control remains a challenge in most developing countries, where little has been done to understand the ongoing burden of FMD. More research is needed to support control in endemically infected countries, particularly robust field studies. Traditional FMD vaccines have several limitations including short duration and spectrum of protection, cold chain requirements, and the costs and biosecurity risks associated with vaccine production. Significant progress has been made in the development of novel vaccine candidates, particularly in the use of recombinant vaccines and virus-like particles as an alternative to traditional inactivated whole virus vaccines. Continued investment is needed to turn these developments into improved vaccines produced at scale. Increased knowledge of cellular and mucosal immunity would benefit vaccine development, as would further advances in our ability to enhance vaccine capsid stability. Developments in molecular biology and phylogenetics underlie many of the recent advances in FMD research, including improved vaccines and diagnostics, and improved understanding of FMD epidemiology. Tools for genetic analyses continue to become both more powerful and more affordable enabling them to be used to address an ever-expanding range of questions. This rapidly advancing field potentiates many areas of FMD research and should be prioritized. C1 [Knight-Jones, T. J. D.] Int Livestock Res Inst, Katima Mulilo Rd, Lusaka, Zambia. [Robinson, L.] Insight Editing London, London, England. [Charleston, B.] Pirbright Inst, Pirbright, England. [Rodriguez, L. L.] ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY USA. [Gay, C. G.] ARS, USDA, Natl Program Anim Hlth 103, Beltsville, MD USA. [Sumption, K. J.] European Commiss Control FMD EuFMD, FAO, Rome, Italy. [Vosloo, W.] CSIRO Biosecur Flagship, Australian Anim Hlth Lab, Geelong, Vic, Australia. RP Knight-Jones, TJD (reprint author), Int Livestock Res Inst, Katima Mulilo Rd, Lusaka, Zambia. EM t.knight-jones@cgiar.org RI Institute, Pirbright/K-4476-2014 FU EuFMD FX The authors gratefully acknowledge the input of all scientists and institutes that responded to the requests for research activity updates. The authors are also indebted to the considerable efforts of various members of the GFRA committee. Thanks also go to members of the European commission for the control of FMD (EuFMD) Special Committee for Research and Programme Development (SCRPD), and Jacquelyn Horsington and Nagendra Singanallur from CSIRO. This work was funded by EuFMD. NR 24 TC 0 Z9 0 U1 10 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1865-1674 EI 1865-1682 J9 TRANSBOUND EMERG DIS JI Transbound. Emerg. Dis. PD JUN PY 2016 VL 63 SU 1 SI SI BP 3 EP 13 DI 10.1111/tbed.12528 PG 11 WC Infectious Diseases; Veterinary Sciences SC Infectious Diseases; Veterinary Sciences GA DR5KY UT WOS:000379943100001 PM 27320162 ER PT J AU Knight-Jones, TJD Robinson, L Charleston, B Rodriguez, LL Gay, CG Sumption, KJ Vosloo, W AF Knight-Jones, T. J. D. Robinson, L. Charleston, B. Rodriguez, L. L. Gay, C. G. Sumption, K. J. Vosloo, W. TI Global Foot-and-Mouth Disease Research Update and Gap Analysis: 2-Epidemiology, Wildlife and Economics SO TRANSBOUNDARY AND EMERGING DISEASES LA English DT Article DE FMD; foot-and-mouth disease; epidemiology; wildlife; economics; gap analysis ID CALF PRODUCERS BELIEFS; VACCINATE-TO-LIVE; EMERGENCY VACCINATION; ANIMAL-DISEASE; TRANSMISSION DYNAMICS; MOVEMENT RESTRICTIONS; PASSIVE SURVEILLANCE; SMALLHOLDER FARMERS; LIVESTOCK DISEASES; SOUTHERN CAMBODIA AB We assessed knowledge gaps in foot-and-mouth disease (FMD) research, and in this study, we consider (i) epidemiology, (ii) wildlife and (iii) economics. The study took the form of a literature review (2011-2015) combined with research updates collected in 2014 from 33 institutes from across the world. Findings were used to identify priority areas for future FMD research. During 2011-2015, modelling studies were dominant in the broad field of epidemiology; however, continued efforts are required to develop robust models for use during outbreaks in FMD-free countries, linking epidemiologic and economics models. More guidance is needed for both the evaluation and the setting of targets for vaccine coverage, population immunity and vaccine field efficacy. Similarly, methods for seroprevalence studies need to be improved to obtain more meaningful outputs that allow comparison across studies. To inform control programmes in endemic countries, field trials assessing the effectiveness of vaccination in extensive smallholder systems should be performed to determine whether FMD can be controlled with quality vaccines in settings where implementing effective biosecurity is challenging. Studies need to go beyond measuring only vaccine effects and should extend our knowledge of the impact of FMD and increase our understanding of how to maximize farmer participation in disease control. Where wildlife reservoirs of virus exist, particularly African Buffalo, we need to better understand when and under what circumstances transmission to domestic animals occurs in order to manage this risk appropriately, considering the impact of control measures on livelihoods and wildlife. For settings where FMD eradication is unfeasible, further ground testing of commodity-based trade is recommended. A thorough review of global FMD control programmes, covering successes and failures, would be extremely valuable and could be used to guide other control programmes. C1 [Knight-Jones, T. J. D.] Int Livestock Res Inst, Lusaka, Zambia. [Robinson, L.] Insight Editing London, London, England. [Charleston, B.] Pirbright Inst, Pirbright, England. [Rodriguez, L. L.] ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY USA. [Gay, C. G.] ARS, USDA, Natl Program Anim Hlth 103, Beltsville, MD USA. [Sumption, K. J.] European Commiss Control FMD EuFMD, FAO, Rome, Italy. [Vosloo, W.] CSIRO Biosecur Flagship, Australian Anim Hlth Lab, Geelong, Vic, Australia. RP Knight-Jones, TJD (reprint author), WorldFish, Int Livestock Res Inst, Lusaka, Zambia. EM t.knight-jones@cgiar.org RI Institute, Pirbright/K-4476-2014 FU EuFMD FX The authors gratefully acknowledge the input of all scientists and institutes that responded to the requests for research activity updates. The authors are also indebted to the considerable efforts of various members of the GFRA committee. Thanks also go to Keith Sumption and members of the European Commission for the Control of FMD (EuFMD) Special Committee for Research and Programme Development (SCRPD), and Jacquelyn Horsington and Nagendra Singanallur from CSIRO. This work was funded by EuFMD. NR 150 TC 0 Z9 0 U1 13 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1865-1674 EI 1865-1682 J9 TRANSBOUND EMERG DIS JI Transbound. Emerg. Dis. PD JUN PY 2016 VL 63 SU 1 SI SI BP 14 EP 29 DI 10.1111/tbed.12522 PG 16 WC Infectious Diseases; Veterinary Sciences SC Infectious Diseases; Veterinary Sciences GA DR5KY UT WOS:000379943100002 PM 27320163 ER PT J AU Robinson, L Knight-Jones, TJD Charleston, B Rodriguez, LL Gay, CG Sumption, KJ Vosloo, W AF Robinson, L. Knight-Jones, T. J. D. Charleston, B. Rodriguez, L. L. Gay, C. G. Sumption, K. J. Vosloo, W. TI Global Foot-and-Mouth Disease Research Update and Gap Analysis: 3-Vaccines SO TRANSBOUNDARY AND EMERGING DISEASES LA English DT Article DE FMD; foot-and-mouth disease; vaccines; vaccinology; gap analysis ID CYATHULAE-OFFICINALIS-KUAN; NONSTRUCTURAL PROTEIN 2B; IMMUNE-RESPONSES; CROSS-PROTECTION; QUALITY-CONTROL; CHITOSAN NANOPARTICLES; EMERGENCY VACCINATION; MAMMALIAN-CELLS; VIRUS SEROTYPE; EAST-AFRICA AB This study assessed research knowledge gaps in the field of FMDV (foot-and-mouth disease virus) vaccines. The study took the form of a literature review (2011-15) combined with research updates collected in 2014 from 33 institutes from across the world. Findings were used to identify priority areas for future FMD vaccine research. Vaccines play a vital role in FMD control, used both to limit the spread of the virus during epidemics in FMD-free countries and as the mainstay of disease management in endemic regions, particularly where sanitary controls are difficult to apply. Improvements in the performance or cost-effectiveness of FMD vaccines will allow more widespread and efficient disease control. FMD vaccines have changed little in recent decades, typically produced by inactivation of whole virus, the quantity and stability of the intact viral capsids in the final preparation being key for immunogenicity. However, these are exciting times and several promising novel FMD vaccine candidates have recently been developed. This includes the first FMD vaccine licensed for manufacture and use in the USA; this adenovirus-vectored FMD vaccine causes invivo expression of viral capsids in vaccinated animals. Another promising vaccine candidate comprises stabilized empty FMDV capsids produced invitro in a baculovirus expression system. Recombinant technologies are also being developed to improve otherwise conventionally produced inactivated vaccines, for example, by creating a chimeric vaccine virus to increase capsid stability and by inserting sequences into the vaccine virus for desired antigen expression. Other important areas of ongoing research include enhanced adjuvants, vaccine quality control procedures and predicting vaccine protection from immune correlates, thus reducing dependency on animal challenge studies. Globally, the degree of independent vaccine evaluation is highly variable, and this is essential for vaccine quality. Previously neglected, the importance of evaluating vaccination programme effectiveness and impact is increasingly being recognized. C1 [Robinson, L.] Insight Editing London, London, England. [Knight-Jones, T. J. D.] Int Livestock Res Inst, Lusaka, Zambia. [Charleston, B.] Pirbright Inst, Pirbright, England. [Rodriguez, L. L.] ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY USA. [Gay, C. G.] ARS, Natl Program Anim Hlth 103, USDA, Beltsville, MD USA. [Sumption, K. J.] European Commiss Control FMD EuFMD, FAO, Rome, Italy. [Vosloo, W.] CSIRO Biosecur Flagship, Australian Anim Hlth Lab, Geelong, Vic, Australia. RP Knight-Jones, TJD (reprint author), WorldFish, Int Livestock Res Inst, Lusaka, Zambia. EM t.knight-jones@cgiar.org RI Institute, Pirbright/K-4476-2014 FU EuFMD FX The authors gratefully acknowledge the input of all scientists and institutes that responded to the requests for research activity updates. The authors are also indebted to the considerable efforts of various members of the GFRA committee. Thanks also go to Keith Sumption and members of the European Commission for the Control of FMD (EuFMD) Special Committee for Research and Programme Development (SCRPD), and Jacquelyn Horsington and Nagendra Singanallur from CSIRO. This work was funded by EuFMD. NR 85 TC 0 Z9 0 U1 10 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1865-1674 EI 1865-1682 J9 TRANSBOUND EMERG DIS JI Transbound. Emerg. Dis. PD JUN PY 2016 VL 63 SU 1 SI SI BP 30 EP 41 DI 10.1111/tbed.12521 PG 12 WC Infectious Diseases; Veterinary Sciences SC Infectious Diseases; Veterinary Sciences GA DR5KY UT WOS:000379943100003 PM 27320164 ER PT J AU Knight-Jones, TJD Robinson, L Charleston, B Rodriguez, LL Gay, CG Sumption, KJ Vosloo, W AF Knight-Jones, T. J. D. Robinson, L. Charleston, B. Rodriguez, L. L. Gay, C. G. Sumption, K. J. Vosloo, W. TI Global Foot-and-Mouth Disease Research Update and Gap Analysis: 4-Diagnostics SO TRANSBOUNDARY AND EMERGING DISEASES LA English DT Article DE foot-and-mouth disease; fmd; diagnostics; review ID MEDIATED ISOTHERMAL AMPLIFICATION; TIME RT-PCR; VIRUS SEROTYPES O; MONOCLONAL-ANTIBODY; RAPID DETECTION; NONSTRUCTURAL PROTEINS; VACCINATED CATTLE; ASIA 1; ELISA; INFECTION AB This study assessed knowledge gaps in foot-and-mouth disease (FMD) research in the field of diagnostics. The study took the form of a literature review (2011-15) combined with research updates collected in 2014 from 33 institutes from around the world. Findings were used to identify priority areas for future FMD research. Molecular and genetic technologies, including sequencing, are developing at an increasing rate both in terms of capability and affordability. These advances potentiate progress in many other fields of research, from vaccine development to epidemiology. The development of RT-LAMP represents an important breakthrough allowing greater use and access to molecular diagnostics. It is now possible to determine virus serotype using PCR, although only for certain virus pools, continued progress is needed to cover the global spectrum of FMD viruses. Progress has also been made in the development of pen-side rapid diagnostics, some with the ability to determine serotype. However, further advances in pen-side serotype or strain determination would benefit both FMD-free countries and endemic countries with limited access to well-resourced laboratories. Novel sampling methods that show promise include air sampling and baited ropes, the latter may aid sampling in wildlife and swine. Studies of infrared thermography for the early detection of FMD have not been encouraging, although investigations are ongoing. Multiplex tests have been developed that are able to simultaneously screen for multiple pathogens with similar clinical signs. Crucial for assessing FMDV freedom, tests exist to detect animals that have been infected with FMDV regardless of vaccination status; however, limitations exist, particularly when testing previously vaccinated animals. Novel vaccines are being developed with complementary DIVA tests for this purpose. Research is also needed to improve the current imprecise approaches to FMD vaccine matching. The development of simple, affordable tests increases access to FMD diagnostics, greatly benefiting regions with limited laboratory capacity. C1 [Knight-Jones, T. J. D.] Int Livestock Res Inst, Lusaka, Zambia. [Robinson, L.] Insight Editing London, London, England. [Charleston, B.] Pirbright Inst, Pirbright, England. [Rodriguez, L. L.] ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY USA. [Gay, C. G.] ARS, USDA, Natl Program Anim Hlth 103, Beltsville, MD USA. [Sumption, K. J.] European Commiss Control FMD EuFMD, FAO, Rome, Italy. [Vosloo, W.] CSIRO Biosecur Flagship, Australian Anim Hlth Lab, Geelong, Vic, Australia. RP Knight-Jones, TJD (reprint author), WorldFish, Int Livestock Res Inst, Lusaka, Zambia. EM t.knight-jones@cgiar.org RI Institute, Pirbright/K-4476-2014 FU EuFMD FX The authors gratefully acknowledge the input of all scientists and institutes that responded to the requests for research activity updates. The authors are also indebted to the considerable efforts of various members of the GFRA committee. Thanks also go to Keith Sumption and members of the European Commission for the Control of FMD (EuFMD) Special Committee for Research and Programme Development (SCRPD), and Jacquelyn Horsington and Nagendra Singanallur from CSIRO. This study was funded by EuFMD. NR 54 TC 0 Z9 0 U1 8 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1865-1674 EI 1865-1682 J9 TRANSBOUND EMERG DIS JI Transbound. Emerg. Dis. PD JUN PY 2016 VL 63 SU 1 SI SI BP 42 EP 48 DI 10.1111/tbed.12523 PG 7 WC Infectious Diseases; Veterinary Sciences SC Infectious Diseases; Veterinary Sciences GA DR5KY UT WOS:000379943100004 PM 27320165 ER PT J AU Robinson, L Knight-Jones, TJD Charleston, B Rodriguez, LL Gay, CG Sumption, KJ Vosloo, W AF Robinson, L. Knight-Jones, T. J. D. Charleston, B. Rodriguez, L. L. Gay, C. G. Sumption, K. J. Vosloo, W. TI Global Foot-and-Mouth Disease Research Update and Gap Analysis: 5-Biotherapeutics and Disinfectants SO TRANSBOUNDARY AND EMERGING DISEASES LA English DT Article DE Foot-and-mouth disease; FMD; biotherapeutics; disinfectants; gap analysis ID CLASSICAL SWINE-FEVER; INFECTION IN-VITRO; VIRUS-INFECTION; PROTECTS SWINE; IFN-ALPHA; REPLICATION; CATTLE; VIVO; INACTIVATION; EXPRESSION AB We assessed knowledge gaps in foot-and-mouth disease (FMD) research. Findings are reported in a series of papers, and in this article, we consider biotherapeutics and disinfectants. The study took the form of a literature review (2011-2015) combined with research updates collected in 2014 from 33 institutes from across the world. Findings were used to identify priority areas for future FMD research. While vaccines will remain the key immunological intervention used against FMD virus (FMDV) for the foreseeable future, it takes a few days for the immune system to respond to vaccination. In an outbreak situation, protection could potentially be provided during this period by the application of rapid, short-acting biotherapeutics, aiming either to stimulate a non-specific antiviral state in the animal or to specifically inhibit a part of the viral life cycle. Certain antiviral cytokines have been shown to promote rapid protection against FMD; however, the effects of different immune-modulators appear to vary across species in ways and for reasons that are not yet understood. Major barriers to the effective incorporation of biotherapeutics into control strategies are cost, limited understanding of their effect on subsequent immune responses to vaccines and uncertainty about their potential impact if used for disease containment. Recent research has highlighted the importance of environmental contamination in FMDV transmission. Effective disinfectants for FMDV have long been available, but research is being conducted to further develop methods for quantitatively evaluating their performance under field, or near-field, conditions. During outbreaks in South Korea in 2010 there was public concern about potential environmental contamination after the mass use of disinfectant and mass burial of culled stock; this should be considered during outbreak contingency planning. C1 [Robinson, L.] Insight Editing London, London, England. [Knight-Jones, T. J. D.] Int Livestock Res Inst, Lusaka, Zambia. [Charleston, B.] Pirbright Inst, Pirbright, England. [Rodriguez, L. L.] ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY USA. [Gay, C. G.] ARS, Natl Program Anim Hlth 103, USDA, Beltsville, MD USA. [Sumption, K. J.] European Commiss Control FMD EuFMD, FAO, Rome, Italy. [Vosloo, W.] CSIRO Biosecur Flagship, Australian Anim Hlth Lab, Geelong, Vic, Australia. RP Knight-Jones, TJD (reprint author), WorldFish, Int Livestock Res Inst, Lusaka, Zambia. EM t.knight-jones@cgiar.org RI Institute, Pirbright/K-4476-2014 FU EuFMD FX The authors gratefully acknowledge the input of all scientists and institutes that responded to the requests for research activity updates. The authors are also indebted to the considerable efforts of various members of the GFRA committee. Thanks also go to members of the European Commission for the Control of FMD (EuFMD) Special Committee for Research and Programme Development (SCRPD), and Jacquelyn Horsington and Nagendra Singanallur from CSIRO. This study was funded by EuFMD. NR 36 TC 0 Z9 0 U1 3 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1865-1674 EI 1865-1682 J9 TRANSBOUND EMERG DIS JI Transbound. Emerg. Dis. PD JUN PY 2016 VL 63 SU 1 SI SI BP 49 EP 55 DI 10.1111/tbed.12519 PG 7 WC Infectious Diseases; Veterinary Sciences SC Infectious Diseases; Veterinary Sciences GA DR5KY UT WOS:000379943100005 PM 27320166 ER PT J AU Robinson, L Knight-Jones, TJD Charleston, B Rodriguez, LL Gay, CG Sumption, KJ Vosloo, W AF Robinson, L. Knight-Jones, T. J. D. Charleston, B. Rodriguez, L. L. Gay, C. G. Sumption, K. J. Vosloo, W. TI Global Foot-and-Mouth Disease Research Update and Gap Analysis: 6-Immunology SO TRANSBOUNDARY AND EMERGING DISEASES LA English DT Article DE foot-and-mouth disease; immunology; immunity ID DENDRITIC CELLS; VIRUS-INFECTION; MATERNAL ANTIBODIES; RESPIRATORY-TRACT; ALPHA RESPONSES; IFN-ALPHA; T-CELLS; CATTLE; VACCINATION; VACCINES AB This study assessed gaps and priorities for FMDV (foot-and-mouth disease virus) research in the field of immunology. The study took the form of a literature review (2011-15) combined with research updates collected in 2014 from 33 institutes from across the world. Findings were used to identify priority areas for future FMD research. Improved understanding of FMDV immunology facilitates the development of vaccines, adjuvants and diagnostic tests, and will allow better assessment and prediction of vaccine potency and match, with reduced use of animals, particularly large animals, in experimental studies. Continued characterization of the immune systems of several FMD host species has underpinned substantial advances in knowledge of their interaction with FMDV. Recent studies have shed light on the mechanisms underlying formation of the bovine B- and T-cell response; there is also a greater understanding of the significance of non-neutralizing antibodies during FMDV infection and the interactions of antibody-bound virus with immune cells. This knowledge is directly relevant to vaccine development, as well as understanding protection and cross-protection. Despite ongoing research, significant knowledge gaps remain in the areas of neonatal and mucosal immunity. The impact of maternally derived antibody upon the neonate's ability to respond to FMD vaccination has received some attention, but few firm conclusions can be drawn at this stage, and little is known of the cellular response of young animals in general. The mucosal immune system of FMDV-susceptible species requires continued characterization, especially if the potential of mucosal vaccine-delivery systems is to be realized for FMD immunization. C1 [Robinson, L.] Insight Editing London, London, England. [Knight-Jones, T. J. D.] Int Livestock Res Inst, Lusaka, Zambia. [Charleston, B.] Pirbright Inst, Pirbright, England. [Rodriguez, L. L.] ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY USA. [Gay, C. G.] ARS, USDA, Natl Program Anim Hlth 103, Beltsville, MD USA. [Sumption, K. J.] European Commiss Control FMD EuFMD, FAO, Rome, Italy. [Vosloo, W.] CSIRO Biosecur Flagship, Australian Anim Hlth Lab, Geelong, Vic, Australia. RP Knight-Jones, TJD (reprint author), WorldFish, Int Livestock Res Inst, Lusaka, Zambia. EM t.knight-jones@cgiar.org RI Institute, Pirbright/K-4476-2014 FU EuFMD FX The authors gratefully acknowledge the input of all scientists and institutes that responded to the requests for research activity updates. The authors are also indebted to the considerable efforts of various members of the GFRA committee. Thanks also go to members of the European Commission for the Control of FMD (EuFMD) Special Committee for Research and Programme Development (SCRPD), and Jacquelyn Horsington and Nagendra Singanallur from CSIRO. This work was funded by EuFMD. NR 28 TC 0 Z9 0 U1 6 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1865-1674 EI 1865-1682 J9 TRANSBOUND EMERG DIS JI Transbound. Emerg. Dis. PD JUN PY 2016 VL 63 SU 1 SI SI BP 56 EP 62 DI 10.1111/tbed.12518 PG 7 WC Infectious Diseases; Veterinary Sciences SC Infectious Diseases; Veterinary Sciences GA DR5KY UT WOS:000379943100006 PM 27320167 ER PT J AU Robinson, L Knight-Jones, TJD Charleston, B Rodriguez, LL Gay, CG Sumption, KJ Vosloo, W AF Robinson, L. Knight-Jones, T. J. D. Charleston, B. Rodriguez, L. L. Gay, C. G. Sumption, K. J. Vosloo, W. TI Global Foot-and-Mouth Disease Research Update and Gap Analysis: 7-Pathogenesis and Molecular Biology SO TRANSBOUNDARY AND EMERGING DISEASES LA English DT Article DE foot-and-mouth disease; FMD; Pathogenesis; molecular biology; gap analysis ID SIMULATED-NATURAL INOCULATION; NONSTRUCTURAL PROTEIN 3A; LEADER PROTEINASE; VIRAL VIRULENCE; VIRUS VIRULENCE; AFRICAN BUFFALO; SEROTYPE O; INFECTION; CATTLE; CELLS AB We assessed research knowledge gaps in the fields of FMDV (foot-and-mouth disease virus) pathogenesis and molecular biology by performing a literature review (2011-15) and collecting research updates (2014) from 33 institutes from across the world. Findings were used to identify priority areas for future research. There have been important advances in FMDV pathogenesis; FMDV remains in lymph nodes of many recovered animals that otherwise do not appear persistently infected, even in species previously not associated with the carrier state. Whether virus retention helps maintain host immunity and/or virus survival is not known. Studies of FMDV pathogenesis in wildlife have provided insights into disease epidemiology, in endemic and epidemic settings. Many aspects of FMDV infection and virus entry remain unknown; however, at the cellular level, we know that expression level and availability of integrins (that permit viral entry), rate of clearance of infected cells and strength of anti-viral type I IFN (interferon) response are key determinants of tissue tropism. Extending findings to improved understanding of transmission requires a standardized approach and adoption of natural routes of infection during experimental study. There has been recognition of the importance of autophagosomes for FMDV entry into the cytoplasm following cell surface receptor binding, and that distinct internal cellular membranes are exploited for viral replication and immune evasion. New roles for viral proteins in blocking type I IFN production and downstream signalling have been identified facilitating research in anti-viral therapeutics. We know more about how infection affects cell protein expression, and research into molecular determinants of capsid stability has aided the development of stable vaccines. We have an expanding knowledge of viral and host molecular determinates of virulence and infectiousness, and of how phylogenetics may be used to estimate vaccine match and strain distribution. With ongoing advances, these areas could translate into significantly improved disease control. C1 [Robinson, L.] Insight Editing London, London, England. [Knight-Jones, T. J. D.] Int Livestock Res Inst, Lusaka, Zambia. [Charleston, B.] Pirbright Inst, Pirbright, England. [Rodriguez, L. L.] ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY USA. [Gay, C. G.] ARS, USDA, Natl Program Anim Hlth 103, Beltsville, MD USA. [Sumption, K. J.] European Commiss Control FMD EuFMD, FAO, Rome, Italy. [Vosloo, W.] CSIRO Biosecur Flagship, Australian Anim Hlth Lab, Geelong, Vic, Australia. RP Knight-Jones, TJD (reprint author), WorldFish, Int Livestock Res Inst, Lusaka, Zambia. EM t.knight-jones@cgiar.org RI Institute, Pirbright/K-4476-2014 FU EuFMD FX The authors gratefully acknowledge the input of all scientists and institutes that responded to the requests for research activity updates. The authors are also indebted to the considerable efforts of various members of the GFRA committee. Thanks also go to members of the European commission for the control of FMD (EuFMD) Special Committee for Research and Programme Development (SCRPD), and Jacquelyn Horsington and Nagendra Singanallur from CSIRO. This work was funded by EuFMD. NR 53 TC 0 Z9 0 U1 6 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1865-1674 EI 1865-1682 J9 TRANSBOUND EMERG DIS JI Transbound. Emerg. Dis. PD JUN PY 2016 VL 63 SU 1 SI SI BP 63 EP 71 DI 10.1111/tbed.12520 PG 9 WC Infectious Diseases; Veterinary Sciences SC Infectious Diseases; Veterinary Sciences GA DR5KY UT WOS:000379943100007 PM 27320168 ER PT J AU Jestrow, B Peguero, B Jimenez, F Cinea, W Hass, M Reeve, A Meerow, AW Griffith, MP Maunder, M Francisco-Ortega, J AF Jestrow, Brett Peguero, Brigido Jimenez, Francisco Cinea, William Hass, Michael Reeve, Andrew Meerow, Alan W. Griffith, M. Patrick Maunder, Michael Francisco-Ortega, Javier TI Genetic diversity and differentiation of the Critically Endangered Hispaniolan palm Coccothrinax jimenezii MM Mejia & RG Garcia based on novel SSR markers SO BIOCHEMICAL SYSTEMATICS AND ECOLOGY LA English DT Article DE Biodiversity hotspots; Caribbean islands; West Indies; Threatened species; Conservation biology; Taxonomy and conservation; Tropical islands ID POPULATION-STRUCTURE; CONSERVATION GENETICS; ARECACEAE; SOFTWARE; BIODIVERSITY; ISLAND; DEFORESTATION; DEGRADATION; ZAMIACEAE; TAXONOMY AB Coccothrinax jimenezii M.M. Mejia & R.G. Garcia is a Critically Endangered palm species restricted to Haiti (one population near the city of Gonaives with 43 individuals) and the Dominican Republic (one population on the shores of Lago Enriquillo with 18 individuals). The species faces two major conservation challenges: (1) water level rise in the hypersaline Lago Enriquillo and (2) overexploitation of leaves for making brooms in Haiti. Six SSR microsatellite loci were used to access levels of genetic variation and the genetic structure of these two populations. Only the Gonaives site had loci that deviated from Hardy-Weinberg Equilibrium (2 loci). Both populations exhibited a relatively large number of private alleles (13 in Lago Enriquillo and 14 in Gonaives) and did not show evidence of genetic bottlenecks. Inbreeding coefficients were much larger in Gonaives (F-is = 0.232) than in Lago Enriquillo (F-is = 0.093). We detected high genetic differentiation among these sites (F-st = 0.497) suggesting that additional taxonomic studies are needed to determine if individuals from these two sites should be recognized as belonging to two different taxa. Because of taxonomic uncertainties we recommend not to translocate individuals between sites in future conservation activities involving this species. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Jestrow, Brett; Hass, Michael; Reeve, Andrew; Francisco-Ortega, Javier] Fairchild Trop Bot Garden, Kushlan Trop Biol Inst, Miami, FL 33156 USA. [Peguero, Brigido; Jimenez, Francisco] Jardin Bot Nacl, Ave Republ Argentina, Santo Domingo, Dominican Rep. [Cinea, William] Jardin Bot Cayes, Route Natl 2, Les Cayes, Haiti. [Meerow, Alan W.] USDA ARS SHRS, Natl Germplasm Repository, Miami, FL 33158 USA. [Griffith, M. Patrick] Montgomery Bot Ctr, Miami, FL 33156 USA. [Maunder, Michael; Francisco-Ortega, Javier] Florida Int Univ, Cuban Res Inst, Kimberly Green Latin Amer & Caribbean Ctr, Int Ctr Trop Bot, Miami, FL 33199 USA. [Maunder, Michael; Francisco-Ortega, Javier] Florida Int Univ, Dept Biol Sci, Miami, FL 33199 USA. RP Francisco-Ortega, J (reprint author), Fairchild Trop Bot Garden, Kushlan Trop Biol Inst, Miami, FL 33156 USA.; Francisco-Ortega, J (reprint author), Florida Int Univ, Cuban Res Inst, Kimberly Green Latin Amer & Caribbean Ctr, Int Ctr Trop Bot, Miami, FL 33199 USA.; Francisco-Ortega, J (reprint author), Florida Int Univ, Dept Biol Sci, Miami, FL 33199 USA. EM ortegaj@fiu.edu FU Mohamed Bin Zayed Species Conservation Fund [through Florida International University (Flu)] [13257600]; Fairchild Tropical Botanic Garden; Consejo Nacional de Investigaciones Agropecuarias y Forestales from the Dominican Republic [CAD/014-05/RN]; Tropical Biology Program of FIU [318] FX We dedicate this paper to our colleague Walter Judd for his contributions to the flora of Haiti and his mentorship in plant systematics to a whole generation of plant taxonomists. This study was supported by the Mohamed Bin Zayed Species Conservation Fund [grant number 13257600 through Florida International University (Flu)]. Fairchild Tropical Botanic Garden provided matching funds to conduct molecular research. The Consejo Nacional de Investigaciones Agropecuarias y Forestales from the Dominican Republic supported conservation assessment studies of Critically Endangered palms (project number CAD/014-05/RN through the Consorcio Ambiental Dominicano and the Jardin Botanic Nacional). This is contribution number 318 from the Tropical Biology Program of FIU. Celio Moya and S. Zona critically read an early version of the manuscript. NR 76 TC 0 Z9 0 U1 4 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0305-1978 EI 1873-2925 J9 BIOCHEM SYST ECOL JI Biochem. Syst. Ecol. PD JUN PY 2016 VL 66 BP 216 EP 223 DI 10.1016/j.bse.2016.04.013 PG 8 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA DQ5YN UT WOS:000379281300031 ER PT J AU Bickhart, DM Xu, LY Hutchison, JL Cole, JB Null, DJ Schroeder, SG Song, JZ Garcia, JF Sonstegard, TS Van Tassell, CP Schnabel, RD Taylor, JF Lewin, HA Liu, GE AF Bickhart, Derek M. Xu, Lingyang Hutchison, Jana L. Cole, John B. Null, Daniel J. Schroeder, Steven G. Song, Jiuzhou Garcia, Jose Fernando Sonstegard, Tad S. Van Tassell, Curtis P. Schnabel, Robert D. Taylor, Jeremy F. Lewin, Harris A. Liu, George E. TI Diversity and population-genetic properties of copy number variations and multicopy genes in cattle SO DNA RESEARCH LA English DT Article DE cattle genome; population sequencing; copy number variation; taurine; indicine ID COMMON DELETION POLYMORPHISMS; MULTILOCUS GENOTYPE DATA; HUMAN GENOME; BOS-TAURUS; SEGMENTAL DUPLICATIONS; STRUCTURAL VARIATION; EUROPEAN CATTLE; SCALE VARIATION; COMPLEX TRAITS; BOVINE GENOME AB The diversity and population genetics of copy number variation (CNV) in domesticated animals are not well understood. In this study, we analysed 75 genomes of major taurine and indicine cattle breeds (including Angus, Brahman, Gir, Holstein, Jersey, Limousin, Nelore, and Romagnola), sequenced to 11-fold coverage to identify 1,853 non-redundant CNV regions. Supported by high validation rates in array comparative genomic hybridization (CGH) and qPCR experiments, these CNV regions accounted for 3.1% (87.5 Mb) of the cattle reference genome, representing a significant increase over previous estimates of the area of the genome that is copy number variable (similar to 2%). Further population genetics and evolutionary genomics analyses based on these CNVs revealed the population structures of the cattle taurine and indicine breeds and uncovered potential diversely selected CNVs near important functional genes, including AOX1, ASZ1, GAT, GLYAT, and KRTAP9-1. Additionally, 121 CNV gene regions were found to be either breed specific or differentially variable across breeds, such as RICTOR in dairy breeds and PNPLA3 in beef breeds. In contrast, clusters of the PRP and PAG genes were found to be duplicated in all sequenced animals, suggesting that subfunctionalization, neofunctionalization, or overdominance play roles in diversifying those fertility-related genes. These CNV results provide a new glimpse into the diverse selection histories of cattle breeds and a basis for correlating structural variation with complex traits in the future. C1 [Bickhart, Derek M.; Xu, Lingyang; Hutchison, Jana L.; Cole, John B.; Null, Daniel J.; Schroeder, Steven G.; Sonstegard, Tad S.; Van Tassell, Curtis P.; Liu, George E.] USDA ARS, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. [Xu, Lingyang; Song, Jiuzhou] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. [Garcia, Jose Fernando] Univ Estadual Paulista, UNESP, Aracatuba, SP, Brazil. [Schnabel, Robert D.; Taylor, Jeremy F.] Univ Missouri, Div Anim Sci, Columbia, MO 65211 USA. [Schnabel, Robert D.] Univ Missouri, Inst Informat, Columbia, MO USA. [Lewin, Harris A.] Univ Calif Davis, Dept Ecol & Evolut, Davis, CA 95616 USA. RP Bickhart, DM; Liu, GE (reprint author), USDA ARS, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. EM derek.bickhart@ars.usda.gov; george.liu@ars.usda.gov RI Cole, John/J-8571-2014; Frolov, Dmitry/B-6512-2016; OI Cole, John/0000-0003-1242-4401; Frolov, Dmitry/0000-0002-9166-1132; Schroeder, Steven/0000-0001-9103-5150; Bickhart, Derek/0000-0003-2223-9285 FU Agriculture and Food Research Initiative (AFRI) competitive grant from the USDA National Institute of Food and Agriculture (NIFA) Animal Genome Program [2011-67015-30183]; AFRI competitive grant from the USDA NIFA [2009-65205-05635]; AFRI competitive grant from the USDA NIFA Animal Genome Program [2011-68004-30367, 2011-68004-30214, 2013-68004-20364] FX This work was supported in part by Agriculture and Food Research Initiative (AFRI) competitive grant No. 2011-67015-30183 from the USDA National Institute of Food and Agriculture (NIFA) Animal Genome Program. J.F.T. and C.P.V.T. were supported by AFRI competitive grant No. 2009-65205-05635 from the USDA NIFA. J.F.T. and R.D.S. were further supported by AFRI competitive grants No. 2011-68004-30367, 2011-68004-30214 and 2013-68004-20364 from the USDA NIFA Animal Genome Program. Funding to pay the Open Access publication charges for this article was provided by the USDA National Institute of Food and Agriculture (NIFA) Animal Genome Program. NR 75 TC 0 Z9 0 U1 6 U2 8 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1340-2838 EI 1756-1663 J9 DNA RES JI DNA Res. PD JUN PY 2016 VL 23 IS 3 BP 253 EP 262 DI 10.1093/dnares/dsw013 PG 10 WC Genetics & Heredity SC Genetics & Heredity GA DR2NP UT WOS:000379741500007 PM 27085184 ER PT J AU Manoli, G Domec, JC Novick, K Oishi, AC Noormets, A Marani, M Katul, G AF Manoli, Gabriele Domec, Jean-Christophe Novick, Kimberly Oishi, Andrew Christopher Noormets, Asko Marani, Marco Katul, Gabriel TI Soil-plant-atmosphere conditions regulating convective cloud formation above southeastern US pine plantations SO GLOBAL CHANGE BIOLOGY LA English DT Article DE convective clouds; forest ecosystem; land-cover change; soil-plant-atmosphere interactions ID NET ECOSYSTEM EXCHANGE; BOUNDARY-LAYER CLOUDS; UNITED-STATES; STOMATAL CONDUCTANCE; MOISTURE FEEDBACKS; CLIMATE-CHANGE; LOBLOLLY-PINE; LAND-SURFACE; WATER-USE; FOREST AB Loblolly pine trees (Pinus taeda L.) occupy more than 20% of the forested area in the southern United States, represent more than 50% of the standing pine volume in this region, and remove from the atmosphere about 500 g C m(-2) per year through net ecosystem exchange. Hence, their significance as a major regional carbon sink can hardly be disputed. What is disputed is whether the proliferation of young plantations replacing old forest in the southern United States will alter key aspects of the hydrologic cycle, including convective rainfall, which is the focus of the present work. Ecosystem fluxes of sensible (H-s) and latent heat (LE) and large-scale, slowly evolving free atmospheric temperature and water vapor content are known to be first-order controls on the formation of convective clouds in the atmospheric boundary layer. These controlling processes are here described by a zero-order analytical model aimed at assessing how plantations of different ages may regulate the persistence and transition of the atmospheric system between cloudy and cloudless conditions. Using the analytical model together with field observations, the roles of ecosystem H-s and LE on convective cloud formation are explored relative to the entrainment of heat and moisture from the free atmosphere. Our results demonstrate that cloudy-cloudless regimes at the land surface are regulated by a nonlinear relation between the Bowen ratio Bo = H-s/LE and root-zone soil water content, suggesting that young/mature pines ecosystems have the ability to recirculate available water (through rainfall predisposition mechanisms). Such nonlinearity was not detected in a much older pine stand, suggesting a higher tolerance to drought but a limited control on boundary layer dynamics. These results enable the generation of hypotheses about the impacts on convective cloud formation driven by afforestation/deforestation and groundwater depletion projected to increase following increased human population in the southeastern United States. C1 [Manoli, Gabriele; Domec, Jean-Christophe; Marani, Marco; Katul, Gabriel] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Domec, Jean-Christophe; Noormets, Asko] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27607 USA. [Novick, Kimberly] Indiana Univ, Sch Publ & Environm Affairs, Bloomington, IN 47405 USA. [Oishi, Andrew Christopher] US Forest Serv, Coweeta Hydrol Lab, USDA, Otto, NC 28763 USA. RP Manoli, G (reprint author), Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. EM gabriele.manoli@duke.edu RI Katul, Gabriel/A-7210-2008; Marani, Marco/F-9451-2016; OI Katul, Gabriel/0000-0001-9768-3693; Marani, Marco/0000-0002-1493-6913; Manoli, Gabriele/0000-0002-9245-2877 FU National Science Foundation [NSF-EAR-1344703]; US Department of Energy through the Office of Biological and Environmental Research Terrestrial Carbon Processes program [DE-SC0006967, DE-SC0011461]; DOE BER-TES [7090112, 11-DE-SC-0006700]; USDA NEFA CAP Award [2011-68002-30185]; USDA Forest Service Eastern Forest Environmental Threat Assessment Center Grant [08-JV-11330147-038] FX This study was supported by the National Science Foundation (NSF-EAR-1344703) and the US Department of Energy through the Office of Biological and Environmental Research Terrestrial Carbon Processes program (DE-SC0006967 and DE-SC0011461). US-Net and US-NC2 flux sites have been supported by DOE BER-TES awards number 7090112 and 11-DE-SC-0006700, USDA NEFA CAP Award 2011-68002-30185, and USDA Forest Service Eastern Forest Environmental Threat Assessment Center Grant 08-JV-11330147-038. The authors confirm that they have no interest or relationship, financial, or otherwise that might be perceived as influencing objectivity with respect to this work. NR 74 TC 2 Z9 2 U1 6 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD JUN PY 2016 VL 22 IS 6 BP 2238 EP 2254 DI 10.1111/gcb.13221 PG 17 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DP8BA UT WOS:000378721700019 PM 26762609 ER PT J AU Zhang, MQ Guo, Y Powell, CA Doud, MS Yang, CY Zhou, H Duan, YP AF Zhang, M. Q. Guo, Y. Powell, C. A. Doud, M. S. Yang, C. Y. Zhou, H. Duan, Y. P. TI Zinc treatment increases the titre of 'Candidatus Liberibacter asiaticus' in huanglongbing-affected citrus plants while affecting the bacterial microbiomes SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE Candidatus Liberibacter asiaticus; citrus huanglongbing; microbial community; Micronutrient zinc; PhyloChip ID STENOTROPHOMONAS-MALTOPHILIA; INTERACTIVE TREE; INFECTED CITRUS; HEAVY-METALS; GROWTH; TOLERANCE; DIVERSITY; SOIL; DISEASE; SIDEROPHORES AB Aims: Huanglongbing (HLB)-affected citrus often display zinc deficiency symptoms. In this study, supplemental zinc was applied to citrus to determine its effect on Candidatus Liberibacter asiaticus (Las) titre, HLB symptoms, and leaf microbiome. Methods and Results: HLB-affected citrus were treated with various amounts of zinc. The treatments promoted Las growth and affected microbiomes in citrus leaves. Phylochip(TM) - based results indicated that 5475 of over 50 000 known Operational Taxonomic Units (OTUs) in 52 phyla were detected in the midribs of HLB-affected citrus, of which Proteobacteria was the most abundant, followed by Firmicutes and Actinobacteria. In comparison, the microbiomes of zinc-treated diseased plants had overall more OTUs with higher amounts of Proteobacteria, but decreased percentages of Firmicutes and Actinobacteria. In addition, more OTUs of siderophore-producing bacteria were present. Only zinc-sensitive Staphylococcaceae had higher OTU's in the diseased plants without zinc treatments. Conclusions: Although HLB-affected citrus appear zinc deficient, zinc amendments increased the pathogen levels and shifted the microbiome. Significance and Impact of the Study: HLB is currently the most devastating disease of citrus worldwide. Zinc is often applied to HLB-affected citrus due to zinc deficiency symptoms. This study provided new insights into the potential effects of zinc on HLB and the microbial ecology of citrus. C1 [Zhang, M. Q.; Zhou, H.] Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning, Peoples R China. [Zhang, M. Q.; Guo, Y.; Powell, C. A.; Yang, C. Y.] Univ Florida, IRREC IFAS, Ft Pierce, FL USA. [Zhang, M. Q.; Doud, M. S.; Duan, Y. P.] USDA ARS, USHRL, 2001 S Rock Rd, Ft Pierce, FL 34945 USA. RP Duan, YP (reprint author), USDA ARS, USHRL, 2001 S Rock Rd, Ft Pierce, FL 34945 USA. EM Yongping.Duan@usda.ars.gov FU Citrus Research and Development Foundation awards (CRDF); Citrus Research and Development Foundation awards (CRDF) [410] FX This study was supported by Citrus Research and Development Foundation awards (CRDF#410). We thank Ms. Christina Latza for her excellent technical assistance in this research. NR 64 TC 0 Z9 1 U1 13 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 EI 1365-2672 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD JUN PY 2016 VL 120 IS 6 BP 1616 EP 1628 DI 10.1111/jam.13102 PG 13 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DQ9MU UT WOS:000379534800016 PM 26909469 ER PT J AU Hobbins, MT Wood, A McEvoy, DJ Huntington, JL Morton, C Anderson, M Hain, C AF Hobbins, Michael T. Wood, Andrew McEvoy, Daniel J. Huntington, Justin L. Morton, Charles Anderson, Martha Hain, Christopher TI The Evaporative Demand Drought Index. Part I: Linking Drought Evolution to Variations in Evaporative Demand SO JOURNAL OF HYDROMETEOROLOGY LA English DT Article ID SOIL-MOISTURE; UNITED-STATES; REFERENCE EVAPOTRANSPIRATION; SEVERITY INDEX; MODEL; PRECIPITATION; VARIABILITY; FORECASTS AB Many operational drought indices focus primarily on precipitation and temperature when depicting hydroclimatic anomalies, and this perspective can be augmented by analyses and products that reflect the evaporative dynamics of drought. The linkage between atmospheric evaporative demand E-0 and actual evapotranspiration (ET) is leveraged in a new drought index based solely on E-0-the Evaporative Demand Drought Index (EDDI). EDDI measures the signal of drought through the response of E-0 to surface drying anomalies that result from two distinct land surface-atmosphere interactions: 1) a complementary relationship between E-0 and ET that develops under moisture limitations at the land surface, leading to ET declining and increasing E-0, as in sustained droughts, and 2) parallel ET and E-0 increases arising from increased energy availability that lead to surface moisture limitations, as in flash droughts. To calculate EDDI from E-0, a long-term, daily reanalysis of reference ET estimated from the American Society of Civil Engineers (ASCE) standardized reference ET equation using radiation and meteorological variables from the North American Land Data Assimilation System phase 2 (NLDAS-2) is used. EDDI is obtained by deriving empirical probabilities of aggregated E-0 depths relative to their climatologic means across a user-specific time period and normalizing these probabilities. Positive EDDI values then indicate drier-than-normal conditions and the potential for drought. EDDI is a physically based, multiscalar drought index that that can serve as an indicator of both flash and sustained droughts, in some hydroclimates offering early warning relative to current operational drought indices. The performance of EDDI is assessed against other commonly used drought metrics across CONUS in Part II. C1 [Hobbins, Michael T.] Univ Colorado, Cooperat Inst Res Environm Sci, Boulder, CO 80309 USA. [Hobbins, Michael T.] NOAA, Earth Syst Res Lab, Div Phys Sci, 325 Broadway, Boulder, CO 80305 USA. [Wood, Andrew] Natl Ctr Atmospher Res, POB 3000, Boulder, CO 80307 USA. [McEvoy, Daniel J.; Huntington, Justin L.; Morton, Charles] Desert Res Inst, Reno, NV USA. [Anderson, Martha] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD USA. [Hain, Christopher] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20742 USA. RP Hobbins, MT (reprint author), NOAA, Earth Syst Res Lab, Div Phys Sci, 325 Broadway, Boulder, CO 80305 USA. EM mike.hobbins@noaa.gov OI Anderson, Martha/0000-0003-0748-5525 FU National Integrated Drought Information System (NIDIS); U.S. Agency for International Development (USAID); NOAA [AID-FFP-P-10-00002/006]; NOAA MAPP Grant [NA11OAR4310142]; DRI Maki Endowment [6223-640-0969]; U.S. Bureau of Reclamation Climate Analysis Tools WaterSMART Program Grant [R11AP81454]; U.S. Geological Survey; DRI Great Basin Cooperative Ecosystem Study Unit Collaborative Project on Drought Monitoring and Fallow Field-Tracking through Cloud Computing of Landsat; MODIS; Gridded Climate Data Archives Grant [G15AC00137]; U.S. Bureau of Land Management Grant [L13AC00169]; NASA Applied Sciences Water Resources Grant [NNX12AK90G] FX The NLDAS-2 data were acquired as part of the mission of NASA's Earth Science Division and archived and distributed by the Goddard Earth Sciences (GES) Data and Information Services Center (DISC). Dr. Hobbins was supported by the National Integrated Drought Information System (NIDIS) and from an Inter-Agency Agreement between the U.S. Agency for International Development (USAID) and NOAA for support to the Famine Early Warning Systems Network (FEWS NET; AID-FFP-P-10-00002/006). Dr. Wood was supported by a NOAA MAPP Grant (NA11OAR4310142). Dr. McEvoy, Dr. Huntington, and Mr. Morton were supported by a DRI Maki Endowment for enhancing water resource monitoring in Southern Nevada Grant (6223-640-0969); a U.S. Bureau of Reclamation Climate Analysis Tools WaterSMART Program Grant (R11AP81454); a U.S. Geological Survey and DRI Great Basin Cooperative Ecosystem Study Unit Collaborative Project on Drought Monitoring and Fallow Field-Tracking through Cloud Computing of Landsat, MODIS, and Gridded Climate Data Archives Grant (G15AC00137); and a U.S. Bureau of Land Management Grant (L13AC00169). Dr. Anderson and Dr. Hain were supported by a NASA Applied Sciences Water Resources Grant (NNX12AK90G). NR 43 TC 5 Z9 5 U1 10 U2 13 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 1525-755X EI 1525-7541 J9 J HYDROMETEOROL JI J. Hydrometeorol. PD JUN PY 2016 VL 17 IS 6 BP 1745 EP 1761 DI 10.1175/JHM-D-15-0121.1 PG 17 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DQ9CK UT WOS:000379507800004 ER PT J AU McEvoy, DJ Huntington, JL Hobbins, MT Wood, A Morton, C Anderson, M Hain, C AF McEvoy, Daniel J. Huntington, Justin L. Hobbins, Michael T. Wood, Andrew Morton, Charles Anderson, Martha Hain, Christopher TI The Evaporative Demand Drought Index. Part II: CONUS-Wide Assessment against Common Drought Indicators SO JOURNAL OF HYDROMETEOROLOGY LA English DT Article ID CONTERMINOUS UNITED-STATES; REFERENCE EVAPOTRANSPIRATION; ATMOSPHERIC RIVERS; STRESS INDEX; WEST-COAST; PRECIPITATION; VARIABILITY; MODEL; FRAMEWORK; MONITOR AB Precipitation, soil moisture, and air temperature are the most commonly used climate variables to monitor drought; however, other climatic factors such as solar radiation, wind speed, and humidity can be important drivers in the depletion of soil moisture and evolution and persistence of drought. This work assesses the Evaporative Demand Drought Index (EDDI) at multiple time scales for several hydroclimates as the second part of a two-part study. EDDI and individual evaporative demand components were examined as they relate to the dynamic evolution of flash drought over the central United States, characterization of hydrologic drought over the western United States, and comparison to commonly used drought metrics of the U.S. Drought Monitor (USDM), Standardized Precipitation Index (SPI), Standardized Soil Moisture Index (SSI), and the evaporative stress index (ESI). Two main advantages of EDDI over other drought indices are that it is independent of precipitation (similar to ESI) and it can be decomposed to identify the role individual evaporative drivers have on drought onset and persistence. At short time scales, spatial distributions and time series results illustrate that EDDI often indicates drought onset well in advance of the USDM, SPI, and SSI. Results illustrate the benefits of physically based evaporative demand estimates and demonstrate EDDI's utility and effectiveness in an easy-to-implement agricultural early warning and long-term hydrologic drought-monitoring tool with potential applications in seasonal forecasting and fire-weather monitoring. C1 [McEvoy, Daniel J.; Huntington, Justin L.; Morton, Charles] Desert Res Inst, Reno, NV 89512 USA. [Hobbins, Michael T.] Univ Colorado, Cooperat Inst Res Environm Sci, Boulder, CO 80309 USA. [Hobbins, Michael T.] NOAA, Earth Syst Res Lab, Div Phys Sci, Boulder, CO USA. [Wood, Andrew] Natl Ctr Atmospher Res, POB 3000, Boulder, CO 80307 USA. [Anderson, Martha] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD USA. [Hain, Christopher] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20742 USA. RP McEvoy, DJ (reprint author), Desert Res Inst, Western Reg Climate Ctr, 2215 Raggio Pkwy, Reno, NV 89512 USA. EM mcevoyd@dri.edu OI Anderson, Martha/0000-0003-0748-5525 FU National Integrated Drought Information System (NIDIS); U.S. Agency for International Development (USAID); NOAA [AID-FFP-P-10-00002/006]; NOAA MAPP Grant [NA11OAR4310142]; DRI Maki Endowment for Enhancing Water Resource Monitoring in Southern Nevada Grant [6223-640-0969]; U.S. Bureau of Reclamation Climate Analysis Tools WaterSMART Program Grant [R11AP81454]; U.S. Geological Survey; DRI Great Basin Cooperative Ecosystem Study Unit Collaborative Project on Drought Monitoring and Fallow Field-Tracking through Cloud Computing of Landsat, MODIS; Gridded Climate Data Archives Grant [G15AC00137]; U.S. Bureau of Land Management Grant [L13AC00169]; NASA Applied Sciences Water Resources Grant [NNX12AK90G] FX The NLDAS-2 data were acquired as part of the mission of NASA's Earth Science Division and archived and distributed by the Goddard Earth Sciences (GES) Data and Information Services Center (DISC). Dr. Hobbins was supported from the National Integrated Drought Information System (NIDIS) and from an Inter-Agency Agreement between the U.S. Agency for International Development (USAID) and NOAA for support to the Famine Early Warning Systems Network (FEWS NET; AID-FFP-P-10-00002/006). Dr. Wood was supported by a NOAA MAPP Grant (NA11OAR4310142). Dr. McEvoy, Dr. Huntington, and Mr. Morton were supported by a DRI Maki Endowment for Enhancing Water Resource Monitoring in Southern Nevada Grant (6223-640-0969), a U.S. Bureau of Reclamation Climate Analysis Tools WaterSMART Program Grant (R11AP81454), a U.S. Geological Survey and DRI Great Basin Cooperative Ecosystem Study Unit Collaborative Project on Drought Monitoring and Fallow Field-Tracking through Cloud Computing of Landsat, MODIS, and Gridded Climate Data Archives Grant (G15AC00137), and a U.S. Bureau of Land Management Grant (L13AC00169). Dr. Anderson and Dr. Hain were supported by a NASA Applied Sciences Water Resources Grant (NNX12AK90G). NR 57 TC 4 Z9 4 U1 9 U2 12 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 1525-755X EI 1525-7541 J9 J HYDROMETEOROL JI J. Hydrometeorol. PD JUN PY 2016 VL 17 IS 6 BP 1763 EP 1779 DI 10.1175/JHM-D-15-0122.1 PG 17 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DQ9CK UT WOS:000379507800005 ER PT J AU Morris, KA Langston, DB Davis, RF Noe, JP Dickson, DW Timper, P AF Morris, Kelly A. Langston, David B. Davis, Richard F. Noe, James P. Dickson, Don W. Timper, Patricia TI Efficacy of Various Application Methods of Fluensulfone for Managing Root-knot Nematodes in Vegetables SO JOURNAL OF NEMATOLOGY LA English DT Article DE cucumber; fluensulfone; management; Meloidogyne spp.; nematicide; oxamyl; tomato; vegetable crops ID MELOIDOGYNE-INCOGNITA; FOLIAR APPLICATIONS; NEMATICIDE OXAMYL; INHIBITION; TOMATO; HAPLA; PESTICIDES; MANAGEMENT; CARBAMATE; ALDICARB AB Fluensulfone is a new nematicide in the flouroalkenyl chemical group. A field experiment was conducted in 2012 and 2013 to evaluate the efficacy of various application methods of fluensulfone for control of Meloidogyne spp. in cucumber (Cucumis sativus). Treatments of fluensulfone (3.0 kg a.i./ha) were applied either as preplant incorporation (PPI) or via different drip irrigation methods: drip without pulse irrigation (Drip NP), pulse irrigation 1 hr after treatment (Drip +1P), and treatment at the same time as pulse irrigation (Drip =P). The experiment had eight replications per treatment and also included a PPI treatment of oxamyl (22.5 kg a.i./ha) and a nontreated control. Compared to the control, neither the oxamyl nor the fluensulfone PPI treatments reduced root galling by Meloidogyne spp. in cucumber. Among the drip treatments, Drip NP and Drip +1P reduced root galling compared to the control. Cucumber yield was greater in all fluensulfone treatments than in the control. In a growth-chamber experiment, the systemic activity and phytotoxicity of fluensulfone were also evaluated on tomato (Solanum lycopersicum), eggplant (Solanum melongena), cucumber, and squash (Curcurbita pepo). At the seedling stage, foliage of each crop was sprayed with fluensulfone at 3, 6, and 12 g a.i./liter, oxamyl at 4.8 g a.i./liter, or water (nontreated control). Each plant was inoculated with Meloidogyne incognita juveniles 2 d after treatment. There were six replications per treatment and the experiment was conducted twice. Foliar applications of fluensulfone reduced plant vigor and dry weight of eggplant and tomato, but not cucumber or squash; application of oxamyl had no effect on the vigor or weight of any of the crops. Typically, only the highest rate of fluensulfone was phytotoxic to eggplant and tomato. Tomato was the only crop tested in which there was a reduction in the number of nematodes or galls when fluensulfone or oxamyl was applied to the foliage compared to the nontreated control. This study demonstrates that control of Meloidogyne spp. may be obtained by drip and foliar applications of fluensulfone; however, the systemic activity of fluensulfone is crop specific and there is a risk of phytotoxicity with foliar applications. C1 [Morris, Kelly A.; Langston, David B.] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. [Davis, Richard F.; Timper, Patricia] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. [Noe, James P.] Univ Georgia, Dept Plant Pathol, Athens, GA 30602 USA. [Dickson, Don W.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Langston, David B.] Tidewater Agr Res & Extens Ctr, 6321 Holland Rd, Suffolk, VA 23437 USA. RP Timper, P (reprint author), USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. EM patricia.timper@ars.usda.gov NR 33 TC 0 Z9 0 U1 7 U2 7 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD JUN PY 2016 VL 48 IS 2 BP 65 EP 71 PG 7 WC Zoology SC Zoology GA DQ7ZC UT WOS:000379427200001 PM 27418698 ER PT J AU Leite, LG Shapiro-Ilan, DI Hazir, S Jackson, MA AF Leite, Luis G. Shapiro-Ilan, David I. Hazir, Selcuk Jackson, Mark A. TI The Effects of Nutrient Concentration, Addition of Thickeners, and Agitation Speed on Liquid Fermentation of Steinernema feltiae SO JOURNAL OF NEMATOLOGY LA English DT Article DE biocontrol; entomopathogenic nematode; in vitro production; liquid fermentation ID ENTOMOPATHOGENIC NEMATODE PRODUCTION; BACTERIUM PHOTORHABDUS-LUMINESCENS; SUBMERGED MONOXENIC CULTURE; FREE-LIVING NEMATODE; FED-BATCH CULTURE; HETERORHABDITIS-BACTERIOPHORA; MASS-PRODUCTION; ASPERGILLUS-NIDULANS; GROWTH; NEOAPLECTANA AB Entomopathogenic nematode production in liquid fermentation still requires improvements to maximize efficiency, yield, and nematode quality. Therefore, this study was aimed at developing a more suitable liquid medium for mass production of Steinernema feltiae, by assessing the effects of nutrient concentration, thickeners (primarily agar), and agitation speed on infective juvenile (IJ) yield. Base medium (BM) contained yeast extract (2.3%), egg yolk (1.25%), NaCl (0.5%), and corn oil (4%). All media were inoculated with Xenorhabdus bovienii, and 2 d later, with 2-d-old S. feltiae juveniles. For the nutrient concentration experiment, we evaluated the base medium versus a modified base medium containing all the components, but with 3x concentrations of yeast extract (6.9%), egg yolk (3.75%), and corn oil (12%). The nematodes and bacteria were cultured in 150-ml Erlenmeyer flasks containing 50 ml of liquid medium at (25 degrees C) and 180 rpm on a rotary shaker incubator. To assess the effect of thickeners, IJs were inoculated in BM with agar (0.2%), carrageen (0.2%), and carboxymethyl cellulose (0.2% and 0.5%). The addition of 3x more nutrients relative to the BM resulted in a significantly lower yield of nematodes. For agar and agitation speed experiments, five levels of agar in the BM (0%, 0.2%, 0.4%, 0.6%, and 0.8% agar) and two agitation speeds (180 and 280 rpm) were evaluated for production. Increasing agitation speed from 180 to 280 rpm and higher levels of agar in the medium (> 0.2%) significantly increased the yield of bacteria. At the lower agitation speed, media amended with 0.4% and 0.6% agar produced higher nematode yields compared to media without agar. Media with 0.2% and 0.8% agar resulted in intermediate levels of nematode production. At the higher agitation speed, media supplemented with 0.8% agar resulted in the lowest yield of nematodes when compared to the other media tested. Results indicated that increasing nutrient concentration levels was detrimental to nematode production. Also, media containing agar (0.4% and 0.6%) increased nematode yields when cultures were grown at low agitation speed. When IJs were used as the inoculum, 0.2% agar also enhanced recovery and nematode yield at the higher agitation speed. C1 [Leite, Luis G.] APTA, Inst Biol, CP 70, BR-13001970 Campinas, SP, Brazil. [Shapiro-Ilan, David I.] USDA ARS, SEFTNRL, Byron, GA 31008 USA. [Hazir, Selcuk] Adnan Menderes Univ, Fac Arts & Sci, Dept Biol, Aydin, Turkey. [Jackson, Mark A.] USDA ARS, NCAUR, Peoria, IL 61604 USA. RP Leite, LG (reprint author), APTA, Inst Biol, CP 70, BR-13001970 Campinas, SP, Brazil. EM lgleite@biologico.sp.gov.br; david.shapiro@ars.usda.gov FU Sao Paulo Research Foundation FAPESP [2014/00651-4]; USDA National Program for Crop Production [6042-21220-012-00D] FX The authors would like to thank the Sao Paulo Research Foundation FAPESP (grant 2014/00651-4) for supporting the first author. We also thank Stacy Byrd for her valuable technical assistance. The research was partially supported by the USDA National Program for Crop Production (project number: 6042-21220-012-00D). NR 48 TC 0 Z9 0 U1 3 U2 3 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD JUN PY 2016 VL 48 IS 2 BP 126 EP 133 PG 8 WC Zoology SC Zoology GA DQ7ZC UT WOS:000379427200009 PM 27418706 ER PT J AU Meyer, SLF Chauhan, KR MacDonald, MH AF Meyer, Susan L. F. Chauhan, Kamlesh R. MacDonald, Margaret H. TI EVALUATION OF ROSELLE (HIBISCUS SABDARIFFA) LEAF AND POMEGRANATE (PUNICA GRANATUM) FRUIT RIND FOR ACTIVITY AGAINST MELOIDOGYNE INCOGNITA SO NEMATROPICA LA English DT Article DE gongura; Hibiscus sabdariffa; management; Meloidogyne incognita; natural products; plant extracts; pomegranate; Punica granatum; root-knot nematode; roselle; soil amendments ID EXTRACTS; ACID; ELLAGITANNINS; MANAGEMENT; HERBIVORY; PEEL; L. AB Pomegranate (Punica granatum) fruit and roselle (Hibiscus sabdariffa) leaves have been used in traditional medicine, including as anthelmintics. Methanolic extracts from these plants were investigated for activity against the southern root-knot nematode (RKN), Meloidogyne incognita. Dried, ground powders were prepared from pomegranate fruit rinds and from roselle leaves. In in vitro assays, methanolic extracts from both powders, dried and dissolved in water, inhibited egg hatch and viability of second-stage juveniles (J2). Some of the effect of these extracts on J2 was nematostatic, depending on the extract concentration. The pomegranate extract (pH 3.4 to 3.8) was effective at the tested concentrations of 0.45% to 1.8% (volume extract per volume water), reducing egg hatch up to 93.9%, and killing more than 30% of the J2. Roselle extract concentrations of 4.5% and higher (pH 2.8 to 3.6) suppressed egg hatch by up to 97.4%, and 100% J2 mortality occurred in concentrations of 22.5% and 45.0% extract. Pomegranate and roselle extracts with pH adjusted to higher values (5.9 and 5.0, respectively) were also active against RKN. Pomegranate extract, pH 5.9, resulted in 96.5% to 99.7% egg hatch suppression and 100% J2 mortality at concentrations of 22.5% to 33.8%. Pomegranate fruit rind powder was also tested as a soil amendment in greenhouse trials, and was phytotoxic to cucumber seedlings at application rates of 5.0% and 10.0% (weight dried pomegranate rind/weight dried soil), resulting in plant death. Shoot heights and fresh weights were reduced at 0.25%, 0.5%, and 1.0% powder application rates, and root fresh weights also tended to be lower at these concentrations. The numbers of galls per root system and galls per g root fresh weight were suppressed in the 1.0% application rate. Amendment with pomegranate fruit rind powder resulted in rapid growth of Rhizopus sp. and Aspergillus sp. on the soil. Suppression of RKN egg hatch and J2 activity by extracts from pomegranate fruit rinds and roselle leaves indicate that these plant-derived products are potential candidates for future studies of nematode-antagonistic compounds. C1 [Meyer, Susan L. F.; MacDonald, Margaret H.] ARS, USDA, Nematol Lab, Northeast Area,Henry A Wallace Beltsville Agr Res, Bldg 010A,Rm 112, Beltsville, MD 20705 USA. [Chauhan, Kamlesh R.] ARS, USDA, Invas Insect Biocontrol & Behav Lab, Northeast Area,Henry A Wallace Beltsville Agr Res, Bldg 007,Rm 303, Beltsville, MD 20705 USA. RP Meyer, SLF (reprint author), ARS, USDA, Nematol Lab, Northeast Area,Henry A Wallace Beltsville Agr Res, Bldg 010A,Rm 112, Beltsville, MD 20705 USA. EM Susan.L.Meyer@ars.usda.gov NR 31 TC 0 Z9 0 U1 4 U2 4 PU ORGANIZATION TROP AMER NEMATOLOGISTS PI AUBURN PA AUBURN UNIV DEPT PLANT PATHOLOGY, AUBURN, AL 36849 USA SN 0099-5444 EI 2220-5608 J9 NEMATROPICA JI Nematropica PD JUN PY 2016 VL 46 IS 1 BP 85 EP 96 PG 12 WC Zoology SC Zoology GA DQ6XX UT WOS:000379351200013 ER PT J AU Sikulu-Lord, MT Maia, MF Milali, MP Henry, M Mkandawile, G Kho, EA Wirtz, RA Hugo, LE Dowell, FE Devine, GJ AF Sikulu-Lord, Maggy T. Maia, Marta F. Milali, Masabho P. Henry, Michael Mkandawile, Gustav Kho, Elise A. Wirtz, Robert A. Hugo, Leon E. Dowell, Floyd E. Devine, Gregor J. TI Rapid and Non-destructive Detection and Identification of Two Strains of Wolbachia in Aedes aegypti by Near-Infrared Spectroscopy SO PLOS NEGLECTED TROPICAL DISEASES LA English DT Article ID LIFE-SHORTENING WOLBACHIA; CYTOPLASMIC INCOMPATIBILITY; DENGUE TRANSMISSION; BLOCKS DENGUE; AGE; INFECTION; DROSOPHILA; MOSQUITOS; VIRUS; PCR AB The release of Wolbachia infected mosquitoes is likely to form a key component of disease control strategies in the near future. We investigated the potential of using near-infrared spectroscopy (NIRS) to simultaneously detect and identify two strains of Wolbachia pipientis (wMelPop and wMel) in male and female laboratory-reared Aedes aegypti mosquitoes. Our aim is to find faster, cheaper alternatives for monitoring those releases than the molecular diagnostic techniques that are currently in use. Our findings indicate that NIRS can differentiate females and males infected with wMelPop from uninfected wild type samples with an accuracy of 96% (N = 299) and 87.5% (N = 377), respectively. Similarly, females and males infected with wMel were differentiated from uninfected wild type samples with accuracies of 92% (N = 352) and 89% (N = 444). NIRS could differentiate wMelPop and wMel transinfected females with an accuracy of 96.6% (N = 442) and males with an accuracy of 84.5% (N = 443). This non-destructive technique is faster than the standard polymerase chain reaction diagnostic techniques. After the purchase of a NIRS spectrometer, the technique requires little sample processing and does not consume any reagents. C1 [Sikulu-Lord, Maggy T.; Kho, Elise A.; Hugo, Leon E.; Devine, Gregor J.] QIMR Berghofer Med Res Inst, Mosquito Control Lab, Brisbane, Qld, Australia. [Sikulu-Lord, Maggy T.; Maia, Marta F.; Milali, Masabho P.; Henry, Michael; Mkandawile, Gustav] Ifakara Hlth Inst, Environm Hlth & Ecol Sci Themat Grp, Ifakara, Tanzania. [Wirtz, Robert A.] Ctr Dis Control & Prevent, Atlanta, GA USA. [Dowell, Floyd E.] USDA ARS, Stored Prod Insect & Engn Res Unit, Ctr Grain & Anim Hlth Res, Manhattan, KS USA. RP Sikulu-Lord, MT (reprint author), QIMR Berghofer Med Res Inst, Mosquito Control Lab, Brisbane, Qld, Australia.; Sikulu-Lord, MT (reprint author), Ifakara Hlth Inst, Environm Hlth & Ecol Sci Themat Grp, Ifakara, Tanzania. EM maggy.sikulu@qimrberghofer.edu.au FU Grand Challenges Canada Stars for Global Health - Government of Canada [0439-001]; Mosquito and Arbovirus Research Committee, Queensland, Australia FX This study was supported by Grand Challenges Canada Stars for Global Health funded by the Government of Canada (grant 0439-001) awarded to MTSL and the Mosquito and Arbovirus Research Committee, Queensland, Australia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 55 TC 1 Z9 1 U1 10 U2 12 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1935-2735 J9 PLOS NEGLECT TROP D JI Plos Neglect. Trop. Dis. PD JUN PY 2016 VL 10 IS 6 AR e0004759 DI 10.1371/journal.pntd.0004759 PG 12 WC Infectious Diseases; Parasitology; Tropical Medicine SC Infectious Diseases; Parasitology; Tropical Medicine GA DQ6WL UT WOS:000379346200025 PM 27362709 ER PT J AU Redwood, ME Matlack, GR Huebner, CD AF Redwood, Mame E. Matlack, Glenn R. Huebner, Cynthia D. TI Seed longevity and dormancy state in a disturbance-dependent forest herb, Ageratina altissima SO SEED SCIENCE RESEARCH LA English DT Article DE Ageratina; bet-hedging; dispersal; environmental heterogeneity; Eupatorium; germination; induced dormancy ID TEMPERATE DECIDUOUS FOREST; VARIABLE ENVIRONMENTS; MICROSTEGIUM-VIMINEUM; EUPATORIUM-RUGOSUM; DISPERSAL; GERMINATION; BANKS; SIZE; STRATEGIES; ASTERACEAE AB Does seed dormancy allow disturbance-oriented forest herbs such as Ageratina altissima to persist in heterogeneous natural communities? To document seed longevity and dormancy state, Ageratina seeds were buried in nylon mesh bags in second-growth forest stands in south-eastern Ohio, USA. Bags were recovered at 2-month intervals, and seeds were tested for viability and germinability in the lab. Live seed numbers declined rapidly, with seed banks exhausted in an estimated 33-37 months. Seeds showed a strong dormancy polymorphism, with 71-84% of live seeds germinable between March and July, the season of natural seedling emergence. At other dates, most seeds appeared to be in a state of induced dormancy, allowing little (0-21%) germination. The slope aspect of the burial site, an important factor controlling above-ground vegetation, had no effect on seed longevity or dormancy condition. Dormancy in Ageratina appears to be adapted to allow opportunistic germination in late spring-early summer, but to prevent germination in less benign periods. Seed longevity is shorter than the natural frequency of gaps in mesophytic forest. We conclude that a long-term dispersal/dormancy trade-off is probably an oversimplification of the spatial ecology of this species. The primary function of dormancy appears to be short-term tracking of seasonal variation. C1 [Redwood, Mame E.; Matlack, Glenn R.] Ohio Univ, Environm & Plant Biol, Porter Hall, Athens, OH 45701 USA. [Huebner, Cynthia D.] USDA Forest Serv, No Res Stn, Morgantown, WV USA. RP Matlack, GR (reprint author), Ohio Univ, Environm & Plant Biol, Porter Hall, Athens, OH 45701 USA. EM Matlack@ohio.edu FU USDA Forest Service [10-JV-11242303-014] FX This work was supported under Joint Venture Agreement 10-JV-11242303-014 from the USDA Forest Service. NR 33 TC 0 Z9 0 U1 11 U2 11 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0960-2585 EI 1475-2735 J9 SEED SCI RES JI Seed Sci. Res. PD JUN PY 2016 VL 26 IS 2 BP 148 EP 152 DI 10.1017/S0960258516000052 PG 5 WC Plant Sciences SC Plant Sciences GA DP8AV UT WOS:000378721200006 ER PT J AU Flesch, EP Garrott, RA White, PJ Brimeyer, D Courtemanch, AB Cunningham, JA Dewey, SR Fralick, GL Loveless, K McWhirter, DE Miyasaki, H Pils, A Sawaya, MA Stewart, ST AF Flesch, Elizabeth P. Garrott, Robert A. White, P. J. Brimeyer, Doug Courtemanch, Alyson B. Cunningham, Julie A. Dewey, Sarah R. Fralick, Gary L. Loveless, Karen McWhirter, Doug E. Miyasaki, Hollie Pils, Andrew Sawaya, Michael A. Stewart, Shawn T. TI Range Expansion and Population Growth of Nonnative Mountain Goats in the Greater Yellowstone Area: Challenges for Management SO WILDLIFE SOCIETY BULLETIN LA English DT Article DE alpine; bighorn sheep; invasive species; mountain goats; Oreamnos americanus; Ovis canadensis; population management; ungulate ID BIGHORN SHEEP; PNEUMONIA EPIZOOTICS; DYNAMICS AB Population growth and range expansion of nonnative species can potentially disrupt ecosystem function or add conservation value to an area, and evaluation of possible impacts can be a challenge for managers. Nonnative populations of mountain goats (Oreamnos americanus) are present in the Greater Yellowstone Area (GYA) in the U.S. states of Idaho, Montana, and Wyoming because of historical introduction events, but their population trend and range have not been assessed across the area. We used 6,701 location records from 1947 to 2015 to map mountain goat distribution and evaluate, in a descriptive manner, range overlap with native bighorn sheep (Ovis canadensis). We analyzed 136 survey counts using the Exponential Growth State-Space model to estimate population trends and abundance. Mountain goats dispersed 50-85km from introduction sites to occupy all mountain ranges in the northern GYA and 30-40 km to occupy new areas in the southern GYA. Mountain goat numbers increased in nearly all count units, with the strongest growth rates estimated in areas more recently colonized. Using moderate detection probability (0.70), we estimated approximately 2,355 mountain goats in the GYA. Although not tested in our analysis, the gradual range expansion and population growth rates were consistent with density-dependent processes observed in other introduced large herbivores and demonstrate that mountain goats can successfully disperse over unsuitable locales to colonize new areas. Therefore, we expect mountain goat populations will continue to expand into unoccupied mountain ranges that contain significant numbers of bighorn sheep unless specific management actions are implemented to address their population growth. (C) 2016 The Wildlife Society. C1 [Flesch, Elizabeth P.; Garrott, Robert A.; Sawaya, Michael A.] Montana State Univ, Dept Ecol, Fish & Wildlife Ecol & Management Program, 310 Lewis Hall, Bozeman, MT 59717 USA. [White, P. J.] Natl Pk Serv, POB 168, Yellowstone Natl Pk, WY 82190 USA. [Brimeyer, Doug; Courtemanch, Alyson B.] Wyoming Game & Fish Dept, 420 N Cache,POB 67, Jackson, WY 83001 USA. [Cunningham, Julie A.] Montana Fish Wildlife & Pk, 1400 S 19th Ave, Bozeman, MT 59717 USA. [Dewey, Sarah R.] Natl Pk Serv, Grand Teton Natl Pk,POB 170, Moose, WY 83012 USA. [Fralick, Gary L.] Wyoming Game & Fish Dept, POB 1022, Thayne, WY 83127 USA. [Loveless, Karen] Montana Fish Wildlife & Pk, 1354 Highway 10 W, Livingston, MT 59047 USA. [McWhirter, Doug E.] Wyoming Game & Fish Dept, 2820 State Highway 120, Cody, WY 82414 USA. [Miyasaki, Hollie] Idaho Dept Fish & Game, 4279 Commerce Circle, Idaho Falls, ID 83401 USA. [Pils, Andrew] US Forest Serv, Shoshone Natl Forest, 203A Yellowstone Ave, Cody, WY 82414 USA. [Stewart, Shawn T.] Montana Fish Wildlife & Pk, POB 581, Red Lodge, MT 59068 USA. RP Flesch, EP (reprint author), Montana State Univ, Dept Ecol, Fish & Wildlife Ecol & Management Program, 310 Lewis Hall, Bozeman, MT 59717 USA. EM elizabeth.flesch@yahoo.com FU National Park Service; Canon USA Inc. through the Yellowstone Park Foundation; U.S. Forest Service; Montana and Wyoming chapters of The Wild Sheep Foundation; Montana State University; Idaho Department of Fish and Game; Montana Department of Fish, Wildlife and Parks; Wyoming Game and Fish Department; Rocky Mountains Cooperative Ecosystem Studies Unit; Office of the Provost; Office of the Vice President for Research FX Primary funding for the data synthesis was provided by the National Park Service and Canon USA Inc. through the Yellowstone Park Foundation, with additional funding from the U.S. Forest Service, and the Montana and Wyoming chapters of The Wild Sheep Foundation. K. Tonnessen and the Rocky Mountains Cooperative Ecosystem Studies Unit facilitated cooperative funding agreements. The senior author was supported by Montana State University's Undergraduate Scholars Program, the Office of the Provost, and the Office of the Vice President for Research while performing this research as an undergraduate student. Funding for the population surveys was provided by the Idaho Department of Fish and Game; Montana Department of Fish, Wildlife and Parks; Wyoming Game and Fish Department; and the National Park Service. U.S. Forest Service biologist J. Canfield and the Wild Sheep Foundation conservation director, K. Hurley, provided administrative support. Montana Fish, Wildlife and Park biologists T. Lemke and J. Paugh contributed data. C. Butler, J. DeVoe, and M. O'Reilly provided technical support. B. Lowrey and C. Butler provided helpful comments on the manuscript. We thank the Associate Editor and 2 anonymous reviewers for their suggestions that improved a previous version of this manuscript. NR 57 TC 1 Z9 1 U1 13 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1938-5463 J9 WILDLIFE SOC B JI Wildl. Soc. Bull. PD JUN PY 2016 VL 40 IS 2 BP 241 EP 250 DI 10.1002/wsb.636 PG 10 WC Biodiversity Conservation SC Biodiversity & Conservation GA DR0ML UT WOS:000379601500006 ER PT J AU Masters, RE Montague, WG Cram, DS AF Masters, Ronald E. Montague, Warren G. Cram, Douglas S. TI Northern Bobwhite Autumn and Winter Food Habits in Restored Pine-Bluestem Habitats SO WILDLIFE SOCIETY BULLETIN LA English DT Article DE Arkansas; Colinus virginianus; food habits; foods; habitat quality; northern bobwhite; pine-bluestem; prescribed burning; restoration; wildlife stand improvement ID RED-COCKADED WOODPECKERS; GRASSLAND RESTORATION; ENERGY VALUES; QUAIL AB We determined autumn and winter food habits of hunter-harvested northern bobwhites (Colinus virginianus; n = 388) over 11 years (1998-2009) in a matrix of restored shortleaf pine-bluestem (Pinus echinata-Andropogon-Schizachyrium), and unthinned and unburned stands on the Ouachita National Forest, Arkansas, USA. Restoration treatments involved thinning of midstory hardwoods and midstory and co-dominant shortleaf pine in a process called wildlife stand improvement (WSI). The WSI was followed by February or March prescribed burns at 3-year intervals. Species richness of individual bobwhite crops ranged from 0 to 16 and differed among years but not months. Shortleaf pine seed, post oak (Quercus stellata) acorns, and various legume seed (Lespedeza spp., Desmodium spp.) occurred most often of 94 food categories/items (>= 110 species) found in quail crops. Shortleaf pine seed had among the highest gross energy (4.58 +/- 0.2 kcal/g), fat (21.8 +/- 0.6%), and crude protein (31.2 +/- 0.5%) of food items analyzed. Green vegetation occurred in 43.3% of all crops, the greatest of any food category, and also was high in crude protein (28.7%), but the lowest in gross energy (3.43 kcal/g). Invertebrates occurred in 35.6% of crops, but were high-ranked items in autumn and winter diets, particularly Orthopterans (12.4%), which were greatest in crude protein (64.3%). Average dry mass of crop contents was 1.38 g +/- 0.16 g. Dry mass of woody plant seeds comprised most of the diet, averaging 0.86 g +/- 0.17 g/crop. Bobwhites used a wide variety of plant and invertebrate food items that require or are benefited by open canopy conditions and frequent disturbance such as prescribed fire. (C) 2016 The Wildlife Society. C1 [Masters, Ronald E.] Tall Timbers Res Stn, Tallahassee, FL 32312 USA. [Masters, Ronald E.; Cram, Douglas S.] Oklahoma State Univ, Dept Forestry, Stillwater, OK 74078 USA. [Montague, Warren G.] US Forest Serv, Poteau Ranger Dist, Waldron, AR 72958 USA. [Masters, Ronald E.] Univ Wisconsin, Coll Nat Resources, Stevens Point, WI 54481 USA. [Cram, Douglas S.] New Mexico State Univ, Extens Anim Sci & Nat Resources, Las Cruces, NM 88003 USA. RP Masters, RE (reprint author), Tall Timbers Res Stn, Tallahassee, FL 32312 USA.; Masters, RE (reprint author), Oklahoma State Univ, Dept Forestry, Stillwater, OK 74078 USA.; Masters, RE (reprint author), Univ Wisconsin, Coll Nat Resources, Stevens Point, WI 54481 USA. EM ron.masters@uwsp.edu FU U.S. Forest Service; Tall Timbers Research Station; Department of Forestry, Oklahoma State University FX Financial support was provided by the U.S. Forest Service, Tall Timbers Research Station and Department of Forestry, Oklahoma State University. We thank R. Masters, L. Baggett, K. Gainey, J. Howard, and R. Kuzmic for assistance with sorting and weighing seeds from crops; and K. Gainey and L. Baggett for editorial assistance. We appreciate assistance from K. Galley with identification of insects and parts thereof. We thank the hunters, who collected, bagged, and labeled crops for later analysis especially J. Davis and L. Hedrick. We also thank J. Cox, B. Palmer, Associate Editor Guttery, and anonymous reviewers for useful comments on an earlier draft of the manuscript. NR 69 TC 0 Z9 0 U1 18 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1938-5463 J9 WILDLIFE SOC B JI Wildl. Soc. Bull. PD JUN PY 2016 VL 40 IS 2 BP 300 EP 309 DI 10.1002/wsb.646 PG 10 WC Biodiversity Conservation SC Biodiversity & Conservation GA DR0ML UT WOS:000379601500013 ER PT J AU Piaggio, AJ Cariappa, CA Straughan, DJ Neubaum, MA Dwire, M Krausman, PR Ballard, WB Bergman, DL Breck, SW AF Piaggio, Antoinette J. Cariappa, Chip A. Straughan, Dyan J. Neubaum, Melissa A. Dwire, Margaret Krausman, Paul R. Ballard, Warren B. Bergman, David L. Breck, Stewart W. TI A Noninvasive Method to Detect Mexican Wolves and Estimate Abundance SO WILDLIFE SOCIETY BULLETIN LA English DT Article DE alleles; Arizona; Blue Range Wolf Recovery Area; Canis lupus baileyi; fecal noninvasive DNA; mark-recapture; Mexican wolf; microsatellites ID INCORPORATING GENOTYPING ERROR; ESTIMATING POPULATION-SIZE; GRAY WOLF; CANIS-LUPUS; RENDEZVOUS SITES; CAPTURE; SAMPLES; MARK; PCR; PROBABILITY AB Monitoring wolf abundance is important for recovery efforts of Mexican wolves (Canis lupus baileyi) in the Blue Range Wolf Recovery Area in Arizona and New Mexico, USA. Although radiotelemetry has been a reliable method, collaring and tracking wolves in an expanding population will be prohibitively expensive and alternative methods to estimate abundance will become necessary. We applied 10 canid microsatellite loci to 235 Mexican wolf samples, 48 coyote (C. latrans) samples, and 14 domestic dog (C. lupus familiaris) samples to identify alleles that provide reliable separation of these species. We then evaluated an approach for prescreening, noninvasively collected DNA obtained from fecal samples to identify Mexican wolves. We generated complete genotypes for only those samples identified as probable Mexican wolves. We used these genotypes to estimate mark-recapture population estimates of Mexican wolves and compared these to known numbers of wolves in the study area. We collected fecal samples during 3 sampling periods in 2007-2008 and used Huggins-type mark-recapture models to estimate Mexican wolf abundance. We were able to generate abundance estimates with 95% confidence for 2 of 3 sampling periods. We estimated abundance to be 10 (95% CI = 6-34) during one sampling period when the known abundance was 10 and we estimated abundance to be 9 (95% CI = 6-30) during the other sampling period when the known abundance was 10. The application of this noninvasive method to estimate Mexican wolf abundance provides an alternative monitoring tool that could be useful for long-term monitoring of this and other recovering populations. Published 2016. This article is a U.S. Government work and is in the public domain in the USA. C1 [Piaggio, Antoinette J.; Neubaum, Melissa A.; Breck, Stewart W.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Cariappa, Chip A.; Ballard, Warren B.] Texas Tech Univ, Dept Nat Resources Management, Lubbock, TX 79409 USA. [Straughan, Dyan J.] US Fish & Wildlife Serv, Natl Forens Lab, Ashland, OR 97520 USA. [Dwire, Margaret] US Fish & Wildlife Serv, Albuquerque, NM 87113 USA. [Krausman, Paul R.] Univ Montana, Boone & Crockett Program Wildlife Conservat, Missoula, MT 59812 USA. [Bergman, David L.] US Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Phoenix, AZ 85021 USA. [Piaggio, Antoinette J.] 4101 LaPorte Ave, Ft Collins, CO 80521 USA. RP Piaggio, AJ (reprint author), Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA.; Piaggio, AJ (reprint author), 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM toni.j.piaggio@aphis.usda.gov OI Bergman, David/0000-0002-6757-643X FU Arizona Game and Fish Department; New Mexico Department of Game and Fish; U.S. Department of Agriculture/Animal and Plant Health Inspection Service/Wildlife Services; California Wolf Center; Dr. Donald and Sammie Bricker Foundation; Texas Tech University FX We are grateful to J. Oakleaf, S. Fain, and the late J. Morgart of the U.S. Fish and Wildlife Service for their cooperation, their ideas, and for the use of facilities and data made available to us. We are also grateful to C. Carillo of U.S. Department of Agriculture/Wildlife Services for his expert logistical support. He made sure that no task was delayed or went unfinished because we lacked a piece of equipment or personnel. We thank J. Fischer for assisting with Geographic Information System and developing Figure 1 of the study area. Private citizens (too numerous to mention individually) volunteered hundreds of hours of their free time to help with this project. We thank them for their contributions. This project was funded by the Arizona Game and Fish Department, New Mexico Department of Game and Fish, U.S. Department of Agriculture/Animal and Plant Health Inspection Service/Wildlife Services, California Wolf Center, Dr. Donald and Sammie Bricker Foundation, and Texas Tech University. The findings and conclusions in this article are those of the author(s) and do not necessarily represent the views of the U.S. Fish and Wildlife Service. We thank the anonymous reviewers and associate editors that contributed to refining this article. NR 60 TC 0 Z9 0 U1 19 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1938-5463 J9 WILDLIFE SOC B JI Wildl. Soc. Bull. PD JUN PY 2016 VL 40 IS 2 BP 321 EP 330 DI 10.1002/wsb.659 PG 10 WC Biodiversity Conservation SC Biodiversity & Conservation GA DR0ML UT WOS:000379601500016 ER PT J AU Fischer, JW Blass, CR Walter, WD Anderson, CW Lavelle, MJ Hall, WH VerCauteren, KC AF Fischer, Justin W. Blass, Chad R. Walter, W. David Anderson, Charles W. Lavelle, Michael J. Hall, Wayne H. VerCauteren, Kurt C. TI Evaluating a Strategy to Deliver Vaccine to White-tailed Deer at a Landscape Level SO WILDLIFE SOCIETY BULLETIN LA English DT Editorial Material DE bait; bovine tuberculosis; bTB; disease transmission; motion-activated cameras; nontarget species; Odocoileus virginianus; vaccine delivery; white-tailed deer ID MYCOBACTERIUM-BOVIS; ODOCOILEUS-VIRGINIANUS; FERAL SWINE; BAIT SITES; WISCONSIN; MOVEMENTS; PHARMACEUTICALS; MICHIGAN; RACCOONS AB Effective delivery of vaccines and other pharmaceuticals to wildlife populations is needed when zoonotic diseases pose a risk to public health and natural resources or have considerable economic consequences. The objective of our study was to develop a bait-distribution strategy for potential delivery of oral bovine tuberculosis (bTB) vaccine to white-tailed deer (Odocoileus virginianus) where deer are reservoirs for the disease. During 17 February and 2 March 2011, we created a grid of experimental bait stations (n = 64) on Sandhill Wildlife Management Area, Wisconsin, USA, to assess station densities needed to attract and deliver placebo baits to free-ranging white-tailed deer and look for associations among deer density, number of bait stations per deer, and bait consumption. We placed 1 L of commercially available alfalfa cubes at bait stations 652 m apart, and monitored stations with motion-activated cameras for 5 days to document visitation and consumption by deer and nontarget species. Deer discovered 38% of all bait stations within 37 hr, on average (SE = 3.91 hr), and consumed variable amounts of bait at each station. Deer were documented in 94% of all photographs of wildlife at bait stations. We found no correlation between bait consumption and deer density or the number of bait stations per deer. We provide the first information on use of baits by free-ranging deer and nontarget wildlife to eventually vaccinate deer against bTB at a landscape level. The results of this study can further the development of strategies in delivery of pharmaceuticals to free-ranging white-tailed deer. Published 2016. This article is a U.S. Government work and is in the public domain in the USA. C1 [Fischer, Justin W.; Blass, Chad R.; Anderson, Charles W.; Lavelle, Michael J.; VerCauteren, Kurt C.] Wildlife Serv, USDA, Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. [Walter, W. David] Penn State Univ, US Geol Survey, Penn Cooperat Fish & Wildlife Res Unit, 403 Forest Resources Bldg, University Pk, PA 16802 USA. [Hall, Wayne H.] Wisconsin Dept Nat Resources, Wisconsin Rapids, WI 54494 USA. [Anderson, Charles W.] Missouri Dept Conservat, 2901 W Truman Blvd, Jefferson City, MO 65109 USA. RP VerCauteren, KC (reprint author), Wildlife Serv, USDA, Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM kurt.c.vercauteren@aphis.usda.gov NR 26 TC 0 Z9 0 U1 7 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1938-5463 J9 WILDLIFE SOC B JI Wildl. Soc. Bull. PD JUN PY 2016 VL 40 IS 2 BP 394 EP 399 DI 10.1002/wsb.635 PG 6 WC Biodiversity Conservation SC Biodiversity & Conservation GA DR0ML UT WOS:000379601500027 ER PT J AU White, AM Manley, PN Tarbill, GL Richardson, TW Russell, RE Safford, HD Dobrowski, SZ AF White, A. M. Manley, P. N. Tarbill, G. L. Richardson, T. W. Russell, R. E. Safford, H. D. Dobrowski, S. Z. TI Avian community responses to post-fire forest structure: implications for fire management in mixed conifer forests SO ANIMAL CONSERVATION LA English DT Article DE avian community; forest fires; salvage logging; fire severity; post-fire management; hierarchical modeling; biodiversity; forest management ID CAVITY-NESTING BIRDS; WESTERN UNITED-STATES; PRESCRIBED FIRE; SPECIES RICHNESS; FUEL TREATMENTS; SIERRA-NEVADA; SEVERITY; WILDFIRE; CALIFORNIA; USA AB Fire is a natural process and the dominant disturbance shaping plant and animal communities in many coniferous forests of the western US. Given that fire size and severity are predicted to increase in the future, it has become increasingly important to understand how wildlife responds to fire and post-fire management. The Angora Fire burned 1243 hectares of mixed conifer forest in South Lake Tahoe, California. We conducted avian point counts for the first 3 years following the fire in burned and unburned areas to investigate which habitat characteristics are most important for re-establishing or maintaining the native avian community in post-fire landscapes. We used a multi-species occurrence model to estimate how avian species are influenced by the density of live and dead trees and shrub cover. While accounting for variations in the detectability of species, our approach estimated the occurrence probabilities of all species detected including those that were rare or observed infrequently. Although all species encountered in this study were detected in burned areas, species-specific modeling results predicted that some species were strongly associated with specific post-fire conditions, such as a high density of dead trees, open-canopy conditions or high levels of shrub cover that occur at particular burn severities or at a particular time following fire. These results indicate that prescribed fire or managed wildfire which burns at low to moderate severity without at least some high-severity effects is both unlikely to result in the species assemblages that are unique to post-fire areas or to provide habitat for burn specialists. Additionally, the probability of occurrence for many species was associated with high levels of standing dead trees indicating that intensive post-fire harvest of these structures could negatively impact habitat of a considerable proportion of the avian community. C1 [White, A. M.; Manley, P. N.; Tarbill, G. L.] US Forest Serv, Pacific Southwest Res Stn, USDA, Davis, CA 95618 USA. [Tarbill, G. L.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. [Richardson, T. W.] Tahoe Inst Nat Sci, Incline Village, NV USA. [Russell, R. E.] USGS, Natl Wildlife Hlth Ctr, Madison, WI USA. [Safford, H. D.] US Forest Serv, USDA, Pacific Southwest Reg, Vallejo, CA USA. [Safford, H. D.] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. [Dobrowski, S. Z.] Univ Montana, Coll Forestry & Conservat, Missoula, MT 59812 USA. RP White, AM (reprint author), US Forest Serv, Pacific Southwest Res Stn, USDA, Davis, CA 95618 USA. EM angelawhite@fs.fed.us FU California Tahoe Conservancy; Southern Nevada Public Lands Management Act research grant program FX Post-fire field studies are a dirty business. Field data collection was conducted by a large number of hardworking individuals; special thanks are owed to C. Carlson, D. Gaube and B. Campos for leading the field data collection. This project was made possible through two grants provided by the California Tahoe Conservancy and the Southern Nevada Public Lands Management Act research grant program. We would like to thank the reviewers who provided invaluable comments that improved this paper. The use of trade, product or firm names is for descriptive purposes only and does not imply endorsement by the US government. NR 48 TC 1 Z9 1 U1 18 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1367-9430 EI 1469-1795 J9 ANIM CONSERV JI Anim. Conserv. PD JUN PY 2016 VL 19 IS 3 BP 256 EP 264 DI 10.1111/acv.12237 PG 9 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DQ1CU UT WOS:000378939500008 ER PT J AU Brand, L Beltran, A Hughes, S O'Connor, T Baranowski, J Nicklas, T Chen, TA Dadabhoy, HR Diep, CS Buday, R Baranowski, T AF Brand, Leah Beltran, Alicia Hughes, Sheryl O'Connor, Teresia Baranowski, Janice Nicklas, Theresa Chen, Tzu-An Dadabhoy, Hafza R. Diep, Cassandra S. Buday, Richard Baranowski, Tom TI Assessing Feedback in a Mobile Videogame SO GAMES FOR HEALTH JOURNAL LA English DT Article ID VEGETABLE PARENTING PRACTICES; PHYSICAL-ACTIVITY; BEHAVIOR-CHANGE; ALPHA TEST; METAANALYSIS AB Background: Player feedback is an important part of serious games, although there is no consensus regarding its delivery or optimal content. Mommio is a serious game designed to help mothers motivate their preschoolers to eat vegetables. The purpose of this study was to assess optimal format and content of player feedback for use in Mommio. Materials and Methods: The current study posed 36 potential Mommio gameplay feedback statements to 20 mothers using a Web survey and interview. Mothers were asked about the meaning and helpfulness of each feedback statement. Results: Several themes emerged upon thematic analysis, including identifying an effective alternative in the case of corrective feedback, avoiding vague wording, using succinct and correct grammar, avoiding provocation of guilt, and clearly identifying why players' game choice was correct or incorrect. Conclusions: Guidelines are proposed for future feedback statements. C1 [Brand, Leah; Beltran, Alicia; Hughes, Sheryl; O'Connor, Teresia; Baranowski, Janice; Nicklas, Theresa; Chen, Tzu-An; Dadabhoy, Hafza R.; Diep, Cassandra S.; Baranowski, Tom] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Buday, Richard] Archimage, Houston, TX USA. RP Baranowski, T (reprint author), Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM tbaranow@bcm.edu NR 14 TC 1 Z9 1 U1 3 U2 3 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2161-783X EI 2161-7856 J9 GAMES HEALTH J JI Games Health J. PD JUN PY 2016 VL 5 IS 3 BP 203 EP 208 DI 10.1089/g4h.2015.0056 PG 6 WC Health Policy & Services; Public, Environmental & Occupational Health; Rehabilitation SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Rehabilitation GA DQ4EH UT WOS:000379156200007 PM 27058403 ER PT J AU Bamberg, JB Martin, MW Abad, J Jenderek, MM Tanner, J Donnelly, DJ Nassar, AMK Veilleux, RE Novy, RG AF Bamberg, J. B. Martin, M. W. Abad, J. Jenderek, M. M. Tanner, J. Donnelly, D. J. Nassar, A. M. K. Veilleux, R. E. Novy, R. G. TI In vitro technology at the US Potato Genebank SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT LA English DT Review DE Potato; Solanum; Genebank; Germplasm collection; In vitro conservation ID SOLANUM-TUBEROSUM-L; BROAD-SPECTRUM RESISTANCE; SHOOT-TIP CULTURES; ANTHER CULTURE; LATE BLIGHT; SOMATIC HYBRIDS; DIPLOID POTATO; LEAFROLL-VIRUS; PHYTOPHTHORA-INFESTANS; MELOIDOGYNE-CHITWOODI AB The US Potato Genebank in Sturgeon Bay, Wisconsin, is the national germplasm collection for the world's most important vegetable crop. It contains about 6,000 accessions of 100 species of tuber-bearing relatives of Solanum tuberosum. The potato of commerce is a clonal crop susceptible to many systemic pathogens, so the genebank routinely uses in vitro clonal maintenance and distribution for named cultivars. In vitro management is also the tool of choice for various breeding and genetic stocks of interest to breeders and researchers. Long-term backup cryopreservation of clones is done at the base collection in Fort Collins, Colorado. In vitro techniques also play an important role in virus elimination from clones. Recently, in vitro propagation has expanded to the temporary safekeeping of meristem propagules in antimicrobial medium during plant collecting expeditions in the southwest USA. The genebank's mission includes promoting technology that supports expanded use of the germplasm, in particular, finding ways to overcome interspecific hybridization barriers. Thus, in vitro techniques such as pollen viability testing, ploidy manipulation, protoplast fusion, and embryo rescue have contributed technology for major advances in interspecific hybridization, utilization of noncommercial species, and introgression of genes from wild near-relatives. Finally, advancing in vitro technology holds promise as a tool for mass bioassay and selection of seeds, pollen, or somaclones for useful traits. C1 [Bamberg, J. B.; Martin, M. W.] US Potato Genebank, 4312 Hwy 42, Sturgeon Bay, WI 54235 USA. [Abad, J.] USDA APHIS PPQ FO Plant Germplasm Quarantine Prog, Beltsville, MD 20705 USA. [Jenderek, M. M.; Tanner, J.] USDA ARS, Natl Ctr Genet Resources Preservat, 1111 Mason St, Ft Collins, CO 80521 USA. [Donnelly, D. J.] McGill Univ, Dept Plant Sci, 21,111 Lakeshore Rd, Ste Anne De Bellevue, PQ H9X 3V9, Canada. [Nassar, A. M. K.] Damanhour Univ, Dept Plant Protect, Fac Agr, Damanhour 22516, Albeheira, Egypt. [Veilleux, R. E.] Virginia Tech, Dept Hort, Blacksburg, VA 24061 USA. [Novy, R. G.] USDA ARS, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. RP Bamberg, JB (reprint author), US Potato Genebank, 4312 Hwy 42, Sturgeon Bay, WI 54235 USA. EM John.Bamberg@ars.usda.gov OI Nassar, Atef/0000-0002-0394-1530 NR 119 TC 0 Z9 0 U1 21 U2 26 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1054-5476 EI 1475-2689 J9 IN VITRO CELL DEV-PL JI In Vitro Cell. Dev. Biol.-Plant PD JUN PY 2016 VL 52 IS 3 BP 213 EP 225 DI 10.1007/s11627-016-9753-x PG 13 WC Plant Sciences; Cell Biology; Developmental Biology SC Plant Sciences; Cell Biology; Developmental Biology GA DQ5TD UT WOS:000379267000001 ER PT J AU Poothong, S Reed, BM AF Poothong, Sukalya Reed, Barbara M. TI Optimizing shoot culture media for Rubus germplasm: the effects of NH4 (+), NO3 (-), and total nitrogen SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT LA English DT Article DE Medium optimization; Mixture component design; Nitrogen source; Potassium ID TOBACCO TISSUE CULTURES; SOMATIC EMBRYOGENESIS; INORGANIC NITROGEN; MINERAL-NUTRITION; GROWTH; POTASSIUM; SEEDLINGS; L.; REQUIREMENTS; FORM AB A mixture component design was applied to testing the Murashige and Skoog (MS) medium nitrogen salts (NH4NO3 and KNO3) in order to optimize growth medium nitrogen for shoot cultures of red raspberries and a range of Rubus germplasm. All other components were those of MS medium with 2.5x MS mesos (CaCl2, MgSO4, KH2PO4) nutrients developed in an earlier study. Eight red raspberry (Rubus idaeus L.) cultivars and one accession of Rubus odoratus L. were evaluated for overall quality, shoot length, proliferation, leaf characteristics, and physiological disorders after 9-wk growth on each treatment. Twelve Rubus genotypes were later tested with the five best treatments. The concentration of NO3 (-) in the medium was the most significant factor for improved shoot quality for most cultivars. The effects of the two-component mixture (NH4 (+) and K+) and interactions between the mixture components and NO3 (-) were found in many growth responses. Combinations of intermediate or high NO3 (-) (40-60 mM), and intermediate to high NH4 (+) (30-45 mM) produced the best growth in most cultivars. The 2.5x mesos MS medium with standard MS nitrogen (20 mM NH4 (+) and 40 mM NO3 (-)) produced significantly better growth for most genotypes than unmodified MS. Three other nitrogen treatments also improved shoot quality for many genotypes: lower NH4 (+) with lower total nitrogen (10 mM NH4 (+) and 40 mM NO3 (-)) or intermediate or high concentrations of both nitrogen components and higher total nitrogen (30 mM NH4 (+) and 60 mM NO3 (-); 45 mM NH4 (+) and 60 mM NO3 (-)) than that of MS medium. C1 [Poothong, Sukalya] Univ Phayao, Sch Agr & Nat Resources, 19 Moo 2 Tambon Maeka, Muang Dist 56000, Phayao, Thailand. [Reed, Barbara M.] USDA ARS, Natl Clonal Germplasm Repository, 33447 Peoria Rd, Corvallis, OR 97333 USA. RP Reed, BM (reprint author), USDA ARS, Natl Clonal Germplasm Repository, 33447 Peoria Rd, Corvallis, OR 97333 USA. EM sukalya_p@hotmail.com; reedba@onid.oregonstate.edu FU US Department of Agriculture, Agricultural Research Service CRIS project [5358-21000-033D]; Ministry of Science and Technology (Thailand) FX Funding for this study was from the US Department of Agriculture, Agricultural Research Service CRIS project 5358-21000-033D. Sukalya Poothong acknowledges the scholarship from the Ministry of Science and Technology (Thailand) for her Ph.D. studies. NR 21 TC 0 Z9 0 U1 4 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1054-5476 EI 1475-2689 J9 IN VITRO CELL DEV-PL JI In Vitro Cell. Dev. Biol.-Plant PD JUN PY 2016 VL 52 IS 3 BP 265 EP 275 DI 10.1007/s11627-016-9750-0 PG 11 WC Plant Sciences; Cell Biology; Developmental Biology SC Plant Sciences; Cell Biology; Developmental Biology GA DQ5TD UT WOS:000379267000006 ER PT J AU Elwell, SL Griswold, T Elle, E AF Elwell, Sherri L. Griswold, Terry Elle, Elizabeth TI Habitat type plays a greater role than livestock grazing in structuring shrubsteppe plant-pollinator communities SO JOURNAL OF INSECT CONSERVATION LA English DT Article DE Biodiversity; Community composition; Insect conservation; Livestock grazing; Pollinators; Shrubsteppe ID WESTERN NORTH-AMERICA; FLOWERING PLANTS; BOMBUS SPP.; GRASSLAND; DIVERSITY; BEES; CONSERVATION; MANAGEMENT; LANDSCAPE; INTENSITY AB Livestock grazing is a widespread grassland disturbance and can negatively impact biodiversity. Pollinators constitute a vital component of grassland ecosystems, but the impact of grazing on pollinator diversity has seldom been evaluated in North America. We assessed vegetation structure, and pollinator and flowering plant abundance, richness, diversity, and community composition in four pairs of spring-grazed/ungrazed sites in south-central British Columbia, Canada. We also investigated whether pollinator or floral communities differed between the two threatened shrubsteppe habitat types we sampled-antelope-brush and big sagebrush shrubsteppe. Pan-trapping surveys captured 5907 bees, flies, beetles, wasps and butterflies constituting 253 species. We found that the percent cover of shrubs and bare soil increased with grazing, while the height of grasses and forbs decreased. In contrast, pollinator and flowering plant abundance, richness, diversity, and community composition were not significantly affected by grazing. Flowering plant and pollinator community composition did differ significantly between shrubsteppe habitats. Our results indicate that grasslands in North America, when managed responsibly, can maintain pollinator and flowering plant diversity under grazing pressure. The continued effort of land managers to balance ecological integrity and economic viability will be important for the conservation of grassland pollination systems. C1 [Elwell, Sherri L.; Elle, Elizabeth] Simon Fraser Univ, Evolutionary & Behav Ecol Res Grp, Dept Biol Sci, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada. [Griswold, Terry] Utah State Univ, USDA ARS Bee Biol & Systemat Lab, 5310 Old Main Hill, Logan, UT 84322 USA. [Griswold, Terry] Utah State Univ, Dept Biol, 5310 Old Main Hill, Logan, UT 84322 USA. RP Elle, E (reprint author), Simon Fraser Univ, Evolutionary & Behav Ecol Res Grp, Dept Biol Sci, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada. EM eelle@sfu.ca FU Natural Sciences and Engineering Research Council of Canada's "Canadian Pollination Initiative" (NSERC-CANPOLIN); NSERC CGS-M award FX We are grateful to J. Pendray and T. Holland for field assistance, G. Gielens and J. Pendray for lab assistance, and L. Neame, J. Gibbs, J. Hammond, and C. Sheffield for assistance with insect identifications. Site access was provided by Wade Clifton, The Nature Trust of BC, Canadian Wildlife Service, BC Provincial Parks, and BC Ministry of Forest and Range. D. Green, J. Moore, S. Chamberlain, C. Mayer, and anonymous reviewers provided comments on earlier drafts of the manuscript. Financial support was provided by the Natural Sciences and Engineering Research Council of Canada's "Canadian Pollination Initiative" (NSERC-CANPOLIN) and an NSERC CGS-M award to S.L. Elwell. This is publication 62 of NSERC-CANPOLIN. NR 61 TC 0 Z9 0 U1 23 U2 32 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1366-638X EI 1572-9753 J9 J INSECT CONSERV JI J. Insect Conserv. PD JUN PY 2016 VL 20 IS 3 BP 515 EP 525 DI 10.1007/s10841-016-9884-8 PG 11 WC Biodiversity Conservation; Entomology SC Biodiversity & Conservation; Entomology GA DQ2LU UT WOS:000379034600016 ER PT J AU Shi, LL Guo, YF Dong, CL Huddleston, J Yang, H Han, XL Fu, AS Li, Q Li, N Gong, SY Lintner, KE Ding, Q Wang, Z Hu, J Wang, DP Wang, F Wang, L Lyon, GJ Guan, YT Shen, YF Evgrafov, OV Knowles, JA Thibaud-Nissen, F Schneider, V Yu, CY Zhou, LB Eichler, EE So, KF Wang, K AF Shi, Lingling Guo, Yunfei Dong, Chengliang Huddleston, John Yang, Hui Han, Xiaolu Fu, Aisi Li, Quan Li, Na Gong, Siyi Lintner, Katherine E. Ding, Qiong Wang, Zou Hu, Jiang Wang, Depeng Wang, Feng Wang, Lin Lyon, Gholson J. Guan, Yongtao Shen, Yufeng Evgrafov, Oleg V. Knowles, James A. Thibaud-Nissen, Francoise Schneider, Valerie Yu, Chack-Yung Zhou, Libing Eichler, Evan E. So, Kwok-Fai Wang, Kai TI Long-read sequencing and de novo assembly of a Chinese genome SO NATURE COMMUNICATIONS LA English DT Article ID GENETIC-VARIATION; STRUCTURAL VARIATION; ALIGNMENT; HAPLOTYPE; FRAMEWORK; RNA; MAP AB Short-read sequencing has enabled the de novo assembly of several individual human genomes, but with inherent limitations in characterizing repeat elements. Here we sequence a Chinese individual HX1 by single-molecule real-time (SMRT) long-read sequencing, construct a physical map by NanoChannel arrays and generate a de novo assembly of 2.93 Gb (contig N50: 8.3 Mb, scaffold N50: 22.0 Mb, including 39.3Mb N-bases), together with 206Mb of alternative haplotypes. The assembly fully or partially fills 274 (28.4%) N-gaps in the reference genome GRCh38. Comparison to GRCh38 reveals 12.8Mb of HX1-specific sequences, including 4.1Mb that are not present in previously reported Asian genomes. Furthermore, long-read sequencing of the transcriptome reveals novel spliced genes that are not annotated in GENCODE and are missed by short-read RNA-Seq. Our results imply that improved characterization of genome functional variation may require the use of a range of genomic technologies on diverse human populations. C1 [Shi, Lingling; Li, Na; Gong, Siyi; Zhou, Libing; So, Kwok-Fai] Jinan Univ, Guangdong Hongkong Macau Inst CNS Regenerat, Guangzhou 510632, Guangdong, Peoples R China. [Shi, Lingling; Zhou, Libing; So, Kwok-Fai] Jinan Univ, Minist Educ, Joint Int Res Lab CNS Regenerat, Guangzhou 510632, Guangdong, Peoples R China. [Shi, Lingling; Zhou, Libing; So, Kwok-Fai] Nantong Univ, Coinnovat Ctr Neuroregenerat, Nantong 226001, Peoples R China. [Guo, Yunfei; Dong, Chengliang; Yang, Hui; Li, Quan; Evgrafov, Oleg V.; Knowles, James A.; Wang, Kai] Univ Southern Calif, Zilkha Neurogenet Inst, Los Angeles, CA 90089 USA. [Huddleston, John; Eichler, Evan E.] Univ Washington, Howard Hughes Med Inst, Dept Genome Sci, Seattle, WA 98195 USA. [Han, Xiaolu] Univ Southern Calif, Keck Sch Med, Genet Mol & Cellular Biol Program, Los Angeles, CA 90089 USA. [Fu, Aisi; Ding, Qiong; Wang, Zou] Wuhan Inst Biotechnol, Wuhan 430000, Peoples R China. [Lintner, Katherine E.; Yu, Chack-Yung] Ohio State Univ, Dept Pediat, Columbus, OH 43205 USA. [Lintner, Katherine E.; Yu, Chack-Yung] Nationwide Childrens Hosp, Res Inst, Columbus, OH 43205 USA. [Hu, Jiang; Wang, Depeng] Next Biosci, Wuhan 430000, Peoples R China. [Wang, Feng] Wuhan Inst Technol, Sch Chem Engn & Pharm, Wuhan 430000, Peoples R China. [Wang, Lin] Huazhong Univ Sci & Technol, Union Hosp, Ctr Tissue Engn & Regenerat Med, Wuhan 430022, Peoples R China. [Lyon, Gholson J.] Cold Spring Harbor Lab, Stanley Inst Cognit Genom, New York, NY 11797 USA. [Guan, Yongtao] USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Dept Mol & Human Genet,Baylor Coll Med, Houston, TX 77030 USA. [Shen, Yufeng] Columbia Univ, Dept Syst Biol, New York, NY 10032 USA. [Shen, Yufeng] Columbia Univ, Dept Biomed Informat, New York, NY 10032 USA. [Evgrafov, Oleg V.; Knowles, James A.; Wang, Kai] Univ Southern Calif, Keck Sch Med, Dept Psychiat & Behav Sci, Los Angeles, CA 90033 USA. [Thibaud-Nissen, Francoise; Schneider, Valerie] US Natl Lib Med, Natl Ctr Biotechnol Informat, Bethesda, MD 20894 USA. [So, Kwok-Fai] Univ Hong Kong, Dept Ophthalmol, Hong Kong, Hong Kong, Peoples R China. [So, Kwok-Fai] Univ Hong Kong, State Key Lab Brain & Cognit Sci, Hong Kong, Hong Kong, Peoples R China. RP Zhou, LB; So, KF (reprint author), Jinan Univ, Guangdong Hongkong Macau Inst CNS Regenerat, Guangzhou 510632, Guangdong, Peoples R China.; Zhou, LB; So, KF (reprint author), Jinan Univ, Minist Educ, Joint Int Res Lab CNS Regenerat, Guangzhou 510632, Guangdong, Peoples R China.; Zhou, LB; So, KF (reprint author), Nantong Univ, Coinnovat Ctr Neuroregenerat, Nantong 226001, Peoples R China.; Wang, K (reprint author), Univ Southern Calif, Zilkha Neurogenet Inst, Los Angeles, CA 90089 USA.; Wang, K (reprint author), Univ Southern Calif, Keck Sch Med, Dept Psychiat & Behav Sci, Los Angeles, CA 90033 USA.; So, KF (reprint author), Univ Hong Kong, Dept Ophthalmol, Hong Kong, Hong Kong, Peoples R China.; So, KF (reprint author), Univ Hong Kong, State Key Lab Brain & Cognit Sci, Hong Kong, Hong Kong, Peoples R China. EM libingzh@gmail.com; hrmaskf@hku.hk; kaiwang@usc.edu FU NIH [HG006465, MH108728, HG007635, HG002385]; National Natural Science Foundation of China [31400922]; Program of Introducing Talents of Discipline to Universities [B14036]; Leading Talents of Guangdong FX We thank the staff members of the Wuhan Institute of Biotechnology for generating the long-read DNA sequencing data, and thank Pacific Biosciences for generating the long-read RNA sequencing data. We thank Ahmed Naguib from BioNano Genomics for providing technical assistance. This project is supported by NIH grant HG006465 and MH108728 (K.W.), HG007635 and HG002385 (E.E.E.), the National Natural Science Foundation of China 31400922 (L.S.), the Program of Introducing Talents of Discipline to Universities B14036 (L.S. and S.K.F.) and Leading Talents of Guangdong 2013 (S.K.F.). E.E.E. is an investigator of the Howard Hughes Medical Institute. NR 41 TC 7 Z9 7 U1 11 U2 15 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD JUN PY 2016 VL 7 AR 12065 DI 10.1038/ncomms12065 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DQ3OO UT WOS:000379111800001 PM 27356984 ER PT J AU Singh, SK Reddy, VR AF Singh, Shardendu K. Reddy, Vangimalla R. TI Methods of mesophyll conductance estimation: its impact on key biochemical parameters and photosynthetic limitations in phosphorus-stressed soybean across CO2 SO PHYSIOLOGIA PLANTARUM LA English DT Article ID CARBON-ISOTOPE DISCRIMINATION; ELECTRON-TRANSPORT RATE; CHLOROPHYLL FLUORESCENCE MEASUREMENTS; DIOXIDE RESPONSE CURVES; COMBINED GAS-EXCHANGE; INTERNAL CONDUCTANCE; DIFFUSION CONDUCTANCE; CARBOXYLASE-OXYGENASE; STOMATAL CONDUCTANCE; SPECIFICITY FACTOR AB Despite the development of various methods, the rapid estimation of mesophyll conductance (g(m)) for a large number of samples is still a daunting challenge. Although the accurate estimation of g(m) is critical to partition photosynthetic limitations by stomatal (L-s) and mesophyll (L-m) conductance and by photo-biochemical (L-b) processes, the impact of various g(m) estimation methods on this is ambiguous. As phosphorus (P) starvation and elevated CO2 (eCO(2)) strongly affect photosynthetic processes, their combined effect on the proportional changes in these limitations are not well understood. To investigate this, while also evaluating distinct recent methods of g(m) estimation sharing few common theories and assumptions, soybean was grown under a range of P nutrition at ambient and eCO(2). Methods significantly affected g(m) and carboxylation efficiency (V-Cmax) but not other photosynthetic parameters. In all the methods, all photosynthetic parameters responded similarly to treatments. However, the percentage difference between VCmax assuming finite and infinite g(m) was highly inconsistent among methods. The primary mechanism responsible for P limitation to soybean photosynthesis was not CO2 diffusion limitations but L-b comprised of reduced chlorophyll, photochemistry and biochemical processes. The eCO(2) decreased L-b but increased L-m without affecting L-s across leaf P concentration. Although each method explored advances of our understanding about g(m) variability, they all require assumptions of varying degrees, which lead to the discrepancy in the g(m) values. Among the methods, the oxygen sensitivity-based g(m) estimation appeared to be suitable for the quick assessment of a large number of samples or genotypes. Digital tools are provided for the easy estimation of g(m) for some methods. C1 [Singh, Shardendu K.; Reddy, Vangimalla R.] ARS, Crop Syst & Global Change Lab, USDA, Beltsville, MD 20705 USA. [Singh, Shardendu K.] Univ Maryland, Wye Res & Educ Ctr, College Pk, MD 20742 USA. RP Singh, SK (reprint author), ARS, Crop Syst & Global Change Lab, USDA, Beltsville, MD 20705 USA.; Singh, SK (reprint author), Univ Maryland, Wye Res & Educ Ctr, College Pk, MD 20742 USA. EM shardendu.singh@ars.usda.gov NR 73 TC 2 Z9 3 U1 7 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0031-9317 EI 1399-3054 J9 PHYSIOL PLANTARUM JI Physiol. Plant. PD JUN PY 2016 VL 157 IS 2 BP 234 EP 254 DI 10.1111/ppl.12415 PG 21 WC Plant Sciences SC Plant Sciences GA DQ5RC UT WOS:000379261500009 PM 26806194 ER PT J AU Schwarting, HN Whitworth, RJ Chen, MS Cramer, G Maxwell, T AF Schwarting, Holly N. Whitworth, R. Jeff Chen, Ming-Shun Cramer, Gary Maxwell, Thomas TI Impact of Hessian fly, Mayetiola destructor, on Developmental Aspects of Hard Red Winter Wheat in Kansas SO SOUTHWESTERN ENTOMOLOGIST LA English DT Article ID GENE-MEDIATED RESISTANCE; DIPTERA-CECIDOMYIIDAE; SEEDLINGS; YIELD AB The Hessian fly, Mayetiola destructor (Say), has historically been a major pest of wheat, Triticum aestivum L., in Kansas. Resistant cultivars are used to protect seedling wheat plants from damage by Hessian fly larvae in the fall. However, it is not known if the cultivars are resistant after winter vernalization. Experiments in a greenhouse indicated that 'Armour', a cultivar considered intermediately resistant, remained resistant under low levels of infestation by Hessian fly, but kernel weight was significantly reduced by a mean of 0.13 g per spike when Hessian flies were abundant. In the field, Armour was not resistant post-vernalization, with infested plants having culm heights reduced by an average of 14.2 cm and kernel weight reduced by 0.10 g per spike compared to noninfested plants. In addition, infested plants had significantly fewer spikelets per spike, and fewer kernels per spike. 'Duster', a cultivar considered very resistant, was resistant to Hessian fly larvae in the greenhouse and the field, and even produced significantly heavier kernels when Hessian flies were abundant in the greenhouse. Infestation of susceptible cultivar 'Fuller' in the greenhouse and field resulted in culm heights reduced by up to a mean of 11.2 cm per spike as well as significantly fewer kernels per spikelet. Kernel weight was significantly reduced by a mean of over 0.1 g per spike in both the greenhouse and the field. The results suggested that all cultivars resistant to Hessian fly should be evaluated after vernalization to determine if each continued to be resistant into the spring. Little information is available on if and how feeding by Hessian fly larvae on more mature hard red winter wheat (Feekes 7 to 10) after vernalization impacts plants, aside from lodging. Growers are aware when Hessian fly larvae kill seedlings or cause plants lodging, but small and significant, although less dramatic, yield loss might occur undetected. C1 [Schwarting, Holly N.; Whitworth, R. Jeff; Chen, Ming-Shun] Kansas State Univ, Dept Entomol, 123 W Waters Hall, Manhattan, KS 66506 USA. [Chen, Ming-Shun] USDA ARS, Ctr Grain & Anim Hlth, Hard Winter Wheat Genet Res Unit, 1515 Coll Ave, Manhattan, KS 66506 USA. [Cramer, Gary] South Cent Expt Field, 10702 S Dean Rd, Hutchinson, KS 67501 USA. [Maxwell, Thomas] Cent Kansas Dist, 300 West Ash, Salina, KS 67402 USA. RP Schwarting, HN (reprint author), Kansas State Univ, Dept Entomol, 123 W Waters Hall, Manhattan, KS 66506 USA. FU Kansas Wheat Commission FX This research was partially funded by the Kansas Wheat Commission. Specimens from this research were deposited as voucher number 233 in the KSU Museum of Entomological and Prairie Arthropod Research. Contribution No. 16-260-J from the Kansas Agricultural Experiment Station. NR 21 TC 0 Z9 0 U1 2 U2 5 PU SOUTHWESTERN ENTOMOLOGICAL SOC PI DALLAS PA 17360 COIT RD, DALLAS, TX 75252-6599 USA SN 0147-1724 EI 2162-2647 J9 SOUTHWEST ENTOMOL JI Southw. Entomol. PD JUN PY 2016 VL 41 IS 2 BP 321 EP 329 PG 9 WC Entomology SC Entomology GA DQ5EM UT WOS:000379227800003 ER PT J AU Armstrong, JS Camelo, LA Zhu-Salzman, K Mitchell, FL AF Armstrong, J. S. Camelo, L. A. Zhu-Salzman, K. Mitchell, F. L. TI Effects of Cysteine Proteinase Inhibitors scN and E-64 on Southern Corn Rootworm(1) Larval Development SO SOUTHWESTERN ENTOMOLOGIST LA English DT Article ID PROTEASE INHIBITOR; COLEOPTERA; CHRYSOMELIDAE; SURVIVAL; PLANTS; ORYZACYSTATIN; LEPIDOPTERA; HOWARDI; WESTERN; MIDGUT AB The southern corn rootworm, Diabrotica undecimpunctata howardi Barber, can be a serious pest of peanut (Arachis hypogaea L.) pods. A laboratory bioassay was developed to test feeding cysteine proteinase inhibitors soyacystatin N (scN) and E-64 against southern corn rootworm reared on artificial diet to determine the effects on larval development and mortality. Soyacystatin N increased mortality of larvae and decreased larval development as measured by live weights at 4 and 7 d. Seven d weight gain for larvae in the control increased by 66%, while the 0.5, 1.0 and 2.0 mg/ml doses decreased by 50, 42, and 79%, respectively. The proteinase inhibitor proved to be a good candidate as a means of host-plant resistance for reducing larval development and increasing the chance to interrupt metabolism and increased mortality. E-64 also demonstrated significant larval mortality and decreased weight gain when fed through artificial diet. At 7 d, after feeding on the 0.50, 1.0, and 2.0 mg/ml doses, larval weights were decreased by 80, 150, and 190%, respectively, when compared to the controls. The deleterious effects of these inhibitors from these assays provides enough evidence that cultivars should be screened from the world collection to determine what lines may be available to use in the development of southern corn rootworm-resistant peanut lines. C1 [Armstrong, J. S.] USDA ARS, WPOFC Res Unit, 1301 North Western Rd, Stillwater, OK USA. [Camelo, L. A.] Monsanto Seeds, 243522 E Hatchet Rd, Oxnard, CA USA. [Zhu-Salzman, K.] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. [Mitchell, F. L.] Texas A&M AgriLife Res, 1229 N Highway 281, Stephenville, TX 76401 USA. RP Armstrong, JS (reprint author), USDA ARS, WPOFC Res Unit, 1301 North Western Rd, Stillwater, OK USA. EM Scott.armstrong@ars.usda.gov NR 26 TC 0 Z9 0 U1 4 U2 5 PU SOUTHWESTERN ENTOMOLOGICAL SOC PI DALLAS PA 17360 COIT RD, DALLAS, TX 75252-6599 USA SN 0147-1724 EI 2162-2647 J9 SOUTHWEST ENTOMOL JI Southw. Entomol. PD JUN PY 2016 VL 41 IS 2 BP 337 EP 345 PG 9 WC Entomology SC Entomology GA DQ5EM UT WOS:000379227800005 ER PT J AU Pritchard, JC Jacob, ME Ward, TJ Parsons, CT Kathariou, S Wood, MW AF Pritchard, Jessica C. Jacob, Megan E. Ward, Todd J. Parsons, Cameron T. Kathariou, Sophia Wood, Michael W. TI Listeria monocytogenes septicemia in an immunocompromised dog SO VETERINARY CLINICAL PATHOLOGY LA English DT Article DE Blood culture; canine; food contamination; infection; sepsis ID UNITED-STATES; FOODS AB An 11-year-old, male castrated, Boston Terrier was presented to the North Carolina State University College of Veterinary Medicine Small Animal Emergency Service with a 2-day history of progressive ataxia, left-sided head tilt, and anorexia. The dog had previously been diagnosed with chronic lymphoid leukemia and suspected immune-mediated destruction of his bone marrow precursor cells, possibly due to therapy with immunosuppressive dosages of prednisone and azathioprine. During the physical examination, abnormal findings included an increased body temperature and horizontal nystagmus. Diagnostic investigations included a computed tomography (CT) scan, which confirmed bilateral otitis media, and a blood culture, which was positive for Listeria monocytogenes serotype 4b (epidemic clone 1). Upon treatment with ampicillin/sulbactam, enrofloxacin, and minocycline, the dog became normothermic and the neurologic signs improved. L monocytogenes serotype 4b (epidemic clone 1) has been associated with outbreaks of human listeriosis originating from food contamination. Although rare case reports of Listeria spp. infection in dogs exist, an actual infection with the epidemic clone 1 strain has never before been reported in a dog. It should be included in the differential diagnoses in immunocompromised dogs with clinical signs of septicemia. C1 [Pritchard, Jessica C.] North Carolina State Univ, Coll Vet Med, Dept Clin Sci, Raleigh, NC USA. [Jacob, Megan E.] North Carolina State Univ, Dept Populat Hlth & Pathobiol, Raleigh, NC USA. [Ward, Todd J.] ARS, Microbial Genom & Bioproc Res Unit, USDA, Peoria, IL USA. [Parsons, Cameron T.; Kathariou, Sophia] North Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC USA. [Wood, Michael W.] Univ Wisconsin, Sch Vet Med, Dept Med Sci, 2015 Linden Dr, Madison, WI 53706 USA. RP Wood, MW (reprint author), Univ Wisconsin, Sch Vet Med, Dept Med Sci, 2015 Linden Dr, Madison, WI 53706 USA. EM mwood5@wisc.edu OI Parsons, Cameron/0000-0002-5307-2222 NR 23 TC 1 Z9 1 U1 0 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0275-6382 EI 1939-165X J9 VET CLIN PATH JI Vet. Clin. Pathol. PD JUN PY 2016 VL 45 IS 2 BP 254 EP 259 DI 10.1111/vcp.12363 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA DQ5EW UT WOS:000379228800007 PM 27218230 ER PT J AU Kuhl, JC Novy, RG Whitworth, JL Dibble, MS Schneider, B Hall, D AF Kuhl, Joseph C. Novy, Richard G. Whitworth, Jonathan L. Dibble, Margaret S. Schneider, Brian Hall, Darren TI Development of Molecular Markers Closely Linked to the Potato Leafroll Virus Resistance Gene, Rlr (etb) , for use in Marker-Assisted Selection SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Potato; Solanum tuberosum; S. etuberosum; Potato leafroll virus; Resistance ID TUBEROSUM SSP ANDIGENA; SOLANUM-TUBEROSUM; SOMATIC HYBRIDS; SERIES ETUBEROSA; PLRV RESISTANCE; MYZUS-PERSICAE; CHROMOSOME-XI; RECOMBINATION; GERMPLASM; PROGENY AB Potato leafroll virus (PLRV) is a major pathogen of potato with worldwide impact on seed and commercial production of potato. In North America, the primary potato cultivars grown by industry are not resistant to PLRV and require the application of insecticides to control the aphid vector of PLRV, so as to prevent PLRV infection of the crop. The Solanum etuberosum- derived dominant gene Rlr (etb) confers resistance to PLRV and has been mapped to chromosome 4. The previous closest marker to Rlr (etb) , C2_At1g42990, was mapped 13.6 cM from the gene in a BC3 population. Cleaved amplified polymorphic sequence markers were developed in the region surrounding C2_At1g42990 and mapped along with Rlr (etb) in 102 BC4 progeny. Sixteen markers were identified surrounding Rlr (etb) , with flanking markers at 2.1 and 9.3 cM. Two markers, 1367-8a and DMB32-11, both 2.1 cM from Rlr (etb) , are shown to be well suited for marker assisted selection. The development of additional molecular markers more closely linked to Rlr (etb) will greatly facilitate its use in potato breeding and its continued introgression into cultivated potato for the development of PLRV-resistant cultivars. C1 [Kuhl, Joseph C.; Dibble, Margaret S.] Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83844 USA. [Novy, Richard G.; Whitworth, Jonathan L.; Schneider, Brian; Hall, Darren] ARS, USDA, Small Grains & Potato Germplasm Res Unit, 1693 S 2700 W, Aberdeen, ID 83210 USA. RP Kuhl, JC (reprint author), Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83844 USA. EM jkuhl@uidaho.edu FU Agriculture and Food Research Initiative of the USDA National Institute of Food and Agriculture [2012-67013-19414]; University of Idaho, College of Agriculture and Life Sciences FX The authors would like to thank Dr. Craig Johnson, Brigham Young University-Idaho, Rexburg, Idaho for his statistical expertise and the advice and insights he provided on data transformation and subsequent statistical analyses. This project was supported by the Agriculture and Food Research Initiative competitive grant no. 2012-67013-19414 of the USDA National Institute of Food and Agriculture. Additional support was provided by the University of Idaho, College of Agriculture and Life Sciences. NR 41 TC 0 Z9 0 U1 2 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD JUN PY 2016 VL 93 IS 3 BP 203 EP 212 DI 10.1007/s12230-016-9496-6 PG 10 WC Agronomy SC Agriculture GA DP8ES UT WOS:000378731400001 ER PT J AU Booth, E Alyokhin, A AF Booth, Everett Alyokhin, Andrei TI Colorado Potato Beetle Response to Potato Plants Infected with Potato Virus Y and Potato Leafroll Virus SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Potato; Potato virus Y; Potato leafroll virus; Colorado potato beetle; Leptinotarsa decemlineata; Plant-pathogen-herbivore interactions ID MYZUS-PERSICAE HOMOPTERA; CHRYSOMELIDAE; COLEOPTERA; HERBIVORES; DEFENSE; PATHOGENS; APHIDIDAE; VECTOR; RESISTANCE; PHYSIOLOGY AB Agricultural systems are often simultaneously impacted by multiple stressors. In our study, we investigated the effects of two commonly occurring viruses of potato, Potato virus Y (PVY) and Potato leafroll virus (PLRV) on host plant selection and utilization across various spatial scales by the Colorado potato beetle. Adult beetles selected PLRV-infected foliage in the cage choice trails, and larvae that fed exclusively on PLRV-infected foliage developed into larger adults. PVY-infected foliage was avoided in the cage trials and had no effect on the weight of developing adults. In field trials, preference and utilization of potato plants was more dependent on plant size than on virus infection. These results demonstrate that while virus-infected plants can cause physiological changes that influence beetle preference, beetle abundance and/or distribution is driven by a more complex array of factors at the field level. Additionally, this study demonstrates the importance of investigating the applicability of laboratory findings at the field level. C1 [Booth, Everett; Alyokhin, Andrei] Univ Maine, Sch Biol & Ecol, 5722 Deering Hall, Orono, ME 04469 USA. [Booth, Everett] Anim & Plant Hlth Inspect Serv, USDA, Ctr Plant Hlth Sci & Technol, Buzzards Bay, MA 02542 USA. RP Alyokhin, A (reprint author), Univ Maine, Sch Biol & Ecol, 5722 Deering Hall, Orono, ME 04469 USA. EM andrei.alyokhin@umit.maine.edu FU [US-Hatch-ME021601] FX We thank Chandler Guerette, Shea Craig, Mason Raymond, Zane Willhide, Natalie Doody, Kyle Ryder, Cole Richards, and Kayla Richards for technical assistance, and Frank Drummond for providing advice on statistical analyses. This research was supported in part by US-Hatch-ME021601. This is Publication No. 3456 of the Maine Agricultural and Forest Experiment Station. NR 33 TC 0 Z9 0 U1 8 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD JUN PY 2016 VL 93 IS 3 BP 213 EP 223 DI 10.1007/s12230-016-9497-5 PG 11 WC Agronomy SC Agriculture GA DP8ES UT WOS:000378731400002 ER PT J AU Foley, ME AF Foley, Michael E. TI Germination of leafy spurge (Euphorbia esula L.) seeds under alternating temperatures: the effects of amplitude, midpoint, and imbibition in gibberellic acid SO CANADIAN JOURNAL OF PLANT SCIENCE LA English DT Article DE alternating temperature; dormant; germination; leafy spurge; seed ID TRANSCRIPTOME; RESPONSES; CONSTANT AB Germinability of dormant seeds or the capacity to germinate under particular conditions is sometimes enhanced by a diurnal alternating temperature regime relative to a constant temperature. Alternating temperature regimes vary in amplitude (difference between the minimum and maximum) and midpoint (average). The aim of this research was to test the effects of amplitude and midpoint regimes on germination of dormant leafy spurge seed from a Fargo, ND population in water and gibberellic acid (GA(3)). The optimal regimes for germination in both water and GA3 were 20:30 degrees C (16 h:8 h) and 20:35 degrees C (19 h:5 h), which provided amplitudes of 10 degrees C and 15 degrees C, respectively, with both conditions having a midpoint temperature of 23.3 degrees C. However, midpoint temperature of 22 degrees C to 27 degrees C were equally effective for germination under the 20:30 degrees C and 20:35 degrees C alternating temperature regimes. Additional alternating temperature regimes with amplitudes of 15 degrees C (15:30 degrees C and 10:25 degrees C), 20 degrees C (10:30 degrees C), and 25 degrees C (10:35 degrees C) generally displayed reduced germination in both water and GA(3), which is likely attributable to the low initial alternating temperature (<= 15 degrees C) component of the regime relative to the higher initial temperature of 20 degrees C for the optimum regimes of 20:30 degrees C and 20:35 degrees C. Under the alternating temperature regimes tested, seeds germinated in GA3 generally displayed higher germination (>= 80%) compared with seeds in water (30%-50%). C1 [Foley, Michael E.] USDA ARS, 1605 Albrecht Blvd, Fargo, ND 58102 USA. RP Foley, ME (reprint author), USDA ARS, 1605 Albrecht Blvd, Fargo, ND 58102 USA. EM michael.foley@ars.usda.gov NR 16 TC 0 Z9 0 U1 11 U2 11 PU AGRICULTURAL INST CANADA PI OTTAWA PA 280 ALBERT ST, SUITE 900, OTTAWA, ONTARIO K1P 5G8, CANADA SN 0008-4220 EI 1918-1833 J9 CAN J PLANT SCI JI Can. J. Plant Sci. PD JUN PY 2016 VL 96 IS 3 BP 321 EP 328 DI 10.1139/cjps-2015-0269 PG 8 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DP7OR UT WOS:000378689200001 ER PT J AU Bowen, P Shellie, KC Mills, L Willwerth, J Bogdanoff, C Keller, M AF Bowen, Pat Shellie, Krista C. Mills, Lynn Willwerth, Jim Bogdanoff, Carl Keller, Markus TI Abscisic acid form, concentration, and application timing influence phenology and bud cold hardiness in Merlot grapevines SO CANADIAN JOURNAL OF PLANT SCIENCE LA English DT Article DE grape; abscisic acid; bud hardiness; budbreak; differential thermal analysis; Vitis vinifera ID FREEZING TOLERANCE; LOW-TEMPERATURE; ACCLIMATION; ANALOGS AB The effects of abscisic acid (ABA) form, concentration, and application timing on bud cold hardiness, phenology, and fruiting performance of Merlot grapevines (Vitis vinifera) were evaluated in a three-year field trial with site locations in British Columbia and Ontario, Canada, and in Washington and Idaho, United States. Solutions containing natural S-ABA (ABA(N)) and a purported long-lived ABA analogue 8'-acetylene ABA (ABA(A)) at differing concentrations were applied to the vine canopy at veraison or post-harvest. Postharvest foliar applications of ABA(N) at concentrations greater than or equal to 5000 ppm tended to advance leaf abscission and increase autumn bud cold hardiness. Postharvest foliar applications of ABA(A) at 1000 ppm tended to delay budbreak in the spring following application and increase spring bud cold hardiness. Fruit yield and basic composition were affected little by the ABA treatments. Bud hardiness was enhanced by ABA(N) mostly in autumn and by ABAA mostly in the spring, indicating that the suitability of ABA forms for reducing bud damage would depend on when injurious cold events are more likely. C1 [Bowen, Pat; Bogdanoff, Carl] Summerland Res & Dev Ctr, Summerland, BC V0H 1Z0, Canada. [Shellie, Krista C.] USDA ARS, Hort Crops Res Unit, 29603 U I Lane, Parma, ID 83660 USA. [Mills, Lynn; Keller, Markus] Washington State Univ, IAREC, 24106 N Bunn Rd, Prosser, WA 99350 USA. [Willwerth, Jim] Brock Univ, Cool Climate Oenol & Viticulture Inst, St Catharines, ON L2S 3A1, Canada. RP Bowen, P (reprint author), Summerland Res & Dev Ctr, Summerland, BC V0H 1Z0, Canada. EM pat.bowen@agr.gc.ca FU Valent BioSciences; BC Wine Grape Council; Agriculture and Agri-Food Canada (DIAP); Agricultural Research Service Project [5358-21000-034] FX We gratefully acknowledge Valent BioSciences for their support of this project and for provision of abscisic acid. Funding from the BC Wine Grape Council and Agriculture and Agri-Food Canada (DIAP) is also acknowledged with gratitude. Funding was partially provided by the Agricultural Research Service Project 5358-21000-034 entitled "Production Systems to Promote Yield and Quality of Grapes in the Pacific Northwest". Excellent technical assistance was provided by Rob Brownlee, Steve Marsh, and Brad Estergaard in BC and Alan Muir and Monte Shields in ID. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that also may be suitable. NR 18 TC 1 Z9 1 U1 3 U2 7 PU AGRICULTURAL INST CANADA PI OTTAWA PA 280 ALBERT ST, SUITE 900, OTTAWA, ONTARIO K1P 5G8, CANADA SN 0008-4220 EI 1918-1833 J9 CAN J PLANT SCI JI Can. J. Plant Sci. PD JUN PY 2016 VL 96 IS 3 BP 347 EP 359 DI 10.1139/cjps-2015-0257 PG 13 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DP7OR UT WOS:000378689200004 ER PT J AU Coates, BS AF Coates, Brad S. TI Bacillus thuringiensis toxin resistance mechanisms among Lepidoptera: progress on genomic approaches to uncover causal mutations in the European corn borer, Ostrinia nubilalis SO CURRENT OPINION IN INSECT SCIENCE LA English DT Article ID FIELD-EVOLVED RESISTANCE; BRUSH-BORDER MEMBRANE; DELTA-ENDOTOXIN BINDING; MIDGUT AMINOPEPTIDASE N; HELIOTHIS-VIRESCENS; INSECT RESISTANCE; MANDUCA-SEXTA; HELICOVERPA-ARMIGERA; PINK-BOLLWORM; BT TOXINS AB Transgenic plants that express Bacillus thuringiensis (Bt) crystal (Cry) protein toxins (Bt crops) effectively control feeding by the European corn borer, Ostrinia nubilalis, although documented resistance evolution among a number of species in both the laboratory and field has heightened concerns about the durability of this technology. Research has provided major insights into the mutations that alter Bt toxin binding receptor structure and function within the midgut of Lepidoptera that directly impacts the efficacy of Bt toxins, and potentially leads to the evolution of resistance to Bt crops in the field. In this manuscript we provide an overview of available data on the identification of genes involved in high levels of resistance to Cry toxins, with emphasis on resistance described for O. nubilalis as the main target of Bt corn. C1 [Coates, Brad S.] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. RP Coates, BS (reprint author), USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. EM brad.coates@ars.usda.gov FU United States Department of Agriculture (USDA), Agricultural Research Service (ARS) (CRIS Project) [3625-22000-017-00]; Iowa Agriculture and Home Economics Experiment Station, Ames, IA [3543] FX This work was a joint contribution from the United States Department of Agriculture (USDA), Agricultural Research Service (ARS) (CRIS Project 3625-22000-017-00), and the Iowa Agriculture and Home Economics Experiment Station, Ames, IA (Project 3543). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the for its use. USDA is an equal opportunity provider and employer. NR 88 TC 0 Z9 0 U1 11 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2214-5745 EI 2214-5753 J9 CURR OPIN INSECT SCI JI Curr. Opin. Insect Sci. PD JUN PY 2016 VL 15 BP 70 EP 77 DI 10.1016/j.cois.2016.04.003 PG 8 WC Biology; Ecology; Entomology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Entomology GA DP7HY UT WOS:000378671000011 PM 27436734 ER PT J AU Finzel, JA Seyfried, MS Weltz, MA Launchbaugh, KL AF Finzel, Julie A. Seyfried, Mark S. Weltz, Mark A. Launchbaugh, Karen L. TI Simulation of long-term soil water dynamics at Reynolds Creek, Idaho: implications for rangeland productivity SO ECOHYDROLOGY LA English DT Article DE soil water; rangeland; sagebrush; model; SEM; plant yield ID UNITED-STATES; STREAMFLOW GENERATION; PLANT-COMMUNITIES; CLIMATE-CHANGE; MODEL; DATABASE; BALANCE; PRECIPITATION; STORAGE; ENVIRONMENT AB Plant productivity, forage availability and soil carbon dynamics are all strongly controlled by soil water in semi-arid rangelands. Sagebrush steppe ecosystems are among the most extensive in the western USA. We used the soil ecohydrology model (SEM) to simulate soil water dynamics and estimate plant growth at three different sagebrush steppe sites. SEM is a capacitance parameter model that uses a water balance approach to simulate daily changes in soil water and a modification of the de Wit equation to estimate plant yield. Model-simulated soil water results were evaluated using long-term (27-34years) measured data. We found that SEM accurately simulated soil water dynamics and total soil water storage at all three sites, with R-2 values greater than 0.8, Nash-Sutcliffe efficiencies near 0.8 and mean absolute errors of about 1.5cm. Model-estimated yield indices indicated a high degree of interannual variability, with values ranging more than fivefold and no long-term trend in plant production due to water availability. We also found that seasonal yield could be estimated with reasonable accuracy at the outset of the growing season (March 1) for about half of the years simulated due to either relatively high or low pre-growing season precipitation. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. Ecohydrology published by John Wiley & Sons, Ltd. C1 [Finzel, Julie A.] Univ Calif, UC Cooperat Extens, Bakersfield, CA USA. [Seyfried, Mark S.] ARS, USDA, Northwest Watershed Res Ctr, Boise, ID USA. [Weltz, Mark A.] ARS, USDA, Great Basin Rangeland Res Ctr, Reno, NV USA. [Launchbaugh, Karen L.] Univ Idaho, Moscow, ID 83843 USA. RP Weltz, MA (reprint author), ARS, USDA, 800 Pk Blvd, Boise, ID 83712 USA. EM mark.seyfried@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service; Natural Resource Conservation Service-Conservation Effects Assessment Project FX This research was funded by the United States Department of Agriculture, Agricultural Research Service and the Natural Resource Conservation Service-Conservation Effects Assessment Project. NR 59 TC 1 Z9 1 U1 8 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1936-0584 EI 1936-0592 J9 ECOHYDROLOGY JI Ecohydrology PD JUN PY 2016 VL 9 IS 4 BP 673 EP 687 DI 10.1002/eco.1666 PG 15 WC Ecology; Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA DP5PS UT WOS:000378550200010 ER PT J AU Campbell, JL Yanai, RD Green, MB Likens, GE See, CR Bailey, AS Buso, DC Yang, DQ AF Campbell, John L. Yanai, Ruth D. Green, Mark B. Likens, Gene E. See, Craig R. Bailey, Amey S. Buso, Donald C. Yang, Daqing TI Uncertainty in the net hydrologic flux of calcium in a paired-watershed harvesting study SO ECOSPHERE LA English DT Article DE calcium; error analysis; Hubbard Brook Experimental Forest; Monte Carlo; precipitation; Special Feature: Uncertainty Analysis; stream water; uncertainty; watershed; whole-tree harvest ID BROOK-EXPERIMENTAL-FOREST; HUBBARD-BROOK; NEW-HAMPSHIRE; STREAM-WATER; PRECIPITATION GAUGE; LOAD ESTIMATION; SOIL-NITROGEN; QUALITY DATA; CHEMISTRY; MODEL AB Monitoring solutes in precipitation inputs and stream water exports at small watersheds has greatly advanced our understanding of biogeochemical cycling. Surprisingly, although inputs to and outputs from ecosystems are instrumental to understanding sources and sinks of nutrients and other elements, uncertainty in these fluxes is rarely reported in ecosystem budgets. We illustrate error propagation in input-output budgets by comparing the net hydrologic flux of Ca in a harvested and reference watershed at the Hubbard Brook Experimental Forest, New Hampshire. We identify sources of uncertainty and use a Monte Carlo approach to combine many sources of uncertainty to produce an estimate of overall uncertainty. Sources of uncertainty in precipitation inputs included in this study were: rain gage efficiency (undercatch or overcatch), gaps in measurements of precipitation volume, selection of a model for interpolating among rain gages, unusable precipitation chemistry, and chemical analysis. Sources of uncertainty in stream water outputs were: stage height-discharge relationship, watershed area, gaps in the stream flow record, chemical analysis, and the selection of a method for flux calculation. The annual net hydrologic flux of Ca in the harvested and reference watersheds was calculated from 1973 through 2009. Relative to the reference watershed, the harvested watershed showed a marked increase in Ca flux after it was cut in 1983-1984, and slowly declined toward pretreatment levels thereafter. In 2009, the last year evaluated, the 95% confidence intervals for the annual estimates approach the 95% confidence intervals of the pretreatment regression line, suggesting that the increased net loss of Ca in the harvested watershed may soon be indistinguishable from the reference. Identifying the greatest sources of uncertainty can be used to guide improvements, for example in reducing instances of unusable precipitation chemistry and gaps in stream runoff. Our results highlight the value of estimating uncertainty in watershed studies, including those in which replication is impractical. C1 [Campbell, John L.; Green, Mark B.; Bailey, Amey S.] US Forest Serv, No Res Stn, USDA, Durham, NH 03824 USA. [Yanai, Ruth D.; See, Craig R.] SUNY Coll Environm Sci & Forestry, Dept Forest & Nat Resources Management, Syracuse, NY 13210 USA. [Green, Mark B.] Plymouth State Univ, Ctr Environm, Plymouth, NH 03264 USA. [Likens, Gene E.; Buso, Donald C.] Cary Inst Ecosyst Studies, Millbrook, NY 12545 USA. [Likens, Gene E.] Univ Connecticut, Dept Ecol & Evolutionary Biol, Storrs, CT 06269 USA. [Yang, Daqing] Environm Canada, Natl Hydrol Res Ctr, Saskatoon, SK S7N 3H5, Canada. RP Campbell, JL (reprint author), US Forest Serv, No Res Stn, USDA, Durham, NH 03824 USA. EM jlcampbell@fs.fed.us FU National Science Foundation (NSF) [DEB-1257906]; Long-Term Ecological Research (LTER) Network Office; NSF [DEB-1114804, DEB-1256696]; A.W. Mellon Foundation FX We thank Mary Martin for help with computer code. John Lombardi enhanced the efficiency of the program, and along with Brent Aulenbach and two anonymous reviewers, provided constructive comments on an earlier draft of this manuscript. This paper is a product of QUEST (Quantifying Uncertainty in Ecosystem Studies, funded as a Research Coordination Network by the National Science Foundation (NSF; DEB-1257906) and Long-Term Ecological Research (LTER) Network Office. QUEST supports uncertainty analysis by sharing papers, presentations, tutorials, and sample code (http://www.quantifyinguncertainty.org). This manuscript is a contribution of the Hubbard Brook Ecosystem Study. Hubbard Brook is part of the LTER network, which is supported by the NSF (DEB-1114804). Calcium data were obtained through funding to G.E. Likens from The A.W. Mellon Foundation and the NSF, including the LTER and Long Term Research in Environmental Biology (DEB-1256696) programs. Hydrologic data were provided by the USDA Forest Service. The Hubbard Brook Experimental Forest is operated and maintained by the USDA Forest Service, Northern Research Station, Newtown Square, Pennsylvania. NR 44 TC 0 Z9 0 U1 6 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD JUN PY 2016 VL 7 IS 6 AR e01299 DI 10.1002/ecs2.1299 PG 15 WC Ecology SC Environmental Sciences & Ecology GA DP5GA UT WOS:000378523100005 ER PT J AU LeBrun, JJ Thogmartin, WE Thompson, FR Dijak, WD Millspaugh, JJ AF LeBrun, Jaymi J. Thogmartin, Wayne E. Thompson, Frank R., III Dijak, William D. Millspaugh, Joshua J. TI Assessing the sensitivity of avian species abundance to land cover and climate SO ECOSPHERE LA English DT Article DE Avian abundance; Bayesian hierarchical models; Central USA; climate; Colinus virginianus; Helmitheros vermivorum; land cover; latitudinal gradient; North American Breeding Bird Survey; Setophaga discolor; Setophaga pinus; Vermivora cyanoptera ID PRAIRIE POTHOLE REGION; CONTERMINOUS UNITED-STATES; HABITAT LOSS; ADAPTATION STRATEGIES; LANDSCAPE FACTORS; POLEWARD SHIFTS; BIRDS; POPULATION; MODELS; BIODIVERSITY AB Climate projections for the Midwestern United States predict southerly climates to shift northward. These shifts in climate could alter distributions of species across North America through changes in climate (i.e., temperature and precipitation), or through climate-induced changes on land cover. Our objective was to determine the relative impacts of land cover and climate on the abundance of five bird species in the Central United States that have habitat requirements ranging from grassland and shrubland to forest. We substituted space for time to examine potential impacts of a changing climate by assessing climate and land cover relationships over a broad latitudinal gradient. We found positive and negative relationships of climate and land cover factors with avian abundances. Habitat variables drove patterns of abundance in migratory and resident species, although climate was also influential in predicting abundance for some species occupying more open habitat (i.e., prairie warbler, blue-winged warbler, and northern bobwhite). Abundance of northern bobwhite increased with winter temperature and was the species exhibiting the most significant effect of climate. Models for birds primarily occupying early successional habitats performed better with a combination of habitat and climate variables whereas models of species found in contiguous forest performed best with land cover alone. These varied species-specific responses present unique challenges to land managers trying to balance species conservation over a variety of land covers. Management activities focused on increasing forest cover may play a role in mitigating effects of future climate by providing habitat refugia to species vulnerable to projected changes. Conservation efforts would be best served focusing on areas with high species abundances and an array of habitats. Future work managing forests for resilience and resistance to climate change could benefit species already susceptible to climate impacts. C1 [LeBrun, Jaymi J.; Millspaugh, Joshua J.] Univ Missouri, Dept Fisheries & Wildlife Sci, 302 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. [Thogmartin, Wayne E.] US Geol Survey, Upper Midwest Environm Sci Ctr, 2630 Fanta Reed Rd, La Crosse, WI 54603 USA. [Thompson, Frank R., III; Dijak, William D.] Univ Missouri, USDA Forest Serv, No Res Stn, 202 Anheuser Busch Nat Resources, Columbia, MO 65211 USA. RP LeBrun, JJ (reprint author), Univ Missouri, Dept Fisheries & Wildlife Sci, 302 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. EM jjl59a@mail.missouri.edu RI Thogmartin, Wayne/A-4461-2008 OI Thogmartin, Wayne/0000-0002-2384-4279 FU USDA Forest Service Northern Research Station; Department of Interior Northeast Climate Science Center graduate fellowship FX We thank the many volunteers collecting North American Breeding Bird Survey counts, without whose efforts this study would not have been possible. Funding for this work was provided by the USDA Forest Service Northern Research Station and a Department of Interior Northeast Climate Science Center graduate fellowship. Any use of trade, product, or firm names are for descriptive purposes only and do not imply endorsement by the U.S. Government. Contents of the manuscript are solely the responsibility of the authors and do not necessarily represent views of the U.S. Government. This manuscript is submitted for publication with the understanding that the U.S. Government is authorized to reproduce and distribute reprints for Governmental purposes. NR 96 TC 0 Z9 0 U1 16 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD JUN PY 2016 VL 7 IS 6 AR e01359 DI 10.1002/ecs2.1359 PG 22 WC Ecology SC Environmental Sciences & Ecology GA DP5GA UT WOS:000378523100023 ER PT J AU Loudermilk, EL Hiers, JK Pokswinski, S O'Brien, JJ Barnett, A Mitchell, RJ AF Loudermilk, E. Louise Hiers, J. Kevin Pokswinski, Scott O'Brien, Joseph J. Barnett, Analie Mitchell, Robert J. TI The path back: oaks (Quercus spp.) facilitate longleaf pine (Pinus palustris) seedling establishment in xeric sites SO ECOSPHERE LA English DT Article DE deciduous oaks; Eglin Air Force Base; Kaplan-Meier; longleaf pine; management; plant facilitation; regression trees; restoration; seed masting; turkey oak; xeric sandhill ID HARDWOOD REDUCTION TECHNIQUES; NORTHWEST FLORIDA; PLANT-COMMUNITIES; PRESCRIBED FIRE; HYDRAULIC LIFT; UNITED-STATES; TREE; FOREST; OVERSTORY; ECOSYSTEMS AB Understanding plant-plant facilitation is critical for predicting how plant community function will respond to changing disturbance and climate. In longleaf pine (Pinus palustris Mill.) ecosystems of the southeastern United States, understanding processes that affect pine reproduction is imperative for conservation efforts that aim to maintain ecosystem resilience across its wide geographic range and edaphic gradients. Variation in wildland fire and plant-plant interactions may be overlooked in " coarse filter" restoration management, where actions are often prescribed over a variety of ecological conditions with an assumed outcome. For example, hardwood reduction techniques are commonly deemed necessary for ecological restoration of longleaf pine ecosystems, as hardwoods are presumed competitors with longleaf pine seedlings. Natural regeneration dynamics are difficult to test experimentally given the infrequent and irregular mast seed events of the longleaf pine. Using a long-term, large-scale restoration experiment and a long-term monitoring data site at Eglin Air Force Base, Florida (USA), this study explores the influence of native fire-intolerant oaks on longleaf regeneration. We test for historical observations of hardwood facilitation against the null hypothesis of competitive exclusion. Our results provide evidence of hardwood facilitation on newly germinated longleaf pine seedlings (<2 yr old) after two mast seeding events (1996, 2011). Using regression-tree and Kaplan-Meier survival analyses, we found that deciduous oak midstory density was the most significant variable associated with longleaf pine seedling survival rates in the first 2 yr after germination. We found that as few as 43 oak midstory stems ha(-1) were sufficient to facilitate seedling survival, but as many as 1400 stems ha-1 maintained facilitation without competitive exclusion of seedlings. We found that 1.5-yr-old pine seedlings were more moisture stressed under more open canopy conditions when compared to those immediately adjacent to a midstory oak canopy. Recognition that deciduous oaks are important facilitators of longleaf seedling establishment on xeric sites represents a significant departure from conventional wisdom and current management practices that has largely focused on competitive exclusion. This points to a critical role of a deciduous oak midstory of moderate densities for long-term ecosystem resilience in xeric longleaf pine ecosystems in light of climate uncertainty. C1 [Loudermilk, E. Louise; O'Brien, Joseph J.] US Forest Serv, Ctr Forest Disturbance Sci, Southern Res Ctr, 320 Green St, Athens, GA 30602 USA. [Hiers, J. Kevin] Univ South, Off Environm Stewardship, 735 Univ Ave, Sewanee, TN 37383 USA. [Pokswinski, Scott] Univ Nevada, 1664 N Virginia St, Reno, NV 89503 USA. [Barnett, Analie] Nature Conservancy, 100 Peachtree St NW, Atlanta, GA 30303 USA. [Mitchell, Robert J.] Joseph W Jones Ecol Res Ctr, Route 2,Box 2324, Newton, GA 31770 USA. RP Loudermilk, EL (reprint author), US Forest Serv, Ctr Forest Disturbance Sci, Southern Res Ctr, 320 Green St, Athens, GA 30602 USA. EM elloudermilk@fs.fed.us FU Strategic Environmental Research and Development Program [RC-1696, RC-2243]; Forestry Sciences Laboratory of the USDA Forest Service, Athens, GA FX The field work and writing was funded in part by the Strategic Environmental Research and Development Program (projects RC-1696 and RC-2243). We thank the Forestry Sciences Laboratory of the USDA Forest Service, Athens, GA for their support. We thank Jackson Guard and, in particular, Brett Williams, at Eglin Air Force Base for their support. Bob Mitchell (co-author) was at the heart of this paper, providing much of the ideas and forethought. Bob continuously provided insight in the study of longleaf pine ecology, restoration, and management and will be greatly missed by his colleagues and friends. NR 68 TC 0 Z9 0 U1 20 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD JUN PY 2016 VL 7 IS 6 AR e01361 DI 10.1002/ecs2.1361 PG 14 WC Ecology SC Environmental Sciences & Ecology GA DP5GA UT WOS:000378523100025 ER PT J AU Wallingford, AK Lee, JC Loeb, GM AF Wallingford, Anna K. Lee, Jana C. Loeb, Gregory M. TI The influence of temperature and photoperiod on the reproductive diapause and cold tolerance of spotted-wing drosophila, Drosophila suzukii SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article DE Diptera; Drosophilidae; invasive pest; token stimuli; diapause induction; diapause termination; egg load; chill coma recovery rate; cold stress ID DIPTERA DROSOPHILIDAE; TIME MEASUREMENT; UNITED-STATES; OVARIAN DIAPAUSE; FRUIT PRODUCTION; INSECT DIAPAUSE; MELANOGASTER; INDUCTION; INFESTATION; CYCLES AB Knowledge regarding the reproductive status of spotted-wing drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), is of critical importance in predicting potential infestations of this invasive pest, as eggs are laid in ripe or ripening fruit of several commercially important small-fruit crops. Token environmental stimuli for the induction of reproductive diapause and improved cold tolerance were identified for D.suzukii. Diapause induction was evaluated by assessing, via dissection, the number of mature eggs carried by field-captured D.suzukii and laboratory-reared D.suzukii held under various temperature and photoperiod regimes. Egg load decreased over time in females trapped from July to December at sites in Benton County, OR, and Ontario County, NY, both USA, and reached zero eggs by December at all sites. Photoperiods below 14h of day length led to reduced egg maturation in laboratory-reared flies held at moderate temperatures (15 or 20 degrees C). Whereas very few mature eggs were found in females held at 10 degrees C under short- or long-day photoperiods for several weeks after eclosion, a spontaneous return to ovarian maturity was observed in short-day-entrained females after 7weeks. Diapause termination was investigated by evaluatingfecundity in diapausing females returned to optimal environmental conditions. Whereas long-day-entrained flies began producing offspring immediately upon return to optimal conditions, short-day-entrained flies returned after 1 and 6weeks at 10 degrees C were slower to produce offspring than colony flies or short-day-entrained flies returned after 7weeks. Cold tolerance was evaluated by observing chill coma recovery rates after 24h exposure to -1 degrees C. Cold-acclimated (diapausing) females recovered from chill coma faster than cold-hardened or unacclimated females. C1 [Wallingford, Anna K.; Loeb, Gregory M.] Cornell Univ, New York Agr Expt Stn, 630 W North St, Geneva, NY 14456 USA. [Lee, Jana C.] ARS, USDA, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. RP Wallingford, AK (reprint author), Cornell Univ, New York Agr Expt Stn, 630 W North St, Geneva, NY 14456 USA. EM akw52@cornell.edu FU Cornell University's New York State Agricultural Experiment Station Federal Formula Funds [2012-13-195]; NYS Dept. of Agriculture & Markets Specialty Crops Block Grant [C200783]; NYS Farm Viability Initiative [FOC 14 001]; USDA SCRI Grant [2010-51181-21167]; USDA CRIS [5358-22000-037-00D] FX We would like to thank Gabrielle Brind'Amour, Alison Wentworth, Steve Hesler, and Molly Albrecht for their assistance with field collections and laboratory bioassays. Thanks to Joanna Chui and two anonymous reviewers who provided insightful suggestions for improvement to the manuscript. Thanks to Erika Mudrak for her assistance with statistical analysis. This research was supported in part by funding from Cornell University's New York State Agricultural Experiment Station Federal Formula Funds, project # 2012-13-195, NYS Dept. of Agriculture & Markets Specialty Crops Block Grant #C200783, and NYS Farm Viability Initiative # FOC 14 001 to GL, as well as from USDA SCRI Grant 2010-51181-21167, and USDA CRIS 5358-22000-037-00D to JL. NR 65 TC 2 Z9 2 U1 13 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0013-8703 EI 1570-7458 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD JUN PY 2016 VL 159 IS 3 BP 327 EP 337 DI 10.1111/eea.12443 PG 11 WC Entomology SC Entomology GA DP6VN UT WOS:000378637400005 ER PT J AU Dodds, KJ Hanavan, RP Wansleben, T AF Dodds, Kevin J. Hanavan, Ryan P. Wansleben, Tom TI Enhancing Stand Structure through Snag Creation in Northeastern US Forests: Using Ethanol Injections and Bark Beetle Pheromones to Artificially Stress Red Maple and White Pine SO FORESTS LA English DT Article DE coarse woody debris; tree stress; tree colonization; tree fitness; tree vitality; arboreal beetles ID COARSE WOODY DEBRIS; COLEOPTERA-CURCULIONIDAE SCOLYTINAE; SOUTHEASTERN UNITED-STATES; NORTHERN HARDWOOD FORESTS; AMBROSIA BEETLES; SIREX-NOCTILIO; TRAP TREES; XYLOSANDRUS-GERMANUS; WESTERN OREGON; DOUGLAS-FIR AB We investigated two methods to create white pine and red maple snags in a forested setting. The first involved injecting trees with ethanol at two times (single Ethanol (ETOH) and double ETOH injections) to increase attractiveness to insects and elicit attacks on trees. The second method was unique to white pines and involved both injection treatments in combination with baiting trees with Ips-specific pheromones. Three of five white pines from the double ETOH treatment died in the second year. Species including Ips pini (Say), Ips grandicollis Eichhoff, Orthotomicus caelatus Eichhoff, Crypturgus borealis Swaine and Monochamus notatus (Drury) responded more strongly to at least one of the treatments over control trees. However, there were no differences found in individual Scolytinae or Cerambycidae species response to treatments in red maple. Fitness (FV/FM) and vitality (PIabs) were both significantly reduced in both ETOH treatments compared to controls in white pine. In red maple, fitness was reduced in the double ETOH treated trees but the final mean FV/FM values were within the approximate optimal of health. Ethanol injections, in combination with Ips-specific semiochemicals, show promise for creating standing coarse woody debris (CWD) in white pine. Injecting ethanol was not effective for stressing red maple. C1 [Dodds, Kevin J.; Hanavan, Ryan P.] US Forest Serv, Northeastern Area State & Private Forestry, 271 Mast Rd, Durham, NH 03824 USA. [Wansleben, Tom] Mt Grace Land Conservat Trust, 1461 Old Keene Rd, Athol, MA 01331 USA. RP Dodds, KJ (reprint author), US Forest Serv, Northeastern Area State & Private Forestry, 271 Mast Rd, Durham, NH 03824 USA. EM kdodds@fs.fed.us; rphanavan@fs.fed.us; thomas.wansleben@state.ma.us NR 67 TC 0 Z9 0 U1 7 U2 7 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD JUN PY 2016 VL 7 IS 6 AR 124 DI 10.3390/f7060124 PG 15 WC Forestry SC Forestry GA DP9XT UT WOS:000378852000015 ER PT J AU Hatcher, JE Straka, TJ Cushing, TL Greene, JL Bridges, WC AF Hatcher, John E., Jr. Straka, Thomas J. Cushing, Tamara L. Greene, John L. Bridges, William C., Jr. TI Socioeconomic Predictors of Family Forest Owner Awareness and Use of US Federal Income Tax Provisions SO FORESTS LA English DT Article DE federal income tax; family forest; capital gains tax; seven-year amortization ID INCENTIVE PROGRAMS; UNITED-STATES; MANAGEMENT; LANDOWNERS; INVESTMENTS; BEHAVIOR; IMPACT; SOUTH; ACT AB Family forest owners (FFOs) control a majority of private forestland in the United States and have widely diverse ownership and management objectives. Many FFOs manage their holdings for timber production and, thus, are concerned with issues such as reforestation incentives and tax treatment of timber revenues. Their actual knowledge of the tax aspects of timber management varies, with some owners even unaware of the federal income tax provisions that apply to timber. This research used econometric techniques to establish socioeconomic predictors of FFO awareness and use of federal income tax provisions. Socioeconomic factors (such as size of forest holding, ownership objective, education, age, and income) were evaluated in terms of association with awareness and use of income tax provisions. Data were obtained from a survey of 1350 South Carolina FFOs (472 useable responses). A two-step sample selection methodology revealed that membership in a landowner organization and size of forest holding positively influence landowner awareness of the seven tax provisions, while ownership objective and level of education exhibited varying degrees of influence. Overall, the findings suggest that size of forest holding is the key determinant that influences landowner use of the provisions. These tax incentives are one of the foundations of federal policies encouraging active forest management by FFOs and the effectiveness of the various incentives has crucial implications for forest policy analysis. C1 [Hatcher, John E., Jr.; Straka, Thomas J.] Clemson Univ, Dept Forestry & Environnemental Conservat, Box 340317, Clemson, SC 29634 USA. [Cushing, Tamara L.] Oregon State Univ, Dept Forest Engn Resources & Management, 203 Peavy Hall, Corvallis, OR 97331 USA. [Greene, John L.] US Forest Serv, USDA, POB 12254, Res Triangle Pk, NC 27709 USA. [Bridges, William C., Jr.] Clemson Univ, Dept Math Sci, Box 340975, Clemson, SC 29634 USA. RP Straka, TJ (reprint author), Clemson Univ, Dept Forestry & Environnemental Conservat, Box 340317, Clemson, SC 29634 USA. EM tstraka@clemson.edu; Tamara.Cushing@oregonstate.edu; johnlgreene@fs.fed.us; wbrdgs@clemson.edu FU USDA Forest Service, Southern Research Station FX This research was funded by the USDA Forest Service, Southern Research Station. NR 65 TC 0 Z9 0 U1 1 U2 1 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD JUN PY 2016 VL 7 IS 6 AR 114 DI 10.3390/f7060114 PG 15 WC Forestry SC Forestry GA DP9XT UT WOS:000378852000005 ER PT J AU Wang, WW Page-Dumroese, D Lv, RH Xiao, C Li, GL Liu, Y AF Wang, Weiwei Page-Dumroese, Deborah Lv, Ruiheng Xiao, Chen Li, Guolei Liu, Yong TI Soil Enzyme Activities in Pinus tabuliformis (Carriere) Plantations in Northern China SO FORESTS LA English DT Article DE invertase; urease; catalase; phenol oxidase; forest thinning ID MICROBIAL BIOMASS; LITTER DECOMPOSITION; ORGANIC-MATTER; NITROGEN DEPOSITION; FOREST SOIL; CARBON; COMMUNITIES; INDICATORS; EUCALYPTUS; QUALITY AB Changes in forest stand structure may alter the activity of invertase, urease, catalase and phenol oxidase after thinning Pinus tabuliformis (Carriere) plantations in Yanqing County of Beijing, China. We examined changes in these soil enzymes as influenced by time since thinning (24, 32, and 40 years since thinning) for 3 seasons (spring, summer and autumn) following harvesting at two depths in the mineral soil (0-10 cm and 10-20 cm). Invertase and urease increased significantly with time since thinning. Catalase activity was highest in the 24-year-old stand and there were no statistically significant differences between the 32-and 40-year-old stands. In addition, maximum invertase, urease, catalase, and phenol oxidase activities occurred during the summer; minimum activities occurred in autumn. Invertase and urease were positively correlated with each other, as were catalase and phenol oxidase. Most soil enzyme activity was higher in the 0-10 cm layer than at the 10-20 cm depth. As time from thinning increased, differences among soil depth became less significant. These results suggest that seasonal changes of these enzymes have different roles, as the time since thinning and thinning treatments may have both short-and long-term impacts on soil microbial activity. C1 [Wang, Weiwei; Xiao, Chen; Li, Guolei; Liu, Yong] Beijing Forestry Univ, Key Lab Silviculture & Conservat, Minist Educ, Beijing 100083, Peoples R China. [Page-Dumroese, Deborah] US Forest Serv, USA Dept Agr, Rocky Mt Res Stn, 1221 South Main St, Moscow, ID 83843 USA. [Lv, Ruiheng] Tarim Univ, Coll Plant Sci & Technol, Alar Xinjiang 843300, Peoples R China. RP Liu, Y (reprint author), Beijing Forestry Univ, Key Lab Silviculture & Conservat, Minist Educ, Beijing 100083, Peoples R China. EM weiweiwang8893@gmail.com; ddumroese@fs.fed.us; Ivrh514723@126.com; chenxiao_0123@126.com; glli226@163.com; lyong@bjfu.edu.cn FU National Natural Science Foundation of China [30972353] FX This work was supported by the National Natural Science Foundation of China (No. 30972353). NR 72 TC 0 Z9 0 U1 13 U2 20 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD JUN PY 2016 VL 7 IS 6 AR 112 DI 10.3390/f7060112 PG 12 WC Forestry SC Forestry GA DP9XT UT WOS:000378852000003 ER PT J AU Park, KE Park, CH Powell, A Martin, J Donovan, DM Telugu, BP AF Park, Ki-Eun Park, Chi-Hun Powell, Anne Martin, Jessica Donovan, David M. Telugu, Bhanu P. TI Targeted Gene Knockin in Porcine Somatic Cells Using CRISPR/Cas Ribonucleoproteins SO INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES LA English DT Article DE CRISPR/Cas; SCNT; cloning; knockin; TARGATT pig; transegenic ID HOMOLOGY-DIRECTED REPAIR; CYSTIC-FIBROSIS; MOUSE MODELS; INTEGRASE; GENERATION; EFFICIENCY; PIGS AB The pig is an ideal large animal model for genetic engineering applications. A relatively short gestation interval and large litter size makes the pig a conducive model for generating and propagating genetic modifications. The domestic pig also shares close similarity in anatomy, physiology, size, and life expectancy, making it an ideal animal for modeling human diseases. Often, however, the technical difficulties in generating desired genetic modifications such as targeted knockin of short stretches of sequences or transgenes have impeded progress in this field. In this study, we have investigated and compared the relative efficiency of CRISPR/Cas ribonucleoproteins in engineering targeted knockin of pseudo attP sites downstream of a ubiquitously expressed COL1A gene in porcine somatic cells and generated live fetuses by somatic cell nuclear transfer (SCNT). By leveraging these knockin pseudo attP sites, we have demonstrated subsequent phiC31 integrase mediated integration of green fluorescent protein (GFP) transgene into the site. This work for the first time created an optimized protocol for CRISPR/Cas mediated knockin in porcine somatic cells, while simultaneously creating a stable platform for future transgene integration and generating transgenic animals. C1 [Park, Ki-Eun; Park, Chi-Hun; Martin, Jessica; Telugu, Bhanu P.] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. [Park, Ki-Eun; Park, Chi-Hun; Powell, Anne; Martin, Jessica; Donovan, David M.; Telugu, Bhanu P.] ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD 20705 USA. [Park, Ki-Eun; Telugu, Bhanu P.] Livestock Genomix, Reisterstown, MD 21136 USA. RP Telugu, BP (reprint author), Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA.; Telugu, BP (reprint author), ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD 20705 USA.; Telugu, BP (reprint author), Livestock Genomix, Reisterstown, MD 21136 USA. EM kepark@umd.edu; chpark@umd.edu; anne.powell@ars.usda.gov; jjm11393@umd.edu; david.donovan@ars.usda.gov; btelugu@umd.edu OI Park, Chi-Hun/0000-0002-7618-9028 FU Genus plc; National Institute of Food and Agriculture (NIFA) [2015-67015-22845]; ARS Project [8042-31000-103-00D] FX We thank Agricultural Research Station (ARS) staff and attending veterinarian for help with animal work. Bhanu P. Telugu is supported by Genus plc and National Institute of Food and Agriculture (NIFA) Dual purpose with Dual Benefit Grant #2015-67015-22845. David M. Donovan and Anne Powell are supported by ARS Project #8042-31000-103-00D. NR 26 TC 2 Z9 2 U1 8 U2 18 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1422-0067 J9 INT J MOL SCI JI Int. J. Mol. Sci. PD JUN PY 2016 VL 17 IS 6 AR 810 DI 10.3390/ijms17060810 PG 11 WC Biochemistry & Molecular Biology; Chemistry, Multidisciplinary SC Biochemistry & Molecular Biology; Chemistry GA DP9EK UT WOS:000378799300024 ER PT J AU Wilk, RJ Raphael, MG Bloxton, TD AF Wilk, Randall J. Raphael, Martin G. Bloxton, Thomas D., Jr. TI Nesting habitat characteristics of Marbled Murrelets occurring in near-shore waters of the Olympic Peninsula, Washington SO JOURNAL OF FIELD ORNITHOLOGY LA English DT Article DE Brachyramphus marmoratus; Juan de Fuca; old-growth conifer forest; Pacific Northwest; seabird; threatened species; Vancouver Island ID SOUTHEAST ALASKA; BREEDING SUCCESS; MAINLAND FJORD; LANDSCAPES; XANTUS; OREGON; RADAR AB Marbled Murrelets (Brachyramphus marmoratus) are listed as threatened in the portion of their range extending from British Columbia to California due to loss of nesting habitat. Recovery of Marbled Murrelet populations requires a better understanding of the characteristics of their nesting habitat in this part of their range. Our objective, therefore, was to describe their nesting habitat in Washington State and Vancouver Island, British Columbia. We captured Marbled Murrelets from 2004 to 2008, fitted them with radio transmitters, and followed them to nests (N = 20). We used Cohen's unbiased d effect size to assess differences between forest plots surrounding nest sites and nearby control sites (N = 18). Nest sites had less canopy cover of the dominant conifers and fewer, but larger, trees than control sites. Nest sites also had greater percentages of trees with platforms >10 cm diameter and >15 cm diameter, and more platforms of these sizes than control sites. The mean diameter at breast height of nest trees was 136.5 cm (range = 84-248 cm) and all but one nest was in dominant or co-dominant tree species. At the landscape scale, we used vegetation maps derived from remotely sensed data and found greater canopy cover, higher density of mature trees, more platforms >10 cm/ha, and more old-growth habitat at nest sites than at random sites. Our findings suggest that, at the site scale, nesting Marbled Murrelets selected the most suitable features of forest structure across expansive potentially suitable habitat. Our landscape-scale analysis showed that habitat features in nesting stands differed from those features in available stands in the murrelet's range in Washington. We also found that stands with nests were less fragmented than available forest across murrelet range. All nest sites of radio-tagged birds in Washington were in protected areas in mostly undisturbed forest habitat. Conservation of these areas of inland nesting habitat will be critical to the recovery of Marbled Murrelet populations. C1 [Wilk, Randall J.; Raphael, Martin G.; Bloxton, Thomas D., Jr.] US Forest Serv, Forestry Sci Lab, USDA, Pacific NW Res Stn, 3625 93rd Ave SW, Olympia, WA 98512 USA. RP Wilk, RJ (reprint author), US Forest Serv, Forestry Sci Lab, USDA, Pacific NW Res Stn, 3625 93rd Ave SW, Olympia, WA 98512 USA. EM rwilk@fs.fed.us FU USDA Forest Service; Pacific Northwest Research Station; Washington Department of Natural Resources; Olympic Natural Resources Center; USDI Fish and Wildlife Service FX A. Barna, M. Comer, A. Havron, D. Evans Mack, and at least nine other researchers helped collect habitat data. N. Hatch, P. Horne, A. Horton, S. Horton, D. McGraw, D. Ramos, and C. VanStratt were directly involved in data collection at nest sites and radio-tagging in multiple years. J. Adams, N. Parker, and Z. Peery provided training for at-sea endeavors. Rite Brothers Aviation conducted all tracking flights. Simon Fraser University, British Columbia Ministry of Environment, University of Victoria, University of Washington, College of Forest Resources, U.S. Fish and Wildlife Service, Washington Department of Fish and Wildlife, Sandpiper Technologies, Inc., Olympic National Park, Olympic National Forest, and Mt. Baker-Snoqualmie National Forest provided logistical support. Funding was provided by the USDA Forest Service, Pacific Northwest Research Station, Washington Department of Natural Resources, Olympic Natural Resources Center, and the USDI Fish and Wildlife Service. R. Bigley, P. Harrison, and D. Lynch helped secure additional funding from outside sources. A. Shirk produced the Fragstats data. E. Steel conducted the statistical review of the early manuscript. Suggestions by an anonymous statistics reviewer, anonymous reviewers, B. Barbaree, and T. Lorenz greatly improved this manuscript. NR 43 TC 1 Z9 1 U1 4 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0273-8570 EI 1557-9263 J9 J FIELD ORNITHOL JI J. Field Ornithol. PD JUN PY 2016 VL 87 IS 2 BP 162 EP 175 DI 10.1111/jofo.12150 PG 14 WC Ornithology SC Zoology GA DP6SK UT WOS:000378628900005 ER PT J AU Lind, AJ Welsh, HH Wheeler, CA AF Lind, Amy J. Welsh, Hartwell H., Jr. Wheeler, Clara A. TI Foothill Yellow-Legged Frog (Rana boylii) Oviposition Site Choice at Multiple Spatial Scales SO JOURNAL OF HERPETOLOGY LA English DT Article ID POPULATION-DYNAMICS; HABITAT SELECTION; RIVER; CONSERVATION; CALIFORNIA; ANURAN; METAPOPULATION; LANDSCAPES; STRATEGIES; FIDELITY AB Studies of resource selection at multiple scales are critical to understanding ecological and evolutionary attributes of a species. We analyzed relative abundance, habitat use, and oviposition site selection of Foothill Yellow-Legged Frogs (Rana boylii) at 11 localities across two geographic regions in California (northern Coast Range and Sierra Nevada) over 16 yr. We found narrow ranges for oviposition microhabitat characteristics (water depth, water velocity, and stream substrate) among study localities. At the Main and South forks of the Trinity River, variances of the habitat traits were lower for oviposition microsites than for random points within breeding areas, indicating fine-scale selection. On the South Fork Trinity, egg mass relative abundances were negatively associated with water depth and positively associated with distance from the shoreline, suggesting that breeding areas with high egg mass relative abundances generally occurred in wide shallow areas. We observed long-term repeated use of breeding sites. At the South Fork Trinity, 63% of potentially suitable breeding areas were used consecutively for 3 yr, and at Hurdygurdy Creek several areas were used in >= 11 yr. Oviposition site selection and microhabitat specificity may result in population stability even within the substantial temporal and spatial variability of stream environments. Management of stream environments and conservation plans for R. boylii could benefit by preserving hydrologic processes that produce these specific habitats and identifying and protecting high-use breeding areas. C1 [Lind, Amy J.] US Forest Serv, USDA, Pacific Southwest Res Stn, Sierra Nevada Res Ctr, 2121 Second St,Suite A-101, Davis, CA 95618 USA. [Welsh, Hartwell H., Jr.; Wheeler, Clara A.] US Forest Serv, USDA, Pacific Southwest Res Stn, Redwood Sci Lab, 1700 Bayview Dr, Arcata, CA 95521 USA. [Lind, Amy J.] US Forest Serv, USDA, Tahoe & Plumas Natl Forests, 631 Coyote St, Nevada City, CA 95959 USA. RP Wheeler, CA (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, Redwood Sci Lab, 1700 Bayview Dr, Arcata, CA 95521 USA. EM cawheeler@fs.fed.us FU U.S. Department of the Interior Bureau of Reclamation; U.S. Department of Agriculture Forest Service; Region 5 Fish Habitat Relationships Program; University of California, Davis FX We thank J. Dahl, G. Hodgson, S. Mook, K. Shimizu, and R. Wilson for help with data collection in northwestern California. L. Conway, H. Sanders, Pacific Gas and Electric Company, and T. Van Wagner contributed data for the Sierra Nevada. G. Hodgson and B. Hogoboom helped with analysis and figures and D. Montoya generated watershed area parameters. J. Baldwin provided statistical guidance and T. Beebee, S. Greco, P. Moyle, H. B. Shaffer, S. Yarnell, and two anonymous reviewers provided helpful reviews of earlier drafts of this paper. Funding for some of this work was provided by the U.S. Department of the Interior Bureau of Reclamation, U.S. Department of Agriculture Forest Service, Region 5 Fish Habitat Relationships Program, and University of California, Davis grants (to AJL). NR 48 TC 1 Z9 1 U1 5 U2 6 PU SOC STUDY AMPHIBIANS REPTILES PI ST LOUIS PA C/O ROBERT D ALDRIDGE, ST LOUIS UNIV, DEPT BIOLOGY, 3507 LACLEDE, ST LOUIS, MO 63103 USA SN 0022-1511 EI 1937-2418 J9 J HERPETOL JI J. Herpetol. PD JUN PY 2016 VL 50 IS 2 BP 263 EP 270 DI 10.1670/14-169 PG 8 WC Zoology SC Zoology GA DP8RX UT WOS:000378766500013 ER PT J AU Youn, UJ Park, EJ Kondratyuk, TP Sang-Ngern, M Wall, MM Wei, YZ Pezzuto, JM Chang, LC AF Youn, Ui Joung Park, Eun-Jung Kondratyuk, Tamara P. Sang-Ngern, Mayuramas Wall, Marisa M. Wei, Yanzhang Pezzuto, John M. Chang, Leng Chee TI Anti-inflammatory and Quinone Reductase Inducing Compounds from Fermented Noni (Morinda citrifolia) Juice Exudates SO JOURNAL OF NATURAL PRODUCTS LA English DT Article ID IRIDOID GLYCOSIDES; CHEMICAL-CONSTITUENTS; STRUCTURE ELUCIDATION; KAPPA-B; CANCER; FRUITS; ANTHRAQUINONE; GLUCOSIDES; LEAVES; HEPATOTOXICITY AB A new fatty acid ester disaccharide, 2-O-(beta-D-glucopyranosyl)-1-O-(2E,4Z,7Z)-deca-2,4,7-trienoyl-beta-D-glucopyranose (1), a new ascorbic acid derivative, 2-caffeoyl-3-ketohexulofuranosonic acid gamma-lactone (2), and a new iridoid glycoside, 10-dimethoxyfermiloside (3), were isolated along with 13 known compounds (4-16) from fermented noni fruit juice (Morinda citrifolia). The structures of the new compounds, together with 4 and 5, were determined by 1D and 2D NMR experiments, as well as comparison with published values. Compounds 2 and 7 showed moderate inhibitory activities in a TNF-alpha-induced NF-kappa B assay, and compounds 4 and 6 exhibited considerable quinone reductase-1 (QR1) inducing effects. C1 [Youn, Ui Joung; Park, Eun-Jung; Kondratyuk, Tamara P.; Sang-Ngern, Mayuramas; Pezzuto, John M.; Chang, Leng Chee] Univ Hawaii, Daniel K Inouye Coll Pharm, Dept Pharmaceut Sci, Hilo, HI 96720 USA. [Youn, Ui Joung] KIOST, Korea Polar Res Inst, Div Life Sci, Inchon 21990, South Korea. [Wall, Marisa M.] Univ Hawaii, USDA, Daniel K Inouye US Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. [Wei, Yanzhang] Clemson Univ, Clemson, SC 29634 USA. [Pezzuto, John M.] Long Isl Univ, Arnold & Marie Schwartz Coll Pharm & Hlth Sci, Brooklyn, NY 11201 USA. RP Chang, LC (reprint author), Univ Hawaii, Daniel K Inouye Coll Pharm, Dept Pharmaceut Sci, Hilo, HI 96720 USA. EM lengchee@hawaii.edu FU UH-Hilo (Daniel K. Inouye College of Pharmacy); NCI Program Project [P01CA48112]; Hawaii Community Foundation; NCRR INBRE Program [P20 RR016467]; Korea Polar Research Institute, KOPRI [PM15050] FX We thank UH-Hilo (Daniel K. Inouye College of Pharmacy) for start-up funding. This research was supported by NCI Program Project P01CA48112 (to J.M.P.), Hawaii Community Foundation, and NCRR INBRE Program P20 RR016467 (to L.C.C.). This work was also supported by a grant to the Korea Polar Research Institute, KOPRI, under project PM15050. We thank H. S. Shin, National Center for Inter University Research Facilities, Seoul National University, for access to the Mass Spectrometry Facility used in this study. NR 43 TC 1 Z9 1 U1 8 U2 13 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0163-3864 EI 1520-6025 J9 J NAT PROD JI J. Nat. Prod. PD JUN PY 2016 VL 79 IS 6 BP 1508 EP 1513 DI 10.1021/acs.jnatprod.5b00970 PG 6 WC Plant Sciences; Chemistry, Medicinal; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy GA DP8OT UT WOS:000378758200004 PM 27196335 ER PT J AU Weber, JG Burnett, JW Xiarchos, IM AF Weber, Jeremy G. Burnett, J. Wesley Xiarchos, Irene M. TI Broadening Benefits from Natural Resource Extraction: Housing Values and Taxation of Natural Gas Wells as Property SO JOURNAL OF POLICY ANALYSIS AND MANAGEMENT LA English DT Article ID SHALE GAS; MARKET IMPACTS; COAL BOOM; PROGRAM; TAXES; MODEL; BUST; EDUCATION; DEMAND; INCOME AB We study the effects of the property tax base shock caused by natural gas drilling in the Barnett Shale in Texasa state that taxes oil and gas wells as property. Over the boom and bust in drilling, housing appreciation closely followed the oil and gas property tax base, which expanded the total tax base by 23 percent at its height. The expansion led to a decline in property tax rates while maintaining or increasing revenues to schools. Overall, each $1 per student increase in the oil and gas property tax base increased the value of the typical home by $0.15. Some evidence suggests that the cumulative density of wells nearby may lower housing values, indicating that drilling could reduce local welfare without policies to increase local public revenues. C1 [Weber, Jeremy G.] Univ Pittsburgh, Grad Sch Publ & Int Affairs, 3601 Wesley W Posvar Hall, Pittsburgh, PA 15260 USA. [Burnett, J. Wesley] Coll Charleston, Dept Econ, Beatty Ctr, 5 Liberty St,Suite 413, Charleston, SC 29424 USA. [Xiarchos, Irene M.] USDA, Off Energy Policy & New Uses, 1400 Independence Ave SW, Washington, DC 20250 USA. [Xiarchos, Irene M.] USDA, Off Chief Economist, 1400 Independence Ave SW, Washington, DC 20250 USA. RP Weber, JG (reprint author), Univ Pittsburgh, Grad Sch Publ & Int Affairs, 3601 Wesley W Posvar Hall, Pittsburgh, PA 15260 USA. EM jgw99@pitt.edu; burnettjw@cofc.edu; ixiarchos@oce.usda.gov OI Burnett, James/0000-0001-9299-3481 FU Office of Energy Policy and New Uses (U.S. Department of Agriculture) [58-0111-12-009]; Oak Ridge Associated Universities FX We thank Camille Salama, Amanda Harker, Elaine Hill, Tara Mathews, Sara Melo de Weber, Max Harleman, editor Kenneth Couch, and four anonymous reviewers for their comments and assistance. The Office of Energy Policy and New Uses (U.S. Department of Agriculture, Cooperative Agreement 58-0111-12-009) and Oak Ridge Associated Universities provided financial support for this research. The views expressed are those of the authors and should not be attributed to the U.S. Department of Agriculture. All errors and opinions are our own. NR 68 TC 0 Z9 0 U1 2 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0276-8739 EI 1520-6688 J9 J POLICY ANAL MANAG JI J. Policy Anal. Manage. PD SUM PY 2016 VL 35 IS 3 BP 587 EP + DI 10.1002/pam.21911 PG 32 WC Economics; Public Administration SC Business & Economics; Public Administration GA DP6RZ UT WOS:000378627600005 ER PT J AU Fatima, T Sobolev, AP Teasdale, JR Kramer, M Bunce, J Handa, AK Mattoo, AK AF Fatima, Tahira Sobolev, Anatoly P. Teasdale, John R. Kramer, Matthew Bunce, Jim Handa, Avtar K. Mattoo, Autar K. TI Fruit metabolite networks in engineered and non-engineered tomato genotypes reveal fluidity in a hormone and agroecosystem specific manner SO METABOLOMICS LA English DT Article DE Agro-environment and metabolomics; Ethylene; Metabolite networks; Methyl jasmonate; Polyamines; S-adenosylmethionine decarboxylase ID LEGUME COVER CROP; GENE-EXPRESSION; ABIOTIC STRESS; GABA SHUNT; TRANSCRIPTOME; ACCUMULATION; POLYAMINES; ETHYLENE; PATHWAY; PLANTS AB Introduction Metabolomics provides a view of endogenous metabolic patterns not only during plant growth, development and senescence but also in response to genetic events, environment and disease. The effects of the field environment on plant hormone-specific metabolite profiles are largely unknown. Few studies have analyzed useful phenotypes generated by introducing single or multiple gene events alongside the non-engineered wild type control at field scale to determine the robustness of the genetic trait and its modulation in the metabolome as a function of specific agroecosystem environments. Objectives We evaluated the influence of genetic background (high polyamine lines; low methyl jasmonate line; low ethylene line; and isogenic genotypes carrying double transgenic events) and environments (hairy vetch, rye, plastic black mulch and bare soil mulching systems) on the metabolomic profile of isogenic reverse genetic mutations and selected mulch based cropping systems in tomato fruit. Net photosynthesis and fruit yield were also determined. Methods NMR spectroscopy was used for quantifying metabolites that are central to primary metabolism. We analyzed both the first moment (means) of metabolic response to genotypes and agroecosystems by traditional univariate/multivariate methods, and the second moment (covariances) of responses by creating networks that depicted changes in correlations of paired metabolites. This particular approach is novel and was necessary because our experimental material yielded highly variable metabolic responses that could not be easily understood using the traditional analytical approaches for first moment statistics. Results High endogenous spermidine and spermine content exhibited strong effects on amino acids, Krebs cycle intermediates and energy molecules (ADP + ATP) in ripening fruits of plants grown under different agroecosystem environments. The metabolic response to high polyamine genotypes was similar to the response to hairy vetch cover crop mulch; supported by the pattern of changes in correlation between metabolites. Changes in primary metabolites of genotypes mutated for the deficiency of ethylene or methyl jasmonate were unique under all growth conditions and opposite of high polyamine genotype results. The high polyamine trait was found to dominate the low ethylene and low jasmonate mutations under field conditions. For several metabolites low ethylene and low methyl jasmonate genotypes had an inverse relationship. Collectively, these results affirm that interactions between metabolite pathways and growth environments are affected by genotype, and influence the metabolite quality of a crop. Conclusion This study portrays how metabolite relationships change, both in mean and in correlation, under different genotypic and environmental conditions. Although these networks are surprisingly dynamic, we also find examples of selectively conserved associations. C1 [Fatima, Tahira; Teasdale, John R.; Mattoo, Autar K.] ARS, Sustainable Agr Syst Lab, USDA, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Sobolev, Anatoly P.] CNR, Magnet Resonance Lab Annalaura Segre, Inst Chem Methodol, Rome, Italy. [Kramer, Matthew] ARS, Stat Grp, USDA, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Bunce, Jim] ARS, Crop Syst Lab, USDA, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Handa, Avtar K.] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA. [Fatima, Tahira] Univ Western Ontario, London, ON, Canada. RP Mattoo, AK (reprint author), ARS, Sustainable Agr Syst Lab, USDA, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM autar.mattoo@ars.usda.gov NR 53 TC 3 Z9 3 U1 4 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1573-3882 EI 1573-3890 J9 METABOLOMICS JI Metabolomics PD JUN PY 2016 VL 12 IS 6 AR 103 DI 10.1007/s11306-016-1037-2 PG 15 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DP8MS UT WOS:000378752900008 ER PT J AU Seim, GL Tako, E Ahn, C Glahn, RP Young, SL AF Seim, Gretchen L. Tako, Elad Ahn, Cedric Glahn, Raymond P. Young, Sera L. TI A Novel in Vivo Model for Assessing the Impact of Geophagic Earth on Iron Status SO NUTRIENTS LA English DT Article DE geophagy; iron absorption; intestine; broiler chicken; anaemia; pica; clay ID VITRO DIGESTION/CACO-2 MODEL; POULTRY GALLUS-GALLUS; PREGNANT-WOMEN; BIOAVAILABLE IRON; ANEMIA; ABSORPTION; PICA; CLAY; NUTRITION; SOILS AB The causes and consequences of geophagy, the craving and consumption of earth, remain enigmatic, despite its recognition as a behavior with public health implications. Iron deficiency has been proposed as both a cause and consequence of geophagy, but methodological limitations have precluded a decisive investigation into this relationship. Here we present a novel in vivo model for assessing the impact of geophagic earth on iron status: Gallus gallus (broiler chicken). For four weeks, animals were gavaged daily with varying dosages of geophagic material or pure clay mineral. Differences in haemoglobin (Hb) across treatment groups were assessed weekly and differences in liver ferritin, liver iron, and gene expression of the iron transporters divalent metal transporter 1 (DMT1), duodenal cytochrome B (DcytB) and ferroportin were assessed at the end of the study. Minimal impact on iron status indicators was observed in all non-control groups, suggesting dosing of geophagic materials may need refining in future studies. However, this model shows clear advantages over prior methods used both in vitro and in humans, and represents an important step in explaining the public health impact of geophagy on iron status. C1 [Seim, Gretchen L.] Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA. [Tako, Elad; Glahn, Raymond P.] ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Ahn, Cedric] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA. [Young, Sera L.] Cornell Univ, Dept Populat Med, Ithaca, NY 14853 USA. [Young, Sera L.] Northwestern Univ, Dept Anthropol, Evanston, IL 60208 USA. RP Young, SL (reprint author), Cornell Univ, Dept Populat Med, Ithaca, NY 14853 USA.; Young, SL (reprint author), Northwestern Univ, Dept Anthropol, Evanston, IL 60208 USA. EM gs373@cornell.edu; et79@cornell.edu; cahn954@gmail.com; Raymond.Glahn@ars.usda.gov; sera.young@cornell.edu NR 48 TC 2 Z9 2 U1 4 U2 5 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-6643 J9 NUTRIENTS JI Nutrients PD JUN PY 2016 VL 8 IS 6 AR 362 DI 10.3390/nu8060362 PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DP8YF UT WOS:000378783200047 ER PT J AU Chervyakova, OV Zaitsev, VL Iskakov, BK Tailakova, ET Strochkov, VM Sultankulova, KT Sandybayev, NT Stanbekova, GE Beisenov, DK Abduraimov, YO Mambetaliyev, M Sansyzbay, AR Kovalskaya, NY Nemchinov, LG Hammond, RW AF Chervyakova, Olga V. Zaitsev, Valentin L. Iskakov, Bulat K. Tailakova, Elmira T. Strochkov, Vitaliy M. Sultankulova, Kulyaisan T. Sandybayev, Nurlan T. Stanbekova, Gulshan E. Beisenov, Daniyar K. Abduraimov, Yergali O. Mambetaliyev, Muratbay Sansyzbay, Abylay R. Kovalskaya, Natalia Y. Nemchinov, Lev. G. Hammond, Rosemarie W. TI Recombinant Sheep Pox Virus Proteins Elicit Neutralizing Antibodies SO VIRUSES-BASEL LA English DT Article DE sheep pox virus; vaccinia virus; recombinant protein; prokaryotic expression; neutralizing antibodies; immunogenicity ID INTRACELLULAR MATURE VIRIONS; VACCINIA VIRUS; ENVELOPE PROTEIN; EXTRACELLULAR VIRUS; MEMBRANE-PROTEINS; GOAT-POX; CAPRIPOXVIRUS INFECTIONS; TRANSMEMBRANE PROTEIN; MONOCLONAL-ANTIBODIES; NUCLEOTIDE-SEQUENCE AB The aim of this work was to evaluate the immunogenicity and neutralizing activity of sheep pox virus (SPPV; genus Capripoxvirus, family Poxviridae) structural proteins as candidate subunit vaccines to control sheep pox disease. SPPV structural proteins were identified by sequence homology with proteins of vaccinia virus (VACV) strain Copenhagen. Four SPPV proteins (SPPV-ORF 060, SPPV-ORF 095, SPPV-ORF 117, and SPPV-ORF 122), orthologs of immunodominant L1, A4, A27, and A33 VACV proteins, respectively, were produced in Escherichia coli. Western blot analysis revealed the antigenic and immunogenic properties of SPPV-060, SPPV-095, SPPV-117 and SPPV-122 proteins when injected with adjuvant into experimental rabbits. Virus-neutralizing activity against SPPV in lamb kidney cell culture was detected for polyclonal antisera raised to SPPV-060, SPPV-117, and SPPV-122 proteins. To our knowledge, this is the first report demonstrating the virus-neutralizing activities of antisera raised to SPPV-060, SPPV-117, and SPPV-122 proteins. C1 [Chervyakova, Olga V.; Zaitsev, Valentin L.; Tailakova, Elmira T.; Strochkov, Vitaliy M.; Sultankulova, Kulyaisan T.; Sandybayev, Nurlan T.; Abduraimov, Yergali O.; Mambetaliyev, Muratbay; Sansyzbay, Abylay R.] RK ME&S Sci Comm, Res Inst Biol Safety Problems, Gvardeiskiy 080409, Kazakhstan. [Iskakov, Bulat K.; Stanbekova, Gulshan E.; Beisenov, Daniyar K.] RK ME&S Sci Comm, MA Aitkhozhins Inst Mol Biol & Biochem, Alma Ata 050012, Kazakhstan. [Kovalskaya, Natalia Y.; Nemchinov, Lev. G.; Hammond, Rosemarie W.] ARS, USDA, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. RP Hammond, RW (reprint author), ARS, USDA, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. EM ovch@mail.ru; biosafety@biosafety.kz; bulat.isakakov@mail.ru; tailakova_86@mail.ru; vstrochkov@biosafety.kz; sultankul70@mail.ru; nurlan.s@mail.ru; gulshanst@yahoo.com; daniyar.b@mail.ru; yergali.a@gmail.com; murat@biosafety.kz; sansyzbai-ar@biosafety.kz; natalia.kovalksaya@ars.usda.gov; nemchinov@ars.usda.gov; rose.hammond@ars.usda.gov RI Strochkov, Vitaliy/B-6743-2012; Chervyakova, Olga/H-3095-2015 OI Strochkov, Vitaliy/0000-0003-3399-2942; Chervyakova, Olga/0000-0002-7954-6246 FU International project ISTC [K-1704] FX The research was conducted under the International project ISTC # K-1704 "Development of Methods to Construct Recombinant Prophylactic Means for Sheep Pox with use of Transgenic Plants." Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 70 TC 0 Z9 0 U1 3 U2 4 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4915 J9 VIRUSES-BASEL JI Viruses-Basel PD JUN PY 2016 VL 8 IS 6 AR 159 DI 10.3390/v8060159 PG 13 WC Virology SC Virology GA DP9WM UT WOS:000378848600011 ER PT J AU Adkins, JK Barton, CD Grubbs, S Stringer, JW Kolka, RK AF Adkins, Joshua K. Barton, Christopher D. Grubbs, Scott Stringer, Jeffrey W. Kolka, Randall K. TI Assessment of Streamside Management Zones for Conserving Benthic Macroinvertebrate Communities Following Timber Harvest in Eastern Kentucky Headwater Catchments SO WATER LA English DT Article DE forestry best management practices; aquatic macroinvertebrates; headwater streams; water quality ID RIPARIAN ZONES; BIOTIC INDEX; DISTURBANCE; ECOSYSTEM; USA; ASSEMBLAGES; WATERSHEDS; VEGETATION; POLLUTION; ECOLOGY AB Headwater streams generally comprise the majority of stream area in a watershed and can have a strong influence on downstream food webs. Our objective was to determine the effect of altering streamside management zone (SMZ) configurations on headwater aquatic insect communities. Timber harvests were implemented within six watersheds in eastern Kentucky. The SMZ configurations varied in width, canopy retention and best management practice (BMP) utilization at the watershed scale. Benthic macroinvertebrate samples collected one year before and four years after harvest indicated few differences among treatments, although post-treatment abundance was elevated in some of the treatment streams relative to the unharvested controls. Jaccard index values were similar across SMZ treatments after logging, indicating strong community overlap. These findings suggest that stream invertebrate communities did respond to the timber harvest, though not negatively. Results also suggest that SMZ criteria for aquatic habitats in steeply sloping topography, including at least 50 percent canopy retention and widths of at least 16.8 m, appear to be adequate for protecting benthic macroinvertebrate communities from logging impacts. C1 [Adkins, Joshua K.] Transylvania Univ, Dept Biol, 300 North Broadway, Lexington, KY 40508 USA. [Barton, Christopher D.; Stringer, Jeffrey W.] Univ Kentucky, Dept Forestry, 105 TP Cooper Bldg, Lexington, KY 40546 USA. [Grubbs, Scott] Western Kentucky Univ, Dept Biol, TCCW 334, Bowling Green, KY 42101 USA. [Kolka, Randall K.] USDA Forest Serv, Ctr Res Ecosyst Change, 1831 Hwy 169 East, Grand Rapids, MN 55744 USA. RP Barton, CD (reprint author), Univ Kentucky, Dept Forestry, 105 TP Cooper Bldg, Lexington, KY 40546 USA. EM jadkins@transy.edu; barton@uky.edu; scott.grubbs@wku.edu; jeffrey.stringer@uky.edu; rkolka@fs.fed.us FU Kentucky Agricultural Experiment Station FX This work was supported in part by the Kentucky Agricultural Experiment Station. A special thanks to Andrea Drayer for providing assistance in the field and laboratory. NR 69 TC 0 Z9 0 U1 18 U2 22 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2073-4441 J9 WATER-SUI JI Water PD JUN PY 2016 VL 8 IS 6 AR 261 DI 10.3390/w8060261 PG 15 WC Water Resources SC Water Resources GA DP9XN UT WOS:000378851300045 ER PT J AU Lanigan, D Stout, J Anderson, W AF Lanigan, David Stout, John Anderson, William TI Atmospheric stability and diurnal patterns of aeolian saltation on the Llano Estacado SO AEOLIAN RESEARCH LA English DT Article ID SOUTHERN HIGH-PLAINS; SURFACE-LAYER; BOUNDARY-LAYERS; NEW-MEXICO; SAND; TRANSPORT; MODELS; TEXAS; DUST; WIND AB Aeolian transport is driven by aerodynamic surface stress imposed by turbulent winds in the Earth's atmospheric boundary layer (ABL). ABL regime is influenced by stratification, which can either enhance or suppress production of turbulence by shear associated with the vertical gradient of streamwise velocity. During the day and night, surface heat fluxes induce a negative (unstable) and positive (stable) vertical gradient of potential temperature, respectively, which modifies the role of buoyancy in turbulence production. During the brief morning and evening transition periods, the vertical gradient of potential temperature vanishes (neutral stratification). The Monin-Obukhov similarity theory describes how the vertical gradient of streamwise velocity varies with stratification. Simultaneous field measurement of wind speed and aeolian activity were obtained over a 218-day period on a bare, sandy surface on the high plains of the Llano Estacado region of west Texas. Wind speed was measured at a height of 2 m with a propeller-type anemometer and aeolian activity was measured at the surface with a piezoelectric saltation sensor. We have used the wind speed measurements within the framework of the Monin-Obukhov similarity theory to estimate "typical" shear velocity, u(*), of the ABL as stratification is varied (characterized with the stability parameter). This approach results in a color flood contour of u(*) against time of day and stability parameter: the procedure demonstrates that aeolian activity is most likely to occur during the day, when buoyancy acts in conjunction with mechanical shear to increase u(*). (C) 2016 Elsevier B.V. All rights reserved. C1 [Lanigan, David; Anderson, William] Univ Texas Dallas, Dept Mech Engn, 800 W Campbell Rd, Richardson, TX 75080 USA. [Stout, John] ARS, USDA, 3810 4th St, Lubbock, TX 79415 USA. RP Anderson, W (reprint author), Univ Texas Dallas, Dept Mech Engn, 800 W Campbell Rd, Richardson, TX 75080 USA. EM wca140030@utdallas.edu FU National Science Foundation [AGS-1500224, AGS-1542855] FX This work was supported by the National Science Foundation (Program Manager: Drs. N. Anderson, C. Lu, and E. Bensman) under Award No. AGS-1500224 (and Summer 2015 REU Supplement Award No. AGS-1542855). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The U.S. Department of Agriculture is an equal opportunity provider and employer. NR 34 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1875-9637 EI 2212-1684 J9 AEOLIAN RES JI Aeolian Res. PD JUN PY 2016 VL 21 BP 131 EP 137 DI 10.1016/j.aeolia.2016.04.001 PG 7 WC Geography, Physical SC Physical Geography GA DP3YL UT WOS:000378432000012 ER PT J AU Valpiani, NH Wilde, PE Rogers, BL Stewart, HG AF Valpiani, Natalie H. Wilde, Parke E. Rogers, Beatrice L. Stewart, Hayden G. TI Price Differences across Farmers' Markets, Roadside Stands, and Supermarkets in North Carolina SO APPLIED ECONOMIC PERSPECTIVES AND POLICY LA English DT Article DE Fruit and vegetable marketing; food retail; local food; North Carolina ID VEGETABLE INTAKE; FRUIT; FOOD; CONSUMPTION AB Whether direct farmer-to-consumer outlets compete with supermarkets on produce prices remains an empirical question; marketing costs are not consistently higher in one retail channel or the other. This study compared prices of 29 fruits and vegetables across North Carolina farmers' markets, roadside stands, and supermarkets. Larger farmers' markets had higher prices: three fruits and one vegetable were cheaper at a direct outlet, while four vegetables were cheaper at supermarkets. Weighting item prices by consumption share attenuated differences in mean price across outlets. Direct-retail outlets are price competitive and should be considered among other tools to boost fresh fruit and vegetable intake. C1 [Valpiani, Natalie H.; Wilde, Parke E.; Rogers, Beatrice L.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Medford, MA 02155 USA. [Stewart, Hayden G.] USDA, Econ Res Serv, Washington, DC USA. RP Valpiani, NH (reprint author), Tufts Univ, Friedman Sch Nutr Sci & Policy, Medford, MA 02155 USA. EM Natalie.Valpiani@gmail.com FU National Science Foundation Graduate Research Fellowship [DGF-0806676] FX This material is based upon work supported by the National Science Foundation Graduate Research Fellowship under Grant No. DGF-0806676. NR 39 TC 1 Z9 1 U1 8 U2 10 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 2040-5790 EI 2040-5804 J9 APPL ECON PERSPECT P JI Appl. Econ. Perspect. Policy PD JUN PY 2016 VL 38 IS 2 BP 276 EP 291 DI 10.1093/aepp/ppv018 PG 16 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA DO9PL UT WOS:000378118800005 ER PT J AU Katchova, AL Ahearn, MC AF Katchova, Ani L. Ahearn, Mary Clare TI Dynamics of Farmland Ownership and Leasing: Implications for Young and Beginning Farmers SO APPLIED ECONOMIC PERSPECTIVES AND POLICY LA English DT Article DE Farmland ownership; farmland leasing; beginning farmers; young farmers AB This study considers the transition into farming and growth of new farmers in U.S. agriculture by examining land ownership and leasing trends. Our approach is to characterize the entire distribution by farmer age and farmer experience rather than using young versus old and beginning versus established farmer categories. We also use a linked-farms longitudinal approach to show trends over time in farmland expansion and contraction. We find that farms operated by older beginning farmers tend to be smaller and do not tend to grow over time. Our results show that it is mostly young farmers as opposed to all beginning farmers who rapidly expand their farm operations after entering agriculture. Our findings inform policy makers about the strategies that young and beginning farmers use to start their businesses and expand over time, and suggest more effective approaches for targeting loan programs to both young and beginning farmers. C1 [Katchova, Ani L.] Ohio State Univ, Dept Agr Environm & Dev Econ, Columbus, OH 43210 USA. [Ahearn, Mary Clare] USDA, Econ Res Serv, Washington, DC USA. EM katchova.1@osu.edu NR 31 TC 0 Z9 0 U1 4 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 2040-5790 EI 2040-5804 J9 APPL ECON PERSPECT P JI Appl. Econ. Perspect. Policy PD JUN PY 2016 VL 38 IS 2 BP 334 EP 350 DI 10.1093/aepp/ppv024 PG 17 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA DO9PL UT WOS:000378118800008 ER PT J AU Kapczynski, DR Dorsey, K Chrzastek, K Moraes, M Jackwood, M Hilt, D Gardin, Y AF Kapczynski, Darrell R. Dorsey, Kristi Chrzastek, Klaudia Moraes, Mauro Jackwood, Mark Hilt, Debra Gardin, Yannick TI Vaccine Protection of Turkeys Against H5N1 Highly Pathogenic Avian Influenza Virus with a Recombinant Turkey Herpesvirus Expressing the Hemagglutinin Gene of Avian Influenza SO AVIAN DISEASES LA English DT Article DE highly pathogenic avian influenza virus; HVT-AI vector vaccine; potency; H5N1; turkey ID DISEASE VIRUS; CHICKENS; EFFICACY; POULTRY; CHALLENGE; STRATEGIES; ANTIBODIES; AMERICA; BIRDS AB Outbreaks of H5 highly pathogenic avian influenza (HPAI) in commercial poultry are a constant threat to animal health and food supplies. While vaccination can enhance protection and reduce the spread of disease, there is considerable evidence that the level of immunity required for protection varies by subtype and virulence of field virus. In this study, the efficacy of a recombinant turkey herpesvirus (rHVT) vector vaccine expressing the hemagglutinin gene from a clade 2.2 AI virus (A/Swan/Hungary/4999/2006) was evaluated in turkeys for protection against challenge with A/Whooper Swan/Mongolia/L244/2005 H5N1 HPAI clade 2.2. One-day-old turkeys received a single vaccination and were challenged at 4 wk postvaccination with 2 x 3 10(6) 50% embryo infectious dose per bird. The results demonstrate that following H5N1 HPAI challenge 96% protection was observed in rHVT-AI vaccinated turkeys. The oral and cloacal swabs taken from challenged birds demonstrated that vaccinated birds had lower incidence and titers of viral shedding compared with sham-vaccinated birds. From respiratory and gastrointestinal tracts, there was a greater than 6 log(10) reduction in shedding in vaccinated birds as compared with the controls. This study provides support for the use of a commercially available rHVT-AI vaccine to protect turkeys against H5N1 HPAI. C1 [Kapczynski, Darrell R.; Chrzastek, Klaudia] ARS, Exot & Emerging Avian Dis Res Unit, US Natl Poultry Hlth Res Ctr, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. [Dorsey, Kristi; Moraes, Mauro] Ceva Anim Hlth, Lenexa, KS USA. [Jackwood, Mark; Hilt, Debra] Univ Georgia, Coll Vet Med, Dept Populat Hlth, Athens, GA USA. [Gardin, Yannick] Ceva Anim Hlth, Libourne, France. RP Kapczynski, DR (reprint author), ARS, Exot & Emerging Avian Dis Res Unit, US Natl Poultry Hlth Res Ctr, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. EM darrell.kapczynski@ars.usda.gov NR 35 TC 0 Z9 0 U1 6 U2 6 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2016 VL 60 IS 2 BP 413 EP 417 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA DO9EH UT WOS:000378086600002 PM 27309280 ER PT J AU Garber, L Bjork, K Patyk, K Rawdon, T Antognoli, M Delgado, A Ahola, S McCluskey, B AF Garber, Lindsey Bjork, Kathe Patyk, Kelly Rawdon, Thomas Antognoli, Maria Delgado, Amy Ahola, Sara McCluskey, Brian TI Factors Associated with Highly Pathogenic Avian Influenza H5N2 Infection on Table-Egg Layer Farms in the Midwestern United States, 2015 SO AVIAN DISEASES LA English DT Article DE avian influenza; H5N2; highly pathogenic; risk factors; table-egg layers ID MULTIPLE RISK-FACTORS; POULTRY; SPREAD; VIRUS AB A case-control study was conducted among commercial table-egg layer and pullet operations in Iowa and Nebraska, United States, to investigate potential risk factors for infection with highly pathogenic avian influenza (HPAI) H5N2. A questionnaire was developed and administered to 28 case farms and 31 control farms. Data were collected at the farm and barn levels, enabling two separate analyses to be performed-the first a farm-level comparison of case farms vs. control farms, and the second a barn-level comparison between case barns on case farms and control barns on control farms. Multivariable logistic regression models were fit using a forward-selection procedure. Key risk factors identified were farm location in an existing control zone, rendering and garbage trucks coming near barns, dead-bird disposal located near barns, and visits by a company service person. Variables associated with a decreased risk of infection included visitors changing clothing, cleaning and disinfecting a hardsurface barn entryway, and ceiling/eaves ventilation in barns. C1 [Garber, Lindsey; Bjork, Kathe; Patyk, Kelly; Antognoli, Maria; Delgado, Amy; Ahola, Sara; McCluskey, Brian] Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Sci Technol & Anal Serv,Ctr Epidemiol & Anim Hlth, 2150 Ctr Ave,Bldg B, Ft Collins, CO 80526 USA. [Rawdon, Thomas] Minist Primary Ind, Incurs Invest & Surveillance Team, Invest Diagnost Ctr, 66 Ward St, Upper Hutt 5040, New Zealand. [Rawdon, Thomas] Minist Primary Ind, Response Directorate, 66 Ward St, Upper Hutt 5040, New Zealand. RP Garber, L (reprint author), Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Sci Technol & Anal Serv,Ctr Epidemiol & Anim Hlth, 2150 Ctr Ave,Bldg B, Ft Collins, CO 80526 USA. EM Lindsey.P.Garber@aphis.usda.gov NR 18 TC 0 Z9 0 U1 6 U2 6 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2016 VL 60 IS 2 BP 460 EP 466 PG 7 WC Veterinary Sciences SC Veterinary Sciences GA DO9EH UT WOS:000378086600010 PM 27309288 ER PT J AU Dargatz, D Beam, A Wainwright, S McCluskey, B AF Dargatz, David Beam, Andrea Wainwright, Sherri McCluskey, Brian TI Case Series of Turkey Farms from the H5N2 Highly Pathogenic Avian Influenza Outbreak in the United States During 2015 SO AVIAN DISEASES LA English DT Article DE highly pathogenic avian influenza; H5N2; United States; outbreak; epidemiology ID VIRUS; AMERICA; SPREAD AB Between December 2014 and June 2015, an outbreak of H5N2 HPAI caused the largest and most expensive agriculture emergency in U.S. Department of Agriculture-Animal and Plant Health Inspection Service history. The outbreak affected 21 states; 232 poultry farms (211 commercial and 21 backyard) were affected, and approximately 49.6 million birds were depopulated on poultry farms. The majority of affected farms were commercial turkey operations (n = 160). This report is a case series describing 104 H5N2 HPAI-affected turkey farms in Iowa, Minnesota, Missouri, North Dakota, South Dakota, and Wisconsin that had H5N2 HPAI virus detected between March 5 and June 1, 2015. The farm manager or farm personnel voluntarily completed an epidemiologic questionnaire administered by state and federal animal health officials. Equipment and vehicle sharing with other farms was common, particularly for feed trucks (77% of farms shared feed trucks with other farms), live haul loaders (90.4%), poult trailers (72.0%), and preloaders (80.7%). Many farms had water bodies in proximity to the farm, such as a pond (42.6%) or stream (21.8%). About one-third of farms (33.7%) reported seeing wild birds inside the turkey barns. Only 44.2% of farms reported that third-party biosecurity audits or assessments had been conducted. Because the newly introduced Asian H5N8 HPAI and two new HPAI viruses, H5N2 and H5N1, are now circulating in U.S. wild birds, primarily migratory waterfowl, a greater potential for reoccurrence exists with the spring and fall migratory seasons, representing higher risk periods for outbreaks of HPAI in commercial poultry farms in the future. Eliminating exposure to wild birds, especially waterfowl or environments contaminated by wild waterfowl, will reduce risk of reintroduction of H5N2 HPAI virus, and ensuring good onfarm biosecurity will help the poultry industry avoid introduction of influenza and lateral spread between farms. C1 [Dargatz, David; Wainwright, Sherri; McCluskey, Brian] USDA, Anim & Plant Hlth Inspect Serv, Vet Serv, 2150 Ctr Ave,Bldg B, Ft Collins, CO 80526 USA. [Beam, Andrea] USDA, Anim & Plant Hlth Inspect Serv, Plant Protect & Quarantine, 13601 Old Cutler Rd,Bldg 63, Miami, FL 33158 USA. RP Dargatz, D (reprint author), USDA, Anim & Plant Hlth Inspect Serv, Vet Serv, 2150 Ctr Ave,Bldg B, Ft Collins, CO 80526 USA. EM david.a.dargatz@aphis.usda.gov NR 11 TC 1 Z9 1 U1 8 U2 10 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2016 VL 60 IS 2 BP 467 EP 472 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA DO9EH UT WOS:000378086600011 PM 27309289 ER PT J AU Lee, DH Kwon, JH Park, JK Yuk, SS Tseren-Ochir, EO Noh, JY Lee, JB Park, SY Choi, IS Song, CS AF Lee, Dong-Hun Kwon, Jung-Hoon Park, Jae-Keun Yuk, Seong-Su Tseren-Ochir, Erdene-Ochir Noh, Jin-Yong Lee, Joong-Bok Park, Seung-Yong Choi, In-Soo Song, Chang-Seon TI Experimental Infection of Chickens with Intercontinental Reassortant H9N2 Influenza Viruses from Wild Birds SO AVIAN DISEASES LA English DT Article DE avian influenza virus; wild bird; chicken; H9N2 ID A VIRUSES; HONG-KONG; POULTRY; TURKEYS; CHINA; KOREA; ASIA AB The H9N2 subtype of low pathogenic avian influenza (LPAI) virus is the most prevalent LPAI in domestic poultry. We previously reported the natural reassortant H9N2 viruses between North American and Eurasian lineages isolated from wild birds in Korea. These viruses were identified in China and Alaska, providing evidence of intercontinental dispersal. In this study, we evaluated the infectivity, transmissibility, and pathogenic potential of these H9N2 viruses and Eurasian H9N2 virus identified from wild birds using specific-pathogen-free chickens. Three-week-old chickens were infected intranasally. All of these reassortant H9N2 viruses could not be replicated and transmitted in chickens. On the other hand, three out of eight chickens inoculated with the Eurasian H9N2 virus shed detectable levels of virus and showed seroconversion but did not show contact transmission of the virus. Although all reassortant H9N2 viruses could not be replicated and transmitted in chickens, and although there are no reports on reassortant H9N2 virus infection in poultry farms until now, monitoring of reassortant H9N2 viruses should be continued to prepare for the advent and evolution of these viruses. C1 [Lee, Dong-Hun; Kwon, Jung-Hoon; Park, Jae-Keun; Yuk, Seong-Su; Tseren-Ochir, Erdene-Ochir; Noh, Jin-Yong; Lee, Joong-Bok; Park, Seung-Yong; Choi, In-Soo; Song, Chang-Seon] Konkuk Univ, Coll Vet Med, Avian Dis Lab, 1 Hwayang Dong, Seoul 143701, South Korea. [Lee, Dong-Hun] USDA, SE Poultry Res Lab, 934 Coll Stn Rd, Athens, GA 30605 USA. RP Song, CS (reprint author), Konkuk Univ, Coll Vet Med, Avian Dis Lab, 1 Hwayang Dong, Seoul 143701, South Korea. EM songcs@konkuk.ac.kr FU Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries, Republic of Korea [313016032HD020] FX This work was supported by grants from the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (No. 313016032HD020), Republic of Korea. NR 18 TC 0 Z9 1 U1 4 U2 5 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2016 VL 60 IS 2 BP 493 EP 495 PG 3 WC Veterinary Sciences SC Veterinary Sciences GA DO9EH UT WOS:000378086600015 PM 27309293 ER PT J AU Day, JM Zsak, L AF Day, J. Michael Zsak, Laszlo TI Molecular Characterization of Enteric Picornaviruses in Archived Turkey and Chicken Samples from the United States SO AVIAN DISEASES LA English DT Article DE turkey; chicken; enteric; picornavirus; phylogeny ID MULTIPLE SEQUENCE ALIGNMENT; NEIGHBOR-JOINING METHOD; FAMILY PICORNAVIRIDAE; METAGENOMIC ANALYSIS; WEIGHT-GAIN; VIRUSES; GENOME; POULTS; IDENTIFICATION; HEPATITIS AB Recent metagenomic analyses of the enteric viromes in turkeys and chickens have revealed complex viral communities comprised of multiple viral families. Of particular significance are the novel avian picobirnaviruses (family Picobirnaviridae), multiple genera of tailed phages (family Siphoviridae), and undescribed avian enteric picornaviruses (family Picornaviridae). In addition to these largely undescribed-and therefore relatively poorly understood-poultry enteric viral families, these metagenomic analyses have also revealed the presence of well-known groups of enteric viruses such as the chicken and turkey astroviruses (family Astroviridae) and the avian rotaviruses and reoviruses (family Reoviridae). The order Picornavirales is a group of viruses in flux, particularly among the avian picornaviruses, since several new genera have been described recently based upon community analysis of enteric viromes from poultry and other avian species worldwide. Our previous investigation of the turkey enteric picornaviruses suggests the avian enteric picornaviruses may contribute to the enteric disease syndromes and performance problems often observed in turkeys in the Southeastern United States. This report describes our recent phylogenetic analysis of turkey and chicken enteric samples archived at the Southeast Poultry Research Laboratory from 2004 to present and is a first step in placing these novel avian picornaviruses within the larger Picornaviridae family. C1 [Day, J. Michael; Zsak, Laszlo] USDA ARS, US Natl Poultry Res Ctr, SE Poultry Res Lab, 934 Coll Stn Rd, Athens, GA 30605 USA. RP Day, JM (reprint author), USDA ARS, US Natl Poultry Res Ctr, SE Poultry Res Lab, 934 Coll Stn Rd, Athens, GA 30605 USA. EM michael.day@ars.usda.gov FU USDA/ARS Intervention Strategies to Control and Prevent Enteric Viral Diseases of Poultry [CRIS 6612-32000-067-00D]; United States Poultry and Egg Association (USPEA) FX Laura Ferguson is acknowledged for excellent technical support. Thanks to industry stakeholders for field samples and for their continued support. This research was supported by USDA/ARS CRIS 6612-32000-067-00D Intervention Strategies to Control and Prevent Enteric Viral Diseases of Poultry and by a grant from the United States Poultry and Egg Association (USPEA). NR 44 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2016 VL 60 IS 2 BP 500 EP 505 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA DO9EH UT WOS:000378086600017 PM 27309295 ER PT J AU Zeilinger, AR Olson, DM Andow, DA AF Zeilinger, Adam R. Olson, Dawn M. Andow, David A. TI Competitive release and outbreaks of non-target pests associated with transgenic Bt cotton SO ECOLOGICAL APPLICATIONS LA English DT Article DE Bt cotton; Gossypium hirsutum; induced resistance; non-target effects; Pentatomidae; pest outbreak; transgenic insecticidal crops ID GREEN STINK BUG; LEPIDOPTERA-NOCTUIDAE; PHYTOPHAGOUS INSECTS; NILAPARVATA-LUGENS; INDUCED RESISTANCE; BROWN PLANTHOPPER; SECONDARY PESTS; NEZARA-VIRIDULA; HERBIVORE; PLANT AB The adoption of transgenic Bt cotton has, in some cases, led to environmental and economic benefits through reduced insecticide use. However, the distribution of these benefits and associated risks among cotton growers and cotton-growing regions has been uneven due in part to outbreaks of non-target or secondary pests, thereby requiring the continued use of synthetic insecticides. In the southeastern USA, Bt cotton adoption has resulted in increased abundance of and damage from stink bug pests, Euschistus servus and Nezara viridula (Heteroptera: Pentatomidae). While the impact of increased stink bug abundance has been well-documented, the causes have remained unclear. We hypothesize that release from competition with Bt-susceptible target pests may drive stink bug outbreaks in Bt cotton. We first examined the evidence for competitive release of stink bugs through meta-analysis of previous studies. We then experimentally tested if herbivory by Bt-susceptible Helicoverpa zea increases stink bug leaving rates and deters oviposition on non-Bt cotton. Consistent with previous studies, we found differences in leaving rates only for E. servus, but we found that both species strongly avoided ovipositing on H. zea-damaged plants. Considering all available evidence, competitive release of stink bug populations in Bt cotton likely contributes to outbreaks, though the relative importance of competitive release remains an open question. Ecological risk assessments of Bt crops and other transgenic insecticidal crops would benefit from greater understanding of the ecological mechanisms underlying non-target pest outbreaks and greater attention to indirect ecological effects more broadly. C1 [Zeilinger, Adam R.] Univ Minnesota, Dept Entomol, Conservat Biol Program, St Paul, MN 55108 USA. [Olson, Dawn M.] ARS, Crop Protect Res & Management Unit, USDA, Tifton, GA 31794 USA. [Andow, David A.] Univ Minnesota, Dept Entomol, St Paul, MN 55108 USA. [Andow, David A.] Univ Minnesota, Ctr Community Genet, St Paul, MN 55108 USA. [Zeilinger, Adam R.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, 130 Mulford Hall, Berkeley, CA 94720 USA. RP Zeilinger, AR (reprint author), Univ Minnesota, Dept Entomol, Conservat Biol Program, St Paul, MN 55108 USA.; Zeilinger, AR (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, 130 Mulford Hall, Berkeley, CA 94720 USA. EM arz@berkeley.edu FU National Research Initiative of the US Department of Agriculture, National Institute of Food and Agriculture [2008-35302-04709]; USDA Regional Research Project [NC-205]; US National Science Foundation IGERT program [DGE-0653827]; Dayton-Wilkie Fund of the Bell Museum of Natural History, University of Minnesota FX This work was supported by the National Research Initiative of the US Department of Agriculture, National Institute of Food and Agriculture (grant number 2008-35302-04709 to D. A. Andow and D. M. Olson); the USDA Regional Research Project NC-205 (to D. A. Andow); the US National Science Foundation IGERT program (grant number DGE-0653827 to the University of Minnesota); and a thesis research grant, a doctoral dissertation fellowship, and grants from the Dayton-Wilkie Fund of the Bell Museum of Natural History, University of Minnesota (to A. R. Zeilinger). We thank Andy Hornbuckle, Tania Brown, and Jada Ransom for assistance in performing the experiments; Michael Toews for providing insects; and George Heimpel, Matt Daugherty, and two anonymous reviewers for helpful comments on early drafts of this paper. NR 64 TC 0 Z9 0 U1 16 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD JUN PY 2016 VL 26 IS 4 BP 1047 EP 1054 DI 10.1890/15-1314 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DO9GK UT WOS:000378092900007 PM 27509747 ER PT J AU Elkasabi, Y Chagas, BME Mullen, CA Boateng, AA AF Elkasabi, Yaseen Chagas, Bruna M. E. Mullen, Charles A. Boateng, Akwasi A. TI Hydrocarbons from Spirulina Pyrolysis Bio-oil Using One-Step Hydrotreating and Aqueous Extraction of Heteroatom Compounds SO ENERGY & FUELS LA English DT Article; Proceedings Paper CT 22nd International Symposium on Combustion Processes (ISoCP-2015) CY SEP 22-25, 2015 CL Polish Jurassic Highland, POLAND ID GAS REACTIVE PYROLYSIS; PARTHENIUM-ARGENTATUM PYROLYSIS; BIODIESEL PRODUCTION; PROTEINACEOUS BIOMASS; CATALYTIC PYROLYSIS; MICROALGAE; CHEMICALS; DISTILLATION; BIOREFINERY; BIOFUELS AB Biomass feedstocks such as algae and cyanobacteria are highly sought after because of Their high reproduction rates and growth densities, but their high concentrations of O and N heteroatoms are problematic for biofuel applications. Mild upgrading processes are necessary for producing fungible fuels from these renewable sources. We developed a process to upgrade spirulina-based bio-oil from tail-gas reactive pyrolysis (TGRP), using a combination of catalytic hydrotreatment and purification of the upgraded product.. The TGRP bio-oil was distillated at high organic yields, and the,distillates served-as the feedstock for catalytic upgrading. Simultaneous hydrodeoxygenation and hydrodenitrogenation (HDO/HDN) was carried out in one step using a commercial ruthenium catalyst on carbon support. Using bio-oil distillates as feedstocks for hydrotreatment produced hydrocarbons at high space velocities: Reactor temperature was the critical variable, wherein the-optimal temperature compromised between excessive yields loss and catalyst inactivity: While the HDO/HDN product contained relatively significant amounts of residual O and N (similar to 1 wt % each), the remaining O and N-containing compounds were removed via single aqueous phase extraction with hydrochloric acid. The extraction step serves as a milder alternative to deep HDO/HDN processes that diminish final product yields. C1 [Elkasabi, Yaseen; Mullen, Charles A.; Boateng, Akwasi A.] ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Chagas, Bruna M. E.] Univ Fed Rio Grande do Norte, Dept Chem Engn, BR-59078970 Natal, RN, Brazil. RP Elkasabi, Y (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM yaseen.elkasabi@ars.usda.gov FU USDA-NIFA-BRDI [2012-10008-20271] FX We thank Craig Einfeldt for operation and maintenance of the pyrolysis experiments, Dr. Gary Strahan for NMR experiments, and Protick Khoshnabish and Jennifer Gallup (co-op students, Drexel University) for conducting the distillation and characterization experiments. We also thank Johnson Matthey (UK) for providing the catalyst used in this study. A USDA-NIFA-BRDI Grant 2012-10008-20271 is hereby acknowledged. NR 31 TC 0 Z9 0 U1 11 U2 11 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 EI 1520-5029 J9 ENERG FUEL JI Energy Fuels PD JUN PY 2016 VL 30 IS 6 BP 4925 EP 4932 DI 10.1021/acs.energyfuels.6b00473 PG 8 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA DP0QZ UT WOS:000378195600056 ER PT J AU Sicard, P Augustaitis, A Belyazid, S Calfapietra, C de Marco, A Fenn, M Bytnerowicz, A Grulke, N He, S Matyssek, R Serengil, Y Wieser, G Paoletti, E AF Sicard, Pierre Augustaitis, Algirdas Belyazid, Salim Calfapietra, Carlo de Marco, Alessandra Fenn, Mark Bytnerowicz, Andrzej Grulke, Nancy He, Shang Matyssek, Rainer Serengil, Yusuf Wieser, Gerhard Paoletti, Elena TI Global topics and novel approaches in the study of air pollution, climate change and forest ecosystems SO ENVIRONMENTAL POLLUTION LA English DT Article; Proceedings Paper CT 27th Biennial Conference on Global Challenges of Air Pollution and Climate Change to Forests CY JUN 01-05, 2015 CL Nice, FRANCE DE Ozone; Nitrogen deposition; Climatic stressors; Interactions; Urban forests ID VOLATILE ORGANIC-COMPOUNDS; LEAF-AREA INDEX; SOUTHERN EUROPEAN FORESTS; OZONE RISK-ASSESSMENT; GROUND-LEVEL OZONE; NITROGEN DEPOSITION; UNITED-STATES; ISOPRENE EMISSION; FAGUS-SYLVATICA; CRITICAL LOADS AB Research directions from the 27th conference for Specialists in Air Pollution and Climate Change Effects on Forest Ecosystems (2015) reflect knowledge advancements about (i) Mechanistic bases of tree responses to multiple climate and pollution stressors, in particular the interaction of ozone (O-3) with nitrogen (N) deposition and drought; (ii) Linking genetic control with physiological whole-tree activity; (iii) Epigenetic responses to climate change and air pollution; (iv) Embedding individual tree performance into the multi-factorial stand-level interaction network; (v) Interactions of biogenic and anthropogenic volatile compounds (molecular, functional and ecological bases); (vi) Estimating the potential for carbon/pollution mitigation and cost effectiveness of urban and periurban forests; (vii) Selection of trees adapted to the urban environment; (viii) Trophic, competitive and host/parasite relationships under changing pollution and climate; (ix) Atmosphere -iosphere-pedosphere interactions as affected by anthropospheric changes; (x) Statistical analyses for epidemiological investigations; (xi) Use of monitoring for the validation of models; (xii) Holistic view for linking the climate, carbon, N and O-3 odelling; (xiii) Inclusion of multiple environmental stresses (biotic and abiotic) in critical load determinations; (xiv) Ecological impacts of N deposition in the under-investigated areas; (xv) Empirical models for mechanistic effects at the local scale; (xvi) Broad-scale N and sulphur deposition input and their effects on forest ecosystem services; (xvii) Measurements of dry deposition of N; (xviii) Assessment of evapotranspiration; (xix) Remote sensing assessment of hydrological parameters; and (xx) Forest management for maximizing water provision and overall forest ecosystem services. Ground-level O-3 is still the phytotoxic air pollutant of major concern to forest health. Specific issues about O-3 are: (xxi) Developing dose response relationships and stomatal O-3 flux parameterizations for risk assessment, especially, in under-investigated regions; (xxii) Defining biologically based O-3 standards for protection thresholds and critical levels; (xxiii) Use of free-air exposure facilities; (xxiv) Assessing O-3 impacts on forest ecosystem services. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Sicard, Pierre] ACRI HE, 260 Route Pin Montard BP234, F-06904 Sophia Antipolis, France. [Augustaitis, Algirdas] Aleksandras Stulginskis Univ, Studentu 13, LT-53362 Kaunas Dstr, Lithuania. [Belyazid, Salim] Univ Stockholm, S-10691 Stockholm, Sweden. [Calfapietra, Carlo] CNR, IBAF, Via Marconi 2, Porano, Italy. [de Marco, Alessandra] ENEA, Rome, Italy. [Fenn, Mark; Bytnerowicz, Andrzej] US Forest Serv, USDA, PSW Res Stn, 4955 Canyon Crest Dr, Riverside, CA 92507 USA. [Grulke, Nancy] WWETAC Prineville, Prineville, OR USA. [He, Shang] Chinese Acad Forestry, Inst Forest Ecol Environm & Protect, Beijing, Peoples R China. [Matyssek, Rainer] Tech Univ Munich, Ecophysiol Plants, Carlowitz Pl 2, D-85354 Freising Weihenstephan, Germany. [Serengil, Yusuf] Istanbul Univ, Istanbul, Turkey. [Wieser, Gerhard] Fed Res & Training Ctr Forests Nat Hazards & Land, Div Alpine Timberline Ecophysiol, Rennweg 1, A-6020 Innsbruck, Austria. [Paoletti, Elena] CNR, IPSP, Via Madonna Del Piano 10, I-50019 Sesto Fiorentino, Italy. RP Paoletti, E (reprint author), CNR, IPSP, Via Madonna Del Piano 10, I-50019 Sesto Fiorentino, Italy. EM Pierre.Sicard@acri-he.fr; Algirdas.Augustaitis@asu.lt; salim@belyazid.com; carlo.calfapietra@ibaf.cnr.it; alessandra.demarco@enea.it; mfenn@fs.fed.us; abytnerowicz@fs.fed.us; ngrulke@fs.fed.us; shanghechina@126.com; matyssek@wzw.tum.de; serengil@istanbul.edu.tr; gerhard.wieser@uibk.ac.at; elena.paoletti@cnr.it RI Paoletti, Elena/B-8974-2009; OI Paoletti, Elena/0000-0001-5324-7769; De Marco, Alessandra/0000-0001-7200-2257 FU General Council "Alpes-Maritimes"; City of Nice (Comite Doyen Jean Lepine); Regional Council "Provence-Alpes-Cote-d'Azur" (DDSC-SECA) FX We wish to thank all the speakers and participants, for contributing their knowledge and participating to the discussion. The conference organization was made possible with the financial support of the General Council "Alpes-Maritimes", the City of Nice (Comite Doyen Jean Lepine) and the Regional Council "Provence-Alpes-Cote-d'Azur" (DDSC-SECA). NR 155 TC 4 Z9 4 U1 34 U2 68 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 EI 1873-6424 J9 ENVIRON POLLUT JI Environ. Pollut. PD JUN PY 2016 VL 213 BP 977 EP 987 DI 10.1016/j.envpol.2016.01.075 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA DO6VO UT WOS:000377921800104 PM 26873061 ER PT J AU Johnson, S Bearer, S Hille, A Stout, S Turcotte, R AF Johnson, Sarah Bearer, Scott Hille, Andrea Stout, Susan Turcotte, Rick TI Eastern Hemlock Conservation: A Collaborative Approach to Prioritization through a Diverse Partnership SO ENVIRONMENTAL PRACTICE LA English DT Review ID WOOLLY ADELGID HEMIPTERA; EMERALD ASH BORER; NORTH-AMERICA; ALLEGHENY PLATEAU; FOREST RESPONSE; UNITED-STATES; PENNSYLVANIA; INFESTATION; DYNAMICS; SURVIVAL AB Eastern hemlock [Tsuga canadensis (L.) Carriere] is a valuable component of Allegheny Plateau forests in northwestern Pennsylvania and western New York. Since the 1950s, hemlock forests throughout the Central Appalachians have been under threat from a nonnative forest insect pest, the hemlock woolly adelgid (Adelges tsugae Annand). In 2012, to address this threat at the most meaningful scale, the United States Forest Service and The Nature Conservancy organized a diverse partnership to develop a strategy for landscape-level conservation of hemlock on the High Allegheny Unglaciated Plateau. The main goal of the partnership was to locate hemlock across the landscape regardless of land ownership and prioritize the hemlock for monitoring and protection from the adelgid. The priority Hemlock Conservation Areas that were identified by this partnership provide a guide for focusing limited financial and personnel resources, with the goal of protecting at least a portion of these areas from the impacts of the adelgid until more long-term management techniques are identified. To protect the important hemlock forests identified in this prioritization, a partnership of private and public land managers are forming a Cooperative Pest Management Area to continue this important collaboration, allocate scarce resources across the area, and allow private partners access to public funding for protection of priority hemlock on their lands. C1 [Johnson, Sarah; Bearer, Scott] Nature Conservancy, 2101 North Front St,Bldg 1,Suite 200, Harrisburg, PA 17110 USA. [Hille, Andrea] US Forest Serv, Allegheny Natl Forest, Warren, PA USA. [Stout, Susan] US Forest Serv, No Res Stn, Irvine, PA USA. [Turcotte, Rick] US Forest Serv, State & Private Forestry, Morgantown, WV USA. RP Johnson, S (reprint author), Nature Conservancy, 2101 North Front St,Bldg 1,Suite 200, Harrisburg, PA 17110 USA. EM sejohnson@tnc.org NR 41 TC 0 Z9 0 U1 16 U2 18 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1466-0466 EI 1466-0474 J9 ENVIRON PRAC JI Environ. Pract. PD JUN PY 2016 VL 18 IS 2 BP 94 EP 105 DI 10.1017/S1466046616000107 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA DP1EN UT WOS:000378233400004 ER PT J AU Tillman, PG AF Tillman, P. Glynn TI Diversity of stink bug (Hemiptera: Pentatomidae) egg parasitoids in woodland and crop habitats in southwest Georgia, USA SO FLORIDA ENTOMOLOGIST LA English DT Article DE Telenomus podisi; Trissolcus edessae; Anastatus reduvii; Anastatus mirabils ID TRISSOLCUS-EUSCHISTI HYMENOPTERA; HETEROPTERA-PENTATOMIDAE; TELENOMUS-PODISI; NEZARA-VIRIDULA; ASHMEAD HYMENOPTERA; DEVELOPMENTAL RATE; COTTON FARMSCAPES; NATURAL ENEMIES; HOST PLANTS; WILD HOSTS AB Euschistus servus (Say), Chinavia hilaris (Say), and Nezara viridula (L.) (Hemiptera: Pentatomidae) are economic pests of row crops. They move within and between closely associated crop and non-crop habitats throughout the growing season in response to deteriorating suitability of their current host plants. This study was conducted to investigate parasitism of naturally occurring C. hilaris and E. servus eggs in woodland habitats and crops alongside these habitats in southwest Georgia, USA. Also, parasitism of sentinel eggs of N. viridula was examined in a woodland habitat. Ten species of parasitoids, including 7 scelionids, 2 eupelmids, and 1 encyrtid, parasitized E. servus eggs. Telenomus podisi Ashmead (Hymenoptera: Scelionidae) was the most prevalent parasitoid of E. servus eggs in each of 3 habitats: woodlands, an early-season crop, and late-season crops. In woodlands, 27.9% of E. servus eggs were parasitized by Anastatus reduvii (Howard) and A. mirabilis (Walsh & Riley) (Hymenoptera: Eupelmidae). Four species of parasitoids, including 1 scelionid, 2 eupelmids, and 1 encyrtid, parasitized C. hilaris eggs. Trissolcus edessae Fouts (Hymenoptera: Scelionidae) was the most prevalent parasitoid of C. hilaris eggs in woodlands and the only parasitoid of C. hilaris eggs in late-season crops. In woodlands, 40.7% of C. hilaris eggs were parasitized by A. reduvii and A. mirabilis. In a woodland habitat, 6.6% of N. viridula sentinel eggs were parasitized by A. reduvii females, Anastatus males, and 1 encyrtid. Anastatus species were the only parasitoids that existed primarily in woodland habitats. In conclusion, a diversity of parasitoid species parasitizes native stink bug eggs in southwest Georgia, and species of parasitoids emerging from stink bug eggs can vary by habitat. C1 [Tillman, P. Glynn] ARS, USDA, Crop Protect & Management Res Lab, POB 748, Tifton, GA 31793 USA. RP Tillman, PG (reprint author), ARS, USDA, Crop Protect & Management Res Lab, POB 748, Tifton, GA 31793 USA. EM Glynn.Tillman@ars.usda.gov NR 48 TC 2 Z9 2 U1 3 U2 4 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2016 VL 99 IS 2 BP 286 EP 291 PG 6 WC Entomology SC Entomology GA DP0FI UT WOS:000378163800020 ER PT J AU Tillman, G Gaskin, J Endale, D Johnson, C Schomberg, H AF Tillman, Glynn Gaskin, Julia Endale, Dinku Johnson, Carroll Schomberg, Harry TI Parasitism of Megacopta cribraria (Hemiptera: Plataspidae) by Paratelenomus saccharalis (Hymenoptera: Platygastridae) in organic soybean plots in Georgia, USA SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material DE kudzu bug; conventional tillage; no-till ID RANGE AB Megacopta cribraria (F.) (Hemiptera: Plataspidae) is a newly invasive, exotic pest of soybean (Glycine max [L.] Merr.; Fabales: Fabaceae) in the southeastern United States. In 2013, the exotic egg parasitoid Paratelenomus saccharalis (Dodd) (Hymenoptera: Platygastridae) was discovered parasitizing eggs of this pest in kudzu (Pueraria montana var. lobata [Willd.] Maesen & S. Almeida; Fabales: Fabaceae) and soybean in 3 states in this region of the United States. We evaluated parasitism of M. cribraria egg masses by P. saccharalis in conventional tillage and no-till organic soybean experimental plots in 2013. Density of M. cribraria egg masses was significantly higher in conventional tillage soybean than in no-till soybean in weeks 2 through 5 for the 7 wk period M. cribraria egg masses were detected on soybean. Percentage of parasitism of M. cribraria egg masses by P. saccharalis was significantly higher in conventional tillage soybean (58.4%) than in no-till soybean (44.9%). In general, parasitism rates of egg masses were higher in conventional tillage soybean, where M. cribraria egg mass density was higher, than in no-till soybean. C1 [Tillman, Glynn; Johnson, Carroll] ARS, USDA, Crop Protect & Management Res Lab, POB 748, Tifton, GA 31793 USA. [Gaskin, Julia] Univ Georgia, Crop & Soil Sci, 4115B Miller Plant Sci Bldg, Athens, GA 30602 USA. [Endale, Dinku] ARS, USDA, Southeast Watershed Res Lab, POB 748, Tifton, GA 31793 USA. [Schomberg, Harry] ARS, USDA, Sustainable Agr Syst Lab, 10300 Baltimore Ave,Bldg 001 Barc West, Beltsville, MD 20705 USA. RP Tillman, G (reprint author), ARS, USDA, Crop Protect & Management Res Lab, POB 748, Tifton, GA 31793 USA. EM Glynn.Tillman@ars.usda.gov NR 11 TC 0 Z9 0 U1 8 U2 8 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2016 VL 99 IS 2 BP 300 EP 302 PG 3 WC Entomology SC Entomology GA DP0FI UT WOS:000378163800023 ER PT J AU Pregmon, EA Lujo, S Norton, K Hartman, E Rohde, B Zagvazdina, N Mankin, RW AF Pregmon, Emily A. Lujo, Sylvia Norton, Kayla Hartman, Ethan Rohde, B. Zagvazdina, Nina Mankin, R. W. TI A "walker" tool to place Diaphorina citri (Hemiptera: Liviidae) adults at predetermined sites for bioassays of behavior in citrus (Sapindales: Rutaceae) trees SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material DE phototaxis; jumping plant lice; handling disturbance ID PSYLLIDAE; AGE AB A "walker" tool was developed to assist successful placement of Diaphorina citri Kuwayama (Hemiptera: Liviidae) adults at predetermined sites on citrus (Sapindales: Rutaceae) trees in behavioral bioassays. Use of the walker resulted in a significantly higher proportion of successful placements and a significantly lower number of jumps away from the predetermined site compared with the use of a brush, although on average it required an additional 108 s more than the 41 s required with the brush to complete a successful placement. Females and males performed similarly with both devices. Potentially, the walker is a useful placement tool under conditions where it is time and labor intensive to provide large numbers of insects with specific physiological characteristics for testing, e.g., when it is difficult to supply large numbers of D. citri adults of known sex, mating status, and age. Because phototaxis is commonly observed in psyllids, the walker may be of use also for bioassays with other economic pests such as Bactericera cockerelli (Sulc) (Hemiptera: Triozidae). C1 [Pregmon, Emily A.; Lujo, Sylvia; Norton, Kayla; Hartman, Ethan; Zagvazdina, Nina; Mankin, R. W.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Rohde, B.] Univ Florida, Dept Elect & Comp Engn, Gainesville, FL 32611 USA. RP Mankin, RW (reprint author), ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. EM richard.mankin@ars.usda.gov NR 15 TC 1 Z9 1 U1 3 U2 5 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2016 VL 99 IS 2 BP 308 EP 310 PG 3 WC Entomology SC Entomology GA DP0FI UT WOS:000378163800026 ER PT J AU Shelly, TE Kurashima, RS AF Shelly, Todd E. Kurashima, Rick S. TI Mixing male lures results in an effective multispecies bait for trapping Bactrocera (Diptera: Tephritidae) fruit flies SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material DE trapping; detection; semiochemical; invasive species ID METHYL EUGENOL; TRAPS; HAWAII AB Detection of Bactrocera Macquart (Diptera: Tephritidae) fruit flies relies on male-specific lures, methyl eugenol and cue lure, which are presented singly in traps. We compared the efficacy of the standard single-lure treatments against mixtures of methyl eugenol and raspberry ketone (a natural analogue of cue lure) deployed in the same trap. No significant differences were found in captures of Bactrocera cucurbitae (Coquillett) or B. dorsalis (Hendel) male flies over 8 wk intervals between traps baited with a single lure and traps baited with any of the 3 mixtures tested. C1 [Shelly, Todd E.; Kurashima, Rick S.] Anim & Plant Hlth Inspect Serv, USDA, 41-650 Ahiki St, Waimanalo, HI 96795 USA. RP Shelly, TE (reprint author), Anim & Plant Hlth Inspect Serv, USDA, 41-650 Ahiki St, Waimanalo, HI 96795 USA. EM todd.e.shelly@aphis.usda.gov NR 9 TC 0 Z9 0 U1 12 U2 14 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2016 VL 99 IS 2 BP 318 EP 320 PG 3 WC Entomology SC Entomology GA DP0FI UT WOS:000378163800029 ER PT J AU Kanga, LHB Marshall, K Legaspi, JC AF Kanga, Lambert H. B. Marshall, Keith Legaspi, Jesusa C. TI Mechanisms of insecticide resistance in field populations of the varroa mite (Acari: Mesostigmata: Varroidae) in Florida SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material DE honey bee; miticide; synergist; mixed-function oxidase; altered target site ID LEPIDOPTERA; NOCTUIDAE; JACOBSONI; ESTERASE AB The varroa mite (Acari: Mesostigmata: Varroidae) has developed resistance to the 2 major miticides (fluvalinate and coumaphos) registered for control of this invasive pest of bees (Hymenoptera: Apidae) in the United States. Comparative studies on miticide toxicity with and without the synergists piperonyl butoxide and formamidine indicated that enhanced metabolism by mixed-function oxidases and altered target site were the major mechanisms of resistance to organophosphorus and pyrethroid insecticides in varroa mite populations of northern Florida. C1 [Kanga, Lambert H. B.; Marshall, Keith; Legaspi, Jesusa C.] Florida A&M Univ, Ctr Biol Control, 6383 Mahan Dr, Tallahassee, FL 32307 USA. [Legaspi, Jesusa C.] ARS, USDA, CMAVE, 6383 Mahan Dr, Tallahassee, FL 32308 USA. RP Kanga, LHB (reprint author), Florida A&M Univ, Ctr Biol Control, 6383 Mahan Dr, Tallahassee, FL 32307 USA. EM lambert.kanga@famu.edu NR 18 TC 0 Z9 0 U1 9 U2 14 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2016 VL 99 IS 2 BP 324 EP 326 PG 3 WC Entomology SC Entomology GA DP0FI UT WOS:000378163800031 ER PT J AU Fischer, AP Spies, TA Steelman, TA Moseley, C Johnson, BR Bailey, JD Ager, AA Bourgeron, P Charnley, S Collins, BM Kline, JD Leahy, JE Littell, JS Millington, JDA Nielsen-Pincus, M Olsen, CS Paveglio, TB Roos, CI Steen-Adams, MM Stevens, FR Vukomanovic, J White, EM Bowman, DMJS AF Fischer, A. Paige Spies, Thomas A. Steelman, Toddi A. Moseley, Cassandra Johnson, Bart R. Bailey, John D. Ager, Alan A. Bourgeron, Patrick Charnley, Susan Collins, Brandon M. Kline, Jeffrey D. Leahy, Jessica E. Littell, Jeremy S. Millington, James D. A. Nielsen-Pincus, Max Olsen, Christine S. Paveglio, Travis B. Roos, Christopher I. Steen-Adams, Michelle M. Stevens, Forrest R. Vukomanovic, Jelena White, Eric M. Bowman, David M. J. S. TI Wildfire risk as a socioecological pathology SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Article ID WESTERN UNITED-STATES; DECISION-MAKING; FIRE MANAGEMENT; BOREAL FOREST; MEGA-FIRES; LANDSCAPE; CONTEXT; SYSTEMS; POLICY AB Wildfire risk in temperate forests has become a nearly intractable problem that can be characterized as a socioecological "pathology": that is, a set of complex and problematic interactions among social and ecological systems across multiple spatial and temporal scales. Assessments of wildfire risk could benefit from recognizing and accounting for these interactions in terms of socioecological systems, also known as coupled natural and human systems (CNHS). We characterize the primary social and ecological dimensions of the wildfire risk pathology, paying particular attention to the governance system around wildfire risk, and suggest strategies to mitigate the pathology through innovative planning approaches, analytical tools, and policies. We caution that even with a clear understanding of the problem and possible solutions, the system by which human actors govern fire-prone forests may evolve incrementally in imperfect ways and can be expected to resist change even as we learn better ways to manage CNHS. C1 [Fischer, A. Paige] Univ Michigan, Ann Arbor, MI 48109 USA. [Spies, Thomas A.; Kline, Jeffrey D.] US Dept Agr USDA Forest Serv, Corvallis, OR USA. [Steelman, Toddi A.] Univ Saskatchewan, Saskatoon, SK, Canada. [Moseley, Cassandra; Johnson, Bart R.] Univ Oregon, Eugene, OR 97403 USA. [Bailey, John D.; Olsen, Christine S.] Oregon State Univ, Corvallis, OR 97331 USA. [Ager, Alan A.] USDA Forest Serv, Pendleton, OR USA. [Bourgeron, Patrick; Vukomanovic, Jelena] Univ Colorado, Boulder, CO 80309 USA. [Charnley, Susan] USDA Forest Serv, Portland, OR USA. [Collins, Brandon M.] USDA Forest Serv, Davis, CA USA. [Leahy, Jessica E.] Univ Maine, Orono, ME USA. [Littell, Jeremy S.] US Geol Survey, Anchorage, AK USA. [Millington, James D. A.] Kings Coll London, London WC2R 2LS, England. [Nielsen-Pincus, Max] Portland State Univ, Portland, OR 97207 USA. [Paveglio, Travis B.] Univ Idaho, Moscow, ID 83843 USA. [Roos, Christopher I.] So Methodist Univ, Dallas, TX 75275 USA. [Steen-Adams, Michelle M.] Univ New England, Biddeford, ME USA. [Stevens, Forrest R.] Univ Louisville, Louisville, KY 40292 USA. [White, Eric M.] USDA Forest Serv, Olympia, WA USA. [Bowman, David M. J. S.] Univ Tasmania, Hobart, Tas, Australia. RP Fischer, AP (reprint author), Univ Michigan, Ann Arbor, MI 48109 USA. EM apfisch@umich.edu RI Millington, James/B-5931-2008; Bowman, David/A-2930-2011 OI Millington, James/0000-0002-5099-0001; Bowman, David/0000-0001-8075-124X FU US National Science Foundation's (NSF's) Coupled Human and Natural Systems Program (NSF) [CNH-1013296]; USDA Forest Service PNW Research Station; Joint Fire Science Program (JFSP) [12-5-01-15]; NSF [CNH-1013296, CNH-0816475, CNH-1313688, GEO-1114898, DEB-1414041] FX This paper emerged from a workshop sponsored by the US National Science Foundation's (NSF's) Coupled Human and Natural Systems Program (NSF grant CNH-1013296), the USDA Forest Service PNW Research Station, and the Joint Fire Science Program (JFSP Project Number 12-5-01-15). We acknowledge support from NSF grants CNH-1013296, CNH-0816475, CNH-1313688, GEO-1114898, and DEB-1414041, and we thank A Agrawal and DL Peterson for providing comments on an earlier version of this paper. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the US Government. NR 48 TC 2 Z9 2 U1 11 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1540-9295 EI 1540-9309 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD JUN PY 2016 VL 14 IS 5 BP 277 EP 285 DI 10.1002/fee.1283 PG 9 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DP1TL UT WOS:000378272200018 ER PT J AU Darwish, O Li, SX Matthews, B Alkharouf, N AF Darwish, Omar Li, Shuxian Matthews, Benjamin Alkharouf, Nadim TI Genome-wide functional annotation of Phomopsis longicolla isolate MSPL 10-6 SO GENOMICS DATA LA English DT Article DE Phomopsis longicolla; Phomopsis seed decay; Soybean pathogen; Functional annotation AB Phomopsis seed decay of soybean is caused primarily by the seed-borne fungal pathogen Phomopsis longicolla (syn. Diaporthe longicolla). This disease severely decreases soybean seed quality, reduces seedling vigor and stand establishment, and suppresses yield. It is one of the most economically important soybean diseases. In this study we annotated the entire genome of P. longicolla isolate MSPL 10-6, which was isolated from field-grown soybean seed in Mississippi, USA. This study represents the first reported genome-wide functional annotation of a seed borne fungal pathogen in the Diaporthe-Phomopsis complex. The P. longicolla genome annotation will enable research into the genetic basis of fungal infection of soybean seed and provide information for the study of soybean-fungal interactions. The genome annotation will also be a valuable resource for the research and agricultural communities. It will aid in the development of new control strategies for this pathogen. The annotations can be found from: http://bioinformatics.towson.edu/phomopsis_longicolla/download.html. NCBI accession number is: AYRD00000000. Published by Elsevier Inc. C1 [Darwish, Omar; Alkharouf, Nadim] Towson Univ, Dept Comp & Informat Sci, Towson, MD 21252 USA. [Li, Shuxian] USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. [Matthews, Benjamin] USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 21075 USA. RP Alkharouf, N (reprint author), Towson Univ, Dept Comp & Informat Sci, Towson, MD 21252 USA.; Li, SX (reprint author), USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. EM shuxian.li@ars.usda.gov; nalkharouf@towson.edu NR 9 TC 0 Z9 0 U1 3 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2213-5960 J9 GENOM DATA JI Genom. Data PD JUN PY 2016 VL 8 BP 67 EP 69 DI 10.1016/j.gdata.2016.03.006 PG 3 WC Genetics & Heredity SC Genetics & Heredity GA DO4OF UT WOS:000377761900014 PM 27222801 ER PT J AU Hausmann, JC Mans, C Gosling, A Miller, JL Chamberlin, T Dunn, JR Miller, PE Sladky, KK AF Hausmann, Jennifer C. Mans, Christoph Gosling, Allyson Miller, Jaimie L. Chamberlin, Tamara Dunn, John R. Miller, Paul E. Sladky, Kurt K. TI Bilateral Uveitis and Hyphema in a Catalina Macaw (Ara ararauna X Ara macao) With Multicentric Lymphoma SO JOURNAL OF AVIAN MEDICINE AND SURGERY LA English DT Article DE multicentric T-cell lymphoma; uveitis; corticosteroid; avian; Catalina macaw; Ara ararauna x Ara macao ID POLYMERASE-CHAIN-REACTION; RETICULOENDOTHELIOSIS VIRUS; MAREKS-DISEASE; LYMPHOSARCOMA; NEOPLASIA; PARROT; BIRDS AB A 20-year-old, female Catalina macaw (Ara ararauna 3 Ara macao) was presented with bilateral uveitis and hyphema. The hyphema initially improved with 0.12% prednisolone acetate ophthalmic drops (1 drop OU q4h for 7 days), but the hyphema recurred after the drops were tapered. The bird subsequently developed inappetance, weight loss, regurgitation, and lethargy and was euthanatized 24 days after initial presentation. Necropsy revealed marked splenomegaly and hepatomegaly, with significant mucosal ulcerations of the proventriculus and petechiation associated with both kidneys. Histopathologic examination revealed multicentric lymphoma, with neoplastic cells observed in ocular, splenic, hepatic, renal, proventricular, intestinal, pancreatic, and choanal tissue. Neoplastic lymphocytes effaced the iris, ciliary body, and the choroid of the eyes, and neoplastic lymphocytes were attached to the corneal endothelium and infiltrated the sclera, episclera, and conjunctivae. Immunohistochemical results indicated that the neoplastic lymphocytes were CD3(+) and CD79a(-), which is consistent with T-cell lymphoma. C1 [Hausmann, Jennifer C.; Mans, Christoph; Gosling, Allyson; Miller, Paul E.; Sladky, Kurt K.] Univ Wisconsin, Sch Vet Med, Dept Surg Sci, 2015 Linden Dr W, Madison, WI 53706 USA. [Miller, Jaimie L.; Chamberlin, Tamara] Univ Wisconsin, Sch Vet Med, Dept Pathobiol Sci, 2015 Linden Dr W, Madison, WI 53706 USA. [Dunn, John R.] ARS, Avian Dis & Oncol Lab, USDA, 4279 E Mt Hope Rd, E Lansing, MI 48823 USA. RP Hausmann, JC (reprint author), Univ Wisconsin, Sch Vet Med, Dept Surg Sci, 2015 Linden Dr W, Madison, WI 53706 USA. NR 24 TC 0 Z9 0 U1 79 U2 80 PU ASSOC AVIAN VETERINARIANS PI BOCA RATON PA PO BOX 811720, BOCA RATON, FL 33481 USA SN 1082-6742 EI 1938-2871 J9 J AVIAN MED SURG JI J. Avian Med. Surg. PD JUN PY 2016 VL 30 IS 2 BP 172 EP 178 PG 7 WC Veterinary Sciences SC Veterinary Sciences GA DO9BW UT WOS:000378080300011 PM 27315386 ER PT J AU Rinehart, JP Yocum, GD Kemp, WP Bowsher, JH AF Rinehart, Joseph P. Yocum, George D. Kemp, William P. Bowsher, Julia H. TI Optimizing Fluctuating Thermal Regime Storage of Developing Megachile rotundata (Hymenoptera: Megachilidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE cold storage; fluctuating thermal regime; alfalfa leafcutting bee; Megachile rotundata ID ALFALFA LEAFCUTTING BEE; HIGH-TEMPERATURE; SOLITARY BEE; ION HOMEOSTASIS; COLD INJURY; SURVIVAL; INCUBATION; EMERGENCE; COLEOPTERA; EXPOSURE AB The alfalfa leafcutting bee, Megachile rotundata (F.), is the primary pollinator for alfalfa seed production in North America. Under current management practice, developing pupae are incubated at 29-30 degrees C until the adults emerge for pollination. If unfavorable spring weather delays peak alfalfa bloom, managers will cool pupae to slow development, which can increase mortality and causes sublethal effects. Previously, we demonstrated that exposure to a fluctuating thermal regime (FTR) increases survival and extends the viable storage period. To determine the optimal conditions for FTR during storage of developing M. rotundata, we examined four variables: temperature of the daily warm pulse, duration of the warm pulse, number of weeks exposed to the FTR treatment, and developmental stage of the bee. Survival was measured by successful eclosion to the adult stage. Under all conditions, exposure to FTR increased survival compared with exposure to a constant 6 degrees C. When the temperature of the daily warm pulse was 20-25 degrees C from a base temperature of 6 degrees C, and the pulse duration was extended to 3 h, survival rates were as high as those observed under standard storage conditions (29 degrees C). Under this FTR storage protocol, bee managers can delay emergence for similar to 8 wk without significant decreases in survival. Our findings have substantial economic implications for bee management and alfalfa seed production by increasing the flexibility and efficiency of M. rotundata adult emergence. C1 [Rinehart, Joseph P.; Yocum, George D.; Kemp, William P.] USDA ARS, Red River Valley Agr Res Ctr, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58102 USA. [Bowsher, Julia H.] N Dakota State Univ, Dept Biol Sci, 201 Stevens Hall,POB 6050, Fargo, ND 58108 USA. RP Rinehart, JP (reprint author), USDA ARS, Red River Valley Agr Res Ctr, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58102 USA. EM Joseph.Rinehart@ars.usda.gov; george.yocum@ars.usda.gov; william.kemp@ars.usda.gov; julia.bowsher@ndsu.edu FU United States Department of Agriculture Agricultural Research Service FX We thank Carlee O'Dell and Marnie Larson for their technical assistance. This research was funded by the United States Department of Agriculture Agricultural Research Service. NR 32 TC 0 Z9 0 U1 4 U2 5 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2016 VL 109 IS 3 BP 993 EP 1000 DI 10.1093/jee/tow026 PG 8 WC Entomology SC Entomology GA DO8NX UT WOS:000378041400002 ER PT J AU Behle, RW Goett, EJ AF Behle, Robert W. Goett, Erica J. TI Dosage Response Mortality of Japanese Beetle, Masked Chafer, and June Beetle (Coleoptera: Scarabaeidae) Adults When Exposed to Experimental and Commercially Available Granules Containing Metarhizium brunneum SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE white grub; turf; biological control; Metarhizium brunneum; microsclerotia ID POPILLIA-JAPONICA COLEOPTERA; BEAUVERIA-BASSIANA; ANISOPLIAE; EFFICACY; MICROSCLEROTIA; GRUBS; PATHOGENICITY; LARVAE; FIELD AB Field-collected adults of three genera of turf-infesting scarabs, Japanese beetle (Popillia japonica Newman), June beetles (Phyllophaga spp.), and masked chafers (Cyclocephala spp.), were exposed to experimental and commercial granule formulations of the entomopathogenic fungus Metarhizium brunneum (Petch) strain F52 to determine their relative susceptibility. Experimental granules contained microsclerotia produced by liquid fermentation with the ability to produce fresh conidia when rehydrated and commercial granules were Met 52 granular bioinsecticide. All three groups of scarab adults showed a positive dosage response to the fungus when exposed in cups of potting mix treated with the granules. LC50 values for microsclerotia granules were 1.9 x 10(7), 7.1 x 10(6), and 3.2 x 10(6) conidia cup(-1) for P. japonica, Phyllophaga spp., and Cyclocephala spp., respectively. LC50 values for Met 52 granules were 5.9 x 10(7), 5.1 x 10(7), and 7.6 x 10(6) conidia cup(-1), respectively. The experimental granules containing microsclerotia show promise as a viable commercial control agent. They can be produced using lower cost fermentation methods and applied at lower dosages (97 g for 100 m(2) as opposed to 489 g per 100 m(2) for Met 52). If M. brunneum is applied to control the aforementioned white grubs, our data indicate the potential for the adult beetles to also be infected as they enter the soil to lay eggs. C1 [Behle, Robert W.; Goett, Erica J.] USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ Ave, Peoria, IL 61604 USA. RP Behle, RW (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ Ave, Peoria, IL 61604 USA. EM robert.behle@ars.usda.gov; erica.goett@ars.usda.gov FU United States Golf Association (USGA) [2012-12-446] FX This research was funded in part by the United States Golf Association (USGA ID number 2012-12-446) titled: "Biological Control of White Grubs Infesting Turf by the Application of Metarhizium anisopliae." NR 21 TC 0 Z9 0 U1 8 U2 11 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2016 VL 109 IS 3 BP 1109 EP 1115 DI 10.1093/jee/tow080 PG 7 WC Entomology SC Entomology GA DO8NX UT WOS:000378041400018 ER PT J AU Hanula, JL Mayfield, AE Reid, LS Horn, S AF Hanula, James L. Mayfield, Albert E., III Reid, Laurie S. Horn, Scott TI Influence of Trap Distance From a Source Population and Multiple Traps on Captures and Attack Densities of the Redbay Ambrosia Beetle (Coleoptera: Curculionidae: Scolytinae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE laurel wilt; Xyleborus glabratus; trap efficiency; effective trapping distance; cubeb oil ID LAUREL WILT DISEASE; SOUTHEASTERN UNITED-STATES; IPS-TYPOGRAPHUS L; EFFECTIVE ATTRACTION RADIUS; XYLEBORUS-GLABRATUS; RAFFAELEA-LAURICOLA; 1ST REPORT; MANUKA OIL; PHEROMONE TRAPS; CUBEB OIL AB The redbay ambrosia beetle, Xyleborus glabratus Eichhoff, is the principal vector of laurel wilt disease in North America. Lures incorporating essential oils of manuka plants (Leptospermum scoparium J. R. Forster & G. Forster) or cubeb seeds (Piper cubeba L.f.) are the most effective in-flight attractants to date. Using grids of traps baited with these essential oil lures, we evaluated 1) the effect of trap distance from a source beetle population on beetle captures, 2) the feasibility of trapping out low-density beetle populations, and 3) the effect of trap spacing on beetle captures. In the first experiment, increasing trap distance up to 300 m from a source X. glabratus population had little effect on beetle captures. In a second experiment conducted in a study area with very low beetle densities, trapping for 5 mo prior to deploying freshly cut, uninfested redbay bolts had no effect on subsequent attack densities. In a third experiment, numbers of X. glabratus captured in traps in the center of a grid of nine traps spaced 1 or 5 m apart were compared with lone baited or unbaited traps 30 m away. Relative to the more distant traps, the grid of baited traps neither increased captures in the unbaited center trap nor decreased captures in the baited center trap, regardless of spacing. The results suggest that the effective trapping distance of essential oil lures for X. glabratus is < 1 m, and that newer, more attractive lures will be needed to be useful in managing X. glabratus populations. C1 [Hanula, James L.; Horn, Scott] Forest Serv, USDA, Southern Res Stn, 320 Green St, Athens, GA 30602 USA. [Mayfield, Albert E., III] Forest Serv, USDA, Southern Res Stn, 200 WT Weaver Blvd, Asheville, NC 28804 USA. [Reid, Laurie S.] City Charlotte, 600 East 4th St, Charlotte, NC 28202 USA. RP Mayfield, AE (reprint author), Forest Serv, USDA, Southern Res Stn, 200 WT Weaver Blvd, Asheville, NC 28804 USA. EM jhanula@fs.fed.us; amayfield02@fs.fed.us; ldukes@ci.charlotte.nc.us; shorn01@fs.fed.us FU USDA Forest Service, Southern Research Station FX We thank Dr. Cavell Brownie for statistical advice, the South Carolina Department of Parks, Recreation, and Tourism for permission to work on Hunting Island State Park, and the Georgia Department of Natural Resources for permission to work on Ohoopee Dunes Natural Area. Andrew Tait and Mike Cody are thanked for assistance with field work. Funding for this work was through the USDA Forest Service, Southern Research Station. Mention of specific products does not imply endorsement by the USDA Forest Service. NR 52 TC 1 Z9 1 U1 3 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2016 VL 109 IS 3 BP 1196 EP 1204 DI 10.1093/jee/tow068 PG 9 WC Entomology SC Entomology GA DO8NX UT WOS:000378041400028 ER PT J AU Miller, DR Allison, JD Crowe, CM Dickinson, DM Eglitis, A Hofstetter, RW Munson, AS Poland, TM Reid, LS Steed, BE Sweeney, JD AF Miller, D. R. Allison, J. D. Crowe, C. M. Dickinson, D. M. Eglitis, A. Hofstetter, R. W. Munson, A. S. Poland, T. M. Reid, L. S. Steed, B. E. Sweeney, J. D. TI Pine Sawyers (Coleoptera: Cerambycidae) Attracted to alpha-Pinene, Monochamol, and Ipsenol in North America SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE alpha-pinene; monochamol; ipsenol; detection; trapping ID BARK BEETLE PHEROMONES; HOST-PLANT VOLATILES; AGGREGATION PHEROMONE; UNITED-STATES; TITILLATOR COLEOPTERA; WOOD NEMATODE; SCUTELLATUS; WOODBORERS; GALLOPROVINCIALIS; IDENTIFICATION AB Detection tools are needed for Monochamus species (Coleoptera: Cerambycidae) because they are known to introduce pine wilt disease by vectoring nematodes in Asia, Europe, and North America. In 2012-2014, we examined the effects of the semiochemicals monochamol and ipsenol on the flight responses of the sawyer beetles Monochamus carolinensis (Olivier), Monochamus clamator (LeConte), Monochamus mutator LeConte, Monochamus notatus (Drury), Monochamus obtusus Casey, Monochamus scutellatus (Say), and Monochamus titillator (F.) complex (Coleoptera: Cerambycidae) to traps baited with alpha-pinene. Experiments were set in pine forests in New Brunswick and Ontario (Canada), and Arizona, Georgia, Michigan, Montana, Oregon, South Carolina, Utah, and Washington (United States). In brief, 40 traps were placed in 10 blocks of 4 traps per block per location. Traps were baited with: 1) alpha-pinene; 2) alpha-pinene. monochamol; 3) alpha-pinene + ipsenol; and 4) alpha-pinene + monochamol + ipsenol. Monochamol increased catches of six species and one species complex of Monochamus with an additive effect of ipsenol for five species and one species complex. There was no evidence of synergy between monochamol and ipsenol on beetle catches. Monochamol had no effect on catches of other Cerambycidae or on any associated species of bark beetles, weevils, or bark beetle predators. We present a robust data set suggesting that the combination of alpha-pinene, ipsenol, and monochamol may be a useful lure for detecting Monochamus species. C1 [Miller, D. R.; Crowe, C. M.] USDA Forest Serv, Southern Res Stn, 320 Green St, Athens, GA 30602 USA. [Allison, J. D.] Nat Resources Canada, Great Lakes Forestry Ctr, 1219 Queen St East, Sault Ste Marie, ON P6A 2E5, Canada. [Dickinson, D. M.] USDA Forest Serv, Forest Hlth Protect, 1133 North Western Ave, Wenatchee, WA 98801 USA. [Eglitis, A.] USDA Forest Serv, Forest Hlth Protect, 63095 Deschutes Market Rd, Bend, OR 97701 USA. [Hofstetter, R. W.] No Arizona Univ, Sch Forestry, 200 East Pine Knoll Dr, Flagstaff, AZ 86011 USA. [Munson, A. S.] USDA Forest Serv, Forest Hlth Protect, 4746 South 1900 East, Ogden, UT 84403 USA. [Poland, T. M.] USDA Forest Serv, No Res Stn, 3101 Technol Blvd,Suite F, Lansing, MI 48910 USA. [Reid, L. S.] South Carolina Forestry Commiss, 5500 Broad River Rd, Columbia, SC 29212 USA. [Reid, L. S.] City Charlotte, 600 East Fourth St, Charlotte, NC 28202 USA. [Steed, B. E.] USDA Forest Serv, Forest Hlth Protect, 200 East Broadway,POB 7669, Missoula, MT 59807 USA. [Sweeney, J. D.] Nat Resources Canada, Canadian Forest Serv, Atlantic Forestry Ctr, 1350 Regent St,POB 4000, Fredericton, NB E3B 5P7, Canada. RP Miller, DR (reprint author), USDA Forest Serv, Southern Res Stn, 320 Green St, Athens, GA 30602 USA. EM dmiller03@fs.fed.us; jeremy.allison@canada.ca; ccrowe01@fs.fed.us; darcimdickinson@fs.fed.us; bugfellow15@gmail.com; rich.hofstetter@nau.edu; smunson@fs.fed.us; tpoland@fs.fed.us; ldukes@ci.charlotte.nc.us; bsteed@fs.fed.us; jon.sweeney@canada.ca NR 29 TC 0 Z9 0 U1 7 U2 7 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2016 VL 109 IS 3 BP 1205 EP 1214 DI 10.1093/jee/tow071 PG 10 WC Entomology SC Entomology GA DO8NX UT WOS:000378041400029 ER PT J AU Allison, JD Graham, EE Poland, TM Strom, BL AF Allison, Jeremy D. Graham, Elizabeth E. Poland, Therese M. Strom, Brian L. TI Dilution of Fluon Before Trap Surface Treatment Has No Effect on Longhorned Beetle (Coleoptera: Cerambycidae) Captures SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Cerambycidae; Monochamus; trap; survey and detection; Fluon ID FLIGHT INTERCEPT TRAPS; TETROPIUM-CASTANEUM L.; MULTIPLE-FUNNEL; PANEL TRAPS; SCUTELLATUS COLEOPTERA; AGGREGATION PHEROMONE; MULTIFUNNEL TRAPS; FUSCUM F; BUPRESTIDAE; EFFICIENCY AB Several studies have observed that trap captures of longhorned beetles (Coleoptera: Cerambycidae) can be increased by treating the surface of intercept traps with a lubricant. In addition to being expensive, these treatments can alter the spectral properties of intercept traps when applied neat. These surface treatments, particularly Fluon, are commonly used diluted as a low friction coating to prevent insects from climbing out of rearing containers. The purpose of this study was to examine the effect of diluting Fluon on the spectral properties of treated corrugated plastic traps and the capture of longhorned beetles including Monochamus scutellatus (Say), Monochamus mutator (LeConte), and Monochamus notatus (Drury). Intercept panel traps were baited with attractant semiochemicals and treated with either undiluted (i.e., 100%) Fluon, a 1: 1 mixture of Fluon and water (50%), a 1: 9 mixture of Fluon and water (10%), or untreated. There were no obvious differences in the relative reflectance of untreated black Coroplast plastic or black Coroplast plastic treated with 50 or 10% Fluon. Traps treated with 100% Fluon had similar patterns of peak reflectance to the other treatments but overall had higher relative reflectance. In general, no effect of diluting the Fluon was observed for male or female M. scutellatus or M. mutator, but an effect of treating traps with Fluon was observed. Similar results were observed for the combined captures of Clytus ruricola Olivier, Cyrtophorus verrucosus Olivier, Megacyllene caryae (Gahan), Xylotrechus colonus (F.), Neoclytus acuminatus (F.), Neoclytus mucronatus (F.), and Phymatodes testaceus (L.). No treatment effect was observed for M. notatus. C1 [Allison, Jeremy D.] Nat Resources Canada Canadian Forest Serv, Great Lakes Forestry Ctr, 1219 Queen St East, Sault Ste Marie, ON P6A 2E5, Canada. [Graham, Elizabeth E.; Poland, Therese M.] USDA FS, No Res Stn, 3101 Technol Blvd,Ste F, Lansing, MI 48910 USA. [Graham, Elizabeth E.] Michigan State Univ, Dept Entomol, 288 Farm Lane, E Lansing, MI 48824 USA. [Graham, Elizabeth E.] USDA FS, FHP, 11175 Auke Lake Way, Juneau, AK 99801 USA. [Strom, Brian L.] USDA FS, Southern Res Stn, 2500 Shreveport Highway, Pineville, LA 71360 USA. RP Allison, JD (reprint author), Nat Resources Canada Canadian Forest Serv, Great Lakes Forestry Ctr, 1219 Queen St East, Sault Ste Marie, ON P6A 2E5, Canada. EM Jeremy.Allison@NRCan.gc.ca; eegraham@fs.fed.us; tpoland@fs.fed.us; brianstrom@fs.fed.us FU Natural Resources Canada; USDA Forest Service Special Technology Development Program FX We thank Nick Boyonoski, Reg Nott, Chanel Fall, Katelyn Kotska, Martin Patton, Shannon Escasa, Sarah Crispell, Tom Baweja, and Tina Ciaramitaro for field and laboratory assistance; Norm Iles (Eacom Timber Corp.) for access to field sites, and Natural Resources Canada and USDA Forest Service Special Technology Development Program for funding. NR 44 TC 3 Z9 3 U1 3 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2016 VL 109 IS 3 BP 1215 EP 1219 DI 10.1093/jee/tow081 PG 5 WC Entomology SC Entomology GA DO8NX UT WOS:000378041400030 ER PT J AU Liu, YB Haynes, KF AF Liu, Yong-Biao Haynes, Kenneth F. TI Effects of Ultralow Oxygen and Vacuum Treatments on Bed Bug (Heteroptera: Cimicidae) Survival SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE ultralow oxygen; vacuum; bed bug control; pest; insect ID CIMEX-LECTULARIUS HEMIPTERA; MODIFIED ATMOSPHERES; TEMPERATURE; FUMIGATION; RESISTANCE; MORTALITY; PRESSURE; INSECTS; LETTUCE; LARVAE AB Control of bed bugs is problematic, balancing among efficacy, safety, and cost. In this study, ultralow oxygen (ULO) and vacuum treatments were tested on bed bugs to develop a safer, effective, and environmentally friendly solution to kill bed bugs on infested items. ULO treatments were established by flushing sealed enclosures with nitrogen. All life stages of bed bugs were found to be susceptible to ULO and vacuum treatments, and efficacy of the treatments increased with reduced oxygen levels, increased treatment time, and temperature. In the ULO treatments, 100% mortality of bed bug nymphs and adults and >98% mortality of bed bug eggs were achieved in the 8-h treatment under 0.1% O-2 atmosphere at 30 degrees C. Different levels of vacuum that yielded different oxygen levels were tested on all life stages of bed bugs. The susceptibility of different stages to vacuum treatments increased from nymphs to adults to eggs. Complete control of all life stages was achieved in 12 h under -982 mbar (-29.0 inHg) vacuum at 30 degrees C. This study demonstrated that bed bugs were very susceptible to low oxygen stresses and ULO and vacuum treatments have potential to be used as effective and safe treatments to decontaminate bed bug-infested removable objects. C1 [Liu, Yong-Biao] USDA ARS, 1636 E Alisal St, Salinas, CA 93905 USA. [Haynes, Kenneth F.] Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA. RP Liu, YB (reprint author), USDA ARS, 1636 E Alisal St, Salinas, CA 93905 USA. EM yongbiao.liu@ars.usda.gov; khaynes@email.uky.edu FU National Institute of Food and Agriculture, U.S. Department of Agriculture, Hatch Project [KY008066 1003549] FX Scott Bessin and Tiffany Masuda provided technical support. The work completed at the University of Kentucky (Kentucky Agricultural Experiment Station) was supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, Hatch Project KY008066 1003549. NR 32 TC 0 Z9 0 U1 10 U2 14 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2016 VL 109 IS 3 BP 1310 EP 1316 DI 10.1093/jee/tow034 PG 7 WC Entomology SC Entomology GA DO8NX UT WOS:000378041400043 ER PT J AU Zukoff, SN Ostlie, KR Potter, B Meihls, LN Zukoff, AL French, L Ellersieck, MR French, BW Hibbard, BE AF Zukoff, Sarah N. Ostlie, Kenneth R. Potter, Bruce Meihls, Lisa N. Zukoff, Anthony L. French, Lee Ellersieck, Mark R. French, B. Wade Hibbard, Bruce E. TI Multiple Assays Indicate Varying Levels of Cross Resistance in Cry3Bb1-Selected Field Populations of the Western Corn Rootworm to mCry3A, eCry3.1Ab, and Cry34/35Ab1 SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Bacillus thuringiensis; Diabrotica virgifera virgifera; insect resistance management; cross resistance; DAS-59122-7 ID COLEOPTERA-CHRYSOMELIDAE; TRANSGENIC MAIZE; BT-CORN; EVENT DAS-59122-7; MANAGEMENT; MORTALITY; SURVIVAL; SELECTION; IMPACT; LARVAE AB Minnesota populations of Diabrotica virgifera virgifera LeConte, the western corn rootworm, surviving Cry3Bb1-expressing corn in the field and western corn rootworm populations assumed to be susceptible to all Bt proteins were evaluated for susceptibility to Cry3Bb1, mCry3A, eCry3.1Ab, and Cry34/35Ab1 in diet assays and three different plant-based assays. Rootworm populations originating from Cry3Bb1 fields and that consistently experienced greater than expected damage had increased survival and larval growth compared to control populations assayed on Cry3Bb1 as well as mCry3a and eCry3.1Ab. Cross resistance was documented between Cry3Bb1 and both mCry3A and eCry3.1Ab as single toxins. Despite very high resistance ratios in some comparisons, cross resistance was not complete and also varied with the population being evaluated, the trait measured, and the susceptible rootworm population used for comparison. Regardless of resistance and cross resistance, all proteins, even Cry3Bb1, retained some efficacy in terms of either reducing rootworm larval growth, protecting plants from damage, or both, for all rootworm populations evaluated. For one Cry3Bb1-selected population, a resistance ratio of 9.1-fold was found to Cry34/35Ab1 when evaluating EC50 values relative to a susceptible control population; however, resistance to Cry34/35Ab1 was not evident in all assays in this population. The United States Environmental Protection Agency recently suggested eliminating diet assays as part of the Bt resistance monitoring process. However, given the variability of responses of western corn rootworm populations to different proteins in different assays, both plant and diet assays are needed as options for detecting and fully characterizing resistance. C1 [Zukoff, Sarah N.; Zukoff, Anthony L.] Univ Missouri, Div Plant Sci, Columbia, MO USA. [Zukoff, Sarah N.] Kansas State Univ, Southwest Res & Extens Ctr, Garden City, NY USA. [Ostlie, Kenneth R.] Univ Minnesota, Dept Entomol, St Paul, MN 55108 USA. [Potter, Bruce] Univ Minnesota, Southwest Res & Outreach Ctr, Lamberton, MN USA. [Meihls, Lisa N.; Hibbard, Bruce E.] Univ Missouri, USDA ARS, Columbia, MO USA. [French, Lee] French Agr Res Inc, Lamberton, MN USA. [Ellersieck, Mark R.] Univ Missouri, Coll Agr Food & Nat Resources Stat, Columbia, MO USA. [French, B. Wade] USDA ARS, Brookings, SD USA. RP Hibbard, BE (reprint author), Univ Missouri, USDA ARS, Columbia, MO USA. EM snzukoff@ksu.edu; ostli001@umn.edu; bpotter@umn.edu; lnm2m9@mail.missouri.edu; azukoff@ksu.edu; lfrench@rrcnet.org; EllersieckM@missouri.edu; wade.french@ars.usda.gov; bruce.hibbard@ars.usda.gov FU Biotechnology Risk Assessment [2009-33120-20256]; USDA-NRI Award [2009-35302-05256]; Syngenta Biotechnology, Inc.; Monsanto Company's Corn Rootworm Knowledge Research Program FX We would like to thank Monsanto Company, DuPont Pioneer, and Syngenta for the seeds used in this study and the farm families of Minnesota for supporting this research through their corn checkoff. Funding, in part, was also provided by Biotechnology Risk Assessment grant 2009-33120-20256, USDA-NRI Award No. 2009-35302-05256, and Syngenta Biotechnology, Inc. A grant from Monsanto Company's Corn Rootworm Knowledge Research Program helped fund portions of the analysis and writing. DowAgroSciences provided diet and protein for the Cry34/35Ab1 diet assays, Monsanto provided diet and protein for the Cry3Bb1 diet assays, and Syngenta Biotechnology provided diet and protein for the mCry3A and eCry3.1Ab assays. We thank three anonymous reviewers for suggestions that have improved the current draft substantially. NR 40 TC 5 Z9 5 U1 6 U2 9 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2016 VL 109 IS 3 BP 1387 EP 1398 DI 10.1093/jee/tow073 PG 12 WC Entomology SC Entomology GA DO8NX UT WOS:000378041400053 ER PT J AU Shrestha, RB Jakka, SRK French, BW Gassmann, AJ AF Shrestha, Ram B. Jakka, Siva R. K. French, B. Wade Gassmann, Aaron J. TI Field-Based Assessment of Resistance to Bt Corn by Western Corn Rootworm (Coleoptera: Chrysomelidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Diabrotica virgifera virgifera; Bt corn; cross-resistance; insect resistance management ID BACILLUS-THURINGIENSIS; TRANSGENIC MAIZE; INSECT RESISTANCE; EVOLVED RESISTANCE; CRY34/35AB1 PROTEINS; EVENT DAS-59122-7; CROPS; SURVIVAL; IMPACT; DIABROTICA AB Western corn rootworm, Diabrotica virgifera virgifera LeConte, is a serious pest of corn and is managed with corn that produces insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt). Beginning in 2009, resistance to Cry3Bb1 corn, and severe injury to Cry3Bb1 corn in the field, was observed in Iowa. However, few data exist on how Cry3Bb1-resistant western corn rootworm interact with various management practices in the field. Using a field experiment, we measured adult emergence and feeding injury to corn roots for both Cry3Bb1-resistant and Cry3Bb1-susceptible populations of western corn rootworm when tested against various Bt corn hybrids and a soil-applied insecticide. Between 2012 and 2013, we evaluated five fields that were associated with greater than one node of feeding injury to Cry3Bb1 corn by western corn rootworm (i.e., problem-field populations), and a laboratory strain that had never been exposed to Bt corn (i.e., control population). Adult emergence for western corn rootworm and root injury to corn were significantly higher in problem-field populations than control populations for both Cry3Bb1 corn and mCry3A corn. By contrast, corn with Cry34/35Ab1, either alone or pyramided with Cry3Bb1, significantly reduced adult emergence and root injury in both problem fields and control fields. In problem fields, application of the soil-applied insecticide to Cry3Bb1 corn significantly reduced root injury, but not adult emergence. Our results are discussed in terms of developing strategies for managing western corn rootworm with resistance to Cry3Bb1 and mCry3A, and delaying the additional evolution of Bt resistance by this pest. C1 [Shrestha, Ram B.; Jakka, Siva R. K.; Gassmann, Aaron J.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. [Jakka, Siva R. K.] Valent USA Corp, MSARC, Leland, MS 38756 USA. [French, B. Wade] USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. RP Shrestha, RB (reprint author), Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. EM shrestrb@iastate.edu; sjakk@valent.com; wade.french@ars.usda.gov; aaronjg@iastate.edu FU Biotechnology Risk Assessment Grant Program competitive grant from the USDA National Institute of Food and Agriculture [2012-33522-20010]; Agricultural Experiment Station at Iowa State University FX We thank M. Dunbar, E. Clifton, K. Masloski, and A. Paolino for helpful comments on this manuscript, and P. Weber for planting research plots. This research was support by Biotechnology Risk Assessment Grant Program competitive grant 2012-33522-20010 from the USDA National Institute of Food and Agriculture, and by the Agricultural Experiment Station at Iowa State University. NR 71 TC 1 Z9 1 U1 6 U2 11 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2016 VL 109 IS 3 BP 1399 EP 1409 DI 10.1093/jee/tow087 PG 11 WC Entomology SC Entomology GA DO8NX UT WOS:000378041400054 ER PT J AU Ajayi-Oyetunde, OO Diers, BW Lagos-Kutz, D Hill, CB Hartman, GL Reuter-Carlson, U Bradley, CA AF Ajayi-Oyetunde, O. O. Diers, B. W. Lagos-Kutz, D. Hill, C. B. Hartman, G. L. Reuter-Carlson, U. Bradley, C. A. TI Differential Reactions of Soybean Isolines With Combinations of Aphid Resistance Genes Rag1, Rag2, and Rag3 to Four Soybean Aphid Biotypes SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Aphis glycines; biotype; soybean ID SINGLE DOMINANT GENE; HEMIPTERA; GENOTYPES; GLYCINES; HOMOPTERA; GERMPLASM; ANTIXENOSIS; VARIABILITY; PESTICIDES; MANAGEMENT AB With the discovery of the soybean aphid (Aphis glycines Matsumura) as a devastating insect pest of soybean (Glycine max (L.) Merr.) in the United States, host resistance was recognized as an important management option. However, the identification of soybean aphid isolates exhibiting strong virulence against aphid resistance genes (Rag genes) has highlighted the need for pyramiding genes to help ensure the durability of host resistance as a control strategy. In this study, soybean isolines with all possible combinations of the resistance and susceptibility alleles at Rag1, Rag2, and Rag3 were evaluated for their effectiveness against the four characterized soybean aphid biotypes. All soybean isolines, including the susceptible check carrying none of the resistance alleles (S1/S2/S3), were infested with each biotype in no-choice greenhouse tests, and the aphid populations developed on each isoline were enumerated 14 d after infestation. All gene combinations, with the exception of Rag3 alone, provided excellent protection against biotype 1. Isolines with Rag2 alone or in combination with Rag1 and Rag3 had greater levels of resistance to biotype 2 than those with either Rag1 alone, Rag3 alone, or the Rag1/3 pyramid. For biotype 3, the Rag1/3 and Rag1/2/3 pyramided lines significantly reduced aphid populations compared with all other gene combinations, while the Rag1/2/3 pyramid provided the greatest protection against biotype 4. Overall, the Rag1/2/3 pyramided line conferred the greatest protection against all four biotypes. C1 [Ajayi-Oyetunde, O. O.; Diers, B. W.; Lagos-Kutz, D.; Hill, C. B.; Hartman, G. L.; Reuter-Carlson, U.; Bradley, C. A.] Univ Illinois, Dept Crop Sci, 1102 S Goodwin Ave, Urbana, IL 61801 USA. [Hill, C. B.] Agricen Sci, 801 S Highway 377, Pilot Point, TX 76258 USA. [Hartman, G. L.] USDA ARS, 1101 W Peabody Dr, Urbana, IL 61801 USA. [Bradley, C. A.] Univ Kentucky, Res & Educ Ctr, Dept Plant Pathol, 1205 Hopkinsville St, Princeton, KY 42445 USA. RP Diers, BW (reprint author), Univ Illinois, Dept Crop Sci, 1102 S Goodwin Ave, Urbana, IL 61801 USA. EM ooajayi2@illinois.edu; bdiers@illinois.edu; dlagos@illinois.edu; curthill@agricensci.com; ghartman@illinois.edu; reuterca@illinois.edu; carl.bradley@uky.edu FU North Central Soybean Research Program; United Soybean Board; Monsanto FX We are grateful to Sarah Schultz for assistance with the TaqMan assay, and we thank Monsanto for support through the graduate student fellowship for O. O. Ajayi-Oyetunde. Funding for the development of the soybean isolines and for biotype maintenance was provided by the North Central Soybean Research Program, and partial support for this project came from the United Soybean Board. NR 54 TC 0 Z9 0 U1 6 U2 7 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2016 VL 109 IS 3 BP 1431 EP 1437 DI 10.1093/jee/tow033 PG 7 WC Entomology SC Entomology GA DO8NX UT WOS:000378041400058 ER PT J AU Fritz, BJ Reisig, DD Sorenson, CE Del Pozo-Valdivia, AI Carter, TE AF Fritz, B. J. Reisig, D. D. Sorenson, C. E. Del Pozo-Valdivia, A. I. Carter, T. E., Jr. TI Host Plant Resistance to Megacopta cribraria (Hemiptera: Plataspidae) in Diverse Soybean Germplasm Maturity Groups V Through VIII SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE kudzu bug; antixenosis; invasive species; Plataspidae; tolerance ID QUANTITATIVE TRAIT LOCI; HETEROPTERA PLATASPIDAE; CORN-EARWORM; REGISTRATION; INSECT; YIELD; PENTATOMIDAE; LINES; LEPIDOPTERA; ANTIBIOSIS AB Initially discovered in Georgia in 2009, the exotic invasive plataspid, Megacopta cribraria (F.), has become a serious pest of soybean (Glycine max (L.) Merrill). Managing M. cribraria in soybean typically involves the application of broad-spectrum insecticides. Soybean host plant resistance is an attractive alternative approach; however, no commercial soybean cultivars have been identified as resistant. During 2013 and 2014, we compared 40 and 44 soybean genotypes, respectively, for resistance to M. cribraria in a split-plot design under natural insect infestation in small-plot experiments. Soybean genotypes were selected to maximize diversity with respect to maturity group, pubescence type, leaf shape, seed size, nitrogen fixation, drought tolerance, seed protein content, and pest resistance. Megacopta cribraria egg masses, nymphs, and adults were counted during the growing season to identify potentially resistant soybean genotypes. Soybean seed yield was measured in insecticide-protected and unprotected conditions to determine tolerance to M. cribraria feeding. In both years, a range of host plant resistance was observed. The fewest M. cribraria adults and nymphs were found on narrow-leaf, small-seeded cultivars 'N7103' and 'Vance,' as well as the nonnodulating cultivar 'Nitrasoy.' Additionally, N7103 and Vance were among the least susceptible genotypes to M. cribraria oviposition in the field. Most 'Benning' cultivar insect-resistant near-isogenic breeding lines also displayed moderate levels of resistance to M. cribraria. Seed yields of Vance and N7103 were less affected by M. cribraria in 2013 than most other soybean genotypes. These results may be useful to soybean breeders to develop cultivars with resistance to M. cribraria. C1 [Fritz, B. J.; Sorenson, C. E.; Del Pozo-Valdivia, A. I.] NCSU, Dept Entomol, 100 Derieux Pl,2301 Gardner Hall, Raleigh, NC 27695 USA. [Carter, T. E., Jr.] USDA ARS, Soybean & Nitrogen Fixat Res, 3127 Ligon St, Raleigh, NC 27607 USA. [Reisig, D. D.] NCSU, Dept Entomol, Vernon James Res & Extens Ctr, 207 Res Stn Rd, Plymouth, NC 27962 USA. RP Fritz, BJ (reprint author), NCSU, Dept Entomol, 100 Derieux Pl,2301 Gardner Hall, Raleigh, NC 27695 USA. EM bjfritz@ncsu.edu; ddreisig@ncsu.edu; clyde_sorenson@ncsu.edu; aidelpoz@ncsu.edu; thomas.carter@ars.usda.gov FU North Carolina Soybean Producers Association; NC Ag Foundation, Inc. FX We sincerely thank Kimberly Porter, Eric Wilbanks, and James Lawrence for assistance with data collection; Robert Masson, Cory Callahan, Dan Mott, and Margarita Villagarcia for assistance in soybean packing, planting, and harvesting; and Jeremy Martin and the Sandhills Research Station staff for assistance with site preparation, deer fencing, and irrigation. This project was funded with the generous support of the North Carolina Soybean Producers Association and a grant from the NC Ag Foundation, Inc. NR 66 TC 1 Z9 1 U1 4 U2 5 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2016 VL 109 IS 3 BP 1438 EP 1449 DI 10.1093/jee/tow036 PG 12 WC Entomology SC Entomology GA DO8NX UT WOS:000378041400059 ER PT J AU Ford, WM Silvis, A Rodrigue, JL Kniowski, AB Johnson, JB AF Ford, W. Mark Silvis, Alexander Rodrigue, Jane L. Kniowski, Andrew B. Johnson, Joshua B. TI Deriving Habitat Models for Northern Long-Eared Bats from Historical Detection Data: A Case Study Using the Fernow Experimental Forest SO JOURNAL OF FISH AND WILDLIFE MANAGEMENT LA English DT Article DE acoustical sampling; day roosts; distribution model; habitat model; landscape distribution; maximum-likelihood modeling; mist-net sampling; Myotis septentrionalis; northern long-eared bat ID SEPTENTRIONALIS MATERNITY COLONIES; MYOTIS MYOTIS-SEPTENTRIONALIS; WHITE-NOSE SYNDROME; WEST-VIRGINIA; WIND FARMS; IMPACT; CHIROPTERA; ROOSTS AB The listing of the northern long-eared bat (Myotis septentrionalis) as federally threatened under the Endangered Species Act following severe population declines from white-nose syndrome presents considerable challenges to natural resource managers. Because the northern long-eared bat is a forest habitat generalist, development of effective conservation measures will depend on appropriate understanding of its habitat relationships at individual locations. However, severely reduced population sizes make gathering data for such models difficult. As a result, historical data may be essential in development of habitat models. To date, there has been little evaluation of how effective historical bat presence data, such as data derived from mist-net captures, acoustic detection, and day-roost locations, may be in developing habitat models, nor is it clear how models created using different data sources may differ. We explored this issue by creating presence probability models for the northern long-eared bat on the Fernow Experimental Forest in the central Appalachian Mountains of West Virginia using a historical, presence-only data set. Each presence data type produced outputs that were dissimilar but that still corresponded with known traits of the northern long-eared bat or are easily explained in the context of the particular data collection protocol. However, our results also highlight potential limitations of individual data types. For example, models from mist-net capture data only showed high probability of presence along the dendritic network of riparian areas, an obvious artifact of sampling methodology. Development of ecological niche and presence models for northern long-eared bat populations could be highly valuable for resource managers going forward with this species. We caution, however, that efforts to create such models should consider the substantial limitations of models derived from historical data, and address model assumptions. C1 [Ford, W. Mark] Virginia Polytech Inst & State Univ, Virginia Cooperat Fish & Wildlife Res Unit 0321, US Geol Survey, 310 W Campus Dr, Blacksburg, VA 24061 USA. [Silvis, Alexander; Kniowski, Andrew B.] Virginia Polytech Inst & State Univ, Dept Fish & Wildlife Conservat 0321, 310 W Campus Dr, Blacksburg, VA 24061 USA. [Rodrigue, Jane L.] US Forest Serv, No Res Stn, Princeton, WV 24740 USA. [Rodrigue, Jane L.] METI Inc, 230 Sunny Ridge Rd, Pearisburg, VA 24134 USA. [Johnson, Joshua B.] Pennsylvania Game Commiss, 2001 Elmerton Ave, Harrisburg, PA 17110 USA. RP Silvis, A (reprint author), Virginia Polytech Inst & State Univ, Dept Fish & Wildlife Conservat 0321, 310 W Campus Dr, Blacksburg, VA 24061 USA. EM silvis@vt.edu FU U.S. Forest Service Northern Research Station; U.S. Geological Survey; Cooperative Research Unit Program; Virginia Tech Department of Fish and Wildlife Conservation FX U.S. Forest Service Northern Research Station, U.S. Geological Survey, Cooperative Research Unit Program, and Virginia Tech Department of Fish and Wildlife Conservation provided financial support for this project. Field assistance was provided by D. Lowther and R. Hovatter. We thank C. Johnson for numerous discussions about northern long-eared bat ecology that ultimately led to this research endeavor. We thank the three anonymous reviewers for their helpful comments. NR 45 TC 0 Z9 0 U1 15 U2 23 PU U S FISH & WILDLIFE SERVICE PI SHEPHERDSTOWN PA NATL CONSERVATION TRAINING CENTER, CONSERVATION LIBRARY, 698 CONSERVATION WAY, SHEPHERDSTOWN, WV 25443 USA SN 1944-687X J9 J FISH WILDL MANAG JI J. Fish Wildl. Manag. PD JUN PY 2016 VL 7 IS 1 BP 86 EP 98 DI 10.3996/012015-JFWM-004 PG 13 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DO9CT UT WOS:000378082600008 ER PT J AU Albers, G Edwards, JW Rogers, RE Mastro, LL AF Albers, Geriann Edwards, John W. Rogers, Richard E. Mastro, Lauren L. TI Natality of Yearling Coyotes in West Virginia SO JOURNAL OF FISH AND WILDLIFE MANAGEMENT LA English DT Article DE coyote; Canis latrans; reproduction; West Virginia ID CANIS-LATRANS; REPRODUCTIVE-BIOLOGY; BODY CONDITION; POPULATION; DEMOGRAPHY; DENSITY AB Minimal information is available regarding the reproduction of coyotes (Canis latrans) in the Mid-Atlantic region, which includes the states of Delaware, Maryland, North Carolina, Pennsylvania, Virginia, and West Virginia. Since reproductive information is useful to assess populations and determine management strategies and because this information is unavailable for the Mid-Atlantic, we examined uterine tracts of 66 female coyotes collected from February to May 2010 for fetuses. We measured fetuses using a digital caliper and approximated dates of conception and parturition. Nine (13.6%) female coyotes were pregnant with visible fetuses; seven of these females were yearlings (1.5-2.5 y old). Average litter size of yearling coyotes was 5.4 (SE = 0.48). We estimated an average conception date of 2 February and an average parturition date of 6 April. Conception and parturition dates were within the reported range for coyotes, though one female successfully bred in early January, which is earlier than reported in the literature. The relatively high proportion (30.4%) of yearling females breeding in West Virginia may reflect abundant food resources, low density of coyotes, increasing human-caused mortality, or a combination of these factors. Reproductive information reported here will be helpful in monitoring coyote population trends and in the assessment of management strategies. C1 [Albers, Geriann; Edwards, John W.] W Virginia Univ, Div Forestry & Nat Resources, 322 Percival Hall, Morgantown, WV 26505 USA. [Rogers, Richard E.] West Virginia Div Nat Resources, 1 Depot St, Romsey 26757, WV, England. [Mastro, Lauren L.] Utah State Univ, USDA, Natl Wildlife Res Ctr, Wildlife Serv, 163 BNR Bldg, Logan, UT 84322 USA. RP Albers, G (reprint author), W Virginia Univ, Div Forestry & Nat Resources, 322 Percival Hall, Morgantown, WV 26505 USA. EM geriann.albers@gmail.com FU West Virginia Division of Natural Resources; Federal Wildlife and Sport Fish Restoration Program, West Virginia University Division of Forestry and Natural Resources, U.S. Department of Agriculture Animal and Plant Health Inspection Service Wildlife Services; Berryman Institute FX This work was funded in part by West Virginia Division of Natural Resources and the Federal Wildlife and Sport Fish Restoration Program, West Virginia University Division of Forestry and Natural Resources, U.S. Department of Agriculture Animal and Plant Health Inspection Service Wildlife Services, and The Berryman Institute. Thank you to all our volunteers, interns, and technicians, especially L. C. Moon. We also thank the West Virginia Trappers Association, Wildlife Services personnel, and all the personnel at the West Virginia Divison of Natural Resources who aided in sample collection, as well as J. L. Berl, M. D. Jones, A. N. Tri, the Associate Editor, and two anonymous reviewers for critical review of earlier drafts. NR 41 TC 0 Z9 0 U1 0 U2 0 PU U S FISH & WILDLIFE SERVICE PI SHEPHERDSTOWN PA NATL CONSERVATION TRAINING CENTER, CONSERVATION LIBRARY, 698 CONSERVATION WAY, SHEPHERDSTOWN, WV 25443 USA SN 1944-687X J9 J FISH WILDL MANAG JI J. Fish Wildl. Manag. PD JUN PY 2016 VL 7 IS 1 BP 192 EP 197 DI 10.3996/072015-JFWM-063 PG 6 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DO9CT UT WOS:000378082600019 ER PT J AU Harrison, RL Rowley, DL Keena, MA AF Harrison, Robert L. Rowley, Daniel L. Keena, Melody A. TI Geographic isolates of Lymantria dispar multiple nucleopolyhedrovirus: Genome sequence analysis and pathogenicity against European and Asian gypsy moth strains SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Baculovirus; Nucleopolyhedrovirus; Gypsy moth; Asian gypsy moth; Lymantria dispar; LdMNPV ID NUCLEAR POLYHEDROSIS-VIRUS; BOMBYX-MORI NUCLEOPOLYHEDROVIRUS; REPUBLIC-OF-CHINA; NORTH-AMERICA; LEPIDOPTERA LYMANTRIIDAE; ORGYIA-PSEUDOTSUGATA; GENETIC-VARIATION; BRO; BACULOVIRUSES; ALIGNMENT AB Isolates of the baculovirus species Lymantria dispar multiple nucleopolyhedrovirus have been formulated and applied to suppress outbreaks of the gypsy moth, L dispar. To evaluate the genetic diversity in this species at the genomic level, the genomes of three isolates from Massachusetts, USA (LdMNPV-Ab-a624), Spain (LdMNPV-3054), and Japan (LdMNPV-3041) were sequenced and compared with four previously determined LdMNPV genome sequences. The LdMNPV genome sequences were collinear and contained the same homologous repeats (hrs) and clusters of baculovirus repeat orf (bro) gene family members in the same relative positions in their genomes, although sequence identities in these regions were low. Of 146 non-bro ORFs annotated in the genome of the representative isolate LdMNPV 5-6,135 ORFs were found in every other LdMNPV genome, including the 37 core genes of Baculoviridae and other genes conserved in genus Alphabaculovirus. Phylogenetic inference with an alignment of the core gene nucleotide sequences grouped isolates 3041 (Japan) and 2161 (Korea) separately from a cluster containing isolates from Europe, North America, and Russia. To examine phenotypic diversity, bioassays were carried out with a selection of isolates against neonate larvae from three European gypsy moth (Lymantria dispar dispar) and three Asian gypsy moth (Lymantria dispar asiatica and Lymantria dispar japonica) colonies. LdMNPV isolates 2161 (Korea), 3029 (Russia), and 3041 (Japan) exhibited a greater degree of pathogenicity against all L. dispar strains than LdMNPV from a sample of Gypchek. This study provides additional information on the genetic diversity of LdMNPV isolates and their activity against the Asian gypsy moth, a potential invasive pest of North American trees and forests. Published by Elsevier Inc. C1 [Harrison, Robert L.; Rowley, Daniel L.] USDA ARS, Invas Insect Biocontrol & Behav Lab, Beltsville Agr Res Ctr, 10300 Baltimore Ave, Beltsville, MD 20705 USA. [Keena, Melody A.] US Forest Serv, No Res Stn, USDA, 51 Mill Pond Rd, Hamden, CT 06514 USA. RP Harrison, RL (reprint author), USDA ARS, Invas Insect Biocontrol & Behav Lab, Beltsville Agr Res Ctr, BARC W, Bldg 007,Room 301,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Robert.L.Harrison@ars.usda.gov; Daniel.Rowley@ars.usda.gov; mkeena@fs.fed.us FU U.S. Department of Agriculture FX We wish to thank Daniel Barakh and Pallavi Thapa (USDA-ARS, Beltsville, MD) and Paul Moore (USDA-FS, Ansonia, CT) for assistance with bioassays, and Renee Lapointe (Northern Forestry Centre, Natural Resources Canada) for providing a sample of Gypchek produced by Sylvar Technologies Inc. This work was supported by the U.S. Department of Agriculture. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 59 TC 1 Z9 1 U1 3 U2 5 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 EI 1096-0805 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD JUN PY 2016 VL 137 BP 10 EP 22 DI 10.1016/j.jip.2016.03.014 PG 13 WC Zoology SC Zoology GA DP2EL UT WOS:000378300800002 PM 27090923 ER PT J AU Hwang, IH Wicklow, DT Gloer, JB AF Hwang, In Hyun Wicklow, Donald T. Gloer, James B. TI New punctaporonins from two fungicolous isolates of Pestalotiopsis sp. SO PHYTOCHEMISTRY LETTERS LA English DT Article DE Caryophyllene; Sesquiterpenoids; Fungicolous; Fungi; Pestalotiopsis; Punctaporonins ID FUNGUS PORONIA-PUNCTATA; ALLYLIC ALCOHOLS; CARYOPHYLLENE; SESQUITERPENOIDS; METABOLITES AB Seven new caryophyllene sesquiterpenoids (5-11) were isolated during investigation of two fungicolous isolates of Pestalotiopsis sp. and identified as punctaporonin analogues. The structures of these compounds were mainly assigned by analysis of NMR and HRMS data. Compounds 5-7 are 13-hydroxy analogues of 6-hydroxypunctaporonins A, B, and E, respectively, and compound 8 is a 6-hydroxy analogue of punctaporonin D. Compounds 9 and 11 are ketone-containing analogues of 6-hydroxypunctaporonin A and fuscoatrol A, respectively, and compound 10 is a 9-methoxy derivative of 8. The names punctaporonins N, O, and P are proposed for compounds 9, 10, and 11, respectively. Antibacterial activity associated with the extract was attributed to the presence of known punctaporonins as major metabolites. (C) 2016 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved. C1 [Hwang, In Hyun; Gloer, James B.] Univ Iowa, Dept Chem, Iowa City, IA 52242 USA. [Wicklow, Donald T.] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Gloer, JB (reprint author), Univ Iowa, Dept Chem, Iowa City, IA 52242 USA. EM james-gloer@uiowa.edu FU National Science Foundation [CHE-1011847]; NIH [S10 RR025500]; NSF [CHE-0946779] FX Financial support was provided in part by a grant from the National Science Foundation (CHE-1011847). Acquisition of the 600 MHz NMR and HRMS instruments employed in this work was supported by grants from NIH (S10 RR025500) and NSF (CHE-0946779), respectively. Technical assistance from staff of the University of Iowa NMR and MS facilities is greatly appreciated. The USDA is an equal opportunity provider and employer. NR 16 TC 0 Z9 0 U1 2 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1874-3900 EI 1876-7486 J9 PHYTOCHEM LETT JI Phytochem. Lett. PD JUN PY 2016 VL 16 BP 257 EP 262 DI 10.1016/j.phytol.2016.05.001 PG 6 WC Plant Sciences; Oncology SC Plant Sciences; Oncology GA DO8IQ UT WOS:000378027300046 ER PT J AU Seyfried, M Link, T Marks, D Murdock, M AF Seyfried, Mark Link, Timothy Marks, Danny Murdock, Mark TI Soil Temperature Variability in Complex Terrain Measured Using Fiber-Optic Distributed Temperature Sensing SO VADOSE ZONE JOURNAL LA English DT Article ID AIR-TEMPERATURE; WATER DYNAMICS; UNITED-STATES; MODEL; PATTERNS; CARBON; IDAHO; MANIPULATION; CAPABILITIES; CALIBRATION AB Soil temperature (T-s) exerts critical controls on hydrologic and biogeochemical processes, but the magnitude and nature of T-s variability in a landscape setting are rarely documented. Fiber-optic distributed temperature sensing (DTS) systems potentially measure T-s at high density across a large extent. A fiber-optic cable 771 m long was installed at a depth of 10 cm in contrasting landscape units (LUs) defined by vegetative cover at Upper Sheep Creek in the Reynolds Creek Experimental Watershed (RCEW) and Critical Zone Observatory in Idaho. The purpose was to evaluate the applicability of DTS in remote settings and to characterize Ts variability in complex terrain. Measurement accuracy was similar to other field instruments (+/- 0.4 degrees C), and T-s changes of approximately 0.05 degrees C at a monitoring spatial scale of 1 m were resolved with occasional calibration and an ambient temperature range of 50 degrees C. Differences in solar inputs among LUs were strongly modified by surface conditions. During spatially continuous snow cover, T-s was practically homogeneous across LUs. In the absence of snow cover, daily average T-s was highly variable among LUs due to variations in vegetative cover, with a standard deviation (SD) greater than 5 degrees C, and relatively uniform (SD <1.5 degrees C) within LUs. Mean annual soil temperature differences among LUs of 5.2 degrees C was greater than those of 4.4 degrees C associated with a 910-m elevation difference within the RCEW. In this environment, effective T-s simulation requires representation of relatively small-scale (< 20 m) LUs due to the deterministic spatial variability of T-s. C1 [Seyfried, Mark; Marks, Danny; Murdock, Mark] USDA ARS, Boise, ID 83712 USA. [Link, Timothy] Univ Idaho, Dept Forest Rangeland & Fire Sci, Moscow, ID 83844 USA. RP Seyfried, M (reprint author), USDA ARS, Boise, ID 83712 USA. EM mark.seyfried@ars.usda.gov NR 62 TC 1 Z9 1 U1 7 U2 8 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD JUN PY 2016 VL 15 IS 6 DI 10.2136/vzj2015.09.0128 PG 18 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA DP2PX UT WOS:000378332500002 ER PT J AU Wang, Y Ostlund, EN Jun, Y Nie, FP Li, YG Johnson, DJ Lin, R Li, ZG AF Wang, Yu Ostlund, Eileen N. Jun, Yang Nie, Fu-ping Li, Ying-guo Johnson, Donna J. Lin, Rui Li, Zheng-guo TI Combining reverse-transcription multiplex PCR and microfluidic electrophoresis to simultaneously detect seven mosquito-transmitted zoonotic encephalomyelitis viruses SO VETERINARY JOURNAL LA English DT Article DE Mosquito-transmitted viruses; Encephalomyelitis viruses; Microfluidic; Multiplex PCR ID WEST-NILE-VIRUS; POLYMERASE-CHAIN-REACTION; REAL-TIME PCR; EQUINE ENCEPHALITIS VIRUSES; CULEX-TARSALIS DIPTERA; RT-PCR; NUCLEIC-ACID; DIAGNOSIS; DISEASE; CULICIDAE AB Several mosquito-transmitted viruses are causative agents for zoonotic encephalomyelitis. Rapid identification of these viruses in mosquito populations is an effective method for surveying these diseases. To detect multiple mosquito-transmitted viral agents, including West Nile virus, Saint Louis encephalitis virus, Venezuelan equine encephalomyelitis virus, Western equine encephalomyelitis virus, Eastern equine encephalomyelitis virus, Highlands J virus and Japanese encephalitis virus, an assay using multiplex reverse-transcription PCR combined with microfluidic electrophoresis was developed and evaluated. Tailed nested primers were used in the assay to amplify specific viral genomic segments, and products with specific length were further analyzed by using a microfluidic electrophoresis chip. The assay exhibited good specificity and analytical sensitivity (10(2) copies/mu L). This technology can be helpful in the quarantine and surveillance of exotic encephalomyelitis viruses which are transmitted by mosquitoes. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Wang, Yu; Li, Ying-guo; Lin, Rui; Li, Zheng-guo] Chongqing Univ, Sch Life Sci, Chongqing 400030, Peoples R China. [Wang, Yu; Jun, Yang; Nie, Fu-ping; Li, Ying-guo] Chongqing Entry Inspect & Quarantine Bur, Engn Res Ctr Import & Export Food Safety, Ctr Tech, Chongqing 400020, Peoples R China. [Ostlund, Eileen N.; Johnson, Donna J.] STAS VS APHIS USDA, Diagnost Virol Lab, Natl Vet Serv Labs, Ames, IA 50010 USA. RP Li, ZG (reprint author), Chongqing Univ, Sch Life Sci, Chongqing 400030, Peoples R China. EM zhengguoli@cqu.edu.cn RI Li, Zhengguo/A-5572-2017 FU National High Technology Research and Development Program of China [2012AA101702]; AQSIQ [2012IK029, 2009IK014] FX This work was supported by the National High Technology Research and Development Program of China (2012AA101702) and AQSIQ projects (2012IK029, 2009IK014). NR 59 TC 0 Z9 0 U1 1 U2 5 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1090-0233 EI 1532-2971 J9 VET J JI Vet. J. PD JUN PY 2016 VL 212 BP 27 EP 35 DI 10.1016/j.tvjl.2015.10.040 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA DP0PZ UT WOS:000378193000006 PM 27256022 ER PT J AU Yue, FX Lu, FC Ralph, S Ralph, J AF Yue, Fengxia Lu, Fachuang Ralph, Sally Ralph, John TI Identification of 4-O-5-Units in Softwood Lignins via Definitive Lignin Models and NMR SO BIOMACROMOLECULES LA English DT Article ID MILLED WOOD LIGNIN; SOLUTION-STATE NMR; COMPRESSION WOOD; DFRC METHOD; THIOACIDOLYSIS; LIGNIFICATION; BIOSYNTHESIS; CHEMISTRY; IMPROVES; SPRUCE AB Lignins are complex and heterogeneous natural polymers in which the major units are characterized by certain prominent interunit linkages. Previous attempts to identify and quantify 4-O-5-linked units in softwood lignins by NMR were not successful. In this work, various lignin model compounds, including the tetramers formed by the 4-O-5-coupling of beta-O-4-, beta-beta, and beta-5-model dimers, were synthesized. Such compounds are better able to model the corresponding structures in lignins than those used previously. 4-O-5-Linked structures could be dearly observed and identified in real softwood lignin samples by comparison of their 2D HSQC NMR spectra with those from the model compounds. When comparing NMR data of phenol-acetylated versus phenoletherified model compounds with those of acetylated lignins, it was apparent that most of the 4-O-5-linked structures in softwood lignins are present as free-phenolic end units. C1 [Yue, Fengxia; Lu, Fachuang] S China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Guangdong, Peoples R China. [Yue, Fengxia; Lu, Fachuang; Ralph, John] Univ Wisconsin, Dept Biochem, Madison, WI 53726 USA. [Yue, Fengxia; Lu, Fachuang; Ralph, John] Univ Wisconsin, DOE Great Lakes Bioenergy Res Ctr, Wisconsin Energy Inst, Madison, WI 53726 USA. [Ralph, Sally] USDA, Forest Prod Lab, Madison, WI 53706 USA. RP Lu, FC (reprint author), S China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Guangdong, Peoples R China.; Lu, FC (reprint author), Univ Wisconsin, Dept Biochem, Madison, WI 53726 USA.; Lu, FC (reprint author), Univ Wisconsin, DOE Great Lakes Bioenergy Res Ctr, Wisconsin Energy Inst, Madison, WI 53726 USA. EM fachuanglu@wisc.edu FU China Scholarship Council, State Education Department; DOE Great Lakes Bioenergy Research Center (DOE BER Office of Science) [DE-FC02-07ER64494]; Stanford University's Global Climate and Energy Project FX The authors would like to thank the China Scholarship Council, State Education Department, for supporting F.Y. as a visiting student in the Department of Biochemistry, the University of Wisconsin, and at the Great Lakes Bioenergy Research Center (GLBRC). This work was funded by the DOE Great Lakes Bioenergy Research Center (DOE BER Office of Science, DE-FC02-07ER64494) and in part by Stanford University's Global Climate and Energy Project. NR 38 TC 2 Z9 2 U1 15 U2 39 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1525-7797 EI 1526-4602 J9 BIOMACROMOLECULES JI Biomacromolecules PD JUN PY 2016 VL 17 IS 6 BP 1909 EP 1920 DI 10.1021/acs.biomac.6b00256 PG 12 WC Biochemistry & Molecular Biology; Chemistry, Organic; Polymer Science SC Biochemistry & Molecular Biology; Chemistry; Polymer Science GA DO6WS UT WOS:000377924800001 PM 27078826 ER PT J AU Lange, MD Beck, BH Brown, JD Farmer, BD Barnett, LM Webster, CD AF Lange, Miles D. Beck, Benjamin H. Brown, Jason D. Farmer, Bradley D. Barnett, L. Matthew Webster, Carl D. TI Missing the target: DNAk is a dominant epitope in the humoral immune response of channel catfish (Ictalurus punctatus) to Flavobacterium columnare SO FISH & SHELLFISH IMMUNOLOGY LA English DT Meeting Abstract DE Sea lice; vaccine; immune response; cytokines; ribosomal protein C1 [Lange, Miles D.; Beck, Benjamin H.; Brown, Jason D.; Farmer, Bradley D.; Barnett, L. Matthew; Webster, Carl D.] ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1050-4648 EI 1095-9947 J9 FISH SHELLFISH IMMUN JI Fish Shellfish Immunol. PD JUN PY 2016 VL 53 SI SI MA O-017 BP 64 EP 64 PG 1 WC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences GA DO4CW UT WOS:000377730400024 ER PT J AU Hansen, JD Woodson, JC Deas, E McPherson, V Welch, TJ AF Hansen, John D. Woodson, James C. Deas, Emma McPherson, Victoria Welch, Timothy J. TI An outbreak of Plesiomonas shigelloides in zebrafish SO FISH & SHELLFISH IMMUNOLOGY LA English DT Meeting Abstract DE Zebrafish; Plesiomonas; Inflammation; Disease; Antibiotics C1 [Hansen, John D.; Woodson, James C.] US Geol Survey, Western Fisheries Res Ctr, Seattle, WA USA. [Hansen, John D.; Deas, Emma; McPherson, Victoria] Univ Washington, Dept Global Hlth, Seattle, WA 98195 USA. [Welch, Timothy J.] ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, Kearneysville, WV 25430 USA. EM jhansen@usgs.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1050-4648 EI 1095-9947 J9 FISH SHELLFISH IMMUN JI Fish Shellfish Immunol. PD JUN PY 2016 VL 53 SI SI MA O-003 BP 95 EP 96 PG 2 WC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences GA DO4CW UT WOS:000377730400114 ER PT J AU Moore, C Wiens, G Zwollo, P AF Moore, Catherine Wiens, Greg Zwollo, Patty TI B/Myeloid cell signatures in Flavobacterium psychrophilum-resistant and -susceptible genetic lines of rainbow trout SO FISH & SHELLFISH IMMUNOLOGY LA English DT Meeting Abstract DE BCWD; Transcription factors; B cell development; Myeloid development; Rainbow trout C1 [Moore, Catherine; Zwollo, Patty] Coll William & Mary, Dept Biol, Williamsburg, VA 23185 USA. [Wiens, Greg] ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, Kearneysville, WV 25430 USA. EM pxzwol@wm.edu NR 0 TC 0 Z9 0 U1 1 U2 1 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1050-4648 EI 1095-9947 J9 FISH SHELLFISH IMMUN JI Fish Shellfish Immunol. PD JUN PY 2016 VL 53 SI SI MA O-0010 BP 97 EP 98 PG 2 WC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences GA DO4CW UT WOS:000377730400121 ER PT J AU Xu, DH Klesius, PH AF Xu, De-Hai Klesius, Phillip H. TI Evaluation of immune responses against the parasite Ichthyophthirius multifiliis between channel catfish and hybrid catfish SO FISH & SHELLFISH IMMUNOLOGY LA English DT Meeting Abstract DE Immune protection; Vaccination; Parasite; Infection; Survival C1 [Xu, De-Hai; Klesius, Phillip H.] ARS, USDA, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 USA. EM dehai.xu@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1050-4648 EI 1095-9947 J9 FISH SHELLFISH IMMUN JI Fish Shellfish Immunol. PD JUN PY 2016 VL 53 SI SI MA O-042 BP 108 EP 108 PG 1 WC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences GA DO4CW UT WOS:000377730400153 ER PT J AU McHugh, T AF McHugh, Tara TI Living and Learning Food Processing SO FOOD TECHNOLOGY LA English DT Article C1 [McHugh, Tara] ARS, USDA, Albany, CA 94710 USA. RP McHugh, T (reprint author), ARS, USDA, Albany, CA 94710 USA. EM tara.mchugh@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU INST FOOD TECHNOLOGISTS PI CHICAGO PA 525 WEST VAN BUREN, STE 1000, CHICAGO, IL 60607-3814 USA SN 0015-6639 J9 FOOD TECHNOL-CHICAGO JI Food Technol. PD JUN PY 2016 VL 70 IS 6 BP 130 EP 135 PG 6 WC Food Science & Technology SC Food Science & Technology GA DO5UK UT WOS:000377848100017 ER PT J AU Ladely, SR Meinersmann, RJ Ball, TA Fedorka-Cray, PJ AF Ladely, Scott R. Meinersmann, Richard J. Ball, Takiyah A. Fedorka-Cray, Paula J. TI Antimicrobial Susceptibility and Plasmid Replicon Typing of Salmonella enterica Serovar Kentucky Isolates Recovered from Broilers SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID SEROTYPE ENTERITIDIS INFECTIONS; ESCHERICHIA-COLI; FOODNET SITES; RESISTANCE; POULTRY AB Salmonella Kentucky has become the predominant serovar recovered frombroilers slaughtered in the United States, and the prevalence of antimicrobial resistance (AMR) has increased dramatically in this serovar. Relationships between AMR, genotype, and plasmid replicon types were characterized for 600 Salmonella Kentucky isolates recovered from chicken carcasses from 2004 to 2013. Pulsed-field gel electrophoresis cluster analysis revealed 112 unique types sharing 79% similarity. Over half of the isolates studies were assigned to two large clusters (unique restriction patterns) consisting of 190 (A) and 151 (B) isolates. The remaining (n = 259) more diverse isolates (110 unique patterns) shall be designated cluster C for discussion. Clusters A had significantly more (p < 0.05) isolates resistant to streptomycin (68.4%) and tetracycline (91.6%) compared to cluster C (50.6% and 40.9% to streptomycin and tetracycline, respectively) or cluster B, which had the least (p < 0.05) resistance (11.9% and 13.2% to streptomycin and tetracycline, respectively). In addition, there was segregation of plasmid replicon types among clusters. Cluster A had significantly more (p < 0.05) replicon type FIB (90.5%) compared to cluster C (37.1%), which had significantly more compared to cluster B (10.6%). Cluster B had significantly more (p < 0.05) replicon type I1 (87.4%) compared to cluster C (68.7%), which had significantly more (p < 0.05) compared to cluster A (32.6%). Cluster C harbored significantly more (p < 0.05) HI2 replicon type (18.1%) compared to clonal clusters A (1.6%) or B (1.3%). The prevalence of plasmid replicon type A/C did not differ among clusters (A, 0.5%; B, 2.0%; C, 0.4%). Both streptomycin and tetracycline resistance were significantly linked (p < 0.05) to plasmid replicon type FIB. In addition, replicon type HI2 was also significantly linked (p < 0.05) to streptomycin resistance. We conclude that the dramatic increase in streptomycin and tetracycline resistance among Salmonella Kentucky isolated frompoultry is due to the expansion of strains harboring plasmid replicon types FIB and HI2. C1 Food Safety Inspect Serv, Outbreaks Sect, Eastern Lab, Richard B Russell Agr Res Ctr,USDA, Athens, GA USA. [Meinersmann, Richard J.; Ball, Takiyah A.] ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, Richard B Russell Agr Res Ctr, USDA, 950 Coll Stn Rd, Athens, GA 30605 USA. [Fedorka-Cray, Paula J.] N Carolina State Univ, Coll Vet Med, Dept Populat Hlth & Pathobiol, Raleigh, NC USA. RP Meinersmann, RJ (reprint author), ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, Richard B Russell Agr Res Ctr, USDA, 950 Coll Stn Rd, Athens, GA 30605 USA. EM rick.meinersmann@ars.usda.gov NR 15 TC 1 Z9 1 U1 2 U2 2 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 EI 1556-7125 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD JUN PY 2016 VL 13 IS 6 BP 309 EP 315 DI 10.1089/fpd.2015.2102 PG 7 WC Food Science & Technology SC Food Science & Technology GA DO6IR UT WOS:000377887200005 PM 27028167 ER PT J AU McDaniel, VL Perry, RW Koerth, NE Guldin, JM AF McDaniel, Virginia L. Perry, Roger W. Koerth, Nancy E. Guldin, James M. TI Evaluation of FOFEM Fuel Loads and Consumption Estimates in Pine-Oak Forests and Woodlands of the Ouachita Mountains in Arkansas, USA SO FOREST SCIENCE LA English DT Article DE fire effects models; fire emissions; Fuel Characteristic Classification System; shortleaf pine ID WILDLAND FIRE EMISSIONS; PRESCRIBED FIRES; HARDWOOD FORESTS; SMOKE EMISSIONS; UNITED-STATES; SEVERITY; SYSTEM; RESTORATION; MODELS; CARBON AB Accurate fuel load and consumption predictions are important to estimate fire effects and air pollutant emissions. The FOFEM (First Order Fire Effects Model) is a commonly used model developed in the western United States to estimate fire effects such as fuel consumption, soil heating, air pollutant emissions, and tree mortality. However, the accuracy of the model in the eastern United States has not been well tested. As a result, managers are turning to locally collected data sets from eastern forests to improve the accuracy of FOFEM and other models. FOFEM lacks local fuel load and consumption data for the Ouachita Mountains, an area with nearly 50,000 ha prescribe burned annually. In this study, we compared fuel loads and consumption using field-collected data with data predicted by FOFEM. We determined fuel loads before and after 15 prescribed fires by sampling live fuels, down woody debris, litter, and duff in three cover types (oak forest, pine-oak forest, and pine woodland) on the Ouachita National Forest in Arkansas. Default estimates of litter and duff fuel load in FOFEM were up to 346 and 1,307% greater than field estimates, respectively. FOFEM estimates of 10-hour, 1-hour, and litter fuel consumption were up to 182, 150, and 46% greater, respectively, than field-measured consumption. These overestimations of fuel load and consumption could result in overpredictions of air pollutant emissions and reduce the area of habitat restored and maintained by prescribed burning as fire managers seek to comply with air quality standards. C1 [McDaniel, Virginia L.; Perry, Roger W.; Koerth, Nancy E.; Guldin, James M.] US Forest Serv, USDA, Southern Res Stn, Hot Springs, AR USA. RP McDaniel, VL (reprint author), US Forest Serv, USDA, Southern Res Stn, Hot Springs, AR USA. EM vmcdaniel@fs.fed.us; rperry03@fs.fed.us; nkoerth@fs.fed.us; jguldin@fs.fed.us FU Ouachita National Forest FX This project would never have been completed without the invaluable guidance and encouragement of Becky Finzer. We thank Adam Strothers, Doug Sprouse, Kenny Frick, Tim Nutley, R.L. Self, Rusty Oglesby, Roger Fryar, Chad Yocum, Mary and Carl McDaniel, Susan Hooks, Clay Knight, Jason Morris, Stan Wagner, Debbie Shea, James Silverstone, Many Spetich, Shawn Cochran, Nick Finzer, Beth Buchanan, Judy Logan, Andy Dyer, Darrell Barlow, Jennifer Benefield, and Georgia James for assisting with data collection, accomplishing the prescribed burns, and providing guidance on the project. We thank the Ouachita National Forest for supporting this project. Duncan Lutes and Susan Prichard provided encouragement and a thoughtful critique of the article. We also appreciated the critical review and recommendations of four anonymous reviewers whose suggestions greatly improved this article. NR 58 TC 0 Z9 0 U1 2 U2 2 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD JUN PY 2016 VL 62 IS 3 BP 307 EP 315 DI 10.5849/forsci.15-085 PG 9 WC Forestry SC Forestry GA DO8FD UT WOS:000378017100007 ER PT J AU Ipcho, S Sundelin, T Erbs, G Kistler, HC Newman, MA Olsson, S AF Ipcho, Simon Sundelin, Thomas Erbs, Gitte Kistler, H. Corby Newman, Mari-Anne Olsson, Stefan TI Fungal Innate Immunity Induced by Bacterial Microbe-Associated Molecular Patterns (MAMPs) SO G3-GENES GENOMES GENETICS LA English DT Article DE fungal-bacterial interaction; innate immunity; MAMPs; transcriptomics ID FUSARIUM-GRAMINEARUM; SACCHAROMYCES-CEREVISIAE; ASPERGILLUS-NIDULANS; RHIZOCTONIA-SOLANI; IRON-METABOLISM; PLANT; ANIMALS; RECOGNITION; EXPRESSION; INFECTION AB Plants and animals detect bacterial presence through Microbe-Associated Molecular Patterns (MAMPs) which induce an innate immune response. The field of fungal-bacterial interaction at the molecular level is still in its infancy and little is known about MAMPs and their detection by fungi. Exposing Fusarium graminearum to bacterial MAMPs led to increased fungal membrane hyperpolarization, a putative defense response, and a range of transcriptional responses. The fungus reacted with a different transcript profile to each of the three tested MAMPs, although a core set of genes related to energy generation, transport, amino acid production, secondary metabolism, and especially iron uptake were detected for all three. Half of the genes related to iron uptake were predicted MirA type transporters that potentially take up bacterial siderophores. These quick responses can be viewed as a preparation for further interactions with beneficial or pathogenic bacteria, and constitute a fungal innate immune response with similarities to those of plants and animals. C1 [Ipcho, Simon; Sundelin, Thomas; Erbs, Gitte; Newman, Mari-Anne; Olsson, Stefan] Univ Copenhagen, Dept Plant & Environm Sci PLEN, DK-1871 Frederiksberg C, Denmark. [Kistler, H. Corby] USDA ARS, Cereal Dis Lab, St Paul, MN USA. RP Olsson, S (reprint author), Univ Copenhagen, Dept Plant & Environm Sci, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark. EM sto@plen.ku.dk RI Newman, Mari-Anne/M-1550-2013; OI Newman, Mari-Anne/0000-0001-5782-6071; Olsson, Stefan/0000-0003-1931-9081; Erbs, Gitte/0000-0003-3764-6473 FU Minnesota Supercomputing Institute; Villum Foundation, Denmark FX We thank Ulla Rasmussen and Karen Broz for technical support. Sven Saupe and Mathieu Paoletti at the Centre National de la Recherche Scientifique Universite de Bordeaux are thanked for scientific discussion. The Minnesota Supercomputing Institute is kindly acknowledged for computing resources and support. This study was supported by The Villum Foundation, Denmark. The authors declare no conflicts of interest. NR 74 TC 0 Z9 0 U1 9 U2 15 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD JUN PY 2016 VL 6 IS 6 BP 1585 EP 1595 DI 10.1534/g3.116.027987 PG 11 WC Genetics & Heredity SC Genetics & Heredity GA DO5KI UT WOS:000377821600010 PM 27172188 ER PT J AU Thyssen, GN Fang, DD Zeng, LH Song, XL Delhom, CD Condon, TL Li, P Kim, HJ AF Thyssen, Gregory N. Fang, David D. Zeng, Linghe Song, Xianliang Delhom, Christopher D. Condon, Tracy L. Li, Ping Kim, Hee Jin TI The Immature Fiber Mutant Phenotype of Cotton (Gossypium hirsutum) Is Linked to a 22-bp Frame-Shift Deletion in a Mitochondria Targeted Pentatricopeptide Repeat Gene SO G3-GENES GENOMES GENETICS LA English DT Article DE Cotton fiber cell wall thickness; fiber maturity; frame-shift deletion; im locus; pentatricopeptide repeat (PPR) ID STRESS RESPONSES; IM MUTANT; RNA-SEQ; L.; PROTEINS; ARABIDOPSIS; POPULATIONS; IDENTIFICATION; ELONGATION; EXPRESSION AB Cotton seed trichomes are the most important source of natural fibers globally. The major fiber thickness properties influence the price of the raw material, and the quality of the finished product. The recessive immature fiber (im) gene reduces the degree of fiber cell wall thickening by a process that was previously shown to involve mitochondrial function in allotetraploid Gossypium hirsutum. Here, we present the fine genetic mapping of the im locus, gene expression analysis of annotated proteins near the locus, and association analysis of the linked markers. Mapping-by-sequencing identified a 22-bp deletion in a pentatricopeptide repeat (PPR) gene that is completely linked to the immature fiber phenotype in 2837 F-2 plants, and is absent from all 163 cultivated varieties tested, although other closely linked marker polymorphisms are prevalent in the diversity panel. This frame-shift mutation results in a transcript with two long open reading frames: one containing the N-terminal transit peptide that targets mitochondria, the other containing only the RNA-binding PPR domains, suggesting that a functional PPR protein cannot be targeted to mitochondria in the im mutant. Taken together, these results suggest that PPR gene Gh_A03G0489 is involved in the cotton fiber wall thickening process, and is a promising candidate gene at the im locus. Our findings expand our understanding of the molecular mechanisms that modulate cotton fiber fineness and maturity, and may facilitate the development of cotton varieties with superior fiber attributes. C1 [Thyssen, Gregory N.; Fang, David D.; Song, Xianliang; Condon, Tracy L.; Li, Ping; Kim, Hee Jin] ARS, Cotton Fiber Biosci Res Unit, USDA, SRRC, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [Thyssen, Gregory N.] ARS, Cotton Chem & Utilizat Unit, USDA, SRRC, New Orleans, LA 70124 USA. [Delhom, Christopher D.] ARS, Cotton Struct & Qual Res Unit, USDA, SRRC, New Orleans, LA 70124 USA. [Zeng, Linghe] ARS, Crop Genet Res Unit, USDA, Stoneville, MS 38776 USA. [Song, Xianliang] Shandong Agr Univ, Coll Agron, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China. RP Kim, HJ (reprint author), ARS, Cotton Fiber Biosci Res Unit, USDA, SRRC, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM heejin.kim@ars.usda.gov FU USDA-ARS CRIS [6054-21000-017-00D]; Cotton Incorporated [12-199] FX We appreciate Mrs. Sheron Simpson and Dr. Brian Scheffler at the Genomics and Bioinformatics Research Unit, USDA-ARS at Stoneville, MS, for their support in SSR and indel marker analysis. We appreciate Dr. Marina Naoumkina of the Cotton Fiber Bioscience Unit, USDA-ARS-SRRC in New Orleans, LA, for assistance with statistical analysis. This project was financially supported by the USDA-ARS CRIS project no. 6054-21000-017-00D and Cotton Incorporated project no. 12-199. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture, which is an equal opportunity provider and employer. NR 40 TC 1 Z9 1 U1 8 U2 8 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD JUN PY 2016 VL 6 IS 6 BP 1627 EP 1633 DI 10.1534/g3.116.027649 PG 7 WC Genetics & Heredity SC Genetics & Heredity GA DO5KI UT WOS:000377821600014 PM 27172184 ER PT J AU Phansak, P Soonsuwon, W Hyten, DL Song, QJ Cregan, PB Graef, GL Specht, JE AF Phansak, Piyaporn Soonsuwon, Watcharin Hyten, David L. Song, Qijian Cregan, Perry B. Graef, George L. Specht, James E. TI Multi-Population Selective Genotyping to Identify Soybean [Glycine max (L.) Merr.] Seed Protein and Oil QTLs SO G3-GENES GENOMES GENETICS LA English DT Article DE germplasm survey tool; QTLs: pleiotropy or linkage; rare alleles; nonunique SNP accessions; selection bias ID QUANTITATIVE TRAIT LOCUS; EXPERIMENTAL-DESIGN; MARKER LOCI; MAPPING QTL; LINKAGE; ASSOCIATION; YIELD; LINES; GRAIN AB Plant breeders continually generate ever-higher yielding cultivars, but also want to improve seed constituent value, which is mainly protein and oil, in soybean [Glycine max (L.) Merr.]. Identification of genetic loci governing those two traits would facilitate that effort. Though genome-wide association offers one such approach, selective genotyping of multiple biparental populations offers a complementary alternative, and was evaluated here, using 48 F-2:3 populations (n=approximate to 224 plants) created by mating 48 high protein germplasm accessions to cultivars of similar maturity, but with normal seed protein content. All F-2:3 progeny were phenotyped for seed protein and oil, but only 22 high and 22 low extreme progeny in each F-2:3 phenotypic distribution were genotyped with a 1536-SNP chip (ca. 450 bimorphic SNPs detected per mating). A significant quantitative trait locus (QTL) on one or more chromosomes was detected for protein in 35 (73%), and for oil in 25 (52%), of the 48 matings, and these QTL exhibited additive effects of 4gkg(-1) and R-2 values of 0.07 or more. These results demonstrated that a multiple-population selective genotyping strategy, when focused on matings between parental phenotype extremes, can be used successfully to identify germplasm accessions possessing large-effect QTL alleles. Such accessions would be of interest to breeders to serve as parental donors of those alleles in cultivar development programs, though 17 of the 48 accessions were not unique in terms of SNP genotype, indicating that diversity among high protein accessions in the germplasm collection is less than what might ordinarily be assumed. C1 [Phansak, Piyaporn; Soonsuwon, Watcharin; Hyten, David L.; Graef, George L.; Specht, James E.] Univ Nebraska, Dept Agron & Hort, 363 Keim Hall,Fair St,East Campus, Lincoln, NE 68583 USA. [Song, Qijian; Cregan, Perry B.] ARS, Soybean Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. [Phansak, Piyaporn] Nakhon Phanom Univ, Div Biol, Fac Sci, Muang 48000, Nakhon Phanom, Thailand. [Soonsuwon, Watcharin] Prince Songkla Univ, Dept Plant Sci, Fac Nat Resources, Hat Yai 90112, Songkhla, Thailand. RP Specht, JE (reprint author), Univ Nebraska, Dept Agron & Hort, 363 Keim Hall,Fair St,East Campus, Lincoln, NE 68583 USA. EM jspecht1@unl.edu FU Committee Staff Development Project on Higher Education, Royal Thai Government; Faculty of Natural Resources, Prince of Songkla University, Thailand; Nebraska Soybean Board; United Soybean Board FX The authors thank Dr. Karl W. Broman (Department of Biostatistics and Medical Informatics, School of Medicine and Public Health, University of Wisconsin, Madison, WI) for providing much assistance and advice in the use of Rqtl for the QTL linkage mapping in this multipopulation SG study. Piyaporn Phansak's Ph.D. program was funded by a scholarship from the Committee Staff Development Project on Higher Education, Royal Thai Government. Watcharin Soonsuwon's Ph.D. program was funded by a scholarship from the Faculty of Natural Resources, Prince of Songkla University, Thailand. Initial funding for the phenotyping phase of this research project was provided by the Nebraska Soybean Board. Subsequent funding for the genotyping phase was provided by the United Soybean Board. NR 50 TC 0 Z9 0 U1 11 U2 16 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD JUN PY 2016 VL 6 IS 6 BP 1635 EP 1648 DI 10.1534/g3.116.027656 PG 14 WC Genetics & Heredity SC Genetics & Heredity GA DO5KI UT WOS:000377821600015 PM 27172185 ER PT J AU Wright, M Lima, I Bigner, R AF Wright, Maureen Lima, Isabel Bigner, Renee TI Microbial and physicochemical properties of sugarcane bagasse for potential conversion to value-added products SO INTERNATIONAL SUGAR JOURNAL LA English DT Article DE sugarcane bagasse; bagasse storage; fuel value ID LIFE-CYCLE ASSESSMENT; PROXIMATE ANALYSIS; ELECTRICITY-GENERATION; CANE BAGASSE; RESIDUES; BIOMASS; ENERGY; PAPER; MANUFACTURE; STRATEGIES AB Sugarcane bagasse is a potential source for commercially-viable products such as animal feed, mulch, or fuel. The applications will be determined by the levels of moisture, ash and beneficial chemicals in the bagasse. Manufacturing value-added products will be impacted by microbes, and may require microbial conversion of the substrate. Microbes present in bagasse have potential for conversion of bagasse to beneficial products because they have the ability to metabolize fibers, and to survive the environmental conditions in bagasse. These microbes also have the potential to interfere with the production of desired products. An analysis of both microbes and chemicals present in bagasse is necessary to determine the most viable potential applications. This study evaluates the microbial and physico-chemical properties in sugarcane bagasse samples from three different varieties in a research setting, and samples of mixed bagasse varieties at three factories. All samples were collected in south Louisiana. Stalks from individual varieties were either shredded or passed through a roller mill. The fresh mixed variety bagasse was collected from the final tandem mill at a factory. The stored mixed variety bagasse was collected from piles at the factories. Samples for microbial analysis were grown on two types of growth media. One medium, MRS, selects for Leuconostoc, Lactobacillus, and other microbes that metabolize sugars and produce degradation products. The second medium, NA, allows growth of a broad range of microbes which are present in the cane growing environment. Microbial counts and types were compared across varieties and processing methods, and were correlated with physicochemical analyses. Fuel value of bagasse was significantly reduced when the bagasse was stored uncovered. C1 [Wright, Maureen; Lima, Isabel; Bigner, Renee] USDA ARS, SRRC, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. RP Wright, M (reprint author), USDA ARS, SRRC, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM Maureen.Wright@ars.usda.gov NR 30 TC 0 Z9 0 U1 4 U2 4 PU INT SUGAR JOURNAL LTD PI KENT PA 80 CALVERLEY, TUNBRIDGE WELLS, KENT TN1 2UN, WALES SN 0020-8841 J9 INT SUGAR J JI Int. Sugar J. PD JUN PY 2016 VL 118 IS 1410 BP 424 EP + PG 9 WC Agronomy; Food Science & Technology SC Agriculture; Food Science & Technology GA DO6WY UT WOS:000377925400031 ER PT J AU Kim, MJ Moon, Y Tou, JC Mou, BQ Waterland, NL AF Kim, Moo Jung Moon, Youyoun Tou, Janet C. Mou, Beiquan Waterland, Nicole L. TI Nutritional value, bioactive compounds and health benefits of lettuce (Lactuca sativa L.) SO JOURNAL OF FOOD COMPOSITION AND ANALYSIS LA English DT Review DE Food composition; Lettuce types; Nutrition; Minerals; Bioactive compounds; Vitamins; Carotenoids; Phenolic compounds ID DIETARY FIBER INTAKE; VITAMIN-C CONTENT; PHENOLIC-COMPOUNDS; LEAF LETTUCE; UNITED-STATES; ANTIOXIDANT PROPERTIES; COOKED VEGETABLES; BLOOD-PRESSURE; BETA-CAROTENE; PLANT FOODS AB Lettuce is one of the most widely consumed vegetables worldwide, but its nutritional value has been underestimated. Lettuce is low in calories, fat and sodium. It is a good source of fiber, iron, folate, and vitamin C. Lettuce is also a good source of various other health-beneficial bioactive compounds. In vitro and in vivo studies have shown anti-inflammatory, cholesterol-lowering, and anti-diabetic activities attributed to the bioactive compounds in lettuce. However, nutrient composition and bioactive compounds vary among lettuce types. Crisphead lettuce, the most popular type of lettuce in the US, is comparatively low in minerals, vitamins, and bioactive compounds. More nutritious lettuces are leaf type lettuce and romaine with folate content comparable to other rich leafy vegetable sources. Red pigmented lettuce contains higher phenolic compounds than green lettuce. Due to increasing popularity, the nutrient contents in baby lettuce were also examined. Baby green romaine was especially high in vitamin C. In this review composition and nutritional value of different lettuce types were summarized, which can help growers and consumers choose lettuce types with higher nutritional benefits. (C) 2016 Elsevier Inc. All rights reserved. C1 [Kim, Moo Jung; Moon, Youyoun; Waterland, Nicole L.] W Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA. [Kim, Moo Jung; Tou, Janet C.] W Virginia Univ, Div Anim & Nutr Sci, Morgantown, WV 26506 USA. [Mou, Beiquan] ARS, USDA, Salinas, CA USA. RP Waterland, NL (reprint author), W Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA. EM Nicole.Waterland@mail.wvu.edu NR 112 TC 0 Z9 0 U1 26 U2 45 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0889-1575 EI 1096-0481 J9 J FOOD COMPOS ANAL JI J. Food Compos. Anal. PD JUN PY 2016 VL 49 BP 19 EP 34 DI 10.1016/j.jfca.2016.03.004 PG 16 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DO5PT UT WOS:000377835900003 ER PT J AU Xiao, ZL Codling, EE Luo, YG Nou, XW Lester, GE Wang, Q AF Xiao, Zhenlei Codling, Eton E. Luo, Yaguang Nou, Xiangwu Lester, Gene E. Wang, Qin TI Microgreens of Brassicaceae: Mineral composition and content of 30 varieties SO JOURNAL OF FOOD COMPOSITION AND ANALYSIS LA English DT Article DE Microgreens; Brassicaceae; Food composition; Macroelements; Microelements; ICP OES; Hierarchical cluster analysis ID LEAFY VEGETABLES; RAW; INCREASES; MATURITY; ELEMENTS; BROCCOLI; PROFILE; L. AB The mineral element composition was analyzed for 30 varieties of microgreens, representing 10 species within 6 genera of the Brassicaceae family. Brassicaceae microgreens were assayed for concentrations of macroelements, including calcium (Ca), magnesium (Mg), phosphorous (P), sodium (Na), potassium (K), and of microelements, including copper (Cu), iron (Fe), manganese (Mn), and zinc (Zn). Determinations of mineral elements in microgreen samples were performed using an inductively coupled plasma optical emission spectrophotometer (ICP OES). Potassium was the most abundant macroelement ranging from 176 to 387 mg/100 g fresh weight (FW), followed by P (52-86 mg/100 g FW), Ca (28-66 mg/100 g FW), Mg (28-66 mg/100 g FW), and Na (19-68 mg/100 g FW). Among the microelements, Fe tended to be most abundant (0.47-0.84 mg/100 g FW), followed by Zn (0.22-0.51 mg/100 g FW), Mn (0.17-0.48 mg/100 g FW), and Cu (0.041-0.13 mg/100 g FW). Based upon the analysis of 30 varieties, the results demonstrate that microgreens are good sources of both macroelements (K and Ca) and microelements (Fe and Zn.). Consumption of microgreens could be a health-promoting strategy to meet dietary reference intake requirements for essential elements beneficial to human health. Published by Elsevier Inc. C1 [Xiao, Zhenlei; Luo, Yaguang; Lester, Gene E.] USDA ARS, Food Qual Lab, Beltsville, MD 20705 USA. [Xiao, Zhenlei; Wang, Qin] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20740 USA. [Codling, Eton E.] USDA ARS, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. [Nou, Xiangwu] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. RP Luo, YG (reprint author), USDA ARS, Food Qual Lab, Beltsville, MD 20705 USA. EM yaguang.luo@ars.usda.gov FU USDA-ARS [8042-43440-005D] FX The authors thank Fresh Origins for planting the microgreens samples for this study. Special thanks to Ms. Mebrat Gesese for her technical assistance throughout the work. Use of a company or product name by the U.S. Department of Agriculture does not imply approval or recommendation of the product to the exclusion of others that may also be suitable. This research was funded by USDA-ARS project no. 8042-43440-005D. NR 22 TC 1 Z9 1 U1 11 U2 18 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0889-1575 EI 1096-0481 J9 J FOOD COMPOS ANAL JI J. Food Compos. Anal. PD JUN PY 2016 VL 49 BP 87 EP 93 DI 10.1016/j.jfca.2016.04.006 PG 7 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DO5PT UT WOS:000377835900010 ER PT J AU Snodgrass, RG Huang, SR Namgaladze, D Jandali, O Shao, T Sama, S Brune, B Hwang, DH AF Snodgrass, Ryan G. Huang, Shurong Namgaladze, Dmitry Jandali, Ola Shao, Tiffany Sama, Spandana Bruene, Bernhard Hwang, Daniel H. TI Docosahexaenoic acid and palmitic acid reciprocally modulate monocyte activation in part through endoplasmic reticulum stress SO JOURNAL OF NUTRITIONAL BIOCHEMISTRY LA English DT Article DE Monocyte; Inflammation; Inflammasome; Fatty acid; ER stress; DHA ID POLYUNSATURATED FATTY-ACIDS; PROTEIN-MISFOLDING DISEASES; TOLL-LIKE RECEPTOR-4; ER STRESS; BETA-CELLS; APOPTOSIS; MACROPHAGES; INFLAMMASOME; INHIBITION; HOMEOSTASIS AB Palmitic acid (C16:0) and TLR2 ligand induce, but docosahexaenoic acid (DHA) inhibits monocyte activation. C16:0 and TLR2 or TLR4 ligand induce certain ER stress markers; thus, we determined whether ER stress induced by these agonists is sufficient to induce monocyte activation, and whether the ER stress is inhibited by DHA which is known to inhibit C16:0- or ligand-induced TLR activation. Monocyte activation and ER stress were assessed by TLR/inflammasome-induced IL-1 beta production, and phosphorylation of IRE-1 and eIF2 and expression of CHOP, respectively in THP-1 cells. TLR2 ligand Pam3C5K4 induced phosphorylation of elF2, but not phosphorylation of IRE-1 and CHOP expression. LPS also induced phosphorylation of both IRE-1 and eIF2 but not CHOP expression suggesting that TLR2 or TLR4 ligand, or C16:0 induces different ER stress responses. C16:0-, Pam3CSK4-, or LPS-induced IL-1 beta production was inhibited by 4-phenylbutyric acid, an inhibitor of ER stress suggesting that IL-1 beta production induced by these agonists is partly mediated through ER stress. Among two ER stress-inducing molecules, thapsigargin but not tunicamycin led to the expression of pro-IL-1 beta and secretion of IL-1 beta. Thus, not all types of ER stress are sufficient to induce inflammasome-mediated IL-1 beta secretion in monocytes. Although both C16:0 and thapsigargin-induced IL-1 beta secretion was inhibited by DHA, only C16:0-mediated ER stress was responsive to DHA. These findings suggest that the anti-inflammatory effects of DHA are at least in part mediated through modulating ER homeostasis and that the propensity of ER stress can be differentially modulated by the types of dietary fat we consume. (C) 2016 Elsevier Inc. All rights reserved. C1 [Snodgrass, Ryan G.; Huang, Shurong; Jandali, Ola; Shao, Tiffany; Sama, Spandana; Hwang, Daniel H.] ARS, USDA, Western Human Nutr Res Ctr, 430 West Hlth Sci Dr, Davis, CA 95616 USA. [Snodgrass, Ryan G.; Namgaladze, Dmitry; Bruene, Bernhard] Goethe Univ Frankfurt, Inst Biochem 1, D-60054 Frankfurt, Germany. RP Hwang, DH (reprint author), ARS, USDA, Western Human Nutr Res Ctr, 430 West Hlth Sci Dr, Davis, CA 95616 USA. EM daniel.hwang@ars.usda.gov FU USDA-ARS program project [5306-51530-017-00D]; Deutsche Forschungsgemeinschaft [SFB 1039]; USDA/NIFA competitive grant [2013-03477] FX This work was supported by USDA-ARS program project (5306-51530-017-00D), Deutsche Forschungsgemeinschaft (SFB 1039, Teilprojekt A05, B04) and USDA/NIFA competitive grant 2013-03477. NR 49 TC 1 Z9 1 U1 1 U2 4 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0955-2863 EI 1873-4847 J9 J NUTR BIOCHEM JI J. Nutr. Biochem. PD JUN PY 2016 VL 32 BP 39 EP 45 DI 10.1016/j.jnutbio.2016.01.010 PG 7 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA DO2TZ UT WOS:000377634300004 PM 27142735 ER PT J AU Kim, K Whelan, G Molina, M Purucker, ST Pachepsky, Y Guber, A Cyterski, MJ Franklin, DH Blaustein, RA AF Kim, Keewook Whelan, Gene Molina, Marirosa Purucker, S. Thomas Pachepsky, Yakov Guber, Andrey Cyterski, Michael J. Franklin, Dorcas H. Blaustein, Ryan A. TI Rainfall-induced release of microbes from manure: model development, parameter estimation, and uncertainty evaluation on small plots SO JOURNAL OF WATER AND HEALTH LA English DT Article DE bootstrap; Bradford-Schijven release model; manure; microbe; PEST; quantitative microbial risk assessment (QMRA) ID ESCHERICHIA-COLI; E. COLI; DIE-OFF; SURVIVAL; ENTEROCOCCI; BACTERIA; CRYPTOSPORIDIUM; TRANSPORT; COWPATS; GIARDIA AB A series of simulated rainfall-runoff experiments with applications of different manure types (cattle solid pats, poultry dry litter, swine slurry) was conducted across four seasons on a field containing 36 plots (0.75 x 2 m each), resulting in 144 rainfall-runoff events. Simulating time-varying release of Escherichia coli, enterococci, and fecal coliforms from manures applied at typical agronomic rates evaluated the efficacy of the Bradford-Schijven model modified by adding terms for release efficiency and transportation loss. Two complementary, parallel approaches were used to calibrate the model and estimate microbial release parameters. The first was a four-step sequential procedure using the inverse model PEST, which provides appropriate initial parameter values. The second utilized a PEST/bootstrap procedure to estimate average parameters across plots, manure age, and microbe, and to provide parameter distributions. The experiment determined that manure age, microbe, and season had no clear relationship to the release curve. Cattle solid pats released microbes at a different, slower rate than did poultry dry litter or swine slurry, which had very similar release patterns. These findings were consistent with other published results for both bench-and field-scale, suggesting the modified Bradford-Schijven model can be applied to microbial release from manure. C1 [Kim, Keewook] US DOE, Oak Ridge Inst Sci & Educ, Oak Ridge, TN 37830 USA. [Kim, Keewook] Univ Idaho, Idaho Falls Ctr Higher Educ, Idaho Falls, ID 83402 USA. [Kim, Keewook; Whelan, Gene; Molina, Marirosa; Purucker, S. Thomas; Cyterski, Michael J.] US EPA, Natl Exposure Res Lab, Ecosyst Res Div, Athens, GA 30605 USA. [Pachepsky, Yakov] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. [Guber, Andrey] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Franklin, Dorcas H.] ARS, USDA, Watkinsville, GA 30677 USA. [Franklin, Dorcas H.] Univ Georgia, Dept Crop & Soil Sci, Athens, GA 30602 USA. [Blaustein, Ryan A.] Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA. RP Kim, K (reprint author), US DOE, Oak Ridge Inst Sci & Educ, Oak Ridge, TN 37830 USA.; Kim, K (reprint author), Univ Idaho, Idaho Falls Ctr Higher Educ, Idaho Falls, ID 83402 USA. EM kkeewook@uidaho.edu FU United States Environmental Protection Agency through its Office of Research and Development; Office of Water; Office of Research and Development FX The United States Environmental Protection Agency through its Office of Research and Development and in collaboration with the Office of Water funded and managed the research described here. It has been subjected to Agency review and approved for publication. This research was supported in part by an appointment to the Research Participation Program at the United States Environmental Protection Agency, Office of Research and Development, administered by the Oak Ridge Institute for Science and Education through Interagency Agreement No. DW8992298301 between the United States Department of Energy and the United States Environmental Protection Agency. The Richard B. Russell Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, in Athens, GA provided technical assistance on the field work. Data collection and collation services were provided by students under contract with the Student Services Contracting Authority. NR 40 TC 0 Z9 0 U1 3 U2 4 PU IWA PUBLISHING PI LONDON PA ALLIANCE HOUSE, 12 CAXTON ST, LONDON SW1H0QS, ENGLAND SN 1477-8920 J9 J WATER HEALTH JI J. Water Health PD JUN PY 2016 VL 14 IS 3 BP 443 EP 459 DI 10.2166/wh.2016.239 PG 17 WC Environmental Sciences; Public, Environmental & Occupational Health; Microbiology; Water Resources SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Microbiology; Water Resources GA DO5HH UT WOS:000377813700008 PM 27280610 ER PT J AU Buntyn, JO Carroll, JA Smith, T Rivera, JD Sanchez, NCB Broadway, PR Falkenberg, SM Schmidt, TB AF Buntyn, J. O. Carroll, J. A. Smith, T. Rivera, J. D. Sanchez, N. C. Burdick Broadway, P. R. Falkenberg, S. M. Schmidt, T. B. TI Evaluation Evaluation of the innate immune response of Angus heifers with genetic marker variation for intramuscular fat deposition following a lipopolysaccharide challenge SO LIVESTOCK SCIENCE LA English DT Article DE Acute phase response; Cattle; Cytokines; Marbling; Quantitative trait loci ID ACUTE-PHASE RESPONSE; PROINFLAMMATORY CYTOKINES; HAPTOGLOBIN; HEMOGLOBIN; FEVER; CATTLE; HEME; METABOLISM; MECHANISMS; NUTRITION AB This study evaluated the effect of genetic selection for markers related to marbling deposition in Angus heifers on the immune response following a lipopolysaccharide (LPS) challenge. Fall-born heifers (n=19; similar to 7 months of age, 274 +/- 24 kg) with genetic variation for marbling were utilized including 10 heifers with no genetic markers (No-MVP) and 9 heifers with >= 1 markers (1 + MVP). Genotypic variation for quantitative trait loci QG1 and QG2 heifers was determined. Twenty-four hour (h) prior to the LPS challenge, heifers were fitted with jugular catheters and vaginal temperature (VT) monitoring devices. Temperature monitoring devises were programed to record VT in 1 min intervals. On day (d) 0, 2 h prior to LPS challenge and 8 h post challenge, serum was collected in 0.5 h intervals (0800-1800 h). Serum was also collected at 12, 16, 20, and 24 h post LPS challenge. The LPS challenge was administered to the heifers at 1000 h via intravenous bolus (0.5 mu g/kg BW). Serum was analyzed for cortisol, TNF-alpha, IL-6, IFN-gamma, and haptoglobin (HAPT). A treatment x time interaction existed (P < 0.001) for VT; prior the LPS challenge, VT was similar. Vaginal temperature was elevated from 6 to 21 h post-LPS in 1 + MVP compared to No-MVP. A treatment x time interaction (P=0.02) was observed for IFN-gamma. Prior to the LPS challenge, IFN-gamma concentrations were similar between the two groups. However, 3.5-7 h post-LPS concentrations of IFN-gamma were greater (P < 0.05) for 1 + MVP compared to No -MVP. There was no treatment effect (P >= 0.17) for cortisol, TNF-alpha or IL-6. A treatment effect (P=0.02) was observed for HAVE; 1 + MVP had decreased RAPT compared to No-MVP. Overall, results suggest that heifers selected based upon genetic markers for marbling may have an altered immune in response when challenged with LPS. (C) 2016 Elsevier B.V. All rights reserved. C1 [Buntyn, J. O.; Schmidt, T. B.] Univ Nebraska, Dept Anim Sci, Lincoln, NE 68583 USA. [Carroll, J. A.; Sanchez, N. C. Burdick; Broadway, P. R.] USDA ARS, Livestock Issues Res Unit, Lubbock, TX 79403 USA. [Smith, T.] Mississippi State Univ, Dept Anim & Dairy Sci, Starkville, MS 39762 USA. [Rivera, J. D.] Mississippi Ag & Forestry Exp Stn, Poplaville, MS 39470 USA. [Falkenberg, S. M.] ARS, USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA. RP Schmidt, TB (reprint author), A213 Anim Sci,POB 830908, Lincoln, NE 68583 USA. EM tschmidt4@unl.edu NR 39 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1871-1413 EI 1878-0490 J9 LIVEST SCI JI Livest. Sci. PD JUN PY 2016 VL 188 BP 103 EP 110 DI 10.1016/j.livsci.2016.04.013 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA DO4CN UT WOS:000377729500014 ER PT J AU Chu, CJ Kleinhesselink, AR Havstad, KM McClaran, MP Peters, DP Vermeire, LT Wei, HY Adler, PB AF Chu, Chengjin Kleinhesselink, Andrew R. Havstad, Kris M. McClaran, Mitchel P. Peters, Debra P. Vermeire, Lance T. Wei, Haiyan Adler, Peter B. TI Direct effects dominate responses to climate perturbations in grassland plant communities SO NATURE COMMUNICATIONS LA English DT Article ID INTEGRAL PROJECTION MODELS; SPECIES INTERACTIONS; GLOBAL CHANGE; COEXISTENCE; DEMOGRAPHY; PRAIRIE; VARIABILITY; COMPETITION; ECOSYSTEMS; MECHANISMS AB Theory predicts that strong indirect effects of environmental change will impact communities when niche differences between competitors are small and variation in the direct effects experienced by competitors is large, but empirical tests are lacking. Here we estimate negative frequency dependence, a proxy for niche differences, and quantify the direct and indirect effects of climate change on each species. Consistent with theory, in four of five communities indirect effects are strongest for species showing weak negative frequency dependence. Indirect effects are also stronger in communities where there is greater variation in direct effects. Overall responses to climate perturbations are driven primarily by direct effects, suggesting that single species models may be adequate for forecasting the impacts of climate change in these communities. C1 [Chu, Chengjin] Sun Yat Sen Univ, SYSU Alberta Joint Lab Biodivers Conservat, State Key Lab Biocontrol & Sch Life Sci, Bldg 408,135 Xingangxi Rd, Guangzhou 510275, Guangdong, Peoples R China. [Chu, Chengjin] Sun Yat Sen Univ, Sch Life Sci, Bldg 408,135 Xingangxi Rd, Guangzhou 510275, Guangdong, Peoples R China. [Chu, Chengjin; Kleinhesselink, Andrew R.; Adler, Peter B.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Chu, Chengjin; Kleinhesselink, Andrew R.; Adler, Peter B.] Utah State Univ, Ctr Ecol, Logan, UT 84322 USA. [Havstad, Kris M.; Peters, Debra P.] New Mexico State Univ, ARS, USDA, Jornada Expt Range & Jornada Basin Long Term Ecol, Las Cruces, NM 88003 USA. [McClaran, Mitchel P.; Wei, Haiyan] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ 85721 USA. [Vermeire, Lance T.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. RP Chu, CJ (reprint author), Sun Yat Sen Univ, SYSU Alberta Joint Lab Biodivers Conservat, State Key Lab Biocontrol & Sch Life Sci, Bldg 408,135 Xingangxi Rd, Guangzhou 510275, Guangdong, Peoples R China.; Chu, CJ (reprint author), Sun Yat Sen Univ, Sch Life Sci, Bldg 408,135 Xingangxi Rd, Guangzhou 510275, Guangdong, Peoples R China. EM chuchjin@mail.sysu.edu.cn FU National Science Foundation [DEB-1054040, DEB-1235828]; Utah Agriculture Experiment station [8881]; National Natural Science Foundation of China [31570426, 31000199]; Program for New Century Excellent Talents in University [NCET-12-0248]; Fundamental Research Funds for the Central Universities [lzujbky-2013-k15]; National Science Foundation; Arizona Experiment Station, USDA-NIFA [ARZT-1392500-M12-197]; USDA-CEAP [AZRT-312860-G-t2-51] FX This research was supported by a National Science Foundation grant to PBA (DEB-1054040) and by the Utah Agriculture Experiment station (journal paper number 8881). C.C. was supported by the National Natural Science Foundation of China (31570426 and 31000199), the Program for New Century Excellent Talents in University (NCET-12-0248) and the Fundamental Research Funds for the Central Universities (lzujbky-2013-k15). A.K. was supported by a Graduate Research Fellowship from the National Science Foundation. M.P.M. was partially supported by the Arizona Experiment Station, USDA-NIFA (ARZT-1392500-M12-197) and USDA-CEAP (AZRT-312860-G-t2-51). This work was funded by the National Science Foundation to New Mexico State University as part of the Jornada Basin LTER (DEB-1235828). NR 36 TC 0 Z9 0 U1 19 U2 30 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD JUN PY 2016 VL 7 AR 11766 DI 10.1038/ncomms11766 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DO6NQ UT WOS:000377900700001 PM 27273085 ER PT J AU Davis, TZ Stegelmeier, BL Lee, ST Collett, MG Green, BT Pfister, JA Evans, TJ Grum, DS Buck, S AF Davis, T. Z. Stegelmeier, B. L. Lee, S. T. Collett, M. G. Green, B. T. Pfister, J. A. Evans, T. J. Grum, D. S. Buck, S. TI White snakeroot poisoning in goats: Variations in toxicity with different plant chemotypes SO RESEARCH IN VETERINARY SCIENCE LA English DT Article DE White snakeroot; Tremetone; Benzofuran ketones; Goats ID GOLDENROD ISOCOMA-PLURIFLORA; RAYLESS GOLDENROD; AGERATINA-ALTISSIMA; TREMETOL; CONSTITUENTS; TOXICOSIS; TREMBLES; KETONES; HORSES AB Tremetone and possibly other benzofuran ketones are believed to be the toxic compounds in white snakeroot. However, disease has not been reproduced with purified toxins and the concentrations of the benzofuran ketones in white snakeroot populations that cause toxicosis have not been documented. The objectives of this study were to compare the toxicity of seven plant populations, better characterize the clinical and pathologic changes of poisoning, and correlate intoxication with benzofuran ketone content. Four of the seven white snakeroot collections were toxic at the dose and duration used in the study. Affected goats became exercise intolerant, had significant serum enzyme changes and histological lesions in the large appendicular muscles. The incidence and severity of poisoning was not correlated with total doses of tremetone or total benzofuran ketone concentrations suggesting they may not be closely involved in producing toxicity and the possible involvement of an unidentified toxin. The results also demonstrate that white snakeroot populations vary chemically and toxicologically. Published by Elsevier Ltd. C1 [Davis, T. Z.; Stegelmeier, B. L.; Lee, S. T.; Green, B. T.; Pfister, J. A.; Grum, D. S.] ARS, USDA, Poisonous Plant Res Lab, 1150 E 1400 N, Logan, UT 84341 USA. [Collett, M. G.] Massey Univ, Inst Vet Anim & Biomed Sci, Private Bag 11222, Palmerston North 4442, New Zealand. [Evans, T. J.] Univ Missouri, Vet Med Diagnost Lab, Columbia, MO USA. [Buck, S.] Univ Illinois, 1816 S Oak St, Champaign, IL USA. RP Davis, TZ (reprint author), ARS, USDA, Poisonous Plant Res Lab, 1150 E 1400 N, Logan, UT 84341 USA. EM zane.davis@ars.usda.gov FU USDA/ARS FX The authors thank Katie Lott, Andrea Dolbear, Chuck Hailes, Joseph Jacobson and Ed Knoppel for their assistance with this research. We would also like to thank Al Parker for his help in identifying locations of plant populations used in this study. This research was supported by USDA/ARS. NR 23 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0034-5288 EI 1532-2661 J9 RES VET SCI JI Res. Vet. Sci. PD JUN PY 2016 VL 106 BP 29 EP 36 DI 10.1016/j.rvsc.2016.02.008 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA DO6WE UT WOS:000377923400005 PM 27234532 ER PT J AU Sparks, JT Dickens, JC AF Sparks, Jackson T. Dickens, Joseph C. TI Bitter-sensitive gustatory receptor neuron responds to chemically diverse insect repellents in the common malaria mosquito Anopheles quadrimaculatus SO SCIENCE OF NATURE LA English DT Article DE Mosquito; Taste; Repellent; Electrophysiology; Chemosensory; Bitter ID AEDES-AEGYPTI; ODORANT RECEPTOR; DEET; GAMBIAE; CULICIDAE; EFFICACY; DIPTERA; ARTHROPODS AB Female mosquitoes feed on blood from animal hosts to obtain nutritional resources used for egg production. These contacts facilitate the spread of harmful human diseases. Chemical repellents are used to disrupt mosquito host-seeking and blood-feeding behaviors; however, little is known about the gustatory sensitivity of mosquitoes to known repellents. Here, we recorded electrical responses from gustatory receptor neurons (GRNs) housed within the labellar sensilla of female Anopheles quadrimaculatus to N, N-diethyl-3-methylbenzamide (DEET), picaridin, IR3535, 2-undecanone, p-menthane-3,8-diol, geraniol, trans-2-hexen-1-ol, quinine, and quinidine. A bitter-sensitive GRN responded to all tested repellents and quinine, a known feeding deterrent. Responses of the bitter-sensitive neuron to quinine and an isomer, quinidine, did not differ. Delayed bursts of electrical activity were observed in response to continuous stimulation with synthetic repellents at high concentrations. Electrophysiological recordings from bitter-sensitive GRNs associated with mosquito gustatory sensilla represent a convenient model to evaluate candidate repellents. C1 [Sparks, Jackson T.; Dickens, Joseph C.] ARS, USDA, Henry A Wallace Beltsville Agr Res Ctr, Invas Insect Biocontrol & Behav Lab, Bldg 007,Rm 030,10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Dickens, JC (reprint author), ARS, USDA, Henry A Wallace Beltsville Agr Res Ctr, Invas Insect Biocontrol & Behav Lab, Bldg 007,Rm 030,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM joseph.dickens@ars.usda.gov FU Deployed War Fighter Protection (DWFP) Research Program - Department of Defense through the Armed Forces Pest Management Board (AFPMB) FX This work was supported in part by a grant to J.C.D. from the Deployed War Fighter Protection (DWFP) Research Program funded by the Department of Defense through the Armed Forces Pest Management Board (AFPMB). The authors are thankful for a critical review provided by Dr. Jonathan D. Bohbot, Department of Entomology, The Hebrew University, Rehovot, Israel. NR 43 TC 0 Z9 0 U1 7 U2 12 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0028-1042 EI 1432-1904 J9 SCI NAT-HEIDELBERG JI Sci. Nat. PD JUN PY 2016 VL 103 IS 5-6 AR 39 DI 10.1007/s00114-016-1367-y PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DO7YP UT WOS:000377999300004 ER PT J AU Benavides, MV Sonstegard, TS Van Tassell, C AF Benavides, Magda Vieira Sonstegard, Tad S. Van Tassell, Curtis TI Genomic Recions Associated with Sheep Resistance to Gastrointestinal Nematodes SO TRENDS IN PARASITOLOGY LA English DT Review ID QUANTITATIVE TRAIT LOCI; OSTERTAGIA-CIRCUMCINCTA INFECTION; FECAL EGG COUNTS; OVINE LYMPHOCYTE ANTIGENS; HAEMONCHUS-CONTORTUS; TRICHOSTRONGYLUS-COLUBRIFORMIS; MERINO SHEEP; GENE-EXPRESSION; NIPPOSTRONGYLUS-BRASILIENSIS; TELADORSAGIA-CIRCUMCINCTA AB Genetic markers for sheep resistance to gastrointestinal parasites have long been sought by the livestock industry as a way to select more resistant individuals and to help farmers reduce parasite transmission by identifying and removing high egg shedders from the flock. Polymorphisms related to the major histocompatibility complex and interferon (IFN)-gamma genes have been the most frequently reported markers associated with infection. Recently, a new picture is emerging from genome-wide studies, showing that not only immune mechanisms are important determinants of host resistance but that gastrointestinal mucus production and hemostasis pathways may also play a role. C1 [Benavides, Magda Vieira] EMBRAPA, Brasilia, DF, Brazil. [Sonstegard, Tad S.] Recombinetics, St Paul, MN USA. [Van Tassell, Curtis] ARS, Anim Genom & Improvement Lab, USDA, Beltsville Agr Res Ctr, Beltsville, MD USA. RP Benavides, MV (reprint author), EMBRAPA, Brasilia, DF, Brazil. EM magda.benavides@embrapa.br NR 94 TC 0 Z9 0 U1 3 U2 5 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1471-4922 EI 1471-5007 J9 TRENDS PARASITOL JI Trends Parasitol. PD JUN PY 2016 VL 32 IS 6 BP 470 EP 480 DI 10.1016/j.pt.2016.03.007 PG 11 WC Parasitology SC Parasitology GA DO4CT UT WOS:000377730100008 PM 27183838 ER PT J AU Liu, CG Shang, QQ Jia, PY Dai, Y Zhou, YH Liu, ZS AF Liu, Chengguo Shang, Qjanqian Jia, Puyou Dai, Yan Zhou, Yonghong Liu, Zengshe TI Tung Oil-Based Unsaturated Co-ester Macromonomer for Thermosetting Polymers: Synergetic Synthesis and Copolymerization with Styrene SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Tung oil; Unsaturated coester; Thermosetting polymer; Unsaturated polyester resin (UPR); Synergetic modification; Azeotropic copolymerization; Curing behavior ID ONE-STEP ACRYLATION; SOYBEAN-OIL; CASTOR-OIL; MECHANICAL-PROPERTIES; RENEWABLE RESOURCES; POLYESTER RESIN; POLYMERIZATION; NANOCOMPOSITES; PERFORMANCE; COMPOSITES AB A novel unsaturated co-ester (co-UE) macro monomer containing both maleates and acrylates was synthesized from tung oil (TO) and its chemical structure was characterized by FT-IR, H-1 NMR, C-13 NMR, and gel permeation chromatography (GPC). The monomer was synthesized via a new synergetic modification of TO, by introducing maleic groups first and acrylic groups subsequently onto TO molecules. The influence of experimental factors on thermomechanical properties of the . cured bioresins was evaluated to better understand structure property relationships of the biomaterials and optimize experimental conditions. The obtained TO-based co-UE monomer possessed a highly polymerizable C=C functionality, consequently resulting in rigid bioplastics with high cross-link densities (v(e)) and excellent mechanical properties. For instance, the bioplastic prepared under the optimal synthesis conditions demonstrated a v(e) of 4.03 X 10(3) mol/m(3), storage modulus at 25 degrees C of 2.40 GPa, and glass transition temperature (T-g) of 127 degrees C, as well as tensile strength and modulus at 36.3 MPa and 1.70 GPa, respectively. A new theory for determining optimal comonomer concentration was further developed according to the copolymerization equation. The proposed theory accurately predicted the best styrene dosage for the co-UE monomer. At last, the hydroxyethyl acrylate (HEA)-modified TO-based resin was compared with the unmodified one in thermomechanical properties, thermal stability, microstructural morphologies, and curing behaviors. The new co-UE bioresin showed higher C=C functionality and cross-link density, superior properties including T-g and thermal stability, and similar curing behaviors. The developed eco-friendly rigid biomaterials provide potential application in structural plastics such as sheet molding compounds. C1 [Liu, Chengguo; Shang, Qjanqian; Jia, Puyou; Dai, Yan; Zhou, Yonghong] Chinese Acad Forestry, Inst Chem Ind Forest Prod, 16 Suojin North Rd, Nanjing 210042, Jiangsu, Peoples R China. [Liu, Chengguo; Shang, Qjanqian; Jia, Puyou; Dai, Yan; Zhou, Yonghong] Natl Engn Lab Biomass Chem Utilizat, 16 Suojin North Rd, Nanjing 210042, Jiangsu, Peoples R China. [Liu, Chengguo; Shang, Qjanqian; Jia, Puyou; Dai, Yan; Zhou, Yonghong] State Forestry Adm, Key Lab Forest Chem Engn, 16 Suojin North Rd, Nanjing 210042, Jiangsu, Peoples R China. [Liu, Chengguo; Shang, Qjanqian; Jia, Puyou; Dai, Yan; Zhou, Yonghong] Key Lab Biomass Energy & Mat, 16 Suojin North Rd, Nanjing 210042, Jiangsu, Peoples R China. [Liu, Zengshe] USDA ARS, Biooils Res, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. RP Zhou, YH (reprint author), Chinese Acad Forestry, Inst Chem Ind Forest Prod, 16 Suojin North Rd, Nanjing 210042, Jiangsu, Peoples R China.; Zhou, YH (reprint author), Natl Engn Lab Biomass Chem Utilizat, 16 Suojin North Rd, Nanjing 210042, Jiangsu, Peoples R China.; Zhou, YH (reprint author), State Forestry Adm, Key Lab Forest Chem Engn, 16 Suojin North Rd, Nanjing 210042, Jiangsu, Peoples R China.; Zhou, YH (reprint author), Key Lab Biomass Energy & Mat, 16 Suojin North Rd, Nanjing 210042, Jiangsu, Peoples R China.; Liu, ZS (reprint author), USDA ARS, Biooils Res, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM yhzhou777@sina.com; kevin.liu@ars.usda.gov FU National Natural Science Foundation of China [31300489]; Fundamental Research Funds for the Central Nonprofit Research Institution of the Chinese Academy of Forestry (CAF) [CAFYBB2014QB022]; Fundamental Research Funds from Jiangsu Province Biomass Energy and Materials Laboratory [JSBEM-S-201501] FX The authors are grateful to the financial support from the National Natural Science Foundation of China (31300489), the Fundamental Research Funds for the Central Nonprofit Research Institution of the Chinese Academy of Forestry (CAF (CAFYBB2014QB022), and the Fundamental Research Funds from Jiangsu Province Biomass Energy and Materials Laboratory (JSBEM-S-201501). NR 43 TC 2 Z9 2 U1 14 U2 27 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD JUN PY 2016 VL 4 IS 6 BP 3437 EP 3449 DI 10.1021/acssuschemeng.6b00466 PG 13 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA DN9UJ UT WOS:000377425900058 ER PT J AU Xie, JL Hse, CY Li, CJ Shupe, TF Hu, TX Qi, JQ De Hoop, CF AF Xie, Jiulong Hse, Chung-Yun Li, Chunjie Shupe, Todd F. Hu, Tingxing Qi, Jinqiu De Hoop, Cornelis F. TI Characterization of Microwave Liquefied Bamboo Potential Use in the Generation of Nanofibrillated Residue and Its Cellulosic Fiber SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Bamboo; Microwave liquefaction; Residue; Nanofibrillated cellulosic fiber; Characterization ID ASSISTED LIQUEFACTION; WOOD LIQUEFACTION; CORN STOVER; FRACTIONATION; LIGNIN; LIGNOCELLULOSE; PRETREATMENT; BIOPOLYOLS; CHEMICALS; POLYOLS AB Bamboo raw feedstocks with large particle size (20-80 mesh) were subjected to a microwave liquefaction system, and the liquefied products were separated into biopolyols and liquefied residues. Biopolyols were first analyzed by gas chromatography mass spectrometry (GC MS), and the main components were sugar derivatives with 2-4 hydroxyl groups and phenolic compounds derived from lignin. The residues were collected and evaluated for potential use in the production of nanofibrillated cellulosic fibers. Results show that liquefied residue content as well as its physicochemical properties varied with respect to particle size, liquefaction temperature, and reaction time. It was also found that residues from liquefaction reaction with the minimum residue content in this study still exhibited traced fiber structure with remaining cellulose attached with recondensed lignin. Pure white cellulose fibers were extracted from the residues with yield of 65.61% using a combination of bleaching and acid hydrolysis treatment. Nanofibrillated cellulosic fibers were generated by given the purified cellulose fibers to high-intensity ultrasonic treatment. The resulted nanofibrillated cellulosic fibers had a range of 4-18 nm in diameter and length of 550 nm or longer, indicating the nanofibers obtained from liquefied bamboo residues hold great potential in reinforcing polymeric matrix materials. The successful isolation of nanofibrillated cellulosic fibers from liquefied residues offers a novel approach to make full use of the liquefied bamboo for value-added green products. C1 [Xie, Jiulong; Hu, Tingxing; Qi, Jinqiu] Sichuan Agr Univ, Coll Forestry, Chengdu 611130, Sichuan, Peoples R China. [Hse, Chung-Yun] US Forest Serv, Southern Res Stn, USDA, Pineville, LA 71360 USA. [Xie, Jiulong; Li, Chunjie; Shupe, Todd F.; De Hoop, Cornelis F.] Louisiana State Univ, Ctr Agr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. RP Hu, TX (reprint author), Sichuan Agr Univ, Coll Forestry, Chengdu 611130, Sichuan, Peoples R China. EM tingxing_hu@163.com FU Key Laboratory of Wood Industry and Furniture Engineering of Sichuan Provincial Colleges and Universities FX This work has been supported by "Key Laboratory of Wood Industry and Furniture Engineering of Sichuan Provincial Colleges and Universities". NR 33 TC 1 Z9 1 U1 12 U2 18 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD JUN PY 2016 VL 4 IS 6 BP 3477 EP 3485 DI 10.1021/acssuschemeng.6600497 PG 9 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA DN9UJ UT WOS:000377425900061 ER PT J AU Zhou, Y Utsunomiya, YT Xu, LY Hay, EA Bickhart, DM Alexandre, PA Rosen, BD Schroeder, SG Carvalheiro, R Neves, HHD Sonstegard, TS Van Tassell, CP Ferraz, JBS Fukumasu, H Garcia, JF Liu, GE AF Zhou, Yang Utsunomiya, Yuri T. Xu, Lingyang Hay, El Hamidi Abdel Bickhart, Derek M. Alexandre, Pamela Almeida Rosen, Benjamin D. Schroeder, Steven G. Carvalheiro, Roberto de Rezende Neves, Haroldo Henrique Sonstegard, Tad S. Van Tassell, Curtis P. Sterman Ferraz, Jose Bento Fukumasu, Heidge Garcia, Jose Fernando Liu, George E. TI Genome-wide CNV analysis reveals variants associated with growth traits in Bos indicus SO BMC GENOMICS LA English DT Article DE Copy number variation (CNV); EBV; Association; Body size; Bos indicus ID COPY NUMBER VARIATION; HOLSTEIN CATTLE; EXPRESSION; CHANNEL; WEIGHT; ANGUS AB Background: Apart from single nucleotide polymorphism (SNP), copy number variation (CNV) is another important type of genetic variation, which may affect growth traits and play key roles for the production of beef cattle. To date, no genome-wide association study (GWAS) for CNV and body traits in beef cattle has been reported, so the present study aimed to investigate this type of association in one of the most important cattle subspecies: Bos indicus (Nellore breed). Results: We have used intensity data from over 700,000 SNP probes across the bovine genome to detect common CNVs in a sample of 2230 Nellore cattle, and performed GWAS between the detected CNVs and nine growth traits. After filtering for frequency and length, a total of 231 CNVs ranging from 894 bp to 4,855,088 bp were kept and tested as predictors for each growth trait using linear regression analysis with principal components correction. There were 49 significant associations identified among 17 CNVs and seven body traits after false discovery rate correction (P < 0.05). Among the 17 CNVs, three were significant or marginally significant for all the traits. We have compared the locations of associated CNVs with quantitative trait locus and the RefGene database, and found two sets of 9 CNVs overlapping with either known QTLs or genes, respectively. The gene overlapping with CNV100, KCNJ12, is a functional candidate for muscle development and plays critical roles in muscling traits. Conclusion: This study presents the first CNV-based GWAS of growth traits using high density SNP microarray data in cattle. We detected 17 CNVs significantly associated with seven growth traits and one of them (CNV100) may be involved in growth traits through KCNJ12. C1 [Zhou, Yang; Xu, Lingyang; Hay, El Hamidi Abdel; Bickhart, Derek M.; Rosen, Benjamin D.; Schroeder, Steven G.; Sonstegard, Tad S.; Van Tassell, Curtis P.; Liu, George E.] USDA ARS, BARC, Anim Genom & Improvement Lab, Room 111,BARC East, Beltsville, MD 20705 USA. [Zhou, Yang] Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Agr Mol Biol, Yangling 712100, Shaanxi, Peoples R China. [Utsunomiya, Yuri T.; Garcia, Jose Fernando] UNESP Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Med Vet Prevent & Reprod Anim, BR-14884900 Jaboticabal, SP, Brazil. [Xu, Lingyang] Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China. [Alexandre, Pamela Almeida; Sterman Ferraz, Jose Bento; Fukumasu, Heidge] Univ Sao Paulo, Coll Anim Sci & Food Engn, Dept Vet Med, BR-13635 Pirassununga, SP, Brazil. [de Rezende Neves, Haroldo Henrique] UNESP Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Zootecnia, BR-14884900 Jaboticabal, SP, Brazil. [Garcia, Jose Fernando] UNESP Univ Estadual Paulista, Fac Med Vet Aracatuba, Dept Apoio Prod & Saude Anim, BR-16050680 Aracatuba, SP, Brazil. [Sonstegard, Tad S.] Recombinet Inc, St Paul, MN 55104 USA. [Garcia, Jose Fernando] IAEA, Collaborating Ctr Anim Genom & Bioinformat, Aracatuba, SP, Brazil. RP Liu, GE (reprint author), USDA ARS, BARC, Anim Genom & Improvement Lab, Room 111,BARC East, Beltsville, MD 20705 USA.; Garcia, JF (reprint author), UNESP Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Med Vet Prevent & Reprod Anim, BR-14884900 Jaboticabal, SP, Brazil.; Garcia, JF (reprint author), UNESP Univ Estadual Paulista, Fac Med Vet Aracatuba, Dept Apoio Prod & Saude Anim, BR-16050680 Aracatuba, SP, Brazil.; Garcia, JF (reprint author), IAEA, Collaborating Ctr Anim Genom & Bioinformat, Aracatuba, SP, Brazil. EM jfgarcia@fmva.unesp.br; george.liu@ars.usda.gov RI Fukumasu, Heidge/B-3866-2013; Utsunomiya, Yuri Tani/K-5214-2013; OI Utsunomiya, Yuri Tani/0000-0002-6526-8337; Schroeder, Steven/0000-0001-9103-5150; Bickhart, Derek/0000-0003-2223-9285 FU Agriculture and Food Research Initiative (AFRI) competitive grant from the USDA National Institute of Food and Agriculture (NIFA) Animal Genome Program [2011-67015-30183] FX We thank Reuben Anderson, and Alexandre Dimtchev for technical assistance. This work was supported in part by Agriculture and Food Research Initiative (AFRI) competitive grant No. 2011-67015-30183 from the USDA National Institute of Food and Agriculture (NIFA) Animal Genome Program. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 30 TC 0 Z9 0 U1 2 U2 8 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUN 1 PY 2016 VL 17 AR 419 DI 10.1186/s12864-016-2461-4 PG 9 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DO1TR UT WOS:000377562500001 PM 27245577 ER PT J AU Souza, CK Rajao, DS Loving, CL Gauger, PC Perez, DR Vincent, AL AF Souza, Carine K. Rajao, Daniela S. Loving, Crystal L. Gauger, Phillip C. Perez, Daniel R. Vincent, Amy L. TI Age at Vaccination and Timing of Infection Do Not Alter Vaccine-Associated Enhanced Respiratory Disease in Influenza A Virus-Infected Pigs SO CLINICAL AND VACCINE IMMUNOLOGY LA English DT Article ID HETEROLOGOUS CHALLENGE; IMMUNE-RESPONSES; CROSS-PROTECTION; UNITED-STATES; SWINE; HUMANS; TRANSMISSION; REPLICATION; H1N2 AB Whole inactivated virus (WIV) vaccines are widely used in the swine industry to reduce clinical disease against homologous influenza A virus (IAV) infection. In pigs experimentally challenged with antigenically distinct heterologous IAV of the same hemagglutinin subtype, WIV vaccinates have been shown to develop vaccine-associated enhanced respiratory disease (VAERD). We evaluated the impact of vaccine valency, age at vaccination, and duration between vaccination and challenge on the development of VAERD using vaccine containing delta 1-H1N2 and challenge with pandemic H1N1 (pH1N1) virus. Pigs were vaccinated with monovalent WIV MN08 (delta 1-H1N2) and bivalent (delta 1-H1N2-H3N2 or delta 1-H1N2-pH1N1) vaccines and then were challenged with pH1N1 at 3 weeks postboost (wpb). Another group was vaccinated with the same monovalent WIV and challenged at 6 wpb to determine if the time postvaccination plays a role in the development of VAERD. In a follow-up study, the impact of age of first WIV vaccination (at 4 versus 9 weeks of age) with a boost 3 weeks later (at 7 versus 12 weeks of age) was evaluated. A monovalent live-attenuated influenza virus (LAIV) vaccine administered at 4 and 7 weeks of age was also included. All mismatched WIV groups had significantly higher lung lesions than the LAIV, bivalent MN08-CA09, and control groups. Age of first vaccination or length of time between booster dose and subsequent challenge did not alter the development of VAERD in WIV-vaccinated pigs. Importantly, the mismatched component of the bivalent MN08-CA09 WIV did not override the protective effect of the matched vaccine component. C1 [Souza, Carine K.; Rajao, Daniela S.; Loving, Crystal L.; Vincent, Amy L.] USDA ARS, Virus & Prion Dis Res Unit, Ames, IA USA. [Souza, Carine K.] Univ Fed Rio Grande do Sul, Virol Lab, Porto Alegre, RS, Brazil. [Gauger, Phillip C.] Iowa State Univ, Ames, IA USA. [Perez, Daniel R.] Univ Georgia, Poultry Diagnost & Res Ctr, Athens, GA 30602 USA. RP Vincent, AL (reprint author), USDA ARS, Virus & Prion Dis Res Unit, Ames, IA USA. EM amy.vincent@ars.usda.gov OI Perez, Daniel/0000-0002-6569-5689; de Souza Rajao, Daniela/0000-0002-0772-0065 FU Capes-Brazil; USDA-ARS FX C.K.S. was a Capes-Brazil fellowship recipient of a Ph.D. sandwich scholarship through the Science without Borders Program. D.S.R. was a CNPq-Brazil fellowship recipient. Funding was provided by USDA-ARS. The USDA is an equal opportunity provider and employer. NR 46 TC 0 Z9 0 U1 3 U2 4 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 1556-6811 EI 1556-679X J9 CLIN VACCINE IMMUNOL JI Clin. Vaccine Immunol. PD JUN PY 2016 VL 23 IS 6 BP 470 EP 482 DI 10.1128/CVI.00563-15 PG 13 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA DO0GO UT WOS:000377457600005 PM 27030585 ER PT J AU Highland, MA Schneider, DA White, SN Madsen-Bouterse, SA Knowles, DP Davis, WC AF Highland, Margaret A. Schneider, David A. White, Stephen N. Madsen-Bouterse, Sally A. Knowles, Donald P. Davis, William C. TI Differences in leukocyte differentiation molecule abundances on domestic sheep (Ovis aries) and bighorn sheep (Ovis canadensis) neutrophils identified by flow cytometry SO COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES LA English DT Article DE Bighorn sheep; Domestic sheep; Flow cytometry; Monoclonal antibody; Leukocyte; Autofluorescence; Eosinophil; Neutrophil; Leukocyte differentiation molecule; Major histocompatibility complex ID PASTEURELLA-HAEMOLYTICA LEUKOTOXIN; GAMMA-DELTA T; MONOCLONAL-ANTIBODIES; CD ANTIGENS; WORKSHOP; CELLS; EXPRESSION; REACTIVITY; CATTLE; AUTOFLUORESCENCE AB Although both domestic sheep (DS) and bighorn sheep (BHS) are affected by similar respiratory bacterial pathogens, experimental and field data indicate BHS are more susceptible to pneumonia. Cross-reactive monoclonal antibodies (mAbs) for use in flow cytometry (FC) are valuable reagents for interspecies comparative immune system analyses. This study describes cross-reactive mAbs that recognize leukocyte differentiation molecules (LDMs) and major histocompatibility complex antigens on DS and BHS leukocytes. Characterization of multichannel eosinophil autofluorescence in this study permitted cell-type specific gating of granulocytes for evaluating LDMs, specifically on neutrophils, by single-label FC. Evaluation of relative abundances of LDMs by flow cytometry revealed greater CD11a, CD11b, CD18 (beta(2) integrins) and CD 172a (SIRP alpha) on DS neutrophils and greater CD14 (lipopolysaccharide receptor) on BHS neutrophils. Greater CD25 (IL-2) was identified on BHS lymphocytes following Concavalin A stimulation. While DS and BHS have similar total peripheral blood leukocyte counts, BHS have proportionately more neutrophils. Published by Elsevier Ltd. C1 [Highland, Margaret A.; Schneider, David A.; White, Stephen N.; Madsen-Bouterse, Sally A.; Knowles, Donald P.; Davis, William C.] Washington State Univ, Coll Vet Med, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Highland, Margaret A.; Schneider, David A.; White, Stephen N.; Knowles, Donald P.] USDA, Anim Dis Res Unit, Pullman, WA 99164 USA. RP Highland, MA (reprint author), Washington State Univ, USDA, Anim Dis Res Unit, 3033 ADBF, Pullman, WA 99164 USA. EM Maggie.Highland@ARS.USDA.GOV; David.Schneider1@ARS.USDA.GOV; Stephen.White@ARS.USDA.GOV; smadsen@vetmed.wsu.edu; Don.Knowles@ARS.USDA.GOV; davisw@vetmed.wsu.edu OI Schneider, David/0000-0001-9659-6731 FU Zoetis (previously Pfizer) Animal Helath-Morris Animal Foundation [145-02-13K-2540-0992]; ADRU-ARS-USDA CRIS Project funding [2090-32000-031-00D] FX We would like to thank Dr. Siddra A. Hines, Dr. Rohanna Dassanayake, Victoria Mack, and James Reynolds for advice and technical assistance and Dr. Charles Frevert for technical editing and critical review of the manuscript. This work was supported by a fellowship training grant from Zoetis (previously Pfizer) Animal Helath-Morris Animal Foundation (145-02-13K-2540-0992) and ADRU-ARS-USDA CRIS Project funding ((2090-32000-031-00D). NR 40 TC 0 Z9 0 U1 6 U2 10 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0147-9571 EI 1878-1667 J9 COMP IMMUNOL MICROB JI Comp. Immunol. Microbiol. Infect. Dis. PD JUN PY 2016 VL 46 BP 40 EP 46 DI 10.1016/j.cimid.2016.04.006 PG 7 WC Immunology; Microbiology; Veterinary Sciences SC Immunology; Microbiology; Veterinary Sciences GA DN7YU UT WOS:000377296900007 PM 27260809 ER PT J AU Li, GH Weng, X Du, XC Wang, XP Feng, HL AF Li, Guanghui Weng, Xiang Du, Xiaocheng Wang, Xiping Feng, Hailin TI Stress wave velocity patterns in the longitudinal-radial plane of trees for defect diagnosis SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE Defect; Inspection; Nondestructive testing; Stress wave velocity; Urban trees ID DECAY AB Acoustic tomography for urban tree inspection typically uses stress wave data to reconstruct tomographic images for the trunk cross section using interpolation algorithm. This traditional technique does not take into account the stress wave velocity patterns along tree height. In this study, we proposed an analytical model for the wave velocity in the longitudinal-radial (LR) plane of a live tree. Both field and laboratory stress wave testing were conducted to determine the stress wave velocity patterns in healthy and defective trees. The results showed that the ratio of the wave velocity at a propagation path angle theta (with respect to the radial direction) to the radial velocity in healthy trees approximated a second-order parabolic curve with respect to the symmetric axis (theta = 0). Our analysis of the velocity patterns indicated that the measured velocities in healthy trees were in a good agreement with the theoretical models. The results of this preliminary study indicated that the stress wave velocity patterns can be used to diagnosis internal defects in urban trees and improve the accuracy of 3D tomographic images in tree inspection applications. (c) 2016 Elsevier B.V. All rights reserved. C1 [Li, Guanghui] Jiangnan Univ, Sch Internet Things IoT Engn, Wuxi, Peoples R China. [Li, Guanghui] Jiangnan Univ, Engn Res Ctr IoT Technol Applicat, Minist Educ, Wuxi, Peoples R China. [Weng, Xiang; Du, Xiaocheng; Feng, Hailin] Zhejiang A&F Univ, Sch Informat Engn, Hangzhou, Zhejiang, Peoples R China. [Wang, Xiping] US Forest Serv, USDA, Forest Prod Lab, Madison, WI USA. RP Li, GH (reprint author), Jiangnan Univ, Sch Internet Things IoT Engn, Wuxi, Peoples R China. EM ghli@jiangnan.edu.cn FU National Natural Science Foundation of China [61472368, 61302185, 61272313]; Zhejiang Provincial International Science and Technology Cooperation Project [2013C24026] FX This research was made possible by the collaboration between the USDA Forest Service, Forest Products Laboratory and Zhejiang Agriculture and Forestry University in China. This study was supported in part by the National Natural Science Foundation of China (Grant No. 61472368, 61302185, 61272313) and Zhejiang Provincial International Science and Technology Cooperation Project (Grant No. 2013C24026). NR 19 TC 0 Z9 0 U1 7 U2 7 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 EI 1872-7107 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD JUN PY 2016 VL 124 BP 23 EP 28 DI 10.1016/j.compag.2016.03.021 PG 6 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA DN8HM UT WOS:000377319800003 ER PT J AU Martin, CK Bhapkar, M Pittas, AG Pieper, CF Das, SK Williamson, DA Scott, T Redman, LM Stein, R Gilhooly, CH Stewart, T Robinson, L Roberts, SB AF Martin, Corby K. Bhapkar, Manju Pittas, Anastassios G. Pieper, Carl F. Das, Sai Krupa Williamson, Donald A. Scott, Tammy Redman, Leanne M. Stein, Richard Gilhooly, Cheryl H. Stewart, Tiffany Robinson, Lisa Roberts, Susan B. CA Comprehensive Assessment Long-term TI Effect of Calorie Restriction on Mood, Quality of Life, Sleep, and Sexual Function in Healthy Nonobese Adults The CALERIE 2 Randomized Clinical Trial SO JAMA INTERNAL MEDICINE LA English DT Article ID LONGITUDINAL DATA-ANALYSIS; URINARY-TRACT SYMPTOMS; LOW-ENERGY DIET; RHESUS-MONKEYS; WEIGHT-LOSS; COMPREHENSIVE ASSESSMENT; REDUCING INTAKE; OBESE MEN; WOMEN; INTERVENTIONS AB IMPORTANCE Calorie restriction (CR) increases longevity in many species and reduces risk factors for chronic diseases. In humans, CR may improve health span, yet concerns remain about potential negative effects of CR. OBJECTIVE To test the effect of CR on mood, quality of life (QOL), sleep, and sexual function in healthy nonobese adults. DESIGN, SETTING, AND PARTICIPANTS A multisite randomized clinical trial (Comprehensive Assessment of Long-term Effects of Reducing Intake of Energy Phase 2 [CALERIE 2]) was conducted at 3 academic research institutions. Adult men and women (N = 220) with body mass index (BMI; calculated as weight in kilograms divided by height in meters squared) of 22.0 to 28.0 were randomized to 2 years of 25% CR or an ad libitum (AL) control group in a 2: 1 ratio favoring CR. Data were collected at baseline, 12 months, and 24 months and examined using intent-to-treat analysis. The study was conducted from January 22, 2007, to March 6, 2012. Data analysis was performed from July 18, 2012, to October 27, 2015. INTERVENTIONS Two years of 25% CR or AL. MAIN OUTCOMES AND MEASURES Self-report questionnaires were administered to measure mood (Beck Depression Inventory-II [BDI-II], score range 0-63, higher scores indicating worse mood, and Profile of Mood States [POMS], with a total mood disturbance score range of -32 to 200 and higher scores indicating higher levels of the constructs measured), QOL (Rand 36-Item Short Form, score range 0-100, higher scores reflecting better QOL, and Perceived Stress Scale, score range 0-40, higher scores indicating higher levels of stress), sleep (Pittsburgh Sleep Quality Index [PSQI], total score range 0-21, higher scores reflecting worse sleep quality), and sexual function (Derogatis Interview for Sexual Function-Self-report, total score range 24-188, higher scores indicating better sexual functioning). RESULTS In all, 218 participants (152 women [69.7%]; mean [SD] age, 37.9 (7.2) years; mean [SD] BMI, 25.1 [1.6]) were included in the analyses. The CR and AL groups lost a mean (SE) of 7.6 (0.3) kg and 0.4 (0.5) kg, respectively, at month 24 (P < .001). Compared with the AL group, the CR group had significantly improved mood (BDI-II: between-group difference [BGD], -0.76; 95% CI, -1.41 to -0.11; effect size [ES], -0.35), reduced tension (POMS: BGD, -0.79; 95% CI, -1.38 to -0.19; ES, -0.39), and improved general health (BGD, 6.45; 95% CI, 3.93 to 8.98; ES, 0.75) and sexual drive and relationship (BGD, 1.06; 95% CI, 0.11 to 2.01; ES, 0.35) at month 24 as well as improved sleep duration at month 12 (BGD, -0.26; 95% CI, -0.49 to -0.02; ES, -0.32) (all P < .05). Greater percent weight loss in the CR group at month 24 was associated with increased vigor (Spearman correlation coefficient, rho = -0.30) and less mood disturbance (rho = 0.27) measured with the POMS, improved general health (rho = -0.27) measured with the SF-36, and better sleep quality per the PSQI total score (rho = 0.28) (all P < .01). CONCLUSIONS AND RELEVANCE In nonobese adults, CR had some positive effects and no negative effects on health-related QOL. C1 [Martin, Corby K.; Williamson, Donald A.; Redman, Leanne M.; Stewart, Tiffany] Louisiana State Univ, Pennington Biomed Res Ctr, 6400 Perkins Rd, Baton Rouge, LA 70808 USA. [Bhapkar, Manju; Pieper, Carl F.] Duke Clin Res Inst, Durham, NC USA. [Bhapkar, Manju; Pieper, Carl F.] Duke Univ, Sch Med, Durham, NC USA. [Pittas, Anastassios G.] Tufts Univ, Sackler Sch Grad Biomed Sci, Boston, MA 02111 USA. [Das, Sai Krupa; Scott, Tammy; Gilhooly, Cheryl H.; Robinson, Lisa; Roberts, Susan B.] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer US Dept Agr, Boston, MA 02111 USA. [Stein, Richard] Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA. RP Martin, CK (reprint author), Louisiana State Univ, Pennington Biomed Res Ctr, 6400 Perkins Rd, Baton Rouge, LA 70808 USA. EM corby.martin@pbrc.edu FU National Institute on Aging; National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health [U01AG022132, U01AG020478, U01AG020487, U01AG020480]; National Obesity Research Center [P30 DK072476]; National Institute of Diabetes and Digestive and Kidney Diseases; National Institute of General Medical Sciences of the National Institutes of Health [1 U54 GM104940] FX The research reported herein was supported by the National Institute on Aging and the National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health (grants U01AG022132, U01AG020478, U01AG020487, and U01AG020480); National Obesity Research Center (grant P30 DK072476), sponsored by the National Institute of Diabetes and Digestive and Kidney Diseases; and the National Institute of General Medical Sciences of the National Institutes of Health, which funds the Louisiana Clinical and Translational Science Center (grant 1 U54 GM104940). NR 32 TC 2 Z9 2 U1 6 U2 13 PU AMER MEDICAL ASSOC PI CHICAGO PA 330 N WABASH AVE, STE 39300, CHICAGO, IL 60611-5885 USA SN 2168-6106 EI 2168-6114 J9 JAMA INTERN MED JI JAMA Intern. Med. PD JUN PY 2016 VL 176 IS 6 BP 743 EP 752 DI 10.1001/jamainternmed.2016.1189 PG 10 WC Medicine, General & Internal SC General & Internal Medicine GA DN6KU UT WOS:000377184000008 PM 27136347 ER PT J AU Bischoff-Ferrari, HA Orav, EJ Dawson-Hughes, B AF Bischoff-Ferrari, Heike A. Orav, E. John Dawson-Hughes, Bess TI Estimating Vitamin D Status and the Choice of Supplementation Dose Reply SO JAMA INTERNAL MEDICINE LA English DT Letter C1 [Bischoff-Ferrari, Heike A.] Univ Zurich Hosp, Dept Geriatr & Aging Res, Raemistr 100, CH-8091 Zurich, Switzerland. [Bischoff-Ferrari, Heike A.] Univ Zurich, Ctr Aging & Mobil, CH-8006 Zurich, Switzerland. [Orav, E. John] Harvard Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02115 USA. [Dawson-Hughes, Bess] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Bischoff-Ferrari, HA (reprint author), Univ Zurich Hosp, Dept Geriatr & Aging Res, Raemistr 100, CH-8091 Zurich, Switzerland. EM Heike.Bischoff@usz.ch NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 330 N WABASH AVE, STE 39300, CHICAGO, IL 60611-5885 USA SN 2168-6106 EI 2168-6114 J9 JAMA INTERN MED JI JAMA Intern. Med. PD JUN PY 2016 VL 176 IS 6 BP 865 EP 866 DI 10.1001/jamainternmed.2016.1629 PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA DN6KU UT WOS:000377184000045 PM 27273492 ER PT J AU Li, YS Tran, H Bundy, JW Burkey, TE Kerr, BJ Nielsen, MK Miller, PS AF Li, Y. S. Tran, H. Bundy, J. W. Burkey, T. E. Kerr, B. J. Nielsen, M. K. Miller, P. S. TI Evaluation of collection method and diet effects on apparent digestibility and energy values of swine diets SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE collection method; diet; digestibility; pig ID DISTILLERS DRIED GRAINS; AMINO-ACID DIGESTIBILITY; NEUTRAL DETERGENT FIBER; GROWING-PIGS; NUTRIENT DIGESTIBILITY; METABOLIZABLE ENERGY; FEED-INTAKE; NITROGEN LOSSES; CANOLA-MEAL; SOLUBLES AB Two experiments were conducted to investigate the effects of collection method and diet type on digestibility coefficients. In Exp. 1, 24 barrows were fed either a corn-soybean meal (CSBM) diet or CSBM with 20% dried distillers' grains with solubles (CSBM-DDGS). In Exp. 2, the effects of basal diet and collection method on determination of dried distillers' grains with solubles (DDGS) digestibility were studied using 24 barrows. The 4 diets used in Exp. 2 were: a CSBM (basal 1), a barley-canola meal (BCM; basal 2), 80% basal 1 with 20% DDGS (CSBM-DDGS), and 80% basal 2 with 20% DDGS (BCM-DDGS). In both experiments, feces were collected using a time-based collection method (DY) or a "marker-to-marker" collection method (MM). Diets contained 0.5% of titanium dioxide (TiO2) for estimating digestibility using the index marker approach (IM). The apparent total tract digestibility (ATTD) of DM and GE were lower (P < 0.05) in the CSBM-DDGS diet than in the CSBM diet in Exp. 1 but were not different in Exp. 2. All the estimates of BCM-based diets were consistently lower (P < 0.05) than those of CSBM-based diets. In Exp. 1, digestibility coefficients determined by the DY and MM were not different from each other, whereas those estimates were lower (P < 0.05) using the IM than those using the total collection approach (TC; DY and MM). In Exp. 2, interactions (P < 0.05) were observed between diet type and method for dietary digestibility coefficients. Digestibility and energy values estimated by the DY and MM were not different in pigs fed CSBM-based diets and the BCM-DDGS diet, whereas those estimates were greater (P < 0.05) using the DY than those using the MM in pigs fed the BCM. There were no interactions between basal diet and method for estimating DDGS digestibility. The ATTD of DM and GE of DDGS using the MM were greater (P < 0.05) than those using the IM, and ATTD of N tended to be greater (P < 0.10) using the MM than that using the IM. All estimates using the DY were not different from those using the MM or the IM, except that DE of DDGS was greater (P < 0.05) using the DY than when using the IM. Digestibility estimates of DDGS were not affected by basal diets. The mean DE and ME (as-fed basis) of DDGS were 3,994 and 3,688 kcal/kg, respectively, when estimated using the basal 1 diet and were 3,919 and 3,547 kcal/ kg, respectively, when estimated using the basal 2 diet. In conclusion, both collection methods can be used to estimate energy and nutrient digestibility of diets and DDGS when using CSBM-based diets. C1 [Li, Y. S.; Tran, H.; Bundy, J. W.; Burkey, T. E.; Nielsen, M. K.; Miller, P. S.] Univ Nebraska, Dept Anim Sci, Lincoln, NE 68503 USA. [Kerr, B. J.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. RP Miller, PS (reprint author), Univ Nebraska, Dept Anim Sci, Lincoln, NE 68503 USA. EM pmiller1@unl.edu NR 33 TC 0 Z9 0 U1 8 U2 12 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUN PY 2016 VL 94 IS 6 BP 2415 EP 2424 DI 10.2527/jas2016-0275 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA DN9WF UT WOS:000377430700017 PM 27285917 ER PT J AU Lopez, R Pulsipher, GD Guerra-Liera, JE Soto-Navarro, SA Balstad, LA Petersen, MK Dhuyvetter, DV Brown, MS Krehbiel, CR AF Lopez, R. Pulsipher, G. D. Guerra-Liera, J. E. Soto-Navarro, S. A. Balstad, L. A. Petersen, M. K. Dhuyvetter, D. V. Brown, M. S. Krehbiel, C. R. TI Effects of fat and/or methionine hydroxy analog added to a molasses-urea-based supplement on ruminal and postruminal digestion and duodenal flow of nutrients in beef steers consuming low-quality lovegrass hay SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE beef cattle; digestion; fat; low-quality forage; methionine hydroxy analog ID INTAKE PROTEIN SUPPLEMENTATION; LIMITING AMINO-ACIDS; BODY CONDITION SCORE; DAIRY-COWS; PREPARTUM SUPPLEMENTATION; GROWING STEERS; RECYCLED UREA; MICROBIAL USE; FORAGE; PERFORMANCE AB Five crossbred beef steers (initial BW = 338.6 +/- 7.8 kg) fitted with ruminal and duodenal cannulas were used in a 5 x 5 Latin square design experiment to evaluate the effects of methionine hydroxy analog (MHA) and/or yellow grease (fat) added to a molasses-urea-based supplement on intake and characteristics of digestion. Steers were fed low-quality hay (long-stem lovegrass Eragrostis curvula: 3.3% CP, 76.8% NDF; DM basis) ad libitum and supplemented with 0.91 kg/d (as fed) of 1 of 4 supplements in a 2 x 2 + 1 factorial arrangement of treatments. Supplemental treatments were 1) control (no supplement, NC); 2) molasses-urea liquid supplement (U); 3) U containing (as-fed basis) 1.65% MHA (UM); 4) U containing (as-fed basis) 12% fat (UF); and 5) U containing (as-fed basis) 1.65% MHA and 12% fat (UMF). Total and forage OM intake (kg/d and as % of BW) increased (P < 0.01) with molasses-urea, decreased (P <= 0.04) with MHA, and were not affected (P = 0.61) with fat supplementation. Total tract NDF digestibility increased (P = 0.01) with molasses-urea supplementation, and was less (P = 0.01) for fat than for nonfat supplementation. Total and microbial N flowing to the duodenum increased (P = 0.01) with molasses-urea supplementation. Although, total N flowing to duodenum was not affected (P = 0.27), microbial N decreased (P = 0.01), and non-ammonia nonmicrobial N (NANMN) increased (P = 0.01) with fat supplementation. Extent of in situ OM and NDF digestibility at 96 h increased (P = 0.01) with molasses-urea supplementation, but were not affected (P >= 0.14) by either MHA or fat supplementation. Duodenal flow of total AA, essential AA, and nonessential AA increased (P <= 0.02) with molasses-urea supplementation. Total and nonessential serum AA concentration decreased (P < 0.01) with molasses-urea supplementation. Total ruminal VFA concentration increased (P = 0.01) with molasses-urea supplementation, and was not affected (P >= 0.14) by MHA or fat supplementation. Fat can be used in molasses-urea liquid supplements for cattle consuming low-quality forage to increase energy intake without negatively affecting forage intake or characteristics of digestion. However, adding MHA did not further improve the response to urea supplementation of cattle consuming low-quality forage. Conversely, the inclusion of MHA on urea supplement decreased forage intake. C1 [Lopez, R.; Soto-Navarro, S. A.; Petersen, M. K.] Univ Autonoma Chapingo, Dept Zootecnia, Chapingo 56230, Mexico. [Pulsipher, G. D.; Balstad, L. A.] New Mexico State Univ, Dept Anim & Range Sci, Las Cruces, NM 88003 USA. [Guerra-Liera, J. E.; Soto-Navarro, S. A.] Univ Autonoma Sinaloa, Fac Agron, Culiacan 80016, Mexico. [Dhuyvetter, D. V.] Ridley Nutr Solut, Mankato, MN 56001 USA. [Brown, M. S.] Global Anim Prod Inc, Amarillo, TX 79118 USA. [Krehbiel, C. R.] Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA. [Petersen, M. K.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. [Brown, M. S.] 3701 Airway Blvd, Amarillo, TX 79118 USA. RP Soto-Navarro, SA (reprint author), Univ Autonoma Chapingo, Dept Zootecnia, Chapingo 56230, Mexico.; Soto-Navarro, SA (reprint author), Univ Autonoma Sinaloa, Fac Agron, Culiacan 80016, Mexico. EM ssoto@nmsu.edu NR 53 TC 1 Z9 1 U1 3 U2 3 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUN PY 2016 VL 94 IS 6 BP 2485 EP 2496 DI 10.2527/jas2015-0228 PG 12 WC Agriculture, Dairy & Animal Science SC Agriculture GA DN9WF UT WOS:000377430700025 PM 27285925 ER PT J AU Stonecipher, CA Panter, KE Villalba, JJ AF Stonecipher, C. A. Panter, K. E. Villalba, J. J. TI Effect of protein supplementation on forage utilization by cattle in annual grass-dominated rangelands in the Channeled Scablands of eastern Washington SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE cattle; diet selection; foraging behavior; grazing; protein; weed control ID SOCIAL FACILITATION; SAGEBRUSH STEPPE; VOLUNTARY INTAKE; GRAZING ANIMALS; DIET CHOICE; GREAT-BASIN; SELECTION; SHEEP; PERFORMANCE; QUALITY AB Medusahead [Taeniatherum caputmedusae (L.) Nevski] has become a major invasive plant on the annual grass-dominated rangelands within the Channeled Scablands of eastern Washington. Livestock typically avoid grazing medusahead, and forage alternatives are becoming limited in the region. Our hypothesis was that supplying a high-CP supplement would provide a nutritional context that complements the nutritional composition of medusahead and other annual grasses and thus aid cattle in utilizing this vegetation component, making grazing a more effective method of weed control. Cattle grazed annual grass-infested rangelands dominated with medusahead for 10-d periods during June, July, and August over 2 consecutive years. Eight separate pastures were grazed by cattle pairs during each of the 3 grazing periods. Cattle in 4 control pastures received no supplement and cattle in another 4 pastures received a supplement of canola meal that supplied 75% of the daily recommended CP requirement. Bite counts were used to determine diet composition. Forage categories consisted of annual grasses, perennial grasses, and forbs. Bites taken of annual grasses were similar between treatment groups during the first 5 d of the grazing period (P > 0.05), and then cattle supplemented with canola meal increased consumption of annual grasses, during d 6 to 10 of the grazing periods, over nonsupplemented animals (P < 0.05). Consumption of annual grasses was greater during the second year of the grazing study (P < 0.05), likely due to a decline in abundance of forage alternatives in the plant community. The percentage of medusahead in the annual grass forage class tended to decrease in grazed pastures over the 3 yr of the study (P = 0.056): 87 +/- 4.2, 64 +/- 3.6, and 50 +/- 3.6%, respectively. The percentage of medusahead in the annual grasses was similar across years in nongrazed pastures (P > 0.05). Forb production was greatest the first year of grazing and declined the second year of grazing and continued to decline the following year with no grazing (P < 0.05). Perennial grass production was low throughout the study. The effects of grazing on medusahead abundance suggest cattle may be used to graze this weed after it has matured in an integrated management program with other forms of control to reduce infestation prior to seeding with desirable forage species. C1 [Stonecipher, C. A.; Panter, K. E.] USDA ARS, Poisonous Plant Res Lab, Logan, UT 84341 USA. [Villalba, J. J.] Utah State Univ, Wildland Resources Dept, Logan, UT 84322 USA. RP Stonecipher, CA (reprint author), USDA ARS, Poisonous Plant Res Lab, Logan, UT 84341 USA. EM clint.stonecipher@ars.usda.gov RI Villalba, Juan/A-5836-2010 OI Villalba, Juan/0000-0001-8868-8468 NR 56 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUN PY 2016 VL 94 IS 6 BP 2572 EP 2582 DI 10.2527/jas2016-0369 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA DN9WF UT WOS:000377430700033 PM 27285933 ER PT J AU Grayson, AL Shackelford, SD King, DA McKeith, RO Miller, RK Wheeler, TL AF Grayson, A. L. Shackelford, S. D. King, D. A. McKeith, R. O. Miller, R. K. Wheeler, T. L. TI The effects of degree of dark cutting on tenderness and sensory attributes of beef SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE beef; beef flavor; dark cutting; tenderness ID WATER-HOLDING CAPACITY; ULTIMATE PH; SARCOMERE-LENGTH; BOVINE MUSCLES; FED STEERS; LONGISSIMUS TENDERNESS; POSTMORTEM PROTEOLYSIS; TARGETED CATTLE; MEAT TENDERNESS; OVINE MUSCLE AB The objective of this study was to determine the effect of degree of dark cutting (DC) on the tenderness, juiciness, and flavor attributes of beef. During carcass grading at a large U.S. commercial beef harvesting facility, DC carcasses (n = 160) and matching normal control (NC) carcasses (n = 160) were selected. Longissimus lumborum (LL) pH was determined and DC carcasses were classified as severe (severe dark cutter [SEDC]; mean pH = 6.9; n = 40), moderate (moderate dark cutter [MODC]; mean pH = 6.6; n = 40), mild (mild dark cutter [MIDC]; mean pH = 6.4; n = 40), or shady (shady dark cutter [SHDC]; mean pH = 6.1; n = 40). Strip loins were obtained from the left carcass sides, vacuum-packaged, and aged a 1 degrees C. Slice shear force (SSF) was measured (14 d postmortem) fresh (never frozen), and trained descriptive sensory analysis of tenderness, juiciness, and flavor was measured (13 d postmortem) on frozen/thawed LL steaks. Cooked SSF pieces were frozen and used for western blotting of desmin to determine extent of postmortem proteolysis. Thaw and cook loss decreased as intensity of DC increased, with SEDC having the lowest loss (1.83 and 10.1%, respectively) compared with NC (3.37 and 14.9%, respectively). Slice shear force was higher (P < 0.05) for SHDC (25.6 kg) and MIDC (22.9 kg) compared with SEDC (16.8 kg), MODC (19.4 kg), and NC (17.8 kg). Sarcomere length was shorter (P < 0.05) between DC class (1.66, 1.67, 1.71, and 1.73 mu m for SEDC, MODC, MIDC, and SHDC, respectively) and NC (1.86 mu m). Postmortem proteolysis of desmin was greater (P < 0.05) for NC compared with all DC classes (59.83% vs. 49.20, 40.31, 42.07, and 43.30% for SEDC, MODC, MIDC, and SHDC, respectively). Trained sensory panel ratings for tenderness differed (P < 0.05) among DC class with SEDC (6.51) the most tender followed by MODC (6.04) and then MIDC (5.19), whereas SHDC (4.66) and NC (4.93) were the toughest. Juiciness ratings differed (P < 0.05) among each DC class (5.9, 5.7, 5.4, and 5.2 for SEDC, MODC, MIDC, and SHDC, respectively), with no difference between MIDC or SHDC compared with NC (5.23). Fat-like, rancid, heated oil, chemical, and musty/earthy/hummus flavors increased (P < 0.05) whereas metallic, sour, and salty flavors decreased as severity of DC increased. This study showed DC and NC differed in LL tenderness, juiciness, and flavor. The direction and/or magnitude of those differences were greatly dependent on severity of DC. Steaks with intermediate pH (SHDC and MIDC) are most likely to be tough and are regularly included in U. S. Select and U. S. Choice product lines. C1 [Grayson, A. L.; McKeith, R. O.; Miller, R. K.] Texas A&M Univ, Texas A&M AgriLife Res, College Stn, TX 77843 USA. [Shackelford, S. D.; King, D. A.; Wheeler, T. L.] USDA ARS, Roman L Hruska US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. [Grayson, A. L.] Sadlers Smokehouse, Henderson, TX 75652 USA. [McKeith, R. O.] Coll Sequoias Tulare Coll Ctr, Div Agr, Tulare, CA 93274 USA. RP Wheeler, TL (reprint author), USDA ARS, Roman L Hruska US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. EM tommy.wheeler@ars.usda.gov NR 44 TC 0 Z9 0 U1 8 U2 13 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUN PY 2016 VL 94 IS 6 BP 2583 EP 2591 DI 10.2527/jas2016-0388 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA DN9WF UT WOS:000377430700034 PM 27285934 ER PT J AU Peterson, SC Chandrasekaran, SR Sharma, BK AF Peterson, Steven C. Chandrasekaran, Sriraam R. Sharma, Brajendra K. TI Birchwood biochar as partial carbon black replacement in styrene-butadiene rubber composites SO JOURNAL OF ELASTOMERS AND PLASTICS LA English DT Article DE Biochar; pyrolysis; carbon black; SBR; reinforcement ID NATURAL-RUBBER; FAST PYROLYSIS; REINFORCEMENT; ENHANCEMENT; MODULUS; SYSTEMS; WOOD AB Birchwood feedstock was used to make slow pyrolysis biochar that contained 89% carbon and <2% ash. This biochar was blended with carbon black (CB) as filler for styrene-butadiene rubber (SBR). Composites made from blended fillers of 25/75 biochar/CB were equivalent to or superior to their 100% CB analogs in terms of tensile strength and toughness at all filler concentrations. Composites made with 50/50 biochar/CB were equivalent to or superior to CB composites in tensile strength, elongation, and toughness properties at total filler concentrations up to 30%. These results demonstrate the potential to use renewable birchwood biochar to replace up to half the CB in certain SBR composites. C1 [Peterson, Steven C.] ARS, Plant Polymer Res, NCAUR, USDA, 1815 N Univ St, Peoria, IL 61604 USA. [Chandrasekaran, Sriraam R.; Sharma, Brajendra K.] Univ Illinois, Illinois Sustainable Technol Ctr, Champaign, IL 61820 USA. RP Peterson, SC (reprint author), ARS, Plant Polymer Res, NCAUR, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM steve.peterson@ars.usda.gov NR 23 TC 2 Z9 2 U1 5 U2 6 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0095-2443 EI 1530-8006 J9 J ELASTOM PLAST JI J. Elastomer Plast. PD JUN PY 2016 VL 48 IS 4 BP 305 EP 316 DI 10.1177/0095244315576241 PG 12 WC Materials Science, Multidisciplinary; Polymer Science SC Materials Science; Polymer Science GA DO2UR UT WOS:000377636100002 ER PT J AU Kim, S Adkins, J Biswas, A AF Kim, Sanghoon Adkins, Jason Biswas, Atanu TI Fabrication of latex rubber reinforced with micellar nanoparticle as an interface modifier SO JOURNAL OF ELASTOMERS AND PLASTICS LA English DT Article DE Nanoparticle; latex; interface modifier; tensile strength; hardness ID CYANOACRYLATE) NANOPARTICLES; GLIADIN; ZEIN AB Reinforced latex rubbers were fabricated by incorporating small amount of nanoparticles as interface modifier. The rubbers were fabricated in a compression mold at 130 degrees C. The incorporated nanoparticles were prepared from wheat protein (gliadin) and ethyl cyanoacrylate (ECA). These nanoparticles were disintegrated to the constituent amphiphilic block copolymers while mixing with latex suspension and adsorbed on the surface of latex particles. The disintegration of nanoparticles and adsorption of the constituent amphiphilic block copolymers on the surface of latex particles were investigated by dynamic light scattering. The improved mechanical properties of the produced reinforced latex rubbers were characterized by mechanical analysis (stress-strain) and hardness test. Incorporation of nanoparticle into latex improved most of the mechanical properties, such as tensile strength, % elongation, energy at break, and toughness by 30% to 300%. On the other hand, tensile modulus and hardness were not much affected by the incorporation of nanoparticles. This research showed that the optimum percentage of nanoparticle is 2%, and the optimum gliadin:ECA weight ratio is 3:1. C1 [Kim, Sanghoon; Adkins, Jason; Biswas, Atanu] ARS, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. RP Kim, S (reprint author), ARS, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM sanghoon.kim@ars.usda.gov NR 16 TC 0 Z9 0 U1 1 U2 1 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0095-2443 EI 1530-8006 J9 J ELASTOM PLAST JI J. Elastomer Plast. PD JUN PY 2016 VL 48 IS 4 BP 317 EP 330 DI 10.1177/0095244315576242 PG 14 WC Materials Science, Multidisciplinary; Polymer Science SC Materials Science; Polymer Science GA DO2UR UT WOS:000377636100003 ER PT J AU Sharma, M Millner, PD Hashem, F Camp, M Whyte, C Graham, L Cotton, CP AF Sharma, Manan Millner, Patricia D. Hashem, Fawzy Camp, Mary Whyte, Celia Graham, Lorna Cotton, Corrie P. TI Survival and Persistence of Nonpathogenic Escherichia coli and Attenuated Escherichia coli O157:H7 in Soils Amended with Animal Manure in a Greenhouse Environment SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Escherichia coli O157:H7; Manure; Persistence; Poultry litter; Soils ID PREHARVEST; OUTBREAK; LETTUCE AB Animal manure provides benefits to agriculture but may contain pathogens that contaminate ready-to-eat produce. U.S. Food and Drug Administration standards include 90- or 120-day intervals between application of manure and harvest of crop to minimize risks of pathogen contamination of fresh produce. Data on factors affecting survival of Escherichia coli in soils under greenhouse conditions are needed. Three separate studies were conducted to evaluate survival of nonpathogenic E. coli (gEc) and attenuated E. coli O157:H7 (attO157) inoculated at either low (4 log CFU/ml) or high (6 log CFU/ml) populations over 56 days. Studies involved two pot sizes (small, 398 cm(3); large, 89 liters), three soil types (sandy loam, SL; clay loam, CL; silt loam, SIL), and four amendments (poultry litter, PL; dairy manure liquids, DML; horse manure, HM; unamended). Amendments were applied to the surface of the soil in either small or large containers. Study 1, conducted in regularly irrigated small containers, showed that populations of gEc and attO157 (2.84 to 2.88 log CFU/g) in PL-amended soils were significantly (P < 0.05) greater than those in DML-amended (0.29 to 0.32 log CFU/g [dry weight] [gdw]) or unamended (0.25 to 0.28 log CFU/gdw) soils; soil type did not affect E. coli survival. Results from study 2, in large pots with CL and SIL, showed that PL-amended soils supported significantly higher attO157 and gEc populations compared with HM-amended or unamended soils. Study 3 compared results from small and large containers that received high inoculum simultaneously. Overall, in both small and large containers, PL amended soils supported higher gEc and attO157 populations compared with HM-amended and unamended soils. Populations of attO157 were significantly greater in small containers (1.83 log CFU/gdw) than in large containers (0.65 log CFU/gdw) at week 8, perhaps because small containers received more regular irrigation than large pots. Regular irrigation of small pots may have affected E. coli persistence in manure-amended soils. Overall, PL-amended soils in both small and large containers supported E. coli survival at higher populations compared with DML-, HM-, or unamended soils. C1 [Sharma, Manan; Millner, Patricia D.] USDA, Environm Microbial & Food Safety Lab, ARS, Bldg 173 BARC East, Beltsville, MD 20705 USA. [Hashem, Fawzy; Whyte, Celia; Graham, Lorna; Cotton, Corrie P.] USDA, Northeast Area, Stat Grp, ARS, Bldg 005 BARC West,10300 Baltimore Ave, Beltsville, MD 20705 USA. [Camp, Mary] Univ Maryland Eastern Shore, Dept Agr, Princess Anne, MD 21853 USA. RP Sharma, M (reprint author), USDA, Environm Microbial & Food Safety Lab, ARS, Bldg 173 BARC East, Beltsville, MD 20705 USA. EM manan.sharma@ars.usda.gov FU USDA-ARS Environmental Microbial Food Safety Laboratory "Microbial Ecology on Produce" project [8042-32420-005-00]; USDA-ARS [8042-32420-005-22]; University of Maryland Eastern Shore [8042-32420-005-22]; U.S. Food and Drug Administration Produce Safety Staff [IAA 224-11-2046] FX This work was funded by the USDA-ARS Environmental Microbial Food Safety Laboratory "Microbial Ecology on Produce" project (#8042-32420-005-00) and a Specific Cooperative Agreement (SCA) between USDA-ARS and the University of Maryland Eastern Shore (project 8042-32420-005-22) through an Interagency Agreement with the U.S. Food and Drug Administration Produce Safety Staff (IAA 224-11-2046). NR 21 TC 2 Z9 2 U1 1 U2 9 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUN PY 2016 VL 79 IS 6 BP 913 EP 921 DI 10.4315/0362-028X.JFP-15-421 PG 9 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DO1RT UT WOS:000377557500003 PM 27296594 ER PT J AU Sevart, NJ Baumann, N Thippareddi, H Houser, TA Luchansky, JB Porto-Fett, ACS Marx, DB Acuff, GR Phebus, RK AF Sevart, N. J. Baumann, N. Thippareddi, H. Houser, T. A. Luchansky, J. B. Porto-Fett, A. C. S. Marx, D. B. Acuff, G. R. Phebus, R. K. TI Evaluating the Efficacy of Three US Department of Agriculture-Approved Antimicrobial Sprays for Reducing Shiga Toxin-Producing Escherichia coli Surrogate Populations on Bob Veal Carcasses SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Antimicrobial interventions; Carcass wash; Color; Shiga toxin-producing Escherichia coli; Veal ID LACTIC-ACID; HOT-WATER; SENSORY CHARACTERISTICS; SALMONELLA-ENTERICA; BEEF CARCASSES; CHILLED BEEF; GROUND-BEEF; REDUCTION; INTERVENTIONS; VALIDATION AB Effective antimicrobial intervention strategies to reduce Shiga toxin producing Escherichia coli (STEC) risks associated with veal are needed. This study evaluated the efficacy of lactic acid (4.5%, pH 2.0), Citrilow (pH 1.2), and Beefxide (2.25%, pH 2.3) for reducing STEC surrogates on prerigor and chilled bob veal carcasses and monitored the effects of these interventions on chilled carcass color. Dehided bob veal carcasses were inoculated with a five-strain cocktail of rifampin-resistant, surrogate E. coli bacteria. E. coli surrogates were enumerated after inoculation, after water wash, after prechill carcass antimicrobial spray application, after chilling for 24 h, and after postchill carcass antimicrobial spray application; carcass color was measured throughout the process. A standard carcass water wash (similar to 50 degrees C) reduced the STEC surrogate population by 0.9 log CFU/cm(2) (P <= 0.05). All three antimicrobial sprays applied to prerigor carcasses delivered an additional similar to 0.5-log reduction (P <= 0.05) of the surrogates. Chilling of carcasses for 24 h reduced (P <= 0.05) the surrogate population by an additional log cycles. The postchill application of the antimicrobial sprays provided no further reductions. Carcass L*, a*, and b* color values were not different (P > 0.05) among carcass treatments. Generally, the types and concentrations of the antimicrobial sprays evaluated herein did not negatively impact visual or instrumental color of chilled veal carcasses. This study demonstrates that warm water washing, followed by a prechill spray treatment with a low-pH chemical intervention, can effectively reduce STEC risks associated with veal carcasses; this provides processors a validated control point in slaughter operations. C1 [Sevart, N. J.; Baumann, N.; Houser, T. A.; Phebus, R. K.] Kansas State Univ, Dept Anim Sci & Ind, Manhattan, KS 66506 USA. [Thippareddi, H.] Univ Nebraska, Dept Food Sci & Technol, Lincoln, NE 68588 USA. [Marx, D. B.] Univ Nebraska, Dept Stat, Lincoln, NE 68588 USA. [Luchansky, J. B.; Porto-Fett, A. C. S.] ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Acuff, G. R.] Texas A&M Ctr Food Safety, 2501 Earl Rudder Freeway South,Suite 700, College Stn, TX 77845 USA. [Thippareddi, H.] Univ Georgia, Dept Poultry Sci, Athens, GA 30602 USA. RP Sevart, NJ (reprint author), Kansas State Univ, Dept Anim Sci & Ind, Manhattan, KS 66506 USA. EM nsevart@ksu.edu FU National Institute of Food and Agriculture, U.S. Department of Agriculture [2012-68003-30155] FX This is contribution no. 16-094-J from the Kansas Agricultural Experiment Station (Manhattan, KS). This material is based upon work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award no. 2012-68003-30155. Special thanks to Mr. John Wolf (Kansas State University Meat Abattoir Manager), the entire Kansas State University meat laboratory group, and Drs. Ron Pope and Luis Mendonca for their help in the execution of this project. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA or other participating academic institutions. NR 35 TC 0 Z9 0 U1 8 U2 11 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUN PY 2016 VL 79 IS 6 BP 956 EP 962 DI 10.4315/0362-028X.JFP-15-435 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DO1RT UT WOS:000377557500008 ER PT J AU Edrington, TS Loneragan, GH Genovese, KJ Hanson, DL Nisbet, DJ AF Edrington, T. S. Loneragan, G. H. Genovese, K. J. Hanson, D. L. Nisbet, D. J. TI Salmonella Persistence within the Peripheral Lymph Nodes of Cattle following Experimental Inoculation SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Cattle; Lymph node; Persistence; Salmonella ID GROUND-BEEF; PREVALENCE; CARRIAGE; ENTERICA; CULL AB Utilizing a transdermal method of inoculation developed in our laboratory, the duration of infection of Salmonella in the peripheral lymph nodes of steers was examined. Thirty-six Holstein steers (mean body weight of 137 kg) were inoculated with Salmonella Montevideo (day 0) on each lower leg and both sides of the back and abdomen. Calves were euthanized beginning at 6 h and subsequently on each of days 1, 2, 4, 7, 9, 11, 14, and 21 postinoculation (four animals each time). The subiliac, popliteal, and superficial cervical (prescapular) lymph nodes were collected and cultured (quantitatively and qualitatively) for the challenge strain of Salmonella. The challenge strain was detected via direct culture within the lymph nodes at 6 h postinoculation and on each subsequent necropsy date. Salmonella levels in lymph node were 0.8 to 1.8 log CFU/g. Lymph nodes were generally positive after enrichment culture throughout the experiment. Salmonella elimination appeared to begin approximately 14 days postinoculation. However, elimination was not completed by day 21; therefore, a second experiment was conducted identical to the first except that the time from inoculation to necropsy was extended. Salmonella was recovered via direct culture on each of the necropsy days, and results in general were similar to those of experiment I, except that on days 20, 24, and 28 isolates from serogroups C-2 and E-1 were identified in addition to the inoculation strain C-1 in multiple animals The data from both experiments indicate that after a single inoculation event, Salmonella would be completely cleared by approximately 28 days. Further research with expanded times between inoculation and necropsy is required for verification. C1 [Edrington, T. S.; Genovese, K. J.; Nisbet, D. J.] ARS, USDA, Food & Feed Safety Res Unit, 2881 F&B Rd, College Stn, TX 77845 USA. [Loneragan, G. H.; Hanson, D. L.] Texas Tech Univ, Dept Anim & Food Sci, Int Ctr Food Ind Excellence, POB 42141, Lubbock, TX 79409 USA. RP Edrington, TS (reprint author), ARS, USDA, Food & Feed Safety Res Unit, 2881 F&B Rd, College Stn, TX 77845 USA. EM tom.edrington@ars.usda.gov FU beef and veal producers and importers; U.S. Department of Agriculture, National Institutes of Food and Agriculture, National Integrated Food Safety Initiative [2011-51110-31081] FX This project was funded in part by beef and veal producers and importers through their $1-per-head checkoff and was produced for the Cattlemen's Beef Board and state beef councils by the National Cattlemen's Beef Association and by the U.S. Department of Agriculture, National Institutes of Food and Agriculture, National Integrated Food Safety Initiative award 2011-51110-31081. Dr. Loneragan has provided scientific advice to Zoetis (formerly Pfizer Animal Health, the manufacturer of certain products used in this study) and has on occasion billed for this service. Dr. Loneragan has also received honoraria for service on advisory boards and presentations. No other financial conflicts of interest are reported. NR 8 TC 0 Z9 0 U1 0 U2 0 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUN PY 2016 VL 79 IS 6 BP 1032 EP 1035 DI 10.4315/0362-028X.JFP-15-325 PG 4 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DO1RT UT WOS:000377557500018 PM 27296609 ER PT J AU Chen, J Park, B AF Chen, Jing Park, Bosoon TI Recent Advancements in Nanobioassays and Nanobiosensors for Foodborne Pathogenic Bacteria Detection SO JOURNAL OF FOOD PROTECTION LA English DT Review DE Detection; Food; Nanomaterials; Nanotechnology; Pathogen ID ENHANCED RAMAN-SCATTERING; ESCHERICHIA-COLI O157-H7; DESORPTION/IONIZATION MASS-SPECTROMETRY; SURFACE-PLASMON RESONANCE; LABEL-FREE DETECTION; POLYMERASE-CHAIN-REACTION; REAL-TIME PCR; SALMONELLA-TYPHIMURIUM; QUANTUM DOTS; RAPID DETECTION AB Bacterial pathogens are one of the leading causes of food safety incidents and product recalls worldwide. Timely detection and identification of microbial contamination in agricultural and food products is crucial for disease prevention and outbreak investigation. In efforts to improve and/or replace time-consuming and laborious "gold standards" for pathogen detection, numerous alternative rapid methods have been proposed in the past 15 years, with a trend toward incorporating nanotechnology and nanomaterials in food pathogen detection. This article is a review of the use of nanotechnology in various detection and sample preparation techniques and advancements in nanotechnology applications in food matrices. Some practical considerations in nanobioassay design are discussed, and the gaps between research status quo and market demands are identified. C1 [Chen, Jing; Park, Bosoon] USDA ARS, US Natl Poultry Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. RP Park, B (reprint author), USDA ARS, US Natl Poultry Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. EM bosoon.park@ars.usda.gov RI Chen, Jing/A-3995-2017 NR 139 TC 0 Z9 0 U1 34 U2 44 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUN PY 2016 VL 79 IS 6 BP 1055 EP 1069 DI 10.4315/0362-028X.JFP-15-516 PG 15 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DO1RT UT WOS:000377557500021 PM 27296612 ER PT J AU Hughes, SO Power, TG Beck, A Betz, D Calodich, S Goodell, LS Hill, LG Hill, R Jaramillo, JA Johnson, SL Lanigan, J Lawrence, A Martinez, AD Nesbitt, M Overath, I Parker, L Ullrich-French, S AF Hughes, Sheryl O. Power, Thomas G. Beck, Ashley Betz, Drew Calodich, Shirley Goodell, L. Suzanne Hill, Laura G. Hill, Rachael Jaramillo, J. Andrea Johnson, Susan L. Lanigan, Jane Lawrence, Adair Martinez, AnaMaria Diaz Nesbitt, Merrianneeta Overath, Irene Parker, Louise Ullrich-French, Sarah TI Strategies for Effective Eating Development-SEEDS: Design of an Obesity Prevention Program to Promote Healthy Food Preferences and Eating Self-Regulation in Children From Low-Income Families SO JOURNAL OF NUTRITION EDUCATION AND BEHAVIOR LA English DT Article DE childhood obesity; prevention program; family-based; child eating self-regulation; food preferences ID CONFIRMATORY FACTOR-ANALYSIS; ENERGY-INTAKE; CALORIC COMPENSATION; YOUNG-CHILDREN; BEHAVIOR QUESTIONNAIRE; FEEDING QUESTIONNAIRE; CHILDHOOD OBESITY; WEIGHT STATUS; PORTION SIZE; ABSENCE AB Objective: To develop a scientifically based childhood obesity prevention program supporting child eating self-regulation and taste preferences. This article describes the research methods for the Strategies for Effective Eating Development program. A logic model is provided that depicts a visual presentation of the activities that will be used to guide the development of the prevention program. Design: Randomized, controlled prevention program, pretest, posttest, 6 months, and 12 months. Setting: Two sites: Houston, TX and Pasco, WA. Each trial will last 7 weeks with 8-10 mother-child dyads in each arm (prevention and control). Participants: Recruitment at Head Start districts (Texas; n = 160) and Inspire Child Development Center including Early Childhood Education and Head Start (Washington; n = 160). Sixteen trials with 16-20 parent-child dyads per trial will provide adequate power to detect moderate effects. Intervention: Multicomponent family-based prevention program incorporating a dialogue approach to adult learning and self-determination theory. Main Outcome Measures: Child assessments will include observed taste preferences, caloric compensation, and eating in the absence of hunger. Parent assessments will include parent-reported feeding, feeding emotions, acculturation, child eating behaviors, child food preferences, and child dietary intake. Heights and weights will be measured for parent and child. Analysis: A multilevel growth modeling analysis will be employed to consider the nested nature of the data: time points (level 1) within families (level 2) within trials (level 3). C1 [Hughes, Sheryl O.; Jaramillo, J. Andrea] Baylor Coll Med, Childrens Nutr Res Ctr, ARS, USDA,Dept Pediat, Houston, TX 77030 USA. [Power, Thomas G.; Beck, Ashley; Betz, Drew; Calodich, Shirley; Hill, Laura G.; Hill, Rachael; Lanigan, Jane; Lawrence, Adair; Martinez, AnaMaria Diaz; Overath, Irene; Parker, Louise] Washington State Univ, Dept Human Dev, Pullman, WA 99164 USA. [Goodell, L. Suzanne] N Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. [Johnson, Susan L.] Univ Colorado, Dept Pediat, Sect Nutr, Sch Med, Aurora, CO USA. [Nesbitt, Merrianneeta] Washington State Univ, Off Provost, Pullman, WA 99164 USA. [Ullrich-French, Sarah] Washington State Univ, Dept Educ Leadership Sport Studies & Educ Counsel, Pullman, WA 99164 USA. RP Hughes, SO (reprint author), Baylor Coll Med, Childrens Nutr Res Ctr, 1100 Bates Ave, Houston, TX 77030 USA. EM shughes@bcm.edu FU US Department of Agriculture (USDA) [2011-68001-30009]; USDA/Agricultural Research Service (Cooperative Agreement) [6250-51000] FX This research is supported by funds from the US Department of Agriculture (USDA) (Grant USDA 2011-68001-30009). This work is also a publication of the USDA/Agricultural Research Service Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine (Houston, TX) funded in part by the USDA/Agricultural Research Service (Cooperative Agreement 6250-51000). The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement from the US Government. The authors would like to acknowledge the following people who were instrumental in collecting and coding the data for this study: Nilda Micheli, Sandra Lopez, and all of the students and staff who continue to support the authors' efforts. NR 76 TC 0 Z9 0 U1 13 U2 18 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1499-4046 EI 1878-2620 J9 J NUTR EDUC BEHAV JI J. Nutr. Educ. Behav. PD JUN PY 2016 VL 48 IS 6 BP 405 EP + DI 10.1016/j.jneb.2016.04.388 PG 15 WC Education, Scientific Disciplines; Nutrition & Dietetics SC Education & Educational Research; Nutrition & Dietetics GA DN9JU UT WOS:000377396600009 PM 27288192 ER PT J AU Wu, YP Thompson, D Aroian, KJ McQuaid, EL Deatrick, JA AF Wu, Yelena P. Thompson, Deborah Aroian, Karen J. McQuaid, Elizabeth L. Deatrick, Janet A. TI Commentary: Writing and Evaluating Qualitative Research Reports SO JOURNAL OF PEDIATRIC PSYCHOLOGY LA English DT Editorial Material DE methods; patient-centered; qualitative; theme ID DATA-ANALYSIS SOFTWARE; YOUNG-ADULTS; PERCEIVED BARRIERS; CHILDHOOD-CANCER; HEALTH RESEARCH; ADOLESCENTS; TRANSITION; CARE; TRUSTWORTHINESS; PSYCHOLOGY AB Objective To provide an overview of qualitative methods, particularly for reviewers and authors who may be less familiar with qualitative research. Methods A question and answer format is used to address considerations for writing and evaluating qualitative research. Results and Conclusions When producing qualitative research, individuals are encouraged to address the qualitative research considerations raised and to explicitly identify the systematic strategies used to ensure rigor in study design and methods, analysis, and presentation of findings. Increasing capacity for review and publication of qualitative research within pediatric psychology will advance the field's ability to gain a better understanding of the specific needs of pediatric populations, tailor interventions more effectively, and promote optimal health. C1 [Wu, Yelena P.] Univ Utah, Dept Family & Prevent Med, Div Publ Hlth, 375 Chipeta Way,Suite A, Salt Lake City, UT 84108 USA. [Wu, Yelena P.] Huntsman Canc Inst, Canc Control & Populat Sci, Salt Lake City, UT USA. [Thompson, Deborah] USDA ARS, Childrens Nutr Res Ctr, Dept Pediat Nutr, Baylor Coll Med, Washington, DC 20250 USA. [Aroian, Karen J.] Univ Cent Florida, Coll Nursing, Orlando, FL 32816 USA. [McQuaid, Elizabeth L.] Brown Univ, Dept Psychiat & Human Behav, Providence, RI 02912 USA. [Deatrick, Janet A.] Univ Penn, Sch Nursing, Philadelphia, PA 19104 USA. RP Wu, YP (reprint author), Univ Utah, Dept Family & Prevent Med, Div Publ Hlth, 375 Chipeta Way,Suite A, Salt Lake City, UT 84108 USA. EM yelena.wu@utah.edu FU National Cancer Institute of the National Institutes of Health [K07CA196985]; USDA/ARS [58-6250-0-008] FX This work was supported by National Cancer Institute of the National Institutes of Health (K07CA196985 to Y.W.). This work is a publication of the United States Department of Agriculture/Agricultural Research Center (USDA/ARS), Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas. It is also a publication of the USDA/ARS, Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas, and funded in part with federal funds from the USDA/ARS under Cooperative Agreement No. 58-6250-0-008 (to D.T.). The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement from the U.S. government. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. NR 85 TC 2 Z9 2 U1 3 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0146-8693 EI 1465-735X J9 J PEDIATR PSYCHOL JI J. Pediatr. Psychol. PD JUN PY 2016 VL 41 IS 5 BP 493 EP 505 DI 10.1093/jpepsy/jsw032 PG 13 WC Psychology, Developmental SC Psychology GA DO0CP UT WOS:000377447300001 PM 27118271 ER PT J AU Lowney, CA Graham, BD Spetich, MA Shifley, SR Saunders, MR Jenkins, MA AF Lowney, Christy A. Graham, Bradley D. Spetich, Martin A. Shifley, Stephen R. Saunders, Michael R. Jenkins, Michael A. TI Two decades of compositional and structural change in deciduous old-growth forests of Indiana, USA SO JOURNAL OF PLANT ECOLOGY LA English DT Article DE forest remnants; forest structure; long-term data; mortality; species diversity; stand development ID EASTERN UNITED-STATES; FALSE DISCOVERY RATE; BEECH-MAPLE FOREST; TREE MORTALITY; OAK FORESTS; APPALACHIAN MOUNTAINS; HURRICANE DISTURBANCE; WOODY DETRITUS; NORTH-AMERICA; FIRE HISTORY AB Aims Using a network of permanent plots, we determined how multiple old-growth forests changed over an 18-19-year period at a state-wide scale. This examination of change allowed us to assess how the compositional and structural stability of each forest varied with site characteristics (topography, physiography and productivity) and stochastic disturbance. Methods In 2011, we resampled 150 plots distributed across five old-growth hardwood forests in Indiana, USA that were originally sampled in 1992-1993. Within each plot, we relocated and remeasured the diameter at breast height (dbh) of all trees (>= 10.0 cm) present during the 1992-1993 sample, which allowed us to track their individual fates through time for growth and mortality calculations. Trees that grew to >= 10.0 cm dbh since plot establishment were designated as ingrowth. The dbh and species of all saplings (stems >= 2.0 cm but <10.0 cm dbh) were also recorded. For each forest, we calculated density (stems ha(-1)), basal area (BA; m(2) t-testsha(-1)) and importance value (relative density + relative BA)/2) of trees by species. For saplings, density per ha was calculated by species for each forest. We also calculated annual mortality rate (AMR) for three diameter classes (10-29.9, 30-59.9 and >= 60 cm) and species richness (S), evenness (E) and Shannon-Weiner diversity (H') for the tree and sapling layers. Differences between years were compared for each forest using paired t-tests and Wilcoxon signed rank tests. Important Findings Although we observed commonality in changes across some sites, our results suggest that these forests differ in their rates and trajectories of change. Changes in total stand BA and density varied across sites and were influenced by past disturbance and mortality rates. We observed a general increase in the overstory dominance of Acer saccharum coupled with a general decrease in the dominance of Quercus section Lobatae (red oak group) species. Mortality of overstory trees present in 1992-1993 ranged from 27% to 49% over the study period (mean AMR 1.6-3.7%). Most sites experienced greater mortality of early and mid-successional species, but one site experienced heavy mortality of Fagus grandifolia, a shade-tolerant late-successional species. Shade tolerant species, A. saccharum in particular, dominated the sapling layer at most sites. However, recruitment of this species into larger size classes did not occur uniformly across all sites and the species was comparatively uncommon at one site. Overall, our results suggest that old-growth remnants, even within a single state, cannot be viewed as equivalent units with regard to research or management. Stochastic disturbance events and surrounding land use may have amplified effects on small scattered remnants. Therefore, continued monitoring of these rare, but biologically important forests is critical to their long-term management and protection. C1 [Lowney, Christy A.; Graham, Bradley D.; Saunders, Michael R.; Jenkins, Michael A.] Purdue Univ, Dept Forestry & Nat Resources, 715 West State St, W Lafayette, IN 47907 USA. [Lowney, Christy A.; Graham, Bradley D.; Saunders, Michael R.; Jenkins, Michael A.] Purdue Univ, Hardwood Tree Improvement & Regenerat Ctr, 715 West State St, W Lafayette, IN 47907 USA. [Spetich, Martin A.] USDA Forest Serv, Arkansas Forestry Sci Lab, Southern Res Stn, POB 1270, Hot Springs, AR 71902 USA. [Shifley, Stephen R.] USDA Forest Serv, No Res Stn, 202 ABNR Bldg, Columbia, MO 65201 USA. RP Jenkins, MA (reprint author), Purdue Univ, Dept Forestry & Nat Resources, 715 West State St, W Lafayette, IN 47907 USA.; Jenkins, MA (reprint author), Purdue Univ, Hardwood Tree Improvement & Regenerat Ctr, 715 West State St, W Lafayette, IN 47907 USA. EM jenkinma@purdue.edu FU USDA Forest Service Northern and Southern Research Stations; Hardwood Tree Improvement and Regeneration Center (United States Department of Agriculture Forest Service Northern Research Station) [10-JV-11242311-073]; Department of Forestry and Natural Resources at Purdue University FX USDA Forest Service Northern and Southern Research Stations; the Hardwood Tree Improvement and Regeneration Center (United States Department of Agriculture Forest Service Northern Research Station) (10-JV-11242311-073); Department of Forestry and Natural Resources at Purdue University. NR 91 TC 0 Z9 0 U1 25 U2 35 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1752-9921 EI 1752-993X J9 J PLANT ECOL JI J. Plant Ecol. PD JUN PY 2016 VL 9 IS 3 BP 256 EP 271 DI 10.1093/jpe/rtv050 PG 16 WC Plant Sciences; Ecology SC Plant Sciences; Environmental Sciences & Ecology GA DO0CJ UT WOS:000377446700002 ER PT J AU Wang, B Khir, R Pan, ZL Wood, D Mahoney, NE El-Mashad, H Wu, BG Ma, HL Liu, XR AF Wang, Bei Khir, Ragab Pan, Zhongli Wood, Delilah Mahoney, Noreen E. El-Mashad, Hamed Wu, Bengang Ma, Haile Liu, Xingrong TI Simultaneous decontamination and drying of rough rice using combined pulsed light and holding treatment SO JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE LA English DT Article DE rough rice; pulsed light; drying; inactivation; Aspergillus flavus; milling quality ID ESCHERICHIA-COLI O157-H7; LISTERIA-MONOCYTOGENES; INACTIVATION; DISINFECTION; QUALITY; SEEDS AB BACKGROUND: Pulsed light (PL) technology has been proven effective in food disinfection. However, increasing the light intensity or treatment time could swiftly increase the temperature of the food product. Using the thermal effect in an appropriate way may achieve a simultaneous disinfection and drying effect. The objective of this study was to investigate the feasibility of simultaneous disinfection and drying of rough rice using PL and holding treatment. RESULTS: Freshly harvested rice samples were inoculated by Aspergillus flavus (A. flavus) and treated using PL under different intensities and durations followed by holding treatment. The PL treatment under intensity of 1.08 W cm(-2) for 21 s led to a reduction of 0.29 log cfu g(-1) on the population size of A. flavus spores. After holding treatment, a 5.2 log cfu g(-1) reduction was achieved. The corresponding total moisture removal reached 3.3% points. No adverse effect on milling quality was detected after the treatment. CONCLUSION: The obtained results revealed that the combined PL and holding treatment had good potential for successful application in the rice industry to simultaneously achieve disinfection and drying. (c) 2015 Society of Chemical Industry C1 [Wang, Bei; Khir, Ragab; Pan, Zhongli; El-Mashad, Hamed] Univ Calif Davis, Dept Biol & Agr Engn, One Shields Ave, Davis, CA 95616 USA. [Wang, Bei; Wu, Bengang; Ma, Haile; Liu, Xingrong] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China. [Khir, Ragab] Suez Canal Univ, Fac Agr, Dept Agr Engn, Ismailia 41522, Egypt. [Pan, Zhongli; Wood, Delilah; Mahoney, Noreen E.] USDA ARS WRRC, Hlth Processed Foods Res Unit, Albany, CA 94710 USA. RP Pan, ZL (reprint author), Univ Calif Davis, Dept Biol & Agr Engn, One Shields Ave, Davis, CA 95616 USA. EM zhongli.Pan@ars.usda.gov FU National Natural Science Foundation of China [31271966]; Jiangsu Province Natural Science Foundation for Youth Scholars [BK20150499] FX The research was conducted at UC Davis and WRRC, USDA-ARS. The authors thank the Farmers' Rice Cooperative for supplying rice samples and grant support by the National Natural Science Foundation of China (No. 31271966) and Jiangsu Province Natural Science Foundation for Youth Scholars (No. BK20150499). NR 31 TC 0 Z9 0 U1 10 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-5142 EI 1097-0010 J9 J SCI FOOD AGR JI J. Sci. Food Agric. PD JUN PY 2016 VL 96 IS 8 BP 2874 EP 2881 DI 10.1002/jsfa.7458 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DN6RP UT WOS:000377203800034 PM 26369934 ER PT J AU Davis, TJ Kline, DL Kaufman, PE AF Davis, Timothy J. Kline, Daniel L. Kaufman, Phillip E. TI Assessment of Aedes albopictus (Skuse) (Diptera: Culicidae) clutch size in wild and laboratory populations SO JOURNAL OF VECTOR ECOLOGY LA English DT Article DE Oviposition; fecundity; mosquito control; mosquito ecology; skip oviposition; clutch size ID TRISERIATUS DIPTERA; TREEHOLE MOSQUITO; WYEOMYIA-SMITHII; BODY SIZE; FECUNDITY; OVIPOSITION; FLORIDA; AEGYPTI; CONSEQUENCES; COMPETITION AB Aedes albopictus (Skuse) is an invasive mosquito species found across the southern U.S. with range expansion into many northern states. Intra-and interspecific larval competition have been evaluated for Ae. albopictus with respect to subsequent adult size, immature and adult survivability, and its capacity to vector pathogens as an adult. However, limited data are available on egg production as related to larval rearing conditions. Because Ae. albopictus is a container-inhabiting mosquito that oviposits in resource-limited habitats, it is found under variable density-dependent conditions. Therefore, we examined the impact of specific rearing conditions on Ae. albopictus clutch size and adult body size; comparing the egg production values and wing lengths from known developmental densities to those from field-collected populations. Field populations varied significantly among collection sites in mean clutch size (23 to 46). These clutch sizes were comparable to the mean clutch sizes of females reared at the larval densities of nine (20 eggs) and three (53 eggs) larvae per 3 ml of water in the laboratory. Field populations experienced density-dependent effects impacting adult mosquito size. Mosquitoes from the four sample sites had mean wing lengths of 1.99, 2.47, 2.51, and 2.54 mm, which were less than the mean wing length of mosquitoes reared at larval densities of three larvae per 3 ml of water (2.57 mm). C1 [Davis, Timothy J.; Kaufman, Phillip E.] Univ Florida, Dept Entomol & Nematol, POB 110620, Gainesville, FL 32611 USA. [Kline, Daniel L.] USDA ARS, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. RP Kaufman, PE (reprint author), Univ Florida, Dept Entomol & Nematol, POB 110620, Gainesville, FL 32611 USA. EM pkaufman@ufl.edu OI Kaufman, Phillip/0000-0001-6159-8358 NR 39 TC 0 Z9 0 U1 5 U2 8 PU SOC VECTOR ECOLOGY PI CORONA PA 1966 COMPTON AVE, CORONA, CA 92881 USA SN 1081-1710 EI 1948-7134 J9 J VECTOR ECOL JI J. Vector Ecol. PD JUN PY 2016 VL 41 IS 1 BP 11 EP 17 DI 10.1111/jvec.12188 PG 7 WC Entomology SC Entomology GA DN6XK UT WOS:000377219600002 PM 27232119 ER PT J AU Ravussin, E Redman, LM Rochon, J Das, SK Fontana, L Kraus, WE Romashkan, S Williamson, DA Meydani, SN Villareal, DT Smith, SR Stein, RI Scott, TM Stewart, TM Saltzman, E Klein, S Bhapkar, M Martin, CK Gilhooly, CH Holloszy, JO Hadley, EC Roberts, SB AF Ravussin, Eric Redman, Leanne M. Rochon, James Das, Sai Krupa Fontana, Luigi Kraus, William E. Romashkan, Sergei Williamson, Donald A. Meydani, Simin N. Villareal, Dennis T. Smith, Steven R. Stein, Richard I. Scott, Tammy M. Stewart, Tiffany M. Saltzman, Edward Klein, Samuel Bhapkar, Manju Martin, Corby K. Gilhooly, Cheryl H. Holloszy, John O. Hadley, Evan C. Roberts, Susan B. CA CALERIE Study Grp TI A 2-Year Randomized Controlled Trial of Human Caloric Restriction: Feasibility and Effects on Predictors of Health Span and Longevity (vol 70, pg 1097, 2015) SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES LA English DT Correction C1 [Ravussin, Eric; Redman, Leanne M.; Williamson, Donald A.; Smith, Steven R.; Stewart, Tiffany M.; Martin, Corby K.] Pennington Biomed Res Ctr, 6400 Perkins Rd, Baton Rouge, LA 70808 USA. [Rochon, James; Kraus, William E.; Bhapkar, Manju] Duke Clin Res Inst, Durham, NC USA. [Rochon, James; Kraus, William E.; Bhapkar, Manju] Duke Univ, Sch Med, Durham, NC USA. [Rochon, James] Rho Fed Syst, Chapel Hill, NC USA. [Das, Sai Krupa; Meydani, Simin N.; Scott, Tammy M.; Saltzman, Edward; Gilhooly, Cheryl H.; Roberts, Susan B.] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Fontana, Luigi; Villareal, Dennis T.; Stein, Richard I.; Klein, Samuel; Holloszy, John O.] Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA. [Fontana, Luigi] Univ Brescia, Sch Med, Dept Clin & Expt Sci, I-25121 Brescia, Italy. [Fontana, Luigi] CEINGE Biotecnol Avanzate, Naples, Italy. [Romashkan, Sergei; Hadley, Evan C.] NIA, Gateway Bldg,Suite 3C307, Bethesda, MD 20892 USA. [Smith, Steven R.] Florida Hosp, Translat Res Inst Metab & Diabet, Orlando, FL USA. [Smith, Steven R.] Sanford Burnham Med Res Inst, Orlando, FL USA. RP Hadley, EC (reprint author), NIA, Gateway Bldg,Suite 3C307, Bethesda, MD 20892 USA. EM ehadley@nih.gov NR 1 TC 0 Z9 0 U1 5 U2 5 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1079-5006 EI 1758-535X J9 J GERONTOL A-BIOL JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci. PD JUN PY 2016 VL 71 IS 6 BP 839 EP 840 DI 10.1093/gerona/glw056 PG 4 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA DO0RL UT WOS:000377485900019 ER PT J AU Steuernage, B Periyannan, SK Hernandez-Pinzon, I Witek, K Rouse, MN Yu, GT Hatta, A Ayliffe, M Bariana, H Jones, JDG Lagudah, ES Wulff, BBH AF Steuernage, Burkhard Periyannan, Sambasivam K. Hernandez-Pinzon, Inmaculada Witek, Kamil Rouse, Matthew N. Yu, Guotai Hatta, Asyraf Ayliffe, Mick Bariana, Harbans Jones, Jonathan D. G. Lagudah, Evans S. Wulff, Brande B. H. TI Rapid cloning of disease-resistance genes in plants using mutagenesis and sequence capture SO NATURE BIOTECHNOLOGY LA English DT Article ID STEM RUST RESISTANCE; HEXAPLOID WHEAT; EVOLUTION; IDENTIFICATION; UG99 AB Wild relatives of domesticated crop species harbor multiple, diverse, disease resistance (R) genes that could be used to engineer sustainable disease control. However, breeding R genes into crop lines often requires long breeding timelines of 5-15 years to break linkage between R genes and deleterious alleles (linkage drag). Further, when R genes are bred one at a time into crop lines, the protection that they confer is often overcome within a few seasons by pathogen evolution(1). If several cloned R genes were available, it would be possible to pyramid R genes(2) in a crop, which might provide more durable resistance(1). We describe a three-step method (MutRenSeq)-that combines chemical mutagenesis with exome capture and sequencing for rapid R gene cloning. We applied MutRenSeq to clone stem rust resistance genes Sr22 and Sr45 from hexaploid bread wheat. MutRenSeq can be applied to other commercially relevant crops and their relatives, including, for example, pea, bean, barley, oat, rye, rice and maize. C1 [Steuernage, Burkhard; Hernandez-Pinzon, Inmaculada; Witek, Kamil; Jones, Jonathan D. G.; Wulff, Brande B. H.] Sainsbury Lab, Norwich, Norfolk, England. [Steuernage, Burkhard; Yu, Guotai; Hatta, Asyraf; Wulff, Brande B. H.] John Innes Ctr, Norwich, Norfolk, England. [Periyannan, Sambasivam K.; Ayliffe, Mick; Lagudah, Evans S.] CSIRO, Agr, Canberra, ACT, Australia. [Rouse, Matthew N.] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Rouse, Matthew N.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Hatta, Asyraf] Univ Putra Malaysia, Dept Agr Technol, Serdang 43400, Malaysia. [Bariana, Harbans] Univ Sydney, Plant Breeding Inst, Cobbitty, NSW, Australia. RP Wulff, BBH (reprint author), Sainsbury Lab, Norwich, Norfolk, England.; Wulff, BBH (reprint author), John Innes Ctr, Norwich, Norfolk, England.; Lagudah, ES (reprint author), CSIRO, Agr, Canberra, ACT, Australia. EM evans.lagudah@csiro.au; brande.wulff@jic.ac.uk RI Rouse, Matthew/G-5474-2011; Wulff, Brande/C-7465-2013; Jones, Jonathan/J-5129-2012; Periyannan, Sambasivam/C-1722-2014 OI Wulff, Brande/0000-0003-4044-4346; Jones, Jonathan/0000-0002-4953-261X; FU Gatsby Charitable Foundation, UK; Two Blades Foundation, USA; Biotechnology and Biological Sciences Research Council, UK; Borlaug Global Rust Initiative (BGRI) Durable Rust Resistance in Wheat (DRRW) Project; Bill & Melinda Gates Foundation; UK Department for International Development; USDA-ARS National Plant Disease Recovery System; Grains Research and Development Corporation, Australia; Universiti Putra Malaysia (UPM), Malaysia; NBI Computing infrastructure for Science (CiS) group FX This research was supported by funds from the Gatsby Charitable Foundation, UK; Two Blades Foundation, USA; Biotechnology and Biological Sciences Research Council, UK; Borlaug Global Rust Initiative (BGRI) Durable Rust Resistance in Wheat (DRRW) Project (administered by Cornell University with a grant from the Bill & Melinda Gates Foundation and the UK Department for International Development); USDA-ARS National Plant Disease Recovery System; Grains Research and Development Corporation, Australia; and a fellowship to A.H. from Universiti Putra Malaysia (UPM), Malaysia. We are grateful to colleagues in The Sainsbury Laboratory and the Two Blades Foundation for helpful discussions. This research was supported in part by the NBI Computing infrastructure for Science (CiS) group and Dan MacLean's group by providing computational infrastructure. NR 21 TC 8 Z9 8 U1 22 U2 49 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1087-0156 EI 1546-1696 J9 NAT BIOTECHNOL JI Nat. Biotechnol. PD JUN PY 2016 VL 34 IS 6 BP 652 EP 655 DI 10.1038/nbt.3543 PG 4 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DO5TT UT WOS:000377846400033 PM 27111722 ER PT J AU Butte, NF Puyau, MR Wilson, TA Liu, Y Wong, WW Adolph, AL Zakeri, IF AF Butte, Nancy F. Puyau, Maurice R. Wilson, Theresa A. Liu, Yan Wong, William W. Adolph, Anne L. Zakeri, Issa F. TI Role of physical activity and sleep duration in growth and body composition of preschool-aged children SO OBESITY LA English DT Article ID ENERGY-EXPENDITURE; CHILDHOOD OBESITY; OLDER CHILDREN; SEDENTARY TIME; RISK-FACTORS; ADIPOSITY; ACCELEROMETER; METAANALYSIS; ASSOCIATION; ADOLESCENTS AB ObjectiveThe impact of physical activity patterns and sleep duration on growth and body composition of preschool-aged children remains unresolved. Aims were (1) to delineate cross-sectional associations among physical activity components, sleep, total energy expenditure (TEE), and body size and composition; and (2) to determine whether physical activity components, sleep, and TEE predict 1-year changes in body size and composition in healthy preschool-aged children. MethodsAnthropometry, body composition, accelerometry, and TEE by doubly labeled water were measured at baseline; anthropometry and body composition were repeated 1 year later (n=111). ResultsCross-sectionally, positive associations between sedentary activity and weight and fat-free mass (FFM) (P = 0.009-0.047), and a negative association between moderate-vigorous physical activity (MVPA) and percent fat mass (FM) (P = 0.015) were observed. TEE and activity energy expenditure (AEE) were positively associated with weight, body mass index (BMI), FFM, and FM (P = 0.0001-0.046). Prospectively, TEE, AEE, physical activity level, and MVPA, but not sedentary activity, were positively associated with changes in BMI (P = 0.0001-0.051) and FFM (P = 0.0001-0.037), but not percent FM. Sleep duration inversely predicted changes in FM (P = 0.005) and percent FM (P = 0.006). ConclusionsProspectively, MVPA, TEE, AEE, and physical activity level promote normal growth and accretion of FFM, whereas sleep duration inversely predicts changes in adiposity in preschool-aged children. C1 [Butte, Nancy F.; Puyau, Maurice R.; Wilson, Theresa A.; Liu, Yan; Wong, William W.; Adolph, Anne L.] USDA ARS, Dept Pediat, Baylor Coll Med, Childrens Nutr Res Ctr, Houston, TX USA. [Zakeri, Issa F.] Drexel Univ, Dept Epidemiol & Biostat, Philadelphia, PA 19104 USA. RP Butte, NF (reprint author), USDA ARS, Dept Pediat, Baylor Coll Med, Childrens Nutr Res Ctr, Houston, TX USA. EM nbutte@bcm.edu FU U.S. Department of Agriculture (USDA)/Agricultural Research Service (ARS) [58-6250-0-008]; National Institutes of Health (NIH) [R01 DK085163] FX This project has been funded with federal funds from the U.S. Department of Agriculture (USDA)/Agricultural Research Service (ARS) under Cooperative Agreement No. 58-6250-0-008 and National Institutes of Health (NIH) grant No. R01 DK085163. The contents of this publication do not necessarily reflect the views or policies of the USDA or NIH, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government. NR 40 TC 0 Z9 0 U1 6 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1930-7381 EI 1930-739X J9 OBESITY JI Obesity PD JUN PY 2016 VL 24 IS 6 BP 1328 EP 1335 DI 10.1002/oby.21489 PG 8 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA DO1EK UT WOS:000377521100022 PM 27087679 ER PT J AU Gast, RK Guraya, R Jones, DR Anderson, KE Karcher, DM AF Gast, Richard K. Guraya, Rupa Jones, Deana R. Anderson, Kenneth E. Karcher, Darrin M. TI Colonization of internal organs by Salmonella Enteritidis in experimentally infected laying hens housed in enriched colony cages at different stocking densities SO POULTRY SCIENCE LA English DT Article DE Salmonella Enteritidis; laying hens; conventional cages; enriched colony cages; stocking density ID ENTERICA SEROVAR ENTERITIDIS; CHICKEN REPRODUCTIVE-TRACT; DIFFERENT HOUSING SYSTEMS; NATIONAL CONTROL PROGRAM; COMMERCIAL LAYER FARMS; RISK-FACTORS; EGG CONTAMINATION; HORIZONTAL TRANSMISSION; OBLIGATORY VACCINATION; EUROPEAN-UNION AB Epidemiologic analyses have linked the frequency of human infections with Salmonella enterica subspecies enterica serovar Enteritidis to the consumption of contaminated eggs and thus to the prevalence of this pathogen in commercial egg-laying flocks. Contamination of the edible contents of eggs by Salmonella Enteritidis is a consequence of the colonization of reproductive tissues in systemically infected hens. The animal welfare implications of laying hen housing systems have been widely debated, but no definitive consensus has yet emerged about the food safety significance of poultry housing options. The present study sought to determine the effects of two different bird stocking densities on the invasion of internal organs by Salmonella Enteritidis in groups of experimentally infected laying hens housed in colony cages enriched with perching and nesting areas. In two trials, groups of laying hens were distributed at two different stocking densities into colony cages and (along with a group housed in conventional cages) orally inoculated with doses of 1.0 x 10(7) cfu of Salmonella Enteritidis. At 5 to 6 d post-inoculation, hens were euthanized and samples of internal organs were removed for bacteriologic culturing. For both trials combined, Salmonella Enteritidis was recovered at a significantly (P < 0.05) greater frequency from hens in enriched colony cages at the higher stocking density than at the lower density from livers (75.0% vs. 51.4%) and ovaries (51.4% vs. 30.6%). However, spleens from hens in enriched colony cages at the higher stocking density were significantly less often positive for Salmonella Enteritidis than from hens in conventional cages at that same density (90.3% vs. 68.1%). These results suggest that stocking density can influence the susceptibility of hens to Salmonella Enteritidis, but other housing systems parameters may also contribute to the outcome of infections. C1 [Gast, Richard K.; Guraya, Rupa; Jones, Deana R.] ARS, USDA, US Natl Poultry Res Ctr, Athens, GA 30605 USA. [Anderson, Kenneth E.] N Carolina State Univ, Dept Poultry Sci, Raleigh, NC 27695 USA. [Karcher, Darrin M.] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. RP Gast, RK (reprint author), ARS, USDA, US Natl Poultry Res Ctr, Athens, GA 30605 USA. EM Richard.Gast@ars.usda.gov NR 69 TC 0 Z9 0 U1 7 U2 11 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD JUN PY 2016 VL 95 IS 6 BP 1363 EP 1369 DI 10.3382/ps/pew037 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA DN9VF UT WOS:000377428100017 PM 26944976 ER PT J AU Bakst, MR Welch, GR Fetterer, R Miska, K AF Bakst, M. R. Welch, G. R. Fetterer, R. Miska, K. TI Impact of broiler egg storage on the relative expression of selected blastoderm genes associated with apoptosis, oxidative stress, and fatty acid metabolism SO POULTRY SCIENCE LA English DT Article DE egg storage; embryo mortality; SPIDES; hatchability; stage-specific gene expression ID CHICK QUALITY; CELL-DEATH; EMBRYO; INCUBATION; HATCHABILITY; CLEAVAGE AB Cool temperature storage of eggs prior to incubation is a frequent practice by commercial broiler hatcheries. However, continued storage beyond 7 d leads to a progressive increase in the rate of early embryonic mortality. In this study, we examined the relative expression of 31 genes associated with fatty acid metabolism (8), apoptosis (7), and oxidative stress (16) pathways to better understand the basis of embryo mortality during egg storage. A total of 642 broiler eggs in 2 separate trials were subjected to the following egg treatments: stored 4 d (Control 1, C1); stored 21 d but subjected to short periods of incubation during egg storage (SPIDES); stored un-manipulated 21 d (NonSPIDES, NS); and stored 4 d then incubated for 10 h to advance the embryos to the same developmental stages as the SPIDES embryos (Control 2, C2). Hatchability trials 277 eggs) confirmed the efficacy of SPIDES compared to NS treatments in both trials. To determine relative expression of 31 selected genes, 365 blastoderms were isolated, staged, and flash frozen in batches of 5 to 10 blastoderms per vial (7 vials per egg treatment) prior to RNA extractions. Analysis of gene expression was performed using qRT-PCR and the results presented as relative expression normalized to C1. The relative expression of genes in which the SPIDES and C2 treatments were significantly up-or down-regulated in tandem indicated that the stage-specific expression of those genes was maintained by the SPIDES treatment. This study provides the relative gene expressions of blastodermal cells before and after prolonged egg storage as well as insight as to how SPIDES impacts blastodermal cell gene expression. C1 [Bakst, M. R.; Welch, G. R.; Miska, K.] ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD 20705 USA. [Fetterer, R.] ARS, Anim Parasitol & Dis Lab, USDA, Beltsville, MD 20705 USA. RP Bakst, MR (reprint author), ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD 20705 USA. EM murray.bakst@ars.usda.gov NR 18 TC 0 Z9 0 U1 2 U2 2 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD JUN PY 2016 VL 95 IS 6 BP 1411 EP 1417 DI 10.3382/ps/pew038 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA DN9VF UT WOS:000377428100024 PM 26944957 ER PT J AU Kim, HW Yan, FF Hu, JY Cheng, HW Kim, YHB AF Kim, H. W. Yan, F. F. Hu, J. Y. Cheng, H. W. Kim, Y. H. B. TI Effects of probiotics feeding on meat quality of chicken breast during postmortem storage SO POULTRY SCIENCE LA English DT Article DE probiotics; postmortem storage; discoloration; proteolysis; breast muscle ID WATER-HOLDING CAPACITY; RELATIVE ORGAN WEIGHT; GROWTH-PERFORMANCE; BROILER-CHICKENS; BACILLUS-SUBTILIS; CLOSTRIDIUM-BUTYRICUM; MUSCLE; LICHENIFORMIS; EXTRACT; FRESH AB This study evaluated the effects of dietary probiotic supplement and postmortem storage on meat quality of chicken breast during retail display. A total of 35 birds were randomly obtained from 3 feeding groups (control without probiotic supplement, 250 ppm Sporulin, and 500 ppm PoultryStar). The probiotic supplement had no influence on feed conversion ratio and body weight gain, as well as body weight at 29 and 44 d (P > 0.05). After slaughter, each side of the breast muscles (M. Pectoralis major) was assigned to either one d or 5 d of postmortem storage. Probiotic supplement had no influence on the rate of pH decline of chicken breast muscles during the initial 6 h postmortem (P > 0.05). No interactions between probiotic supplement and postmortem storage on meat quality were found (P > 0.05). Postmortem storage decreased drip loss from 25.30 to 18.05% (P < 0.05). Probiotics-fed chicken groups, particularly PoultryStar treatment, had a higher myofibrillar fragmentation index than the control group (P < 0.05). However, shear force values were not affected by the probiotic treatments. Decreases in color and lipid stabilities of breast muscles were found during display (P < 0.05), but were not affected by the probiotic supplement (P > 0.05). Our result suggests that probiotic supplement had no adverse impacts on proteolysis and oxidative changes during 5 d postmortem display storage. C1 [Kim, H. W.; Kim, Y. H. B.] Purdue Univ, Dept Anim Sci, Meat Sci & Muscle Biol Lab, 915 W State St, W Lafayette, IN 47907 USA. [Yan, F. F.; Hu, J. Y.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. [Cheng, H. W.] ARS, Livestock Behav Res Unit, USDA, W Lafayette, IN 47907 USA. RP Kim, YHB (reprint author), Purdue Univ, Dept Anim Sci, Meat Sci & Muscle Biol Lab, 915 W State St, W Lafayette, IN 47907 USA. EM bradkim@purdue.edu NR 33 TC 0 Z9 0 U1 7 U2 11 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD JUN PY 2016 VL 95 IS 6 BP 1457 EP 1464 DI 10.3382/ps/pew055 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA DN9VF UT WOS:000377428100029 PM 26944974 ER PT J AU Simko, I AF Simko, Ivan TI High-Resolution DNA Melting Analysis in Plant Research SO TRENDS IN PLANT SCIENCE LA English DT Review ID SINGLE-NUCLEOTIDE POLYMORPHISM; MARKER-ASSISTED SELECTION; RICE ORYZA-SATIVA; BAR-HRM ANALYSIS; CURVE-ANALYSIS; MOLECULAR CHARACTERIZATION; PROTECTED DESIGNATION; AUTHENTICITY ANALYSES; DISEASE RESISTANCE; SMALL AMPLICONS AB Genetic and genomic studies provide valuable insight into the inheritance, structure, organization, and function of genes. The knowledge gained from the analysis of plant genes is beneficial to all aspects of plant research, including crop improvement. New methods and tools are continually being developed to facilitate rapid and accurate mapping, sequencing, and analyzing of genes. Here, I review the recent progress in the application of high-resolution melting (HRM) analysis of DNA, a method that allows detecting polymorphism in double stranded DNA by comparing profiles of melting curves. Use of HRM has expanded considerably in the past few years as the method was successfully applied for high-throughput genotyping, mapping genes, testing food products and seeds, and other areas of plant research. C1 [Simko, Ivan] ARS, USDA, US Agr Res Stn, 1636 E Alisal St, Salinas, CA 93905 USA. RP Simko, I (reprint author), ARS, USDA, US Agr Res Stn, 1636 E Alisal St, Salinas, CA 93905 USA. EM Ivan.Simko@ars.usda.gov NR 110 TC 2 Z9 2 U1 12 U2 28 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 1360-1385 J9 TRENDS PLANT SCI JI Trends Plant Sci. PD JUN PY 2016 VL 21 IS 6 BP 528 EP 537 DI 10.1016/j.tplants.2016.01.004 PG 10 WC Plant Sciences SC Plant Sciences GA DO4CB UT WOS:000377728300009 PM 26827247 ER PT J AU Zhou, L Vega, FE Tan, HW Lluch, AER Meinhardt, LW Fang, WP Mischke, S Irish, B Zhang, DP AF Zhou, Lin Vega, Fernando E. Tan, Huawei Lluch, Aixa E. Ramirez Meinhardt, Lyndel W. Fang, Wanping Mischke, Sue Irish, Brian Zhang, Dapeng TI Developing Single Nucleotide Polymorphism (SNP) Markers for the Identification of Coffee Germplasm SO TROPICAL PLANT BIOLOGY LA English DT Article DE Fluidigm; Coffea arabica; Coffea canephora; Molecular markers; Tropical agriculture ID GENE-EXPRESSION; ARABICA L.; DIVERSITY; CANEPHORA; TRANSCRIPTOME; BIOSYNTHESIS; PSILANTHUS; RUBIACEAE; VARIETIES; GENOMICS AB Coffee is one of the most widely consumed beverages and represents a multibillion-dollar global industry. Accurate identification of coffee cultivars is essential for efficient management, exchange, and use of coffee genetic resources. To date, a universal platform that can allow data comparison across different laboratories and genotyping platforms has not been developed by the coffee research community. Using expressed sequence tags (EST) of Coffea arabica, C. canephora and C. racemosa from public databases, we developed 7538 single nucleotide polymorphism (SNP) markers and selected 180 for validation using 25 C. arabica and C. canephora accessions from Puerto Rico. Based on the validation result, we designated a panel of 55 SNP markers that are polymorphic across the two species. The average minor allele frequency and information index of this SNP panel are 0.281 and 0.690, respectively. This panel enabled the differentiation of all tested accessions of C. canephora, which accounts for 79.2 % of the total polymorphism in the samples. Only 21.8 % of the polymorphic SNPs were detected in the 12 C. arabica cultivars, which, nonetheless, were able to unambiguously differentiate the 12 Arabica cultivars into ten unique genotypes, including two synonymous groups. Several local Puerto Rican cultivars with partial Timor pedigree, including Limani, Frontn, and TARS 18087, showed substantial genetic difference from the other common Arabica cultivars, such as Catuai, Borbn, and Mundo Nuevo. This coffee SNP panel provides robust and universally comparable DNA fingerprints, thus can serve as a genotyping tool to assist coffee germplasm management, propagation of planting material, and coffee cultivar authentication. C1 [Zhou, Lin; Vega, Fernando E.; Tan, Huawei; Meinhardt, Lyndel W.; Fang, Wanping; Mischke, Sue; Zhang, Dapeng] ARS, USDA, Sustainable Perennial Crops Lab, USDA ARS BARC W, 10300 Baltimore Ave,Bldg 001,Rm 223, Beltsville, MD 20705 USA. [Zhou, Lin; Tan, Huawei; Fang, Wanping] Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China. [Lluch, Aixa E. Ramirez] Puerto Rico Dept Agr, POB 10163, Santurce, PR 00908 USA. [Irish, Brian] ARS, USDA, Trop Agr Res Stn, Mayaguez, PR 00682 USA. RP Zhang, DP (reprint author), ARS, USDA, Sustainable Perennial Crops Lab, USDA ARS BARC W, 10300 Baltimore Ave,Bldg 001,Rm 223, Beltsville, MD 20705 USA. EM Dapeng.Zhang@ars.usda.gov OI Vega, Fernando E./0000-0001-8103-5640 NR 56 TC 0 Z9 0 U1 5 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1935-9756 EI 1935-9764 J9 TROP PLANT BIOL JI Trop. Plant Biol. PD JUN PY 2016 VL 9 IS 2 BP 82 EP 95 DI 10.1007/s12042-016-9167-2 PG 14 WC Plant Sciences SC Plant Sciences GA DN9YN UT WOS:000377436700003 ER PT J AU Psarska, S Nater, EA Kolka, RK AF Psarska, Sona Nater, Edward A. Kolka, Randall K. TI Impacts of Invasive Earthworms on Soil Mercury Cycling: Two Mass Balance Approaches to an Earthworm Invasion in a Northern Minnesota Forest SO WATER AIR AND SOIL POLLUTION LA English DT Article DE Mercury; Earthworms; Forest soil; Mass balance ID NORTHEASTERN UNITED-STATES; ORGANIC-MATTER; TEMPERATE FORESTS; HARDWOOD FORESTS; CARBON; USA; RESISTANCE; EVOLUTION; NITROGEN; ACCUMULATION AB Invasive earthworms perturb natural forest ecosystems that initially developed without them, mainly by consuming the forest floor (an organic rich surficial soil horizon) and by mixing the upper parts of the soil. The fate of mercury (Hg) formerly contained in the forest floor is largely unknown. We used two mass balance approaches (simple mass balance and geochemical mass balance) to quantitatively assess the impact of exotic earthworms on Hg cycling in forest soils and the fate of Hg formerly contained in the forest floor. Two sets of soils, collected in 2009 and 2014 from an earthworm invasion transect in north central Minnesota, were analyzed in this study. We observed a substantial loss of Hg from the soil system following earthworm invasion. Mass balance calculations showed that 35 to 65 % of the forest floor Hg was retained in the underlying mineral soil with the remainder unaccounted. The most likely explanation for the loss of forest floor Hg from the system is microbially induced volatilization of Hg by soil microbes and possibly by earthworm gut microbes during consumption of the forest floor. Our calculations suggest that exotic earthworms can substantially perturb mercury cycling in the topsoil. Forest floor Hg may accumulate within worm biomass; however, this process can account for only a small fraction (1 to 3 %) of the lost Hg. Additionally, we calculated the mass balance of organic carbon (C) due to Hg's high affinity for organic matter and found that a portion of the organic carbon also appears to be lost from the soil system. C1 [Psarska, Sona; Nater, Edward A.] Univ Minnesota, Dept Soil Water & Climate, 1991 Upper Buford Circle, St Paul, MN 55108 USA. [Kolka, Randall K.] US Forest Serv, No Res Stn, USDA, 1831 Hwy 169 East, Grand Rapids, MN 55744 USA. RP Psarska, S (reprint author), Univ Minnesota, Dept Soil Water & Climate, 1991 Upper Buford Circle, St Paul, MN 55108 USA. EM psars002@umn.edu NR 54 TC 0 Z9 0 U1 15 U2 18 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0049-6979 EI 1573-2932 J9 WATER AIR SOIL POLL JI Water Air Soil Pollut. PD JUN PY 2016 VL 227 IS 6 AR 205 DI 10.1007/s11270-016-2885-0 PG 12 WC Environmental Sciences; Meteorology & Atmospheric Sciences; Water Resources SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences; Water Resources GA DO0FY UT WOS:000377456000028 ER PT J AU Woli, P Hoogenboom, G Alva, A AF Woli, Prem Hoogenboom, Gerrit Alva, Ashok TI Simulation of potato yield, nitrate leaching, and profit margins as influenced by irrigation and nitrogen management in different soils and production regions SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Crop model; Modeling; Leaching; Potato; Profit; Potato model; Systems analysis ID WATER-USE EFFICIENCY; CROPPING SYSTEM; SUGAR-BEET; MODEL; FERTILIZER; RECOVERY; ENVIRONMENT; FREQUENCY; CROPS; USA AB The Columbia Basin in the Pacific Northwest is a highly productive area for potatoes in the United States. Here, nitrate is the most frequently documented groundwater contaminant, and the challenge of maximizing crop productivity while minimizing the nitrate pollution still remains. This study assessed the responses of tuber yield, nitrate leaching, and profit margin to irrigation water amount, irrigation interval, nitrogen application rate, and soil type using 30 years of historical weather data and two representative soils in three locations of this region. A potato model was used to simulate the response variables for a total of 7500 scenarios (5 irrigation intervals x 5 irrigation amounts x 5 nitrogen rates x 2 soil types x 30 years) for each location. The results showed that nitrate leaching was greater with a larger irrigation, a longer irrigation interval, a higher nitrogen rate, and a lighter soil. Tuber yield was larger with a smaller irrigation, a higher nitrogen rate, and a heavier soil. Profit margin was larger with a smaller irrigation and a heavier soil. The optimum amount of irrigation water for the study region was 400 mm, at which both tuber yields and profit margins were the largest with the nitrogen application rate of 336 kg ha(-1). The increase in leaching with a larger irrigation was smaller for a longer irrigation interval and a lighter soil but larger for a higher nitrogen rate. These findings might be helpful to potato growers in this region in identifying irrigation and nitrogen application rates aimed toward maximizing yields and profits while minimizing the nitrate contamination of groundwater. (C) 2016 Elsevier B.V. All rights reserved. C1 [Woli, Prem; Hoogenboom, Gerrit] Washington State Univ, AgWeatherNet Program, 24106 N Bunn Rd, Prosser, WA 99350 USA. [Alva, Ashok] USDA ARS, Agr Syst Res Unit, 1500 N Cent Ave, Sidney, MT 59270 USA. RP Woli, P (reprint author), Washington State Univ, AgWeatherNet Program, 24106 N Bunn Rd, Prosser, WA 99350 USA. EM premwoli@gmail.com FU USDA National Institute for Food and Agriculture FX We thank Sara Sanjani, a visiting scientist to AgWeatherNet from Ferdowsi University of Mashhad, Iran for making available the data for potato model calibration/evaluation and the USDA National Institute for Food and Agriculture for supporting this work through grant. NR 63 TC 0 Z9 0 U1 7 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD JUN PY 2016 VL 171 BP 120 EP 130 DI 10.1016/j.agwat.2016.04.003 PG 11 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DM9TM UT WOS:000376708600012 ER PT J AU Nichols, KL Del Grosso, SJ Derner, JD Follett, RF Archibeque, SL Stewart, CE Paustian, KH AF Nichols, Kristopher L. Del Grosso, Stephen J. Derner, Justin D. Follett, Ronald F. Archibeque, Shawn L. Stewart, Catherine E. Paustian, Keith H. TI Nitrous oxide and methane fluxes from cattle excrement on C3 pasture and C4-dominated shortgrass steppe SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Nitrous oxide; Methane; Shortgrass steppe; Urine; Feces; Greenhouse gas ID SOIL-WATER CONTENT; TRACE GAS FLUXES; N2O FLUXES; EMISSION FACTORS; PASTORAL SOILS; DUNG PATCHES; COW URINE; GRASSLAND; COLORADO; EXCRETA AB Cattle play a major role in nutrient cycling of grassland ecosystems through biomass removal and excrement deposition (urine and feces). We studied the effects of cattle excrement patches (urine at 430 and feces at 940 kg N ha(-1)) on nitrous oxide (N2O) and methane (CH4) fluxes using semi-static chambers on cool-season (C3), Bozoisky-select (Psathyrostachys juncea) pasture, and warm-season (C4)-dominated native rangeland of the shortgrass steppe (SGS) in northeastern Colorado. Nitrous oxide emission factors (EF; i.e., percent of added N emitted as N2O-N) did not differ between urine and feces on the C4-dominated native rangeland (0.11 and 0.10%) and C3 pasture (0.13 and 0.10%). These EFs are substantially less than the Intergovernmental Panel on Climate Change (IPCC) Tier 1 Default EF (2%) for manure deposited on pasture, indicating that during dry years the IPCC Tier 1 Default EF would result in a significant overestimation of emissions from excrement patches deposited on SGS C4-dominated native rangeland and 0 pasture. Over the first year of the study (19 June 2012-18 June 2013), cumulative CH4 uptake was 38% greater for urine (-1.49 vs. -1.08 kg CH4-C ha(-1))and 28% greater for control plots (-2.09 vs.-1.63 kg CH4-C ha(-1)) on C4-dominated native rangeland compared to C3 pasture. In contrast, feces patches were net sources of CH4 with emissions from the C3 pasture (0.64 kg CH4-C ha(-1)) 113% greater than the C4-dominated native rangeland (0.30 kg CH4-C ha(-1)). Conversion of C4-dominated native rangeland to C3 pasture can have long term effects on CH4 uptake; therefore consideration should be taken before implementing this management practice. Published by Elsevier B.V. C1 [Nichols, Kristopher L.; Del Grosso, Stephen J.; Follett, Ronald F.; Stewart, Catherine E.] ARS, USDA, Plains Area, Soil Management & Sugar Beet Res Unit, 2150 Ctr Ave,Bldg D, Ft Collins, CO 80526 USA. [Derner, Justin D.] ARS, USDA, Plains Area, Rangeland Resources Res Unit, 8408 Hildreth Rd, Cheyenne, WY 82009 USA. [Archibeque, Shawn L.] Colorado State Univ, Dept Anim Sci, Ft Collins, CO 80523 USA. [Paustian, Keith H.] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Paustian, Keith H.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. RP Nichols, KL (reprint author), ARS, USDA, Plains Area, Soil Management & Sugar Beet Res Unit, 2150 Ctr Ave,Bldg D, Ft Collins, CO 80526 USA. EM kris.nichols@ars.usda.gov RI Paustian, Keith/L-7593-2016; OI Stewart, Catherine/0000-0003-1216-0450 NR 53 TC 1 Z9 1 U1 13 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD JUN 1 PY 2016 VL 225 BP 104 EP 115 DI 10.1016/j.agee.2016.03.026 PG 12 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA DN1CH UT WOS:000376803400011 ER PT J AU Su, G Ma, P Nielsen, US Aamand, GP Wiggans, G Guldbrandtsen, B Lund, MS AF Su, G. Ma, P. Nielsen, U. S. Aamand, G. P. Wiggans, G. Guldbrandtsen, B. Lund, M. S. TI Sharing reference data and including cows in the reference population improve genomic predictions in Danish Jersey SO ANIMAL LA English DT Article DE genomic selection; reliability; Jersey cattle; reference population ID JOINT REFERENCE POPULATION; DAIRY-CATTLE POPULATIONS; RELATIONSHIP MATRIX; NORDIC HOLSTEIN; ALLELE FREQUENCIES; BREEDING VALUES; FULL PEDIGREE; MARKER PANELS; UNITED-STATES; ONE-STEP AB Small reference populations limit the accuracy of genomic prediction in numerically small breeds, such like Danish Jersey. The objective of this study was to investigate two approaches to improve genomic prediction by increasing size of reference population in Danish Jersey. The first approach was to include North American Jersey bulls in Danish Jersey reference population. The second was to genotype cows and use them as reference animals. The validation of genomic prediction was carried out on bulls and cows, respectively. In validation on bulls, about 300 Danish bulls (depending on traits) born in 2005 and later were used as validation data, and the reference populations were: (1) about 1050 Danish bulls, (2) about 1050 Danish bulls and about 1150 US bulls. In validation on cows, about 3000 Danish cows from 87 young half-sib families were used as validation data, and the reference populations were: (1) about 1250 Danish bulls, (2) about 1250 Danish bulls and about 1150 US bulls, (3) about 1250 Danish bulls and about 4800 cows, (4) about 1250 Danish bulls, 1150 US bulls and 4800 Danish cows. Genomic best linear unbiased prediction model was used to predict breeding values. De-regressed proofs were used as response variables. In the validation on bulls for eight traits, the joint DK-US bull reference population led to higher reliability of genomic prediction than the DK bull reference population for six traits, but not for fertility and longevity. Averaged over the eight traits, the gain was 3 percentage points. In the validation on cows for six traits (fertility and longevity were not available), the gain from inclusion of US bull in reference population was 6.6 percentage points in average over the six traits, and the gain from inclusion of cows was 8.2 percentage points. However, the gains from cows and US bulls were not accumulative. The total gain of including both US bulls and Danish cows was 10.5 percentage points. The results indicate that sharing reference data and including cows in reference population are efficient approaches to increase reliability of genomic prediction. Therefore, genomic selection is promising for numerically small population. C1 [Su, G.; Ma, P.; Guldbrandtsen, B.; Lund, M. S.] Aarhus Univ, Ctr Quantitat Genet & Genom, Dept Mol Biol & Genet, DK-8830 Tjele, Denmark. [Nielsen, U. S.] Seges, DK-8200 Aarhus, Denmark. [Aamand, G. P.] Nord Cattle Genet Evaluat, DK-8200 Aarhus, Denmark. [Wiggans, G.] ARS, USDA, Beltsville, MD 20705 USA. RP Su, G (reprint author), Aarhus Univ, Ctr Quantitat Genet & Genom, Dept Mol Biol & Genet, DK-8830 Tjele, Denmark. EM guosheng.su@mbg.au.dk FU VikingGenetics; Nordic Cattle Genetic Evaluation FX This work was performed within the project "Genomic in herds", funded by VikingGenetics and Nordic Cattle Genetic Evaluation. NR 44 TC 3 Z9 3 U1 0 U2 1 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1751-7311 EI 1751-732X J9 ANIMAL JI Animal PD JUN PY 2016 VL 10 IS 6 BP 1067 EP 1075 DI 10.1017/S1751731115001792 PG 9 WC Agriculture, Dairy & Animal Science; Veterinary Sciences SC Agriculture; Veterinary Sciences GA DN5RA UT WOS:000377126700022 PM 26330119 ER PT J AU Brent, CS Wang, MX Miao, YG Hull, JJ AF Brent, Colin S. Wang, Meixian Miao, Yun-Gen Hull, J. Joe TI ECDYSTEROID AND CHITINASE FLUCTUATIONS IN THE WESTERN TARNISHED PLANT BUG (Lygus hesperus) PRIOR TO MOLT INDICATE ROLES IN DEVELOPMENT SO ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY LA English DT Article DE Western tarnished plant bug; Lygus hesperus; molt; ecdysteroid; chitinase ID BETA-N-ACETYLGLUCOSAMINIDASE; LARVAL PUPAL TRANSFORMATION; INSECT BLATTELLA-GERMANICA; MISSISSIPPI RIVER DELTA; MANDUCA-SEXTA; BOMBYX-MORI; CHORISTONEURA-FUMIFERANA; DROSOPHILA-MELANOGASTER; HELICOVERPA-ARMIGERA; TRANSGENIC TOBACCO AB Vital physiological processes that drive the insect molt represent areas of interest for the development of alternative control strategies. The western tarnished plant bug (Lygus hesperus Knight) is a pest of numerous agronomic and horticultural crops but the development of novel control approaches is impeded by limited knowledge of the mechanisms regulating its molt. To address this deficiency, we examined the fundamental relationship underlying the hormonal and molecular components of ecdysis. At 27 degrees C L. hesperus exhibits a temporally controlled nymph-adult molt that occurs about 4 days after the final nymph-nymph molt with ecdysteroid levels peaking 2 days prior to the final molt. Application of exogenous ecdysteroids when endogenous levels had decreased disrupted the nymphal-adult molt, with treated animals exhibiting an inability to escape the old exoskeleton and resulting in mortality compared to controls. Using accessible transcriptomic data, we identified 10 chitinase-like sequences (LhCht), eight of which had protein motifs consistent with chitinases. Phylogenetic analyses revealed orthologous relationships to chitinases critical to molting in other insects. RT-PCR based transcript profiling revealed that expression changes to four of the LhChts was coordinated with the molt period and ecdysteroid levels. Collectively, our results support a role for ecdysteroid regulation of the L. hesperus molt and suggest that cuticle clearance is mediated by LhCht orthologs of chitinases that are essential to the molt process. These results provide the initial hormonal and molecular basis for future studies to investigate the specific roles of these components in molting. (C) 2016 Wiley Periodicals, Inc. C1 [Brent, Colin S.; Wang, Meixian; Hull, J. Joe] ARS, Arid Land Agr Ctr, USDA, Maricopa, AZ 85138 USA. [Wang, Meixian; Miao, Yun-Gen] Zhejiang Univ, Coll Anim Sci, Hangzhou 310003, Zhejiang, Peoples R China. RP Brent, CS (reprint author), ARS, Arid Land Agr Ctr, USDA, Maricopa, AZ 85138 USA. EM colin.brent@ars.usda FU China Scholarship Council; Cotton, Inc. [12-373] FX Grant sponsor: China Scholarship Council (to MW); Grant sponsor: Cotton, Inc. (to CSB and JJH); Grant number: 12-373. NR 76 TC 1 Z9 1 U1 0 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0739-4462 EI 1520-6327 J9 ARCH INSECT BIOCHEM JI Arch. Insect Biochem. Physiol. PD JUN PY 2016 VL 92 IS 2 BP 108 EP 126 DI 10.1002/arch.21322 PG 19 WC Biochemistry & Molecular Biology; Entomology; Physiology SC Biochemistry & Molecular Biology; Entomology; Physiology GA DN4IE UT WOS:000377027400002 PM 27192063 ER PT J AU Roy, SK Kwon, SJ Cho, SW Kamal, AHM Kim, SW Sarker, K Oh, MW Lee, MS Chung, KY Xin, ZG Woo, SH AF Roy, Swapan Kumar Kwon, Soo Jeong Cho, Seong-Woo Kamal, Abu Hena Mostafa Kim, Sang-Woo Sarker, Kabita Oh, Myeong-Won Lee, Moon-Soon Chung, Keun-Yook Xin, Zhanguo Woo, Sun-Hee TI Leaf proteome characterization in the context of physiological and morphological changes in response to copper stress in sorghum SO BIOMETALS LA English DT Article DE Sorghum; Copper; Leaf; Proteomics; 2-DE ID METAL AFFINITY-CHROMATOGRAPHY; EXCESS COPPER; HEAVY-METALS; OXIDATIVE STRESS; ORYZA-SATIVA; 2-DIMENSIONAL ELECTROPHORESIS; BINDING PROTEINS; GENE-EXPRESSION; SALT STRESS; CD STRESS AB Copper (Cu) is an essential micronutrient required for normal growth and development of plants; however, at elevated concentrations in soil, copper is also generally considered to be one of the most toxic metals to plant cells due to its inhibitory effects against many physiological and biochemical processes. In spite of its potential physiological and economical significance, molecular mechanisms under Cu stress has so far been grossly overlooked in sorghum. To explore the molecular alterations that occur in response to copper stress, the present study was performed in ten-day-old Cu-exposed leaves of sorghum seedlings. The growth characteristics were markedly inhibited, and ionic alterations were prominently observed in the leaves when the seedlings were exposed to different concentrations (0, 100, and 150 A mu M) of CuSO4. Using two-dimensional gels with silver staining, 643 differentially expressed protein spots (a parts per thousand yen1.5-fold) were identified as either significantly increased or reduced in abundance. Of these spots, a total of 24 protein spots (a parts per thousand yen1.5-fold) from Cu-exposed sorghum leaves were successfully analyzed by MALDI-TOF-TOF mass spectrometry. Of the 24 differentially expressed proteins from Cu-exposed sorghum leaves, 13 proteins were up-regulated, and 11 proteins were down-regulated. The abundance of most identified protein species, which function in carbohydrate metabolism, stress defense and protein translation, was significantly enhanced, while that of another protein species involved in energy metabolism, photosynthesis and growth and development were severely reduced. The resulting differences in protein expression patterns together with related morpho-physiological processes suggested that these results could help to elucidate plant adaptation to Cu stress and provide insights into the molecular mechanisms of Cu responses in C-4 plants. C1 [Roy, Swapan Kumar; Kwon, Soo Jeong; Kim, Sang-Woo; Sarker, Kabita; Woo, Sun-Hee] Chungbuk Natl Univ, Dept Crop Sci, 410 Seongbong Ro, Cheongju 361763, Chungbuk, South Korea. [Cho, Seong-Woo] Rural Dev Adm, Natl Inst Crop Sci, Div Crop Breeding Res, Wanju Gun, South Korea. [Kamal, Abu Hena Mostafa] Univ Texas Arlington, Dept Chem & Biochem, Arlington, TX 76019 USA. [Oh, Myeong-Won] Natl Acad Agr Sci, Rural Dev Adm, Natl Agrobiodivers Ctr, Jeonju, South Korea. [Lee, Moon-Soon] Chungbuk Natl Univ, Dept Ind Plant Sci & Technol, Cheongju, South Korea. [Chung, Keun-Yook] Chungbuk Natl Univ, Dept Environm & Biol Chem, Cheongju, South Korea. [Xin, Zhanguo] USDA ARS, Plant Stress & Germplasm Dev Unit, 3810 4th St, Lubbock, TX USA. RP Woo, SH (reprint author), Chungbuk Natl Univ, Dept Crop Sci, 410 Seongbong Ro, Cheongju 361763, Chungbuk, South Korea. EM shwoo@chungbuk.ac.kr RI Roy, Swapan Kumar/R-2427-2016; OI Roy, Swapan Kumar/0000-0002-3837-4011; Cho, Seong-Woo/0000-0001-7900-4781; Woo, Sun Hee/0000-0002-6219-1688 NR 90 TC 0 Z9 0 U1 8 U2 18 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0966-0844 EI 1572-8773 J9 BIOMETALS JI Biometals PD JUN PY 2016 VL 29 IS 3 BP 495 EP 513 DI 10.1007/s10534-016-9932-6 PG 19 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA DM9JM UT WOS:000376682100012 PM 27067443 ER PT J AU Mayer, AL Buma, B Davis, A Gagne, SA Loudermilk, EL Scheller, RM Schmiegelow, FKA Wiersma, YF Franklin, J AF Mayer, Audrey L. Buma, Brian Davis, Amelie Gagne, Sara A. Loudermilk, E. Louise Scheller, Robert M. Schmiegelow, Fiona K. A. Wiersma, Yolanda F. Franklin, Janet TI How Landscape Ecology Informs Global Land-Change Science and Policy SO BIOSCIENCE LA English DT Article DE climate change; land use; landscape ecology; policy; urbanization ID CLIMATE-CHANGE; ECOSYSTEM SERVICES; UNITED-STATES; CONSERVATION; FOREST; SCALE; BIODIVERSITY; COMMUNITIES; PATTERN; USA AB Landscape ecology is a discipline that explicitly considers the influence of time and space on the environmental patterns we observe and the processes that create them. Although many of the topics studied in landscape ecology have public policy implications, three are of particular concern: climate change; land use-land cover change (LULCC); and a particular type of LULCC, urbanization. These processes are interrelated, because LULCC is driven by both human activities (e.g., agricultural expansion and urban sprawl) and climate change (e.g., desertification). Climate change, in turn, will affect the way humans use landscapes. Interactions among these drivers of ecosystem change can have destabilizing and accelerating feedback, with consequences for human societies from local to global scales. These challenges require landscape ecologists to engage policymakers and practitioners in seeking long-term solutions, informed by an understanding of opportunities to mitigate the impacts of anthropogenic drivers on ecosystems and adapt to new ecological realities. C1 [Mayer, Audrey L.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. [Mayer, Audrey L.] Michigan Technol Univ, Dept Social Sci, Houghton, MI 49931 USA. [Buma, Brian] Univ Alaska Southeast, Dept Nat Sci, Juneau, AK USA. [Davis, Amelie] Miami Univ, Dept Geog, Oxford, OH USA. [Davis, Amelie] Miami Univ, Inst Environm & Sustainabil, Oxford, OH USA. [Gagne, Sara A.] Univ N Carolina, Dept Geog & Earth Sci, Charlotte, NC 28223 USA. [Loudermilk, E. Louise] US Forest Serv, Southern Res Stn, Ctr Forest Disturbance Sci, Athens, GA USA. [Scheller, Robert M.] Portland State Univ, Dept Environm Sci & Management, Portland, OR 97207 USA. [Schmiegelow, Fiona K. A.] Univ Alberta, Dept Renewable Resources, Whitehorse, YT, Canada. [Schmiegelow, Fiona K. A.] Univ Alberta, Div Appl Sci, Yukon Coll, Whitehorse, YT, Canada. [Wiersma, Yolanda F.] Mem Univ Newfoundland, Dept Biol, St John, NF, Canada. [Franklin, Janet] Arizona State Univ, Sch Geog Sci & Urban Planning, Tempe, AZ USA. RP Mayer, AL (reprint author), Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA.; Mayer, AL (reprint author), Michigan Technol Univ, Dept Social Sci, Houghton, MI 49931 USA. EM almayer@mtu.edu RI Scheller, Robert/B-3135-2009 OI Wiersma, Yolanda/0000-0003-4604-9240; FU Department of Defense, Strategic Environmental Research and Development Program [RC-2243] FX We would like to thank the members of US-IALE for participating in our survey on policy topics and providing valuable feedback at the 2014 conference in Anchorage, Alaska. We would also like to express our gratitude to Jonathan Bossenbroeck, Meg Krawchuk, and members of the executive committee of the US-IALE (Whalen Dillon, Louis Iverson, and Helene Wagner), and three anonymous reviewers form any helpful comments that improved this manuscript. Finally, we want to acknowledge Amy Daniels at USAID (formerly USDA Forest Service) for her involvement in survey development and implementation, as well as early discussions on this topic. Work on this manuscript by ELL was supported by the Department of Defense, Strategic Environmental Research and Development Program (project no. RC-2243). NR 91 TC 0 Z9 0 U1 18 U2 41 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0006-3568 EI 1525-3244 J9 BIOSCIENCE JI Bioscience PD JUN PY 2016 VL 66 IS 6 BP 458 EP 469 DI 10.1093/biosci/biw035 PG 12 WC Biology SC Life Sciences & Biomedicine - Other Topics GA DN6BU UT WOS:000377157000005 ER PT J AU Iseyemi, OO Farris, JL Moore, MT Choi, SE AF Iseyemi, Oluwayinka O. Farris, Jerry L. Moore, Matthew T. Choi, Seo-eun TI Nutrient Mitigation Efficiency in Agricultural Drainage Ditches: An Influence of Landscape Management SO BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY LA English DT Article DE Ditches; Nutrients; Flow rates; Runoff ID LOW-GRADE WEIRS; CONSTRUCTED WETLANDS; WATER-QUALITY; HYDROLOGICAL VARIABILITY; AQUATIC MACROPHYTES; RUNOFF; VEGETATION; CAPACITY; NITROGEN; LOADS AB Drainage systems are integral parts of agricultural landscapes and have the ability to intercept nutrient loading from runoff to surface water. This study investigated nutrient removal efficiency within replicated experimental agricultural drainage ditches during a simulated summer runoff event. Study objectives were to examine the influence of routine mowing of vegetated ditches on nutrient mitigation and to assess spatial transformation of nutrients along ditch length. Both mowed and unmowed ditch treatments decreased NO3 (-)-N by 79 % and 94 % and PO4 (3-) by 95 % and 98 %, respectively, with no significant difference in reduction capacities between the two treatments. This suggests occasional ditch mowing as a management practice would not undermine nutrient mitigation capacity of vegetated drainage ditches. C1 [Iseyemi, Oluwayinka O.; Farris, Jerry L.] Arkansas State Univ, Grad Program Environm Sci, Jonesboro, AR USA. [Moore, Matthew T.] USDA ARS, Natl Sedimentat Lab, Oxford, MS 38655 USA. [Choi, Seo-eun] Arkansas State Univ, Dept Math & Stat, Jonesboro, AR USA. RP Farris, JL (reprint author), Arkansas State Univ, Grad Program Environm Sci, Jonesboro, AR USA. EM jlfarris@astate.edu FU United States Department of Agriculture, ARS [58-6408-9-351]; Judd Hill Foundation FX The authors will like to thank Traci Hudson, Ethan Leonard, Geoffrey Pyne and Levi Hass for laboratory and technical assistance. We also thank Mr. Mike Johnson, ASU Farm Facility Manager and Arkansas State University College of Agriculture and Technology for providing the research field site. This research was funded by the United States Department of Agriculture, ARS Cooperative Agreement 58-6408-9-351 and the Judd Hill Foundation. NR 27 TC 0 Z9 0 U1 26 U2 35 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0007-4861 EI 1432-0800 J9 B ENVIRON CONTAM TOX JI Bull. Environ. Contam. Toxicol. PD JUN PY 2016 VL 96 IS 6 BP 750 EP 756 DI 10.1007/s00128-016-1783-x PG 7 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DN3SR UT WOS:000376984200009 PM 27022936 ER PT J AU Wells, LA Chung, W Anderson, NM Hogland, JS AF Wells, Lucas A. Chung, Woodam Anderson, Nathaniel M. Hogland, John S. TI Spatial and temporal quantification of forest residue volumes and delivered costs SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE forest biomass; stand characteristics; treatment residue; bioenergy; stand delineation ID BIOMASS; USA; TOPOGRAPHY; MANAGEMENT; BOUNDARY; COLORADO; MOISTURE; ENERGY; INDEX; FIRE AB Growing demand for bioenergy, biofuels, and bioproducts has increased interests in the utilization of biomass residues from forest treatments as feedstock. In areas with limited history of industrial biomass utilization, uncertainties in the quantity, distribution, and cost of biomass production and logistics can hinder the development of new bio-based industries. This paper introduces a new methodology to quantify and spatially describe delivered feedstock volumes and costs across landscapes of arbitrary size in ways that characterize operational and annual management decision-making. Using National Agricultural Imagery Program (NAIP) imagery, the forest is segmented into operational-level treatment units. A remote sensing model based on NAIP imagery and Forest Inventory and Analysis plot data are used to attribute treatment units with stand-level estimates of basal area, tree density, aboveground biomass, and quadratic mean diameter. These methods are applied to a study site in southwestern Colorado to assess the quantity and distribution of treatment residue for use in bioenergy production. Results from the case study demonstrate how this generalized approach can be used in the analysis and decision-making process when establishing new bioenergy industries that use forest residue as feedstock. C1 [Wells, Lucas A.] Univ Montana, Dept Forest Management, 32 Campus Way, Missoula, MT 59801 USA. [Chung, Woodam] Oregon State Univ, Dept Forest Engn Resources & Management, 276 Peavy Hall, Corvallis, OR 97331 USA. [Anderson, Nathaniel M.; Hogland, John S.] US Forest Serv, Rocky Mt Res Stn, USDA, 800 East Beckwith, Missoula, MT 59801 USA. [Wells, Lucas A.] Oregon State Univ, Dept Forest Engn Resources & Management, 068 Peavy Hall, Corvallis, OR 97331 USA. RP Chung, W (reprint author), Oregon State Univ, Dept Forest Engn Resources & Management, 276 Peavy Hall, Corvallis, OR 97331 USA. EM woodam.chung@oregonstate.edu FU Agriculture and Food Research Initiative from the USDA National Institute of Food and Agriculture [2011-10006-30357]; Biomass Research and Development Initiative from the USDA National Institute of Food and Agriculture [2011-10006-30357]; USFS Rocky Mountain Research Station Grant [10-JV-11221636-282]; University of Montana; Oregon State University FX This research was supported by the Agriculture and Food Research Initiative, Biomass Research and Development Initiative (Competitive Grant No. 2011-10006-30357) from the USDA National Institute of Food and Agriculture and a USFS Rocky Mountain Research Station Grant ( No. 10-JV-11221636-282) with significant cost match and in-kind support from the University of Montana and Oregon State University. NR 39 TC 0 Z9 0 U1 3 U2 6 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD JUN PY 2016 VL 46 IS 6 BP 832 EP 843 DI 10.1139/cjfr-2015-0451 PG 12 WC Forestry SC Forestry GA DN2IL UT WOS:000376887200009 ER PT J AU Buma, B Krapek, J Edwards, RT AF Buma, Brian Krapek, John Edwards, Rick T. TI Watershed-scale forest biomass distribution in a perhumid temperate rainforest as driven by topographic, soil, and disturbance variables SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE temperate rainforest; carbon; remote sensing; spatial distribution; biomass ID SOUTHEAST ALASKA; CARBON STOCKS; WINDTHROW DISTURBANCE; PACIFIC-NORTHWEST; BRITISH-COLUMBIA; COASTAL FOREST; YELLOW-CEDAR; OLD-GROWTH; PRODUCTIVITY; DYNAMICS AB Temperate rainforests are the most carbon dense forest ecosystem on the planet, with C stocks several times higher than most other forested biomes. While climatic and disturbance drivers of these C stocks are relatively well explored, the spatial distribution of those stocks at the scale of entire watersheds is less well known, particularly in perhumid rainforests where research has been minimal. This study explored biomass distributions across an entire watershed simultaneously, from ocean to glacial icefields, in Southeast Alaska. Utilizing LiDAR and ground surveys, biomass was modelled throughout the landscape and distributions are described statistically. The dominant driver of biomass distributions at this scale (controlling for elevation) was the flow of water through the landscape: areas of higher water accumulation typically had low biomass (often <10 Mg.ha(-1)), whereas well-drained areas supported biomass approaching 950 Mg.ha(-1). This relationship was strong at all elevations; only riparian locations (typically well-drained soils) maintained high biomass at low slopes. Exposure to stand-replacing disturbances, often a dominant driver, was only a minor factor. This work emphasizes the importance of water in temperate rainforests and the potentially significant impacts of changes to biomass given changes in precipitation (both increasing and decreasing) due to global climate change. C1 [Buma, Brian] Univ Alaska Southeast, Dept Nat Sci, 11120 Glacier Hwy, Juneau, AK 99801 USA. [Krapek, John] Univ Alaska, Sch Nat Resources & Extens, Fairbanks, AK 99775 USA. [Edwards, Rick T.] US Forest Serv, Juneau Forestry Sci Lab, Juneau, AK 99801 USA. RP Buma, B (reprint author), Univ Alaska Southeast, Dept Nat Sci, 11120 Glacier Hwy, Juneau, AK 99801 USA. EM Brian.Buma@uas.alaska.edu FU Alaska EPSCoR NSF award [OIA-1208927]; State of Alaska; Alaska Coastal Forest Center; McIntire-Stennis Cooperative Forestry Research Program FX Support for this research was partially from Alaska EPSCoR NSF award No. OIA-1208927, the State of Alaska, and the Alaska Coastal Forest Center with funds from the McIntire-Stennis Cooperative Forestry Research Program. We thank William Bottorf for fieldwork help and two anonymous reviewers and the Editor for their constructive comments. NR 45 TC 0 Z9 0 U1 5 U2 10 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD JUN PY 2016 VL 46 IS 6 BP 844 EP 854 DI 10.1139/cjfr-2016-0041 PG 11 WC Forestry SC Forestry GA DN2IL UT WOS:000376887200010 ER PT J AU Royo, AA Peterson, CJ Stanovick, JS Carson, WP AF Royo, Alejandro A. Peterson, Chris J. Stanovick, John S. Carson, Walter P. TI Evaluating the ecological impacts of salvage logging: can natural and anthropogenic disturbances promote coexistence? SO ECOLOGY LA English DT Article DE disturbance severity; diversity; salvage; tree regeneration; wind disturbance ID SOUTHERN BOREAL FOREST; WHITE-TAILED DEER; OLD-GROWTH FOREST; EASTERN DECIDUOUS FOREST; BEECH BARK DISEASE; WIND DISTURBANCE; CATASTROPHIC WIND; POSTFIRE SALVAGE; FIRE SEVERITY; EXPERIMENTAL HURRICANE AB Salvage logging following windthrow is common throughout forests worldwide even though the practice is often considered inimical to forest recovery. Because salvaging removes trees, crushes seedlings, and compacts soils, many warn this practice may delay succession, suppress diversity, and alter composition. Here, over 8 yr following windthrow, we experimentally evaluate how salvaging affects tree succession across 11 gaps in Eastern deciduous forests of Pennsylvania, wherein each gap was divided into salvaged and control (unsalvaged) halves. Our gaps vary in size and windthrow severity, and we explicitly account for this variation as well as variation in soil disturbance (i.e., scarification) resulting from salvaging so that our results would be generalizable. Salvage logging had modest and ephemeral impacts on tree succession. Seedling richness and density declined similarly over time in both salvaged and unsalvaged areas as individuals grew into saplings. The primary impact of salvaging on succession occurred where salvaging scarified soils. Here, salvaging caused 41 to 82% declines in sapling abundance, richness, and diversity, but these differences largely disappeared within 5 yr. Additionally, we documented interactions between windthrow severity and scarification. Specifically, low-severity windthrow and scarification combined reinforced dominance by shade-tolerant and browse-tolerant species (Acer pensylvanicum, Fagus grandifolia). In contrast, high windthrow severity and scarification together reduced the density of a fast-growing pioneer tree (Prunus pensylvanica) and non-tree vegetation cover by 75% and 26%, respectively. This reduction enhanced the recruitment of two mid-successional tree species, Acer rubrum and Prunus serotina, by 2 and 3-fold, respectively. Thus, our findings demonstrate that salvaging creates novel microsites and mitigates competing vegetation, thereby enhancing establishment of important hardwoods and promoting tree species coexistence. Our results, coupled with an assessment of 27 published post-windthrow salvage studies, suggest short-term studies may overestimate the impact of salvaging on regeneration. We conclude that the ecological costs and benefits of salvaging depend upon the variation in canopy and soil disturbance severity as well as the timescale at which effects are evaluated. Thus, our findings are inconsistent with the view that salvaging inexorably undermines plant diversity; rather we suggest salvaging can promote tree species coexistence within various contexts. C1 [Royo, Alejandro A.] USDA Forest Serv, Northern Res Stn, POB 267, Irvine, PA 16329 USA. [Peterson, Chris J.] Univ Georgia, Dept Plant Biol, Athens, GA 30602 USA. [Stanovick, John S.] USDA Forest Serv, Northern Res Stn, Newtown Sq, PA 19073 USA. [Carson, Walter P.] Univ Pittsburgh, Dept Biol Sci, Pittsburgh, PA 15260 USA. RP Royo, AA (reprint author), USDA Forest Serv, Northern Res Stn, POB 267, Irvine, PA 16329 USA. EM aroyo@fs.fed.us FU USDA Forest Service Northern Research Station FX This work was supported by the USDA Forest Service Northern Research Station. We are deeply indebted to Eric Baxter, Von Brown, Josh Hanson, Lance Meyen, Judd Piemme, Ted Salk Greg Sanford, Harry Steele, and Ernie Wiltsie for field sampling. We thank Michael Chips, Lane Eskew, Patricia Raymond, Steve Brewer, and Brian Palik and four anonymous reviewers for comments on prior versions of this manuscript. NR 127 TC 0 Z9 0 U1 15 U2 34 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0012-9658 EI 1939-9170 J9 ECOLOGY JI Ecology PD JUN PY 2016 VL 97 IS 6 BP 1566 EP 1582 DI 10.1890/15-1093.1 PG 17 WC Ecology SC Environmental Sciences & Ecology GA DN6XN UT WOS:000377219900019 PM 27459786 ER PT J AU Li, JR Luco, AL Ochietti, B Fadhil, I Camirand, A Reinhardt, TA St-Arnaud, R Muller, W Kremer, R AF Li, Jiarong Luco, Aimee-Lee Ochietti, Benoit Fadhil, Ibtihal Camirand, Anne Reinhardt, Timothy A. St-Arnaud, Rene Muller, William Kremer, Richard TI Tumoral Vitamin D Synthesis by CYP27B1 1-alpha-Hydroxylase Delays Mammary Tumor Progression in the PyMT-MMTV Mouse Model and Its Action Involves NF-kappa B Modulation SO ENDOCRINOLOGY LA English DT Article ID BREAST-CANCER CELLS; 25-HYDROXYVITAMIN D LEVELS; D-RECEPTOR; 1-ALPHA,25-DIHYDROXYVITAMIN D-3; HUMAN COLON; IN-VITRO; 1,25-DIHYDROXYVITAMIN D-3; SIGNALING PATHWAYS; SPLICE VARIANTS; EXPRESSION AB Biologically active vitamin D (1,25-dihydroxycholecalciferol or 1,25(OH)(2)D) is synthetized from inactive prohormone 25-hydroxycholecalciferol (25(OH)D) by the enzyme CYP27B1 1-alpha-hydroxylase in kidney and several extrarenal tissues including breast. Although the development of breast cancer has been linked to inadequate vitamin D status, the importance of bioactive vitamin D production within tumors themselves is not fully understood. To investigate the role of tumoral vitamin D production in mammary epithelial cell progression to breast cancer, we conducted a Cre-loxP-mediated Cyp27b1 gene ablation in the mammary epithelium of the polyoma middle T antigen-mouse mammary tumor virus (PyMT-MMTV) mouse breast cancer model. Targeted ablation of Cyp27b1 was accompanied by significant acceleration in initiation of spontaneous mammary tumorigenesis. In vivo, cell proliferation, angiogenesis, cell cycle progression, and survival markers were up-regulated in tumors by Cyp27b1 ablation, and apoptosis was decreased. AK thymoma (AKT) phosphorylation and expression of several components of nuclear factor kappa B (NF-kappa B), integrin, and signal transducer and activator of transcription 3 (STAT3) signaling pathways were increased in Cyp27b1-ablated tumors compared with nonablated controls. In vitro, 1,25(OH)(2)D treatment induced a strong antiproliferative action on tumor cells from both ablated and nonablated mice, accompanied by rapid disappearance of NF-kappa B p65 from the nucleus and segregation in the cytoplasm. In contrast, treatment with the metabolic precursor 25(OH)D was only effective against cells from nonablated mice. 25(OH)D did not inhibit growth of Cyp27b1-ablated cells, and their nuclear NF-kappa B p65 remained abundant. Our findings demonstrate that in-tumor CYP27B1 1-alpha-hydroxylase activity plays a crucial role in controlling early oncogene-mediated mammary carcinogenesis events, at least in part by modulating tumoral cell NF-kappa B p65 nuclear translocation. C1 [Li, Jiarong; Luco, Aimee-Lee; Ochietti, Benoit; Fadhil, Ibtihal; Camirand, Anne; Kremer, Richard] McGill Univ, Ctr Hlth, Dept Med, Montreal, PQ H3A 1A1, Canada. [Muller, William] McGill Univ, Goodman Canc Res Ctr, Montreal, PQ H3A 1A1, Canada. [Reinhardt, Timothy A.] USDA ARS, Natl Anim Dis Ctr, POB 70, Ames, IA 50010 USA. [St-Arnaud, Rene] Shriners Hosp Children, Genet Unit, Montreal, PQ H3G 1A6, Canada. RP Kremer, R (reprint author), McGill Univ, Ctr Hlth, Dept Med, Calcium Res Lab, Glen Site,1001 Blvd Decarie,Mail Drop EM1-3229, Montreal, PQ H4A 3J1, Canada. EM richard.kremer@mcgill.ca FU Canadian Institute for Health Research [MOP 10839]; Susan G. Komen Foundation [KG 100766] FX This work was supported by the Canadian Institute for Health Research Grant MOP 10839 and by the Susan G. Komen Foundation Grant KG 100766. NR 69 TC 0 Z9 0 U1 1 U2 5 PU ENDOCRINE SOC PI WASHINGTON PA 2055 L ST NW, SUITE 600, WASHINGTON, DC 20036 USA SN 0013-7227 EI 1945-7170 J9 ENDOCRINOLOGY JI Endocrinology PD JUN PY 2016 VL 157 IS 6 BP 2204 EP 2216 DI 10.1210/en.2015-1824 PG 13 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DN6VM UT WOS:000377214600004 PM 27119753 ER PT J AU Zhu, YD Li, H Bhatti, S Zhou, SP Yang, Y Fish, T Thannhauser, TW AF Zhu, Yingde Li, Hui Bhatti, Sarabjit Zhou, Suping Yang, Yong Fish, Tara Thannhauser, Theodore W. TI Development of a laser capture microscope-based single-cell-type proteomics tool for studying proteomes of individual cell layers of plant roots SO HORTICULTURE RESEARCH LA English DT Article ID ARABIDOPSIS ROOT; HAIR DEVELOPMENT; GENE ENCODES; PROTEIN; TOMATO; ALUMINUM; GROWTH; SPECIALIZATION; CYTOSKELETON; EXPRESSION AB Single-cell-type proteomics provides the capability to revealing the genomic and proteomics information at cell-level resolution. However, the methodology for this type of research has not been well-developed. This paper reports developing a workflow of laser capture microdissection (LCM) followed by gel-liquid chromatography-tandem mass spectrometry (GeLC-MS/MS)-based proteomics analysis for the identification of proteomes contained in individual cell layers of tomato roots. Thin-sections (similar to 10-mu m thick, 10 sections per root tip) were prepared for root tips of tomato germinating seedlings. Epidermal and cortical cells (5000-7000 cells per tissue type) were isolated under a LCM microscope. Proteins were isolated and then separated by SDS-polyacrylamide gel electrophoresis followed by in-gel-tryptic digestion. The MS and MS/MS spectra generated using nanoLC-MS/MS analysis of the tryptic peptides were searched against ITAG2.4 tomato protein database to identify proteins contained in each single-cell-type sample. Based on the biological functions, proteins with proven functions in root hair development were identified in epidermal cells but not in the cortical cells. Several of these proteins were found in Al-treated roots only. The results demonstrated that the cell-type-specific proteome is relevant for tissue-specific functions in tomato roots. Increasing the coverage of proteomes and reducing the inevitable cross-contamination from adjacent cell layers, in both vertical and cross directions when cells are isolated from slides prepared using intact root tips, are the major challenges using the technology in proteomics analysis of plant roots. C1 [Zhu, Yingde; Li, Hui; Bhatti, Sarabjit; Zhou, Suping] Tennessee State Univ, Dept Agr & Environm Sci, Coll Agr Human & Nat Sci, Nashville, TN 37209 USA. [Yang, Yong; Fish, Tara; Thannhauser, Theodore W.] USDA ARS, RW Holley Ctr Agr & Hlth, 538 Tower Rd, Ithaca, NY 14853 USA. RP Zhou, SP (reprint author), Tennessee State Univ, Dept Agr & Environm Sci, Coll Agr Human & Nat Sci, Nashville, TN 37209 USA. EM zsuping@tnstate.edu NR 40 TC 0 Z9 0 U1 7 U2 11 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2052-7276 J9 HORTIC RES-ENGLAND JI Hortic. Res.-England PD JUN 1 PY 2016 VL 3 AR 16026 DI 10.1038/hortres.2016.26 PG 8 WC Plant Sciences; Horticulture SC Plant Sciences; Agriculture GA DN3UK UT WOS:000376989100001 PM 27280026 ER PT J AU Akin, M Eyduran, E Reed, BM AF Akin, Meleksen Eyduran, Ecevit Reed, Barbara M. TI Using the CHAID Data Mining Algorithm for Tissue Culture Medium Optimization. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Akin, Meleksen] Oregon State Univ, Dept Hort, ALS 4017, Corvallis, OR 97331 USA. [Eyduran, Ecevit] Igdir Univ, Fac Agr, Dept Anim Sci, Biometry Genet Unit, Igdir, Turkey. [Reed, Barbara M.] USDA ARS, Natl Clonal Germplasm Repository, 33447 Peoria Rd, Corvallis, OR 97333 USA. EM akinm@oregonstate.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2016 VL 52 SU 1 MA P-2037 BP S66 EP S66 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA DN2XR UT WOS:000376926800163 ER PT J AU Dasgupta, K Thomson, JG AF Dasgupta, K. Thomson, J. G. TI Purple Is the New Orange - Next Generation Smart Fruits. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Dasgupta, K.; Thomson, J. G.] USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. [Dasgupta, K.] Univ Calif Davis, One Shields Ave, Davis, CA 95616 USA. EM kdasgupta@ucdavis.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2016 VL 52 SU 1 MA P-1020 BP S40 EP S40 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA DN2XR UT WOS:000376926800100 ER PT J AU Goodman, CL Li, YF Zhou, KL Ringbauer, J Lincoln, TR Stanley, D AF Goodman, Cynthia L. Li, Yaofa Zhou, Kaile Ringbauer, Joseph Lincoln, Tamra R. Stanley, David TI A Novel Squash Bug Cell Line. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Goodman, Cynthia L.; Ringbauer, Joseph; Stanley, David] ARS, USDA, BCIRL, 1503 S Providence Rd, Columbia, MO 65203 USA. [Li, Yaofa; Lincoln, Tamra R.] IPM Ctr Hebei Prov, Hebei Acad Agr & Forestry Sci, Plant Protect Inst, Baoding, Peoples R China. [Zhou, Kaile] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. EM Cindy.Goodman@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2016 VL 52 SU 1 MA A-2002 BP S42 EP S43 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA DN2XR UT WOS:000376926800105 ER PT J AU Hughes, SR Pinkelman, RJ Cox, EJ Jones, MA Lindquist, M Bang, SS AF Hughes, Stephen R. Pinkelman, Rebecca J. Cox, Elby J. Jones, Marjorie A. Lindquist, Mitch Bang, Sookie S. TI Construction of a Yeast Artificial Chromosome Containing a Yeast-codon-optimized Brazzein Gene and Its Expression in Saccharomyces cerevisiae SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Hughes, Stephen R.; Pinkelman, Rebecca J.; Cox, Elby J.; Jones, Marjorie A.; Lindquist, Mitch; Bang, Sookie S.] ARS, USDA, NCAUR, BBC, Room 1106,1815 North Univ St, Peoria, IL 61604 USA. EM Stephen.Hughes@ARS.USDA.GOV NR 0 TC 0 Z9 0 U1 2 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2016 VL 52 SU 1 MA P-1 BP S18 EP S18 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA DN2XR UT WOS:000376926800045 ER PT J AU Jenderek, MM Ledo, A AF Jenderek, Maria M. Ledo, Ana TI Antioxidants Improve Micropropagation and Cryopreservation. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Jenderek, Maria M.] USDA ARS, Plant & Anim Germplasm Preservat, NLGRP, Ft Collins, CO 80521 USA. [Ledo, Ana] Embrapa Coastal Tablelands, BR-49025040 Aracaju, Sergipe, Brazil. EM maria.jenderek@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2016 VL 52 SU 1 MA P-13 BP S22 EP S22 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA DN2XR UT WOS:000376926800056 ER PT J AU Kamo, K Lakshman, D Pandey, R Guaragna, MA Okubara, P Rajasekaran, K Cary, J Jordan, R AF Kamo, K. Lakshman, D. Pandey, R. Guaragna, M. A. Okubara, P. Rajasekaran, K. Cary, J. Jordan, R. TI Resistance to Fusarium oxysporum f. sp gladioli in Transgenic Gladiolus Plants Expressing Either a Bacterial Chloroperoxidase or Fungal Chitinase Genes SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Kamo, K.; Lakshman, D.; Pandey, R.; Guaragna, M. A.; Jordan, R.] USDA, Floral & Nursery Plants Res Unit, Natl Arboretum, Beltsville, MD 20705 USA. [Okubara, P.] USDA, Root Dis & Biol Control Res Unit, Pullman, WA USA. [Rajasekaran, K.; Cary, J.] USDA, Food & Feed Safety Res Unit, So Reg Res Ctr, New Orleans, LA USA. EM Kathryn.Kamo@ars.usda.gov NR 0 TC 0 Z9 0 U1 2 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2016 VL 52 SU 1 MA P-2025 BP S61 EP S62 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA DN2XR UT WOS:000376926800151 ER PT J AU Neugebauer, K Fellers, J Trick, HN AF Neugebauer, Kerri Fellers, J. Trick, H. N. TI Susceptibility and Race Specific Wheat Genes Induced by Six Puccinia triticina Races. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Neugebauer, Kerri; Trick, H. N.] Kansas State Univ, Dept Plant Pathol, 4024 Throckmorton Hall, Manhattan, KS 66506 USA. [Fellers, J.] USDA ARS GMPRC, 4006 Throckmorton Hall, Manhattan, KS 66506 USA. EM kerrin@ksu.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2016 VL 52 SU 1 MA P-1007 BP S35 EP S35 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA DN2XR UT WOS:000376926800088 ER PT J AU Pence, VC Niedz, RP Finke, L Bhargava, R AF Pence, Valerie C. Niedz, Randall P. Finke, Linda Bhargava, Radhika TI Improving In Vitro Growth of the Endangered Dryland Species, Arctomecon humilis. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Pence, Valerie C.; Niedz, Randall P.] Cincinnati Zoo & Bot Garden, Ctr Conservat & Res Endangered Wildlife CREW, 3400 Vine St, Cincinnati, OH 45220 USA. [Bhargava, Radhika] USDA ARS, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM valerie.pence@cincinnatizoo.org NR 0 TC 0 Z9 0 U1 2 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2016 VL 52 SU 1 MA P-2038 BP S66 EP S67 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA DN2XR UT WOS:000376926800164 ER PT J AU Scorza, R Dardick, C Callahan, A Bell, R Liu, ZR Srinivasan, C AF Scorza, Ralph Dardick, Chris Callahan, Ann Bell, Richard Liu, Zongrang Srinivasan, Chinnathambi TI Intersections of Plant Biotechnology and Fruit Tree Breeding. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Scorza, Ralph; Dardick, Chris; Callahan, Ann; Bell, Richard; Liu, Zongrang; Srinivasan, Chinnathambi] USDA ARS, Appalachian Fruit Res Lab, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM Ralph.scorza@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2016 VL 52 SU 1 MA P-9 BP S20 EP S21 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA DN2XR UT WOS:000376926800052 ER PT J AU Stanley, D Goodman, CL AF Stanley, David Goodman, Cynthia L. TI Prostaglandin Actions in Insect Immunity: Results from Primary Hemocyte Cultures SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Stanley, David; Goodman, Cynthia L.] ARS, USDA, BCIRL, 1503 S Providence Rd, Columbia, MO 65203 USA. EM David.Stanley@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2016 VL 52 SU 1 MA I-4 BP S16 EP S16 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA DN2XR UT WOS:000376926800041 ER PT J AU Stevens, ME Pijut, PM AF Stevens, Micah E. Pijut, Paula M. TI Meta-topolin Combined with Liquid Culture Improves Micropropagation of the Recalcitrant Species Black Walnut (Juglans nigra). SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Stevens, Micah E.] Purdue Univ, Dept Forestry & Nat Resources, HTIRC, 715 West State St, W Lafayette, IN 47907 USA. [Pijut, Paula M.] US Forest Serv, USDA, No Res Stn, HTIRC, 715 West State St, W Lafayette, IN 47907 USA. EM steven31@purdue.edu; ppijut@purdue.edu NR 0 TC 0 Z9 0 U1 4 U2 4 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2016 VL 52 SU 1 MA P-2040 BP S67 EP S67 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA DN2XR UT WOS:000376926800166 ER PT J AU Taylor, NJ Narayanan, N Beyene, G Chauhan, RD Gaitan-Solis, E Siritunga, D Grusak, MA Anderson, P AF Taylor, Nigel J. Narayanan, Narayanan Beyene, Getu Chauhan, Raj Deepika Gaitan-Solis, Eliana Siritunga, Dimuth Grusak, Michael A. Anderson, Paul TI Iron and Zinc Biofortification of Cassava Storage Roots to Nutritionally Significant Levels. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Taylor, Nigel J.; Narayanan, Narayanan; Beyene, Getu; Chauhan, Raj Deepika; Gaitan-Solis, Eliana; Anderson, Paul] Donald Danforth Plant Sci Ctr, 975 North Warson Rd, St Louis, MO 63132 USA. [Siritunga, Dimuth] Univ Puerto Rico, Dept Biol, Mayaguez, PR USA. [Grusak, Michael A.] USDA ARS, Dept Pediat, Baylor Coll Med, Houston, TX USA. EM ntaylor@danforthcenter.org RI Taylor, Nigel/M-2851-2013 NR 0 TC 0 Z9 0 U1 3 U2 4 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2016 VL 52 SU 1 MA P-1016 BP S38 EP S38 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA DN2XR UT WOS:000376926800096 ER PT J AU Sun, JH Zhang, ML Chen, P AF Sun, Jianghao Zhang, Mengliang Chen, Pei TI GLS-Finder: A Platform for Fast Profiling of Glucosinolates in Brassica Vegetables SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE GLS-Finder; glucosinolates; Brassica; vegetables; UHPLC-HRAM/MSn ID PERFORMANCE LIQUID-CHROMATOGRAPHY; MASS-SPECTROMETRY; BROCCOLI SPROUTS; ION-TRAP; DIETARY; ISOTHIOCYANATES; DISSOCIATION; INDUCERS; CANCER; PLANTS AB Mass spectrometry combined with related tandem techniques has become the most popular method for plant secondary metabolite characterization. We introduce a new strategy based on in-database searching, mass fragmentation behavior study, formula predicting for fast profiling of glucosinolates, a class of important compounds in brassica vegetables. A MATLAB script-based expert system computer program, "GLS-Finder", was developed. It is capable of qualitative and semi-quantitative analyses of glucosinolates in samples using data generated by ultrahigh-performance liquid chromatography-high-resolution accurate mass with multi-stage mass fragmentation (UHPLC-HRAM/MSn). A suite of bioinformatic tools was integrated into the "GLS-Finder" to perform raw data deconvolution, peak alignment, glucosinolate putative assignments, semi-quantitation, and unsupervised principal component analysis (PCA). GLS-Finder was successfully applied to identify intact glucosinolates in 49 commonly consumed Brassica vegetable samples in the United States. It is believed that this work introduces a new way of fast data processing and interpretation for qualitative and quantitative analyses of glucosinolates, where great efficacy was improved in comparison to identification manually. C1 [Sun, Jianghao; Zhang, Mengliang; Chen, Pei] USDA ARS, Beltsville Human Nutr Res Ctr, Food Composit & Methods Dev Lab, Beltsville, MD 20705 USA. RP Chen, P (reprint author), USDA ARS, Beltsville Human Nutr Res Ctr, Food Composit & Methods Dev Lab, Beltsville, MD 20705 USA. EM pei.chen@ars.usda.gov RI Zhang, Mengliang/J-3573-2015 OI Zhang, Mengliang/0000-0001-8366-0052 FU Agricultural Research Service of the United States Department of Agriculture; Interagency Agreement with the Office of Dietary Supplements at the National Institutes of Health FX This research is supported by the Agricultural Research Service of the United States Department of Agriculture and an Interagency Agreement with the Office of Dietary Supplements at the National Institutes of Health. NR 25 TC 2 Z9 2 U1 2 U2 12 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 1 PY 2016 VL 64 IS 21 BP 4407 EP 4415 DI 10.1021/acs.jafc.6b01277 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DN5ZQ UT WOS:000377150800026 PM 27181885 ER PT J AU Kingsley, DC Brown, TC AF Kingsley, David C. Brown, Thomas C. TI Endogenous and costly institutional deterrence in a public good experiment SO JOURNAL OF BEHAVIORAL AND EXPERIMENTAL ECONOMICS LA English DT Article DE Public good experiment; Central authority; Deterrence; Endogenous selection ID PUNISHMENT; ENFORCEMENT; COOPERATION; PROVISION; BEHAVIOR; CRIME; GAMES AB Modern societies rely on formal, central authority institutions that regulate the behavior of all members of society. This paper investigates the formation of a central authority regime within a linear public good experiment. The institution is funded by a fixed cost that increases with the level of deterrence, which is specified as the number of group members who are likely to be monitored. The level of deterrence is both exogenously and endogenously determined, allowing investigation of the effect of endogenous selection. The results indicate no significant positive endogenous selection effect. Indeed, in contrast to the existing literature, when a non-deterrent central authority is endogenously determined contributions tend to decrease. (C) 2016 Elsevier Inc. All rights reserved. C1 [Kingsley, David C.] Univ Massachusetts, Dept Econ, 1 Univ Ave, Lowell, MA 01854 USA. [Brown, Thomas C.] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Kingsley, DC (reprint author), Univ Massachusetts, Dept Econ, 1 Univ Ave, Lowell, MA 01854 USA. EM david_kingsley@uml.edu FU U.S. Forest Service [10-CR-11221636-255] FX We would like to thank Alexander Smith, Brock Stoddard, participants at the 2014 Economic Science Association International Meeting, and participants at the 2015 Western Massachusetts Experimental Economics Workshop. Financial support provided by the U.S. Forest Service (Grant No. 10-CR-11221636-255) is gratefully acknowledged. All errors remain our own. NR 20 TC 0 Z9 0 U1 3 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2214-8043 EI 2214-8051 J9 J BEHAV EXP ECON JI J. Behav. Exp. Econ. PD JUN PY 2016 VL 62 BP 33 EP 41 DI 10.1016/j.socec.2016.03.005 PG 9 WC Economics SC Business & Economics GA DN1EO UT WOS:000376809300004 ER PT J AU Freeland, RS Allred, BJ Martinez, LR Gamble, DL Jones, BR McCoy, EL AF Freeland, Robert S. Allred, Barry J. Martinez, Luis R. Gamble, Debra L. Jones, Brian R. McCoy, Edward L. TI Performance of Hybrid and Single-frequency Impulse GPR Antennas on USGA Sporting Greens SO JOURNAL OF ENVIRONMENTAL AND ENGINEERING GEOPHYSICS LA English DT Article ID GROUND-PENETRATING RADAR; SOIL AB The utility of employing ground-penetrating radar (GPR) technologies for environmental surveys can vary, depending upon the physical properties of the site. Environmental conditions can fluctuate, altering soil properties. Operator proficiency and survey methodology will also influence GPR findings. Therefore, GPR equipment performance evaluation involves standardized tests that are frequently conducted indoors within laboratory-controlled environments. This study uses outdoor United States Golf Association (USGA) putting greens as a structure for GPR testing for surveying practitioners. These USGA putting greens provide a tightly controlled environment because many golf courses and sports turf fields adhere to strict USGA construction and irrigation guidelines. Past studies on several USGA greens show that GPR provided precise and accurate profiles of root-mixture depth, gravel-blanket thickness, and drain-tile layout. Results are independent of locale, as all USGA putting greens are virtually identical. In this project, the performance of various GPR antenna frequencies on USGA greens was assessed. This research determined that an efficient antenna for routine profiling of turf putting greens is in the 400- to 800-MHz range. Other frequencies also performed well for a particular target or task, such as for profiling sub-grade depths. The study also compared the performance of a hybrid antenna design with the performance of fixed-frequency antennas and found that the hybrid frequencies fit well for profiling turf greens. Greens management staff may have concerns regarding damage to high-value turf from geophysical survey trafficking. Proposed surveying practices and procedures can help lessen these concerns. C1 [Freeland, Robert S.] Univ Tennessee, Dept Biosyst Engn & Soil Sci, 2506 EJ Chapman Dr, Knoxville, TN 37996 USA. [Allred, Barry J.; Martinez, Luis R.; Gamble, Debra L.] USDA ARS, Soil Drainage Res Unit, 590 Woody Hayes Dr,Room 234, Columbus, OH 43210 USA. [Jones, Brian R.] Geophys Survey Syst Inc, 12 Ind Way, Salem, NH 03079 USA. [McCoy, Edward L.] Ohio State Univ, OARDC, 1680 Madison Ave, Wooster, OH 44691 USA. RP Freeland, RS (reprint author), Univ Tennessee, Dept Biosyst Engn & Soil Sci, 2506 EJ Chapman Dr, Knoxville, TN 37996 USA.; Allred, BJ; Martinez, LR; Gamble, DL (reprint author), USDA ARS, Soil Drainage Res Unit, 590 Woody Hayes Dr,Room 234, Columbus, OH 43210 USA.; Jones, BR (reprint author), Geophys Survey Syst Inc, 12 Ind Way, Salem, NH 03079 USA.; McCoy, EL (reprint author), Ohio State Univ, OARDC, 1680 Madison Ave, Wooster, OH 44691 USA. EM rfreelan@utk.edu; barry.allred@ars.usda.gov; luis.martinez@ars.usda.gov; debra.gamble@ars.usda.gov; jonesb@geophyscial.com; mccoy.13@osu.edu NR 22 TC 0 Z9 0 U1 4 U2 4 PU ENVIRONMENTAL ENGINEERING GEOPHYSICAL SOC PI DENVER PA 1720 SOUTH BELLAIRE, STE 110, DENVER, CO 80222-433 USA SN 1083-1363 J9 J ENVIRON ENG GEOPH JI J. Environ. Eng. Geophys. PD JUN PY 2016 VL 21 IS 2 BP 57 EP 65 DI 10.2113/JEEG21.2.57 PG 9 WC Geochemistry & Geophysics; Engineering, Geological SC Geochemistry & Geophysics; Engineering GA DN5YT UT WOS:000377148300002 ER PT J AU Kocevska, D Voortman, T Dashti, HS van den Hooven, EH Ghassabian, A Rijlaarsdam, J Schneider, N Feskens, EJM Jaddoe, VWV Tiemeier, H Franco, OH AF Kocevska, Desana Voortman, Trudy Dashti, Hassan S. van den Hooven, Edith H. Ghassabian, Akhgar Rijlaarsdam, Jolien Schneider, Nora Feskens, Edith J. M. Jaddoe, Vincent W. V. Tiemeier, Henning Franco, Oscar H. TI Macronutrient Intakes in Infancy Are Associated with Sleep Duration in Toddlerhood SO JOURNAL OF NUTRITION LA English DT Article DE infant diet; macronutrients; sleep duration; awakenings; epidemiology; children ID DIETARY NUTRIENTS; EARLY-CHILDHOOD; QUALITY; ADOLESCENTS; CHILDREN; OBESITY; RISK AB Background: Dietary composition has been associated with sleep indexes. However, most of the evidence is based on cross-sectional data, and studies in young children are lacking. Objective: The aim of this study was to explore the longitudinal associations of macronutrient composition of the diet with sleep duration and consolidation (number of awakenings) in infancy and early childhood. Methods: The study was performed in 3465 children from the Generation R Study, a population-based cohort study in the Netherlands. Mothers reported their child's food intake at 13 mo of age by using a validated food-frequency questionnaire and their child's sleep patterns at 2 and 3 y of age. We used nutrient substitution models to assess the associations of relative macronutrient intakes with sleep indexes and adjusted the models for sociodemographic and lifestyle factors. Results: Isocaloric substitution of fat intake by protein or carbohydrate in infancy was associated with longer total sleep duration at 2 but not 3 y of age. For each 5% increase in energy intake of either protein or carbohydrate at the expense of fat, sleep duration at 2 y of age was longer by 6 min (95% CI: 0.4, 12 min) and 4 min (95% CI: 2, 6 min), respectively. Further exploration of macronutrient subtypes indicated no consistent differences between saturated or unsaturated fat and that intake of plant compared with animal protein or Trp did not explain the association of higher total protein intake with longer sleep duration at 2 y of age. Replacing unsaturated with saturated fat was associated with 7 min (95% CI:-13, -1 min) shorter total sleep duration at 3 y of age. Macronutrient intakes were not associated with sleep consolidation. Conclusions: Our results suggest that the macronutrient composition of the diet is associated with sleep duration in young, children. Future research should further study the causality of this association and explore the underlying mechanisms. C1 [Kocevska, Desana; Voortman, Trudy; Jaddoe, Vincent W. V.] Univ Med Ctr, Erasmus Med Ctr, Sophia Childrens Hosp, Generat R Study Grp, Rotterdam, Netherlands. [Voortman, Trudy; van den Hooven, Edith H.; Jaddoe, Vincent W. V.; Tiemeier, Henning; Franco, Oscar H.] Univ Med Ctr, Erasmus Med Ctr, Sophia Childrens Hosp, Dept Epidemiol, Rotterdam, Netherlands. [Kocevska, Desana; Ghassabian, Akhgar; Rijlaarsdam, Jolien; Tiemeier, Henning] Univ Med Ctr, Erasmus Med Ctr, Sophia Childrens Hosp, Dept Child & Adolescent Psychiat, Rotterdam, Netherlands. [Tiemeier, Henning] Univ Med Ctr, Erasmus Med Ctr, Sophia Childrens Hosp, Dept Psychiat, Rotterdam, Netherlands. [Jaddoe, Vincent W. V.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr, Nutr & Genom Lab, Boston, MA 02111 USA. [Dashti, Hassan S.] Nestec Ltd, Nestle Res Ctr, Lausanne, Switzerland. [Schneider, Nora; Feskens, Edith J. M.] Wageningen Univ, Dept Human Nutr, NL-6700 AP Wageningen, Netherlands. RP Voortman, T (reprint author), Univ Med Ctr, Erasmus Med Ctr, Sophia Childrens Hosp, Generat R Study Grp, Rotterdam, Netherlands.; Voortman, T (reprint author), Univ Med Ctr, Erasmus Med Ctr, Sophia Childrens Hosp, Dept Epidemiol, Rotterdam, Netherlands. EM trudy.voortman@erasmusmc.nl RI Voortman, Trudy/C-2963-2015; OI Voortman, Trudy/0000-0003-2830-6813; Dashti, Hassan S/0000-0002-1650-679X; Ghassabian, Akhgar/0000-0001-9551-4706 FU Erasmus Medical Center; Erasmus University; Dutch Ministry of Health, Welfare and Sport; Netherlands Organization for Health Research and Development (ZonMw); VIDI [017.106.370]; ERAWEB - European Commission [2013-2548/001-001-EMA-2]; Nestle Nutrition (Nestec Ltd.); Metagenics Inc.; AXA; ErasmusAGE FX Supported in part by the Erasmus Medical Center, the Erasmus University, the Dutch Ministry of Health, Welfare and Sport, and the Netherlands Organization for Health Research and Development (ZonMw). HT received VIDI Grant 017.106.370. The work of DK was supported by an ERAWEB scholarship grant financed by the European Commission, in accordance with the grant agreement (2013-2548/001-001-EMA-2).; Author disclosures: D Kocevska, HS Dashti, A Ghassabian, J Rijlaarsdam, EJM Feskens, VWV Jaddoe, and H Tiemeier, no conflicts of interest. T Voortman, EH van den Hooven, and OH Franco work in ErasmusAGE, a center for aging research across the life course funded by Nestle Nutrition (Nestec Ltd.), Metagenics Inc., and AXA. N Schneider works as scientist at Nestle Research Center (Nestle Ltd.). Metagenics Inc. and AXA functioned as funding sources for ErasmusAGE research center but had no role in the design and conduct of the study, data collection and analysis, or preparation or approval of the manuscript. NR 34 TC 2 Z9 2 U1 0 U2 4 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD JUN PY 2016 VL 146 IS 6 BP 1250 EP 1256 DI 10.3945/jn.115.225847 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DN4RI UT WOS:000377054500014 PM 27146923 ER PT J AU Kendra, PE Montgomery, WS Deyrup, MA Wakarchuk, D AF Kendra, Paul E. Montgomery, Wayne S. Deyrup, Mark A. Wakarchuk, David TI Improved lure for redbay ambrosia beetle developed by enrichment of alpha-copaene content SO JOURNAL OF PEST SCIENCE LA English DT Article DE alpha-Copaene; Copaiba oil; Cubeb oil; Kairomones; Laurel wilt; Xyleborus glabratus ID COLEOPTERA-CURCULIONIDAE SCOLYTINAE; SOUTHEASTERN UNITED-STATES; MEDITERRANEAN FRUIT-FLY; ESSENTIAL OIL LURES; XYLEBORUS-GLABRATUS; LAUREL WILT; RAFFAELEA-LAURICOLA; ELECTROANTENNOGRAM RESPONSES; TERPENOID EMISSIONS; BARK BEETLES AB The exotic redbay ambrosia beetle, Xyleborus glabratus Eichhoff, has become a serious invasive pest in the USA, now established in eight southeastern states. Females are the primary vectors of a fungal pathogen, Raffaelea lauricola, that causes laurel wilt, a lethal disease of trees in the family Lauraceae. Laurel wilt has caused extensive mortality in native Persea species, including redbay (P. borbonia), swampbay (P. palustris), and silkbay (P. humilis), and currently it threatens avocado (P. americana) in Florida. With continued spread, laurel wilt may impact additional Lauraceae throughout the Americas. Currently, the most effective lures for X. glabratus contain cubeb oil, an essential oil composed of a complex mixture of terpenoids. To elucidate the primary attractants of X. glabratus, fractional distillation was used to separate whole cubeb oil into 17 fractions. All fractions were used initially as substrates for electroantennographic (EAG) analyses to assess olfactory response; then eight representative fractions were chosen for binary-choice bioassays to assess behavioral response. Although fractions containing monoterpenes elicited the strongest EAG responses, significant attraction of X. glabratus was observed only with fractions that contained high percentages of the sesquiterpenes alpha-copaene and alpha-cubebene. This information was used to prepare two prototype lures, one of which contained copaiba oil (an essential oil consisting of 10 % alpha-copaene), and the other a proprietary product enriched to 50 % (-)-alpha-copaene content. In field trials, the copaiba and commercial cubeb lures captured equal numbers of X. glabratus, but the 50 % copaene lure captured significantly more beetles and had field longevity of 3 months. C1 [Kendra, Paul E.; Montgomery, Wayne S.] ARS, USDA, Subtrop Hort Res Stn, 13601 Old Cutler Rd, Miami, FL 33158 USA. [Deyrup, Mark A.] Archbold Biol Stn, POB 2057, Lake Placid, FL 33862 USA. [Wakarchuk, David] Synergy Semiochem Corp, 7061 Merritt Ave, Burnaby, BC V5J 4R7, Canada. RP Kendra, PE (reprint author), ARS, USDA, Subtrop Hort Res Stn, 13601 Old Cutler Rd, Miami, FL 33158 USA. EM paul.kendra@ars.usda.gov FU USDA-ARS National Plant Disease Recovery System; Florida Avocado Administrative Committee FX The authors are grateful to Hilary Swain for providing laboratory space and lodging at Archbold Biological Station (Lake Placid, FL), to John Smoak for granting permission to conduct field trials on his property, and to Jerome Niogret (Niogret Ecology Consulting LLC, Miami, FL), Randy Ploetz (University of Florida, Homestead), and two anonymous journal referees for providing reviews of an earlier version of the manuscript. We also acknowledge Synergy Semiochemicals Corp. (Burnaby, BC, Canada) for supplying samples for laboratory and field evaluations, and the Florida Department of Environmental Protection for granting Research/Collecting Permit #01281314 for access to Highlands Hammock State Park, Sebring, FL. This work was supported, in part, by funding from the USDA-ARS National Plant Disease Recovery System and the Florida Avocado Administrative Committee. Mention of a proprietary product does not constitute an endorsement by the USDA-ARS. NR 49 TC 4 Z9 4 U1 4 U2 10 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 EI 1612-4766 J9 J PEST SCI JI J. Pest Sci. PD JUN PY 2016 VL 89 IS 2 BP 427 EP 438 DI 10.1007/s10340-015-0708-5 PG 12 WC Entomology SC Entomology GA DN2WJ UT WOS:000376923400011 ER PT J AU Mc Donnell, R Yoo, J Patel, K Rios, L Hollingsworth, R Millar, J Paine, T AF Mc Donnell, Rory Yoo, Ju Patel, Kenna Rios, Lissette Hollingsworth, Robert Millar, Jocelyn Paine, Timothy TI Can essential oils be used as novel drench treatments for the eggs and juveniles of the pest snail Cornu aspersum in potted plants? SO JOURNAL OF PEST SCIENCE LA English DT Article DE Snails; Cornu aspersum; Potted plants; Biorational molluscicide; Essential oils; Clove bud oil ID STORED-PRODUCT INSECTS; UNITED-STATES; SPIDER-MITE; TOXICITY; MONOTERPENOIDS; COLEOPTERA; EUGENOL AB The horticultural trade is an important pathway for the introduction and spread of invasive gastropods because potted plants are essentially portable microhabitats, which protect snails and slugs, especially buried eggs and juveniles, from desiccation and molluscicides. The identification of a drench or dip treatment would therefore be an important development in helping to manage this pathway. We assessed the potential of using eleven essential oils and one terpene against the eggs and juveniles of the quarantine snail pest, Cornu aspersum. Clove bud oil was most efficacious and based on Lethal Concentration 50 (LC50) values it was 22 times more toxic than the commercially available product Snail and Slug Away(A (R)) which has cinnamon oil as its active ingredient. Importantly, at a concentration of 0.116 %, clove bud oil caused 100 % mortality of C. asperum eggs and juveniles in potted plants after 24 h and was not phytotoxic. Although more expensive than a widely used metaldehyde product (Slug-Fest All Weather Formula) clove bud oil causes rapid mortality, is pleasant smelling, is non-toxic to humans and is exempt from pesticide registration requirements and pesticide residue tolerance requirements under federal law in the United States. This exemption would decrease the time and costs associated with bringing a new molluscicide to market, which has clove bud oil as its active ingredient. C1 [Mc Donnell, Rory; Yoo, Ju; Patel, Kenna; Rios, Lissette; Millar, Jocelyn; Paine, Timothy] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Hollingsworth, Robert] USDA ARS PBARC, 64 Nowelo St, Hilo, HI 96720 USA. RP Mc Donnell, R (reprint author), Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. EM rjmcdonnell@gmail.com FU Western Region IPM Center [2007-51120-03885] FX We are grateful to the Western Region IPM Center which funded this research under grant 2007-51120-03885. NR 33 TC 0 Z9 0 U1 4 U2 7 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 EI 1612-4766 J9 J PEST SCI JI J. Pest Sci. PD JUN PY 2016 VL 89 IS 2 BP 549 EP 555 DI 10.1007/s10340-015-0690-y PG 7 WC Entomology SC Entomology GA DN2WJ UT WOS:000376923400023 ER PT J AU White, AH Maroto, ME AF White, Alicie Hogbin Maroto, Maya Edmonds TI Summer Meal Programs Provide an Opportunity for Nutrition Education and Physical Activity SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article ID SCHOOL C1 [White, Alicie Hogbin] USDA, Nutr Educ & Promot, Child Nutr Programs, Food & Nutr Serv, Alexandria, VA USA. [Maroto, Maya Edmonds] USDA, Food & Nutr Serv, Alexandria, VA USA. RP White, AH (reprint author), USDA, Nutr Educ & Promot, Child Nutr Programs, Food & Nutr Serv, Alexandria, VA USA. NR 5 TC 0 Z9 0 U1 1 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD JUN PY 2016 VL 116 IS 6 BP 905 EP 907 DI 10.1016/j.jand.2016.04.003 PG 3 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DN2GY UT WOS:000376883300003 PM 27235641 ER PT J AU Bantchev, GB Biresaw, G Palmquist, DE Murray, RE AF Bantchev, Grigor B. Biresaw, Girma Palmquist, Debra E. Murray, Rex E. TI Radical-Initiated Reaction of Methyl Linoleate with Dialkyl Phosphites SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Alkyl 9(10),12(13)-bis(dialkyl phosphono) octadecanoate; Radical addition; Radical chain reaction; Transesterification; Nonlinear least-squares data fitting; Linoleate ID PHOSPHONATES; ACID; OIL AB The addition of dialkyl phosphite (methyl, ethyl and n-butyl) to methyl linoleate (MeLin) double bonds was investigated. The reaction proved to be more challenging than the analogous reaction with methyl oleate (MeOl), due to inhibition of the radical reaction by the bis-allylic hydrogens of MeLin and the lower reactivity of MeLin double bonds. However, we demonstrated that this self-inhibition problem can be solved by simply keeping the MeLin reagent at low concentrations, while keeping the dialkyl phosphite at high concentrations. For optimization of the reaction, four different radical initiators were investigated: dilauroyl peroxide (LP), 2,2'-azobis(2-methylpropionitrile) (AIBN), tert-butyl perbenzoate (t-BP), and tert-butyl peroxide (TOOT). The initiators were used at temperatures that provided a half-life of 10 h: 64, 64, 104, and 125 A degrees C respectively for LP, AIBN, t-BP, and TOOT. The tests showed the reaction to be faster at higher temperatures, but transesterification of the ester groups was also observed at elevated temperatures. t-BP was chosen as an optimal initiator for carrying the reaction. The apparent order of reactivity of the dimethyl, diethyl and di-n-butyl phosphites (Me > Et > n-Bu) towards MeLin was due to differences in their molar volumes. When the concentrations of dialkyl phosphite were kept the same, the order reversed (n-Bu > Et-Me). GC-MS spectra of the resulting phosphonates are reported and the main fragments assigned. C1 [Bantchev, Grigor B.; Biresaw, Girma; Murray, Rex E.] ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. [Palmquist, Debra E.] ARS, Midwest Area, USDA, 1815 N Univ St, Peoria, IL 61604 USA. RP Bantchev, GB (reprint author), ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM Grigor.Bantchev@ars.usda.gov OI Bantchev, Grigor/0000-0003-2790-5195 NR 19 TC 1 Z9 1 U1 1 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD JUN PY 2016 VL 93 IS 6 BP 859 EP 868 DI 10.1007/s11746-016-2820-y PG 10 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DN3CE UT WOS:000376939200013 ER PT J AU Kumar, G Engle, C Tucker, C AF Kumar, Ganesh Engle, Carole Tucker, Craig TI Costs and Risk of Catfish Split-pond Systems SO JOURNAL OF THE WORLD AQUACULTURE SOCIETY LA English DT Article ID ICTALURUS-PUNCTATUS; CHANNEL CATFISH; HYBRID CATFISH; SALMON AQUACULTURE; STOCKING DENSITY; WATER-QUALITY; ECONOMIC RISK; STRATEGIES; FURCATUS; ADOPTION AB Split ponds are recently developed pond-based aquaculture systems that allow intensification of catfish aquaculture. Successful industry-wide adoption of newly developing technologies like split-pond systems will depend upon their productivity and cost efficiencies. Costs and production performance of the following three split-pond design scenarios were monitored in Arkansas and Mississippi: (1) research design developed at the Thad Cochran National Warmwater Aquaculture Center, Stoneville, Mississippi; (2) waterwheel design tested on commercial catfish ponds; and (3) screw-pump design tested on commercial catfish ponds. An economic engineering approach using standard enterprise budget analysis was used to develop estimates of breakeven prices (BEPs) ($/kg) for producing foodsize hybrid catfish (oIctalurus furcatusxfIctalurus punctatus) for each scenario. Estimates of BEPs of hybrid catfish raised in split ponds ranged from $1.72 to $2.05/kg. The cost of catfish production in split ponds was sensitive to yield, fish prices, and feed prices. Annual net cash flows from both commercial split-pond systems were high and sufficient to make the investment profitable in the long run. Feed price, feed conversion ratio, and yield contributed the most to downside risk of split ponds. C1 [Kumar, Ganesh] Univ Arkansas, Aquaculture & Fisheries Ctr, 1200N,Univ Dr, Pine Bluff, AR 71601 USA. [Engle, Carole] Engle Stone Aquat LLC, Strasburg, VA USA. [Tucker, Craig] Mississippi State Univ, Thad Cochran Natl Warmwater Aquaculture Ctr, USDA, ARS,Catfish Genet Res Unit, POB 38, Stoneville, MS 38776 USA. RP Kumar, G (reprint author), Univ Arkansas, Aquaculture & Fisheries Ctr, 1200N,Univ Dr, Pine Bluff, AR 71601 USA. EM ganu89@gmail.com FU Southern Regional Aquaculture Center from the United States Department of Agriculture Cooperative States Research, Education, and Extension Services [2012-38500-19665] FX The authors wish to acknowledge the review and suggestions on an earlier draft by Nathan Stone, Kelly Bryant, Luke Roy, and Mathew Recsetar. We would like to thank all the catfish producers from the states of Arkansas and Mississippi who provided us with farm data. Authors would like to thank the Southern Regional Aquaculture Center grant no. 2012-38500-19665 from the United States Department of Agriculture Cooperative States Research, Education, and Extension Services for funding this work. Mathew Recsetar, Travis Brown, Terry Hanson, and Les Torrans are acknowledged for their participation in the project. Errors or omissions are sole responsibilities of the authors. NR 39 TC 4 Z9 4 U1 4 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-8849 EI 1749-7345 J9 J WORLD AQUACULT SOC JI J. World Aquacult. Soc. PD JUN PY 2016 VL 47 IS 3 BP 327 EP 340 DI 10.1111/jwas.12271 PG 14 WC Fisheries SC Fisheries GA DN6VQ UT WOS:000377215000003 ER PT J AU Liu, ZJ Liu, SK Yao, J Bao, LS Zhang, JR Li, Y Jiang, C Sun, LY Wang, RJ Zhang, Y Zhou, T Zeng, QF Fu, Q Gao, S Li, N Koren, S Jiang, YL Zimin, A Xu, P Phillippy, AM Geng, X Song, L Sun, FY Li, C Wang, XZ Chen, AL Jin, YL Yuan, ZH Yang, YJ Tan, SX Peatman, E Lu, JG Qin, ZK Dunham, R Li, ZX Sonstegard, T Feng, JB Danzmann, RG Schroeder, S Scheffler, B Duke, MV Ballard, L Kucuktas, H Kaltenboeck, L Liu, HX Armbruster, J Xie, YJ Kirby, ML Tian, Y Flanagan, ME Mu, WJ Waldbieser, GC AF Liu, Zhanjiang Liu, Shikai Yao, Jun Bao, Lisui Zhang, Jiaren Li, Yun Jiang, Chen Sun, Luyang Wang, Ruijia Zhang, Yu Zhou, Tao Zeng, Qifan Fu, Qiang Gao, Sen Li, Ning Koren, Sergey Jiang, Yanliang Zimin, Aleksey Xu, Peng Phillippy, Adam M. Geng, Xin Song, Lin Sun, Fanyue Li, Chao Wang, Xiaozhu Chen, Ailu Jin, Yulin Yuan, Zihao Yang, Yujia Tan, Suxu Peatman, Eric Lu, Jianguo Qin, Zhenkui Dunham, Rex Li, Zhaoxia Sonstegard, Tad Feng, Jianbin Danzmann, Roy G. Schroeder, Steven Scheffler, Brian Duke, Mary V. Ballard, Linda Kucuktas, Huseyin Kaltenboeck, Ludmilla Liu, Haixia Armbruster, Jonathan Xie, Yangjie Kirby, Mona L. Tian, Yi Flanagan, Mary Elizabeth Mu, Weijie Waldbieser, Geoffrey C. TI The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts SO NATURE COMMUNICATIONS LA English DT Article ID SECRETORY CALCIUM-BINDING; SCPP GENE EVOLUTION; ICTALURUS-PUNCTATUS; TISSUE MINERALIZATION; PHYSICAL MAP; HIGH-DENSITY; ZEBRAFISH; FISH; DUPLICATION; RECEPTOR AB Catfish represent 12% of teleost or 6.3% of all vertebrate species, and are of enormous economic value. Here we report a high-quality reference genome sequence of channel catfish (Ictalurus punctatus), the major aquaculture species in the US. The reference genome sequence was validated by genetic mapping of 54,000 SNPs, and annotated with 26,661 predicted protein-coding genes. Through comparative analysis of genomes and transcriptomes of scaled and scaleless fish and scale regeneration experiments, we address the genomic basis for the most striking physical characteristic of catfish, the evolutionary loss of scales and provide evidence that lack of secretory calcium-binding phosphoproteins accounts for the evolutionary loss of scales in catfish. The channel catfish reference genome sequence, along with two additional genome sequences and transcriptomes of scaled catfishes, provide crucial resources for evolutionary and biological studies. This work also demonstrates the power of comparative subtraction of candidate genes for traits of structural significance. C1 [Liu, Zhanjiang; Liu, Shikai; Yao, Jun; Bao, Lisui; Zhang, Jiaren; Li, Yun; Jiang, Chen; Sun, Luyang; Wang, Ruijia; Zhang, Yu; Zhou, Tao; Zeng, Qifan; Fu, Qiang; Gao, Sen; Li, Ning; Jiang, Yanliang; Xu, Peng; Geng, Xin; Song, Lin; Sun, Fanyue; Li, Chao; Wang, Xiaozhu; Chen, Ailu; Jin, Yulin; Yuan, Zihao; Yang, Yujia; Tan, Suxu; Peatman, Eric; Lu, Jianguo; Qin, Zhenkui; Dunham, Rex; Li, Zhaoxia; Feng, Jianbin; Kucuktas, Huseyin; Kaltenboeck, Ludmilla; Liu, Haixia; Xie, Yangjie; Tian, Yi; Mu, Weijie] Auburn Univ, Fish Mol Genet & Biotechnol Lab, Sch Fisheries Aquaculture & Aquat Sci, Auburn, AL 36849 USA. [Liu, Zhanjiang; Liu, Shikai; Yao, Jun; Bao, Lisui; Zhang, Jiaren; Li, Yun; Jiang, Chen; Sun, Luyang; Wang, Ruijia; Zhang, Yu; Zhou, Tao; Zeng, Qifan; Fu, Qiang; Gao, Sen; Li, Ning; Jiang, Yanliang; Xu, Peng; Geng, Xin; Song, Lin; Sun, Fanyue; Li, Chao; Wang, Xiaozhu; Chen, Ailu; Jin, Yulin; Yuan, Zihao; Yang, Yujia; Tan, Suxu; Peatman, Eric; Lu, Jianguo; Qin, Zhenkui; Dunham, Rex; Li, Zhaoxia; Feng, Jianbin; Kucuktas, Huseyin; Kaltenboeck, Ludmilla; Liu, Haixia; Xie, Yangjie; Tian, Yi; Mu, Weijie] Auburn Univ, Program Cell & Mol Biosci, Auburn, AL 36849 USA. [Koren, Sergey; Phillippy, Adam M.] Natl Ctr Biodefense Anal, 110 Thomas Johnson Dr, Ft Detrick, MD 21702 USA. [Koren, Sergey; Phillippy, Adam M.] Countermeasures Ctr, 110 Thomas Johnson Dr, Ft Detrick, MD 21702 USA. [Zimin, Aleksey] Univ Maryland, Inst Phys Sci & Technol, College Pk, MD 20742 USA. [Sonstegard, Tad; Schroeder, Steven] ARS, Bovine Funct Genom Lab, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. [Danzmann, Roy G.] Univ Guelph, Dept Integrat Biol, Guelph, ON N1G 2W1, Canada. [Scheffler, Brian; Duke, Mary V.; Ballard, Linda] ARS, USDA, Genom & Bioinformat Res Unit, POB 38, Stoneville, MS 38776 USA. [Armbruster, Jonathan] Auburn Univ, Dept Biol Sci, Auburn, AL 36849 USA. [Kirby, Mona L.; Flanagan, Mary Elizabeth; Waldbieser, Geoffrey C.] USDA ARS Warmwater Aquaculture Res Unit, POB 38,141 Expt Stn Rd, Stoneville, MS 38776 USA. [Koren, Sergey; Phillippy, Adam M.] NHGRI, NIH, Bethesda, MD 20892 USA. [Sonstegard, Tad] Recombinetics Inc, 1246 Univ Ave W 301, St Paul, MN 55104 USA. RP Liu, ZJ (reprint author), Auburn Univ, Fish Mol Genet & Biotechnol Lab, Sch Fisheries Aquaculture & Aquat Sci, Auburn, AL 36849 USA.; Liu, ZJ (reprint author), Auburn Univ, Program Cell & Mol Biosci, Auburn, AL 36849 USA. EM liuzhan@auburn.edu RI Xu, Peng/J-4018-2012; OI Schroeder, Steven/0000-0001-9103-5150; Scheffler, Brian/0000-0003-1968-8952 FU Agriculture and Food Research Initiative (AFRI) [2009-35205-05101, 2010-65205-20356, 2012-67015-19410]; USDA National Institute of Food and Agriculture (NIFA) Animal Genomics, Genetics, and Breeding Program [2015-67015-22907]; USDA Agricultural Research Service [CRIS 6402-31000-009-00D]; Department of Homeland Security Science and Technology Directorate (DHS/ST) [HSHQDC-07-C-00020] FX This project was supported by Agriculture and Food Research Initiative (AFRI) Competitive Grants 2009-35205-05101, 2010-65205-20356 and 2012-67015-19410, and partially by Competitive Grant no. 2015-67015-22907 from the USDA National Institute of Food and Agriculture (NIFA) Animal Genomics, Genetics, and Breeding Program and CRIS 6402-31000-009-00D of the USDA Agricultural Research Service. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. The contributions of SK and AMP were funded under Agreement No. HSHQDC-07-C-00020 awarded by the Department of Homeland Security Science and Technology Directorate (DHS/S&T) for the management and operation of the National Biodefense Analysis and Countermeasures Center (NBACC), a Federally Funded Research and Development Center. The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the U.S. Department of Homeland Security. In no event shall the DHS, NBACC or Battelle National Biodefense Institute (BNBI) have any responsibility or liability for any use, misuse, inability to use or reliance on the information contained herein. The Department of Homeland Security does not endorse any products or commercial services mentioned in this publication. We thank Renee Beam and Karen Veverica for their support in production and maintenance of experimental fish, and to thank Brian Walenz, Jason Miller and Steven Salzberg for their discussions regarding genome assembly. NR 68 TC 16 Z9 16 U1 13 U2 33 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD JUN PY 2016 VL 7 AR 11757 DI 10.1038/ncomms11757 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DN5XC UT WOS:000377144000001 PM 27249958 ER PT J AU Dumlupinar, Z Brown, R Campbell, R Jellen, EN Anderson, J Bonman, JM Carson, M Chao, S Obert, D Jackson, E AF Dumlupinar, Ziya Brown, Ryan Campbell, Robert Jellen, Eric N. Anderson, Joe Bonman, J. Michael Carson, Martin Chao, Shiaoman Obert, Don Jackson, Eric TI The art of attrition: development of robust oat microsatellites SO PLANT BREEDING LA English DT Article DE oat; Avena sativa; microsatellites; SSRs ID SIMPLE SEQUENCE REPEAT; PCR-BASED MARKERS; BETA-GLUCAN; GENETIC DIVERSITY; LINKAGE MAP; HYPERCHOLESTEROLEMIC SUBJECTS; ALPHA-TOCOPHEROL; DART MARKERS; DNA MARKERS; AVENA SPP. AB Microsatellite or simple sequence repeat (SSR) markers are important tools for genetic analyses, especially those targeting diversity. The primary objective of this study was to develop robust oat-based microsatellite markers from newly enriched genomic libraries to expand on a relatively small existing oat SSR toolbox. Microsatellite motifs characterized by (CA/GT), (AAT/TTA), (ATG/TAC) and (CATC/GTAG) repeats were targeted for enrichment. Preliminary screening showed that 90% of clones from the (CA/GT) and 79% of the clones from the (ATG/TAC) libraries contained repeats, while < 11% of the clones from (AAT/TTA) and (CATC/GTAG) libraries contained repeats. Subsequent sequencing of 1536 clones from the (CA/GT) and (ATG/TAC) libraries resulted in 539 and 578 SSRs for which primers were designed, respectively. A total of 246 SSRs were polymorphic across 11 oat lines. One hundred and twenty-five of the markers produced highly reproducible assays that interrogated 369 alleles at 193 loci. Of these, 79 robust assays interrogated 146 codominant alleles. These markers will be useful for a wide range of genetic analyses in oat including assessment of diversity and marker-assisted breeding. C1 [Dumlupinar, Ziya] Kahramanmaras Sutcu Imam Univ, Dept Agr Biotechnol, Kahramanmaras, Turkey. [Brown, Ryan; Jackson, Eric] Gen Mills Crop Biosci, 150 North Res Campus Dr, Kannapolis, NC 28081 USA. [Campbell, Robert; Bonman, J. Michael] ARS, USDA, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. [Jellen, Eric N.] Brigham Young Univ, Dept Plant & Wildlife Sci, Provo, UT 84602 USA. [Anderson, Joe] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. [Carson, Martin] ARS, USDA, Cereal Dis Lab, St Paul, MN 55108 USA. [Chao, Shiaoman] ARS, USDA, Cereal Crops Res, Fargo, ND 58105 USA. [Obert, Don] Limagrain Cereal Seeds, 9020 Grant Rd, Battle Ground, IN 47920 USA. RP Dumlupinar, Z (reprint author), Kahramanmaras Sutcu Imam Univ, Dept Agr Biotechnol, Kahramanmaras, Turkey. EM zdumlupinar@ksu.edu.tr NR 50 TC 0 Z9 0 U1 4 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 EI 1439-0523 J9 PLANT BREEDING JI Plant Breed. PD JUN PY 2016 VL 135 IS 3 BP 323 EP 334 DI 10.1111/pbr.12362 PG 12 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA DN6YZ UT WOS:000377223800008 ER PT J AU Ma, JB Shi, AN Mou, BQ Evans, M Clark, JR Motes, D Correll, JC Xiong, HZ Qin, J Chitwood, J Weng, YJ AF Ma, Jianbing Shi, Ainong Mou, Beiquan Evans, Michael Clark, John R. Motes, Dennis Correll, Jim C. Xiong, Haizheng Qin, Jun Chitwood, Jessica Weng, Yuejin TI Association mapping of leaf traits in spinach (Spinacia oleracea L.) SO PLANT BREEDING LA English DT Article DE spinach; Spinacia oleracea; association mapping; single nucleotide polymorphism; genotyping-by-sequencing; leaf surface texture; petiole colour; edge shape ID GENOME-WIDE ASSOCIATION; LINKAGE DISEQUILIBRIUM; COMPLEX TRAITS; GENETIC DIVERSITY; MARKERS; POPULATION; PLANTS; SOFTWARE; DISSECT; MAIZE AB Spinach (Spinacia oleracea L.) is an important leafy vegetable crop grown worldwide. Leaf traits, surface texture (smooth, savoy or semi-savoy), petiole colour (different shades of green vs. purple) and edge shape (serrate vs. entire), are important commercial traits of spinach. Association mapping for the three traits was conducted on 323 USDA spinach germplasm accessions, originally collected from 33 countries and representing the entire USDA spinach germplasm collection. The majority of accessions were from Europe (36.3%), Asia (25.3%) and North America (15.8%). The majority of the spinach accessions (82.0%) were smooth (unwrinkled types), whereas the savoy and semi-savoy types (wrinkled types) accounted for 18.0%. The collection contained 74.9% green petiole types, while the purple petioles consisted of 25.1%. The collection consisted of 27.2% serrated leaf types and 72.8% entire leaf edge types. Genotyping-by-sequencing (GBS) was used for single nucleotide polymorphism (SNP) discovery, and SNPs were used as genotypic data to conduct genetic diversity and association mapping of the three leaf traits. Five genetic subpopulations and principal components (PCs) were postulated by structure 2 and JMP Genomics 7 for this association panel. Five, seven and 14 SNPs were identified to be associated with surface texture, edge shape and petiole colour, respectively. This study provides us an approach to identify SNP markers through association analysis in spinach and thus leads to select these three leaf traits through marker-assisted selection in spinach breeding programme. C1 [Ma, Jianbing; Shi, Ainong; Evans, Michael; Clark, John R.; Xiong, Haizheng; Qin, Jun; Chitwood, Jessica; Weng, Yuejin] Univ Arkansas, Dept Hort, Fayetteville, AR 72701 USA. [Mou, Beiquan] ARS, Crop Improvement & Protect Res Unit, USDA, Salinas, CA 93905 USA. [Motes, Dennis] Univ Arkansas, Vegetable Res Ctr, Alma, AR 72921 USA. [Correll, Jim C.] Univ Arkansas, Dept Plant Pathol, Fayetteville, AR 72701 USA. RP Shi, AN (reprint author), Univ Arkansas, Dept Hort, Fayetteville, AR 72701 USA.; Mou, BQ (reprint author), ARS, Crop Improvement & Protect Res Unit, USDA, Salinas, CA 93905 USA. EM ashi@uark.edu; beiquan.mou@ars.usda.gov FU USDA National Institute of Food and Agriculture Hatch Project [1002423] FX This work was supported, in part, by the USDA National Institute of Food and Agriculture Hatch Project accession number 1002423. NR 36 TC 0 Z9 0 U1 9 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 EI 1439-0523 J9 PLANT BREEDING JI Plant Breed. PD JUN PY 2016 VL 135 IS 3 BP 399 EP 404 DI 10.1111/pbr.12369 PG 6 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA DN6YZ UT WOS:000377223800017 ER PT J AU Drolet, BS Reister-Hendricks, LM Podell, BK Breitenbach, JE McVey, DS van Rijn, PA Bowen, RA AF Drolet, Barbara S. Reister-Hendricks, Lindsey M. Podell, Brendan K. Breitenbach, Jonathan E. McVey, D. Scott van Rijn, Piet A. Bowen, Richard A. TI European Bluetongue Serotype 8: Disease Threat Assessment for US Sheep SO VECTOR-BORNE AND ZOONOTIC DISEASES LA English DT Article DE Bluetongue virus; BTV-8; Orbivirus; Sheep; US ID POLYMERASE CHAIN-REACTION; CLINICAL SIGNS; FIELD OBSERVATIONS; VIRUS; INFECTION; CATTLE; NETHERLANDS; RESPONSES; EPIDEMIC AB Bluetongue virus (BTV) is an orbivirus transmitted by biting midges (Culicoides spp.) that can result in moderate to high morbidity and mortality primarily in sheep and white-tailed deer. Although only 5 serotypes of BTV are considered endemic to the United States, as many as 11 incursive serotypes have been detected in livestock and wildlife in the past 16 years. Introductions of serotypes, with unknown virulence and disease risk, are constant threats to US agriculture. One potential incursive serotype of particular concern is the European strain of BTV-8, which was introduced into Northern Europe in 2006 and caused unprecedented livestock disease and mortality during the 2006-2007 vector seasons. To assess disease risk of BTV-8 in a common white-faced American sheep breed, eight Polled Dorset yearlings were experimentally infected and monitored for clinical signs. Viremia and viral tissue distribution were detected and quantified by real-time qRT-PCR. Overall, clinical disease was moderate with no mortality. Viremia reached as high as 9.7 log10 particles/mL and persisted at 5 logs or higher through the end of the study (28 days). Virus distribution in tissues was extensive with the highest mean titers at the peak of viremia (day 8) in the kidney (8.38 log10 particles/mg) and pancreas (8.37 log10 particles/mg). Virus persisted in tissues of some sheep at 8 logs or higher by day 28. Results of this study suggest that should BTV-8 emerge in the United States, clinical disease in this common sheep breed would likely be similar in form, duration, and severity to what is typically observed in severe outbreaks of endemic serotypes, not the extraordinary disease levels seen in Northern Europe. In addition, a majority of exposed sheep would be expected to survive and act as significant BTV-8 reservoirs with high titer viremias for subsequent transmission to other livestock and wildlife populations. C1 [Drolet, Barbara S.; Reister-Hendricks, Lindsey M.; Breitenbach, Jonathan E.; McVey, D. Scott] ARS, Arthropod Borne Anim Dis Res Unit, USDA, 1515 Coll Ave, Manhattan, KS 66535 USA. [Podell, Brendan K.; Bowen, Richard A.] Colorado State Univ, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA. [van Rijn, Piet A.] Wageningen Univ, Dept Virol, Cent Vet Inst, Lelystad, Netherlands. [van Rijn, Piet A.] North West Univ, Dept Biochem, Ctr Human Metabon, Potchefstroom, South Africa. RP Drolet, BS (reprint author), ARS, Arthropod Borne Anim Dis Res Unit, USDA, 1515 Coll Ave, Manhattan, KS 66535 USA. EM barbara.drolet@ars.usda.gov RI van Rijn, Piet/C-1163-2017; Podell, Brendan/D-8396-2017 OI van Rijn, Piet/0000-0002-2594-1232; Podell, Brendan/0000-0003-2548-8826 FU USDA, ARS, Animal Health National Program [NP103, 5430-32000-006] FX Funding for this research was provided by the USDA, ARS, NP103 Animal Health National Program, project number 5430-32000-006. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. NR 32 TC 0 Z9 0 U1 0 U2 2 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1530-3667 EI 1557-7759 J9 VECTOR-BORNE ZOONOT JI Vector-Borne Zoonotic Dis. PD JUN PY 2016 VL 16 IS 6 BP 400 EP 407 DI 10.1089/vbz.2015.1924 PG 8 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA DN4DP UT WOS:000377013500006 PM 27111674 ER PT J AU Yang, CB Yang, XJ Lackeyram, D Rideout, TC Wang, ZR Stoll, B Yin, YL Burrin, DG Fan, MZ AF Yang, Chengbo Yang, Xiaojian Lackeyram, Dale Rideout, Todd C. Wang, Zirong Stoll, Barbara Yin, Yulong Burrin, Douglas G. Fan, Ming Z. TI Expression of apical Na+-L-glutamine co-transport activity, B-0-system neutral amino acid co-transporter (B(0)AT1) and angiotensin-converting enzyme 2 along the jejunal crypt-villus axis in young pigs fed a liquid formula SO AMINO ACIDS LA English DT Article DE Small intestinal apical L-glutamine uptake; System-B-0 Na+-neutral amino acid co-transporter; Gut crypt-villus axis; Angiotensin-converting enzyme 2; SLC6A19 ID BRUSH-BORDER MEMBRANE; SMALL-INTESTINE; HARTNUP DISORDER; FUNCTIONAL-CHARACTERIZATION; ONTOGENIC DEVELOPMENT; TRANSPORT-SYSTEMS; NUTRIENT TRANSPORTERS; POSTNATAL-DEVELOPMENT; PROLINE TRANSPORTER; MOLECULAR-CLONING AB Gut apical amino acid (AA) transport activity is high at birth and during suckling, thus being essential to maintain luminal nutrient-dependent mucosal growth through providing AA as essential metabolic fuel, substrates and nutrient stimuli for cellular growth. Because system-B-0 Na+-neutral AA co-transporter (B(0)AT1, encoded by the SLC6A19 gene) plays a dominant role for apical uptake of large neutral AA including l-Gln, we hypothesized that high apical Na+-Gln co-transport activity, and B(0)AT1 (SLC6A19) in co-expression with angiotensin-converting enzyme 2 (ACE2) were expressed along the entire small intestinal crypt-villus axis in young animals via unique control mechanisms. Kinetics of Na+-Gln co-transport activity in the apical membrane vesicles, prepared from epithelial cells sequentially isolated along the jejunal crypt-villus axis from liquid formula-fed young pigs, were measured with the membrane potential being clamped to zero using thiocyanate. Apical maximal Na+-Gln co-transport activity was much higher (p < 0.05) in the upper villus cells than in the middle villus (by 29 %) and the crypt (by 30 %) cells, whereas Na+-Gln co-transport affinity was lower (p < 0.05) in the upper villus cells than in the middle villus and the crypt cells. The B(0)AT1 (SLC6A19) mRNA abundance was lower (p < 0.05) in the crypt (by 40-47 %) than in the villus cells. There were no significant differences in B(0)AT1 and ACE2 protein abundances on the apical membrane among the upper villus, the middle villus and the crypt cells. Our study suggests that piglet fast growth is associated with very high intestinal apical Na+-neutral AA uptake activities via abundantly co-expressing B(0)AT1 and ACE2 proteins in the apical membrane and by transcribing the B(0)AT1 (SLC6A19) gene in the epithelia along the entire crypt-villus axis. C1 [Yang, Chengbo; Yang, Xiaojian; Lackeyram, Dale; Rideout, Todd C.; Fan, Ming Z.] Univ Guelph, Dept Anim Biosci, Guelph, ON N1G 2W1, Canada. [Yang, Chengbo] Univ Manitoba, Fac Agr & Food Sci, Dept Anim Sci, Winnipeg, MB R3T 2N2, Canada. [Yang, Xiaojian] Univ Minnesota, Southern Res & Outreach Ctr, Waseca, MN 56093 USA. [Rideout, Todd C.] SUNY Buffalo, Dept Exercise & Nutr Sci, New York, NY 14214 USA. [Wang, Zirong] Xinjiang Agr Univ, Coll Anim Sci, Urumqi 830052, Xinjiang, Peoples R China. [Stoll, Barbara; Burrin, Douglas G.] Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, USDA,ARS, Houston, TX 77030 USA. [Yin, Yulong] Chinese Acad Sci, Inst Subtrop Agr, Changsha 410125, Hunan, Peoples R China. RP Yang, CB; Fan, MZ (reprint author), Univ Guelph, Dept Anim Biosci, Guelph, ON N1G 2W1, Canada. EM chengbo.yang@umanitoba.ca; mfan@uoguelph.ca FU Natural Sciences and Engineering Research Council (NSERC) of Canada; Ontario Ministry of Agriculture, Food and Rural Affairs (OMAFRA) - University of Guelph Partnership's Animal Research Program; USDA/ARS [58-6250-6-001, R01-HD33920]; Baylor College of Medicine, Houston, TX; Chinese Academy of Sciences; Chinese Ministry of Education FX This work was supported by research grants from the Natural Sciences and Engineering Research Council (NSERC) of Canada - Discovery Program and the Ontario Ministry of Agriculture, Food and Rural Affairs (OMAFRA) - University of Guelph Partnership's Animal Research Program (to M.Z. Fan). This work was also partially supported by research grants from USDA/ARS under Cooperative Agreements 58-6250-6-001/the National Institute of Health R01-HD33920 (to D.G. Burrin). Fan M.Z. was supported by a USDA/ARS postdoctoral research fellowship when the project was initiated at the Baylor College of Medicine, Houston, TX. Yang C. was supported by a visiting scholarship from the Chinese Academy of Sciences and Wang Z. was supported by a visiting scholarship from the Chinese Ministry of Education while participating in related research activities in Guelph, ON, Canada. We are grateful to Professor Francois Verrey from the University of Zurich to provide the rabbit-anti-mouse B0AT1 for this research. NR 80 TC 1 Z9 1 U1 2 U2 3 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0939-4451 EI 1438-2199 J9 AMINO ACIDS JI Amino Acids PD JUN PY 2016 VL 48 IS 6 BP 1491 EP 1508 DI 10.1007/s00726-016-2210-7 PG 18 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA DM8JY UT WOS:000376609900014 PM 26984322 ER PT J AU Melo, AMA Alexandre, DL Furtado, RF Borges, MF Figueiredo, EAT Biswas, A Cheng, HN Alves, CR AF Araujo Melo, Airis Maria Alexandre, Dalila L. Furtado, Roselayne F. Borges, Maria F. Figueiredo, Evania Altina T. Biswas, Atanu Cheng, Huai N. Alves, Carlucio R. TI Electrochemical immunosensors for Salmonella detection in food SO APPLIED MICROBIOLOGY AND BIOTECHNOLOGY LA English DT Review DE Salmonella; Immunosensor; Food safety; Pathogen; Rapid detection ID IMPEDIMETRIC IMMUNOSENSOR; PIEZOELECTRIC IMMUNOSENSOR; PATHOGEN DETECTION; PROTEIN-A; ANTIBODY IMMOBILIZATION; CAPACITIVE IMMUNOSENSOR; GOLD NANOPARTICLES; RAPID DETECTION; BIOSENSORS; TYPHIMURIUM AB Pathogen detection is a critical point for the identification and the prevention of problems related to food safety. Failures at detecting contaminations in food may cause outbreaks with drastic consequences to public health. In spite of the real need for obtaining analytical results in the shortest time possible, conventional methods may take several days to produce a diagnosis. Salmonella spp. is the major cause of foodborne diseases worldwide and its absence is a requirement of the health authorities. Biosensors are bioelectronic devices, comprising bioreceptor molecules and transducer elements, able to detect analytes (chemical and/or biological species) rapidly and quantitatively. Electrochemical immunosensors use antibody molecules as bioreceptors and an electrochemical transducer. These devices have been widely used for pathogen detection at low cost. There are four main techniques for electrochemical immunosensors: amperometric, impedimetric, conductometric, and potentiometric. Almost all types of immunosensors are applicable to Salmonella detection. This article reviews the developments and the applications of electrochemical immunosensors for Salmonella detection, particularly the advantages of each specific technique. Immunosensors serve as exciting alternatives to conventional methods, allowing "real-time" and multiple analyses that are essential characteristics for pathogen detection and much desired in health and safety control in the food industry. C1 [Araujo Melo, Airis Maria; Alexandre, Dalila L.; Alves, Carlucio R.] Univ Estadual Ceara, Dept Chem, Ave Paranjana 1700, BR-60740903 Fortaleza, Ceara, Brazil. [Furtado, Roselayne F.; Borges, Maria F.] Embrapa Trop Agroind, Rua Dra Sara Mesquita 2270, BR-60511110 Fortaleza, Ceara, Brazil. [Figueiredo, Evania Altina T.] Univ Fed Ceara, Dept Food Sci & Technol, Campus Pici,Bloco 858, Fortaleza, Ceara, Brazil. [Biswas, Atanu] ARS, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. [Cheng, Huai N.] ARS, USDA, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. RP Melo, AMA (reprint author), Univ Estadual Ceara, Dept Chem, Ave Paranjana 1700, BR-60740903 Fortaleza, Ceara, Brazil. EM airismelo@oi.com.br FU CNPq [475174/2012-7]; CAPES FX The authors acknowledge the financial support of the CNPq (Award number: 475174/2012-7) and CAPES Brazilian agencies. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 90 TC 0 Z9 0 U1 38 U2 55 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0175-7598 EI 1432-0614 J9 APPL MICROBIOL BIOT JI Appl. Microbiol. Biotechnol. PD JUN PY 2016 VL 100 IS 12 BP 5301 EP 5312 DI 10.1007/s00253-016-7548-y PG 12 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DM6JB UT WOS:000376456700009 PM 27138197 ER PT J AU Liu, X Lu, YF Guan, XF Zhao, MK Wang, J Li, F AF Liu, Xing Lu, Yuan-Fu Guan, Xinfu Zhao, Mingkun Wang, Jin Li, Feng TI Characterizing novel metabolic pathways of melatonin receptor agonist agomelatine using metabolomic approaches SO BIOCHEMICAL PHARMACOLOGY LA English DT Article DE Agomelatine; Melatonin-receptor; Metabolism; Reactive metabolites; Metabolomics ID MAJOR DEPRESSIVE DISORDER; REACTIVE METABOLITES; DRUG DISCOVERY; ANTIDEPRESSANT; BIOACTIVATION; PERSPECTIVE; MECHANISMS; BROMOBENZENE; TOXICITY; EFFICACY AB Agomelatine (AGM), an analog of melatonin, is a potential agonist at melatonin receptors 1/2 and a selective antagonist at 5-hydroxytryptamine 2C receptors. AGM is widely used for the treatment of major depressive episodes in adults. However, multiple adverse effects associated with AGM have been reported in clinical practice. It is little known about AGM metabolism in vitro and in vivo, although metabolism plays a pivotal role in its efficacy and safety. To elucidate metabolic pathways of AGM, we systemically investigated AGM metabolism and its bioactivation in human liver microsomes (HLM) and mice using metabolomic approaches. We identified thirty-eight AGM metabolites and adducts, among which thirty-two are novel. In HLM, we uncovered five GSH-trapped adducts and two semicarbazide-trapped aldehydes. Moreover, we characterized three N-acetyl cysteine conjugated-AGM adducts in mouse urine and feces, which were formed from the degradation of AGM_GSH adducts. Using recombinant CYP450 isoenzymes and chemical inhibitors, we demonstrated that CYP1A2 and CYP3A4 are primary enzymes contributing to the formation of AGM_GSH adducts and AGM_hydrazones. This study provided a global view of AGM metabolism and identified the novel pathways of AGM bioactivation, which could be utilized for further understanding the mechanism of adverse effects related to AGM and possible drug-drug interactions. (C) 2016 Elsevier Inc. All rights reserved. C1 [Liu, Xing; Li, Feng] Baylor Coll Med, Alkek Ctr Mol Discovery, Dept Mol & Cellular Biol, Adv Technol Core, Houston, TX 77030 USA. [Lu, Yuan-Fu] Zunyi Med Coll, Key Lab Basic Pharmacol, Minist Educ, Zunyi 563000, Guizhou, Peoples R China. [Guan, Xinfu] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, DC 77030 USA. [Zhao, Mingkun; Wang, Jin] Baylor Coll Med, Dept Pharmacol, Houston, DC 77030 USA. [Zhao, Mingkun] Baylor Coll Med, Grad Program Integrat Mol & Biomed Sci, Houston, DC 77030 USA. RP Li, F (reprint author), Baylor Coll Med, Alkek Ctr Mol Discovery, Dept Mol & Cellular Biol, Adv Technol Core, Houston, TX 77030 USA. EM fl3@bcm.edu OI Li, Feng /0000-0002-9680-4614; Wang, Jin/0000-0003-3625-7919 FU Alkek Center for Molecular Discovery and Metabolomics core facility; National Institutes of Health [R01-GM115622]; Welch Foundation [Q-1798]; Cancer Prevention and Research Institute of Texas [CPRIT R1104] FX We are grateful for the fund from Alkek Center for Molecular Discovery and Metabolomics core facility, the National Institutes of Health (R01-GM115622 to J.W.), the Welch Foundation (Q-1798 to J.W.), Cancer Prevention and Research Institute of Texas (CPRIT R1104 to J.W.). NR 38 TC 1 Z9 1 U1 3 U2 8 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0006-2952 EI 1873-2968 J9 BIOCHEM PHARMACOL JI Biochem. Pharmacol. PD JUN 1 PY 2016 VL 109 BP 70 EP 82 DI 10.1016/j.bcp.2016.03.020 PG 13 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA DM5VW UT WOS:000376419400006 PM 27021842 ER PT J AU Meyer, SE Leger, EA Eldon, DR Coleman, CE AF Meyer, Susan E. Leger, Elizabeth A. Eldon, Desiree R. Coleman, Craig E. TI Strong genetic differentiation in the invasive annual grass Bromus tectorum across the Mojave-Great Basin ecological transition zone SO BIOLOGICAL INVASIONS LA English DT Article DE Cheatgrass; Climate change; Downy brome; Ecological genetics; Ecotone; Invasive species; Pre-adaptation; SNP (single nucleotide polymorphism) ID INBREEDING ANNUAL GRASS; CLIMATIC NICHE SHIFTS; SEED SIZE; PHENOTYPIC PLASTICITY; SENECIO-INAEQUIDENS; NORTH-AMERICA; CHEATGRASS; COLONIZATION; POPULATIONS; RANGE AB Bromus tectorum, an inbreeding annual grass, is a dominant invader in sagebrush steppe habitat in North America. It is also common in warm and salt deserts, displaying a larger environmental tolerance than most native species. We tested the hypothesis that a suite of habitat-specific B. tectorum lineages dominates warm desert habitats. We sampled 30 B. tectorum Mojave Desert and desert fringe populations and genotyped 10-26 individuals per population using 69 single nucleotide polymorphic (SNP) markers. We compared these populations to 11 Great Basin steppe and salt desert populations. Populations from warm desert habitats were dominated by members of two haplogroups (87 % of individuals) that were distinct from haplogroups common in Great Basin habitats. We conducted common garden studies comparing adaptive traits and field performance among haplogroups typically found in different habitats. In contrast to the haplogroup abundant in sagebrush steppe, warm desert haplogroups generally lacked a vernalization requirement for flowering. The most widespread warm desert haplogroup (Warm Desert 1) also had larger seeds and a higher root: shoot ratio than other haplogroups. In the field, performance of warm desert haplogroups was dramatically lower than the sagebrush steppe haplogroup at one steppe site, but one warm desert haplogroup performed as well as the steppe haplogroup under drought conditions at the other site. Our results suggest that B. tectorum succeeds in widely disparate environments through ecotypic variation displayed by distinct lineages of plants. Accounting for this ecotypic variation is essential in modeling its future distribution in response to climate change. C1 [Meyer, Susan E.] USFS Rocky Mt Res Stn, Shrub Sci Lab, 735 North 500 East, Provo, UT 84606 USA. [Leger, Elizabeth A.] Univ Nevada, Dept Nat Resources & Environm Sci, 1664 N Virginia St,MS 186, Reno, NV 89557 USA. [Eldon, Desiree R.; Coleman, Craig E.] Brigham Young Univ, Dept Plant & Wildlife Sci, Provo, UT 84602 USA. RP Meyer, SE (reprint author), USFS Rocky Mt Res Stn, Shrub Sci Lab, 735 North 500 East, Provo, UT 84606 USA. EM semeyer@xmission.com FU USDA Cooperative State Research Service [2008-35320-18677]; Joint Fire Sciences Program [JFSP-2007-1-3-10, JFSP-2011-S-2-6]; Bureau of Land Management (Integrated Cheatgrass Die-off Research Project); University of Nevada Reno (start-up funds) FX This work was supported in part by grants from the USDA Cooperative State Research Service (2008-35320-18677 to S.E.M. and C.E.C.), the Joint Fire Sciences Program (JFSP-2007-1-3-10, JFSP-2011-S-2-6 to S.E.M.), the Bureau of Land Management (Integrated Cheatgrass Die-off Research Project to S.E.M. and E.A.L.), and the University of Nevada Reno (start-up funds to E.A.L.). Thanks to Phil Allen, Joshua Nicholson, David Salman, and Bettina Schultz for seed collection, to Keith Merrill and Sam Decker for help with the SNP analysis, to Suzette Clement and Joshua Nicholson for assistance with the field study in Utah, to Sandra Li, Owen Baughman, Bryce Wehan, and Erin Goergen for field and greenhouse assistance in Nevada, and to Bettina Schultz for preparing the color graphics. NR 52 TC 1 Z9 1 U1 16 U2 28 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD JUN PY 2016 VL 18 IS 6 BP 1611 EP 1628 DI 10.1007/s10530-016-1105-6 PG 18 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DM9CZ UT WOS:000376663400008 ER PT J AU Anandhi, A Steiner, JL Bailey, N AF Anandhi, Aavudai Steiner, Jean L. Bailey, Nathaniel TI A system's approach to assess the exposure of agricultural production to climate change and variability SO CLIMATIC CHANGE LA English DT Article ID IPCC SRES SCENARIOS; RIVER-BASIN; WATER-RESOURCES; CROP PRODUCTION; SEASON LENGTH; VULNERABILITY; DROUGHT; IMPACTS; YIELD; TEMPERATURE AB Estimating the exposure of agriculture to climate variability and change can help us understand key vulnerabilities and improve adaptive capacity, which is vital to secure and increase world food production to feed its growing population. A number of indices to estimate exposure are available in literature. However, testing or validating them is difficult and reveals a considerable variability, and no systematic methodology has been developed to guide users in selecting indices for particular applications. This need is addressed in this paper by developing a flowchart from a conceptual model that uses a system's approach. Also, we compare five approaches to estimate exposure indices (EIs) to study the exposure of agriculture to climate variability and change: single stressor-mean climate, single stressor-extreme climate, multiple stressor-mean climate, multiple stressor-extreme climate; and combinations of the above approaches. The developed flowchart requires gathering information on the region of study, including its agriculture, stressor(s), climate factor(s) (CF), period of interest and the method of aggregation. The flowchart was applied to a case study in Kansas to better understand the five approaches to estimate EIs and the implications of the choices made in each step on the estimated the exposure. The flowchart provides options that guide EI estimation by selecting the most appropriate stressor(s), associated CF(s), and aggregation methods when a detailed methodological analysis is possible, or proposes a default method when data or resources do not allow a detailed analysis. Climate adaptation involves integration of a multitude of factors across complex systems. A more standardized approach to assessing exposure can promote information sharing across different locations and systems as this rapidly evolving area of study moves forward. C1 [Anandhi, Aavudai; Bailey, Nathaniel] Florida A&M Univ, Biol & Agr Syst Engn, Tallahassee, FL 32307 USA. [Anandhi, Aavudai; Steiner, Jean L.] ARS, USDA, Grazinglands Res Lab, 7207 West Cheyenne St, El Reno, OK 73036 USA. RP Anandhi, A (reprint author), Florida A&M Univ, Biol & Agr Syst Engn, Tallahassee, FL 32307 USA.; Anandhi, A (reprint author), ARS, USDA, Grazinglands Res Lab, 7207 West Cheyenne St, El Reno, OK 73036 USA. EM anandhi@famu.edu FU National Science Foundation [EPS-0903806]; USDA through the National Institute for Food and Agriculture's Agriculture and Food Research Initiative, Regional Approaches for Adaptation to and Mitigation of Climate Variability and Change [2013-69002-23146] FX This work was supported by the National Science Foundation under Award No. EPS-0903806 and matching support from the State of Kansas through Kansas Technology Enterprise Corporation and the Funding provided by USDA to Project No. 2013-69002-23146 through the National Institute for Food and Agriculture's Agriculture and Food Research Initiative, Regional Approaches for Adaptation to and Mitigation of Climate Variability and Change. Thanks also to the two anonymous reviewers for their thorough and insightful reviews of this paper. This is contribution number 14-308-J from the Kansas Agricultural Experiment Station. NR 62 TC 2 Z9 2 U1 6 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0165-0009 EI 1573-1480 J9 CLIMATIC CHANGE JI Clim. Change PD JUN PY 2016 VL 136 IS 3-4 BP 647 EP 659 DI 10.1007/s10584-016-1636-y PG 13 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DM5TR UT WOS:000376413600019 ER PT J AU Villasante, A Powell, MS Murdoch, GK Overturf, K Cain, K Wacyk, J Hardy, RW AF Villasante, Alejandro Powell, Madison S. Murdoch, Gordon K. Overturf, Ken Cain, Kenneth Wacyk, Jurij Hardy, Ronald W. TI Effect of anthocyanidins on myogenic differentiation in induced and non-induced primary myoblasts from rainbow trout (Oncorhynchus mykiss) SO COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY LA English DT Article DE Anthocyanidins; Rainbow trout; Skeletal muscle; Myoblasts; Myogenic differentiation ID MUSCLE STEM-CELLS; PLANT-PROTEIN-SOURCES; FISH-MEAL; SELF-RENEWAL; TOTAL REPLACEMENT; SATELLITE CELLS; NOTCH; EXPRESSION; GROWTH; RESVERATROL AB A study was conducted to test whether an anthocyanidin mixture (peonidin, cyanidin and pelargonidin chloride) modulates myogenesis in both induced and non-induced myogenic cells from juvenile rainbow trout (Oncorhynchus mykiss). We evaluated three different anthocyanidin concentrations (1 x, 2.5x and 10x) at two sampling times (24 and 36 h). To test for treatment effects, we analyzed the expression of myoD and pax7 as well as two target genes of the Notch signaling pathway, hey2 and her6. In induced myogenic cells, the lowest and middle anthocyanidin doses caused significantly greater expression of myoD after 24 h of treatment compared to control. A significantly higher expression of pax7 in cells exposed to either anthocyanidin treatment during 36 h compared was observed. Similarly, the pax7/myoD ratio was significantly lower in cells exposed to the lowest anthocyanidin doses during 24 h compared to control. No significant effect of anthocyanidin treatments on the expression of hey2 and her6 at either sampling point was detected. In non-induced cells, we observed no effect of anthocyanidins on myoD expression and significant down-regulation on pax7 expression in cells exposed to either anthocyanidin mixture concentrations after 24 and 36 h of treatment compared to control. Further, the pax7/myoD ratio was significantly lower in cells exposed to either anthocyanidin doses at both sampling time. In non-induced cells, the highest anthocyanidin dose provoked significantly greater expression of hey2 after 24 h of treatment compared to control. We detected no such effect in non-induced cells exposed to the lowest and middle anthocyanidin doses during 24 h of treatment. The expression of her6 was unaffected by anthocyanidin treatments at either sampling time or doses compared to control. Collectively, these findings provide evidence that anthocyanidins modulate specific components of the myogenic programming in fish, thereby potentially affecting somatic growth in fish fed plant-derived extracts rich in this type of polyphenols. Moreover, in early differentiating myogenic cells, the anthocyanidin effect on myogenic programming appears to differ based upon the exposure time and the differentiation stage of the myogenic cells by boosting myogenic differentiation signaling after 24 h treatment while pausing differentiation, potentially favoring cell survival after 36 h treatment. Further research to determine whether plant-derived secondary metabolites including alkaloids, terpenoids, tannins, saponins, glycosides, flavonoids, phenolics, steroids and essential oils can modulate myogenic programming in myogenic cells isolated from finfish species is warranted. (C) 2016 Elsevier Inc. All rights reserved. C1 [Villasante, Alejandro; Powell, Madison S.; Hardy, Ronald W.] Univ Idaho, Inst Aquaculture Res, Hagerman, ID 83332 USA. [Murdoch, Gordon K.] Univ Idaho, Dept Anim & Vet Sci, Moscow, ID 83844 USA. [Overturf, Ken] ARS, USDA, Hagerman Fish Culture Expt Stn, Hagerman, ID 83332 USA. [Cain, Kenneth] Univ Idaho, Inst Aquaculture Res, Moscow, ID 83844 USA. [Wacyk, Jurij] Univ Chile, Fac Ciencias Agron, Dept Anim Prod, Casilla 1004, Santiago, Chile. RP Villasante, A (reprint author), Univ Chile, Fac Ciencias Agron, Dept Anim Prod, Casilla 1004, Santiago, Chile. EM alejandro.villasante@gmail.com NR 48 TC 0 Z9 0 U1 4 U2 6 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1096-4959 EI 1879-1107 J9 COMP BIOCHEM PHYS B JI Comp. Biochem. Physiol. B-Biochem. Mol. Biol. PD JUN-JUL PY 2016 VL 196 BP 102 EP 108 DI 10.1016/j.cbpb.2016.03.004 PG 7 WC Biochemistry & Molecular Biology; Zoology SC Biochemistry & Molecular Biology; Zoology GA DM9SF UT WOS:000376705300012 PM 26969805 ER PT J AU Beck, BH Fuller, SA Li, C Green, BW Zhao, HG Rawles, SD Webster, CD Peatman, E AF Beck, Benjamin H. Fuller, S. Adam Li, Chao Green, Bartholomew W. Zhao, Honggang Rawles, Steven D. Webster, Carl D. Peatman, Eric TI Hepatic transcriptomic and metabolic responses of hybrid striped bass (Morone saxatilis x Morone chrysops) to acute and chronic hypoxic insult SO COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS LA English DT Article DE Hypoxia; Morone; Transcriptome; Bass; RNA-seq; Mitochondria ID RECEPTOR-MEDIATED ENDOCYTOSIS; DEPENDENT PROTEIN-KINASE; BHLH-PAS PROTEIN; LYSOPHOSPHATIDIC ACID; MOLECULAR-MECHANISMS; AQUATIC-HYPOXIA; GENE-EXPRESSION; IN-VITRO; APOPTOSIS; AUTOPHAGY AB Striped bass (Morone saxatilis), white bass (Morone chrysops), and their hybrid are an important group of fish prized for recreational angling in the United States, and there and abroad as a high-value farmed fish. Regardless of habitat, it is not uncommon for fish of the genus Morone to encounter and cope with conditions of scarce oxygen availability. Previously, we determined that hybrid striped bass reared under conditions of chronic hypoxia exhibited reduced feed intake, lower lipid and nutrient retention, and poor growth. To better understand the molecular mechanisms governing these phenotypes, in the present study, we examined the transcriptomic profiles of hepatic tissue in hybrid striped bass exposed to chronic hypoxia (90 days at 25% oxygen saturation) and acute hypoxia (6 h at 25% oxygen saturation). Using high-throughput RNA-seq, we found that over 1400 genes were differentially expressed under disparate oxygen conditions, with the vast majority of transcriptional changes occurring in the acute hypoxia treatment. Gene pathway and bioenergetics analyses revealed hypoxiamediated perturbation of genes and gene networks related to lipid metabolism, cell death, and changes in hepatic mitochondrial content and cellular respiration. This study offers a more comprehensive view of the temporal and tissue-specific transcriptional changes that occur during hypoxia, and reveals new and shared mechanisms of hypoxia tolerance in teleosts. Published by Elsevier Inc. C1 [Beck, Benjamin H.] ARS, USDA, Aquat Anim Hlth Res Unit, Auburn, AL 36832 USA. [Fuller, S. Adam; Green, Bartholomew W.; Rawles, Steven D.; Webster, Carl D.] ARS, USDA, Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. [Li, Chao; Zhao, Honggang; Peatman, Eric] Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, Auburn, AL 36849 USA. RP Beck, BH (reprint author), ARS, USDA, Aquat Anim Hlth Res Unit, Auburn, AL 36832 USA. EM Benjamin.Beck@ars.usda.gov NR 50 TC 0 Z9 0 U1 7 U2 9 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1744-117X EI 1878-0407 J9 COMP BIOCHEM PHYS D JI Comp. Biochem. Physiol. D-Genomics Proteomics PD JUN PY 2016 VL 18 BP 1 EP 9 DI 10.1016/j.cbd.2016.01.005 PG 9 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA DM9RG UT WOS:000376702800001 PM 26851735 ER PT J AU Fischer, JW McMurtry, D Blass, CR Walter, WD Beringer, J VerCauteren, KC AF Fischer, Justin W. McMurtry, Dan Blass, Chad R. Walter, W. David Beringer, Jeff VerCauteren, Kurt C. TI Effects of simulated removal activities on movements and space use of feral swine SO EUROPEAN JOURNAL OF WILDLIFE RESEARCH LA English DT Article DE Feral swine; Global Positioning System; Harassment; Home range; Missouri; Sus scrofa; Wild hogs ID BOAR SUS-SCROFA; WILD BOAR; PIG ERADICATION; NATIONAL-PARK; BEHAVIOR; POPULATION; HELICOPTER; MANAGEMENT; RESOURCE; HABITAT AB Abundance and distribution of feral swine (Sus scrofa) in the USA have increased dramatically during the last 30 years. Effective measures are needed to control and eradicate feral swine populations without displacing animals over wider areas. Our objective was to investigate effects of repeated simulated removal activities on feral swine movements and space use. We analyzed location data from 21 feral swine that we fitted with Global Positioning System harnesses in southern MO, USA. Various removal activities were applied over time to eight feral swine before lethal removal, including trapped-and-released, chased with dogs, chased with hunter, and chased with helicopter. We found that core space-use areas were reduced following the first removal activity, whereas overall space-use areas and diurnal movement distances increased following the second removal activity. Mean geographic centroid shifts did not differ between pre- and post-periods for either the first or second removal activities. Our information on feral swine movements and space use precipitated by human removal activities, such as hunting, trapping, and chasing with dogs, helps fill a knowledge void and will aid wildlife managers. Strategies to optimize management are needed to reduce feral swine populations while preventing enlarged home ranges and displacing individuals, which could lead to increased disease transmission risk and human-feral swine conflict in adjacent areas. C1 [Fischer, Justin W.; Blass, Chad R.; VerCauteren, Kurt C.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. [McMurtry, Dan] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, 1714 Commerce Ct Suite C, Columbia, MO 65202 USA. [Walter, W. David] Penn State Univ, US Geol Survey, Penn Cooperat Fish & Wildlife Res Unit, 403 Forest Resources Bldg, University Pk, PA 16802 USA. [Beringer, Jeff] Missouri Dept Conservat, 1110 S Coll Ave, Columbia, MO 65201 USA. RP VerCauteren, KC (reprint author), Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM kurt.c.vercautern@aphis.usda.gov FU Missouri Department of Conservation; National Wildlife Research Center; USDA/APHIS/Wildlife Services (MO) FX We thank the many coworkers and volunteers who assisted with the project design and data collection, especially C. Arias, J. Blair, and other staff from the Missouri Department of Conservation. Research was funded in part by the Missouri Department of Conservation, USDA/APHIS/Wildlife Services (MO), and the National Wildlife Research Center. NR 44 TC 0 Z9 0 U1 16 U2 25 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1612-4642 EI 1439-0574 J9 EUR J WILDLIFE RES JI Eur. J. Wildl. Res. PD JUN PY 2016 VL 62 IS 3 BP 285 EP 292 DI 10.1007/s10344-016-1000-6 PG 8 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA DM8DZ UT WOS:000376592300004 ER PT J AU Lietz, G Furr, HC Gannon, BM Green, MH Haskell, M Lopez-Teros, V Novotny, JA Palmer, AC Russell, RM Tanumihardjo, SA Van Loo-Bouwman, CA AF Lietz, Georg Furr, Harold C. Gannon, Bryan M. Green, Michael H. Haskell, Marjorie Lopez-Teros, Veronica Novotny, Janet A. Palmer, Amanda C. Russell, Robert M. Tanumihardjo, Sherry A. Van Loo-Bouwman, Carolien A. TI Current Capabilities and Limitations of Stable Isotope Techniques and Applied Mathematical Equations in Determining Whole-Body Vitamin A Status SO FOOD AND NUTRITION BULLETIN LA English DT Review DE model-based compartmental analysis; prediction equations; retinol isotope dilution; vitamin A status ID RETINOL-BINDING-PROTEIN; IONIZATION MASS-SPECTROMETRY; KINETIC-ANALYSIS SHOWS; BETA-CAROTENE; PLASMA RETINOL; DILUTION TECHNIQUE; ZINC SUPPLEMENTATION; LIVER STORES; HEPATOCELLULAR-CARCINOMA; COMPARTMENTAL ANALYSIS AB Background: Retinol isotope dilution (RID) methodology provides a quantitative estimate of total body vitamin A (VA) stores and is the best method currently available for assessing VA status in adults and children. The methodology has also been used to test the efficacy of VA interventions in a number of low-income countries. Infections, micronutrient deficiencies (eg, iron and zinc), liver disease, physiological age, pregnancy, and lactation are known or hypothesized to influence the accuracy of estimating total body VA stores using the isotope dilution technique. Objective: Our objectives were to review the strengths and limitations of RID methods, to discuss what is known about the impact of various factors on results, and to summarize contributions of model-based compartmental analysis to assessing VA status. Methods: Relevant published literature is reviewed and discussed. Results: Various equations and compartmental modeling have been used to estimate the total body VA stores using stable isotopes, including a newer 3-day equation that provides an estimate of total body VA stores in healthy adults. At present, there is insufficient information on absorption of the isotope tracer, and there is a need to further investigate how various factors impact the application of RID techniques in field studies. Conclusions: Isotope dilution methodology can provide useful estimates of total body VA stores in apparently healthy populations under controlled study conditions. However, more research is needed to determine whether the method is suitable for use in settings where there is a high prevalence of infection, iron deficiency, and/or liver disease. C1 [Lietz, Georg] Newcastle Univ, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England. [Furr, Harold C.; Gannon, Bryan M.; Tanumihardjo, Sherry A.] Univ Wisconsin, Madison, WI USA. [Green, Michael H.] Penn State Univ, Dept Nutr Sci, University Pk, PA 16802 USA. [Haskell, Marjorie] Univ Calif Davis, Program Int & Community Nutr, Davis, CA 95616 USA. [Haskell, Marjorie] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Lopez-Teros, Veronica] Univ Sonora, Hermosillo 83000, Sonora, Mexico. [Novotny, Janet A.] USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. [Palmer, Amanda C.] Johns Hopkins Bloomberg Sch Publ Hlth, Baltimore, MD USA. [Russell, Robert M.] Tufts Univ, Boston, MA 02111 USA. [Van Loo-Bouwman, Carolien A.] Radboud Univ Nijmegen, Med Ctr, NL-6525 ED Nijmegen, Netherlands. RP Lietz, G (reprint author), Newcastle Univ, Sch Agr Food & Rural Dev, Human Nutr Res Ctr, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England. EM georg.lietz@ncl.ac.uk FU International Atomic Energy Agency; Bill & Melinda Gates Foundation; Global Health funds at the University of Wisconsin-Madison FX The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Financial support for this manuscript was provided by the International Atomic Energy Agency, the Bill & Melinda Gates Foundation, and Global Health funds at the University of Wisconsin-Madison (S.A.T.). NR 124 TC 1 Z9 1 U1 0 U2 3 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0379-5721 EI 1564-8265 J9 FOOD NUTR BULL JI Food Nutr. Bull. PD JUN PY 2016 VL 37 SU 2 BP S87 EP S103 DI 10.1177/0379572116630642 PG 17 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA DM9EG UT WOS:000376667000003 PM 27053491 ER PT J AU Haddad, NJ Batainh, A Saini, D Migdadi, O Aiyaz, M Manchiganti, R Krishnamurthy, V Al-Shagour, B Brake, M Bourgeois, L De Guzman, L Rinderer, T Hamouri, ZM AF Haddad, Nizar Jamal Batainh, Ahmed Saini, Deepti Migdadi, Osama Aiyaz, Mohamed Manchiganti, Rushiraj Krishnamurthy, Venkatesh Al-Shagour, Banan Brake, Mohammad Bourgeois, Lelania De Guzman, Lilia Rinderer, Thomas Hamouri, Zayed Mahoud TI Evaluation of Apis mellifera syriaca Levant region honeybee conservation using comparative genome hybridization SO GENETICA LA English DT Article DE Apis mellifera syriaca; Array aCGH; SNP; Varroa resistance; Dry conditions ID VARROA-DESTRUCTOR; PROVIDES INSIGHT; BEE COLONIES; DEFENSES; MITE AB Apis mellifera syriaca is the native honeybee subspecies of Jordan and much of the Levant region. It expresses behavioral adaptations to a regional climate with very high temperatures, nectar dearth in summer, attacks of the Oriental wasp and is resistant to Varroa mites. The A. m. syriaca control reference sample (CRS) in this study was originally collected and stored since 2001 from "Wadi Ben Hammad", a remote valley in the southern region of Jordan. Morphometric and mitochondrial DNA markers of these honeybees had shown highest similarity to reference A. m. syriaca samples collected in 1952 by Brother Adam of samples collected from the Middle East. Samples 1-5 were collected from the National Center for Agricultural Research and Extension breeding apiary which was established for the conservation of A. m. syriaca. Our objective was to determine the success of an A. m. syriaca honey bee conservation program using genomic information from an array-based comparative genomic hybridization platform to evaluate genetic similarities to a historic reference collection (CRS). Our results had shown insignificant genomic differences between the current population in the conservation program and the CRS indicated that program is successfully conserving A. m. syriaca. Functional genomic variations were identified which are useful for conservation monitoring and may be useful for breeding programs designed to improve locally adapted strains of A. m. syriaca. C1 [Haddad, Nizar Jamal; Batainh, Ahmed; Al-Shagour, Banan] Natl Ctr Agr Res & Extens, Bee Res Dept, POB 639, Baqa 19381, Jordan. [Saini, Deepti; Aiyaz, Mohamed; Manchiganti, Rushiraj; Krishnamurthy, Venkatesh] Genotyp Technol P Ltd, Res & Dev Unit, 259 Apurva,2nd Floor,4th Cross,RMV 2nd Stage, Bangalore 560094, Karnataka, India. [Migdadi, Osama; Hamouri, Zayed Mahoud] Natl Ctr Agr Res & Extens, Jordanian Bee Res Stn, Irbid, Jordan. [Brake, Mohammad] Jerash Univ, Fac Sci, Dept Sci, Jerash, Jordan. [Bourgeois, Lelania; De Guzman, Lilia; Rinderer, Thomas] USDA ARS, Honey Bee Breeding Genet & Physiol Lab, 1157 Ben Hur Rd, Baton Rouge, LA 70820 USA. RP Haddad, NJ (reprint author), Natl Ctr Agr Res & Extens, Bee Res Dept, POB 639, Baqa 19381, Jordan. EM drnizarh@gmail.com RI Niranjan, Vidya/O-6464-2016 OI Niranjan, Vidya/0000-0001-9187-7753 FU USAID [TA-MOU-09-M29-075, TA-MOU-11-M32-035] FX This research paper is part of the project entitled "Evaluation and Development of Apis mellifera syriaca with Useful keeping Traits for the Middle East Region." And the project entitled "Impact of the Varroa destructor infestation and its viral load on honeybee colonies in the Middle East and North Africa" funded by USAID grants numbers: TA-MOU-09-M29-075 and TA-MOU-11-M32-035. NR 24 TC 0 Z9 0 U1 5 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0016-6707 EI 1573-6857 J9 GENETICA JI Genetica PD JUN PY 2016 VL 144 IS 3 BP 279 EP 287 DI 10.1007/s10709-016-9897-y PG 9 WC Genetics & Heredity SC Genetics & Heredity GA DM9LQ UT WOS:000376688200003 PM 27010806 ER PT J AU Egamberdiev, SS Saha, S Salakhutdinov, I Jenkins, JN Deng, D Abdurakhmonov, IY AF Egamberdiev, Sharof S. Saha, Sukumar Salakhutdinov, Ilkhom Jenkins, Johnie N. Deng, Dewayne Abdurakhmonov, Ibrokhim Y. TI Comparative assessment of genetic diversity in cytoplasmic and nuclear genome of upland cotton SO GENETICA LA English DT Article DE Cytoplasmic genome; Chloroplast specific SSR markers; Nuclear SSR markers; Genetic diversity; Upland cotton ID GOSSYPIUM-HIRSUTUM L.; SIMPLE SEQUENCE REPEAT; FIBER QUALITY TRAITS; CHLOROPLAST MICROSATELLITES; BIPARENTAL INHERITANCE; LINKAGE DISEQUILIBRIUM; ARABIDOPSIS-THALIANA; BARBADENSE L.; DNA VARIATION; CULTIVARS AB The importance of the cytoplasmic genome for many economically important traits is well documented in several crop species, including cotton. There is no report on application of cotton chloroplast specific SSR markers as a diagnostic tool to study genetic diversity among improved Upland cotton lines. The complete plastome sequence information in GenBank provided us an opportunity to report on 17 chloroplast specific SSR markers using a cost-effective data mining strategy. Here we report the comparative analysis of genetic diversity among a set of 42 improved Upland cotton lines using SSR markers specific to chloroplast and nuclear genome, respectively. Our results revealed that low to moderate level of genetic diversity existed in both nuclear and cytoplasm genome among this set of cotton lines. However, the specific estimation suggested that genetic diversity is lower in cytoplasmic genome compared to the nuclear genome among this set of Upland cotton lines. In summary, this research is important from several perspectives. We detected a set of cytoplasm genome specific SSR primer pairs by using a cost-effective data mining strategy. We reported for the first time the genetic diversity in the cytoplasmic genome within a set of improved Upland cotton accessions. Results revealed that the genetic diversity in cytoplasmic genome is narrow, compared to the nuclear genome within this set of Upland cotton accessions. Our results suggested that most of these polymorphic chloroplast SSRs would be a valuable complementary tool in addition to the nuclear SSR in the study of evolution, gene flow and genetic diversity in Upland cotton. C1 [Egamberdiev, Sharof S.; Salakhutdinov, Ilkhom; Abdurakhmonov, Ibrokhim Y.] Acad Sci Uzbek, Ctr Genom & Bioinformat, Tashkent 111215, Uzbekistan. [Saha, Sukumar; Jenkins, Johnie N.; Deng, Dewayne] USDA ARS, Crop Sci Res Lab, Genet & Sustainable Agr Res Unit, Mississippi State, MS 39762 USA. RP Saha, S (reprint author), USDA ARS, Crop Sci Res Lab, Genet & Sustainable Agr Res Unit, Mississippi State, MS 39762 USA. EM Sukumar.saha@ars.usda.gov RI Abdurakhmonov, Ibrokhim/F-5588-2015 OI Abdurakhmonov, Ibrokhim/0000-0001-9563-0686 FU Office of International Research Programs, U. S. Department of Agriculture (USDA) [UZB2-31016-TA-09]; U.S. Civilian Research & Development Foundation (CRDF); Cotton Incorporated, USA; Academy of Sciences of Uzbekistan within USDA-Uzbekistan FX The authors thank the Office of International Research Programs, U. S. Department of Agriculture (USDA) for providing funds for this study under research grant UZB2-31016-TA-09 and U.S. Civilian Research & Development Foundation (CRDF) and Cotton Incorporated, USA. We thank the Academy of Sciences of Uzbekistan for supporting this joint study within USDA-Uzbekistan cooperative programs. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. The U. S. Department of Agriculture is equal opportunity provider and employer. We acknowledge joint publication of USDA/ARS, and Mississippi Agricultural and Forestry Experiment Station, approved for publication as Journal Article of the Mississippi Agricultural and Forestry Experiment Station. We thank all of the public and private cotton breeders who provided seeds for this study. We would specifically like to acknowledge the help of Dr. Jack C. McCarty, Dr. Wayne Smith, Dr. Bill Meredith, Dr. Gerald O. Myers, Dr. Ted Wallace, Dr. Fred Bourland, Dr. Jack Jones, and Dr. Dick L. Auld for their help in this study by providing seeds of their released germplasm. Without their help and support we could not accomplished the goals of this research project. We also thank Dr. B. Todd Campbell and Dr. Mauricio Ulloa for their help in reviewing the manuscript. NR 70 TC 0 Z9 0 U1 3 U2 6 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0016-6707 EI 1573-6857 J9 GENETICA JI Genetica PD JUN PY 2016 VL 144 IS 3 BP 289 EP 306 DI 10.1007/s10709-016-9898-x PG 18 WC Genetics & Heredity SC Genetics & Heredity GA DM9LQ UT WOS:000376688200004 PM 27155886 ER PT J AU Rosenberg, I Schimmel, E AF Rosenberg, Irwin Schimmel, Elihu TI Bridging Basic Science and Liver Disease: Symposium SO HEPATOLOGY LA English DT Letter C1 [Rosenberg, Irwin] Tufts Univ, Sch Med, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Schimmel, Elihu] Boston Univ, Sch Med, Brookline, MA USA. RP Rosenberg, I (reprint author), Tufts Univ, Sch Med, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 1 TC 1 Z9 1 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0270-9139 EI 1527-3350 J9 HEPATOLOGY JI Hepatology PD JUN PY 2016 VL 63 IS 6 BP 2062 EP 2063 DI 10.1002/hep.27975 PG 2 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA DM5BN UT WOS:000376361800041 PM 26172988 ER PT J AU Li, JH Hunt, JF Gong, SQ Cai, ZY AF Li, Jinghao Hunt, John F. Gong, Shaoqin Cai, Zhiyong TI Fatigue behavior of wood-fiber-based tri-axial engineered sandwich composite panels (ESCP) SO HOLZFORSCHUNG LA English DT Article DE bending stiffness; fatigue behavior; residual properties; sandwich panel; S-N curve; static testing; wood-fiber-composite ID STRENGTH; DESIGN; SHEAR; BEAMS AB The static and fatigue bending behavior of wood-fiber-based tri-axial engineered sandwich composite panels (ESCP) has been investigated by four-point bending tests. Fatigue panels and weakened panels (wESCP) with an initial interface defect were manufactured for the fatigue tests. Stress sigma vs. number of cycles curves (S-N) were recorded under the different stress levels. The primary failure mode in the fatigue tests was observed in the shear zone (epoxy debonding), which was different from face failure in the pure bending zone for the static -bending test. For residual bending (RB) test, epoxy debonding failure occurred between the pure bending zone and shear zone. Macro cracks along the core/face interface developed as the number of cycles increased during the fatigue life. The crack propagation or damage for the panels submitted to fatigue test can be described as a three-stage damage process of first non-linear portion, followed by linear damage accumulation, and lastly non-linear accelerated damage. Bending stiffness degradation at the higher load level had faster degradation during fatigue life. The dissipated energy of the panels was small due to the high stiffness of the materials. C1 [Li, Jinghao; Hunt, John F.; Cai, Zhiyong] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Li, Jinghao; Gong, Shaoqin] Univ Wisconsin, Dept Biomed Engn, Wisconsin Inst Discovery, Madison, WI 53715 USA. [Li, Jinghao; Gong, Shaoqin] Univ Wisconsin, Dept Mat Sci & Engn, Madison, WI 53715 USA. RP Hunt, JF; Cai, ZY (reprint author), US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. EM jfhunt@fs.fed.us; zcai@fs.fed.us FU USDA, Forest Products Laboratory FX This work is supported by USDA, Forest Products Laboratory and the authors gratefully acknowledge the support of Sara Fishwild, James Bridwell, Marshall Begel, Dave Simpson, and Marc Joyal of EMRSL group for the mechanical testing. NR 30 TC 3 Z9 3 U1 3 U2 7 PU WALTER DE GRUYTER GMBH PI BERLIN PA GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY SN 0018-3830 EI 1437-434X J9 HOLZFORSCHUNG JI Holzforschung PD JUN PY 2016 VL 70 IS 6 BP 567 EP 575 DI 10.1515/hf-2015-0091 PG 9 WC Forestry; Materials Science, Paper & Wood SC Forestry; Materials Science GA DM9IY UT WOS:000376680300010 ER PT J AU Fanatico, AC Owens-Hanning, CM Gunsaulis, VB Donoghue, AM AF Fanatico, A. C. Owens-Hanning, C. M. Gunsaulis, V. Brewer Donoghue, A. M. TI Choice feeding of protein concentrate and grain to organic meat chickens SO JOURNAL OF APPLIED POULTRY RESEARCH LA English DT Article DE free-range; organic; poultry; feeding; nutrient self-selection; ecology ID DIETS; PERFORMANCE; ENERGY AB In alternative poultry production, such as free-range and organic, alternative feedingmethods may be useful. Instead of a single fully Formulated diet, a "Choice" method offers 2 complementary feeds, a protein concentrate and a grain, between which birds self-select. This method was used in the past and may allow free-range birds that encounter diverse environmental conditions to adjust their diets to meet changing nutritional needs. A trial was conducted to study the impact of Choice feeding organic meat chickens vs. Formulated feeding. Chicks were raised to 64 d in an organic research facility with outdoor access. Birds in the Choice treatment were offered cracked corn and, separately, a concentrate composed of roasted soybeans, fishmeal, and mineral and vitamin premix. During the trial, Choice birds self-selected a diet that was low in protein (only 12% CP during the last week of production). Live weight gains, as well as carcass and breast weights, were lower for birds in the Choice treatment compared to the Formulated treatment; however, FCR was superior for Choice and yields did not differ. The cost of Choice feed was lower than Formulated ($0.58/kg vs. $0.66/kg, respectively). Results indicate that Choice feeding may be useful in alternative and free-range poultry production, especially in organic production. It enables the use of farm-raised feeds, important for on-farm nutrient cycling and reducing the cost of expensive organic feed. C1 [Fanatico, A. C.] Appalachian State Univ, Sustainable Dev Dept, Boone, NC 28608 USA. [Owens-Hanning, C. M.; Gunsaulis, V. Brewer] Univ Arkansas, Dept Poultry Sci, Fayetteville, AR 72701 USA. [Donoghue, A. M.] ARS, Poultry Prod & Product Safety Res Unit, USDA, Fayetteville, AR USA. RP Fanatico, AC (reprint author), Appalachian State Univ, Sustainable Dev Dept, Boone, NC 28608 USA. EM fanaticoac@appstate.edu FU U.S. Department of Agriculture Sustainable Agriculture and Research (Southern Region) [LS10-226]; Organic Research and Extension Initiative Program [2011-01955] FX Funded by a grant from the U.S. Department of Agriculture Sustainable Agriculture and Research (Southern Region) LS10-226 and Organic Research and Extension Initiative Program 2011-01955. NR 30 TC 1 Z9 1 U1 5 U2 11 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1056-6171 EI 1537-0437 J9 J APPL POULTRY RES JI J. Appl. Poult. Res. PD JUN PY 2016 VL 25 IS 2 BP 156 EP 164 DI 10.3382/japr/pfv076 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA DM6BO UT WOS:000376435100003 ER PT J AU Zhai, W Peebles, ED Wang, X Gerard, PD Olanrewaju, HA Mercier, Y AF Zhai, W. Peebles, E. D. Wang, X. Gerard, P. D. Olanrewaju, H. A. Mercier, Y. TI Effects of dietary lysine and methionine supplementation on Ross 708 male broilers from 21 to 42 d of age (III): serum metabolites, hormones, and their relationship with growth performance SO JOURNAL OF APPLIED POULTRY RESEARCH LA English DT Article DE broiler; growth; IGF1; methionine; testosterone ID DENSITY-LIPOPROTEIN CHOLESTEROL; FACTOR-I; NITROGEN-EXCRETION; MUSCLE DEVELOPMENT; LIPID-METABOLISM; FACTORS IGF; URIC-ACID; CHICKENS; INSULIN; PROTEIN AB A previous study has shown that a limited increase of lysine (Lys) and methionine (Met) in broiler diets may improve feed conversion ratio (FCR), BW, carcass yield, and breastmeat yield. This study was conducted to determine the effects of dietary Lys and Met supplementation on various blood serum metabolites and hormones, and their relationship with growth performance and the meat yield of male Ross x Ross 708 broilers. Experimental diets, including 2 Lys levels (100 and 120% of recommended levels) and 4 Met levels (80, 100, 120, and 140% of recommended levels), were fed from 21 to 42 d of age. Blood serum metabolites were not affected by any of the dietary treatments. Uric acid concentration was negatively correlated with absolute weights of the whole body, carcass, pectoralis minor, wing, leg quarter, and front half parts. Cholesterol was negatively correlated with pectoralis major, wing, pectoralis major+minor, and front half. Insulin-like growth factor I (IGF1) and testosterone concentrations were found to be positively related to BW. In addition, positive correlations occurred between concentrations of total protein and high-density lipoprotein cholesterol; uric acid and total cholesterol; and IGF1 and testosterone. In conclusion, dietary amino acid supplementation did not affect the blood serum metabolites investigated, which may be due to the fact that these metabolites are highly regulated in the blood. However, because the expression of genes for these metabolites may be unique to individual birds, growth and the deposition of specific muscles among birds can be quite variable. Even though Lys and Met supplementation improved growth performance, and BW was positively related to circulating IGF1 and testosterone concentrations, these 2 hormones were not affected by dietary Met supplementation. The increase in growth rate caused by dietary Lys or Met supplementation may be due to changes in various integral metabolic pathways as a result of polygenic and pleiotropic relationships, rather than by the isolated effects of individual hormones and metabolites. C1 [Zhai, W.; Peebles, E. D.; Wang, X.] Mississippi State Univ, Dept Poultry Sci, Mississippi State, MS 39762 USA. [Gerard, P. D.] Clemson Univ, Dept Math Sci, Clemson, SC 29634 USA. [Olanrewaju, H. A.] ARS, USDA, Poultry Res Unit, Mississippi State, MS 39762 USA. [Mercier, Y.] Adisseo France SAS CERN, 6 Route Noire, F-03600 Malicorne, France. RP Zhai, W (reprint author), Mississippi State Univ, Dept Poultry Sci, Mississippi State, MS 39762 USA. EM wei.zhai@msstate.edu FU Adisseo France FX This is journal article number J-12547 from the Mississippi Agricultural and Forestry Experiment Station (Mississippi State), under MIS-322280 and financially supported by Adisseo France. Use of trade names in this publication does not imply endorsement by the Mississippi Agricultural and Forestry Experiment Station of the products, nor similar ones not mentioned. The authors wish to express their gratitude to Ms. Sharon Womack and Ms. Donna Morgan and the Poultry Research Center farm crew (Mississippi State University) for their technical assistance during this research. NR 36 TC 1 Z9 1 U1 5 U2 8 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1056-6171 EI 1537-0437 J9 J APPL POULTRY RES JI J. Appl. Poult. Res. PD JUN PY 2016 VL 25 IS 2 BP 223 EP 231 DI 10.3382/japr/pfw004 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA DM6BO UT WOS:000376435100010 ER PT J AU de Oliveira, JG Sarria, ALF Ferreira, LL Caulfield, JC Powers, SJ Pickett, JA de Leon, AAP Birkett, MA Borges, LMF AF de Oliveira Filho, Jaires Gomes Franceschini Sarria, Andre Lucio Ferreira, Lorena Lopes Caulfield, John C. Powers, Stephen J. Pickett, John A. Perez de Leon, Adalberto A. Birkett, Michael A. Ferreira Borges, Ligia Miranda TI Quantification of brown dog tick repellents, 2-hexanone and benzaldehyde, and release from tick-resistant beagles, Canis lupus familiaris SO JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES LA English DT Article DE 2-Hexanone; Benzaldehyde; VOC's; Rhipicephalus sanguineus sensu lato; Non-host; GC-MS ID RHIPICEPHALUS-SANGUINEUS ACARI; NONPREFERRED WATERBUCK HOSTS; PREFERRED BUFFALO; IXODIDAE; IDENTIFICATION; APOSEMATISM; PREVENTION; ECOLOGY; SIGNALS; BRAZIL AB We have recently shown that repellency of the tick Rhipicephalus sanguineus sensu lato by the tick resistant dog breed, the beagle, is mediated by volatile organic compounds (VOCs) 2-hexanone and benzaldehyde present in beagle odour. Ectoparasite location of animal hosts is affected by variation in these odour components and their ratios. The aim of this study was to quantify the release rate, and the ratio, of 2-hexanone and benzaldehyde from beagles. The odour of three beagles was collected, for four days, over one week (day 0, day 1, day 4 and day 7). The compounds were identified using coupled high-resolution gas chromatography-mass spectrometry (GC-MS), and authentic standards of compounds were used to generate external calibration curves for quantification. Both compounds were found in all dogs on all days. The amount of benzaldehyde was always higher than that of 2-hexanone and so their ratio varied from unity, on average (over time) being 3.128 +/- 0.365, 1.902 +/- 0.390, 1.670 +/- 0.671 ng mL(-1) for beagle 1, 2 and 3, respectively. There was no significant (p < 0.05, F-test) effect of time. The overall mean was 2.233 +/- 0.387 ng mL(-1). These results further previous findings by documenting the presence of 2-hexanone and benzaldehyde in beagle odour samples covering a 7-day period. This knowledge enables development of repellents to protect dogs from R. sanguineus s. l. infestation. (C) 2016 Elsevier B.V. All rights reserved. C1 [de Oliveira Filho, Jaires Gomes; Ferreira, Lorena Lopes] Univ Fed Goias, Escola Vet & Zootecnia, Campus 2,Cx Postal 131, BR-74001970 Goiania, Go, Brazil. [Franceschini Sarria, Andre Lucio; Caulfield, John C.; Pickett, John A.; Birkett, Michael A.] Rothamsted Res, Biol Chem & Crop Protect Dept, Harpenden AL5 2JQ, Herts, England. [Powers, Stephen J.] Rothamsted Res, Computat & Syst Biol Dept, Harpenden AL5 2JQ, Herts, England. [Perez de Leon, Adalberto A.] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, Vet Pest Genom Ctr, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. [Ferreira Borges, Ligia Miranda] Univ Fed Goias, Inst Patol Trop & Saude Publ, Setor Univ, Rua 235 S-N, BR-74605050 Goiania, Go, Brazil. RP Borges, LMF (reprint author), Univ Fed Goias, Inst Patol Trop & Saude Publ, Setor Univ, Rua 235 S-N, BR-74605050 Goiania, Go, Brazil. EM jaires_filho@hotmail.com; andre.sarria@rothamsted.ac.uk; loren4_lopes@hotmail.com; john.caulfield@rothamsted.ac.uk; stephen.powers@rothamsted.ac.uk; john.pickett@rothamsted.ac.uk; beto.perezdeleon@ars.usda.gov; mike.birkett@rothamsted.ac.uk; borges.ligia@gmail.com RI Franceschini Sarria, Andre Lucio/C-8189-2012 OI Franceschini Sarria, Andre Lucio/0000-0003-0616-624X FU National Council of Technological and Scientific Development (CNPq), Brazil [2013-9, 2015-0]; Biotechnology and Biological Sciences Research Council (BBSRC) of the United Kingdom FX We thank the National Council of Technological and Scientific Development (CNPq) (Grant no. #/2013-9), Brazil, for its financial support of this project and for supply a Scholarship to LMFB (#/2015-0). Rothamsted Research receives grant-aided support from the Biotechnology and Biological Sciences Research Council (BBSRC) of the United Kingdom. USDA is an equal opportunity provider and employer. NR 43 TC 0 Z9 0 U1 3 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1570-0232 EI 1873-376X J9 J CHROMATOGR B JI J. Chromatogr. B PD JUN 1 PY 2016 VL 1022 BP 64 EP 69 DI 10.1016/j.jchromb.2016.03.014 PG 6 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA DM7MX UT WOS:000376545800009 PM 27085013 ER PT J AU Rudnick, D Irmak, S Ferguson, R Shaver, T Djaman, K Slater, G Bereuter, A Ward, N Francis, D Schmer, M Wienhold, B Van Donk, S AF Rudnick, Daran Irmak, Suat Ferguson, Richard Shaver, Tim Djaman, Koffi Slater, Glen Bereuter, Aaron Ward, Nicholas Francis, Dennis Schmer, Marty Wienhold, Brian Van Donk, Simon TI Economic Return versus Crop Water Productivity of Maize for Various Nitrogen Rates under Full Irrigation, Limited Irrigation, and Rainfed Settings in South Central Nebraska SO JOURNAL OF IRRIGATION AND DRAINAGE ENGINEERING LA English DT Article DE Crop water productivity; Crop water use efficiency; Economic analysis; Evapotranspiration; Irrigation water use efficiency; Limited irrigation; Maize; Nitrogen ID CORN YIELD RESPONSE; USE EFFICIENCY; DEFICIT IRRIGATION; MANAGEMENT STRATEGIES; NITRATE-NITROGEN; SOIL; EVAPOTRANSPIRATION; FERTILIZATION; RECOMMENDATIONS; ENVIRONMENT AB Field research was conducted at the University of Nebraska-Lincoln South Central Agricultural Laboratory (SCAL) located near Clay Center, NE, in the growing seasons of 2011 to 2014. A partial economic analysis was conducted for maize (Zea mays L.) at nitrogen (N) fertilizer treatments of 0, 84, 140, 196, and 252 kg ha(-1) under full irrigation (FIT), limited irrigation (75% FIT), and rainfed settings for all growing seasons and then compared to crop water productivity (CWP) measured as crop water use efficiency (CWUE) and irrigation water use efficiency (IWUE). Nitrogen fertilizer increased CWUE and IWUE in all growing seasons. The CWUE values ranged from 0.90 to 2.81 kg m(-3) and the IWUE values ranged from -1.01 to 3.24 kg m(-3). Operational costs and net income varied among treatments and across years. Irrigation and N fertilizer rate had an interacting effect (P-0.05 < 0.05) on both gross and net income in 2011, 2012, and 2013. Net income was maximized under rainfed settings with a N fertilizer rate between 140 (dry year) to 196 kg ha(-1)(wet year); in contrast, the optimal N fertilizer rate under irrigated conditions was 196 kg ha(-1). Positive relationships were observed between relative net income (RNI) and CWUE with R-2 values of 0.72, 0.87, and 0.84 for rainfed, 75% FIT, and FIT, respectively, as well as between RNI and IWUE with an R-2 value of 0.96 for 75% FIT and 0.86 for FIT. Lower CWUE and IWUE values were associated with lower RNI. With consideration to CWUE, IWUE, and partial factor productivity of N, full irrigation management is recommended under non-water-limiting conditions and limited/deficit irrigation management strategies (e.g., 75% FIT) under water-limiting conditions, with N fertilizer rate not exceeding 196 kg ha(-1) to achieve a high economic return for the study area. (C) 2016 American Society of Civil Engineers. C1 [Rudnick, Daran; Irmak, Suat] Univ Nebraska, Dept Biol Syst Engn, 239 LW Chase Hall, Lincoln, NE 68583 USA. [Ferguson, Richard; Slater, Glen; Ward, Nicholas] Univ Nebraska, Dept Agron & Hort, Lincoln, NE USA. [Shaver, Tim] Univ Nebraska Lincoln, West Cent Res & Extens Ctr, North Platte, NE USA. [Djaman, Koffi] Africa Rice Ctr AfricaRice, St Louis, Senegal. [Bereuter, Aaron] Monsanto Co, Norfolk, NE USA. [Francis, Dennis; Schmer, Marty; Wienhold, Brian] Univ Nebraska, USDA ARS, Agroecosyst Management Unit, Lincoln, NE 68583 USA. [Van Donk, Simon] Iteris, Grand Forks, ND USA. RP Irmak, S (reprint author), Univ Nebraska, Dept Biol Syst Engn, 239 LW Chase Hall, Lincoln, NE 68583 USA. EM sirmak2@unl.edu NR 59 TC 0 Z9 0 U1 13 U2 18 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9437 EI 1943-4774 J9 J IRRIG DRAIN ENG JI J. Irrig. Drainage Eng-ASCE PD JUN PY 2016 VL 142 IS 6 AR 04016017 DI 10.1061/(ASCE)IR.1943-4774.0001023 PG 12 WC Agricultural Engineering; Engineering, Civil; Water Resources SC Agriculture; Engineering; Water Resources GA DM6DG UT WOS:000376439900006 ER PT J AU Duarte, A Seliwiorstow, T Miller, WG De Zutter, L Uyttendaele, M Dierick, K Botteldoorn, N AF Duarte, Alexandra Seliwiorstow, Tomasz Miller, William G. De Zutter, Lieven Uyttendaele, Mieke Dierick, Katelijne Botteldoorn, Nadine TI Discriminative power of Campylobacter phenotypic and genotypic typing methods SO JOURNAL OF MICROBIOLOGICAL METHODS LA English DT Article DE Broiler meat; Campylobacter coli; Campylobacter jejuni; Typing methods; Food; MLST; flaA-RFLP; Antimicrobial resistance ID FIELD GEL-ELECTROPHORESIS; MULTILOCUS SEQUENCE TYPES; CLONAL COMPLEXES; CHICKEN MEAT; ANTIBIOTIC SUSCEPTIBILITY; ANTIMICROBIAL RESISTANCE; MOLECULAR EPIDEMIOLOGY; GENETIC DIVERSITY; UNITED-KINGDOM; POULTRY ORIGIN AB The aim of this study was to compare different typing methods, individually and combined, for use in the monitoring of Campylobacter in food. Campylobacter jejuni (n = 94) and Campylobacter coli (n = 52) isolated from different broiler meat carcasses were characterized using multilocus sequence typing (MIST), flagellin gene A restriction fragment length polymorphism typing (flaA-RFLP), antimicrobial resistance profiling (AMRp), the presence/absence of 5 putative virulence genes; and, exclusively for C. jejuni, the determination of lipooligosaccharide (LOS) class. Discriminatory power was calculated by the Simpson's index of diversity (SID) and the congruence was measured by the adjusted Rand index and adjusted Wallace coefficient. MLST was individually the most discriminative typing method for both C. jejuni (SID = 0.981) and C coli (SID = 0.957). The most discriminative combination with a SID of 0.992 for both C. jejuni and C. coli was obtained by combining MLST withflaA-RFLP. The combination of MIST with flaA-RFLP is an easy and feasible typing method for short-term monitoring of Campylobacter in broiler meat carcass. (C) 2016 Elsevier B.V. All rights reserved. C1 [Duarte, Alexandra; Seliwiorstow, Tomasz; Uyttendaele, Mieke] Univ Ghent, Fac Biosci Engn, Dept Food Safety & Food Qual, Lab Food Microbiol & Food Preservat, Coupure Links 653, B-9000 Ghent, Belgium. [Duarte, Alexandra; Dierick, Katelijne; Botteldoorn, Nadine] Inst Publ Hlth, Sci Serv Food Borne Pathogens, Juliette Wytsman St 14, B-1050 Brussels, Belgium. [Seliwiorstow, Tomasz; De Zutter, Lieven] Univ Ghent, Fac Vet Med, Dept Vet Publ Hlth & Food Safety, Salisburylaan 133, B-9820 Merelbeke, Belgium. [Miller, William G.] ARS, USDA, WRRC, Produce Safety & Microbiol, 800 Buchanan St, Albany, CA 94710 USA. RP Duarte, A (reprint author), Univ Ghent, Fac Biosci Engn, Dept Food Safety & Food Qual, Lab Food Microbiol & Food Preservat, Coupure Links 653, B-9000 Ghent, Belgium.; Duarte, A (reprint author), Inst Publ Hlth, Sci Serv Food Borne Pathogens, Juliette Wytsman St 14, B-1050 Brussels, Belgium. FU Belgian Federal Public Service of Health, Food Chain Safety and Environment [CAMPYTRACE RF 11/6241] FX This work was funded by the Belgian Federal Public Service of Health, Food Chain Safety and Environment under the project CAMPYTRACE RF 11/6241. We would like also to thank to Amory Wuyts, Emma Yee and Elke Wattijn for their excellent technical assistance. NR 56 TC 0 Z9 0 U1 9 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-7012 EI 1872-8359 J9 J MICROBIOL METH JI J. Microbiol. Methods PD JUN PY 2016 VL 125 BP 33 EP 39 DI 10.1016/j.mimet.2016.03.004 PG 7 WC Biochemical Research Methods; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA DM9RA UT WOS:000376702200006 PM 26996762 ER PT J AU Windels, SK Pittman, HT Grubb, TG Grim, LH Bowerman, WW AF Windels, Steve K. Pittman, H. Tyler Grubb, Teryl G. Grim, Leland H. Bowerman, William W. TI BALD EAGLE PREDATION ON DOUBLE-CRESTED CORMORANT AND HERRING GULL EGGS SO JOURNAL OF RAPTOR RESEARCH LA English DT Letter DE Bald Eagle; Haliaeetus leucocephalus; Herring Gull; Larus argentatus; Double-crested Cormorant; Phalacrocorax auritus; egg predation; Minnesota C1 [Windels, Steve K.; Grim, Leland H.] Voyageurs Natl Pk, 360 Highway 11 E, Int Falls, MN 56649 USA. [Pittman, H. Tyler; Bowerman, William W.] Univ Maryland, Dept Environm Sci & Technol, 1426 Anim Sci Bldg, College Pk, MD 20742 USA. [Grubb, Teryl G.] US Forest Serv, USDA, Rocky Mt Res Stn, 2500 South Pine Knoll Dr, Flagstaff, AZ 86001 USA. RP Windels, SK (reprint author), Voyageurs Natl Pk, 360 Highway 11 E, Int Falls, MN 56649 USA. EM steve_windels@nps.gov NR 11 TC 0 Z9 0 U1 9 U2 10 PU RAPTOR RESEARCH FOUNDATION INC PI HASTINGS PA 14377 117TH STREET SOUTH, HASTINGS, MN 55033 USA SN 0892-1016 EI 2162-4569 J9 J RAPTOR RES JI J. Raptor Res. PD JUN PY 2016 VL 50 IS 2 BP 230 EP 232 PG 3 WC Ornithology SC Zoology GA DN0MV UT WOS:000376758900014 ER PT J AU Biswas, A Alves, CR Trevisan, MTS Berfield, J Furtado, RF Liu, ZS Cheng, HN AF Biswas, Atanu Alves, Carlucio R. Trevisan, Maria T. S. Berfield, Janet Furtado, Roselayne F. Liu, Zengshe Cheng, Huai N. TI Derivatives of Cardanol through the Ene Reaction with Diethyl Azodicarboxylate SO JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY LA English DT Article DE cardanol; cashew nut shell liquid; diethyl azodicarboxylate; ene reaction ID NUT SHELL LIQUID; MODIFIED SOYBEAN OIL; EPOXY-RESIN; RENEWABLE RESOURCE; ORGANIC-SYNTHESIS; POLYURETHANES; POLYMERS; FORMALDEHYDE; SIMULATION; ESTER AB Cardanol is an alkyl/alkenyl phenolic material obtained from cashew nut shell liquid (CNSL), which is a byproduct of cashew nut processing. In an effort to develop new uses, cardanol was derivatized for the first time with diethyl azodicarboxylate (DEAD) through the ene reaction. The reaction was facile and required only the application of heat without a catalyst. Both conventional heating and microwave heating were shown to be effective; the latter entailed much shorter reaction time and substantial energy savings. The reaction product (a hydrazino-ester derivative of cardanol) was characterized by nuclear magnetic resonance (NMR). The product increased in viscosity with time and may be useful as a viscosifier in oil-based commercial formulations and as a synthon for further organic reactions. C1 [Biswas, Atanu; Berfield, Janet; Liu, Zengshe] USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. [Alves, Carlucio R.] Univ Estadual Ceara, Dept Quim, Ave Silas Munguba 1700,Campus Itaperi, BR-60740020 Fortaleza, Ceara, Brazil. [Trevisan, Maria T. S.] Univ Fed Ceara, Dept Quim, Campus Pici,CP 6021, BR-60455760 Fortaleza, Ceara, Brazil. [Furtado, Roselayne F.] Embrapa Agroind Trop, Rua Dra Sara Mesquita 2270, BR-60511110 Fortaleza, Ceara, Brazil. [Cheng, Huai N.] USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. RP Biswas, A (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA.; Cheng, HN (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM atanu.biswas@ars.usda.gov; hn.cheng@ars.usda.gov FU CNPq [405506/2013-9] FX The authors thank A. J. Thomas for viscosity measurements, Karl Vermillion for NMR data, and Prof Omar El Seoud (University of Sao Paulo) for helpful suggestions. Atanu Biswas and Roselayne Ferro Furtado thank CNPq (process No. 405506/2013-9) for the support of this work through the Science without Borders program. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 30 TC 0 Z9 0 U1 4 U2 9 PU SOC BRASILEIRA QUIMICA PI SAO PAULO PA CAIXA POSTAL 26037, 05599-970 SAO PAULO, BRAZIL SN 0103-5053 EI 1678-4790 J9 J BRAZIL CHEM SOC JI J. Braz. Chem. Soc. PD JUN PY 2016 VL 27 IS 6 BP 1078 EP 1082 DI 10.5935/0103-5053.20160003 PG 5 WC Chemistry, Multidisciplinary SC Chemistry GA DM8GV UT WOS:000376600600013 ER PT J AU Araujo, WL Creason, AL Mano, ET Camargo-Neves, AA Minami, SN Chang, JH Loper, JE AF Araujo, Welington L. Creason, Allison L. Mano, Emy T. Camargo-Neves, Aline A. Minami, Sonia N. Chang, Jeff H. Loper, Joyce E. TI Genome Sequencing and Transposon Mutagenesis of Burkholderia seminalis TC3.4.2R3 Identify Genes Contributing to Suppression of Orchid Necrosis Caused by B-gladioli SO MOLECULAR PLANT-MICROBE INTERACTIONS LA English DT Article ID BACTERIAL FRUIT ROT; CEPACIA COMPLEX; PLANT-GROWTH; BIOLOGICAL-CONTROL; PSEUDOMONAS-FLUORESCENS; ROOT COLONIZATION; DIVERSITY; STRAIN; CENOCEPACIA; VIRULENCE AB From a screen of 36 plant-associated strains of Burkholderia spp., we identified 24 strains that suppressed leaf and pseudobulb necrosis of orchid caused by B. gladioli. To gain insights into the mechanisms of disease suppression, we generated a draft genome sequence from one suppressive strain, TC3.4.2R3. The genome is an estimated 7.67 megabases in size, with three replicons, two chromosomes, and the plasmid pC3. Using a combination of multilocus sequence analysis and phylogenomics, we identified TC3.4.2R3 as B. seminalis, a species within the Burkholderia cepacia complex that includes opportunistic human pathogens and environmental strains. We generated and screened a library of 3,840 transposon mutants of strain TC3.4.2R3 on orchid leaves to identify genes contributing to plant disease suppression. Twelve mutants deficient in suppression of leaf necrosis were selected and the transposon insertions were mapped to eight loci. One gene is in a wcb cluster that is related to synthesis of extracellular polysaccharide, a key determinant in bacterial-host interactions in other systems, and the other seven are highly conserved among Burkholderia spp. The fundamental information developed in this study will serve as a resource for future research aiming to identify mechanisms contributing to biological control. C1 [Araujo, Welington L.; Mano, Emy T.; Camargo-Neves, Aline A.] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Lab Mol Biol & Microbial Ecol, Sao Paulo, Brazil. [Creason, Allison L.; Chang, Jeff H.; Loper, Joyce E.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Creason, Allison L.; Chang, Jeff H.; Loper, Joyce E.] Oregon State Univ, Mol & Cellular Biol Program, Corvallis, OR 97331 USA. [Camargo-Neves, Aline A.; Minami, Sonia N.] Univ Mogi Das Cruzes, Interdisciplinary Ctr Biotechnol, Mogi Das Cruzes, Brazil. [Chang, Jeff H.] Oregon State Univ, Ctr Genome Res & Biocomp, Corvallis, OR 97331 USA. [Loper, Joyce E.] USDA ARS, Hort Crops Lab, Corvallis, OR USA. RP Araujo, WL (reprint author), Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Lab Mol Biol & Microbial Ecol, Sao Paulo, Brazil. EM wlaraujo@usp.br OI Loper, Joyce/0000-0003-3501-5969 FU Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2012/24217-6, 2013/02124-9]; Fundacao de Amparo ao Ensino e Pesquisa (FAEP) (University of Mogi das Cruzes); Agriculture and Food Research Initiative Competitive Grants Program from the United States Department of Agriculture (USDA) National Institute of Food and Agriculture, National Science Foundation [2011-67019-30192, 2012-67013-19392, IOS-1021463]; National Institute of General Medical Sciences of the National Institutes of Health [RO1GM104977]; Agriculture and Food Research Initiative Competitive from the USDA National Institute of Food and Agriculture [2011-67019-30192] FX This work was supported by grant from Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) (2012/24217-6 and 2013/02124-9) and Fundacao de Amparo ao Ensino e Pesquisa (FAEP) (University of Mogi das Cruzes). Work in the J. H. Chang lab is supported in part by Agriculture and Food Research Initiative Competitive Grants Program (grant numbers 2011-67019-30192 and 2012-67013-19392) from the United States Department of Agriculture (USDA) National Institute of Food and Agriculture, National Science Foundation (grant number IOS-1021463), and National Institute of General Medical Sciences of the National Institutes of Health under award number RO1GM104977. Work in the J. E. Loper lab was supported in part by Agriculture and Food Research Initiative Competitive grant 2011-67019-30192 from the USDA National Institute of Food and Agriculture. NR 71 TC 1 Z9 1 U1 2 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0894-0282 EI 1943-7706 J9 MOL PLANT MICROBE IN JI Mol. Plant-Microbe Interact. PD JUN PY 2016 VL 29 IS 6 BP 435 EP 446 DI 10.1094/MPMI-02-16-0047-R PG 12 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences GA DN1GE UT WOS:000376813500001 PM 26959838 ER PT J AU Koenig, WD Liebhold, AM AF Koenig, Walter D. Liebhold, Andrew M. TI Temporally increasing spatial synchrony of North American temperature and bird populations SO NATURE CLIMATE CHANGE LA English DT Article ID ATLANTIC OSCILLATION; CLIMATE-CHANGE; CORRELATED ENVIRONMENTS; DYNAMICS; EXTINCTIONS; PERSISTENCE; EVENTS; RATES AB The ecological impacts of modern global climate change are detectable in a wide variety of phenomena, ranging from shifts in species ranges to changes in community composition and human disease dynamics(1-3). So far, however, little attention has been given to temporal changes in spatial synchrony-the coincident change in abundance or value across the landscape(4)-despite the importance of environmental synchrony as a driver of population trends and the central role of environmental variability in population rescue and extinction(1,5,6). Here we demonstrate that across North America, spatial synchrony of a significant proportion of 49 widespread North American wintering bird species has increased over the past 50 years-the period encompassing particularly intense anthropogenic effects in climate-paralleling significant increases in spatial synchrony of mean maximum air temperature. These results suggest the potential for increased spatial synchrony in environmental factors to be affecting a wide range of ecological phenomena. These effects are likely to vary, but for North American wildlife species, increased spatial synchrony driven by environmental factors may be the basis for a previously unrecognized threat to their long-term persistence in the form of more synchronized population dynamics reducing the potential for demographic rescue among interacting subpopulations. C1 [Koenig, Walter D.] Cornell Lab Ornithol, 159 Sapsucker Woods Rd, Ithaca, NY 14850 USA. [Koenig, Walter D.] Cornell Univ, Dept Neurobiol & Behav, Ithaca, NY 14853 USA. [Liebhold, Andrew M.] USDA Forest Serv, No Res Stn, 180 Canfield St, Morgantown, WV 26505 USA. RP Koenig, WD (reprint author), Cornell Lab Ornithol, 159 Sapsucker Woods Rd, Ithaca, NY 14850 USA.; Koenig, WD (reprint author), Cornell Univ, Dept Neurobiol & Behav, Ithaca, NY 14853 USA. EM wdk4@cornell.edu FU National Science Foundation [IOS-0918944, DEB-1256394] FX We thank A. Allstadt, J. Buonaccorsi, C. Cooper, A. Dhondt, W. Hochachka and B. Zuckerberg for comments, discussion and statistical advice. This work was funded by National Science Foundation grants IOS-0918944 and DEB-1256394 to W.D.K. NR 33 TC 1 Z9 1 U1 6 U2 10 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1758-678X EI 1758-6798 J9 NAT CLIM CHANGE JI Nat. Clim. Chang. PD JUN PY 2016 VL 6 IS 6 BP 614 EP + DI 10.1038/NCLIMATE2933 PG 5 WC Environmental Sciences; Environmental Studies; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DM6ZC UT WOS:000376500600018 ER PT J AU Okbay, A Baselmans, BML De Neve, JE Turley, P Nivard, MG Fontana, MA Meddens, SFW Linner, RK Rietveld, CA Derringer, J Gratten, J Lee, JJ Liu, JZ de Vlaming, R Ahluwalia, TS Buchwald, J Cavadino, A Frazier-Wood, AC Furlotte, NA Garfield, V Geisel, MH Gonzalez, JR Haitjema, S Karlsson, R van der Laan, SW Ladwig, KH Lahti, J van der Lee, SJ Lind, PA Liu, T Matteson, L Mihailov, E Miller, MB Minica, CC Nolte, IM Mook-Kanamori, D van der Most, PJ Oldmeadow, C Qian, Y Raitakari, O Rawal, R Realo, A Rueedi, R Schmidt, B Smith, AV Stergiakouli, E Tanaka, T Taylor, K Wedenoja, J Wellmann, J Westra, HJ Willems, SM Zhao, W Amin, N Bakshi, A Boyle, PA Cherney, S Cox, SR Davies, G Davis, OSP Ding, J Direk, N Eibich, P Emeny, RT Fatemifar, G Faul, JD Ferrucci, L Forstner, A Gieger, C Gupta, R Harris, TB Harris, JM Holliday, EG Hottenga, JJ De Jager, PL Kaakinen, MA Kajantie, E Karhunen, V Kolcic, I Kumari, M Launer, LJ Franke, L Li-Gao, R Koini, M Loukola, A Marques-Vidal, P Montgomery, GW Mosing, MA Paternoster, L Pattie, A Petrovic, KE Pulkki-Raback, L Quaye, L Raikkonen, K Rudan, I Scott, RJ Smith, JA Sutin, AR Trzaskowski, M Vinkhuyzen, AE Yu, L Zabaneh, D Attia, JR Bennett, DA Berger, K Bertram, L Boomsma, DI Snieder, H Chang, SC Cucca, F Deary, IJ van Duijn, CM Eriksson, JG Bultmann, U de Geus, EJC Groenen, PJF Gudnason, V Hansen, T Hartman, CA Haworth, CMA Hayward, C Heath, AC Hinds, DA Hypponen, E Iacono, WG Jarvelin, MR Jockel, KH Kaprio, J Kardia, SLR Keltikangas-Jarvinen, L Kraft, P Kubzansky, LD Lehtimaki, T Magnusson, PKE Martin, NG Mcgue, M Metspalu, A Mills, M de Mutsert, R Oldehinkel, AJ Pasterkamp, G Pedersen, NL Plomin, R Polasek, O Power, C Rich, SS Rosendaal, FR den Ruijter, HM Schlessinger, D Schmidt, H Svento, R Schmidt, R Alizadeh, BZ Sorensen, TIA Spector, TD Steptoe, A Terracciano, A Thurik, AR Timpson, NJ Tiemeier, H Uitterlinden, AG Vollenweider, P Wagner, GG Weir, DR Yang, J Conley, DC Smith, GD Hofman, A Johannesson, M Laibson, DI Medland, SE Meyer, MN Pickrell, JK Esko, T Krueger, RF Beauchamp, JP Koellinger, PD Benjamin, DJ Bartels, M Cesarini, D AF Okbay, Aysu Baselmans, Bart M. L. De Neve, Jan-Emmanuel Turley, Patrick Nivard, Michel G. Fontana, Mark Alan Meddens, S. Fleur W. Linner, Richard Karlsson Rietveld, Cornelius A. Derringer, Jaime Gratten, Jacob Lee, James J. Liu, Jimmy Z. de Vlaming, Ronald Ahluwalia, Tarunveer S. Buchwald, Jadwiga Cavadino, Alana Frazier-Wood, Alexis C. Furlotte, Nicholas A. Garfield, Victoria Geisel, Marie Henrike Gonzalez, Juan R. Haitjema, Saskia Karlsson, Robert van der Laan, Sander W. Ladwig, Karl-Heinz Lahti, Jari van der Lee, Sven J. Lind, Penelope A. Liu, Tian Matteson, Lindsay Mihailov, Evelin Miller, Michael B. Minica, Camelia C. Nolte, Ilja M. Mook-Kanamori, Dennis van der Most, Peter J. Oldmeadow, Christopher Qian, Yong Raitakari, Olli Rawal, Rajesh Realo, Anu Rueedi, Rico Schmidt, Borge Smith, Albert V. Stergiakouli, Evie Tanaka, Toshiko Taylor, Kent Wedenoja, Juho Wellmann, Juergen Westra, Harm-Jan Willems, Sara M. Zhao, Wei Amin, Najaf Bakshi, Andrew Boyle, Patricia A. Cherney, Samantha Cox, Simon R. Davies, Gail Davis, Oliver S. P. Ding, Jun Direk, Nese Eibich, Peter Emeny, Rebecca T. Fatemifar, Ghazaleh Faul, Jessica D. Ferrucci, Luigi Forstner, Andreas Gieger, Christian Gupta, Richa Harris, Tamara B. Harris, Juliette M. Holliday, Elizabeth G. Hottenga, Jouke-Jan De Jager, Philip L. Kaakinen, Marika A. Kajantie, Eero Karhunen, Ville Kolcic, Ivana Kumari, Meena Launer, Lenore J. Franke, Lude Li-Gao, Ruifang Koini, Marisa Loukola, Anu Marques-Vidal, Pedro Montgomery, Grant W. Mosing, Miriam A. Paternoster, Lavinia Pattie, Alison Petrovic, Katja E. Pulkki-Raback, Laura Quaye, Lydia Raikkonen, Katri Rudan, Igor Scott, Rodney J. Smith, Jennifer A. Sutin, Angelina R. Trzaskowski, Maciej Vinkhuyzen, Anna E. Yu, Lei Zabaneh, Delilah Attia, John R. Bennett, David A. Berger, Klaus Bertram, Lars Boomsma, Dorret I. Snieder, Harold Chang, Shun-Chiao Cucca, Francesco Deary, Ian J. van Duijn, Cornelia M. Eriksson, Johan G. Bultmann, Ute de Geus, Eco J. C. Groenen, Patrick J. F. Gudnason, Vilmundur Hansen, Torben Hartman, Catharine A. Haworth, Claire M. A. Hayward, Caroline Heath, Andrew C. Hinds, David A. Hypponen, Elina Iacono, William G. Jarvelin, Marjo-Riitta Jockel, Karl-Heinz Kaprio, Jaakko Kardia, Sharon L. R. Keltikangas-Jarvinen, Liisa Kraft, Peter Kubzansky, Laura D. Lehtimaki, Terho Magnusson, Patrik K. E. Martin, Nicholas G. Mcgue, Matt Metspalu, Andres Mills, Melinda de Mutsert, Renee Oldehinkel, Albertine J. Pasterkamp, Gerard Pedersen, Nancy L. Plomin, Robert Polasek, Ozren Power, Christine Rich, Stephen S. Rosendaal, Frits R. den Ruijter, Hester M. Schlessinger, David Schmidt, Helena Svento, Rauli Schmidt, Reinhold Alizadeh, Behrooz Z. Sorensen, Thorkild I. A. Spector, Tim D. Steptoe, Andrew Terracciano, Antonio Thurik, A. Roy Timpson, Nicholas J. Tiemeier, Henning Uitterlinden, Andre G. Vollenweider, Peter Wagner, Gert G. Weir, David R. Yang, Jian Conley, Dalton C. Smith, George Davey Hofman, Albert Johannesson, Magnus Laibson, David I. Medland, Sarah E. Meyer, Michelle N. Pickrell, Joseph K. Esko, Tonu Krueger, Robert F. Beauchamp, Jonathan P. Koellinger, Philipp D. Benjamin, Daniel J. Bartels, Meike Cesarini, David CA LifeLines Cohort Study TI Genetic variants associated with subjective well-being, depressive symptoms, and neuroticism identified through genome-wide analyses SO NATURE GENETICS LA English DT Article ID MAJOR DEPRESSION; COMMON VARIANTS; HUMAN HEIGHT; RISK; HERITABILITY; METAANALYSIS; PERSONALITY; HAPPINESS; RESOURCE; DISEASE AB Very few genetic variants have been associated with depression and neuroticism, likely because of limitations on sample size in previous studies. Subjective well-being, a phenotype that is genetically correlated with both of these traits, has not yet been studied with genome-wide data. We conducted genome-wide association studies of three phenotypes: subjective well-being (n = 298,420), depressive symptoms (n = 161,460), and neuroticism (n = 170,911). We identify 3 variants associated with subjective well-being, 2 variants associated with depressive symptoms, and 11 variants associated with neuroticism, including 2 inversion polymorphisms. The two loci associated with depressive symptoms replicate in an independent depression sample. Joint analyses that exploit the high genetic correlations between the phenotypes (vertical bar(p) over cap vertical bar approximate to 0.8) strengthen the overall credibility of the findings and allow us to identify additional variants. Across our phenotypes, loci regulating expression in central nervous system and adrenal or pancreas tissues are strongly enriched for association. C1 [Okbay, Aysu; Rietveld, Cornelius A.; de Vlaming, Ronald; Thurik, A. Roy] Erasmus Univ, Erasmus Sch Econ, Dept Appl Econ, Rotterdam, Netherlands. [Okbay, Aysu; Rietveld, Cornelius A.; de Vlaming, Ronald; van der Lee, Sven J.; Willems, Sara M.; Amin, Najaf; van Duijn, Cornelia M.; Tiemeier, Henning; Uitterlinden, Andre G.; Hofman, Albert] Erasmus MC, Dept Epidemiol, Rotterdam, Netherlands. [Okbay, Aysu; Meddens, S. Fleur W.; Linner, Richard Karlsson; Rietveld, Cornelius A.; de Vlaming, Ronald; Groenen, Patrick J. F.; Thurik, A. Roy; Uitterlinden, Andre G.; Koellinger, Philipp D.] Erasmus Univ, Inst Behav & Biol, Rotterdam, Netherlands. [Baselmans, Bart M. L.; Nivard, Michel G.; Minica, Camelia C.; Hottenga, Jouke-Jan; Boomsma, Dorret I.; de Geus, Eco J. C.; Bartels, Meike] Vrije Univ Amsterdam, Dept Biol Psychol, Amsterdam, Netherlands. [Baselmans, Bart M. L.; Hottenga, Jouke-Jan; Boomsma, Dorret I.; de Geus, Eco J. C.; Bartels, Meike] EMGO Inst Hlth & Care Res, Amsterdam, Netherlands. [De Neve, Jan-Emmanuel] Univ Oxford, Said Business Sch, Oxford, England. [Turley, Patrick; Laibson, David I.; Beauchamp, Jonathan P.] Harvard Univ, Dept Econ, Cambridge, MA 02138 USA. [Fontana, Mark Alan; Benjamin, Daniel J.] Univ So Calif, Ctr Econ & Social Res, Los Angeles, CA USA. [Meddens, S. Fleur W.; Linner, Richard Karlsson; Koellinger, Philipp D.] Vrije Univ, Ctr Neurogenom & Cognit Res, Dept Complex Trait Genet, Amsterdam, Netherlands. [Meddens, S. Fleur W.; Linner, Richard Karlsson; Koellinger, Philipp D.] Univ Amsterdam, Amsterdam Business Sch, Amsterdam, Netherlands. [Derringer, Jaime] Univ Illinois, Dept Psychol, Champaign, IL USA. [Gratten, Jacob; Bakshi, Andrew; Trzaskowski, Maciej; Vinkhuyzen, Anna E.; Yang, Jian] Univ Queensland, Queensland Brain Inst, Brisbane, Qld, Australia. [Lee, James J.; Matteson, Lindsay; Miller, Michael B.; Iacono, William G.; Mcgue, Matt; Krueger, Robert F.] Univ Minnesota Twin Cities, Dept Psychol, Minneapolis, MN USA. [Liu, Jimmy Z.; Pickrell, Joseph K.] New York Genome Ctr, New York, NY USA. [Ahluwalia, Tarunveer S.] Univ Copenhagen, Herlev & Gentofte Hosp, COPSAC Copenhagen Prospect Studies Asthma Childho, Copenhagen, Denmark. [Ahluwalia, Tarunveer S.; Hansen, Torben; Sorensen, Thorkild I. A.] Univ Copenhagen, Fac Med & Hlth Sci, Sect Metab Genet, Novo Nordisk Fdn Ctr Basic Metab Res, Copenhagen, Denmark. [Ahluwalia, Tarunveer S.] Steno Diabet Ctr, Gentofte, Denmark. [Buchwald, Jadwiga; Wedenoja, Juho; Gupta, Richa; Loukola, Anu; Kaprio, Jaakko] Univ Helsinki, Dept Publ Hlth, Helsinki, Finland. [Cavadino, Alana] Queen Mary Univ London, Wolfson Inst Prevent Med, Ctr Environm & Prevent Med, London, England. [Cavadino, Alana; Hypponen, Elina; Power, Christine] South Australian Hlth & Med Res Inst, Adelaide, SA, Australia. [Frazier-Wood, Alexis C.] Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Furlotte, Nicholas A.; Hinds, David A.] 23andMe Inc, Mountain View, CA USA. [Garfield, Victoria; Steptoe, Andrew] UCL, Dept Epidemiol & Publ Hlth, London, England. [Geisel, Marie Henrike; Schmidt, Borge; Jockel, Karl-Heinz] Univ Hosp Essen, Inst Med Informat Biometry & Epidemiol, Essen, Germany. [Gonzalez, Juan R.] Inst Global Hlth, Ctr Res Environm Epidemiol, Barcelona, Spain. [Gonzalez, Juan R.] Univ Pompeu Fabra, Barcelona, Spain. [Gonzalez, Juan R.] CIBER Epidemiol & Salud Publ, Barcelona, Spain. [Haitjema, Saskia; van der Laan, Sander W.; Pasterkamp, Gerard] Univ Med Ctr Utrecht, Lab Expt Cardiol, Utrecht, Netherlands. [Karlsson, Robert; Magnusson, Patrik K. E.; Pedersen, Nancy L.] Karolinska Inst, Dept Med Epidemiol & Biostat, Stockholm, Sweden. [Ladwig, Karl-Heinz; Emeny, Rebecca T.] Helmholtz Zentrum Munchen German Res Ctr Environ, Mental Hlth Res Unit, Inst Epidemiol 2, Neuherberg, Germany. [Ladwig, Karl-Heinz] Tech Univ Munich, Klinikum Rechts Isar, Dept Psychosomat Med & Psychotherapy, D-80290 Munich, Germany. [Lahti, Jari; Pulkki-Raback, Laura; Raikkonen, Katri; Keltikangas-Jarvinen, Liisa] Univ Helsinki, Inst Behav Sci, Helsinki, Finland. [Lahti, Jari] Folkhalsan Res Ctr, Helsinki, Finland. [Lahti, Jari; Pulkki-Raback, Laura] Univ Helsinki, Helsinki Collegium Adv Studies, Helsinki, Finland. [Lind, Penelope A.; Medland, Sarah E.] QIMR Berghofer Med Res Inst, Psychiat Genet, Brisbane, Qld, Australia. [Liu, Tian; Wagner, Gert G.] Max Planck Inst Human Dev, Berlin, Germany. [Liu, Tian] Max Planck Inst Mol Genet, Dept Vertebrate Gen, Ihnestr 73, D-14195 Berlin, Germany. [Mihailov, Evelin; Metspalu, Andres; Esko, Tonu] Univ Tartu, Estonian Genome Ctr, Ulikooli 18, EE-50090 Tartu, Estonia. [Nolte, Ilja M.; van der Most, Peter J.; Snieder, Harold; Alizadeh, Behrooz Z.] Univ Groningen, Univ Med Ctr Groningen, Dept Epidemiol, Groningen, Netherlands. [Mook-Kanamori, Dennis; Li-Gao, Ruifang; de Mutsert, Renee; Rosendaal, Frits R.] Leiden Univ, Med Ctr, Clin Epidemiol, Leiden, Netherlands. [Mook-Kanamori, Dennis] Leiden Univ, Med Ctr, Publ Hlth & Primary Care, Leiden, Netherlands. [Mook-Kanamori, Dennis] King Faisal Specialist Hosp & Res Ctr, BESC, Riyadh 11211, Saudi Arabia. [Oldmeadow, Christopher; Holliday, Elizabeth G.; Attia, John R.] Hunter Med Res Inst, Publ Hlth Stream, New Lambton, NSW, Australia. [Oldmeadow, Christopher; Holliday, Elizabeth G.; Scott, Rodney J.; Attia, John R.] Univ Newcastle, Fac Hlth & Med, Newcastle, NSW 2300, Australia. [Qian, Yong; Ding, Jun; Schlessinger, David] NIA, Genet Lab, Baltimore, MD 21224 USA. [Raitakari, Olli] Univ Turku, Res Ctr Appl & Prevent Cardiovasc Med, Turku, Finland. [Raitakari, Olli] Turku Univ Hosp, Dept Clin Physiol, FIN-20520 Turku, Finland. [Rawal, Rajesh; Gieger, Christian] Helmholtz Zentrum Munchen German Res Ctr Environ, Res Unit Mol Epidemiol, Neuherberg, Germany. [Realo, Anu] Univ Tartu, Dept Psychol, Ulikooli 18, EE-50090 Tartu, Estonia. 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[Groenen, Patrick J. F.; Oldehinkel, Albertine J.] Erasmus Univ, Inst Econometr, Erasmus Sch Econ, POB 1738, NL-3000 DR Rotterdam, Netherlands. [Hartman, Catharine A.] Univ Groningen, Univ Med Ctr Groningen, Dept Psychiat, Groningen, Netherlands. [Hayward, Caroline] Univ Edinburgh, Inst Genet & Mol Med, MRC Human Genet Unit, Edinburgh, Midlothian, Scotland. [Heath, Andrew C.] Washington Univ, Sch Med, Dept Psychiat, St Louis, MO 63110 USA. [Hypponen, Elina] Univ S Australia, Sch Hlth Sci, Ctr Populat Hlth Res, Adelaide, SA 5001, Australia. [Hypponen, Elina] Univ S Australia, Sansom Inst, Adelaide, SA 5001, Australia. [Hypponen, Elina; Power, Christine] UCL Inst Child Hlth, Populat Policy & Practice, London, England. [Jarvelin, Marjo-Riitta] Univ London Imperial Coll Sci Technol & Med, Sch Publ Hlth, MRC PHE Ctr Environm & Hlth, Dept Epidemiol & Biostat, London, England. [Jarvelin, Marjo-Riitta] Univ Oulu, Bioctr Oulu, Oulu, Finland. [Jarvelin, Marjo-Riitta] Oulu Univ Hosp, Unit Primary Care, Oulu, Finland. [Kaprio, Jaakko] Univ Helsinki, Inst Mol Med Finland FIMM, Helsinki, Finland. [Kaprio, Jaakko] THL Natl Inst Hlth & Welf, Dept Hlth, Helsinki, Finland. [Kraft, Peter] Harvard Univ, TH Chan Sch Publ Hlth, Dept Epidemiol & Biostat, Boston, MA 02115 USA. [Kubzansky, Laura D.] Harvard Univ, TH Chan Sch Publ Hlth, Dept Social & Behav Sci, Boston, MA 02115 USA. [Lehtimaki, Terho] Fimlab Labs, Tampere, Finland. [Lehtimaki, Terho] Univ Tampere, Sch Med, Dept Clin Chem, FIN-33101 Tampere, Finland. [Martin, Nicholas G.] QIMR Berghofer Med Res Inst, Genet Epidemiol, Brisbane, Qld, Australia. [Metspalu, Andres] Univ Tartu, Inst Mol & Cell Biol, Ulikooli 18, EE-50090 Tartu, Estonia. [Mills, Melinda] Univ Oxford, Dept Sociol, Oxford, England. [Pasterkamp, Gerard] Univ Med Ctr Utrecht, Div Labs & Pharm, Lab Clin Chem & Hematol, Utrecht, Netherlands. [Plomin, Robert] Kings Coll London, Social Genet & Dev Psychiat Ctr, De Crespigny Pk, London, England. [Rich, Stephen S.] Univ Virginia, Dept Publ Hlth Sci, Charlottesville, VA USA. [Schmidt, Helena] Gen Hosp, Ctr Mol Med, Res Unit Genet Epidemiol, Inst Mol Biol & Biochem, Graz, Austria. [Schmidt, Helena] Med Univ Graz, Graz, Austria. [Svento, Rauli] Oulu Business Sch, Dept Econ, Oulu, Finland. [Alizadeh, Behrooz Z.] Univ Groningen, Univ Med Ctr Groningen, Dept Gastroenterol & Hepatol, Groningen, Netherlands. [Sorensen, Thorkild I. A.] Bispebjerg & Frederiksberg Hosp, Inst Prevent Med, Frederiksberg, Denmark. [Thurik, A. Roy] Montpellier Business Sch, Montpellier, France. [Thurik, A. Roy] Panteia, Zoetermeer, Netherlands. [Tiemeier, Henning] Erasmus MC, Dept Psychiat, Rotterdam, Netherlands. [Tiemeier, Henning] Erasmus MC, Dept Child & Adolescent Psychiat, Rotterdam, Netherlands. [Uitterlinden, Andre G.] Erasmus MC, Dept Internal Med, Rotterdam, Netherlands. [Wagner, Gert G.] Berlin Univ Technol, Sch Econ & Management, Berlin, Germany. [Yang, Jian] Univ Queensland Diamantina Inst, Translat Res Inst, Brisbane, Qld, Australia. [Conley, Dalton C.] Princeton Univ, Dept Sociol, Princeton, NJ 08544 USA. [Hofman, Albert] Harvard Univ, TH Chan Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA. [Johannesson, Magnus] Stockholm Sch Econ, Dept Econ, S-11383 Stockholm, Sweden. [Meyer, Michelle N.] Clarkson Univ, Dept Bioeth, Schenectady, NY USA. [Meyer, Michelle N.] Icahn Sch Med Mt Sinai, New York, NY 10029 USA. [Pickrell, Joseph K.] Columbia Univ, Dept Biol Sci, New York, NY 10027 USA. [Cesarini, David] NYU, Dept Econ, New York, NY 10003 USA. [Cesarini, David] Res Inst Ind Econ, Stockholm, Sweden. RP Koellinger, PD (reprint author), Erasmus Univ, Inst Behav & Biol, Rotterdam, Netherlands.; Bartels, M (reprint author), Vrije Univ Amsterdam, Dept Biol Psychol, Amsterdam, Netherlands.; Bartels, M (reprint author), EMGO Inst Hlth & Care Res, Amsterdam, Netherlands.; Benjamin, DJ (reprint author), Univ So Calif, Ctr Econ & Social Res, Los Angeles, CA USA.; Koellinger, PD (reprint author), Vrije Univ, Ctr Neurogenom & Cognit Res, Dept Complex Trait Genet, Amsterdam, Netherlands.; Koellinger, PD (reprint author), Univ Amsterdam, Amsterdam Business Sch, Amsterdam, Netherlands.; Bartels, M (reprint author), Neurosci Campus Amsterdam, Amsterdam, Netherlands. EM p.d.koellinger@vu.nl; djbenjam@usc.edu; m.bartels@vu.nl RI Polasek, Ozren/B-6002-2011; Kolcic, Ivana/E-2713-2017; Thurik, Roy/A-9386-2012; Davey Smith, George/A-7407-2013; Magnusson, Patrik/C-4458-2017; Ladwig, Karl-Heinz/B-5351-2014; Yang, Jian/A-5852-2010; Johannesson, Magnus/E-9680-2011; Haworth, Claire/C-7073-2009; Realo, Anu/M-9524-2016; Bartels, Meike/D-4492-2014; Franke, Lude/P-7036-2016; Colaus, PsyColaus/K-6607-2013; Bertram, Lars/K-3889-2015; Gratten, Jake (Jacob)/G-1485-2011 OI Medland, Sarah/0000-0003-1382-380X; Timpson, Nicholas/0000-0002-7141-9189; Lahti, Jari/0000-0002-4310-5297; Wedenoja, Juho/0000-0002-6155-0378; Bakshi, Andrew/0000-0001-5650-7036; Hypponen, Elina/0000-0003-3670-9399; Trzaskowski, Maciej/0000-0001-8559-6525; Nivard, Michel/0000-0003-2015-1888; Stergiakouli, Evangelia/0000-0003-3586-0927; Kaprio, Jaakko/0000-0002-3716-2455; Smith, Jennifer/0000-0002-3575-5468; Polasek, Ozren/0000-0002-5765-1862; Kolcic, Ivana/0000-0001-7918-6052; Gupta, Richa/0000-0001-7700-5828; Cesarini, David/0000-0002-0043-009X; Gieger, Christian/0000-0001-6986-9554; Buchwald, Jadwiga/0000-0001-6688-8346; Loukola, Anu-Maria/0000-0003-0542-5967; Kumari, Meena/0000-0001-9716-1035; Thurik, Roy/0000-0002-0242-6908; Davey Smith, George/0000-0002-1407-8314; Ladwig, Karl-Heinz/0000-0003-0710-1720; Yang, Jian/0000-0003-2001-2474; Johannesson, Magnus/0000-0001-8759-6393; Haworth, Claire/0000-0002-8608-289X; Bartels, Meike/0000-0002-9667-7555; Franke, Lude/0000-0002-5159-8802; Bertram, Lars/0000-0002-0108-124X; Gratten, Jake (Jacob)/0000-0003-1293-409X FU Social Science Genetic Association Consortium (SSGAC); US National Science Foundation (EAGER: 'Workshop for the Formation of a Social Science Genetic Association Consortium'); National Institute of Health Office of Behavioral and Social Science Research; Ragnar Soderberg Foundation [E9/11]; Swedish Research Council [421-2013-1061]; Jan Wallander and Tom Hedelius Foundation; ERC Consolidator Grant [647648 EdGe]; Pershing Square Fund of the Foundations of Human Behavior; NIA/NIH [P01-AG005842, P01-AG005842-20S2, P30-AG012810, T32-AG000186-23, R01-AG042568-02] FX We are grateful to P.M. Visscher for advice, support, and feedback. We thank S. Cunningham and N. Galla for research assistance. This research was carried out under the auspices of the Social Science Genetic Association Consortium (SSGAC). The SSGAC seeks to facilitate studies that investigate the influence of genes on human behavior, well-being, and social-scientific outcomes using large GWAS meta-analyses. The SSGAC also provides opportunities for replication and promotes the collection of accurately measured, harmonized phenotypes across cohorts. The SSGAC operates as a working group within the CHARGE Consortium. This research has also been conducted using the UK Biobank Resource. The study was supported by funding from the US National Science Foundation (EAGER: 'Workshop for the Formation of a Social Science Genetic Association Consortium'), a supplementary grant from the National Institute of Health Office of Behavioral and Social Science Research, the Ragnar Soderberg Foundation (E9/11), the Swedish Research Council (421-2013-1061), the Jan Wallander and Tom Hedelius Foundation, an ERC Consolidator Grant (647648 EdGe), the Pershing Square Fund of the Foundations of Human Behavior, and the NIA/NIH through grants P01-AG005842, P01-AG005842-20S2, P30-AG012810, and T32-AG000186-23 to NBER and R01-AG042568-02 to the University of Southern California. A full list of acknowledgments is provided in the Supplementary Note. NR 47 TC 26 Z9 26 U1 18 U2 39 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1061-4036 EI 1546-1718 J9 NAT GENET JI Nature Genet. PD JUN PY 2016 VL 48 IS 6 BP 624 EP + DI 10.1038/ng.3552 PG 13 WC Genetics & Heredity SC Genetics & Heredity GA DN0HE UT WOS:000376744200009 PM 27089181 ER PT J AU Iorizzo, M Ellison, S Senalik, D Zeng, P Satapoomin, P Huang, JY Bowman, M Iovene, M Sanseverino, W Cavagnaro, P Yildiz, M Macko-Podgorni, A Moranska, E Grzebelus, E Grzebelus, D Ashrafi, H Zheng, ZJ Cheng, SF Spooner, D Van Deynze, A Simon, P AF Iorizzo, Massimo Ellison, Shelby Senalik, Douglas Zeng, Peng Satapoomin, Pimchanok Huang, Jiaying Bowman, Megan Iovene, Marina Sanseverino, Walter Cavagnaro, Pablo Yildiz, Mehtap Macko-Podgorni, Alicja Moranska, Emilia Grzebelus, Ewa Grzebelus, Dariusz Ashrafi, Hamid Zheng, Zhijun Cheng, Shifeng Spooner, David Van Deynze, Allen Simon, Philipp TI A high-quality carrot genome assembly provides new insights into carotenoid accumulation and asterid genome evolution SO NATURE GENETICS LA English DT Article ID 1-DEOXY-D-XYLULOSE 5-PHOSPHATE SYNTHASE; DAUCUS-CAROTA; FLOWERING TIME; TRANSPOSABLE ELEMENTS; POPULATION-STRUCTURE; TRANSCRIPTION FACTOR; MAXIMUM-LIKELIHOOD; ANALYSIS TOOLKIT; SEQUENCING DATA; GENE AB We report a high-quality chromosome-scale assembly and analysis of the carrot (Daucus carota) genome, the first sequenced genome to include a comparative evolutionary analysis among members of the euasterid II clade. We characterized two new polyploidization events, both occurring after the divergence of carrot from members of the Asterales order, clarifying the evolutionary scenario before and after radiation of the two main asterid clades. Large-and small-scale lineage-specific duplications have contributed to the expansion of gene families, including those with roles in flowering time, defense response, flavor, and pigment accumulation. We identified a candidate gene, DCAR_032551, that conditions carotenoid accumulation (Y) in carrot taproot and is coexpressed with several isoprenoid biosynthetic genes. The primary mechanism regulating carotenoid accumulation in carrot taproot is not at the biosynthetic level. We hypothesize that DCAR_032551 regulates upstream photosystem development and functional processes, including photomorphogenesis and root de-etiolation. C1 [Iorizzo, Massimo; Ellison, Shelby; Senalik, Douglas; Satapoomin, Pimchanok; Spooner, David; Simon, Philipp] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Senalik, Douglas; Spooner, David; Simon, Philipp] USDA ARS, Vegetable Crops Res Unit, Madison, WI USA. [Zeng, Peng; Huang, Jiaying; Zheng, Zhijun; Cheng, Shifeng] Beijing Genom Inst Shenzhen, Shenzhen, Peoples R China. [Bowman, Megan] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. [Iovene, Marina] CNR, Inst Biosci & Bioresources, Bari, Italy. [Sanseverino, Walter] Sequentia Biotech, Barcelona, Spain. [Cavagnaro, Pablo] Univ Nacl Cuyo, Fac Ciencias Agr, Natl Sci & Tech Res Council CONICET, Cuyo, Argentina. [Cavagnaro, Pablo] Inst Nacl Tecnol Agr, Estn Expt Agr Consulta, La Consulta, Argentina. [Yildiz, Mehtap] Yuzuncu Yil Univ, Fac Agr, Dept Agr Biotechnol, Van, Turkey. [Macko-Podgorni, Alicja; Moranska, Emilia; Grzebelus, Ewa; Grzebelus, Dariusz] Agr Univ Krakow, Inst Plant Biol & Biotechnol, Krakow, Poland. [Ashrafi, Hamid; Van Deynze, Allen] Univ Calif Davis, Seed Biotechnol Ctr, Davis, CA 95616 USA. [Iorizzo, Massimo; Ashrafi, Hamid] N Carolina State Univ, Dept Hort Sci, Plants Human Hlth Inst, Kannapolis, NC USA. [Ashrafi, Hamid] N Carolina State Univ, Dept Hort Sci, Raleigh, NC 27695 USA. RP Simon, P (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA.; Simon, P (reprint author), USDA ARS, Vegetable Crops Res Unit, Madison, WI USA. EM philipp.simon@ars.usda.gov OI Senalik, Douglas/0000-0001-8526-0554; Grzebelus, Dariusz/0000-0001-6999-913X; Bowman, Megan/0000-0001-5742-1779; Sanseverino, Walter/0000-0003-3324-5912 FU carrot industry; Bejo; Carosem; Monsanto; Nunhems; |Sumika; Takii; Vilmorin; National Science Foundation [1202666]; projects RGV-FAO [D.M.3824]; Polish National Science Center [2012/05/B/NZ9/03401]; Polish Ministry of Science and Higher Education to Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow; [PONa3_00025-BIOforIU] FX The authors appreciate the financial support of the carrot industry and the following vegetable seed companies-Bejo, Carosem, Monsanto, Nunhems, Rijk Zwaan (RZ), Sumika, Takii, and Vilmorin-with additional thanks to RZ for providing DH1, BAC libraries, and BES. S.E. was supported by the National Science Foundation under grant 1202666. M. Iovene thanks the projects RGV-FAO (D.M.3824) for partial financial support and PONa3_00025-BIOforIU for funding the acquisition of a fluorescence microscope. A.M.-P., E.M., E.G., and D.G. were supported by the Polish National Science Center, project 2012/05/B/NZ9/03401, and the statutory funds for science granted by the Polish Ministry of Science and Higher Education to the Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow. The authors thank H. Ruess for assembly and annotation of the plastid genome and R. Kane for support in the development of plant materials. NR 129 TC 10 Z9 10 U1 15 U2 28 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1061-4036 EI 1546-1718 J9 NAT GENET JI Nature Genet. PD JUN PY 2016 VL 48 IS 6 BP 657 EP + DI 10.1038/ng.3565 PG 14 WC Genetics & Heredity SC Genetics & Heredity GA DN0HE UT WOS:000376744200014 PM 27158781 ER PT J AU Sundaram, S Yan, L AF Sundaram, Sneha Yan, Lin TI Time-restricted feeding reduces adiposity in mice fed a high-fat diet SO NUTRITION RESEARCH LA English DT Article DE Time-restricted feeding; Ad libitum; High-fat diet; Adiposity; Mice ID MONOCYTE CHEMOATTRACTANT PROTEIN-1; ENDOTHELIAL GROWTH-FACTOR; MATRIX METALLOPROTEINASES; INSULIN-RESISTANCE; WEIGHT-LOSS; OBESITY; TISSUE; HUMANS; ADIPOGENESIS; INFLAMMATION AB Disruption of the circadian rhythm contributes to obesity. This study tested the hypothesis that time-restricted feeding (TRF) reduces high-fat diet-induced increase in adiposity. Male C57BL/6 mice were fed the AIN93G or the high-fat diet ad libitum (ad lib); TRF of the high-fat diet for 12 or 8 hours during the dark cycle was initiated when high-fat diet-fed mice exhibited significant increases in body weight. Energy intake of the TRF 12-hour group was not different from that of the high-fat ad lib group, although that of the TRF 8-hour group was slightly but significantly lower. Restricted feeding of the high-fat diet reduced body fat mass and body weight compared with mice fed the high-fat diet ad lib. There were no differences in respiratory exchange ratio (RER) among TRF and high-fat ad lib groups, but the RER of these groups was lower than that of the AIN93G group. Energy expenditure of the TRF groups was slightly but significantly lower than that of the high-fat ad lib group. Plasma concentrations of ghrelin were increased in TRF groups compared with both AIN93G and high-fat ad lib groups. Elevations of plasma concentrations of insulin, leptin, monocyte chemoattractant protein-1, and tissue inhibitor metalloproteinase-1 by high-fat ad lib feeding were reduced by TRF to the levels of mice fed the AIN93G diet. In conclusion, TRF during the dark cycle reduces high-fat diet-induced increases in adiposity and proinflammatory cytokines. These results indicate that circadian timing of food intake may prevent obesity and abate obesity-related metabolic disturbance. Published by Elsevier Inc. C1 [Sundaram, Sneha; Yan, Lin] USDA ARS, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave North, Grand Forks, ND 58202 USA. RP Yan, L (reprint author), USDA ARS, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave North, Grand Forks, ND 58202 USA. EM sneha.sundaram@ars.usda.gov; lin.yan@ars.usda.gov FU USDA, ARS, Research Project [3062-51000-050-00D] FX The authors gratefully acknowledge the assistance of the following staff of the Grand Forks Human Nutrition Research Center: Lana DeMars and Kay Keehr for technical support, LuAnn Johnson for statistical analysis, and James Lindlauf for preparing experimental diets and vivarium staff for providing high-quality animal care. The US Department of Agriculture (USDA), Agricultural Research Service, Plains Area, is an equal opportunity/affirmative action employer and all agency services are available without discrimination. Mention of trade names or commercial products in this article is solely for providing specific information and does not imply recommendation or endorsement by the USDA. Funding for this work was provided by the USDA, ARS, Research Project 3062-51000-050-00D. NR 42 TC 4 Z9 4 U1 8 U2 10 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0271-5317 J9 NUTR RES JI Nutr. Res. PD JUN PY 2016 VL 36 IS 6 BP 603 EP 611 DI 10.1016/j.nutres.2016.02.005 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DM9VA UT WOS:000376712600012 PM 27188906 ER PT J AU McCluskey, K Alvarez, A Bennett, R Bokati, D Boundy-Mills, K Brown, D Bull, CT Coffey, M Dreaden, T Duke, C Dye, G Ehmke, E Eversole, K Fenstermacher, K Geiser, D Glaeser, JA Greene, S Gribble, L Griffith, MP Hanser, K Humber, R Johnson, BW Kermode, A Krichevsky, M Laudon, M Leach, J Leslie, J May, M Melcher, U Nobles, D Fonseca, NR Robinson, S Ryan, M Scott, J Silflow, C Vidaver, A Webb, KM Wertz, JE Yentsch, S Zehr, S AF McCluskey, Kevin Alvarez, Anne Bennett, Rick Bokati, Deepak Boundy-Mills, Kyria Brown, Daniel Bull, Carolee T. Coffey, Michael Dreaden, Tyler Duke, Clifford Dye, Greg Ehmke, Erin Eversole, Kellye Fenstermacher, Kristi Geiser, David Glaeser, Jessie A. Greene, Stephanie Gribble, Lisa Griffith, M. Patrick Hanser, Kathryn Humber, Richard Johnson, Barbara W. Kermode, Anthony Krichevsky, Micah Laudon, Matt Leach, Jan Leslie, John May, Meghan Melcher, Ulrich Nobles, David Fonseca, Natalia Risso Robinson, Sara Ryan, Matthew Scott, James Silflow, Carolyn Vidaver, Anne Webb, Kimberly M. Wertz, John E. Yentsch, Sara Zehr, Sarah TI The US Culture Collection Network Lays the Foundation for Progress in Preservation of Valuable Microbial Resources SO PHYTOPATHOLOGY LA English DT Review ID PLANT PATHOLOGY; DIVERSITY; STRAINS; FUNGUS; FUTURE; RESISTANCE; BENEFITS; FUSARIUM; L. AB The U.S. Culture Collection Network was formed in 2012 by a group of culture collection scientists and stakeholders in order to continue the progress established previously through efforts of an ad hoc group. The network is supported by a Research Coordination Network grant from the U.S. National Science Foundation (NSF) and has the goals of promoting interaction among collections, encouraging the adoption of best practices, and protecting endangered or orphaned collections. After prior meetings to discuss best practices, shared data, and synergy with genome programs, the network held a meeting at the U.S. Department of Agriculture (USDA)-Agricultural Research Service (ARS) National Center for Genetic Resources Preservation (NCGRP) in Fort Collins, Colorado in October 2015 specifically to discuss collections that are vulnerable because of changes in funding programs, or are at risk of loss because of retirement or lack of funding. The meeting allowed collection curators who had already backed up their resources at the USDA NCGRP to visit the site, and brought collection owners, managers, and stakeholders together. Eight formal collections have established off-site backups with the USDA-ARS, ensuring that key material will be preserved for future research. All of the collections with backup at the NCGRP are public distributing collections including U.S. NSF-supported genetic stock centers, USDA-ARS collections, and university-supported collections. Facing the retirement of several pioneering researchers, the community discussed the value of preserving personal research collections and agreed that a mechanism to preserve these valuable collections was essential to any future national culture collection system. Additional input from curators of plant and animal collections emphasized that collections of every kind face similar challenges in developing long-range plans for sustainability. C1 [McCluskey, Kevin; Leslie, John] Kansas State Univ, Dept Plant Pathol, Throckmorton Hall, Manhattan, KS 66506 USA. [Alvarez, Anne] Univ Hawaii, Dept Plant & Environm Protect Sci, Manoa, HI USA. [Bennett, Rick] Univ Kentucky, Kentucky Agr Expt Stn, Lexington, KY 40506 USA. [Bokati, Deepak] Samuel Roberts Noble Fdn Inc, Ardmore, OK USA. [Boundy-Mills, Kyria] Univ Calif Davis, Phaff Yeast Culture Collect Food Sci & Technol, Davis, CA 95616 USA. [Brown, Daniel] Univ Florida, Dept Infect Dis & Pathol, Gainesville, FL USA. [Bull, Carolee T.; Fenstermacher, Kristi; Geiser, David] Penn State Univ, Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. [Coffey, Michael] Univ Calif Riverside, World Phytophthora Resource, Riverside, CA 92521 USA. [Dreaden, Tyler] US Forest Serv, USDA, Southern Res Stn, Forest Hlth Res & Educ Ctr, Lexington, KY USA. [Duke, Clifford] Ecol Soc Amer, Off Sci Programs, Washington, DC USA. [Dye, Greg; Ehmke, Erin; Zehr, Sarah] Duke Univ, Duke Lemur Ctr, Durham, NC USA. [Eversole, Kellye] Eversole Associates, 5207 Wyoming Rd, Bethesda, MD USA. [Glaeser, Jessie A.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI USA. [Greene, Stephanie] USDA, Natl Lab Genet Resources Preservat, Ft Collins, CO USA. [Gribble, Lisa] Sandia Natl Labs, Int Biol & Chem Threat Reduct, POB 5800, Albuquerque, NM 87185 USA. [Griffith, M. Patrick] Montgomery Bot Ctr, Coral Gables, FL USA. [Hanser, Kathryn] N Carolina State Univ, Larry F Grand Mycol Herbarium, Raleigh, NC 27695 USA. [Humber, Richard; Ryan, Matthew] USDA ARS, Collect Entomopathogen Fungal Cultures, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Johnson, Barbara W.] US Ctr Dis Control & Prevent, Div Vector Borne Dis, Ft Collins, CO USA. [Kermode, Anthony] CABI, Bakeham Lane, Egham, Surrey, England. [Krichevsky, Micah] Bion Int, Silver Spring, MD USA. [Laudon, Matt] Univ Minnesota, Dept Plant Biol, Chlamydomonas Resource Ctr, St Paul, MN 55108 USA. [Leach, Jan; Silflow, Carolyn] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. [May, Meghan] Univ New England, Coll Osteopath Med, Dept Biomed Sci, Biddeford, ME USA. [Melcher, Ulrich] Oklahoma State Univ, Dept Biochem & Mol Biol, Stillwater, OK 74078 USA. [Nobles, David] Univ Texas Austin, UTEX Culture Collect Algae, Austin, TX 78712 USA. [Fonseca, Natalia Risso] USDA, Rocky Mt Res Stn, Moscow, ID USA. [Robinson, Sara] Oregon State Univ, Coll Forestry, Corvallis, OR 97331 USA. [Scott, James] Univ Toronto, Occupat & Environm Hlth, Toronto, ON M5S 1A1, Canada. [Vidaver, Anne] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. [Webb, Kimberly M.] USDA ARS, Soil Management & Sugar Beet Res Unit, Ft Collins, CO 80526 USA. [Wertz, John E.] Yale Univ, E Coli Genet Stock Ctr, New Haven, CT USA. [Yentsch, Sara] Natl Ctr Marine Algae & Microbiota, Boothbay Harbor, ME USA. [Fonseca, Natalia Risso] Univ Fed Vicosa, Dept Plant Pathol, Vicosa, MG, Brazil. RP McCluskey, K (reprint author), Kansas State Univ, Dept Plant Pathol, Throckmorton Hall, Manhattan, KS 66506 USA. EM mccluskeyk@ksu.edu RI Scott, James/A-8598-2011 OI Scott, James/0000-0002-5073-0832 FU U.S. National Science Foundation [DBI 1203112] FX We thank M. Smith of APS for her support of this meeting, and G. Holman of the USDA NCGRP for support of the microbial program and the meeting of the NRRL. The USCCN is supported by grant DBI 1203112 from the U.S. National Science Foundation. This is publication number 16-171-J of the Kansas Agricultural Experiment Station. NR 63 TC 1 Z9 1 U1 7 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2016 VL 106 IS 6 BP 532 EP 540 DI 10.1094/PHYTO-02-16-0074-RVW PG 9 WC Plant Sciences SC Plant Sciences GA DN1FP UT WOS:000376812000001 PM 26976729 ER PT J AU Bankhead, SB Thomashow, LS Weller, DM AF Bankhead, Stacey Blouin Thomashow, Linda S. Weller, David M. TI Rhizosphere Competence of Wild-Type and Genetically Engineered Pseudomonas brassicacearum Is Affected by the Crop Species SO PHYTOPATHOLOGY LA English DT Article ID FIELD-GROWN WHEAT; BACTERIAL BIOCONTROL AGENTS; GRAMINIS VAR TRITICI; PHENAZINE-1-CARBOXYLIC ACID; BIOLOGICAL-CONTROL; FLUORESCENT PSEUDOMONAS; PUTIDA WCS358R; CHROMOSOMAL INSERTION; ANTIBIOTIC PRODUCTION; CHLORORAPHIS PCL1391 AB 2,4-Diacetylphloroglucinol (2,4-DAPG)-producing Pseudomonas brassicacearum Q8r1-96 is a highly effective biocontrol agent of take-all disease of wheat. Strain Z30-97, a recombinant derivative of Q8r1-96 containing the phzABCDEFG operon from P. synxantha (formerly P. fluorescens) 2-79 inserted into its chromosome, also produces phenazine-1-carboxylic acid. Rhizosphere population sizes of Q8r1-96, Z30-97, and 2-79, introduced into the soil, were assayed during successive growth cycles of barley, navy bean, or pea under controlled conditions as a measure of the impact of crop species on rhizosphere colonization of each strain. In the barley rhizosphere, Z30-96 colonized less that Q8r1-96 when they were introduced separately, and Q8r1-96 out-competed Z30-96 when the strains were introduced together. In the navy bean rhizosphere, Q8r1-96 colonized better than Z30-97 when the strains were introduced separately. However, both strains had similar population densities when introduced together. Strain Q8r1-96 and Z30-97 colonized the pea rhizosphere equally well when each strain was introduced separately, but Z30-97 out-competed Q8r1-96 when they were introduced together. To our knowledge, this is the first report of a recombinant biocontrol strain of Pseudomonas spp. gaining rhizosphere competitiveness on a crop species. When assessing the potential fate of and risk posed by a recombinant Pseudonzonas sp. in soil, both the identity of the introduced genes and the crop species colonized by the recombinant strain need to be considered. C1 [Bankhead, Stacey Blouin] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Thomashow, Linda S.; Weller, David M.] ARS, USDA, Wheat Hlth Genet & Qual Res Unit, Pullman, WA 99164 USA. RP Weller, DM (reprint author), ARS, USDA, Wheat Hlth Genet & Qual Res Unit, Pullman, WA 99164 USA. EM david.weller@ars.usda.gov FU CSREES Biotechnology Risk Assessment Research Grants Program [2004-33522-15161] FX This research was funded by a grant from the CSREES Biotechnology Risk Assessment Research Grants Program (No. 2004-33522-15161). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 68 TC 0 Z9 0 U1 7 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2016 VL 106 IS 6 BP 554 EP 561 DI 10.1094/PHYTO-09-15-0244-R PG 8 WC Plant Sciences SC Plant Sciences GA DN1FP UT WOS:000376812000003 PM 26926486 ER PT J AU Bakker, MG Acharya, J Moorman, TB Robertson, AE Kaspar, TC AF Bakker, Matthew G. Acharya, Jyotsna Moorman, Thomas B. Robertson, Alison E. Kaspar, Thomas C. TI The Potential for Cereal Rye Cover Crops to Host Corn Seedling Pathogens SO PHYTOPATHOLOGY LA English DT Article ID POLYMERASE-CHAIN-REACTION; RHIZOCTONIA ROOT-ROT; FUSARIUM-GRAMINEARUM; PYTHIUM SPP.; WINTER RYE; SOYBEAN SEED; NITROGEN; GROWTH; GLYPHOSATE; MANAGEMENT AB Cover cropping is a prevalent conservation practice that offers substantial benefits to soil and water quality. However, winter cereal cover crops preceding corn may diminish beneficial rotation effects because two grass species are grown in succession. Here, we show that rye cover crops host pathogens capable of causing corn seedling disease. We isolated Fusarium graminearum, E ox-ysporum, Pythiurn sylvaticum, and P torulosum from roots of rye and demonstrate their pathogenicity on corn seedlings. Over 2 years, we quantified the densities of these organisms in rye roots from several field experiments and at various intervals of time after rye cover crops were terminated. Pathogen load in rye roots differed among fields and among years for particular fields. Each of the four pathogen species increased in density over time on roots of herbicide terminated rye in at least one field site, suggesting the broad potential for rye cover crops to elevate corn seedling pathogen densities. The radicles of corn seedlings planted following a rye cover crop had higher pathogen densities compared with seedlings following a winter fallow. Management practices that limit seedling disease may be required to allow corn yields to respond positively to improvements in soil quality brought about by cover cropping. C1 [Bakker, Matthew G.; Moorman, Thomas B.; Kaspar, Thomas C.] ARS, USDA, Natl Lab Agr & Environm, 1015 N Univ Blvd, Ames, IA 50011 USA. [Acharya, Jyotsna; Robertson, Alison E.] Iowa State Univ, Dept Plant Pathol & Microbiol, 351 Bessey Hall, Ames, IA 50011 USA. RP Bakker, MG (reprint author), ARS, USDA, Natl Lab Agr & Environm, 1015 N Univ Blvd, Ames, IA 50011 USA. EM Matt.Bakker@ars.usda.gov OI Robertson, Alison/0000-0003-4281-6350 NR 71 TC 0 Z9 0 U1 11 U2 14 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2016 VL 106 IS 6 BP 591 EP 601 DI 10.1094/PHYTO-02-09-15-0214-R PG 11 WC Plant Sciences SC Plant Sciences GA DN1FP UT WOS:000376812000007 PM 26926485 ER PT J AU Al-Naamani, N Sagliani, KD Dolnikowski, GG Warburton, RR Toksoz, D Kayyali, U Hill, NS Fanburg, BL Roberts, KE Preston, IR AF Al-Naamani, Nadine Sagliani, Kristen D. Dolnikowski, Gregory G. Warburton, Rod R. Toksoz, Deniz Kayyali, Usamah Hill, Nicholas S. Fanburg, Barry L. Roberts, Kari E. Preston, Ioana R. TI Plasma 12-and 15-hydroxyeicosanoids are predictors of survival in pulmonary arterial hypertension SO PULMONARY CIRCULATION LA English DT Article DE pulmonary arterial hypertension; hypoxia-induced pulmonary hypertension; eicosanoids; prostaglandins; hydroxyeicosanoids; prognosis ID ENDOTHELIUM-DEPENDENT CONTRACTIONS; SMOOTH-MUSCLE-CELLS; DISEASE MANAGEMENT REVEAL; CONTROLLED-TRIAL; HYPOXIA; PROSTACYCLIN; EXPRESSION; ACID; THROMBOXANE-A2; TREPROSTINIL AB This study aimed to characterize alterations in select eicosanoids in experimental and human pulmonary arterial hypertension (PAH) and to assess their potential utility as predictors of outcome. Using liquid chromatography-mass spectrometry, we performed targeted lipidomic analyses of the lungs and right ventricles (RVs) of chronically hypoxic rats and plasma of consecutive PAH patients and healthy controls. In rat lungs, chronic hypoxia was associated with significantly decreased lung prostacyclin (PGI(2))/thromboxane B-2 (TXB2) ratio and elevated lung 8-hydroxyeicosanoid (HETE) acid concentrations. RV eicosanoids did not exhibit any changes with chronic hypoxia. PAH treatment-naive patients had significantly increased plasma concentrations of TXB2 and 5-, 8-, 12-, and 15-HETE. The PGI(2)/TXB2 ratio was lower in PAH patients than in controls, especially in the treatment-naive cohort (median: 2.1, 0.3, and 1.3 in controls, treatment-naive, and treated patients, respectively, P =0.001). Survival was significantly worse in PAH patients with 12-HETEhigh (>= 57 pg/mL) and 15-HETEhigh (>= 256 pg/mL) in unadjusted and adjusted analyses (hazard ratio [HR]: 2.8 [95% confidence interval (CI): 1.1-7.3], P=0.04 and HR: 4.3 [95% CI: 1.6-11.8], P=0.004, respectively; adjustment was performed with the REVEAL [Registry to Evaluate Early and Long-Term PAH Disease Management] risk score). We demonstrate significant alterations in eicosanoid pathways in experimental and human PAH. We found that 12- and 15-HETE were independent predictors of survival in human PAH, even after adjusting for the REVEAL score, suggesting their potential role as novel biomarkers. C1 [Al-Naamani, Nadine; Sagliani, Kristen D.; Warburton, Rod R.; Toksoz, Deniz; Kayyali, Usamah; Hill, Nicholas S.; Fanburg, Barry L.; Roberts, Kari E.; Preston, Ioana R.] Tufts Univ, Tufts Med Ctr, Sch Med, Pulm Crit Care & Sleep Div, Boston, MA 02111 USA. [Al-Naamani, Nadine] Tufts Univ, Sackler Sch Grad Biomed Sci, Boston, MA 02111 USA. [Dolnikowski, Gregory G.] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer US Dept Agr, Mass Spectrometry Lab, Boston, MA 02111 USA. RP Preston, IR (reprint author), Tufts Med Ctr, Pulm Crit Care & Sleep Div, 800 Washington St,Box 257, Boston, MA 02111 USA. EM ipreston@tuftsmedicalcenter.org FU National Center for Advancing Translational Sciences, National Institutes of Health [UL1 TR001064, TL1 TR001062]; National Heart, Lung, and Blood Institute [K08 HL7734] FX NA is supported by the National Center for Advancing Translational Sciences, National Institutes of Health grants UL1 TR001064 and TL1 TR001062. IRP was supported by National Heart, Lung, and Blood Institute grant K08 HL7734. NR 46 TC 0 Z9 0 U1 2 U2 4 PU UNIV CHICAGO PRESS PI CHICAGO PA 1427 E 60TH ST, CHICAGO, IL 60637-2954 USA SN 2045-8932 EI 2045-8940 J9 PULM CIRC JI Pulm. Circ. PD JUN PY 2016 VL 6 IS 2 BP 224 EP 233 DI 10.1086/686311 PG 10 WC Cardiac & Cardiovascular Systems; Respiratory System SC Cardiovascular System & Cardiology; Respiratory System GA DM6FJ UT WOS:000376445700011 PM 27252849 ER PT J AU Makarikov, AA Hoberg, EP AF Makarikov, Arseny A. Hoberg, Eric P. TI Broadening diversity in the Arostrilepis horrida complex: Arostrilepis kontrimavichusi n. sp (Cyclophyllidea: Hymenolepididae) in the western red-backed vole Myodes californicus (Merriam) (Cricetidae: Arvicolinae) from temperate latitudes of the Pacific Northwest, North America SO SYSTEMATIC PARASITOLOGY LA English DT Article ID PALEARCTIC MICROTINE RODENTS; EUCESTODA HYMENOLEPIDIDAE; CESTODA HYMENOLEPIDIDAE; PARASITE DIVERSITY; GEOMYIDAE; DIVERSIFICATION; REDESCRIPTION; THOMOMYS; CLIMATE AB Specimens originally identified provisionally as Hymenolepis horrida (Linstow, 1901) [later Arostrilepis horrida (Linstow, 1901)] in Myodes californicus (Merriam) from near the Pacific coastal zone of southern Oregon are revised. Specimens in western red-backed voles represent an undescribed species of Arostrilepis Mas Coma & Tenora, 1997, contributing to recognition and resolution of a broadening complex encompassing cryptic diversity for these hymenolepidid tapeworms distributed across the Holarctic region. Consistent with recent studies defining diversity in the genus, the form, dimensions, and spination (pattern, shape and size) of the cirrus are diagnostic. Among 12 nominal congeners, specimens of A. kontrimavichusi n. sp. are further distinguished by the relative position and length of the cirrus-sac, arrangement of the testes and relative size of the external seminal vesicle and seminal receptacle. Specimens from Oregon voles represent the fifth endemic hymenolepidid in this genus from the Nearctic. Host range for the North American assemblage of species includes Cricetidae (Arvicolinae and Neotominae), Heteromyidae, Geomyidae, and rarely Sciuridae. C1 [Makarikov, Arseny A.] Russian Acad Sci, Siberian Branch, Inst Systemat & Ecol Anim, Frunze Str 11, Novosibirsk 630091, Russia. [Hoberg, Eric P.] ARS, US Natl Parasite Collect, Anim Parasit Dis Lab, USDA, BARC East 1180,10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Makarikov, AA (reprint author), Russian Acad Sci, Siberian Branch, Inst Systemat & Ecol Anim, Frunze Str 11, Novosibirsk 630091, Russia. EM makarikov@mail.ru RI Makarikov, Arseny/I-5516-2013 OI Makarikov, Arseny/0000-0002-4389-8397 FU Federal Fundamental Scientific Research Program [VI.51.1.7.]; Russian Fund for Fundamental Research [14-04-00871-a]; National Science Foundation (NSF); Division of Biological Infrastructure, Biological Research Collections (BRC) [1057383]; NSF [DEB-0196095, 0415668, 1250810] FX AAM was supported in part by the Federal Fundamental Scientific Research Program for 2013-2020 (VI.51.1.7.) and the Russian Fund for Fundamental Research (Project No. 14-04-00871-a). We acknowledge support of the National Science Foundation (NSF) for development of the RHC as a vital resource for helminthology and biogeography through a grant to J.A. Cook, G. Jarrell, S. Loker and EPH from the Division of Biological Infrastructure, Biological Research Collections (BRC 1057383). Further, this research represents a continuing contribution of the Beringian Coevolution Project and the Integrated Inventories of Biomes of the Arctic through grants from the NSF to JAC, EPH, KEG and E. DeChaine (DEB-0196095, 0415668 and 1250810). NR 32 TC 0 Z9 0 U1 3 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0165-5752 EI 1573-5192 J9 SYST PARASITOL JI Syst. Parasitol. PD JUN PY 2016 VL 93 IS 5 BP 467 EP 477 DI 10.1007/s11230-016-9640-1 PG 11 WC Parasitology SC Parasitology GA DN0PC UT WOS:000376764800004 PM 27221000 ER PT J AU Ghanbarian, B Torres-Verdin, C Skaggs, TH AF Ghanbarian, Behzad Torres-Verdin, Carlos Skaggs, Todd H. TI Quantifying tight-gas sandstone permeability via critical path analysis SO ADVANCES IN WATER RESOURCES LA English DT Article DE Critical path analysis; Electrical conductivity; Intrinsic permeability; Mercury intrusion porosimetry ID UNSATURATED HYDRAULIC CONDUCTIVITY; POROUS-MEDIA; ELECTRICAL-CONDUCTIVITY; TRANSPORT-PROPERTIES; RESIDUAL SATURATION; DISORDERED-SYSTEMS; PERCOLATION THEORY; SIZE DISTRIBUTION; WIDE DISTRIBUTION; PORE NETWORKS AB Rock permeability has been actively investigated over the past several decades by the geosciences community. However, its accurate estimation still presents significant technical challenges, particularly in spatially complex rocks. In this short communication, we apply critical path analysis (CPA) to estimate permeability in porous rocks from measured mercury intrusion porosimetry and electrical conductivity data. Theoretical estimations of various CPA-based models are then compared to experimental measurements using eighteen tight-gas sandstones. Except for two of the samples, we find permeability estimations performed with the Skaggs model (assuming pore diameter independent of its length) more accurate than other models, within a factor of two of the measured permeabilities. We discuss some plausible sources of the uncertainties. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Ghanbarian, Behzad; Torres-Verdin, Carlos] Univ Texas Austin, Dept Petr & Geosyst Engn, Austin, TX 78712 USA. [Skaggs, Todd H.] USDA ARS, US Salin Lab, 4500 Glenwood Dr, Riverside, CA 92501 USA. RP Ghanbarian, B (reprint author), Univ Texas Austin, Dept Petr & Geosyst Engn, Austin, TX 78712 USA. EM ghanbarian@austin.utexas.edu OI Ghanbarian, Behzad/0000-0002-7002-4193 NR 54 TC 0 Z9 0 U1 8 U2 15 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0309-1708 EI 1872-9657 J9 ADV WATER RESOUR JI Adv. Water Resour. PD JUN PY 2016 VL 92 BP 316 EP 322 DI 10.1016/j.advwatres.2016.04.015 PG 7 WC Water Resources SC Water Resources GA DL9EW UT WOS:000375945600025 ER PT J AU Noh, SM Dark, MJ Reif, KE Ueti, MW Kappmeyer, LS Scoles, GA Palmer, GH Brayton, KA AF Noh, Susan M. Dark, Michael J. Reif, Kathryn E. Ueti, Massaro W. Kappmeyer, Lowell S. Scoles, Glen A. Palmer, Guy H. Brayton, Kelly A. TI Superinfection Exclusion of the Ruminant Pathogen Anaplasma marginale in Its Tick Vector Is Dependent on the Time between Exposures to the Strains SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID MAMMALIAN RESERVOIR HOST; ANTIGENIC VARIATION; BORNE TRANSMISSION; HIGH PREVALENCE; CROSS-IMMUNITY; INFECTION; DIVERSITY; MALARIA; MIDGUT AB The remarkable genetic diversity of vector-borne pathogens allows for the establishment of superinfection in the mammalian host. To have a long-term impact on population strain structure, the introduced strains must also be transmitted by a vector population that has been exposed to the existing primary strain. The sequential exposure of the vector to multiple strains frequently prevents establishment of the second strain, a phenomenon termed superinfection exclusion. As a consequence, superinfection exclusion may greatly limit genetic diversity in the host population, which is difficult to reconcile with the high degree of genetic diversity maintained among vector-borne pathogens. Using Anaplasma marginale, a tick-borne bacterial pathogen of ruminants, we hypothesized that superinfection exclusion is temporally dependent and that longer intervals between strain exposures allow successful acquisition and transmission of a superinfecting strain. To test this hypothesis, we sequentially exposed Dermacentor andersoni ticks to two readily tick-transmissible strains of A. marginale. The tick feedings were either immediately sequential or 28 days apart. Ticks were allowed to transmission feed and were individually assessed to determine if they were infected with one or both strains. The second strain was excluded from the tick when the exposure interval was brief but not when it was prolonged. Midguts and salivary glands of individual ticks were superinfected and transmission of both strains occurred only when the exposure interval was prolonged. These findings indicate that superinfection exclusion is temporally dependent, which helps to account for the differences in pathogen strain structure in tropical compared to temperate regions. C1 [Noh, Susan M.; Ueti, Massaro W.; Kappmeyer, Lowell S.; Scoles, Glen A.] ARS, Anim Dis Res Unit, USDA, Pullman, WA USA. [Noh, Susan M.; Dark, Michael J.; Reif, Kathryn E.; Palmer, Guy H.; Brayton, Kelly A.] Washington State Univ, Dept Vet Microbiol & Pathol, Program Vector Borne Dis, Pullman, WA 99164 USA. [Noh, Susan M.; Palmer, Guy H.; Brayton, Kelly A.] Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. [Dark, Michael J.] Univ Florida, Coll Vet Med, Dept Infect Dis & Pathol, Gainesville, FL USA. RP Noh, SM (reprint author), ARS, Anim Dis Res Unit, USDA, Pullman, WA USA.; Noh, SM (reprint author), Washington State Univ, Dept Vet Microbiol & Pathol, Program Vector Borne Dis, Pullman, WA 99164 USA.; Noh, SM (reprint author), Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. EM susan.noh@usda.ars.gov OI Dark, Michael/0000-0002-6547-1298 FU United States Department of Agriculture [5348-32000-027-00D, 5348-32000-033-00D]; United States Department of Agriculture-NRICGP [2005-35604-15440]; HHS \ National Institutes of Health (NIH) [K08 AI064162, R37 AI44005] FX This work, including the efforts of Susan M. Noh, Kathryn Elizabeth Reif, Massaro W. Ueti, Lowell Kappmeyer, and Glen A. Scoles, was funded by United States Department of Agriculture (5348-32000-027-00D and 5348-32000-033-00D). This work, including the efforts of Kelly A. Brayton, was funded by United States Department of Agriculture-NRICGP (2005-35604-15440). This work, including the efforts of Michael J. Dark, was funded by HHS vertical bar National Institutes of Health (NIH) (K08 AI064162). This work, including the efforts of Guy H. Palmer, was funded by HHS vertical bar National Institutes of Health (NIH) (R37 AI44005). NR 35 TC 0 Z9 0 U1 1 U2 3 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JUN PY 2016 VL 82 IS 11 BP 3217 EP 3224 DI 10.1128/AEM.00190-16 PG 8 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DM2EL UT WOS:000376159400007 PM 26994084 ER PT J AU Van Vliet, D Wiens, GD Loch, TP Nicolas, P Faisal, M AF Van Vliet, Danielle Wiens, Gregory D. Loch, Thomas P. Nicolas, Pierre Faisal, Mohamed TI Genetic Diversity of Flavobacterium psychrophilum Isolates from Three Oncorhynchus spp. in the United States, as Revealed by Multilocus Sequence Typing SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID BACTERIAL COLDWATER DISEASE; COLD-WATER DISEASE; 16S RIBOSOMAL-RNA; CYTOPHAGA-PSYCHROPHILA; POPULATION-STRUCTURE; VERTICAL TRANSMISSION; INTRAOVUM INFECTION; RAINBOW-TROUT; SALMONID EGGS; FISH FARMS AB The use of a multilocus sequence typing (MLST) technique has identified the intraspecific genetic diversity of U.S. Flavobacterium psychrophilum, an important pathogen of salmonids worldwide. Prior to this analysis, little U.S.F. psychrophilum genetic information was known; this is of importance when considering targeted control strategies, including vaccine development. Herein, MLST was used to investigate the genetic diversity of 96 F. psychrophilum isolates recovered from rainbow trout (Oncorhynchus mykiss), coho salmon (Oncorhynchus kisutch), and Chinook salmon (Oncorhynchus tshawytscha) that originated from nine U.S. states. The isolates fell into 34 distinct sequence types (STs) that clustered in 5 clonal complexes (CCs) (n = 63) or were singletons (n = 33). The distribution of STs varied spatially, by host species, and in association with mortality events. Several STs (i.e., ST9, ST10, ST30, and ST78) were found in multiple states, whereas the remaining STs were localized to single states. With the exception of ST256, which was recovered from rainbow trout and Chinook salmon, all STs were found to infect a single host species. Isolates that were collected during bacterial cold water disease outbreaks most frequently belonged to CC-ST10 (e.g., ST10 and ST78). Collectively, the results of this study clearly demonstrate the genetic diversity of F. psychrophilum within the United States and identify STs of clinical significance. Although the majority of STs described herein were novel, some (e.g., ST9, ST10, ST13, ST30, and ST31) were previously recovered on other continents, which demonstrates the transcontinental distribution of F. psychrophilum genotypes. C1 [Van Vliet, Danielle; Faisal, Mohamed] Michigan State Univ, Dept Fisheries & Wildlife, Coll Agr & Nat Resources, E Lansing, MI 48824 USA. [Wiens, Gregory D.] ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, Kearneysville, WV USA. [Loch, Thomas P.; Faisal, Mohamed] Michigan State Univ, Coll Vet Med, Dept Pathobiol & Diagnost Invest, E Lansing, MI 48824 USA. [Nicolas, Pierre] Univ Paris Saclay, MaIAGE, INRA, Jouy En Josas, France. RP Faisal, M (reprint author), Michigan State Univ, Dept Fisheries & Wildlife, Coll Agr & Nat Resources, E Lansing, MI 48824 USA.; Faisal, M (reprint author), Michigan State Univ, Coll Vet Med, Dept Pathobiol & Diagnost Invest, E Lansing, MI 48824 USA. EM faisal@cvm.msu.edu FU Michigan Department of Natural Resources [751P4300177]; Great Lakes Fishery Trust (GLFT) [2010.1147] FX This work, including the efforts of Danielle Van Vliet, Thomas P. Loch, and Mohamed Faisal, was funded by Michigan Department of Natural Resources (751P4300177). This work, including the efforts of Danielle Van Vliet, Thomas P. Loch, and Mohamed Faisal, was funded by Great Lakes Fishery Trust (GLFT) (2010.1147). NR 46 TC 0 Z9 0 U1 6 U2 14 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JUN PY 2016 VL 82 IS 11 BP 3246 EP 3255 DI 10.1128/AEM.00411-16 PG 10 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DM2EL UT WOS:000376159400010 PM 27016570 ER PT J AU Lohmar, JM Harris-Coward, PY Cary, JW Dhingra, S Calvo, AM AF Lohmar, Jessica M. Harris-Coward, Pamela Y. Cary, Jeffrey W. Dhingra, Sourabh Calvo, Ana M. TI rtfA, a putative RNA-Pol II transcription elongation factor gene, is necessary for normal morphological and chemical development in Aspergillus flavus SO APPLIED MICROBIOLOGY AND BIOTECHNOLOGY LA English DT Article DE rtfA; Aflatoxin; Conidiation; Sclerotium; Aspergillus flavus; Secondary metabolism ID MATING-TYPE GENES; SECONDARY METABOLISM; AFLATOXIN BIOSYNTHESIS; FUNGAL DEVELOPMENT; SCLEROTIAL PRODUCTION; PAF1 COMPLEX; FUSARIUM-VERTICILLIOIDES; SACCHAROMYCES-CEREVISIAE; SEXUAL REPRODUCTION; MOLECULAR-BIOLOGY AB The filamentous fungus Aspergillus flavus is an agriculturally important opportunistic plant pathogen that produces potent carcinogenic compounds called aflatoxins. We identified the A. flavus rtfA gene, the ortholog of rtf1 in Saccharomyces cerevisiae and rtfA in Aspergillus nidulans. Interestingly, rtfA has multiple cellular roles in this mycotoxin-producing fungus. In this study, we show that rtfA regulates conidiation. The rtfA deletion mutant presented smaller conidiophores with significantly reduced conidial production compared to the wild-type strain. The absence of rtfA also resulted in a significant decrease or lack of sclerotial production under conditions that allowed abundant production of these resistance structures in the wild type. Importantly, the deletion of rtfA notably reduced the production of aflatoxin B-1, indicating that rtfA is a regulator of mycotoxin biosynthesis in A. flavus. In addition, the deletion rtfA also altered the production of several unknown secondary metabolites indicating a broader regulatory scope. Furthermore, our study revealed that rtfA controls the expression of the global regulators veA and laeA, which further influence morphogenesis and secondary metabolism in A. flavus. C1 [Lohmar, Jessica M.; Dhingra, Sourabh; Calvo, Ana M.] No Illinois Univ, Dept Biol Sci, 155 Castle Dr, De Kalb, IL 60115 USA. [Harris-Coward, Pamela Y.; Cary, Jeffrey W.] USDA ARS, Food & Feed Safety Res Unit, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Calvo, AM (reprint author), No Illinois Univ, Dept Biol Sci, 155 Castle Dr, De Kalb, IL 60115 USA. EM amcalvo@niu.edu FU USDA [58-6435-4-015] FX This study was funded by USDA grant 58-6435-4-015. NR 68 TC 1 Z9 1 U1 6 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0175-7598 EI 1432-0614 J9 APPL MICROBIOL BIOT JI Appl. Microbiol. Biotechnol. PD JUN PY 2016 VL 100 IS 11 BP 5029 EP 5041 DI 10.1007/s00253-016-7418-7 PG 13 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DM3OG UT WOS:000376255200026 PM 27020290 ER PT J AU Lohmar, JM Harris-Coward, PY Cary, JW Dhingra, S Calvo, AM AF Lohmar, Jessica M. Harris-Coward, Pamela Y. Cary, Jeffrey W. Dhingra, Sourabh Calvo, Ana M. TI rtfA, a putative RNA-Pol II transcription elongation factor gene, is necessary for normal morphological and chemical development in Aspergillus flavus (vol 100, pg 5029, 2016) SO APPLIED MICROBIOLOGY AND BIOTECHNOLOGY LA English DT Correction C1 [Lohmar, Jessica M.; Dhingra, Sourabh; Calvo, Ana M.] No Illinois Univ, Dept Biol Sci, 155 Castle Dr, De Kalb, IL 60115 USA. [Harris-Coward, Pamela Y.; Cary, Jeffrey W.] USDA ARS, Food & Feed Safety Res Unit, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Calvo, AM (reprint author), No Illinois Univ, Dept Biol Sci, 155 Castle Dr, De Kalb, IL 60115 USA. EM amcalvo@niu.edu NR 1 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0175-7598 EI 1432-0614 J9 APPL MICROBIOL BIOT JI Appl. Microbiol. Biotechnol. PD JUN PY 2016 VL 100 IS 11 BP 5043 EP 5043 DI 10.1007/s00253-016-7544-2 PG 1 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DM3OG UT WOS:000376255200027 ER PT J AU Steiner, JJ Buford, MA AF Steiner, J. J. Buford, M. A. TI The Origin of the USDA Regional Biomass Research Centers SO BIOENERGY RESEARCH LA English DT Article DE Biofuels; Sustainability; Policy; Rural development; Growing America's Fuel AB The U.S. Department of Agriculture (USDA) Regional Biomass Research Centers (RBRC) were created to contribute to the planning, research, and development of entire long-term sustainable biofuel production supply chains based on agricultural and forest-based feedstocks. The intent of the centers is to provide a catalyst that links feedstock genetic development, sustainable production and management, logistics, conversion, co-product production, distribution, and market demand suited to the available economic, social, and natural resources within different regions. The centers provide a coordinated, region-based research focus designed with relatively short-term deliverables to help accelerate the commercial production of biomass and other biofuel feedstocks. The centers provide a leadership structure for coordinating biomass research across the country, providing a national perspective that complements other USDA agency efforts designed to help US rural communities participate in the emerging biofuels and biobased products economy. Through coordination with the RBRC, USDA research and service agency programs and resources have been leveraged with the U.S. Department of Energy (DOE) and other federal department, university, and private industry efforts to help accelerate commercial advancement of advanced biofuel production to promote rural economic opportunities and achieve transportation biofuel policy goals. C1 [Steiner, J. J.] NIFA, Natl Program Leader Agron, Inst Food Prod & Sustainabil, USDA,Waterfront Ctr, 800 9th St,SW, Washington, DC 20026 USA. [Buford, M. A.] US Forest Serv, Forest Serv Res & Dev, USDA, Washington, DC 20250 USA. RP Steiner, JJ (reprint author), NIFA, Natl Program Leader Agron, Inst Food Prod & Sustainabil, USDA,Waterfront Ctr, 800 9th St,SW, Washington, DC 20026 USA. EM jeffrey.steiner@nifa.usda.gov FU Agricultural Research Service of the U.S. Department of Agriculture; Forest Service Research of the U.S. Department of Agriculture; USDA National Institute of Food and Agriculture; U.S. Department of Energy Biomass Technology Office; DOE Office of Science FX The research referenced was funded in part by the Agricultural Research Service and Forest Service Research that are intramural research agencies of the U.S. Department of Agriculture. Funding was also provided by the USDA National Institute of Food and Agriculture, and U.S. Department of Energy Biomass Technology Office and DOE Office of Science. The authors thank the guest editors of this issue for their help in arranging the wide spectrum of articles that are presented. Our gratitude to: Ronald Zalesny, USFS, Rhinelander, WI; Judy Libra, ATB, Potsdam, Germany; Douglas Karlen, USDA-ARS, Ames, IA; William Anderson, USDA-ARS, Tifton, GA; Tim Strickland, USDA-ARS, Tifton, GA; Michael Casler, USDA-ARS, Madison, WI; Kevin Smith, University of Melbourne, Australia; Sudhagar Mani, University of Georgia, Athens, GA; and Marilyn Buford, USFS, Washington, DC. Finally, special recognition is given to Dr. Maura O'Neill, former Chief of Staff to Undersecretary Rajiv Shah of the USDA Research, Education, and Economics mission area, who initially conceived the concept of the regional biomass research centers as a vehicle for advancing the development of advanced biofuels needed to help achieve the nation's needs for renewable fuels and other biobased products. NR 0 TC 1 Z9 1 U1 8 U2 10 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2016 VL 9 IS 2 BP 379 EP 383 DI 10.1007/s12155-016-9736-0 PG 5 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA DM3YM UT WOS:000376282600001 ER PT J AU Mitchell, RB Schmer, MR Anderson, WF Jin, V Balkcom, KS Kiniry, J Coffin, A White, P AF Mitchell, R. B. Schmer, M. R. Anderson, W. F. Jin, V. Balkcom, K. S. Kiniry, J. Coffin, A. White, P. TI Dedicated Energy Crops and Crop Residues for Bioenergy Feedstocks in the Central and Eastern USA SO BIOENERGY RESEARCH LA English DT Article DE Big bluestem; Bioenergy; C-4 grasses; Corn stover; Energycane; Miscanthus; Napier grass; Sorghum; Switchgrass ID NORTHERN GREAT-PLAINS; SOIL ORGANIC-CARBON; GREENHOUSE-GAS EMISSIONS; CORN STOVER HARVEST; BIOMASS PRODUCTION; PANICUM-VIRGATUM; COVER CROP; VERTICAL-DISTRIBUTION; CELLULOSIC ETHANOL; PERENNIAL GRASSES AB Dedicated energy crops and crop residues will meet herbaceous feedstock demands for the new bioeconomy in the Central and Eastern USA. Perennial warm-season grasses and corn stover are well-suited to the eastern half of the USA and provide opportunities for expanding agricultural operations in the region. A suite of warm-season grasses and associated management practices have been developed by researchers from the Agricultural Research Service of the US Department of Agriculture (USDA) and collaborators associated with USDA Regional Biomass Research Centers. Second generation biofuel feedstocks provide an opportunity to increase the production of transportation fuels from recently fixed plant carbon rather than from fossil fuels. Although there is no "one-size-fits-all" bioenergy feedstock, crop residues like corn (Zea mays L.) stover are the most readily available bioenergy feedstocks. However, on marginally productive cropland, perennial grasses provide a feedstock supply while enhancing ecosystem services. Twenty-five years of research has demonstrated that perennial grasses like switchgrass (Panicum virgatum L.) are profitable and environmentally sustainable on marginally productive cropland in the western Corn Belt and Southeastern USA. C1 [Mitchell, R. B.] USDA ARS, Grain Forage & Bioenergy Res Unit, UNL, 251 Filley Hall,East Campus, Lincoln, NE 68583 USA. [Schmer, M. R.; Jin, V.] USDA ARS, Agroecosyst Management Res Unit, UNL, 251 Filley Hall,East Campus, Lincoln, NE 68583 USA. [Anderson, W. F.] USDA ARS, Crop Genet & Breeding Res Unit, 2360 Rainwater Rd, Tifton, GA 31794 USA. [Balkcom, K. S.] USDA ARS, Natl Soil Dynam Lab, 411 South Donahue Dr, Auburn, AL 36832 USA. [Kiniry, J.] USDA ARS, Grassland Soil & Water Res Lab, 808 East Blackland Rd, Temple, TX 76502 USA. [Coffin, A.] USDA ARS, Southeast Watershed Res Unit, 2316 Rainwater Rd, Tifton, GA 31793 USA. [White, P.] USDA ARS, Sugarcane Res Unit, 5883 USDA Rd, Houma, LA 70360 USA. RP Mitchell, RB (reprint author), USDA ARS, Grain Forage & Bioenergy Res Unit, UNL, 251 Filley Hall,East Campus, Lincoln, NE 68583 USA. EM rob.mitchell@ars.usda.gov OI Coffin, Alisa/0000-0003-1608-1776 FU Agriculture and Food Research Initiative Competitive Grant from USDA National Institute of Food and Agriculture [2011-68005-30411] FX The US Department of Agriculture (USDA) prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable, sex, marital status, familial status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal, or because all or part of an individual's income is derived from any public assistance program. Mention of trade names or commercial products in this publication does not imply recommendation or endorsement by the USDA. This research was supported by appropriated funds to USDA-ARS, with assistance from an Agriculture and Food Research Initiative Competitive Grant no. 2011-68005-30411 from the USDA National Institute of Food and Agriculture. NR 126 TC 0 Z9 0 U1 11 U2 35 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2016 VL 9 IS 2 BP 384 EP 398 DI 10.1007/s12155-016-9734-2 PG 15 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA DM3YM UT WOS:000376282600002 ER PT J AU Anderson, WF Sarath, G Edme, S Casler, MD Mitchell, RB Tobias, CM Hale, AL Sattler, SE Knoll, JE AF Anderson, W. F. Sarath, G. Edme, S. Casler, M. D. Mitchell, R. B. Tobias, C. M. Hale, A. L. Sattler, S. E. Knoll, J. E. TI Dedicated Herbaceous Biomass Feedstock Genetics and Development SO BIOENERGY RESEARCH LA English DT Article DE Warm-season grasses; Switchgrass; Bioenergy; Energycane; Napiergrass; Sorghum ID PANICUM-VIRGATUM L.; ALCOHOL-DEHYDROGENASE GENE; ACID O-METHYLTRANSFERASE; EXPRESSED SEQUENCE TAGS; CELL-WALL COMPOSITION; BROWN-MIDRIB TRAIT; SWEET SORGHUM; LIGNIN BIOSYNTHESIS; DIVERSITY ANALYSIS; FAMILY SELECTION AB Biofuels and bio-based products can be produced from a wide variety of herbaceous feedstocks. To supply enough biomass to meet the needs of a new bio-based economy, a suite of dedicated biomass species must be developed to accommodate a range of growing environments throughout the USA. Researchers from the US Department of Agriculture's Agricultural Research Service (USDA-ARS) and collaborators associated with the USDA Regional Biomass Research Centers have made major progress in understanding the genetics of switchgrass, sorghum, and other grass species and have begun to use this knowledge to develop new cultivars with high yields and appropriate traits for efficient conversion to bio-based products. Plant geneticists and breeders have discovered genes that reduce recalcitrance for biochemical conversion to ethanol and drop-in fuels. Progress has also been made in finding genes that improve production under biotic and abiotic stress from diseases, pests, and climatic variations. C1 [Anderson, W. F.; Knoll, J. E.] USDA ARS, Crop Genet & Breeding Res Unit, 2360 Rainwater Rd, Tifton, GA 31794 USA. [Sarath, G.; Edme, S.; Mitchell, R. B.; Sattler, S. E.] USDA ARS Grain, Grain Forage & Bioenergy Res, UNL, 251 Filley Hall,East Campus, Lincoln, NE 68553 USA. [Casler, M. D.] USDA ARS, US Dairy Forage Res Ctr, 1925 Linden Dr W, Madison, WI 53706 USA. [Tobias, C. M.] USDA ARS, Crop Improvement & Genet Res, WRRC, 800 Buchanan St, Albany, CA 94710 USA. [Hale, A. L.] USDA ARS, Sugarcane Res Unit, 5883 USDA Rd, Houma, LA 70360 USA. RP Anderson, WF (reprint author), USDA ARS, Crop Genet & Breeding Res Unit, 2360 Rainwater Rd, Tifton, GA 31794 USA. EM bill.anderson@ars.usda.gov NR 132 TC 1 Z9 1 U1 3 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2016 VL 9 IS 2 BP 399 EP 411 DI 10.1007/s12155-015-9709-8 PG 13 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA DM3YM UT WOS:000376282600003 ER PT J AU Long, DS Young, FL Schillinger, WF Reardon, CL Williams, JD Allen, BL Pan, WL Wysocki, DJ AF Long, D. S. Young, F. L. Schillinger, W. F. Reardon, C. L. Williams, J. D. Allen, B. L. Pan, W. L. Wysocki, D. J. TI Development of Dryland Oilseed Production Systems in Northwestern Region of the USA SO BIOENERGY RESEARCH LA English DT Article DE Biomass; Oilseed crops; Stand establishment; Agronomic limitations; Recommendations ID WHEAT TRITICUM-AESTIVUM; THISTLE SALSOLA-IBERICA; NORTHERN GREAT-PLAINS; INTEGRATED WEED MANAGEMENT; CANOLA BRASSICA-NAPUS; WATER-USE EFFICIENCY; GOATGRASS AEGILOPS-CYLINDRICA; SULFUR FERTILIZER MANAGEMENT; DIFFERENT PLANT-POPULATIONS; SEMIARID CANADIAN PRAIRIE AB This report addresses the development of dryland oilseed crops to provide feedstock for production of biofuels in semi-arid portions of the northwestern USA. Bioenergy feedstocks derived from Brassica oilseed crops have been considered for production of hydrotreated renewable jet fuel, but crop growth and yields in the northwestern region are limited by a lack of plant available water. Based on a review of the scientific literature, several areas were identified where research could be directed to provide improvements. The current agronomic limitations for oilseed production are mainly due to seedling establishment under extreme heat, dry seedbeds at optimum planting times, survival under extreme cold, and interspecific competition with weeds. To improve emergence and stand establishment, future work should focus on developing soil management and seeding techniques that optimize plant available water, reduce heat stress, and provide a competitive advantage against weeds that are customized for specific crops, soil types, and soil and environmental conditions. Spring and winter cultivars are needed that offer increased seedling vigor, drought resistance, and cold tolerance. C1 [Long, D. S.; Reardon, C. L.; Williams, J. D.] USDA ARS, Soil & Water Conservat Res Unit, 48037 Tubbs Ranch Rd, Adams, OR 97810 USA. [Young, F. L.] Washington State Univ, USDA ARS, Northwest Sustainable Agroecosyst Res Unit, 215 Johnson Hall, Pullman, WA 99164 USA. [Schillinger, W. F.] Washington State Univ Crop & Soil Sci, Dryland Res Stn, POB B, Lind, WA 99341 USA. [Allen, B. L.] USDA ARS, Agr Syst Res Unit, 1500 North Cent Ave, Sidney, MT 59270 USA. [Pan, W. L.] Washington State Univ Crop & Soil Sci, Pullman, WA 99164 USA. [Wysocki, D. J.] Oregon State Univ, Extens Serv, 2411 NW Carden Ave,Umatilla Hall Rm 100, Pendleton, OR 97801 USA. RP Long, DS (reprint author), USDA ARS, Soil & Water Conservat Res Unit, 48037 Tubbs Ranch Rd, Adams, OR 97810 USA. EM dan.long@ars.usda.gov FU United States Department of Agriculture [2012-10008-19727, 2011-68002-30191]; National Institute of Food and Agriculture; Washington State Biofuel Cropping Systems Project [8016] FX This report was supported by Research Grant Awards [2012-10008-19727 and 2011-68002-30191] from the United States Department of Agriculture, National Institute of Food and Agriculture and the Washington State Biofuel Cropping Systems Project [8016]. NR 163 TC 0 Z9 0 U1 9 U2 18 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2016 VL 9 IS 2 BP 412 EP 429 DI 10.1007/s12155-016-9719-1 PG 18 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA DM3YM UT WOS:000376282600004 ER PT J AU Orts, WJ McMahan, CM AF Orts, W. J. McMahan, C. M. TI Biorefinery Developments for Advanced Biofuels from a Sustainable Array of Biomass Feedstocks: Survey of Recent Biomass Conversion Research from Agricultural Research Service SO BIOENERGY RESEARCH LA English DT Article DE Biorefinery; Ethanol; Fermentation; Biofuel; Biomass; Herbaceous crops; Guayule; Rubber; Pyrolysis ID FUEL ETHANOL-PRODUCTION; PARTHENIUM-ARGENTATUM PYROLYSIS; CATABOLITE REPRESSION MUTANTS; SIMULTANEOUS PRODUCT RECOVERY; PRETREATED LODGEPOLE PINE; DILUTE-ACID PRETREATMENT; ESCHERICHIA-COLI STRAIN; GAS REACTIVE PYROLYSIS; ON-FARM PRETREATMENT; GH43 BETA-XYLOSIDASE AB When the USA passed the Renewable Fuel Standards (RFS) of 2007 into law, it mandated that, by the year 2022, 36 billion gallons of biofuels be produced annually in the USA to displace petroleum. This targeted quota, which required that at least half of domestic transportation fuel be "advanced biofuels" either produced from lignocellulosic feedstocks or be a sustainable liquid fuel other than corn ethanol or biodiesel from vegetable oils, will not likely be met due to the difficulty in commercializing alternative biofuels. The number one cost to a biorefinery is the biomass feedstock cost. Thus, it is important that research into biorefinery strategies be closely coupled to advances in crop science that account for crop yield and crop quality. To reach the RFS targets, stepwise progress in biorefinery technology is needed, as the industry moves from corn ethanol toward utilizing a wider array of lignocellulose-based biomass feedstocks. In 2010, the US Department of Agriculture created five Regional Biomass Research Centers to optimize production, collection, and conversion of crops to bioenergy, thus building a network that fosters collaboration among researchers to improve the biorefinery industry. An important component of the five Regional Biomass Research Centers is the four USDA Agricultural Research Service (ARS) regional utilization laboratories located across the country. These USDA ARS labs were originally set up by their commodities, whereby, in broad terms, the Northern Lab, now NCAUR, focused on corn and soy; the Eastern Lab on oils, leather, dairy, and meats; the Southern Lab on cotton, sugars, and fibers; and the Western Lab on other grains, including wheat and specialty crops. Each lab's traditional expertise in these respective core commodity crops has been maintained as biofuel research came to the fore, but with the addition of new crops and biotechnological expertise, these labs often collaborate with each other, as will be revealed below. This review outlines some of the recent advances from the ARS labs in developing new bioprocessing strategies required to develop bioenergy from new crop sources. C1 [Orts, W. J.; McMahan, C. M.] USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 84710 USA. RP Orts, WJ (reprint author), USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 84710 USA. EM bill.orts@ars.usda.gov NR 145 TC 1 Z9 1 U1 11 U2 23 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2016 VL 9 IS 2 BP 430 EP 446 DI 10.1007/s12155-016-9732-4 PG 17 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA DM3YM UT WOS:000376282600005 ER PT J AU Ro, KS AF Ro, Kyoung S. TI Kinetics and Energetics of Producing Animal Manure-Based Biochar SO BIOENERGY RESEARCH LA English DT Article DE Biochar; Animal manure; Pyrolysis; Kinetics; Energetics ID CHICKEN LITTER; POULTRY LITTER; THERMOCHEMICAL CONVERSION; TEMPERATURE PYROLYSIS; MULTI-UTILIZATION; BIOMASS SOURCE; SWINE MANURE; SOIL; ENERGY; GASIFICATION AB Pyrolysis of animal manure produces biochar with multiple beneficial use potentials for improving soil quality and the environment. The kinetics and energetics of pyrolysis in producing manure-based biochar were reviewed and analyzed. Kinetic analysis of pyrolysis showed that the higher the temperature, the shorter the reaction time was needed for thermal decomposition and carbonization of animal manure. This kinetic information can assist in producing biochar with a desired proximate composition. Biochar with lower volatile matter (VM) content can be produced with either higher pyrolysis temperature or longer reaction time. Energetically, pyrolysis of wet manures is not sustainable due to high energy needed for drying moisture. However, co-pyrolysis with other high energy density wastes such as agricultural plastic wastes would produce not only energetically sustainable biochar but surplus energy as well. This could be used for local power generation. C1 [Ro, Kyoung S.] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC USA. RP Ro, KS (reprint author), USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC USA. EM kyoung.ro@ars.usda.gov FU USDA-ARS National Program [214] FX The author would like to acknowledge the technical and editorial support provided by Dr. Judy A Libra of Leibniz Institute for Agricultural Engineering (ATB), Potsdam-Bornim, Germany. This research is part of the USDA-ARS National Program 214 Agricultural and Industrial Byproduct Utilization. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 41 TC 0 Z9 0 U1 10 U2 16 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2016 VL 9 IS 2 BP 447 EP 453 DI 10.1007/s12155-016-9724-4 PG 7 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA DM3YM UT WOS:000376282600006 ER PT J AU Novak, JM Ippolito, JA Lentz, RD Spokas, KA Bolster, CH Sistani, K Trippe, KM Phillips, CL Johnson, MG AF Novak, J. M. Ippolito, J. A. Lentz, R. D. Spokas, K. A. Bolster, C. H. Sistani, K. Trippe, K. M. Phillips, C. L. Johnson, M. G. TI Soil Health, Crop Productivity, Microbial Transport, and Mine Spoil Response to Biochars SO BIOENERGY RESEARCH LA English DT Article DE Biochar; Microbiology; Mine-impacted spoils; Restoration; Soil health ID BLACK CARBON BIOCHAR; HYDRAULIC-PROPERTIES; WATER AVAILABILITY; ESCHERICHIA-COLI; CONTAMINATED SOILS; CALCAREOUS SOIL; SANDY SOIL; PYROLYSIS; REMEDIATION; AMENDMENTS AB Biochars vary widely in pH, surface area, nutrient concentration, porosity, and metal binding capacity due to the assortment of feedstock materials and thermal conversion conditions under which it is formed. The wide variety of chemical and physical characteristics have resulted in biochar being used as an amendment to rebuild soil health, improve crop yields, increase soil water storage, and restore soils/spoils impacted by mining. Meta-analysis of the biochar literature has shown mixed results when using biochar as a soil amendment to improve crop productivity. For example, in one meta-analysis, biochar increased crop yield by approximately 10 %, while in another, approximately 50 % of the studies reported minimal to no crop yield increases. In spite of the mixed crop yield reports, biochars have properties that can improve soil health characteristics, by increasing carbon (C) sequestration and nutrient and water retention. Biochars also have the ability to bind enteric microbes and enhance metal binding in soils impacted by mining. In this review, we present examples of both effective and ineffective uses of biochar to improve soil health for agricultural functions and reclamation of degraded mine spoils. Biochars are expensive to manufacture and cannot be purged from soil after application, so for efficient use, they should be targeted for specific uses in agricultural and environmental sectors. Thus, we introduce the designer biochar concept as an alternate paradigm stating that biochars should be designed with properties that are tailored to specific soil deficiencies or problems. We then demonstrate how careful selection of biochars can increase their effectiveness as a soil amendment. C1 [Novak, J. M.] USDA ARS, Coastal Plain Res Ctr, Florence, SC 29501 USA. [Ippolito, J. A.; Lentz, R. D.] USDA ARS, Northwest Irrigat & Soils Res Lab, Kimberly, ID 83341 USA. [Spokas, K. A.] USDA ARS, Soil & Water Management Res Unit, St Paul, MN 55108 USA. [Bolster, C. H.; Sistani, K.] USDA ARS, Food Anim Environm Syst Res Unit, Bowling Green, KY 42101 USA. [Trippe, K. M.; Phillips, C. L.] USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. [Johnson, M. G.] US EPA, Western Ecol Div, Natl Hlth & Environm Effects Res Lab, Corvallis, OR 97333 USA. RP Novak, JM (reprint author), USDA ARS, Coastal Plain Res Ctr, Florence, SC 29501 USA. EM jeff.novak@ars.usda.gov FU United States Department of Agriculture-Agricultural Research Service [60-6657-1-024]; US EPA [DE-12-92342301-1] FX Sincere gratitude is expressed to the support staff at involved ARS and United States Environmental Protection Agency (US EPA) locations whose hard work made this research article possible. Parts of the information in this article have been funded through an Interagency Agreement between the United States Department of Agriculture-Agricultural Research Service (60-6657-1-024) and the US EPA (DE-12-92342301-1). It has been subject to review by scientists of the United States Department of Agriculture-Agricultural Research Service (USDA-ARS) at multiple locations and by the National Health and Environment Effects Research Laboratory's Western Ecology Division and approved for journal submission. Approval does not signify that the contents reflect the views of the USDA-ARS or the US EPA, nor does mention of trade names or commercial products constitute endorsement or recommendation for their use. NR 84 TC 3 Z9 3 U1 28 U2 50 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2016 VL 9 IS 2 BP 454 EP 464 DI 10.1007/s12155-016-9720-8 PG 11 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA DM3YM UT WOS:000376282600007 ER PT J AU Zalesny, RS Stanturf, JA Gardiner, ES Perdue, JH Young, TM Coyle, DR Headlee, WL Banuelos, GS Hass, A AF Zalesny, Ronald S., Jr. Stanturf, John A. Gardiner, Emile S. Perdue, James H. Young, Timothy M. Coyle, David R. Headlee, William L. Banuelos, Gary S. Hass, Amir TI Ecosystem Services of Woody Crop Production Systems SO BIOENERGY RESEARCH LA English DT Article DE Biomass; Carbon; Eucalyptus; Pinus; Populus; Provisioning services; Regulating services; Salix; Soils; Water ID SOUTHERN UNITED-STATES; DORMANT HARDWOOD CUTTINGS; WATER-USE EFFICIENCY; FREEZE-TOLERANT EUCALYPTUS; X ENVIRONMENT INTERACTIONS; LOBLOLLY-PINE PLANTATIONS; TREATED WASTE-WATER; 31 POPULUS CLONES; BIOMASS PRODUCTION; RESOURCE AVAILABILITY AB Short-rotation woody crops are an integral component of regional and national energy portfolios, as well as providing essential ecosystem services such as biomass supplies, carbon sinks, clean water, and healthy soils. We review recent USDA Forest Service Research and Development efforts from the USDA Biomass Research Centers on the provisioning of these ecosystem services from woody crop production systems. For biomass, we highlight productivity and yield potential, pest susceptibility, and bioenergy siting applications. We describe carbon storage in aboveground woody biomass and studies assessing the provision of clean and plentiful water. Soil protection and wildlife habitat are also mentioned, in the context of converting lands from traditional row-crop agriculture to woody production systems. C1 [Zalesny, Ronald S., Jr.] US Forest Serv, USDA, No Res Stn, Inst Appl Ecosyst Studies, Rhinelander, WI USA. [Stanturf, John A.] US Forest Serv, USDA, Southern Res Stn, Ctr Forest Disturbance Sci, Athens, GA USA. [Gardiner, Emile S.] US Forest Serv, USDA, Southern Res Stn, Ctr Bottomland Hardwoods Res, Stoneville, MS USA. [Perdue, James H.] US Forest Serv, USDA, Southern Res Stn, Forest Prod Ctr, Knoxville, TN USA. [Young, Timothy M.] Univ Tennessee, Forest Prod Ctr, Knoxville, TN USA. [Coyle, David R.] Univ Georgia, DB Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. [Coyle, David R.] Southern Reg Extens Forestry, Athens, GA USA. [Headlee, William L.] Univ Arkansas, Div Agr, Arkansas Forest Resources Ctr, Monticello, AR USA. [Headlee, William L.] Univ Arkansas, Sch Forestry & Nat Resources, Monticello, AR USA. [Banuelos, Gary S.] USDA ARS, Water Management Res Unit, Parlier, CA USA. [Hass, Amir] West Virginia State Univ, Agr & Environm Res Stn, Institue, WV USA. RP Zalesny, RS (reprint author), US Forest Serv, USDA, No Res Stn, Inst Appl Ecosyst Studies, Rhinelander, WI USA. EM rzalesny@fs.fed.us RI Young, Timothy/D-9949-2011 OI Young, Timothy/0000-0001-9564-6506 FU USDA Forest Service; USDA Biomass Research Centers FX The majority of the research described in this paper was supported by the USDA Forest Service as collaborations associated with the USDA Biomass Research Centers. In addition to agency colleagues, we are grateful to the many external partners who made these collective efforts possible and to the countless number of people who helped us with laboratory, greenhouse, and field work. Furthermore, we thank Dr. Marilyn Buford for her USDA Forest Service leadership, Sue Lietz for producing Fig. 1, and Edmund Bauer and Max Piana for reviewing earlier versions of this manuscript. NR 192 TC 2 Z9 2 U1 9 U2 17 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2016 VL 9 IS 2 BP 465 EP 491 DI 10.1007/s12155-016-9737-z PG 27 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA DM3YM UT WOS:000376282600008 ER PT J AU Zalesny, RS Stanturf, JA Gardiner, ES Banuelos, GS Hallett, RA Hass, A Stange, CM Perdue, JH Young, TM Coyle, DR Headlee, WL AF Zalesny, Ronald S., Jr. Stanturf, John A. Gardiner, Emile S. Banuelos, Gary S. Hallett, Richard A. Hass, Amir Stange, Craig M. Perdue, James H. Young, Timothy M. Coyle, David R. Headlee, William L. TI Environmental Technologies of Woody Crop Production Systems SO BIOENERGY RESEARCH LA English DT Article DE Forest restoration; Mine reclamation; Populus; Phytoremediation; Salix; Urban afforestation ID BOTTOMLAND HARDWOOD FORESTS; LANDFILL LEACHATE; HYBRID POPLAR; POPULUS-MAXIMOWICZII; URBAN BROWNFIELD; CLONAL VARIATION; SALT TOLERANCE; UNITED-STATES; RESTORATION; PHYTOREMEDIATION AB Soil erosion, loss of productivity potential, biodiversity loss, water shortage, and soil and water pollution are ongoing processes that decrease or degrade provisioning (e.g., biomass, freshwater) and regulating (e.g., carbon sequestration, soil quality) ecosystem services. Therefore, developing environmental technologies that maximize these services is essential for the continued support of rural and urban populations. Genotype selection is a key component of these technologies, and characteristics of the species used in short rotation woody biomass systems, as well as the silvicultural techniques developed for short rotation woody crops are readily adapted to environmental applications. Here, we describe the development of such woody crop production systems for the advancement of environmental technologies including phytoremediation, urban afforestation, forest restoration, and mine reclamation. The primary goal of these collective efforts is to develop systems and tools that can help to mitigate ecological degradation and thereby sustain healthy ecosystems across the rural to urban continuum. C1 [Zalesny, Ronald S., Jr.] US Forest Serv, USDA, No Res Stn, Inst Appl Ecosyst Studies, Rhinelander, WI USA. [Stanturf, John A.] US Forest Serv, USDA, Southern Res Stn, Ctr Forest Disturbance Sci, Athens, GA USA. [Gardiner, Emile S.] US Forest Serv, USDA, Southern Res Stn, Ctr Bottomland Hardwoods Res, Stoneville, MS USA. [Banuelos, Gary S.] USDA ARS, Water Management Res Unit, Parlier, CA USA. [Hallett, Richard A.] US Forest Serv, USDA, No Res Stn, New York City Urban Field Stn, Bayside, NY USA. [Hass, Amir] West Virginia State Univ, Agr & Environm Res Stn, Institute, WV USA. [Stange, Craig M.] Nat Resources Conservat Serv, USDA, Bismarck Plant Mat Ctr, Bismarck, ND USA. [Perdue, James H.] US Forest Serv, USDA, Southern Res Stn, Forest Prod Ctr, Knoxville, TN USA. [Young, Timothy M.] Univ Tennessee, Forest Prod Ctr, Knoxville, TN USA. [Coyle, David R.] Univ Georgia, DB Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. [Coyle, David R.] Southern Reg Extens Forestry, Athens, GA USA. [Headlee, William L.] Univ Arkansas, Div Agr, Arkansas Forest Resources Ctr, Monticello, AR USA. [Headlee, William L.] Univ Arkansas, Sch Forestry & Nat Resources, Monticello, AR USA. RP Zalesny, RS (reprint author), US Forest Serv, USDA, No Res Stn, Inst Appl Ecosyst Studies, Rhinelander, WI USA. EM rzalesny@fs.fed.us RI Young, Timothy/D-9949-2011 OI Young, Timothy/0000-0001-9564-6506 FU USDA Forest Service; USDA Agricultural Research Service; USDA Biomass Research Centers FX The majority of the research described in this paper was supported by the USDA Forest Service and USDA Agricultural Research Service as collaborations associated with the USDA Biomass Research Centers. In addition to agency colleagues, we are grateful to the many external partners who made these collective efforts possible and to the countless number of people who helped us with laboratory, greenhouse, and field work. Furthermore, we thank Dr. Marilyn Buford for her USDA Forest Service leadership, Sue Lietz for producing Fig. 1, Irvin Arroyo for producing Figs. 2 and 3, and Edmund Bauer and Max Piana for reviewing earlier versions of this manuscript. NR 74 TC 2 Z9 2 U1 8 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2016 VL 9 IS 2 BP 492 EP 506 DI 10.1007/s12155-016-9738-y PG 15 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA DM3YM UT WOS:000376282600009 ER PT J AU Scott, DA Page-Dumroese, DS AF Scott, D. Andrew Page-Dumroese, Deborah S. TI Wood Bioenergy and Soil Productivity Research SO BIOENERGY RESEARCH LA English DT Article DE Woody biomass; Soil productivity; Biochar ID ORGANIC-MATTER REMOVAL; FOREST FLOOR REMOVAL; PINUS-TAEDA L.; WHOLE-TREE; VEGETATION CONTROL; MICROBIAL BIOMASS; BRITISH-COLUMBIA; BACTERIAL DIVERSITY; HARVEST INTENSITY; MOLECULAR CHARACTERIZATION AB Timber harvesting can cause both short- and long-term changes in forest ecosystem functions, and scientists from USDA Forest Service (USDA FS) have been studying these processes for many years. Biomass and bioenergy markets alter the amount, type, and frequency at which material is harvested, which in turn has similar yet specific impacts on sustainable productivity. The nature of some biomass energy operations provides opportunities to ameliorate or amend forest soils to sustain or improve their productive capacity, and USDA FS scientists are leading the research into these applications. Research efforts to sustain productive soils need to be verified at regional, national, and international scope, and USDA FS scientists work to advance methods for soil quality monitoring and to inform international criteria and indicators. Current and future USDA FS research ranges from detailed soil process studies to regionally important applied research and to broad scale indicator monitoring and trend analysis, all of which will enable the USA to lead in the sustainable production of woody biomass for bioenergy. C1 [Scott, D. Andrew] Alabama A&M Univ, US Forest Serv, USDA, Southern Res Stn, 100 Drake Dr,Rm 101,ARC Bldg, Normal, AL 35762 USA. [Page-Dumroese, Deborah S.] US Forest Serv, USDA, Rocky Mt Res Stn, 1221 South Main St, Moscow, ID 83843 USA. RP Scott, DA (reprint author), Alabama A&M Univ, US Forest Serv, USDA, Southern Res Stn, 100 Drake Dr,Rm 101,ARC Bldg, Normal, AL 35762 USA. EM andyscott@fs.fed.us FU Agriculture and Food Research Initiative from USDA National Institute of Food and Agriculture; Biomass Research and Development Initiative from USDA National Institute of Food and Agriculture [2010-05325]; Department of Energy, Biomass and Research and Development Initiative [DE-EE000629] FX The authors benefited greatly from conversations with Marilyn Buford, who also reviewed earlier drafts of this manuscript. Partial funding was provided by The Agriculture and Food Research Initiative, Biomass Research and Development Initiative, Competitive Grant no. 2010-05325 from the USDA National Institute of Food and Agriculture, and by the Department of Energy, Biomass and Research and Development Initiative, Competitive Grant no. DE-EE000629. NR 119 TC 1 Z9 1 U1 8 U2 12 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2016 VL 9 IS 2 BP 507 EP 517 DI 10.1007/s12155-016-9730-6 PG 11 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA DM3YM UT WOS:000376282600010 ER PT J AU Anderson, N Mitchell, D AF Anderson, Nathaniel Mitchell, Dana TI Forest Operations and Woody Biomass Logistics to Improve Efficiency, Value, and Sustainability SO BIOENERGY RESEARCH LA English DT Article DE Biomass; Feedstocks; Harvesting; Logistics; Operations; Processing ID UNITED-STATES; RESIDUES; BIOCHAR; SYSTEMS; CARBON; SLASH; FUEL; USA AB This paper reviews the most recent work conducted by scientists and engineers of the Forest Service of the US Department of Agriculture (USDA) in the areas of forest operations and woody biomass logistics, with an emphasis on feedstock supply for emerging bioenergy, biofuels, and bioproducts applications. This work is presented in the context of previous research in this field by the agency and is measured against the goals and objectives provided by several important national-level initiatives, including the USDA Regional Biomass Research Centers. Research conducted over the past 5 years in cooperation with a diverse group of research partners is organized in four topic sections: innovative practices, innovative machines, sustainability, and integration. A wide range of studies in operations and logistics address advances in harvest and processing technology, transportation systems, scheduling and planning, feedstock quality, biomass conversion processes, and environmental impacts, including greenhouse gas emissions. We also discuss potential future research to address persistent knowledge gaps, especially those in fire and fuel management. Overall, the research reviewed here aligns well with broad national goals of providing the USA with sustainable and efficient forest biomass management and production systems, specifically including: (1) improved harvest, collection, handling, and transportation systems for woody biomass; (2) cost and equipment information and options for field processing biomass to improve efficiency and mitigate impacts; and (3) forest biomass management systems and technologies to offset impacts and enhance environmental outcomes. However, as needs evolve, professionals in this field must strive to adapt research, development, and dissemination to address relevant future challenges and strengthen capabilities to solve critical problems in the forest sector. C1 [Anderson, Nathaniel] US Forest Serv, Rocky Mt Res Stn, 800 East Beckwith Dr, Missoula, MT 59801 USA. [Mitchell, Dana] US Forest Serv, Southern Res Stn, George W Andrews Forestry Sci Lab, 521 Devall Dr, Auburn, AL 36849 USA. RP Anderson, N (reprint author), US Forest Serv, Rocky Mt Res Stn, 800 East Beckwith Dr, Missoula, MT 59801 USA. EM nathanielmanderson@fs.fed.us NR 85 TC 2 Z9 2 U1 7 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2016 VL 9 IS 2 BP 518 EP 533 DI 10.1007/s12155-016-9735-1 PG 16 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA DM3YM UT WOS:000376282600011 ER PT J AU Rudie, AW Houtman, CJ Groom, LH Nicholls, DL Zhu, JY AF Rudie, Alan W. Houtman, Carl J. Groom, Leslie H. Nicholls, David L. Zhu, J. Y. TI A Survey of Bioenergy Research in Forest Service Research and Development SO BIOENERGY RESEARCH LA English DT Article DE Woody biomass; Forest service; Bioenergy; Biorefinery; Prehydrolysis; Gasification; Co-firing ID PRETREATED LODGEPOLE PINE; WOODY BIOMASS; DOWNDRAFT GASIFIER; ETHANOL-PRODUCTION; HIGH-TITER; FLY-ASH; GASIFICATION; SPORL; COAL; LIGNOSULFONATE AB Forest biomass represents 25-30 % of the annual biomass available in the USA for conversion into bio-based fuels, bio-based chemicals, and bioproducts in general. The USDA Forest Service Research and Development (R&D) has been focused on producing products from forest biomass since its inception in 1905, with direct combustion, solid sawn lumber, pulp and paper, ethanol as fuel, and silvichemicals all among the mission areas of product research and development. The renewed national interest in biomass conversion to fuels and chemicals is supportive of the most critical need of USDA Forest Service R&D, uses for small-diameter trees and other forest biomass that needs to be removed in the fuel mitigation-fire suppression and forest restoration work of the USDA Forest Service. This paper will summarize the recent USDA Forest Service research on direct combustion, fuel pellets, and conversion of forest biomass to ethanol, both as stand-alone biorefinery processes and as an addition to the traditional wood pulping process. C1 [Rudie, Alan W.; Houtman, Carl J.; Zhu, J. Y.] US Forest Serv, USDA, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. [Groom, Leslie H.] US Forest Serv, USDA, Alexandria Forest Ctr, Southern Res Stn, 2500 Shreveport Hwy, Pineville, LA 71360 USA. [Nicholls, David L.] US Forest Serv, USDA, Pacific NW Res Stn, 204 Siginaka Way, Sitka, AK 99835 USA. RP Rudie, AW (reprint author), US Forest Serv, USDA, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. EM arudie@fs.fed.us NR 42 TC 1 Z9 1 U1 6 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2016 VL 9 IS 2 BP 534 EP 547 DI 10.1007/s12155-016-9731-5 PG 14 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA DM3YM UT WOS:000376282600012 ER PT J AU Coffin, AW Strickland, TC Anderson, WF Lamb, MC Lowrance, RR Smith, CM AF Coffin, Alisa W. Strickland, Timothy C. Anderson, William F. Lamb, Marshall C. Lowrance, Richard R. Smith, Coby M. TI Potential for Production of Perennial Biofuel Feedstocks in Conservation Buffers on the Coastal Plain of Georgia, USA SO BIOENERGY RESEARCH LA English DT Article DE Biofuel; Bioenergy feedstocks; Landscape analysis; Miscanthus x giganteus; Pennisetum purpureum; Panicum virgatum; Georgia Coastal Plain ID SWITCHGRASS CULTIVARS; BIOENERGY FEEDSTOCK; BIOMASS PRODUCTION; GRASSES; GROWTH AB With global increases in the production of cellulosic biomass for fuel, or "biofuel," concerns over potential negative effects of using land for biofuel production have promoted attention to concepts of agricultural landscape design that sustainably balance tradeoffs between food, fuel, fiber, and conservation. The Energy Independence Security Act (EISA) of 2007 mandates an increase in advanced biofuels to 21 billion gallons in 2022. The southeastern region of the USA has been identified as a contributor to meeting half of this goal. We used a GIS-based approach to estimate the production and N-removal potential of three perennial biofeedstocks planted as conservation buffers (field borders associated with riparian buffers, and grassed waterways) on the Coastal Plain of Georgia, USA. Land cover, hydrology, elevation, and soils data were used to identify locations within agricultural landscapes that are most susceptible to runoff, erosion, and nutrient loss. We estimated potential annual biomass production from these areas to be: 2.5-3.5 Tg for giant miscanthus (Miscanthus x giganteus), 2-8.6 Tg for "Merkeron" napier grass (Pennisetum purpureum), and 1.9-7.5 Tg for "Alamo" switchgrass (Panicum virgatum). When production strategies were taken into consideration, we estimated total biomass yield of perennial grasses for the Georgia Coastal Plain at 2.2-9.4 Tg year(-1). Using published rates of N removal and ethanol conversion, we calculated the amount of potential N removal by these systems as 8100-51,000 Mg year(-1) and ethanol fuel production as 778-3296 Ml year(-1) (206 to 871 million gal. US). C1 [Coffin, Alisa W.; Strickland, Timothy C.; Smith, Coby M.] USDA ARS, SE Watershed Res Lab, 2316 Rainwater Rd, Tifton, GA 31793 USA. [Anderson, William F.] USDA ARS, Crop Genet & Breeding Res, 115 Coastal Way, Tifton, GA 31794 USA. [Lamb, Marshall C.] USDA ARS, Natl Peanut Res Lab, 1011 Forrester Dr,SE, Dawson, GA 39842 USA. [Lowrance, Richard R.] US EPA, Natl Risk Management Res Lab, 919 Kerr Res Rd, Ada, OK 74820 USA. RP Coffin, AW (reprint author), USDA ARS, SE Watershed Res Lab, 2316 Rainwater Rd, Tifton, GA 31793 USA. EM alisa.coffin@ars.usda.gov OI Coffin, Alisa/0000-0003-1608-1776 NR 50 TC 0 Z9 0 U1 4 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2016 VL 9 IS 2 BP 587 EP 600 DI 10.1007/s12155-015-9700-4 PG 14 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA DM3YM UT WOS:000376282600017 ER PT J AU Plaza, NZ Pingali, SV Qian, S Heller, WT Jakes, JE AF Plaza, Nayomi Z. Pingali, Sai Venkatesh Qian, Shuo Heller, William T. Jakes, Joseph E. TI Informing the improvement of forest products durability using small angle neutron scattering SO CELLULOSE LA English DT Article DE Small angle neutron scattering; Cellulose elementary fibrils; Moisture content; Wood ID X-RAY-SCATTERING; FIBER SATURATION POINT; CELLULOSE MICROFIBRILS; CELL-WALLS; SPRUCE WOOD; CARBON; WATER; HEMICELLULOSE; MICRODIFFRACTION; PRETREATMENT AB A better understanding of how wood nanostructure swells with moisture is needed to accelerate the development of forest products with enhanced moisture durability. Despite its suitability to study nanostructures, small angle neutron scattering (SANS) remains an underutilized tool in forest products research. Nanoscale moisture-induced structural changes in intact and partially cut wood cell walls were investigated using SANS and a custom-built relative humidity (RH) chamber. SANS from intact wood sections cut from each primary wood orientation showed that although wood scattered anisotropically across 1.3-600 nm length scales, measurement of elementary fibril spacing and low-q surface scattering were independent of orientation. Water sorption caused spacing between elementary fibrils to increase with RH, and this swelling accounted for over half the transverse swelling in S2 secondary wood cell walls. Elementary fibril spacing in longitudinally cut wood cells, which were designed to mimic cells near wood-adhesive bondlines, was greater than the spacing in intact cells above 90 % RH. This suggested that some cell wall hoop constraint from the S1 and S3 cell wall layers on the S2 layer was released by cutting the cells. Furthermore, increased spacing between elementary fibrils may also create diffusion channels that are hypothesized to be responsible for the onset of fungal decay in wood. Protocols were established to use SANS in future research to study adhesives and protection treatments to improve moisture durability in forest products. C1 [Plaza, Nayomi Z.] Univ Wisconsin, Mat Sci Program, 1509 Univ Ave, Madison, WI 53706 USA. [Plaza, Nayomi Z.; Jakes, Joseph E.] US Forest Serv, Forest Biopolymer Sci & Engn, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. [Pingali, Sai Venkatesh; Qian, Shuo; Heller, William T.] Oak Ridge Natl Lab, Biol & Soft Matter Div, 1 Bethel Valley Rd, Oak Ridge, TN 37831 USA. RP Jakes, JE (reprint author), US Forest Serv, Forest Biopolymer Sci & Engn, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM jjakes@fs.fed.us OI Plaza Rodriguez, Nayomi/0000-0002-5198-4877; Heller, William/0000-0001-6456-2975 FU GERS program at UW-Madison; NSF GFRP; US Forest Service Pathways program; USDA PECASE awards; FHA Cooperative Research Program for Covered Timber Bridges; Scientific User Facilities Division, Office of Basic Energy Sciences, US Department of Energy; Genomic Science Program, Office of Biological and Environmental Research, US Department of Energy [FWP ERKP752]; Office of Biological and Environmental Research [FWP ERKP291]; Office of Basic Energy Sciences, US Department of Energy [DE-AC05-00OR22725] FX NP acknowledges the GERS program at UW-Madison, 2012 NSF GFRP and US Forest Service Pathways program for support. JEJ acknowledge funding from 2011 USDA PECASE awards. JEJ and NP acknowledge support from the FHA Cooperative Research Program for Covered Timber Bridges. The use of ORNL High Flux Isotope Reactor and Spallation Neutron Source was supported by the Scientific User Facilities Division, Office of Basic Energy Sciences, US Department of Energy. SVP acknowledges the Genomic Science Program, Office of Biological and Environmental Research, US Department of Energy (FWP ERKP752). The Center for Structural Molecular Biology operates the Bio-SANS instrument by support from the Office of Biological and Environmental Research (FWP ERKP291), and its facilities are supported by the Office of Basic Energy Sciences, US Department of Energy and managed by UT-Battelle, LLC under contract no. DE-AC05-00OR22725. NR 56 TC 0 Z9 0 U1 3 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD JUN PY 2016 VL 23 IS 3 BP 1593 EP 1607 DI 10.1007/s10570-016-0933-y PG 15 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA DM1EA UT WOS:000376086900008 ER PT J AU Peng, SX Chang, HB Kumar, S Moon, RJ Youngblood, JP AF Peng, Shane X. Chang, Huibin Kumar, Satish Moon, Robert J. Youngblood, Jeffrey P. TI A comparative guide to controlled hydrophobization of cellulose nanocrystals via surface esterification SO CELLULOSE LA English DT Article DE Cellulose nanocrystals; Esterification; Hydrophobicity; Hansen's solubility parameters; Dispersibility ID NATIVE CELLULOSE; MICROCRYSTALLINE CELLULOSE; SUPRAMOLECULAR STRUCTURE; POLYMER NANOCOMPOSITES; RADICAL POLYMERIZATION; ACID-HYDROLYSIS; NANOWHISKERS; PERFORMANCE; SUSPENSIONS; FILMS AB Surface esterification methods of cellulose nanocrystals (CNC) using acid anhydrides, acid chlorides, acid catalyzed carboxylic acids, and 1'1-carbonyldiimidazole (CDI) activated carboxylic acids were evaluated with acetyl-, hexanoyl-, dodecanoyl-, oleoyl-, and methacryloyl-functionalization. Their grafting efficiency was investigated using Fourier-transform infrared spectroscopy and C-13 solid state NMR spectroscopy. Acid anhydride and CDI were found to be the most applicable reagents to graft short and long chain aliphatic carbons, respectively. The preservation of structural morphology and crystallinity of grafted CNCs were confirmed using transmission electron microscopy and X-ray diffraction. The hydrophobicity of grafted CNCs was evaluated by dispersing them in organic solvents with different Hansen's solubility parameters. The dispersibility of grafted CNCs in organic solvents was improved by using never-dried CNCs as source materials and keep CNCs wet in their washing solvents after grafting, thus increasing the solvency range to disperse CNCs. C1 [Peng, Shane X.; Moon, Robert J.; Youngblood, Jeffrey P.] Purdue Univ, Sch Mat Engn, W Lafayette, IN 47907 USA. [Chang, Huibin; Kumar, Satish] Georgia Inst Technol, Sch Mat Sci & Engn, Atlanta, GA 30332 USA. [Chang, Huibin; Kumar, Satish] Georgia Inst Technol, Renewable Bioprod Inst, Atlanta, GA 30332 USA. [Moon, Robert J.] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA. RP Youngblood, JP (reprint author), Purdue Univ, Sch Mat Engn, W Lafayette, IN 47907 USA. EM jpyoungb@purdue.edu RI Kumar, Satish/F-7308-2011; OI Chang, Huibin/0000-0003-2820-7540 FU National Science Foundation IGERT sustainable electronic Grant [1144843-DGE]; Forest Products Laboratory under USDA [11-JV-11111129-118] FX The authors would like to thank John Harwood from Purdue Interdepartmental NMR Facility for obtaining the 13C solid-state NMR spectra. The research was supported by the National Science Foundation IGERT sustainable electronic Grant #1144843-DGE and the Forest Products Laboratory under USDA Grant: 11-JV-11111129-118. NR 75 TC 1 Z9 1 U1 26 U2 55 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD JUN PY 2016 VL 23 IS 3 BP 1825 EP 1846 DI 10.1007/s10570-016-0912-3 PG 22 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA DM1EA UT WOS:000376086900027 ER PT J AU Wang, WX Mozuch, MD Sabo, RC Kersten, P Zhu, JY Jin, YC AF Wang, Wangxia Mozuch, Michael D. Sabo, Ronald C. Kersten, Phil Zhu, J. Y. Jin, Yongcan TI Endoglucanase post-milling treatment for producing cellulose nanofibers from bleached eucalyptus fibers by a supermasscolloider SO CELLULOSE LA English DT Article DE Endoglucanase; Post-treatment; Cellulose nanofibers; Fibril morphology; Disk grinding/fibrillation ID PYROCOCCUS-HORIKOSHII; HYPERTHERMOSTABLE ENDOGLUCANASE; MECHANICAL-PROPERTIES; BINDING; MICROFLUIDIZATION; PULP AB Three recombinant GH5 endoglucanases chosen for their contrasting hydrolytic activities, and a commercial endoglucanase were used to treat cellulose nanofibers (CNFs) after they were milled from bleached eucalyptus pulp with a supermasscolloider. This enzyme "post-treatment" resulted in different properties for the CNFs depending on enzyme treatment. The degree of polymerization, water retention value and enzymatic digestibility were used to estimate the extent of fibrillation. Morphologies of CNFs were observed by SEM and TEM. Electron microscopy images indicated endoglucanase post-treatment could efficiently improve the nanosize uniformity of CNFs from supermasscolloider fibrillation. All GH5 endoglucanase post-treated CNFs had a diameter between 3 and 19 nm with 70-89 % between 5 and 9 nm. The recombinant GH5 with no reported activity on crystalline cellulose was the most aggressive of the GH5s studied in reducing the DP of CNFs. C1 [Wang, Wangxia; Jin, Yongcan] Nanjing Forestry Univ, Jiangsu Prov Key Lab Pulp & Paper Sci & Technol, Nanjing 210037, Jiangsu, Peoples R China. [Wang, Wangxia; Mozuch, Michael D.; Sabo, Ronald C.; Kersten, Phil; Zhu, J. Y.] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA. RP Kersten, P; Zhu, JY (reprint author), US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA. EM pkersten@fs.fed.us; jzhu@fs.fed.us FU USDA Agriculture and Food Research Initiative (AFRI) Competitive Grant [2011-67009-20056]; Chinese Scholarship Council (CSC); National Natural Science Foundation of China [31370571] FX The authors are grateful for the financial supports by a USDA Agriculture and Food Research Initiative (AFRI) Competitive Grant (No. 2011-67009-20056), Chinese Scholarship Council (CSC), National Natural Science Foundation of China (Grant No. 31370571). The funding from these programs made the visiting appointment of Wang at the USDA Forest Products Laboratory (FPL) possible. We also acknowledge Thomas Kuster of the Analytical Chemistry and Microscopy Lab of FPL for SEM imaging and Debra Sherman of DS imaging LLC, West Lafayette, IN, for TEM imaging. NR 26 TC 1 Z9 1 U1 16 U2 17 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD JUN PY 2016 VL 23 IS 3 BP 1859 EP 1870 DI 10.1007/s10570-016-0946-6 PG 12 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA DM1EA UT WOS:000376086900029 ER PT J AU Mi, HY Liu, CH Chang, TH Seo, JH Zhang, HL Cho, S Behdad, N Ma, ZQ Yao, CH Cai, ZY Gong, SQ AF Mi, Hongyi Liu, Chien-Hao Chang, Tzu-Husan Seo, Jung-Hun Zhang, Huilong Cho, Sang June Behdad, Nader Ma, Zhenqiang Yao, Chunhua Cai, Zhiyong Gong, Shaoqin TI Characterizations of biodegradable epoxy-coated cellulose nanofibrils (CNF) thin film for flexible microwave applications SO CELLULOSE LA English DT Article DE Dielectric characterization; Epoxy coated-cellulose nanofibrils (CNF); Biodegradable; Transmission line measurement; Flexible substrate; RF loss ID LIQUID-CRYSTAL POLYMER; LINES; ELECTRONICS; TRANSISTORS; COMPOSITES; SUBSTRATE AB Wood pulp cellulose nanofibrils (CNF) thin film is a novel recyclable and biodegradable material. We investigated the microwave dielectric properties of the epoxy coated-CNF thin film for potential broad applications in flexible high speed electronics. The characterizations of dielectric properties were carried out in a frequency range of 1-10 GHz. The dielectric constant and loss tangent were extracted by using measurements of microstrip transmission lines that were built on the epoxy coated-CNF film and combined with Agilent Advanced Design System simulations. The RF property for the epoxy coated-CNF was compared to that of commercial polyethylene terephthalate film (PET). With the comparable microwave properties of non-biodegradable PET film, our study suggests that the epoxy coated-CNF film is a suitable biodegradable and environment-friendly substrate for flexible microwave and other applications. C1 [Mi, Hongyi; Liu, Chien-Hao; Chang, Tzu-Husan; Seo, Jung-Hun; Zhang, Huilong; Cho, Sang June; Behdad, Nader; Ma, Zhenqiang] Univ Wisconsin, Dept Elect & Comp Engn, 1415 Johnson Dr, Madison, WI 53706 USA. [Yao, Chunhua; Cai, Zhiyong] USDA, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. [Gong, Shaoqin] Univ Wisconsin, Wisconsin Inst Discovery, Dept Biomed Engn, 330 North Orchard St, Madison, WI 53715 USA. RP Ma, ZQ (reprint author), Univ Wisconsin, Dept Elect & Comp Engn, 1415 Johnson Dr, Madison, WI 53706 USA.; Cai, ZY (reprint author), USDA, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. EM mazq@engr.wisc.edu; zcai@fs.fed.us OI Seo, Jung-Hun/0000-0002-5039-2503 FU AFOSR under a PECASE Grant [FA9550-09-1-0482] FX The work was supported by AFOSR under a PECASE Grant FA9550-09-1-0482. The program manager is Dr. Gernot Pomrenke. NR 30 TC 0 Z9 0 U1 10 U2 18 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD JUN PY 2016 VL 23 IS 3 BP 1989 EP 1995 DI 10.1007/s10570-016-0913-2 PG 7 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA DM1EA UT WOS:000376086900039 ER PT J AU Pimont, F Parsons, R Rigolot, E de Coligny, F Dupuy, JL Dreyfus, P Linn, RR AF Pimont, Francois Parsons, Russell Rigolot, Eric de Coligny, Francois Dupuy, Jean-Luc Dreyfus, Philippe Linn, Rodman R. TI Modeling fuels and fire effects in 3D: Model description and applications SO ENVIRONMENTAL MODELLING & SOFTWARE LA English DT Article DE FuelManager; CAPSIS; Fuel treatment; Fire effects; FIRETEC; WFDS ID PINUS-HALEPENSIS MILL.; CROWN FIRE; SURFACE FIRES; BEETLE OUTBREAKS; EXPERIMENT ICFME; TISSUE NECROSIS; BULK-DENSITY; WIND-FLOWS; FOREST; CANOPY AB Scientists and managers critically need ways to assess how fuel treatments alter fire behavior, yet few tools currently exist for this purpose. We present a spatially-explicit-fuel-modeling system, FuelManager, which models fuels, vegetation growth, fire behavior (using a physics-based model, FIRETEC), and fire effects. FuelManager's flexible approach facilitates modeling fuels across a wide range of detail. Large trees or shrubs with specific coordinates are modeled as individual "Plants", while understory plants are modeled as collections of plants called "LayerSets". Both Plants and LayerSets contain various fuel particles (leaves, needles, twigs) with various properties including shape, size and surface area to volume ratio. A wide range of vegetation and treatments can be modeled, analyzed quantitatively and visualized in a 3D viewer. We describe the modeling approach and demonstrate fuel modeling at different levels of detail, fuel treatment and fire effects capabilities. Detailed model equations are provided in the Appendices. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Pimont, Francois; Rigolot, Eric; Dupuy, Jean-Luc] INRA, URFM, Site Agroparc,CS 40 509, F-84914 Avignon 9, France. [Parsons, Russell] US Forest Serv, USDA, Fire Sci Lab, Missoula, MT 59808 USA. [de Coligny, Francois] INRA, AMAP, F-34398 Montpellier, France. [Linn, Rodman R.] Los Alamos Natl Lab, EES, POB 1663, Los Alamos, NM 87544 USA. [Dreyfus, Philippe] ONF, RDI, F-84000 Avignon, France. RP Pimont, F (reprint author), INRA, URFM, Site Agroparc,CS 40 509, F-84914 Avignon 9, France. EM francois.pimont@avignon.inra.fr FU European Commission, FIREPARADOX research program [FP6-018505]; Joint Fire Science Program of the US Department of Agriculture (USDA); US Department of the Interior (USDI) STANDFIRE Project, JFSP [12-1-03-30] FX This work was made possible by funding of the European Commission as part of the FIREPARADOX research program (contract FP6-018505) as well as partial support from the Joint Fire Science Program of the US Department of Agriculture (USDA) and US Department of the Interior (USDI) STANDFIRE Project, JFSP 12-1-03-30. NR 85 TC 3 Z9 3 U1 5 U2 14 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1364-8152 EI 1873-6726 J9 ENVIRON MODELL SOFTW JI Environ. Modell. Softw. PD JUN PY 2016 VL 80 BP 225 EP 244 DI 10.1016/j.envsoft.2016.03.003 PG 20 WC Computer Science, Interdisciplinary Applications; Engineering, Environmental; Environmental Sciences SC Computer Science; Engineering; Environmental Sciences & Ecology GA DM3AD UT WOS:000376218300018 ER PT J AU Harito, JB Campbell, AT Prestrud, KW Dubey, JP Robertson, LJ AF Harito, Jemere Bekele Campbell, Andrew T. Prestrud, Kristin W. Dubey, J. P. Robertson, Lucy J. TI Surface binding properties of aged and fresh (recently excreted) Toxoplasma gondii oocysts SO EXPERIMENTAL PARASITOLOGY LA English DT Article DE Binding; Lectin; Oocyst; Pepsin; Surface; Toxoplasma; Walls; Wheat germ agglutinin ID WATERBORNE TOXOPLASMOSIS; OCULAR TOXOPLASMOSIS; COCCIDIAN OOCYSTS; DRINKING-WATER; OUTBREAK; LECTIN; WALL; INACTIVATION; RESISTANCE; BRAZIL AB The surfaces of aged (10 years) and fresh (recently excreted) oocysts of Toxoplasma gondii were investigated using monoclonal antibody (mAb) and lectin-binding assays. Fresh oocysts bound a wall-specific mAb labelled with fluorescein isothiocyanate while aged oocysts did not. In contrast, the walls of aged oocysts bound a lectin (wheat germ agglutinin, WGA), but not the walls of fresh oocysts. Exposure of oocysts to detergent solutions or trypsin did not affect the binding properties of the walls of the oocysts. However, exposure of fresh oocysts to acidified pepsin enabled labelling of the walls with WGA, presumably due to the relevant moieties on the oocyst walls becoming exposed. WGA binding, but not mAb binding, was partially abrogated with periodate exposure. These findings reveal a significant difference in the binding properties of oocyst walls from "aged" and "fresh" oocysts. The results are of relevance when considering technologies for isolating or detecting 7: gondii oocysts in environmental samples based on oocyst surface properties, as used for other protozoan parasites. Our results suggest the possibility of developing a WGA-based separation procedure for isolating Toxoplasma oocysts from environmental matrices, in which pepsin pre-treatment would be included to ensure that both fresh and aged oocysts were isolated. (C) 2016 Elsevier Inc. All rights reserved. C1 [Harito, Jemere Bekele; Robertson, Lucy J.] Norwegian Univ Life Sci, Parasitol Lab, Sect Microbiol Immunol & Parasitol, Dept Food Safety & Infect Biol,Fac Vet Med & Bios, POB 8146 Dep, N-0033 Oslo, Norway. [Campbell, Andrew T.] Alere AS, Oslo, Norway. [Prestrud, Kristin W.] Norwegian Kennel Club, POB 8146 Dep, N-0033 Oslo, Norway. [Prestrud, Kristin W.] Norwegian Univ Life Sci, Fac Vet Med & Biosci, Dept Compan Anim Clin Sci, POB 8146 Dep, N-0033 Oslo, Norway. [Dubey, J. P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Bldg 1001,BARC East, Beltsville, MD 20705 USA. RP Robertson, LJ (reprint author), Norwegian Univ Life Sci, Parasitol Lab, Sect Microbiol Immunol & Parasitol, Dept Food Safety & Infect Biol,Fac Vet Med & Bios, POB 8146 Dep, N-0033 Oslo, Norway. EM lucy.robertson@nmbu.no NR 48 TC 0 Z9 0 U1 2 U2 2 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0014-4894 EI 1090-2449 J9 EXP PARASITOL JI Exp. Parasitol. PD JUN PY 2016 VL 165 BP 88 EP 94 DI 10.1016/j.exppara.2016.03.022 PG 7 WC Parasitology SC Parasitology GA DM3CM UT WOS:000376224400013 PM 27003461 ER PT J AU Choi, CS Kingsley, DH AF Choi, Changsun Kingsley, David H. TI Temperature-Dependent Persistence of Human Norovirus Within Oysters (Crassostrea virginica) SO FOOD AND ENVIRONMENTAL VIROLOGY LA English DT Article DE Norovirus; Oyster; Hemocytes; Depuration; Temperature ID NORWALK-LIKE VIRUSES; GASTROINTESTINAL CELLS; HEPATITIS-A; CONTAMINATION; DEPURATION; SHELLFISH; PCR; BIOACCUMULATION; OUTBREAK; ANTIGENS AB This study characterizes the persistence of human norovirus in Eastern oysters (Crassostrea virginica) held at different seawater temperatures. Oysters were contaminated with human norovirus GI.1 (Norwalk strain 8FIIa) by exposing them to virus-contaminated water at 15 A degrees C, and subsequently holding them at 7, 15, and 25 A degrees C for up to 6 weeks. Viral RNA was extracted from oyster tissue and hemocytes and quantitated by RT-qPCR. Norovirus was detected in hemocytes and oysters held at 7 and 15 A degrees C for 6 weeks and in hemocytes and oysters held at 25 A degrees C for up to 2 and 4 weeks, respectively. Results confirm that NoV is quite persistent within oysters and demonstrate that cooler water temperatures extend norovirus clearance times. This study suggests a need for substantial relay times to remove norovirus from contaminated shellfish and suggests that regulatory authorities should consider the effects of water temperature after a suspected episodic norovirus-contamination event. C1 [Choi, Changsun] Chung Ang Univ, Sch Food Sci & Technol, Dept Food & Nutr, Coll Biotechnol & Nat Resources, Ansung 456756, South Korea. [Kingsley, David H.] Delaware State Univ, USDA, ARS, Food Safety & Intervent Technol,James WW Baker Ct, Dover, DE 19901 USA. RP Kingsley, DH (reprint author), Delaware State Univ, USDA, ARS, Food Safety & Intervent Technol,James WW Baker Ct, Dover, DE 19901 USA. EM david.kingsley@ars.usda.gov FU US Department of Agriculture; Agricultural Research Service; National Research Foundation of Korea [NRF-2013R1A1A1064168] FX We wish to thank Gary Richards and Shiowshuh Sheen for critical reading of the manuscript. This work was made possible by intramural funding for the US Department of Agriculture, Agricultural Research Service and a grant from the National Research Foundation of Korea (NRF-2013R1A1A1064168). The US Department of Agriculture is an equal opportunity provider and employer. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply a recommendation or endorsement by the US Department of Agriculture. NR 29 TC 3 Z9 3 U1 4 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1867-0334 EI 1867-0342 J9 FOOD ENVIRON VIROL JI Food Environ. Virol. PD JUN PY 2016 VL 8 IS 2 BP 141 EP 147 DI 10.1007/s12560-016-9234-8 PG 7 WC Environmental Sciences; Microbiology; Virology SC Environmental Sciences & Ecology; Microbiology; Virology GA DM0JO UT WOS:000376030500006 PM 26983441 ER PT J AU Tunick, MH Ren, DXX Van Hekken, DL Bonnaillie, L Paul, M Kwoczak, R Tomasula, PM AF Tunick, Michael H. Ren, Daxi X. Van Hekken, Diane L. Bonnaillie, Laetitia Paul, Moushumi Kwoczak, Raymond Tomasula, Peggy M. TI Effect of heat and homogenization on in vitro digestion of milk SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE milk protein; milk lipid; HTST and UHT processing; casein phosphopeptides ID HIGH-TEMPERATURE PASTEURIZATION; INTESTINAL DIGESTION; WHEY PROTEINS; FAT GLOBULES; WHOLE MILK; DIGESTIBILITY; PROTEOLYSIS; BINDING AB Central to commercial fluid milk processing is the use of high temperature, short time (HTST) pasteurization to ensure the safety and quality of milk, and homogenization to prevent creaming of fat-containing milk. Ultra-high-temperature sterilization is also applied to milk and is typically used to extend the shelf life of refrigerated, specialty milk products or to provide shelf-stable milk. The structures of the milk proteins and lipids are affected by processing but little information is available on the effects of the individual processes or sequences of processes on digestibility. In this study, raw whole milk was subjected to homogenization, HTST pasteurization, and homogenization followed by HTST or UHT processing. Raw skim milk was subjected to the same heating regimens. In vitro gastrointestinal digestion using a fasting model was then used to detect the processing-induced changes in the proteins and lipids. Using sodium dodecyl sulfate-PAGE, gastric pepsin digestion of the milk samples showed rapid elimination of the casein and alpha-lactalbumin bands, persistence of the beta-lactoglobulin bands, and appearance of casein and whey peptide bands. The bands for beta-lactoglobulin were eliminated within the first 15 min of intestinal pancreatin digestion. The remaining proteins and peptides of raw, HTST, and UHT skim samples were digested rapidly within the first 15 min of intestinal digestion, but intestinal digestion of raw and HTST pasteurized whole milk showed some persistence of the peptides throughout digestion. The availability of more lipid droplets upon homogenization, with greater surface area available for interaction with the peptides, led to persistence of the smaller peptide bands and thus slower intestinal digestion when followed by HTST pasteurization but not by UHT processing, in which the denatured proteins may be more accessible to the digestive enzymes. Homogenization and heat processing also affected the zeta-potential and free fatty acid release during intestinal digestion. Stearic and oleic acids were broken down faster than other fatty acids due to their positions on the outside of the triglyceride molecule. Five different casein phosphopeptide sequences were observed after gastric digestion, and 31 sequences were found after intestinal digestion, with activities yet to be explored. Processing affects milk structure and thus digestion and is an important factor to consider in design of foods that affect health and nutrition. C1 [Tunick, Michael H.; Ren, Daxi X.; Van Hekken, Diane L.; Bonnaillie, Laetitia; Paul, Moushumi; Kwoczak, Raymond; Tomasula, Peggy M.] ARS, Dairy & Funct Foods Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Ren, Daxi X.] Zhejiang Univ, Inst Dairy Sci, Coll Anim Sci, Hangzhou 310029, Zhejiang, Peoples R China. [Paul, Moushumi] USDA, Off Outreach Divers & Equal Opportun, ARS, 1400 Independence Ave SW, Washington, DC 20250 USA. RP Tomasula, PM (reprint author), ARS, Dairy & Funct Foods Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM Peggy.Tomasula@ars.usda.gov FU Sustainable strategies to lower the environmental and economic impacts of food processing using fluid milk as a template [1935-41000-087] FX We acknowledge the technical contributions by other co-workers at USDA-ARS-ERRC, Wyndmoor, Pennsylvania: Audrey Thomas-Gahring for nitrogen analysis, James J. Shieh for milk composition analyses, Julia Fischer for SDS-PAGE preparation, Brien Sullivan for sample extractions, and Joseph Uknalis for performing confocal microscopy. We also thank Phoebe Qi for useful discussions and Alberto Nunez for isolating and sequencing the casein phosphopeptides. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. This project was funded under Project 1935-41000-087 "Sustainable strategies to lower the environmental and economic impacts of food processing using fluid milk as a template." This project is a part of National Program 306, Quality and Utilization of Agricultural Products. NR 26 TC 2 Z9 2 U1 26 U2 46 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD JUN PY 2016 VL 99 IS 6 BP 4124 EP 4139 DI 10.3168/jds.2015-10474 PG 16 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DL8FP UT WOS:000375876600003 PM 27060826 ER PT J AU Zanton, GI AF Zanton, G. I. TI Analysis of production responses to changing crude protein levels in lactating dairy cow diets when evaluated in continuous or change-over experimental designs SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE crude protein; milk production; experimental design ID RUMEN-PROTECTED METHIONINE; DISTILLERS DRIED GRAINS; AMINO-ACID SUPPLEMENTATION; MILK-PRODUCTION RESPONSES; SOLVENT SOYBEAN-MEAL; ALFALFA-BASED DIETS; NITROGEN-UTILIZATION; UNDEGRADABLE PROTEIN; METABOLIZABLE PROTEIN; NUTRIENT DIGESTIBILITY AB The objective of this study was to evaluate by meta-analysis the effect of experimental design on the production response functions obtained when changing crude protein (CP) levels in lactating dairy cow diets. The final database of studies meeting the selection criteria contained 55 publications with 23 classified as using a continuous (80 diets) and 34 classified as using a change-over (173 diets) experimental design (2 publications reported results from trials using both designs). Mixed model, weighted analysis of covariance was conducted on production measures in response to CP including the continuous covariates year of publication and average days in milk (DIM) and the discrete classification covariate of experimental design. The model was evaluated for curvilinearity in the response to CP, variance heterogeneity, and coincidence in the response between experimental designs, with P < 0.05 indicating significant effects and P < 0.15 indicating trends. On average, cows in experiments using continuous designs had a lower initial DIM, were on treatment longer, and produced a greater yield of milk and milk protein than cows in experiments using change-over designs. Production responses to increasing CP were increased dry matter intake (DMI), milk and component yield, and feed efficiency (milk Mcal/kg of DMI), and decreased milk N: intake N. Response in milk yield and feed efficiency to increased CP interacted with experimental design where continuous experiments had greater milk yield and feed efficiency response at higher levels of CP. Interaction between CP and design effects on yield of milk protein and fat or milk N: intake N did not approach significance. The database is limited by the lower number of continuous studies and the differences in average DIM between designs; nevertheless, it is concluded that DMI, milk protein yield, milk fat yield, and milk N: intake N responses to CP did not depend on experimental design. Response of milk yield and feed efficiency to CP interacted with experimental design; however, prospective research on the influence of experimental design is required to test these results. C1 [Zanton, G. I.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Zanton, GI (reprint author), USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. EM geoffrey.zanton@ars.usda.gov FU USDA, Agricultural Research Service under National Program 101 Food Animal Production FX This research was supported by funding from USDA, Agricultural Research Service under National Program 101 Food Animal Production. NR 85 TC 0 Z9 0 U1 3 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD JUN PY 2016 VL 99 IS 6 BP 4398 EP 4410 DI 10.3168/jds.2015-10438 PG 13 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DL8FP UT WOS:000375876600029 PM 27060818 ER PT J AU Soder, KJ Brito, AF Hafla, AN Rubano, MD AF Soder, K. J. Brito, A. F. Hafla, A. N. Rubano, M. D. TI Effect of starchy or fibrous carbohydrate supplementation of orchardgrass on ruminal fermentation and methane output in continuous culture SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE barley; beet pulp; continuous culture fermentation; grazing ID BACTERIAL PROTEIN-SYNTHESIS; GRAZING DAIRY-COWS; RUMEN FERMENTATION; CONCENTRATE SUPPLEMENTATION; MICROBIAL FERMENTATION; PERENNIAL RYEGRASS; NUTRIENT DIGESTION; DIURNAL-VARIATION; FIBER DIGESTION; MIXED CULTURES AB A 4-unit dual-flow continuous culture fermentor system was used to assess the effects of supplementing orchardgrass (Dactylis glomerata L.) with 2 levels [5 and 10% of total dry matter (DM) fed] of starchy (barley grain, BAR) or fibrous (beet pulp, BP) carbohydrate sources on nutrient digestibility, volatile fatty acid (VFA) production, bacterial protein synthesis, and CH4 output. Treatments were randomly assigned to fermentors in a 4 x 4 Latin square design with a 2 x 2 factorial arrangement using 7 d for microbial adaptation and 3 d for sample collection. Treatments included (1) 57 g of DM orchardgrass + 3 g of DM BAR, (2) 54 g of DM orchardgrass + 6 g of DM BAR, (3) 57 g of DM orchardgrass + 3 g of DM BP, or (4) 54 g of DM orchardgrass + 6 g of DM BP. Feedings occurred at 0900, 1030, 1400, and 1900 h throughout four 10-d periods. Gas samples for CH4 analysis were collected 6 times daily at 0725, 0900, 1000, 1355, 1530, and 1630 h. Fermentor samples for pH, NH3-N, and VFA analysis were taken on d 8, 9, and 10. Samples were also analyzed for DM, organic matter, crude protein, purines, neutral detergent fiber, and acid detergent fiber to determine nutrient digestibilities and estimation of bacterial protein synthesis. Apparent and true DM and organic matter digestibilities were not affected by supplement source. Apparent neutral and acid detergent fiber digestibilities were greater for BAR than BP. Conversely, apparent crude protein digestibility was greater for BP than BAR. Mean and maximum pH tended to be greatest for BAR than BP. Minimum pH was greater at the lower level (5% of diet DM) of supplementation. Barley produced greater concentrations of total VFA and acetate, whereas BP had greater daily outputs of CH4. Significant supplement type x level interactions were found for bacterial N flow and efficiency. Overall, supplementing orchardgrass with BP improved crude protein digestibility, reduced fiber digestibility and total VFA concentration, but increased CH4 output compared with BAR. C1 [Soder, K. J.; Hafla, A. N.; Rubano, M. D.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Brito, A. F.] Univ New Hampshire, Dept Biol Sci, Durham, NH 03824 USA. RP Soder, KJ (reprint author), USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. EM Kathy.Soder@ars.usda.gov FU USDA-National Institute of Food and Agriculture (NIFA, Washington, DC)-Organic Agriculture Research and Extension Initiative (OREI) FX The authors thank J. Everhart, R. Stout, C. Dell (USDA-Agricultural Research Service) and J. Dillon (Penn State) for laboratory expertise and time contributed to conducting this experiment. This project was partially funded by USDA-National Institute of Food and Agriculture (NIFA, Washington, DC)-Organic Agriculture Research and Extension Initiative (OREI). NR 57 TC 1 Z9 1 U1 3 U2 7 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD JUN PY 2016 VL 99 IS 6 BP 4464 EP 4475 DI 10.3168/jds.2015-10471 PG 12 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DL8FP UT WOS:000375876600034 PM 27060824 ER PT J AU Wiggans, GR Cooper, TA VanRaden, PM Van Tassell, CP Bickhart, DM Sonstegard, TS AF Wiggans, G. R. Cooper, T. A. VanRaden, P. M. Van Tassell, C. P. Bickhart, D. M. Sonstegard, T. S. TI Increasing the number of single nucleotide polymorphisms used in genomic evaluation of dairy cattle SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE single nucleotide polymorphism; genomic evaluation; dairy cattle; beadchip; reliability ID PREDICTIONS; RELIABILITY AB GeneSeek (Neogen Corp., Lexington, KY) designed a new version of the GeneSeek Genomic Profiler HD BeadChip for Dairy Cattle, which originally had >77,000 single nucleotide polymorphisms (SNP). A set of >140,000 SNP was selected that included all SNP on the existing GeneSeek chip, all SNP used in US national genomic evaluations, SNP that were possible functional mutations, and other informative SNP. Because SNP with a lower minor allele frequency might track causative variants better, 30,000 more SNP were selected from the Illumina BovineHD Genotyping BeadChip (Illumina Inc., San Diego, CA) by choosing SNP to maximize differences in minor allele frequency between a SNP being considered for the new chip and the 2 SNP that flanked it. Single-gene tests were included if their location was known and bioinformatics indicated relevance for dairy cattle. To determine which SNP from the new chip should be included in genomic evaluations, genotypes available from chips already in use were used to impute and evaluate the SNP set. Effects for 134,511 usable SNP were estimated for all breed-trait combinations; SNP with the largest absolute values for effects were selected (5,000 for Holsteins, 1,000 for Jerseys, and 500 each for Brown Swiss and Ayrshires for each trait). To increase overlap with the 60,671 SNP currently used for genomic evaluation, 12,094 more SNP with the largest effects were added. After removing SNP with many parent-progeny conflicts, 84,937 SNP remained. Three cutoff studies were conducted with 3 SNP sets to determine reliability gain over that for parent average when evaluations based on August 2011 data were used to predict December 2014 performance. Across all traits, mean Holstein reliability gains were 32.5, 33.4, and 32.0 percentage points for 60,671, 84,937, and 134,511 SNP, respectively. After genotypes from the new chip became available, the proposed set was reduced from 84,937 to 77,321 SNP to remove SNP that were not included during manufacture, reduce computing time, and improve imputation performance. The set of 77,321 SNP was evaluated using August 2011 data to predict April 2015 performance. Reliability gain over 60,671 SNP was 1.4 percentage points across traits for Holsteins. Improvement over 84,937 SNP was partially the result of 4 mo of additional data and genotypes from the new chip. Revision of the SNP set used for genomic evaluation is expected to be an ongoing process to increase evaluation accuracy. C1 [Wiggans, G. R.; Cooper, T. A.; VanRaden, P. M.; Van Tassell, C. P.; Bickhart, D. M.; Sonstegard, T. S.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. [Sonstegard, T. S.] Acceligen Recombinet Inc, St Paul, MN 55104 USA. RP Wiggans, GR (reprint author), ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. EM george.wiggans@ars.usda.gov OI Bickhart, Derek/0000-0003-2223-9285 FU USDA Agricultural Research Service [1245-31000-101-00] FX The authors thank the Council on Dairy Cattle Breeding (Bowie, MD) for supplying pedigree, performance, and genotypic data; D. Pomp (University of North Carolina, Chapel Hill) for insight, advice, and leadership on developing version 2 of the GeneSeek Genomic Profiler HD for Dairy Cattle BeadChip; and S. M. Hubbard (Animal Genomics and Improvement Laboratory, Agricultural Research Service, USDA, Beltsville, MD) for technical manuscript review. The project was supported by USDA Agricultural Research Service appropriated project 1245-31000-101-00, "Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information." NR 18 TC 3 Z9 3 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD JUN PY 2016 VL 99 IS 6 BP 4504 EP 4511 DI 10.3168/jds.2015-10456 PG 8 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DL8FP UT WOS:000375876600038 PM 27040793 ER PT J AU Hoernig, KJ Donovan, DM Pithua, P Williams, F Middleton, JR AF Hoernig, K. J. Donovan, D. M. Pithua, P. Williams, F., III Middleton, J. R. TI Evaluation of a lysostaphin-fusion protein as a dry-cow therapy for Staphylococcus aureus mastitis in dairy cattle SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE Staphylococcus aureus mastitis; lysostaphin; dry-cow therapy ID FIELD GEL-ELECTROPHORESIS; SOMATIC-CELL COUNT; INTRAMAMMARY INFECTION; MAMMARY-GLAND; STRAIN; PATTERNS; HEIFERS; HERD AB This study evaluated the efficacy of a recombinant lysostaphin fused to a protein transduction domain (rLYS-PTD) as a dry-cow therapy for the treatment of experimentally induced chronic, subclinical Staphylococcus aureus mastitis. Twenty-two Holstein dairy cows were experimentally infected with Staph. aureus in a single pair of diagonal mammary quarters approximately 45 d before dry off. Staphylococcus aureus-infected mammary quarters of cows were randomly assigned to 1 of 2 treatment groups at dry off: (1) 279 mg of rLYS-PTD in 50 mL of vehicle (n = 11 cows; 22 quarters) or (2) 50 mL of vehicle solution (n = 11 cows; 22 quarters) by intramammary infusion. All cows were followed for 30 d postpartum to determine cure rates using bacteriologic culture, somatic cell counts, and clinical mastitis scores. No cures were recorded in either the treatment or control groups. Milk somatic cell count, bacterial colony counts, and mastitis scores did not significantly differ between treatment groups. In conclusion, rLYS-PTD was not an effective dry-cow therapeutic for chronic, subclinical Staph. aureus mastitis at the tested dose and formulation. C1 [Hoernig, K. J.; Pithua, P.; Middleton, J. R.] Univ Missouri, Dept Vet Med & Surg, Columbia, MO 65211 USA. [Donovan, D. M.] USDA, Beltsville, MD 20705 USA. [Williams, F., III] Univ Missouri, Vet Med Diagnost Lab, Columbia, MO 65211 USA. RP Middleton, JR (reprint author), Univ Missouri, Dept Vet Med & Surg, Columbia, MO 65211 USA. EM middletonjr@missouri.edu FU USDA National Institute of Food and Agriculture (Washington, DC) [2010-65119-20576] FX This work was supported by a grant from the USDA National Institute of Food and Agriculture (Washington, DC; Award No. 2010-65119-20576). The authors thank USDA Agricultural Research Service (Beltsville, MD) staff member Juli Foster-Frey, and University of Missouri staff Marion Butcher (College of Veterinary Medicine), John Denbigh and Eric Adkins (Foremost Dairy Farm), Julie Holle and Pamela Adkins (Department of Veterinary Medicine and Surgery), Kathryn Notch and Anna Delabar (students) for technical assistance. NR 34 TC 0 Z9 0 U1 8 U2 13 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD JUN PY 2016 VL 99 IS 6 BP 4638 EP 4646 DI 10.3168/jds.2015-10783 PG 9 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DL8FP UT WOS:000375876600051 PM 27040789 ER PT J AU Yee, MS Walker, JP Monerris, A Rudiger, C Jackson, TJ AF Yee, Mei Sun Walker, Jeffrey P. Monerris, Alessandra Ruediger, Christoph Jackson, Thomas J. TI On the identification of representative in situ soil moisture monitoring stations for the validation of SMAP soil moisture products in Australia SO JOURNAL OF HYDROLOGY LA English DT Article DE Soil moisture measurement; Representativeness; Soil Moisture Active Passive (SMAP); Satellite validation; Temporal stability; Spatial stability ID REMOTE-SENSING FOOTPRINTS; TEMPORAL STABILITY; FIELD-SCALE; HYDROLOGY EXPERIMENT; SPATIAL-PATTERNS; SEMIARID STEPPE; TIME STABILITY; WATER CONTENT; MICROWAVE; VARIABILITY AB The high spatio-temporal variability of soil moisture complicates the validation of remotely sensed soil moisture products using in situ monitoring stations. Therefore, a standard methodology for selecting the most representative stations for the purpose of validating satellites and land surface models is essential. Based on temporal stability and geostatistical studies using long-term soil moisture records, intensive ground measurements and airborne soil moisture products, this study investigates the representativeness of soil moisture monitoring stations within the Yanco study area for the validation of NASA's Soil Moisture Active Passive (SMAP) products at 3 km for radar, 9 km for radar radiometer and 36 km for radiometer pixels. This resulted in the identification of a number of representative stations according to the different scales. Although the temporal stability method was found to be suitable for identifying representative stations, stations based on the mean relative difference (MRD) were not necessarily the most representative of the areal average. Moreover, those identified from standard deviation of the relative difference (SDRD) may be dry-biased. It was also found that in the presence of heterogeneous land use, stations should be weighted based on proportions of agricultural land. Airborne soil moisture products were also shown to provide useful a priori information for identifying representative locations. Finally, recommendations are made regarding the design of future networks for satellite validation, and specifically the most representative stations for the Yanco area. (C) 2016 Elsevier B.V. All rights reserved. C1 [Yee, Mei Sun; Walker, Jeffrey P.; Monerris, Alessandra; Ruediger, Christoph] Monash Univ, Dept Civil Engn, Clayton, Vic 3168, Australia. [Jackson, Thomas J.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. RP Yee, MS (reprint author), Monash Univ, Dept Civil Engn, Clayton, Vic 3168, Australia. EM mei.yee@monash.edu FU Australian Research Council (ARC); Australian Postgraduate Award (APA) Scholarship FX This study was made possible through the provision of SMAPEx and OzNet data financially supported by grants from the Australian Research Council (ARC). MS Yee acknowledges the support from the Australian Postgraduate Award (APA) Scholarship. We would also like to thank Frank Winston and Rodger Young who provided valuable contributions in the installation and maintenance of the soil moisture stations. Thanks also to all the SMAPEx 1-3 experiment participants. NR 69 TC 3 Z9 3 U1 12 U2 30 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD JUN PY 2016 VL 537 BP 367 EP 381 DI 10.1016/j.jhydrol.2016.03.060 PG 15 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA DM2XU UT WOS:000376212200034 ER PT J AU Chaimanee, V Evans, JD Chen, YP Jackson, C Pettis, JS AF Chaimanee, Veeranan Evans, Jay D. Chen, Yanping Jackson, Caitlin Pettis, Jeffery S. TI Sperm viability and gene expression in honey bee queens (Apis mellifera) following exposure to the neonicotinoid insecticide imidacloprid and the organophosphate acaricide coumaphos SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Honey bee queen; Apis mellifera; Sperm viability; Imidacloprid; Coumaphos; Gene expression ID COLONY LOSSES; ACETYLCHOLINE-RECEPTORS; PESTICIDE EXPOSURE; OXIDATIVE STRESS; HYMENOPTERA; NOSEMA; APIDAE; EVOLUTION; IMMUNITY; PATHOGEN AB Honey bee population declines are of global concern. Numerous factors appear to cause these declines including parasites, pathogens, malnutrition and pesticides. Residues of the organophosphate acaricide coumaphos and the neonicotinoid insecticide imidacloprid, widely used to combat Varroa mites and for crop protection in agriculture, respectively, have been detected in wax, pollen and comb samples. Here, we assess the effects of these compounds at different doses on the viability of sperm stored in the honey bee queens' spermatheca. Our results demonstrate that sub-lethal doses of imidacloprid (0.02 ppm) decreased sperm viability by 50%, 7 days after treatment. Sperm viability was a downward trend (about 33%) in queens treated with high doses of coumaphos (100 ppm), but there was not significant difference. The expression of genes that are involved in development, immune responses and detoxification in honey bee queens and workers exposed to chemicals was measured by qPCR analysis. The data showed that expression levels of specific genes were triggered 1 day after treatment. The expression levels of P450 subfamily genes, CYP306A1, CYP4G11 and CYP6AS14 were decreased in honey bee queens treated with low doses of coumaphos (5 ppm) and imidacloprid (0.02 ppm). Moreover, these two compounds suppressed the expression of genes related to antioxidation, immunity and development in queens at day 1. Up-regulation of antioxidants by these compounds in worker bees was observed at day 1. Coumaphos also caused a repression of CYP306A1 and CYP4G11 in workers. Antioxidants appear to prevent chemical damage to honey bees. We also found that DWV replication increased in workers treated with imidacloprid. This research clearly demonstrates that chemical exposure can affect sperm viability in queen honey bees. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Chaimanee, Veeranan] Maejo Univ, Dept Biotechnol, Phrae Campus, Rong Kwang 54140, Phrae, Thailand. [Evans, Jay D.; Chen, Yanping; Jackson, Caitlin; Pettis, Jeffery S.] ARS, Bee Res Lab, USDA, Beltsville, MD USA. RP Chaimanee, V (reprint author), Maejo Univ, Dept Biotechnol, Phrae Campus, Rong Kwang 54140, Phrae, Thailand. EM chveeranan@gmail.com OI Evans, Jay/0000-0002-0036-4651 FU USDA-ARS Areawide Program FX We thank Nathan Rice and Katie Joselow for their valuable help to conduct the experiments, I. Barton Smith for assistance with the honey bee queens, Dawn Lopez for providing technical and molecular assistance and Michele Hamilton for help with revisions of the manuscript. Funding was provided in part from the USDA-ARS Areawide Program to improve colony health. NR 64 TC 2 Z9 2 U1 48 U2 98 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 EI 1879-1611 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD JUN PY 2016 VL 89 BP 1 EP 8 DI 10.1016/j.jinsphys.2016.03.004 PG 8 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA DM2XL UT WOS:000376211300001 PM 26979384 ER PT J AU Bansal, S Sheley, RL AF Bansal, Sheel Sheley, Roger L. TI Annual grass invasion in sagebrush steppe: the relative importance of climate, soil properties and biotic interactions SO OECOLOGIA LA English DT Article DE Bromus tectorum; Cheatgrass; Climate change; Perennial grasses; Structural equation modeling ID MICROBIAL BIOMASS CARBON; BROMUS-TECTORUM INVASION; EXOTIC PLANT INVASIONS; NATIVE PERENNIAL GRASS; GREAT-BASIN; NUTRIENT AVAILABILITY; BIOLOGICAL INVASIONS; SPECIES ABUNDANCE; NITROGEN; COMMUNITIES AB The invasion by winter-annual grasses (AGs) such as Bromus tectorum into sagebrush steppe throughout the western USA is a classic example of a biological invasion with multiple, interacting climate, soil and biotic factors driving the invasion, although few studies have examined all components together. Across a 6000-km(2) area of the northern Great Basin, we conducted a field assessment of 100 climate, soil, and biotic (functional group abundances, diversity) factors at each of 90 sites that spanned an invasion gradient ranging from 0 to 100 % AG cover. We first determined which biotic and abiotic factors had the strongest correlative relationships with AGs and each resident functional group. We then used regression and structural equation modeling to explore how multiple ecological factors interact to influence AG abundance. Among biotic interactions, we observed negative relationships between AGs and biodiversity, perennial grass cover, resident species richness, biological soil crust cover and shrub density, whereas perennial and annual forb cover, tree cover and soil microbial biomass had no direct linkage to AG. Among abiotic factors, AG cover was strongly related to climate (increasing cover with increasing temperature and aridity), but had weak relationships with soil factors. Our structural equation model showed negative effects of perennial grasses and biodiversity on AG cover while integrating the negative effects of warmer climate and positive influence of belowground processes on resident functional groups. Our findings illustrate the relative importance of biotic interactions and climate on invasive abundance, while soil properties appear to have stronger relationships with resident biota than with invasives. C1 [Bansal, Sheel; Sheley, Roger L.] USDA ARS, Eastern Oregon Agr Res Ctr, 67826-A Hwy 205, Burns, OR 97720 USA. [Bansal, Sheel] US Geol Survey, Northern Prairie Wildlife Res Ctr, 8711 37th St SE, Jamestown, ND 58401 USA. RP Bansal, S (reprint author), USDA ARS, Eastern Oregon Agr Res Ctr, 67826-A Hwy 205, Burns, OR 97720 USA.; Bansal, S (reprint author), US Geol Survey, Northern Prairie Wildlife Res Ctr, 8711 37th St SE, Jamestown, ND 58401 USA. EM sheelbansal9@gmail.com FU USDA-Agricultural Research Service FX We thank the many technicians who assisted us with field data collection and laboratory analyses, especially C. Park, C. Gearhart and B. Bingham. We are also indebted to numerous scientists that helped with the development of various protocols used in this study, with special thanks to Drs. W. Cheng for help with the SIR protocol, M. Reisner, K. Ford and B. Cade who provided statistical advice, and M. Jonsson and two anonymous reviewers for editing. This research was funded through the USDA-Agricultural Research Service Areawide Project for Ecologically-based Invasive Plant Management of Annual Grasses in the Great Basin Ecosystem. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the US Government. NR 89 TC 2 Z9 2 U1 18 U2 42 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 EI 1432-1939 J9 OECOLOGIA JI Oecologia PD JUN PY 2016 VL 181 IS 2 BP 543 EP 557 DI 10.1007/s00442-016-3583-8 PG 15 WC Ecology SC Environmental Sciences & Ecology GA DM4DO UT WOS:000376296000020 PM 26920900 ER PT J AU Lin, DJ Hua, YN Zhang, QZ Xu, DH Fu, YW Liu, YM Zhou, SY AF Lin, De-Jie Hua, Ya-Nan Zhang, Qi-Zhong Xu, De-Hai Fu, Yao-Wu Liu, Yan-Meng Zhou, Sheng-Yu TI Evaluation of medicated feeds with antiparasitical and immune-enhanced Chinese herbal medicines against Ichthyophthirius multifiliis in grass carp (Ctenopharyngodon idellus) SO PARASITOLOGY RESEARCH LA English DT Article DE Ichthyophthirius multifiliis; Cynanchum atratum; Zingiber officinale; Cynanchumpaniculatum; Immunostimulants; Medicated feed ID GOLDFISH CARASSIUS-AURATUS; GINGER ZINGIBER-OFFICINALE; CYNANCHUM-ATRATUM; AEROMONAS-HYDROPHILA; PREGNANE GLYCOSIDES; CYPRINUS-CARPIO; COPPER-SULFATE; RAINBOW-TROUT; LABEO-ROHITA; SODIUM PERCARBONATE AB Since malachite green was banned for using in food fish due to its carcinogenic and teratogenic effects on human, the search of alternative drug to treat Ichthyophthirius multifiliis becomes urgent. This study aimed to (1) evaluate the ethanol extracts of medicinal plants Cynanchum atratum, Zingiber officinale, Cynanchum paniculatum, immunostimulant (A), and immunostimulant (B) for their efficacy against I. multifiliis, and (2) determine effects of medicated feeds with C. atratum, Z. officinale, C. paniculatum, and immunostimulant (A) to treat I. multifiliis in grass carp. The results in this study showed that the minimum concentrations of C. atratum, Z. officinale, and C. paniculatum extracts for killing all theronts were 16, 8, and 16 mg/L, respectively. In vivo experiments, fish fed with medicated feeds of C. atratum for 10 days, or Z. officinale for 3 days, or combination of three plants for 10 days resulted in a significant reduction in the I. multifiliis infective intensity on grass carp after theronts exposure. Grass carp fed with medicated feeds of immunostimulant (A) for 21 days showed no infection and 100 % of survival 15 days post theronts exposure. Therefore, immunostimulant (A) is a promising feed supplement to treated I. multifiliis with good antiparasitic efficacy. C1 [Lin, De-Jie; Hua, Ya-Nan; Zhang, Qi-Zhong; Fu, Yao-Wu; Liu, Yan-Meng; Zhou, Sheng-Yu] Jinan Univ, Minist Educ, Key Lab Eutrophicat & Red Tide Prevent Guangdong, Engn Res Ctr Trop & Subtrop Aquat Ecol Engn,Inst, Guangzhou 510632, Guangdong, Peoples R China. [Xu, De-Hai] ARS, USDA, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 USA. RP Zhang, QZ (reprint author), Jinan Univ, Minist Educ, Key Lab Eutrophicat & Red Tide Prevent Guangdong, Engn Res Ctr Trop & Subtrop Aquat Ecol Engn,Inst, Guangzhou 510632, Guangdong, Peoples R China. EM zhangqzdr@126.com FU National High Technology Research and Development Program of China (863 Program) [2011AA10A216]; Marine and Fishery Special Project of Science and Technology in Guangdong Province [A201301B05, A201501B09]; Guangzhou Science and Technology Project [2013J4100047] FX This work was supported by the National High Technology Research and Development Program of China (863 Program) (No. 2011AA10A216), the Marine and Fishery Special Project of Science and Technology in Guangdong Province (A201301B05, A201501B09), and Guangzhou Science and Technology Project (No. 2013J4100047). NR 54 TC 0 Z9 0 U1 9 U2 14 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 EI 1432-1955 J9 PARASITOL RES JI Parasitol. Res. PD JUN PY 2016 VL 115 IS 6 BP 2473 EP 2483 DI 10.1007/s00436-016-5000-y PG 11 WC Parasitology SC Parasitology GA DM1JH UT WOS:000376101000038 PM 27003405 ER PT J AU Nachman, RJ AF Nachman, Ronald J. TI Invertebrate Neuropeptides XV Introduction SO PEPTIDES LA English DT Editorial Material C1 [Nachman, Ronald J.] USDA, Southern Plains Agr Res Ctr, Insect Neuropeptide Lab, Insect Control & Cotton Dis Res, 2881 F&B Rd, College Stn, TX 77845 USA. RP Nachman, RJ (reprint author), USDA, Southern Plains Agr Res Ctr, Insect Neuropeptide Lab, Insect Control & Cotton Dis Res, 2881 F&B Rd, College Stn, TX 77845 USA. EM nachman@tamu.edu NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0196-9781 EI 1873-5169 J9 PEPTIDES JI Peptides PD JUN PY 2016 VL 80 BP 1 EP 3 DI 10.1016/j.peptides.2016.04.003 PG 3 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA DL9BM UT WOS:000375936400001 PM 27198499 ER PT J AU Lange, AB Nachman, RJ Kaczmarek, K Zabrocki, J AF Lange, Angela B. Nachman, Ronald J. Kaczmarek, Krzysztof Zabrocki, Janusz TI Biostable insect kinin analogs reduce blood meal and disrupt ecdysis in the blood-gorging Chagas' disease vector, Rhodnius prolixus SO PEPTIDES LA English DT Article; Proceedings Paper CT Invertebrate Neuropeptide Conference 2015 (INC2015). CY 2015 CL Bagan, MYANMAR DE Diuresis; Blood-feeding; Molting; Neuropeptide; Kinin; Peptide ID TICK BOOPHILUS-MICROPLUS; MOSQUITO AEDES-AEGYPTI; LEUCOPHAEA-MADERAE; NEUROPEPTIDE FAMILY; APHICIDAL ACTIVITY; RECEPTORS; PEPTIDES; MEMBERS; CEPHALOMYOTROPINS; IDENTIFICATION AB Rhodnius prolixus is a blood-gorging hemipteran that takes blood meals that are approximately 10 times its body weight. This blood meal is crucial for growth and development and is needed to ensure a successful molt into the next instar. Kinins are a multifunctional family of neuropeptides which have been shown to play a role in the control of feeding in a variety of insects. In this study, two biostable Aib-containing kinin analogs were tested to see if they interfere with blood-feeding and subsequent development into the next instar. One of the analogs, 1729 (Ac-R[Aib]FF[Aib]WGa), had no effect on the size of the blood meal or on the subsequent molting of the insect into the next instar. This analog also did not interfere with either short-term or long-term diuresis. The second analog, 1728 ([Aib]FF[Aib]WGa), appeared to be an antifeedant. Insects feeding on blood containing this analog (15 mu M) only consumed 60% of the blood meal taken by insects fed on blood without analog. Insects feeding on blood containing 1728 had a slower rate of rapid diuresis (diuresis in the first 3-5 h after feeding) leading to less urine being excreted by 5 days post feeding. The consequence of these effects was that insects fed on 1728 did not molt. This data indicates that the biostable Aib-containing analog 1728 disrupts normal growth and development in the blood-feeding insect, R. prolixus. (C) 2016 Elsevier Inc. All rights reserved. C1 [Lange, Angela B.] Univ Toronto, Dept Biol, Mississauga, ON L5L 1C6, Canada. [Nachman, Ronald J.; Kaczmarek, Krzysztof; Zabrocki, Janusz] USDA, Insect Control & Cotton Dis Res, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. [Kaczmarek, Krzysztof; Zabrocki, Janusz] Lodz Univ Technol, Inst Organ Chem, PL-90924 Lodz, Poland. RP Lange, AB (reprint author), Univ Toronto, Dept Biol, Mississauga, ON L5L 1C6, Canada. EM angela.lange@utoronto.ca FU NSERC; USDA/DOD DWFP [60-0208-4-001] FX This work was funded by an NSERC Discovery Grant to A.B.L. and a USDA/DOD DWFP (60-0208-4-001) grant to RJ.N. We are grateful to Himali Patel for help in feeding the insects and Nikki Sarkar for maintaining the R. prolixus colony. NR 33 TC 1 Z9 1 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0196-9781 EI 1873-5169 J9 PEPTIDES JI Peptides PD JUN PY 2016 VL 80 BP 108 EP 113 DI 10.1016/j.peptides.2016.01.012 PG 6 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA DL9BM UT WOS:000375936400014 PM 26807486 ER PT J AU Stommel, JR Camp, MJ Luo, YG Welten-Schoevaars, AM AF Stommel, John R. Camp, Mary J. Luo, Yaguang Welten-Schoevaars, Anne Marie TI Genetic diversity provides opportunities for improvement of fresh-cut pepper quality SO PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION LA English DT Article DE Capsicum; electrolyte leakage; germplasm; modified atmosphere packaging; quality; shelf life ID POSTHARVEST BIOLOGY; SENSORY QUALITY; SHELF-LIFE; VEGETABLES; CARROTS; SLICES; LEAVES; FRUITS; OXYGEN AB Genetic diversity identified in the Capsicum genepool has been utilized extensively to improve pepper disease resistance, fruit quality and varied yield attributes. Little attention has been dedicated to evaluating the breadth of potential diversity within Capsicum for fresh-cut attributes and genetic enhancement of fresh-cut fruit quality. We evaluated fresh-cut attributes in pepper accessions with diverse fruit phenotype selected from available cultivars and the USDA, ARS Capsicum genebank. Subjective assessment of product quality and objective measurement of package atmospheric composition, tissue juice leakage and membrane electrolyte leakage after 7, 10 and 14d of storage identified significant differences for fresh-cut attributes among as well as within sweet bell, large elongate, jalapeno and serrano germplasm. Sweet bell and large elongate fruited accessions generally exhibited increasing electrolyte leakage over days of storage, whereas jalapeno and serrano accessions maintained stable electrolyte leakage levels. Jalapeno and serrano fruit classes were typified by faster decline in package headspace O-2 and accumulation in CO2 partial pressures in comparison to sweet bell and large elongated fruit classes. Regression analysis demonstrated a relationship between overall visual quality and electrolyte leakage after 14d of storage for sweet bell and large elongated fruit classes. The results demonstrate extensive variation in Capsicum germplasm to improve pepper for fresh-cut applications and facilitate research to better understand physiological and heritable determinants of fresh-cut product quality. C1 [Stommel, John R.] USDA ARS, Genet Improvement Fruits & Vegetables Lab, B-010A,Beltsville Agr Res Ctr West, Beltsville, MD 20705 USA. [Camp, Mary J.] USDA ARS, Beltsville Agr Res Ctr, Biometr Consulting Serv, Beltsville, MD 20705 USA. [Luo, Yaguang] USDA ARS, Beltsville Agr Res Ctr, Food Qual Lab, Beltsville, MD 20705 USA. [Welten-Schoevaars, Anne Marie] Enza Zaden Res USA Inc, 525 Lucy Brown Lane, San Juan Bautista, CA 95045 USA. RP Stommel, JR (reprint author), USDA ARS, Genet Improvement Fruits & Vegetables Lab, B-010A,Beltsville Agr Res Ctr West, Beltsville, MD 20705 USA. EM john.stommel@ars.usda.gov NR 33 TC 1 Z9 1 U1 4 U2 8 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1479-2621 EI 1479-263X J9 PLANT GENET RESOUR-C JI Plant Genet. Resour.-Charact. Util. PD JUN PY 2016 VL 14 IS 2 BP 112 EP 120 DI 10.1017/S1479262115000131 PG 9 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA DM4BQ UT WOS:000376291000004 ER PT J AU Markowski-Lindsay, M Catanzaro, P Milman, A Kittredge, D AF Markowski-Lindsay, Marla Catanzaro, Paul Milman, Anita Kittredge, David TI Understanding Family Forest Land Future Ownership and Use: Exploring Conservation Bequest Motivations SO SMALL-SCALE FORESTRY LA English DT Article DE Land protection; Land transfer; Non-industrial private forest owner (NIPF); Life-cycle ID PLACE ATTACHMENT; INTENTIONS; LANDOWNERS; EASEMENTS AB Due to an aging landowner population, there will be an unprecedented ownership shift in land-based assets in the USA. Approximately 2.7 million family forest owners (FFOs) in the USA over the age of 55 years old, reflecting 80 % of all FFO-owned land, will be deciding the future ownership and use of their land, having significant implications for the landscapes and public benefits these forests provide. Little is known about how FFOs plan for the future ownership and use of their land. This study draws from life-cycle theory and FFO decision cycle research to propose a future ownership and use decision-making framework and to explore the presence of and motivation for FFOs to make "conservation bequests" designed to maintain land in its current, forested form. Qualitative interviews with professionals working with over 1000 FFOs explore what triggers FFOs to consider their options and influence their decisions. Findings suggest triggers are universally-held (e.g. age, health) and can happen any time in an FFO's life. FFO decisions are complex and often made in the context of family goals and dynamics. FFOs holding deep attachments to their land may be more likely to make conservation bequests. However, despite conservation preferences, due to the complex nature of the process or external influences, decisions may not always lead to conservation bequests. The framework that this study adopts allows future in-depth research on these critical decisions. In particular, additional research with landowners is required to examine the framework in more detail. C1 [Markowski-Lindsay, Marla; Kittredge, David] Univ Massachusetts, Family Forest Res Ctr, USDA Forest Serv, Holdsworth Way, Amherst, MA 01003 USA. [Catanzaro, Paul; Milman, Anita; Kittredge, David] Univ Massachusetts, Dept Environm Conservat, Holdsworth Way, Amherst, MA 01003 USA. RP Markowski-Lindsay, M (reprint author), Univ Massachusetts, Family Forest Res Ctr, USDA Forest Serv, Holdsworth Way, Amherst, MA 01003 USA. EM marla@eco.umass.edu FU National Institute of Food and Agriculture (NIFA); US Department of Agriculture (USDA); Massachusetts Agricultural Experiment Station; Department of Environmental Conservation at the University of Massachusetts Amherst [MAS 000017] FX We would like to thank Wendy Ferris and Jay Rasku for their interviewing efforts as well as the interview participants themselves who provided their insights into the estate planning process. This material is based upon work supported by the National Institute of Food and Agriculture (NIFA), US Department of Agriculture (USDA), Massachusetts Agricultural Experiment Station and Department of Environmental Conservation at the University of Massachusetts Amherst, under Project Number MAS 000017. The contents are solely the responsibility of the authors and do not necessarily represent the official views of the USDA or NIFA. NR 42 TC 0 Z9 0 U1 6 U2 9 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1873-7617 EI 1873-7854 J9 SMALL-SCALE FOR JI Small-Scale For. PD JUN PY 2016 VL 15 IS 2 BP 241 EP 256 DI 10.1007/s11842-015-9320-z PG 16 WC Forestry SC Forestry GA DM3WB UT WOS:000376276300008 ER PT J AU Islam, MS Zeng, LH Thyssen, GN Delhom, CD Kim, HJ Li, P Fang, DD AF Islam, Md. S. Zeng, Linghe Thyssen, Gregory N. Delhom, Christopher D. Kim, Hee Jin Li, Ping Fang, David D. TI Mapping by sequencing in cotton (Gossypium hirsutum) line MD52ne identified candidate genes for fiber strength and its related quality attributes SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID QUANTITATIVE TRAIT LOCI; CELL-WALL DEVELOPMENT; UPLAND COTTON; BUNDLE STRENGTH; QTL ANALYSIS; L.; ARABIDOPSIS; POPULATION; BARBADENSE; EXPRESSION AB Three QTL regions controlling three fiber quality traits were validated and further fine-mapped with 27 new single nucleotide polymorphism (SNP) markers. Transcriptome analysis suggests that receptor-like kinases found within the validated QTLs are potential candidate genes responsible for superior fiber strength in cotton line MD52ne. Fiber strength, length, maturity and fineness determine the market value of cotton fibers and the quality of spun yarn. Cotton fiber strength has been recognized as a critical quality attribute in the modern textile industry. Fine mapping along with quantitative trait loci (QTL) validation and candidate gene prediction can uncover the genetic and molecular basis of fiber quality traits. Four previously-identified QTLs (qFBS-c3, qSFI-c14, qUHML-c14 and qUHML-c24) related to fiber bundle strength, short fiber index and fiber length, respectively, were validated using an F-3 population that originated from a cross of MD90ne x MD52ne. A group of 27 new SNP markers generated from mapping-by-sequencing (MBS) were placed in QTL regions to improve and validate earlier maps. Our refined QTL regions spanned 4.4, 1.8 and 3.7 Mb of physical distance in the Gossypium raimondii reference genome. We performed RNA sequencing (RNA-seq) of 15 and 20 days post-anthesis fiber cells from MD52ne and MD90ne and aligned reads to the G. raimondii genome. The QTL regions contained 21 significantly differentially expressed genes (DEGs) between the two near-isogenic parental lines. SNPs that result in non-synonymous substitutions to amino acid sequences of annotated genes were identified within these DEGs, and mapped. Taken together, transcriptome and amino acid mutation analysis indicate that receptor-like kinase pathway genes are likely candidates for superior fiber strength and length in MD52ne. MBS along with RNA-seq demonstrated a powerful strategy to elucidate candidate genes for the QTLs that control complex traits in a complex genome like tetraploid upland cotton. C1 [Islam, Md. S.; Kim, Hee Jin; Li, Ping; Fang, David D.] USDA ARS, So Reg Res Ctr, Cotton Fiber Biosci Res Unit, POB 19687, New Orleans, LA 70124 USA. [Zeng, Linghe] USDA ARS, Crop Genet Res Unit, 141 Expt Stn Rd, Stoneville, MS 38772 USA. [Thyssen, Gregory N.] USDA ARS, So Reg Res Ctr, Cotton Chem & Utilizat Res Unit, POB 19687, New Orleans, LA 70124 USA. [Delhom, Christopher D.] USDA ARS, So Reg Res Ctr, Cotton Struct & Qual Res Unit, POB 19687, New Orleans, LA 70124 USA. RP Fang, DD (reprint author), USDA ARS, So Reg Res Ctr, Cotton Fiber Biosci Res Unit, POB 19687, New Orleans, LA 70124 USA. EM david.fang@ars.usda.gov FU USDA-ARS CRIS [6054-21000-017-00D] FX This project was supported by USDA-ARS CRIS project 6054-21000-017-00D. Our appreciation goes to Data-2Bio and BGI Americas for their excellent support in library construction, genome wide RNA and DNA sequencing, respectively. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture that is an equal opportunity provider and employer. NR 64 TC 4 Z9 4 U1 6 U2 20 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JUN PY 2016 VL 129 IS 6 BP 1071 EP 1086 DI 10.1007/s00122-016-2684-4 PG 16 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DM3MK UT WOS:000376250300001 PM 26883043 ER PT J AU Burton, AL Burkey, KO Carter, TE Orf, J Cregan, PB AF Burton, Amy L. Burkey, Kent O. Carter, Thomas E., Jr. Orf, James Cregan, Perry B. TI Phenotypic variation and identification of quantitative trait loci for ozone tolerance in a Fiskeby III x Mandarin (Ottawa) soybean population SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID CARBON-DIOXIDE ENRICHMENT; PHASEOLUS-VULGARIS L.; ZEA-MAYS L.; ELEVATED OZONE; CLIMATE-CHANGE; ASCORBIC-ACID; STOMATAL CONDUCTANCE; TROPOSPHERIC OZONE; GENE-EXPRESSION; GRAIN QUALITY AB Soybean quantitative trait loci for ozone response. Ground-level ozone reduces yield in crops such as soybean (Glycine max (L.) Merr.). Phenotypic variation has been observed for this trait in multiple species; however, breeding for ozone tolerance has been limited. A recombinant inbred population was developed from soybean genotypes differing in tolerance to ozone: tolerant Fiskeby III and sensitive Mandarin (Ottawa). Plants were exposed to ozone treatment for 5 days in greenhouse chambers followed by visual scoring for foliar injury. Mean injury score in the mid-canopy was 16 % for Fiskeby III, and 81 % for Mandarin (Ottawa). Injury scores were lower in younger leaves for both parents and progeny, compared to scores in the older leaves. Segregation was consistent with multigenic inheritance. Correlation coefficients for injury between leaf positions ranged from 0.34 to 0.81, with the closer leaf positions showing the greater correlation. Narrow sense heritability within an ozone treatment chamber was 0.59, 0.40, 0.29, 0.30, 0.19, and 0.35 for the 2nd, 3rd, 4th, 5th, 6th, and combined 3rd-5th main stem leaf positions (numbered acropetally), respectively, based on genotypic means over three independent replications. Quantitative trait loci (QTL) analysis showed that loci were associated with distinct leaf developmental stages. QTL were identified on Chromosome 17 for the 2nd and 3rd leaf positions, and on Chromosome 4 for the 5th and 6th leaf positions. Additional loci were identified on Chromosomes 6, 18, 19, and 20. Interacting loci were identified on Chromosomes 5 and 15 for injury on trifoliate 4. The ozone sensitive parent contributed one favorable allele for ozone response. C1 [Burton, Amy L.; Burkey, Kent O.] USDA ARS, Plant Sci Res Unit, 3127 Ligon St, Raleigh, NC 27607 USA. [Carter, Thomas E., Jr.] USDA ARS, Soybean & Nitrogen Fixat Unit, 3127 Ligon St, Raleigh, NC 27607 USA. [Burkey, Kent O.; Carter, Thomas E., Jr.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Orf, James] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Cregan, Perry B.] USDA ARS, Soybean Genom & Improvement Lab, 10300 Baltimore Ave,Bldg 006,BARC West, Beltsville, MD 20705 USA. RP Burkey, KO (reprint author), USDA ARS, Plant Sci Res Unit, 3127 Ligon St, Raleigh, NC 27607 USA.; Burkey, KO (reprint author), N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. EM Kent.Burkey@ars.usda.gov FU United Soybean Board [0286, 1320-532-5610] FX This work was supported by Grants #0286 and #1320-532-5610 from the United Soybean Board. NR 54 TC 0 Z9 0 U1 5 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JUN PY 2016 VL 129 IS 6 BP 1113 EP 1125 DI 10.1007/s00122-016-2687-1 PG 13 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DM3MK UT WOS:000376250300004 PM 26920548 ER PT J AU Roach, JA Verma, S Peres, NA Jamieson, AR van de Weg, WE Bink, MCAM Bassil, NV Lee, S Whitaker, VM AF Roach, Jack A. Verma, Sujeet Peres, Natalia A. Jamieson, Andrew R. van de Weg, W. Eric Bink, Marco C. A. M. Bassil, Nahla V. Lee, Seonghee Whitaker, Vance M. TI FaRXf1: a locus conferring resistance to angular leaf spot caused by Xanthomonas fragariae in octoploid strawberry SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID PLANT-POPULATIONS; DISEASE; LINKAGE; STRAINS; APPLE; IDENTIFICATION; PRIMERS; PROGRAM AB Angular leaf spot is a devastating bacterial disease of strawberry. Resistance from two wild accessions is highly heritable and controlled by a major locus on linkage group 6D. Angular leaf spot caused by Xanthomonas fragariae is the only major bacterial disease of cultivated strawberry (Fragaria xananassa). While this disease may cause reductions of up to 8 % of marketable yield in Florida winter annual production, no resistant cultivars have been commercialized. Wild accessions US4808 and US4809 were previously identified as resistant to the four genetic clades of X. fragariae, and introgression of the trait into commercial quality perennial-type germplasm was initiated. Previous reports indicated high heritability for the trait but proposed both single-locus and multi-locus inheritance models. The objective of this study was to determine the mode of inheritance of resistance, to identify causal loci, and to begin introgression of resistance into Florida-adapted germplasm. Resistance was observed in two years of field trials with inoculated plants that assayed four full-sib families descended from US4808 to US4809. Resistance segregated 1:1 in all families indicating control by a dominant allele at a single locus. Using a selective genotyping approach with the IStraw90 Axiom(A (R)) SNP array and pedigree-based QTL detection, a single major-effect QTL was identified in two full-sib families, one descended from each resistant accession. High-resolution melt curve analysis validated the presence of the QTL in separate populations. The QTL was delimited to the 33.1-33.6 Mbp (F. vesca vesca v1.1 reference) and 34.8-35.3 Mbp (F. vesca bracteata v2.0 reference) regions of linkage group 6D for both resistance sources and was designated FaRXf1. Characterization of this locus will facilitate marker-assisted selection toward the development of resistant cultivars. C1 [Roach, Jack A.; Verma, Sujeet; Lee, Seonghee; Whitaker, Vance M.] Univ Florida, IFAS Gulf Coast Res & Educ Ctr, Dept Hort Sci, 14625 CR 672, Wimauma, FL 33598 USA. [Peres, Natalia A.] Univ Florida, IFAS Gulf Coast Res & Educ Ctr, Dept Plant Pathol, 14625 CR 672, Wimauma, FL 33598 USA. [Jamieson, Andrew R.] Agr & Agri Food Canada, Atlantic Food & Hort Res Ctr, 32 Main St, Kentville, NS B4N 1J5, Canada. [van de Weg, W. Eric] Wageningen UR Plant Breeding, POB 16, NL-6700 AA Wageningen, Netherlands. [Bink, Marco C. A. M.] Wageningen Univ & Res Ctr, Biometris, POB 16, NL-6700 AA Wageningen, Netherlands. [Bassil, Nahla V.] USDA ARS, Natl Clonal Germplasm Repository, 33447 Peoria Rd, Corvallis, OR 97333 USA. RP Whitaker, VM (reprint author), Univ Florida, IFAS Gulf Coast Res & Educ Ctr, Dept Hort Sci, 14625 CR 672, Wimauma, FL 33598 USA. EM vwhitaker@ufl.edu RI Van de Weg, Eric/G-3225-2011 OI Van de Weg, Eric/0000-0002-9443-5974 FU National Institute of Food and Agriculture, U.S. Department of Agriculture Specialty Crop Research Initiative [2014-51181-22378] FX The authors acknowledge Tomas Hasing, Dr. James Mertely, and Teresa Seijo for their assistance and guidance. This work was funded in part by the National Institute of Food and Agriculture, U.S. Department of Agriculture Specialty Crop Research Initiative project "RosBREED: Combining disease resistance with horticultural quality in new rosaceous cultivars" under award No 2014-51181-22378. NR 43 TC 2 Z9 2 U1 11 U2 20 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JUN PY 2016 VL 129 IS 6 BP 1191 EP 1201 DI 10.1007/s00122-016-2695-1 PG 11 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DM3MK UT WOS:000376250300010 PM 26910360 ER PT J AU Stokdyk, JP Firnstahl, AD Spencer, SK Burch, TR Borchardt, MA AF Stokdyk, Joel P. Firnstahl, Aaron D. Spencer, Susan K. Burch, Tucker R. Borchardt, Mark A. TI Determining the 95% limit of detection for waterborne pathogen analyses from primary concentration to qPCR SO WATER RESEARCH LA English DT Article DE Limit of detection; Quantitative PCR; Environmental pathogen detection ID REAL-TIME PCR; MICROBIAL SOURCE TRACKING; QUANTITATIVE PCR; SEWAGE CONTAMINATION; SALMONELLA-ENTERICA; FECAL POLLUTION; GENETIC-MARKER; FRESH-WATER; SAMPLES; ASSAYS AB The limit of detection (LOD) for qPCR-based analyses is not consistently defined or determined in studies on waterborne pathogens. Moreover, the LODs reported often reflect the qPCR assay alone rather than the entire sample process. Our objective was to develop an approach to determine the 95% LOD (lowest concentration at which 95% of positive samples are detected) for the entire process of waterborne pathogen detection. We began by spiking the lowest concentration that was consistently positive at the qPCR step (based on its standard curve) into each procedural step working backwards (i.e., extraction, secondary concentration, primary concentration), which established a concentration that was detectable following losses of the pathogen from processing. Using the fraction of positive replicates (n = 10) at this concentration, we selected and analyzed a second, and then third, concentration. If the fraction of positive replicates equaled 1 or 0 for two concentrations, we selected another. We calculated the LOD using probit analysis. To demonstrate our approach we determined the 95% LOD for Salmonella enterica serovar Typhimurium, adenovirus 41, and vaccine-derived poliovirus Sabin 3, which were 11, 12, and 6 genomic copies (gc) per reaction (rxn), respectively (equivalent to 13, 1.5, and 4.0 gc L-1 assuming the 1500 L tap-water sample volume prescribed in EPA Method 1615). This approach limited the number of analyses required and was amenable to testing multiple genetic targets simultaneously (i.e., spiking a single sample with multiple microorganisms). An LOD determined this way can facilitate study design, guide the number of required technical replicates, aid method evaluation, and inform data interpretation. Published by Elsevier Ltd. C1 [Stokdyk, Joel P.; Firnstahl, Aaron D.] US Geol Survey, Wisconsin Water Sci Ctr, Middleton, WI USA. [Spencer, Susan K.; Burch, Tucker R.; Borchardt, Mark A.] ARS, Environmentally Integrated Dairy Management Res U, USDA, Marshfield, WI USA. [Stokdyk, Joel P.; Firnstahl, Aaron D.; Spencer, Susan K.; Burch, Tucker R.; Borchardt, Mark A.] Lab Infect Dis & Environm, Marshfield, WI USA. RP Borchardt, MA (reprint author), 2615 Yellowstone Dr Agr Res Serv, USDA, Marshfield, WI 54449 USA. EM mark.borchardt@ars.usda.gov OI Stokdyk, Joel/0000-0003-2887-6277 NR 47 TC 0 Z9 0 U1 7 U2 18 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0043-1354 J9 WATER RES JI Water Res. PD JUN 1 PY 2016 VL 96 BP 105 EP 113 DI 10.1016/j.watres.2016.03.026 PG 9 WC Engineering, Environmental; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA DM2XP UT WOS:000376211700011 PM 27023926 ER PT J AU Yeh, HY Hiett, KL Line, JE Jagne, JF Lauer, DC AF Yeh, H. -Y. Hiett, K. L. Line, J. E. Jagne, J. F. Lauer, D. C. TI Seroprevalence in Chickens against Campylobacter jejuni Flagellar Capping Protein (FliD) in Selected Areas of the United States SO ZOONOSES AND PUBLIC HEALTH LA English DT Article DE Campylobacter jejuni; FliD; flagellar capping protein; food-borne pathogen; zoonosis ID HOOK-ASSOCIATED PROTEINS; MOLECULAR CHARACTERIZATION; SALMONELLA-TYPHIMURIUM; CLOSTRIDIUM-DIFFICILE; HELICOBACTER; EXPRESSION; SEQUENCE; GENE; ANTIGENICITY; POULTRY AB Campylobacter jejuni is a causative pathogen of human acute bacterial gastroenteritis. Infected poultry products are regarded as a major source for human C. jejuni infection. The flagellar capping protein (FliD) is highly conserved among C. jejuni strains/isolates and is antigenic as analysed by immunoblot. In this study, we used the FliD protein as a probe to survey the prevalence of C. jejuni antibodies in chickens from two areas in the United States. A total of 394 samples were tested. Sera from layer breeders of 44-52weeks of age tested 100% positive, while 4- to 6-week broilers from 22 premises showed 7-100% positivity. These results demonstrate that anti-FliD antibodies were prevalent in the poultry population in the areas of serum samples collected. C1 [Yeh, H. -Y.; Hiett, K. L.; Line, J. E.] ARS, USDA, US Natl Poultry Res Ctr, Poultry Microbiol Safety & Proc Res Unit, 950 Coll Stn Rd, Athens, GA 30605 USA. [Jagne, J. F.] Cornell Univ, Anim Hlth Diagnost Ctr, Ithaca, NY USA. [Lauer, D. C.] Univ Minnesota, Minnesota Poultry Testing Lab, Willmar, MN USA. RP Yeh, HY (reprint author), ARS, USDA, US Natl Poultry Res Ctr, Poultry Microbiol Safety & Proc Res Unit, 950 Coll Stn Rd, Athens, GA 30605 USA. EM hungyueh.yeh@ars.usda.gov FU USDA Agricultural Research Service CRIS Project [6612-32000-060-00]; U.S. Poultry & Egg Association [679] FX We are grateful to Susan Q. Brooks of the Poultry Microbiological Safety and Processing Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Athens, GA for excellent technical support. We appreciate Stacy Pollock and Shauna Voss of the Minnesota Poultry Testing Laboratory, University of Minnesota, Willmar, MN for providing chicken sera. We also thank Jennifer Yeh of Johns Hopkins School of Medicine for critical reading of this manuscript. This study was supported by the USDA Agricultural Research Service CRIS Project No. 6612-32000-060-00 and the U.S. Poultry & Egg Association Project No. 679. Mention of trade names or commercial products in this study is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The U.S. Department of Agriculture is an equal opportunity provider and employer. NR 33 TC 1 Z9 1 U1 3 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1863-1959 EI 1863-2378 J9 ZOONOSES PUBLIC HLTH JI Zoonoses Public Health PD JUN PY 2016 VL 63 IS 4 BP 265 EP 270 DI 10.1111/zph.12237 PG 6 WC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences SC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences GA DM3TJ UT WOS:000376269300002 PM 26603949 ER PT J AU Hummel, SS AF Hummel, Susan Stevens TI Caring for place: ecology, ideology, and emotion in traditional landscape management SO AGRICULTURE AND HUMAN VALUES LA English DT Book Review C1 [Hummel, Susan Stevens] US Forest Serv, USDA, Portland Forestry Sci Lab, 620 SW Main St, Portland, OR 97205 USA. RP Hummel, SS (reprint author), US Forest Serv, USDA, Portland Forestry Sci Lab, 620 SW Main St, Portland, OR 97205 USA. EM shummel@fs.fed.us NR 1 TC 0 Z9 0 U1 2 U2 4 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0889-048X EI 1572-8366 J9 AGR HUM VALUES JI Agric. Human Values PD JUN PY 2016 VL 33 IS 2 BP 495 EP 496 DI 10.1007/s10460-016-9691-6 PG 2 WC Agriculture, Multidisciplinary; History & Philosophy Of Science; Sociology SC Agriculture; History & Philosophy of Science; Sociology GA DL5WE UT WOS:000375706900021 ER PT J AU Milbrath, LR Biazzo, J AF Milbrath, Lindsey R. Biazzo, Jeromy TI Impact of the defoliating moth Hypena opulenta on invasive swallow-worts (Vincetoxicum species) under different light environments SO BIOLOGICAL CONTROL LA English DT Article DE Cynanchum rossicum; Cynanchum louiseae; Swallow-wort; Dog-strangling vine; Agent impact; Weed biological control ID BIOLOGICAL-CONTROL AGENT; NEW-YORK-STATE; RELEASE EFFICACY ASSESSMENT; NORTH-AMERICA; SIMULATED HERBIVORY; HOST-SPECIFICITY; ALLIARIA-PETIOLATA; MACARIA-PALLIDATA; DELAIREA-ODORATA; SEED PRODUCTION AB Black and pale swallow-wort (Vincetoxicum nigrum and Vincetoxicum rossicum, Apocynaceae) are twining vines from Europe that have become invasive in eastern North America. Hypena opulenta (Lepidoptera: Erebidae), a defoliating moth from the Ukraine, has recently been released in Canada and a request for release in the United States is under review. Its potential multivoltinism, and hence repeated defoliation of swallow-worts, combined with low-light levels in an understory environment may be promising for controlling forest infestations of swallow-worts. We conducted a greenhouse study of the impact of larval defoliation on seedlings and mature (flowering) plants of black and pale swallow-wort at different frequencies (once or twice) and degrees of defoliation (50 or 100%-seedlings; three or six larvae per stem-mature plants). Defoliation occurred under two forest light-regimes: low-light conditions typical of deeply-shaded forest habitats and higher light levels representative of a more open canopy or forest edge. Plants grown under higher light conditions were generally larger and produced many more seeds than heavily-shaded plants. One round of complete defoliation by H. opulenta, but not necessarily two, generally reduced biomass and seed production for both species and life stages, except for root mass of mature plants. No mature plants died and little seedling mortality occurred apart from black swallow-wort that had been completely defoliated twice under low light. Pale and black swallow-wort seedlings and mature plants appear to be tolerant of moderate defoliation damage. Complete defoliation repeated within and across years may be needed for suppression of forest infestations of swallow-wort. Published by Elsevier Inc. C1 [Milbrath, Lindsey R.; Biazzo, Jeromy] ARS, USDA, Robert W Holley Ctr Agr & Hlth, 538 Tower Rd, Ithaca, NY 14853 USA. RP Milbrath, LR (reprint author), ARS, USDA, Robert W Holley Ctr Agr & Hlth, 538 Tower Rd, Ithaca, NY 14853 USA. EM Lindsey.Milbrath@ars.usda.gov NR 83 TC 0 Z9 0 U1 9 U2 16 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD JUN PY 2016 VL 97 BP 1 EP 12 DI 10.1016/j.biocontrol.2016.02.009 PG 12 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DJ9CL UT WOS:000374511500001 ER PT J AU Wraight, SP Ugine, TA Ramos, ME Sanderson, JP AF Wraight, S. P. Ugine, T. A. Ramos, M. E. Sanderson, J. P. TI Efficacy of spray applications of entomopathogenic fungi against western flower thrips infesting greenhouse impatiens under variable moisture conditions SO BIOLOGICAL CONTROL LA English DT Article DE Beauveria bassiana; Metarhizium spp.; Greenhouse efficacy; Frankliniella occidentalis; Moisture conditions; Relative humidity ID FRANKLINIELLA-OCCIDENTALIS PERGANDE; TRIALEURODES-VAPORARIORUM HOMOPTERA; BEAUVERIA-BASSIANA BALSAMO; HOST-PLANT; PAECILOMYCES-FUMOSOROSEUS; METARHIZIUM-ANISOPLIAE; BEMISIA-ARGENTIFOLII; RHODNIUS-PROLIXUS; GROWING MEDIA; THYSANOPTERA AB Laboratory bioassays of three entomopathogenic fungi (Beauveria bassiana strain GHA, Metarhizium brunneum strain F52, and Metarhizium anisopliae s.l. strain ESC-1) were conducted against 2nd-instar nymphs of Frankliniella occidentalis. All three fungi were highly virulent, with respective LC(50)s of 193, 140, and 72 conidia/mm(2) of treated leaf surface. Efficacy tests were conducted against thrips infesting small plots of Impatiens walleriana on open greenhouse benches under variable moisture conditions. Multiple spray applications of fungal conidia suspended in 0.01% Silwet (R) were made at a high rate of 2 x 10(14) conidia/ha at 3-5-day intervals, and efficacy was assessed from twice-weekly samples of both flowers and foliage. During tests, moisture conditions were modified via controlled sprays of water onto the concrete floor beneath the greenhouse benches for a period of ca. 40 h (from the evening through the second night) following each application (post-spray period). Efficacy of the pathogens expressed as percent reduction of the combined populations of thrips nymphs and adults relative to spray-carrier controls was ultimately correlated to greenhouse environmental conditions. Mean relative humidity (RH) over all test days was a strong predictor of thrips control on foliage (R-2 = 0.820) and flowers (R-2 = 0.756). Fungal efficacy was most strongly and consistently predicted by moisture conditions during the post-spray periods. The single best predictor was mean number of hours during post-spray periods when RH was >= 80% (R-2 foliage = 0.834; R-2 flowers = 0.908). The three fungi produced similar levels of thrips control under comparable moisture conditions. Maintenance of 80% RH for ca. 25 h during post-spray periods resulted in >70% control of thrips in foliage. Substantially longer periods of high humidity (35 h) were required to achieve comparable control in flowers. Control >= 70% in both foliage and flowers prevented pest populations from increasing. Aspects of the impatiens crop that create a challenge for thrips control using fungal entomopathogens are described and discussed. (C) 2016 Published by Elsevier Inc. C1 [Wraight, S. P.] ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Ugine, T. A.; Sanderson, J. P.] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. [Ramos, M. E.] ARS, USDA, RW Holley Ctr, Ithaca, NY 14853 USA. RP Wraight, SP (reprint author), ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM steve.wraight@ars.usda.gov FU Floral and Nursery Crops Research Initiative of USDA-ARS FX This study would not have been possible without the excellent technical assistance of Rebecca Howes. This research was funded, in part, by the Floral and Nursery Crops Research Initiative of USDA-ARS. Thanks to Sandra Galaini Wraight for assistance with graphic design. Mention of trade names or commercial products in this publication is solely for the purpose of providing scientific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 36 TC 0 Z9 0 U1 14 U2 20 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD JUN PY 2016 VL 97 BP 31 EP 47 DI 10.1016/j.biocontrol.2016.02.016 PG 17 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DJ9CL UT WOS:000374511500004 ER PT J AU Brennan, EB AF Brennan, Eric B. TI Agronomy of strip intercropping broccoli with alyssum for biological control of aphids SO BIOLOGICAL CONTROL LA English DT Article DE Intercropping; Organic farming; Biological control of aphids; Broccoli; Alyssum; Vegetable production; Competition; Flowering; Beneficial insects; Hoverfly; Parasitoid ID HILARIS HEMIPTERA-PENTATOMIDAE; FLORAL RESOURCES; BREVICORYNE-BRASSICAE; HABITAT MANAGEMENT; WEED SUPPRESSION; NATURAL ENEMIES; LIVING MULCHES; CALIFORNIA; LETTUCE; GROWTH AB Organic broccoli growers in California typically control aphids by intercropping broccoli with strips of alyssum (Lobularia maritima (L.) Desv.) which attracts hoverflies (Diptera: Syrphidae) that are important predators of aphids. A three year study with transplanted organic broccoli in Salinas, California evaluated agronomic aspects of broccoli monoculture (B100) and broccoli-alyssum strip intercropping on beds in replacement intercropping treatments where alyssum transplants replaced 4 or 8% of the broccoli transplants, and an additive intercropping treatment (B100 + A100) where alyssum transplants were interspersed between broccoli without displacing it. The replacement patterns included alyssum planted on both lines of a bed (A100), beds with 50% broccoli and 50% alyssum transplants in different lines (B50A50D), and beds with 50% broccoli and 50% alyssum alternating in the same lines (B50A50S). To evaluate competition, shoot dry matter (DM) of alyssum and broccoli was measured at 36-43 days (harvest 1) and 59-66 days (harvest 2) after transplanting, and alyssum flowering was assessed at both harvests. The treatments performed consistently across years. The number of flowering alyssum shoots was highly correlated with alyssum DM. Per alyssum transplant, alyssum DM was highest in A100 and B50A50D at harvest 1, and by harvest 2 (3-7 days before broccoli maturity) was in order of A100 > B50A50D > B50A50S = B100 + A100. Broccoli was much more competitive than alyssum and by harvest 2 produced larger broccoli shoots per transplant in B50A50S (122 g) and B5S50D (96 g) than the more ideally sized shoots (73 g) in B100 and B100 + A100. The A100 pattern may be the most efficient replacement intercropping strategy to provide hoverflies and parasitoids with floral resources through the whole season, however, additive intercropping may also be useful to augment floral resources early in the season without displacing broccoli. These results can help growers reduce the cost of alyssum intercropping in high density broccoli systems (>100,000 transplants per ha). The practical management implications and future research needs to further improve the efficiency of these systems are discussed. Published by Elsevier Inc. C1 [Brennan, Eric B.] ARS, USDA, US Agr Res Stn, 1636 East Alisal St, Salinas, CA 93905 USA. RP Brennan, EB (reprint author), ARS, USDA, US Agr Res Stn, 1636 East Alisal St, Salinas, CA 93905 USA. EM Eric.Brennan@ars.usda.gov NR 48 TC 0 Z9 0 U1 29 U2 63 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD JUN PY 2016 VL 97 BP 109 EP 119 DI 10.1016/j.biocontrol.2016.02.015 PG 11 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DJ9CL UT WOS:000374511500012 ER PT J AU Morrison, WR Mathews, CR Leskey, TC AF Morrison, William R., III Mathews, Clarissa R. Leskey, Tracy C. TI Frequency, efficiency, and physical characteristics of predation by generalist predators of brown marmorated stink bug (Hemiptera: Pentatomidae) eggs SO BIOLOGICAL CONTROL LA English DT Article DE Biological control; Halyomorpha halys; Integrated pest management; Handling behavior; Sentinel egg masses ID HALYOMORPHA-HALYS HEMIPTERA; BIOLOGICAL-CONTROL; HETEROPTERA-PENTATOMIDAE; MANAGEMENT; BEHAVIOR; SPIDERS; PARASITISM; FIELDS; PEST; ORCHARDS AB The native generalist predator community of Halyomorpha halys, an invasive species in the United States and Europe, has not been well defined to date. The aims of the current study were to determine whether generalist predators that are commonly found in mid-Atlantic orchards and vegetable crops are capable of feeding on H. halys eggs and, if so, to systematically characterize the appearance of feeding damage in order to link it to sentinel egg mass surveys. Over 25 field-collected and commercially available arthropod predator taxa, including adults and immatures, were evaluated as potential predators of H. halys eggs in laboratory trials, and a photographic library of egg mass damage was developed. In addition, over 400 sentinel egg masses were deployed in tree fruit and vegetable crops, and direct observations were made of predator taxa in situ. We found that the most frequent and efficient predators of H. halys eggs were katydids and ground beetles, and to a lesser extent earwigs, jumping spiders, and crickets. Egg damage for each taxon studied was ascribed to one of four egg damage syndromes: complete chewing, incomplete chewing, stylet sucking, and punctured sucking. The first two are caused by predators with chewing mouthparts and are differentiated by the presence of irregular chorion debris left behind in the case of incomplete chewing compared with nothing left behind or an entire segment of the egg mass completely removed for complete chewing. The latter two are caused by predators with piercing/sucking mouthparts or chelicerae and are separable by the presence of a feeding sheath for stylet sucking compared to a simple hole or slit in otherwise hollowed eggs for punctured sucking via chelicerae. Complete and incomplete chewing were the two feeding syndromes most frequently documented in the field and with the greatest number of eggs consumed per egg mass. Taxa evaluated in laboratory trials were reliably found in tree fruit and vegetable crops. Overall, our work contributes to the identification of key egg mass predators of H. halys in specialty crop agroecosytems. Published by Elsevier Inc. C1 [Morrison, William R., III; Leskey, Tracy C.] ARS, USDA, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. [Mathews, Clarissa R.] Shepherd Univ, Inst Environm & Phys Sci, Shepherdstown, WV 25443 USA. RP Morrison, WR (reprint author), ARS, USDA, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM william.morrison@ars.usda.gov OI Morrison, William/0000-0002-1663-8741 FU USDA-NIFA Organic Research and Extension Initiative [2012-51300-20097]; USDA-NIFA SCRI CAP [2011-51181-30937] FX We thank Thomas Dorsey and the Beneficial Insect Laboratory (Trenton, NJ) for providing H. halys egg masses. We also thank McKenzie Allen, Morgan Douglas, Hector Nevarro-Guitz, Brittany Poling, Cecilia Sanchez and Sam Woullard for their excellent laboratory and field support. This research was funded, in part, by the USDA-NIFA Organic Research and Extension Initiative (Grant # 2012-51300-20097) and a USDA-NIFA SCRI CAP (Grant # 2011-51181-30937). NR 49 TC 7 Z9 7 U1 9 U2 30 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD JUN PY 2016 VL 97 BP 120 EP 130 DI 10.1016/j.biocontrol.2016.03.008 PG 11 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DJ9CL UT WOS:000374511500013 ER PT J AU Blackburn, D Shapiro-Ilan, DI Adams, BJ AF Blackburn, Dana Shapiro-Ilan, David I. Adams, Byron J. TI Biological control and nutrition: Food for thought SO BIOLOGICAL CONTROL LA English DT Review DE Biological control; Nutrition; Entomopathogens; Parasitoid; Predator ID THURINGIENSIS-BASED BIOPESTICIDES; ENTOMOPATHOGENIC NEMATODE PRODUCTION; BACTERIUM PHOTORHABDUS-LUMINESCENS; NUCLEAR POLYHEDROSIS-VIRUS; INTEGRATED PEST-MANAGEMENT; DELTA-ENDOTOXIN PRODUCTION; FUNGUS BEAUVERIA-BASSIANA; WASTE-WATER SLUDGE; YEAST CANDIDA-SAKE; HETERORHABDITIS-BACTERIOPHORA AB Biological control agents including a wide range of organisms such as predators, parasitoids, and entomopathogens (bacteria, fungi, nematodes, and viruses) are frequently used to control insect pests. Despite commercial availability of these biocontrol agents their widespread use is limited due to biological and economic difficulties. Efficient mass-production relies heavily on the environment in which the agent is grown. Nutrition can play a significant role in important biocontrol traits such as colonization and survival, tolerance to environmental stress, reproduction, and longevity. Therefore, to increase biocontrol potential nutritional aspects should be considered prior to commercial production. This review aims to explore the role nutrition plays in the production and efficacy of biocontrol agents by summarizing the effect nutrition has on important biocontrol traits, specifically traits in organisms that target insect pests including predators, parasitoids, and microbial agents. (C) 2016 Elsevier Inc. All rights reserved. C1 [Blackburn, Dana; Adams, Byron J.] Brigham Young Univ, Dept Biol, 4102 Life Sci Bldg, Provo, UT 84602 USA. [Shapiro-Ilan, David I.] ARS, USDA, SE Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. [Blackburn, Dana] Texas A&M Univ, Dept Vet Pathobiol, 4467 TAMU, College Stn, TX 77843 USA. RP Blackburn, D (reprint author), Brigham Young Univ, Dept Biol, 4102 Life Sci Bldg, Provo, UT 84602 USA.; Blackburn, D (reprint author), Texas A&M Univ, Dept Vet Pathobiol, 4467 TAMU, College Stn, TX 77843 USA. EM blackbud@byu.edu; David.Shapiro@ars.usda.gov; byron_adams@byu.edu RI Adams, Byron/C-3808-2009; OI Adams, Byron/0000-0002-7815-3352; Blackburn, Dana/0000-0001-5153-3366 NR 125 TC 0 Z9 1 U1 32 U2 71 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD JUN PY 2016 VL 97 BP 131 EP 138 DI 10.1016/j.biocontrol.2016.03.007 PG 8 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DJ9CL UT WOS:000374511500014 ER PT J AU Galbraith, ML Vorachek, WR Estill, CT Whanger, PD Bobe, G Davis, TZ Hall, JA AF Galbraith, M. L. Vorachek, W. R. Estill, C. T. Whanger, P. D. Bobe, G. Davis, T. Z. Hall, J. A. TI Rumen Microorganisms Decrease Bioavailability of Inorganic Selenium Supplements SO BIOLOGICAL TRACE ELEMENT RESEARCH LA English DT Article DE Rumen microorganisms; Ruminant; Selenium bioavailability; Selenium supplements; Sheep ID WHITE MUSCLE DISEASE; COMPARATIVE METABOLISM; DAIRY-COWS; SHEEP; CATTLE; LAMBS; 75SE-SELENOMETHIONINE; 75SE-SELENITE; DEFICIENCY; SELENATE AB Despite the availability of selenium (Se)-enriched trace mineral supplements, we have observed low Se status in cattle and sheep offered traditional inorganic Se supplements. Reasons for this may include inadequate intake or low bioavailability of inorganic Se sources. The objective of this study was to determine whether rumen microorganisms (RMO) alter the bioavailability of Se sources commonly used in Se supplements. Rumen microorganisms were isolated from ewes (n = 4) and incubated ex vivo with no Se (control), with inorganic Na selenite or Na selenate, or with organic selenomethionine (SeMet). Total Se incorporated into RMO and the amount of elemental Se formed were determined under equivalent conditions. Incorporation of Se from Na selenite, Na selenate, or SeMet into RMO was measured as fold change compared with control (no added Se). Incorporation of Se into microbial mass was greater for SeMet (13.2-fold greater than no-Se control) compared with inorganic Se supplements (P = 0.02); no differences were observed between inorganic Na selenate (3.3-fold greater than no-Se control) and Na selenite (3.5-fold greater than no-Se control; P = 0.97). Formation of non-bioavailable, elemental Se was less for RMO incubated with SeMet compared with inorganic Se sources (P = 0.01); no differences were observed between Na selenate and Na selenite (P = 0.09). The clinical importance of these results is that the oral bioavailability of organic SeMet should be greater compared with inorganic Se sources because of greater RMO incorporation of Se and decreased formation of elemental Se by RMO. C1 [Galbraith, M. L.; Vorachek, W. R.; Hall, J. A.] Oregon State Univ, Coll Vet Med, Dept Biomed Sci, Dryden Hall 206, Corvallis, OR 97331 USA. [Estill, C. T.] Oregon State Univ, Coll Vet Med, Dept Clin Sci, Corvallis, OR 97331 USA. [Estill, C. T.; Bobe, G.] Oregon State Univ, Coll Agr Sci, Dept Anim & Rangeland Sci, Corvallis, OR 97331 USA. [Whanger, P. D.] Oregon State Univ, Coll Agr Sci, Dept Environm & Mol Toxicol, Corvallis, OR 97331 USA. [Bobe, G.] Oregon State Univ, Linus Pauling Inst, Corvallis, OR 97331 USA. [Davis, T. Z.] USDA ARS, Poisonous Plant Res Lab, 1150 E 1400 N, Logan, UT 84341 USA. [Hall, J. A.] Oregon State Univ, Agr Res Fdn, Corvallis, OR 97331 USA. RP Hall, JA (reprint author), Oregon State Univ, Coll Vet Med, Dept Biomed Sci, Dryden Hall 206, Corvallis, OR 97331 USA.; Hall, JA (reprint author), Oregon State Univ, Agr Res Fdn, Corvallis, OR 97331 USA. EM Jean.Hall@oregonstate.edu NR 38 TC 0 Z9 0 U1 13 U2 17 PU HUMANA PRESS INC PI TOTOWA PA 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA SN 0163-4984 EI 1559-0720 J9 BIOL TRACE ELEM RES JI Biol. Trace Elem. Res. PD JUN PY 2016 VL 171 IS 2 BP 338 EP 343 DI 10.1007/s12011-015-0560-8 PG 6 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA DL6ZC UT WOS:000375787600014 PM 26537117 ER PT J AU Wang, XY Cao, LM Yang, ZQ Duan, JJ Gould, JR Bauer, LS AF Wang, Xiao-Yi Cao, Liang-Ming Yang, Zhong-Qi Duan, Jian J. Gould, Juli R. Bauer, Leah S. TI Natural enemies of emerald ash borer (Coleoptera: Buprestidae) in northeast China, with notes on two species of parasitic Coleoptera SO CANADIAN ENTOMOLOGIST LA English DT Article ID AGRILUS-PLANIPENNIS COLEOPTERA; RUSSIAN FAR-EAST; HYMENOPTERA-BRACONIDAE; SEASONAL ABUNDANCE; BIOLOGICAL-CONTROL; BIOTIC FACTORS; ENCYRTIDAE; AMERICA; EULOPHIDAE; MICHIGAN AB To investigate natural enemies of emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), in northeastern China, we conducted field surveys of ash (Fraxinus Linnaeus (Oleaceae)) trees in semi-natural forests and plantations at variable EAB densities from 2008 to 2013. Our surveys revealed a complex of natural enemies including eight hymenopteran parasitoids and two apparently parasitic Coleoptera, woodpeckers, and several undetermined mortality factors. Parasitoid complex abundance and its contribution to EAB mortality varied with the time of year, type of ash stands, and geographic regions. The egg parasitoid Oobius agrili Zhang and Huang (Hymenoptera: Encyrtidae) and the larval parasitoid Tetrastichus planipennisi Yang (Hymenoptera: Eulophidae) were frequently observed in Jilin, Liaoning, and Heilongjiang provinces and in Beijing, but not in Tianjin. Spathius agrili Yang (Hymenoptera: Braconidae), however, was more prevalent near Beijing and further south in Tianjin. Larvae of two species of apparently parasitic beetle, Tenerus Laporte (Coleoptera: Cleridae) species and Xenoglena quadrisignata Mannerheim (Coleoptera: Trogossitidae), were also recovered attacking overwintering EAB in Liaoning Province, with Tenerus species being a dominant mortality agent (similar to 13%). Our findings support the need to consider the geographic origin of insect natural enemies for EAB biocontrol, as well as an expanded foreign exploration for EAB natural enemies throughout its native range in Asia. C1 [Wang, Xiao-Yi; Cao, Liang-Ming; Yang, Zhong-Qi] Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, State Forestry Adm, Key Lab Forest Protect, Beijing 100091, Peoples R China. [Duan, Jian J.] ARS, USDA, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. [Gould, Juli R.] US Anim & Plant Hlth Inspect Serv, USDA, Plant Protect & Quarantine, Ctr Plant Hlth Sci & Technol, Buzzards Bay, MA 02542 USA. [Bauer, Leah S.] US Forest Serv, USDA, No Res Stn, Lansing, MI 48910 USA. RP Wang, XY (reprint author), Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, State Forestry Adm, Key Lab Forest Protect, Beijing 100091, Peoples R China. EM xywang@caf.ac.cn FU United States Department of Agriculture, Animal and Plant Health Inspection Service, Plant Protection and Quarantine Program; United States Department of Agriculture, Forest Service International Program; United States Department of Agriculture, Agricultural Research Service; China Scholarship Fund [201203270012] FX The authors thank Mr. Xian-Wen Zhang and Mr. Yun-Cheng Liu of Saima Forest Station of Fengcheng City, Liaoning Province for assistance during our field investigation. They thank Professor Shou-Hui Sun and his two master students (College of Forestry, Shenyang Agricultural University) for helping of partial dissection work in Nursery of Shenyang Agricultural University. They thank Dr. Gan-Yan Yang (Institute of Zoology, Chinese Academy of Sciences) for identification of Tenerus species, and Professor Mao-Ling Sheng (General Station of Forest Pest Management, State Forestry Administration, Shenyang, Liaoning, China) for identification of Xorides species. The authors thank Tom Luther (United States Department of Agriculture, Forest Service) for creating the map. This work was partially supported by United States Department of Agriculture, Animal and Plant Health Inspection Service, Plant Protection and Quarantine Program, United States Department of Agriculture, Forest Service International Program, and United States Department of Agriculture, Agricultural Research Service. Xiao-Yi Wang is grateful to the China Scholarship Fund (201203270012) for supporting his visit to United States Department of Agriculture, Agricultural Research Service, Beneficial Insects Introduction Research Unit. They appreciate comments on an early draft of this manuscript by Dr. David E. Jennings (University of Maryland), and they thank two anonymous reviewers for their valuable suggestions. NR 39 TC 1 Z9 1 U1 10 U2 11 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X EI 1918-3240 J9 CAN ENTOMOL JI Can. Entomol. PD JUN PY 2016 VL 148 IS 3 BP 329 EP 342 DI 10.4039/tce.2015.57 PG 14 WC Entomology SC Entomology GA DL4JJ UT WOS:000375600300007 ER PT J AU Neel, JPS Felton, EED Singh, S Sexstone, AJ Belesky, DP AF Neel, J. P. S. Felton, E. E. D. Singh, S. Sexstone, A. J. Belesky, D. P. TI Open pasture, silvopasture and sward herbage maturity effects on nutritive value and fermentation characteristics of cool-season pasture SO GRASS AND FORAGE SCIENCE LA English DT Article DE fermentation; forage quality; pastoral systems; chemical composition; nutritive value; stage of maturity; silvopasture ID MICROBIAL-GROWTH; RUMEN; POPULATION; QUALITY; SYSTEM; SHADE; TIME; DIET AB In Appalachian USA, silvopasture offers promise of increased farm productivity. A synchronized, temporal understanding of open pasture (OP) and silvopasture (SP) nutritive characteristics is essential for grazing system development. We examined pasture-type nutritive-value relationships when herbage was harvested based on morphological maturity rather than calendar date. Neutral detergent fibre and acid detergent fibre (ADF and NDF) content were greater in silvopasture, while organic matter (OM) was lower (P<005). Digestibility of SP herbage dry matter (DM) and OM tended (P=010) to be lower (418 vs. 471 and 437 vs. 491gkg(-1) respectively). Neutral detergent fibre digestibility was greater (P<005) for OP than SP forage (538 vs. 480gkg(-1)), and ADF tended to be greater (P=010; 551 vs. 501). Open-pasture forage fermentation effluent exhibited slightly higher microbial richness and Shannon diversity than SP. However, overall community composition of both bacteria and archaea did not differ between pasture types or sampling times. Pasture types show proximate analyses differences generally favourable to OP, although both have similar overall nutritive value. In addition, the SP sward exhibited a 4- to 6-days delay in reaching equivalent maturity. Conversion of farm woodlots to SP would increase overall herbage production and improve pasture management flexibility. C1 [Neel, J. P. S.; Belesky, D. P.] USDA ARS, Appalachian Farming Syst Res Ctr, Beaver, WV USA. [Felton, E. E. D.; Singh, S.; Sexstone, A. J.; Belesky, D. P.] W Virginia Univ, Morgantown, WV 26505 USA. RP Neel, JPS (reprint author), USDA ARS, Grazinglands Res Lab, 7207 W Cheyenne St, El Reno, OK 73036 USA. EM Jim.Neel@ars.usda.gov NR 32 TC 0 Z9 0 U1 3 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0142-5242 EI 1365-2494 J9 GRASS FORAGE SCI JI Grass Forage Sci. PD JUN PY 2016 VL 71 IS 2 BP 259 EP 269 DI 10.1111/gfs.12172 PG 11 WC Agronomy SC Agriculture GA DL9ZK UT WOS:000376001900008 ER PT J AU Decker, JE Taylor, JF Kantanen, J Millbrooke, A Schnabel, RD Alexander, LJ MacNeil, MD AF Decker, J. E. Taylor, J. F. Kantanen, J. Millbrooke, A. Schnabel, R. D. Alexander, L. J. MacNeil, M. D. TI Origins of cattle on Chirikof Island, Alaska, elucidated from genome-wide SNP genotypes SO HEREDITY LA English DT Article ID POPULATIONS; SELECTION; ANGUS AB Feral livestock may harbor genetic variation of commercial, scientific, historical or esthetic value. The origins and uniqueness of feral cattle on Chirikof Island, Alaska, are uncertain. The island is now part of the Alaska Maritime Wildlife Refuge and Federal wildlife managers want grazing to cease, presumably leading to demise of the cattle. Here we characterize the cattle of Chirikof Island relative to extant breeds and discern their origins. Our analyses support the inference that Yakut cattle from Russia arrived first on Chirikof Island, then similar to 120 years ago the first European taurine cattle were introduced to the island, and finally a large wave of Hereford cattle were introduced on average 40 years ago. In addition, this mixture of European and East-Asian cattle is unique compared with other North American breeds and we find evidence that natural selection in the relatively harsh environment of Chirikof Island has further impacted their genetic architecture. These results provide an objective basis for decisions regarding conservation of the Chirikof Island cattle. C1 [Decker, J. E.] Univ Missouri, Anim Sci Res Ctr S132B, 920 East Campus Dr, Columbia, MO 65211 USA. [Taylor, J. F.] Univ Missouri, Anim Sci Res Ctr S135A, Columbia, MO USA. [Kantanen, J.] Nat Resources Inst Finland Luke, Green Technol, Jokioinen, Finland. [Kantanen, J.] Univ Eastern Finland, Dept Biol, Kuopio, Finland. [Millbrooke, A.] Embry Riddle Aeronaut Univ, Daytona Beach, FL USA. [Schnabel, R. D.] Univ Missouri, Anim Sci Res Ctr 162, Columbia, MO USA. [Alexander, L. J.; MacNeil, M. D.] USDA ARS, Miles City, MT USA. [MacNeil, M. D.] Delta G, 145 Ice Cave Rd, Miles City, MT 59301 USA. RP Decker, JE (reprint author), Univ Missouri, Anim Sci Res Ctr S132B, 920 East Campus Dr, Columbia, MO 65211 USA.; MacNeil, MD (reprint author), Delta G, 145 Ice Cave Rd, Miles City, MT 59301 USA. EM deckerje@missouri.edu; macneil.deltag@gmail.com FU National Research Initiative competitive grant from the USDA National Institute of Food and Agriculture [2011-68004-30214, 2011-68004-30367, 2013-68004-20364, 2015-67015-23183]; Hatch Project fund [MO-HAAS0027]; USDA [NSRP-8 MO-MSAS0014]; Academy of Finland [286040] FX JFT was supported by National Research Initiative competitive grant numbers 2011-68004-30214, 2011-68004-30367, 2013-68004-20364 and 2015-67015-23183 from the USDA National Institute of Food and Agriculture. JED was supported by Hatch Project MO-HAAS0027 and USDA NSRP-8 MO-MSAS0014 funds. Zoya Ivanova, Ruslan Popov, Innokentyi Ammosov and Mikhail Ozerov are acknowledged for collaboration in sampling of Yakut and Kalmyk breeds. JK was supported by the Academy of Finland grant number 286040. NR 27 TC 0 Z9 0 U1 3 U2 8 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0018-067X EI 1365-2540 J9 HEREDITY JI Heredity PD JUN PY 2016 VL 116 IS 6 BP 502 EP 505 DI 10.1038/hdy.2016.7 PG 4 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA DL5TP UT WOS:000375700100003 PM 26860198 ER PT J AU Kandala, CV Holser, R Settaluri, V Mani, S Puppala, N AF Kandala, Chari V. Holser, Ronald Settaluri, Vijayasaradhi Mani, Sudhagar Puppala, Naveen TI Capacitance Sensing of Moisture Content in Fuel Wood Chips SO IEEE SENSORS JOURNAL LA English DT Article DE Biofuel; moisture content; dielectric; capacitance; radio frequencies; phase angle ID SENSOR AB Moisture content of wood chips is an important factor to be known in their utilization as a biomass material. Several moisture measuring instruments are available in the market, but for most of these instruments, some sort of sample preparation is needed that involves sizing, grinding, and weighing. The samples in this process are usually destroyed, and the measurement involves considerable time and labor. The standard methods of oven drying and Karl Fisher also fall in the destructive and laborious category. In this paper, estimation of moisture content of hardwood chips, from the measurement of certain electrical properties of a parallel-plate capacitor, holding the samples of these materials between them at two radio frequencies, is presented. The wood chip samples tested were in the moisture range of 3%-50%. The standard error of prediction was 2.0, and the prediction accuracies were better for the samples with moisture contents below 25%. The construction and performance of a prototype moisture sensing instrument working on these principles is briefly described. This method being nondestructive and rapid would be useful in the biofuel industry. C1 [Kandala, Chari V.] USDA, Natl Peanut Res Lab, Dawson, GA 39842 USA. [Holser, Ronald] USDA, Athens, GA 30605 USA. [Settaluri, Vijayasaradhi] KL Univ, Dept Biotechnol, Guntur 522502, India. [Settaluri, Vijayasaradhi] Higher Coll Technol, Dept Appl Sci, Muscat, Oman. [Mani, Sudhagar] Univ Georgia, Fac Engn, Athens, GA 30602 USA. [Puppala, Naveen] New Mexico State Univ, Clovis, NM 88101 USA. RP Kandala, CV (reprint author), USDA, Natl Peanut Res Lab, Dawson, GA 39842 USA.; Settaluri, V (reprint author), Higher Coll Technol, Dept Appl Sci, Muscat, Oman.; Mani, S (reprint author), Univ Georgia, Fac Engn, Athens, GA 30602 USA.; Puppala, N (reprint author), New Mexico State Univ, Clovis, NM 88101 USA. EM chari.kandala@ars.usda.gov; bionanocat@gmail.com; spsree@yahoo.com; smani@engr.uga.edu; npuppala@ad.nmsu.edu NR 16 TC 0 Z9 0 U1 2 U2 8 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1530-437X EI 1558-1748 J9 IEEE SENS J JI IEEE Sens. J. PD JUN 1 PY 2016 VL 16 IS 11 SI SI BP 4509 EP 4514 DI 10.1109/JSEN.2016.2541081 PG 6 WC Engineering, Electrical & Electronic; Instruments & Instrumentation; Physics, Applied SC Engineering; Instruments & Instrumentation; Physics GA DL3WH UT WOS:000375563700064 ER PT J AU Rosenthal, BM Dunams-Morel, D Ostoros, G Molnar, K AF Rosenthal, Benjamin M. Dunams-Morel, Detiger Ostoros, Gyorgyi Molnar, Kalman TI Coccidian parasites of fish encompass profound phylogenetic diversity and gave rise to each of the major parasitic groups in terrestrial vertebrates SO INFECTION GENETICS AND EVOLUTION LA English DT Article DE Coccidia; Evolution; Parasitism; Origins; Vertebrates; Phylogeny ID ASIAN SEA-BASS; APICOMPLEXA EIMERIIDAE; MAXIMUM-LIKELIHOOD; GOUSSIA LABBE; EIMERIORINA; VIETNAM AB Fish are the oldest and most diverse group of vertebrates; it therefore stands to reason that fish may have been the original hosts for many types of extant vertebrate parasites. Here, we sought to determine whether coccidian parasites of fish are especially diverse. We therefore sampled such parasites from thirty-nine species of fish and tested phylogenetic hypotheses concerning their relationships, using 18S rDNA. We found compelling phylogenetic support for distinctions among at least four lineages of piscine parasites presently ascribed to the genus Goussia. Some, but not all parasites attributed to Eimeria were confirmed as such. Major taxonomic revisions are likely justified for these parasites of fish, which appear to have given rise to each of the major lineages of coccidian parasites that subsequently proliferated in terrestrial vertebrates, including those such as Toxoplasma gondii that form tissue cysts in intermediate hosts. Published by Elsevier B.V. C1 [Rosenthal, Benjamin M.; Dunams-Morel, Detiger] ARS, Beltsville Agr Res Ctr, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. [Ostoros, Gyorgyi; Molnar, Kalman] Hungarian Acad Sci, Vet Med Res Inst, Agr Res Ctr, Hungaria Krt 21, H-1143 Budapest, Hungary. RP Rosenthal, BM (reprint author), ARS, Beltsville Agr Res Ctr, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Benjamin.rosenthal@ars.usda.gov OI Rosenthal, Benjamin/0000-0002-0224-3773 FU Hungarian Scientific Research Fund (OTKA) [K100132]; Organization for Economic Cooperation and Development (OECD) Biological Resource Management for Sustainable Agricultural Systems Travel Fellowship; USDA-ARS [410000-8042-016-00D] FX This work was supported by the Hungarian Scientific Research Fund (OTKA, project number K100132), an Organization for Economic Cooperation and Development (OECD) Biological Resource Management for Sustainable Agricultural Systems Travel Fellowship, and USDA-ARS Project 410000-8042-016-00D. NR 40 TC 1 Z9 1 U1 3 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1567-1348 EI 1567-7257 J9 INFECT GENET EVOL JI Infect. Genet. Evol. PD JUN PY 2016 VL 40 BP 219 EP 227 DI 10.1016/j.meegid.2016.02.018 PG 9 WC Infectious Diseases SC Infectious Diseases GA DK2TT UT WOS:000374768000031 PM 26911642 ER PT J AU Nagoshi, RN Meagher, RL AF Nagoshi, R. N. Meagher, R. L. TI Using intron sequence comparisons in the triose-phosphate isomerase gene to study the divergence of the fall armyworm host strains SO INSECT MOLECULAR BIOLOGY LA English DT Article DE Spodoptera frugiperda; haplotype; sympatric speciation ID SPODOPTERA-FRUGIPERDA LEPIDOPTERA; FRAGMENT-LENGTH-POLYMORPHISMS; SYMPATRIC SPECIATION; POPULATION-STRUCTURE; DNA POLYMORPHISM; PUERTO-RICO; BT MAIZE; NOCTUIDAE; IDENTIFICATION; RESISTANCE AB The noctuid moth Spodoptera frugiperda (the fall armyworm) is endemic to the Western Hemisphere and appears to be undergoing sympatric speciation to produce two subpopulations that differ in their choice of host plants. The 'rice strain' and 'corn strain' are morphologically indistinguishable, requiring the use of genetic markers for identification. Because fall armyworm is a major pest of corn and several other agricultural crops, characterizing the strains has important economic consequences. In this study, comparisons were made of the intron sequences from the triose-phosphate isomerase (Tpi) gene isolated from 85 fall armyworm specimens collected from two host plants. Sixteen new strainspecific haplotypes based on intron polymorphisms are described that can facilitate the characterization of fall armyworm populations associated with different host plants. Comparisons of genetic diversity within and between the strains provides evidence that the corn strain is undergoing active selection and supports the proposal of directional interstrain mating occurring in the wild. Comparisons of the polymorphisms indicate that each intron undergoes different patterns of mutation that in some cases corresponds to host plant preferences. The results confirm that intron sequence comparisons are an effective approach to study fall armyworm population genetics. C1 [Nagoshi, R. N.; Meagher, R. L.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. RP Nagoshi, RN (reprint author), USDA ARS, CMAVE, Ctr Med Agr & Vet Entomol, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. EM rodney.nagoshi@ars.usda.gov FU National Institute of Food and Agriculture, US. Department of Agriculture [2011-67003-30209] FX We thank Jean Thomas and Mirian Hay-Roe at the United States Department of Agriculture Agricultural Research Service (USDA-ARS) for excellent technical support and contributions to this research, and Steven Valles (USDA-ARS) for important suggestions for the manuscript. We recognize the financial support of the National Institute of Food and Agriculture, US. Department of Agriculture, under Agreement No. 2011-67003-30209. The use of trade, firm or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the USDA or the ARS of any product or service to the exclusion of others that may be suitable. NR 48 TC 1 Z9 1 U1 2 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1075 EI 1365-2583 J9 INSECT MOL BIOL JI Insect Mol. Biol. PD JUN PY 2016 VL 25 IS 3 BP 324 EP 337 DI 10.1111/imb.12223 PG 14 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA DL5IE UT WOS:000375669800012 PM 26991678 ER PT J AU Dong, HT Senapin, S LaFrentz, B Rodkhum, C AF Dong, H. T. Senapin, S. LaFrentz, B. Rodkhum, C. TI Virulence assay of rhizoid and non-rhizoid morphotypes ofFlavobacterium columnare in red tilapia, Oreochromis sp., fry SO JOURNAL OF FISH DISEASES LA English DT Article DE colony morphotypes; Flavobacterium columnare; tilapia; virulence ID CATFISH ICTALURUS-PUNCTATUS; FLAVOBACTERIUM-COLUMNARE; FLEXIBACTER-COLUMNARIS; FISH PATHOGEN; INFECTION; ADHESION; STRAINS; DISEASE AB Numerous isolates of Flavobacterium columnare were previously recovered from red tilapia, Oreochromis sp., exhibiting columnaris-like disease in Thai farms, and the phenotypic and genetic characteristics were described. The objective of this study was to determine the virulence of two morphotypes (rhizoid and non-rhizoid colonies) of F.columnare and to determine their ability to adhere to and persist in red tilapia fry. The results showed that the typical rhizoid isolate (CUVET1214) was a highly virulent isolate and caused 100% mortality within 24h following bath challenge of red tilapia with three different doses. The non-rhizoid isolate (CUVET1201) was avirulent to red tilapia fry. Both morphotypes adhered to and persisted in tilapia similarly at 0.5 and 6h post-challenge as determined by whole fish bacterial loads. At 24 and 48h post-challenge, fry challenged with the rhizoid morphotype exhibited significantly higher bacterial loads than the non-rhizoid morphotype. The results suggested that an inability of the non-rhizoid morphotype to persist in tilapia fry may explain lack of virulence. C1 [Dong, H. T.; Rodkhum, C.] Chulalongkorn Univ, Fac Vet Sci, Dept Vet Microbiol, Bangkok 10330, Thailand. [Senapin, S.] Natl Sci & Technol Dev Agcy, Natl Ctr Genet Engn & Biotechnol, Pathum Thani, Thailand. [Senapin, S.] Mahidol Univ, Ctr Excellence Shrimp Mol Biol & Biotechnol, Bangkok 10700, Thailand. [LaFrentz, B.] USDA ARS, Aquat Anim Hlth Res Unit, Auburn, AL USA. RP Rodkhum, C (reprint author), Chulalongkorn Univ, Fac Vet Sci, Dept Vet Microbiol, Bangkok 10330, Thailand. EM channarong_r@yahoo.com RI Dong, Ha/N-4300-2016; OI Dong, Ha/0000-0002-7190-757X; Senapin, Saengchan/0000-0002-5599-4343 FU Chulalongkorn University FX This research was supported by the 100th year anniversary of Chulalongkorn University fund for doctoral scholarship and the 90th anniversary of Chulalongkorn University fund (Ratchadaphisek-somphot Endowment Fund). The authors thank the Veterinary Medical Aquatic Research Center (VMARC), Chulalongkorn University for providing some equipment for the experimental challenge and Dr Pattanapon Kayansamruaj for statistical advices. NR 23 TC 0 Z9 0 U1 2 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7775 EI 1365-2761 J9 J FISH DIS JI J. Fish Dis. PD JUN PY 2016 VL 39 IS 6 BP 649 EP 655 DI 10.1111/jfd.12385 PG 7 WC Fisheries; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Marine & Freshwater Biology; Veterinary Sciences GA DL6XO UT WOS:000375783600002 PM 25953003 ER PT J AU Snyder, D Cernicchiaro, N Allan, SA Cohnstaedt, LW AF Snyder, D. Cernicchiaro, N. Allan, S. A. Cohnstaedt, L. W. TI Insecticidal sugar baits for adult biting midges SO MEDICAL AND VETERINARY ENTOMOLOGY LA English DT Article DE Culicoides; bait station oral toxicity; insecticide; midge control; toxic sugar bait ID SOUTHERN-CALIFORNIA; DIFFERENT MODES; FLIES DIPTERA; SUSCEPTIBILITY; SPINOSAD; MOSQUITOS; COCCINELLIDAE; THIAMETHOXAM; COLEOPTERA; BLOSSOMS AB The mixing of an insecticide with sugar solution creates an oral toxin or insecticidal sugar bait (ISB) useful for reducing adult insect populations. The ability of ISBs to kill the biting midge Culicoides sonorensisWirth and Jones (Diptera: Ceratopogonidae), a vector of bluetongue virus, epizootic hemorrhagic disease and vesicular stomatitis viruses, was tested. The commercial insecticide formulations (percentage active ingredient) tested included bifenthrin, cyfluthrin, deltamethrin, permethrin, dinotefuran, imidacloprid, thiamethoxam and spinosad. Mortality rates were determined for various concentrations of commercial formulations (0.01, 0.05, 0.1, 1, 2 and 3%) and observed at 1, 4, 10 and 24h post-exposure to the ISB. In the first set of assays, laboratory-reared midges were fed sugar ad libitum and then exposed to insecticide-treated sugar solutions to measure mortality. The second assay assessed competitive feeding: midges were provided with a control sugar solution (10% sucrose) in one vial, and a sugar and insecticide solution in another. Pyrethroid treatments resulted in the greatest mortality in the first hour at the lowest concentrations and spinosad consumption resulted in the least mortality. Biting midges were not deterred from feeding on the 1% ISB solutions despite the presence of an insecticide-free alternative source of sugar. C1 [Snyder, D.] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Cernicchiaro, N.] Kansas State Univ, Dept Diagnost Med & Pathobiol, Manhattan, KS 66506 USA. [Allan, S. A.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. [Cohnstaedt, L. W.] USDA ARS, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. RP Cohnstaedt, LW (reprint author), USDA ARS, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM Lee.Cohnstaedt@ars.usda.gov NR 28 TC 0 Z9 0 U1 5 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0269-283X EI 1365-2915 J9 MED VET ENTOMOL JI Med. Vet. Entomol. PD JUN PY 2016 VL 30 IS 2 BP 209 EP 217 DI 10.1111/mve.12158 PG 9 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA DL8GC UT WOS:000375878200010 PM 26789534 ER PT J AU Kumar, NHV Nayduch, D AF Kumar, N. H. V. Nayduch, D. TI Dose-dependent fate of GFP-expressing Escherichia coli in the alimentary canal of adult house flies SO MEDICAL AND VETERINARY ENTOMOLOGY LA English DT Article DE Bacteria; dissemination; vector potential ID MUSCA-DOMESTICA DIPTERA; TEMPOROSPATIAL FATE; BACTERIA; INSECTS; O157-H7; TRANSMISSION; ENVIRONMENT; COMMUNITY; MUSCIDAE; O157H7 AB The adult house fly Musca domestica (L.) (Diptera: Muscidae) can disseminate bacteria from microbe-rich substrates to areas in which humans and domesticated animals reside. Because bacterial abundance fluctuates widely across substrates, flies encounter and ingest varying amounts of bacteria. This study investigated the dose-dependent survival of bacteria in house flies. Flies were fed four different doses' of green fluorescent protein (GFP)-expressing Escherichia coli (GFPE.coli) (very low, low, medium, high) and survival was determined at 1, 4, 10 and 22h post-ingestion by culture and epifluorescent microscopy. Over 22h, the decline in GFPE.coli was significant in all treatments (P<0.04) except the very low dose treatment (P=0.235). Change in survival (S) did not differ between flies fed low and very low doses of bacteria across all time-points, although S in both treatments differed from that in flies fed high and medium doses of bacteria at several time-points. At 4, 10 and 22h, GFPE.coli S significantly differed between medium and high dose-fed flies. A threshold dose, above which bacteria are detected and destroyed by house flies, may exist and is likely to be immune-mediated. Understanding dose-dependent bacterial survival in flies can help in predicting bacteria transmission potential. C1 [Kumar, N. H. V.] Georgia So Univ, Dept Biol, Statesboro, GA 30460 USA. [Nayduch, D.] USDA ARS, Arthropod Borne Anim Dis Res Unit, 1515 Coll Ave, Manhattan, KS 66502 USA. RP Nayduch, D (reprint author), USDA ARS, Arthropod Borne Anim Dis Res Unit, 1515 Coll Ave, Manhattan, KS 66502 USA. EM dana.nayduch@ars.usda.gov FU National Institutes of Health (Bethesda, MD, U.S.A.) [1R15AI084029-01] FX The authors thank Dr Brian Weiss, Laboratory of Epidemiology and Public Health, Yale University (New Haven, CT, U.S.A.) for the pGFPuv-kanamycin plasmid construct. This work was supported by an R15 Academic Research Enhancement Award (AREA) 1R15AI084029-01 from the National Institutes of Health (Bethesda, MD, U.S.A.) to DN. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 30 TC 3 Z9 3 U1 0 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0269-283X EI 1365-2915 J9 MED VET ENTOMOL JI Med. Vet. Entomol. PD JUN PY 2016 VL 30 IS 2 BP 218 EP 228 DI 10.1111/mve.12162 PG 11 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA DL8GC UT WOS:000375878200011 PM 26843509 ER PT J AU Ulrich, KR Kramer, M Feldlaufer, MF AF Ulrich, Kevin R. Kramer, Matthew Feldlaufer, Mark F. TI Ability of bed bug (Hemiptera: Cimicidae) defensive secretions (E)-2-hexenal and (E)-2-octenal to attract adults of the common bed bug Cimex lectularius SO PHYSIOLOGICAL ENTOMOLOGY LA English DT Article DE Aldehydes; attraction; Cimex lectularius; defensive secretions ID HETEROPTERA CIMICIDAE; AGGREGATION BEHAVIOR; ALARM PHEROMONE; RESPONSES; SEMIOCHEMICALS; IDENTIFICATION; ARRESTMENT; VOLATILES; SIGNALS; CANINES AB Accurate and timely surveillance of bed bug infestations is critical for the development of effective control strategies. Although the bed hug-produced volatiles (E)-2-hexenal and (E)-2-octenal are considered as defensive secretions, the present study demonstrates, using ETHOVISION (R) video-tracking software (Noldus Information Technology Inc., Leesburg, Virginia), that low amounts of these commercially-obtained aldehydes function as attractants, and high amounts function as local repellents, against the common bed bug Cimex lectularius L. In behavioural assays, both males and female C. lectularius are attracted to 0.04 mu g of an aldehyde blend (1:1) for up to 1 h after initial treatment of filter paper disks. Males differ from females in their response to higher amounts of aldehydes, with females and males exhibiting maximum local repellency at 40 mu g and 400 mu g, respectively. The results suggest that these bed bug secretions may be candidates for lures and monitors. C1 [Ulrich, Kevin R.; Feldlaufer, Mark F.] USDA ARS, Invas Insect Biocontrol & Behav Lab, Bldg 1040,Room 2, Beltsville, MD 20705 USA. [Kramer, Matthew] USDA ARS, Stat Grp, Beltsville, MD 20705 USA. RP Feldlaufer, MF (reprint author), USDA ARS, Invas Insect Biocontrol & Behav Lab, Bldg 1040,Room 2, Beltsville, MD 20705 USA. EM mark.feldlaufer@ars.usda.gov NR 38 TC 2 Z9 2 U1 9 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0307-6962 EI 1365-3032 J9 PHYSIOL ENTOMOL JI Physiol. Entomol. PD JUN PY 2016 VL 41 IS 2 BP 103 EP 110 DI 10.1111/phen.12129 PG 8 WC Entomology SC Entomology GA DL6QV UT WOS:000375764600003 ER PT J AU Badillo-Vargas, IE Baker, CA Turechek, WW Frantz, G Mellinger, HC Funderburk, JE Adkins, S AF Badillo-Vargas, Ismael E. Baker, Carlye A. Turechek, William W. Frantz, Galen Mellinger, H. Charles Funderburk, Joseph E. Adkins, Scott TI Genomic and Biological Characterization of Tomato necrotic streak virus, a Novel Subgroup 2 Ilarvirus Infecting Tomato in Florida SO PLANT DISEASE LA English DT Article ID PARIETARIA-MOTTLE-VIRUS; TRANSMITTED VIRUSES; SEED TRANSMISSION; THRIPS-TABACI; RT-PCR; TOBACCO; POLLEN; TREES; PLANT AB Genomic and biological characterization of Tomato necrotic streak virus (TomNSV), a recently described ilarvirus infecting tomato in Florida, was completed. The full genome sequence revealed that TomNSV is a novel subgroup 2 ilarvirus that is distinct from other previously reported tomato-infecting ilarviruses: Tobacco streak virus, Parietaria mottle virus, and Tomato necrotic spot virus included in subgroup 1. In a host range experiment, TomNSV infected members of the Solanaceae and Chenopodiaceae plant families but did not infect sunflower (Helianthus annuus L.) or green bean (Phaseolus vulgaris L.). In tomato plants, the virus moved downward to the roots from the initial point of infection and then upward from the roots to tissues of active growth such as fruit, flowers, and young leaves where symptoms were produced. Thus, young leaves, fruit, and flowers are ideal for sampling for TomNSV. The transmission rate by seed collected from infected tomato plants was determined to be 0.33%. Collectively, the results of these experiments indicated that TomNSV is the causal agent of the necrotic streak disease of tomato observed in Florida since 2013. C1 [Badillo-Vargas, Ismael E.; Funderburk, Joseph E.] Univ Florida, NFREC, Quincy, FL 32351 USA. [Badillo-Vargas, Ismael E.; Turechek, William W.; Adkins, Scott] USDA ARS, Ft Pierce, FL 34945 USA. [Baker, Carlye A.] Florida Dept Agr & Consumer Serv, Div Plant Ind, Gainesville, FL 32608 USA. [Mellinger, H. Charles] Glades Crop Care Inc, Jupiter, FL 33458 USA. RP Adkins, S (reprint author), USDA ARS, Ft Pierce, FL 34945 USA. EM scott.adkins@ars.usda.gov FU Florida Specialty Crop Block Grant [21721] FX We thank C. Vanderspool, R. Lewis, J. Skaryd, S. Hanson, and R. Noel for their excellent technical assistance; and numerous scouts and growers for help with sample collection. This research was supported, in part, by Florida Specialty Crop Block Grant 21721. NR 32 TC 0 Z9 0 U1 1 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2016 VL 100 IS 6 BP 1046 EP 1053 DI 10.1094/PDIS-12-15-1437-RE PG 8 WC Plant Sciences SC Plant Sciences GA DL4UV UT WOS:000375634200002 ER PT J AU Saito, S Michailides, TJ Xiao, CL AF Saito, S. Michailides, T. J. Xiao, C. L. TI Mucor Rot-An Emerging Postharvest Disease of Mandarin Fruit Caused by Mucor piriformis and other Mucor spp. in California SO PLANT DISEASE LA English DT Article ID GREEN MOLD; CITRUS-FRUIT; STONE FRUITS; SOUR ROT; PENICILLIUM; SURVIVAL; ORCHARDS AB In recent years, an emerging, undescribed postharvest disease was observed on mandarin fruit after extended storage in California. We collected decayed mandarin fruit from three citrus packinghouses in the Central Valley of California in 2015 and identified this disease as Mucor rot caused by Mucor spp. Mucor rot occurred in 11 of the 15 grower lots sampled, and the percentage of Mucor rot in the total decayed fruit varied among affected grower lots, ranging from 3.3 to 93.1% with an average of 49.2%. In total, 197 isolates of Mucor spp. were obtained from decayed mandarin fruit and identified based on internal transcribed spacer sequence and morphological characteristics. Of the 197 isolates, 182 (92.4%) were identified as Mucor piriformis, 7 (3.6%) were M. circinelloides (6 M. circinelloides f. lusitanicus and 1 M. circinelloides f. circinelloides), 4 (2%) were M. racemosus f. racemosus, 3 (1.5%) were M hiemalis, and 1 (0.5%) was M. mucedo. All species grew at 0 and 5 degrees C, except M. circinelloides, which did not grow at 0 degrees C. Mycelia' growth was arrested at 27 degrees C for M. piriformis; 35 degrees C for M. racemosus f. racemosus, M. circinelloides f lusitanicus, M. hiemalis and M. mucedo; and 37 degrees C for M. circinelloides f. circinelloides. Optimal mycelial growth occurred at 20 degrees C for M. pinfornzis and M. mucedo, 25 degrees C for M. racemosus f. racemosus and M. hiemalis, 27 C for M. circinelloides f. lusitanicus, and 30 degrees C for M. circinelloides f. circinelloides. M. piriformis grew significantly faster than the other four species at 5 and 20 degrees C, and M. nzucedo was the slowest in growth among the five species. Sporangiospores of M. piriformis, M. racemosus f. racemosus, and M. hiemalis germinated at both 5 and 20 degrees C. M. circinelloides germinated at 20 degrees C but did not germinate at 5 degrees C after incubation for 48 h. All five Mucor spp. caused decay on mandarin fruit inoculated with the fungi, and the lesion size caused by M. piriformis was significantly larger than that caused by other species at both 5 and 20 degrees C. Our results indicated that Mucor rot in mandarin fruit in California is caused by Mucor spp. consisting of M. piriformis, M. circinelloides, M. racemosus f. racemosus, M. hiemalis, and M. mucedo, with M. piriformis being the dominant and most virulent species. Previously, M. racemosus was reported on citrus. This is the first report of Mucor rot in citrus caused by M. piriformis, M. circinelloides, M. hiemalis, and M. mucedo. C1 [Saito, S.; Xiao, C. L.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Michailides, T. J.] Univ Calif, Kearney Agr Res & Extens Ctr, Parlier, CA 93648 USA. RP Xiao, CL (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. EM Chang-Lin.Xiao@ars.usda.gov FU Citrus Research Board FX This research was supported, in part, by the Citrus Research Board. We thank T. Ruiz, who first recognized the importance of this pathogen to the citrus industry, for his valuable contributions and continued cooperation; J. L. Smilanick, D. A. Margosan, and K. Fjeld for technical assistance; and the personnel of the three citrus packinghouses for assistance in sample collection. NR 38 TC 0 Z9 0 U1 4 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2016 VL 100 IS 6 BP 1054 EP 1063 DI 10.1094/PDIS-10-15-1173-RE PG 10 WC Plant Sciences SC Plant Sciences GA DL4UV UT WOS:000375634200003 ER PT J AU McCollum, G Hilf, M Irey, M Luo, WQ Gottwald, T AF McCollum, Greg Hilf, Mark Irey, Mike Luo, Weiqi Gottwald, Tim TI Susceptibility of Sixteen Citrus Genotypes to 'Candidatus Liberibacter asiaticus' SO PLANT DISEASE LA English DT Article ID SWEET ORANGE CULTIVARS; SAO-PAULO STATE; HUANGLONGBING DISEASE; 1ST REPORT; ROOTSTOCK; TOLERANCE; FLORIDA; BRAZIL; MANAGEMENT; RESISTANCE AB Huanglongbing (HLB) disease is the most serious threat to citrus production worldwide and, in the last decade, has devastated the Florida citrus industry. In the United States, HLB is associated with the phloem-limited alpha-proteobacterium 'Candidatus Liberibacter asiaticus' and its insect vector, the Asian citrus psyllid (ACP; Diaphorina cirri). Significant effort is being put forth to develop novel citrus germplasm that has a lower propensity to succumb to HLB than do currently available varieties. Effective methods of screening citrus germplasm for susceptibility to HLB are essential. In this study, we exposed small, grafted trees of 16 citrus types to free-ranging ACP vectors and 'Ca. L. asiaticus' inoculum in the greenhouse. During 45 weeks of exposure to ACP, the cumulative incidence of 'Ca.. L. asiaticus' infection was 70%. Trees of Citrus macrophylla and C. medica were most susceptible to 'Ca. L. asiaticus', with 100% infection by the end of the test period in three trials, while the complex genetic hybrids 'US 1-4-59' and 'Fallglo' consistently were least susceptible, with approximately 30% infection. Results obtained in this greenhouse experiment showed good agreement with trends observed in the orchard, supporting the validity of our approach for screening citrus germplasm for susceptibility to HLB. C1 [McCollum, Greg; Hilf, Mark; Gottwald, Tim] USDA ARS, Ft Pierce, FL 34945 USA. [Irey, Mike] Southern Gardens Citrus, Clewiston, FL 33440 USA. [Luo, Weiqi] N Carolina State Univ, Ctr Integrated Pest Management, Raleigh, NC 27606 USA. RP McCollum, G (reprint author), USDA ARS, Ft Pierce, FL 34945 USA. EM greg.mccollum@ars.usda.gov NR 45 TC 1 Z9 2 U1 4 U2 13 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2016 VL 100 IS 6 BP 1080 EP 1086 DI 10.1094/PDIS-08-15-0940-RE PG 7 WC Plant Sciences SC Plant Sciences GA DL4UV UT WOS:000375634200006 ER PT J AU Malapi-Wight, M Salgado-Salazar, C Demers, JE Clement, DL Rane, KK Crouch, JA AF Malapi-Wight, Martha Salgado-Salazar, Catalina Demers, Jill E. Clement, David L. Rane, Karen K. Crouch, Jo Anne TI Sarcococca Blight: Use of Whole-Genome Sequencing for Fungal Plant Disease Diagnosis SO PLANT DISEASE LA English DT Article ID SPORED GRAMINICOLOUS COLLETOTRICHUM; MEDIATED ISOTHERMAL AMPLIFICATION; RICE BLAST FUNGUS; CALONECTRIA-PSEUDONAVICULATA; CYLINDROCLADIUM-BUXICOLA; VIRUS DIAGNOSTICS; 1ST REPORT; REVEALS; GENES; PHYLOGENY AB Early and accurate diagnosis of new plant pathogens is vital for the rapid implementation of effective mitigation strategies and appropriate regulatory responses. Most commonly, pathogen identification relies on morphology and DNA marker analysis. However, for new diseases, these approaches may not be sufficient for precise diagnosis. In this study, we used whole-genome sequencing (WGS) to identify the causal agent of a new disease affecting Sarcococca hookeriana (sarcococca). Blight symptoms were observed on sarcococca and adjacent Buxus sempervirens (boxwood) plants in Maryland during 2014. Symptoms on sarcococca were novel, and included twig dieback and dark lesions on leaves and stems. A Calonectria sp. was isolated from both hosts and used to fulfill Koch's postulates but morphology and marker sequence data precluded species-level identification. A 51.4-Mb WGS was generated for the two isolates and identified both as Calonectria pseudonaviculata. A single-nucleotide polymorphism at a noncoding site differentiated between the two host isolates. These results indicate that the same C. pseudonaviculata genotype has the ability to induce disease on both plant species. This study marks the first application of WGS for fungal plant pathogen diagnosis and demonstrates the power of this approach to rapidly identify causal agents of new diseases. C1 [Malapi-Wight, Martha] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Salgado-Salazar, Catalina] USDA ARS, Systemat Mycol & Microbiol Lab, New Brunswick, NJ 08906 USA. [Salgado-Salazar, Catalina] Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08906 USA. [Demers, Jill E.] USDA ARS, Systemat Mycol & Microbiol Lab, Ellicott City, MD 21042 USA. [Clement, David L.] Univ Maryland, Home & Garden Informat Ctr, Ellicott City, MD 21042 USA. [Rane, Karen K.] Univ Maryland, Plant Diagnost Lab, College Pk, MD 20742 USA. [Crouch, Jo Anne] USDA ARS, Systemat Mycol & Microbiol Lab, Washington, DC 20250 USA. [Malapi-Wight, Martha] USDA, Anim & Plant Hlth Inspect Serv, Plant Germplasm Quarantine Program, Beltsville, MD 20705 USA. RP Crouch, JA (reprint author), USDA ARS, Systemat Mycol & Microbiol Lab, Washington, DC 20250 USA. EM joanne.crouch@ars.usda.gov OI Crouch, Jo Anne/0000-0001-6886-8090; Salgado Salazar, Catalina/0000-0002-4156-692X FU USDA Animal and Plant Health Inspection Service Plant Protection and Quarantine (APHIS-PPQ) Farm Bill grants; USDA-ARS Floriculture and Nursery Initiative; USDA-ARS project [8042-22000-279]; USDA-ARS Headquarters Research Associate fellowship; USDA-APHIS-PPQ Farm Bill grants FX This research was funded by 2013-2015 USDA Animal and Plant Health Inspection Service Plant Protection and Quarantine (APHIS-PPQ) Farm Bill grants and USDA-ARS Floriculture and Nursery Initiative funding to J. A. Crouch, and USDA-ARS project 8042-22000-279; M. Malapi-Wight was supported by a class of 2013 USDA-ARS Headquarters Research Associate fellowship to J. A. Crouch; C. Salgado-Salazar was supported by 2014-2015 USDA-APHIS-PPQ Farm Bill grants to J. A. Crouch; and J. E. Demers was supported by a class of 2013 USDA-ARS Headquarters Research Associate fellowship to L. Castlebury. We thank A. Gould for her continued partnership and support and for her ongoing mentorship of C. Salgado-Salazar. NR 55 TC 1 Z9 1 U1 2 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2016 VL 100 IS 6 BP 1093 EP 1100 DI 10.1094/PDIS-10-15-1159-RE PG 8 WC Plant Sciences SC Plant Sciences GA DL4UV UT WOS:000375634200008 ER PT J AU Rahmatov, M Rouse, MN Steffenson, BJ Andersson, SC Wanyera, R Pretorius, ZA Houben, A Kumarse, N Bhavani, S Johansson, E AF Rahmatov, Mahbubjon Rouse, Matthew N. Steffenson, Brian J. Andersson, Staffan C. Wanyera, Ruth Pretorius, Zacharias A. Houben, Andreas Kumarse, Nazari Bhavani, Sridhar Johansson, Eva TI Sources of Stem Rust Resistance in Wheat-Alien Introgression Lines SO PLANT DISEASE LA English DT Article ID F-SP TRITICI; RYE TRANSLOCATION LINES; ADULT-PLANT RESISTANCE; SECALE-CEREALE L.; SPRING WHEAT; RACE UG99; AESTIVUM L.; CONFERRING RESISTANCE; SEEDLING RESISTANCES; FOOD SECURITY AB Stem rust is one of the most devastating diseases of wheat. Widely virulent races of the pathogen in the Ug99 lineage (e.g., TTKSK) are threatening wheat production worldwide; therefore, there is an urgent need to enhance the diversity of resistance genes in the crop. The objectives of this study were to identify new sources of resistance in wheat-alien introgression derivatives from Secale cereale, Leymus mollis, L. racemosus, and Thinopyrum junceiforme, postulate genes conferring the resistance, and verify the postulated genes by use of molecular markers. From seedling tests conducted in the greenhouse, the presence of seven known stem rust resistance genes (Sr7b, Sr8a, Sr9d, Sr10, Sr31, Sr36, and SrSatu) was postulated in the wheat-alien introgression lines. More lines possessed a high level of resistance in the field compared with the number of lines that were resistant at the seedling stage. Three 2R (2D) wheat-rye substitution lines (SLU210, SLU238, and SLU239) seemed likely to possess new genes for resistance to stem rust based on their resistance pattern to 13 different stem rust races but the genes responsible could not be identified. Wheat-rye, wheat-L. racemosus, and wheat-L. mollis substitutions or translocations with single and multiple interchanges of chromosomes, in particular of the B and D chromosomes of wheat, were verified by a combination of genomic in situ hybridization and molecular markers. Thus, the present study identified novel resistance genes originating from different alien introgressions into the wheat genome of the evaluated lines. Such genes may prove useful in enhancing the diversity of stem rust resistance in wheat against widely virulent pathogen races such as those in the Ug99 lineage. C1 [Rahmatov, Mahbubjon] Swedish Univ Agr Sci, Dept Plant Breeding, SE-23053 Alnarp, Sweden. [Rahmatov, Mahbubjon] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Rahmatov, Mahbubjon] Tajik Agr Univ, Dushanbe 734017, Tajikistan. [Rouse, Matthew N.] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Rouse, Matthew N.; Steffenson, Brian J.] Univ Minnesota, Dept Plant Pathol, Njoro, Kenya. [Andersson, Staffan C.] Swedish Univ Agr Sci, Dept Plant Breeding, Njoro, Kenya. [Wanyera, Ruth] Kenyan Agr & Livestock Res Org, Food Crops Res Ctr, Njoro, Kenya. [Pretorius, Zacharias A.] Univ Orange Free State, Dept Plant Sci, ZA-9300 Bloemfontein, South Africa. [Houben, Andreas] Leibniz Inst Plant Genet & Crop Plant Res, D-06466 Gatersleben, Stadt Seeland, Germany. [Kumarse, Nazari] Aegean Agr Res Inst, Reg Cereal Rust Res Ctr, Izmir, Turkey. [Bhavani, Sridhar] Int Maize & Wheat Improvement Ctr, ICRAF House, Nairobi, Kenya. [Johansson, Eva] Swedish Univ Agr Sci, Dept Plant Breeding, S-90183 Umea, Sweden. RP Rahmatov, M (reprint author), Swedish Univ Agr Sci, Dept Plant Breeding, SE-23053 Alnarp, Sweden.; Rahmatov, M (reprint author), Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA.; Rahmatov, M (reprint author), Tajik Agr Univ, Dushanbe 734017, Tajikistan. EM mahbubjon@gmail.com RI Rouse, Matthew/G-5474-2011; OI Steffenson, Brian/0000-0001-7961-5363 FU Monsanto's Beachell-Borlaug International Scholars Program; Swedish University of Agricultural Sciences and K. Nazari at ICARDA, Izmir, Turkey; USDA-ARS Appropriated Project [5062-21220-021-00]; USDA-ARS National Plant Disease Recovery System; Durable Rust Resistance in Wheat project; Lieberman-Okinow Endowment at the University of Minnesota; Bill & Melinda Gates Foundation; U.K. Department for International Development FX We thank Monsanto's Beachell-Borlaug International Scholars Program for financial support of M. Rahmatov; Swedish University of Agricultural Sciences and K. Nazari at ICARDA, Izmir, Turkey; USDA-ARS Appropriated Project 5062-21220-021-00, USDA-ARS National Plant Disease Recovery System, and the Durable Rust Resistance in Wheat project for support of M. Rouse; the Lieberman-Okinow Endowment at the University of Minnesota, the Bill & Melinda Gates Foundation, and the U.K. Department for International Development to Cornell University for the Borlaug Global Rust Initiative Durable Rust Resistance in Wheat Project for support of B. Steffenson; A. Case for conducting the APR assessments in St. Paul; and K. Kumke for technical assistance during GISH analysis. NR 78 TC 1 Z9 1 U1 4 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2016 VL 100 IS 6 BP 1101 EP 1109 DI 10.1094/PDIS-12-15-1448-RE PG 9 WC Plant Sciences SC Plant Sciences GA DL4UV UT WOS:000375634200009 ER PT J AU Dung, JKS Alderman, SC Walenta, DL Hamm, PB AF Dung, Jeremiah K. S. Alderman, Stephen C. Walenta, Darrin L. Hamm, Philip B. TI Spatial Patterns of Ergot and Quantification of Sclerotia in Perennial Ryegrass Seed Fields in Eastern Oregon SO PLANT DISEASE LA English DT Article ID CLAVICEPS-PURPUREA; KENTUCKY BLUEGRASS; COUNT DATA; BINOMIAL-DISTRIBUTION; DISEASE INCIDENCE; GERMINATION; SURVIVAL; FUNGUS; WHEAT; ASSOCIATION AB Ergot, caused by Claviceps purpurea, is a major disease of perennial ryegrass grown for seed in eastern Oregon. The objective of this research was to quantify and describe the spatial patterns of ergot severity in each of three 50-ha commercial fields of perennial ryegrass grown for seed in 2012 and 2013. In total, 1,433 and 1,405 quadrats were sampled among the three fields in 2012 and 2013, respectively, and the percentage of quadrats with ergot ranged from 59 to 90%. The mean incidence of infected seed heads in each quadrat ranged between 13 and 29%, while mean severity in each quadrat ranged from 0.2 to 0.5 sclerotia per seed head. Significant autocorrelation and clustering were observed in all three fields in both years, as indicated by Moran's I and spatial analysis by distance indices of aggregation. The mean number of ergot sclerotia collected from each field after harvest ranged between 4 and 15 sclerotia m(-2) in 2012 and 18 and 119 sclerotia m(-2) in 2013. Sclerotia left in perennial fields after harvest are a significant source of inoculum that should be targeted for control. This is the first study to quantify spatial patterns of ergot in perennial ryegrass and provides insights into possible mechanisms that contribute to ergot etiology and epidemiology. C1 [Dung, Jeremiah K. S.] Oregon State Univ, Cent Oregon Agr Res Ctr, Dept Bot & Plant Pathol, Madras, Tamil Nadu, India. [Alderman, Stephen C.] USDA ARS, Natl Forage Seed Prod Res Ctr, Corvallis, OR USA. [Walenta, Darrin L.] Union Cty Extens Ctr, Dept Crop & Soil Sci, La Grande, OR USA. [Hamm, Philip B.] Oregon State Univ, Hermiston Agr Res & Extens Ctr, Dept Bot & Plant Pathol, Hermiston, OR USA. RP Dung, JKS (reprint author), Oregon State Univ, Cent Oregon Agr Res Ctr, Dept Bot & Plant Pathol, Madras, Tamil Nadu, India. EM jeremiah.dung@oregonstate.edu FU Columbia Basin Grass Seed Growers Association; Columbia River Seeds LLC; DLF International Seeds; Oregon Seed Council; Simplot Soil Builders; United States Department of Agriculture; Union County Seed Growers Association; Washington State Commission on Pesticide Registration; Washington Turf Grass Seed Commission FX We thank J. Almaguer, J. Holcomb, and P. Rogers for providing technical assistance; D. Johnson and T. Peever for helpful comments on the manuscript; Hawman Farms in Hermiston, OR for providing study sites and equipment; and Riverview Seed for graciously providing in-kind support. Funding for this research was provided by the Columbia Basin Grass Seed Growers Association, Columbia River Seeds LLC, DLF International Seeds, the Oregon Seed Council, Simplot Soil Builders, the United States Department of Agriculture, the Union County Seed Growers Association, the Washington State Commission on Pesticide Registration, and the Washington Turf Grass Seed Commission. NR 51 TC 0 Z9 0 U1 4 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2016 VL 100 IS 6 BP 1110 EP 1117 DI 10.1094/PDIS-08-14-0787-RE PG 8 WC Plant Sciences SC Plant Sciences GA DL4UV UT WOS:000375634200010 ER PT J AU Gent, DH Probst, C Nelson, ME Grove, GG Massie, ST Twomey, MC AF Gent, David H. Probst, Claudia Nelson, Mark E. Grove, Gary G. Massie, Stephen T. Twomey, Megan C. TI Interaction of Basal Foliage Removal and Late-Season Fungicide Applications in Management of Hop Powdery Mildew SO PLANT DISEASE LA English DT Article ID LEAF REMOVAL; DOWNY MILDEW; SEVERITY; GRAPE AB Canopy management is an important aspect of control of powdery mildew diseases and may influence the intensity of fungicide applications required to suppress disease. In hop, powdery mildew (caused by Podosphaera macularis) is most damaging to cones when infection occurs during bloom and the juvenile stages of cone development. Experiments were conducted over 3 years to evaluate whether fungicide applications could be ceased after the most susceptible stages of cone development (late July) without unduly affecting crop yield and quality when disease pressure was moderated with varying levels of basal foliage removal. In experimental plots of 'Galena' hop, the incidence of leaves with powdery mildew was similar whether fungicides were ceased in late July or made in late August. Disease levels on leaves were unaffected by the intensity of basal foliage removal, whereas the intensity of basal foliage removal interacted with the duration of fungicide applications to affect disease levels on cones. Similar experiments conducted in large plots of 'Tomahawk' hop in a commercial hop yard similarly found no significant impact on disease levels on leaves from either the duration of fungicide applications or intensity of basal foliage removal. In contrast, on cones, application of fungicides into August had a modest, suppressive effect on powdery mildew. There was also some evidence that the level of powdery mildew on cones associated with fungicide treatment was influenced by the intensity of basal foliage removal. When fungicide applications ceased in late July, there was a progressive decrease in the incidence of cones with powdery mildew with increasing intensity of basal foliage removal. Removing basal foliage two to three times allowed fungicide applications to be terminated in late July rather than late August without diminishing disease control on cones, yield, or cone quality factors. Thus, this study further establishes that fungicide applications made during the early stages of hop cone development have the strongest effect on suppression of powdery mildew on cones. The additive effect of fungicide applications targeted to the periods of greatest cone susceptibility and canopy management to reduce disease favorability may obviate the need for fungicide applications later in the season. This appears to be a viable strategy in mature hop yards of certain cultivars when disease pressure is not excessively high. C1 [Gent, David H.] USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. [Gent, David H.; Twomey, Megan C.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Probst, Claudia; Nelson, Mark E.; Grove, Gary G.] Washington State Univ, Dept Plant Pathol, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. [Massie, Stephen T.] Pharsalia Hop Co, Zionville, NC 28698 USA. RP Gent, DH (reprint author), USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA.; Gent, DH (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM Dave.Gent@ars.usda.gov FU Washington State University Agriculture Research Center; Hop Research Council; United States Department of Agriculture Agricultural Research Service [CRIS 5358-21000-040-00]; Washington State Department of Agriculture Specialty Crops Research Block Grant Program FX Financial support for this research was provided by Washington State University Agriculture Research Center, Hop Research Council, United States Department of Agriculture Agricultural Research Service CRIS 5358-21000-040-00, and Washington State Department of Agriculture Specialty Crops Research Block Grant Program. We thank E. Lizotte for providing helpful suggestions that improved this article; and John I. Haas, Inc. for providing resources and facilities that made this work possible. NR 36 TC 0 Z9 0 U1 1 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2016 VL 100 IS 6 BP 1153 EP 1160 DI 10.1094/PDIS-10-15-1232-RE PG 8 WC Plant Sciences SC Plant Sciences GA DL4UV UT WOS:000375634200016 ER PT J AU Rosskopf, E Hong, J AF Rosskopf, E. Hong, J. TI First Report of Bacterial Stem Rot of "Heirloom" Tomatoes Caused by Pectobacterium carotovorum subsp brasiliensis in Florida SO PLANT DISEASE LA English DT News Item C1 [Rosskopf, E.; Hong, J.] ARS, USDA, Ft Pierce, FL 34982 USA. RP Rosskopf, E (reprint author), ARS, USDA, Ft Pierce, FL 34982 USA. NR 3 TC 1 Z9 1 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2016 VL 100 IS 6 BP 1233 EP 1233 DI 10.1094/PDIS-09-15-0961-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DL4UV UT WOS:000375634200028 ER PT J AU Juzwik, OJ McDermott-Kubeczko, M Stewart, TJ Ginzel, MD AF Juzwik, Ohio. J. McDermott-Kubeczko, M. Stewart, T. J. Ginzel, M. D. TI First Report of Geosmithia morbida on Ambrosia Beetles Emerged From Thousand Cankers-diseased Juglans nigra in Ohio SO PLANT DISEASE LA English DT News Item C1 [Juzwik, Ohio. J.] US Forest Serv, USDA, No Res Stn, St Paul, MN USA. [McDermott-Kubeczko, M.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Stewart, T. J.; Ginzel, M. D.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. RP Juzwik, OJ (reprint author), US Forest Serv, USDA, No Res Stn, St Paul, MN USA. NR 2 TC 1 Z9 1 U1 3 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2016 VL 100 IS 6 BP 1238 EP 1238 DI 10.1094/PDIS-10-15-1155-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DL4UV UT WOS:000375634200041 ER PT J AU Jia, Y McClung, A AF Jia, Y. McClung, A. TI First Report of Multiple Races of Rice Blast Fungus Magnaporthe oryzae in Puerto Rico SO PLANT DISEASE LA English DT News Item ID RESISTANCE C1 [Jia, Y.; McClung, A.] ARS, USDA, DB NRRC, Stuttgart, AK USA. RP Jia, Y (reprint author), ARS, USDA, DB NRRC, Stuttgart, AK USA. NR 5 TC 0 Z9 0 U1 4 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2016 VL 100 IS 6 BP 1242 EP 1242 DI 10.1094/PDIS-12-15-1391-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DL4UV UT WOS:000375634200053 ER PT J AU Yan, GP Plaisance, A Huang, D Upadhaya, A Gudmestad, NC Handoo, ZA AF Yan, G. P. Plaisance, A. Huang, D. Upadhaya, A. Gudmestad, N. C. Handoo, Z. A. TI First Report of the Stubby Root Nematode Paratrichodorus allius on Potato in North Dakota SO PLANT DISEASE LA English DT News Item ID CORKY RINGSPOT C1 [Yan, G. P.; Plaisance, A.; Huang, D.; Upadhaya, A.; Gudmestad, N. C.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Handoo, Z. A.] USDA ARS, Nematol Lab, Beltsville, MD 20705 USA. RP Yan, GP (reprint author), N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. NR 5 TC 1 Z9 1 U1 4 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2016 VL 100 IS 6 BP 1247 EP 1247 DI 10.1094/PDIS-11-15-1350-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DL4UV UT WOS:000375634200065 ER PT J AU Rivera, Y Salgado-Salazar, C Creswell, TC Ruhl, G Crouch, JA AF Rivera, Y. Salgado-Salazar, C. Creswell, T. C. Ruhl, G. Crouch, J. A. TI First Report of Downy Mildew Caused by Peronospora sp on Agastache sp in the United States SO PLANT DISEASE LA English DT News Item C1 [Rivera, Y.; Salgado-Salazar, C.; Crouch, J. A.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Rivera, Y.; Salgado-Salazar, C.] Rutgers State Univ, Dept Plant Biol & Pathol, Brunswick, NJ 08901 USA. [Creswell, T. C.; Ruhl, G.] Purdue Univ, Plant & Pest Diagnost Lab, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA. RP Rivera, Y (reprint author), USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA.; Rivera, Y (reprint author), Rutgers State Univ, Dept Plant Biol & Pathol, Brunswick, NJ 08901 USA. OI Salgado Salazar, Catalina/0000-0002-4156-692X NR 4 TC 1 Z9 1 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2016 VL 100 IS 6 BP 1249 EP 1250 DI 10.1094/PDIS-10-15-1119-PDN PG 2 WC Plant Sciences SC Plant Sciences GA DL4UV UT WOS:000375634200071 ER PT J AU Xiao, L Li, YY Tan, GL Lan, PX Zhong, L Liu, Y Li, R Li, F AF Xiao, L. Li, Y. Y. Tan, G. L. Lan, P. X. Zhong, L. Liu, Y. Li, R. Li, F. TI First Report of Zucchini tigre mosaic virus Infecting Several Cucurbit Plants in China SO PLANT DISEASE LA English DT News Item C1 [Xiao, L.; Li, Y. Y.; Lan, P. X.; Li, F.] Yunnan Agr Univ, Educ Minist China, Key Lab Agrobiodivers & Pest Management, Kunming 650201, Peoples R China. [Tan, G. L.] Yunnan Agr Univ, Modern Educ Technol Ctr, Kunming 650201, Peoples R China. [Zhong, L.] Yunnan Acad Agr Sci, Hort Crops Res Inst, Kunming 650205, Peoples R China. [Liu, Y.] Hunan Acad Agr Sci, Inst Plant Protect, Changsha 410125, Hunan, Peoples R China. [Li, R.] USDA ARS, Natl Germplasm Resources Lab, Beltsville, MD 20705 USA. RP Xiao, L (reprint author), Yunnan Agr Univ, Educ Minist China, Key Lab Agrobiodivers & Pest Management, Kunming 650201, Peoples R China. NR 3 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2016 VL 100 IS 6 BP 1253 EP 1254 DI 10.1094/PDIS-11-15-1318-PDN PG 2 WC Plant Sciences SC Plant Sciences GA DL4UV UT WOS:000375634200082 ER PT J AU Anderson, RL AF Anderson, Randy L. TI Suppressing weed growth after wheat harvest with underseeded red clover in organic farming SO RENEWABLE AGRICULTURE AND FOOD SYSTEMS LA English DT Article DE cover crops; cultural tactics; downy brome; no-till; weed biomass ID WINTER ANNUAL WEEDS; RADISH COVER CROP; NO-TILL; GREAT-PLAINS; SYSTEMS; MANAGEMENT; CORN; AGRICULTURE; EMERGENCE AB Organic producers are seeking alternative tactics for weed control so that they can reduce their need for tillage. In this study, we examined cover crop strategies for suppressing weed growth after harvest of wheat. Three cover crop treatments, red clover (mammoth type), a mixture of oat and dry pea, and a control were compared. Treatments were established in both winter and spring wheat, resulting in six treatments arranged in a randomized complete block design. Red clover was underseeded in wheat by drilling in the spring, and the oat/pea mixture was planted in August. Oat was planted uniformly across all treatments in the following growing season. The red clover treatment effectively suppressed weeds, reducing post-harvest weed biomass, density of volunteer winter wheat, and seed production of downy brome by more than 99% compared with the control. Oat/pea was not effective for weed management, likely because of less fall growth and competition compared with red clover. Underseeding red clover did not affect winter wheat yield, but reduced spring wheat yield by 17%. Oat yield, however, was reduced by volunteer crop plants and downy brome infestations in all treatments. Underseeding clovers in winter wheat may effectively manage weeds and, if they winterkill, can replace the need for tillage to control weeds after wheat harvest. C1 [Anderson, Randy L.] USDA ARS, Brookings, SD 57006 USA. RP Anderson, RL (reprint author), USDA ARS, Brookings, SD 57006 USA. EM randy.anderson@ars.usda.gov NR 29 TC 0 Z9 0 U1 12 U2 27 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1742-1705 EI 1742-1713 J9 RENEW AGR FOOD SYST JI Renew. Agr. Food Syst. PD JUN PY 2016 VL 31 IS 3 BP 185 EP 190 DI 10.1017/S1742170515000022 PG 6 WC Agriculture, Multidisciplinary SC Agriculture GA DL8CJ UT WOS:000375866700001 ER PT J AU Eggleston, G Heckemeyer, M St Cyr, E Wartelle, L AF Eggleston, Gillian Heckemeyer, Matthew St Cyr, Eldwin Wartelle, Lynda TI Case Study: Commercialization of Sweet Sorghum Juice Clarification for Large-Scale Syrup Manufacture SO SUGAR TECH LA English DT Article DE Sweet sorghum; Starch; Juice clarification; Large-scale processing; Commercial syrup manufacture ID RAW SUGAR; FERMENTATION; CANE AB The precipitation and burning of insoluble starch granules from sweet sorghum juice on heating coils prevented the large-scale manufacture of syrup at a new industrial plant in Missouri, USA. To remove insoluble starch granules, a series of small and large-scale experiments were conducted at the plant on raw juice extracted by roller milling from two commercial sweet sorghum cultivars Honey Drip and M81E. Both starch concentration and granule size contributed to processing problems. Leaving de-aerated raw juice in settling tanks before clarification settled out a considerable amount of small to medium sized starch granules, but did not remove enough starch. Hot clarification of juice performed better than cold clarification in removing starch and turbid particles. During the USDA hot clarification process (heat to 80 degrees C; lime to pH 6.5 with milk of lime [360 g/L]; 5 ppm polyanionic flocculant) in the plant, some starch was solubilized. Small to large size starch granules were removed in the clarification mud which improved as settling time progressed. At least 1 h of settling is recommended in the plant. Late in the processing season, scum formed on the surface of the juice that did not contain any starch granules, but did contain microscopic bubbles making the scum less dense than juice and unprecipitable. This highlights sweet sorghum quality differences across the processing season that still need to be addressed along with cultivar differences. Overall, the introduction of juice de-aeration, settling, and clarification steps enabled the large-scale manufacture of syrup and helped to mitigate quality and processing differences due to cultivar and environmental effects and thus assured continual supply to customers. C1 [Eggleston, Gillian; St Cyr, Eldwin; Wartelle, Lynda] USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [Heckemeyer, Matthew] Heckemeyer Mills, Sikeston, MO USA. RP Eggleston, G (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM gillian.eggleston@ars.usda.gov FU United Sorghum Checkoff Program FX The authors thank Mr. John Zatlokovicz for helping set-up and calibrate the portable laboratory instruments, and the United Sorghum Checkoff Program for partial funding of this research. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 19 TC 2 Z9 2 U1 4 U2 7 PU SPRINGER INDIA PI NEW DELHI PA 7TH FLOOR, VIJAYA BUILDING, 17, BARAKHAMBA ROAD, NEW DELHI, 110 001, INDIA SN 0972-1525 J9 SUGAR TECH JI Sugar Tech. PD JUN PY 2016 VL 18 IS 3 BP 249 EP 257 DI 10.1007/s12355-015-0387-9 PG 9 WC Agronomy SC Agriculture GA DL7AG UT WOS:000375790600004 ER PT J AU Zhang, XH Zhong, YJ Zhou, ZH Liu, Y Zhang, HM Chen, F Chen, WG Xie, QM AF Zhang, Xinheng Zhong, Yangjin Zhou, Zhenhai Liu, Yang Zhang, Huanmin Chen, Feng Chen, Weiguo Xie, Qingmei TI Molecular characterization, phylogeny analysis and pathogenicity of a Muscovy duck adenovirus strain isolated in China in 2014 SO VIROLOGY LA English DT Article DE Duck adenovirus 3; Virus particles; Physicochemical properties; Sequence analysis; Pathogenicity ID COMPLETE DNA-SEQUENCE; DROP SYNDROME VIRUS; FOWL ADENOVIRUSES; AVIAN ADENOVIRUS; GENOME SEQUENCE; AVIADENOVIRUS; GENUS; ORGANIZATION; PROPOSAL; POULTRY AB This study aimed to characterize a novel adenovirus (AdV) isolated from diseased Muscovy ducks in China. After the AdV was successfully propagated in duck embryo fibroblasts, the morphological and physicochemical properties of the virions were studied by electron microscopy and different tests. The results of the analyses were in conformity with AdV properties. The full genome sequence was determined and analyzed. The new isolate (named CH-GD-12-2014) shared over 91% sequence identity with duck AdV-2 representing the species Duck aviadenovirus B. The most important distinguishing feature between the two DAdV strains was the presence of a second fiber gene in the Chinese isolate. Phylogeny reconstruction confirmed the affiliation of the virus with goose and duck AdVs in the genus Aviadenovirus. Experimental infection resulted in embryo death, and intramuscular inoculation provoked morbidity and mortality among ducks and chickens. (C) 2016 Published by Elsevier Inc. C1 [Zhang, Xinheng; Zhong, Yangjin; Zhou, Zhenhai; Liu, Yang; Chen, Feng; Chen, Weiguo; Xie, Qingmei] South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China. [Zhang, Xinheng; Zhong, Yangjin; Zhou, Zhenhai; Liu, Yang; Chen, Feng; Chen, Weiguo; Xie, Qingmei] Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China. [Zhang, Huanmin] ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. [Zhang, Xinheng; Zhong, Yangjin; Zhou, Zhenhai; Liu, Yang; Chen, Feng; Chen, Weiguo; Xie, Qingmei] Key Lab Anim Hlth Aquaculture & Environm Control, Guangzhou 510642, Guangdong, Peoples R China. [Zhang, Xinheng; Chen, Weiguo; Xie, Qingmei] South China Collaborat Innovat Ctr Poultry Dis Co, Guangzhou 510642, Guangdong, Peoples R China. RP Xie, QM (reprint author), South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China.; Xie, QM (reprint author), Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China. EM qmx@scau.edu.cn FU National Modern Agricultural Industry Technology System Project of China [CARS-41]; Guangdong Province Science and Technology Plan Project [2012B020306002, 2012B091100078] FX This study was supported by the National Modern Agricultural Industry Technology System Project of China (CARS-41) and Guangdong Province Science and Technology Plan Project (Grant nos. 2012B020306002 and 2012B091100078). NR 29 TC 0 Z9 1 U1 0 U2 1 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD JUN PY 2016 VL 493 BP 12 EP 21 DI 10.1016/j.virol.2016.03.004 PG 10 WC Virology SC Virology GA DL7MH UT WOS:000375824300002 PM 26989945 ER PT J AU Montalva, C Collier, K Luz, C Humber, RA AF Montalva, Cristian Collier, Karin Luz, Christian Humber, Richard A. TI Pandora bullata (Entomophthoromycota: Entomophthorales) affecting calliphorid flies in central Brazil SO ACTA TROPICA LA English DT Article DE Diptera; Natural enemy; Entomopathogenic fungi; Entomophthoromycota; Chrysomya ID RIO-DE-JANEIRO; CHRYSOMYA-MEGACEPHALA; HUMAN MYIASIS; BIOLOGICAL RESERVE; MUSCA-DOMESTICA; 1ST REPORT; DIPTERA; FUNGI; BACTERIA; THAILAND AB Fungi are where one finds them, and if one seeks fungal pathogens affecting flies, then a garbage dump may be an ideal place to find both persistent, abundant fly populations and their fungal pathogens. An obvious fungal epizootic affecting the oriental latrine fly, Chrysomya megacephala (Diptera: Calliphoridae), was observed over several days in mid-February 2015 at the local garbage dump adjacent to the city of Cavalcante, northern Goias. This site harbored large populations of both C. megacephala and a Musca sp. (Diptera: Muscidae) but only the population of oriental latrine fly was affected by any fungal pathogen and presented unusually dense populations of fresh cadavers. The fungus was identifiable as Pandora bullata (Entomophthorales: Entomophthoraceae) only after a very small number of characteristically decorated resting spores were found in these flies two months later; this represents the first Brazilian (and South American) record of this species. P. bullata is known previously from a small number of North American, European and Australian collections, all of which have included relatively abundant production of resting spores. We cannot dismiss the possibility that the extremely sparse formation of resting spores at this Brazilian site may be due to abiotic factors such as latitude (13 degrees 46'40.53"S), day length, ambient temperatures, or even the precipitation patterns in this mid-tropical montaine site. Epizootic events affecting calliphorids in Brazil strengthen the interest in entomophthoran pathogens for biological control of flies. (C) 2016 Elsevier B.V. All rights reserved. C1 [Montalva, Cristian; Luz, Christian] Univ Fed Goias, IPTSP, LPI, BR-74690900 Goiania, Go, Brazil. [Collier, Karin] Ctr Univ Gurupi UnirG, Gurupi, Tocantins, Brazil. [Humber, Richard A.] USDA ARS, Emerging Pests & Pathogens Res Unit, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Luz, C (reprint author), Univ Fed Goias, Inst Patol Trop & Saude Publ, Ave Esperanca S-N,Campus Samambaia, BR-74690900 Goiania, Go, Brazil. EM wchrisluz@hotmail.com FU CAPES (Coordination of the Improvement of Higher Education) Science Without Borders project [PVE 71/2013, 149988] FX This study was supported by CAPES (Coordination of the Improvement of Higher Education) Science Without Borders project PVE 71/2013, 149988. NR 33 TC 0 Z9 1 U1 3 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0001-706X EI 1873-6254 J9 ACTA TROP JI Acta Trop. PD JUN PY 2016 VL 158 BP 177 EP 180 DI 10.1016/j.actatropica.2016.03.007 PG 4 WC Parasitology; Tropical Medicine SC Parasitology; Tropical Medicine GA DK8GU UT WOS:000375166000024 PM 26968351 ER PT J AU Ortega, MS Denicol, AC Cole, JB Null, DJ Hansen, PJ AF Ortega, M. S. Denicol, A. C. Cole, J. B. Null, D. J. Hansen, P. J. TI Use of single nucleotide polymorphisms in candidate genes associated with daughter pregnancy rate for prediction of genetic merit for reproduction in Holstein cows SO ANIMAL GENETICS LA English DT Article DE dairy cow; fertility; genetic evaluation and reproduction; single nucleotide polymorphisms ID EMBRYONIC SURVIVAL RATES; DAIRY-COWS; ARTIFICIAL-INSEMINATION; FERTILITY; CATTLE; EXPRESSION; ENDOMETRIAL; ESTRADIOL; FERTILIZATION; PROGESTERONE AB We evaluated 69 SNPs in genes previously related to fertility and production traits for their relationship to daughter pregnancy rate (DPR), cow conception rate (CCR) and heifer conception rate (HCR) in a separate population of Holstein cows grouped according to their predicted transmitting ability (PTA) [-1 (n=1287) and 1.5 (n=1036)] for DPR. Genotyping was performed using Sequenom MassARRAY((R)). There were a total of 39 SNPs associated with the three fertility traits. The SNPs that explained the greater proportion of the genetic variation for DPR were COQ9 (3.2%), EPAS1 (1.0%), CAST (1.0%), C7H19orf60 (1.0%) and MRPL48 (1.0%); for CCR were GOLGA4 (2.4%), COQ9 (1.8%), EPAS1 (1.1%) and MRPL48 (0.8%); and for HCR were HSD17B7 (1.0%), AP3B1 (0.8%), HSD17B12 (0.7%) and CACNA1D (0.6%). Inclusion of 39 SNPs previously associated with DPR in the genetic evaluation system increased the reliability of PTA for DPR by 0.20%. Many of the genes represented by SNPs associated with fertility are involved in steroidogenesis or are regulated by steroids. A large proportion of SNPs previously associated with genetic merit for fertility in Holstein bulls maintained their association in a separate population of cows. The inclusion of these genes in genetic evaluation can improve reliabilities of genomic estimates for fertility. C1 [Ortega, M. S.; Denicol, A. C.; Hansen, P. J.] Univ Florida, Dept Anim Sci, DH Barron Reprod & Perinatal Biol Res Program, POB 110910, Gainesville, FL 32611 USA. [Ortega, M. S.; Denicol, A. C.; Hansen, P. J.] Univ Florida, Genet Inst, POB 110910, Gainesville, FL 32611 USA. [Cole, J. B.; Null, D. J.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD USA. RP Hansen, PJ (reprint author), Univ Florida, Dept Anim Sci, DH Barron Reprod & Perinatal Biol Res Program, POB 110910, Gainesville, FL 32611 USA.; Hansen, PJ (reprint author), Univ Florida, Genet Inst, POB 110910, Gainesville, FL 32611 USA. EM pjhansen@ufl.edu RI Cole, John/J-8571-2014; Zhygunov, Dmytro/D-1372-2015 OI Cole, John/0000-0003-1242-4401; Zhygunov, Dmytro/0000-0001-9435-2266 FU Agriculture and Food Research Initiative Competitive from USDA National Institute of Food and Agriculture [2013-68004-20365]; Southeast Milk Inc. Milk Check-off Program; 'Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information' of the Agricultural Research Service of the United States Department of Agriculture [1265-31000-096-00] FX Research was supported by Agriculture and Food Research Initiative Competitive Grant no. 2013-68004-20365 from the USDA National Institute of Food and Agriculture and a grant from the Southeast Milk Inc. Milk Check-off Program. Cole and Null were supported by appropriated project 1265-31000-096-00, 'Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information', of the Agricultural Research Service of the United States Department of Agriculture. NR 46 TC 2 Z9 2 U1 3 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0268-9146 EI 1365-2052 J9 ANIM GENET JI Anim. Genet. PD JUN PY 2016 VL 47 IS 3 BP 288 EP 297 DI 10.1111/age.12420 PG 10 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA DK5WJ UT WOS:000374991500002 PM 26923315 ER PT J AU Pittol, M Durso, L Valiati, VH Fiuza, LM AF Pittol, Michele Durso, Lisa Valiati, Victor Hugo Fiuza, Lidia Mariana TI Agronomic and environmental aspects of diazotrophic bacteria in rice fields SO ANNALS OF MICROBIOLOGY LA English DT Review DE Microbial community; Biofertilizer; Paddies; Nitrogen fixation ID GROWTH-PROMOTING RHIZOBACTERIA; NITROGEN-FIXING BACTERIA; ORYZA-SATIVA L.; LEGUMINOSARUM BV TRIFOLII; SOIL MICROBIAL COMMUNITIES; PLANT-GROWTH; ENDOPHYTIC BACTERIA; N-2 FIXATION; PADDY FIELDS; PHOSPHATE SOLUBILIZATION AB This article provides an overview of the free-living and plant-associated nitrogen (N)-fixing bacterial communities in wet rice fields, with a focus on describing the elements affecting community assemblages in this waterlogged soil-plant system. Nitrogen is a crucial nutrient for rice yield and growth. Characteristics of the rice paddy ecosystem promote N-fertilizer losses, resulting in negative impacts on the environment. Public concerns on sustainable rice crop production and food security have accentuated interest in exploring biological supplementary nitrogen sources. Biological N-fixation is a significant source of the nitrogen in agroecosystems. The nitrogen requirement of rice crops can be partly remedied by managing and promoting the activities of N-fixing microorganisms. These changes are leading towards a cleaner approach that maintains sustainability while simultaneously improving crop production targets. The use of N-fixing microorganisms as biofertilizers and the factors driving the success of this technology in wet rice paddies are also discussed. C1 [Pittol, Michele; Valiati, Victor Hugo; Fiuza, Lidia Mariana] Univ Vale Rio Sinos UNISINOS, Programa Posgrad Biol, 950 Unisinos Ave, Sao Leopoldo, RS, Brazil. [Durso, Lisa] ARS, USDA, Agroecosyst Management Res Unit, 251 Filley Hall,UNL East Campus, Lincoln, NE USA. RP Pittol, M (reprint author), Univ Vale Rio Sinos UNISINOS, Programa Posgrad Biol, 950 Unisinos Ave, Sao Leopoldo, RS, Brazil. EM mipittoll@gmail.com OI Valiati, Victor Hugo/0000-0002-4467-4547 NR 165 TC 0 Z9 0 U1 10 U2 34 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1590-4261 EI 1869-2044 J9 ANN MICROBIOL JI Ann. Microbiol. PD JUN PY 2016 VL 66 IS 2 BP 511 EP 527 DI 10.1007/s13213-015-1154-6 PG 17 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DK6QX UT WOS:000375051100001 ER PT J AU Chatvijitkul, S Davis, DA Lim, CE AF Chatvijitkul, Sirirat Davis, D. Allen Lim, Chhorn E. TI Lipid extracted distillers dried grains with solubles (LE-DDGS) as a partial replacement for soybean meal in hybrid tilapia (Oreochromis niloticus x O. aureus) diets SO AQUACULTURE LA English DT Article DE Distillers dried grains; Alternative protein sources; Nutrient retention; Hybrid tilapia; Soybean meal ID ETHANOL COPRODUCTS; FEED-UTILIZATION; CHANNEL CATFISH; GROWTH-RESPONSE; NILE TILAPIA; PROTEIN; CORN; FRY; COMBINATION; RESISTANCE AB Feed costs are primarily driven by the cost of protein sources in the feed. Substitution of expensive protein sources with lower cost ingredients would potentially reduce feed cost. Lipid-extracted distillers dried grains with solubles (LE-DDGS) is a relatively new product of distillers dried grains with solubles (DDGS) which could be used as an alternative protein source in tilapia feed formulations. Two growth trials were conducted to evaluate the substitution of soybean meal with LE-DDGS in practical diets for hybrid tilapia (Oreochromis niloticus x Oreochromis aureus). The first trial evaluated five levels of LE-DDGS (0, 200, 300, 400 and 500 g kg(-1)) in a practical diet containing 360 g kg(-1) protein and 60 g kg(-1) lipid. The result indicated that increasing levels of LE-DDGS generally resulted in a reduced growth of hybrid tilapia with a significant (P < 0.05) depression in performance when incorporated in the diet at 400 and 500 g kg(-1). The second study evaluated four levels of inclusion (0, 200, 400 and 500 g kg(-1)) with lysine supplements and a fifth diet with an additional 20 g kg(-1) lipid (27 g kg(-1) lysine and 80 g kg(-1) lipid). The result demonstrated that all diets promoted good performance of hybrid tilapia. In conclusion, LE-DDGS could be a promising protein source as a substitute for soybean meal at levels up to 300 g kg(-1) of the diet. With lysine and possible lipid supplement, LE-DDGS as much as 500 g kg(-1) produced good performance in hybrid tilapia diet. As the ethanol industry continues to refine their process and shift the composition of co-products that are sold on the market, it is recommended that the new product should re-evaluated for their efficiency. Statement of relevance: The present study can show that DDGS can be used as a substitution of soybean meal in tilapia fish. Given the rise in soybean meal prices, DDGS can be used in aquacultural feeds along with supplementation of lysine as a replacement of the more expensive plant derived protein source. (C) 2016 Elsevier B.V. All rights reserved. C1 [Chatvijitkul, Sirirat; Davis, D. Allen] Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, 203 Swingle Hall, Auburn, AL 36849 USA. [Lim, Chhorn E.] USDA ARS, Aquat Anim Hlth Res Unit, Auburn, AL 36831 USA. RP Davis, DA (reprint author), Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, 203 Swingle Hall, Auburn, AL 36849 USA. EM davisda@auburn.edu FU Dakota Gold Poet FX The authors would like to extend their thanks to those who have taken time to critically review this manuscript as well as those who helped in supporting this research at E. W. Shell Fisheries Research Station in Auburn University, AL. This research was conducted with cooperation and support from Dakota Gold Poet. NR 36 TC 0 Z9 0 U1 9 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD JUN 1 PY 2016 VL 459 BP 131 EP 136 DI 10.1016/j.aquaculture.2016.03.023 PG 6 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA DK2TK UT WOS:000374767100017 ER PT J AU Craft, CD Ross, C Sealey, WM Gaylord, TG Barrows, FT Fornshell, G Myrick, CA AF Craft, Christopher D. Ross, Carolyn Sealey, Wendy M. Gaylord, T. Gibson Barrows, Frederic T. Fornshell, Gary Myrick, Christopher A. TI Growth, proximate composition, and sensory characteristics of Rainbow Trout Oncorhynchus mykiss consuming alternative proteins SO AQUACULTURE LA English DT Article DE Rainbow Trout; Nutrition; Growth; Alternative proteins; Sensory analysis ID FISH-MEAL REPLACEMENT; PLANT-PROTEINS; SOYBEAN-MEAL; APPARENT DIGESTIBILITY; ATLANTIC SALMON; DIETS; PERFORMANCE; INGREDIENTS; FEEDSTUFFS; EFFICIENCY AB Increases in the price of fishmeal have spurred increases in the cost of aquafeeds to fish production facilities. This intensified a search for alternatives to fish meal. In this study, eight experimental aquafeeds were formulated from 4 ingredient groups that excluded fishmeal. These ingredient groups were designed as an animal product diet (APD), a novel plant protein diet (NPD), a plant products with future potential diet (PIP), and a plant product diet (PPD). These diets were compared to two standard fishmeal based diets (FMD). Each ingredient group was formulated into high protein (45%) and low protein (40%) diets and used in a 5 x 2 factorial feeding trial. Diets were randomly assigned to three replicate tanks containing 15 juvenile Rainbow Trout Oncorhynchus mykiss (mean initial weight = 91.3 g). After the growth trial fish from each treatment were used in a sensory analysis, examining the aroma, flavor, and texture of the fillets being harvested. The 40% protein FMD produced both the highest final wet weight: 441 +/- 5.7 g (mean +/- SEM) and lowest feed conversion ratio 0.93 +/- 0.02 g feed consumedlg weight gain (mean +/- SEM). However, the best performing experimental diets had comparable values. More differences in growth metrics were noted among different feed ingredient groups than between protein levels. Proximate analyses were also used to determine proportions of moisture, protein, lipid, and energy within a subsample of fish from each treatment. These analyses indicated protein sparing and lipid sparing by fish consuming the 40% protein diets and 45% protein diets, respectively. Sensory analyses identified few significant differences in fillet characteristics among the different treatments. The results indicate that diets composed solely of plant-based ingredients can promote similar rates of Rainbow Trout growth, produce high quality fillets, and be less costly to fish producers. Statement of relevance We show that alternative feeds efficiently grow trout. (C) 2016 Elsevier B.V. All rights reserved. C1 [Craft, Christopher D.; Myrick, Christopher A.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Campus Delivery 1474, Ft Collins, CO 80523 USA. [Ross, Carolyn] Washington State Univ, Dept Food Sci & Human Nutr, FSHN 1222,POB 646376, Pullman, WA 99164 USA. [Sealey, Wendy M.; Gaylord, T. Gibson] USFWS, Bozeman Fish Technol Ctr, 4050 Bridger Canyon Rd, Bozeman, MT 59715 USA. [Barrows, Frederic T.] USDA ARS, Trout Grains Project, 3509F Natl Fish Hatchery Rd, Hagerman, ID 83332 USA. [Fornshell, Gary] Univ Idaho Extens, 630 Addison Ave West,Suite 1600, Twin Falls, ID 83301 USA. RP Craft, CD (reprint author), Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Campus Delivery 1474, Ft Collins, CO 80523 USA. EM ccraft@geiconsultants.com; cfross@wsu.edu; wendy_sealey@fws.gov; gibson_gaylord@fws.gov; rick.barrows@ars.usda.gov; gafornsh@uidaho.edu; chris.myrick@colostate.edu FU Western Regional Aquaculture Center from the USDA National Institute of Food and Agriculture [2006-38500-17048, 2010-38500-21758] FX This project was supported by Western Regional Aquaculture Center Grant nos. 2006-38500-17048 and 2010-38500-21758 from the USDA National Institute of Food and Agriculture. Personnel from the Western Regional Aquaculture Center assisted with all phases of the study and report. The authors thank J. Callison, K. Oles, B. Robey, T. Swarr, B. Welk, N. Wilson, I. Wilson., and R. Winkelman for their considerable assistance with tank system construction, fish care, data collection, and troubleshooting of any laboratory problems that arose, even at inconvenient times. Additional thanks to O. Aon, J. Anderson, R. Dana, S. Dugan, A. Ficke, E. Gardunio, S. Royer, L. Hargis, A. Schwindt, A. Herdrich, A. McMahon, A. Milano, D. Pruitt, A. Riverman, S. Sedillo, N. Vieira, J. Wardell, and Z. Underwood for their assistance. We also wish to thank A. Hess and J. zumBrunnen of the Franklin A. Graybill Statistical Laboratory at Colorado State University for help with statistical consulting and data analysis. All animal care and handling protocols were approved by the Colorado State University Institutional Animal Care and Use Committee protocol 10-2318 A, and personnel were trained as required by the committee. The authors have no conflicts of interest to disclose. NR 47 TC 0 Z9 0 U1 17 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD JUN 1 PY 2016 VL 459 BP 223 EP 231 DI 10.1016/j.aquaculture.2016.03.039 PG 9 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA DK2TK UT WOS:000374767100031 ER PT J AU Silva, JAA Hall, DG Gottwald, TR Andrade, MS Maldonado, W Alessandro, RT Lapointe, SL Andrade, EC Machado, MA AF Silva, Jose A. A. Hall, David G. Gottwald, Timothy R. Andrade, Moacir S. Maldonado, Walter, Jr. Alessandro, Rocco T. Lapointe, Stephen L. Andrade, Eduardo C. Machado, Marcos A. TI Repellency of selected Psidium guajava cultivars to the Asian citrus psyllid, Diaphorina citri SO CROP PROTECTION LA English DT Article DE Huanglongbing; Citrus greening; Candidatus Liberibacter; Guava ID ESSENTIAL OIL COMPOSITION; GUAVA LEAF VOLATILES; HEMIPTERA PSYLLIDAE; DISEASE; LEAVES; MANAGEMENT; KUWAYAMA; EXOTICA; SHOOTS; VECTOR AB Huanglongbing (HLB) is the most devastating disease of citrus worldwide. It is caused by bacteria of the genus 'Candidatus Liberibacter' and transmitted by two psyllid species, the Asian citrus psyllid (ACP) Diaphorina citri, and the African citrus psyllid Trioza erytreae. Considerable research has been conducted toward developing and implementing HLB and ACP management strategies. With respect to ACP control, of interest is that reports indicate guava, Psidium guajava, can be repellent to ACP. We conducted research to further evaluate repellency of guava to ACP. In one set of experiments, guava oil from five Brazilian guava cultivars ('J3', 'Pedro Sato', 'Seculo XXI', 'Thailand' and 'Paluma') was extracted from leaves (immature and mature) by hydro-distillation in a Clevenger-type apparatus and evaluated for psyllid repellency. In a second set of experiments, repellency of guava leaves to ACP was investigated using leaves (immature and mature) from two guava cultivars in Florida, 'Pink' and 'Thai White'. In each set of experiments, repellency was evaluated by releasing ACP adults into a cage with two large vials, one containing a young flush shoot (= immature leaves) of Murraya exotica (a favored host plant of the psyllid, the flush of which is highly attractive to ACP) and one with M. exotica flush and the test material of interest (guava oil, immature guava leaf or mature guava leaf). The adults were free to move throughout the cage and into the vials, and the number of psyllids in each vial was counted after 24 h. The results showed that all guava materials tested had at least some repellency to ACP. Mature leaves tended to have a greater repellent effect than immature leaves. Each of the five oils exhibited repellency. A report in the literature suggested that sulfur compounds associated with guava may be responsible for ACP repellency. Interestingly, the five guava oil extracts we studied were repellent to ACP but none contained any sulfur compounds. Identification of the constituents responsible for repellency could lead to new ACP management tactics. (C) 2016 Published by Elsevier Ltd. C1 [Silva, Jose A. A.; Machado, Marcos A.] APTA IAC, Agr Secretary Sao Paulo State, Caixa Postal 35, BR-14770000 Colina, SP, Brazil. [Hall, David G.; Gottwald, Timothy R.; Alessandro, Rocco T.; Lapointe, Stephen L.] ARS, USDA, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. [Andrade, Moacir S.] UFSCAR Fed Univ Sao Carlos, Dept Chem, Sao Carlos, SP, Brazil. [Maldonado, Walter, Jr.] UNESP Univ Sao Paulo State, Dept Stat, Sao Paulo, Brazil. [Andrade, Eduardo C.] EMBRAPA Cassava & Fruits, Sao Paulo, Brazil. RP Hall, DG (reprint author), ARS, USDA, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM jaalbertosilva@gmail.com; David.Hall@ars.usda.gov; Tim.Gottwald@ars.usda.gov; msandrade2003@gmail.com; walter@agroestat.com.br; Rocco.Alessandro@ars.usda.gov; Stephen.Lapointe@ars.usda.gov; eandrade@cnpmf.embrapa.br; Marcos@centrodecitricultura.br RI Maldonado Jr, Walter/J-6894-2016; OI Maldonado Jr, Walter/0000-0003-2837-3012; Alessandro, Rocco/0000-0001-7983-0557 NR 26 TC 1 Z9 1 U1 7 U2 23 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD JUN PY 2016 VL 84 BP 14 EP 20 DI 10.1016/j.cropro.2016.02.006 PG 7 WC Agronomy SC Agriculture GA DK3FA UT WOS:000374801200003 ER PT J AU Yang, CY Powell, CA Duan, YP Shatters, RG Lin, YJ Zhang, MQ AF Yang, Chuanyu Powell, Charles A. Duan, Yongping Shatters, Robert G. Lin, Youjian Zhang, Muqing TI Mitigating citrus huanglongbing via effective application of antimicrobial compounds and thermotherapy SO CROP PROTECTION LA English DT Article DE Huanglongbing; Chemo-thermotherapy; Delivery method ID CANDIDATUS LIBERIBACTER ASIATICUS; GREENING DISEASE; VALIDAMYCIN-A; PLANTS; AMERICANUS; TOLERANCE; MOLECULES; BACTERIUM; TREES; ROT AB Huanglongbing (HLB) is a serious citrus disease that threatens the citrus industry worldwide. HLB is a systemic, infectious disease and the putative causal bacterium Candidatus liberibacter asiaticus (Las) resides in citrus phloem. In this study, the effects of heat treatment, chemical formulations, and application methods on bacterial titer in the citrus host, measured by quantitative PCR, were evaluated. Thermotherapy at 45 degrees C (Ct = 30.79 +/- 6.26) was more effective against Las than thermotherapy at 40 degrees C (Ct = 28.72 +/- 6.00) or 42 degrees C (Ct = 28.02 +/- 6.25), and enhanced the delivery efficiency of effective chemical compounds into the citrus phloem. Ampicillin sodium (Amp) and a combination of actidione and validoxylamine A (Act + VA) were the most effective chemical formulations against Las. When Amp or Act + VA treatment was combined with thermotherapy, the area under disease progress curve standardized (AUDPCs) was significantly lower than the thermotherapy treatments alone. The Las titer of Amp and Act + VA treatment applied by bark paint were significantly reduced, compared with those of Amp or Act + VA applied by root drench. Therefore, we propose a chemo thermotherapy strategy coupled with a bark paint application method for disease control in HLB-infected citrus plants. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Yang, Chuanyu; Zhang, Muqing] Guangxi Univ, State Key Lab Conversat & Utilizat Subtrop Arobio, Nanning 530005, Guangxi, Peoples R China. [Yang, Chuanyu; Powell, Charles A.; Lin, Youjian; Zhang, Muqing] Univ Florida, IRREC IFAS, Ft Pierce, FL 34945 USA. [Duan, Yongping; Shatters, Robert G.] USDA ARS, USHRL, Ft Pierce, FL 34945 USA. [Yang, Chuanyu; Zhang, Muqing] Fujian Agr & Forestry Univ, Coll Crop Sci, Fuzhou 350002, Peoples R China. RP Zhang, MQ (reprint author), Guangxi Univ, State Key Lab Conversat & Utilizat Subtrop Arobio, Nanning 530005, Guangxi, Peoples R China. EM mqzhang@ufl.edu FU US Citrus Research and Development Foundation (CRDF) [584]; Guangxi Natural Science Foundation [2013GXNSFCB019002]; Guangxi Programs for science and technology development [347004-7] FX We greatly appreciate Bioscience Editing Solutions for critically reading this paper and providing helpful suggestions. Financial support was provided by US Citrus Research and Development Foundation (project CRDF#584), Guangxi Natural Science Foundation (project 2013GXNSFCB019002) and Guangxi Programs for science and technology development (project 347004-7). NR 44 TC 1 Z9 1 U1 12 U2 22 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD JUN PY 2016 VL 84 BP 150 EP 158 DI 10.1016/j.cropro.2016.03.013 PG 9 WC Agronomy SC Agriculture GA DK3FA UT WOS:000374801200020 ER PT J AU Baker, BH Kroger, R Prevost, JD Pierce, T Ramirez-Avila, JJ Czarnecki, JMP Faust, D Flora, C AF Baker, B. H. Kroger, R. Prevost, J. D. Pierce, T. Ramirez-Avila, J. J. Czarnecki, J. M. Prince Faust, D. Flora, C. TI A field-scale investigation of nutrient and sediment reduction efficiencies of a low-technology best management practice: Low-grade weirs SO ECOLOGICAL ENGINEERING LA English DT Article DE Agriculture; Best management practice; Nutrients; Sediment; Conservation ID AGRICULTURAL DRAINAGE DITCHES; MISSISSIPPI RIVER-BASIN; GULF-OF-MEXICO; WATER-QUALITY; HYDROLOGICAL VARIABILITY; CONSTRUCTED WETLANDS; NITROGEN; LOSSES; LANDSCAPES; DYNAMICS AB Increasing awareness of hypoxia in ocean regions across the globe has led to creation of nutrient reduction strategies targeting this coastal problem. In the Mississippi River Basin, the Governor's Action Plan has called for a 45% load reduction of both nitrogen (N) and phosphorous (P) to reduce the Gulf of Mexico hypoxic zone. One documented best management practice (BMP) for nutrient reduction is low-grade weirs (hereafter "weirs"). Recent studies have highlighted advantages of using low-grade weirs in agricultural ditches for controlled drainage by increasing hydraulic residence time (HRT) and mitigating nutrient loading from storm water and sedimentation. This study aimed to assess the ability of weirs to reduce nutrients in agriculture runoff at field-scale in the Mississippi Delta. Nutrient load reductions were observed in drainage ditches with and without weirs. However, nutrient and sediment loads varied widely, with observed load reductions ranging from -885 to 96% and -1 to 65% for total inorganic N and total inorganic P, respectively, in ditches with weirs. Maximum nutrient load reductions highlight that systems, with and without weirs, have the capability to reduce nutrients under certain conditions, while minimum nutrient reductions highlight when drainage ditch capacity limitations were exceeded. Differences in nutrient and sediment concentrations between storm- and low-flow samples ranged from 28 to 97%, indicating water velocity as the driving force behind observed differences. Seasonal analysis of nutrient runoff revealed significantly higher concentrations of total inorganic N, total inorganic P, and total suspended solids in the spring (p = 0.003, p < 0.0001, and p < 0.0001, respectively), while an N also increased in the fall (p = 0.007). Differences in annual sediment and P trends showed lower concentrations in systems with weirs. While this investigation highlighted both the successes and limitations of utilizing low-grade weirs as a BMP, results suggest that capture capacity of BMPs should be tailored to drainage acreage and site variability. (C) 2016 Elsevier B.V. All rights reserved. C1 [Baker, B. H.; Czarnecki, J. M. Prince; Faust, D.] Mississippi State Univ, Dept Wildlife Fisheries & Aquaculture, Mississippi State, MS 39762 USA. [Kroger, R.] Covington Civil & Environm LLC, 2510 14th St,Suite 1010, Gulfport, MS 39501 USA. [Prevost, J. D.] Delta FARM, POB 276, Stoneville, MS 38776 USA. [Pierce, T.] Environm Protect Agcy Gulf Mexico Program Off, 2510 14th St,Suite 1212, Gulfport, MS 39501 USA. [Ramirez-Avila, J. J.] Mississippi State Univ, Dept Civil & Environm Engn, Mississippi State, MS 39762 USA. [Flora, C.] USDA ARS, Stoneville, MS 38776 USA. [Baker, B. H.] Mississippi State Univ, Water Qual Lab, Thompson Hall 253, Starkville, MS 39762 USA. RP Baker, BH (reprint author), Mississippi State Univ, Dept Wildlife Fisheries & Aquaculture, Mississippi State, MS 39762 USA.; Baker, BH (reprint author), Mississippi State Univ, Water Qual Lab, Thompson Hall 253, Starkville, MS 39762 USA. EM beth.baker@msstate.edu RI Falkievicz, Bruno/M-4255-2016 FU Environmental Protection Agency Gulf of Mexico Program Office [MX-95460110]; Mississippi Agricultural Forestry and Experiment Station; Forest and Wildlife Research Center FX The authors would like to gratefully acknowledge funding from the Environmental Protection Agency Gulf of Mexico Program Office (Grant No. MX-95460110), Delta F.A.R.M., and study location land owners. Authors would also like to thank the Mississippi Agricultural Forestry and Experiment Station, and Forest and Wildlife Research Center for support. NR 39 TC 2 Z9 2 U1 13 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-8574 EI 1872-6992 J9 ECOL ENG JI Ecol. Eng. PD JUN PY 2016 VL 91 BP 240 EP 248 DI 10.1016/j.ecoleng.2016.02.038 PG 9 WC Ecology; Engineering, Environmental; Environmental Sciences SC Environmental Sciences & Ecology; Engineering GA DK2TE UT WOS:000374766500031 ER PT J AU Niu, SL Classen, AT Dukes, JS Kardol, P Liu, LL Luo, YQ Rustad, L Sun, J Tang, JW Templer, PH Thomas, RQ Tian, DS Vicca, S Wang, YP Xia, JY Zaehle, S AF Niu, Shuli Classen, Aimee T. Dukes, Jeffrey S. Kardol, Paul Liu, Lingli Luo, Yiqi Rustad, Lindsey Sun, Jian Tang, Jianwu Templer, Pamela H. Thomas, R. Quinn Tian, Dashuan Vicca, Sara Wang, Ying-Ping Xia, Jianyang Zaehle, Soenke TI Global patterns and substrate-based mechanisms of the terrestrial nitrogen cycle SO ECOLOGY LETTERS LA English DT Review DE Leaching; mineralisation; nitrification; nitrogen deposition; nitrogen loss; plant N uptake; saturation; soil N retention; threshold ID FOREST ECOSYSTEMS; NITRATE UPTAKE; ATMOSPHERIC DEPOSITION; PHOSPHORUS LIMITATION; TEMPERATE FORESTS; UPTAKE KINETICS; CARBON-DIOXIDE; NEW-ENGLAND; OAK FOREST; SATURATION AB Nitrogen (N) deposition is impacting the services that ecosystems provide to humanity. However, the mechanisms determining impacts on the N cycle are not fully understood. To explore the mechanistic underpinnings of N impacts on N cycle processes, we reviewed and synthesised recent progress in ecosystem N research through empirical studies, conceptual analysis and model simulations. Experimental and observational studies have revealed that the stimulation of plant N uptake and soil retention generally diminishes as N loading increases, while dissolved and gaseous losses of N occur at low N availability but increase exponentially and become the dominant fate of N at high loading rates. The original N saturation hypothesis emphasises sequential N saturation from plant uptake to soil retention before N losses occur. However, biogeochemical models that simulate simultaneous competition for soil N substrates by multiple processes match the observed patterns of N losses better than models based on sequential competition. To enable better prediction of terrestrial N cycle responses to N loading, we recommend that future research identifies the response functions of different N processes to substrate availability using manipulative experiments, and incorporates the measured N saturation response functions into conceptual, theoretical and quantitative analyses. C1 [Niu, Shuli; Sun, Jian; Tian, Dashuan] Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Ecosyst Network Observat & Modeling, Beijing, Peoples R China. [Classen, Aimee T.] Univ Copenhagen, Nat Hist Museum Denmark, Univ Pk 15, DK-2100 Copenhagen O, Denmark. [Dukes, Jeffrey S.] Purdue Univ, Dept Forestry & Nat Resources, Dept Biol Sci, Purdue Climate Change Res Ctr, W Lafayette, IN 47907 USA. [Kardol, Paul] Swedish Univ Agr Sci, Dept Forest Ecol & Management, S-90183 Umea, Sweden. [Liu, Lingli] Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing, Peoples R China. [Luo, Yiqi] Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK 73019 USA. [Rustad, Lindsey] US Forest Serv, No Res Stn, Durham, NH USA. [Tang, Jianwu] Marine Biol Lab, Ctr Ecosyst, Woods Hole, MA 02543 USA. [Templer, Pamela H.] Boston Univ, Dept Biol, 5 Cummington St, Boston, MA 02215 USA. [Thomas, R. Quinn] Virginia Tech, Dept Forest Resources & Environm Conservat, Blacksburg, VA USA. [Vicca, Sara] Univ Antwerp, Dept Biol, Res Grp Plant & Vegetat Ecol, B-2610 Antwerp, Belgium. [Wang, Ying-Ping] CSIRO, Ocean & Atmosphere, PMB 1, Aspendale, Vic 3195, Australia. [Xia, Jianyang] E China Normal Univ, Tiantong Natl Stn Forest Ecosyst, Shanghai 200062, Peoples R China. [Xia, Jianyang] E China Normal Univ, Sch Ecol & Enivronm Sci, Shanghai 200062, Peoples R China. [Xia, Jianyang] E China Normal Univ, Res Ctr Global Change & Ecol Forecasting, Shanghai 200062, Peoples R China. [Zaehle, Soenke] Max Planck Inst Biogeochem, Biogeochem Integrat Dept, Hans Knoll Str 10, D-07745 Jena, Germany. RP Niu, SL (reprint author), Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Ecosyst Network Observat & Modeling, Beijing, Peoples R China. EM sniu@igsnrr.ac.cn RI Liu, Lingli/A-7771-2008; Dukes, Jeffrey/C-9765-2009; Tang, Jianwu/K-6798-2014; Vicca, Sara/I-3637-2012; Classen, Aimee/C-4035-2008; wang, yp/A-9765-2011; Zaehle, Sonke/C-9528-2017; publicationpage, cmec/B-4405-2017 OI Liu, Lingli/0000-0002-5696-3151; Dukes, Jeffrey/0000-0001-9482-7743; Tang, Jianwu/0000-0003-2498-9012; Vicca, Sara/0000-0001-9812-5837; Classen, Aimee/0000-0002-6741-3470; Zaehle, Sonke/0000-0001-5602-7956; FU National Natural Science Foundation of China [31470528, 31420103917, 31290220]; Ministry of Science and Technology of China [2013CB956300]; Thousand Youth Talents Plan Project; INTERFACE Research Coordination Network [US NSF DEB-0955771]; Research Group of Global Change Ecology at Henan University [NSFC41030104/D0308]; Swedish Research Council Formas FX Preparation of the manuscript was financially supported by the National Natural Science Foundation of China (31470528, 31420103917, 31290220), the Ministry of Science and Technology of China (2013CB956300) and the Thousand Youth Talents Plan Project. This paper is a product of the workshop 'Using results from global change experiments to inform land model development and calibration', co-sponsored by the INTERFACE Research Coordination Network (US NSF DEB-0955771) and the Research Group of Global Change Ecology at Henan University (NSFC41030104/D0308). SV is a postdoctoral research associate of the Fund for Scientific Research - Flanders (FWO). PK acknowledges support from the Swedish Research Council Formas. NR 89 TC 3 Z9 3 U1 71 U2 139 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-023X EI 1461-0248 J9 ECOL LETT JI Ecol. Lett. PD JUN PY 2016 VL 19 IS 6 BP 697 EP 709 DI 10.1111/ele.12591 PG 13 WC Ecology SC Environmental Sciences & Ecology GA DL6MD UT WOS:000375752400011 PM 26932540 ER PT J AU Washburn, BE Swearingin, RM Pullins, CK Rice, ME AF Washburn, Brian E. Swearingin, Ryan M. Pullins, Craig K. Rice, Matthew E. TI Composition and Diversity of Avian Communities Using a New Urban Habitat: Green Roofs SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Airport; Bird strike; Green roof; Habitat; Urban ecology; Wildlife ID MANAGEMENT; SAFETY; AREAS; BIRDS; SYSTEMS; GULLS AB Green roofs on buildings are becoming popular and represent a new component of the urban landscape. Public benefits of green roof projects include reduced stormwater runoff, improved air quality, reduced urban heat island effects, and aesthetic values. As part of a city-wide plan, several green roofs have been constructed at Chicago's O'Hare International Airport (ORD). Like some other landscaping features, green roofs on or near an airport might attract wildlife and thus increase the risk of bird-aircraft collisions. During 2007-2011, we conducted a series of studies to evaluate wildlife use of newly constructed green roofs and traditional (gravel) roofs on buildings at ORD. These green roofs were 0.04-1.62 ha in area and consisted of primarily stonecrop species for vegetation. A total of 188 birds were observed using roofs during this research. Of the birds using green roofs, 66, 23, and 4 % were Killdeer, European Starlings, and Mourning Doves, respectively. Killdeer nested on green roofs, whereas the other species perched, foraged, or loafed. Birds used green roofs almost exclusively between May and October. Overall, avian use of the green roofs was minimal and similar to that of buildings with traditional roofs. Although green roofs with other vegetation types might offer forage or cover to birds and thus attract potentially hazardous wildlife, the stonecrop-vegetated green roofs in this study did not increase the risk of bird-aircraft collisions. C1 [Washburn, Brian E.] Anim Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, 6100 Columbus Ave, Sandusky, OH 44870 USA. [Swearingin, Ryan M.; Pullins, Craig K.; Rice, Matthew E.] Anim Plant Hlth Inspect Serv, USDA, Wildlife Serv, Chicago, IL USA. RP Washburn, BE (reprint author), Anim Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, 6100 Columbus Ave, Sandusky, OH 44870 USA. EM brian.e.washburn@aphis.usda.gov FU Chicago's O'Hare International Airport; O'Hare Modernization Program FX Access to facilities was graciously provided by Chicago's O'Hare International Airport and the FedEx Corporation. The U.S. Department of Agriculture, Animal Plant Health Inspection Service, Wildlife Services National Wildlife Research Center Institutional Animal Care and Use Committee approved procedures involving wildlife (QA-1928). Chicago's O'Hare International Airport and the O'Hare Modernization Program provided funding and logistical support. We thank S. Beckerman, T. DeVault, and two anonymous reviewers for helpful comments on this manuscript. NR 55 TC 0 Z9 0 U1 19 U2 43 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X EI 1432-1009 J9 ENVIRON MANAGE JI Environ. Manage. PD JUN PY 2016 VL 57 IS 6 BP 1230 EP 1239 DI 10.1007/s00267-016-0687-1 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK3QG UT WOS:000374831700007 PM 26956765 ER PT J AU Ding, C Khir, R Pan, ZL Wood, DF Tu, K El-Mashad, H Berrios, J AF Ding, Chao Khir, Ragab Pan, Zhongli Wood, Delilah F. Tu, Kang El-Mashad, Hamed Berrios, Jose TI Improvement in Storage Stability of Infrared-Dried Rough Rice SO FOOD AND BIOPROCESS TECHNOLOGY LA English DT Article DE Rice; Infrared drying; Storage stability; Physicochemical properties; Thermal properties; Cooking quality ID DIFFERENT TEMPERATURES; WAXY RICE; PHYSICOCHEMICAL PROPERTIES; PASTING PROPERTIES; MILLING QUALITY; STORED RICE; COOKING; GELATINIZATION; ATTRIBUTES; FLOUR AB Infrared radiation heating has a promising potential in improving drying rate and food safety, but its effect on the storage stability of rough rice is not known. The objective of this study was to develop an infrared drying (IRD) method to improve the storage stability of rough rice during storage. The effects of IRD on the physicochemical properties of stored rough rice were compared with those of hot air drying (HAD) and ambient air drying (AAD). Freshly harvested M206 rice was dried to a targeted moisture content of 16 % (d.b.) by using IRD, HAD, and AAD. The dried rice samples were then stored at 35.0 +/- 1.0 A degrees C and a relative humidity of 65.0 A +/- 3.0 % for up to 10 months. The physicochemical and cooking properties of rice samples were periodically determined over the storage duration. Compared with AAD, the yellowness index, water uptake, and volume expansion ratio of the rough rice dried with IRD and stored for 4 months were reduced by 26.3, 76.3, and 14.5 %, respectively. After 10 months of storage, the change in hardness of cooked IR-dried rice was significantly reduced by 22 % compared to that of samples dried with AAD. IRD likely caused a slight denaturation of protein and annealing of starch that was located on the surface layer of rice kernels, resulting in decreased gelatinization temperature, enthalpy, and viscosity, and reduced the changes in microstructure, but retained cooking characteristics after storage. Therefore, IRD is recommended as a promising technique that achieves high rice drying efficiency and improved storage stability. C1 [Ding, Chao] Nanjing Univ Finance & Econ, Coll Food Sci & Engn, 3 Wenyuan Rd, Nanjing 210023, Jiangsu, Peoples R China. [Khir, Ragab; Pan, Zhongli; El-Mashad, Hamed] Univ Calif Davis, Dept Biol & Agr Engn, One Shields Ave, Davis, CA 95616 USA. [Khir, Ragab] Suez Canal Univ, Fac Agr, Dept Agr Engn, Ismailia, Egypt. [Pan, Zhongli; Wood, Delilah F.; Berrios, Jose] ARS, Hlthy Processed Foods Res Unit, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. [Tu, Kang] Nanjing Agr Univ, Coll Food Sci & Technol, 1 Weigang St, Nanjing 210095, Jiangsu, Peoples R China. [El-Mashad, Hamed] Mansoura Univ, Dept Agr Engn, Fac Agr, Daqahlia, Egypt. RP Pan, ZL (reprint author), Univ Calif Davis, Dept Biol & Agr Engn, One Shields Ave, Davis, CA 95616 USA.; Pan, ZL (reprint author), ARS, Hlthy Processed Foods Res Unit, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM Zhongli.Pan@ars.usda.gov NR 39 TC 2 Z9 2 U1 11 U2 23 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1935-5130 EI 1935-5149 J9 FOOD BIOPROCESS TECH JI Food Bioprocess Technol. PD JUN PY 2016 VL 9 IS 6 BP 1010 EP 1020 DI 10.1007/s11947-016-1690-5 PG 11 WC Food Science & Technology SC Food Science & Technology GA DK6XD UT WOS:000375068200010 ER PT J AU Lafferty, DJR Hanson-Dorr, KC Prisock, AM Dorr, BS AF Lafferty, Diana J. R. Hanson-Dorr, Katie C. Prisock, Amanda M. Dorr, Brian S. TI Biotic and abiotic impacts of Double-crested cormorant breeding colonies on forested islands in the southeastern United States SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Double-crested Cormorant; Forest health; Human-wildlife conflict; Soil chemistry; Waterbirds; Water quality ID ESCHERICHIA-COLI; PHALACROCORAX-CARBO; RECREATIONAL WATER; PERENNIAL COLONIES; PISCIVOROUS BIRDS; COMMON CORMORANT; DELTA REGION; GREAT-LAKES; MISSISSIPPI; GULLS AB Double-crested Cormorant (Phalacrocorax auritus) numbers have increased in North America, and concomitantly cormorants appear to be expanding their nesting range in the southeastern United States. Because colonial nesting waterbirds can impact water quality, soil chemistry and subsequent vegetation succession patterns, our goal was to assess the extent to which cormorant breeding colonies are influencing the biotic and abiotic attributes of forested islands in the southeastern United States. Our objectives were to (1) compare water quality characteristics in near-shore surface waters around forested islands with and without nesting cormorants during the peak-nesting/fledgling period and post-fledgling period, (2) measure soil chemistry parameters for forested islands with and without nesting cormorants, and (3) compare tree health metrics on forested islands with and without nesting cormorants. Our results indicate that cormorant colonies are not significant contributors to general coliforms or Escherichia coli levels in waters surrounding southern breeding colonies. Cormorants also do not appear to have significant direct effects on water chemistry. We did find that cormorant colonies are affecting soil chemistry. Soil from within the nesting colony was more acidic and had greater concentrations of phosphorous than soils on reference islands. In addition, we found evidence that cormorants are negatively affecting tree health within nesting colonies as evidenced by a greater number of trees of lower vigor class within the nesting colonies compared to reference sites. While cormorants do cause abiotic and biotic changes, these are part of the natural ecological processes that occur following waterbird colonization. Management to reduce unwanted impacts that nesting cormorants are having on forested island habitats should be considered within a framework that allows for natural ecological processes, including changes in soil chemistry and subsequent vegetation succession. Published by Elsevier B.V. C1 [Lafferty, Diana J. R.] Mississippi State Univ, Dept Wildlife Fisheries & Aquaculture, Carnivore Ecol Lab, Mississippi State, MS 39762 USA. [Hanson-Dorr, Katie C.; Dorr, Brian S.] USDA, Wildlife Serv Natl Wildlife Res Ctr, Mississippi Field Stn, POB 6099, Mississippi State, MS 39762 USA. [Prisock, Amanda M.] Weyerhaeuser Co, 29 Tom Rose Rd, Columbus, MS 39704 USA. [Lafferty, Diana J. R.] N Carolina State Univ, David Clark Labs, Program Fisheries Wildlife & Conservat Biol, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. RP Dorr, BS (reprint author), USDA, Wildlife Serv Natl Wildlife Res Ctr, Mississippi Field Stn, POB 6099, Mississippi State, MS 39762 USA. EM djr.lafferty@gmail.com; katie.c.hanson-dorr@aphis.usda.gov; amanda.prisock@weyerhaeuser.com; brian.s.dorr@aphis.usda.gov OI Dorr, Brian/0000-0001-6857-8560; Hanson-Dorr, Katie/0000-0003-4559-938X FU United States Department of Agriculture, Wildlife Services, National Wildlife Research Center FX This research was funded by the United States Department of Agriculture, Wildlife Services, National Wildlife Research Center. We thank Robert Ritchey and Jerry Feist of Unites States Department of Agriculture, Wildlife Services and Paul Fioranelli with the National Wildlife Research Center for logistical and field support. We thank Keith Crouse of Mississippi State University Extension Service Soil Testing Laboratory for conducting soil nutrient analyses and Tara Conkling for advice regarding statistical analysis. NR 56 TC 0 Z9 0 U1 6 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUN 1 PY 2016 VL 369 BP 10 EP 19 DI 10.1016/j.foreco.2016.03.026 PG 10 WC Forestry SC Forestry GA DK8ID UT WOS:000375169500002 ER PT J AU Maynard, JJ Johnson, MG AF Maynard, Jonathan J. Johnson, Mark G. TI Uncoupling the complexity of forest soil variation: Influence of terrain indices, spectral indices, and spatial variability SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Forest soil; Remote sensing; Terrain analysis; Spatial variability; Redundancy analysis; Variation partitioning ID VEGETATION WATER-CONTENT; DIGITAL ELEVATION MODEL; LEAF-AREA INDEX; ETM PLUS DATA; ENVIRONMENTAL CORRELATION; LANDSCAPE; REFLECTANCE; ATTRIBUTES; PREDICTION; DEM AB Growing concern over climate and management induced changes to soil nutrient status has prompted interest in understanding the spatial distribution of forest soil properties. Recent advancements in remotely sensed geospatial technologies are providing an increasing array of data sources (e.g., LiDAR, hyper spectral imagery) relating to forest biophysical properties. While these data sources have the potential to improve spatial predictions of forest soil properties, considerable uncertainty exists regarding which remotely sensed (RS) indices are correlated to soil variability and what underlying pedogenic processes connect them. The main objective of this study was to identify and interpret RS indices that account for soil variability within a 2300 ha forested watershed. Redundancy analysis (RDA) and variation partitioning methods were used to uncouple the complexity of soil-environmental relationships. Thirty-two soil pedons were described, sampled, characterized and analyzed for 22 soil properties within the 0-50 cm soil depth interval. A suite of environmental covariates, comprised of LiDAR derived canopy metrics, land-surface and hydrologic terrain indices, broad-band remotely sensed indices (GeoEye-1), and narrow-band hyper-spectral indices (HyMap), were used as covariates in our RDA models. Principal coordinates of neighbor matrices (PCNM) was used to disentangle the contribution of spatial autocorrelation among sampling locations to the total variance explained by our RDA models. Two groups of soil properties were identified using discriminate analysis of principal components, with each soil property group (SPG) relating to different pedogenic processes occurring with the watershed (SPG1: organic matter metal cycling; SPG2: base-cation cycling). Our results show there was a relatively strong correspondence between soil properties and terrain/spectral indices; with 61% and 81% of the total variance explained by the first four RDA axes for SPG1 and SPG2, respectively. Variation partitioning analysis revealed that both SPG1 and SPG2 were most strongly related to terrain and canopy indices; although spectral indices were also important, especially for SGP2. Variation in the types of RS indices correlated to each SPG results from variation in the degree to which each environmental covariate relates to the pedogenic process (es) driving soil property development. The approach used in this study can help improve our understanding of soil spatial variability through identifying the most significant environmental covariates related to soil variation. Given the growing demands placed upon forest ecosystems (e.g., timber, recreation, carbon sequestration), improved knowledge of soil variability and the factors that affect the soil resource is essential to facilitate more effective forest management. Published by Elsevier B.V. C1 [Maynard, Jonathan J.] New Mexico State Univ, USDA ARS, Jornada Expt Range, POB 30003,MSC 3JER, Las Cruces, NM 88003 USA. [Johnson, Mark G.] US EPA, Natl Hlth & Environm Effects Res Lab, Western Ecol Div, 200 SW 35th St, Corvallis, OR USA. RP Maynard, JJ (reprint author), New Mexico State Univ, USDA ARS, Jornada Expt Range, POB 30003,MSC 3JER, Las Cruces, NM 88003 USA. EM jmaynard@nmsu.edu; johnson.markg@epa.gov NR 100 TC 0 Z9 0 U1 9 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUN 1 PY 2016 VL 369 BP 89 EP 101 DI 10.1016/j.foreco.2016.03.018 PG 13 WC Forestry SC Forestry GA DK8ID UT WOS:000375169500010 ER PT J AU Cal, AJ Sikkema, WD Ponce, MI Franqui-Villanueva, D Riiff, TJ Orts, WJ Pieja, AJ Lee, CC AF Cal, Andrew J. Sikkema, W. Dirk Ponce, Maria I. Franqui-Villanueva, Diana Riiff, Timothy J. Orts, William J. Pieja, Allison J. Lee, Charles C. TI Methanotrophic production of polyhydroxybutyrate-co-hydroxyvalerate with high hydroxyvalerate content SO INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES LA English DT Article DE Polyhydroxyalkanoate; Copolymer; Methanotroph ID PARTICULATE METHANE MONOOXYGENASE; METHYLOSINUS-TRICHOSPORIUM OB3B; POLY-BETA-HYDROXYBUTYRATE; BATCH CULTIVATION; II METHANOTROPH; BACTERIAL POLY(BETA-HYDROXYBUTYRATE-CO-BETA-HYDROXYVALERATE); POTENTIAL APPLICATIONS; POLYHYDROXYALKANOATES; POLY-3-HYDROXYBUTYRATE; POLYESTERS AB Type II methanotrophic bacteria are a promising production platform for PHA biopolymers. These bacteria are known to produce pure poly-3-hydroxybutyrate homopolymer (PHB). We isolated a strain, Methylocystis sp. WERC1, that was capable of producing a wide range of polyhydroxybutyrate-co-hydroxyvalerate copolymers (PHB-co-HV) when co-fed methane and valerate or n-pentanol. The ratio of HB to HV monomer was directly related to the concentration of valeric acid in the PHA accumulation media. We observed increased incorporation of HV and total polymer under copper-free growth conditions. The PHB-co-HV copolymers produced had decreased melting temperatures and crystallinity compared with methanotroph-produced PHB. Published by Elsevier B.V. C1 [Cal, Andrew J.; Sikkema, W. Dirk; Ponce, Maria I.; Franqui-Villanueva, Diana; Riiff, Timothy J.; Orts, William J.; Lee, Charles C.] ARS, USDA, WRRC, Bioprod Res Unit, 800 Buchanan St, Albany, CA 94710 USA. [Pieja, Allison J.] Mango Mat, 490 Lake Pk Ave 16098, Oakland, CA 94610 USA. RP Lee, CC (reprint author), ARS, USDA, WRRC, Bioprod Res Unit, 800 Buchanan St, Albany, CA 94710 USA. EM Charles.Lee@ars.usda.gov FU United States Department of Agriculture CRIS [2030-41000-054-00] FX We would like to thank Lennard Torres for his expert help with DSC analysis and Kelsey Deaton for her efforts in the purification of Methylocystis sp. WRRC1. This work was supported by the United States Department of Agriculture CRIS 2030-41000-054-00. Mention of trade names or commercial products in this report is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 36 TC 2 Z9 2 U1 6 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0141-8130 EI 1879-0003 J9 INT J BIOL MACROMOL JI Int. J. Biol. Macromol. PD JUN PY 2016 VL 87 BP 302 EP 307 DI 10.1016/j.ijbiomac.2016.02.056 PG 6 WC Biochemistry & Molecular Biology; Chemistry, Applied; Polymer Science SC Biochemistry & Molecular Biology; Chemistry; Polymer Science GA DK3IW UT WOS:000374811300039 PM 26920242 ER PT J AU Seo, Y Park, B Hinton, A Yoon, SC Lawrence, KC AF Seo, Youngwook Park, Bosoon Hinton, Arthur, Jr. Yoon, Seung-Chul Lawrence, Kurt C. TI Identification of Staphylococcus species with hyperspectral microscope imaging and classification algorithms SO JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION LA English DT Article DE Hyperspectral microscope imaging; Classification; Staphylococcus; Bacteria; Foodborne pathogen; Serotype; Food safety ID TRANSFORM INFRARED-SPECTROSCOPY; PATHOGEN; BACTERIA; DIFFERENTIATION; ENTERITIDIS; SEROGROUPS AB Hyperspectral microscope imaging method was presented as a rapid and efficient tool to classify gram-positive bacteria species. The datacube (1024 x 1024 x 89) were obtained by hyperspectral microscope imaging system, which provided cell images between 450 and 800 nm wavelengths with 4-nm resolution, resulting in 89 contiguous spectral images that were acquired with an acousto-optic tunable filters (AOTF) hyperspectral imaging platform. Spectral information of bacteria were extracted from region-of-interest (ROI) in the cell, which were approximately between 140 and 380 pixels depending on the size of the cells. Using a Mahalanobis distance algorithm, the outliers beyond 99 % confidence of data were eliminated and classified five species with classification methods including partial least square discriminant analysis (PLS-DA) and support vector machine (SVM) for linear and non-linear classification algorithms to differentiate Staphylococcus species. PLS-DA classified five species with 89.8 % accuracy and 0.87 kappa coefficient; whereas, SVM had much higher classification accuracy of 97.8 % with 0.97 kappa coefficient. To reduce the number of wavelengths for fast data processing, thirty-one spectral bands out of 89 contiguous bands were selected using the correlation of each band. When SVM classification method with selected bands were used, the classification accuracy and kappa coefficient were 93.9 % and 0.92, respectively. C1 [Seo, Youngwook; Park, Bosoon; Hinton, Arthur, Jr.; Yoon, Seung-Chul; Lawrence, Kurt C.] USDA ARS, US Natl Poultry Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. RP Park, B (reprint author), USDA ARS, US Natl Poultry Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. EM bosoon.park@ars.usda.gov NR 34 TC 0 Z9 0 U1 4 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1932-7587 EI 2193-4134 J9 J FOOD MEAS CHARACT JI J. Food Meas. Charact. PD JUN PY 2016 VL 10 IS 2 BP 253 EP 263 DI 10.1007/s11694-015-9301-0 PG 11 WC Food Science & Technology SC Food Science & Technology GA DL2GO UT WOS:000375451800008 ER PT J AU Dhakal, S Chao, KL Qin, JW Kim, M Chan, DN AF Dhakal, Sagar Chao, Kuanglin Qin, Jianwei Kim, Moon Chan, Diane TI Raman spectral imaging for quantitative contaminant evaluation in skim milk powder SO JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION LA English DT Article DE Food safety; Raman spectral imaging; Melamine concentration; Milk powder ID INFANT FORMULA; INFRARED-SPECTROSCOPY; MELAMINE DETECTION; MASS-SPECTROMETRY; RAPID DETECTION; DAIRY-PRODUCTS; ADULTERATION; SEGREGATION; SYSTEM AB This study uses a point-scan Raman spectral imaging system for quantitative detection of melamine in milk powder. A sample depth of 2 mm and corresponding laser intensity of 200 mW were selected after evaluating the penetration of a 785 nm laser through milk powder. Horizontal and vertical spatial resolutions of 0.25 and 0.5 mm were selected for effective detection of melamine particles mixed with milk powder. The selected imaging parameters were used to quantitatively detect melamine concentration in milk powder. Ten different concentrations of melamine in milk (0.005, 0.01, 0.025, 0.05, 0.075, 0.1, 0.25, 0.5, 0.75, and 1 %- w/w) were prepared by mixing each mixture of melamine and milk powder in an acoustic mixer for 15 min, and seven sub-samples of each concentration were imaged. Each sub-sample image was acquired over a 45 mm x 45 mm surface area. Background-corrected Raman spectral images were converted into binary images and an intensity threshold value of 400 was applied to convert melamine pixels into black pixels and milk pixels into white pixels (background). At least one melamine pixel was detected in every sub-sample across all ten concentration levels except for one sub-sample at the 0.005 % concentration. It was found that the method developed in this study can detect melamine particles present at concentrations as low as 0.005 %. A linear relation was established between the detected melamine concentrations and the actual concentrations of the mixtures, with correlation coefficient of 0.99. The results show that the method developed in this study can be used for non-destructive quantitative prediction of melamine in milk powder to minimum concentration level of 0.005 %. C1 [Dhakal, Sagar; Chao, Kuanglin; Qin, Jianwei; Kim, Moon; Chan, Diane] USDA ARS, Beltsville Agr Res Ctr, Environm Microbial & Food Safety Lab, Bldg 303,BARC-East,10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Chao, KL (reprint author), USDA ARS, Beltsville Agr Res Ctr, Environm Microbial & Food Safety Lab, Bldg 303,BARC-East,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM kevin.chao@ars.usda.gov NR 34 TC 2 Z9 2 U1 7 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1932-7587 EI 2193-4134 J9 J FOOD MEAS CHARACT JI J. Food Meas. Charact. PD JUN PY 2016 VL 10 IS 2 BP 374 EP 386 DI 10.1007/s11694-016-9316-1 PG 13 WC Food Science & Technology SC Food Science & Technology GA DL2GO UT WOS:000375451800022 ER PT J AU Gutzwiller, KJ Flather, CH AF Gutzwiller, Kevin J. Flather, Curtis H. TI Avian abundance thresholds, human-altered landscapes, and the challenge of assemblage-level conservation (vol 30, pg 2095, 2015) SO LANDSCAPE ECOLOGY LA English DT Correction C1 [Gutzwiller, Kevin J.] Baylor Univ, Dept Biol, Waco, TX 76798 USA. [Flather, Curtis H.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Gutzwiller, KJ (reprint author), Baylor Univ, Dept Biol, Waco, TX 76798 USA. EM kevin_gutzwiller@baylor.edu NR 1 TC 0 Z9 0 U1 3 U2 6 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD JUN PY 2016 VL 31 IS 5 BP 1155 EP 1155 DI 10.1007/s10980-016-0352-3 PG 1 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DK5LS UT WOS:000374962000016 ER PT J AU Shipitalo, MJ Malone, RW Ma, LW Nolan, BT Kanwar, RS Shaner, DL Pederson, CH AF Shipitalo, Martin J. Malone, Robert W. Ma, Liwang Nolan, Bernard T. Kanwar, Rameshwar S. Shaner, Dale L. Pederson, Carl H. TI Corn stover harvest increases herbicide movement to subsurface drains - Root Zone Water Quality Model simulations SO PEST MANAGEMENT SCIENCE LA English DT Article DE atrazine; metolachlor; modeling; OXA; tile drainage; water quality ID SOIL ORGANIC-CARBON; NO-TILL CORN; ATRAZINE TRANSPORT; RESIDUE REMOVAL; GROUND-WATER; CROP; RZWQM; METOLACHLOR; DEGRADATION; FATE AB BACKGROUND: Crop residue removal for bioenergy production can alter soil hydrologic properties and the movement of agrochemicals to subsurface drains. The Root Zone Water Quality Model (RZWQM), previously calibrated using measured flow and atrazine concentrations in drainage from a 0.4 ha chisel-tilled plot, was used to investigate effects of 50 and 100% corn (Zea mays L.) stover harvest and the accompanying reductions in soil crust hydraulic conductivity and total macroporosity on transport of atrazine, metolachlor and metolachlor oxanilic acid (OXA). RESULTS: The model accurately simulated field-measured metolachlor transport in drainage. A 3 year simulation indicated that 50% residue removal reduced subsurface drainage by 31% and increased atrazine and metolachlor transport in drainage 4-5-fold when surface crust conductivity and macroporosity were reduced by 25%. Based on its measured sorption coefficient, approximately twofold reductions in OXA losses were simulated with residue removal. CONCLUSION: The RZWQM indicated that, if corn stover harvest reduces crust conductivity and soil macroporosity, losses of atrazine and metolachlor in subsurface drainage will increase owing to reduced sorption related to more water moving through fewer macropores. Losses of the metolachlor degradation product OXA will decrease as a result of the more rapid movement of the parent compound into the soil. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. C1 [Shipitalo, Martin J.; Malone, Robert W.] USDA ARS, Ames, IA 50011 USA. [Ma, Liwang; Shaner, Dale L.] USDA ARS, Ft Collins, CO 80522 USA. [Nolan, Bernard T.] US Geol Survey, 959 Natl Ctr, Reston, VA 22092 USA. [Kanwar, Rameshwar S.; Pederson, Carl H.] Iowa State Univ, Ames, IA USA. RP Shipitalo, MJ (reprint author), USDA ARS, Natl Lab Agr & Environm, 2110 Univ Blvd, Ames, IA 50011 USA. EM martin.shipitalo@ars.usda.gov OI Shipitalo, Martin/0000-0003-4775-7345 NR 42 TC 1 Z9 1 U1 3 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD JUN PY 2016 VL 72 IS 6 BP 1124 EP 1132 DI 10.1002/ps.4087 PG 9 WC Agronomy; Entomology SC Agriculture; Entomology GA DL2NK UT WOS:000375471900006 PM 26224526 ER PT J AU Jia, HF Zhao, PC Wang, BJ Tariq, P Zhao, FG Zhao, MZ Wang, QL Yang, TB Fang, JG AF Jia, Haifeng Zhao, Pengcheng Wang, Baoju Tariq, Pervaiz Zhao, Fanggui Zhao, Mizhen Wang, Qinglian Yang, Tianbao Fang, Jinggui TI Overexpression of Polyphenol Oxidase Gene in Strawberry Fruit Delays the Fungus Infection Process SO PLANT MOLECULAR BIOLOGY REPORTER LA English DT Article DE Strawberry; Polyphenol; Polyphenol oxidase; Overexpression; Fungus infection ID PSEUDOMONAS-SYRINGAE; TOMATO; EXPRESSION; RESISTANCE; CLONING; BIOSYNTHESIS; PURIFICATION; PEROXIDASE; HERBIVORY; JASMONATE AB Polyphenols are secondary metabolites widely present in plants which benefit to human health. In the present study we analyzed the changes of polyphenol contents during strawberry fruit development as well as changes of polyphenol oxidase (PPO). The results depicted that the polyphenol content showed a decreasing trend with the fruit development. The pH value impacts the PPO activity, and in strawberry fruit the optimal pH for the PPO activity was 4.5. Meanwhile, PPO activity kept decreasing with the development of the fruit flesh and achenes. The damaged fruit enhanced the PPO activity. We found four PPO genes encoding the PPO in the strawberry that had different expression levels in tissues. The overexpression of the FaPPO1 genes improved the PPO activity in strawberry fruit and delays the fungus infection process. The FaPPO1 gene expression changes had affected the pathogen-related gene expression, such as PAL, SOD, POD, BG, and Chitinase genes. The fruit damage induced the FaPPO1 gene expression, and the abscisic acid and methyl jasmonic were also involved in the regulation of FaPPO1 gene expression. The FaPPO1 and FaPPO2 gene expressions were regulated both by abiotic stresses of low temperature, NaCl, and H2O2 and biotic stresses of powdery mildew and gray mold. Understanding the regulation mechanism of PPO will be helpful and provide meaningful ideas in future for strawberry breeders. C1 [Jia, Haifeng; Zhao, Pengcheng; Wang, Baoju; Tariq, Pervaiz; Zhao, Fanggui; Fang, Jinggui] Nanjing Agr Univ, Hort Coll, Key Lab Genet & Fruit Dev, Nanjing 210095, Jiangsu, Peoples R China. [Zhao, Mizhen; Wang, Qinglian] Jiangsu Acad Sci, Key Lab Mol Biol, Nanjing 210095, Jiangsu, Peoples R China. [Yang, Tianbao] USDA ARS, Beltsville Agr Res Ctr, Key Lab Food Qual, Beltsville, MD 20705 USA. [Fang, Jinggui] Jinggui Fang Nanjing Agr Univ, Nanjing 210095, Jiangsu, Peoples R China. RP Fang, JG (reprint author), Nanjing Agr Univ, Hort Coll, Key Lab Genet & Fruit Dev, Nanjing 210095, Jiangsu, Peoples R China.; Fang, JG (reprint author), Jinggui Fang Nanjing Agr Univ, Nanjing 210095, Jiangsu, Peoples R China. EM Fanggg@njau.edu.cn FU China National Natural Science Fund [31401847]; Jiangsu Natural Science Fund [BK20140707]; China Postdoctoral Science Fund [2014M561663]; Central University basic research and operating expenses of special funding [KJQN201541] FX This work was supported by the China National Natural Science Fund (31401847), Jiangsu Natural Science Fund (BK20140707), China Postdoctoral Science Fund (2014M561663), Central University basic research and operating expenses of special funding ( KJQN201541). NR 45 TC 1 Z9 1 U1 10 U2 25 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0735-9640 EI 1572-9818 J9 PLANT MOL BIOL REP JI Plant Mol. Biol. Rep. PD JUN PY 2016 VL 34 IS 3 BP 592 EP 606 DI 10.1007/s11105-015-0946-y PG 15 WC Biochemical Research Methods; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA DK7DR UT WOS:000375085600005 ER PT J AU Shapulatov, UM Buriev, ZT Ulloa, M Saha, S Devor, EJ Ayubov, MS Norov, TM Shermatov, SE Abdukarimov, A Jenkins, JN Abdurakhmonov, IY AF Shapulatov, U. M. Buriev, Z. T. Ulloa, M. Saha, S. Devor, E. J. Ayubov, M. S. Norov, T. M. Shermatov, S. E. Abdukarimov, A. Jenkins, J. N. Abdurakhmonov, I. Y. TI Characterization of Small RNAs and Their Targets from Fusarium oxysporum Infected and Noninfected Cotton Root Tissues SO PLANT MOLECULAR BIOLOGY REPORTER LA English DT Article DE Cotton; Small RNAs; microRNAs; Fusarium oxysporum (FOV) wilt resistance; Size-directed sRNA cloning ID HEAT-STRESS; MICRORNAS; PLANT; RESISTANCE; MIRNA; PROTEINS; DISEASE; GENES; SET; L. AB Genes for host-plant resistant do exist in cotton (Gossypium spp.) but improvement of cotton cultivars with resistance is difficult due to intensive breeding. Identifying molecular-genetic mechanisms associated with disease resistance can offer a new way to combat a serious threat such as Fusarium oxysporum f. sp. vasinfectum (FOV). Here, we captured and annotated "top-layer" of abundantly and specifically expressed cotton root small RNA (sRNA) including microRNA (miR) sequences during FOV pathogenesis using size-directed and adenylated linker-based sRNA cloning strategy. A total of 4116 candidate sRNA sequences with 16 to 30 nucleotide (nt) length were identified from four complementary DNA (cDNA) libraries of noninfected and FOV race 3-infected roots of susceptible ("11970") versus resistant ("Mebane B-1") cotton genotypes (G. hirsutum L.). The highest numbers of sRNA signatures were those with 19-24 nt long in all libraries, and interestingly, the number of sRNAs substantially increased during FOV infection in a resistant genotype, while it sharply decreased in a susceptible genotype. In BLAST analysis, more than 73 % of sRNAs matched Gossypium (G. arboretum L., G. hirsutum, and G. barbadense L.) ESTs. A small percentage of sRNAs matched A. thaliana (1.68 %), T. cacao (1.26 %), fungal (2 %), and other organism (21.33 %) ESTs. mirBase comparisons showed that 4 % of sRNAs were homologous to previously reported plant miRs, among which we predicted novel cotton Ghr-miR-160 that was not registered in the cotton miR database. These major representative sRNA signatures targeted proteins associated with the key biological processes and molecular functions, explaining the molecular mechanisms of the host defense response during the FOV pathogenesis in cotton. C1 [Shapulatov, U. M.; Buriev, Z. T.; Ayubov, M. S.; Norov, T. M.; Shermatov, S. E.; Abdukarimov, A.; Abdurakhmonov, I. Y.] Acad Sci Uzbek, Ctr Genom & Bioinformat, Minist Agr & Water Resources Republ, Univ Str 2, Tashkent 111215, Qibray Region, Uzbekistan. [Shapulatov, U. M.; Buriev, Z. T.; Ayubov, M. S.; Norov, T. M.; Shermatov, S. E.; Abdukarimov, A.; Abdurakhmonov, I. Y.] Uzpakhtasanoat Assoc, Univ Str 2, Tashkent 111215, Qibray Region, Uzbekistan. [Ulloa, M.] USDA ARS, Plant Stress & Germplasm Dev Res, 3810 4th St, Lubbock, TX 79415 USA. [Saha, S.] USDA ARS, Crop Sci Res Lab, Starkville, MS 39762 USA. [Devor, E. J.] Univ Iowa, Carver Coll Med, Dept Obstet & Gynecol, MERF 3234, Iowa City, IA 52242 USA. RP Abdurakhmonov, IY (reprint author), Acad Sci Uzbek, Ctr Genom & Bioinformat, Minist Agr & Water Resources Republ, Univ Str 2, Tashkent 111215, Qibray Region, Uzbekistan.; Abdurakhmonov, IY (reprint author), Uzpakhtasanoat Assoc, Univ Str 2, Tashkent 111215, Qibray Region, Uzbekistan. EM ibrokhimabdurakhmonov@hotmail.com RI Abdurakhmonov, Ibrokhim/F-5588-2015 OI Abdurakhmonov, Ibrokhim/0000-0001-9563-0686 FU Office of International Research Programs (OIRP) of the United States Department of Agriculture (USDA)-Agricultural Research Service (ARS) [UZB2-31016-TA-09] FX This project was funded by the Office of International Research Programs (OIRP) of the United States Department of Agriculture (USDA)-Agricultural Research Service (ARS) in the frame of USDA-ARS-Former Soviet Union (FSU) cooperation programs with the research grant of UZB2-31016-TA-09. We acknowledge the Academy of sciences of Uzbekistan and US Civilian Research & Development Foundation (CRDF) for project coordination. Mention of trade names or commercial products in this article is solely for providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The US Department of Agriculture is an equal opportunity provider and employer. NR 31 TC 2 Z9 2 U1 10 U2 24 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0735-9640 EI 1572-9818 J9 PLANT MOL BIOL REP JI Plant Mol. Biol. Rep. PD JUN PY 2016 VL 34 IS 3 BP 698 EP 706 DI 10.1007/s11105-015-0945-z PG 9 WC Biochemical Research Methods; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA DK7DR UT WOS:000375085600014 ER PT J AU Kerry, R Goovaerts, P Gimenez, D Oudemans, P Muniz, E AF Kerry, R. Goovaerts, P. Gimenez, D. Oudemans, P. Muniz, E. TI Investigating geostatistical methods to model within-field yield variability of cranberries for potential management zones SO PRECISION AGRICULTURE LA English DT Article DE Cranberries; Geostatistics; Area to point kriging; Factorial kriging; Management zones ID SITE-SPECIFIC MANAGEMENT; PRECISION AGRICULTURE; CROP MANAGEMENT; SOIL; OPPORTUNITY; AREA; VITICULTURE; AGREEMENT; CULTIVAR; QUALITY AB Cranberry harvesting methods give only one yield value per field making characterization of within-field variation, the usual first step in precision farming, difficult. Time-consuming berry count yield and fruit rot estimations are the best "ground truth" indication of yield variation within fields. Correlations and coincidence of binary classifications based on less expensive methods such as enhanced vegetation index (EVI) from imagery, and area to point (AtoP) kriging of useable, poor quality and trash yields were compared with this "ground truth". In general AtoP kriged values gave higher correlations and kappa statistic values with berry counts and fruit rot than EVI. Geostatistical disaggregation of per field yield totals using AtoP kriging with EVI as an external drift (AtoP(KED)) was also investigated. Factorial kriging was used to separate the several scales of variation in "ground truth" and EVI data and determine which ones were most spatially coherent/manageable and which related best to the AtoP kriged data. The spatial trend component of pre-harvest berry counts and AtoP kriging of yields both gave a good initial definition of spatially coherent, relatively permanent management zones. They were related to topography and depth of water table in the soil which are key factors governing cranberry yield. AtoP kriging or AtoP(KED) are recommended for defining management zones as they are less expensive than berry counts. The value of AtoP kriging to precision farmers for other crops to map soils at the farm scale with some imagery and just one bulked soil sample per field or use nutrient levels associated with each polygon of traditional soil survey maps is discussed in the conclusions. C1 [Kerry, R.] Brigham Young Univ, Dept Geog, 690 SWKT, Provo, UT 84602 USA. [Goovaerts, P.] BioMedware Inc, 121 W Washington St,4th Floor TBC, Ann Arbor, MI USA. [Gimenez, D.] Rutgers State Univ, Dept Environm Sci, 14 Coll Farm Rd, New Brunswick, NJ 08903 USA. [Oudemans, P.] Rutgers State Univ, Dept Plant Biol & Pathol, 59 Dudley Rd, New Brunswick, NJ 08903 USA. [Muniz, E.] USDA, Nat Resources Conservat Serv, 220 Davidson Ave, Somerset, NJ USA. RP Kerry, R (reprint author), Brigham Young Univ, Dept Geog, 690 SWKT, Provo, UT 84602 USA. EM ruth_kerry@byu.edu FU USDA-IFAFS [2001-52103-11310]; National Cancer Institute [1R21 ES021570-01A1] FX We are grateful for the reviewer's comments which helped improved this paper markedly. Ocean Spray Cranberries Inc. provided yield data. Funding was provided as part of USDA-IFAFS Grant # 2001-52103-11310. Larisa Pozdnyakova Golovko of RiceTec, Alvin, TX, collected and pre-processed data. Dan A. Sims, Ball State University, calculated EVI values and Richard Shaw USDA-Natural Resources Conservation Service, Somerset, NJ conducted the GPR field surveys. Dr. Goovaerts' work was funded by Grant 1R21 ES021570-01A1 from the National Cancer Institute. The views stated in this publication are those of the authors and do not necessarily represent the official views of the NCI. NR 51 TC 1 Z9 1 U1 7 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-2256 EI 1573-1618 J9 PRECIS AGRIC JI Precis. Agric. PD JUN PY 2016 VL 17 IS 3 SI SI BP 247 EP 273 DI 10.1007/s11119-015-9408-7 PG 27 WC Agriculture, Multidisciplinary SC Agriculture GA DK5MY UT WOS:000374965200001 ER PT J AU Lima, EF Medeiros, RMT Cook, D Lee, ST Kaehler, M Santos-Barbosa, JM Riet-Correa, F AF Lima, Everton F. Medeiros, Rosane Maria T. Cook, Daniel Lee, Stephen T. Kaehler, Miriam Santos-Barbosa, Joyce M. Riet-Correa, Franklin TI Studies in regard to the classification and putative toxicity of Fridericia japurensis (Arrabidaea japurensis) in Brazil SO TOXICON LA English DT Article DE Monofluoroacetate; Sudden death; Fridericia; Poisonous plants; Bignoniaceae ID PALICOUREA-MARCGRAVII; MONOFLUOROACETIC ACID; BIGNONIACEAE; RABBITS; CATTLE; BILABIATA; LIVESTOCK; PLANTS; DEATH AB Numerous plant species worldwide including Palicourea marcgravii (Rubiaceae) and Tanaecium bilabiatum (Bignoniaceae) in Brazil cause acute cardiac failure (sudden death) and are known to contain monofluoroacetate (MFA). Other Bignoniaceae species including Fridericia japurensis (Arrabidaea japurensis) are reported to cause sudden death in livestock in the Brazilian state of Roraima and are suspected to contain MFA due to the similarity of clinical signs. In this study herbarium specimens of Fridericia japurensis and field collections suspected to be F. japurensis were analyzed for MFA, and plant material from the field collections was dosed to rabbits. No MFA was detected in the herbarium specimens authoritatively identified as F. japurensis; however, MFA was detected in the field collections, which were identified as T. bilabiatum. Rabbits dosed orally with T bilabiatum died acutely. Voucher toxic specimens initially described as F. japurensis were incorrectly identified, and the correct botanical name for this plant is T. bilabiatum (Arrabidaea bilabiata). Based on this study we conclude that there are no data to support the toxicity of F. japurensis and that the plant previously reported under this name as causing acute cardiac failure in cattle in Roraima is T. bilabiatum. This research highlights the importance of voucher specimens as part of any toxic plant investigation and corrects the literature regarding these toxic plants. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Lima, Everton F.] Univ Fed Roraima, Dept Vet Med, Boa Vista, Roraima, Brazil. [Medeiros, Rosane Maria T.; Riet-Correa, Franklin] Univ Fed Campina Grande, Vet Hosp, BR-58700000 Patos de Minas, Paraiba, Brazil. [Cook, Daniel; Lee, Stephen T.] ARS, USDA, Poisonous Plant Res Lab, 1150 E 1400 N, Logan, UT 84341 USA. [Kaehler, Miriam] New York Bot Garden, Inst Systemat Bot, 2900 Southern Blv, Bronx, NY 10458 USA. [Santos-Barbosa, Joyce M.] Univ Fed Mato Grosso do Sul, Dept Chem, BR-79070900 Campo Grande, Mato Grosso Do, Brazil. [Riet-Correa, Franklin] Natl Inst Agr Res INIA, CP 70-000, Colonia 39173, Casilla De Corr, Uruguay. RP Riet-Correa, F (reprint author), Univ Fed Campina Grande, Vet Hosp, BR-58700000 Patos de Minas, Paraiba, Brazil. EM franklin.riet@pq.cnpq.br OI Kaehler, Miriam/0000-0001-7851-8764 FU National Institute for Science and Technology for the Control of Plant Poisonings; CNPq [573534/2008-0] FX The authors acknowledge the veterinary practitioners Leonardo Gomes Monteiro Barbosa, Antonio Augusto Ferreira Souza and Silvio Lofego Botelho Neto and the farmers for the information and assistance in this study. This research was supported by the National Institute for Science and Technology for the Control of Plant Poisonings, CNPq, grant 573534/2008-0. NR 26 TC 1 Z9 1 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0041-0101 J9 TOXICON JI Toxicon PD JUN PY 2016 VL 115 BP 22 EP 27 DI 10.1016/j.toxicon.2016.03.001 PG 6 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA DK8FO UT WOS:000375162800003 PM 26945838 ER PT J AU Cano, AM Kohl, K Deleon, S Payton, P Irin, F Saed, M Shah, SA Green, MJ Canas-Carrell, JE AF Cano, Amanda M. Kohl, Kristina Deleon, Sabrina Payton, Paxton Irin, Fahmida Saed, Mohammad Shah, Smit Alkesh Green, Micah J. Canas-Carrell, Jaclyn E. TI Determination of uptake, accumulation, and stress effects in corn (Zea mays L.) grown in single-wall carbon nanotube contaminated soil SO CHEMOSPHERE LA English DT Article DE Microwave-induced heating; Photosynthetic rate; Single-wall carbon nanotube; Drought plant stress ID RADIATION USE EFFICIENCY; ENGINEERED NANOPARTICLES; PLANTS; WATER; MAIZE; NANOMATERIALS; TOXICITY; DROUGHT; YIELD; CELLS AB Single-wall carbon nanotubes (SWNTs) are projected to increase in usage across many industries. Two studies were conducted using Zea L (corn) seeds exposed to SWNT spiked soil for 40 d. In Study 1, corn was exposed to various SWNT concentrations (0, 10, and 100 mg/kg) with different functionalities (non-functionalized, OH-functionalized, or surfactant stabilized). A microwave induced heating method was used to determine SWNTs accumulated mostly in roots (0-24 mu g/g), with minimal accumulation in stems and leaves (2-10 mu g/g) with a limit of detection at 0.1 mu/g. Uptake was not functional group dependent. In Study 2, corn was exposed to 10 mg/kg SWNTs (non-functionalized or COOH-functionalized) under optimally grown or water deficit conditions. Plant physiological stress was determined by the measurement of photosynthetic rate throughout Study 2. No significant differences were seen between control and SWNT treatments. Considering the amount of SWNTs accumulated in corn roots, further studies are needed to address the potential for SWNTs to enter root crop species (i.e., carrots), which could present a significant pathway for human dietary exposure. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Cano, Amanda M.; Kohl, Kristina; Deleon, Sabrina; Canas-Carrell, Jaclyn E.] Texas Tech Univ, Dept Environm Toxicol, Inst Environm & Human Hlth, POB 41163, Lubbock, TX 79409 USA. [Payton, Paxton] USDA ARS, Cropping Syst Res Lab, Plant Stress & Germplasm Dev Unit, Lubbock, TX 79401 USA. [Irin, Fahmida] Texas Tech Univ, Dept Chem Engn, Lubbock, TX 79409 USA. [Saed, Mohammad] Texas Tech Univ, Dept Elect & Comp Engn, Lubbock, TX 79409 USA. [Shah, Smit Alkesh; Green, Micah J.] Texas A&M Univ, Artie McFerrin Dept Chem Engn, College Stn, TX USA. RP Canas-Carrell, JE (reprint author), Texas Tech Univ, Dept Environm Toxicol, Inst Environm & Human Hlth, POB 41163, Lubbock, TX 79409 USA. EM jaclyn.e.canas@ttu.edu FU National Science Foundation (NSF) Chemical, Bioengineering, Environmental, and Transport Systems (CBET) [1133250] FX These studies were funded under the National Science Foundation (NSF) Chemical, Bioengineering, Environmental, and Transport Systems (CBET) (grant # 1133250). Nancy Layland is acknowledged for her help with the caring for the plants of these studies. Jesus Cano is also acknowledged for his help in the mixing of the SWNT soil. NR 44 TC 1 Z9 1 U1 17 U2 44 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0045-6535 EI 1879-1298 J9 CHEMOSPHERE JI Chemosphere PD JUN PY 2016 VL 152 BP 117 EP 122 DI 10.1016/j.chemosphere.2016.02.093 PG 6 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK0QF UT WOS:000374616600015 PM 26966810 ER PT J AU Lacerda, CF Ferreira, JFS Liu, X Suarez, DL AF Lacerda, C. F. Ferreira, J. F. S. Liu, X. Suarez, D. L. TI Evapotranspiration as a Criterion to Estimate Nitrogen Requirement of Maize Under Salt Stress SO JOURNAL OF AGRONOMY AND CROP SCIENCE LA English DT Article DE evapotranspiration; N loss; nutrient demand; photosynthesis; salinity; Zea mays L ID USE EFFICIENCY; WATER-USE; SALINITY; TOLERANCE; NITRATE; YIELD; SOIL; AVAILABILITY; IRRIGATION; GROWTH AB We tested the hypothesis that by reducing the application of N, based on the decrease in evapotranspiration (ET) expected due to increase in soil salinity, it is possible to reduce N loss without causing N deficiency or further yield loss in salt-stressed maize plants. We tested four levels of salinity of irrigation water (S1=0.5; S2=2.5; S3=5.0; and S4=7.5dSm(-1)) and four N rates using outdoor soil columns with five replicates. The N rates were as follows: N1: N recommendation for maize (2.6g per column); N2: 0.3 times the N recommendation (0.78g per column); N3: reduction in N1 based on the decrease in ET caused by salinity; and N4: reduction in N2 based on the decrease in ET caused by salinity. The amounts of N for N3 and N4 were reduced (in relation to N1 and N2) by 7%, 15% and 30% for 2.5, 5.0 and 7.5dSm(-1), respectively. Salinity caused NO3- accumulation in the soil, plant growth inhibition and stomatal closure. The low rates of N (N2 and N4) did not meet the N demand of maize plants, especially for low levels of salinity (control and 2.5dSm(-1)). On the other hand, based on the available growth data, physiological responses and nutritional status, one can conclude that plants under N1 and N3 had the same potential for final yield. For these N rates, reduction in N application according to ET (N3 rate) not only allowed plant growth and maize physiological responses, but also increased N-use efficiency and greatly reduced soil nitrate accumulation compared to N1 rate, at the same levels of salinity. We conclude that reduction in N application, based on reductions in ET, is a good strategy to reduce both the risk of ground water contamination by NO3- leaching and fertilization costs, without causing additional damage to plant development under salt stress. C1 [Lacerda, C. F.] Univ Fed Ceara, Dept Agr Engn, Campus Pici,Bloco 804, BR-60455760 Fortaleza, Ceara, Brazil. [Lacerda, C. F.] CNPq, INCTSal, Campus Pici,Bloco 804, BR-60455760 Fortaleza, Ceara, Brazil. [Ferreira, J. F. S.; Liu, X.; Suarez, D. L.] USDA ARS, US Salin Lab, 4500 Glenwood Dr, Riverside, CA 92501 USA. RP Lacerda, CF (reprint author), Univ Fed Ceara, Dept Agr Engn, Campus Pici,Bloco 804, BR-60455760 Fortaleza, Ceara, Brazil.; Lacerda, CF (reprint author), CNPq, INCTSal, Campus Pici,Bloco 804, BR-60455760 Fortaleza, Ceara, Brazil. EM cfeitosa@ufc.br FU Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq); Instituto Nacional de Ciencia e Tecnologia em Salinidade (INCTSal); Universidade Federal do Ceara, Brazil FX Acknowledgments are due to the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Instituto Nacional de Ciencia e Tecnologia em Salinidade (INCTSal) and Universidade Federal do Ceara, Brazil, for financial support provided to the senior author. Additional support as technical assistance and consumables was provided by US Salinity Laboratory (ARS/USDA). NR 41 TC 1 Z9 1 U1 7 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2250 EI 1439-037X J9 J AGRON CROP SCI JI J. Agron. Crop Sci. PD JUN PY 2016 VL 202 IS 3 BP 192 EP 202 DI 10.1111/jac.12145 PG 11 WC Agronomy SC Agriculture GA DJ6QO UT WOS:000374338200003 ER PT J AU Gesch, RW Royo-Esnal, A Edo-Tena, E Recasens, J Isbell, TA Forcella, F AF Gesch, R. W. Royo-Esnal, A. Edo-Tena, E. Recasens, J. Isbell, T. A. Forcella, F. TI Growth environment but not seed position on the parent plant affect seed germination of two Thlaspi arvense L. populations SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Thlaspi arvense; Pennycress; Germination; Seed dormancy; Seed oil ID FATTY-ACIDS; CORN-BELT; EMERGENCE; CROP; TEMPERATURE; DORMANCY; SOIL; OIL; CRUCIFERAE; BIODIESEL AB Thlaspi arvense L is a common weed found in most temperate regions throughout the world that also shows excellent potential for domestication as an oilseed crop. The complexity of T. arvense seed dormancy presently makes it difficult to predict its emergence and manage it as an oilseed crop. Therefore, a better understanding of factors controlling seed dormancy will help develop strategies for managing its weediness and facilitate development of crop genotypes. T. arvense has semi-indeterminate growth and seeds developed at the base of the inflorescence (basal) generally mature sooner than those near the top (apical). However, little is known about the maternal influences on the level of dormancy and germination of basal and apical seeds. To study this, two T. arvense populations, one from Spain and another from USA, were both grown at field sites in Almenar, Spain and Morris, Minnesota, USA during the same growing season (autumn 2012-summer of 2013). Basal and apical seeds collected at maturity were analyzed for mass, total protein and carbon content, oil content, and fatty acid profiles. Under controlled environment conditions, seed germination was measured at 5, 8, 11, 14, 17, and 20 degrees C and germination rate estimated at 8 C. Seed position on the inflorescence did not affect germination consistently nor were there clear differences in seed attributes between positions. However, seeds of both populations produced at Almenar had lower germination and were consistently larger and contained higher oil and carbon content than seeds produced at Morris. Results indicate that environmental influences at each growth location played a larger role in influencing dormancy and germination of seeds produced than did the seed attributes measured or position of seeds on the inflorescence. Published by Elsevier B.V. C1 [Gesch, R. W.; Forcella, F.] USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. [Royo-Esnal, A.; Edo-Tena, E.; Recasens, J.] Univ Lleida, Dept Hortofructicultura Bot & Jardineria, Agrotecnio, Lleida 25198, Spain. [Isbell, T. A.] USDA ARS, Natl Ctr Agr Utilizat Res, Peori, IL USA. RP Gesch, RW (reprint author), USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. EM russ.gesch@ars.usda.gov RI Recasens, Jordi/B-3970-2011; Royo-Esnal, Aritz/B-4174-2011 OI Recasens, Jordi/0000-0002-8799-9958; Royo-Esnal, Aritz/0000-0003-2534-8372 FU Sociedad Espanola de Malherbologia (SEMh); [AGL2010-22084-C02-01] FX The authors would like to thank Mr. Joe Boots for helping to maintain plots in Morris, Minnesota and collect and process seeds. The authors also thank Ms. Linda Manthey of the USDA-ARS National Center for Agriculture Utilization Lab in Peoria, Illinois for analyzing oil content and fatty acid profiles of the seed oil. The experiment carried out in Almenar, Spain was supported by the Spanish project AGL2010-22084-C02-01 and by a grant from the Sociedad Espanola de Malherbologia (SEMh) in 2013. We would also like to thank Ines Olalla for the germination experiment assistance and Ana Isabal and Joel Torra for the field assistance during 2012-13. NR 33 TC 3 Z9 3 U1 5 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD JUN PY 2016 VL 84 BP 241 EP 247 DI 10.1016/j.indcrop.2016.02.006 PG 7 WC Agricultural Engineering; Agronomy SC Agriculture GA DI5KO UT WOS:000373538000029 ER PT J AU Zhuang, H Bowker, B AF Zhuang, Hong Bowker, Brian TI Effect of marination on lightness of broiler breast fillets varies with raw meat color attributes SO LWT-FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Marination; Breast meat; Chicken; Pale; CIELAB L* ID CHICKEN BREAST; PALE; PH; SOFT AB Marination effect on meat color L* values was evaluated in chicken breast fillets with different color attributes. Samples were marinated in a vacuum tumbler. Color was measured on the fillet dorsal side at 4 h (pre-marination) and 48 h postmortem (PM). There was no difference (P < 0.05) in L* values between non-marinated 4 h and 48 h pale samples; however, L* values in marinated pale fillets at 48 h PM decreased by more than 5 units. L* values in non-marinated normal and dark fillets increased by more than 5 units at 48 h PM. But L* values of marinated normal fillets were lower than non-marinated controls by more than 3 units and there was no difference in L* values between marinated samples and controls in dark fillets after 48 h PM. Data demonstrate that marination reduces lightness in pale breast fillets, prevents lightness in normal fillets from increasing during PM aging, and has no effect on lightness in dark fillets. (c) 2016 Published by Elsevier Ltd. C1 [Zhuang, Hong; Bowker, Brian] ARS, US Natl Poultry Res Ctr, USDA, 950 Coll Stn Rd, Athens, GA 30605 USA. RP Zhuang, H (reprint author), ARS, US Natl Poultry Res Ctr, USDA, 950 Coll Stn Rd, Athens, GA 30605 USA. EM hong.zhuang@ars.usda.gov NR 11 TC 0 Z9 0 U1 4 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0023-6438 EI 1096-1127 J9 LWT-FOOD SCI TECHNOL JI LWT-Food Sci. Technol. PD JUN PY 2016 VL 69 BP 233 EP 235 DI 10.1016/j.lwt.2016.01.054 PG 3 WC Food Science & Technology SC Food Science & Technology GA DI5LC UT WOS:000373539500033 ER PT J AU McKeith, RO King, DA Grayson, AL Shackelford, SD Gehring, KB Savell, JW Wheeler, TL AF McKeith, Russell O. King, D. Andy Grayson, Adria L. Shackelford, Steven D. Gehring, Kerri B. Savell, Jeffrey W. Wheeler, Tommy L. TI Mitochondrial abundance and efficiency contribute to lean color of dark cutting beef SO MEAT SCIENCE LA English DT Article DE Dark cutting beef; Color; Color stability; Electron transport; Mitochondria ID PORCINE LONGISSIMUS MUSCLE; HIGHER OXIDATIVE STRESS; RESIDUAL FEED-INTAKE; SINGLE GENETIC LINE; PROTEIN EXPRESSION; COMPLEX ACTIVITY; BROILERS; GLYCOLYSIS; QUALITY; OXYGEN AB Beef carcasses exhibiting four levels of dark cutting severity (DCS): Severe, Moderate, Mild, and Shady were compared to Control carcasses to investigate biochemical traits contributing to the dark cutting condition. Color attributes of Longissimus lumborum (LL) were measured after grading and during simulated retail display. Mitochondrial abundance and efficiency, bloomed oxymyoglobin, reducing ability, glycolytic potential, myoglobin concentration, and protein solubility and oxidation were determined. Glycolytic potential and lactate concentrations decreased (P < 0.05) as DCS increased. Residual glycogen was greater (P < 0.05) in steaks from Control carcasses compared to DCS classes. Generally, as DCS increased, LL steaks were darker and less red in color (P < 0.05). Increased (P < 0.05) oxygen consumption and reducing ability coincided with greater myoglobin concentration and greater abundance of less efficient mitochondria as DCS increased (P < 0.05). These data suggest the dark cutting condition is associated with greater oxidative metabolism coupled with less efficient mitochondria resulting in depletion of glycogen during stress. Published by Elsevier Ltd. C1 [McKeith, Russell O.; Grayson, Adria L.; Gehring, Kerri B.; Savell, Jeffrey W.] Texas A&M Univ, Dept Anim Sci, Texas A&M AgriLife Res, College Stn, TX 77843 USA. [King, D. Andy; Shackelford, Steven D.; Wheeler, Tommy L.] USDA ARS, Roman L Hruska US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. [McKeith, Russell O.] Coll Sequoias Tulare Coll Ctr, Div Agr, Tulare, CA 93274 USA. [Grayson, Adria L.] Sadlers Smokehouse, Henderson, TX 75652 USA. RP King, DA (reprint author), USDA ARS, Roman L Hruska US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. EM andy.king@ars.gov RI Savell, Jeffrey/C-2434-2008 OI Savell, Jeffrey/0000-0003-0354-1286 NR 41 TC 2 Z9 2 U1 9 U2 14 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0309-1740 EI 1873-4138 J9 MEAT SCI JI Meat Sci. PD JUN PY 2016 VL 116 BP 165 EP 173 DI 10.1016/j.meatsci.2016.01.016 PG 9 WC Food Science & Technology SC Food Science & Technology GA DH7AE UT WOS:000372942500021 PM 26890392 ER PT J AU Fang, L Hain, CR Zhan, XW Anderson, MC AF Fang, Li Hain, Christopher R. Zhan, Xiwu Anderson, Martha C. TI An inter-comparison of soil moisture data products from satellite remote sensing and a land surface model SO INTERNATIONAL JOURNAL OF APPLIED EARTH OBSERVATION AND GEOINFORMATION LA English DT Article DE Soil moisture product; Validation; Active and passive microwave; Thermal infrared remote sensing ID FRACTIONAL VEGETATION COVER; DATA ASSIMILATION SYSTEM; INFORMATION-SYSTEM; ENERGY FLUXES; SENSED DATA; PART I; TEMPERATURE; RETRIEVAL; ASCAT; IMPLEMENTATION AB Significant advances have been achieved in generating soil moisture (SM) products from satellite remote sensing and/or land surface modeling with reasonably good accuracy in recent years. However, the discrepancies among the different SM data products can be considerably large, which hampers their usage in various applications. The bias of one SM product from another is well recognized in the literature. Bias estimation and spatial correction methods have been documented for assimilating satellite SM product into land surface and hydrologic models. Nevertheless, understanding the characteristics of each of these SM data products is required for many applications where the most accurate data products are desirable. This study inter-compares five SM data products from three different sources with each other, and evaluates them against in situ SM measurements over 14-year period from 2000 to 2013. Specifically, three microwave (MW) satellite based data sets provided by ESA's Climate Change Initiative (CCI) (CCI-merged, -active and -passive products), one thermal infrared (TIR) satellite based product (ALEXI), and the Noah land surface model (LSM) simulations. The in-situ SM measurements are collected from the North American Soil Moisture Database (NASMD), which involves more than 600 ground sites from a variety of networks. They are used to evaluate the accuracies of these five SM data products. In general, each of the five SM products is capable of capturing the dry/wet patterns over the study period. However, the absolute SM values among the five products vary significantly. SM simulations from Noah LSM are more stable relative to the satellite-based products. All TIR and MW satellite based products are relatively noisier than the Noah LSM simulations. Even though MW satellite based SM retrievals have been predominantly used in the past years, SM retrievals of the ALEXI model based on TIR satellite observations demonstrate skills equivalent to all the MW satellite retrievals and even slightly better over certain regions. Compared to the individual active and passive MW products, the merged CCI product exhibits higher anomaly correlation with both Noah LSM simulations and in-situ SM measurements. (C) 2015 Elsevier B.V. All rights reserved. C1 [Fang, Li; Hain, Christopher R.] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, 5825 Univ Res Court, College Pk, MD 20740 USA. [Fang, Li; Hain, Christopher R.; Zhan, Xiwu] NOAA, NESDIS, STAR, 5830 Univ Res Court, College Pk, MD 20740 USA. [Anderson, Martha C.] ARS, USDA, Hydrol & Remote Sensing Lab, 104 Bldg 007,BARC West, Beltsville, MD 20705 USA. RP Fang, L (reprint author), Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, 5825 Univ Res Court, College Pk, MD 20740 USA. EM li.fang@noaa.gov RI Zhan, Xiwu/F-5487-2010; Anderson, Martha/C-1720-2015 OI Anderson, Martha/0000-0003-0748-5525 FU NOAA JPSS Risk Reduction and Proving Ground Program(Enhancing Agricultural Drought Monitoring Using NPP/JPSS Land EDRs for NIDIS); NOAA-NESDIS PSDI Program (GET-D; GOES Evapotranspiration (ET) and Drought Product System) FX This work is supported by the NOAA JPSS Risk Reduction and Proving Ground Program(Enhancing Agricultural Drought Monitoring Using NPP/JPSS Land EDRs for NIDIS) and the NOAA-NESDIS PSDI Program (GET-D; GOES Evapotranspiration (ET) and Drought Product System). NR 72 TC 0 Z9 0 U1 10 U2 45 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0303-2434 J9 INT J APPL EARTH OBS JI Int. J. Appl. Earth Obs. Geoinf. PD JUN PY 2016 VL 48 BP 37 EP 50 DI 10.1016/j.jag.2015.10.006 PG 14 WC Remote Sensing SC Remote Sensing GA DH3JC UT WOS:000372682300005 ER PT J AU Sakai, T Iizumi, T Okada, M Nishimori, M Grunwald, T Prueger, J Cescatti, A Korres, W Schmidt, M Carrara, A Loubet, B Ceschia, E AF Sakai, Toru Iizumi, Toshichika Okada, Masashi Nishimori, Motoki Gruenwald, Thomas Prueger, John Cescatti, Alessandro Korres, Wolfgang Schmidt, Marius Carrara, Arnaud Loubet, Benjamin Ceschia, Eric TI Varying applicability of four different satellite-derived soil moisture products to global gridded crop model evaluation SO INTERNATIONAL JOURNAL OF APPLIED EARTH OBSERVATION AND GEOINFORMATION LA English DT Article DE Soil moisture; Satellite remote sensing; Flux tower observation; Intercomparison; Cropland ID RICE PADDY FIELD; TIBETAN PLATEAU; ENERGY-BALANCE; RETRIEVALS; NETWORK; SURFACE; YIELDS; VARIABILITY; VALIDATION; ECOSYSTEM AB Satellite-derived daily surface soil moisture products have been increasingly available, but their applicability to global gridded crop model (GGCM) evaluation is unclear. This study compares four different soil moisture products with the flux tower site observation at 18 cropland sites across the world where either of maize, soybean, rice and wheat is grown. These products include the first and second versions of Climate Change Initiative Soil Moisture (CCISM-1 and CCISM-2) datasets distributed by the European Space Agency and two different AMSR-E (Advanced Microwave Scanning Radiometer-Earth Observing System)-derived soil moisture datasets, separately provided by the Japan Aerospace Exploration Agency (AMSRE-J) and U.S. National Aeronautics and Space Administration (AMSRE-N). The comparison demonstrates varying reliability of these products in representing major characteristics of temporal pattern of cropland soil moisture by product and crop. Possible reasons for the varying reliability include the differences in sensors, algorithms, bands and criteria used when estimating soil moisture. Both the CCISM-1 and CCISM-2 products appear the most reliable for soybean- and wheat-growing area. However, the percentage of valid data of these products is always lower than other products due to relatively strict criteria when merging data derived from multiple sources, although the CCISM-2 product has much more data with valid retrievals than the CCISM-1 product. The reliability of the AMSRE-J product is the highest for maize- and rice-growing areas and comparable to or slightly lower than the CCISM products for soybean and wheat-growing areas. The AMSRE-N is the least reliable in most location-crop combinations. The reliability of the products for rice-growing area is far lower than that of other upland crops likely due to the extensive use of irrigation and patch distribution of rice paddy in the area examined here. We conclude that the CCISM-1, CCISM-2 and AMSRE-J products are applicable to GGCM evaluation, while the AMSRE-N product is not. However, we encourage users to integrate these products with in situ soil moisture data especially when GGCMs simulations for rice are evaluated. (C) 2015 Elsevier B.V. All rights reserved. C1 [Sakai, Toru; Iizumi, Toshichika; Okada, Masashi; Nishimori, Motoki] Natl Inst Agroenvironm Sci, Agrometeorol Div, 3-1-3 Kannondai, Tsukuba, Ibaraki 3048604, Japan. [Gruenwald, Thomas] Tech Univ Dresden, Inst Hydrol & Meteorol, Chair Meteorol, D-01062 Dresden, Germany. [Prueger, John] ARS, Natl Lab Agr & Environm, USDA, NLAE, 2110 Univ Blvd, Ames, IA 50011 USA. [Cescatti, Alessandro] Commiss European Communities, Joint Res Ctr, Inst Environm & Sustainabil, I-21020 Ispra, Italy. [Korres, Wolfgang] Univ Cologne, Inst Geog, D-50923 Cologne, Germany. [Schmidt, Marius] Inst Bio & Geosci, Agrosphere Inst IBG 3, D-52425 Julich, Germany. [Carrara, Arnaud] Fdn Ctr Estudios Ambientales Mediterraneo CEAM, Charles Robert Darwin,14,Parque Tecnol, Paterna 46980, Spain. [Loubet, Benjamin] INRA, UMR, AgroParisTech, ECOSYS, F-78850 Thiverval Grignon, France. [Ceschia, Eric] UPS, CNES, IRD, CNRS,CESBIO, 18 Ave Edouard Belin, F-931401 Toulouse 9, France. RP Sakai, T (reprint author), Natl Inst Agroenvironm Sci, Agrometeorol Div, 3-1-3 Kannondai, Tsukuba, Ibaraki 3048604, Japan. RI Schmidt, Marius/I-8746-2012; ceschia, eric/P-5054-2014; OI ceschia, eric/0000-0001-5941-752X; Schmidt, Marius/0000-0001-5292-7092 FU Environment Research and Technology Development Fund of the Ministry of the Environment, Japan [S-10-2, S-14-3]; CARBORED-II [CGL2010-22193-C04-01]; Deutsche Forschungsgemeinschaft (DFG) [SFB/TR 32] FX We thank A. Kumai at University of Tsukuba for her support in collecting data at FluxNet sites. Acknowledgements are extended to Dr. Dario Papale at University of Tuscia who provided the site observation data used in this study. T. Iizumi, M. Okada and M. Nishimori were supported by the Environment Research and Technology Development Fund (S-10-2 and S-14-3) of the Ministry of the Environment, Japan. A. Carrara was supported by the CARBORED-II (CGL2010-22193-C04-01). W. Korres and M. Schmidt gratefully acknowledge financial support by the SFB/TR 32 "Patterns in Soil Vegetation-Atmosphere Systems: Monitoring, Modelling, and Data Assimilation" funded by the Deutsche Forschungsgemeinschaft (DFG). NR 49 TC 0 Z9 0 U1 3 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0303-2434 J9 INT J APPL EARTH OBS JI Int. J. Appl. Earth Obs. Geoinf. PD JUN PY 2016 VL 48 BP 51 EP 60 DI 10.1016/j.jag.2015.09.011 PG 10 WC Remote Sensing SC Remote Sensing GA DH3JC UT WOS:000372682300006 ER PT J AU Schumacher, BA Shimada, K Liston, J Maltese, A AF Schumacher, Bruce A. Shimada, Kenshu Liston, Jeff Maltese, Anthony TI Highly specialized suspension-feeding bony fish Rhinconichthys (Actinopterygii: Pachycormiformes) from the mid-Cretaceous of the United States, England, and Japan SO CRETACEOUS RESEARCH LA English DT Article DE Cenomanian; Osteichthyes; Pachycormid; Phylogeny; Planktivory; Turonian ID PREY CAPTURE; ELASMOBRANCHS; MECHANISMS; EVOLUTION; ORIGIN; POINT AB We re-define the Cretaceous bony fish genus Rhinconichthys by re-describing the type species, R. taylori, and defining two new species; R. purgatorensis sp. nov. from the lowermost Carlile Shale (middle Turonian), southeastern Colorado, United States, and R. uyenoi sp. nov. from the Mikasa Formation (Cenomanian), Middle Yezo Group, Hokkaido, Japan. Rhinconichthys purgatoirensis sp. nov. is designated on a newly discovered specimen consisting of a nearly complete skull with pectoral elements. Only known previously by two Cenomanian age specimens from England and Japan, the North American specimen significantly extends the geographic and stratigraphic range of Rhinconichthys. The skull of Rhinconichthys is elongate, including an expansive gill basket, and estimated maximum body length ranges between 2.0 and 2.7 m. Rhinconichthys was likely an obligate suspension-feeder due to its derived cranial morphology, characterized by a remarkably large and elongate hyomandibula. The hyomandibula mechanically acts as a lever to thrust the jaw articulation and hyoid arch both ventrally and anterolaterally during protraction, thus creating a massive buccal space to maximize filtering of planktonic prey items. Cladistic analysis supports a monophyly of suspension-feeding pachycormids including Rhinconichthys, but further resolution within this Glade will require more information through additional fossil specimens. Published by Elsevier Ltd. C1 [Schumacher, Bruce A.] US Forest Serv, USDA, La Junta, CO 81050 USA. [Schumacher, Bruce A.] Denver Museum Nat & Sci, Denver, CO 80205 USA. [Shimada, Kenshu] Depaul Univ, Dept Environm Sci & Studies, 2325 North Clifton Ave, Chicago, IL 60614 USA. [Shimada, Kenshu] Depaul Univ, Dept Biol Sci, 2325 North Clifton Ave, Chicago, IL 60614 USA. [Shimada, Kenshu] Ft Hays State Univ, Sternberg Museum Nat Hist, Hays, KS 67601 USA. [Liston, Jeff] Yunnan Univ, Yunnan Key Lab Palaeobiol, Kunming 650091, Yunnan, Peoples R China. [Liston, Jeff] Natl Museum Scotland, Dept Nat Sci, Chambers St, Edinburgh EH1 1JF, Midlothian, Scotland. [Liston, Jeff] Univ Bristol, Sch Earth Sci, Wills Mem Bldg,Queens Rd, Bristol BS8 1RJ, Avon, England. [Liston, Jeff] Univ Glasgow, Coll Med Vet & Life Sci, Inst Biodivers Anim Hlth & Comparat Med, Univ Ave, Glasgow G12 8QQ, Lanark, Scotland. [Maltese, Anthony] Rocky Mt Dinosaur Resource Ctr, Woodland Pk, CO 80863 USA. RP Schumacher, BA (reprint author), US Forest Serv, USDA, La Junta, CO 81050 USA. EM baschumacher@fs.fed.us NR 41 TC 1 Z9 1 U1 5 U2 14 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0195-6671 EI 1095-998X J9 CRETACEOUS RES JI Cretac. Res. PD JUN PY 2016 VL 61 BP 71 EP 85 DI 10.1016/j.cretres.2015.12.017 PG 15 WC Geology; Paleontology SC Geology; Paleontology GA DG2ZJ UT WOS:000371939000008 ER PT J AU Jabro, JD Iversen, WM Stevens, WB Evans, RG Mikha, MM Allen, BL AF Jabro, Jay D. Iversen, William M. Stevens, William B. Evans, Robert G. Mikha, Maysoon M. Allen, Brett L. TI Physical and hydraulic properties of a sandy loam soil under zero, shallow and deep tillage practices SO SOIL & TILLAGE RESEARCH LA English DT Article DE Zero tillage; Bulk density; Infiltration rate; Hydraulic conductivity ID WATER-CONTENT; BULK-DENSITY; ROOT-GROWTH; NO-TILLAGE; CONSERVATION TILLAGE; STRIP TILLAGE; CROP YIELD; SUGAR-BEET; SYSTEMS; MACROPOROSITY AB The effect of tillage depth on physical properties in sandy soils under sugarbeet (Beta vulgaris L) is not well understood and documented. A 4-yr study was conducted to investigate the effect of zero tillage (ZT), shallow tillage (ST), and deep tillage (DT) practices on soil bulk density (BD), saturated hydraulic conductivity (Ks), and moisture content (MC) in a sandy loam. Undisturbed soil cores were collected at 0 to 10,10- to 20, 20- to 30, and 30- to 40 cm depths to measure BD and MC under each tillage practice. Soil Ks for the surface and subsurface layers were measured using a single head pressure ring infiltrometer and a constant head well permeameter, respectively. Soil BD was not significantly influenced by tillage in the 0- to 10, 10- to 20, 20- to 30, and 30- to 40 cm layers when averaged for four years. When averaged across the 0- to 40 cm depth, soil BD did not differ significantly among three tillages in 2008, 2009 and 2010; however, BD was significantly lower in DT (1.50 Mg m(-3)) than in ZT (1.61 Mg m(-3)) and ST (1.59 Mg m(-3)) in 2011. Soil BD averaged over all years and layers resulted in nonsignificant differences among three tillage practices. Soil Ks did not differ significantly among three tillage practices in 2008, 2009, and 2011 for the 0- to 40 cm depth. Similarly, soil Ks averaged across four years and four layers was not affected by tillage. In 2010, Ks was significantly greater in ST (27.60 mm h(-1)) than in ZT (17.21 mm h(-1)) and DT (12.46 mm h(-1)). Soil MC was not significantly influenced by tillage in any of the four depths when averaged across four years and when also averaged across four depths for each year. Across four years and four depths, averaged MC was not influenced by tillage. Large variations in Ks among ZT, ST and DT were likely caused by soil variability among replications within each tillage treatment. We concluded that tillage did not significantly affect BD, Ks and soil MC most likely due to the unchanging total porosity in sandy loam soil regardless of tillage type. (C) 2016 Published by Elsevier B.V. C1 [Jabro, Jay D.; Iversen, William M.; Stevens, William B.; Evans, Robert G.; Allen, Brett L.] ARS, Northern Plains Agr Res Lab, USDA, 1500 N Cent Ave, Sidney, MT 59270 USA. [Mikha, Maysoon M.] Cent Plains Resources Management Res, 40335 Cty Rd GG, Akron, CO 80720 USA. RP Jabro, JD (reprint author), ARS, Northern Plains Agr Res Lab, USDA, 1500 N Cent Ave, Sidney, MT 59270 USA. EM jay.jabro@ars.usda.gov NR 33 TC 0 Z9 1 U1 15 U2 50 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-1987 EI 1879-3444 J9 SOIL TILL RES JI Soil Tillage Res. PD JUN PY 2016 VL 159 BP 67 EP 72 DI 10.1016/j.still.2016.02.002 PG 6 WC Soil Science SC Agriculture GA DG2YS UT WOS:000371937300008 ER PT J AU Otoni, CG Avena-Bustillos, RJ Olsen, CW Bilbao-Sainz, C McHugh, TH AF Otoni, Caio G. Avena-Bustillos, Roberto J. Olsen, Carl W. Bilbao-Sainz, Cristina McHugh, Tara H. TI Mechanical and water barrier properties of isolated soy protein composite edible films as affected by carvacrol and cinnamaldehyde micro and nanoemulsions SO FOOD HYDROCOLLOIDS LA English DT Article DE Biopolymer; Edible film; Soy protein; Active packaging; Essential oil; Nanotechnology ID PLANT ESSENTIAL OILS; ESCHERICHIA-COLI O157-H7; LIPID EMULSION FILMS; VAPOR PERMEABILITY; ANTIMICROBIAL PROPERTIES; MOISTURE DIFFUSIVITY; ANTIBACTERIAL ACTIVITIES; PHYSICAL-PROPERTIES; DELIVERY-SYSTEMS; NANO-EMULSIONS AB Edible films may be used in food packaging, for which they must deliver good barrier and mechanical properties. Films based on proteins have good gas barrier and mechanical properties, but poor water barrier properties. Films made from lipids have good water barrier properties, but poor mechanical properties. Protein and lipids were then combined to form composite films, and the particle size of the lipid phase was reduced to evaluate its effect on the mechanical and barrier properties. Micro and nanodroplets of Acetem and Tween 60 were added into an isolated soy protein (ISP) solution. Oil-inwater droplets were formed by direct emulsification at 1300 rpm for 30 min (microdroplets) or 5 h (nanodroplets), dispersed into the ISP solution, and cast into films. Emulsified films showed reduced strength and increased elongation, indicating a plasticizing effect of emulsions. The water barrier properties were either unchanged or slightly improved by the addition of hydrophobic compounds. Reducing droplet size improved the barrier properties as surface area of the lipid increased. Carvacrol and cinnamaldehyde were also added, and they either improved or unaffected the mechanical and barrier properties of ISP films. Addition of emulsions and reduction of droplet size generally decreased total pore volume in the films as well as water vapor permeation, indicating that the microvoid model suitably explains the water barrier properties of ISP films. This work further clarifies possible differences in barrier properties by examining water diffusivity and adsorption/desorption kinetics. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Otoni, Caio G.; Avena-Bustillos, Roberto J.; Olsen, Carl W.; Bilbao-Sainz, Cristina; McHugh, Tara H.] ARS, Hlth Proc Foods Res, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. RP Otoni, CG (reprint author), Embrapa Instrumentat, Natl Nanotechnol Lab Agribusiness, 1452 XV Novembro St, BR-13560979 Sao Carlos, SP, Brazil. EM caiogomide@gmail.com; roberto.avena@ars.usda.gov; carl.olsen@ars.usda.gov; cristina.bilbao@ars.usda.gov; tara.mchugh@ars.usda.gov RI Otoni, Caio/F-6743-2012 OI Otoni, Caio/0000-0001-6734-7381 NR 50 TC 6 Z9 6 U1 22 U2 82 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0268-005X EI 1873-7137 J9 FOOD HYDROCOLLOID JI Food Hydrocolloids PD JUN PY 2016 VL 57 BP 72 EP 79 DI 10.1016/j.foodhyd.2016.01.012 PG 8 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DG1FZ UT WOS:000371812600009 ER PT J AU Fisher, KD Bratcher, CL Jin, TZ Bilgili, SF Owsley, WF Wang, LX AF Fisher, Kimberly D. Bratcher, Christy L. Jin, Tony Z. Bilgili, Sacit F. Owsley, Walter F. Wang, Luxin TI Evaluation of a novel antimicrobial solution and its potential for control Escherichia coli O157:H7, non-O157:H7 shiga toxin-producing E. coli, Salmonella spp., and Listeria monocytogenes on beef SO FOOD CONTROL LA English DT Article DE Antimicrobial solution; Foodborne pathogens; Marinades; Meat ID CAMPYLOBACTER-JEJUNI; INACTIVATION; COMPONENTS; SURVIVAL; COATINGS; FOOD; CHITOSAN; INNOCUA; GROWTH; MEAT AB The goal of this study was to evaluate the efficacy of a novel antimicrobial solution made with chitosan, lauric arginate ester, and organic acids on Escherichia coli O157:H7, Salmonella spp., Listeria monocytogenes, and non-O157 shiga toxin-producing E. coli cocktails and to test its potential to be used as a marinade for raw beef. Fresh beef top round steaks were surface-inoculated with the pathogen cocktails at approximately 2.5 or 4.5 Log CFU/cm(2), marinated with the antimicrobial solution (AMS), and then stored at 4 degrees C for 6, 24, and 48 h. Three commercially available marinades were used for comparison. Results revealed that AMS had the most antimicrobial effect regardless of the type or inoculation level of pathogens (P < 0.05). After 6 h, the AMS marination reduced all pathogens to levels below the limit of detection (<1 Log CFU/cm(2)), resulting in a 3.5 Log CFU/cm(2) reduction. When AMS was diluted with autoclaved distilled water by 5 times (AMS 1:5) or 10 times (AMS 1:10), its antimicrobial efficacy was impacted by marination time, the inoculated pathogens, and the inoculation levels. This study demonstrates that the developed antimicrobial solution has a great potential to be used during marination by consumers to ensure better food safety. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Fisher, Kimberly D.; Bratcher, Christy L.; Owsley, Walter F.; Wang, Luxin] Auburn Univ, Dept Anim Sci, Auburn, AL 36849 USA. [Jin, Tony Z.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Bilgili, Sacit F.] Auburn Univ, Dept Poultry Sci, Auburn, AL 36849 USA. RP Wang, LX (reprint author), 210 Upchurch Hall, Auburn, AL 36849 USA. EM lzw0022@auburn.edu RI Fiori, Alex/H-6065-2016 NR 17 TC 0 Z9 0 U1 5 U2 53 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD JUN PY 2016 VL 64 BP 196 EP 201 DI 10.1016/j.foodcont.2015.12.007 PG 6 WC Food Science & Technology SC Food Science & Technology GA DF2QN UT WOS:000371189000028 ER PT J AU Yi, J Fan, YT Yokoyama, W Zhang, YZ Zhao, LQ AF Yi, Jiang Fan, Yuting Yokoyama, Wallace Zhang, Yuzhu Zhao, Liqing TI Characterization of milk proteins-lutein complexes and the impact on lutein chemical stability SO FOOD CHEMISTRY LA English DT Article DE Whey protein isolate; Sodium caseinate; Lutein; Secondary structure; Chemical stability ID IN-WATER EMULSIONS; WHEY-PROTEIN; BETA-LACTOGLOBULIN; LIPID OXIDATION; CASEIN; RESVERATROL; ZEAXANTHIN; BINDING; ISOLATE; CAROTENOIDS AB In this study, the interaction of WPI (whey protein isolate) and SC (sodium caseinate) with hydrophobic lutein was investigated through UV-vis spectroscopy and circular dichroism (CD) as well as fluorescence. The effects on lutein's chemical stability were also examined. The decrease of turbidity of lutein suggested that lutein's aqueous solubility was improved after binding with milk proteins. CD analysis indicated lutein had little impact on the secondary structures of both proteins. Different preparation methods have significant impacts on the binding constant. Fluorescence results indicated that WPI and SC interact with lutein by hydrophobic contacts. Milk proteins have protective effects on lutein against oxidation and decomposition, and SC showed better capability in protecting lutein from oxidation than WPI during 16 days storage. The lutein's chemical stability was increased with increasing of proteins concentration. The results indicated that milk proteins may act as effective carriers for lipophilic nutraceuticals. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Yi, Jiang; Zhao, Liqing] Shenzhen Univ, Coll Chem & Environm Engn, Shenzhen 518060, Peoples R China. [Fan, Yuting] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China. [Yokoyama, Wallace; Zhang, Yuzhu] USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. RP Zhao, LQ (reprint author), Shenzhen Univ, Coll Chem & Environm Engn, Shenzhen 518060, Peoples R China. EM snowyzlj@163.com FU Shenzhen Dedicated Funding of Strategic Emerging Industry Development Program [JCYJ20140418091413576, CXZZ20150430093131635] FX This work was supported by the Shenzhen Dedicated Funding of Strategic Emerging Industry Development Program (JCYJ20140418091413576, CXZZ20150430093131635). NR 35 TC 5 Z9 5 U1 18 U2 116 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD JUN 1 PY 2016 VL 200 BP 91 EP 97 DI 10.1016/j.foodchem.2016.01.035 PG 7 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA DC2VV UT WOS:000369077300013 PM 26830565 ER PT J AU Shepherd, LVT Hackett, CA Alexander, CJ McNicol, JW Sungurtas, JA Mcrae, D McCue, KF Belknap, WR Davies, HV AF Shepherd, Louise Vida Traill Hackett, Christine Anne Alexander, Colin James McNicol, James William Sungurtas, Julia Anne Mcrae, Diane McCue, Kent Frank Belknap, William Richardson Davies, Howard Vivian TI Impact of light-exposure on the metabolite balance of transgenic potato tubers with modified glycoalkaloid biosynthesis SO FOOD CHEMISTRY LA English DT Article DE Genetic modification; Glycoalkaloids; Light; Metabolomics; Potato (Solanum tuberosum); UDP-galactose:solanidine galactosyltransferase ID CHLOROGENIC ACID CONTENT; SOLANUM-TUBEROSUM; SOLANIDINE GLUCOSYLTRANSFERASE; HUMAN-NUTRITION; FRESH; TRANSFORMATION; PLANT; GALACTOSYLTRANSFERASE; CHLOROPHYLL; EXPRESSION AB Metabolite profiling (liquid chromatography-mass spectrometry (LC-MS) and gas chromatography (GC-MS)) was used to assess the impact of light on the composition of transgenic potato (Solanum tuberosum L. cv. Desiree) with reduced glycoalkaloid content via the down-regulation of the SGT1 gene. Transgenic tubers exhibited an almost complete knock-out of alpha-solanine production and light had little impact on its accumulation. Levels of alpha-chaconine increased significantly in the peel of both the control and transgenic lines when exposed to light, particularly in the transgenic line. Major differences in metabolite profiles existed between outer and inner tuber tissues, and between light and dark-treated tubers. Many of the light-induced changes are explicable in terms of pathways known to be affected by stress responses. The impact of transgenesis on profiles was much less than that of tissue type or light and most differences were explicable in terms of the modification to the glycoalkaloid pathway. (C) 2016 Published by Elsevier Ltd. C1 [Shepherd, Louise Vida Traill; Sungurtas, Julia Anne; Mcrae, Diane; Davies, Howard Vivian] James Hutton Inst, Environm & Biochem Sci Grp, Dundee DD2 5DA, Scotland. [Hackett, Christine Anne; Alexander, Colin James; McNicol, James William] Biomath & Stat Scotland, Dundee DD2 5DA, Scotland. [McCue, Kent Frank; Belknap, William Richardson] USDA ARS, Crop Improvement & Genet, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. RP Shepherd, LVT (reprint author), James Hutton Inst, Environm & Biochem Sci Grp, Dundee DD2 5DA, Scotland. EM louise.shepherd@hutton.ac.uk RI Alexander, Colin/C-5802-2009 OI Alexander, Colin/0000-0003-4995-1678 FU SAFEFOODS [Food-CT-2004-506446]; Scottish Government's Rural and Environment Science and Analytical Services (RESAS) Division FX The authors would like to thank Paul Neave for technical support; Sean Connor, Gary Dobson, Tom Shepherd and Susan Verrall for advice with data processing and metabolite identification, and finally William Allwood for useful LC-MS/MSI advice and general manuscript discussion. This work was supported by SAFEFOODS (contract no. Food-CT-2004-506446), and by the Scottish Government's Rural and Environment Science and Analytical Services (RESAS) Division. NR 37 TC 2 Z9 2 U1 9 U2 85 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD JUN 1 PY 2016 VL 200 BP 263 EP 273 DI 10.1016/j.foodchem.2015.12.095 PG 11 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA DC2VV UT WOS:000369077300036 PM 26830588 ER PT J AU Kushwaha, V Saxena, K Verma, R Verma, SK Katoch, D Kumar, N Lal, B Murthy, PK Singh, B AF Kushwaha, Vikas Saxena, Kirti Verma, Richa Verma, Shiv K. Katoch, Deepali Kumar, Neeraj Lal, Brij Murthy, P. Kalpana Singh, Bikram TI Antifilarial activity of diterpenoids from Taxodium distichum SO PARASITES & VECTORS LA English DT Article DE Taxodium distichum; Brugia malayi; Labdane diterpenoids; In vitro assays; Macrofilaricide; Embryostatic; Mastomys coucha; Meriones unguiculatus; Diethylcarbamazine; Ivermectin ID LYMPHATIC FILARIAL PARASITE; BRUGIA-MALAYI; MASTOMYS-NATALENSIS; IN-VITRO; MERIONES-UNGUICULATUS; INFECTION; AGENTS; ACID; SPECTROSCOPY; SYSTEMS AB Background: Lymphatic filariasis caused by Wuchereria bancrofti, Brugia malayi and B. timori, is a debilitating disease with an adverse social and economic impact. The infection remains unabated in spite of treatment with existing antifilarial drugs diethylcarbamazine (DEC) and ivermectin which are chiefly microfilaricides. There is therefore, need for macrofilaricides, embryostatic agents and better microfilaricides. In the present study we explored the antifilarial potential of crude extract and its molecular fractions of the plant Taxodium distichum using in vitro assay systems and rodent models of B. malayi infection. Methods: Ethanolic extract (A001) of aerial parts of T. distichum was solvent fractionated and sub-fractionated. Four molecules, 3-Acetoxylabda-8(20), 13-diene-15-oic acid (K001), Beta-sitosterol (K002), labda-8(20), 13-diene-15-oic acid (K003) and Metasequoic acid A (K004) were isolated from the fractions and their structure determined by spectroscopic analysis. The extract, subfractions and molecules were evaluated for antifilarial activity against B. malayi by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) reduction and motility assays in vitro and in two animal models, Meriones unguiculatus and Mastomys coucha, harbouring B. malayi infection. Results: A001 was effective in killing microfilariae (mf) and adult worms in vitro. The diterpenoid K003 produced 100 % reduction in motility of both mf and adult worms and > 80 % inhibition in MTT reduction potential of adult female worms. In B. malayi-M. unguiculatus model, A001 killed all the adult worms in > 80 % of infected animals. K003 was embryostatic (> 95 %) in this model. In the B. malayi-M. coucha model, K003 killed similar to 54 % of adult worms (macrofilaricidal activity) and rendered > 36 % female worms sterile; it also stopped any further rise in microfilaraemia after day 42 post-initiation of treatment. Conclusion: Ethanolic extract of aerial parts of the plant T. distichum possesses potent antifilarial activity and the active principle was localised to K003 which showed significant macrofilaricidal activity and late suppression of peripheral microfilaraemia and some embryostatic activity. These findings indicate that labdane diterpenoid molecule(s) may provide valuable leads for design and development of new macrofilaricidal agent(s). To the best of our knowledge, this is the first report on antifilarial efficacy of products from the plant T. distichum. C1 [Kushwaha, Vikas; Saxena, Kirti; Verma, Richa; Verma, Shiv K.; Murthy, P. Kalpana] Cent Drug Res Inst, Div Parasitol, CSIR, New Campus,BS 10-1,Sect 10, Lucknow 226031, Uttar Pradesh, India. [Katoch, Deepali; Kumar, Neeraj; Singh, Bikram] CSIR Inst Himalayan Bioresource Technol, Nat Prod Chem & Proc Dev Div, Palampur 176061, Himachal Prades, India. [Lal, Brij] CSIR Inst Himalayan Bioresource Technol, Biodivers Div, Palampur 176061, Himachal Prades, India. [Verma, Shiv K.] ARS, USDA, APDL, BARC East Bldg 1001,10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Murthy, PK (reprint author), Cent Drug Res Inst, Div Parasitol, CSIR, New Campus,BS 10-1,Sect 10, Lucknow 226031, Uttar Pradesh, India.; Singh, B (reprint author), CSIR Inst Himalayan Bioresource Technol, Nat Prod Chem & Proc Dev Div, Palampur 176061, Himachal Prades, India. EM drpkmurthy@yahoo.com; bikramsingh@ihbt.res.in FU Network projects SPLENDID; Council of Scientific and Industrial Research, New Delhi, India [NWP 0037]; Indian Council of Medical Research, New Delhi; Department of Science and Technology, New Delhi, India FX The authors thank the Directors of CSIR-CDRI, Lucknow, U.P. and CSIR-IHBT, Palampur, H.P. India, for their keen interest and encouragement during the present investigation. The authors thank Dr. P.S.R. Murthy for critical review of the manuscript. This study was supported in part by the Network projects SPLENDID and NWP 0037 of Council of Scientific and Industrial Research, New Delhi, India. Statistical analysis was carried out by Dr. Mukesh Srivastava, Clinical and Experimental Medicine, Biometry section, CSIR-CDRI, Lucknow. VK and KS are thankful to Indian Council of Medical Research, New Delhi, for the award of Senior Research Fellowship. DK is thankful to Department of Science and Technology, New Delhi, India, for the award of Women Scientist Fellow. This manuscript bears communication numbers CSIR-CDRI: 9233 and CSIR-IHBT: 3551. NR 45 TC 0 Z9 0 U1 0 U2 2 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD MAY 31 PY 2016 VL 9 AR 312 DI 10.1186/s13071-016-1592-4 PG 11 WC Parasitology SC Parasitology GA DN8NZ UT WOS:000377337200001 PM 27245322 ER PT J AU Novak, K de Luis, M Saz, MA Longares, LA Serrano-Notivoli, R Raventos, J Cufar, K Gricar, J Di Filippo, A Piovesan, G Rathgeber, CBK Papadopoulos, A Smith, KT AF Novak, Klemen de Luis, Martin Saz, Miguel A. Longares, Luis A. Serrano-Notivoli, Roberto Raventos, Josep Cufar, Katarina Gricar, Jozica Di Filippo, Alfredo Piovesan, Gianluca Rathgeber, Cyrille B. K. Papadopoulos, Andreas Smith, Kevin T. TI Missing Rings in Pinus halepensis The Missing Link to Relate the Tree-Ring Record to Extreme Climatic Events SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE Aleppo pine; tree rings; climate-growth relationship; climate; extreme growth event; Mediterranean ID INTRAANNUAL DENSITY-FLUCTUATIONS; CAMBIAL ACTIVITY; FAGUS-SYLVATICA; WOOD FORMATION; GROWTH RINGS; SILVER FIR; PATTERNS; DROUGHT; SIGNALS; DRY AB Climate predictions for the Mediterranean Basin include increased temperatures, decreased precipitation, and increased frequency of extreme climatic events (ECE). These conditions are associated with decreased tree growth and increased vulnerability to pests and diseases. The anatomy of tree rings responds to these environmental conditions. Quantitatively, the width of a tree ring is largely determined by the rate and duration of cell division by the vascular cambium. In the Mediterranean climate, this division may occur throughout almost the entire year. Alternatively, cell division may cease during relatively cool and dry winters, only to resume in the same calendar year with milder temperatures and increased availability of water. Under particularly adverse conditions, no xylem may be produced in parts of the stem, resulting in a missing ring (MR). A dendrochronological network of Pinus halepensis was used to determine the relationship of MR to ECE. The network consisted of 113 sites, 1,509 trees, 2,593 cores, and 225,428 tree rings throughout the distribution range of the species. A total of 4,150 MR were identified. Binomial logistic regression analysis determined that MR frequency increased with increased cambial age. Spatial analysis indicated that the geographic areas of south-eastern Spain and northern Algeria contained the greatest frequency of MR. Dendroclimatic regression analysis indicated a non-linear relationship of MR to total monthly precipitation and mean temperature. MR are strongly associated with the combination of monthly mean temperature from previous October till current February and total precipitation from previous September till current May. They are likely to occur with total precipitation lower than 50 mm and temperatures higher than 5 degrees C. This conclusion is global and can be applied to every site across the distribution area. Rather than simply being a complication for dendrochronology, MR formation is a fundamental response of trees to adverse environmental conditions. The demonstrated relationship of MR formation to ECE across this dendrochronological network in the Mediterranean basin shows the potential of MR analysis to reconstruct the history of past climatic extremes and to predict future forest dynamics in a changing climate. C1 [Novak, Klemen; de Luis, Martin; Saz, Miguel A.; Longares, Luis A.; Serrano-Notivoli, Roberto] Univ Zaragoza, Inst Invest Ciencias Ambientales, Dept Geog & Reg Planning, Zaragoza, Spain. [Novak, Klemen; Raventos, Josep] Univ Alicante, Dept Ecol, E-03080 Alicante, Spain. [Cufar, Katarina] Univ Ljubljana, Biotech Fac, Dept Wood Sci & Technol, Ljubljana, Slovenia. [Gricar, Jozica] Slovenian Forestry Inst, Ljubljana, Slovenia. [Di Filippo, Alfredo; Piovesan, Gianluca] Univ Tuscia, Dept Agr & Forestry Sci DAFNE, Dendrol Lab, Viterbo, Italy. [Rathgeber, Cyrille B. K.] INRA, AgroParisTech, LERFoB, Nancy, France. [Papadopoulos, Andreas] TEI Stereas Elladas, Dept Forestry & Nat Environm Management, Karpenissi, Greece. [Smith, Kevin T.] Forest Serv, USDA, No Res Stn, Durham, NH USA. RP Novak, K (reprint author), Univ Zaragoza, Inst Invest Ciencias Ambientales, Dept Geog & Reg Planning, Zaragoza, Spain.; Novak, K (reprint author), Univ Alicante, Dept Ecol, E-03080 Alicante, Spain. EM knovak@unizar.es RI Piovesan, Gianluca/C-2720-2008; Di Filippo, Alfredo/G-1794-2010; Rathgeber, Cyrille/A-6510-2009 OI Piovesan, Gianluca/0000-0002-3214-0839; Di Filippo, Alfredo/0000-0001-5863-8339; Rathgeber, Cyrille/0000-0001-7359-8320 FU Spanish Ministry of Education and Science - FEDER program [CGL2012-31668, CGL2015-69985-R]; Slovenian Research Agency [P4-0015, P4-0107]; University of Ljubljana; University of Alicante; USDA Forest Service; COST (European Cooperation in Science and Technology) FX This study was supported by Spanish Ministry of Education and Science co-funded by FEDER program (projects: CGL2012-31668 and CGL2015-69985-R), by the Slovenian Research Agency (programs P4-0015 and P4-0107), by the LLP ERASMUS bilateral agreement between the University of Ljubljana and the University of Alicante, and the USDA Forest Service. The article is based upon work from COST Action FP1106 STReESS, supported by COST (European Cooperation in Science and Technology). NR 61 TC 4 Z9 4 U1 7 U2 14 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD MAY 31 PY 2016 VL 7 AR 727 DI 10.3389/fpls.2016.00727 PG 11 WC Plant Sciences SC Plant Sciences GA DN1VF UT WOS:000376852800001 PM 27303421 ER PT J AU Rodgers-Melnick, E Vera, DL Bass, HW Buckler, ES AF Rodgers-Melnick, Eli Vera, Daniel L. Bass, Hank W. Buckler, Edward S. TI Open chromatin reveals the functional maize genome SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE chromatin; biased gene conversion; maize; recombination; variance partitioning ID BIASED GENE CONVERSION; SURPRISING FITNESS CONSEQUENCES; MEIOTIC RECOMBINATION; DNA ELEMENTS; TRANSPOSON INSERTION; REGULATORY DNA; PHASE-CHANGE; IDENTIFICATION; ARABIDOPSIS; METHYLATION AB Cellular processes mediated through nuclear DNA must contend with chromatin. Chromatin structural assays can efficiently integrate information across diverse regulatory elements, revealing the functional noncoding genome. In this study, we use a differential nuclease sensitivity assay based on micrococcal nuclease (MNase) digestion to discover open chromatin regions in the maize genome. We find that maize MNase-hypersensitive (MNase HS) regions localize around active genes and within recombination hotspots, focusing biased gene conversion at their flanks. Although MNase HS regions map to less than 1% of the genome, they consistently explain a remarkably large amount (similar to 40%) of heritable phenotypic variance in diverse complex traits. MNase HS regions are therefore on par with coding sequences as annotations that demarcate the functional parts of the maize genome. These results imply that less than 3% of the maize genome (coding and MNase HS regions) may give rise to the overwhelming majority of phenotypic variation, greatly narrowing the scope of the functional genome. C1 [Rodgers-Melnick, Eli; Buckler, Edward S.] Cornell Univ, Inst Genom Divers, Ithaca, NY 14853 USA. [Vera, Daniel L.; Bass, Hank W.] Florida State Univ, Dept Biol Sci, B-157, Tallahassee, FL 32306 USA. [Vera, Daniel L.] Florida State Univ, Ctr Genom & Personalized Med, Tallahassee, FL 32306 USA. [Buckler, Edward S.] ARS, USDA, Ithaca, NY 14853 USA. RP Rodgers-Melnick, E; Buckler, ES (reprint author), Cornell Univ, Inst Genom Divers, Ithaca, NY 14853 USA.; Buckler, ES (reprint author), ARS, USDA, Ithaca, NY 14853 USA. EM eli.rodgers-melnick@pioneer.com; esb33@cornell.edu OI Buckler, Edward/0000-0002-3100-371X; Bass, Hank/0000-0003-0522-0881 NR 42 TC 5 Z9 5 U1 10 U2 20 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAY 31 PY 2016 VL 113 IS 22 BP E3177 EP E3184 DI 10.1073/pnas.1525244113 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DN0VL UT WOS:000376784600018 PM 27185945 ER PT J AU Penny, C Grothendick, B Zhang, L Borror, CM Barbano, D Cornelius, AJ Gilpin, BJ Fagerquist, CK Zaragoza, WJ Jay-Russell, MT Lastovica, AJ Ragimbeau, C Cauchie, HM Sandrin, TR AF Penny, Christian Grothendick, Beau Zhang, Lin Borror, Connie M. Barbano, Duane Cornelius, Angela J. Gilpin, Brent J. Fagerquist, Clifton K. Zaragoza, William J. Jay-Russell, Michele T. Lastovica, Albert J. Ragimbeau, Catherine Cauchie, Henry-Michel Sandrin, Todd R. TI A Designed Experiments Approach to Optimizing MALDI-TOF MS Spectrum Processing Parameters Enhances Detection of Antibiotic Resistance in Campylobacter jejuni SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE MALDI-TOF MS; Campylobacter jejuni; antimicrobial resistance; antibiotic resistance; designed experiments; spectrum processing ID FLIGHT MASS-SPECTROMETRY; DESORPTION IONIZATION-TIME; ANTIMICROBIAL RESISTANCE; PROTEOMIC IDENTIFICATION; CLINICAL MICROBIOLOGY; RAPID DETECTION; BACTERIA; CLASSIFICATION; MECHANISMS; REPRODUCIBILITY AB MALDI-TOF MS has been utilized as a reliable and rapid tool for microbial fingerprinting at the genus and species levels. Recently, there has been keen interest in using MALDITOF MS beyond the genus and species levels to rapidly identify antibiotic resistant strains of bacteria. The purpose of this study was to enhance strain level resolution for Campylobacter jejuni through the optimization of spectrum processing parameters using a series of designed experiments. A collection of 172 strains of C. jejuni were collected from Luxembourg, New Zealand, North America, and South Africa, consisting of four groups of antibiotic resistant isolates. The groups included: (1) 65 strains resistant to cefoperazone (2) 26 resistant to cefoperazone and beta-lactams (3) 5 strains resistant to cefoperazone, beta-lactams, and tetracycline, and (4) 76 strains resistant to cefoperazone, teicoplanin, amphotericin, B and cephalothin. Initially, a model set of 16 strains (three biological replicates and three technical replicates per isolate, yielding a total of 144 spectra) of C. jejuni was subjected to each designed experiment to enhance detection of antibiotic resistance. The most optimal parameters were applied to the larger collection of 172 isolates (two biological replicates and three technical replicates per isolate, yielding a total of 1,031 spectra). We observed an increase in antibiotic resistance detection whenever either a curve based similarity coefficient (Pearson or ranked Pearson) was applied rather than a peak based (Dice) and/or the optimized preprocessing parameters were applied. Increases in antimicrobial resistance detection were scored using the jackknife maximum similarity technique following cluster analysis. From the first four groups of antibiotic resistant isolates, the optimized preprocessing parameters increased detection respective to the aforementioned groups by: (1) 5% (2) 9% (3) 10%, and (4) 2%. An additional second categorization was created from the -collection-consisting-of-31-strains-resistant-to-beta-lactams-and-141-strains-sensitive to-beta-lactams.-Applying-optimal-preprocessing-parameters,-beta-lactam-resistance detection-was-increased-by-34%.-These-results-suggest-that-spectrum-processing parameters,-which-are-rarely-optimized-or-adjusted,-affect-the-performance-of-MALDITOF MS--based-detection-of-antibiotic-resistance-and-can-be-fine-tuned-to-enhance screening-performance. C1 [Penny, Christian; Cauchie, Henry-Michel] Luxembourg Inst Sci & Technol, Environm Res & Innovat Dept, Esch Sur Alzette, Luxembourg. [Grothendick, Beau; Zhang, Lin; Borror, Connie M.; Barbano, Duane; Sandrin, Todd R.] Arizona State Univ, Sch Math & Nat Sci, Phoenix, AZ USA. [Cornelius, Angela J.; Gilpin, Brent J.] Inst Environm Sci & Res, Christchurch, New Zealand. [Fagerquist, Clifton K.; Zaragoza, William J.] ARS, USDA, Albany, CA USA. [Jay-Russell, Michele T.] Univ Calif Davis, Western Ctr Food Safety, Davis, CA 95616 USA. [Lastovica, Albert J.] Univ Western Cape, Dept Biotechnol, ZA-7535 Bellville, South Africa. [Ragimbeau, Catherine] Lab Natl Sante, Epidemiol Surveillance Infect Dis, Dudelange, Luxembourg. RP Penny, C (reprint author), Luxembourg Inst Sci & Technol, Environm Res & Innovat Dept, Esch Sur Alzette, Luxembourg.; Sandrin, TR (reprint author), Arizona State Univ, Sch Math & Nat Sci, Phoenix, AZ USA. EM christian.penny@list.lu; todd.sandrin@asu.edu RI Cauchie, Henry-Michel/K-8123-2014 OI Cauchie, Henry-Michel/0000-0003-4780-6100 FU National Research Fund of Luxembourg (FNR) [INTER/MOBILITY/14/7356524]; Arizona State University's New College undergraduate research program, NCUIRE (New College Undergraduate Inquiry and Research Experiences); Salt River Project (SRP, USA) FX CP received specific funding from the National Research Fund of Luxembourg (FNR; grant reference INTER/MOBILITY/14/7356524). This study was supported by the Arizona State University's New College undergraduate research program, NCUIRE (New College Undergraduate Inquiry and Research Experiences) and the Salt River Project (SRP, USA). NR 47 TC 3 Z9 3 U1 3 U2 9 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD MAY 31 PY 2016 VL 7 AR 818 DI 10.3389/fmicb.2016.00818 PG 9 WC Microbiology SC Microbiology GA DN0YC UT WOS:000376792000002 PM 27303397 ER PT J AU Mullen, ER Rutschman, P Pegram, N Patt, JM Adamczyk, JJ Johanson, R AF Mullen, Emma R. Rutschman, Phillip Pegram, Nathan Patt, Joseph M. Adamczyk, John J., Jr. Johanson, Ric TI Laser system for identification, tracking, and control of flying insects SO OPTICS EXPRESS LA English DT Article ID CANDIDATUS LIBERIBACTER ASIATICUS; CITRUS; MOSQUITOS; FLORIDA AB Flying insects are common vectors for transmission of pathogens and inflict significant harm to humans and agricultural production in many parts of the world. We present proof of principle for an optical system capable of highly specific vector control. This system utilizes a combination of optical sources, detectors, and sophisticated software to search, detect, and identify flying insects in real-time, with the capability of eradication using a lethal laser pulse. We present data on two insect species to show species distinction; Diaphorina citri, a vector of the causal agent of citrus greening disease, and Anopheles stephensi, a malaria vector. (C)2016 Optical Society of America C1 [Mullen, Emma R.; Rutschman, Phillip; Pegram, Nathan; Johanson, Ric] Intellectual Ventures Lab, 14360 SE Eastgate Way, Bellevue, WA 98007 USA. [Patt, Joseph M.] ARS, USDA, US Hort Lab, Ft Pierce, FL USA. [Adamczyk, John J., Jr.] ARS, USDA, Thad Cochran Southern Hort Lab, Poplarville, MS USA. RP Johanson, R (reprint author), Intellectual Ventures Lab, 14360 SE Eastgate Way, Bellevue, WA 98007 USA. EM ejohanson@intven.com FU Bill and Melinda Gates through the Global Good Fund; USDA Agricultural Research Service FX The authors thank Bill and Melinda Gates for their support and sponsorship through the Global Good Fund. Additional funding for this study was provided by the USDA Agricultural Research Service. We are grateful to A. Gomezplata, O. Zamora, D. Stockton, and G. Betts for their assistance in this study. NR 30 TC 2 Z9 2 U1 3 U2 9 PU OPTICAL SOC AMER PI WASHINGTON PA 2010 MASSACHUSETTS AVE NW, WASHINGTON, DC 20036 USA SN 1094-4087 J9 OPT EXPRESS JI Opt. Express PD MAY 30 PY 2016 VL 24 IS 11 BP 1828 EP 1838 DI 10.1364/OE.24.011828 PG 11 WC Optics SC Optics GA DO0KM UT WOS:000377467800048 PM 27410106 ER PT J AU Wagle, P Gowda, PH Xiao, XM Anup, KC AF Wagle, Pradeep Gowda, Prasanna H. Xiao, Xiangming Anup, K. C. TI Parameterizing ecosystem light use efficiency and water use efficiency to estimate maize gross primary production and evapotranspiration using MODIS EVI SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Artificial neural network; Eddy covariance; Empirical and statistical models; Remote sensing; Seasonal and interannual variability; Vegetation indices ID SURFACE-ENERGY BALANCE; PHOTOCHEMICAL REFLECTANCE INDEX; SOYBEAN CROPPING SYSTEMS; VEGETATION INDEXES; CARBON-DIOXIDE; BOREAL FORESTS; MODEL; SATELLITE; FLUX; VAPOR AB Quantifying global carbon and water balances requires accurate estimation of gross primary production (GPP) and evapotranspiration (ET), respectively, across space and time. Models that are based on the theory of light use efficiency (LUE) and water use efficiency (WUE) have emerged as efficient methods for predicting GPP and ET, respectively. Currently, LUE and WUE estimates are obtained from biome-specific look-up tables and coarse resolution remote sensing data with large uncertainties. The major objective of this study was to parameterize eddy covariance tower-based ecosystem LUE (ELUEEc), defined as the ratio of tower-based GPP (GPPEc) to photosynthetically active radiation (PAR), and ecosystem WUE (EWUEEc), defined as the ratio of GPPEc to tower-based ET (ETEc), using the Moderate Resolution Imaging Spectroradiometer(MODIS)-derived enhanced vegetation index (EVI) for predicting maize (Zea mays L) GPP and ET, respectively. Three adjacent AmeriFlux maize sites with different rotations (continuous maize vs. annual rotation of maize and soybean, Glycine max L.) and water management practices (rainfed vs. irrigated) located near Mead, NE, USA were selected. The EVI tracked the seasonal variations of ELUEEC (R-2=0.83) and EWUEEC (R-2 =0.74) across sites, indicating that EVI can be explicitly used as a measure of ELUEEC and EWUEEC. The predicted GPP (GPFEwE) using the parameterized ELUE model correlated well with GPP(EC) (slope = 1.0, R-2 =0.83, and RMSE = 2.85 gC m(-2) d(-1)) and was significantly improved when compared to widely used models that estimate GPP by integrating EVI and climate variables (Greenness and Radiation, Temperature and Greenness, and Vegetation Index) and the standard MOD17 GPP product. Similarly, the predicted ET (ETEwuE) using the parameterized EWUE correlated well with ETEc (slope =1.02, R-2=0.62, and RMSE = 0.83 mm ET-1) and was significantly improved when compared to the standard MOD16 ET product. Preliminary data demonstrate that ELUE and EWUE can be parameterized using EVI, offering new methods for predicting GPP and ET. (C) 2016 Elsevier B.V. All rights reserved. C1 [Wagle, Pradeep; Xiao, Xiangming] Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK 73019 USA. [Wagle, Pradeep; Xiao, Xiangming] Univ Oklahoma, Ctr Spatial Anal, Norman, OK 73019 USA. [Wagle, Pradeep; Gowda, Prasanna H.] USDA ARS, Grazinglands Res Lab, El Reno, OK 73036 USA. [Xiao, Xiangming] Fudan Univ, Inst Biodivers Sci, Shanghai 200433, Peoples R China. [Anup, K. C.] Oklahoma State Univ, Dept Nat Resource Ecol & Management, Stillwater, OK 74078 USA. RP Wagle, P (reprint author), Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK 73019 USA.; Wagle, P (reprint author), Univ Oklahoma, Ctr Spatial Anal, Norman, OK 73019 USA.; Wagle, P (reprint author), USDA ARS, Grazinglands Res Lab, El Reno, OK 73036 USA. EM pradeep.wagle@ou.edu FU USDA National Institute for Food and Agriculture (NIFA)'s Agriculture and Food Research Initiative (AFRI), Regional Approaches for Adaptation to and Mitigation of Climate Variability and Change [2013-69002]; National Science Foundation EPSCoR [IIA-1301789] FX This study was supported in part by a research grant (Project No. 2013-69002) through the USDA National Institute for Food and Agriculture (NIFA)'s Agriculture and Food Research Initiative (AFRI), Regional Approaches for Adaptation to and Mitigation of Climate Variability and Change, and a research grant (IIA-1301789) from the National Science Foundation EPSCoR. Flux and climate data were obtained from the AmeriFlux database (http://ameriflux.lbl.gov/). We would like to thank Dr. Andrew Suyker (University of Nebraska) for the Mead flux tower data. NR 65 TC 1 Z9 1 U1 9 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD MAY 28 PY 2016 VL 222 BP 87 EP 97 DI 10.1016/j.agrformet.2016.03.009 PG 11 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA DK8FA UT WOS:000375161400008 ER PT J AU Li, R Zhang, YF Polk, DB Tomasula, PM Yan, F Liu, LS AF Li, Ran Zhang, Yufeng Polk, D. Brent Tomasula, Peggy M. Yan, Fang Liu, LinShu TI Preserving viability of Lactobacillus rhamnosus GG in vitro and in vivo by a new encapsulation system SO JOURNAL OF CONTROLLED RELEASE LA English DT Article DE Colitis; Encapsulation; Glucose; Lactobacillus rhamnosus GG; Pectin; Viability ID PLACEBO-CONTROLLED TRIAL; ACTIVE ULCERATIVE-COLITIS; PROBIOTIC BACTERIA; POUCHITIS ONSET; DRUG-DELIVERY; DOUBLE-BLIND; SURVIVAL; MICROENCAPSULATION; STABILITY; THERAPY AB Probiotics have shown beneficial effects on health and prevention of diseases in humans. However, a concern for application of probiotics is the loss of viability during storage and gastrointestinal transit. The aim of this study was to develop an encapsulation system to preserve viability of probiotics when they are administrated orally and apply Lactobacillus rhamnosus GG (LGG) as a probiotic model to evaluate the effectiveness of this approach using in vitro and in vivo experiments. LGG was encapsulated in hydrogel beads prepared using pectin, a food grade polysaccharide, glucose, and calcium chloride, and lyophilized by freeze-drying. Encapsulated LGG was cultured in vitro under the condition that mimicked the physiological environment of the human gastrointestinal tract. Compared to non-encapsulated LGG, encapsulation increased tolerance of LGG in the acid condition, protected LGG from protease digestion, and improved shelf time when stored at the ambient condition, in regard of survivability and production of p40, a known LGG-derived protein involved in LGG's beneficial effects on intestinal homeostasis. To evaluate the effects of encapsulation on p40 production in vivo and prevention of intestinal inflammation by LGG, mice were gavaged with LGG containing beads and treated with dextran sulphate sodium (DSS) to induce intestinal injury and colitis. Compared to non-encapsulated LGG, encapsulated LGG enhanced more p40 production in mice, and exerted higher levels of effects on prevention of DSS-induced colonic injury and colitis and suppression of pro-inflammatory cytokine production. These data indicated that the encapsulation system developed in this study preserves viability of LGG in vitro and in vivo, leading to longer shelf time and enhancing the functions of LGG in the gastrointestinal tract. Thus, this encapsulation approach may have the potential application for improving efficacy of probiotics. (C) 2016 Elsevier B.V. All rights reserved. C1 [Li, Ran; Zhang, Yufeng] Tianjin Polytech Univ, State Key Lab Hollow Fiber Membrane Mat & Proc, 399 Binshuixi Rd, Tianjin 300387, Peoples R China. [Li, Ran; Tomasula, Peggy M.; Liu, LinShu] USDA ARS, Eastern Reg Res Ctr, Dairy & Funct Foods Res Unit, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Polk, D. Brent] Univ So Calif, Dept Pediat, 4650 Sunset Blvd, Los Angeles, CA 90027 USA. [Polk, D. Brent] Univ So Calif, Dept Biochem & Mol Biol, 4650 Sunset Blvd, Los Angeles, CA 90027 USA. [Polk, D. Brent] Childrens Hosp Los Angeles, Saban Res Inst, 4650 Sunset Blvd, Los Angeles, CA 90027 USA. [Yan, Fang] Vanderbilt Univ, Med Ctr, Dept Pediat, Div Gastroenterol Hepatol & Nutr, 2215 Garland Ave, Nashville, TN 37232 USA. RP Liu, LS (reprint author), USDA ARS, Eastern Reg Res Ctr, Dairy & Funct Foods Res Unit, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA.; Yan, F (reprint author), Vanderbilt Univ, Med Ctr, Dept Pediat, Div Gastroenterol Hepatol & Nutr, 2215 Garland Ave, Nashville, TN 37232 USA. EM fang.yan@vanderbilt.edu; Linshu.liu@ars.usda.gov FU NIH [R01DK081134]; Vanderbilt University Medical Center's Digestive Disease Research Center - NIH [P30DK058404] FX The authors gratefully acknowledge the technical support of Dr. Lihan Huang, Mr. Joseph Uknalis, and Ms. Audrey Thomas-Gahring of ERRC-USDA. This work was supported by NIH grants R01DK081134 (F.Y.) and core services performed through Vanderbilt University Medical Center's Digestive Disease Research Center supported by NIH grant P30DK058404. NR 47 TC 3 Z9 3 U1 20 U2 46 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-3659 EI 1873-4995 J9 J CONTROL RELEASE JI J. Control. Release PD MAY 28 PY 2016 VL 230 BP 79 EP 87 DI 10.1016/j.jconrel.2016.04.009 PG 9 WC Chemistry, Multidisciplinary; Pharmacology & Pharmacy SC Chemistry; Pharmacology & Pharmacy GA DL1SN UT WOS:000375412500009 PM 27063422 ER PT J AU Saminathan, T Bodunrin, A Singh, NV Devarajan, R Nimmakayala, P Jeff, M Aradhya, M Reddy, UK AF Saminathan, Thangasamy Bodunrin, Abiodun Singh, Nripendra V. Devarajan, Ramajayam Nimmakayala, Padma Jeff, Moersfelder Aradhya, Mallikarjuna Reddy, Umesh K. TI Genome-wide identification of microRNAs in pomegranate (Punica granatum L.) by high-throughput sequencing SO BMC PLANT BIOLOGY LA English DT Article DE Pomegranate; MicroRNA; Stem-loop RT-qPCR; Fruit development; High-throughput sequencing ID TOMATO FRUIT-DEVELOPMENT; ANTHOCYANIN BIOSYNTHESIS; ARABIDOPSIS-THALIANA; FLAVONOID BIOSYNTHESIS; RESPONSIVE MICRORNAS; CONSERVED MICRORNAS; SUCROSE TRANSPORTER; PLANT DEVELOPMENT; GENE-EXPRESSION; COLORFUL MODEL AB Background: MicroRNAs (miRNAs), a class of small non-coding endogenous RNAs that regulate gene expression post-transcriptionally, play multiple key roles in plant growth and development and in biotic and abiotic stress response. Knowledge and roles of miRNAs in pomegranate fruit development have not been explored. Results: Pomegranate, which accumulates a large amount of anthocyanins in skin and arils, is valuable to human health, mainly because of its antioxidant properties. In this study, we developed a small RNA library from pooled RNA samples from young seedlings to mature fruits and identified both conserved and pomegranate-specific miRNA from 29,948,480 high-quality reads. For the pool of 15-to 30-nt small RNAs, similar to 50 % were 24 nt. The miR157 family was the most abundant, followed by miR156, miR166, and miR168, with variants within each family. The base bias at the first position from the 5' end had a strong preference for U for most 18-to 26-nt sRNAs but a preference for A for 18-nt sRNAs. In addition, for all 24-nt sRNAs, the nucleotide U was preferred (97 %) in the first position. Stem-loop RT-qPCR was used to validate the expression of the predominant miRNAs and novel miRNAs in leaves, male and female flowers, and multiple fruit developmental stages; miR156, miR156a, miR159a, miR159b, and miR319b were upregulated during the later stages of fruit development. Higher expression of miR156 in later fruit developmental may positively regulate anthocyanin biosynthesis by reducing SPL transcription factor. Novel miRNAs showed variation in expression among different tissues. These novel miRNAs targeted different transcription factors and hormone related regulators. Gene ontology and KEGG pathway analyses revealed predominant metabolic processes and catalytic activities, important for fruit development. In addition, KEGG pathway analyses revealed the involvement of miRNAs in ascorbate and linolenic acid, starch and sucrose metabolism; RNA transport; plant hormone signaling pathways; and circadian clock. Conclusion: Our first and preliminary report of miRNAs will provide information on the synthesis of biochemical compounds of pomegranate for future research. The functions of the targets of the novel miRNAs need further investigation. C1 [Saminathan, Thangasamy; Bodunrin, Abiodun; Nimmakayala, Padma; Reddy, Umesh K.] West Virginia State Univ, Gus R Douglass Inst, Dept Biol, Institute, WV 25112 USA. [Singh, Nripendra V.] ICAR Res Complex, Natl Res Ctr Pomegranate, Solapur 413255, Maharashtra, India. [Devarajan, Ramajayam] ICAR Res Complex, Indian Inst Oil Palm Res, West Godavari 534450, Andhra Pradesh, India. [Jeff, Moersfelder; Aradhya, Mallikarjuna] Univ Calif Davis, USDA ARS, Natl Clonal Germplasm Repository, Davis, CA 95616 USA. RP Reddy, UK (reprint author), West Virginia State Univ, Gus R Douglass Inst, Dept Biol, Institute, WV 25112 USA. EM ureddy@wvstateu.edu FU USDA-NIFA grant [2013-04023]; Indian Council of Agricultural Research (ICAR); National Agricultural Innovation Project, ICAR FX This project was supported by the USDA-NIFA grant (Proposal no. 2013-04023) and additionally supported by the Indian Council of Agricultural Research (ICAR). The authors thank the National Agricultural Innovation Project, ICAR, for sponsoring visits of Nripendra V. Singh and Ramajayam Devarajan. We are also grateful to Guru Jagadeeswaran for his suggestions to improve the quality of the manuscript significantly. NR 94 TC 2 Z9 2 U1 14 U2 30 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD MAY 26 PY 2016 VL 16 AR 122 DI 10.1186/s12870-016-0807-3 PG 16 WC Plant Sciences SC Plant Sciences GA DN7OT UT WOS:000377267300004 PM 27230657 ER PT J AU Dubey, JP Ferreira, LR Alsaad, M Verma, SK Alves, DA Holland, GN McConkey, GA AF Dubey, Jitender P. Ferreira, Leandra R. Alsaad, Mohammad Verma, Shiv K. Alves, Derron A. Holland, Gary N. McConkey, Glenn A. TI Experimental Toxoplasmosis in Rats Induced Orally with Eleven Strains of Toxoplasma gondii of Seven Genotypes: Tissue Tropism, Tissue Cyst Size, Neural Lesions, Tissue Cyst Rupture without Reactivation, and Ocular Lesions SO PLOS ONE LA English DT Article ID CONGENITAL TRANSMISSION; PARASITIC INFECTION; GENETIC DIVERSITY; SANTA ISABEL; MICE; OOCYSTS; CATS; BRAZIL; SCHIZOPHRENIA; PATHOGENICITY AB Background The protozoan parasite Toxoplasma gondii is one of the most widely distributed and successful parasites. Toxoplasma gondii alters rodent behavior such that infected rodents reverse their fear of cat odor, and indeed are attracted rather than repelled by feline urine. The location of the parasite encysted in the brain may influence this behavior. However, most studies are based on the highly susceptible rodent, the mouse. Methodology/Principal Findings Latent toxoplasmosis was induced in rats (10 rats per T. gondii strains) of the same age, strain, and sex, after oral inoculation with oocysts (natural route and natural stage of infection) of 11 T. gondii strains of seven genotypes. Rats were euthanized at two months post inoculation (p.i.) to investigate whether the parasite genotype affects the distribution, location, tissue cyst size, or lesions. Tissue cysts were enumerated in different regions of the brains, both in histological sections as well in saline homogenates. Tissue cysts were found in all regions of the brain. The tissue cyst density in different brain regions varied extensively between rats with many regions highly infected in some animals. Overall, the colliculus was most highly infected although there was a large amount of variability. The cerebral cortex, thalamus, and cerebellum had higher tissue cyst densities and two strains exhibited tropism for the colliculus and olfactory bulb. Histologically, lesions were confined to the brain and eyes. Tissue cyst rupture was frequent with no clear evidence for reactivation of tachyzoites. Ocular lesions were found in 23 (25%) of 92 rat eyes at two months p.i. The predominant lesion was focal inflammation in the retina. Tissue cysts were seen in the sclera of one and in the optic nerve of two rats. The choroid was not affected. Only tissue cysts, not active tachyzoite infections, were detected. Tissue cysts were seen in histological sections of tongue of 20 rats but not in myocardium and leg muscle. Conclusion/Significance This study reevaluated in depth the rat model of toxoplasmosis visualizing cyst rupture and clarified many aspects of the biology of the parasite useful for future investigations. C1 [Dubey, Jitender P.; Ferreira, Leandra R.; Verma, Shiv K.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD USA. [Alsaad, Mohammad; McConkey, Glenn A.] Univ Leeds, Fac Biol Sci, Miall Bldg,Clarendon Way, Leeds, W Yorkshire, England. [Alves, Derron A.] Vet Pathol Serv Joint Pathol Ctr, 606 Stephen Sitter Ave, Silver Spring, MD USA. [Holland, Gary N.] Univ Calif Los Angeles, Stein Eye Inst, Ocular Inflammatory Dis Ctr, Los Angeles, CA USA. [Holland, Gary N.] Univ Calif Los Angeles, David Geffen Sch Med, Dept Ophthalmol, Los Angeles, CA 90095 USA. RP Dubey, JP (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD USA. EM Jitender.Dubey@ars.usda.gov NR 76 TC 0 Z9 0 U1 10 U2 15 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 26 PY 2016 VL 11 IS 5 AR e0156255 DI 10.1371/journal.pone.0156255 PG 26 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DN2GQ UT WOS:000376882500095 PM 27228262 ER PT J AU Wu, FN Cen, YJ Wallis, CM Trumble, JT Prager, S Yokomi, R Zheng, Z Deng, XL Chen, JC Liang, GW AF Wu, Fengnian Cen, Yijing Wallis, Christopher M. Trumble, John T. Prager, Sean Yokomi, Ray Zheng, Zheng Deng, Xiaoling Chen, Jianchi Liang, Guangwen TI The Complete Mitochondrial Genome Sequence of Bactericera cockerelli and Comparison with Three Other Psylloidea Species SO PLOS ONE LA English DT Article ID TRANSFER-RNA GENES; HEMIPTERA TRIOZIDAE; UNITED-STATES; POTATO; PHYLOGENY; DNA; PROGRAM; SULC; PSYLLIDS; AMERICA AB Potato psyllid (Bactericera cockerelli) is an important pest of potato, tomato and pepper. Not only could a toxin secreted by nymphs results in serious phytotoxemia in some host plants, but also over the past few years B. cockerelli was shown to transmit "Candidatus Liberibacter solanacearum", the putative bacterial pathogen of potato zebra chip (ZC) disease, to potato and tomato. ZC has caused devastating losses to potato production in the western U.S., Mexico, and elsewhere. New knowledge of the genetic diversity of the B. cockerelli is needed to develop improved strategies to manage pest populations. Mitochondrial genome (mitogenome) sequencing provides important knowledge about insect evolution and diversity in and among populations. This report provides the first complete B. cockerelli mitogenome sequence as determined by next generation sequencing technology (Illumina MiSeq). The circular B. cockerelli mitogenome had a size of 15,220 bp with 13 protein-coding gene (PCGs), 2 ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and a non-coding region of 975 bp. The overall gene order of the B. cockerelli mitogenome is identical to three other published Psylloidea mitogenomes: one species from the Triozidae, Paratrioza sinica; and two species from the Psyllidae, Cacopsylla coccinea and Pachypsylla venusta. This suggests all of these species share a common ancestral mitogenome. However, sequence analyses revealed differences between and among the insect families, in particular a unique region that can be folded into three stem-loop secondary structures present only within the B. cockerelli mitogenome. A phylogenetic tree based on the 13 PCGs matched an existing taxonomy scheme that was based on morphological characteristics. The available complete mitogenome sequence makes it accessible to all genes for future population diversity evaluation of B. cockerelli. C1 [Wu, Fengnian; Cen, Yijing; Zheng, Zheng; Deng, Xiaoling; Liang, Guangwen] South China Agr Univ, Key Lab Microbial Signals & Dis Control, Coll Agr, Lab Insect Ecol Guangdong Prov, Guangzhou, Guangdong, Peoples R China. [Wu, Fengnian; Wallis, Christopher M.; Yokomi, Ray; Zheng, Zheng; Chen, Jianchi] ARS, San Joaquin Valley Agr Sci Ctr, USDA, Parlier, CA USA. [Trumble, John T.; Prager, Sean] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. RP Liang, GW (reprint author), South China Agr Univ, Key Lab Microbial Signals & Dis Control, Coll Agr, Lab Insect Ecol Guangdong Prov, Guangzhou, Guangdong, Peoples R China.; Chen, JC (reprint author), ARS, San Joaquin Valley Agr Sci Ctr, USDA, Parlier, CA USA. EM jianchi.chen@ars.usda.gov; gwliang@scau.edu.cn FU Chinese Modern Agricultural Technology Systems [CARS-27]; California Citrus Research Board FX This study was supported by the Chinese Modern Agricultural Technology Systems (CARS-27) (XD); California Citrus Research Board (JC). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 47 TC 0 Z9 0 U1 8 U2 13 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 26 PY 2016 VL 11 IS 5 AR e0155318 DI 10.1371/journal.pone.0155318 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DN2GQ UT WOS:000376882500015 PM 27227976 ER PT J AU Cass, CL Lavell, AA Santoro, N Foster, CE Karlen, SD Smith, RA Ralph, J Garvin, DF Sedbrook, JC AF Cass, Cynthia L. Lavell, Anastasiya A. Santoro, Nicholas Foster, Cliff E. Karlen, Steven D. Smith, Rebecca A. Ralph, John Garvin, David F. Sedbrook, John C. TI Cell Wall Composition and Biomass Recalcitrance Differences Within a Genotypically Diverse Set of Brachypodium distachyon Inbred Lines SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE bioenergy; grass; polysaccharide; hemicellulose; lignin; digestibility; vernalization; Pooideae ID BROMIDE SPECTROPHOTOMETRIC METHOD; ARABIDOPSIS-THALIANA; LIGNIN CONCENTRATION; NATURAL VARIATION; FLOWERING TIME; MODEL SYSTEM; GRASS; SACCHARIFICATION; CONVERSION; QUALITY AB Brachypodium distachyon (Brachypodium) has emerged as a useful model system for studying traits unique to graminaceous species including bioenergy crop grasses owing to its amenability to laboratory experimentation and the availability of extensive genetic and germplasm resources. Considerable natural variation has been uncovered for a variety of traits including flowering time, vernalization responsiveness, and above-ground growth characteristics. However, cell wall composition differences remain underexplored. Therefore, we assessed cell wall-related traits relevant to biomass conversion to biofuels in seven Brachypodium inbred lines that were chosen based on their high level of genotypic diversity as well as available genome sequences and recombinant inbred line (RIL) populations. Senesced stems plus leaf sheaths from these lines exhibited significant differences in acetyl bromide soluble lignin (ABSL), cell wall polysaccharide-derived sugars, hydroxycinnamates content, and syringyl:guaiacyl:p-hydroxyphenyl (S:G:H) lignin ratios. Free glucose, sucrose, and starch content also differed significantly in senesced stems, as did the amounts of sugars released from cell wall polysaccharides (digestibility) upon exposure to a panel of thermochemical pretreatments followed by hydrolytic enzymatic digestion. Correlations were identified between inbred line lignin compositions and plant growth characteristics such as biomass accumulation and heading date (HD), and between amounts of cell wall polysaccharides and biomass digestibility. Finally, stem cell wall p-coumarate and ferulate contents and free-sugars content changed significantly with increased duration of vernalization for some inbred lines. Taken together, these results show that Brachypodium displays substantial phenotypic variation with respect to cell wall composition and biomass digestibility, with some compositional differences correlating with growth characteristics. Moreover, besides influencing HD and biomass accumulation, vernalization was found to affect cell wall composition and free sugars accumulation in some Brachypodium inbred lines, suggesting genetic differences in how vernalization affects carbon flux to polysaccharides. The availability of related RIL populations will allow for the genetic and molecular dissection of this natural variation, the knowledge of which may inform ways to genetically improve bioenergy crop grasses. C1 [Cass, Cynthia L.; Sedbrook, John C.] Illinois State Univ, Sch Biol Sci, Normal, IL 61761 USA. [Cass, Cynthia L.; Karlen, Steven D.; Smith, Rebecca A.; Ralph, John; Sedbrook, John C.] Univ Wisconsin, US DOE, Great Lakes Bioenergy Res Ctr, Madison, WI USA. [Lavell, Anastasiya A.; Garvin, David F.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN USA. [Lavell, Anastasiya A.; Garvin, David F.] USDA ARS, Plant Sci Res Ctr, St Paul, MN 55108 USA. [Santoro, Nicholas; Foster, Cliff E.] Michigan State Univ, US DOE, Great Lakes Bioenergy Res Ctr, E Lansing, MI 48824 USA. [Ralph, John] Univ Wisconsin, Dept Biochem, 420 Henry Mall, Madison, WI 53705 USA. RP Sedbrook, JC (reprint author), Illinois State Univ, Sch Biol Sci, Normal, IL 61761 USA.; Sedbrook, JC (reprint author), Univ Wisconsin, US DOE, Great Lakes Bioenergy Res Ctr, Madison, WI USA. EM jcsedbr@ilstu.edu FU Department of Energy's Great Lakes Bioenergy Research Center (Department of Energy, Biological and Environmental Research, Office of Science) [DE FCO2-07ER64494]; USDA-ARS CRIS Project [5062-21000-030-00D] FX This work was supported by the Department of Energy's Great Lakes Bioenergy Research Center (Department of Energy, Biological and Environmental Research, Office of Science grant no. DE FCO2-07ER64494), and by USDA-ARS CRIS Project 5062-21000-030-00D NR 54 TC 2 Z9 2 U1 7 U2 11 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD MAY 26 PY 2016 VL 7 AR 708 DI 10.3389/fpls.2016.00708 PG 16 WC Plant Sciences SC Plant Sciences GA DN0KR UT WOS:000376753300001 PM 27303415 ER PT J AU Zhang, YZ Lee, BR Du, WX Lyu, SC Nadeau, KC Grauke, LJ Zhang, Y Wang, S Fan, YT Yi, J McHugh, TH AF Zhang, Yuzhu Lee, BoRam Du, Wen-Xian Lyu, Shu-Chen Nadeau, Kari C. Grauke, Larry J. Zhang, Yan Wang, Shuo Fan, Yuting Yi, Jiang McHugh, Tara H. TI Identification and Characterization of a New Pecan [Carya illinoinensis (Wangenh.) K. Koch] Allergen, Car i 2 SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE allergy; X-ray crystallography; Western blot; Carya illinoinensis (Wangenh.) K. Koch ID MAJOR PEANUT ALLERGEN; SEED STORAGE PROTEIN; PINE PINUS-KORAIENSIS; ARA H 1; ANGSTROM RESOLUTION; MOLECULAR GRAPHICS; CRYSTAL-STRUCTURE; PROGRAM; VICILIN; FOOD AB The 7S vicilin and 11S legumin seed storage globulins belong to the cupin protein superfamily and are major food allergens in many foods from the "big eight" food allergen groups. Here, for the first time, pecan vicilin was found to be a food allergen. Western blot experiments revealed that 30% of 27 sera used in this study and 24% of the sera from 25 patients with double-blind, placebo controlled clinical pecan allergy contained IgE antibodies specific to pecan vicilin. This allergen consists of a low-complexity region at its N-terminal and a structured domain at the C-terminal that contains two cupin motifs and forms homotrimers. The crystal structure of recombinant pecan vicilin was determined. The refined structure gave R/R-free values of 0.218/0.262 for all data to 2.65 angstrom. There were two triineric biological units in the crystallographic asymmetric unit. Pecan vicilin is also a copper protein. These data may facilitate the understanding of the nutritional value and the allergenicity relevance of the copper binding property of seed storage proteins in tree nuts. C1 [Zhang, Yuzhu; Lee, BoRam; Du, Wen-Xian; Fan, Yuting; McHugh, Tara H.] ARS, Western Reg Res Ctr, Pacific West Area, USDA, 800 Buchanan St, Albany, CA 94710 USA. [Lyu, Shu-Chen; Nadeau, Kari C.] Stanford Univ, Sch Med, Dept Pediat, Div Pediat Immunol Allergy & Rheumatol, 269 Campus Dr, Stanford, CA 94305 USA. [Grauke, Larry J.] USDA ARS SPA, Crop Germplasm Res, Southern Plains Agr Res Ctr, 2881 F&B Rd, College Stn, TX 77845 USA. [Zhang, Yan; Wang, Shuo] Tianjin Univ Sci & Technol, Minist Educ, Key Lab Food Nutr & Safety, Tianjin 300222, Peoples R China. [Fan, Yuting] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China. [Yi, Jiang] Shenzhen Univ, Coll Chem & Environm Engn, Shenzhen 518060, Peoples R China. [Lee, BoRam] Univ Texas SW Med Ctr Dallas, Div Basic Sci, Dallas, TX 75390 USA. RP Zhang, YZ (reprint author), ARS, Western Reg Res Ctr, Pacific West Area, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM yuzhu.zhang@ars.usda.gov FU U.S. Department of Agriculture-Agricultural Research Service; Sean N. Parker Center for Allergy and Asthma Research at Stanford University; National Institutes of Health, National Institute of General Medical Sciences; Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy [DE-AC02-05CH11231] FX This research was supported by funds from the U.S. Department of Agriculture-Agricultural Research Service and by the Sean N. Parker Center for Allergy and Asthma Research at Stanford University. The Berkeley Center for Structural Biology was supported in part by the National Institutes of Health, National Institute of General Medical Sciences. The Advanced Light Source was supported by the Director, Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy, under Contract No. DE-AC02-05CH11231. NR 36 TC 1 Z9 1 U1 4 U2 12 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAY 25 PY 2016 VL 64 IS 20 BP 4146 EP 4151 DI 10.1021/acs.jafc.6b00884 PG 6 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DN1KV UT WOS:000376825600025 PM 27128197 ER PT J AU Brunelle, BW O'Grady, AM Nicholson, EM Seabury, CM AF Brunelle, Brian W. O'Grady, Allison M. Nicholson, Eric M. Seabury, Christopher M. TI Disparate Modes of Evolution Shaped Modern Prion (PRNP) and Prion-Related Doppel (PRND) Variation in Domestic Cattle SO PLOS ONE LA English DT Article ID PROTEIN-PROTEIN INTERACTIONS; STRONG PURIFYING SELECTION; STATISTICAL-METHOD; SIMPLE DEPENDENCE; DNA POLYMORPHISM; DEATH EVOLUTION; DEFICIENT MICE; GENE FAMILY; KNOCKOUT; EXPRESSION AB Previous investigations aimed at determining whether the mammalian prion protein actually facilitates tangible molecular aspects of either a discrete or pleiotropic functional niche have been debated, especially given the apparent absence of overt behavioral or physiological phenotypes associated with several mammalian prion gene (PRNP) knockout experiments. Moreover, a previous evaluation of PRNP knockout cattle concluded that they were normal, suggesting that the bovine prion protein is physiologically dispensable. Herein, we examined the frequency and distribution of nucleotide sequence variation within the coding regions of bovine PRNP and the adjacent Doppel (PRND) gene, a proximal paralogue to PRNP on BTA13. Evaluation of PRND variation demonstrated that the gene does not depart from a strictly neutral model of molecular evolution, and would therefore not be expected to influence tests of selection within PRNP. Collectively, our analyses confirm that intense purifying selection is indeed occurring directly on bovine PRNP, which is indicative of a protein with an important role. These results suggest that the lack of observed fitness effects may not manifest in the controlled environmental conditions used to care for and raise PRNP knockout animals. C1 [Brunelle, Brian W.; O'Grady, Allison M.; Nicholson, Eric M.] ARS, Virus & Pr Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Seabury, Christopher M.] Texas A&M Univ, Coll Vet Med, Dept Vet Pathobiol, College Stn, TX 77843 USA. [Brunelle, Brian W.] ARS, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. RP Nicholson, EM (reprint author), ARS, Virus & Pr Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA.; Seabury, CM (reprint author), Texas A&M Univ, Coll Vet Med, Dept Vet Pathobiol, College Stn, TX 77843 USA. EM Eric.Nicholson@ars.usda.gov; CSeabury@cvm.tamu.edu FU USDA-ARS FX This work was funded in its entirety by congressional appropriation to the USDA-ARS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 60 TC 0 Z9 0 U1 1 U2 1 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 25 PY 2016 VL 11 IS 5 AR e0155924 DI 10.1371/journal.pone.0155924 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DN2GI UT WOS:000376881700041 PM 27224046 ER PT J AU Chen, MS Liu, SZ Wang, HY Cheng, XY El Bouhssini, M Whitworth, RJ AF Chen, Ming-Shun Liu, Sanzhen Wang, Haiyan Cheng, Xiaoyan El Bouhssini, Mustapha Whitworth, R. Jeff TI Massive Shift in Gene Expression during Transitions between Developmental Stages of the Gall Midge, Mayetiola Destructor SO PLOS ONE LA English DT Article ID HESSIAN FLY DIPTERA; WINTER-WHEAT; CECIDOMYIIDAE POPULATIONS; VIRULENCE; CLONING; TRANSCRIPTOME; REVEALS; CDNAS; RESISTANCE; PROTEINS AB Mayetiola destructor is a destructive pest of wheat and has six developmental stages. Molecular mechanisms controlling the transition between developmental stages remain unknown. Here we analyzed genes that were expressed differentially between two successive developmental stages, including larvae at 1, 3, 5, and 7 days, pupae, and adults. A total of 17,344 genes were expressed during one or more of these studied stages. Among the expressed genes, 38-68% were differently expressed between two successive stages, with roughly equal percentages of up-and down-regulated genes. Analysis of the functions of the differentially expressed genes revealed that each developmental stage had some unique types of expressed genes that are characteristic of the physiology at that stage. This is the first genome-wide analysis of genes differentially expressed in different stages in a gall midge. The large dataset of up-and down-regulated genes in each stage of the insect shall be very useful for future research to elucidate mechanisms regulating insect development and other biological processes. C1 [Chen, Ming-Shun] Kansas State Univ, USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Chen, Ming-Shun; Cheng, Xiaoyan; Whitworth, R. Jeff] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Liu, Sanzhen] Kansas State Univ, Dept Plant Pathol, Throckmorton Hall, Manhattan, KS 66506 USA. [Wang, Haiyan] Kansas State Univ, Dept Stat, Manhattan, KS 66506 USA. [El Bouhssini, Mustapha] Int Ctr Agr Res Dry Area, Rabat, Morocco. RP Chen, MS (reprint author), Kansas State Univ, USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA.; Chen, MS (reprint author), Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. EM mchen@ksu.edu FU U.S. Department of Agriculture [USDA NIFA 2010-03741]; Agricultural Research Service, the U.S. Department of Agriculture FX The research was supported by a grant from the U.S. Department of Agriculture (USDA NIFA 2010-03741) and by annual base funds to MSC from the Agricultural Research Service, the U.S. Department of Agriculture. NR 50 TC 1 Z9 1 U1 6 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 25 PY 2016 VL 11 IS 5 AR e0155616 DI 10.1371/journal.pone.0155616 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DN2GI UT WOS:000376881700024 PM 27224654 ER PT J AU Keiter, DA Cunningham, FL Rhodes, OE Irwin, BJ Beasley, JC AF Keiter, David A. Cunningham, Fred L. Rhodes, Olin E., Jr. Irwin, Brian J. Beasley, James C. TI Optimization of Scat Detection Methods for a Social Ungulate, the Wild Pig, and Experimental Evaluation of Factors Affecting Detection of Scat SO PLOS ONE LA English DT Article ID BOAR SUS-SCROFA; POPULATION-SIZE; SOUTH-CAROLINA; FECAL DNA; DEER; SELECTION; GENETICS; IMPACT; RANGE; FECES AB Collection of scat samples is common in wildlife research, particularly for genetic capture-mark-recapture applications. Due to high degradation rates of genetic material in scat, large numbers of samples must be collected to generate robust estimates. Optimization of sampling approaches to account for taxa-specific patterns of scat deposition is, therefore, necessary to ensure sufficient sample collection. While scat collection methods have been widely studied in carnivores, research to maximize scat collection and noninvasive sampling efficiency for social ungulates is lacking. Further, environmental factors or scat morphology may influence detection of scat by observers. We contrasted performance of novel radial search protocols with existing adaptive cluster sampling protocols to quantify differences in observed amounts of wild pig (Sus scrofa) scat. We also evaluated the effects of environmental (percentage of vegetative ground cover and occurrence of rain immediately prior to sampling) and scat characteristics (fecal pellet size and number) on the detectability of scat by observers. We found that 15- and 20-m radial search protocols resulted in greater numbers of scats encountered than the previously used adaptive cluster sampling approach across habitat types, and that fecal pellet size, number of fecal pellets, percent vegetative ground cover, and recent rain events were significant predictors of scat detection. Our results suggest that use of a fixed-width radial search protocol may increase the number of scats detected for wild pigs, or other social ungulates, allowing more robust estimation of population metrics using noninvasive genetic sampling methods. Further, as fecal pellet size affected scat detection, juvenile or smaller-sized animals may be less detectable than adult or large animals, which could introduce bias into abundance estimates. Knowledge of relationships between environmental variables and scat detection may allow researchers to optimize sampling protocols to maximize utility of noninvasive sampling for wild pigs and other social ungulates. C1 [Keiter, David A.; Rhodes, Olin E., Jr.; Beasley, James C.] Univ Georgia, Savannah River Ecol Lab, Aiken, SC USA. [Keiter, David A.; Beasley, James C.] Univ Georgia, DB Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. [Cunningham, Fred L.] US Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, USDA, Starkville, MS USA. [Rhodes, Olin E., Jr.] Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. [Irwin, Brian J.] Univ Georgia, DB Warnell Sch Forestry & Nat Resources, Georgia Cooperat Fish & Wildlife Res Unit, US Geol Survey, Athens, GA 30602 USA. RP Keiter, DA (reprint author), Univ Georgia, Savannah River Ecol Lab, Aiken, SC USA.; Keiter, DA (reprint author), Univ Georgia, DB Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. EM david.keiter@gmail.com FU United States Department of Energy [DE-FC09-07SR22506]; United States Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center; U.S. Geological Survey; Georgia Department of Natural Resources; U.S. Fish and Wildlife Service; University of Georgia; Wildlife Management Institute; United States Government FX Funding for this research was provided by the United States Department of Energy under Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation and the United States Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center (https://www.aphis.usda.gov/wps/portal/aphis/ourfocus/wildlifedamage?uri le=wcm%3Apath%3A%2FAPHIS_Content_Library%2FSA_Our_Focus%2FSA_Wildlife_Da mage%2FSA_Programs%2FSA_NWRC). Researchers at the USDA-APHIS National Wildlife Research Center participated in design of this study and preparation of the manuscript.; We thank the volunteers in the fall 2014 Wildlife Techniques course at the University of Georgia who assisted with this research and L. Lee for her photograph of bottomland hardwood habitat at the Savannah River Site. All other photographs were provided by D. Keiter. The Georgia Cooperative Fish and Wildlife Research Unit is sponsored jointly by the U.S. Geological Survey, the Georgia Department of Natural Resources, the U.S. Fish and Wildlife Service, the University of Georgia, and the Wildlife Management Institute. This paper was prepared as an account of work sponsored by an agency of the United States Government. Neither the U.S. Department of Agriculture, nor any of its employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. The views and opinions of authors expressed herein do not necessarily state or reflect those of the U.S. Department of Agriculture. Although these data have been processed successfully on a computer system at the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data or associated code on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. The USGS or the U.S. Government shall not be held liable for improper or incorrect use of the data or code described and/or contained herein. NR 43 TC 0 Z9 0 U1 7 U2 14 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 25 PY 2016 VL 11 IS 5 AR e0155615 DI 10.1371/journal.pone.0155615 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DN2GI UT WOS:000376881700023 PM 27224453 ER PT J AU Wolf, S Keenan, TF Fisher, JB Baldocchi, DD Desai, AR Richardson, AD Scott, RL Law, BE Litvak, ME Brunsell, NA Peters, W van der Laan-Luijkx, IT AF Wolf, Sebastian Keenan, Trevor F. Fisher, Joshua B. Baldocchi, Dennis D. Desai, Ankur R. Richardson, Andrew D. Scott, Russell L. Law, Beverly E. Litvak, Marcy E. Brunsell, Nathaniel A. Peters, Wouter van der Laan-Luijkx, Ingrid T. TI Warm spring reduced carbon cycle impact of the 2012 US summer drought SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE seasonal climate anomalies; carbon uptake; ecosystem fluxes; biosphere; atmosphere feedbacks; eddy covariance ID EDDY-COVARIANCE; SOIL-MOISTURE; CLIMATE; FOREST; REDUCTION; EXCHANGE; DIOXIDE; HEAT; PREDICTABILITY; FEEDBACKS AB The global terrestrial carbon sink offsets one-third of the world's fossil fuel emissions, but the strength of this sink is highly sensitive to large-scale extreme events. In 2012, the contiguous United States experienced exceptionally warm temperatures and the most severe drought since the Dust Bowl era of the 1930s, resulting in substantial economic damage. It is crucial to understand the dynamics of such events because warmer temperatures and a higher prevalence of drought are projected in a changing climate. Here, we combine an extensive network of direct ecosystem flux measurements with satellite remote sensing and atmospheric inverse modeling to quantify the impact of the warmer spring and summer drought on biosphere-atmosphere carbon and water exchange in 2012. We consistently find that earlier vegetation activity increased spring carbon uptake and compensated for the reduced uptake during the summer drought, which mitigated the impact on net annual carbon uptake. The early phenological development in the Eastern Temperate Forests played a major role for the continental-scale carbon balance in 2012. The warm spring also depleted soilwater resources earlier, and thus exacerbated water limitations during summer. Our results show that the detrimental effects of severe summer drought on ecosystem carbon storage can be mitigated by warming-induced increases in spring carbon uptake. However, the results also suggest that the positive carbon cycle effect of warm spring enhances water limitations and can increase summer heating through biosphere-atmosphere feedbacks. C1 [Wolf, Sebastian; Baldocchi, Dennis D.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Wolf, Sebastian] ETH, Dept Environm Syst Sci, CH-8092 Zurich, Switzerland. [Keenan, Trevor F.] Macquarie Univ, Dept Biol Sci, Sydney, NSW 2109, Australia. [Fisher, Joshua B.] CALTECH, Jet Prop Lab, 4800 Oak Grove Dr, Pasadena, CA 91109 USA. [Desai, Ankur R.] Univ Wisconsin, Atmospher & Ocean Sci, Madison, WI 53706 USA. [Richardson, Andrew D.] Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA. [Scott, Russell L.] USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ 85719 USA. [Law, Beverly E.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Litvak, Marcy E.] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. [Brunsell, Nathaniel A.] Univ Kansas, Dept Geog, Lawrence, KS 66045 USA. [Peters, Wouter; van der Laan-Luijkx, Ingrid T.] Wageningen Univ, Dept Meteorol & Air Qual, NL-6708 PB Wageningen, Netherlands. [Peters, Wouter] Univ Groningen, Ctr Isotope Res, NL-9747 AG Groningen, Netherlands. [Keenan, Trevor F.] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Earth & Environm Sci, Berkeley, CA 94720 USA. RP Wolf, S (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA.; Wolf, S (reprint author), ETH, Dept Environm Syst Sci, CH-8092 Zurich, Switzerland. EM sewolf@ethz.ch RI van der Laan-Luijkx, Ingrid/G-9169-2011; Wolf, Sebastian/B-4580-2010; Keenan, Trevor/B-2744-2010; Baldocchi, Dennis/A-1625-2009; Law, Beverly/G-3882-2010; OI van der Laan-Luijkx, Ingrid/0000-0002-3990-6737; Wolf, Sebastian/0000-0001-7717-6993; Keenan, Trevor/0000-0002-3347-0258; Baldocchi, Dennis/0000-0003-3496-4919; Law, Beverly/0000-0002-1605-1203; Desai, Ankur/0000-0002-5226-6041; Fisher, Joshua/0000-0003-4734-9085 FU Carbon Dioxide Information Analysis Center at the Oak Ridge National Laboratory; European Commission [300083]; US Department of Energy's Office of Science [DE-AC02-05CH11231]; Macquarie University Research Fellowship; National Aeronautics and Space Administration; NASA's Terrestrial Hydrology Program; National Science Foundation (NSF), through the Macrosystems Biology program [EF-1065029]; LTER program [DEB-1114804]; NASA ROSES [0486V-874F]; NSF EPSCoR program [EPS-0553722, EPS-0919443]; LTER program at the Konza Prairie Biological Station [DEB-0823341]; NWO [SH-060-13]; OCW/NWO for ICOS-NL FX We acknowledge support from the Carbon Dioxide Information Analysis Center at the Oak Ridge National Laboratory, particularly B. Yang. We thank D. M. Ricciuto for assistance with the gap-filling of climate data and M. Sikka for remote-sensing data processing. We thank all data contributors for this synthesis study, particularly P. Blanken, G. Bohrer, D. Bowling, S. Burns, K. L. Clark, D. Hollinger, S. Ma, Q. Mu, K. A. Novick, S. A. Papuga, F. Rahman, and M. Zhao. We thank K. A. Novick, G. Bohrer, E. van Gorsel, and E. Paul-Limoges for helpful comments on the manuscript. We also appreciate the constructive comments of the reviewers and the editor. This research was supported by the European Commission's FP7 Marie Curie International Outgoing Fellowship Grant 300083 (to S.W.). Funding for the AmeriFlux Management Project was provided by the US Department of Energy's Office of Science (Contract DE-AC02-05CH11231). T.F.K. acknowledges support from a Macquarie University Research Fellowship. J.B.F. carried out the research at the Jet Propulsion Laboratory, California Institute of Technology, under a contract with the National Aeronautics and Space Administration, and acknowledges support from NASA's Terrestrial Hydrology Program. A.D.R. acknowledges support from the National Science Foundation (NSF), through the Macrosystems Biology program (Award EF-1065029) and the LTER program (DEB-1114804). M. E. L. acknowledges support from NASA ROSES (Award 0486V-874F). N.A.B. acknowledges support from the NSF EPSCoR program (EPS-0553722 and EPS-0919443) and the LTER program at the Konza Prairie Biological Station (DEB-0823341). W. P. and I.T.v.d.L.-L. acknowledge funding from NWO (SH-060-13) for computing time. I.T.v.d.L.-L. received financial support from OCW/NWO for ICOS-NL. NR 39 TC 9 Z9 9 U1 19 U2 49 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAY 24 PY 2016 VL 113 IS 21 BP 5880 EP 5885 DI 10.1073/pnas.1519620113 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DN0UD UT WOS:000376779900048 PM 27114518 ER PT J AU Collier, R Bragg, J Hernandez, BT Vogel, JP Thilmony, R AF Collier, Ray Bragg, Jennifer Hernandez, Bryan T. Vogel, John P. Thilmony, Roger TI Use of Agrobacterium rhizogenes Strain 18r12v and Paromomycin Selection for Transformation of Brachypodium distachyon and Brachypodium sylvaticum SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE transformation; Brachypodium; Agrobacterium rhizogenes; paromomycin; tissue culture; callus ID SELECTABLE MARKER GENES; MEDIATED TRANSFORMATION; PLANT TRANSFORMATION; PARTICLE BOMBARDMENT; POLYUBIQUITIN GENES; PROMOTER ACTIVITY; EXPRESSION; L.; ELECTROPORATION; PROTOPLASTS AB The genetic transformation of monocot grasses is a resource intensive process, the quality and efficiency of which is dependent in part upon the method of DNA introduction, as well as the ability to effectively separate transformed from wildtype tissue. Agrobacterium-mediated transformation of Brachypodium has relied mainly on Agrobacterium tumefaciens strain AGL1. Currently the antibiotic hygromycin B has been the selective agent of choice for robust identification of transgenic calli in Brachypodium distachyon and Brachypodium sylvaticum but few other chemicals have been shown to work as well for selection of transgenic Brachypodium cells in tissue culture. This study demonstrates that Agrobacterium rhizogenes strain 18r12v and paromomycin selection can be successfully used for the efficient generation of transgenic B. distachyon and B. sylvaticurn. Additionally we observed that the transformation rates were similar to or higher than those obtained with A. turnefaciens strain AGL1 and hygromycin selection. The A. rhizogenes strain 18r12v harboring the pARS1 binary vector and paromomycin selection is an effective means of generating transgenic Brachypodium plants. This novel approach will facilitate the transgenic complementation of T-DNA knockout mutants of B. distachyon which were created using hygromycin selection, as well as aid the implementation of more complex genome manipulation strategies which require multiple rounds of transformation. C1 [Collier, Ray; Bragg, Jennifer; Vogel, John P.; Thilmony, Roger] USDA ARS, Crop Improvement & Genet Res Unit, Western Reg Res Ctr, Albany, CA USA. [Hernandez, Bryan T.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Vogel, John P.] Joint Genome Inst, Dept Energy, Walnut Creek, CA USA. RP Thilmony, R (reprint author), USDA ARS, Crop Improvement & Genet Res Unit, Western Reg Res Ctr, Albany, CA USA. EM roger.thilmony@ars.usda.gov FU USDA Agricultural Research Service CRIS project [2030-21000-020-00D]; Office of Biological and Environmental Research, Office of Science, US Department of Energy [DE-SC0001526]; U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility [DE-AC02-05CH11231] FX RC, BH, and RT were supported by USDA Agricultural Research Service CRIS project 2030-21000-020-00D. JB and JV were supported by Office of Biological and Environmental Research, Office of Science, US Department of Energy, interagency agreement DE-SC0001526. The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported under Contract No. DE-AC02-05CH11231. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 32 TC 0 Z9 0 U1 7 U2 12 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD MAY 24 PY 2016 VL 7 AR 716 DI 10.3389/fpls.2016.00716 PG 9 WC Plant Sciences SC Plant Sciences GA DN0HD UT WOS:000376744100001 PM 27252729 ER PT J AU Satterlee, T Cary, JW Calvo, AM AF Satterlee, Timothy Cary, Jeffrey W. Calvo, Ana M. TI RmtA, a Putative Arginine Methyltransferase, Regulates Secondary Metabolism and Development in Aspergillus flavus SO PLOS ONE LA English DT Article ID FUNGAL DEVELOPMENT; AFLATOXIN BIOSYNTHESIS; SCLEROTIAL PRODUCTION; CONIDIOPHORE DEVELOPMENT; MYCOTOXIN PRODUCTION; SEXUAL DEVELOPMENT; IN-VIVO; GENE; NIDULANS; COMPLEX AB Aspergillus flavus colonizes numerous oil seed crops such as corn, peanuts, treenuts and cotton worldwide, contaminating them with aflatoxin and other harmful potent toxins. In the phylogenetically related model fungus Aspergillus nidulans, the methyltransferase, RmtA, has been described to be involved in epigenetics regulation through histone modification. Epigenetics regulation affects a variety of cellular processes, including morphogenesis and secondary metabolism. Our study shows that deletion of rmtA in A. flavus results in hyperconidiating colonies, indicating that rmtA is a repressor of asexual development in this fungus. The increase in conidiation in the absence of rmtA coincides with greater expression of brlA, abaA, and wetA compared to that in the wild type. Additionally, the rmtA deletion mutant presents a drastic reduction or loss of sclerotial production, while forced expression of this gene increased the ability of this fungus to generate these resistant structures, revealing rmtA as a positive regulator of sclerotial formation. Importantly, rmtA is also required for the production of aflatoxin B-1 in A. flavus, affecting the expression of aflJ. Furthermore, biosynthesis of additional metabolites is also controlled by rmtA, indicating a broad regulatory output in the control of secondary metabolism. This study also revealed that rmtA positively regulates the expression of the global regulatory gene veA, which could contribute to mediate the effects of rmtA on development and secondary metabolism in this relevant opportunistic plant pathogen. C1 [Satterlee, Timothy; Calvo, Ana M.] No Illinois Univ, Dept Biol Sci, De Kalb, IL 60115 USA. [Cary, Jeffrey W.] USDA ARS, So Reg Res Ctr, POB 19687, New Orleans, LA 70124 USA. RP Calvo, AM (reprint author), No Illinois Univ, Dept Biol Sci, De Kalb, IL 60115 USA. EM amcalvo@niu.edu FU United States Department of Agriculture [58-6435-4-015 AMC]; Department of Biological Sciences at Northern Illinois University FX This work was supported by the United States Department of Agriculture grant 58-6435-4-015 AMC.; This work was supported by USDA grant 58-6435-4-015 and the Department of Biological Sciences at Northern Illinois University. NR 61 TC 0 Z9 0 U1 9 U2 11 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 23 PY 2016 VL 11 IS 5 AR e0155575 DI 10.1371/journal.pone.0155575 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DN2FT UT WOS:000376880200017 PM 27213959 ER PT J AU Zaidi, SSEA Shafiq, M Amin, I Scheffler, BE Scheffler, JA Briddon, RW Mansoor, S AF Zaidi, Syed Shan-e-Ali Shafiq, Muhammad Amin, Imran Scheffler, Brian E. Scheffler, Jodi A. Briddon, Rob W. Mansoor, Shahid TI Frequent Occurrence of Tomato Leaf Curl New Delhi Virus in Cotton Leaf Curl Disease Affected Cotton in Pakistan SO PLOS ONE LA English DT Article ID PCR-MEDIATED AMPLIFICATION; YELLOW VEIN DISEASE; DNA-BETA COMPLEXES; BIPARTITE BEGOMOVIRUS; NEW-WORLD; MONOPARTITE BEGOMOVIRUSES; UNIVERSAL PRIMERS; DISTINCT STRAIN; BUREWALA VIRUS; 1ST REPORT AB Cotton leaf curl disease (CLCuD) is the major biotic constraint to cotton production on the Indian subcontinent, and is caused by monopartite begomoviruses accompanied by a specific DNA satellite, Cotton leaf curl Multan betasatellite (CLCuMB). Since the breakdown of resistance against CLCuD in 2001/2002, only one virus, the "Burewala" strain of Cotton leaf curl Kokhran virus (CLCuKoV-Bur), and a recombinant form of CLCuMB have consistently been identified in cotton across the major cotton growing areas of Pakistan. Unusually a bipartite isolate of the begomovirus Tomato leaf curl virus was identified in CLCuD-affected cotton recently. In the study described here we isolated the bipartite begomovirus Tomato leaf curl New Delhi virus (ToLCNDV) from CLCuD-affected cotton. To assess the frequency and geographic occurrence of ToLCNDV in cotton, CLCuD-symptomatic cotton plants were collected from across the Punjab and Sindh provinces between 2013 and 2015. Analysis of the plants by diagnostic PCR showed the presence of CLCuKoV-Bur in all 31 plants examined and ToLCNDV in 20 of the samples. Additionally, a quantitative real-time PCR analysis of the levels of the two viruses in co-infected plants suggests that coinfection of ToLCNDV with the CLCuKoV-Bur/CLCuMB complex leads to an increase in the levels of CLCuMB, which encodes the major pathogenicity (symptom) determinant of the complex. The significance of these results are discussed. C1 [Zaidi, Syed Shan-e-Ali; Shafiq, Muhammad; Amin, Imran; Briddon, Rob W.; Mansoor, Shahid] Natl Inst Biotechnol & Genet Engn, Agr Biotechnol Div, POB 577,Jhang Rd, Faisalabad, Pakistan. [Zaidi, Syed Shan-e-Ali; Shafiq, Muhammad] PIEAS, Islamabad, Pakistan. [Scheffler, Brian E.] Genom & Bioinformat Res Unit, 141 Expt Stn Rd, Stoneville, MS 38776 USA. [Scheffler, Jodi A.] USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. RP Mansoor, S (reprint author), Natl Inst Biotechnol & Genet Engn, Agr Biotechnol Div, POB 577,Jhang Rd, Faisalabad, Pakistan. EM shahidmansoor7@gmail.com RI Briddon, Robert/I-5921-2012; OI Briddon, Robert/0000-0001-8738-0365; Zaidi, Syed Shan e Ali/0000-0002-2976-4624; Scheffler, Brian/0000-0003-1968-8952 FU Higher Education Commission (HEC), Govt. of Pakistan; International Center for Agricultural Research in the Dry Areas (ICARDA) - United States Department of Agriculture (USDA), Agricultural Research Service (ARS) [58-6402-0-178F] FX SSZ is supported by Ph.D scholarship from Higher Education Commission (HEC), Govt. of Pakistan under "Indigenous 5000 Ph.D Fellowship Program". This research was funded in part through the "Pak-US cotton productivity enhancement program'' of the International Center for Agricultural Research in the Dry Areas (ICARDA) funded by United States Department of Agriculture (USDA), Agricultural Research Service (ARS), under Agreement No. 58-6402-0-178F. Any opinions, findings, conclusions or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the USDA or ICARDA. NR 55 TC 2 Z9 2 U1 11 U2 13 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 23 PY 2016 VL 11 IS 5 AR e0155520 DI 10.1371/journal.pone.0155520 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DN2FT UT WOS:000376880200013 PM 27213535 ER PT J AU Qiu, H Cai, GH Luo, J Bhattacharya, D Zhang, N AF Qiu, Huan Cai, Guohong Luo, Jing Bhattacharya, Debashish Zhang, Ning TI Extensive horizontal gene transfers between plant pathogenic fungi SO BMC BIOLOGY LA English DT Article DE Horizontal gene transfer; Pathogenesis; Magnaporthales; Colletotrichum; Carbohydrate activating enzymes ID MULTIPLE SEQUENCE ALIGNMENT; CARBOHYDRATE-ACTIVE ENZYMES; PHYLOGENETIC ANALYSES; EVOLUTION; GENOME; DATABASE; LIFE; TREE; TOOL; CLASSIFICATION AB Background: Horizontal gene transfer (HGT) plays an important role in the adaptation of lineages to changing environments. The extent of this process in eukaryotes, however, remains controversial. The most well-known and dramatic form of HGT represents intracellular gene transfer from endosymbionts to the host nuclear genome. Such episodes of transfer typically involve hundreds of genes and are thought to be possible only in the case of endosymbiosis. Results: Using a conservative phylogenomic approach, we analyzed genomic data from the fungal pathogen Magnaporthiopsis incrustans in the order Magnaporthales and identified two instances of exclusive sharing of HGT-derived gene markers between Magnaporthales and another lineage of plant-pathogenic fungi in the genus Colletotrichum. Surprisingly, inspection of these data demonstrated that HGT is far more widespread than anticipated, with more than 90 genes (including 33 highly supported candidates) being putatively transferred between Magnaporthales and Colletotrichum. These gene transfers are often physically linked in the genome and show more than two-fold functional enrichment in carbohydrate activating enzymes associated with plant cell wall degradation. Conclusions: Our work provides a novel perspective on the scale of HGT between eukaryotes. These results challenge the notion that recognized HGT plays a minor role in the evolution of fungal lineages, and in the case we describe, is likely implicated in the evolution of plant pathogenesis. More generally, we suggest that the expanding database of closely related eukaryotic genomes and the application of novel analytic methods will further underline the significant impact of foreign gene acquisition across the tree of life. Major lifestyle transitions such as those accompanying the origin of extremophily or pathogenesis are expected to be ideal candidates for studying the mode and tempo of HGT. C1 [Qiu, Huan; Bhattacharya, Debashish] Rutgers State Univ, Dept Ecol Evolut & Nat Resources, Foran Hall,59 Dudley Rd, New Brunswick, NJ 08901 USA. [Luo, Jing; Zhang, Ning] Rutgers State Univ, Dept Bot & Plant Pathol, Foran Hall 201,59 Dudley Rd, New Brunswick, NJ 08901 USA. [Cai, Guohong] USDA, Natl Anim Dis Ctr, 1920 Dayton Ave,POB 70, Ames, IA 50010 USA. [Zhang, Ning] Rutgers State Univ, Dept Biochem & Microbiol, 76 Lipman Dr, New Brunswick, NJ 08901 USA. RP Qiu, H (reprint author), Rutgers State Univ, Dept Ecol Evolut & Nat Resources, Foran Hall,59 Dudley Rd, New Brunswick, NJ 08901 USA.; Zhang, N (reprint author), Rutgers State Univ, Dept Bot & Plant Pathol, Foran Hall 201,59 Dudley Rd, New Brunswick, NJ 08901 USA.; Zhang, N (reprint author), Rutgers State Univ, Dept Biochem & Microbiol, 76 Lipman Dr, New Brunswick, NJ 08901 USA. EM huan.qiu.bio@gmail.com; zhang@aesop.rutgers.edu RI Zhang, Ning/K-3046-2012 OI Zhang, Ning/0000-0003-0755-2505 FU National Science Foundation of the United States [DEB 1145174, 1452971] FX The research was financially supported by grants from the National Science Foundation of the United States (DEB 1145174 and 1452971). NR 47 TC 2 Z9 2 U1 8 U2 18 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1741-7007 J9 BMC BIOL JI BMC Biol. PD MAY 23 PY 2016 VL 14 AR 41 DI 10.1186/s12915-016-0264-3 PG 11 WC Biology SC Life Sciences & Biomedicine - Other Topics GA DM3GW UT WOS:000376235900001 PM 27215567 ER PT J AU Grant, EHC Miller, DAW Schmidt, BR Adams, MJ Amburgey, SM Chambert, T Cruickshank, SS Fisher, RN Green, DM Hossack, BR Johnson, PTJ Joseph, MB Rittenhouse, TAG Ryan, ME Waddle, JH Walls, SC Bailey, LL Fellers, GM Gorman, TA Ray, AM Pilliod, DS Price, SJ Saenz, D Sadinski, W Muths, E AF Grant, Evan H. Campbell Miller, David A. W. Schmidt, Benedikt R. Adams, Michael J. Amburgey, Staci M. Chambert, Thierry Cruickshank, Sam S. Fisher, Robert N. Green, David M. Hossack, Blake R. Johnson, Pieter T. J. Joseph, Maxwell B. Rittenhouse, Tracy A. G. Ryan, Maureen E. Waddle, J. Hardin Walls, Susan C. Bailey, Larissa L. Fellers, Gary M. Gorman, Thomas A. Ray, Andrew M. Pilliod, David S. Price, Steven J. Saenz, Daniel Sadinski, Walt Muths, Erin TI Quantitative evidence for the effects of multiple drivers on continental-scale amphibian declines SO SCIENTIFIC REPORTS LA English DT Article ID POPULATION DECLINES; CONSERVATION BIOLOGY; ADAPTIVE MANAGEMENT; CLIMATE-CHANGE; LAND-USE; BIODIVERSITY; DYNAMICS; CHYTRIDIOMYCOSIS; EXTINCTIONS; OCCUPANCY AB Since amphibian declines were first proposed as a global phenomenon over a quarter century ago, the conservation community has made little progress in halting or reversing these trends. The early search for a "smoking gun" was replaced with the expectation that declines are caused by multiple drivers. While field observations and experiments have identified factors leading to increased local extinction risk, evidence for effects of these drivers is lacking at large spatial scales. Here, we use observations of 389 time-series of 83 species and complexes from 61 study areas across North America to test the effects of 4 of the major hypothesized drivers of declines. While we find that local amphibian populations are being lost from metapopulations at an average rate of 3.79% per year, these declines are not related to any particular threat at the continental scale; likewise the effect of each stressor is variable at regional scales. This result - that exposure to threats varies spatially, and populations vary in their response - provides little generality in the development of conservation strategies. Greater emphasis on local solutions to this globally shared phenomenon is needed. C1 [Grant, Evan H. Campbell] US Geol Survey, Patuxent Wildlife Res Ctr, SO Conte Anadromous Fish Lab, 1 Migratory Way, Turners Falls, MA 01376 USA. [Miller, David A. W.; Amburgey, Staci M.; Chambert, Thierry] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [Schmidt, Benedikt R.; Cruickshank, Sam S.] Univ Zurich, Dept Evolutionary Biol & Environm Studies, CH-8006 Zurich, Switzerland. [Schmidt, Benedikt R.] KARCH, CH-2000 Neuchatel, Switzerland. [Adams, Michael J.] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Corvallis, OR 97331 USA. [Chambert, Thierry] US Geol Survey, Patuxent Wildlife Res Ctr, Laurel, MD 20708 USA. [Fisher, Robert N.] US Geol Survey, Western Ecol Res Ctr, San Diego, CA 92101 USA. [Green, David M.] McGill Univ, Redpath Museum, 859 Sherbrooke St W, Montreal, PQ H3A 2K6, Canada. [Hossack, Blake R.] US Geol Survey, Northern Rocky Mt Sci Ctr, Aldo Leopold Wilderness Res Inst, Missoula, MT 59801 USA. [Johnson, Pieter T. J.; Joseph, Maxwell B.] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. [Rittenhouse, Tracy A. G.] Univ Connecticut, Dept Nat Resources & Environm, Storrs, CT 06269 USA. [Ryan, Maureen E.] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Waddle, J. Hardin] US Geol Survey, Wetland & Aquat Res Ctr, Lafayette, LA 70506 USA. [Walls, Susan C.] US Geol Survey, Wetland & Aquat Res Ctr, Gainesville, FL 32653 USA. [Bailey, Larissa L.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Fellers, Gary M.] US Geol Survey, Western Ecol Res Ctr, Point Reyes Stn, CA 94956 USA. [Gorman, Thomas A.] Virginia Tech, Dept Fish & Wildlife Conservat, Blacksburg, VA 24061 USA. [Ray, Andrew M.] Natl Pk Serv, Greater Yellowstone Network Program, Bozeman, MT 59715 USA. [Pilliod, David S.] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Boise, ID 83702 USA. [Price, Steven J.] Univ Kentucky, Dept Forestry, Lexington, KY 40506 USA. [Saenz, Daniel] Forest Serv, USDA, Southern Res Stn, Nacogdoches, TX 75961 USA. [Sadinski, Walt] US Geol Survey, Upper Midwest Environm Sci Ctr, La Crosse, WI 54603 USA. [Muths, Erin] US Geol Survey, Ft Collins Sci Ctr, Ft Collins, CO 80526 USA. RP Grant, EHC (reprint author), US Geol Survey, Patuxent Wildlife Res Ctr, SO Conte Anadromous Fish Lab, 1 Migratory Way, Turners Falls, MA 01376 USA. EM ehgrant@usgs.gov RI Schmidt, Benedikt/B-8491-2008; Bailey, Larissa/A-2565-2009; Miller, David/E-4492-2012; Waddle, Hardin/D-3845-2009 OI Schmidt, Benedikt/0000-0002-4023-1001; Waddle, Hardin/0000-0003-1940-2133 FU US Geological Survey FX This work was conducted as part of the Amphibian Decline Working Group supported by the John Wesley Powell Center for Analysis and Synthesis, funded by the US Geological Survey. Data deposited at the US Geological Survey's John Wesley Powell Center for Analysis and Synthesis. The authors declare no competing financial interests. This manuscript is contribution #541 of the USGS Amphibian Research and Monitoring Initiative. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. Comments from D. Hocking, P. Toschik and three anonymous reviewers improved the manuscript. NR 44 TC 6 Z9 6 U1 16 U2 45 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD MAY 23 PY 2016 VL 6 AR 25625 DI 10.1038/srep25625 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DM3EQ UT WOS:000376230000001 PM 27212145 ER PT J AU Kasuga, T Bui, M Bernhardt, E Swiecki, T Aram, K Cano, LM Webber, J Brasier, C Press, C Grunwald, NJ Rizzo, DM Garbelotto, M AF Kasuga, Takao Bui, Mai Bernhardt, Elizabeth Swiecki, Tedmund Aram, Kamyar Cano, Liliana M. Webber, Joan Brasier, Clive Press, Caroline Grunwald, Niklaus J. Rizzo, David M. Garbelotto, Matteo TI Host-induced aneuploidy and phenotypic diversification in the Sudden Oak Death pathogen Phytophthora ramorum SO BMC GENOMICS LA English DT Article DE Invasive pathogens; Aneuploidy; Transposable elements; Loss of heterozygosity ID 3 CLONAL LINEAGES; SECONDARY HOMOTHALLISM; RAPID ADAPTATION; PLANT PATHOLOGY; SEQUENCING DATA; FRAGILE SITES; INFESTANS; EVOLUTION; POLYPLOIDY; POPULATION AB Background: Aneuploidy can result in significant phenotypic changes, which can sometimes be selectively advantageous. For example, aneuploidy confers resistance to antifungal drugs in human pathogenic fungi. Aneuploidy has also been observed in invasive fungal and oomycete plant pathogens in the field. Environments conducive to the generation of aneuploids, the underlying genetic mechanisms, and the contribution of aneuploidy to invasiveness are underexplored. We studied phenotypic diversification and associated genome changes in Phytophthora ramorum, a highly destructive oomycete pathogen with a wide host-range that causes Sudden Oak Death in western North America and Sudden Larch Death in the UK. Introduced populations of the pathogen are exclusively clonal. In California, oak (Quercus spp.) isolates obtained from trunk cankers frequently exhibit host-dependent, atypical phenotypes called non-wild type (nwt), apparently without any host-associated population differentiation. Based on a large survey of genotypes from different hosts, we previously hypothesized that the environment in oak cankers may be responsible for the observed phenotypic diversification in P. ramorum. Results: We show that both normal wild type (wt) and nwt phenotypes were obtained when wt P. ramorum isolates from the foliar host California bay (Umbellularia californica) were re-isolated from cankers of artificially-inoculated canyon live oak (Q. chrysolepis). We also found comparable nwt phenotypes in P. ramorum isolates from a bark canker of Lawson cypress (Chamaecyparis lawsoniana) in the UK; previously nwt was not known to occur in this pathogen population. High-throughput sequencing-based analyses identified major genomic alterations including partial aneuploidy and copy-neutral loss of heterozygosity predominantly in nwt isolates. Chromosomal breakpoints were located at or near transposons. Conclusion: This work demonstrates that major genome alterations of a pathogen can be induced by its host species. This is an undocumented type of plant-microbe interaction, and its contribution to pathogen evolution is yet to be investigated, but one of the potential collateral effects of nwt phenotypes may be host survival. C1 [Kasuga, Takao; Bui, Mai] USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. [Bernhardt, Elizabeth; Swiecki, Tedmund] Phytosphere Res, Vacaville, CA 95687 USA. [Aram, Kamyar; Rizzo, David M.] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Cano, Liliana M.] Univ Florida, Dept Plant Pathol, IFAS, Indian River Res & Educ Ctr, Ft Pierce, FL 34945 USA. [Webber, Joan; Brasier, Clive] Forest Res, Farnham GU10 4LH, Surrey, England. [Press, Caroline; Grunwald, Niklaus J.] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. [Garbelotto, Matteo] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA USA. RP Garbelotto, M (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA USA. EM matteog@berkeley.edu RI Cano, Liliana/O-7041-2016; OI Cano, Liliana/0000-0002-7996-4181; Grunwald, Niklaus/0000-0003-1656-7602 FU United States Department of Agriculture - Agriculture Research Service, CRIS Project [5306-22000-014-00D]; United States Department of Agriculture Farm Bill sections [10201, 10007]; United States Department of Agriculture - Animal and Plant Health Inspection Service [12-8130-0191-CA]; United States Department of Agriculture - Forest Service, Sudden Oak Death Management and Monitoring in the Bay Area [10-DG-11052021-214, 11-DG-11052021-111]; Gordon and Betty Moore Foundation; Midpeninsula Regional Open Space District FX We thank R. Jiang and S. Tripathy for sharing gene models and transposable elements in the P. ramorum genome; S. Kamoun for providing support for genome sequencing; J. Yuzon for reading an earlier version of the manuscript; C. Shoemaker and L. Diep for technical assistance; T. Parker for assistance on data analysis; V. Huffman, K. Suslow, K. Kosta, and W. Schweigkofler for supporting inoculation experiments. The authors gratefully acknowledge use of the services and facilities of the National Ornamentals Research Site at Dominican University of California. This project was funded by the United States Department of Agriculture - Agriculture Research Service, CRIS Project # 5306-22000-014-00D to TK; the United States Department of Agriculture Farm Bill sections 10201 and 10007 to TK, DMR, and MG; the United States Department of Agriculture - Animal and Plant Health Inspection Service # 12-8130-0191-CA to TK, DMR, and MG; the United States Department of Agriculture - Forest Service, Sudden Oak Death Management and Monitoring in the Bay Area # 10-DG-11052021-214 and # 11-DG-11052021-111 to EB and TS; the Gordon and Betty Moore Foundation to DMR and MG; and the Midpeninsula Regional Open Space District to EB and TS. NR 81 TC 1 Z9 1 U1 10 U2 17 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD MAY 20 PY 2016 VL 17 AR 385 DI 10.1186/s12864-016-2717-z PG 17 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DO0WL UT WOS:000377499100001 PM 27206972 ER PT J AU Wilkinson, JM Bao, H Ladinig, A Hong, LJ Stothard, P Lunney, JK Plastow, GS Harding, JCS AF Wilkinson, Jamie M. Bao, Hua Ladinig, Andrea Hong, Linjun Stothard, Paul Lunney, Joan K. Plastow, Graham S. Harding, John C. S. TI Genome-wide analysis of the transcriptional response to porcine reproductive and respiratory syndrome virus infection at the maternal/fetal interface and in the fetus SO BMC GENOMICS LA English DT Article DE RNA-sequencing; Transcriptome; Pig; Fetus; PRRSV; Gene ID FETAL IMPLANTATION SITES; GENE-EXPRESSION DATA; IMMUNE-RESPONSES; DENDRITIC CELLS; LYMPH-NODE; DIFFERENTIAL EXPRESSION; NETWORK ANALYSIS; PREGNANT GILTS; UP-REGULATION; VIRAL LOAD AB Background: Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection of pregnant pigs can result in congenital infection and ultimately fetal death. Little is known about immune responses to infection at the maternal-fetal interface and in the fetus itself, or the molecular events behind virus transmission and disease progression in the fetus. To investigate these processes, RNA-sequencing of two sites, uterine endothelium with adherent placental tissue and fetal thymus, was performed 21 days post-challenge on four groups of fetuses selected from a large PRRSV challenge experiment of pregnant gilts: control (CON), uninfected (UNINF), infected (INF), and meconium-stained (MEC) (n = 12/group). Transcriptional analyses consisted of multiple contrasts between groups using two approaches: differential gene expression analysis and weighted gene co-expression network analysis (WGCNA). Biological functions, pathways, and regulators enriched for differentially expressed genes or module members were identified through functional annotation analyses. Expression data were validated by reverse transcription quantitative polymerase chain reaction (RTqPCR) carried out for 16 genes of interest. Results: The immune response to infection in endometrium was mainly adaptive in nature, with the most upregulated genes functioning in either humoral or cell-mediated immunity. In contrast, the expression profile of infected fetal thymus revealed a predominantly innate immune response to infection, featuring the upregulation of genes regulated by type I interferon and pro-inflammatory cytokines. Fetal infection was associated with an increase in viral load coupled with a reduction in T cell signaling in the endometrium that could be due to PRRSV-controlled apoptosis of uninfected bystander cells. There was also evidence for a reduction in TWIST1 activity, a transcription factor involved in placental implantation and maturation, which could facilitate virus transmission or fetal pathology through dysregulation of placental function. Finally, results suggested that events within the fetus could also drive fetal pathology. Thymus samples of meconium-stained fetuses exhibited an increase in the expression of pro-inflammatory cytokine and granulocyte genes previously implicated in swine infectious disease pathology. Conclusions: This study identified major differences in the response to PRRSV infection in the uterine endometrium and fetus at the gene expression level, and provides insight into the molecular basis of virus transmission and disease progression. C1 [Wilkinson, Jamie M.; Bao, Hua; Stothard, Paul; Plastow, Graham S.] Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB, Canada. [Ladinig, Andrea] Univ Vet Med, Univ Clin Swine, Dept Farm Anim & Vet Publ Hlth, Vienna, Austria. [Ladinig, Andrea; Harding, John C. S.] Univ Saskatchewan, Western Coll Vet Med, Dept Large Anim Clin Sci, Saskatoon, SK S7N 0W0, Canada. [Hong, Linjun; Lunney, Joan K.] USDA, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, ARS, Beltsville, MD 20705 USA. [Hong, Linjun] Huazhong Agr Univ, Coll Anim Sci & Technol, Minist Educ, Key Lab Agr Anim Genet Breeding & Reprod, Wuhan, Peoples R China. RP Wilkinson, JM (reprint author), Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB, Canada. EM jamiewilkinson@hotmail.com OI Ladinig, Andrea/0000-0001-5037-7269 FU Genome Canada; Genome Prairie; Saskatchewan Ministry of Agriculture; Alberta Livestock and Meat Agency (ALMA); Alberta Innovates Biosolutions FX The authors wish to thank the numerous scientists and students from the Western College of Veterinary Medicine, Vaccine and Infectious Disease Organization, Prairie Diagnostic Services Inc., and the University of Alberta who assisted with this project. JMW would particularly like to thank those people who agreed to assist with tissue sample collection from the University of Alberta: George Foxcroft, Jenny Patterson, Gina Oliver, Natalie May, Joan Turchinsky, and Hilary Whiting. Carolyn Ashley coordinated the storage and shipping of samples between institutions. Lynn Elmes, Joan Turchinsky, and Craig Wilkinson provided invaluable assistance in establishing a laboratory space and protocols that met the biosafety requirements of this work. Adriano Arantes is thanked for orchestrating the download and storage of the raw sequence data from the McGill Innovation Centre and it's uploading to the NCBI Geo data repository. Tianfu Yang identified genomic regions associated with fetal PRRS susceptibility to which some of the differentially expressed genes were mapped. Predrag Novakovic and Susan Detmer performed histologic evaluations of tissue sections. Jackson Mah and Sam Abrams are thanked for orchestrating the preparation and shipping of samples for RT-qPCR analysis. Funding for the project was generously provided by grants from Genome Canada, Genome Prairie, and the Saskatchewan Ministry of Agriculture. HB and PS are grateful for financial support from the Alberta Livestock and Meat Agency (ALMA) and Alberta Innovates Biosolutions respectively. NR 67 TC 0 Z9 0 U1 2 U2 4 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD MAY 20 PY 2016 VL 17 AR 383 DI 10.1186/s12864-016-2720-4 PG 17 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DO0WK UT WOS:000377499000001 PM 27207143 ER PT J AU Tanprasertsuk, J Li, BX Bernstein, PS Vishwanathan, R Johnson, MA Poon, L Johnson, EJ AF Tanprasertsuk, Jirayu Li, Binxing Bernstein, Paul S. Vishwanathan, Rohini Johnson, Mary Ann Poon, Leonard Johnson, Elizabeth J. TI Relationship between Concentrations of Lutein and StARD3 among Pediatric and Geriatric Human Brain Tissue SO PLOS ONE LA English DT Article ID PIGMENT OPTICAL-DENSITY; MACULAR PIGMENT; CHOLESTEROL TRANSPORT; BINDING-PROTEIN; OLDER-ADULTS; IDENTIFICATION; CAROTENOIDS; ZEAXANTHIN AB Lutein, a dietary carotenoid, selectively accumulates in human retina and brain. While many epidemiological studies show evidence of a relationship between lutein status and cognitive health, lutein's selective uptake in human brain tissue and its potential function in early neural development and cognitive health have been poorly evaluated at a molecular level. The objective of this study was to evaluate the cross-sectional relationship between concentrations of brain lutein and StARD3 (identified as its binding protein in retinal tissue) among three age groups: infants (1-4 months, n = 10), older adults (55-86 years, n = 8), and centenarians (98-105 years, n = 10). Brain lutein concentrations were analyzed by high-performance liquid chromatography and StARD3 levels were analyzed by Western Blot analysis. The strong relationship in infant brains (r = 0.75, P < 0.001) suggests that lutein has a role in neural development. The relationship remained significant but weaker in older adults (r = 0.51, P < 0.05) and insignificant in centenarians (r = 0.08, P > 0.05), seven of whom had mild cognitive impairment (MCI) or dementia. These exploratory findings suggest an age-related decrease or abnormality of StARD3 activity in human brain. Given that StARD3 is also involved in cholesterol transportation, a process that is aberrant in neurodegenerative diseases, the potential protective function of lutein against these diseases remains to be explored. C1 [Tanprasertsuk, Jirayu; Vishwanathan, Rohini; Johnson, Elizabeth J.] Tufts Univ, Jean Mayer USDA Human Nutr, Res Ctr Aging, Boston, MA 02111 USA. [Li, Binxing; Bernstein, Paul S.] Univ Utah, Sch Med, Moran Eye Ctr, Salt Lake City, UT 84132 USA. [Johnson, Mary Ann; Poon, Leonard] Univ Georgia, Inst Gerontol, Athens, GA 30602 USA. RP Johnson, EJ (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr, Res Ctr Aging, Boston, MA 02111 USA. EM elizabeth.johnson@tufts.edu FU USDA [58-1950-4-003]; NIH [1PO1-AG17553, EY-11600, EY-14800]; Abbott Nutrition; DSM Nutritionals; Research to Prevent Blindness FX This work was supported by: USDA agreement 58-1950-4-003 (EJJ), NIH 1PO1-AG17553 (LP), Abbott Nutrition (EJJ), DSM Nutritionals (EJJ), NIH EY-11600 (PSB), NIH EY-14800 (PSB) and an unrestricted grant to the Moran Eye Center of the University of Utah from Research to Prevent Blindness (PSB). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 20 TC 2 Z9 2 U1 2 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 20 PY 2016 VL 11 IS 5 AR e0155488 DI 10.1371/journal.pone.0155488 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DM4BV UT WOS:000376291500016 PM 27205891 ER PT J AU Guo, ZY Gonzalez, JF Hernandez, JN McNeilly, TN Corripio-Miyar, Y Frew, D Morrison, T Yu, P Li, RW AF Guo, Zhengyu Gonzalez, Jorge Francisco Hernandez, Julia N. McNeilly, Tom N. Corripio-Miyar, Yolanda Frew, David Morrison, Tyler Yu, Peng Li, Robert W. TI Possible mechanisms of host resistance to Haemonchus contortus infection in sheep breeds native to the Canary Islands SO SCIENTIFIC REPORTS LA English DT Article ID QUANTITATIVE TRAIT LOCI; GASTROINTESTINAL NEMATODES; DORPER SHEEP; RED MAASAI; AMPHIREGULIN; PARASITES; IDENTIFICATION; INFLAMMATION; HELMINTH; IMMUNITY AB Haemonchus contortus appears to be the most economically important helminth parasite for small ruminant production in many regions of the world. The two sheep breeds native to the Canary Islands display distinctly different resistant phenotypes under both natural and experimental infections. Canaria Hair Breed (CHB) tends to have significantly lower worm burden and delayed and reduced egg production than the susceptible Canaria Sheep (CS). To understand molecular mechanisms underlying host resistance, we compared the abomasal mucosal transcriptome of the two breeds in response to Haemonchus infection using RNAseq technology. The transcript abundance of 711 and 50 genes were significantly impacted by infection in CHB and CS, respectively (false discovery rate <0.05) while 27 of these genes were significantly affected in both breeds. Likewise, 477 and 16 Gene Ontology (GO) terms were significantly enriched in CHB and CS, respectively (P < 1.0 x 10(-4)). A broad range of mechanisms have evolved in resistant CHB to provide protection against the parasite. Our findings suggest that readily inducible acute inflammatory responses, complement activation, accelerated cell proliferation and subsequent tissue repair, and immunity directed against parasite fecundity all contributed to the development of host resistance to parasitic infection in the resistant breed. C1 [Guo, Zhengyu; Yu, Peng] Texas A&M Univ, Dept Elect & Comp Engn, College Stn, TX 77843 USA. [Guo, Zhengyu; Yu, Peng] Texas A&M Univ, TEES AgriLife Ctr Bioinformat & Genom Syst Engn C, College Stn, TX 77843 USA. [Gonzalez, Jorge Francisco; Hernandez, Julia N.] Univ Las Palmas Gran Canaria, Dept Anim Pathol, Fac Vet Med, Las Palmas Gran Canaria, Spain. [McNeilly, Tom N.; Corripio-Miyar, Yolanda; Frew, David; Morrison, Tyler] Moredun Res Inst, Edinburgh EH26 0PZ, Midlothian, Scotland. [Li, Robert W.] USDA ARS, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. RP Yu, P (reprint author), Texas A&M Univ, Dept Elect & Comp Engn, College Stn, TX 77843 USA.; Yu, P (reprint author), Texas A&M Univ, TEES AgriLife Ctr Bioinformat & Genom Syst Engn C, College Stn, TX 77843 USA.; Gonzalez, JF (reprint author), Univ Las Palmas Gran Canaria, Dept Anim Pathol, Fac Vet Med, Las Palmas Gran Canaria, Spain.; Li, RW (reprint author), USDA ARS, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. EM jorgefrancisco.gonzalez@ulpgc.es; pengyu.bio@gmail.com; Robert.Li@ars.usda.gov OI Corripio-Miyar, Yolanda/0000-0002-1344-7405 FU Spanish National grant [AGL2009/09985]; Fondo Social Europeo (FSE); TEES-AgriLife Center for Bioinformatics and Genomic Systems Engineering (CBGSE) at Texas AM University; ECE department and Texas A&M Engineering Experiment Station/Dwight Look College of Engineering at Texas AM University; Agencia Canaria de Investigacion, Innovacion y Sociedad de la Informacion; Scottish Government; European Union's Horizon 2020 research and innovation programme [635408] FX Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture (USDA). The USDA is an equal opportunity provider and employer. This trial was partially supported by Spanish National grant (AGL2009/09985) and Fondo Social Europeo (FSE). ZG was supported by funding from TEES-AgriLife Center for Bioinformatics and Genomic Systems Engineering (CBGSE) at Texas A&M University. PY was supported by startup funding from the ECE department and Texas A&M Engineering Experiment Station/Dwight Look College of Engineering at Texas A&M University. JNH was sponsored by Agencia Canaria de Investigacion, Innovacion y Sociedad de la Informacion and Fondo Social Europeo (FSE). TNM and DF received funding from the Scottish Government. YCM is supported by European Union's Horizon 2020 research and innovation programme under grant agreement No. 635408. NR 40 TC 2 Z9 2 U1 0 U2 1 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD MAY 20 PY 2016 VL 6 AR 26200 DI 10.1038/srep26200 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DM7QK UT WOS:000376554900001 PM 27197554 ER PT J AU Malapi-Wight, M Demers, JE Veltri, D Marra, RE Crouch, JA AF Malapi-Wight, Martha Demers, Jill E. Veltri, Daniel Marra, Robert E. Crouch, Jo Anne TI LAMP Detection Assays for Boxwood Blight Pathogens: A Comparative Genomics Approach SO SCIENTIFIC REPORTS LA English DT Article ID MEDIATED ISOTHERMAL AMPLIFICATION; CALONECTRIA-PSEUDONAVICULATA; CYLINDROCLADIUM-BUXICOLA; VIRUS DIAGNOSTICS; VISUAL DETECTION; PLANT-PATHOGENS; STEM BLIGHT; BOX BLIGHT; 1ST REPORT; DNA AB Rapid and accurate molecular diagnostic tools are critical to efforts to minimize the impact and spread of emergent pathogens. The identification of diagnostic markers for novel pathogens presents several challenges, especially in the absence of information about population diversity and where genetic resources are limited. The objective of this study was to use comparative genomics datasets to find unique target regions suitable for the diagnosis of two fungal species causing a newly emergent blight disease of boxwood. Candidate marker regions for loop-mediated isothermal amplification (LAMP) assays were identified from draft genomes of Calonectria henricotiae and C. pseudonaviculata, as well as three related species not associated with this disease. To increase the probability of identifying unique targets, we used three approaches to mine genome datasets, based on (i) unique regions, (ii) polymorphisms, and (iii) presence/absence of regions across datasets. From a pool of candidate markers, we demonstrate LAMP assay specificity by testing related fungal species, common boxwood pathogens, and environmental samples containing 445 diverse fungal taxa. This comparative-genomics-based approach to the development of LAMP diagnostic assays is the first of its kind for fungi and could be easily applied to diagnostic marker development for other newly emergent plant pathogens. C1 [Malapi-Wight, Martha; Demers, Jill E.; Veltri, Daniel; Crouch, Jo Anne] ARS, USDA, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Veltri, Daniel] ARS, Oak Ridge Inst Sci & Educ, Res Participat Program, Oak Ridge, TN 37831 USA. [Marra, Robert E.] Connecticut Agr Expt Stn, Dept Plant Pathol & Ecol, New Haven, CT 06504 USA. [Malapi-Wight, Martha] Anim & Plant Hlth Inspect Serv, USDA, Plant Germplasm Quarantine Program, Beltsville, MD 20705 USA. RP Crouch, JA (reprint author), ARS, USDA, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. EM joanne.crouch@ars.usda.gov OI Crouch, Jo Anne/0000-0001-6886-8090; Veltri, Daniel/0000-0002-6101-6693; Malapi-Wight, Martha/0000-0003-2249-6257 FU USDA-APHIS Farm Bill [10201, 10004]; USDA-ARS Floricultural and Nursery Research Fund; Class of USDA-ARS Headquarters Research Associate Award; DOE [DE-AC05-06OR23100] FX This work was supported by funds from the USDA-APHIS 2012 Farm Bill 10201 to JAC and REM, and funds from the USDA-ARS Floricultural and Nursery Research Fund to JAC. MMW is supported by a Class of 2013 USDA-ARS Headquarters Research Associate Award to JAC; JED is supported by a Class of 2012 USDA-ARS Headquarters Research Associate Award to Lisa Castlebury; and DV is supported through 2013 and 2014 USDA-APHIS Farm Bill 10201 and 10004 grants to JAC. We thank Lisa Castlebury for helpful discussion of this manuscript; Kurt Heungens and Bjorn Gehesquiere for sharing fungal isolates; and Richard Buckley for the landscape images. This research was supported in part by an appointment to the Agricultural Research Service (ARS) Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and the U.S. Department of Agriculture (USDA). ORISE is managed by ORAU under DOE contract number DE-AC05-06OR23100. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer. NR 47 TC 1 Z9 1 U1 4 U2 10 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD MAY 20 PY 2016 VL 6 AR 26140 DI 10.1038/srep26140 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DM7QC UT WOS:000376554100001 PM 27199028 ER PT J AU Carter, JM Lin, A Clotilde, L Lesho, M AF Carter, John M. Lin, Andrew Clotilde, Laurie Lesho, Matthew TI Rapid, Multiplexed Characterization of Shiga Toxin-Producing Escherichia coil (STEC) Isolates Using Suspension Array Technology SO FRONTIERS IN MICROBIOLOGY LA English DT Review DE E. coil; Shiga toxin; immunoassay; PCR; microbead ID MICROBEAD-BASED IMMUNOASSAY; COLI INFECTIONS; UNITED-STATES; IDENTIFICATION; PATHOGENS; O157; VIRULENCE; SAMPLES; CATTLE AB Molecular methods have emerged as the most reliable techniques to detect and characterize pathogenic Escherichia coli. These molecular techniques include conventional single analyte and multiplex PCR, PCR followed by microarray detection, pulsed-field gel electrophoresis (PFGE), and whole genome sequencing. The choice of methods used depends upon the specific needs of the particular study. One versatile method involves detecting serogroup-specific markers by hybridization or binding to encoded microbeads in a suspension array. This molecular serotyping method has been developed and adopted for investigating E. coli outbreaks. The major advantages of this technique are the ability to simultaneously serotype E. coli and detect the presence of virulence and pathogenicity markers. Here, we describe the development of a family of multiplex molecular serotyping methods for Shiga toxin-producing E. colt, compare their performance to traditional serotyping methods, and discuss the cost-benefit balance of these methods in the context of various food safety objectives. C1 [Carter, John M.] ARS, Pacific West Area, Western Reg Res Ctr, Produce Safety & Microbiol Res,USDA, Albany, CA USA. [Lin, Andrew] US FDA, ORA PA FO SAN LB, Off Global Regulatory Operat & Policy, Oceans Reefs & Aquariums,US Dept Hlth & Human Ser, Alameda, CA USA. [Clotilde, Laurie] MagArray Inc, Milpitas, CA USA. [Lesho, Matthew] Luminex Corp, Austin, TX USA. RP Carter, JM (reprint author), ARS, Pacific West Area, Western Reg Res Ctr, Produce Safety & Microbiol Res,USDA, Albany, CA USA. EM mark@safetraces.com FU U.S. Department of Agriculture [108, CRIS 5325-42000-043-00D]; FDA San Francisco Laboratory; FDA Office of Regulatory Science; CFSAN Office of Regulatory Science FX U.S. Department of Agriculture work was funded under National Program 108, Food Safety, CRIS 5325-42000-043-00D. The authors would also like to thank the FDA San Francisco Laboratory, FDA Office of Regulatory Science, and CFSAN Office of Regulatory Science for their support of this research. NR 35 TC 0 Z9 0 U1 7 U2 9 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD MAY 20 PY 2016 VL 7 AR 439 DI 10.3389/fmicb.2016.00439 PG 6 WC Microbiology SC Microbiology GA DM2AU UT WOS:000376149000001 PM 27242670 ER PT J AU Fry, LM Schneider, DA Frevert, CW Nelson, DD Morrison, WI Knowles, DP AF Fry, Lindsay M. Schneider, David A. Frevert, Charles W. Nelson, Danielle D. Morrison, W. Ivan Knowles, Donald P. TI East Coast Fever Caused by Theileria parva Is Characterized by Macrophage Activation Associated with Vasculitis and Respiratory Failure SO PLOS ONE LA English DT Article ID SCAVENGER RECEPTOR CD163; TOXOPLASMA-GONDII; IMMUNOHISTOCHEMICAL CHARACTERIZATION; PARASITIC INFECTIONS; SOLUBLE CD163; CARRIER STATE; NITRIC-OXIDE; IN-VITRO; CATTLE; LYMPHOCYTES AB Respiratory failure and death in East Coast Fever (ECF), a clinical syndrome of African cattle caused by the apicomplexan parasite Theileria parva, has historically been attributed to pulmonary infiltration by infected lymphocytes. However, immunohistochemical staining of tissue from T. parva infected cattle revealed large numbers of CD3- and CD20-negative intralesional mononuclear cells. Due to this finding, we hypothesized that macrophages play an important role in Theileria parva disease pathogenesis. Data presented here demonstrates that terminal ECF in both Holstein and Boran cattle is largely due to multisystemic histiocytic responses and resultant tissue damage. Furthermore, the combination of these histologic changes with the clinical findings, including lymphadenopathy, prolonged pyrexia, multi-lineage leukopenia, and thrombocytopenia is consistent with macrophage activation syndrome. All animals that succumbed to infection exhibited lymphohistiocytic vasculitis of small to medium caliber blood and lymphatic vessels. In pulmonary, lymphoid, splenic and hepatic tissues from Holstein cattle, the majority of intralesional macrophages were positive for CD163, and often expressed large amounts of IL-17. These data define a terminal ECF pathogenesis in which parasite-driven lymphoproliferation leads to secondary systemic macrophage activation syndrome, mononuclear vasculitis, pulmonary edema, respiratory failure and death. The accompanying macrophage phenotype defined by CD163 and IL-17 is presented in the context of this pathogenesis. C1 [Fry, Lindsay M.; Schneider, David A.; Knowles, Donald P.] USDA, Anim Dis Res Unit, Agr Res Serv, Pullman, WA USA. [Fry, Lindsay M.; Schneider, David A.; Nelson, Danielle D.; Knowles, Donald P.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Morrison, W. Ivan] Univ Edinburgh, Roslin Inst, Easter Bush, Midlothian, Scotland. [Frevert, Charles W.] Univ Washington, Sch Med, Dept Comparat Med, Ctr Lung Biol, Seattle, WA 98195 USA. RP Fry, LM (reprint author), USDA, Anim Dis Res Unit, Agr Res Serv, Pullman, WA USA.; Fry, LM (reprint author), Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. EM lfry@vetmed.wsu.edu FU USAID [AID-BFS-P-13-00002]; Bill and Melinda Gates Foundation [OPP1078791] FX This work was supported by USAID AID-BFS-P-13-00002 and the Bill and Melinda Gates Foundation grant OPP1078791. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 60 TC 2 Z9 2 U1 3 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 19 PY 2016 VL 11 IS 5 AR e0156004 DI 10.1371/journal.pone.0156004 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DM4BR UT WOS:000376291100148 PM 27195791 ER PT J AU Liu, JJ Chan, D Xiang, Y Williams, H Li, XR Sniezko, RA Sturrock, RN AF Liu, Jun-Jun Chan, Danelle Xiang, Yu Williams, Holly Li, Xiao-Rui Sniezko, Richard A. Sturrock, Rona N. TI Characterization of Five Novel Mitoviruses in the White Pine Blister Rust Fungus Cronartium ribicola SO PLOS ONE LA English DT Article ID OPHIOSTOMA-NOVO-ULMI; DOUBLE-STRANDED-RNA; CHESTNUT BLIGHT FUNGUS; SCLEROTINIA-SCLEROTIORUM; HYPOVIRULENT STRAIN; BOTRYTIS-CINEREA; PLANT; VIRUSES; ISOLATE; SEQUENCE AB The white pine blister rust (WPBR) fungus Cronartium ribicola (J.C. Fisch.) is an exotic invasive forest pathogen causing severe stem canker disease of native white pine trees (subgenus Strobus) in North America. The present study reports discovery of five novel mitoviruses in C. ribicola by deep RNA sequencing. The complete genome of each mitovirus was determined by rapid amplification of cDNA ends (RACE) and reverse transcriptase-polymerase chain reaction (RT-PCR). A single open reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRp) was detected in each of the viral genomes using mitochondrial genetic codes. Phylogenetic analysis indicated that the C. ribicola mitoviruses (CrMV1 to CrMV5) are new putative species of the genus Mitovirus. qRT-PCR and RNA-Seq analyses revealed that viral RNAs were significantly increased in fungal mycelia in cankered pine stems compared to expression during two different stages of spore development, suggesting that viral genome replication and transcription benefit from active growth of the host fungus. CrMVs were widespread with relatively high levels of minor allele frequency (MAF) in western North America. As the first report of mitoviruses in the Class Pucciniomycetes, this work allows further investigation of the dynamics of a viral community in the WPBR pathosystem, including potential impacts that may affect pathogenicity and virulence of the host fungus. C1 [Liu, Jun-Jun; Chan, Danelle; Williams, Holly; Li, Xiao-Rui; Sturrock, Rona N.] Nat Resources Canada, Pacific Forestry Ctr, Canadian Forest Serv, Victoria, BC V8Z 1M5, Canada. [Xiang, Yu] Agr & Agri Food Canada, Pacific Agrifood Res Ctr, Summerland, BC V0H 1Z0, Canada. [Sniezko, Richard A.] US Forest Serv, USDA, Dorena Genet Resource Ctr, Cottage Grove, OR 97424 USA. RP Liu, JJ (reprint author), Nat Resources Canada, Pacific Forestry Ctr, Canadian Forest Serv, Victoria, BC V8Z 1M5, Canada. EM jun-jun.liu@canada.ca FU PFC-CFS DG's fund; CFS-A base program; CFS-GRDI grant; US-FS FX J-JL and RNS received financial support from PFC-CFS DG's fund, CFS-A base program, and the CFS-GRDI grant. RAS received grant from US-FS that allowed continuous collaboration between CFS and US-FS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 50 TC 0 Z9 0 U1 7 U2 10 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 19 PY 2016 VL 11 IS 5 AR e0154267 DI 10.1371/journal.pone.0154267 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DM4BR UT WOS:000376291100011 PM 27196406 ER PT J AU Stevens, JT Safford, HD North, MP Fried, JS Gray, AN Brown, PM Dolanc, CR Dobrowski, SZ Falk, DA Farris, CA Franklin, JF Fule, PZ Hagmann, RK Knapp, EE Miller, JD Smith, DF Swetnam, TW Taylor, AH AF Stevens, Jens T. Safford, Hugh D. North, Malcolm P. Fried, Jeremy S. Gray, Andrew N. Brown, Peter M. Dolanc, Christopher R. Dobrowski, Solomon Z. Falk, Donald A. Farris, Calvin A. Franklin, Jerry F. Fule, Peter Z. Hagmann, R. Keala Knapp, Eric E. Miller, Jay D. Smith, Douglas F. Swetnam, Thomas W. Taylor, Alan H. TI Average Stand Age from Forest Inventory Plots Does Not Describe Historical Fire Regimes in Ponderosa Pine and Mixed-Conifer Forests of Western North America SO PLOS ONE LA English DT Article ID YOSEMITE-NATIONAL-PARK; LAKE TAHOE BASIN; KLAMATH MOUNTAINS; TREE RECRUITMENT; SPATIAL-PATTERNS; CASCADE RANGE; UNITED-STATES; SOUTHERN CASCADES; ROCKY-MOUNTAINS; BOREAL FOREST AB Quantifying historical fire regimes provides important information for managing contemporary forests. Historical fire frequency and severity can be estimated using several methods; each method has strengths and weaknesses and presents challenges for interpretation and verification. Recent efforts to quantify the timing of historical high-severity fire events in forests of western North America have assumed that the "stand age" variable from the US Forest Service Forest Inventory and Analysis (FIA) program reflects the timing of historical high-severity (i.e. stand-replacing) fire in ponderosa pine and mixed-conifer forests. To test this assumption, we re-analyze the dataset used in a previous analysis, and compare information from fire history records with information from co-located FIA plots. We demonstrate that 1) the FIA stand age variable does not reflect the large range of individual tree ages in the FIA plots: older trees comprised more than 10% of pre-stand age basal area in 58% of plots analyzed and more than 30% of pre-stand age basal area in 32% of plots, and 2) recruitment events are not necessarily related to high-severity fire occurrence. Because the FIA stand age variable is estimated from a sample of tree ages within the tree size class containing a plurality of canopy trees in the plot, it does not necessarily include the oldest trees, especially in uneven-aged stands. Thus, the FIA stand age variable does not indicate whether the trees in the predominant size class established in response to severe fire, or established during the absence of fire. FIA stand age was not designed to measure the time since a stand-replacing disturbance. Quantification of historical "mixed-severity" fire regimes must be explicit about the spatial scale of high-severity fire effects, which is not possible using FIA stand age data. C1 [Stevens, Jens T.; North, Malcolm P.] Univ Calif Davis, John Muir Inst Environm, Davis, CA 95616 USA. [Safford, Hugh D.] US Forest Serv, USDA, Pacific Southwest Reg, Vallejo, CA 94592 USA. [Safford, Hugh D.] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. [North, Malcolm P.] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95616 USA. [Fried, Jeremy S.] US Forest Serv, USDA, Forest Inventory & Anal Program, Pacific Northwest Res Stn, Portland, OR 97205 USA. [Gray, Andrew N.] US Forest Serv, USDA, Forest Inventory & Anal Program, Pacific Northwest Res Stn, Corvallis, OR 97331 USA. [Brown, Peter M.] Rocky Mt Tree Ring Res, Ft Collins, CO 80526 USA. [Dolanc, Christopher R.] Mercyhurst Univ, Dept Biol, Erie, PA 16546 USA. [Dobrowski, Solomon Z.] Univ Montana, Dept Forest Management, Missoula, MT 59812 USA. [Falk, Donald A.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ 85721 USA. [Falk, Donald A.; Swetnam, Thomas W.] Univ Arizona, Tree Ring Res Lab, Tucson, AZ 85721 USA. [Farris, Calvin A.] Natl Pk Serv, Pacific West Reg Fire & Aviat Management, Klamath Falls, OR 97601 USA. [Franklin, Jerry F.; Hagmann, R. Keala] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Fule, Peter Z.] No Arizona Univ, Sch Forestry, Flagstaff, AZ 86011 USA. [Knapp, Eric E.] US Forest Serv, USDA, Pacific Southwest Res Stn, Redding, CA 96002 USA. [Miller, Jay D.] US Forest Serv, USDA, Pacific Southwest Reg Fire & Aviat Management, McClellan, CA 95652 USA. [Smith, Douglas F.] Yosemite Natl Pk, Yosemite, CA 95389 USA. Penn State Univ, Dept Geog, University Pk, PA 16802 USA. [Taylor, Alan H.] Penn State Univ, Earth & Environm Syst Inst, University Pk, PA 16802 USA. RP Stevens, JT (reprint author), Univ Calif Davis, John Muir Inst Environm, Davis, CA 95616 USA. EM jtstevens@ucdavis.edu OI Stevens, Jens/0000-0002-2234-1960 FU John Muir Institute of the Environment at the University of California, Davis FX We thank David Orwig, Dennis Odion and Chad Hanson, along with two anonymous reviewers, for helpful comments on this manuscript. Alexis Arizpe provided age structure and fire history data for the Jemez Mountains. Support for JTS provided by the John Muir Institute of the Environment at the University of California, Davis. NR 107 TC 1 Z9 1 U1 6 U2 12 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 19 PY 2016 VL 11 IS 5 AR e0147688 DI 10.1371/journal.pone.0147688 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DM4BR UT WOS:000376291100001 PM 27196621 ER PT J AU Shih, HY Sciume, G Mikami, Y Guo, LY Sun, HW Brooks, SR Urban, JF Davis, FP Kanno, Y O'Shea, JJ AF Shih, Han-Yu Sciume, Giuseppe Mikami, Yohei Guo, Liying Sun, Hong-Wei Brooks, Stephen R. Urban, Joseph F., Jr. Davis, Fred P. Kanno, Yuka O'Shea, John J. TI Developmental Acquisition of Regulomes Underlies Innate Lymphoid Cell Functionality SO CELL LA English DT Article ID IMMUNOLOGICAL GENOME PROJECT; MURINE BONE-MARROW; TRANSCRIPTION FACTOR; NATURAL-KILLER; T-BET; ADAPTIVE IMMUNITY; ENHANCERS; FATE; IDENTIFICATION; MACROPHAGE AB Innate lymphoid cells (ILCs) play key roles in host defense, barrier integrity, and homeostasis and mirror adaptive CD4(+) T helper (Th) cell subtypes in both usage of effector molecules and transcription factors. To better understand the relationship between ILC subsets and their Th cell counterparts, we measured genome-wide chromatin accessibility. We find that chromatin in proximity to effector genes is selectively accessible in ILCs prior to high-level transcription upon activation. Accessibility of these regions is acquired in a stepwise manner during development and changes little after in vitro or in vivo activation. Conversely, dramatic chromatin remodeling occurs in naive CD4(+) T cells during Th cell differentiation using a type-2-infection model. This alteration results in a substantial convergence of Th2 cells toward ILC2 regulomes. Our data indicate extensive sharing of regulatory circuitry across the innate and adaptive compartments of the immune system, in spite of their divergent developing pathways. C1 [Shih, Han-Yu; Sciume, Giuseppe; Mikami, Yohei; Davis, Fred P.; Kanno, Yuka; O'Shea, John J.] NIAMSD, Lymphocyte Cell Biol Sect, Mol Immunol & Inflammat Branch, NIH, Bethesda, MD 20892 USA. [Guo, Liying] NIAID, Immunol Lab, NIH, Bldg 10, Bethesda, MD 20892 USA. [Sun, Hong-Wei; Brooks, Stephen R.] NIAMSD, Biodata Min & Discovery Sect, NIH, Bethesda, MD 20892 USA. [Urban, Joseph F., Jr.] ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Shih, HY; O'Shea, JJ (reprint author), NIAMSD, Lymphocyte Cell Biol Sect, Mol Immunol & Inflammat Branch, NIH, Bethesda, MD 20892 USA. EM han-yu.shih@nih.gov; john.oshea@nih.gov RI Sciume, Giuseppe/K-8985-2016 OI Sciume, Giuseppe/0000-0003-0131-512X FU NIAMS FX We thank Drs. Y. Belkaid, K. Zhao, V. Sartorelli, J. Zhu, I. Fraser, and A. Poholek for critically reading this manuscript. We also thank G. Gutierrez-Cruz (Genome Analysis Core Facility, NIAMS) and J. Simone, J. Lay, and K. Tinsley (Flow Cytometry Section, NIAMS) for their technical support. This study utilized the high-performance computational capabilities of the Biowulf Linux cluster at the NIH. This work was supported by the Intramural Research Programs of the NIAMS. NR 62 TC 12 Z9 12 U1 2 U2 7 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 0092-8674 EI 1097-4172 J9 CELL JI Cell PD MAY 19 PY 2016 VL 165 IS 5 BP 1120 EP 1133 DI 10.1016/j.cell.2016.04.029 PG 14 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA DM6QT UT WOS:000376478200013 PM 27156451 ER PT J AU Wang, XY Wei, K Yang, ZQ Jennings, DE Duan, JJ AF Wang, Xiaoyi Wei, Ke Yang, Zhongqi Jennings, David E. Duan, Jian J. TI Effects of biotic and abiotic factors on phenotypic partitioning of wing morphology and development in Sclerodermus pupariae (Hymenoptera: Bethylidae) SO SCIENTIFIC REPORTS LA English DT Article ID ECTOPARASITOID MELITTOBIA-DIGITATA; AGRILUS-PLANIPENNIS COLEOPTERA; DIFFERENTIAL GENE-EXPRESSION; LOCAL MATE COMPETITION; EMERALD ASH BORER; LIFE-HISTORY; SEX-RATIOS; ORTHOPTERA-GRYLLIDAE; FLIGHT CAPABILITY; CLUTCH SIZE AB Wing phenotype polymorphism is commonly observed in insects, yet little is known about the influence of environmental cues on the development or expression of the alternative phenotypes. Here, we report how both biotic and abiotic factors affect the wing morph differentiation of a bethylid parasitoid Sclerodermus pupariae. The percentage of winged female parasitoid progeny increased exponentially with temperature between 20 degrees C to 30 degrees C. Low intensity light and short-day photoperiod conditions also significantly induced the development of winged morphs. Interestingly, wingless maternal parasitoids produced more winged progeny. Furthermore, the degree of wing dimorphism was significantly influenced by the interactions between light intensity and maternal wing morphs. The percentage of winged female progeny was not significantly influenced by foundress densities, but increased significantly with parasitoid brood sizes. However, the percentage of male progeny increased significantly with the densities of maternal parasitoids. Our findings highlight the phenotypic partitioning of wing morphology and development in the parasitoid S. pupariae under varied environmental cues, and reveal the most favourable conditions for the production of winged females in this bethylid wasp. It is thus possible to increase winged female parasitoid production for the purposes of biological control by manipulation of biotic and abiotic conditions. C1 [Wang, Xiaoyi; Wei, Ke; Yang, Zhongqi] Chinese Acad Forestry, Res Inst Forest Ecol, State Forestry Adm, Key Lab Forest Protect, 2 Dongxiaofu,Xiangshan Rd, Beijing 100091, Peoples R China. [Jennings, David E.] Univ Maryland, Dept Entomol, 4112 Plant Sci Bldg, College Pk, MD 20742 USA. [Duan, Jian J.] ARS, USDA, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. RP Wang, XY (reprint author), Chinese Acad Forestry, Res Inst Forest Ecol, State Forestry Adm, Key Lab Forest Protect, 2 Dongxiaofu,Xiangshan Rd, Beijing 100091, Peoples R China. EM xywang@caf.ac.cn OI Jennings, David/0000-0001-7179-3069 FU National Natural Science Foundation of China [31370654] FX All experiments were carried out in the laboratory of the Beneficial Insects Introduction Research Unit, USDA-ARS, Newark, DE 19713, USA. We are grateful to Jonathan Schmude, Kristi Larson, and Jackie Hoban for rearing of emerald ash borers, and two anonymous reviewers whose comments greatly improved the manuscript. This work was funded by the National Natural Science Foundation of China (31370654). NR 67 TC 1 Z9 1 U1 7 U2 18 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD MAY 19 PY 2016 VL 6 AR 26408 DI 10.1038/srep26408 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DM0WX UT WOS:000376067700001 PM 27194095 ER PT J AU Craven, D Isbell, F Manning, P Connolly, J Bruelheide, H Ebeling, A Roscher, C van Ruijven, J Weigelt, A Wilsey, B Beierkuhnlein, C de Luca, E Griffin, JN Hautier, Y Hector, A Jentsch, A Kreyling, J Lanta, V Loreau, M Meyer, ST Mori, AS Naeem, S Palmborg, C Polley, HW Reich, PB Schmid, B Siebenkas, A Seabloom, E Thakur, MP Tilman, D Vogel, A Eisenhauer, N AF Craven, Dylan Isbell, Forest Manning, Pete Connolly, John Bruelheide, Helge Ebeling, Anne Roscher, Christiane van Ruijven, Jasper Weigelt, Alexandra Wilsey, Brian Beierkuhnlein, Carl de Luca, Enrica Griffin, John N. Hautier, Yann Hector, Andy Jentsch, Anke Kreyling, Juergen Lanta, Vojtech Loreau, Michel Meyer, Sebastian T. Mori, Akira S. Naeem, Shahid Palmborg, Cecilia Polley, H. Wayne Reich, Peter B. Schmid, Bernhard Siebenkas, Alrun Seabloom, Eric Thakur, Madhav P. Tilman, David Vogel, Anja Eisenhauer, Nico TI Plant diversity effects on grassland productivity are robust to both nutrient enrichment and drought SO PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Article DE plant diversity; global change drivers; resource amendment; resource reduction; soil nutrients; drought ID GLOBAL ENVIRONMENTAL-CHANGE; BIODIVERSITY LOSS; ECOSYSTEM PRODUCTIVITY; FUNCTIONAL TRAITS; SPECIES RICHNESS; STABILITY; MECHANISMS; COMPLEMENTARITY; RESPONSES; CARBON AB Global change drivers are rapidly altering resource availability and biodiversity. While there is consensus that greater biodiversity increases the functioning of ecosystems, the extent to which biodiversity buffers ecosystem productivity in response to changes in resource availability remains unclear. We use data from 16 grassland experiments across North America and Europe that manipulated plant species richness and one of two essential resources-soil nutrients or water-to assess the direction and strength of the interaction between plant diversity and resource alteration on above-ground productivity and net biodiversity, complementarity, and selection effects. Despite strong increases in productivity with nutrient addition and decreases in productivity with drought, we found that resource alterations did not alter biodiversity-ecosystem functioning relationships. Our results suggest that these relationships are largely determined by increases in cornplementarity effects along plant species richness gradients. Although nutrient addition reduced complementarity effects at high diversity, this appears to be due to high biomass in monocultures under nutrient enrichment. Our results indicate that diversity and the complementarity of species are important regulators of grassland ecosystem productivity, regardless of changes in other drivers of ecosystem function. C1 [Craven, Dylan; Bruelheide, Helge; Roscher, Christiane; Weigelt, Alexandra; Thakur, Madhav P.; Vogel, Anja; Eisenhauer, Nico] German Ctr Integrat Biodivers Res iDiv, Deutsch Pl 5e, D-04103 Leipzig, Germany. [Craven, Dylan; Weigelt, Alexandra; Thakur, Madhav P.; Eisenhauer, Nico] Univ Leipzig, Inst Biol, Johannisallee 21, D-04103 Leipzig, Germany. [Isbell, Forest; Seabloom, Eric; Tilman, David] Univ Minnesota Twin Cities, Dept Ecol Evolut & Behav, St Paul, MN 55108 USA. [Manning, Pete] Univ Bern, Inst Plant Sci, CH-3013 Bern, Switzerland. [Manning, Pete] Biodivers & Climate Res Ctr, Senckenberganlage 25, D-60325 Frankfurt, Germany. [Connolly, John] Univ Coll Dublin, Sch Math & Stat, Ecol & Environm Modelling Grp, Dublin 4, Ireland. [Bruelheide, Helge] Univ Halle Wittenberg, Inst Biol Geobot & Bot Garden, D-06108 Halle, Germany. [Ebeling, Anne] Univ Jena, Inst Ecol, Dornburger Str 159, D-07743 Jena, Germany. [Roscher, Christiane] Helmholtz Ctr Environm Res, Dept Physiol Divers, Leipzig, Germany. [van Ruijven, Jasper] Wageningen Univ, Plant Ecol & Nat Conservat Grp, POB 47, NL-6700 AA Wageningen, Netherlands. [Wilsey, Brian] Iowa State Univ, Dept Ecol Evolut & Organismal Biol, Ames, IA 50011 USA. [Beierkuhnlein, Carl] Univ Bayreuth, Dept Biogeog, BayCEER, D-95440 Bayreuth, Germany. [de Luca, Enrica; Schmid, Bernhard] Univ Zurich, Inst Evolutionary Biol & Environm Studies, CH-8057 Zurich, Switzerland. [Griffin, John N.] Swansea Univ, Coll Sci, Dept Biosci, Swansea, W Glam, Wales. [Hautier, Yann] Univ Utrecht, Dept Biol, Ecol & Biodivers Grp, Padualaan 8, NL-3584 Utrecht, Netherlands. [Hector, Andy] Univ Oxford, Dept Plant Sci, S Parks Rd, Oxford OX1 3RB, England. [Jentsch, Anke] Univ Bayreuth, Dept Disturbance Ecol, BayCEER, D-95440 Bayreuth, Germany. [Kreyling, Juergen] Ernst Moritz Arndt Univ Greifswald, Inst Bot & Landscape Ecol, D-17487 Greifswald, Germany. [Lanta, Vojtech] Univ South Bohemia, Dept Bot, Fac Sci, Branisovska 31, Ceske Budejovice 37005, Czech Republic. [Loreau, Michel] CNRS, Theoret & Expt Ecol Stn, Ctr Biodivers Theory & Modelling, F-09200 Moulis, France. [Loreau, Michel] Univ Toulouse 3, F-09200 Moulis, France. [Meyer, Sebastian T.; Vogel, Anja] Tech Univ Munich, Sch Life Sci, Dept Ecol & Ecosyst Management, D-85354 Freising Weihenstephan, Germany. [Mori, Akira S.] Yokohama Natl Univ, Grad Sch Environm & Informat Sci, 79-7 Tokiwadai, Yokohama, Kanagawa 2408501, Japan. [Naeem, Shahid] Columbia Univ, Dept Ecol Evolut & Environm Biol, New York, NY 10027 USA. [Palmborg, Cecilia] Swedish Univ Agr Sci, Dept Agr Res Northern Sweden, S-90183 Umea, Sweden. [Polley, H. Wayne] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Reich, Peter B.] Univ Minnesota, Dept Forest Resources, 1530 North Cleveland Ave, St Paul, MN 55108 USA. [Reich, Peter B.] Univ Western Sydney, Hawkesbury Inst Environm, Penrith, NSW 2751, Australia. [Siebenkas, Alrun] UFZ Helmholtz Ctr Environm Res, Dept Community Ecol, Theodor Lieser Str 4, D-06120 Halle, Germany. RP Craven, D (reprint author), German Ctr Integrat Biodivers Res iDiv, Deutsch Pl 5e, D-04103 Leipzig, Germany.; Craven, D (reprint author), Univ Leipzig, Inst Biol, Johannisallee 21, D-04103 Leipzig, Germany. EM dylan.craven@aya.yale.edu RI Manning, Peter/I-6523-2012; Hautier, Yann/D-5426-2015; OI Manning, Peter/0000-0002-7940-2023; Hautier, Yann/0000-0003-4347-7741; Isbell, Forest/0000-0001-9689-769X; Siebenkas, Alrun/0000-0001-9676-7094; Eisenhauer, Nico/0000-0002-0371-6720; Craven, Dylan/0000-0003-3940-833X FU sDiv, the Synthesis Centre of the German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig (DFG) [FZT 118]; TULIP Laboratory of Excellence [ANR-10-LABX-41] FX This paper is a joint effort of the STABILITY group funded by sDiv (www.idiv.de/stability), the Synthesis Centre of the German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig (DFG FZT 118). M.L. was supported by the TULIP Laboratory of Excellence (ANR-10-LABX-41). NR 60 TC 5 Z9 5 U1 47 U2 82 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8436 EI 1471-2970 J9 PHILOS T R SOC B JI Philos. Trans. R. Soc. B-Biol. Sci. PD MAY 19 PY 2016 VL 371 IS 1694 AR 20150277 DI 10.1098/rstb.2015.0277 PG 8 WC Biology SC Life Sciences & Biomedicine - Other Topics GA DL8MQ UT WOS:000375896500012 ER PT J AU Flores-Moreno, H Reich, PB Lind, EM Sullivan, LL Seabloom, EW Yahdjian, L MacDougall, AS Reichmann, LG Alberti, J Baez, S Bakker, JD Cadotte, MW Caldeira, MC Chaneton, EJ D'Antonio, CM Fay, PA Firn, J Hagenah, N Harpole, WS Iribarne, O Kirkman, KP Knops, JMH La Pierre, KJ Laungani, R Leakey, ADB McCulley, RL Moore, JL Pascual, J Borer, ET AF Flores-Moreno, Habacuc Reich, Peter B. Lind, Eric M. Sullivan, Lauren L. Seabloom, Eric W. Yahdjian, Laura MacDougall, Andrew S. Reichmann, Lara G. Alberti, Juan Baez, Selene Bakker, Jonathan D. Cadotte, Marc W. Caldeira, Maria C. Chaneton, Enrique J. D'Antonio, Carla M. Fay, Philip A. Firn, Jennifer Hagenah, Nicole Harpole, W. Stanley Iribarne, Oscar Kirkman, Kevin P. Knops, Johannes M. H. La Pierre, Kimberly J. Laungani, Ramesh Leakey, Andrew D. B. McCulley, Rebecca L. Moore, Joslin L. Pascual, Jesus Borer, Elizabeth T. TI Climate modifies response of non-native and native species richness to nutrient enrichment SO PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Article DE climate variability; non-native species; NutNet; nutrient enrichment; species abundance; species richness ID RELATIVE GROWTH-RATE; INVASIVE PLANTS; SOIL-NITROGEN; DIVERSITY; EXPLAIN; COMMUNITIES; COEXISTENCE; MECHANISMS; GRASSLANDS; ECOLOGY AB Ecosystem eutrophication often increases domination by non-natives and causes displacement of native taxa. However, variation in environmental conditions may affect the outcome of interactions between native and non-native taxa in environments where nutrient supply is elevated. We examined the interactive effects of eutrophication, climate variability and climate average conditions on the success of native and non-native plant species using experimental nutrient manipulations replicated at 32 grassland sites on four continents. We hypothesized that effects of nutrient addition would be greatest where climate was stable and benign, owing to reduced niche partitioning. We found that the abundance of non-native species increased with nutrient addition independent of climate; however, nutrient addition increased non-native species richness and decreased native species richness, with these effects dampened in warmer or wetter sites. Eutrophication also altered the time scale in which grassland invasion responded to climate, decreasing the importance of long-term climate and increasing that of annual climate. Thus, climatic conditions mediate the responses of native and non-native flora to nutrient enrichment. Our results suggest that the negative effect of nutrient addition on native abundance is decoupled from its effect on richness, and reduces the time scale of the links between climate and compositional change. C1 [Flores-Moreno, Habacuc; Lind, Eric M.; Sullivan, Lauren L.; Seabloom, Eric W.; Borer, Elizabeth T.] Univ Minnesota, Dept Ecol Evolut & Behav, St Paul, MN 55108 USA. [Flores-Moreno, Habacuc; Reich, Peter B.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Reich, Peter B.] Univ Western Sydney, Hawkesbury Inst Environm, Penrith, NSW 2751, Australia. [Yahdjian, Laura; Chaneton, Enrique J.] Univ Buenos Aires, IFEVA CONICET, RA-1417 Buenos Aires, DF, Argentina. [Yahdjian, Laura; Chaneton, Enrique J.] Univ Buenos Aires, Fac Agron, RA-1417 Buenos Aires, DF, Argentina. [MacDougall, Andrew S.] Univ Guelph, Dept Integrat Biol, Guelph, ON N1G 2W1, Canada. [Reichmann, Lara G.; Fay, Philip A.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Alberti, Juan; Iribarne, Oscar; Pascual, Jesus] Univ Nacl Mar del Plata, Inst Invest Marinas & Costeras IIMyC, CONICET, RA-7600 Mar Del Plata, Buenos Aires, Argentina. [Baez, Selene] Consorcio Desarrollo Sostenible Ecoreg Andina CON, Quito, Ecuador. [Bakker, Jonathan D.] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Cadotte, Marc W.] Univ Toronto Scarborough, Dept Biol Sci, 1265 Mil Trail, Toronto, ON M1C 2M2, Canada. [Caldeira, Maria C.] Univ Lisbon, Inst Super Agron, Ctr Estudos Florestais, P-1349017 Lisbon, Portugal. [D'Antonio, Carla M.] Univ Calif Santa Barbara, Environm Studies Program, Santa Barbara, CA 93106 USA. [Firn, Jennifer] Queensland Univ Technol, Sch Earth Environm & Biol Sci, Brisbane, Qld 4001, Australia. [Hagenah, Nicole; Kirkman, Kevin P.] Univ KwaZulu Natal, Sch Life Sci, Pietermaritzburg, South Africa. [Harpole, W. Stanley] UFZ Helmholtz Ctr Environm Res, Dept Physiol Divers, Permoserstr 15, D-04318 Leipzig, Germany. [Harpole, W. Stanley] German Ctr Integrat Biodivers Res iDiv, Deutsch Pl 5e, D-04103 Leipzig, Germany. [Harpole, W. Stanley] Univ Halle Wittenberg, Kirchtor 1, D-06108 Halle, Saale, Germany. [Knops, Johannes M. H.] Univ Nebraska, Sch Biol Sci, Lincoln, NE 68588 USA. [La Pierre, Kimberly J.] Univ Calif Berkeley, Dept Integrat Biol, Berkeley, CA 94720 USA. [Laungani, Ramesh] Doane Coll, Dept Biol, Crete, NE 68333 USA. [Leakey, Andrew D. B.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Leakey, Andrew D. B.] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. [McCulley, Rebecca L.] Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA. [Moore, Joslin L.] Monash Univ, Sch Biol Sci, Clayton, Vic 3800, Australia. RP Flores-Moreno, H (reprint author), Univ Minnesota, Dept Ecol Evolut & Behav, St Paul, MN 55108 USA.; Flores-Moreno, H (reprint author), Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. EM hfloresm@umn.edu RI Caldeira, Maria/D-4107-2013; Leakey, Andrew/Q-9889-2016; OI Caldeira, Maria/0000-0002-3586-8526; Leakey, Andrew/0000-0001-6251-024X; Lind, Eric/0000-0003-3051-7724; Borer, Elizabeth/0000-0003-2259-5853; La Pierre, Kimberly/0000-0001-7056-4547 FU Institute on the Environment [DG-0001-13]; Swiss International Cooperation (SDC) [DDC/CG/CC/CONDESAN PA0042-0011-0031 7F-07991.01]; Proyecto Prometeo; SENESCYT; Ecuador; National Science Foundation Research Coordination Network [NSF-DEB-1042132]; Long Term Ecological Research programmes [NSF412 DEB-1234162] FX H.F.M. thanks the Institute on the Environment (DG-0001-13) for funding. S.B. thanks the Swiss International Cooperation (SDC, https://www.eda.admin.ch/sdc) for funding the CIMA project for the establishment of the Pichincha long-term monitoring site conducted by CONDESAN (grant no. DDC/CG/CC/CONDESAN PA0042-0011-0031 7F-07991.01), and the Proyecto Prometeo, SENESCYT, Ecuador. This work was generated using data from the Nutrient Network (http://nutnet.org) experiment, supported at the site scale by individual researchers. Coordination and data management were supported by funding to E. Borer and E. Seabloom from the National Science Foundation Research Coordination Network (NSF-DEB-1042132), Long Term Ecological Research (NSF412 DEB-1234162 to Cedar Creek LTER) programmes, and the Institute on the Environment (DC-0001-13). NR 51 TC 2 Z9 2 U1 16 U2 29 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8436 EI 1471-2970 J9 PHILOS T R SOC B JI Philos. Trans. R. Soc. B-Biol. Sci. PD MAY 19 PY 2016 VL 371 IS 1694 AR 20150273 DI 10.1098/rstb.2015.0273 PG 9 WC Biology SC Life Sciences & Biomedicine - Other Topics GA DL8MQ UT WOS:000375896500007 ER PT J AU Lord, NP Plimpton, RL Sharkey, CR Suvorov, A Lelito, JP Willardson, BM Bybee, SM AF Lord, Nathan P. Plimpton, Rebecca L. Sharkey, Camilla R. Suvorov, Anton Lelito, Jonathan P. Willardson, Barry M. Bybee, Seth M. TI A cure for the blues: opsin duplication and subfunctionalization for short-wavelength sensitivity in jewel beetles (Coleoptera: Buprestidae) SO BMC EVOLUTIONARY BIOLOGY LA English DT Article DE Insect vision; Opsins; Agrilus planipennis; Emerald ash borer; Pest species; RNA-seq; Transcriptome; Evolution ID AMINO-ACID SUBSTITUTIONS; MULTIPLE SEQUENCE ALIGNMENT; ACTIVE FIREFLIES COLEOPTERA; PROTEIN-COUPLED RECEPTORS; SUNBURST DIVING BEETLE; RNA-SEQ DATA; SPECTRAL SENSITIVITY; COMPOUND EYE; COLOR-VISION; HELICONIUS BUTTERFLIES AB Background: Arthropods have received much attention as a model for studying opsin evolution in invertebrates. Yet, relatively few studies have investigated the diversity of opsin proteins that underlie spectral sensitivity of the visual pigments within the diverse beetles (Insecta: Coleoptera). Previous work has demonstrated that beetles appear to lack the short-wavelength-sensitive (SWS) opsin class that typically confers sensitivity to the "blue" region of the light spectrum. However, this is contrary to established physiological data in a number of Coleoptera. To explore potential adaptations at the molecular level that may compensate for the loss of the SWS opsin, we carried out an exploration of the opsin proteins within a group of beetles (Buprestidae) where short-wave sensitivity has been demonstrated. RNA-seq data were generated to identify opsin proteins from nine taxa comprising six buprestid species (including three male/female pairs) across four subfamilies. Structural analyses of recovered opsins were conducted and compared to opsin sequences in other insects across the main opsin classes-ultraviolet, short-wavelength, and long-wavelength. Results: All nine buprestids were found to express two opsin copies in each of the ultraviolet and long-wavelength classes, contrary to the single copies recovered in all other molecular studies of adult beetle opsin expression. No SWS opsin class was recovered. Furthermore, the male Agrilus planipennis (emerald ash borer-EAB) expressed a third LWS opsin at low levels that is presumed to be a larval copy. Subsequent homology and structural analyses identified multiple amino acid substitutions in the UVS and LWS copies that could confer short-wavelength sensitivity. Conclusions: This work is the first to compare expressed opsin genes against known electrophysiological data that demonstrate multiple peak sensitivities in Coleoptera. We report the first instance of opsin duplication in adult beetles, which occurs in both the UVS and LWS opsin classes. Through structural comparisons of known insect opsins, we suggest that opsin duplication and amino acid variation within the chromophore binding pocket explains sensitivity in the short-wavelength portion of the visible light spectrum in these species. These findings are the first to reveal molecular complexity of the color vision system within beetles. C1 [Lord, Nathan P.; Sharkey, Camilla R.; Suvorov, Anton; Bybee, Seth M.] Brigham Young Univ, Dept Biol, 4102 LSB, Provo, UT 84602 USA. [Plimpton, Rebecca L.; Willardson, Barry M.] Brigham Young Univ, Dept Chem & Biochem, C100 BNSN, Provo, UT 84602 USA. [Lelito, Jonathan P.] US Anim & Plant Hlth Inspect Serv, USDA, Plant Protect & Quarantine, Emerald Ash Borer Program, 5936 Ford Court Suite 200, Brighton, MI 48116 USA. RP Lord, NP (reprint author), Brigham Young Univ, Dept Biol, 4102 LSB, Provo, UT 84602 USA. EM bothriderid@gmail.com OI Sharkey, Camilla/0000-0003-3269-6025 FU National Science Foundation Division of Environmental Biology (NSF DEB) [1265714] FX This work was supported by a National Science Foundation Division of Environmental Biology (NSF DEB) grant [#1265714 to SMB]. NR 111 TC 5 Z9 5 U1 7 U2 17 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2148 J9 BMC EVOL BIOL JI BMC Evol. Biol. PD MAY 18 PY 2016 VL 16 AR 107 DI 10.1186/s12862-016-0674-4 PG 17 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA DL9UN UT WOS:000375988600002 PM 27193495 ER PT J AU Page, AF Cseke, LJ Minocha, R Turlapati, SA Podila, GK Ulanov, A Li, Z Minocha, SC AF Page, Andrew F. Cseke, Leland J. Minocha, Rakesh Turlapati, Swathi A. Podila, Gopi K. Ulanov, Alexander Li, Zhong Minocha, Subhash C. TI Genetic manipulation of putrescine biosynthesis reprograms the cellular transcriptome and the metabolome SO BMC PLANT BIOLOGY LA English DT Article DE Genetic manipulation; Metabolome; Microarrays; Ornithine decarboxylase; Polyamines; Populus; Transcriptome ID POPLAR CELLS; CDNA MICROARRAY; ELEVATED CO2; ARGININE-BIOSYNTHESIS; STRESS RESPONSES; PATHWAY GENES; POPULUS; POLYAMINE; EXPRESSION; ARABIDOPSIS AB Background: With the increasing interest in metabolic engineering of plants using genetic manipulation and gene editing technologies to enhance growth, nutritional value and environmental adaptation, a major concern is the potential of undesirable broad and distant effects of manipulating the target gene or metabolic step in the resulting plant. A comprehensive transcriptomic and metabolomic analysis of the product may shed some useful light in this regard. The present study used these two techniques with plant cell cultures to analyze the effects of genetic manipulation of a single step in the biosynthesis of polyamines because of their well-known roles in plant growth, development and stress responses. Results: The transcriptomes and metabolomes of a control and a high putrescine (HP) producing cell line of poplar (Populus nigra x maximowiczii) were compared using microarrays and GC/MS. The HP cells expressed an ornithine decarboxylase transgene and accumulated several-fold higher concentrations of putrescine, with only small changes in spermidine and spermine. The results show that up-regulation of a single step in the polyamine biosynthetic pathway (i.e. ornithine -> putrescine) altered the expression of a broad spectrum of genes; many of which were involved in transcription, translation, membrane transport, osmoregulation, shock/stress/wounding, and cell wall metabolism. More than half of the 200 detected metabolites were significantly altered (p <= 0.05) in the HP cells irrespective of sampling date. The most noteworthy differences were in organic acids, carbohydrates and nitrogen-containing metabolites. Conclusions: The results provide valuable information about the role of polyamines in regulating nitrogen and carbon use pathways in cell cultures of high putrescine producing transgenic cells of poplar vs. their low putrescine counterparts. The results underscore the complexity of cellular responses to genetic perturbation of a single metabolic step related to nitrogen metabolism in plants. Combined with recent studies from our lab, where we showed that higher putrescine production caused an increased flux of glutamate into ornithine concurrent with enhancement in glutamate production via additional nitrogen and carbon assimilation, the results from this study provide guidance in designing transgenic plants with increased nitrogen use efficiency, especially in plants intended for non-food/feed applications (e.g. increased biomass production for biofuels). C1 [Page, Andrew F.; Turlapati, Swathi A.; Minocha, Subhash C.] Univ New Hampshire, Dept Biol Sci, Durham, NH 03824 USA. [Cseke, Leland J.; Podila, Gopi K.] Univ Alabama, Dept Biol Sci, Huntsville, AL 35899 USA. [Minocha, Rakesh; Turlapati, Swathi A.] US Forest Serv, USDA, No Res Stn, Durham, NH 03824 USA. [Ulanov, Alexander; Li, Zhong] Univ Illinois, Metabol Ctr, Roy J Carver Biotechnol Ctr, Champaign, IL 61801 USA. RP Minocha, SC (reprint author), Univ New Hampshire, Dept Biol Sci, Durham, NH 03824 USA. EM sminocha@unh.edu FU New Hampshire Agricultural Experiment Station; USDA National Institute of Food and Agriculture [NH00062, NH00076-M] FX Partial funding was provided by the New Hampshire Agricultural Experiment Station. This is Scientific Contribution Number 2466. This work was supported by the USDA National Institute of Food and Agriculture (McIntire-Stennis) Project (NH00062 and NH00076-M). NR 77 TC 0 Z9 0 U1 16 U2 22 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD MAY 18 PY 2016 VL 16 AR 113 DI 10.1186/s12870-016-0796-2 PG 15 WC Plant Sciences SC Plant Sciences GA DL9IY UT WOS:000375957400001 PM 27188293 ER PT J AU Wang, L Csallany, AS Kerr, BJ Shurson, GC Chen, C AF Wang, Lei Csallany, A. Saari Kerr, Brian J. Shurson, Gerald C. Chen, Chi TI Kinetics of Forming Aldehydes in Frying Oils and Their Distribution in French Fries Revealed by LC-MS-Based Chemometrics SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE aldehydes; chemometrics; LC-MS; frying oil; French fries ID LIPID-PEROXIDATION PRODUCTS; LINOLENIC ACID; THERMAL INTERACTION; TOXIC ALDEHYDE; FOOD; TRANSFORMATION; MALONALDEHYDE; TEMPERATURE; MECHANISMS; CHEMISTRY AB In this study, the kinetics of aldehyde formation in heated frying oils was characterized by 2-hydrazinoquinoline derivatization, liquid chromatography-mass spectrometry (LC-MS) analysis, principal component analysis (PCA), and hierarchical cluster analysis (HCA). The aldehydes contributing to time-dependent separation of heated soybean oil (HSO) in a PCA model were grouped by the HCA into three clusters (A1, A2, and B) on the basis of their kinetics and fatty acid precursors. The increases of 4-hydroxynonenal (4-HNE) and the A2-to-B ratio in HSO were well-correlated with the duration of thermal stress. Chemometric and quantitative analysis of three frying oils (soybean, corn, and canola oils) and French fry extracts further supported the associations between aldehyde profiles and fatty acid precursors and also revealed that the concentrations of pentanal, hexanal, acrolein, and the A2-to-B ratio in French fry extracts were more comparable to their values in the frying oils than other unsaturated aldehydes. All of these results suggest the roles of specific aldehydes or aldehyde clusters as novel markers of the lipid oxidation status for frying oils or fried foods. C1 [Wang, Lei; Csallany, A. Saari; Chen, Chi] Univ Minnesota, Dept Food Sci & Nutr, St Paul, MN 55108 USA. [Shurson, Gerald C.] Univ Minnesota, Dept Anim Sci, St Paul, MN 55108 USA. [Kerr, Brian J.] USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA. RP Chen, C (reprint author), Univ Minnesota, Dept Food Sci & Nutr, St Paul, MN 55108 USA. EM chichen@umn.edu FU Agricultural Experiment Station from the United States Department of Agriculture (USDA) [MIN-18-092] FX This research was partially supported by the Agricultural Experiment Station project MIN-18-092 (C.C.) from the United States Department of Agriculture (USDA). NR 37 TC 1 Z9 1 U1 13 U2 23 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAY 18 PY 2016 VL 64 IS 19 BP 3881 EP 3889 DI 10.1021/acs.jafc.6b01127 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DM4QB UT WOS:000376330700017 PM 27128101 ER PT J AU Naoumkina, M Thyssen, GN Fang, DD Hinchliffe, DJ Florane, CB Jenkins, JN AF Naoumkina, Marina Thyssen, Gregory N. Fang, David D. Hinchliffe, Doug J. Florane, Christopher B. Jenkins, Johnie N. TI Small RNA sequencing and degradome analysis of developing fibers of short fiber mutants Ligon-lintles-1 (Li-1) and-2 (Li-2) revealed a role for miRNAs and their targets in cotton fiber elongation SO BMC GENOMICS LA English DT Article DE Cotton; Fiber; Elongation; miRNA; Degradome; Li-1 and Li-2 mutations ID GOSSYPIUM-HIRSUTUM L.; DIFFERENTIALLY EXPRESSED GENES; LIGON LINTLESS-2 MUTANT; GENOME-WIDE ANALYSIS; PARALLEL ANALYSIS; MESSENGER-RNA; UPLAND COTTON; TRANSCRIPTION FACTORS; ARABIDOPSIS-THALIANA; MICRORNA REGULATION AB Background: The length of cotton fiber is an important agronomic trait that directly affects the quality of yarn and fabric. Understanding the molecular basis of fiber elongation would provide a means for improvement of fiber length. Ligon-lintless-1 (Li-1) and -2 (Li-2) are monogenic and dominant mutations that result in an extreme reduction in the length of lint fiber on mature seeds. In a near-isogenic state with wild type cotton these two short fiber mutants provide an effective model system to study the mechanisms of fiber elongation. Plant miRNAs regulate many aspects of growth and development. However, the mechanism underlying the miRNA-mediated regulation of fiber development is largely unknown. Results: Small RNA libraries constructed from developing fiber cells of the short fiber mutants Li-1 and Li-2 and their near-isogenic wild type lines were sequenced. We identified 24 conservative and 147 novel miRNA families with targets that were detected through degradome sequencing. The distribution of the target genes into functional categories revealed the largest set of genes were transcription factors. Expression profiles of 20 miRNAs were examined across a fiber developmental time course in wild type and short fiber mutations. We conducted correlation analysis between miRNA transcript abundance and the length of fiber for 11 diverse Upland cotton lines. The expression patterns of 4 miRNAs revealed significant negative correlation with fiber lengths of 11 cotton lines. Conclusions: Our results suggested that the mutations have changed the regulation of miRNAs expression during fiber development. Further investigations of differentially expressed miRNAs in the Li-1 and Li-2 mutants will contribute to better understanding of the regulatory mechanisms of cotton fiber development. Four miRNAs negatively correlated with fiber length are good candidates for further investigations of miRNA regulation of important genotype dependent fiber traits. Thus, our results will contribute to further studies on the role of miRNAs in cotton fiber development and will provide a tool for fiber improvement through molecular breeding. C1 [Naoumkina, Marina; Fang, David D.; Florane, Christopher B.] USDA ARS, Cotton Fiber Biosci Res Unit, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [Thyssen, Gregory N.; Hinchliffe, Doug J.] USDA ARS, Cotton Chem & Utilizat Res Unit, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [Jenkins, Johnie N.] USDA ARS, Genet & Sustainable Agr Res Unit, 810 Highway 12 East, Mississippi State, MS 39762 USA. RP Naoumkina, M (reprint author), USDA ARS, Cotton Fiber Biosci Res Unit, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM marina.naoumkina@ars.usda.gov FU USDA-ARS CRIS project [6054-21000-017-00D]; Cotton Incorporated project [12-210] FX This project was financially supported by the USDA-ARS CRIS project # 6054-21000-017-00D and Cotton Incorporated project # 12-210. We greatly appreciate the contributions of Crista Madison from Cotton Chemistry and Utilization Research Unit for assistance with sample collection and nucleic acid extraction and Dr. Linghe Zeng for planting 11 cotton lines in Stoneville, MS. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture that is an equal opportunity provider and employer. NR 75 TC 0 Z9 0 U1 4 U2 9 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD MAY 17 PY 2016 VL 17 AR 360 DI 10.1186/s12864-016-2715-1 PG 15 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DM4ZR UT WOS:000376356500001 PM 27184029 ER PT J AU Rodriguez-Celma, J Lattanzio, G Villarroya, D Gutierrez-Carbonell, E Ceballos-Laita, L Rencoret, J Gutierrez, A del Rio, JC Grusak, MA Abadia, A Abadia, J Lopez-Millan, AF AF Rodriguez-Celma, Jorge Lattanzio, Giuseppe Villarroya, Dido Gutierrez-Carbonell, Elain Ceballos-Laita, Laura Rencoret, Jorge Gutierrez, Ana del Rio, Jose C. Grusak, Michael A. Abadia, Anunciacion Abadia, Javier Lopez-Millan, Ana-Flor TI Effects of Fe deficiency on the protein profiles and lignin composition of stem tissues from Medicago truncatula in absence or presence of calcium carbonate SO JOURNAL OF PROTEOMICS LA English DT Article DE Iron; Lignin; Microscopy; Stem; Petiole; Proteomics; Two-dimensional gel electrophoresis ID ARABIDOPSIS ROOTS REVEALS; PEACH PRUNUS-PERSICA; STRATEGY I PLANTS; IRON-DEFICIENCY; XYLEM SAP; CALCAREOUS SOIL; SUGAR-BEET; CHLOROSIS; BICARBONATE; RESPONSES AB Iron deficiency is a yield-limiting factor with major implications for crop production, especially in soils with high CaCO3. Because stems are essential for the delivery of nutrients to the shoots, the aim of this work was to study the effects of Fe deficiency on the stem proteome of Medicago truncatula. Two-dimensional electrophoresis separation of stem protein extracts resolved 276 consistent spots in the whole experiment. Iron deficiency in absence or presence of CaCO3 caused significant changes in relative abundance in 10 and 31 spots, respectively, and 80% of them were identified by mass spectrometry. Overall results indicate that Fe deficiency by itself has a mild effect on the stem proteome, whereas Fe deficiency in the presence of CaCO3 has a stronger impact and causes changes in a larger number of proteins, including increases in stress and protein metabolism related proteins not observed in the absence of CaCO3. Both treatments resulted in increases in cell wall related proteins, which were more intense in the presence of CaCO3. The increases induced by Fe-deficiency in the lignin per protein ratio and changes in the lignin monomer composition, assessed by pyrolysis-gas chromatography mass spectrometry and microscopy, respectively, further support the existence of cell wall alterations. Biological significance: In spite of being essential for the delivery of nutrients to the shoots, our knowledge of stem responses to nutrient deficiencies is very limited. The present work applies 2-DE techniques to unravel the response of this understudied tissue to Fe deficiency. Proteomics data, complemented with mineral, lignin and microscopy analyses, indicate that stems respond to Fe deficiency by increasing stress and defense related proteins, probably in response of mineral and osmotic unbalances, and eliciting significant changes in cell wall composition. The changes observed are likely to ultimately affect solute transport and distribution to the leaves. (C) 2016 Elsevier B.V. All rights reserved. C1 [Rodriguez-Celma, Jorge; Lattanzio, Giuseppe; Villarroya, Dido; Gutierrez-Carbonell, Elain; Ceballos-Laita, Laura; Abadia, Anunciacion; Abadia, Javier] Aula Dei Expt Stn CSIC, Plant Nutr Dept, POB 13034, E-50080 Zaragoza, Spain. [Rencoret, Jorge; Gutierrez, Ana; del Rio, Jose C.] Inst Recursos Nat & Agrobiol Sevilla CSIC, Reina Mercedes 10, E-41012 Seville, Spain. [Grusak, Michael A.; Lopez-Millan, Ana-Flor] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. [Rodriguez-Celma, Jorge] John Innes Ctr Plant Sci Res, Colney Lane, Norwich NR4 7UH, Norfolk, England. [Rodriguez-Celma, Jorge] Univ E Anglia, Colney Lane, Norwich NR4 7UH, Norfolk, England. RP Lopez-Millan, AF (reprint author), Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM lopezmil@bcm.edu RI Abadia, Javier/B-8804-2008; del Rio, Jose/I-8325-2012; Rodriguez-Celma, Jorge/E-9513-2012; Mangan, Rachel/A-8824-2008; RENCORET, JORGE/E-1747-2013; Abadia, Anunciacion/A-7474-2010; OI Abadia, Javier/0000-0001-5470-5901; del Rio, Jose/0000-0002-3040-6787; Rodriguez-Celma, Jorge/0000-0002-7967-5136; Mangan, Rachel/0000-0002-8788-9214; RENCORET, JORGE/0000-0003-2728-7331; Abadia, Anunciacion/0000-0003-3609-7070; Gutierrez, Ana/0000-0002-8823-9029 FU Spanish Ministry of Science and Competitiveness (MINECO, FEDER) [AGL2012-31988, AGL2011-25379, AGL2013-42175-R]; Aragon Government (group) [A03]; US Department of Agriculture, Agricultural Research Service [58-6250-0-008]; I3P-CSIC; FPI-MINECO; JAE-PRE-CSIC; JAE-DOC-CSIC; European Social Fund FX Work supported by the Spanish Ministry of Science and Competitiveness (MINECO; projects AGL2012-31988, AGL2011-25379 and AGL2013-42175-R, co-financed by FEDER), the Aragon Government (group A03), and the US Department of Agriculture, Agricultural Research Service (under Agreement number 58-6250-0-008 to MAG). Support was obtained by contracts I3P-CSIC (JRC), FPI-MINECO (GL and LC-L), JAE-PRE-CSIC (EG-C) and JAE-DOC-CSIC (JR), co-financed by the European Social Fund. Authors thank A. Calvino for assistance in growing and harvesting plants. The contents of this publication do not necessarily reflect the views or policies of the US Department of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government. NR 66 TC 1 Z9 1 U1 8 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1874-3919 EI 1876-7737 J9 J PROTEOMICS JI J. Proteomics PD MAY 17 PY 2016 VL 140 BP 1 EP 12 DI 10.1016/j.jprot.2016.03.017 PG 12 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA DM9PC UT WOS:000376697200001 PM 27045941 ER PT J AU Bajgain, P Rouse, MN Tsilo, TJ Macharia, GK Bhavani, S Jin, Y Anderson, JA AF Bajgain, Prabin Rouse, Matthew N. Tsilo, Toi J. Macharia, Godwin K. Bhavani, Sridhar Jin, Yue Anderson, James A. TI Nested Association Mapping of Stem Rust Resistance in Wheat Using Genotyping by Sequencing SO PLOS ONE LA English DT Article ID F-SP TRITICI; ADULT-PLANT RESISTANCE; QUANTITATIVE TRAIT LOCI; RACE UG99; AESTIVUM L.; BREAD WHEAT; DURUM-WHEAT; IDENTIFICATION; REGISTRATION; MARKERS AB We combined the recently developed genotyping by sequencing (GBS) method with joint mapping (also known as nested association mapping) to dissect and understand the genetic architecture controlling stem rust resistance in wheat (Triticum aestivum). Ten stem rust resistant wheat varieties were crossed to the susceptible line LMPG-6 to generate F-6 recombinant inbred lines. The recombinant inbred line populations were phenotyped in Kenya, South Africa, and St. Paul, Minnesota, USA. By joint mapping of the 10 populations, we identified 59 minor and medium-effect QTL (explained phenotypic variance range of 1% -20%) on 20 chromosomes that contributed towards adult plant resistance to North American Pgt races as well as the highly virulent Ug99 race group. Fifteen of the 59 QTL were detected in multiple environments. No epistatic relationship was detected among the QTL. While these numerous small-to medium-effect QTL are shared among the families, the founder parents were found to have different allelic effects for the QTL. Fourteen QTL identified by joint mapping were also detected in single-population mapping. As these QTL were mapped using SNP markers with known locations on the physical chromosomes, the genomic regions identified with QTL could be explored more in depth to discover candidate genes for stem rust resistance. The use of GBS-derived de novo SNPs in mapping resistance to stem rust shown in this study could be used as a model to conduct similar marker-trait association studies in other plant species. C1 [Bajgain, Prabin] Purdue Univ, Dept Agron, 915 West State St, W Lafayette, IN 47907 USA. [Bajgain, Prabin; Anderson, James A.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Rouse, Matthew N.; Jin, Yue] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Rouse, Matthew N.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Tsilo, Toi J.] Agr Res Council, Small Grain Inst, ZA-9700 Bethlehem, Free State, South Africa. [Macharia, Godwin K.] Kenya Agr & Livestock Res Org, Njoro, Kenya. [Bhavani, Sridhar] Int Maize & Wheat Improvement Ctr CIMMYT, ICRAF House,United Nations Ave, Nairobi, Kenya. RP Bajgain, P (reprint author), Purdue Univ, Dept Agron, 915 West State St, W Lafayette, IN 47907 USA.; Bajgain, P; Anderson, JA (reprint author), Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. EM bajga002@umn.edu; ander319@umn.edu RI Rouse, Matthew/G-5474-2011 FU United States Department of Agriculture, Agriculture and Food Research Initiative; Borlaug Global Rust Initiative Durable Rust Resistance in Wheat Project; Bill & Melinda Gates Foundation; UK Department for International Development; University of Minnesota Genomics Center; Minnesota Supercomputing Institute; Microbial & Plant Genomics Institute; Kenya Agricultural and Livestock Research Organization; Ethiopia Institute of Agricultural Research; Agricultural Research Council of South Africa; Anderson Wheat Lab; [2011-68002-30029] FX Funding for this work was provided by the United States Department of Agriculture, Agriculture and Food Research Initiative and 2011-68002-30029 (Triticeae Coordinated Agricultural Project http://www.triticeaecap.org/), and the Borlaug Global Rust Initiative Durable Rust Resistance in Wheat Project (administered by Cornell University with a grant from the Bill & Melinda Gates Foundation), and the UK Department for International Development.; We thank University of Minnesota Genomics Center, Minnesota Supercomputing Institute, the Microbial & Plant Genomics Institute, Kenya Agricultural and Livestock Research Organization, Ethiopia Institute of Agricultural Research, Agricultural Research Council of South Africa, USDA-ARS Cereal Disease Laboratory personnel, and Anderson Wheat Lab for their help and support during various phases of the project. Funding for this work was provided by the United States Department of Agriculture, Agriculture and Food Research Initiative and 2011-68002-30029 (Triticeae Coordinated Agricultural Project), and the Borlaug Global Rust Initiative Durable Rust Resistance in Wheat Project (administered by Cornell University with a grant from the Bill & Melinda Gates Foundation), and the UK Department for International Development. NR 91 TC 2 Z9 2 U1 10 U2 22 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 17 PY 2016 VL 11 IS 5 AR e0155760 DI 10.1371/journal.pone.0155760 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DM3YJ UT WOS:000376282300067 PM 27186883 ER PT J AU Sun, Z Liu, Z Zhou, W Jin, HA Liu, H Zhou, AM Zhang, AJ Wang, MQ AF Sun, Ze Liu, Zhuang Zhou, Wen Jin, Huanan Liu, Hao Zhou, Aiming Zhang, Aijun Wang, Man-Qun TI Temporal interactions of plant - insect - predator after infection of bacterial pathogen on rice plants SO SCIENTIFIC REPORTS LA English DT Article ID WHITE-BACKED PLANTHOPPER; MEDIATED INTERACTIONS; SOGATELLA-FURCIFERA; NILAPARVATA-LUGENS; INDUCED RESISTANCE; SYSTEMIC RESISTANCE; VOLATILE EMISSIONS; BROWN PLANTHOPPER; FUNGAL-INFECTION; BLAST FUNGUS AB Pathogenic infection on plants may affect interactions of host-plants with their herbivores, as well as the herbivores with their predators. In this study, the effects of infection by pathogenic bacterium Xanthomonas oryzae pv. oryzae (Xoo), which causes a vascular disease in rice, on rice plants and consequent interactions with a rice herbivore, brown rice planthopper (BPH) Nilaparvata lugens, and its major predator, Cyrtorhinus lividipennis, were investigated. The results showed that the rice plants exhibited increased resistance to BPH only at 3 d post-inoculation of Xoo, while the Xoo infection did not affect the development and fecundity of BPH. BPH exhibited a higher preference to Xoo infected rice plants, whereas C. lividipennis preferred the Xoo infected rice plants after BPH fed, but preferred healthy rice plants without BPH fed. Volatile organic compounds emitted from Xoo rice were significantly higher than those from healthy rice plants, Xoo infection on BPH fed plants caused rice plants to emit more the herbivore-induced plant volatiles, while all of these changes correlated to the temporal dimension. These results demonstrated that Xoo infection significantly influenced the interactions of rice plants with two non-vectors, BPH and its predator, although these effects exhibited in a temporal pattern after infection. C1 [Sun, Ze; Liu, Zhuang; Zhou, Wen; Jin, Huanan; Liu, Hao; Zhou, Aiming; Wang, Man-Qun] Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Peoples R China. [Zhang, Aijun] USDA ARS, Invas Insect Biocontrol & Behav Lab, BARC West, Beltsville, MD 20705 USA. RP Wang, MQ (reprint author), Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Peoples R China. EM mqwang@mail.hzau.edu.cn FU National High Technology Research and Development Program of China (863 Program) [2014AA10A605]; Natural Science Foundation of Hubei Province [2014CFB934] FX This study was supported and funded by the National High Technology Research and Development Program of China (863 Program) (No. 2014AA10A605) and Natural Science Foundation of Hubei Province (No. 2014CFB934). NR 60 TC 0 Z9 0 U1 20 U2 24 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD MAY 17 PY 2016 VL 6 AR 26043 DI 10.1038/srep26043 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DL9RE UT WOS:000375979600001 PM 27185548 ER PT J AU Eardley, C Griswold, T AF Eardley, Connal Griswold, Terry TI Taxonomic revision of the Afrotropical bee genus Serapista Cockerell (Hymenoptera: Apoidea: Megachilidae: Megachilinae: Anthidiini) SO ZOOTAXA LA English DT Article DE Africa; pollinator; pollen collector; Sub-Saharan; taxonomy AB The Sub-Saharan bee genus Serapista Cockerell is revised. It comprises six species. Serapista pernigra Cockerell is restored as a valid species, S. aspernigra Eardley and Griswold, new species, is described from the eastern part of southern Africa and Serapista rhodesiana Mavromoustakis is synonymized with S. pernigra. Descriptions, keys for identification, distributional records and floral associations of the species of Serapista are given. C1 [Eardley, Connal] Agr Res Council, Private Bag X134, ZA-0121 Queenswood, South Africa. [Eardley, Connal] Univ Kwazulu Natal, Sch Biol & Conservat Sci, Private Bag X01, ZA-3209 Pietermaritzburg, South Africa. [Griswold, Terry] Utah State Univ, USDA ARS, Pollinating Insects Res Unit, Logan, UT 84322 USA. RP Eardley, C (reprint author), Agr Res Council, Private Bag X134, ZA-0121 Queenswood, South Africa.; Eardley, C (reprint author), Univ Kwazulu Natal, Sch Biol & Conservat Sci, Private Bag X01, ZA-3209 Pietermaritzburg, South Africa.; Griswold, T (reprint author), Utah State Univ, USDA ARS, Pollinating Insects Res Unit, Logan, UT 84322 USA. EM eardley@arc.agric.za; terry.griswold@ars.usda.gov NR 15 TC 0 Z9 0 U1 1 U2 1 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD MAY 17 PY 2016 VL 4111 IS 4 BP 334 EP 364 PG 31 WC Zoology SC Zoology GA DL9AR UT WOS:000375934100002 PM 27395094 ER PT J AU Turner, PA Griffis, TJ Baker, JM Lee, X Crawford, JT Loken, LC Venterea, RT AF Turner, P. A. Griffis, T. J. Baker, J. M. Lee, X. Crawford, J. T. Loken, L. C. Venterea, R. T. TI Regional-scale controls on dissolved nitrous oxide in the Upper Mississippi River SO GEOPHYSICAL RESEARCH LETTERS LA English DT Article ID AQUATIC ECOSYSTEMS; REGRESSION TREES; OHIO RIVER; EMISSIONS; DENITRIFICATION; N2O; WATER; NITRIFICATION; STREAM; OCEAN AB The U.S. Corn Belt is one of the most intensive agricultural regions of the world and is drained by the Upper Mississippi River (UMR), which forms one of the largest drainage basins in the U.S. While the effects of agricultural nitrate (NO3-) on water quality in the UMR have been well documented, its impact on the production of nitrous oxide (N2O) has not been reported. Using a novel equilibration technique, we present the largest data set of freshwater dissolved N2O concentrations (0.7 to 6 times saturation) and examine the controls on its variability over a 350 km reach of the UMR. Driven by a supersaturated water column, the UMR was an important atmospheric N2O source (+ 68mg N(2)ONm(-2) yr(-1)) that varies nonlinearly with the NO3- concentration. Our analyses indicated that a projected doubling of the NO3- concentration by 2050 would cause dissolved N2O concentrations and emissions to increase by about 40%. C1 [Turner, P. A.; Griffis, T. J.; Baker, J. M.; Venterea, R. T.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Baker, J. M.; Venterea, R. T.] ARS, USDA, St Paul, MN USA. [Lee, X.] Yale Univ, Sch Forestry & Environm Studies, New Haven, CT 06511 USA. [Lee, X.] Nanjing Univ Informat & Technol, Ctr Atmospher Environm, Yale Nanjing Univ Informat Sci & Technol, Nanjing, Jiangsu, Peoples R China. [Crawford, J. T.] US Geol Survey, Natl Res Program, Boulder, CO USA. [Loken, L. C.] US Geol Survey, Wisconsin Water Sci Ctr, Middleton, WI USA. [Loken, L. C.] Univ Wisconsin, Ctr Limnol, Madison, WI USA. RP Turner, PA (reprint author), Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. EM turne289@umn.edu OI Loken, Luke/0000-0003-3194-1498 FU U.S. Department of Agriculture [USDA-NIFA 2013-67019-21364]; USDA-ARS; U.S. Geological Survey's LandCarbon program FX We thank William Breiter, Michael Dolan, Mark Dornblaser, Stephen Powers, and Matt Erickson for field and laboratory assistance. This work was supported by the U.S. Department of Agriculture, grant USDA-NIFA 2013-67019-21364, USDA-ARS, and the U.S. Geological Survey's LandCarbon program. Data are hosted at http://www.biometeorology.umn.edu/research/data-archives. Any use of trade or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 55 TC 3 Z9 3 U1 15 U2 24 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0094-8276 EI 1944-8007 J9 GEOPHYS RES LETT JI Geophys. Res. Lett. PD MAY 16 PY 2016 VL 43 IS 9 BP 4400 EP 4407 DI 10.1002/2016GL068710 PG 8 WC Geosciences, Multidisciplinary SC Geology GA DP2RS UT WOS:000378339200039 ER PT J AU Leland, C Hom, J Skowronski, N Ledig, FT Krusic, PJ Cook, ER Martin-Benito, D Martin-Fernandez, J Pederson, N AF Leland, Caroline Hom, John Skowronski, Nicholas Ledig, F. Thomas Krusic, Paul J. Cook, Edward R. Martin-Benito, Dario Martin-Fernandez, Javier Pederson, Neil TI Missing Rings, Synchronous Growth, and Ecological Disturbance in a 36-Year Pitch Pine (Pinus rigida) Provenance Study SO PLOS ONE LA English DT Article ID ATLANTIC COASTAL-PLAIN; CLIMATE-CHANGE; RADIAL GROWTH; GYPSY-MOTH; GENE FLOW; POPULATIONS; DEFOLIATION; ADAPTATION; DROUGHT; PERFORMANCE AB Provenance studies are an increasingly important analog for understanding how trees adapted to particular climatic conditions might respond to climate change. Dendrochronological analysis can illuminate differences among trees from different seed sources in terms of absolute annual growth and sensitivity to external growth factors. We analyzed annual radial growth of 567 36-year-old pitch pine (Pinus rigida Mill.) trees from 27 seed sources to evaluate their performance in a New Jersey Pine Barrens provenance experiment. Unexpectedly, missing rings were prevalent in most trees, and some years-1992, 1999, and 2006-had a particularly high frequency of missing rings across the plantation. Trees from local seed sources (<55 km away from the plantation) had a significantly smaller percentage of missing rings from 1980-2009 (mean: 5.0%), relative to northernmost and southernmost sources (mean: 9.3% and 7.9%, respectively). Some years with a high frequency of missing rings coincide with outbreaks of defoliating insects or dry growing season conditions. The propensity for missing rings synchronized annual variations in growth across all trees and might have complicated the detection of potential differences in interannual variability among seed sources. Average ring width was significantly larger in seed sources from both the southernmost and warmest origins compared to the northernmost and coldest seed sources in most years. Local seed sources had the highest average radial growth. Adaptation to local environmental conditions and disturbances might have influenced the higher growth rate found in local seed sources. These findings underscore the need to understand the integrative impact of multiple environmental drivers, such as disturbance agents and climate change, on tree growth, forest dynamics, and the carbon cycle. C1 [Leland, Caroline; Cook, Edward R.; Martin-Fernandez, Javier; Pederson, Neil] Columbia Univ, Lamont Doherty Earth Observ, Palisades, NY USA. [Hom, John] US Forest Serv, USDA, No Res Stn, Newtown Sq, PA USA. [Skowronski, Nicholas] US Forest Serv, USDA, No Res Stn, Morgantown, WV USA. [Ledig, F. Thomas] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Krusic, Paul J.] Stockholm Univ, Dept Phys Geog & Quaternary Geol, S-10691 Stockholm, Sweden. [Krusic, Paul J.] Navarino Environm Observ, Messinia, Greece. [Martin-Benito, Dario] ETH, Inst Terr Ecosyst, Zurich, Switzerland. [Pederson, Neil] Harvard Univ, Harvard Forest, Petersham, MA USA. RP Leland, C (reprint author), Columbia Univ, Lamont Doherty Earth Observ, Palisades, NY USA. EM cleland@ldeo.columbia.edu FU United States Department of Agriculture Forest Service Northern Research Joint Venture Agreements [10-JV-11242306-152, 09-JV-11242306-118]; Lamont-Doherty Earth Observatory (LDEO); EKU; LDEO FX Funding was provided by the United States Department of Agriculture Forest Service Northern Research Joint Venture Agreements #10-JV-11242306-152 and #09-JV-11242306-118 between the Northern Research Station of the U.S. Forest Service, Eastern Kentucky University (EKU), and then Lamont-Doherty Earth Observatory (LDEO). EKU and LDEO provided partial funding. NR 47 TC 2 Z9 2 U1 4 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 16 PY 2016 VL 11 IS 5 AR e0154730 DI 10.1371/journal.pone.0154730 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DM3XH UT WOS:000376279500012 PM 27182599 ER PT J AU Regna, K Kurshan, PT Harwood, BN Jenkins, AM Lai, CQ Muskavitch, MAT Kopin, AS Draper, I AF Regna, Kimberly Kurshan, Peri T. Harwood, Benjamin N. Jenkins, Adam M. Lai, Chao-Qiang Muskavitch, Marc A. T. Kopin, Alan S. Draper, Isabelle TI A critical role for the Drosophila dopamine D-1-like receptor Dop1R2 at the onset of metamorphosis SO BMC DEVELOPMENTAL BIOLOGY LA English DT Article DE Development; Dopamine; GPCR; RNAi; Transcriptome ID PROTEIN-COUPLED RECEPTOR; ANTIMICROBIAL PEPTIDE GENES; HEAT-SHOCK GENES; PUPAL CUTICLE; D1 RECEPTOR; PHARMACOLOGICAL CHARACTERIZATION; FUNCTIONAL-CHARACTERIZATION; MOLECULAR CHARACTERIZATION; METABOLIC HOMEOSTASIS; TYROSINE-HYDROXYLASE AB Background: Insect metamorphosis relies on temporal and spatial cues that are precisely controlled. Previous studies in Drosophila have shown that untimely activation of genes that are essential to metamorphosis results in growth defects, developmental delay and death. Multiple factors exist that safeguard these genes against dysregulated expression. The list of identified negative regulators that play such a role in Drosophila development continues to expand. Results: By using RNAi transgene-induced gene silencing coupled to spatio/temporal assessment, we have unraveled an important role for the Drosophila dopamine 1-like receptor, Dop1R2, in development. We show that Dop1R2 knockdown leads to pre-adult lethality. In adults that escape death, abnormal wing expansion and/or melanization defects occur. Furthermore we show that salivary gland expression of this GPCR during the late larval/prepupal stage is essential for the flies to survive through adulthood. In addition to RNAi-induced effects, treatment of larvae with the high affinity D-1-like receptor antagonist flupenthixol, also results in developmental arrest, and in morphological defects comparable to those seen in Dop1R2 RNAi flies. To examine the basis for pupal lethality in Dop1R2 RNAi flies, we carried out transcriptome analysis. These studies revealed up-regulation of genes that respond to ecdysone, regulate morphogenesis and/or modulate defense/immunity. Conclusion: Taken together our findings suggest a role for Dop1R2 in the repression of genes that coordinate metamorphosis. Premature release of this inhibition is not tolerated by the developing fly. C1 [Regna, Kimberly; Jenkins, Adam M.; Muskavitch, Marc A. T.] Boston Coll, Dept Biol, Chestnut Hill, MA 02467 USA. [Kurshan, Peri T.; Harwood, Benjamin N.; Kopin, Alan S.; Draper, Isabelle] Tufts Med Ctr, Mol Cardiol Res Inst, Boston, MA 02111 USA. [Lai, Chao-Qiang] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Muskavitch, Marc A. T.] Biogen Idec Inc, Discovery Res, Cambridge, MA 02142 USA. [Kurshan, Peri T.] Stanford Univ, Dept Biol, Stanford, CA 94305 USA. RP Draper, I (reprint author), Tufts Med Ctr, Mol Cardiol Res Inst, Boston, MA 02111 USA. EM idraper@tuftsmedicalcenter.org FU NIH/NIDDK grant [R01DK070155-04]; Biology Department at Boston College; Boston College University Libraries; Provost FX This research was funded by NIH/NIDDK grant number R01DK070155-04 (Alan S. Kopin) and by the Biology Department at Boston College. The Boston College University Libraries, in collaboration with the Provost, have generously provided funding for the open access publication costs. NR 122 TC 1 Z9 1 U1 5 U2 12 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-213X J9 BMC DEV BIOL JI BMC Dev. Biol. PD MAY 16 PY 2016 VL 16 AR 15 DI 10.1186/s12861-016-0115-z PG 19 WC Developmental Biology SC Developmental Biology GA DM0WA UT WOS:000376065300001 PM 27184815 ER PT J AU Rodriguez, PA Rodriguez, EJ Norrbom, AL Arevalo, E AF Alexander Rodriguez, Pedro Rodriguez, Erick J. Norrbom, Allen L. Arevalo, Emilio TI A new species and new records of Cryptodacus (Diptera: Tephritidae) from Colombia, Bolivia and Peru SO ZOOTAXA LA English DT Article DE Neotropical; fruit flies; new host plant; new distribution records AB Cryptodacus bernardoi Rodriguez & Rodriguez, new species, is described from Colombia. It was reared from fruits of Phoradendron sp. near piperoides (Kunth) Trel. New distribution records are reported for Cryptodacus ornatus Norrbom from Colombia and Peru, for Cryptodacus trinotatus Norrbom & Korytkowski from Colombia, and for Cryptodacus obliquus Hendel from Bolivia and Peru. The female abdomen and terminalia of C. obliquus is described for the first time. The Norrbom & Korytkowski (2008)'s key to species was modified to include C. bernardoi n. sp. C1 [Alexander Rodriguez, Pedro] ICA, Lab Moscas Fruta, CI Tibaitata Km 14 Via, Bogota, Colombia. [Rodriguez, Erick J.] Florida Dept Agr & Consumer Serv, Div Plant Ind Entomol, Gainesville, FL USA. [Norrbom, Allen L.] ARS, USDA, Smithsonian Inst, Systemat Entomol Lab, POB 37012,MRC 168, Washington, DC 20013 USA. [Arevalo, Emilio] PNMF, ICA, Direcc Tecn Epidemiol & Vigilancia Fitosanitaria, Cra 41 17-81 Zona Ind Puente Aranda, Bogota, Colombia. RP Rodriguez, PA (reprint author), ICA, Lab Moscas Fruta, CI Tibaitata Km 14 Via, Bogota, Colombia.; Rodriguez, EJ (reprint author), Florida Dept Agr & Consumer Serv, Div Plant Ind Entomol, Gainesville, FL USA.; Norrbom, AL (reprint author), ARS, USDA, Smithsonian Inst, Systemat Entomol Lab, POB 37012,MRC 168, Washington, DC 20013 USA.; Arevalo, E (reprint author), PNMF, ICA, Direcc Tecn Epidemiol & Vigilancia Fitosanitaria, Cra 41 17-81 Zona Ind Puente Aranda, Bogota, Colombia. EM pedro.rodriguez@ica.gov.co; erick.rodriguez@freshfromflorida.com; allen.norrbom@ars.usda.gov; emilio.arevalo@ica.gov.co NR 6 TC 0 Z9 0 U1 1 U2 1 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD MAY 16 PY 2016 VL 4111 IS 3 BP 276 EP 290 PG 15 WC Zoology SC Zoology GA DL9AQ UT WOS:000375934000005 PM 27395090 ER PT J AU Johnson, JS Sapkota, A Lay, DC AF Johnson, Jay S. Sapkota, Avi Lay, Donald C., Jr. TI Rapid cooling after acute hyperthermia alters intestinal morphology and increases the systemic inflammatory response in pigs SO JOURNAL OF APPLIED PHYSIOLOGY LA English DT Article DE hyperthermia; recovery; pigs; inflammation; intestinal morphology ID HEAT-STRESS; BARRIER DYSFUNCTION; TIME-COURSE; HEATSTROKE; STROKE; EXPRESSION; MICE; ENDOTOXEMIA; RAT; HEAT-SHOCK-PROTEIN-70 AB The study objective was to determine the direct effects of rapid cooling after acute hyperthermia on intestinal morphology and inflammatory response in pigs. In four repetitions, male pigs (N = 36; 88.7 +/- 1.6 kg) were exposed to thermoneutrality (TN; n = 3/rep; 19.5 +/- 0.1 degrees C) for 6 h or heat stress (HS; 36.4 +/- 0.1 degrees C) for 3 h, followed by a 3-h recovery period of rapid cooling (HSRC; n = 3/rep; rapid TN exposure and ice water dousing for 1.5 h) or gradual cooling (HSGC; n = 3/rep; gradual decrease from HS to TN). Rectal (T-R) and gastrointestinal tract (T-GI) temperatures were obtained every 15 min for 6 h. In repetitions 1 and 2, blood was collected at 60 and 180 min during HS and 30 and 60 min during recovery, and then pigs were euthanized at 180 min of recovery and duodenum, ileum, and colon tissue were collected to evaluate intestinal morphology. HS increased (P < 0.01) maximum T-R (40.7 degrees C) and T-GI (41.5 degrees C) compared with TN treatment (38.9 and 39.3 degrees C, respectively). Recovery reduced T-R (P < 0.01; 0.4 degrees C) in HSRC vs. HSGC pigs, but T-GI was similar (40.7 degrees C). HSRC reduced (P < 0.01) villus height-to-crypt depth ratio in the duodenum (34%) and ileum (46%) vs. HSGC pigs. Serum LPS concentration was greater in HSRC pigs (P = 0.04; 68.5% and 52.4%, respectively) compared with TN and HSGC pigs, and TNF-alpha concentration tended to be greater (P = 0.06; 41.2%) compared with HSGC pigs during recovery. In summary, rapid cooling reduced T-R but had no effect on TGI, and this may be linked to increased intestinal damage and a systemic inflammatory response. C1 [Johnson, Jay S.; Sapkota, Avi; Lay, Donald C., Jr.] ARS, USDA, Livestock Behav Res Unit, 125 S Russell St,Rm 219C, W Lafayette, IN 47907 USA. RP Johnson, JS (reprint author), ARS, USDA, Livestock Behav Res Unit, 125 S Russell St,Rm 219C, W Lafayette, IN 47907 USA. EM jay.johnson@ars.usda.gov NR 54 TC 2 Z9 2 U1 4 U2 4 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 8750-7587 EI 1522-1601 J9 J APPL PHYSIOL JI J. Appl. Physiol. PD MAY 15 PY 2016 VL 120 IS 10 BP 1249 EP 1259 DI 10.1152/japplphysiol.00685.2015 PG 11 WC Physiology; Sport Sciences SC Physiology; Sport Sciences GA DM9NA UT WOS:000376691800017 PM 26893031 ER PT J AU Gilchrist, CA Petri, SE Schneider, BN Reichman, DJ Jiang, NN Begum, S Watanabe, K Jansen, CS Elliott, KP Burgess, SL Ma, JZ Alam, M Kabir, M Haque, R Petri, WA AF Gilchrist, Carol A. Petri, Sarah E. Schneider, Brittany N. Reichman, Daniel J. Jiang, Nona Begum, Sharmin Watanabe, Koji Jansen, Caroline S. Elliott, K. Pamela Burgess, Stacey L. Ma, Jennie Z. Alam, Masud Kabir, Mamun Haque, Rashidul Petri, William A., Jr. TI Role of the Gut Microbiota of Children in Diarrhea Due to the Protozoan Parasite Entamoeba histolytica SO JOURNAL OF INFECTIOUS DISEASES LA English DT Article DE amebiasis; Entamoeba histolytica; Prevotella copri; IgA; environmental enteropathy ID REAL-TIME PCR; BANGLADESHI INFANTS; PRESCHOOL-CHILDREN; INFECTION; AMEBIASIS; LECTIN; HOST; CRYPTOSPORIDIUM; CONSEQUENCES; MALNUTRITION AB Background. An estimated 1 million children die each year before their fifth birthday from diarrhea. Previous population-based surveys of pediatric diarrheal diseases have identified the protozoan parasite Entamoeba histolytica, the etiological agent of amebiasis, as one of the causes of moderate-to-severe diarrhea in sub-Saharan Africa and South Asia. Methods.aEuro integral We prospectively studied the natural history of E. histolytica colonization and diarrhea among infants in an urban slum of Dhaka, Bangladesh. Results.aEuro integral Approximately 80% of children were infected with E. histolytica by the age of 2 years. Fecal anti-galactose/N-acetylgalactosamine lectin immunoglobulin A was associated with protection from reinfection, while a high parasite burden and expansion of the Prevotella copri level was associated with diarrhea. Conclusions.aEuro integral E. histolytica infection was prevalent in this population, with most infections asymptomatic and diarrhea associated with both the amount of parasite and the composition of the microbiota. C1 [Gilchrist, Carol A.; Petri, Sarah E.; Schneider, Brittany N.; Reichman, Daniel J.; Jiang, Nona; Watanabe, Koji; Jansen, Caroline S.; Elliott, K. Pamela; Burgess, Stacey L.; Petri, William A., Jr.] Univ Virginia, Dept Med, Charlottesville, VA USA. [Ma, Jennie Z.] Univ Virginia, Dept Publ Hlth Sci, Div Biostat, Charlottesville, VA USA. [Ma, Jennie Z.] Univ Virginia, Sch Med, Charlottesville, VA 22908 USA. [Begum, Sharmin; Alam, Masud; Kabir, Mamun; Haque, Rashidul] Int Ctr Diarrhoeal Dis Res, GPO Box 128, Dhaka 1000, Bangladesh. [Petri, Sarah E.] Tufts Univ, Cummings Sch Vet Med, Dept Anim & Vet Sci, North Grafton, MA USA. [Reichman, Daniel J.] Emory Univ, Sch Med, Atlanta, GA 30322 USA. [Elliott, K. Pamela] ARS, USDA, Washington, DC USA. [Begum, Sharmin] Univ Calgary, Dept Biochem & Mol Biol, Bacterial Pathogenesis Res Grp, Calgary, AB T2N 1N4, Canada. RP Gilchrist, CA (reprint author), MR-6,Rm 1707,345 Crispell Dr, Charlottesville, VA 22908 USA. EM cg2p@virginia.edu FU National Institute of Allergy and Infectious Diseases [AI103536-01, AI043596] FX This work was supported by the National Institute of Allergy and Infectious Diseases (grants AI103536-01 [to C. A. G.] and AI043596 [to W. A. P.]). NR 44 TC 4 Z9 4 U1 1 U2 2 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-1899 EI 1537-6613 J9 J INFECT DIS JI J. Infect. Dis. PD MAY 15 PY 2016 VL 213 IS 10 BP 1579 EP 1585 DI 10.1093/infdis/jiv772 PG 7 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA DM8XX UT WOS:000376648900010 PM 26712950 ER PT J AU Scherer, SS D'Amato, AW Kern, CC Palik, BJ Russell, MB AF Scherer, Sawyer S. D'Amato, Anthony W. Kern, Christel C. Palik, Brian J. Russell, Matthew B. TI Long-term impacts of prescribed fire on stand structure, growth, mortality, and individual tree vigor in Pinus resinosa forests SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Great Lakes region; Forest structure; Long-term silviculture study; Pinus resinosa; Prescribed fire ID NATIONAL WILDLIFE REFUGE; MIXED-SPECIES STANDS; RED PINE; PONDEROSA PINE; LEAF-AREA; UPPER MICHIGAN; DENSITY INDEX; UNITED-STATES; MINNESOTA; USA AB Prescribed fire is increasingly being viewed as a valuable tool for mitigating the ecological consequences of long-term fire suppression within fire-adapted forest ecosystems. While the use of burning treatments in northern temperate conifer forests has at times received considerable attention, the long-term (>10 years) effects on forest structure and development have not been quantified. We describe the persistence of prescribed fire effects in a mature red pine (Pinus resinosa Ait.)-dominated forest in northern Minnesota, USA over a similar to 50 year period, as well as the relative roles of fire season and frequency in affecting individual tree and stand-level structural responses. Burning treatments were applied on 0.4 ha compartments arranged in a randomized block design with four blocks. Burning treatments crossed fire season (dormant, summer) and frequency (annual, biennial, and periodic), and include an unburned control for comparison. Treatments were applied from 1960 to 1970, with no further management interventions occurring since. Data were collected periodically from 1960 to 2014. Forest structural development trajectories were significantly altered by the application of fire treatments. Burning treatments led to lower overstory densities, lower stand basal area, and larger tree diameters when compared to the unburned control over the study period. Differences between burning treatments were less apparent suggesting that the application of burning itself rather than a particular season and/or frequency of burning drives this long-term response. Overstory tree mortality and stand growth showed little or no response to burning treatments. In addition, we detected no impact of burning on long-term overstory tree growth efficiency (based on assessments >40 years post burning) suggesting these treatments had little cumulative effect on tree vigor. Our results indicate that the effects of burning treatments on structural dynamics are not ephemeral, but rather alter stand development trajectories in the long-term. The persistent nature of these effects highlights their potential as a tool for long-lasting structural alterations in treated stands without compromising overstory tree growth and vigor. The lack of red pine recruitment throughout the duration of the study suggests that prescribed fire alone cannot regenerate this species, and that further alteration to overstory and seedbed conditions would be needed to secure new cohorts of this species. (C) 2016 Elsevier B.V. All rights reserved. C1 [Scherer, Sawyer S.; Russell, Matthew B.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [D'Amato, Anthony W.] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT 05405 USA. [Kern, Christel C.] USDA Forest Serv, No Res Stn, Rhinelander, WI 54501 USA. [Palik, Brian J.] USDA Forest Serv, No Res Stn, Grand Rapids, MN 55744 USA. RP Scherer, SS (reprint author), Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA.; Scherer, SS (reprint author), 1530 Cleveland Ave North, St Paul, MN 55108 USA. EM scher344@umn.edu FU USFS Northern Research Station; USFS State and Private Forestry Evaluation Monitoring Program; University of Minnesota Department of Forest Resources through Henry Hansen Forest Ecology Fellowship; Catherine Hill Fellowship in Forest Resources; Minnesota Agricultural Experiment Station FX We would like to thank Robert Buckman for envisioning and establishing this study in 1959 as well as Doug Kastendick and many other scientists and technicians at the US Forest Service Northern Research Station for collecting and maintaining inventory records throughout the duration of the study. Valuable field assistance in 2014 was provided by Bryten Felix. Discussion and comments from members of the University of Minnesota Silviculture and Applied Forest Ecology Lab have greatly improved the scope and direction of this work. Alessandra Bottero, Jessica Miesel, and three anonymous reviewers provided comments on an earlier draft of this paper that greatly clarified and strengthened this work. Funding which made this work possible was provided by the USFS Northern Research Station and USFS State and Private Forestry Evaluation Monitoring Program. Additional financial support was provided by the University of Minnesota Department of Forest Resources through the Henry Hansen Forest Ecology Fellowship and Catherine Hill Fellowship in Forest Resources and the Minnesota Agricultural Experiment Station. NR 71 TC 2 Z9 2 U1 13 U2 33 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAY 15 PY 2016 VL 368 BP 7 EP 16 DI 10.1016/j.foreco.2016.02.038 PG 10 WC Forestry SC Forestry GA DK8FE UT WOS:000375161800002 ER PT J AU Zhang, JW Webster, J Young, DH Fiddler, GO AF Zhang, Jianwei Webster, Jeff Young, David H. Fiddler, Gary O. TI Effect of thinning and soil treatments on Pinus ponderosa plantations: 15-year results SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Stand productivity; Whole tree thinning; Soil compaction; Sub-soiling; Fertilization; Wood chip returns ID MIXED-CONIFER STANDS; VEGETATION CONTROL; SIERRA-NEVADA; NORTHEASTERN CALIFORNIA; MANAGEMENT INTENSITY; FUEL-REDUCTION; UNITED-STATES; FOREST SOILS; DOUGLAS-FIR; TREE GROWTH AB Thinning with removal of whole trees in a plantation or natural forest stand raises two main concerns soil compaction from the ground-based machinery and nutrient depletion particularly with whole tree harvest as is often practiced for attendant fuels reduction. To address these concerns, two sets of experimental treatments were imposed in young ponderosa pine plantations. In the first set, we applied four treatments to test the effects of thinning with biomass removal using progressively more soil manipulations: (I) control, (II) thinning only with all biomass removed, (III) same as (II) but followed by sub-soiling in traffic lanes, and (IV) same as (III) but with nitrogen and phosphorus fertilization within traffic lanes prior to sub-soiling. In the second experiment set we applied four combination treatments to test the further effects of soil manipulations with wood chips and fertilizer on traffic lanes. In thinned stands: (i) he harvested trees were chipped, and spread onto traffic lanes followed by sub-soiling and rototilling, (ii) same as (i) but traffic lanes also received N and P with the chips prior to the sub-soiling, (iii) traffic lanes were sub-soiled, then thinning chips were returned to just the surface of traffic lanes, and (iv) same as (iii) but traffic lanes also received N and P fertilizer with the chips. Tree height and diameter were measured three times, starting immediately following treatments and again at 5 and 15 years post-treatment. In addition, soil bulk density was measured at 6 years and soil chemistry (C, N, and P) was measured at 6 and 16 years. Our results indicate: (1) thinning by itself with no subsoiling did not compact the soils, but increased growth rate of residual trees, although the periodic annual increment of basal area and volume was still higher in the control than other main-plot treatments; (2) neither subsoiling nor rototilling, both of which might mitigate soil compaction, enhanced tree growth; (3) short-term plantation growth was not improved with chip returns or chips with fertilization; (4) since thinning and soil treatments showed more insect damage and higher mortality, any management operations that involve cutting or damaging trees or roots should be avoided during active periods of bark beetle flight; (5) both thinning and soil treatments did not reduce carbon sequestration in the mineral soils. A lack of growth benefits from returning thinning chips, rototilling, and direct fertilization for a longer period appeals to further study. Published by Elsevier B.V. C1 [Zhang, Jianwei] USDA, Forest Serv, Pacific Southwest Res Stn, 3644 Avtech Pkwy, Redding, CA 96002 USA. [Webster, Jeff] 7205 Granada Dr, Redding, CA 96002 USA. [Young, David H.; Fiddler, Gary O.] USDA, Forest Serv, Pacif Southwest Reg, 3644 Avtech Pkwy, Redding, CA 96002 USA. RP Zhang, JW (reprint author), USDA, Forest Serv, Pacific Southwest Res Stn, 3644 Avtech Pkwy, Redding, CA 96002 USA. EM jianweizhang@fs.fed.us FU Sierra Cascade Intensive Forest Management Research Cooperative FX We thank T.H. Spear, T.M. Alves, C.S. Shestak, and others who helped in installing, maintaining, and measuring these plots during the last 15 years. The comments from Drs. Kim Mattson, Martin Ritchie, and two anonymous reviewers for improving the manuscript are greatly appreciated. Roseburg Forest Products installed these research plots. Sierra Cascade Intensive Forest Management Research Cooperative provided partial financial support. Use of trade names in this paper does not constitute endorsement by the United States Forest Service. NR 58 TC 2 Z9 2 U1 19 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAY 15 PY 2016 VL 368 BP 123 EP 132 DI 10.1016/j.foreco.2016.03.021 PG 10 WC Forestry SC Forestry GA DK8FE UT WOS:000375161800014 ER PT J AU Salis, M Laconi, M Ager, AA Alcasena, FJ Arca, B Lozano, O de Oliveira, AF Spano, D AF Salis, Michele Laconi, Maurizio Ager, Alan A. Alcasena, Fermin J. Arca, Bachisio Lozano, Olga de Oliveira, Ana Fernandes Spano, Donatella TI Evaluating alternative fuel treatment strategies to reduce wildfire losses in a Mediterranean area SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Fuel treatment strategies; Burn probability; MTT algorithm; Mediterranean basin; Wildfire exposure; Wildfire risk mitigation ID WILDLAND-URBAN INTERFACE; BOREAL FOREST LANDSCAPE; UNITED-STATES; FIRE BEHAVIOR; NE SPAIN; MANAGEMENT; RISK; PATTERNS; GROWTH; SUPPRESSION AB The goal of this work is to evaluate by a modeling approach the effectiveness of alternative fuel treatment strategies to reduce potential losses from wildfires in Mediterranean areas. We compared strategic fuel treatments located near specific human values vs random locations, and treated 3, 9 and 15% of a 68,000 ha study area located in Sardinia, Italy. The effectiveness of each fuel treatment was assessed by simulating 25,000 wildfires using the MTT fire spread algorithm. The simulations replicated severe wildfires observed around the study area, using historic weather and fuel moisture conditions (97th percentile). Wildfire exposure profiles for the study area as a whole and for locations with specific values of interest were analyzed. Results indicated significant variations in wildfire exposure among and within the fuel management strategies and treatment intensities. The simulated mitigation strategies substantially decreased the average wildfire exposure with respect to the untreated condition, and this effect was unequivocal for all strategies. Increasing the percentage of land treated improved the effectiveness of all fuel treatment strategies. The strategy based on road protection provided the highest performances for several wildfire exposure indicators. The methodology presented in this work can be applied to facilitate the design of fuel management programs and support policy decisions to address growing wildfire risk in the region. This work is one of the first applications of fire simulation modeling to evaluate fuel management effectiveness on wildfire risk mitigation in the Mediterranean areas. (C) 2016 Elsevier B.V. All rights reserved. C1 [Salis, Michele; Laconi, Maurizio; Lozano, Olga; Spano, Donatella] Univ Sassari, Dept Sci Nat & Environm Resources DIPNET, Via Nicola 9, I-07100 Sassari, Italy. [Salis, Michele; Spano, Donatella] Euromediterranean Ctr Climate Change CMCC, IAFES Div, Via Nicola 9, I-07100 Sassari, Italy. [Ager, Alan A.] USDA, Forest Serv, Rocky Mt Res Stn, Missoula Fire Sci Lab, 5775 US Highway 10W, Missoula, MT 59808 USA. [Alcasena, Fermin J.] Univ Lleida, Dept Agr & Forest Engn, Ave Rovira Roure 191, Lleida 25198, Spain. [Arca, Bachisio] CNR, Inst Biometeorol IBIMET, Traversa La Crucca 3, I-07100 Sassari, Italy. [de Oliveira, Ana Fernandes] Univ Sassari, Dept Agr, Via Nicola 9, I-07100 Sassari, Italy. RP Salis, M (reprint author), Univ Sassari, Dept Sci Nat & Environm Resources DIPNET, Via Nicola 9, I-07100 Sassari, Italy. EM miksalis@uniss.it OI SALIS, Michele/0000-0002-0499-9726; Alcasena Urdiroz, Fermin Jesus/0000-0002-2007-9487 FU MIUR - Italy/MATTM [232/2011]; "Extreme" Project (Sardinia Reg.) [CRP-25405, LR 7/2007]; "Sughera" Project (Sardinia Reg.) [Tender 2013 LR 7/2007]; "Modeling approach to evaluate fire risk and mitigation planning actions" Project (POR SARDEGNA FSE, Asse IV Capitale umano, Linea di Attivita' l.3.1) FX This work was funded by several sources including the "GEMINA" Project (MIUR - Italy/MATTM no. 232/2011), the "Extreme" Project (Sardinia Reg., CRP-25405, LR 7/2007), the "Sughera" Project (Sardinia Reg., Tender 2013 LR 7/2007) and the "Modeling approach to evaluate fire risk and mitigation planning actions" Project (POR SARDEGNA FSE 2007-2013, Asse IV Capitale umano, Linea di Attivita' l.3.1). NR 104 TC 6 Z9 6 U1 6 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAY 15 PY 2016 VL 368 BP 207 EP 221 DI 10.1016/j.foreco.2016.03.009 PG 15 WC Forestry SC Forestry GA DK8FE UT WOS:000375161800022 ER PT J AU Chaney, RL Kim, WI Yang, JE Ok, YS AF Chaney, Rufus L. Kim, Won Il Yang, Jae E. Ok, Yong Sik TI Integrated management strategies for arsenic and cadmium in rice paddy environments Preface SO GEODERMA LA English DT Editorial Material ID ACCUMULATION; ABSORPTION; TRANSPORT; SEEDLINGS; GRAIN; ZINC C1 [Chaney, Rufus L.] ARS, USDA, Crop Syst & Global Change Lab, Beltsville, MD USA. [Kim, Won Il; Yang, Jae E.] Natl Acad Agr Sci, Dept Agrofood Safety, Chem Safety Div, Wanju Gun 55365, Jeollabuk Do, South Korea. [Yang, Jae E.; Ok, Yong Sik] Kangwon Natl Univ, Coll Agr & Life Sci, Chunchon 24341, South Korea. [Ok, Yong Sik] Kangwon Natl Univ, Korea Biochar Res Ctr, Chunchon 24341, South Korea. RP Chaney, RL (reprint author), ARS, USDA, Crop Syst & Global Change Lab, Beltsville, MD USA. NR 17 TC 1 Z9 1 U1 8 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD MAY 15 PY 2016 VL 270 SI SI BP 1 EP 2 DI 10.1016/j.geoderma.2016.03.001 PG 2 WC Soil Science SC Agriculture GA DK2AG UT WOS:000374716400001 ER PT J AU Crippen, TL Sheffield, CL Byrd, JA Esquivel, JF Beier, RC Yeater, K AF Crippen, Tawni L. Sheffield, Cynthia L. Byrd, J. Allen Esquivel, Jesus F. Beier, Ross C. Yeater, Kathleen TI Poultry litter and the environment: Physiochemical properties of litter and soil during successive flock rotations and after remote site deposition SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Broiler litter; Environmental contamination; Heavy metal; Canonical correlation ID CANONICAL DISCRIMINANT-ANALYSIS; GENETIC-VARIATION; BROILER LITTER; TRACE-ELEMENTS; UNITED-STATES; MANURE; PHOSPHORUS; NITRATE; ZINC; GROUNDWATER AB The U.S. broiler meat market has grown over the past 16 years and destinations for U.S. broiler meat exports expanded to over 150 countries. This market opportunity has spurred a corresponding increase in industrialized poultry production, which due to the confined space in which high numbers of animals are housed, risks accumulating nutrients and pollutants. The purpose of this research was to determine the level of pollutants within poultry litter and the underlying soil within a production facility; and to explore the impact of spent litter deposition into the environment. The study follows a production facility for the first 2.5 years of production. It monitors the effects of successive flocks and management practices on 15 physiochemical parameters: Ca, Cu, electrical conductivity, Fe, K, Mg, Mn, moisture, Na, NO3-/N, organic matter, P, pH, S, and Zn. Litter samples were collected in-house, after clean-outs and during stockpiling. The soil before house placement, after the clean-outs and following litter stockpiling was monitored. Management practices markedly altered the physiochemical profiles of the litter in-house. A canonical discriminant analysis was used to describe the relationship between the parameters and sampling times. The litter profiles grouped into five clusters corresponding to time and management practices. The soil in-house exhibited mean increases in all physiochemical parameters (2-297 fold) except Fe, Mg, %M, and pH. The spent litter was followed after deposition onto a field for use as fertilizer. After 20 weeks, the soil beneath the litter exhibited increases in EC, Cu, K, Na, NO3-/N, %OM, P, S and Zn; while %M decreased. Understanding the impacts of industrialized poultry farms on the environment is vital as the cumulative ecological impact of this land usage could be substantial if not properly managed to reduce the risk of potential pollutant infiltration into the environment. Published by Elsevier B.V. C1 [Crippen, Tawni L.; Sheffield, Cynthia L.; Byrd, J. Allen; Esquivel, Jesus F.; Beier, Ross C.] ARS, Southern Plains Agr Res Ctr, USDA, 2881 F&B Rd, College Stn, TX 77845 USA. [Yeater, Kathleen] ARS, USDA, PA, NRRC,Off Director, 2150 Ctr Ave,Bldg D,Suite 300, Ft Collins, CO 80526 USA. RP Crippen, TL (reprint author), ARS, USDA, SPARC, 2881 F&B Rd, College Stn, TX 77845 USA. EM tc.crippen@ars.usda.gov FU USDA FX The authors thank our commercial partners. We thank Andrew Herndon, Denise Caldwell, Charles Hernandez, Laura Ripley, Toni Poole and Bob Droleskey for their help. This project was funded by the USDA. There is no commercial or financial conflict of interest. Mention of trade names, companies, or commercial products in this publication is solely for the purpose of providing specific scientific information and does not imply recommendation or endorsement of the products by the U.S. Department of Agriculture. NR 80 TC 0 Z9 0 U1 5 U2 30 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD MAY 15 PY 2016 VL 553 BP 650 EP 661 DI 10.1016/j.scitotenv.2016.02.077 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA DI0XN UT WOS:000373220700066 PM 26990075 ER PT J AU Hildebrand, A Addison, JB Kasuga, T Fan, ZL AF Hildebrand, Amanda Addison, J. Bennett Kasuga, Takao Fan, Zhiliang TI Cellobionic acid inhibition of cellobiohydrolase I and cellobiose dehydrogenase SO BIOCHEMICAL ENGINEERING JOURNAL LA English DT Article DE Biocatalysis; Cellulase; Kinetic parameters; Cellulose; Cellobionic acid; Cellobiose dehydrogenase ID PRODUCT INHIBITION; NEUROSPORA-CRASSA; PHANEROCHAETE-CHRYSOSPORIUM; CELLULOSE; CELLULASES; ENZYMES; ASSAY; ETHANOL; BIOMASS; SYSTEM AB End-product inhibition by cellobiose and glucose is a rate-limiting factor in cellulose hydrolysis by cellulases. While cellobiose and glucose inhibition have been extensively investigated, cellobionate inhibition has been minimally studied despite the discovery that accessory proteins such as cellobiose dehydrogenase (CDH) work synergistically with cellulases to form aldonic acids. The fraction of equilibrated cellobionate in the cellobiono-delta-lactone form was determined by NMR over the range of pH 4-8. To better improve the understanding of cellobiono-delta-lactone and cellobionate inhibition of cellulases, we investigate the inhibition of Trichoderma reesei cellobiohydrolase I (CBHI), one of the most extensively studied CBHs, and Neurospora crassa CDH by equilibrated cellobionate. The well-characterized inhibitor cellobiose is evaluated in parallel for comparison. The mechanisms of inhibition are determined for both inhibitors and the kinetic parameters compared using a Michaelis-Menten inhibition model. Cellobiose and cellobionate inhibition of CBHI is extended to Avicel hydrolysis, along with an investigation of those inhibitors on Accelerase 1500, a commercial cellulase preparation. (C) 2016 Elsevier B.V. All rights reserved. C1 [Hildebrand, Amanda; Fan, Zhiliang] Univ Calif Davis, Dept Biol & Agr Engn, 1 Shields Ave, Davis, CA 95616 USA. [Addison, J. Bennett] Univ Calif Davis, Nucl Magnet Resonance Facil, 1 Shields Ave, Davis, CA 95616 USA. [Kasuga, Takao] Univ Calif Davis, Dept Plant Pathol, 1 Shields Ave, Davis, CA 95616 USA. [Kasuga, Takao] ARS, USDA, Davis, CA 95616 USA. RP Fan, ZL (reprint author), Univ Calif Davis, Dept Biol & Agr Engn, 1 Shields Ave, Davis, CA 95616 USA. EM jzfan@ucdavis.edu FU Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2011-67009-20060]; EPA Star fellowship; Cota-Robles fellowship; NSF [DBIO-722538] FX This project was supported by Agriculture and Food Research Initiative Competitive Grant No. 2011-67009-20060 from the USDA National Institute of Food and Agriculture, as well as EPA Star and Cota-Robles fellowships awarded to Amanda Hildebrand. NSF DBIO-722538 provided funding for the 800 MHz Bruker Avance III NMR spectrometer. NR 36 TC 0 Z9 0 U1 6 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1369-703X EI 1873-295X J9 BIOCHEM ENG J JI Biochem. Eng. J. PD MAY 15 PY 2016 VL 109 BP 236 EP 242 DI 10.1016/j.bej.2016.01.024 PG 7 WC Biotechnology & Applied Microbiology; Engineering, Chemical SC Biotechnology & Applied Microbiology; Engineering GA DG9FC UT WOS:000372387200027 ER PT J AU Milian-Suazo, F Garcia-Casanova, L Robbe-Austerman, S Canto-Alarcon, GJ Barcenas-Reyes, I Stuber, T Rodriguez-Hernandez, E Flores-Villalva, S AF Milian-Suazo, Feliciano Garcia-Casanova, Leticia Robbe-Austerman, Suelee Jorge Canto-Alarcon, Germinal Barcenas-Reyes, Isabel Stuber, Tod Rodriguez-Hernandez, Elba Flores-Villalva, Susana TI Molecular Relationship between Strains of M. bovis from Mexico and Those from Countries with Free Trade of Cattle with Mexico SO PLOS ONE LA English DT Article ID MYCOBACTERIUM-TUBERCULOSIS COMPLEX; LENGTH-POLYMORPHISM ANALYSIS; POPULATION-STRUCTURE; ZOONOTIC ASPECTS; UNITED-KINGDOM; EPIDEMIOLOGY; ANIMALS; SPOLIGOTYPES; INFECTION; DIFFERENTIATION AB The purpose of this study was to identify relationships between spoligotypes of M. bovis from cattle in Mexico and those reported in countries with free trade of cattle with Mexico: Australia, Canada, New Zealand and the United States of America. Mexican spoligotypes were obtained from isolates collected from cattle in different parts of the country. Spoligotypes from Canada and New Zealand were obtained from different reports in the literature. Those from the United States were obtained from the database of the National Veterinary Services Laboratory in APHIS-USDA. In order to perform the analysis in a single data set, spoligotypes were all converted to binary data and classified according to www.mbovis.org or www.pasteurguadeloupe.fr:8081. Epidemiologic information included country and species infected. From 3,198 isolates, 174 different spoligotypes were obtained, 95 were orphans. Ninety one percent of the isolates came from the Unites States (n = 1,609) and Mexico (n = 1,323). Spoligotype SB0265 is shared between Canada and the United States in cattle and wildlife. Six spoligotypes, SB0673, SB0121, SB0145, SB0971, SB0140 and SB1165, were frequent in cattle and wildlife in the United States and cattle in Mexico, suggesting wide exchange of strains. Spoligotype SB0669 was found only in Mexico. Spoligotype SB0140 was the most common in Australia and the sixth in the United States and Mexico. In a phylogenetic analysis, spoligotype SB0140 appears as the oldest spoligotype in the data set, suggesting this as the ancestral spoligotype for all spoligotypes in the five countries. Some spoligotypes are shared by animals and humans, corroborating the zoonotic importance of M. bovis. C1 [Milian-Suazo, Feliciano; Garcia-Casanova, Leticia; Jorge Canto-Alarcon, Germinal; Barcenas-Reyes, Isabel] Univ Autonoma Queretaro, Fac Ciencias Nat, Queretaro, Queretaro, Mexico. [Robbe-Austerman, Suelee; Stuber, Tod] USDA, Natl Vet Serv Lab APHIS, Ames, IA USA. [Rodriguez-Hernandez, Elba; Flores-Villalva, Susana] CENID Fisiol & Mejoramiento Anim INIFAP, Queretaro, Queretaro, Mexico. RP Milian-Suazo, F (reprint author), Univ Autonoma Queretaro, Fac Ciencias Nat, Queretaro, Queretaro, Mexico. EM feliciano.milian@uaq.mx NR 39 TC 1 Z9 1 U1 2 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 12 PY 2016 VL 11 IS 5 AR e0155207 DI 10.1371/journal.pone.0155207 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DM8CQ UT WOS:000376588600091 PM 27171239 ER PT J AU Yang, CY Powell, CA Duan, YP Shatters, R Fang, JP Zhang, MQ AF Yang, Chuanyu Powell, Charles A. Duan, Yongping Shatters, Robert Fang, Jingping Zhang, Muqing TI Deciphering the Bacterial Microbiome in Huanglongbing-Affected Citrus Treated with Thermotherapy and Sulfonamide Antibiotics SO PLOS ONE LA English DT Article ID CANDIDATUS LIBERIBACTER ASIATICUS; INFECTED CITRUS; COMMUNITY STRUCTURE; HEAT-TOLERANT; SOIL; DIVERSITY; DISEASE; PLANTS; MECHANISMS; PATHOGEN AB Huanglongbing (HLB) is a serious citrus disease that threatens the citrus industry. In previous studies, sulfonamide antibiotics and heat treatment suppressed 'Candidatus Liberibacter asiaticus' (Las), but did not completely eliminate the Las. Furthermore, there are few reports studying the bacterial microbiome of HLB-affected citrus treated by heat and sulfonamide antibiotics. In this study, combinations of heat (45 degrees C or 40 degrees C) and sulfonamide treatment (sulfathiazole sodium-STZ, or sulfadimethoxine sodium-SDX) were applied to HLB-affected citrus. The bacterial microbiome of HLB-affected citrus following thermotherapy and/or chemotherapy was characterized by PhyloChip (TM) G3-based metagenomics. Our results showed that the combination of thermotherapy at 45 degrees C and chemotherapy with STZ and SDX was more effective against HLB than thermotherapy alone, chemotherapy alone, or a combination of thermotherapy at 40 degrees C and chemotherapy. The PhyloChip (TM) G3-based results indicated that 311 empirical Operational Taxonomic Units (eOTUs) were detected in 26 phyla. Cyanobacteria (18.01%) were dominant after thermo-chemotherapy. Thermotherapy at 45 degrees C decreased eOTUs (64.43%) in leaf samples, compared with thermotherapy at 40 degrees C (73.96%) or without thermotherapy (90.68%) and it also reduced bacterial family biodiversity. The eOTU in phylum Proteobacteria was reduced significantly and eOTU_28, representing "Candidatus Liberibacter," was not detected following thermotherapy at 45 degrees C. Following antibiotic treatment with SDX and STZ, there was enhanced abundance of specific eOTUs belonging to the families Streptomycetaceae, Desulfobacteraceae, Chitinophagaceae, and Xanthomonadaceae, which may be implicated in increased resistance to plant pathogens. Our study further develops an integrated strategy for combating HLB, and also provides new insight into the bacterial microbiome of HLB-affected citrus treated by heat and sulfonamide antibiotics. C1 [Yang, Chuanyu; Zhang, Muqing] Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530005, Peoples R China. [Yang, Chuanyu; Powell, Charles A.; Zhang, Muqing] Univ Florida, IFAS, Indian River Res & Educ Ctr, Ft Pierce, FL 34945 USA. [Duan, Yongping; Shatters, Robert] USDA ARS, Hort Res Lab, Ft Pierce, FL 34945 USA. [Yang, Chuanyu; Fang, Jingping; Zhang, Muqing] Fujian Agr & Forestry Univ, Coll Crop Sci, Fuzhou 350002, Peoples R China. RP Zhang, MQ (reprint author), Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530005, Peoples R China.; Zhang, MQ (reprint author), Univ Florida, IFAS, Indian River Res & Educ Ctr, Ft Pierce, FL 34945 USA.; Zhang, MQ (reprint author), Fujian Agr & Forestry Univ, Coll Crop Sci, Fuzhou 350002, Peoples R China. EM mqzhang@ufl.edu FU US Citrus Research and Development Foundation (project CRDF) [584]; Guangxi Natural Science Foundation [2013GXNSFCCB019002]; Guangxi Programs for science and technology development [347004-7] FX This work was financially supported by the US Citrus Research and Development Foundation (project CRDF#584) (http://citrusrdf.org/), the Guangxi Natural Science Foundation (project 2013GXNSFCCB019002), and the Guangxi Programs for science and technology development (project 347004-7). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 51 TC 2 Z9 2 U1 5 U2 13 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 12 PY 2016 VL 11 IS 5 AR e0155472 DI 10.1371/journal.pone.0155472 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DM8CQ UT WOS:000376588600035 PM 27171468 ER PT J AU Lien, S Koop, BF Sandve, SR Miller, JR Kent, MP Nome, T Hvidsten, TR Leong, JS Minkley, DR Zimin, A Grammes, F Grove, H Gjuvsland, A Walenz, B Hermansen, RA von Schalburg, K Rondeau, EB Di Genova, A Samy, JKA Vik, JO Vigeland, MD Caler, L Grimholt, U Jentoft, S Vage, DI de Jong, P Moen, T Baranski, M Palti, Y Smith, DR Yorke, JA Nederbragt, AJ Tooming-Klunderud, A Jakobsen, KS Jiang, XT Fan, DD Liberles, DA Vidal, R Iturra, P Jones, SJM Jonassen, I Maass, A Omholt, SW Davidson, WS AF Lien, Sigbjorn Koop, Ben F. Sandve, Simen R. Miller, Jason R. Kent, Matthew P. Nome, Torfinn Hvidsten, Torgeir R. Leong, Jong S. Minkley, David R. Zimin, Aleksey Grammes, Fabian Grove, Harald Gjuvsland, Arne Walenz, Brian Hermansen, Russell A. von Schalburg, Kris Rondeau, Eric B. Di Genova, Alex Samy, Jeevan K. A. Vik, Jon Olav Vigeland, Magnus D. Caler, Lis Grimholt, Unni Jentoft, Sissel Vage, Dag Inge de Jong, Pieter Moen, Thomas Baranski, Matthew Palti, Yniv Smith, Douglas R. Yorke, James A. Nederbragt, Alexander J. Tooming-Klunderud, Ave Jakobsen, Kjetill S. Jiang, Xuanting Fan, Dingding Liberles, David A. Vidal, Rodrigo Iturra, Patricia Jones, Steven J. M. Jonassen, Inge Maass, Alejandro Omholt, Stig W. Davidson, William S. TI The Atlantic salmon genome provides insights into rediploidization SO NATURE LA English DT Article ID MULTIPLE SEQUENCE ALIGNMENT; DUPLICATE GENE RETENTION; TRANSPOSABLE ELEMENTS; PHYLOGENETIC ANALYSIS; MAXIMUM-LIKELIHOOD; LINKAGE MAP; EVOLUTION; FISHES; SALAR; SOFTWARE AB The whole-genome duplication 80 million years ago of the common ancestor of salmonids (salmonid-specific fourth vertebrate whole-genome duplication, Ss4R) provides unique opportunities to learn about the evolutionary fate of a duplicated vertebrate genome in 70 extant lineages. Here we present a high-quality genome assembly for Atlantic salmon (Salmo salar), and show that large genomic reorganizations, coinciding with bursts of transposon-mediated repeat expansions, were crucial for the post-Ss4R rediploidization process. Comparisons of duplicate gene expression patterns across a wide range of tissues with orthologous genes from a pre-Ss4R outgroup unexpectedly demonstrate far more instances of neofunctionalization than subfunctionalization. Surprisingly, we find that genes that were retained as duplicates after the teleost-specific whole-genome duplication 320 million years ago were not more likely to be retained after the Ss4R, and that the duplicate retention was not influenced to a great extent by the nature of the predicted protein interactions of the gene products. Finally, we demonstrate that the Atlantic salmon assembly can serve as a reference sequence for the study of other salmonids for a range of purposes. C1 [Lien, Sigbjorn; Sandve, Simen R.; Kent, Matthew P.; Nome, Torfinn; Grammes, Fabian; Grove, Harald; Gjuvsland, Arne; Samy, Jeevan K. A.; Vik, Jon Olav; Vage, Dag Inge] Norwegian Univ Life Sci, Dept Anim & Aquacultural Sci, Ctr Integrat Genet CIGENE, NO-1432 As, Norway. [Koop, Ben F.; Leong, Jong S.; Minkley, David R.; von Schalburg, Kris] Univ Victoria, Dept Biol, Victoria, BC V8W 3N5, Canada. [Miller, Jason R.; Walenz, Brian; Rondeau, Eric B.; Caler, Lis] J Craig Venter Inst, 9704 Med Ctr Dr, Rockville, MD 20850 USA. [Hvidsten, Torgeir R.] Norwegian Univ Life Sci, Dept Chem Biotechnol & Food Sci, NO-1432 As, Norway. [Hvidsten, Torgeir R.] Umea Univ, Dept Plant Physiol, Umea Plant Sci Ctr, S-90187 Umea, Sweden. [Zimin, Aleksey; Yorke, James A.] Univ Maryland, Inst Phys Sci & Technol, College Pk, MD 20742 USA. [Hermansen, Russell A.] Univ Wyoming, Dept Mol Biol, Laramie, WY 82071 USA. [Hermansen, Russell A.; Liberles, David A.] Temple Univ, Ctr Computat Genet & Genom, Philadelphia, PA 19122 USA. [Hermansen, Russell A.; Liberles, David A.] Temple Univ, Dept Biol, Philadelphia, PA 19122 USA. [Di Genova, Alex; Maass, Alejandro] Univ Chile, Ctr Math Modeling, Santiago 8370456, Chile. [Di Genova, Alex; Maass, Alejandro] Univ Chile, Ctr Genome Regulat, Santiago 8370415, Chile. [Vigeland, Magnus D.] Oslo Univ Hosp, Med Genet, NO-0424 Oslo, Norway. [Vigeland, Magnus D.] Univ Oslo, NO-0424 Oslo, Norway. [Grimholt, Unni] Norwegian Vet Inst, Dept Virol, NO-0454 Oslo, Norway. [Jentoft, Sissel; Nederbragt, Alexander J.; Tooming-Klunderud, Ave; Jakobsen, Kjetill S.] Univ Oslo, Dept Biosci, Ctr Ecol & Evolutionary Synth CEES, NO-0316 Oslo, Norway. [de Jong, Pieter] CHORI, Oakland, CA 94609 USA. [Moen, Thomas] AquaGen, NO-7462 Trondheim, Norway. [Baranski, Matthew] Nofima, NO-9291 Tromso, Norway. [Palti, Yniv] ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, Kearneysville, WV 25430 USA. [Smith, Douglas R.] Beckman Genom, Danvers, MA 01923 USA. [Smith, Douglas R.] Courtagen Life Sci, Woburn, MA 01801 USA. [Jiang, Xuanting; Fan, Dingding] BGI Shenzhen, Shenzhen 518083, Peoples R China. [Vidal, Rodrigo] Univ Santiago, Dept Biol, Lab Mol Ecol Genom & Evolutionary Studies, Santiago 9170022, Chile. [Iturra, Patricia] Univ Chile, Fac Med, Santiago 8380453, Chile. [Jones, Steven J. M.] BC Canc Agcy, Genome Sci Ctr, Vancouver, BC V5Z 4S6, Canada. [Jones, Steven J. M.; Davidson, William S.] Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC V5A 1S6, Canada. [Jonassen, Inge] Univ Bergen, Dept Informat, NO-6020 Bergen, Norway. [Omholt, Stig W.] NTNU Norwegian Univ Sci & Technol, Dept Biol, Ctr Biodivers Dynam, NO-7491 Trondheim, Norway. RI Koop, Ben/A-8151-2008; Maass, Alejandro/D-5848-2012; Hvidsten, Torgeir R/A-3409-2009; OI Koop, Ben/0000-0003-0045-5200; Maass, Alejandro/0000-0002-7038-4527; Hvidsten, Torgeir R/0000-0001-6097-2539; Vik, Jon Olav/0000-0002-7778-4515; Jonassen, Inge/0000-0003-4110-0748; Nome, Torfinn/0000-0003-1659-132X; Nederbragt, Alexander/0000-0001-5539-0999 FU Research Council of Norway (NFR); Norwegian Seafood Research Fund; Genome BC; Chilean Economic Development Agency - CORFO and InnovaChile Committee; Marine Harvest; AquaGen; Cermaq; Salmobreed; NFR [208481/F50, 226266, 225181, 221734/030]; Natural Sciences and Engineering Research Council (NSERC), Canada; University of Victoria FX The International Cooperation to Sequence the Atlantic Salmon Genome (ICSASG) was funded by the following organizations: Research Council of Norway (NFR: http://www.rcn.no), Norwegian Seafood Research Fund (http://www.fhf.no/hot-topics/about-fhf), Genome BC (http://www.genomebc.ca), The Chilean Economic Development Agency - CORFO and InnovaChile Committee (http://www.english.corfo.cl), Marine Harvest (http://www.marineharvest.no), AquaGen (http://www.aquagen.no), Cermaq (http://www.cermaq.com) and Salmobreed (http://www.salmobreedno). Additional funding for bioinformatics and statistical support at CIGENE-NMBU was provided by NFR grants 208481/F50, 226266, 225181 and 221734/030. Funding for RNA-seq and most of the repeat analysis was provided by Natural Sciences and Engineering Research Council (NSERC), Canada. NSERC also provided funding for the analyses and assemblies generated at University of Victoria throughout the project. We acknowledge the help of S. Karoliussen, M. Arnyasi, R. Martinsen Anestad and I. Johansson Schneider at CIGENE-NMBU for generating salmon and rainbow trout genotypes, and G. Gao, National Center for Cool and Cold Water Aquaculture, ARS-USDA for generating rainbow trout genotypes We also acknowledge K. Beeson and H. Baden-Tilson at the J. Craig Venter Institute for library construction and Illumina sequencing. Bioinformatic analyses were performed using resources at the Orion Computing Cluster at CIGENE-NMBU, the Norwegian metacenter for computational science (under project nn4653k), computing resources at University of Victoria partly provided by Compute Canada, and NLHPC-Chile. NR 74 TC 30 Z9 30 U1 47 U2 80 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0028-0836 EI 1476-4687 J9 NATURE JI Nature PD MAY 12 PY 2016 VL 533 IS 7602 BP 200 EP + DI 10.1038/nature17164 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DM0BJ UT WOS:000376007200040 PM 27088604 ER PT J AU Douds, DD Wilson, DO Seidel, R Ziegler-Ulsh, C AF Douds, David D. Wilson, David O. Seidel, Rita Ziegler-Ulsh, Christine TI A method to minimize the time needed for formation of mycorrhizas in sweet corn seedlings for outplanting using AM fungus inoculum. produced on-farm SO SCIENTIA HORTICULTURAE LA English DT Article DE Organic agriculture; Arbuscular mycorrhizal fungi; Biofertilizer; On-farm inoculum production ID HYPHAL GROWTH; PHOSPHORUS-NUTRITION; SPORE GERMINATION; MAIZE SEEDLINGS; COLD TOLERANCE; ROOT; SOIL; TEMPERATURE; WHEAT; INFECTION AB A preliminary greenhouse growth phase prior to outplanting allows for earlier stand establishment and the greater profits that early-harvested crops can receive. Inoculation of these crops with arbuscular mycorrhizal [AM] fungi would help combat the effects of low soil temperatures upon phosphorus uptake and establishment of mycorrhizas in the field. Significant establishment of mycorrhizas on roots of sweet corn (Zea mays L.) in the greenhouse is constrained by the approximate two week residence time. Experiments were conducted to examine the benefits of a one week incubation of inoculated potting media at greenhouse temperatures prior to sowing Z. mays seeds upon subsequent development of mycorrhizas. Resulting levels of colonization of roots were contrasted to those of seedlings grown in media that received a 4 degrees C incubation to inhibit spore germination prior to sowing seeds. Levels of AM fungus colonization were compared to those of other seedlings grown in media with a range of inoculum densities. Pre-incubation of inoculated potting media in the greenhouse prior to sowing significantly increased both percentage root length colonized and total root length colonized by AM fungi 2-5 fold at the 9-11 day sampling period over that in media incubated in the cold room prior to sowing. Comparison to colonization levels in plants exposed to a range of inoculum densities showed that warm incubation increased the effective inoculum density an average of 80%. Pre-incubation in the greenhouse of AM fungus inoculated compartmented flats of potting media proved to be an effective way to enhance formation of mycorrhizas and should prove to be an effective tool to enhance the effective potency of inoculated potting media. Published by Elsevier B.V. C1 [Douds, David D.] USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Wilson, David O.; Seidel, Rita; Ziegler-Ulsh, Christine] Rodale Inst, 611 Siegfriedale Rd, Kutztown, PA 19530 USA. [Wilson, David O.] Kings Agriseeds Inc, 60 North Ronks Rd,Suite K, Ronks, PA 17572 USA. [Seidel, Rita] Berks Cty Conservat Dist, 1238 Cty Welf Rd,Suite 200, Leesport, PA 19533 USA. [Ziegler-Ulsh, Christine] USDA NRCS, Berks Cty Agr Ctr, 1238 Cty Welf Rd,Suite 220, Leesport, PA 19533 USA. RP Douds, DD (reprint author), USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM david.douds@ars.usda.gov; davidwilson@kingsagriseeds.com; rita.seidel@berkscd.com; Christine.ZieglerUlsh@pa.usda.gov NR 54 TC 0 Z9 0 U1 9 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD MAY 12 PY 2016 VL 203 BP 62 EP 68 DI 10.1016/j.scienta.2016.03.015 PG 7 WC Horticulture SC Agriculture GA DL0QI UT WOS:000375337200009 ER PT J AU Fu, SM Shao, J Zhou, CY Hartung, JS AF Fu, Shimin Shao, Jonathan Zhou, Changyong Hartung, John S. TI Transcriptome analysis of sweet orange trees infected with 'Candidatus Liberibacter asiaticus' and two strains of Citrus Tristeza Virus SO BMC GENOMICS LA English DT Article DE Huanglongbing; Gfold; Host-pathogen interactions ID CELLULOSE-SYNTHASE-LIKE; GENE-EXPRESSION; PLANT DEFENSE; HUANGLONGBING DISEASE; CIRCADIAN-RHYTHMS; CELL-DEATH; SECONDARY METABOLITES; ARABIDOPSIS-THALIANA; ZINC TRANSPORTER; GREENING DISEASE AB Background: Huanglongbing (HLB) and tristeza, are diseases of citrus caused by a member of the alpha-proteobacteria, 'Candidatus Liberibacter asiaticus' (CaLas), and Citrus tristeza virus (CTV) respectively. HLB is a devastating disease, but CTV strains vary from very severe to very mild. Both CaLas and CTV are phloem-restricted. The CaLas-B232 strain and CTV-B6 cause a wide range of severe and similar symptoms. The mild strain CTV-B2 doesn't induce significant symptoms or damage to plants. Results: Transcriptome profiles obtained through RNA-seq revealed 611, 404 and 285 differentially expressed transcripts (DETs) after infection with CaLas-B232, CTV-B6 and CTV-B2. These DETs were components of a wide range of pathways involved in circadian rhythm, cell wall modification and cell organization, as well as transcription factors, transport, hormone response and secondary metabolism, signaling and stress response. The number of transcripts that responded to both CTV-B6 and CaLas-B232 was much larger than the number of transcripts that responded to both strains of CTV or to both CTV-B2 and CaLas-B232. A total of 38 genes were assayed by RT-qPCR and the correlation coefficients between Gfold and RT-qPCR were 0.82, 0.69, 0.81 for sweet orange plants infected with CTV-B2, CTV-B6 and CaLas-B232, respectively. Conclusions: The number and composition of DETs reflected the complexity of symptoms caused by the pathogens in established infections, although the leaf tissues sampled were asymptomatic. There were greater similarities between the sweet orange in response to CTV-B6 and CaLas-B232 than between the two CTV strains, reflecting the similar physiological changes caused by both CTV-B6 and CaLas-B232. The circadian rhythm system of plants was perturbed by all three pathogens, especially by CTV-B6, and the ion balance was also disrupted by all three pathogens, especially by CaLas-B232. Defense responses related to cell wall modification, transcriptional regulation, hormones, secondary metabolites, kinases and stress were activated by all three pathogens but with different patterns. The transcriptome profiles of Citrus sinensis identified host genes whose expression is affected by the presence of a pathogen in the phloem without producing symptoms (CTV-B2), and host genes whose expression leads to induction of symptoms in the plant (CTV-B6, CaLas-B232). C1 [Fu, Shimin; Zhou, Changyong] Southwest Univ, Coll Plant Protect, Citrus Res Inst, Chongqing, Peoples R China. [Fu, Shimin; Shao, Jonathan; Hartung, John S.] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD USA. [Fu, Shimin] Lingnan Normal Univ, Zhanjian, Peoples R China. RP Zhou, CY (reprint author), Southwest Univ, Coll Plant Protect, Citrus Res Inst, Chongqing, Peoples R China.; Hartung, JS (reprint author), USDA ARS, Mol Plant Pathol Lab, Beltsville, MD USA. EM zhoucy@cric.cn; john.hartung@ars.usda.gov FU China Scholarship Council, Southwest University; United States Department of Agriculture-Agricultural Research Service FX The research was supported by China Scholarship Council, Southwest University and United States Department of Agriculture-Agricultural Research Service. The authors thank Cristina Paul for excellent plant care without which the study would not have been possible. NR 117 TC 0 Z9 0 U1 15 U2 22 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD MAY 11 PY 2016 VL 17 AR 349 DI 10.1186/s12864-016-2663-9 PG 18 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DM4VP UT WOS:000376345500001 PM 27169471 ER EF