FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Cardenas-Garcia, S Dunwoody, RP Marcano, V Diel, DG Williams, RJ Gogal, RM Brown, CC Miller, PJ Afonso, CL AF Cardenas-Garcia, Stivalis Dunwoody, Robert P. Marcano, Valerie Diel, Diego G. Williams, Robert J. Gogal, Robert M., Jr. Brown, Corrie C. Miller, Patti J. Afonso, Claudio L. TI Effects of Chicken Interferon Gamma on Newcastle Disease Virus Vaccine Immunogenicity SO PLOS ONE LA English DT Article ID RECOMBINANT SUBUNIT VACCINE; PIGEON IFN-GAMMA; DNA VACCINE; ANTIBODY-RESPONSES; IMMUNE-RESPONSE; EXPRESSION; GENES; ADJUVANTS; CLONING; INFECTION AB More effective vaccines are needed to control avian diseases. The use of chicken interferon gamma (chIFN gamma) during vaccination is a potentially important but controversial approach that may improve the immune response to antigens. In the present study, three different systems to co-deliver chIFN gamma with Newcastle disease virus (NDV) antigens were evaluated for their ability to enhance the avian immune response and their protective capacity upon challenge with virulent NDV. These systems consisted of: 1) a DNA vaccine expressing the Newcastle disease virus fusion (F) protein co-administered with a vector expressing the chIFN gamma gene for in ovo and booster vaccination, 2) a recombinant Newcastle disease virus expressing the chIFN gamma gene (rZJ1*L/IFN gamma) used as a live vaccine delivered in ovo and into juvenile chickens, and 3) the same rZJ1*L/IFN gamma virus used as an inactivated vaccine for juvenile chickens. Co-administration of chIFN gamma with a DNA vaccine expressing the F protein resulted in higher levels of morbidity and mortality, and higher amounts of virulent virus shed after challenge when compared to the group that did not receive chIFN gamma. The live vaccine system co-delivering chIFN gamma did not enhanced post-vaccination antibody response, nor improved survival after hatch, when administered in ovo, and did not affect survival after challenge when administered to juvenile chickens. The low dose of the inactivated vaccine co-delivering active chIFN gamma induced lower antibody titers than the groups that did not receive the cytokine. The high dose of this vaccine did not increase the antibody titers or antigen-specific memory response, and did not reduce the amount of challenge virus shed or mortality after challenge. In summary, regardless of the delivery system, chIFN gamma, when administered simultaneously with the vaccine antigen, did not enhance Newcastle disease virus vaccine immunogenicity. C1 [Cardenas-Garcia, Stivalis; Marcano, Valerie; Miller, Patti J.; Afonso, Claudio L.] USDA SEA, Southeast Poultry Res Lab, Athens, GA 30613 USA. [Cardenas-Garcia, Stivalis; Marcano, Valerie; Brown, Corrie C.] Univ Georgia, Coll Vet Med, Dept Vet Pathol, Athens, GA USA. [Dunwoody, Robert P.] Mercer Univ, Mem Univ Med Ctr, Sch Med, Savannah, GA USA. [Diel, Diego G.] South Dakota State Univ, Dept Vet & Biomed Sci, Brookings, SD 57007 USA. [Williams, Robert J.; Gogal, Robert M., Jr.] Univ Georgia, Coll Vet Med, Dept Biosci & Diagnost Imaging, Athens, GA USA. RP Afonso, CL (reprint author), USDA SEA, Southeast Poultry Res Lab, Athens, GA 30613 USA. EM claudio.afonso@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service [CRIS 6612-32000-064-00D] FX This research was funded by the United States Department of Agriculture, Agricultural Research Service, CRIS 6612-32000-064-00D. NR 48 TC 0 Z9 0 U1 11 U2 11 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 13 PY 2016 VL 11 IS 7 AR e0159153 DI 10.1371/journal.pone.0159153 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DQ9CP UT WOS:000379508300061 PM 27409587 ER PT J AU Gutierrez, AH Loving, C Moise, L Terry, FE Brockmeier, SL Hughes, HR Martin, WD De Groot, AS AF Gutierrez, Andres H. Loving, Crystal Moise, Leonard Terry, Frances E. Brockmeier, Susan L. Hughes, Holly R. Martin, William D. De Groot, Anne S. TI In Vivo Validation of Predicted and Conserved T Cell Epitopes in a Swine Influenza Model SO PLOS ONE LA English DT Article ID OUTBRED PIG-POPULATIONS; A VIRUSES; MOLECULAR CHARACTERIZATION; RESPIRATORY-DISEASE; VACCINE DEVELOPMENT; IMMUNE-RESPONSES; HELPER EPITOPES; HIV VACCINE; IDENTIFICATION; MICE AB Swine influenza is a highly contagious respiratory viral infection in pigs that is responsible for significant financial losses to pig farmers annually. Current measures to protect herds from infection include: inactivated whole-virus vaccines, subunit vaccines, and alpha replicon-based vaccines. As is true for influenza vaccines for humans, these strategies do not provide broad protection against the diverse strains of influenza A virus (IAV) currently circulating in U.S. swine. Improved approaches to developing swine influenza vaccines are needed. Here, we used immunoinformatics tools to identify class I and II T cell epitopes highly conserved in seven representative strains of IAV in U.S. swine and predicted to bind to Swine Leukocyte Antigen (SLA) alleles prevalent in commercial swine. Epitope-specific interferon-gamma (IFN gamma) recall responses to pooled peptides and whole virus were detected in pigs immunized with multi-epitope plasmid DNA vaccines encoding strings of class I and II putative epitopes. In a retrospective analysis of the IFN. responses to individual peptides compared to predictions specific to the SLA alleles of cohort pigs, we evaluated the predictive performance of PigMatrix and demonstrated its ability to distinguish non-immunogenic from immunogenic peptides and to identify promiscuous class II epitopes. Overall, this study confirms the capacity of PigMatrix to predict immunogenic T cell epitopes and demonstrate its potential for use in the design of epitope-driven vaccines for swine. Additional studies that match the SLA haplotype of animals with the study epitopes will be required to evaluate the degree of immune protection conferred by epitope-driven DNA vaccines in pigs. C1 [Gutierrez, Andres H.; Moise, Leonard; De Groot, Anne S.] Univ Rhode Isl, Inst Immunol & Informat, Dept Cell & Mol Biol, 825 Chalkstone Ave, Providence, RI 02908 USA. [Loving, Crystal; Brockmeier, Susan L.; Hughes, Holly R.] ARS, Virus & Prion Dis Res Unit, NADC, USDA, Ames, IA USA. [Moise, Leonard; Terry, Frances E.; Martin, William D.; De Groot, Anne S.] EpiVax Inc, Providence, RI USA. RP De Groot, AS (reprint author), Univ Rhode Isl, Inst Immunol & Informat, Dept Cell & Mol Biol, 825 Chalkstone Ave, Providence, RI 02908 USA.; De Groot, AS (reprint author), EpiVax Inc, Providence, RI USA. EM dr.annie.degroot@gmail.com OI De Groot, Annie/0000-0001-5911-1459 FU National Pork Board [12-121] FX This study was supported by the National Pork Board (www.pork.org; project #12-121) to ADG. The funder provided support in the form of salary for author AHG, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of this author are articulated in the 'author contributions' section. EpiVax Inc. did not play the role of funder in this work. WDM and FET performed the work as EpiVax employees. ADG and LM performed the work as URI employees, but because ADG and LM are both EpiVax employees, they affiliate on papers. NR 56 TC 0 Z9 0 U1 1 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 13 PY 2016 VL 11 IS 7 AR e0159237 DI 10.1371/journal.pone.0159237 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DQ9CP UT WOS:000379508300069 PM 27411061 ER PT J AU Hamrick, KS Andrews, M AF Hamrick, Karen S. Andrews, Margaret TI SNAP Participants' Eating Patterns over the Benefit Month: A Time Use Perspective SO PLOS ONE LA English DT Article ID FOOD-STAMP PARTICIPATION; WEIGHT; CYCLE; 1ST AB Individuals receiving monthly benefits through the U.S. Supplemental Nutrition Assistance Program (SNAP) often fall short of food at the end of the month and some report feelings of hunger. To investigate this situation, we used time diaries from the 2006-08 American Time Use Survey and Eating & Health Module to identify the timing of days where respondents reported no eating occurrences. Analysis includes descriptive statistics, a logit model, and a simulated benefit month. We found that SNAP participants were increasingly more likely than nonparticipants to report a day with no eating occurrences over the benefit issuance cycle. This supports the view that there is a monthly cycle in food consumption associated with the SNAP monthly benefit issuance policy. C1 [Hamrick, Karen S.; Andrews, Margaret] USDA, Econ Res Serv, Washington, DC 20024 USA. RP Hamrick, KS (reprint author), USDA, Econ Res Serv, Washington, DC 20024 USA. EM khamrick@ers.usda.gov NR 42 TC 0 Z9 0 U1 7 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 13 PY 2016 VL 11 IS 7 AR e0158422 DI 10.1371/journal.pone.0158422 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DQ9CP UT WOS:000379508300024 PM 27410962 ER PT J AU Vaughan, MM Huffaker, A Schmelz, EA Dafoe, NJ Christensen, SA McAuslane, HJ Alborn, HT Allen, LH Teal, PEA AF Vaughan, Martha M. Huffaker, Alisa Schmelz, Eric A. Dafoe, Nicole J. Christensen, Shawn A. McAuslane, Heather J. Alborn, Hans T. Allen, Leon Hartwell Teal, Peter E. A. TI Interactive Effects of Elevated [CO2] and Drought on the Maize Phytochemical Defense Response against Mycotoxigenic Fusarium verticillioides SO PLOS ONE LA English DT Article ID PLANT-PATHOGEN INTERACTIONS; ATMOSPHERIC CARBON-DIOXIDE; CLIMATE-CHANGE; ABSCISIC-ACID; JASMONIC ACID; HDMBOA-GLC; RESISTANCE; WATER; FUMONISIN; CORN AB Changes in climate due to rising atmospheric carbon dioxide concentration ([CO2]) are predicted to intensify episodes of drought, but our understanding of how these combined conditions will influence crop-pathogen interactions is limited. We recently demonstrated that elevated [CO2] alone enhances maize susceptibility to the mycotoxigenic pathogen, Fusarium verticillioides (Fv) but fumonisin levels remain unaffected. In this study we show that maize simultaneously exposed to elevated [CO2] and drought are even more susceptible to Fv proliferation and also prone to higher levels of fumonisin contamination. Despite the increase in fumonisin levels, the amount of fumonisin produced in relation to pathogen biomass remained lower than corresponding plants grown at ambient [CO2]. Therefore, the increase in fumonisin contamination was likely due to even greater pathogen biomass rather than an increase in host-derived stimulants. Drought did not negate the compromising effects of elevated [CO2] on the accumulation of maize phytohormones and metabolites. However, since elevated [CO2] does not influence the drought-induced accumulation of abscisic acid (ABA) or root terpenoid phytoalexins, the effects elevated [CO2] are negated belowground, but the stifled defense response aboveground may be a consequence of resource redirection to the roots. C1 [Vaughan, Martha M.] ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. [Huffaker, Alisa; Schmelz, Eric A.; Dafoe, Nicole J.; Christensen, Shawn A.; Alborn, Hans T.; Allen, Leon Hartwell; Teal, Peter E. A.] ARS, Chem Res Unit, Ctr Med Agr & Vet Entomol, USDA, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. [McAuslane, Heather J.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32610 USA. RP Vaughan, MM (reprint author), ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM Martha.vaughan@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service FX Research was funded by the United States Department of Agriculture, Agricultural Research Service. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 60 TC 0 Z9 0 U1 16 U2 26 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 13 PY 2016 VL 11 IS 7 AR e0159270 DI 10.1371/journal.pone.0159270 PG 24 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DQ9CP UT WOS:000379508300074 PM 27410032 ER PT J AU Elder, JR Chiok, KL Paul, NC Haldorson, G Guard, J Shah, DH AF Elder, Jacob R. Chiok, Kim Lam Paul, Narayan C. Haldorson, Gary Guard, Jean Shah, Devendra H. TI The Salmonella pathogenicity island 13 contributes to pathogenesis in streptomycin pre-treated mice but not in day-old chickens (vol 8, 16, 2016) SO Gut Pathogens LA English DT Correction C1 [Elder, Jacob R.; Chiok, Kim Lam; Paul, Narayan C.; Haldorson, Gary; Shah, Devendra H.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Shah, Devendra H.] Washington State Univ, Coll Vet Med, Paul Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. [Guard, Jean] ARS, USDA, Egg Qual & Safety Res Unit, Athens, GA 30605 USA. RP Shah, DH (reprint author), Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA.; Shah, DH (reprint author), Washington State Univ, Coll Vet Med, Paul Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. EM dshah@vetmed.wsu.edu FU NIGMS NIH HHS [T32 GM008336] NR 1 TC 0 Z9 0 U1 1 U2 1 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1757-4749 J9 GUT PATHOG JI Gut Pathogens PD JUL 12 PY 2016 VL 8 AR 35 DI 10.1186/s13099-016-0114-4 PG 2 WC Gastroenterology & Hepatology; Microbiology SC Gastroenterology & Hepatology; Microbiology GA DS9AK UT WOS:000381074000001 PM 27413396 ER PT J AU Chang, HX Yendrek, CR Caetano-Anolles, G Hartman, GL AF Chang, Hao-Xun Yendrek, Craig R. Caetano-Anolles, Gustavo Hartman, Glen L. TI Genomic characterization of plant cell wall degrading enzymes and in silico analysis of xylanses and polygalacturonases of Fusarium virguliforme SO BMC MICROBIOLOGY LA English DT Article DE Soybean; Sudden death syndrome (SDS); Fusarium virguliforme; Plant cell wall degrading enzymes (PCWDEs); Transgenic soybeans; Xylanases; Polygalacturonase; PCWDE inhibitor proteins (PIPs) ID SUDDEN-DEATH SYNDROME; DIFFERENTIAL EXPRESSION ANALYSIS; WHEAT TRANSGENIC PLANTS; INHIBITING PROTEIN; SWISS-MODEL; TAXI-III; FUNCTIONAL-ANALYSIS; ROOT RESPONSE; ACTIVE-SITE; PEAR PGIP AB Background: Plant cell wall degrading enzymes (PCWDEs) are a subset of carbohydrate-active enzymes (CAZy) produced by plant pathogens to degrade plant cell walls. To counteract PCWDEs, plants release PCWDEs inhibitor proteins (PIPs) to reduce their impact. Several transgenic plants expressing exogenous PIPs that interact with fungal glycoside hydrolase (GH) 11-type xylanases or GH28-type polygalacturonase (PG) have been shown to enhance disease resistance. However, many plant pathogenic Fusarium species were reported to escape PIPs inhibition. Fusarium virguliforme is a soilborne pathogen that causes soybean sudden death syndrome (SDS). Although the genome of F. virguliforme was sequenced, there were limited studies focused on the PCWDEs of F. virguliforme. Our goal was to understand the genomic CAZy structure of F. viguliforme, and determine if exogenous PIPs could be theoretically used in soybean to enhance resistance against F. virguliforme. Results: F. virguliforme produces diverse CAZy to degrade cellulose and pectin, similar to other necrotorphic and hemibiotrophic plant pathogenic fungi. However, some common CAZy of plant pathogenic fungi that catalyze hemicellulose, such as GH29, GH30, GH44, GH54, GH62, and GH67, were deficient in F. virguliforme. While the absence of these CAZy families might be complemented by other hemicellulases, F. virguliforme contained unique families including GH131, polysaccharide lyase (PL) 9, PL20, and PL22 that were not reported in other plant pathogenic fungi or oomycetes. Sequence analysis revealed two GH11 xylanases of F. virguliforme, FvXyn11A and FvXyn11B, have conserved residues that allow xylanase inhibitor protein I (XIP-I) binding. Structural modeling suggested that FvXyn11A and FvXyn11B could be blocked by XIP-I that serves as good candidate for developing transgenic soybeans. In contrast, one GH28 PG, FvPG2, contains an amino acid substitution that is potentially incompatible with the bean polygalacturonase-inhibitor protein II (PvPGIP2). Conclusions: Identification and annotation of CAZy provided advanced understanding of genomic composition of PCWDEs in F. virguliforme. Sequence and structural analyses of FvXyn11A and FvXyn11B suggested both xylanases were conserved in residues that allow XIP-I inhibition, and expression of both xylanases were detected during soybean roots infection. We postulate that a transgenic soybean expressing wheat XIP-I may be useful for developing root rot resistance to F. virguliforme. C1 [Chang, Hao-Xun; Caetano-Anolles, Gustavo; Hartman, Glen L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Yendrek, Craig R.] Inst Genom Biol, Urbana, IL 61801 USA. [Hartman, Glen L.] USDA ARS, Urbana, IL 61801 USA. [Hartman, Glen L.] Univ Illinois, Natl Soybean Res Ctr, 1101 W Peabody Dr, Urbana, IL 61801 USA. RP Hartman, GL (reprint author), Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA.; Hartman, GL (reprint author), USDA ARS, Urbana, IL 61801 USA.; Hartman, GL (reprint author), Univ Illinois, Natl Soybean Res Ctr, 1101 W Peabody Dr, Urbana, IL 61801 USA. EM ghartman@illinois.edu OI Chang, Hao-Xun/0000-0002-4667-7741 FU United States Department of Agriculture-Agricultural Research Service; University of Illinois FX The project was partially supported by the United States Department of Agriculture-Agricultural Research Service and the University of Illinois. NR 66 TC 0 Z9 0 U1 12 U2 17 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2180 J9 BMC MICROBIOL JI BMC Microbiol. PD JUL 12 PY 2016 VL 16 AR 147 DI 10.1186/s12866-016-0761-0 PG 12 WC Microbiology SC Microbiology GA DR4SQ UT WOS:000379893100001 PM 27405320 ER PT J AU Zhao, CH Zeng, HW Wu, RTY Cheng, WH AF Zhao, Changhui Zeng, Huawei Wu, Ryan T. Y. Cheng, Wen-Hsing TI Loss of Selenium-Binding Protein 1 Decreases Sensitivity to Clastogens and Intracellular Selenium Content in HeLa Cells SO PLOS ONE LA English DT Article ID CELLULAR GLUTATHIONE-PEROXIDASE; COLORECTAL-CANCER; DNA-DAMAGE; OVARIAN-CANCER; IN-VITRO; EXPRESSION; MICE; SURVIVAL; OVEREXPRESSION; CARCINOGENESIS AB Selenium-binding protein 1 (SBP1) is not a selenoprotein but structurally binds selenium. Loss of SBP1 during carcinogenesis usually predicts poor prognosis. Because genome instability is a hallmark of cancer, we hypothesize that SBP1 sequesters cellular selenium and sensitizes cancer cells to DNA-damaging agents. To test this hypothesis, we knocked down SBP1 expression in HeLa cervical cancer cells by employing a short hairpin RNA (shRNA) approach. Reduced sensitivity to hydrogen peroxide, paraquat and camptothecin, reactive oxygen species content, and intracellular retention of selenium after selenomethionine treatment were observed in SBP1 shRNA HeLa cells. Results from Western analyses showed that treatment of HeLa cells with selenomethionine resulted in increased SBP1 protein expression in a dose-dependent manner. Knockdown of SBP1 rendered HeLa cells increased expression of glutathione peroxidase-1 but not glutathione peroxidase-4 protein levels and accelerated migration from a wound. Altogether, SBP1 retains supplemental selenium and sensitizes HeLa cancer cells to clastogens, suggesting a new cancer treatment strategy by sequestering selenium through SBP1. C1 [Zhao, Changhui] Jilin Univ, Coll Food Sci & Engn, Dept Food Qual & Safety, Changchun 130062, Jilin Province, Peoples R China. [Zhao, Changhui; Wu, Ryan T. Y.] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Zeng, Huawei] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. [Cheng, Wen-Hsing] Mississippi State Univ, Dept Food Sci Nutr & Hlth Promot, Mississippi State, MS 39762 USA. RP Zhao, CH (reprint author), Jilin Univ, Coll Food Sci & Engn, Dept Food Qual & Safety, Changchun 130062, Jilin Province, Peoples R China.; Zhao, CH (reprint author), Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA.; Cheng, WH (reprint author), Mississippi State Univ, Dept Food Sci Nutr & Hlth Promot, Mississippi State, MS 39762 USA. EM czhao@jlu.edu.cn; wc523@msstate.edu FU USDA intramural program; Chinese Top University Graduate Student Studying Abroad Program; China Scholarship Council FX H.W. Zeng is supported by the USDA intramural program. C.Z. was partially supported by the Chinese Top University Graduate Student Studying Abroad Program, China Scholarship Council. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 42 TC 0 Z9 0 U1 3 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 12 PY 2016 VL 11 IS 7 AR e0158650 DI 10.1371/journal.pone.0158650 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DQ9CO UT WOS:000379508200014 PM 27404728 ER PT J AU Garcia-Ruiz, A Cole, JB VanRaden, PM Wiggans, GR Ruiz-Lopez, FJ Van Tassell, CP AF Garcia-Ruiz, Adriana Cole, John B. VanRaden, Paul M. Wiggans, George R. Ruiz-Lopez, Felipe J. Van Tassell, Curtis P. TI Changes in genetic selection differentials and generation intervals in US Holstein dairy cattle as a result of genomic selection SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE genomic selection; genetic improvement; Holstein; dairy cattle; generation interval ID SOMATIC-CELL SCORE; PREDICTED TRANSMITTING ABILITIES; UNITED-STATES; PRODUCTIVE LIFE; ACCURACY; FUTURE; BULLS; MERIT; IMPROVEMENT; EFFICIENCY AB Seven years after the introduction of genomic selection in the United States, it is now possible to evaluate the impact of this technology on the population. Selection differential(s) (SD) and generation interval(s) (GI) were characterized in a four-path selection model that included sire(s) of bulls (SB), sire(s) of cows (SC), dam(s) of bulls (DB), and dam(s) of cows (DC). Changes in SD over time were estimated for milk, fat, and protein yield; somatic cell score (SCS); productive life (PL); and daughter pregnancy rate (DPR) for the Holstein breed. In the period following implementation of genomic selection, dramatic reductions were seen in GI, especially the SB and SC paths. The SB GI reduced from similar to 7 y to less than 2.5 y, and the DB GI fell from about 4 y to nearly 2.5 y. SD were relatively stable for yield traits, although modest gains were noted in recent years. The most dramatic response to genomic selection was observed for the lowly heritable traits DPR, PL, and SCS. Genetic trends changed from close to zero to large and favorable, resulting in rapid genetic improvement in fertility, lifespan, and health in a breed where these traits eroded over time. These results clearly demonstrate the positive impact of genomic selection in US dairy cattle, even though this technology has only been in use for a short time. Based on the four-path selection model, rates of genetic gain per year increased from similar to 50-100% for yield traits and from threefold to fourfold for lowly heritable traits. C1 [Garcia-Ruiz, Adriana; Ruiz-Lopez, Felipe J.] Inst Nacl Invest Forestales Agr & Pecuarias, Ctr Nacl Invest Disciplinaria Fisiol & Mejoramien, Ajuchitlan 76280, Querataro, Mexico. [Garcia-Ruiz, Adriana; Cole, John B.; VanRaden, Paul M.; Wiggans, George R.; Van Tassell, Curtis P.] USDA ARS, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. RP Van Tassell, CP (reprint author), USDA ARS, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. EM curt.vantassell@ars.usda.gov RI Cole, John/J-8571-2014 OI Cole, John/0000-0003-1242-4401 FU Agricultural Research Service (ARS)-USDA Appropriated Project [1245-31000-101-00]; ARS-USDA Appropriated Project [1245-31000-104-00]; National Council of Science and Technology; National Autonomous University of Mexico; Instituto Nacional de Investigaciones Forestales Agricolas y Pecuarias Project [11402733072] FX We acknowledge the significant improvements suggested by the reviewers. We thank the Council on Dairy Cattle Breeding and the Cooperative Dairy DNA Repository for providing data used in this study. J.B.C., P.M.V., and G.R.W. were supported by Agricultural Research Service (ARS)-USDA Appropriated Project 1245-31000-101-00, "Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information." C.P.V.T. was supported by ARS-USDA Appropriated Project 1245-31000-104-00, "Enhancing Genetic Merit of Ruminants through Genome Selection and Analysis." A.G.-R. was supported by the National Council of Science and Technology and the National Autonomous University of Mexico. F.J.R.-L. and A.G.-R. were supported by Instituto Nacional de Investigaciones Forestales Agricolas y Pecuarias Project 11402733072, "Aplicacion de herramientas genomicas en el mejoramiento genetico de la fertilidad del ganado Holstein productor de leche." NR 51 TC 4 Z9 4 U1 11 U2 14 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JUL 12 PY 2016 VL 113 IS 28 BP E3995 EP E4004 DI 10.1073/pnas.1519061113 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DR1VX UT WOS:000379694100005 PM 27354521 ER PT J AU Chaudhari, SN Mukherjee, M Vagasi, AS Bi, G Rahman, MM Nguyen, CQ Paul, L Selhub, J Kipreos, ET AF Chaudhari, Snehal N. Mukherjee, Madhumati Vagasi, Alexandra S. Bi, Gaofeng Rahman, Mohammad M. Nguyen, Christine Q. Paul, Ligi Selhub, Jacob Kipreos, Edward T. TI Bacterial Folates Provide an Exogenous Signal for C. elegans Germline Stem Cell Proliferation SO DEVELOPMENTAL CELL LA English DT Article ID CAENORHABDITIS-ELEGANS; ESCHERICHIA-COLI; RECEPTOR-ALPHA; FOLIC-ACID; CANCER; TRANSPORTER; BIOLOGY; PROTEIN; PHOSPHORYLATION; DEFICIENCY AB Here we describe an in vitro primary culture system for Caenorhabditis elegans germline stem cells. This culture system was used to identify a bacterial folate as a positive regulator of germ cell proliferation. Folates are a family of B-complex vitamins that function in one-carbon metabolism to allow the de novo synthesis of amino acids and nucleosides. We show that germ cell proliferation is stimulated by the folate 10-formyl-tetrahydrofolate-Glun both in vitro and in animals. Other folates that can act as vitamins to rescue folate deficiency lack this germ cell stimulatory activity. The bacterial folate precursor dihydropteroate also promotes germ cell proliferation in vitro and in vivo, despite its inability to promote one-carbon metabolism. The folate receptor homolog FOLR-1 is required for the stimulation of germ cells by 10-formyl-tetrahydrofolate-Glun and dihydropteroate. This work defines a folate and folate-related compound as exogenous signals to modulate germ cell proliferation. C1 [Chaudhari, Snehal N.; Vagasi, Alexandra S.; Nguyen, Christine Q.; Kipreos, Edward T.] Univ Georgia, Dept Cellular Biol, Athens, GA 30602 USA. [Mukherjee, Madhumati; Rahman, Mohammad M.] Univ Georgia, Dept Genet, Athens, GA 30602 USA. [Bi, Gaofeng; Paul, Ligi; Selhub, Jacob] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Rahman, Mohammad M.] NIDDK, LCMB, Bethesda, MD 20892 USA. RP Kipreos, ET (reprint author), Univ Georgia, Dept Cellular Biol, Athens, GA 30602 USA. EM ekipreos@uga.edu FU NIH Office of Research Infrastructure Programs [P40 OD010440]; NSF [MCB-1138454]; NIH/NIGMS [R01 GM074212]; United States Department of Agriculture [51520-008-04S] FX We thank M.J. McEachern and members of the Kipreos laboratory for critical comments on the manuscript, as well as M.C. Zetka, D.B. Hausman, and S. Dougan for reagents or resources. Some C. elegans strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). This work was supported by grants from NSF (MCB-1138454) and NIH/NIGMS (R01 GM074212) (E.T.K.), and support from United States Department of Agriculture cooperative agreement 51520-008-04S (J.S.). Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the United States Department of Agriculture. NR 60 TC 0 Z9 0 U1 10 U2 10 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1534-5807 EI 1878-1551 J9 DEV CELL JI Dev. Cell PD JUL 11 PY 2016 VL 38 IS 1 BP 33 EP 46 DI 10.1016/j.devcel.2016.06.013 PG 14 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA DR7IO UT WOS:000380073400008 PM 27404357 ER PT J AU Ortega-Leon, G Thomas, DB AF Ortega-Leon, Guillermina Thomas, Donald B. TI Pseudocromata, a new genus of Ochlerini based on a new species from Ecuador (Pentatomidae: Discocephalinae) SO ZOOTAXA LA English DT Letter ID HEMIPTERA; HETEROPTERA C1 [Ortega-Leon, Guillermina] Univ Nacl Autonoma Mexico, Inst Biol, Apartado Postal 70-153, Mexico City 04510, DF, Mexico. [Thomas, Donald B.] ARS, USDA, Cattle Fever Tick Res Lab, 22675 N Moorefield Rd, Edinburg, TX 78541 USA. RP Ortega-Leon, G (reprint author), Univ Nacl Autonoma Mexico, Inst Biol, Apartado Postal 70-153, Mexico City 04510, DF, Mexico. EM ortegag@ib.unam.mx; donald.thomas@ars.usda.gov NR 5 TC 0 Z9 0 U1 0 U2 1 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD JUL 11 PY 2016 VL 4137 IS 2 BP 286 EP 290 DI 10.11646/zootaxa.4137.2.10 PG 5 WC Zoology SC Zoology GA DQ5BQ UT WOS:000379219800010 PM 27470723 ER PT J AU Swetnam, TL O'Connor, CD Lynch, AM AF Swetnam, Tyson L. O'Connor, Christopher D. Lynch, Ann M. TI Tree Morphologic Plasticity Explains Deviation from Metabolic Scaling Theory in Semi-Arid Conifer Forests, Southwestern USA SO PLOS ONE LA English DT Article ID SANTA CATALINA MOUNTAINS; SIZE DISTRIBUTIONS; ALLOMETRIC EQUATIONS; PINALENO MOUNTAINS; TROPICAL FORESTS; UNITED-STATES; CARBON STOCKS; GROWTH; MODEL; DYNAMICS AB A significant concern about Metabolic Scaling Theory (MST) in real forests relates to consistent differences between the values of power law scaling exponents of tree primary size measures used to estimate mass and those predicted by MST. Here we consider why observed scaling exponents for diameter and height relationships deviate from MST predictions across three semi-arid conifer forests in relation to: (1) tree condition and physical form, (2) the level of inter-tree competition (e.g. open vs closed stand structure), (3) increasing tree age, and (4) differences in site productivity. Scaling exponent values derived from non-linear least-squares regression for trees in excellent condition (n = 381) were above the MST prediction at the 95% confidence level, while the exponent for trees in good condition were no different than MST (n = 926). Trees that were in fair or poor condition, characterized as diseased, leaning, or sparsely crowned had exponent values below MST predictions (n = 2,058), as did recently dead standing trees (n = 375). Exponent value of the mean-tree model that disregarded tree condition (n = 3,740) was consistent with other studies that reject MST scaling. Ostensibly, as stand density and competition increase trees exhibited greater morphological plasticity whereby the majority had characteristically fair or poor growth forms. Fitting by least-squares regression biases the mean-tree model scaling exponent toward values that are below MST idealized predictions. For 368 trees from Arizona with known establishment dates, increasing age had no significant impact on expected scaling. We further suggest height to diameter ratios below MST relate to vertical truncation caused by limitation in plant water availability. Even with environmentally imposed height limitation, proportionality between height and diameter scaling exponents were consistent with the predictions of MST. C1 [Swetnam, Tyson L.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ USA. [O'Connor, Christopher D.; Lynch, Ann M.] US Forest Serv, Rocky Mt Res Stn, Missoula, MT USA. [Lynch, Ann M.] Univ Arizona, Tree Ring Res Lab, Tucson, AZ 85721 USA. RP Swetnam, TL (reprint author), Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ USA. EM tswetnam@email.arizona.edu FU USFS Region 3 Office; Coronado National Forest; Rocky Mountain Research Station, U.S. Forest Service through Research Joint Venture Agreement [07-JV-11221615-317]; University of Arizona; Nature Conservancy; Critical Zone Observatory (NSF Award) [0724958]; USDA Forest Service, Rocky Mountain Research Station [07-JV-11221615-317]; U.S. Forest Service; United States National Park Service, Valles Caldera National Preserve FX The Santa Catalina field work and TL Swetnam were funded by the USFS Region 3 Office and the Coronado National Forest. The Pinaleno Demography Project, AM Lynch, and CD O'Connor were funded by the Rocky Mountain Research Station, U.S. Forest Service through Research Joint Venture Agreement 07-JV-11221615-317, the Coronado National Forest, University of Arizona, and the Nature Conservancy. The Valles Caldera field work and TL Swetnam were funded by the Critical Zone Observatory (NSF Award #0724958).; The research, analysis and other work documented in this publication was partially funded by the USDA Forest Service, Rocky Mountain Research Station, through Agreement # 07-JV-11221615-317 in cooperation with the U.S. Forest Service, Coronado National Forest, and the United States National Park Service, Valles Caldera National Preserve; however the findings, conclusions, and views expressed are those of the authors and do not necessarily represent the views of the U.S. Forest Service or National Park Service. Additional funding and support was provided by the USFS Region 3 Office, the Coronado National Forest, and the Critical Zone Observatory (NSF Award #0724958). Pinaleno lidar data were analyzed by the USFS Remote Sensing and Application Center (RSAC): Tom Mellin, Denise Laes, and Brent Mitchell. Pinaleno plot data were collected by USFS Personnel: Craig Wilcox, and Matt Littrell; University of Arizona personnel: Don Falk, Jesse Minor, Rebecca Minor, Laura Marshall, Alex Arizpe, Josh Farella, Jacquie Dewar, Ben Schippers, Anastasia Rabin, Kyle Miller, Alicia Durnin and Andrew McIntyre. Valles Caldera plot data were collected by Scott Compton of the Valles Caldera Preserve; Jon Pelletier, Shirley Papuga, Joshua Conver, and Kristine Nelson of the University of Arizona CZO. The Santa Catalina plot data were collected by Alicia Durnham, Anastasia Rabin, Benjamin Schippers, Jacquie Dewar, Jesse Minor, Joshua Conver, Kyle Miller, and Shane Cook. NR 79 TC 0 Z9 0 U1 3 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 8 PY 2016 VL 11 IS 7 AR e0157582 DI 10.1371/journal.pone.0157582 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DR6IK UT WOS:000380005400014 PM 27391084 ER PT J AU Veltri, D Wight, MM Crouch, JA AF Veltri, Daniel Wight, Martha Malapi Crouch, Jo Anne TI SimpleSynteny: a web-based tool for visualization of microsynteny across multiple species SO NUCLEIC ACIDS RESEARCH LA English DT Article ID PLANT GENOMES; SYNTENY; HOMOLOGY; FUNGI AB Defining syntenic relationships among orthologous gene clusters is a frequent undertaking of biologists studying organismal evolution through comparative genomic approaches. With the increasing availability of genome data made possible through next-generation sequencing technology, there is a growing need for user-friendly tools capable of assessing synteny. Here we present SimpleSynteny, a new web-based platform capable of directly interrogating collinearity of local genomic neighbors across multiple species in a targeted manner. SimpleSynteny provides a pipeline for evaluating the synteny of a preselected set of gene targets across multiple organismal genomes. An emphasis has been placed on ease-of-use, and users are only required to submit FASTA files for their genomes and genes of interest. SimpleSynteny then guides the user through an iterative process of exploring and customizing genomes individually before combining them into a final high-resolution figure. Because the process is iterative, it allows the user to customize the organization of multiple contigs and incorporate knowledge from additional sources, rather than forcing complete dependence on the computational predictions. Additional tools are provided to help the user identify which contigs in a genome assembly contain gene targets and to optimize analyses of circular genomes. C1 [Veltri, Daniel; Wight, Martha Malapi; Crouch, Jo Anne] ARS, Systemat Mycol & Microbiol Lab, USDA, 10300 Baltimore Ave,Bldg 10A, Beltsville, MD 20705 USA. [Veltri, Daniel] Oak Ridge Inst Sci & Educ, ARS Res Program, MC-100-44 POB 117, Oak Ridge, TN 37831 USA. [Wight, Martha Malapi] Anim & Plant Hlth Inspect Serv, Plant Germplasm Quarantine Program, USDA, 9901 Powder Mill Rd,Bldg 580, Beltsville, MD 20705 USA. RP Crouch, JA (reprint author), ARS, Systemat Mycol & Microbiol Lab, USDA, 10300 Baltimore Ave,Bldg 10A, Beltsville, MD 20705 USA. EM joanne.crouch@ars.usda.gov OI Crouch, Jo Anne/0000-0001-6886-8090; Veltri, Daniel/0000-0002-6101-6693 FU United States Department of Agriculture (USDA)-Animal and Plant Health Inspection Service Farm Bill Program [10201, 10007]; USDA-Agricultural Research Service (ARS) [8042-22000-279-00D]; USDA-ARS Floriculture and Nursery Research Initiative [0500-00059-001]; United States Department of Energy (DOE); USDA through the Oak Ridge Institute for Science and Education ARS Research Participation Program [DOE] [DE-AC05-06OR23100]; Class of USDA-ARS Headquarters Research Associate Award; U.S. Department of Agriculture, Agricultural Research Service FX 2013-2015 United States Department of Agriculture (USDA)-Animal and Plant Health Inspection Service Farm Bill 10201 and 10007 Program funds (to J.A.C.); USDA-Agricultural Research Service (ARS) [project 8042-22000-279-00D]; USDA-ARS Floriculture and Nursery Research Initiative [project 0500-00059-001 to J.A.C.]. Inter-agency fellowship agreement between the United States Department of Energy (DOE) and the USDA through the Oak Ridge Institute for Science and Education ARS Research Participation Program Fellowship [DOE contract DE-AC05-06OR23100]; Class of 2013 USDA-ARS Headquarters Research Associate Award (to J.A.C.). Funding for open access charge: U.S. Department of Agriculture, Agricultural Research Service. NR 16 TC 2 Z9 2 U1 2 U2 6 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 EI 1362-4962 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD JUL 8 PY 2016 VL 44 IS W1 BP W41 EP W45 DI 10.1093/nar/gkw330 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA DR3FA UT WOS:000379786800008 PM 27141960 ER PT J AU Chen, TA Baranowski, T Moreno, JP O'Connor, TM Hughes, SO Baranowski, J Woehler, D Kimbro, RT Johnston, CA AF Chen, Tzu-An Baranowski, Tom Moreno, Jennette P. O'Connor, Teresia M. Hughes, Sheryl O. Baranowski, Janice Woehler, Deborah Kimbro, Rachel T. Johnston, Craig A. TI Obesity status trajectory groups among elementary school children SO BMC PUBLIC HEALTH LA English DT Article ID BODY-MASS INDEX; DEVELOPMENTAL TRAJECTORIES; SOCIOECONOMIC-STATUS; CARDIOVASCULAR-DISEASE; CHILDHOOD OVERWEIGHT; JAPANESE CHILDREN; PHYSICAL-ACTIVITY; SUMMER VACATION; RISK-FACTORS; US CHILDREN AB Background: Little is known about patterns in the transition from healthy weight to overweight or obesity during the elementary school years. This study examined whether there were distinct body mass index (BMI) trajectory groups among elementary school children, and predictors of trajectory group membership. Methods: This is a secondary analysis of 1651 elementary school children with complete biannual longitudinal data from kindergarten to the beginning of 5th grade. Heights and weights were measured by trained school nurses using standard procedures at the beginning and end of each school year for 11 consecutive assessments. Group-based trajectory clustering and multinomial logit modeling were conducted. Results: When using BMIz score, six trajectory groups were identified revealing substantial consistency in BMIz score across time. When using a categorical variable separating overweight/obese children (BMI >= 85%ile) from the rest, five developmental trajectories (persistently non-overweight/obese weight: 51.1 %; early-onset overweight/obese: 9.2 %; late-onset overweight/obese: 9.7 %; becoming healthy weight: 8.2 %; and chronically overweight/obese: 21.8 %) were identified. When using a categorical variable separating obese children (BMI >= 95%ile) from the rest, three trajectories (persistently non-obese: 74.1 %, becoming obese: 12.8 %; and chronically obese: 13.2 %) were identified. For both cutoffs (>= BMI percentile 85 % or 95 %), girls were more likely than boys to be classified in the persistently non-overweight and/or obese group (odds ratios (OR) ranged from 0.53 to 0.67); and Hispanic children and non-Hispanic Black children were more likely to be chronically overweight and/or obese than non-Hispanic White children (OR ranged from 1.57 to 2.44). Hispanic children were also more likely to become obese (OR: 1.84) than non-Hispanic White children when >= BMI percentile 95 % was used. Conclusions: Boys, Hispanic and non-Hispanic Black children were at higher risk of being overweight or obese throughout their elementary school years, supporting the need for obesity treatment. Post kindergarten and post second grade summer months were times when some children transitioned into overweight/obesity. It will be important to identify which behavioral factors (e.g., diet, physical activity, sedentary behaviors, and/or sleep) predisposed children to becoming overweight/obese, and whether these factors differ by time (Kindergarten versus second grade). If behavioral predisposing factors could be identified early, targeted obesity prevention should be offered. C1 [Chen, Tzu-An] Univ Texas Hlth Sci Ctr Houston, Ctr Translat Injury Res, 6431 Fannin St,Rm 5-212, Houston, TX 77030 USA. [Baranowski, Tom; Moreno, Jennette P.; O'Connor, Teresia M.; Hughes, Sheryl O.; Baranowski, Janice] USDA ARS, Baylor Coll Med, Childrens Nutr Res Ctr, Houston, TX USA. [Woehler, Deborah] Oliver Fdn, Houston, TX USA. [Kimbro, Rachel T.] Rice Univ, Dept Sociol, Houston, TX USA. [Johnston, Craig A.] Univ Houston, Dept Hlth & Human Performance, Houston, TX USA. RP Chen, TA (reprint author), Univ Texas Hlth Sci Ctr Houston, Ctr Translat Injury Res, 6431 Fannin St,Rm 5-212, Houston, TX 77030 USA. EM Tzu-An.Chen@uth.tmc.edu RI Dey, Kamalesh/E-6568-2017 FU Oliver Foundation FX This study was supported by a grant from The Oliver Foundation, and institutional support from the U.S. Department of Agriculture, Agricultural Research Service (Cooperative Agreement 58-6250-0-008). NR 61 TC 0 Z9 0 U1 3 U2 6 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2458 J9 BMC PUBLIC HEALTH JI BMC Public Health PD JUL 7 PY 2016 VL 16 AR 526 DI 10.1186/s12889-016-3159-x PG 12 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA DQ5PK UT WOS:000379257100003 PM 27387030 ER PT J AU Ford, KL Baumgartner, K Henricot, B Bailey, AM Foster, GD AF Ford, Kathryn L. Baumgartner, Kendra Henricot, Beatrice Bailey, Andy M. Foster, Gary D. TI A native promoter and inclusion of an intron is necessary for efficient expression of GFP or mRFP in Armillaria mellea SO SCIENTIFIC REPORTS LA English DT Article ID GREEN FLUORESCENT PROTEIN; AGROBACTERIUM-MEDIATED TRANSFORMATION; AGARICUS-BISPORUS; ROOT DISEASE; HETEROLOGOUS EXPRESSION; SCHIZOPHYLLUM-COMMUNE; COPRINOPSIS-CINEREA; GENE-EXPRESSION; RESISTANCE; PATHOGEN AB Armillaria mellea is a significant pathogen that causes Armillaria root disease on numerous hosts in forests, gardens and agricultural environments worldwide. Using a yeast-adapted pCAMBIA0380 Agrobacterium vector, we have constructed a series of vectors for transformation of A. mellea, assembled using yeast-based recombination methods. These have been designed to allow easy exchange of promoters and inclusion of introns. The vectors were first tested by transformation into basidiomycete Clitopilus passeckerianus to ascertain vector functionality then used to transform A. mellea. We show that heterologous promoters from the basidiomycetes Agaricus bisporus and Phanerochaete chrysosporium that were used successfully to control the hygromycin resistance cassette were not able to support expression of mRFP or GFP in A. mellea. The endogenous A. mellea gpd promoter delivered efficient expression, and we show that inclusion of an intron was also required for transgene expression. GFP and mRFP expression was stable in mycelia and fluorescence was visible in transgenic fruiting bodies and GFP was detectable in planta. Use of these vectors has been successful in giving expression of the fluorescent proteins GFP and mRFP in A. mellea, providing an additional molecular tool for this pathogen. C1 [Ford, Kathryn L.; Bailey, Andy M.; Foster, Gary D.] Univ Bristol, Sch Biol Sci, Life Sci Bldg,24 Tyndall Ave, Bristol BS8 1TQ, Avon, England. [Baumgartner, Kendra] Univ Calif Davis, USDA ARS, 363 Hutchison Hall,One Shields Ave, Davis, CA 95616 USA. [Henricot, Beatrice] Royal Hort Soc, Woking GU23 6QB, Surrey, England. [Henricot, Beatrice] No Res Stn, Forest Res, Roslin EH25 9SY, Midlothian, Scotland. RP Foster, GD (reprint author), Univ Bristol, Sch Biol Sci, Life Sci Bldg,24 Tyndall Ave, Bristol BS8 1TQ, Avon, England. EM Gary.Foster@bristol.ac.uk OI Bailey, Andy/0000-0002-7594-3703 FU Royal Horticultural Society; United States Department of Agriculture FX This research was funded in part by the Royal Horticultural Society and United States Department of Agriculture and was conducted under the DEFRA Plant Health Licence PHL 51046/198767/2. NR 60 TC 1 Z9 1 U1 12 U2 22 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUL 7 PY 2016 VL 6 AR 29226 DI 10.1038/srep29226 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DQ4BD UT WOS:000379147100001 PM 27384974 ER PT J AU Boue, SM Daigle, KW Chen, MH Cao, HP Heiman, ML AF Boue, Stephen M. Daigle, Kim W. Chen, Ming-Hsuan Cao, Heping Heiman, Mark L. TI Antidiabetic Potential of Purple and Red Rice (Oryza sativa L.) Bran Extracts SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE brown rice bran; purple rice bran; red rice bran; adipocytes; glucose uptake; phenolics; anthocyanins; proanthocyanidins; alpha-glucosidase; alpha-amylase ID ALPHA-GLUCOSIDASE INHIBITORS; MOUSE 3T3-L1 ADIPOCYTES; INSULIN-RESISTANCE; GRAPE SEED; ANTIOXIDATIVE ACTIVITIES; DIABETES-MELLITUS; MESSENGER-RNA; AMYLASE; PROANTHOCYANIDINS; EXPRESSION AB Pigmented rice contains anthocyanins and proanthocyanidins that are concentrated in the bran layer. In this study, we determined the phenolic, flavonoid, anthocyanin, and proanthocyanidin content of five rice bran (1 brown, 2 red, and 2 purple) extracts. Each bran extract was evaluated for inhibitory effects on alpha-amylase and alpha-glucosidase activity, two key glucosidases required for starch digestion in humans. All purple and red bran extracts inhibited alpha-glucosidase activity, however only the red rice bran extracts inhibited alpha-amylase activity. Additionally, each bran extract was examined for their ability to stimulate glucose uptake in 3T3-L1 adipocytes, a key function in glucose homeostasis. Basal glucose uptake was increased between 2.3- and 2.7-fold by exposure to the red bran extracts, and between 1.9- and 3.1-fold by exposure to the purple bran extracts. In red rice bran, the highest enzyme inhibition and glucose uptake was observed with a proanthocyanidin-enriched fraction. Both IITA red bran and IAC purple bran increased expression of GLUT]. and GLUT4 mRNA, and genes encoding insulin-signaling pathway proteins. C1 [Boue, Stephen M.; Daigle, Kim W.; Cao, Heping] ARS, Southern Reg Res Ctr, USDA, New Orleans, LA 70124 USA. [Chen, Ming-Hsuan] ARS, Dale Bumpers Natl Rice Res Ctr, USDA, 2890 Highway 130 East, Stuttgart, AR 72160 USA. [Heiman, Mark L.] Microbiome Therapeut, 11001 120th Ave, Broomfield, CO 80021 USA. RP Boue, SM (reprint author), ARS, Southern Reg Res Ctr, USDA, New Orleans, LA 70124 USA. EM steve.boue@ars.usda.gov NR 50 TC 0 Z9 0 U1 14 U2 23 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUL 6 PY 2016 VL 64 IS 26 BP 5345 EP 5353 DI 10.1021/acs.jafc.6b01909 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DQ8IZ UT WOS:000379455500007 PM 27285791 ER PT J AU Pitino, M Armstrong, CM Cano, LM Duan, YP AF Pitino, Marco Armstrong, Cheryl M. Cano, Liliana M. Duan, Yongping TI Transient Expression of Candidatus Liberibacter Asiaticus Effector Induces Cell Death in Nicotiana benthamiana SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE huanglongbing; Candidatus Liberibacter asiaticus; bacterial effectors; cell death; Nicotiana benthamiana; callose deposition; chloroplast localization ID PLANT IMMUNE-SYSTEM; DISEASE-RESISTANCE; GREENING DISEASE; CITRUS-SINENSIS; SWEET ORANGE; ARABIDOPSIS-THALIANA; GENE-EXPRESSION; INFECTION; PROTEINS; RESPONSES AB Candidatus Liberibacter asiaticus "Las is a phloem-limited bacterial plant pathogen, and the most prevalent species of Liberibacter associated with citrus huanglongbing (HLB), a devastating disease of citrus worldwide. Although, the complete sequence of the Las genome provides the basis for studying functional genomics of Las and molecular mechanisms of Las-plant interactions, the functional characterization of Las effectors remains a slow process since remains to be cultured. Like other plant pathogens, Las may deliver effector proteins into host cells and modulate a variety of host cellular functions for their infection progression. In this study, we identified 16 putative Las effectors via bioinformatics, and transiently expressed them in Nicotiana benthamiana. Diverse subcellular localization with different shapes and aggregation patterns of the effector candidates were revealed by UV- microscopy after transient expression in leaf tissue. Intriguingly, one of the 16 candidates, Las5315mp (mature protein), was localized in the chloroplast and induced cell death at 3 days post inoculation (dpi) in N. bentharniana Moreover, Las5315mp induced strong callose deposition in plant cells. This study provides new insights into the localizations and potential roles of these Las effectors in planta. C1 [Pitino, Marco; Armstrong, Cheryl M.; Duan, Yongping] ARS, US Hort Res Lab, USDA, Ft Pierce, FL 34945 USA. [Cano, Liliana M.] Univ Florida, Inst Food & Agr Sci, Dept Plant Pathol, Indian River Res & Educ Ctr, Ft Pierce, FL USA. RP Duan, YP (reprint author), ARS, US Hort Res Lab, USDA, Ft Pierce, FL 34945 USA. EM yongping.duan@aus.usda.gov RI Cano, Liliana/O-7041-2016 OI Cano, Liliana/0000-0002-7996-4181 FU U. S. Department of Agriculture; Citrus Research and Development Foundation FX We would like to thank Melissa Doud for her critical review, Carrie Vanderspool for the plants used in this study and Christina Latza for her excellent technical assistance. Funding was provided by the U. S. Department of Agriculture and Citrus Research and Development Foundation. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 84 TC 1 Z9 2 U1 13 U2 16 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JUL 6 PY 2016 VL 7 AR 982 DI 10.3389/fpls.2016.00982 PG 13 WC Plant Sciences SC Plant Sciences GA DQ1WQ UT WOS:000378991600001 PM 27458468 ER PT J AU Bevins, SN Dusek, RJ White, CL Gidlewski, T Bodenstein, B Mansfield, KG DeBruyn, P Kraege, D Rowan, E Gillin, C Thomas, B Chandler, S Baroch, J Schmit, B Grady, MJ Miller, RS Drew, ML Stopak, S Zscheile, B Bennett, J Sengl, J Brady, C Ip, HS Spackman, E Killian, ML Torchetti, MK Sleeman, JM Deliberto, TJ AF Bevins, S. N. Dusek, R. J. White, C. L. Gidlewski, T. Bodenstein, B. Mansfield, K. G. DeBruyn, P. Kraege, D. Rowan, E. Gillin, C. Thomas, B. Chandler, S. Baroch, J. Schmit, B. Grady, M. J. Miller, R. S. Drew, M. L. Stopak, S. Zscheile, B. Bennett, J. Sengl, J. Brady, Caroline Ip, H. S. Spackman, E. Killian, M. L. Torchetti, M. K. Sleeman, J. M. Deliberto, T. J. TI Widespread detection of highly pathogenic H5 influenza viruses in wild birds from the Pacific Flyway of the United States SO SCIENTIFIC REPORTS LA English DT Article ID AVIAN INFLUENZA; SUBTYPE IDENTIFICATION; INHIBITION ASSAY; MIGRATORY BIRDS; DUCKS; ASIA; INFECTION; POULTRY; KOREA AB A novel highly pathogenic avian influenza virus belonging to the H5 clade 2.3.4.4 variant viruses was detected in North America in late 2014. Motivated by the identification of these viruses in domestic poultry in Canada, an intensive study was initiated to conduct highly pathogenic avian influenza surveillance in wild birds in the Pacific Flyway of the United States. A total of 4,729 hunter-harvested wild birds were sampled and highly pathogenic avian influenza virus was detected in 1.3% (n = 63). Three H5 clade 2.3.4.4 subtypes were isolated from wild birds, H5N2, H5N8, and H5N1, representing the wholly Eurasian lineage H5N8 and two novel reassortant viruses. Testing of 150 additional wild birds during avian morbidity and mortality investigations in Washington yielded 10 (6.7%) additional highly pathogenic avian influenza isolates (H5N8 = 3 and H5N2 = 7). The geographically widespread detection of these viruses in apparently healthy wild waterfowl suggest that the H5 clade 2.3.4.4 variant viruses may behave similarly in this taxonomic group whereby many waterfowl species are susceptible to infection but do not demonstrate obvious clinical disease. Despite these findings in wild waterfowl, mortality has been documented for some wild bird species and losses in US domestic poultry during the first half of 2015 were unprecedented. C1 [Bevins, S. N.; Gidlewski, T.; Baroch, J.; Grady, M. J.; Deliberto, T. J.] USDA, Natl Wildlife Res Ctr, Ft Collins, CO USA. [Dusek, R. J.; Bodenstein, B.; Ip, H. S.; Sleeman, J. M.] US Geol Survey, Natl Wildlife Hlth Ctr, Madison, WI USA. [Mansfield, K. G.; DeBruyn, P.; Kraege, D.; Rowan, E.] Washington Dept Fish & Wildlife, Olympia, WA USA. [Gillin, C.] Oregon Dept Fish & Wildlife, Corvallis, OR USA. [Thomas, B.] USDA Wildlife Serv, Salem, OR USA. [Chandler, S.] USDA Wildlife Serv, Sacramento, CA USA. [Miller, R. S.] USDA Vet Serv, Ft Collins, CO USA. [Drew, M. L.] Idaho Dept Fish & Game, Caldwell, ID USA. [Stopak, S.] USDA Wildlife Serv, Boise, ID USA. [Zscheile, B.] USDA Wildlife Serv, Salt Lake City, UT USA. [Bennett, J.; Sengl, J.] USDA Wildlife Serv, Reno, NV USA. [Brady, Caroline] Calif Waterfowl Assoc, Roseville, CA USA. [Spackman, E.] USDA ARS, Southeast Poultry Res Lab, Athens, GA USA. [Killian, M. L.; Torchetti, M. K.] USDA, Ames, IA USA. RP Bevins, SN (reprint author), USDA, Natl Wildlife Res Ctr, Ft Collins, CO USA. EM sarah.n.bevins@aphis.usda.gov OI Miller, Ryan/0000-0003-3892-0251; Dusek, Robert/0000-0001-6177-7479 NR 44 TC 9 Z9 9 U1 5 U2 9 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUL 6 PY 2016 VL 6 AR 28980 DI 10.1038/srep28980 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DQ4MG UT WOS:000379177400001 PM 27381241 ER PT J AU Ding, F Duan, YP Yuan, Q Shao, J Hartung, JS AF Ding, Fang Duan, Yongping Yuan, Qing Shao, Jonathan Hartung, John S. TI Serological detection of 'Candidatus Liberibacter asiaticus' in citrus, and identification by GeLC-MS/MS of a chaperone protein responding to cellular pathogens SO SCIENTIFIC REPORTS LA English DT Article ID REAL-TIME PCR; GREENING DISEASE; NITROCELLULOSE PAPER; TISSUE; PLANTS; HSP90; LOCALIZATION; VIRUS; HYBRIDIZATION; ANTIBODIES AB We describe experiments with antibodies against 'Candidatus Liberibacter asiaticus used to detect the pathogen in infected plants. We used scFv selected to bind epitopes exposed on the surface of the bacterium in tissue prints, with secondary monoclonal antibodies directed at a FLAG epitope included at the carboxyl end of the scFv. Unexpectedly, the anti-FLAG secondary antibody produced positive results with CaLas diseased samples when the primary scFv were not used. The anti-FLAG monoclonal antibody (Mab) also identified plants infected with other vascular pathogens. We then identified a paralogous group of secreted chaperone proteins in the HSP-90 family that contained the amino acid sequence DDDDK identical to the carboxy-terminal sequence of the FLAG epitope. A rabbit polyclonal antibody against one of the same epitopes combined with a goat anti-rabbit secondary antibody produced very strong purple color in individual phloem cells, as expected for this pathogen. These results were entirely specific for CaLas-infected citrus. The simplicity, cost and ability to scale the tissue print assay makes this an attractive assay to complement PCR-based assays currently in use. The partial FLAG epitope may itself be useful as a molecular marker for the rapid screening of citrus plants for the presence of vascular pathogens. C1 [Ding, Fang] Huazhong Agr Univ, Hubei Key Lab Plant Pathol, Wuhan 430070, Peoples R China. [Ding, Fang; Shao, Jonathan; Hartung, John S.] ARS, USDA, MPPL, 10300 Baltimore Ave, Beltsville, MD 20705 USA. [Duan, Yongping] ARS, USDA, USHRL, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. [Yuan, Qing] Sichuan Med Univ, Luzhou 646000, Sichuan, Peoples R China. RP Ding, F (reprint author), Huazhong Agr Univ, Hubei Key Lab Plant Pathol, Wuhan 430070, Peoples R China.; Ding, F; Hartung, JS (reprint author), ARS, USDA, MPPL, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM dinfany@mail.hzau.edu.cn; John.Hartung@ars.usda.gov FU Citrus Research and Development Foundation [551]; USDA-ARS FX This research was supported by grant 551 from the Citrus Research and Development Foundation and by funds from USDA-ARS. We thank Cristina Paul for skillful and tireless care of the diseased plants. NR 51 TC 0 Z9 0 U1 6 U2 6 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUL 6 PY 2016 VL 6 AR 29272 DI 10.1038/srep29272 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DQ4OZ UT WOS:000379184500001 PM 27381064 ER PT J AU Zhou, Y Utsunomiya, YT Xu, LY Hay, EA Bickhart, DM Sonstegard, TS Van Tassell, CP Garcia, JF Liu, GE AF Zhou, Yang Utsunomiya, Yuri T. Xu, Lingyang Hay, El Hamidi Abdel Bickhart, Derek M. Sonstegard, Tad S. Van Tassell, Curtis P. Garcia, Jose Fernando Liu, George E. TI Comparative analyses across cattle genders and breeds reveal the pitfalls caused by false positive and lineage-differential copy number variations SO SCIENTIFIC REPORTS LA English DT Article ID GENOME-WIDE DETECTION; HOLSTEIN CATTLE; SEGMENTAL DUPLICATIONS; STRUCTURAL VARIATION; ASSEMBLY ERRORS; BEADCHIP ARRAY; INTEGRATED MAP; SNP BEADCHIP; BLACK ANGUS; EVOLUTION AB We compared CNV region (CNVR) results derived from 1,682 Nellore cattle with equivalent results derived from our previous analysis of Bovine HapMap samples. By comparing CNV segment frequencies between different genders and groups, we identified 9 frequent, false positive CNVRs with a total length of 0.8 Mbp that were likely caused by assembly errors. Although there was a paucity of lineage specific events, we did find one 54 kb deletion on chr5 significantly enriched in Nellore cattle. A few highly frequent CNVRs present in both datasets were detected within genomic regions containing olfactory receptor, ATP-binding cassette, and major histocompatibility complex genes. We further evaluated their impacts on downstream bioinformatics and CNV association analyses. Our results revealed pitfalls caused by false positive and lineage-differential copy number variations and will increase the accuracy of future CNV studies in both taurine and indicine cattle. C1 [Zhou, Yang; Xu, Lingyang; Hay, El Hamidi Abdel; Bickhart, Derek M.; Sonstegard, Tad S.; Van Tassell, Curtis P.; Liu, George E.] ARS, Anim Genom & Improvement Lab, BARC, USDA, Beltsville, MD 20705 USA. [Zhou, Yang] Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Agr Mol Biol, Yangling 712100, Shaanxi, Peoples R China. [Utsunomiya, Yuri T.; Garcia, Jose Fernando] UNESP Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Med Vet Prevent & Reprod Anim, BR-14884900 Sao Paulo, Brazil. [Xu, Lingyang] Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China. [Garcia, Jose Fernando] UNESP Univ Estadual Paulista, Fac Med Vet Aracatuba, Dept Apoio Prod & Saude Anim, BR-16050680 Sao Paulo, Brazil. [Sonstegard, Tad S.] Recombinetics Inc, St Paul, MN 55104 USA. RP Liu, GE (reprint author), ARS, Anim Genom & Improvement Lab, BARC, USDA, Beltsville, MD 20705 USA.; Garcia, JF (reprint author), UNESP Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Med Vet Prevent & Reprod Anim, BR-14884900 Sao Paulo, Brazil.; Garcia, JF (reprint author), UNESP Univ Estadual Paulista, Fac Med Vet Aracatuba, Dept Apoio Prod & Saude Anim, BR-16050680 Sao Paulo, Brazil. EM jfgarcia@fmva.unesp.br; George.Liu@ars.usda.gov RI Utsunomiya, Yuri Tani/K-5214-2013; OI Utsunomiya, Yuri Tani/0000-0002-6526-8337; Bickhart, Derek/0000-0003-2223-9285 FU Agriculture and Food Research Initiative (AFRI) from the USDA National Institute of Food and Agriculture (NIFA) Animal Genome Program [2011-67015-30183] FX We also thank R. Anderson, and A. Dimtchev for technical assistance. This work was supported in part by Agriculture and Food Research Initiative (AFRI) competitive grant No. 2011-67015-30183 from the USDA National Institute of Food and Agriculture (NIFA) Animal Genome Program. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 48 TC 0 Z9 0 U1 1 U2 2 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUL 6 PY 2016 VL 6 AR 29219 DI 10.1038/srep29219 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DQ4OR UT WOS:000379183700001 PM 27381368 ER PT J AU Garcia, AM Alexander, RB Arnold, JG Norfleet, L White, MJ Robertson, DM Schwarz, G AF Garcia, Ana Maria Alexander, Richard B. Arnold, Jeffrey G. Norfleet, Lee White, Michael J. Robertson, Dale M. Schwarz, Gregory TI Regional Effects of Agricultural Conservation Practices on Nutrient Transport in the Upper Mississippi River Basin SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID WATER-QUALITY; SOUTHERN MINNESOTA; NITRATE SOURCES; SOIL-MOISTURE; DENITRIFICATION; PHOSPHORUS; CROPLAND; EMISSION; PROGRESS; INDIANA AB Despite progress in the implementation of conservation practices, related improvements in water quality have been challenging to measure in larger river systems. In this paper we quantify these downstream effects by applying the empirical U.S. Geological Survey water-quality model SPARROW to investigate whether spatial differences in conservation intensity were statistically correlated with variations in nutrient loads. In contrast to other forms of water quality data analysis, the application of SPARROW controls for confounding factors such as hydrologic variability, multiple sources and environmental processes. A measure of conservation intensity was derived from the USDA-CEAP regional assessment of the Upper Mississippi River and used as an explanatory variable in a model of the Upper Midwest. The spatial pattern of conservation intensity was negatively correlated (p = 0.003) with the total nitrogen loads in streams in the basin. Total phosphorus loads were weakly negatively correlated with conservation (p = 0.25). Regional nitrogen reductions were estimated to range from 5 to 34% and phosphorus reductions from 1 to 10% in major river basins of the Upper Mississippi region. The statistical associations between conservation and nutrient loads are consistent with hydrological and biogeochemical processes such as denitrification. The results provide empirical evidence at the regional scale that conservation practices have had a larger statistically detectable effect on nitrogen than on phosphorus loadings in streams and rivers of the Upper Mississippi Basin. C1 [Garcia, Ana Maria] US Geol Survey, 3916 Sunset Ridge Rd, Raleigh, NC USA. [Alexander, Richard B.; Schwarz, Gregory] US Geol Survey, Natl Ctr 432, Reston, VA 20192 USA. [Arnold, Jeffrey G.; White, Michael J.] ARS, USDA, Grassland Soil & Water Res Lab, 808 East Blackland Rd, Temple, TX 76502 USA. [Norfleet, Lee] USDA, Nat Resources & Conservat Serv, 101 East Blackland Rd, Temple, TX 76502 USA. [Robertson, Dale M.] US Geol Survey, 8505 Res Way, Middleton, WI 53562 USA. RP Garcia, AM (reprint author), US Geol Survey, 3916 Sunset Ridge Rd, Raleigh, NC USA. EM agarcia@usgs.gov OI Robertson, Dale/0000-0001-6799-0596 FU U.S. Geological Survey National Water Quality Program; U.S. Department of Agriculture Conservation Effects Assessment Program FX This research was funded by the U.S. Geological Survey National Water Quality Program and the U.S. Department of Agriculture Conservation Effects Assessment Program. NR 43 TC 1 Z9 1 U1 13 U2 20 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUL 5 PY 2016 VL 50 IS 13 SI SI BP 6991 EP 7000 DI 10.1021/acs.est.5b03543 PG 10 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DQ7DE UT WOS:000379366300048 PM 27243625 ER PT J AU Stonis, JR Diskus, A Remeikis, A Davis, DR Solis, MA Torres, NC AF Stonis, Jonas R. Diskus, Arunas Remeikis, Andrius Davis, Donald R. Solis, M. Alma Cumbicus Torres, Nixon TI The first record of Baccharis L. (Asteraceae) as a host-plant genus for Nepticulidae (Lepidoptera), with description of new Stigmella species from South America SO ZOOTAXA LA English DT Article DE Baccharis L.; host-plants; leaf-mines; Nepticulidae; new species; South America; Stigmella ID LEAF-MINING NEPTICULIDAE; COMPOSITAE AB We record the first Nepticulidae species found to feed on Baccharis L. ( Asteraceae). Despite the high species richness of Baccharis in the Western Hemisphere, no nepticulid has ever been recorded feeding on Baccharis. In this paper we describe six new Stigmella Schrank species feeding on Baccharis: S. emarginatae Diskus & Stonis, sp. nov., S. bipartita Diskus & Stonis, sp. nov., S. tripartita Diskus & Stonis, sp. nov., S. latifoliae Remeikis, Diskus & Stonis, sp. nov., S. baccharicola Diskus & Stonis, sp. nov., and S. confertae Diskus & Stonis, sp. nov. The remaining two taxa are left unnamed. All taxa are illustrated with photographs of adults, their genitalia, and their leaf-mines. Additionally, leaf- mines on Baccharis salicifolia are documented. C1 [Stonis, Jonas R.; Diskus, Arunas; Remeikis, Andrius] Lithuanian Univ Educ Sci, Studentu St 39, LT-08106 Vilnius, Lithuania. [Stonis, Jonas R.; Diskus, Arunas; Remeikis, Andrius] Baltic Amer Biotaxon Inst, Studentu St 39, LT-08106 Vilnius, Lithuania. [Remeikis, Andrius] Nat Res Ctr, Akad St 2, LT-08412 Vilnius, Lithuania. [Davis, Donald R.] Smithsonian Inst, Natl Museum Nat Hist, Washington, DC 20013 USA. [Davis, Donald R.; Solis, M. Alma] Baltic Amer Biotaxon Inst, Washington, DC 20013 USA. [Solis, M. Alma] ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, Washington, DC 20013 USA. [Cumbicus Torres, Nixon] Univ Tecn Particular Loja, San Cayetano Alto S-N, Loja, Ecuador. RP Stonis, JR (reprint author), Lithuanian Univ Educ Sci, Studentu St 39, LT-08106 Vilnius, Lithuania.; Stonis, JR (reprint author), Baltic Amer Biotaxon Inst, Studentu St 39, LT-08106 Vilnius, Lithuania. EM stonis@leu.lt FU Research Foundation of the Research Council of Lithuania; Baltic-American Biotaxonomy Institute; Research Foundation of the Lithuanian University of Educational Sciences FX We are indebted to O. Karsholt and late Professor N. P. Kristensen (ZMUC) for the initial stimulus for the Neotropical project together with generous support during its course and the loan of Neotropical material. We are also grateful to our colleague Dr. Dimitri Forero (Laboratorio de Entomologia, UNESIS, Departamento de Biologia, Pontificia Universidad Javeriana, Bogota, Colombia). Dr. Arunas Diskus is thankful to Modestas Jocius for his kind assistance in investigating the Nepticulidae fauna of the Andes and to the Research Foundation of the Research Council of Lithuania for the research stripend in 2016. Andrius Remeikis is grateful to the Baltic-American Biotaxonomy Institute for the research stipend in 2015. This study was supported from the Research Foundation of the Lithuanian University of Educational Sciences (2015). NR 23 TC 4 Z9 4 U1 5 U2 6 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD JUL 5 PY 2016 VL 4136 IS 1 BP 101 EP 128 DI 10.11646/zootaxa.4136.1.4 PG 28 WC Zoology SC Zoology GA DQ5AT UT WOS:000379217500004 PM 27395706 ER PT J AU Song, J Li, SX Xu, YL Wei, W Yao, Q Pan, FJ AF Song, Jie Li, Shuxian Xu, Yanli Wei, Wei Yao, Qin Pan, Fengjuan TI Diversity of parasitic fungi from soybean cyst nematode associated with long-term continuous cropping of soybean in black soil SO ACTA AGRICULTURAE SCANDINAVICA SECTION B-SOIL AND PLANT SCIENCE LA English DT Article DE Biodiversity; community; Heterodera glycines; parasitic fungal; suppressive soil ID REAL-TIME PCR; HETERODERA-GLYCINES; HIRSUTELLA-RHOSSILIENSIS; POCHONIA-CHLAMYDOSPORIA; 2ND-STAGE JUVENILES; PASTEURIA SP; BACTERIAL; SEQUENCE; TILLAGE; FIELDS AB The soybean cyst nematode (SCN) is a major yield-limiting pest of soybean. In this study, experiments were conducted to examine the diversity of parasitic fungi from SCN associated with disease-suppressive soil fields in Northeast China. Soil samples were collected from three fields under different rotation systems that were established in 1991: (1) a continuous long-term cropping field with soybean (SSSS) that had been shown to be SCN-suppressive, (2) cycles of three-year rotation with corn, soybean, and wheat (WCS), and (3) continuous cropping field with three-year cycles of two years soybean and one-year corn (SSC). In the traditional method result, cyst densities of SCN declined as increase of parasitic fungi, and the percentage of parasitic fungi associated with cyst of SCN was higher in SSSS field than other two fields. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) also showed that parasitic fungi of SCN were also increased in SSSS field, compared with the other two fields. Principal component analysis based on PCR-DGGE data revealed that fungal communities on cysts could be divided into three groups: one group occurred in SSSS, and the other two groups were in WCS and SSC fields, respectively. Long-term cropping with soybean monoculture in the black soil field might increase parasitic fungi of SCN. These fungal communities may play an important role in the ecological suppression of SCN in disease-suppressive soil. C1 [Song, Jie; Xu, Yanli] Northeast Agr Univ, Coll Agr, Harbin 150030, Peoples R China. [Song, Jie; Xu, Yanli; Wei, Wei; Yao, Qin; Pan, Fengjuan] Chinese Acad Sci, Northeast Inst Geog & Agroecol, Harbin 150081, Peoples R China. [Li, Shuxian] USDA ARS, Crop Genet Res Unit, 141 Expt Stn Rd, Stoneville, MS 38776 USA. RP Xu, YL (reprint author), Northeast Agr Univ, Coll Agr, Harbin 150030, Peoples R China.; Xu, YL (reprint author), Chinese Acad Sci, Northeast Inst Geog & Agroecol, Harbin 150081, Peoples R China.; Li, SX (reprint author), USDA ARS, Crop Genet Res Unit, 141 Expt Stn Rd, Stoneville, MS 38776 USA. EM shuxian.li@ars.usda.gov; xyll@iga.ac.cn FU National Natural Science Foundation of China [41571253] FX This work was supported by the National Natural Science Foundation of China [grant number 41571253]. NR 52 TC 0 Z9 1 U1 18 U2 49 PU TAYLOR & FRANCIS AS PI OSLO PA KARL JOHANS GATE 5, NO-0154 OSLO, NORWAY SN 0906-4710 EI 1651-1913 J9 ACTA AGR SCAND B-S P JI Acta Agric. Scand. Sect. B-Soil Plant Sci. PD JUL 3 PY 2016 VL 66 IS 5 BP 432 EP 442 DI 10.1080/09064710.2016.1158862 PG 11 WC Agronomy; Soil Science SC Agriculture GA DJ1HR UT WOS:000373954400008 ER PT J AU Marzano, SYL Domier, LL AF Marzano, Shin-Yi Lee Domier, Leslie L. TI Reprint of "Novel mycoviruses discovered from metatranscriptomics survey of soybean phyllosphere phytobiomes" SO VIRUS RESEARCH LA English DT Article; Proceedings Paper CT 3rd International Mycovirus Symposium (IMS) CY AUG 02-04, 2014 CL Univ Vermont, Burlington, VT SP Univ Rutgers HO Univ Vermont DE High-throughput sequencing metatranscriptome; Phyllosphere; Soybean; Viromes ID DOUBLE-STRANDED-RNA; MOLECULAR CHARACTERIZATION; SCLEROTINIA-SCLEROTIORUM; BIOLOGICAL-CONTROL; CHESTNUT BLIGHT; FUNGAL VIRUSES; DNA MYCOVIRUS; PLANT; ALIGNMENT; GENOME AB Mycoviruses can be beneficial to plants in that they can debilitate pathogenic fungi thereby reducing the severity of associated plant diseases. Studies to date have focused primarily on culturable fungi that represent a fraction of natural fungal populations. The nonculturable fungi, however, can harbor diverse populations of mycoviruses that reduce plant disease or enhance resistance to abiotic stress. Metatranscriptome analysis of field-grown plant samples using high-throughput sequencing offers the possibility of unbiased detection and quantification of mycoviruses regardless of the culturability of their fungal hosts together with the complete associated microbial consortia. In this study, we describe the fungal viromes of the phyllosphere of production soybean fields in Illinois, USA by analyzing the metatranscriptomes of thousands of soybean leaf samples collected during the 2008 and 2009 growing seasons. The analyses identified 25 partial genome sequences that represented at least 22 mycovirus genomes, only one of which had been described previously. The novel mycovirus genomes showed similarity to 10 distinct lineages including the genera Alphapartitivirus, Botybirnavirus, Endornavirus, Mitovirus, Mycoflexivirus, Ourmiavirus, Totivirus, Victorivirus, family Tombusviridae, order Mononegavirales, and the recently proposed genus Gemycircularvirus. The present study adds to the wealth of mycoviruses associated with plant phytobiomes and establishes groundwork needed for further characterization of the viruses. Published by Elsevier B.V. C1 [Marzano, Shin-Yi Lee; Domier, Leslie L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Domier, Leslie L.] USDA ARS, Urbana, IL USA. [Marzano, Shin-Yi Lee] S Dakota State Univ, Dept Plant Sci, Dept Biol & Microbiol, Brookings, SD 57007 USA. RP Domier, LL (reprint author), Univ Illinois, Dept Crop Sci, USDA ARS, Urbana, IL 61801 USA. EM leslie.domier@ars.usda.gov NR 71 TC 2 Z9 2 U1 4 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 EI 1872-7492 J9 VIRUS RES JI Virus Res. PD JUL 2 PY 2016 VL 219 SI SI BP 11 EP 21 DI 10.1016/j.virusres.2016.05.012 PG 11 WC Virology SC Virology GA DQ1JV UT WOS:000378958200003 PM 27208848 ER PT J AU Campbell, BT Greene, J Wu, J Jones, DC AF Campbell, B. T. Greene, J. Wu, J. Jones, D. C. TI Genetic Variation for Agronomic and Fiber Quality Traits in a Population Derived from High-Quality Cotton Germplasm SO CROP SCIENCE LA English DT Article ID UPLAND COTTON; BUNDLE STRENGTH; GENOME SEQUENCE; REGISTRATION; LINES; IMPROVEMENT; COMPONENTS; EVOLUTION; NUMBER; YIELD AB Genetic improvement of fiber quality is necessary to meet the requirements of processors and users of upland cotton (Gossypium hirsutum L.) fiber. To foster genetic improvement of cotton fiber quality, adequate genetic variation for the quantitatively inherited physical properties of cotton is required. Additionally, knowledge of the genetic architecture of fiber quality is needed to design effective breeding strategies to further improve fiber quality. In this study, our objective was to estimate genetic variance components and predict genetic effects for agronomic and fiber quality traits in a population derived from four known genotypic sources of high fiber quality. The majority of genetic variation present in a half-diallel population derived from these four sources of high fiber quality was due to additive effects. Predicted genetic effects demonstrated that one of the four parents, MD 15, provides a unique genetic source of high fiber quality alleles that behave additively. C1 [Campbell, B. T.] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, 2611 W Lucas St, Florence, SC 29501 USA. [Greene, J.] Clemson Univ, Edisto Res & Educ Ctr, 64 Res Rd, Blackville, SC 29817 USA. [Wu, J.] South Dakota State Univ, Dept Plant Sci, Box 2140C, Brookings, SD 57007 USA. [Jones, D. C.] Cotton Inc, 6399 Weston Pkwy, Cary, NC USA. RP Campbell, BT (reprint author), USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, 2611 W Lucas St, Florence, SC 29501 USA. EM todd.campbell@ars.usda.gov FU CRIS of the USDA [6657-21000-006-00D]; USDA-National Institute of Food and Agriculture Hatch Project [1005459]; Cotton Incorporated Project [07-157] FX This research project was supported by funding from CRIS No. 6657-21000-006-00D of the USDA (BTC), USDA-National Institute of Food and Agriculture Hatch Project No. 1005459 (JW), and Cotton Incorporated Project 07-157 (BTC). Special thanks to Bobby Fisher and summer students for technical assistance. Special thanks also to Dr. William R. Meredith, Jr. (Deceased Research Geneticist, USDA-ARS) for providing seed of MD 15. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. NR 41 TC 0 Z9 0 U1 1 U2 1 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 1689 EP 1697 DI 10.2135/cropsci2015.10.0657 PG 9 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700025 ER PT J AU Skinner, RH Dell, CJ AF Skinner, R. Howard Dell, Curtis J. TI Yield and Soil Carbon Sequestration in Grazed Pastures Sown with Two or Five Forage Species SO CROP SCIENCE LA English DT Article ID NORTHEAST UNITED-STATES; NUTRITIVE-VALUE; ORGANIC-MATTER; ROOT-GROWTH; GRASSLAND; DIVERSITY; MANAGEMENT; MIXTURES; PRODUCTIVITY; BUDGET C1 [Skinner, R. Howard; Dell, Curtis J.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. RP Skinner, RH (reprint author), USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. EM howard.skinner@ars.usda.gov NR 40 TC 2 Z9 2 U1 0 U2 0 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 2035 EP 2044 DI 10.2135/cropsci2015.11.0711 PG 10 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700057 ER PT J AU Skinner, RH Goslee, SC AF Skinner, R. Howard Goslee, Sarah C. TI Defoliation Effects on Pasture Photosynthesis and Respiration SO CROP SCIENCE LA English DT Article ID CARBON-DIOXIDE EXCHANGE; SOUTHERN PIEDMONT USA; SOIL ORGANIC C; TEMPERATE GRASSLAND; MANAGEMENT; FLUXES; PLANT; CO2; NITROGEN; DUNG AB Ecosystem C gain or loss from managed grasslands depends on management practices. However, limited information is available at the field scale on how the type of defoliation, specifically grazing vs. cutting, affects gross primary productivity (GPP) and ecosystem respiration (R-E) immediately after defoliation and during the regrowth process. This study takes advantage of daily field-scale micrometeorological measurements of GPP and R-E made over a 9-yr period on two pastures that were grazed or cut approximately five times per year to examine the relative effects of grazing and cutting on GPP and R-E. The null hypotheses were that the initial reduction in GPP and R-E following defoliation and the time required for recovery to pre-defoliation levels did not differ in response to defoliation method. Overall, R-E was greater in cut pastures than in grazed pastures. Regardless of defoliation method, R-E did not change during the first week after defoliation, or during the 5 wk following. The initial reduction in GPP(2000) (GPP in full sunlight) was greater when pastures were cut (70%) than grazed (46%) and recovery to pre-defoliation levels took 5 wk for cut pastures vs. 3 wk when grazed. This study suggests, all other management practices being similar, that grazed pastures should be stronger annual C sinks than cut pastures. However, cut and grazed pastures are rarely managed in the same way such that the realized balance between GPP and R-E will depend on both physiological responses to defoliation and management intensity. C1 [Skinner, R. Howard; Goslee, Sarah C.] USDA ARS, Pasture Syst & Watershed Management Res Unit, Bldg 3702 Curtin Rd, University Pk, PA 16802 USA. RP Skinner, RH (reprint author), USDA ARS, Pasture Syst & Watershed Management Res Unit, Bldg 3702 Curtin Rd, University Pk, PA 16802 USA. EM howard.skinner@ars.usda.gov NR 40 TC 0 Z9 0 U1 1 U2 1 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 2045 EP 2053 DI 10.2135/cropsci2015.12.0733 PG 9 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700058 ER PT J AU Cunha, CW Taus, NS Dewals, BG Vanderplasschen, A Knowles, DP Li, H AF Cunha, Cristina W. Taus, Naomi S. Dewals, Benjamin G. Vanderplasschen, Alain Knowles, Donald P. Li, Hong TI Replacement of Glycoprotein B in Alcelaphine Herpesvirus 1 by Its Ovine Herpesvirus 2 Homolog : Implications in Vaccine Development for Sheep-Associated Malignant Catarrhal Fever SO MSPHERE LA English DT Article DE Chimeric virus; alcelaphine herpesvirus 1; malignant catarrhal fever; ovine herpesvirus 2; vaccine ID ANTIBODY; CATTLE; VIRUS; INFECTIVITY; RABBITS; ENTRY; ASSAY; GB AB Vaccine development is a top priority in malignant catarrhal fever (MCF) research. In the case of sheep-associated MCF (SA-MCF) caused by ovine herpesvirus 2 (OvHV-2), progress toward this objective has been hindered by the absence of methods to attenuate or modify the virus, since it cannot be propagated in vitro. As an alternative for vaccine development, in this study, we tested the hypothesis that one of the SA-MCF vaccine candidate targets, OvHV-2 glycoprotein B (gB), could be expressed by a nonpathogenic alcelaphine herpesvirus 1 (AlHV-1) and then evaluated the potential of the AlHV-1/OvHV-2 chimera to be used as a vaccine and a diagnostic tool. The construction and characterization of an AlHV-1/OvHV-2 chimeric virus that is nonpathogenic and expresses an OvHV-2 vaccine target are significant steps toward the development of an SA-MCF vaccine and also provide a valuable means to study OvHV-2 biology. C1 [Cunha, Cristina W.; Taus, Naomi S.; Knowles, Donald P.; Li, Hong] ARS, Anim Dis Res Unit, USDA, Pullman, WA 99164 USA. [Cunha, Cristina W.; Taus, Naomi S.; Knowles, Donald P.; Li, Hong] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Dewals, Benjamin G.; Vanderplasschen, Alain] Univ Liege, Fac Vet Med, Immunol Vaccinol, FARAH, Liege, Belgium. EM cwcunha@vetmed.wsu.edu FU U.S. Department of Agriculture (USDA) [CWU 2090-32000-032-00D] FX This work, including the efforts of Cristina W. Cunha, Naomi S. Taus, Donald P. Knowles, and Hong Li, was funded by U.S. Department of Agriculture (USDA) (CWU 2090-32000-032-00D). NR 21 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 2379-5042 J9 MSPHERE JI mSphere PD JUL-AUG PY 2016 VL 1 IS 4 AR e00108-16 DI 10.1128/mSphere.00108-16 PG 11 WC Microbiology SC Microbiology GA EI6DU UT WOS:000392586000005 ER PT J AU Lorch, JM Palmer, JM Lindner, DL Ballmann, AE George, KG Griffin, K Knowles, S Huckabee, JR Haman, KH Anderson, CD Becker, PA Buchanan, JB Foster, JT Blehert, DS AF Lorch, Jeffrey M. Palmer, Jonathan M. Lindner, Daniel L. Ballmann, Anne E. George, Kyle G. Griffin, Kathryn Knowles, Susan Huckabee, John R. Haman, Katherine H. Anderson, Christopher D. Becker, Penny A. Buchanan, Joseph B. Foster, Jeffrey T. Blehert, David S. TI First Detection of Bat White-Nose Syndrome in Western North America SO MSPHERE LA English DT Article DE Pseudogymnoascus destructans; Washington; bat; white-nose syndrome ID GEOMYCES-DESTRUCTANS; SPREAD AB White-nose syndrome (WNS) is an emerging fungal disease of bats caused by Pseudogymnoascus destructans. Since it was first detected near Albany, NY, in 2006, the fungus has spread across eastern North America, killing unprecedented numbers of hibernating bats. The devastating impacts of WNS on Nearctic bat species are attributed to the likely introduction of P. destructans from Eurasia to naive host populations in eastern North America. Since 2006, the disease has spread in a gradual wavelike pattern consistent with introduction of the pathogen at a single location. Here, we describe the first detection of P. destructans in western North America in a little brown bat (Myotis lucifugus) from near Seattle, WA, far from the previously recognized geographic distribution of the fungus. Whole-genome sequencing and phylogenetic analyses indicated that the isolate of P. destructans from Washington grouped with other isolates of a presumed clonal lineage from the eastern United States. Thus, the occurrence of P. destructans in Washington does not likely represent a novel introduction of the fungus from Eurasia, and the lack of intensive surveillance in the western United States makes it difficult to interpret whether the occurrence of P. destructans in the Pacific Northwest is disjunct from that in eastern North America. Although there is uncertainty surrounding the impacts of WNS in the Pacific Northwest, the presence of the pathogen in western North America could have major consequences for bat conservation. IMPORTANCE White-nose syndrome (WNS) represents one of the most consequential wildlife diseases of modern times. Since it was first documented in New York in 2006, the disease has killed millions of bats and threatens several formerly abundant species with extirpation or extinction. The spread of WNS in eastern North America has been relatively gradual, inducing optimism that disease mitigation strategies could be established in time to conserve bats susceptible to WNS in western North America. The recent detection of the fungus that causes WNS in the Pacific Northwest, far from its previous known distribution, increases the urgency for understanding the long-term impacts of this disease and for developing strategies to conserve imperiled bat species. C1 [Lorch, Jeffrey M.; Ballmann, Anne E.; George, Kyle G.; Griffin, Kathryn; Knowles, Susan; Blehert, David S.] US Geol Survey, Natl Wildlife Hlth Ctr, Madison, WI 53711 USA. [Palmer, Jonathan M.; Lindner, Daniel L.] US Forest Serv, Northern Res Stn, Ctr Forest Mycol Res, Madison, WI USA. [Huckabee, John R.] PAWS Wildlife Ctr, Lynnwood, WA USA. [Haman, Katherine H.; Anderson, Christopher D.; Becker, Penny A.; Buchanan, Joseph B.] Washington Dept Fish & Wildlife, Olympia, WA USA. [Foster, Jeffrey T.] Univ New Hampshire, Dept Mol Cellular & Biomed Sci, Durham, NH 03824 USA. EM jlorch@usgs.gov OI Anderson, Christopher/0000-0002-0053-2002; Palmer, Jonathan/0000-0003-0929-3658 FU DOI \ U.S. Geological Survey (USGS); USDA \ U.S. Forest Service (USFS) FX This work, including the efforts of Jeffrey M. Lorch, was funded by DOI vertical bar U.S. Geological Survey (USGS). This work, including the efforts of Daniel L. Lindner, was funded by USDA vertical bar U.S. Forest Service (USFS). NR 24 TC 1 Z9 1 U1 9 U2 9 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 2379-5042 J9 MSPHERE JI mSphere PD JUL-AUG PY 2016 VL 1 IS 4 AR e00148-16 DI 10.1128/mSphere.00148-16 PG 5 WC Microbiology SC Microbiology GA EI6DU UT WOS:000392586000011 ER PT J AU Cela, S Ketterings, QM Czymmek, KJ Weld, J Beegle, DB Kleinman, PJA AF Cela, S. Ketterings, Q. M. Czymmek, K. J. Weld, J. Beegle, D. B. Kleinman, P. J. A. TI Nutrient management planners' feedback on New York and Pennsylvania phosphorus indices SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE best management practices; manure; nutrient management planners; phosphorus index; survey ID NEED AB State phosphorus indices (PIs) are being evaluated across the United States due to variability in phosphorus (P) management recommendations and questions about the lack of water quality improvement in some watersheds. Nutrient management planners in New York (NY) and Pennsylvania (PA) were surveyed via two separate "but related" questionnaires to document perspectives on the current NY-PI and PA-PI and to obtain recommendations for improvements. Many planners were content with the current versions of the PIs but felt improvements could be made to more strongly discourage application of manure under conditions of high P loss potential and better promote certain best management practices. The NY planners felt that the NY-PI should discourage manure application during winter and to fields near streams, and should more strongly promote manure incorporation or injection, establishment of cover crops, ground coverage with crop residues, and implementation of setbacks and vegetated buffers. Similarly, the PA planners felt that the PA-PI should more strongly discourage manure application to fields with insufficient ground cover, near subsurface drainage and surface inlets, and during winter. In addition, the PA planners said the PA-PI should more strongly encourage soil conservation practices such as no-till, use of cover crops, and vegetated buffers. Results of the survey suggest common experiences and viewpoints among planners in NY and PA, resulting in a valuable on-the-ground assessment of the PIs as a nutrient management planning tool in both states, and the potential for development of a single, physiographic region PI. C1 [Cela, S.; Czymmek, K. J.] Cornell Univ, Dept Anim Sci, PRO DAIRY, Ithaca, NY 14853 USA. [Ketterings, Q. M.] Cornell Univ, Dept Anim Sci, PRO DAIRY, Nutrient Management Agr Syst, Ithaca, NY 14853 USA. [Weld, J.] Penn State Univ, Ecosyst & Sci Management Dept, University Pk, PA 16802 USA. [Beegle, D. B.] Penn State Univ, Dept Plant Sci, Agron, University Pk, PA 16802 USA. [Kleinman, P. J. A.] USDA ARS, University Pk, PA USA. RP Cela, S (reprint author), Cornell Univ, Dept Anim Sci, PRO DAIRY, Ithaca, NY 14853 USA. FU USDA Conservation Innovation Grant [4782-CU-USDA-2226] FX We thank all the nutrient management planners in New York and Pennsylvania for replying to the surveys. This work was funded by a USDA Conservation Innovation Grant (4782-CU-USDA-2226). NR 11 TC 0 Z9 0 U1 0 U2 0 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD JUL-AUG PY 2016 VL 71 IS 4 BP 281 EP 288 DI 10.2489/jswc.71.4.281 PG 8 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA EH7KF UT WOS:000391951000004 ER PT J AU Wang, J Watts, DB Meng, Q Zhang, Q Wu, F Torbert, HA AF Wang, J. Watts, D. B. Meng, Q. Zhang, Q. Wu, F. Torbert, H. A. TI Soil water infiltration impacted by maize (Zea mays L.) growth on sloping agricultural land of the Loess Plateau SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE Green-Ampt model; maize growth stage; rainfall infiltration; rainfall simulation; sloping land ID RAINFALL; CHINA; INTERCEPTION; VARIABILITY; ECOSYSTEMS; MOISTURE; EROSION; VEGETATION; TILLAGE; CANOPY AB Increasing infiltration rates of sloping agricultural land from arid and semiarid regions not only affects water supply and precipitation transformations in soil directly, but also impacts erosion intensity. This is extremely important to the Loess Plateau regions of northwest China, where a majority of the rainfall occurs within three months of the year, and soils left bare during these months are considered the norm. Incorporating an actively growing crop during this period could protect the soil surface and increase water infiltration. Thus, the objective of this study was to evaluate the combined effects of landscape gradients and maize (Zea mays L.) growth stage on soil water infiltration from agricultural land using runoff plots with slopes ranging from 5.24% to 28.78% conducted under rainfall simulation based on the water balance method. A modified Green-Ampt model was also used under the artificial rainfall conditions to simulate infiltration at different maize growth stages. Results showed that maize plants can intercept precipitation and delay surface water runoff. The capacity of interception increased greatly with maize growth from seedling to the reproductive stage. Steady state infiltration rate improved significantly; cumulative infiltration before runoff and cumulative infiltration from the artificial precipitation were higher under an actively growing maize crop than bare ground. Infiltration rates decreased with increasing slope. Regardless of slope, this study showed that rainfall infiltration rates were enhanced in the presence of actively growing plants compared to bare soil and improved as the growth progressed over the season. The Green-Ampt model has been proposed as a tool to provide improved soil infiltration predictions, which are in theory supposed to be closely related to measured values. A good correlation was observed between the measured and simulated values. However, the predicted values were slightly overestimated using the Green-Ampt equations, which may be attributed to the model not taking into consideration soil compaction from raindrops hitting the soil surface. C1 [Wang, J.; Zhang, Q.; Wu, F.] Northwest A&F Univ, Coll Nat Resources & Environm, Yangling, Shaanxi, Peoples R China. [Meng, Q.] Northwest A&F Univ, Coll Water Resources & Architectural Engn, Yangling, Shaanxi, Peoples R China. [Watts, D. B.; Torbert, H. A.] USDA ARS, Natl Soil Dynam Lab, Auburn, AL USA. RP Wang, J (reprint author), Northwest A&F Univ, Coll Nat Resources & Environm, Yangling, Shaanxi, Peoples R China. NR 49 TC 0 Z9 0 U1 5 U2 5 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD JUL-AUG PY 2016 VL 71 IS 4 BP 301 EP 309 DI 10.2489/jswc.71.4.301 PG 9 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA EH7KF UT WOS:000391951000006 ER PT J AU Vigdorovich, V Oliver, BG Carbonetti, S Dambrauskas, N Lange, MD Yacoob, C Leahy, W Callahan, J Stamatatos, L Sather, DN AF Vigdorovich, Vladimir Oliver, Brian G. Carbonetti, Sara Dambrauskas, Nicholas Lange, Miles D. Yacoob, Christina Leahy, Will Callahan, Jonathan Stamatatos, Leonidas Sather, D. Noah TI Repertoire comparison of the B-cell receptor-encoding loci in humans and rhesus macaques by next-generation sequencing SO CLINICAL & TRANSLATIONAL IMMUNOLOGY LA English DT Article ID SIMIAN IMMUNODEFICIENCY VIRUS; NONHUMAN PRIMATE MODELS; NEUTRALIZING ANTIBODIES; SINGLE-CELL; HIV-1 GP120; INFECTION; BROAD; IMMUNIZATION; AIDS; VACCINE AB Rhesus macaques (RMs) are a widely used model system for the study of vaccines, infectious diseases and microbial pathogenesis. Their value as a model lies in their close evolutionary relationship to humans, which, in theory, allows them to serve as a close approximation of the human immune system. However, despite their prominence as a human surrogate model system, many aspects of the RM immune system remain ill characterized. In particular, B cell-mediated immunity in macaques has not been sufficiently characterized, and the B-cell receptor-encoding loci have not been thoroughly annotated. To address these gaps, we analyzed the circulating heavy-and light-chain repertoires in humans and RMs by next-generation sequencing. By comparing V gene segment usage, J-segment usage and CDR3 lengths between the two species, we identified several important similarities and differences. These differences were especially notable in the IgM(+) B-cell repertoire. However, the class-switched, antigen-educated B-cell populations converged on a set of similar characteristics, implying similarities in how each species responds to antigen. Our study provides the first comprehensive overview of the circulating repertoires of the heavy-and light-chain sequences in RMs, and provides insight into how they may perform as a model system for B cell-mediated immunity in humans. C1 [Vigdorovich, Vladimir; Oliver, Brian G.; Carbonetti, Sara; Dambrauskas, Nicholas; Lange, Miles D.; Sather, D. Noah] Ctr Infect Dis Res, Seattle, WA 98109 USA. [Yacoob, Christina; Stamatatos, Leonidas] Fred Hutchinson Canc Res Ctr, Viral & Infect Dis Div, 1124 Columbia St, Seattle, WA 98104 USA. [Leahy, Will; Callahan, Jonathan] Mazama Sci, Seattle, WA USA. [Lange, Miles D.] USDA ARS, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR USA. RP Sather, DN (reprint author), Ctr Infect Dis Res, Seattle, WA 98109 USA. EM noah.sather@CIDResearch.org FU NIH/NIAID [R33 AI089405, R01 AI104384] FX We gratefully acknowledge the veterinary staff at the Washington National Primate Research Center, Seattle WA, USA, including J Ogle and M Gough. We thank Dr F Matsen IV for his insightful critique of this manuscript. This study was funded by NIH/NIAID R33 AI089405 to DNS and R01 AI104384 to LS. NR 70 TC 1 Z9 1 U1 0 U2 1 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2050-0068 J9 CLIN TRANSL IMMUNOL JI Clin. Transl. Immunol. PD JUL PY 2016 VL 5 AR e93 DI 10.1038/cti.2016.42 PG 11 WC Immunology SC Immunology GA EG4GQ UT WOS:000391002200006 PM 27525066 ER PT J AU Lintz, HE Gray, AN Yost, A Sniezko, R Woodall, C Reilly, M Hutten, K Elliott, M AF Lintz, H. E. Gray, A. N. Yost, A. Sniezko, R. Woodall, C. Reilly, M. Hutten, K. Elliott, M. TI Quantifying density-independent mortality of temperate tree species SO ECOLOGICAL INDICATORS LA English DT Article DE Tree; Population; Indicator; Species; Mortality ID WESTERN UNITED-STATES; CLIMATE-CHANGE; CHANGING CLIMATE; TURNOVER RATES; GLOBAL-CHANGE; BLISTER RUST; FOREST; RESPONSES; BIODIVERSITY; NORTHWEST AB Forest resilience to climate change is a topic of national concern as our standing assets and future forests are important to our livelihood. Many tree species are predicted to decline' or disappear while others may be able to adapt or migrate. Efforts to quantify and disseminate the current condition of forests are urgently needed to guide management and policy. Here, we develop a new indicator to summarize raw density-independent mortality of forested stands by species from the last decade of the 20th century to the first decade of the 21st century using forest inventory data. We define density-independent mortality to be stand mortality by species due to processes unrelated to natural mortality from succession or stand maturation, which is marked by overall increase in tree girth at the expense of density of individuals. We assess trends for 22 species on national forests in two U.S. states, Washington and Oregon. Populations of some species including timber species have no or low overall levels of density-independent mortality (Juniperus occidentalis, Abies procera, Thuja plicata, Tsuga heterophylla, Pinus ponderosa, and Pseudotsuga menziesii). In contrast, other species demonstrate unsustainable levels of density-independent mortality (Pinus monticola, Arbutus menziesii, Pinus albicaulis, Abies lasiocarpa, Taxus brevifolia, Pinus contorta, Abies grandis, Picea englemanii, and Larix occidentalis). Additionally, the net potential for unsustainable levels of density-independent mortality in standing populations does not necessarily warrant concern when examined across species for our study area and time period; however, when examined by species, the number of species in decline exceeds the number of species where mortality can be generally attributed to endogenous self-thinning. We argue that this work can aid management and policy decisions and our understanding of complex vegetation dynamics in a changing climate. Application of the indicator at larger spatial scales and in conjunction with data on migration and establishment may be used to address questions such as, how can we make cost-effective management decisions to ensure long-term sustainability of tree species and forests? Tree species distributions across the landscape are complex systems, and raw characterization of current trends occurring in forest inventories is important especially given the uncertainty associated with the modeling and prediction of complex systems such as tree species. (C) 2016 Published by Elsevier Ltd. C1 [Lintz, H. E.] Oregon State Univ, Coll Earth Ocean & Atmospher Sci, Oregon Climate Change Res Inst, Corvallis, OR 97330 USA. [Gray, A. N.] USDA Forest Serv, PNW Res Stn, Corvallis, OR USA. [Yost, A.] Oregon Dept Forestry, Salem, OR USA. [Sniezko, R.] USDA Forest Serv, Dorena Genet Resource Ctr, Cottage Grove, OR USA. [Woodall, C.] USDA Forest Serv, Northern Res Stn, St Paul, MN USA. [Reilly, M.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Hutten, K.] Univ Washington, Dept Forest Ecol, Seattle, WA 98195 USA. [Elliott, M.] Washington State Univ, Puyallup Res & Extens Ctr, Puyallup, WA 98371 USA. RP Lintz, HE (reprint author), Oregon State Univ, Coll Earth Ocean & Atmospher Sci, Oregon Climate Change Res Inst, Corvallis, OR 97330 USA. EM hlintz@coas.oregonstate.edu FU Oregon Department of Forestry; US Forest Service FX We thank Oregon Department of Forestry and the US Forest Service for funding this work. NR 73 TC 0 Z9 0 U1 4 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1470-160X EI 1872-7034 J9 ECOL INDIC JI Ecol. Indic. PD JUL PY 2016 VL 66 BP 1 EP 9 DI 10.1016/j.ecolind.2015.11.011 PG 9 WC Biodiversity Conservation; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA ED5SJ UT WOS:000388912300001 ER PT J AU Barnhart, B Bostian, M Whittaker, G Grosskopf, S Fare, R AF Barnhart, Brad Bostian, Moriah Whittaker, Gerald Grosskopf, Shawna Faere, Rolf TI Prioritizing conservation for the reduction of Gulf hypoxia using an environmental performance index SO ECOLOGICAL INDICATORS LA English DT Article DE Agri-environmental policy; Malmquist index; Gulf hypoxia; Mississippi/Atchafalaya River Basin ID MISSISSIPPI/ATCHAFALAYA RIVER-BASIN; OF-MEXICO; MISSISSIPPI RIVER; POWER-PLANTS; MODEL; EFFICIENCY; FIRMS; ZONE AB The annual growth of hypoxia in the Gulf of Mexico is largely attributed to agricultural nutrient loadings that originate from the Mississippi/Atchafalaya River Basin (MARB). To effectively target conservation efforts throughout the entire MARB in order to reduce Gulf hypoxia, strategies to rank areas according to their impact on both agricultural production and ecosystem services are extremely important. In this paper, we utilize an Environmental Performance Index (EPI) to rank regions within the MARB according to their environmental performance, that is, their ability to produce agricultural outputs while minimizing nutrient loadings to the Gulf of Mexico. We compare our index rankings to previously used rankings of delivered yields alone and find the spatial distribution of rankings changes considerably when accounting for agricultural productivity. For example, the Corn Belt regions of central Iowa and northern Illinois no longer make up the lowest performing regions of the MARB after accounting for their high levels of agricultural production. Instead, regions along the Missouri river including central Missouri, western Iowa, and southeastern South Dakota as well as areas near the Ohio river including southern Illinois, western Kentucky, and southern Ohio now count among the lowest performing regions using the EPI ranking scheme. We suggest that incorporation of economic production value into large-scale prioritization of agricultural conservation within the MARB is essential to effectively reduce Gulf hypoxia while maintaining food security from efficient farm production. Published by Elsevier Ltd. C1 [Barnhart, Brad; Whittaker, Gerald] USDA, Agr Res Serv, Corvallis, OR 97331 USA. [Bostian, Moriah] Lewis & Clark Coll, Dept Econ, Portland, OR 97219 USA. [Grosskopf, Shawna; Faere, Rolf] Oregon State Univ, Dept Econ, Corvallis, OR 97331 USA. RP Barnhart, B (reprint author), USDA, Agr Res Serv, Corvallis, OR 97331 USA. EM bradleybarnhart@gmail.com NR 41 TC 1 Z9 1 U1 9 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1470-160X EI 1872-7034 J9 ECOL INDIC JI Ecol. Indic. PD JUL PY 2016 VL 66 BP 235 EP 241 DI 10.1016/j.ecolind.2016.01.002 PG 7 WC Biodiversity Conservation; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA ED5SJ UT WOS:000388912300024 ER PT J AU Byrd, SA Collins, GD Culpepper, AS Dodds, DM Edmisten, KL Wright, DL Morgan, GD Baumann, PA Dotray, PA Manuchehri, MR Jones, A Grey, TL Webster, TM Davis, JW Whitaker, JR Roberts, PM Snider, JL Porter, WM AF Byrd, Seth A. Collins, Guy D. Culpepper, A. Stanley Dodds, Darrin M. Edmisten, Keith L. Wright, David L. Morgan, Gaylon D. Baumann, Paul A. Dotray, Peter A. Manuchehri, Misha R. Jones, Andrea Grey, Timothy L. Webster, Theodore M. Davis, Jerry W. Whitaker, Jared R. Roberts, Phillip M. Snider, John L. Porter, Wesley M. TI Cotton Stage of Growth Determines Sensitivity to 2,4-D SO WEED TECHNOLOGY LA English DT Article DE Crop maturity; Enliste (TM) technology; epinasty; boll distribution ID HORSEWEED CONYZA-CANADENSIS; WEED MANAGEMENT; SIMULATED DRIFT; DICAMBA DRIFT; HERBICIDES; EXPOSURE; YIELD; INJURY AB The anticipated release of Enlist (TM) cotton, corn, and soybean cultivars likely will increase the use of 2,4-D, raising concerns over potential injury to susceptible cotton. An experiment was conducted at 12 locations over 2013 and 2014 to determine the impact of 2,4-D at rates simulating drift (2 g ae ha(-1)) and tank contamination (40 g ae ha(-1)) on cotton during six different growth stages. Growth stages at application included four leaf (4-lf), nine leaf (9-lf), first bloom (FB), FB+2 wk, FB+4 wk, and FB+6 wk. Locations were grouped according to percent yield loss compared to the nontreated check (NTC), with group I having the least yield loss and group III having the most. Epinasty from 2,4-D was more pronounced with applications during vegetative growth stages. Importantly, yield loss did not correlate with visual symptomology, but more closely followed effects on boll number. The contamination rate at 9-lf, FB, or FB+2 wk had the greatest effect across locations, reducing the number of bolls per plant when compared to the NTC, with no effect when applied at FB+4 wk or later. A reduction of boll number was not detectable with the drift rate except in group III when applied at the FB stage. Yield was influenced by 2,4-D rate and stage of cotton growth. Over all locations, loss in yield of greater than 20% occurred at 5 of 12 locations when the drift rate was applied between 4-lf and FB+2 wk (highest impact at FB). For the contamination rate, yield loss was observed at all 12 locations; averaged over these locations yield loss ranged from 7 to 66% across all growth stages. Results suggest the greatest yield impact from 2,4-D occurs between 9-lf and FB 2 wk, and the level of impact is influenced by 2,4-D rate, crop growth stage, and environmental conditions. C1 [Byrd, Seth A.] Texas A&M Univ, Dept Soil & Crop Sci, Lubbock, TX 79403 USA. [Collins, Guy D.] North Carolina State Univ, Dept Crop Sci, Rocky Mount, NC 27801 USA. [Grey, Timothy L.; Whitaker, Jared R.; Snider, John L.; Porter, Wesley M.] Univ Georgia, Dept Crop & Soil Sci, Tifton, GA 31793 USA. [Dodds, Darrin M.] Mississippi State Univ, Dept Plant & Soil Sci, Starkville, MS 39762 USA. [Edmisten, Keith L.] North Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Wright, David L.] Univ Florida, Dept Agron, Quincy, FL 32351 USA. [Morgan, Gaylon D.; Baumann, Paul A.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Dotray, Peter A.; Manuchehri, Misha R.] Texas Tech Univ, Lubbock, TX 79409 USA. [Jones, Andrea] Univ Missouri, Dept Plant Sci, Portageville, MO 63873 USA. [Webster, Theodore M.] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. [Davis, Jerry W.] Univ Georgia, Expt Stat, Griffin, GA 30223 USA. [Roberts, Phillip M.] Univ Georgia, Dept Entomol, Tifton, GA USA. RP Byrd, SA (reprint author), Texas A&M Univ, Dept Soil & Crop Sci, Lubbock, TX 79403 USA. EM seth.byrd@ag.tamu.edu FU Georgia Cotton Commission; Texas State Support Committee; North Carolina Cotton Producers Association; Cotton Incorporated FX This research was graciously funded by the Georgia Cotton Commission, Texas State Support Committee, North Carolina Cotton Producers Association, and Cotton Incorporated. The authors would also like to thank the staff of all the research facilities utilized in this study for their support and assistance with data collection and maintenance of the project, and Dr. Robert Nichols for his time in reviewing the manuscript. NR 26 TC 0 Z9 0 U1 2 U2 2 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0890-037X EI 1550-2740 J9 WEED TECHNOL JI Weed Technol. PD JUL-SEP PY 2016 VL 30 IS 3 BP 601 EP 610 DI 10.1614/WT-D-15-00191.1 PG 10 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DX3SS UT WOS:000384294100001 ER PT J AU Spring, JF Boydston, RA Burke, IC AF Spring, John F. Boydston, Rick A. Burke, Ian C. TI Photosystem II Inhibitor Resistance in the Columbia Basin of Washington State SO WEED TECHNOLOGY LA English DT Article DE Herbicide resistance; photosystem II; psbA gene; Ser(264)Gly mutation ID AMARANTH AMARANTHUS-POWELLII; CHENOPODIUM-ALBUM; CROSS-RESISTANCE; HERBICIDE RESISTANCE; SOLANUM-TUBEROSUM; TARGET SITE; IDENTIFICATION; TRIAZINE; RETROFLEXUS; METRIBUZIN AB Weed management in potato and mint rely heavily on two Group 5 photosystem II-inhibiting herbicides, metribuzin and terbacil, respectively. Seed from weed escapes was collected in 2010 from 69 potato and mint production fields in Washington State and tested for resistance to metribuzin and terbacil. Of the seed screened, 26 of 51 Powell amaranth and redroot pigweed accessions and 8 of 23 common lambsquarters accessions tested expressed at least some degree of resistance to one or both herbicides. A Ser(264)Gly mutation in the chloroplast psbA gene was present in all but three resistant accessions. Herbicides with other mechanisms of action registered in potato and mint controlled all identified photosystem II-resistant accessions when applied at typical labeled rates. C1 [Spring, John F.; Burke, Ian C.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Boydston, Rick A.] ARS, USDA, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. RP Spring, JF (reprint author), Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. EM john.spring@wsu.edu FU Washington Mint Commission; Washington Potato Commission FX The authors thank Treva Anderson, Raymond Baker, Sheri Rynearson, and Attawan Aramrak for their excellent technical assistance, and Drew Lyon for helpful review of the manuscript. The authors also thank the Washington Mint Commission and the Washington Potato Commission for partial funding of the study. Thanks also to the two anonymous reviewers for substantial improvement of the manuscript. NR 28 TC 0 Z9 0 U1 1 U2 1 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0890-037X EI 1550-2740 J9 WEED TECHNOL JI Weed Technol. PD JUL-SEP PY 2016 VL 30 IS 3 BP 783 EP 792 DI 10.1614/WT-D-15-00138.1 PG 10 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DX3SS UT WOS:000384294100021 ER PT J AU Smith, HC Ferrell, JA Webster, TM Fernandez, JV Dittmar, PJ Munoz, PR MacDonald, GE AF Smith, Hunter C. Ferrell, Jason A. Webster, Theodore M. Fernandez, Jose V. Dittmar, Peter J. Munoz, Patricio R. MacDonald, Greg E. TI Impact of Irrigation Volume on PRE Herbicide Activity SO WEED TECHNOLOGY LA English DT Article DE Herbicide resistance management; herbicide-resistant weeds; incorporation; weed control ID AMARANTH AMARANTHUS-PALMERI; GLYPHOSATE-RESISTANT CROPS; COTTON GOSSYPIUM-HIRSUTUM; SOIL INCORPORATION; DICAMBA; FOMESAFEN; PERSISTENCE; METOLACHLOR; MANAGEMENT; MOBILITY AB The importance of PRE herbicide applications in cotton has increased since the evolution of glyphosate-resistant (GR) Palmer amaranth. Cotton producers are relying on residual herbicides for control of Palmer amaranth, as POST options are limited or ineffective. S-Metolachlor, acetochlor, fomesafen, and dicamba all provide PRE control of Palmer amaranth; however, little is known about the effect of irrigation rate on incorporation and herbicidal efficacy. In 2015, an experiment was conducted on fine sand and loamy sand soils to evaluate the influence of irrigation volume (0.0 to 12.7 mm ha(-1)) on Palmer amaranth control with PRE herbicides. Irrigation volume after herbicide application was significant for both S-metolachlor and acetochlor. Efficacy of S-metolachlor was greatest in plots receiving 6.4 and 12.7 mm of irrigation where Palmer amaranth biomass was reduced to 4 and 2% of a nontreated control (NTC), respectively, compared with 61% in plots with the 0-mm irrigation treatment. Palmer amaranth control by acetochlor incorporated at 3.2-to 12.7 mm irrigation did not differ but did reduce Palmer amaranth biomass compared with the 1.6-mm irrigation rate. Irrigation volume was not significant for the soil incorporation of fomesafen or dicamba. Across all herbicides, fomesafen-treated plots provided the most consistent control of Palmer amaranth, reducing its biomass to, 3% of NTC at all irrigation rates. Dicamba provided the least and most inconsistent control of Palmer amaranth, producing 17 to 51% of NTC biomass. C1 [Smith, Hunter C.; Ferrell, Jason A.; Fernandez, Jose V.; Munoz, Patricio R.; MacDonald, Greg E.] Univ Florida, Dept Agron, POB 11111, Gainesville, FL 32611 USA. [Webster, Theodore M.] ARS, Crop Protect & Management Res Unit, USDA, 2747 Davis Rd, Tifton, GA 31793 USA. [Dittmar, Peter J.] Univ Florida, Dept Hort, 1301 Fifield Hall, Gainesville, FL 32611 USA. RP Ferrell, JA (reprint author), Univ Florida, Dept Agron, POB 11111, Gainesville, FL 32611 USA. EM jferrell@ufl.edu NR 36 TC 0 Z9 0 U1 1 U2 1 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0890-037X EI 1550-2740 J9 WEED TECHNOL JI Weed Technol. PD JUL-SEP PY 2016 VL 30 IS 3 BP 793 EP 800 DI 10.1614/WT-D-16-00014.1 PG 8 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DX3SS UT WOS:000384294100022 ER PT J AU Kormos, PR Luce, CH Wenger, SJ Berghuijs, WR AF Kormos, Patrick R. Luce, Charles H. Wenger, Seth J. Berghuijs, Wouter R. TI Trends and sensitivities of low streamflow extremes to discharge timing and magnitude in Pacific Northwest mountain streams SO WATER RESOURCES RESEARCH LA English DT Article ID WESTERN UNITED-STATES; FRESH-WATER ECOSYSTEMS; CLIMATE-CHANGE IMPACTS; MEAN ANNUAL RUNOFF; PATH-ANALYSIS; LOW FLOWS; POTENTIAL EVAPOTRANSPIRATION; IRRIGATED AGRICULTURE; HYDROLOGICAL DROUGHT; SPATIAL VARIABILITY AB Path analyses of historical streamflow data from the Pacific Northwest indicate that the precipitation amount has been the dominant control on the magnitude of low streamflow extremes compared to the air temperature-affected timing of snowmelt runoff. The relative sensitivities of low streamflow to precipitation and temperature changes have important implications for adaptation planning because global circulation models produce relatively robust estimates of air temperature changes but have large uncertainties in projected precipitation amounts in the Pacific Northwest U.S. Quantile regression analyses indicate that low streamflow extremes from the majority of catchments in this study have declined from 1948 to 2013, which may significantly affect terrestrial and aquatic ecosystems, and water resource management. Trends in the 25th percentile of mean annual streamflow have declined and the center of timing has occurred earlier. We quantify the relative influences of total precipitation and air temperature on the annual low streamflow extremes from 42 stream gauges using mean annual streamflow as a proxy for precipitation amount effects and streamflow center of timing as a proxy for temperature effects on low flow metrics, including 7q10 summer (the minimum 7 day flow during summer with a 10 year return period), mean August, mean September, mean summer, 7q10 winter, and mean winter flow metrics. These methods have the benefit of using only readily available streamflow data, which makes our results robust against systematic errors in high elevation distributed precipitation data. Winter low flow metrics are weakly tied to both mean annual streamflow and center of timing. C1 [Kormos, Patrick R.; Luce, Charles H.] US Forest Serv, Rocky Mt Res Stn, Boise, ID 83709 USA. [Kormos, Patrick R.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN 37830 USA. [Kormos, Patrick R.] US Agr Res Serv, Northwest Watershed Res Ctr, Boise, ID 83712 USA. [Wenger, Seth J.] Univ Georgia, Odum Sch Ecol, River Basin Ctr, Athens, GA 30602 USA. [Berghuijs, Wouter R.] Univ Bristol, Dept Civil Engn, Bristol, Avon, England. RP Kormos, PR (reprint author), US Forest Serv, Rocky Mt Res Stn, Boise, ID 83709 USA.; Kormos, PR (reprint author), Oak Ridge Inst Sci & Educ, Oak Ridge, TN 37830 USA.; Kormos, PR (reprint author), US Agr Res Serv, Northwest Watershed Res Ctr, Boise, ID 83712 USA. EM patrick.kormos@ars.usda.gov RI Luce, Charles/A-9267-2008; OI Luce, Charles/0000-0002-6938-9662; Berghuijs, Wouter/0000-0002-7447-0051 FU DOE [DE-AC05-06OR23100]; NASA [NNX14AC91G] FX We thank USDA-ARS Northwest Watershed Research Center and Boise State University for general support. Data used in this study are available from the resources cited in the article text. This research was supported in part by an appointment to the U.S. Forest Service Research Participation Program administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and the U.S. Department of Agriculture Forest Service. ORISE is managed by the Oak Ridge Associated Universities (ORAU) under DOE contract number DE-AC05-06OR23100. This work is also partially funded by NASA grand number NNX14AC91G. All opinions expressed in this paper are the author's and do not necessarily reflect the policies and views of USDA, DOE, or ORAU/ORISE. USDA is an equal opportunity provider and employer. NR 144 TC 0 Z9 0 U1 17 U2 17 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD JUL PY 2016 VL 52 IS 7 BP 4990 EP 5007 DI 10.1002/2015WR018125 PG 18 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA DW5KO UT WOS:000383683800002 ER PT J AU Alvarez-Garreton, C Ryu, D Western, AW Crow, WT Su, CH Robertson, DR AF Alvarez-Garreton, Camila Ryu, Dongryeol Western, Andrew W. Crow, Wade T. Su, Chun-Hsu Robertson, David R. TI Dual assimilation of satellite soil moisture to improve streamflow prediction in data-scarce catchments SO WATER RESOURCES RESEARCH LA English DT Article ID RAINFALL-RUNOFF MODEL; IN-SITU OBSERVATIONS; NEAR-SURFACE; PARAMETER-ESTIMATION; ERS SCATTEROMETER; KALMAN FILTER; PRECIPITATION; ASCAT; PRODUCTS; RETRIEVALS AB This paper explores the use of active and passive microwave satellite soil moisture products for improving streamflow prediction within four large (>5000km(2)) semiarid catchments in Australia. We use the probability distributed model (PDM) under a data-scarce scenario and aim at correcting two key controlling factors in the streamflow generation: the rainfall forcing data and the catchment wetness condition. The soil moisture analysis rainfall tool (SMART) is used to correct a near real-time satellite rainfall product (forcing correction scheme) and an ensemble Kalman filter is used to correct the PDM soil moisture state (state correction scheme). These two schemes are combined in a dual correction scheme and we assess the relative improvements of each. Our results demonstrate that the quality of the satellite rainfall product is improved by SMART during moderate-to-high daily rainfall events, which in turn leads to improved streamflow prediction during high flows. When employed individually, the soil moisture state correction scheme generally outperforms the rainfall correction scheme, especially for low flows. Overall, the combined dual correction scheme further improves the streamflow predictions (reduction in root mean square error and false alarm ratio, and increase in correlation coefficient and Nash-Sutcliffe efficiency). Our results provide new evidence of the value of satellite soil moisture observations within data-scarce regions. We also identify a number of challenges and limitations within the schemes. C1 [Alvarez-Garreton, Camila; Ryu, Dongryeol; Western, Andrew W.; Su, Chun-Hsu] Univ Melbourne, Dept Infrastruct Engn, Parkville, Vic, Australia. [Alvarez-Garreton, Camila] Univ Austral Chile, Inst Conversac Biodiversidad & Terr, Lab Dendrocronol & Cambio Global, Valdivia, Chile. [Alvarez-Garreton, Camila] Ctr Climate & Resilience Res CR2, Santiago, Chile. [Crow, Wade T.] ARS, USDA, Hydrol & Remote Sensing Lab, Beltsville, MD USA. [Robertson, David R.] CSIRO Land & Water, Highett, Vic, Australia. RP Alvarez-Garreton, C (reprint author), Univ Melbourne, Dept Infrastruct Engn, Parkville, Vic, Australia.; Alvarez-Garreton, C (reprint author), Univ Austral Chile, Inst Conversac Biodiversidad & Terr, Lab Dendrocronol & Cambio Global, Valdivia, Chile.; Alvarez-Garreton, C (reprint author), Ctr Climate & Resilience Res CR2, Santiago, Chile. EM calvarezgarreton@gmail.com RI Western, Andrew/G-1730-2011; Robertson, David/C-3643-2011; Alvarez Garreton, Camila/M-1232-2016 OI Western, Andrew/0000-0003-4982-146X; Robertson, David/0000-0003-4230-8006; Alvarez Garreton, Camila/0000-0002-5381-4863 FU Australian Research Council (ARC) [LP110200520]; Bureau of Meteorology, Australia; Becas Chile scholarship; [CONICYT/FONDAP/1511000] FX This research was conducted with financial support from the Australian Research Council (ARC Linkage Project No. LP110200520) and the Bureau of Meteorology, Australia. C. Alvarez-Garreton was supported by a Becas Chile scholarship and by CONICYT/FONDAP/1511000. We are grateful to all who contributed to the data sets used in this study. We thank Chris Leahy and Soori Sooriyakumaran from the Australian Bureau of Meteorology for providing catchment data and gratefully acknowledge their advice. AWAP rainfall data was downloaded from http://www.bom.gov.au/jsp/awap/rain/archive.jsp. AMSR-E data were downloaded from ftp://hydro1.sci.gsfc.nasa.gov/data/s4pa/WAOB/LPRM_AMSRE_A_SOILM3.002/ (see also http://gcmd.nasa.gov). ASCAT data can be downloaded from http://hsaf.meteoam.it/soil-moisture.php. The SMOS data were downloaded from http://www.catds.fr/Products/Available-products-from-CPDC. The TMPA data were downloaded from http://disc2.nascom.nasa.gov/opendap/TRMM_L3/TRMM_3B42/. We also thank the anonymous reviewers and associate editor for their comments which have improved the quality of this paper. NR 62 TC 1 Z9 1 U1 1 U2 1 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD JUL PY 2016 VL 52 IS 7 BP 5357 EP 5375 DI 10.1002/2015WR018429 PG 19 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA DW5KO UT WOS:000383683800024 ER PT J AU Kumar, AK Parikh, BS Shah, E Liu, LZ Cotta, MA AF Kumar, Adepu K. Parikh, Bhumika S. Shah, Ekta Liu, Lewis Z. Cotta, Michael A. TI Cellulosic ethanol production from green solvent-pretreated rice straw SO BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY LA English DT Article DE NADES; Green solvents; Clavispora NRRL Y-50464; Cellic ctec2; Stability; Cellulosic ethanol ID DEEP EUTECTIC SOLVENTS; IONIC LIQUID; CHOLINE CHLORIDE; LACTIC-ACID; HYDROLYSIS; CONVERSION; BIOMASS; YEAST; WATER AB Cellulosic ethanol production from green solvent (GS) -pretreated rice straw and the effects of green solvents on cellulase enzyme cellic ctec2 and cellobiose-fermenting yeast strain Clavispora NRRL Y-50464 was comprehensively investigated. Using choline chloride/glycerol (CC-GLY) treated rice straw, maximum reducing sugars of 226.7 g/L was obtained with a sacchariflcation efficiency of 87.1% at 20% solids loading and 12 FPU cellic ctec2. An ethanol production of 36.7 g/L was observed from 8% of glucose within 36 h with a conversion efficiency of 90.1%. Incubation of cellulase in green solvents (CC-GLY, choline chloride/1,2-propane diol (CC-PD), choline chloride/ethylene glycol (CC-EG)) at high concentrations (up to 30%, v/v) had no inhibitory effect on cellic ctec2. Moreover, CC-GLY and CC-PD at 10% (v/v) did not affect the growth rate, sugar consumption and ethanol production from Clavispora NRRL Y-50464, while 10% (v/v) CC-EG repressed and delayed the cell growth of the microbe. While, strain Y-50464 was highly sensitive in other green reagents that were evaluated in this study. The green solvent-pretreatment efficiently removed lignin from the ricestraw and improved fermentation efficiency significantly. Green solvents with pH < 3.0 inhibited of cellic ctec2 enzyme activity and growth of Clavispora strain NRRL Y-50464. When pH of the green reagents was neutralized or adjusted to pH 5.0, normal enzyme activity and cell growth was observed. Moreover, with selective green reagents i.e. CC-GLY, CC-PD, and CC-EG, cellulosic ethanol production can be accomplished using cellic ctec2 and strain NRRL Y-50464. (C) 2016 Elsevier Ltd All rights reserved. C1 [Kumar, Adepu K.; Parikh, Bhumika S.; Shah, Ekta] Near BVM Engn Coll, Sardar Patel Renewable Energy Res Inst, Bioconvers Technol Div, Post Box 2, Vallabh Vidyanagar 388120, Gujarat, India. [Liu, Lewis Z.; Cotta, Michael A.] USDA ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Kumar, AK (reprint author), Near BVM Engn Coll, Sardar Patel Renewable Energy Res Inst, Bioconvers Technol Div, Post Box 2, Vallabh Vidyanagar 388120, Gujarat, India. EM kiranbio@gmail.com NR 27 TC 0 Z9 0 U1 3 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1878-8181 J9 BIOCATAL AGRIC BIOTE JI Biocatal. Agric. Biotechnol. PD JUL PY 2016 VL 7 BP 14 EP 23 DI 10.1016/j.bcab.2016.04.008 PG 10 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA ED1EF UT WOS:000388586500003 ER PT J AU Peralta, DV Fortier, CA Delhom, CD Rodgers, JE Thibodeaux, DP AF Peralta, Donna V. Fortier, Chanel A. Delhom, Christopher D. Rodgers, James E. Thibodeaux, Devron P. TI Separation and Quantitation of Plant and Insect Carbohydrate Isomers Found on the Surface of Cotton SO AATCC JOURNAL OF RESEARCH LA English DT Article DE Cotton Processing; Cotton Stickiness; Ion Chromatography; Sugars ID SPECTROSCOPY; TREHALULOSE; METABOLISM; STICKINESS; PROFILES; SUCROSE; HONEYS; HPLC AB Certain levels of the carbohydrates melezitose and trehalulose deposited on cotton surfaces are indicative of either whitefly or aphid contamination, which may cause problems during cotton processing. Raffinose and sucrose are isomers of melezitose and trehalulose, respectively, making it difficult to fully separate them via ion chromatography (IC), especially when analysis time is shortened. We have successfully developed an IC method to separate the retention peaks of melezitose from raffinose and trehalulose from sucrose, with baseline resolution and improved quantitation of these sugars. This improved separation may elucidate useful information about constituent sugars on cotton, aiding in the identification of carbohydrates possibly contributing to stickiness. C1 [Peralta, Donna V.] ARS, USDA, Cotton Struct & Qual CSQ Res Unit, SRRC, New Orleans, LA USA. [Fortier, Chanel A.; Delhom, Christopher D.; Rodgers, James E.] ARS, USDA, New Orleans, LA USA. [Thibodeaux, Devron P.] Fiber Phys LLC, Metairie, LA USA. RP Peralta, DV (reprint author), ARS, USDA, SRRC, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM Donna.Peralta@ars.usda.gov NR 27 TC 0 Z9 0 U1 2 U2 2 PU AMER ASSOC TEXTILE CHEMISTS COLORISTS PI RES TRIANGLE PK PA PO BOX 12215, RES TRIANGLE PK, NC 27709 USA SN 2330-5517 J9 AATCC J RES JI AATCC J. Res. PD JUL-AUG PY 2016 VL 3 IS 4 BP 13 EP 23 DI 10.14504/ajr.3.4.2 PG 11 WC Materials Science, Textiles SC Materials Science GA EB8OK UT WOS:000387650300002 ER PT J AU da Motta, PEF Siqueira, JO Ribeiro, BT Norton, LD Silva, SHG Curi, N AF Ferreira da Motta, Paulo Emilio Siqueira, Jose Oswaldo Ribeiro, Bruno Teixeira Norton, Lloyd Darrell Godinho Silva, Sergio Henrique Curi, Nilton TI Long-term phosphate fertilization, mycorrhizal inoculation and historical land use influence on soybean growth and P uptake SO CIENCIA E AGROTECNOLOGIA LA English DT Article DE Mycorrhiza; Latosols (Oxisols); available P; long-term cultivation ID PHOSPHORUS-NUTRITION; NUTRIENT-UPTAKE; FUNGI; SOIL; COLONIZATION; AGRICULTURE; DETERMINES; BENEFITS; IMPROVE; OXISOLS AB Phosphorus is a key-nutrient in the fertility management of highly weathered tropical soils. So, this work was carried out with the objective of evaluating the influence of the interaction between P doses, mycorrhizal inoculation and historical land use on soybean growth and P uptake in five Latosols (Oxisols) with contrasting chemical, physical and mineralogical properties under a continuous long-term phosphate fertilization (more than 15 years). The plants were cultivated in 4.5L-plastic pots containing 4 kg of soil in a completely randomized design, four replications and 2x2x2 factorial scheme with two P doses; and with or without mycorrhizal inoculation; and soils cultivated for long periods and non-cultivated (under native vegetation). There were two cultivations of ten weeks each. Shoot dry mass, P content and accumulation in the shoot dry mass were evaluated after each cultivation period. The cultivation history reduced the response to P application and inoculation. The soybean response to inoculation was greater in cultivated soils and when the lower P dose was applied. The soybean response magnitude to these variables was different among the studied Latosols. The mineralogical and chemical attributes of the Latosols were determinants. C1 [Ferreira da Motta, Paulo Emilio] Empresa Brasileira Pesquisa Agr Embrapa Solos, Rio De Janeiro, RJ, Brazil. [Siqueira, Jose Oswaldo] Inst Tecnol Vale, Belem, Para, Brazil. [Ribeiro, Bruno Teixeira; Curi, Nilton] Univ Fed Lavras UFLA, DCS, Lavras, MG, Brazil. [Norton, Lloyd Darrell] USDA ARS, Natl Soil Eros Res Lab, W Lafayette, IN USA. [Godinho Silva, Sergio Henrique] Univ Fed Lavras UFLA, Lavras, MG, Brazil. RP Ribeiro, BT (reprint author), Univ Fed Lavras UFLA, DCS, Lavras, MG, Brazil. EM brunoribeiro@dcs.ufla.br NR 33 TC 0 Z9 0 U1 8 U2 8 PU UNIV FEDERAL LAVRAS-UFLA PI LAVRAS PA CAIXA POSTAL 3037, LAVRAS, MG 37200-000, BRAZIL SN 1413-7054 EI 1981-1829 J9 CIENC AGROTEC JI Cienc. Agrotec. PD JUL-AUG PY 2016 VL 40 IS 4 BP 418 EP 431 DI 10.1590/1413-70542016404014216 PG 14 WC Agriculture, Multidisciplinary; Agronomy SC Agriculture GA DW3PX UT WOS:000383555700005 ER PT J AU Safeeq, M Shukla, S Arismendi, I Grant, GE Lewis, SL Nolin, A AF Safeeq, Mohammad Shukla, Shraddhanand Arismendi, Ivan Grant, Gordon E. Lewis, Sarah L. Nolin, Anne TI Influence of winter season climate variability on snow-precipitation ratio in the western United States SO INTERNATIONAL JOURNAL OF CLIMATOLOGY LA English DT Article DE snow fraction; climate warming; signal-to-noise ratio; trend analysis; western United States ID DECLINING MOUNTAIN SNOWPACK; PACIFIC-NORTHWEST; STREAMFLOW TRENDS; CHANGING CLIMATE; RIVER-BASIN; TEMPERATURE; CALIFORNIA; AMERICA; IMPACTS; CONSEQUENCES AB In the western United States, climate warming poses a unique threat to water and snow hydrology becausemuch of the snowpack accumulates at temperatures near 0 degrees C. As the climate continues to warm, much of the region's precipitation is expected to switch from snow to rain, causing flashier hydrographs, earlier inflow to reservoirs, and reduced spring and summer snowpack. This study investigates historical variability in snow to precipitation proportion (S-f) and maps areas in the western United States that have demonstrated higher S-f sensitivity to warming in the past. Projected changes in S-f under 1.1, 1.8, and 3.0 degrees C future warming scenarios are presented in relation to historical variability and sensitivity. Our findings suggest that S-f in this region has primarily varied based on winter temperature rather than precipitation. The difference in S-f between cold and warm winters at low-and mid-elevations during 1916-2003 ranged from 31% in the Pacific Northwest to 40% in the California Sierra Nevada. In contrast, the difference in S-f between wet and dry winters was statistically not significant. Overall, in the northern Sierra, Klamath, and western slopes of the Cascade Mountains Ranges, S-f was most sensitive to temperature where winter temperature ranged between -5 to 5 degrees C. Results from our trend analysis show a regional shift in both S-f and signal-to-noise ratios during 1960-2003 as compared with 1916-2003. Our findings indicate that natural variability in S-f over 1916-2003 across all regions except for the Great Basin most closely resembles the projected 2040-warming scenario (+1.8 degrees C). C1 [Safeeq, Mohammad] Univ Calif, Sierra Nevada Res Inst, 5200 N Lake Rd, Merced, CA 95343 USA. [Safeeq, Mohammad] US Forest Serv, USDA, PSW Res Stn, Fresno, CA USA. [Shukla, Shraddhanand] Univ Calif Santa Barbara, Dept Geog, Santa Barbara, CA 93106 USA. [Grant, Gordon E.] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Grant, Gordon E.] US Forest Serv, USDA, PNW Res Stn, Corvallis, OR USA. [Lewis, Sarah L.; Nolin, Anne] Oregon State Univ, Coll Earth Ocean & Atmospher Sci, Corvallis, OR 97331 USA. RP Safeeq, M (reprint author), Univ Calif, Sierra Nevada Res Inst, 5200 N Lake Rd, Merced, CA 95343 USA. EM msafeeq@ucmerced.edu NR 63 TC 4 Z9 4 U1 12 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0899-8418 EI 1097-0088 J9 INT J CLIMATOL JI Int. J. Climatol. PD JUL PY 2016 VL 36 IS 9 BP 3175 EP 3190 DI 10.1002/joc.4545 PG 16 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DW4JC UT WOS:000383608000002 ER PT J AU Ghimire, R Green, GT Poudyal, NC Cordell, HK AF Ghimire, Ramesh Green, Gary T. Poudyal, Neelam C. Cordell, H. Ken TI Who Recreates Where: Implications from a National Recreation Household Survey SO JOURNAL OF FORESTRY LA English DT Article DE consumptive and nonconsumptive recreation; land-based and water-based recreation; National Survey on Recreation and the Environment (NSRE); private and public forests ID OUTDOOR RECREATION; GREEN SPACE; STRATIFICATION; PARTICIPATION; PREFERENCES; LANDS; RACE AB Given the growing US population and its relatively stable supply of publicly owned forests, it seems likely that future demand for outdoor recreation will be increasingly satisfied by privately owned forests. Therefore, it becomes important to understand whether visitors to publicly and privately owned forests have different characteristics. Using data from a US household survey, we found that college graduates and water-based consumptive recreationists ( e.g., fishermen) were more likely to recreate on publicly owned forests and females and land-based consumptive recreationists ( e.g., hunters) were more likely to recreate on privately owned forests. Our findings also suggested that elderly people and ethnic minorities appeared to be underutilizing public recreation lands in the United States. C1 [Ghimire, Ramesh; Green, Gary T.] Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. [Poudyal, Neelam C.] Univ Tennessee, Dept Forestry Wildlife & Fisheries, Knoxville, TN USA. [Cordell, H. Ken] US Forest Serv, USDA, Southern Res Stn, Athens, GA USA. RP Ghimire, R (reprint author), Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. EM ghimire@uga.edu; gtgreen@uga.edu; npoudyal@utk.edu; haroldcordell@fs.fed.us NR 59 TC 0 Z9 0 U1 0 U2 0 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD JUL PY 2016 VL 114 IS 4 BP 458 EP 465 DI 10.5849/jof.14-106 PG 8 WC Forestry SC Forestry GA DW1WE UT WOS:000383433200004 ER PT J AU Sorenson, CB Keegan, CE Morgan, TA McIver, CP Niccolucci, MJ AF Sorenson, Colin B. Keegan, Charles E., III Morgan, Todd A. McIver, Chelsea P. Niccolucci, Michael J. TI Employment and Wage Impacts of Timber Harvesting and Processing in the United States SO JOURNAL OF FORESTRY LA English DT Review DE direct response coefficients; employment ratios; forest economics; forest industry; timber product output AB Each unit of timber has some number of jobs and income associated with it. We developed regional estimates of employment per million cubic feet of timber harvested, referred to as employment direct response coefficients ( DRCs), and wages per worker in 16 sectors of the US primary forest products industry. Estimates are based on timber product output and federal employment data. These estimates offer comparisons of direct timber processing employment associated with various sectors and geographic regions. They also provide potentially more accurate direct impacts that can be used in conjunction with input-output analysis to estimate indirect and induced impacts. Results show substantial differences among regions and industry sectors. The lowest employment DRCs among timber processing sectors are in the biomass energy and oriented-strandboard sectors. The hardwood plywood/ veneer, log home, and log furniture sectors have the highest employment ratios. Workers in the pulp and paper sector are among the highest paid in the forest products industry. The capital intensity of the facilities, the variety of products produced, and the degree of manufacturing contribute to ratio differences among sectors and regions. C1 [Sorenson, Colin B.] Univ Montana, Bur Business & Econ Res, Missoula, MT 59812 USA. [Keegan, Charles E., III; Morgan, Todd A.; McIver, Chelsea P.] Univ Montana, Missoula, MT 59812 USA. [Niccolucci, Michael J.] US Forest Serv, USDA, Washington, DC USA. RP Sorenson, CB (reprint author), Univ Montana, Bur Business & Econ Res, Missoula, MT 59812 USA. EM colin.sorenson@business.umt.edu; charles.keegan@business.umt.edu; todd.morgan@business.umt.edu; chelsea.mciver@business.umt.edu; mniccolucci@fs.fed.us FU USDA Forest Service, Ecosystem Management Coordination office [12-CS-11132427-325] FX We acknowledge the USDA Forest Service, Ecosystem Management Coordination office for supporting this research under Agreement No. 12-CS-11132427-325. We also thank the Forest Inventory and Analysis Program, particularly James W. Bentley of the Southern Research Station in Knoxville, TN, and Ronald J. Piva of the Northern Research Station in St. Paul, MN, for providing information and assistance. NR 28 TC 0 Z9 0 U1 1 U2 1 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD JUL PY 2016 VL 114 IS 4 BP 474 EP 482 DI 10.5849/jof.14-082 PG 9 WC Forestry SC Forestry GA DW1WE UT WOS:000383433200006 ER PT J AU Wear, DN Prestemon, JP Foster, MO AF Wear, David N. Prestemon, Jeffrey P. Foster, Michaela O. TI US Forest Products in the Global Economy SO JOURNAL OF FORESTRY LA English DT Review DE forest sector; industrial roundwood; market share; timber products AB The United States' shares of global industrial roundwood production and derivative products have declined precipitously since the 1990s. We evaluate the extent of these declines compared with those of major producing countries from 1961 to 2013. We find that the US global share of industrial roundwood peaked at 28% in 1999 but by 2013 was at 17%, with the decline attributable to a combination of cyclical factors and long-run trends. Wood products output declines are linked to low construction levels, whereas paper's decline is connected to the offshoring of US manufacturing and growth in electronic media. Prospects are for increased use of wood in construction as the housing market returns to long-run averages in the coming years. However, the paper sector is unlikely to recover to its 1990s levels of output, implying that it is unlikely that the United States will return to its historical highs in global market share of industrial roundwood production. C1 [Wear, David N.] US Forest Serv, USDA, So Res Stn, Res Triangle Pk, NC 20250 USA. [Prestemon, Jeffrey P.; Foster, Michaela O.] US Forest Serv, USDA, Res Triangle Pk, NC USA. RP Wear, DN (reprint author), US Forest Serv, USDA, So Res Stn, Res Triangle Pk, NC 20250 USA. EM dwear@fs.fed.us; jprestemon@fs.fed.us; mofoster@fs.fed.us NR 30 TC 1 Z9 1 U1 1 U2 1 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD JUL PY 2016 VL 114 IS 4 BP 483 EP 493 DI 10.5849/jof.15-091 PG 11 WC Forestry SC Forestry GA DW1WE UT WOS:000383433200007 ER PT J AU Madsen, JD Wersal, RM Marko, MD AF Madsen, John D. Wersal, Ryan M. Marko, Michelle D. TI Distribution and Biomass Allocation in Relation to Depth of Flowering Rush (Butomus umbellatus) in the Detroit Lakes, Minnesota SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Aquatic invasive species; aquatic macrophyte; biomass allocation; depth distribution; emergent plant; littoral zone plant ID PLANT COMMUNITY INVASIBILITY; ST-LAWRENCE-RIVER; AQUATIC PLANT; ECOSYSTEM ENGINEERS; CLONAL REPRODUCTION; WATER-LEVEL; EURASIAN WATERMILFOIL; NATIVE VEGETATION; EXTREME VARIATION; SHALLOW LAKE AB The Detroit Lakes chain of lakes consists of five basins in northwest Minnesota adjacent to the town of Detroit Lakes. Flowering rush has been established in these basins since the 1960s. We evaluated the distribution of flowering rush in the five basins using a point intercept method, with 830 points distributed in a grid with points 150 m apart. These data were analyzed to determine whether invasive and native species frequencies were different between 2010 and 2011. We also assessed co-occurrence of flowering rush with native hardstem bulrush. The distribution of both flowering rush and hardstem bulrush was unchanged from 2010 to 2011. Flowering rush is invading areas with native plants and not establishing in unvegetated areas. Although flowering rush is found as deep as 4.5 m, it is most frequent at a depth of 1.3 m. We also examined the distribution of biomass and growth across a depth gradient from 0.3 to 3.0 m in 0.3-m intervals. At each 0.3-m interval, three biomass samples were collected at each of 10 transects for a total of 30 samples per depth interval or 300 biomass samples. At each point, leaf height, emergent leaf height, water depth, number of ramets, and number of rhizome buds were counted. Biomass samples were collected in a 0.018-m(2) core sampler, sorted to shoots and belowground biomass. We found that flowering rush height and biomass peaked at 1.3 m and declined with greater depth. Bud density was negatively related to water depth. Bud density averaged 300 buds m(-2), which was three times the average ramet density (100 ramets m(-2)). C1 [Madsen, John D.] Univ Calif Davis, USDA ARS EIW, Dept Plant Sci, Davis, CA 95616 USA. Lonza, 1200 Bluegrass Lakes Pkwy, Alpharetta, GA 30004 USA. Concordia Coll, Dept Biol, 901 8th St S, Moorhead, MN 56562 USA. RP Madsen, JD (reprint author), Univ Calif Davis, USDA ARS EIW, Dept Plant Sci, Davis, CA 95616 USA. EM jmadsen@ucdavis.edu FU Pelican River Watershed District; Minnesota Department of Natural Resources, Invasive Species Program; Minnesota Department of Natural Resources FX Funding for these studies was provided by the Pelican River Watershed District, with additional support from the Minnesota Department of Natural Resources, Invasive Species Program. We thank Bradley Sartain, Gray Turnage, a number of undergraduate students at Concordia College, and the staff of the Pelican River Watershed District for assistance in the field and lab. We thank Tera Guetter (Pelican River Watershed District), Patrick Selter (Professional Lake Management), Darrin Hoverson, Daniel Swanson, and Chip Welling (Minnesota Department of Natural Resources) for information, insight, and preliminary data on the lake. This project was supported by the Pelican River Watershed District, with additional support from the Minnesota Department of Natural Resources. Earlier versions of this manuscript were reviewed by Gary Ervin, Paul Pratt, Gray Turnage, and Charles Welling. Mention of a manufacturer does not constitute a warranty or guarantee of the product by the U.S. Department of Agriculture nor an endorsement over other products not mentioned. NR 63 TC 0 Z9 0 U1 6 U2 6 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 EI 1939-747X J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD JUL-SEP PY 2016 VL 9 IS 3 BP 161 EP 170 DI 10.1614/IPSM-D-15-00028.1 PG 10 WC Plant Sciences SC Plant Sciences GA EA9EF UT WOS:000386943400001 ER PT J AU Averill, KM DiTommaso, A Whitlow, TH Milbrath, LR AF Averill, Kristine M. DiTommaso, Antonio Whitlow, Thomas H. Milbrath, Lindsey R. TI Photosynthetic Performance of Invasive Vincetoxium Species (Apocynaceae) SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Apocynaceae; dog-strangling vine; exotic plants; forest understory; light environment; old field ID NEW-YORK-STATE; V. NIGRUM; GROWTH; ROSSICUM; PLANTS; ASSIMILATION; ACCLIMATION; ENVIRONMENT; INVADERS; SUCCESS AB Knowledge of photosynthetic capacity is crucial for fully understanding a species' invasive potential and for the development of appropriate control strategies. Although growth and reproductive data are available for the invasive swallowwort vines Vincetoxicum nigrum and V. rossicum, photosynthetic data are wanting. These herbaceous, perennial congeners were introduced from separate European ranges during the late 19th century and became invasive during the following century in the northeastern United States and southeastern Canada. Vincetoxicum nigrum has been observed growing mainly in high light environments, whereas V. rossicum occurs across a wide range of light environments, suggesting niche divergence and that different management strategies might be needed for the two species. In this work, we investigated whether the differing habitat associations of these species is reflected in their photosynthetic capacities and leaf morphology. Photosynthetic parameters and specific leaf mass were determined across a range of light environments represented by four field habitats (common garden, forest edge, old field, and forest understory) and two greenhouse environments (high and low light). In the high-light common garden habitat, V. nigrum achieved 37% higher maximum photosynthetic rates than V. rossicum, but photosynthetic performance of the two species was the same in the forest edge habitat. Additionally, species' performance was virtually identical in high light, low light, and transitions between high and low light regimes in the greenhouse. Specific leaf mass of V. nigrum was 17% higher in the common garden and 19% higher in the greenhouse compared with V. rossicum. Both invasive Vincetoxicum spp. appear capable of growing within a broad range of light environments and their management should be similar regardless of light environment. Other explanations are required to explain the scarcity of V. nigrum in low light natural areas. C1 [Averill, Kristine M.; DiTommaso, Antonio] Cornell Univ, Sch Integrat Plant Sci, Soil & Crop Sci Sect, Ithaca, NY 14853 USA. [Whitlow, Thomas H.] Cornell Univ, Hort Sect, Sch Integrat Plant Sci, Ithaca, NY 14853 USA. [Milbrath, Lindsey R.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Averill, KM (reprint author), Cornell Univ, Sch Integrat Plant Sci, Soil & Crop Sci Sect, Ithaca, NY 14853 USA. EM kma25@cornell.edu FU U.S. Department of Agriculture (USDA)-Agricultural Research Service [1907-22620-003-01S] FX The authors thank Scott Morris, Andrew Ebanks, Melanie Ho, and Johnny Means for valuable field and laboratory assistance. Matt Ryan, Tyler Wagner, Emily Rauschert, Stephane Cordeau, and members of the Landscape Ecology at Penn State group provided valuable feedback on earlier manuscript drafts. The authors thank The Nature Conservancy for permission to conduct research in the Great Gully Nature Preserve. Additionally, three anonymous reviewers provided valuable input on previous versions of this article. Financial support was provided by the U.S. Department of Agriculture (USDA)-Agricultural Research Service project number 1907-22620-003-01S. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer. NR 38 TC 0 Z9 0 U1 3 U2 3 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 EI 1939-747X J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD JUL-SEP PY 2016 VL 9 IS 3 BP 171 EP 181 DI 10.1614/IPSM-D-16-00015.1 PG 11 WC Plant Sciences SC Plant Sciences GA EA9EF UT WOS:000386943400002 ER PT J AU Fenesi, A Saura-Mas, S Blank, RR Kozma, A Lozer, BM Ruprecht, E AF Fenesi, Annamaria Saura-Mas, Sandra Blank, Robert R. Kozma, Anita Lozer, Beata-Magdolna Ruprecht, Eszter TI Enhanced Fire-Related Traits May Contribute to the Invasiveness of Downy Brome (Bromus tectorum) SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Cheatgrass; germination; heat shock; ignitability; invasion ID WESTERN NORTH-AMERICA; GREAT-BASIN; GENETIC-VARIATION; SEED-GERMINATION; ALIEN PLANTS; BIOLOGICAL INVASIONS; LITTER FLAMMABILITY; ADAPTIVE EVOLUTION; TEMPERATE FOREST; LEAF TRAITS AB Although several invasive species have induced changes to the fire regime of invaded communities, potential intraspecific shifts in fire-related traits that might enhance the invasion success of these species have never been addressed. We assumed that traits conferring persistence and competitiveness in postfire conditions to downy brome, a quintessential invasive species of the Great Basin (North America), might be under selection in areas with recurrent fires. Therefore, we hypothesized that populations from frequently burned regions of the Great Basin would have (1) greater tolerance to fire at seed level, (2) higher relative seedling performance in postfire environments, and (3) greater flammability than unburned Central European populations that evolved without fire. Seeds were collected from three introduced populations from frequently burned regions in North America and three introduced populations of rarely or never burned sites from Central Europe. We performed (1) germination experiments with seeds subjected to the effect of different fire components (heat shocks, smoke, flame, ash), (2) pot experiments analyzing the effect of postfire conditions on the early growth of the seedlings, and (3) a series of flammability tests on dry biomass of plants reared in a common garden. All seeds tolerated the low-temperature treatments (40 to 100 C), but were destroyed at high heat shocks (140 and 160 C). Only the 100 C heat treatment caused a difference in reaction of seeds from different continents, as the European seeds were less tolerant to this heat shock. We found significantly increased seedling height and biomass after 4 wk of growth under postfire conditions in American populations, but not in European ones. American populations had enhanced flammability in three out of five measured parameters compared to European populations. In summary, these intraspecific differences in fire-related traits might contribute to the persistence and perhaps invasiveness of the frequently burned North American downy brome populations. C1 [Fenesi, Annamaria; Kozma, Anita; Lozer, Beata-Magdolna; Ruprecht, Eszter] Univ Babes Bolyai, Hungarian Dept Biol & Ecol, Republicii St 42, RO-400015 Cluj Napoca, Romania. [Saura-Mas, Sandra] Autonomous Univ Barcelona, Ctr Ecol Res & Forestry Applicat, E-08193 Barcelona, Spain. [Saura-Mas, Sandra] Autonomous Univ Barcelona, Unit Ecol, Dept Anim & Plant Biol & Ecol, E-08193 Barcelona, Spain. [Blank, Robert R.] USDA ARS, Great Basin Rangelands Res Unit, 920 Valley Rd, Reno, NV 89512 USA. RP Fenesi, A (reprint author), Univ Babes Bolyai, Hungarian Dept Biol & Ecol, Republicii St 42, RO-400015 Cluj Napoca, Romania. EM fenesi.annamaria@gmail.com FU European Union; State of Hungary; European Social Fund [TAMOP 4.2.4. A/2-11-1-2012-0001]; CNCS-UEFISCDI [PN-II-RU-TE-2014-4-0381]; grant of Romanian National Authority for Scientific Research and Innovation FX The authors would like to thank Julianna Gered and Eniko Fodor for excellent technical assistance, and Charlie Clements, Dan Harmon (Agricultural Research Service-Great Basin Rangelands Research Unit, Reno NV), Csongor I. Vagasi, and Agnes Albert for supplying seeds of downy brome. A. Fenesi was supported by the European Union and the State of Hungary, cofinanced by the European Social Fund in the framework of TAMOP 4.2.4. A/2-11-1-2012-0001 National Excellence Program. This work was supported by a grant of the Romanian National Authority for Scientific Research and Innovation, CNCS-UEFISCDI, project number PN-II-RU-TE-2014-4-0381. NR 79 TC 0 Z9 0 U1 11 U2 11 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 EI 1939-747X J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD JUL-SEP PY 2016 VL 9 IS 3 BP 182 EP 194 DI 10.1614/IPSM-D-16-00006.1 PG 13 WC Plant Sciences SC Plant Sciences GA EA9EF UT WOS:000386943400003 ER PT J AU Rayamajhi, MB Pratt, PD Tipping, PW Lake, E Smith, M Rohrig, E Dray, FA Center, TD AF Rayamajhi, Min B. Pratt, Paul D. Tipping, Philip W. Lake, Ellen Smith, Melissa Rohrig, Eric Dray, F. Allen Center, Ted D. TI Seasonal Growth, Biomass Allocation, and Invasive Attributes Manifested by Dioscorea bulbifera L. (Air-Potato) Plants Generated from Bulbils in Florida SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Biomass production; common garden study; growth rate; invasive attributes; morphotypes; tuber ID COLEOPTERA-CHRYSOMELIDAE; HARDWOOD FORESTS; OPPOSITIFOLIA AB Dioscorea bulbifera is a serious invader of various ecosystems in Florida, where plants generated by its two morphotypes climb aggressively and smother supporting vegetation. There is a dearth of published research on its invasive biological attributes including vine growth and biomass production by plants generated from bulbils. Herein, we assessed these parameters in common garden studies by planting bulbils from four biomass categories (PBBCs I-IV) of both morphotypes. Vine lengths, longevity-based growth rates (VLGR), biomass, and quantities of leaves and daughter bulbils in both morphotypes showed positive correlation with the biomass of planted parental bulbils. This indicated similarity between corresponding attributes in two morphotypes. Total vine length showed strong positive correlation with VLGR, biomass, and quantities of leaves and bulbils. Overall vine longevity among plants from PBBCs I-IV did not significantly differ whereas the total vine lengths, VLGRs, number of branches, and quantities of leaves and bulbils increased with the biomass of the parental bulbils. Plants recruited by smaller bulbils allocated more biomass to leaves and tubers compared to stems and bulbils, whereas the plants recruited by larger bulbils allocated more biomass to leaves and bulbils compared to tubers and stems. Higher proportion of biomass allocation to leaves and bulbils presumably ensures immediate faster growth, longer vines, and a greater number of daughter bulbils for future recruitment of new plants. Vine length (associated with faster growth rate, capable of blanketing supporting structures and producing large quantities of bulbils) has been noted as the primary invasive biological attribute that facilitates D. bulbifera's status as a noxious exotic weed in Florida. Control measure that can reduce vine length should reduce or eliminate the invasive behavior of D. bulbifera in Florida. C1 [Rayamajhi, Min B.; Tipping, Philip W.; Lake, Ellen; Smith, Melissa; Dray, F. Allen; Center, Ted D.] USDA ARS, Invas Plant Res Lab, Ft Lauderdale, FL 33314 USA. [Pratt, Paul D.] USDA ARS, Western Reg Res Ctr, Exot & Invas Weeds Res Unit, 800 Buchanan St,Room 2136, Albany, CA 94710 USA. [Dray, F. Allen] Florida State Dept Agr, Methods Dev & Biol Control, 1911 SW 34th St, Gainesville, FL 32608 USA. RP Rayamajhi, MB (reprint author), USDA ARS, Invas Plant Res Lab, Ft Lauderdale, FL 33314 USA. EM min.rayamajhi@ars.usda.gov FU Dade County Department of Environmental Resource Management FX We acknowledge the assistance of Jorge Leidi, Reynaldo Moscat (USDA/ARS, Invasive Plant Research Laboratory, Fort Lauderdale, FL, and many student interns from Miami Dade and Broward colleges. Thanks are also due to Dade County Department of Environmental Resource Management for providing partial funding for this project. NR 34 TC 0 Z9 0 U1 2 U2 2 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 EI 1939-747X J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD JUL-SEP PY 2016 VL 9 IS 3 BP 195 EP 204 DI 10.1614/IPSM-D-16-00022.1 PG 10 WC Plant Sciences SC Plant Sciences GA EA9EF UT WOS:000386943400004 ER PT J AU Ortiz-Santana, B Bessette, AE McConnell, OL AF Ortiz-Santana, Beatriz Bessette, Alan E. McConnell, Owen L. TI Boletus durhamensis sp nov from North Carolina SO MYCOTAXON LA English DT Article DE Boletaceae; ectomycorrhizal fungi; oaks; taxonomy ID IDENTIFICATION; APPENDICULATI; BUTYRIBOLETUS; GENUS AB A new bolete with cinnamon-brown pores, Boletus durhamensis, is described. Collected in northern North Carolina, it is possibly mycorrhizal with Quercus spp. Morphological and molecular characters support this taxon as a new species. C1 [Ortiz-Santana, Beatriz] US Forest Serv, Northern Res Stn, Ctr Forest Mycol Res, One Gifford Pinchot Dr, Madison, WI 53726 USA. [Bessette, Alan E.] 2170 Live Oak Circle, St Marys, GA 31558 USA. [McConnell, Owen L.] 2808 Butner St, Durham, NC 27704 USA. RP Ortiz-Santana, B (reprint author), US Forest Serv, Northern Res Stn, Ctr Forest Mycol Res, One Gifford Pinchot Dr, Madison, WI 53726 USA. EM bortizsantana@fs.fed.us NR 31 TC 0 Z9 0 U1 2 U2 2 PU MYCOTAXON LTD PI ITHACA PA PO BOX 264, ITHACA, NY 14851-0264 USA SN 0093-4666 J9 MYCOTAXON JI Mycotaxon PD JUL-SEP PY 2016 VL 131 IS 3 BP 703 EP 715 DI 10.5248/131.703 PG 13 WC Mycology SC Mycology GA EA4PH UT WOS:000386594600028 ER PT J AU Wijewardane, NK Ge, YF Wills, S Loecke, T AF Wijewardane, Nuwan K. Ge, Yufeng Wills, Skye Loecke, Terry TI Prediction of Soil Carbon in the Conterminous United States: Visible and Near Infrared Reflectance Spectroscopy Analysis of the Rapid Carbon Assessment Project SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID ORGANIC-CARBON; NIR SPECTROSCOPY; SPECTRAL LIBRARIES; REGRESSION; MOISTURE; DATABASE; STOCKS AB Visible and near infrared reflectance spectroscopy (VisNIR) has been used across a number of spatial scales to predict soil organic carbon (OC) content. The Rapid Carbon Assessment Project (RaCA) is a nationwide project that collected 144,000+ soil samples from across the conterminous United States for C stock mapping using VisNIR. The objective of this study was to calibrate and validate the VisNIR soil OC and total C (TC) models with -20,000 samples from RaCA. Models were developed with either partial least squares regression (PLSR) or the Artificial Neural Network (ANN) model. Four auxiliary variables-RaCA Region, Land Use Land Cover, Master Horizon, and Textural Class-were tested to stratify the dataset and to develop local models. The results showed that OC and TC models calibrated with ANN (R-2 > 0.94; RPD > 4.0) outperformed those of PLSR (R-2 = 0.83; RPD = 2.4). For PLSR, local models developed with all four auxiliary variables exhibited improvement in prediction accuracy, and the improvement was only marginal for ANN models. Master Horizon and Textural Class appeared to be more effective in stratifying samples into homogeneous groups because they gave an overall lower root mean squared error of prediction (RMSEP) for the validation samples. For the majority of the local Textural Class models, the RMSEP of OC prediction ranged from 0.5 to 1.5%. To maximize the applicability of the RaCA spectral library on external soil samples, PLSR local models developed from Master Horizon or Textural Class appeared to be more favorable. C1 [Wijewardane, Nuwan K.; Ge, Yufeng] Univ Nebraska, Dept Biol Syst Engn, Lincoln, NE 68583 USA. [Wills, Skye] Natl Soil Survey Ctr, Nat Resource Conservat Serv, USDA, 100 Centennial Mall, Lincoln, NE 68508 USA. [Loecke, Terry] Univ Kansas, Environm Studies Program, Lawrence, KS 66045 USA. RP Ge, YF (reprint author), Univ Nebraska, Dept Biol Syst Engn, Lincoln, NE 68583 USA. EM yge2@unl.edu FU USDA-NRCS FX Funding for this project was provided by the USDA-NRCS. The authors thank the staff at the Kellogg Soil Survey Lab of the USDA-NRCS for maintaining and querying the RaCA database for this study. NR 42 TC 0 Z9 0 U1 5 U2 5 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD JUL-AUG PY 2016 VL 80 IS 4 BP 973 EP 982 DI 10.2136/sssaj2016.02.0052 PG 10 WC Soil Science SC Agriculture GA EA2YJ UT WOS:000386463000015 ER PT J AU Halvorson, JJ Liebig, MA Archer, DW West, MS Tanaka, DL AF Halvorson, Jonathan J. Liebig, Mark A. Archer, David W. West, Mark S. Tanaka, Donald L. TI Impacts of Crop Sequence and Tillage Management on Soil Carbon Stocks in South-Central North Dakota SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID LONG-TERM TILLAGE; GREAT-PLAINS; ORGANIC-MATTER; ECOSYSTEM SERVICES; CHEMICAL-PROPERTIES; LIVESTOCK SYSTEMS; PRECIPITATION USE; WHEAT-FALLOW; DRYLAND SOIL; QUALITY AB Emphasis is placed on developing robust, productive, economically competitive, and environmentally benign agroecosystems. In 2012, after 18 yr of a study to evaluate the effects of crop sequence and tillage, we measured soil properties at various depths to 91.4 cm and estimated soil C. The experiment, near Mandan, ND, was a split-plot design, with crop sequence as whole plots and tillage (minimum tillage or no-till) as subplots. Crop sequences included continuous spring wheat (Triticum aestivum L.) with crop residue retained or removed, spring wheat-millet [Setaria italica (L.) Beauv.], spring wheat-safflower (Carthamus tinctorius L.)-fallow, spring wheat-safflower-rye (Secale cereale L.), and spring wheat-fallow. Soil organic C, calculated by the equivalent mass method (SOCem), was consistently higher (P < 0.001) than when calculated by the fixed-depth method. Disparities between the two methods, greatest near the soil surface, were attributable to tillage. Estimates of SOCem were not affected by cropping sequence at any depth, averaging about 26, 47, 73, 114, and 156 Mg ha(-1) in the 0- to 7.6-, 0- to 15.2-, 0- to 30.5-, 0- to 61.0-, and 0- to 91.4-cm partitions. Tillage had no influence at depths < 30.5 cm, but no-till plots contained more SOCem than minimum tillage plots in the 0- to 61-cm (115 vs. 112 Mg ha(-1)) and 0- to 91.4-cm (158 vs. 153 2.3 Mg ha(-1)) partitions (P < 0.05). Soil organic C in the 0- to 7.6-cm depth increased by an average of 3.2 Mg ha(-1) or 16% since 2001. This study suggests that C sequestration in soil is less impacted by the choice of crop sequence than by tillage. C1 [Halvorson, Jonathan J.; Liebig, Mark A.; Archer, David W.; Tanaka, Donald L.] USDA ARS, Northern Great Plains Res Lab, Mandan, ND 58554 USA. [West, Mark S.] USDA ARS, Rangeland Resources Res Unit, Northern Plains Area, Ft Collins, CO 80526 USA. RP Halvorson, JJ (reprint author), USDA ARS, Northern Great Plains Res Lab, Mandan, ND 58554 USA. EM Jonathan.Halvorson@ars.usda.gov NR 57 TC 0 Z9 0 U1 4 U2 4 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD JUL-AUG PY 2016 VL 80 IS 4 BP 1003 EP 1010 DI 10.2136/sssaj2015.09.0331 PG 8 WC Soil Science SC Agriculture GA EA2YJ UT WOS:000386463000018 ER PT J AU Blanco-Canqui, H Tatarko, J Stalker, AL Shaver, TM van Donk, SJ AF Blanco-Canqui, Humberto Tatarko, John Stalker, Aaron L. Shaver, Tim M. van Donk, Simon J. TI Impacts of Corn Residue Grazing and Baling on Wind Erosion Potential in a Semiarid Environment SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID PREDICTION SYSTEM WEPS; SIMULATED SOIL LOSSES; FREEZE-THAW CYCLES; ORGANIC-MATTER; AGGREGATE STABILITY; STOVER REMOVAL; WATER CONTENT; STOCKING RATE; VALIDATION; SWEEP AB Implications of corn (Zea mays L.) residue grazing and baling on wind erosion in integrated crop-livestock systems are not well understood. This study (i) determined soil properties affecting wind erosion potential including dry aggregate-size distribution, geometric mean diameter (GMD), geometric standard deviation of dry aggregates, and wind-erodible fraction (WEF), (ii) correlated these properties with soil organic C (SOC) and particulate organic matter (POM), and (iii) simulated soil loss using the Single-event Wind Erosion Evaluation Program (SWEEP) model after 7 and 8 yr of irrigated no-till corn residue management in a semiarid region in west-central Nebraska. Residue treatments were: control (no residue removal), light grazing (2.5 animal unit months [AUM] ha(-1)), heavy grazing (5.0 AUM ha(-1)), and baling. We simulated soil loss for a 3-h windstorm with a wind velocity of 13 m s(-1). Soil properties differed in spring but not in fall. Baling reduced 6.3 to 45-mm macroaggregates by 37% and GMD by 80% and increased WEF by 25% relative to the control. Light and heavy grazing, after 8 yr, significantly reduced 6.3- to 14-mm macroaggregates 43% compared with the control and tended to reduce GMD and increase WEF, although not statistically significant. As residue cover decreased, GMD decreased and WEF increased. Residue removal did not reduce SOC and POM concentrations, but soil erodibility decreased as POM increased. Simulation showed that soil erodibility increased as residue cover decreased in spring, and baling increased the wind erosion potential. Overall, residue baling increases the wind erosion potential but residue grazing has smaller effects in this semiarid environment. C1 [Blanco-Canqui, Humberto] Univ Nebraska Lincoln, Dept Agron & Hort, 261 Plant Sci Hall, Lincoln, NE 68583 USA. [Tatarko, John] USDA ARS, Agr Syst Res Unit, Ft Collins, CO 80526 USA. [Stalker, Aaron L.; Shaver, Tim M.] Univ Nebraska, West Cent Res & Extens Ctr, North Platte, NE 69101 USA. [van Donk, Simon J.] Iteris Inc, Grand Forks, ND 58203 USA. RP Blanco-Canqui, H (reprint author), Univ Nebraska Lincoln, Dept Agron & Hort, 261 Plant Sci Hall, Lincoln, NE 68583 USA. EM hblanco2@unl.edu NR 35 TC 0 Z9 0 U1 10 U2 10 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD JUL-AUG PY 2016 VL 80 IS 4 BP 1027 EP 1037 DI 10.2136/sssaj2016.03.0073 PG 11 WC Soil Science SC Agriculture GA EA2YJ UT WOS:000386463000021 ER PT J AU Johnson, JMF Barbour, NW AF Johnson, Jane M. F. Barbour, Nancy W. TI Nitrous Oxide Emission and Soil Carbon Sequestration from Herbaceous Perennial Biofuel Feedstocks SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID WARM-SEASON GRASSES; GROWING-SEASON; ORGANIC-CARBON; BIG BLUESTEM; CORN-BELT; SWITCHGRASS; BIOMASS; MANAGEMENT; HARVEST; YIELD AB Switchgrass (Panicum virgatum L.) and big bluestem (Andropogon gerdardii Vitman) are potential bioenergy feedstocks. Perennial grasses managed as bioenergy feedstock require nitrogenous inputs, which can cause N2O emission and, thereby, alter their effectiveness to mitigate greenhouse gas (GHG) emission. Few studies have measured N2O emission from perennial grasses managed as feedstock. The objectives of this study were to compare N2O flux and soil organic C (SOC) storage between (i) grasses with legume companion crops or with nitrogenous fertilizer, (ii) two grass harvest times (autumn and spring), and (iii) perennial systems and corn (Zea maize L.)-soybean [Glycine max (L.) Merr.] (C-S) rotation, all without tillage. Nitrous oxide flux was measured from May 2009 to May 2012, and SOC was measured in 2000, 2006, and 2011. Big bluestem-clover (Dalea) and switchgrass-clover treatments had dramatically reduced annual N2O emissions and yield-scaled emissions compared with the respective grasses with urea fertilizer. Cumulative N2O emission was 14 to 40% greater in the big bluestem-spring and switchgrass-spring treatments compared with respective autumn-harvested treatments. The average cumulative emission in fertilized big bluestem and switchgrass treatments (18.5 kg N2O-N ha(-1)) exceeded that of the C-S rotation (12.7 kg N2O-N ha(-1)). The emission factor (EF) for fertilized grasses averaged 2.5%, corn averaged 1.05%, and C-S rotation averaged 1.9%. The SOC storage by perennial grasses was limited to 0 to 5 cm and thus may not be adequate to offset N2O emission. Nitrogen management refinement is recommended for herbaceous perennials to optimize biomass production and minimize N2O emission. C1 [Johnson, Jane M. F.; Barbour, Nancy W.] USDA ARS, North Cent Soil Conservat Res Lab, 803 Iowa Ave, Morris, MN 56267 USA. RP Johnson, JMF (reprint author), USDA ARS, North Cent Soil Conservat Res Lab, 803 Iowa Ave, Morris, MN 56267 USA. EM Jane.Johnson@ars.usda.gov OI Johnson, Jane/0000-0002-1687-4007 FU USDA-Agricultural Research Service GRACEnet project; USDA-Agricultural Research Service REAP project FX We gratefully acknowledge Dr. Don Reicosky (retired ARS Scientist) for his forethought in establishing these long-term plots. The authors thank G. Amundson, B. Knicke, and C. Rollofson for their assistance and analytical support collecting, processing, and analyzing gas, soil, and plant samples; J. Hanson for his analytical support in the chemical analyses of soil and plant samples; C. Wente, C. Hennen, and S. Larson for their assistance in plot maintenance; J. Zaharick for proof-reading the revised manuscript; and the reviewers and Associate Editor for their careful, thoughtful, and courteous reviews. This publication is based on research supported by the USDA-Agricultural Research Service GRACEnet and REAP projects. NR 61 TC 1 Z9 1 U1 3 U2 3 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD JUL-AUG PY 2016 VL 80 IS 4 BP 1057 EP 1070 DI 10.2136/sssaj2015.12.0436 PG 14 WC Soil Science SC Agriculture GA EA2YJ UT WOS:000386463000023 ER PT J AU Rodriguez, JB Self, JR Arriaga, FJ AF Rodriguez, Juan B. Self, James R. Arriaga, Francisco J. TI A Simple, Gravimetric Method to Quantify Inorganic Carbon in Calcareous Soils SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article AB Total C (TC) in calcareous soils has two components: inorganic C (IC) and organic C (OC). The IC must be measured and subtracted from the TC to obtain OC. Our objective was to develop a simple gravimetric technique to quantify IC. This technique, named the carbonate meter (CM), can be assembled in any soil testing laboratory with a 6-mL glass vial, a no. 1 rubber stopper with one hole, a 6.5-cm glass tube with 0.5- and 0.3-cm outside and inside diameters, respectively, and a 50-mL Erlenmeyer flask. Precision and recovery was tested with 0.728 and 2.57% IC standard calcareous soils and pure calcite and dolomite, using 0.2000 +/- 0.0200 g of finely ground samples and 4.0 mL of fresh 4 mol L-1 HCl-3% FeCl2 center dot 4H(2)O. Twelve US calcareous soils with 0.11 to 8.52% IC were used to validate our CM procedure. Inorganic C recoveries after 22 to 24 h for two standard calcareous soils, calcite, and dolomite were 100.55, 99.73, 100.45, and 99.58%, respectively. The detection limit (DL) and the lower limit of quantitation (LLOQ) for calcite were 0.31 and 1.0 g IC kg(-1), respectively. The DL and the LLOQ for dolomite were 0.42 and 1.4 g IC kg(-1), respectively. Mean IC recovery with a modified pressure calcimeter (MPC) in reference standards was 56.42%, while the CM recovery was 100.84%. The MPC loses accuracy in calcareous soils with >5.11% IC, whereas the CM had good recoveries up to 8.40% IC. The CM does not require pre-analysis, it is easy, non-tedious, and inexpensive, with good precision and accuracy. C1 [Rodriguez, Juan B.; Arriaga, Francisco J.] USDA ARS, Natl Soil Dynam Lab, 411 South Donahue Dr, Auburn, AL 36832 USA. [Self, James R.] Colorado State Univ, Soil Water & Plant Testing Lab, 200 West Lake St NESB, Ft Collins, CO 80523 USA. RP Rodriguez, JB (reprint author), USDA ARS, Natl Soil Dynam Lab, 411 South Donahue Dr, Auburn, AL 36832 USA. EM juan.rodriguez@ars.usda.gov NR 18 TC 0 Z9 0 U1 3 U2 3 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD JUL-AUG PY 2016 VL 80 IS 4 BP 1107 EP 1113 DI 10.2136/sssaj2015.09.0355 PG 7 WC Soil Science SC Agriculture GA EA2YJ UT WOS:000386463000028 ER PT J AU Kim, S AF Kim, Sanghoon TI A Protocol for the Production of Gliadin-cyanoacrylate Nanoparticles for Hydrophilic Coating SO JOVE-JOURNAL OF VISUALIZED EXPERIMENTS LA English DT Article DE Chemistry; Issue 113; Nanoparticles; Cyanoacrylate; Gliadin; Wetting; Hydrophilic coating; Adsorption AB This article presents a protocol for the production of protein-based nanoparticles that changes the hydrophobic surface to hydrophilic by a simple spray coating. These nanoparticles are produced by the polymerization reaction of alkyl cyanoacrylate on the surface of cereal protein (gliadin) molecules. Alkyl cyanoacrylate is a monomer that instantly polymerizes at RT when it is applied to the surface of materials. Its polymerization reaction is initiated by the trace amounts of weakly basic or nucleophilic species on the surface, including moisture. Once polymerized, the polymerized alkyl cyanoacrylates show a strong affinity with the object materials because nitrile groups are in the backbone of poly (alkyl cyanoacrylate). Proteins also work as initiator for this polymerization because they contain amine groups that can initiate the polymerization of cyanoacrylate. If aggregated protein is used as an initiator, protein aggregate is surrounded by the hydrophobic poly(alkyl cyanoacrylate) chains after the polymerization reaction of alkyl cyanoacrylate. By controlling the experimental condition, particles in the nanometer range are produced. The produced nanoparticles readily adsorb to the surface of most materials including glass, metals, plastics, wood, leather, and fabrics. When the surface of a material is sprayed with the produced nanoparticle suspension and rinsed with water, the micellar structure of nanoparticle changes its conformation, and the hydrophilic proteins are exposed to the air. As a result, the nanoparticle-coated surface changes to hydrophilic. C1 [Kim, Sanghoon] USDA ARS NCAUR, Beltsville, MD 20705 USA. RP Kim, S (reprint author), USDA ARS NCAUR, Beltsville, MD 20705 USA. EM Sanghoon.Kim@ars.usda.gov NR 11 TC 0 Z9 0 U1 2 U2 2 PU JOURNAL OF VISUALIZED EXPERIMENTS PI CAMBRIDGE PA 1 ALEWIFE CENTER, STE 200, CAMBRIDGE, MA 02140 USA SN 1940-087X J9 JOVE-J VIS EXP JI J. Vis. Exp. PD JUL PY 2016 IS 113 AR e54147 DI 10.3791/54147 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ1TG UT WOS:000385622600048 ER PT J AU Viger, M Smith, HK Cohen, D Dewoody, J Trewin, H Steenackers, M Bastien, C Taylor, G AF Viger, Maud Smith, Hazel K. Cohen, David Dewoody, Jennifer Trewin, Harriet Steenackers, Marijke Bastien, Catherine Taylor, Gail TI Adaptive mechanisms and genomic plasticity for drought tolerance identified in European black poplar (Populus nigra L.) SO TREE PHYSIOLOGY LA English DT Article DE carbon isotope discrimination (Delta C-13); microarray; stomatal number; water deficit ID WATER-USE EFFICIENCY; CARBON-ISOTOPE DISCRIMINATION; STOMATAL DEVELOPMENT; CLIMATE-CHANGE; TRANSPIRATION EFFICIENCY; PHYSIOLOGICAL TRAITS; RICE GENOTYPES; LEAF TRAITS; STRESS; CLONES AB Summer droughts are likely to increase in frequency and intensity across Europe, yet long-lived trees may have a limited ability to tolerate drought. It is therefore critical that we improve our understanding of phenotypic plasticity to drought in natural populations for ecologically and economically important trees such as Populus nigra L. A common garden experiment was conducted using similar to 500 wild P. nigra trees, collected from 11 river populations across Europe. Phenotypic variation was found across the collection, with southern genotypes from Spain and France characterized by small leaves and limited biomass production. To examine the relationship between phenotypic variation and drought tolerance, six genotypes with contrasting leaf morphologies were subjected to a water deficit experiment. 'North eastern' genotypes were collected at wet sites and responded to water deficit with reduced biomass growth, slow stomatal closure and reduced water use efficiency (WUE) assessed by Delta C-13. In contrast, 'southern' genotypes originating from arid sites showed rapid stomatal closure, improved WUE and limited leaf loss. Transcriptome analyses of a genotype from Spain (Sp2, originating from an arid site) and another from northern Italy (Ita, originating from a wet site) revealed dramatic differences in gene expression response to water deficit. Transcripts controlling leaf development and stomatal patterning, including SPCH, ANT, ER, AS1, AS2, PHB, CLV1, ERL1-3 and TMM, were down-regulated in Ita but not in Sp2 in response to drought. C1 [Viger, Maud; Smith, Hazel K.; Dewoody, Jennifer; Trewin, Harriet; Taylor, Gail] Univ Southampton, Ctr Biol Sci, Life Sci Bldg, Southampton SO17 1BJ, Hants, England. [Cohen, David] INRA NANCY Lorraine, UMR Ecol & Ecophysiol Forestieres, F-54280 Champenoux, France. [Cohen, David] Univ Lorraine, UMR Ecol & Ecophysiol Forestiere, BP 239, F-54506 Vandoeuvre Les Nancy, France. [Steenackers, Marijke] Res Inst Nat & Forest INBO, Minist Flemish Community, B-9500 Geraardsbergen, Belgium. [Bastien, Catherine] INRA, Unite Rech Ameliorat Genet & Physiol Forestieres, 2163 Ave Pomme Pin,CS 40001 Ardon, F-45075 Orleans 2, France. [Dewoody, Jennifer] US Forest Serv, USDA, Natl Forest Genet Lab, 2480 Carson Rd, Placerville, CA 95667 USA. RP Taylor, G (reprint author), Univ Southampton, Ctr Biol Sci, Life Sci Bldg, Southampton SO17 1BJ, Hants, England. EM g.taylor@soton.ac.uk FU European Commission through the Directorate General Research within the Fifth Framework for Research - Quality of Life and Management of the Living Resources Programme [QLK5-CT-2002-00953]; European Commission through the Directorate General Research within the Sixth Framework for Research as part of the Network of Excellence EVOLTREE [016322]; European Commission through the Directorate General Research within Global Change and Ecosystems Programme; European Commission through Seventh Framework for Research, Food Agriculture and Fisheries, Biotechnology, within the project ENERGYPOPLAR [FP7-211917]; European Commission through Seventh Framework for Research, Food Agriculture and Fisheries, Biotechnology, within WATBIO [FP7-311929]; NERC; RCUK FX This research was supported by the European Commission through the Directorate General Research within the Fifth Framework for Research - Quality of Life and Management of the Living Resources Programme, contact No. QLK5-CT-2002-00953 (POPYOMICs), within the Sixth Framework for Research as part of the Network of Excellence EVOLTREE, contract No. 016322 - Sustainable development, Global Change and Ecosystems Programme and through the Seventh Framework for Research, Food Agriculture and Fisheries, Biotechnology, within the project ENERGYPOPLAR, contract No. FP7-211917 and WATBIO, contract No. FP7-311929. H.T. was supported by a PhD studentship from NERC. Funding to pay the Open Access publication charges for this article was provided by RCUK. NR 75 TC 1 Z9 1 U1 11 U2 11 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0829-318X EI 1758-4469 J9 TREE PHYSIOL JI Tree Physiol. PD JUL PY 2016 VL 36 IS 7 BP 909 EP 928 DI 10.1093/treephys/tpw017 PG 20 WC Forestry SC Forestry GA DY7DX UT WOS:000385290900010 PM 27174702 ER PT J AU Bramwell, L AF Bramwell, Lincoln TI Hail of Fire: A Man and His Family Face Natural Disaster SO ENVIRONMENTAL HISTORY LA English DT Book Review C1 [Bramwell, Lincoln] US Forest Serv, USDA, Washington, DC 20250 USA. RP Bramwell, L (reprint author), US Forest Serv, USDA, Washington, DC 20250 USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1084-5453 EI 1930-8892 J9 ENVIRON HIST-US JI Environ. Hist. PD JUL PY 2016 VL 21 IS 3 BP 559 EP 561 DI 10.1093/envhis/emw025 PG 4 WC Environmental Studies; History SC Environmental Sciences & Ecology; History GA DY2KE UT WOS:000384920700012 ER PT J AU Liu, NT Bauchan, GR Francoeur, CB Shelton, DR Lo, YM Nou, XW AF Liu, Nancy T. Bauchan, Gary R. Francoeur, Charlotte B. Shelton, Daniel R. Lo, Y. Martin Nou, Xiangwu TI Ralstonia insidiosa serves as bridges in biofilm formation by foodborne pathogens Listeria monocytogenes, Salmonella enterica, and Enterohemorrhagic Escherichia coli SO FOOD CONTROL LA English DT Article DE Ralstonia insidiosa; Bridging bacteria; Dual-species biofilms; Foodborne pathogens ID DUAL-SPECIES BIOFILMS; DRINKING-WATER; O157H7; MICROORGANISMS; ACID; COAGGREGATION; RESISTANCE; PICKETTII; SURFACES; BACTERIA AB Biofilm formation on abiotic surfaces in fresh produce processing facilities may play a role in foodborne outbreaks by providing protective microniches for pathogenic bacteria. Our previous study showed that a strain of Ralstonia insidiosa isolated from a fresh produce processing plant could enhance the incorporation of Escherichia coli O15:H7 in biofilms under various environmental conditions. These results raised the concern that R. insidiosa might have the ability to incorporate other foodborne pathogens and promote their survival and growth in biofilms. To test this hypothesis, 6 strains of Shiga toxin producing E. coli, 2 strains of Salmonella, and 6 strains of Listeria monocytogenes were examined for dual-species biofilm formation with R. insidiosa. A significant increase in biomass formation was observed in 7 of the 14 R. insidiosa-pathogen combinations, while significantly enhanced incorporation of pathogenic cells into biofilms was seen in 12 of the 14 R. insidiosa-pathogen combinations. The synergistic interactions between R. insidiosa and the tested foodborne pathogens seemed dependent on intimate cellular contact between the two strains. Overall, this study showed that R. insidiosa could enhance the incorporation of biofilms of different types of foodborne pathogenic bacteria and should be considered a bridging bacterium for biofilm formation in various food processing environments. Published by Elsevier Ltd. C1 [Liu, Nancy T.; Francoeur, Charlotte B.; Shelton, Daniel R.; Nou, Xiangwu] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Bauchan, Gary R.] ARS, Electron & Confocal Microscopy Res Unit, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Liu, Nancy T.; Lo, Y. Martin] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20740 USA. [Francoeur, Charlotte B.] Univ Maryland, Dept Cell Biol & Mol Genet, College Pk, MD 20740 USA. RP Nou, XW (reprint author), ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. EM xiangwu.nou@ars.usda.gov NR 31 TC 1 Z9 1 U1 3 U2 4 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD JUL PY 2016 VL 65 BP 14 EP 20 DI 10.1016/j.foodcont.2016.01.004 PG 7 WC Food Science & Technology SC Food Science & Technology GA DX8GQ UT WOS:000384626500003 ER PT J AU Roberts, AJ Funston, RN Grings, EE Petersen, MK AF Roberts, A. J. Funston, R. N. Grings, E. E. Petersen, M. K. TI TRIENNIAL REPRODUCTION SYMPOSIUM: Beef heifer development and lifetime productivity in rangeland-based production systems SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE fetal programing; lifetime productivity; nutritional supplementation ID MATERNAL UNDERNUTRITION; NUTRITIONAL ASPECTS; CALF PERFORMANCE; BODY CONDITION; COWS; GROWTH; WEIGHT; RESTRICTION; EPIGENETICS; CATTLE AB Nutritional and environmental factors have been shown to cause epigenetic changes that influence characteristics of the offspring throughout life. In livestock, small differences in nutrition during gestation may alter lifetime production efficiency of offspring. Therefore, the potential for fetal programing should be considered when determining supplemental feeding strategies during gestation. For example, female offspring born to cows grazing dormant winter pasture supplemented with 1.1 kg/d of alfalfa hay during the last third of gestation were 10 kg heavier and had greater BCS at 5 yr of age than those from dams supplemented with 1.8 kg/d of alfalfa hay. These differences were beneficial for maintaining reproductive performance in offspring managed with fewer harvested feed inputs. Evaluation of female offspring from cows wintered on either low-quality or high-quality pasture for 30 to 45 d during the fifth to sixth month of gestation indicated a trend for longer duration of productivity in daughters from cows wintered on improved pasture. In recent studies comparing offspring from cows with or without protein supplementation while grazing dormant winter range during late gestation, heifers from protein-supplemented dams had greater BW at weaning. This BW increase persisted throughout pregnancy and to subsequent calving, and pregnancy rates were greater in heifers from protein-supplemented dams. Heifers from protein-supplemented dams had lower G: F compared with heifers from unsupplemented dams. Therefore, in utero exposure to nutritionally limited environments (nonsupplemented dams) may promote greater feed efficiency in the heifer offspring later in life. Nutrition during postweaning development may also affect lifetime productivity. Heifers developed on low-quality native range with RUP supplementation had greater retention beyond 3 yr of age than cohorts developed in a feedlot with higher quality feed and greater ADG. Collectively, these examples show nutritional management strategies used during gestation and development may influence lifetime productivity. C1 [Roberts, A. J.; Petersen, M. K.] ARS, USDA, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. [Funston, R. N.] Univ Nebraska, West Cent Res & Extens Ctr, North Platte, NE 69101 USA. [Grings, E. E.] South Dakota State Univ, Dept Anim Sci, Brookings, SD 57007 USA. [Grings, E. E.] US Agcy Int Dev, Bur Food Secur, Washington, DC 20523 USA. RP Roberts, AJ (reprint author), ARS, USDA, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. EM andy.roberts@ars.usda.gov FU Journal of Animal Science; American Society of Animal Science FX Based on a presentation at the Triennial Reproduction Symposium titled "Developmental programming of fertility" at the 2015 Joint Annual Meeting of the American Dairy Science Association (ADSA) and the American Society of Animal Science (ASAS) held in Orlando, Florida, July 12-16, with publication sponsored by the Journal of Animal Science and the American Society of Animal Science. NR 37 TC 1 Z9 1 U1 6 U2 7 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUL PY 2016 VL 94 IS 7 BP 2705 EP 2715 DI 10.2527/jas2016-0435 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA DY3PE UT WOS:000385005300002 PM 27482658 ER PT J AU Truong, AD Ban, J Park, B Hong, YH Lillehoj, HS AF Truong, A. D. Ban, J. Park, B. Hong, Y. H. Lillehoj, H. S. TI Characterization and functional analyses of a novel chicken CD8 alpha variant X1 (CD8 alpha 1) SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE CD8 alpha variant X1; chicken; cytokines; major histocompatibility complex; signal transduction ID DIFFERENTIAL GENE-EXPRESSION; COLONY-STIMULATING FACTOR; CD8(+) T-CELLS; MHC CLASS-I; SIGNAL-TRANSDUCTION; MOLECULAR-CLONING; NECROTIC ENTERITIS; CRYSTAL-STRUCTURE; IMMUNE-SYSTEM; CD4 AB We provide the first description of cloning and of structural and functional analysis of a novel variant in the chicken cluster of differentiation 8 alpha (CD8 alpha) family, termed the CD8 alpha X1 (CD8 alpha 1) gene. Multiple alignments of CD8a1 with known CD8 alpha and CD8 beta sequences of other species revealed relatively low conservation of AA residues involved in the specific and unique structural domains among CD8 alpha genes. For example, cysteine residues that are involved in disulfide bonding to form the V domain are conserved. In contrast, the O-linked glycosylation sites (XPXX motif) are not found in the chicken CD8 alpha 1 sequence, and the A beta strand and complementarity-determining region 1 and 2 sequences are poorly conserved between chicken CD8 alpha 1 and avian CD8 alpha. Furthermore, the alignment showed that the transmembrane regions show relatively high sequence similarity, whereas the cytoplasmic regions show relatively low similarity, indicating poor conservation. Moreover, the motif (CXCP) that is thought to be responsible for binding the p56 lymphocyte cell kinase subunit (p56(lck)) is missing in the CD8 alpha 1 sequence. The chicken CD8 alpha 1 genomic structure is similar to that of chicken CD8 alpha, but their protein structures differ. Phylogenetic analysis showed that chicken CD8 alpha 1 grouped with known avian CD8 alpha sequences but was somewhat distantly related to the CD8 alpha molecules of other species. Moreover, we analyzed the signal transduction and cytokine response to CD8 alpha 1 treatment to determine the specific biological functions of chicken CD8 alpha 1 in immune cells. The results showed that chicken CD8 alpha 1 is a key regulator of the expression of genes that are associated and cooperate with transcription factors in the major histocompatibility complex class I and II promoter regions and activates Janus kinase (JAK) 1/2, signal transducer and activator of transcription (STAT), and suppressor of cytokine signaling (SOCS) 1 signaling-related genes. Immune cells that express functional CD8 alpha 1 induce proinflammatory cytokines as well as innate immune responses. Therefore, our data indicate that CD8 alpha 1 may have immunoregulatory activity by regulating the expression of proinflammatory or anti-inflammatory cytokines via its effect on immune cells. C1 [Truong, A. D.; Ban, J.; Park, B.; Hong, Y. H.] Chung Ang Univ, Dept Anim Sci & Technol, Anseong 17546, South Korea. [Lillehoj, H. S.] ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD 20705 USA. RP Hong, YH (reprint author), Chung Ang Univ, Dept Anim Sci & Technol, Anseong 17546, South Korea. EM yhong@cau.ac.kr FU Golden Seed Project [PJ009925022016]; Chung-Ang University Excellent Student Scholarship, Republic of Korea FX This work was supported by the Golden Seed Project (PJ009925022016) and the Chung-Ang University Excellent Student Scholarship in 2014, Republic of Korea. NR 59 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUL PY 2016 VL 94 IS 7 BP 2737 EP 2751 DI 10.2527/jas2015-0133 PG 15 WC Agriculture, Dairy & Animal Science SC Agriculture GA DY3PE UT WOS:000385005300005 PM 27482661 ER PT J AU Buntyn, JO Sanchez, NCB Schmidt, TB Erickson, GE Sieren, SE Jones, SJ Carroll, JA AF Buntyn, J. O. Sanchez, N. C. Burdick Schmidt, T. B. Erickson, G. E. Sieren, S. E. Jones, S. J. Carroll, J. A. TI The metabolic, stress axis, and hematology response of zilpaterol hydrochloride supplemented beef heifers when exposed to a dual corticotropin-releasing hormone and vasopressin challenge SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE beta-agonist; cattle; metabolic variables; stress ID CATTLE FOLLOWING TRANSPORTATION; STEERS; PERFORMANCE; QUALITY; GLUCOCORTICOIDS; RELOCATION; AGONISTS; PORK AB The objective of this study was to determine the metabolic, stress, and hematology response of beef heifers supplemented with zilpaterol hydrochloride (ZH) when exposed to an endocrine stress challenge. Heifers (n = 20; 556 +/- 7 kg BW) were randomized into 2 treatment groups: 1) control (CON), no ZH supplementation, and 2) zilpaterol (ZIL), supplemented with ZH at 8.33 mg/kg (DM basis). The ZIL group was supplemented ZH for 20 d, with a 3-d withdrawal period. On d 24, heifers received an intravenous bolus of corticotropin-releasing hormone (CRH; 0.3 mu g/kg BW) and arginine vasopressin (VP; 1.0 mu g/kg BW) to activate the stress axis. Blood samples were collected at 30-min intervals for serum and 60-min intervals for plasma and whole blood, from -2 to 8 h relative to the challenge at 0 h (1000 h). Samples were analyzed for glucose, insulin, NEFA, blood urea nitrogen (BUN), cortisol, epinephrine, norepinephrine, and complete blood cell counts. Following the challenge, cattle were harvested over a 3-d period. Liver, LM, and biceps femoris (BF) samples were collected and analyzed for glucose, lactate, and glycolytic potential (GP). There was a treatment (P = 0.001) effect for vaginal temperature (VT), with ZIL having a 0.1 degrees C decrease in VT when compared with CON. A treatment x time effect (P = 0.002) was observed for NEFA. A treatment effect was observed for BUN; ZIL had decreased BUN concentrations compared with CON (P < 0.001) prior to the challenge; however, no treatment x time effect was observed. There was also a treatment effect for cortisol (P <= 0.01) and epinephrine (P = 0.003); ZIL had decreased cortisol and epinephrine during the CRH/VP challenge when compared with CON. There was a time effect for total white blood cells, lymphocytes, and monocytes; each variable increased (P <= 0.01) 2 h postchallenge. Additionally, neutrophil counts decreased (P <= 0.01) in response to CRH/VP challenge in both treatment groups. Glucose concentrations within the LM were greater (P = 0.03) in CON when compared with ZIL. Lactate concentrations and GP within the BF were greater in CON (P = 0.05) when compared with ZIL. These data suggest there are some variations observed between treatments in terms of response to the CRH/VP challenge; however, in the environmental conditions of this trial, none of the variations observed suggest that the supplementation of ZH detrimentally alters the ability of cattle to effectively respond to stressful stimuli. C1 [Buntyn, J. O.; Schmidt, T. B.; Erickson, G. E.; Sieren, S. E.; Jones, S. J.] Univ Nebraska, Dept Anim Sci, Lincoln, NE 68583 USA. [Sanchez, N. C. Burdick; Carroll, J. A.] ARS, Livestock Issues Res Unit, USDA, Lubbock, TX 79403 USA. RP Carroll, JA (reprint author), ARS, Livestock Issues Res Unit, USDA, Lubbock, TX 79403 USA. EM jeff.carroll@ars.usda.gov FU Merck Animal Health, Summit, NJ FX The authors acknowledge the technical support of J. W. Dailey, J. R. Carroll, and C. Wu (USDA-ARS) and T. F. Jones, D. B. Burken, and C. J. Bittner (University of Nebraska-Lincoln). Research was partially funded by Merck Animal Health, Summit, NJ. NR 41 TC 0 Z9 0 U1 4 U2 4 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUL PY 2016 VL 94 IS 7 BP 2798 EP 2810 DI 10.2527/jas2015-0192 PG 13 WC Agriculture, Dairy & Animal Science SC Agriculture GA DY3PE UT WOS:000385005300011 PM 27482667 ER PT J AU Kerr, BJ Dozier, WA Shurson, GC AF Kerr, B. J. Dozier, W. A., III Shurson, G. C. TI Lipid digestibility and energy content of distillers' corn oil in swine and poultry SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE broilers; digestibility; distillers' corn oil; energy; pigs ID APPARENT METABOLIZABLE ENERGY; GROWING-FINISHING PIGS; DIFFERENT FAT SOURCES; CHEMICAL-COMPOSITION; BROILER-CHICKENS; DIETARY-FAT; ACID OILS; POSTWEANING SWINE; NURSERY PIGS; DRIED GRAINS AB Two experiments were conducted to determine the DE and ME and apparent total tract digestibility of ether extract of 3 distillers' corn oil (DCO; 4.9, 12.8, or 13.9% free fatty acids [FFA]) samplescompared with a sample of refined corn oil (CO; 0.04% FFA) and an industrially hydrolyzed high-FFA DCO (93.8% FFA) in young pigs and growing broilers. In Exp. 1, 54 barrows (initial age = 28 d) were fed a common diet for 7 d and then fed their allotted dietary treatment (either 100% basal diet or 1 of 5 test diets consisting of 90% basal diet plus 10% test lipid) for the next 7 d in group pens (9 pigs/pen). For the next 10 d, pigs were moved to individual metabolism crates for continued diet and crate adaptation and to a twice-daily feeding regimen. Pigs remained on their respective diets for a 4-d total fecal and urine collection period. For Exp. 2, 567 male broilers were obtained from a commercial hatchery (1 d of age) and reared in grower battery cages that contained 9 chicks per cage. Broilers were fed a common corn-soybean meal starter diet from placement until the beginning of the trial (19 d of age). Birds were then randomly assigned to 1 of 6 dietary treatments (94% basal diet plus 6% dextrose or 94% basal diet plus 6% test lipid substituted for dextrose) on d 19 and were allowed an 8-d dietary acclimation period followed by a 48-h energy balance assay. In Exp. 1, the DCO sample with 12.8% FFA contained the lowest (P < 0.05) DE (8,036 kcal/kg) content compared with the 0.04% refined CO sample and the 4.9 or 93.8% FFA DCO samples (8,814, 8,828, and 8,921 kcal/kg, respectively), with the DCO source containing 13.9% FFA having intermediate DE (8,465 kcal/kg) content. The ME content of these lipid sources also differed among treatments (P < 0.01), following trends similar to their DE values, with no differences noted for ME as a percentage of DE (P > 0.35) content among the lipids evaluated. In Exp. 2, lipids containing 0.04, 4.9, 12.8, and 13.9% FFA had similar nitrogen corrected apparent ME (AME(n)) values (8,072, 7,936, 8,036, and 7,694 respectively), except for the industrially hydrolyzed DCO sample containing 93.8% FFA, which contained 6,276 kcal/kg (P < 0.01). Using published prediction equations, the predicted DE of these lipids for swine was 3.5% greater than the values determined in Exp. 1 for all lipid sources, except for the DCO sample containing 93.8% FFA, which the predicted DE was underestimated. Likewise, the predicted AME(n) of these lipids for broilers was 7.4% greater than the determined AME(n) (Exp. 2) for all lipid sources. C1 [Kerr, B. J.] ARS, USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Dozier, W. A., III] Auburn Univ, Dept Poultry Sci, Auburn, AL 36849 USA. [Shurson, G. C.] Univ Minnesota, Dept Anim Sci, St Paul, MN 55108 USA. RP Kerr, BJ (reprint author), ARS, USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA. EM brian.kerr@ars.usda.gov FU POET Nutrition LLC FX This research was financially supported in part by POET Nutrition LLC. Mention of a trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty by the USDA, Auburn University, or the University of Minnesota and does not imply approval to the exclusion of other products that may be suitable. The USDA is an equal opportunity provider and employer. NR 58 TC 1 Z9 1 U1 5 U2 5 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUL PY 2016 VL 94 IS 7 BP 2900 EP 2908 DI 10.2527/jas2016-0440 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA DY3PE UT WOS:000385005300020 PM 27482676 ER PT J AU Hales, KE Foote, AP Jones, SA Shackelford, SD Boyd, BM Erickson, GE AF Hales, K. E. Foote, A. P. Jones, S. A. Shackelford, S. D. Boyd, B. M. Erickson, G. E. TI The effects of zilpaterol hydrochloride and shade on blood metabolites of finishing beef steers SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE blood metabolites; feedlot cattle; stress; zilpaterol hydrochloride ID BETA-AGONIST; PERFORMANCE; TEMPERATURE; STRESS; PLASMA; MUSCLE AB The effects of feeding zilpaterol hydrochloride (ZH) and shade were evaluated on blood metabolites in finishing beef steers (n = 480). Cattle were fed 0 or 8.33 mg/kg of diet DM ZH for 21 d with a 3-or 4-d withdrawal before harvest and were housed in open or shaded pens. Blood samples were collected the day before ZH was fed and on the day the cattle were shipped to the commercial abattoir. Lactate concentration was not different between cattle fed ZH in open or shaded pens (P = 0.12). Nonetheless, a tendency for a diet x time interaction was detected for lactate concentration (P = 0.09), in which it was greater in cattle fed the control diet in open pens before being fed ZH. Cortisol concentration was less before and after ZH was fed (P = 0.01). Glucose was greater for cattle fed the control diet than cattle fed ZH for 21 d (P = 0.03). Cattle fed in open vs. shaded pens did not differ in glucose concentration (P = 0.12), whereas glucose concentrations were greater before ZH was fed than after (P = 0.02). In contrast, plasma urea nitrogen (PUN) concentration was not different in response to diet (P = 0.24), housing type (P = 0.65), or before vs. after being fed ZH (P = 0.60). Lactate concentrations were not different across diet or shade treatments before ZH was fed, whereas after ZH, lactate concentrations were greater in control cattle than cattle fed ZH. Additionally, cortisol was less after feeding ZH. Glucose was greater before than after feeding ZH. C1 [Hales, K. E.; Foote, A. P.; Jones, S. A.; Shackelford, S. D.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Boyd, B. M.; Erickson, G. E.] Univ Nebraska, Dept Anim Sci, Lincoln, NE 68583 USA. RP Hales, KE (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM Kristin.Hales@ars.usda.gov NR 14 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUL PY 2016 VL 94 IS 7 BP 2937 EP 2941 DI 10.2527/jas2015-9967 PG 5 WC Agriculture, Dairy & Animal Science SC Agriculture GA DY3PE UT WOS:000385005300024 PM 27482680 ER PT J AU Foote, AP Freetly, HC AF Foote, A. P. Freetly, H. C. TI Effect of abomasal butyrate infusion on net nutrient flux across the portal-drained viscera and liver of growing lambs SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE blood flow; butyrate; nutrient flux; portal-drained viscera; sheep ID VOLATILE FATTY-ACIDS; INCREASING RUMINAL BUTYRATE; WASHED RETICULORUMEN; RUMEN DEVELOPMENT; BLOOD-FLOW; SPLANCHNIC METABOLISM; SODIUM-BUTYRATE; NEWBORN CALVES; STEERS; CELLS AB The purpose of this experiment was to determine if supplying butyrate to the postruminal gastrointestinal tract of growing lambs alters blood flow and nutrient flux across the portal-drained viscera (PDV) and hepatic tissues. Polled Dorset wether lambs (n = 10; initial BW = 55 +/- 3.3 kg) had catheters surgically implanted into the portal vein, a branch of the hepatic vein, a mesenteric vein, and the abdominal aorta. A cannula was placed in the abomasum to deliver the treatment. Lambs were fed a pelleted ration once daily consisting of 69.7% dehydrated alfalfa, 30.0% ground corn, and 0.3% salt at 1.3 x NEm requirement. The experimental design was a crossover balanced in time, so that each lamb received both treatments. Treatments consisted of either a pulse dose infusion of butyrate (buffered solution) to supply butyrate (10 mg/kg BW) or a buffered saline solution (1 mL/kg BW) once daily at the time of feeding. On d 14 of the treatment period, nutrient fluxes were measured using para-aminohippuric acid as a blood flow marker. Blood samples were collected from the aorta, portal vein, and hepatic vein every hour for 9 h beginning at 30 min prior to treatment/feeding. There was a tendency for a treatment x time interaction (P = 0.05) for portal vein blood flow, indicating that blood flow began to decrease earlier postprandial in lambs receiving butyrate. The butyrate treatment tended to increase the uptake of O-2 (P = 0.07) and increased the uptake of glucose (P = 0.002), glutamate (P = 0.04), and glutamine (P = 0.02) by the PDV. There was a treatment x time interaction (P < 0.01) for flux of acetate, propionate, butyrate, isobutyrate, and valerate across the PDV. The interaction was mainly due to an earlier postprandial peak and associated decrease in the flux rate of the VFA. The alteration in timing of the postprandial peak of VFA flux was also observed in hepatic fluxes of VFA. It appears that supplying butyrate to the postruminal tissues through an abomasal cannula increases glucose, glutamate, and glutamine metabolism by the PDV. C1 [Foote, A. P.; Freetly, H. C.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Foote, AP (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM Andrew.Foote@ars.usda.gov NR 31 TC 0 Z9 0 U1 3 U2 3 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUL PY 2016 VL 94 IS 7 BP 2962 EP 2972 DI 10.2527/jas2016-0485 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA DY3PE UT WOS:000385005300027 PM 27482683 ER PT J AU Stommel, JR Camp, MJ Dumm, JM Haynes, KG Luo, YG Schoevaars, AM AF Stommel, John R. Camp, Mary J. Dumm, Judith M. Haynes, Kathleen G. Luo, Yaguang Schoevaars, Anne Marie TI Heritability of Fresh-cut Fruit Quality Attributes in Capsicum SO JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE LA English DT Article DE electrolyte leakage; heritability; germplasm; modified atmosphere packaging; pepper; shelf life ID SHELF-LIFE; POSTHARVEST BIOLOGY; BELL PEPPERS; LETTUCE; STORAGE; LEAVES; OXYGEN AB Fresh pepper (Capsicum) fruit that are sliced and/or diced are referred to as fresh-cut products. The current report evaluates the inheritance of postharvest attributes that contribute to pepper fresh-cut quality. Marketable green fruit of large-fruited Capsicum annuum accessions with bell and related pod types (Class 1), C. annuum accessions with jalapeno and serrano pod types (Class 2), and thin-walled "aji''-like tabasco pod types from Capsicum baccatum, Capsicum frutescens, and Capsicum chinense (Class 3) were processed and stored up to 14 days in selective oxygen transmission rate packaging. Fresh-cut attributes were influenced by genotype as well as year. For all pod types, O-2 and CO2 partial pressures in storage packages, tissue weight loss, and electrolyte leakage differed among accessions, days of storage, and years of testing. Percent O-2 declined and CO2 and electrolyte leakage generally increased during storage. Some accessions in Class 1 and Class 2 maintained acceptable product quality during storage. Changes in fruit weight loss were small with greater weight loss observed in Class 1 accessions relative to weight loss for Class 2 and Class 3. Broad-sense heritability for fresh-cut attributes was moderate to low indicating that it will be difficult to breed for fresh-cut quality. C1 [Stommel, John R.; Dumm, Judith M.; Haynes, Kathleen G.] ARS, Genet Improvement Fruits & Vegetables Lab, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Camp, Mary J.] ARS, Biometr Consulting Serv, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Luo, Yaguang] ARS, Food Qual Lab, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Schoevaars, Anne Marie] Enza Zaden Res USA Inc, 525 Lucy Brown Lane, San Juan Bautista, CA 95045 USA. RP Stommel, JR (reprint author), ARS, Genet Improvement Fruits & Vegetables Lab, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM john.stommel@ars.usda.gov NR 28 TC 0 Z9 0 U1 3 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0003-1062 EI 2327-9788 J9 J AM SOC HORTIC SCI JI J. Am. Soc. Hortic. Sci. PD JUL PY 2016 VL 141 IS 4 BP 308 EP 314 PG 7 WC Horticulture SC Agriculture GA DY3PB UT WOS:000385005000001 ER PT J AU Bai, JH Baldwin, E Hearn, J Driggers, R Stover, E AF Bai, Jinhe Baldwin, Elizabeth Hearn, Jack Driggers, Randy Stover, Ed TI Volatile and Nonvolatile Flavor Chemical Evaluation of USDA Orange-Mandarin Hybrids for Comparison to Sweet Orange and Mandarin Fruit SO JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE LA English DT Article DE citrus; aroma; sesquiterpene; interaction of flavor compounds; sugar; acid ID CANDIDATUS LIBERIBACTER ASIATICUS; GAS-CHROMATOGRAPHY-OLFACTOMETRY; KEY AROMA COMPONENTS; REAL-TIME PCR; JUICE MATRIX; CITRUS; ODOR; PERCEPTION; QUALITY; FRESH AB Three citrus hybrids, containing 50% to 75% sweet orange (Citrus sinensis) genome in their pedigrees and similar to sweet orange in fruit size, color, and taste, were tested for their potential to be classified as new "sweet orange'' cultivars. 'Hamlin', 'Midsweet', and three other early to midseason sweet oranges, along with 'Dancy' tangerine (Citrus reticulata), a typical mandarin, were used for comparison. Fruit were picked on 23 Jan. 2014, 30 Dec. 2014, and 27 Jan. 2015. A total of 114 volatiles were detected and separated into seven groups by detection frequency: three groups with 43 volatile components did not show differences and thus contributed little information for classification of sweet orange vs. mandarin, and the remaining four groups with 71 volatiles contributed to distinctions between orange and mandarin. Among the hybrids, the pattern of volatile detection frequency for hybrid FF-1-74-52 was virtually identical to sweet orange, and cluster analysis agreed with the classification. The number of average peaks were 55 to 62 in sweet oranges, 67 in FF-1-74-52, and 17 to 37 in tangerine and other hybrids. Quantity analysis of individual volatiles and chemical classes indicated that FF-1-74-52 and sweet oranges were rich in total volatile abundance, and almost all chemical classes including mono and sesquiterpenes, aldehydes, alcohols, ketones, and esters. This was especially true for ethyl butanoate, which contributes a fruity top note, and valencene and all sesquiterpene hydrocarbons, which only contribute to citrus flavor indirectly through their contribution to headspace partitioning. Two other hybrids, FF-1-75-55 and FF-1-76-51, each had some similarity to sweet oranges in several chemicals and classes, but not in the overall volatile profile. All three sweet orange-like hybrids met the standards for mandarins and oranges in soluble solids content, titratable acidity (TA), and the ratio. The above volatile and nonvolatile flavor chemical profile comparisons strongly support a proposal to classify FF-1-74-52 as a "sweet orange'' commercially, and all three hybrids were previously shown to be more similar to sweet orange in their volatile profile than is 'Ambersweet'. 'Ambersweet' was a hybrid that was legally classified as a "sweet orange'' in 1995 based on its volatile profile. C1 [Bai, Jinhe; Baldwin, Elizabeth; Hearn, Jack; Driggers, Randy; Stover, Ed] ARS, USDA, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. RP Bai, JH (reprint author), ARS, USDA, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM jinhe.bai@ars.usda.gov NR 40 TC 0 Z9 0 U1 3 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0003-1062 EI 2327-9788 J9 J AM SOC HORTIC SCI JI J. Am. Soc. Hortic. Sci. PD JUL PY 2016 VL 141 IS 4 BP 339 EP 350 PG 12 WC Horticulture SC Agriculture GA DY3PB UT WOS:000385005000004 ER PT J AU Cerny, KC Stahle, DW Bragg, DC AF Cerny, K. Chris Stahle, David W. Bragg, Don C. TI A frontier shortleaf pine stand in the old-growth Cross Timbers of Oklahoma SO JOURNAL OF THE TORREY BOTANICAL SOCIETY LA English DT Article DE Cross Timbers; old-growth; range expansion; shortleaf pine ID HISTORIC FIRE REGIME; UNITED-STATES; QUERCUS-MARILANDICA; BOSTON MOUNTAINS; PREDICTIVE MODEL; ANCIENT FORESTS; UPLAND FORESTS; DYNAMICS; ARKANSAS; OAK AB We investigated an old-growth oak-shortleaf pine (Pinus echinata) stand of high ecological integrity in east-central Oklahoma located west of the continuous native distribution of shortleaf pine. With the exception of an abundance of shortleaf pine, the basal area (17.2 m(2)/ha), density (559.6 trees/ha), and species composition of this unmanaged stand is similar to other old hardwood forests in the Cross Timbers ecoregion. Tree-ring dating indicates that the oldest post oak (Quercus stellata) and shortleaf pine sampled in Shortleaf Canyon were 262 and 230 yr old, respectively. Dendrochronology indicated that drought-related disturbances contributed to several episodic pulses of natural regeneration of shortleaf pine at this site. Coupled with local historical documentation and regional paleoecological evidence, the absence of older pines at this isolated site suggests that P. echinata may have naturally colonized Shortleaf Canyon recently, perhaps during the last 250 yr. C1 [Cerny, K. Chris] Texas Parks & Wildlife Dept, Austin, TX 78744 USA. [Stahle, David W.] Univ Arkansas, Dept Geosci, Fayetteville, AR 72701 USA. [Bragg, Don C.] US Forest Serv, USDA, Southern Res Stn, Monticello, AR 71656 USA. RP Cerny, KC (reprint author), Texas Parks & Wildlife Dept, Austin, TX 78744 USA. EM chris.cerny@tpwd.texas.gov FU Ozark Society; Southern Research Station of the USDA Forest Service FX The Ozark Society is gratefully acknowledged for awarding the Neil Compton Scholarship to Chris Cerny, which helped fund this research. Additional funding support for this research also came from the Southern Research Station of the USDA Forest Service. NR 71 TC 0 Z9 0 U1 1 U2 1 PU TORREY BOTANICAL SOC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 1095-5674 EI 1940-0616 J9 J TORREY BOT SOC JI J. Torrey Bot. Soc. PD JUL PY 2016 VL 143 IS 3 BP 224 EP 238 DI 10.3159/TORREY-D-15-00043.1 PG 15 WC Plant Sciences SC Plant Sciences GA DY2OQ UT WOS:000384932300002 ER PT J AU Gustafson, DJ Cruse-Sanders, JM Bucalo, K Alley, H Kunz, M Alexander, ML Vankus, V Duplantier, M AF Gustafson, Danny J. Cruse-Sanders, Jennifer M. Bucalo, Kylie Alley, Heather Kunz, Michael Alexander, Mara L. Vankus, Victor Duplantier, Martin TI Survey of genetic diversity and seed germination rates of the southeastern endemic Symphyotrichum georgianum (Alexander) GL Nesom (Asteraceae) from large and small populations SO JOURNAL OF THE TORREY BOTANICAL SOCIETY LA English DT Article DE candidate conservation agreement; genetic structure; Georgia aster; seed germination; single nucleotide polymorphism (SNP) ID SINGLE NUCLEOTIDE POLYMORPHISMS; NEXT-GENERATION; WILD RELATIVES; CLONAL GROWTH; POOLED DNA; PLANT; SUNFLOWER; CONSERVATION; MARKERS; DIFFERENTIATION AB Single nucleotide polymorphic (SNP) markers were used to characterize the genetic structure of Symphyotrichum georgianum (Alexander) G.L. Nesom, known more commonly as the Georgia aster, from 26 populations across the species' range. This species is considered vulnerable (G3) and was a candidate for listing under the Endangered Species Act. We sampled large (> 500 stems) and small (< 100 stems) populations from across the entire range for the species. Next generation sequencing was used to assess 36 SNP markers for pooled population samples, consisting of equal amounts of genomic DNA from 30 individual stems sampled across each population. Most of the genetic variation was partitioned within and less among populations, which is consistent with perennial outcrossing species. There was a significant (r = 0.506, P < 0.001) positive association between geographic distance and genetic distance among populations, indicating increasing genetic distance (genetic dissimilarity) with increasing geographic distances between populations. There was no statistically significant difference in genetic diversity between large and small populations, but trends were observed. The Georgia and North Carolina large populations were more genetically diverse than the small populations, while the small populations were more genetically diverse than the large populations in Alabama and South Carolina. Symphyotrichum georgianum is an outcrossing perennial aster with a rhizomatous growth habit. Extensive clonal growth could account for the low genetic diversity estimates from large populations; however, quantifying the extent of clonal growth within sites is beyond the scope of the current study. There was no association between genetic diversity measures and seed fill or germination rates. Additional fine scale genetic structure studies are underway to address the extent of clonal growth in these remnant populations and how that may affect viable seed production. C1 [Gustafson, Danny J.] Citadel, Dept Biol, Charleston, SC 29409 USA. [Cruse-Sanders, Jennifer M.; Bucalo, Kylie] Atlanta Bot Garden, 1345 Piedmont Ave NE, Atlanta, GA 30309 USA. [Alley, Heather] State Bot Garden Georgia, Mimsie Lanier Ctr Native Plant Studies, 2450 South Milledge Ave, Athens, GA 30605 USA. [Kunz, Michael] Univ North Carolina Chapel Hill, North Carolina Bot Garden, CB3375, Chapel Hill, NC 27599 USA. [Alexander, Mara L.] US Fish & Wildlife Serv, Asheville Ecol Serv Field Off, 160 Zillicoa St, Asheville, NC 28801 USA. [Vankus, Victor] US Forest Serv, USDA, Natl Seed Lab, 5675 Riggins Mill Rd, Dry Branch, GA 31020 USA. [Duplantier, Martin] Citadel, Dept Biol, Charleston, SC 29409 USA. RP Gustafson, DJ (reprint author), Citadel, Dept Biol, Charleston, SC 29409 USA. EM danny.gustafson@citadel.edu FU U.S. Department of Agriculture Forest Service [10-CS-11080300-019]; U.S. Fish and Wildlife Service [40181AJ197]; Citadel Foundation FX This research was funded by grants from U.S. Department of Agriculture Forest Service 10-CS-11080300-019 to J.C.S., U.S. Fish and Wildlife Service 40181AJ197 to D.J.G., and The Citadel Foundation to D.J.G. NR 61 TC 0 Z9 0 U1 4 U2 4 PU TORREY BOTANICAL SOC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 1095-5674 EI 1940-0616 J9 J TORREY BOT SOC JI J. Torrey Bot. Soc. PD JUL PY 2016 VL 143 IS 3 BP 274 EP 284 DI 10.3159/TORREY-D-15-00026.1 PG 11 WC Plant Sciences SC Plant Sciences GA DY2OQ UT WOS:000384932300006 ER PT J AU Khalil, RKS Skinner, C Patfield, S He, XH AF Khalil, Rowaida K. S. Skinner, Craig Patfield, Stephanie He, Xiaohua TI Phage-mediated Shiga toxin (Stx) horizontal gene transfer and expression in non-Shiga toxigenic Enterobacter and Escherichia coli strains SO PATHOGENS AND DISEASE LA English DT Article DE ELISA; Enterobacter cloacae; Escherichia coli O157; horizontal gene transfer; Stx-converting bacteriophages; transduction ID HEMOLYTIC-UREMIC SYNDROME; ENCODING BACTERIOPHAGES; 2-ENCODING BACTERIOPHAGE; TEMPERATE BACTERIOPHAGE; FOODBORNE PATHOGENS; INSERTION SITES; SHIGELLA-SONNEI; IN-VITRO; DIVERSITY; EVOLUTION AB Enterobacter cloacae M12X01451 strain recently identified from a clinical specimen produces a new Stx1 subtype (Stx1e) that was not neutralized by existing anti-Stx1 monoclonal antibodies. Acquisition of stx by Ent. cloacae is rare and origin/stability of stx(1e) in M12X01451 is not known. In this study, we confirmed the ability of Stx1a- and Stx1e-converting phages from an Escherichia coli O157:H7 strain RM8530 and M12X01451 respectively to infect several E. coli and Ent. cloacae strains. stx(1e) was detected in 97.5% and 72.5% of progenies of strains lysogenized by stx(1e) phage after 10 (T-10) and 20 (T-20) subcultures, versus 65% and 17.5% for stx(1a) gene. Infection of M12X01451 and RM8530 with each other's phages generated double lysogens containing both phages. stx(1a) was lost after T-10, whereas the stx(1e) was maintained even after T-20 in M12X01451 lysogens. In RM8530 lysogens, the acquired stx(1e) was retained with no mutations, but 20% of stx(1a) was lost after T-20. ELISA and western blot analyses demonstrated that Stx1e was produced in all strains lysogenized by stx(1e) phage; however, Stx1a was not detected in any lysogenized strain. The study results highlight the potential risks of emerging Stx-producing strains via bacteriophages either in the human gastrointestinal tract or in food production environments, which are matters of great concern and may have serious impacts on human health. C1 [Khalil, Rowaida K. S.] Univ Alexandria, Fac Sci, Dept Bot & Microbiol, Alexandria 21511, Egypt. [Skinner, Craig; Patfield, Stephanie; He, Xiaohua] ARS, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. RP He, XH (reprint author), ARS, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM xiaohua.he@ars.usda.gov FU U.S. Department of Agriculture, Agricultural Research Service CRIS project [2030-42000-048-00D] FX This study was supported by U.S. Department of Agriculture, Agricultural Research Service CRIS project 2030-42000-048-00D. NR 84 TC 1 Z9 1 U1 5 U2 5 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 2049-632X J9 PATHOG DIS JI Pathog. Dis. PD JUL PY 2016 VL 74 IS 5 AR ftw037 DI 10.1093/femspd/ftw037 PG 11 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA DY3TX UT WOS:000385018700005 ER PT J AU Jansky, SH Charkowski, AO Douches, DS Gusmini, G Richael, C Bethke, PC Spooner, DM Novy, RG De Jong, H De Jong, WS Bamberg, JB Thompson, AL Bizimungu, B Holm, DG Brown, CR Haynes, KG Sathuvalli, VR Veilleux, RE Miller, JC Bradeen, JM Jiang, JM AF Jansky, Shelley H. Charkowski, Amy O. Douches, David S. Gusmini, Gabe Richael, Craig Bethke, Paul C. Spooner, David M. Novy, Richard G. De Jong, Hielke De Jong, Walter S. Bamberg, John B. Thompson, A. L. Bizimungu, Benoit Holm, David G. Brown, Chuck R. Haynes, Kathleen G. Sathuvalli, Vidyasagar R. Veilleux, Richard E. Miller, J. Creighton, Jr. Bradeen, Jim M. Jiang, Jiming TI Reinventing Potato as a Diploid Inbred Line-Based Crop SO CROP SCIENCE LA English DT Article ID WILD-SPECIES HYBRIDS; BACKCROSS QTL ANALYSIS; SOLANUM-TUBEROSUM; QUANTITATIVE TRAIT; PHYTOPHTHORA-INFESTANS; CULTIVATED POTATOES; SEED FAMILIES; SLI GENE; MAXIMUM HETEROZYGOSITY; TETRAPLOID POTATOES AB The third most important food crop worldwide, potato (Solanum tuberosum L.) is a tetraploid outcrossing species propagated from tubers. Breeders have long been challenged by polyploidy, heterozygosity, and asexual reproduction. It has been assumed that tetraploidy is essential for high yield, that the creation of inbred potato is not feasible, and that propagation by seed tubers is ideal. In this paper, we question those assumptions and propose to convert potato into a diploid inbred line-based crop propagated by true seed. Although a conversion of this magnitude is unprecedented, the possible genetic gains from a breeding system based on inbred lines and the seed production benefits from a sexual propagation system are too large to ignore. We call on leaders of public and private organizations to come together to explore the feasibility of this radical and exciting new strategy in potato breeding. C1 [Jansky, Shelley H.; Bethke, Paul C.; Spooner, David M.] Univ Wisconsin, Dept Hort, USDA ARS, Vegetable Crops Res Unit, 1575 Linden Dr, Madison, WI 53706 USA. [Charkowski, Amy O.] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA. [Douches, David S.] Mich State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI USA. [Gusmini, Gabe] Pepsico, St Paul, MN USA. [Richael, Craig] Simplot Plant Sci, Boise, ID USA. [Novy, Richard G.] USDA ARS, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID USA. [De Jong, Hielke; Bizimungu, Benoit] Agr & Agri Food Canada, Fredericton, NB, Canada. [De Jong, Walter S.] Cornell Univ, Sch Integrat Plant Sci, Ithaca, NY USA. [Bamberg, John B.] Univ Wisconsin, Dept Hort, USDA ARS, Madison, WI 53706 USA. [Bamberg, John B.] US Potato Genebank, Sturgeon Bay, WI USA. [Thompson, A. L.] North Dakota State Univ, Dept Plant Sci, Fargo, ND USA. [Holm, David G.] Colorado State Univ, Dept Hort & Landscape Architecture, San Luis Valley Res Ctr, Ft Collins, CO 80523 USA. [Brown, Chuck R.] USDA ARS, Prosser, WA 99350 USA. [Haynes, Kathleen G.] USDA ARS, Beltsville, MD USA. [Sathuvalli, Vidyasagar R.] Oregon State Univ, Dept Crop & Soil Sci, Hermiston Agr Res & Extens Ctr, Hermiston, OR USA. [Veilleux, Richard E.] Virginia Tech, Dept Hort, Blacksburg, VA USA. [Miller, J. Creighton, Jr.] Texas A&M Univ, Dept Hort Sci, College Stn, TX 77843 USA. [Bradeen, Jim M.] Univ Minnesota, Dept Plant Pathol, St Paul, MN USA. [Jiang, Jiming] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. RP Jansky, SH (reprint author), Univ Wisconsin, Dept Hort, USDA ARS, Vegetable Crops Res Unit, 1575 Linden Dr, Madison, WI 53706 USA. EM shelley.jansky@ars.usda.gov NR 121 TC 0 Z9 0 U1 6 U2 6 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 1412 EP 1422 DI 10.2135/cropsci2015.12.0740 PG 11 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700002 ER PT J AU Sukumaran, S Li, X Li, XR Zhu, CS Bai, GH Perumal, R Tuinstra, MR Prasad, PVV Mitchell, SE Tesso, TT Yu, JM AF Sukumaran, Sivakumar Li, Xin Li, Xianran Zhu, Chengsong Bai, Guihua Perumal, Ramasamy Tuinstra, Mitchell R. Prasad, P. V. Vara Mitchell, Sharon E. Tesso, Tesfaye T. Yu, Jianming TI QTL Mapping for Grain Yield, Flowering Time, and Stay-Green Traits in Sorghum with Genotyping-by-Sequencing Markers SO CROP SCIENCE LA English DT Article ID BICOLOR L. MOENCH; GENOME-WIDE ASSOCIATION; MAIZE ZEA-MAYS; DROUGHT TOLERANCE; PLANT HEIGHT; SPRING WHEAT; TRANSPIRATION EFFICIENCY; CANOPY DEVELOPMENT; CROP IMPROVEMENT; TROPICAL SORGHUM AB Molecular breeding can complement traditional breeding approaches to achieve genetic gains in a more efficient way. In the present study, genetic mapping was conducted in a sorghum recombinant inbred line (RIL) population developed from Tx436 (a non-stay-green high food quality inbred) x 00MN7645 (a stay-green high yield inbred) and evaluated in eight environments (location and year combination) in a hybrid background of Tx3042 (a non-stay-green A-line). Phenotyping was conducted for agronomic traits (grain yield and flowering time), physiological traits of stay-green (chlorophyll content [SPAD] and chlorophyll fluorescence [F-v/F-m] measured on the leaves), and green leaf area visual score (GLAVS). This population was genotyped with genotyping-by-sequencing (GBS) technology. Data processing resulted in 7144 high quality single nucleotide polymorphisms (SNPs) that were used in a genome-wide single marker scan with physical distance. A selected subset of 1414 SNPs was used for composite interval mapping (CIM) with genetic distance. These complementary methods revealed fifteen QTLs for the traits studied. In addition, QTL mapping for individual environments and year-wise combinations revealed 42 QTLs. A consistent QTL for grain yield under normal and stressed conditions was identified in chromosome 1 that explained 8 to 16% of the phenotypic variation. QTLs for flowering time were identified in chromosomes 2, 6, and 9 that explained 6 to 11% of the phenotypic variation. Stay-green QTLs in chromosomes 3 and 4 explained 8 to 24% of the phenotypic variation. These identified QTLs with flanking SNPs of known genomic positions could be used to improve grain yield, flowering time, and stay-green in sorghum molecular breeding programs. C1 [Sukumaran, Sivakumar; Li, Xin; Li, Xianran; Zhu, Chengsong; Bai, Guihua; Perumal, Ramasamy; Prasad, P. V. Vara; Tesso, Tesfaye T.; Yu, Jianming] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Bai, Guihua] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Tuinstra, Mitchell R.] Dept Agron, 915 West State St, W Lafayette, IN 47907 USA. [Mitchell, Sharon E.] Cornell Univ, Inst Genom Divers, Ithaca, NY 14853 USA. RP Yu, JM (reprint author), Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. EM jmyu@iastate.edu FU Agriculture and Food Research Initiative Competitive Grant from USDA National Institute of Food and Agriculture [2011-03587]; Kansas Grain Sorghum Commission; Kansas State University Center for Sorghum Improvement FX This work was supported by the Agriculture and Food Research Initiative Competitive Grant (2011-03587) from USDA National Institute of Food and Agriculture, Kansas Grain Sorghum Commission, and Kansas State University Center for Sorghum Improvement. NR 116 TC 0 Z9 0 U1 15 U2 15 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 1429 EP 1442 DI 10.2135/cropsci2015.02.0097 PG 14 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700004 ER PT J AU Hall, BD Fox, R Zhang, Q Baumgarten, A Nelson, B Cummings, J Drake, B Phillips, D Hayes, K Beatty, M Zastrow-Hayes, G Zeka, B Hazebroek, J Smith, S AF Hall, Bradford D. Fox, Richard Zhang, Qu Baumgarten, Andy Nelson, Barry Cummings, Joe Drake, Ben Phillips, Debora Hayes, Kevin Beatty, Mary Zastrow-Hayes, Gina Zeka, Brian Hazebroek, Jan Smith, Stephen TI Comparison of Genotypic and Expression Data to Determine Distinctness among Inbred Lines of Maize for Granting of Plant Variety Protection SO CROP SCIENCE LA English DT Article ID GENETIC-RELATIONSHIPS; DENT CORN; HYBRIDS; GERMPLASM; PATTERNS; GENOME; METABOLOMICS; GERMINATION; ENVIRONMENT; DIVERSITY AB The Union Internationale pour la Protection des Obtentions Vegetales (UPOV) currently relies on morphological characteristics to evaluate distinctness, uniformity, and stability (DUS) as eligibility requirements for the granting of Plant Variety Protection (PVP). We used 10 maize (Zea mays L.) inbred lines, including both unrelated and closely similar pairs, representing three heterotic groups to compare abilities of morphological, ribonucleic acid (RNA) transcription, metabolomic, and single nucleotide polymorphism (SNP) data to distinguish inbred lines. We used the range of variability and robustness as important factors to determine distinguishing power of each methodological approach. Using an index that ranged from 0 to 100 (useless to the perfect ideal), index scores for each methodology were: metabolomics (0), RNA transcription (18.2), morphology (19.6), and SNPs (35.7). The ability to distinguish among genotypes using RNA transcription expression data was concordant with SNP data for genotypes that were up to 97.2% similar according to SNPs. The SNP data alone could provide the basis for a determination of distinctness among inbred lines of maize with use of morphological, physiological, or agronomic performance data as supplementary information, if needed. C1 [Hall, Bradford D.] USDA ARS NCGRP, 1111 South Mason St, Ft Collins, CO 80521 USA. [Fox, Richard] 21 Homewood Dr, Kirkwood, MO 63122 USA. [Zhang, Qu; Baumgarten, Andy; Nelson, Barry; Cummings, Joe; Drake, Ben; Phillips, Debora; Hayes, Kevin; Beatty, Mary; Zastrow-Hayes, Gina; Zeka, Brian; Hazebroek, Jan] DuPont Pioneer, 8305 NW 62nd Ave, Johnston, IA 50131 USA. [Smith, Stephen] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Smith, Stephen] Iowa State Univ, Seed Sci Ctr, Ames, IA 50011 USA. RP Smith, S (reprint author), Iowa State Univ, Dept Agron, Ames, IA 50011 USA.; Smith, S (reprint author), Iowa State Univ, Seed Sci Ctr, Ames, IA 50011 USA. EM stephen.smith@mchsi.com NR 62 TC 0 Z9 0 U1 2 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 1443 EP 1459 DI 10.2135/cropsci2015.03.0185 PG 17 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700005 ER PT J AU Trapp, JJ Urrea, CA Zhou, JF Khot, LR Sankaran, S Miklas, PN AF Trapp, Jennifer J. Urrea, Carlos A. Zhou, Jianfeng Khot, Lav R. Sankaran, Sindhu Miklas, Phillip N. TI Selective Phenotyping Traits Related to Multiple Stress and Drought Response in Dry Bean SO CROP SCIENCE LA English DT Article ID MARKER-ASSISTED SELECTION; RESISTANCE; LOCI; TOLERANCE; LINKAGE; YIELD; POPULATION; GENOTYPES; GENOMICS; CROPS AB Abiotic stress tolerance in dry bean (Phaseolus vulgaris L.) is complex. Increased population sizes are contributing to finding QTL conditioning stress response but phenotyping has not kept pace with high throughput genotyping for such studies. Our objectives were to determine effectiveness of 20 most tolerant and 20 most susceptible lines representing phenotypic extremes from a RIL population ('Buster' x 'Roza' [BR]) to facilitate examination of 19 traits for relevance to stress response and to validate existing QTL conditioning stress response. Using phenotypic extremes tested across multiple trials, eight of the 19 traits were clearly associated with drought stress. Pod wall ratio (PW), plant biomass by weight or a visual rating, and greenness index (NDVI) were most associated with seed yield (SY) under stress followed by phenology traits. The phenotypic extreme lines were also useful for validating QTL previously identified in the whole RIL population conditioning SY, seed weight (SW) and days to flower (DF), harvest maturity (HM), and seed fill (DSF). New QTL were identified for biomass, PW, and NDVI which co-segregated with major QTL for seed yield SY1.1(BR) and SY2.1(BR). The preliminary finding of NDVI 1.1(BR) supports aerial imaging in larger genetic populations geared toward QTL analysis of stress response. In summary, phenotypic extremes helped sort through traits relevant to stress response in the Buster x Roza RIL population and verified the effect of two major QTL in response to terminal drought. C1 [Trapp, Jennifer J.] Washington State Univ, Crop & Soil Sci, Prosser, WA 99350 USA. [Urrea, Carlos A.] Univ Nebraska, Panhandle Res & Extens Ctr, 4502 Ave 1, Scottsbluff, NE 69361 USA. [Zhou, Jianfeng; Khot, Lav R.; Sankaran, Sindhu] Washington State Univ, Biol Syst Engn, POB 646120, Pullman, WA 99163 USA. [Miklas, Phillip N.] USDA ARS, Grain Legume Genet & Physiol Res Unit, 24106 N Bunn Rd, Prosser, WA 99350 USA. RP Miklas, PN (reprint author), USDA ARS, Grain Legume Genet & Physiol Res Unit, 24106 N Bunn Rd, Prosser, WA 99350 USA. EM phil.miklas@ars.usda.gov FU Norman Borlaug Commemorative Research Initiative [58-0210-3-012 (408-5354-012)]; Feed the Future Innovation Lab for Climate Resilient Beans Project [AID-OAA-A-13-00077] FX This work was supported, in part, by funding received from the Norman Borlaug Commemorative Research Initiative between US Agency for International Development and the USDA-ARS, through Cooperative Agreement No. 58-0210-3-012 (408-5354-012) and from the Feed the Future Innovation Lab for Climate Resilient Beans Project No. AID-OAA-A-13-00077. NR 44 TC 0 Z9 0 U1 9 U2 9 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 1460 EP 1472 DI 10.2135/cropsci2015.05.0281 PG 13 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700006 ER PT J AU Petersen, S Lyerly, JH Maloney, PV Brown-Guedira, G Cowger, C Costa, JM Dong, YH Murphy, JP AF Petersen, Stine Lyerly, Jeanette H. Maloney, Peter V. Brown-Guedira, Gina Cowger, Christina Costa, Jose M. Dong, Yanhong Murphy, J. Paul TI Mapping of Fusarium Head Blight Resistance Quantitative Trait Loci in Winter Wheat Cultivar NC-Neuse SO CROP SCIENCE LA English DT Article ID RECOMBINANT INBRED POPULATION; MARKER-ASSISTED SELECTION; TRITICUM-AESTIVUM L.; MOLECULAR CHARACTERIZATION; DEOXYNIVALENOL ACCUMULATION; SPRING WHEAT; QTL ANALYSIS; DNA MARKERS; MAJOR QTL; BARLEY AB Fusarium head blight (FHB), primarily caused by Fusarium graminearum, can significantly reduce the grain quality of wheat (Triticum aestivum L.) due to mycotoxin contamination. The objective of this study was to identify quantitative trait loci (QTL) for FHB resistance in the moderately resistant soft red winter wheat cultivar NC-Neuse. A total of 170 recombinant inbred lines (RILs) from a cross between NC-Neuse and the susceptible cultivar AGS 2000 were evaluated in inoculated, mist-irrigated field nurseries. The lines were evaluated for FHB incidence (INC), severity (SEV), Fusarium-damaged kernels (FDK), and deoxynivalenol (DON) content in seven environments between 2011 and 2014. A 3,419 cM linkage map was developed based on 1839 simple sequence repeat (SSR), diversity array technology (DArT), and single nucleotide polymorphism (SNP) markers. Seven FHB resistance QTL on chromosomes 1A, 1B, 1D, 2A, 4A, 5B, and 6A were mapped. The QTL alleles conferring resistance on 1A, 1B, 2A, 4A, and 6A originated from NC-Neuse, while the alleles associated with resistance on 1D and 5B originated from AGS 2000. Quantitative trait loci effects ranged from 9 to 12% for INC, from 6 to 11% for SEV, from 8 to 20% for FDK, and from 6 to 18% for DON. The QTL on 5B co-localized with the Vrn-B1 locus. Kompetitive Allele-Specific PCR (KASP) assays were developed for each NC-Neuse QTL region. A preliminary test using these assays on recent Uniform Southern Winter Wheat Nursery (USWWN) entries indicated Qfhb.nc-1A, Qfhb. nc-1B, and Qfhb. nc-6A as likely the best candidates for use in marker-assisted selection. C1 [Petersen, Stine; Lyerly, Jeanette H.; Murphy, J. Paul] North Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Brown-Guedira, Gina; Cowger, Christina] North Carolina State Univ, USDA ARS, Raleigh, NC USA. [Maloney, Peter V.] Monsanto Co, Galena, MD 21635 USA. [Costa, Jose M.] USDA ARS, Beltsville, MD 20705 USA. [Dong, Yanhong] Univ Minnesota, Dept Plant Pathol, Minneapolis, MN 55455 USA. RP Petersen, S (reprint author), North Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. EM stine.petersen13@gmail.com FU U.S. Department of Agriculture [58-6645-3-030]; North Carolina Small Grain Growers Association FX This material is based on work supported by the U.S. Department of Agriculture, under Agreement no. 58-6645-3-030. This is a cooperative project with the U.S. Wheat and Barley Scab Initiative. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U.S. Department of Agriculture. The work was also supported by the North Carolina Small Grain Growers Association. We thank Rene Navarro for the technical assistance in inoculum preparation as well as evaluation of the population in field nurseries. Dr. Consuelo Arellano from the Statistics Department and Dr. Thomas Isleib from the Department of Crop Science at NCSU were very kind in assisting with statistical analyses. We acknowledge Dr. Margaret Worthington for her help reviewing this paper. Finally, we thank Jared Smith, Kim Howell, and Sharon Williamson from the USDA-ARS Plant Science Research Unit in Raleigh, NC, for their technical assistance, and the USDA-ARS Cereal Crops Research Unit in Fargo, ND, where the SNP array genotyping was conducted. NR 65 TC 1 Z9 1 U1 6 U2 6 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 1473 EP 1483 DI 10.2135/cropsci2015.05.0312 PG 11 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700007 ER PT J AU Edwards, JW AF Edwards, Jode W. TI Genotype x Environment Interaction for Plant Density Response in Maize (Zea mays L.) SO CROP SCIENCE LA English DT Article ID RECIPROCAL RECURRENT SELECTION; NORTHERN CORN-BELT; DIFFERENT ERAS; HALF-SIB; POPULATION; YIELD; CULTIVARS; PERFORMANCE; IMPROVEMENT; ADAPTATION AB Increased adaptation to high plant density has been an important factor in improvements in grain yield in maize (Zea mays L.). Despite extensive public literature on variation and improvement in plant density response among maize varieties, much less public information is available on effects of environment and genotype x environment interactions (G x E) on plant density response for grain yield in maize. The present study was conducted to quantify environment effects and G x E effects on plant density response for maize grain yield. A set of 57 synthetic populations, synthetic x inbred line crosses, and synthetic x synthetic population crosses were evaluated across a five year period including 17 individual location-years. The data in this study were an unbalanced combination of multiple experiments that included 370 pedigree-environment combinations with each containing at least four planting densities and two replications per environment in most cases. G x E accounted for 26% of total genetic variance (genetic variance plus G x E variance) in linear regression coefficients and 39% of variance in quadratic regression coefficients in the regression of grain yield on observed plant density. Variance in G x E for linear and quadratic regression coefficients resulted in variation in optimal densities among varieties being highly specific to individual environments. Average plant density responses varied widely among environments with average optimal plant densities ranging from 5.6 to 9.1 plants m(-2). C1 [Edwards, Jode W.] Iowa State Univ, Dept Agron, USDA ARS CICGRU, Ames, IA 50011 USA. RP Edwards, JW (reprint author), Iowa State Univ, Dept Agron, USDA ARS CICGRU, Ames, IA 50011 USA. EM jode.edwards@ars.usda.gov NR 34 TC 1 Z9 1 U1 7 U2 7 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 1493 EP 1505 DI 10.2135/cropsci2015.07.0408 PG 13 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700009 ER PT J AU Zheng, XT Hoegenauer, KA Quintana, J Bell, AA Hulse-Kemp, AM Nichols, RL Stelly, DM AF Zheng, Xiuting Hoegenauer, Kevin A. Quintana, Jose Bell, Alois A. Hulse-Kemp, Amanda M. Nichols, Robert L. Stelly, David M. TI SNP-Based MAS in Cotton under Depressed-Recombination for Ren(lon)-Flanking Recombinants: Results and Inferences on Wide-Cross Breeding Strategies SO CROP SCIENCE LA English DT Article ID UPLAND COTTON; ROTYLENCHULUS-RENIFORMIS; GOSSYPIUM-HIRSUTUM; GENETIC DIVERSITY; RESISTANCE; GERMPLASM; NEMATODE; INTROGRESSION; CULTIVARS; REGISTRATION AB Strong resistance to reniform nematode (Rotylenchulus reniformis) was previously introgressed from the F-genome diploid species Gossypium longicalyx (2n = 2x = 26) into Upland cotton (G. hirsutum L., 2n = 4x = 52, 2[AD](1)), and attributed to the gene Ren(lon). Two resistant elite lines were released, but their seedlings are differentially stunted in nematode-infested fields. Here, we report on the development of linked SNPs and their use to disrupt "linkage drag" around Ren(lon). Using advanced backcross-inbred lines with previously identified proximal (PCO) or distal crossover (DCO) events near Ren(lon), we chose 18 Ren(lon)-linked SNP markers for mapping across two large (880) BC1F1 seed populations. Few recombinants occurred (7 of 1760). Using two of the closest Renlon-linked SNPs to select from a BC1F1 seed population (17,600), we identified 5 homeologous recombinants, but none separated the stunting and resistant phenotypes. We then compared homologous recombination rates using equally sized DCO x PCO INTERCROSS and BACKCROSS populations (n = 88). Many more recombinants occurred among progeny of the INTERCROSS (22) than the BACKCROSS (1). This finding suggests that the best SNP-based strategy for interspecific breeding with the F genome and other homeologous genomes may be to use large-scale MAS to select nearby flanking homeologous recombinants in early generations, map them to identify the two closest PCO and DCO types, then intermate them and use MAS to identify homologous recombinants. C1 [Zheng, Xiuting; Hoegenauer, Kevin A.; Hulse-Kemp, Amanda M.; Nichols, Robert L.; Stelly, David M.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Quintana, Jose; Bell, Alois A.] USDA ARS, Southern Plains Agr Res Ctr, Cotton Pathol Res Unit, 2765 F&B Rd, College Stn, TX 77845 USA. [Nichols, Robert L.] Cotton Inc, 6399 Weston Pkwy, Cary, NC 27513 USA. RP Stelly, DM (reprint author), Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. EM stelly@tamu.edu FU Cotton Incorporated Agricultural Research Cooperative Research Agreement [11-906]; Chinese Scholarship Council FX The authors gratefully acknowledge financial support by Cotton Incorporated Agricultural Research Cooperative Research Agreement 11-906, and the Chinese Scholarship Council. We thank Wayne Raska, Fei Wang and Sandria Prom for technical assistance. NR 51 TC 1 Z9 1 U1 1 U2 1 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 1526 EP 1539 DI 10.2135/cropsci2015.07.0436 PG 14 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700012 ER PT J AU Rupp, JLS Cruz, LF Trick, HN Fellers, JP AF Rupp, Jessica L. Shoup Cruz, Luisa F. Trick, Harold N. Fellers, John P. TI RNAi-Mediated, Stable Resistance to Triticum mosaic virus in Wheat SO CROP SCIENCE LA English DT Article ID DOUBLE-STRANDED-RNA; HAIRPIN-RNA; SINGLE TRANSGENE; GENE-EXPRESSION; DWARF VIRUS; CURL MITE; T-DNAS; PLANTS; VECTORS; COTRANSFORMATION AB Triticum mosaic virus (TriMV) was discovered in 2006 and affects wheat (Triticum spp.) production systems in the Great Plains of the United States. Genetic resistance is available in a few commercial varieties; however, it is not effective above 20 degrees C. RNA interference (RNAi) was evaluated as an alternative strategy to generate resistance to TriMV. An RNAi pANDA-mini-based hairpin expression vector was created from 272 bp of the TriMV coat protein (CP) sequence. Immature embryos of the wheat cultivar 'Bobwhite' were co-transformed by biolistic particle delivery system with the pANDA::TriMVCP vector and pAHC20, containing the bar gene for glufosinate selection. T-0 plants were analyzed by PCR for the presence of the RNAi TriMV CP fragment. Transgenic T-1 lines were tested for transgene expression via reverse transcription PCR Virus resistance was evaluated by mechanical inoculation with TriMV and indirect enzyme-linked immunosorbant assay (ELISA). Several lines were identified that were resistant, having little or no viral RNA. Resistant lines underwent single plant selection to the T-6 generation. Real-time PCR and ELISA showed that T-6 lines had a high level of resistance when challenged with the virus. To test if the transgene would behave similarly in an adapted variety, homozygous transgenic T-3 lines were crossed with the virus-susceptible winter wheat, 'Overley.' Real-time PCR results indicate decreased viral titer by as much as 20-fold in the T-6 transgenic lines, the F-1 crosses, and the BC1F1 lines when compared with control plants, providing evidence that this RNAi-silencing construct can provide stable resistance to TriMV and has great potential benefits to both breeders and producers. C1 [Rupp, Jessica L. Shoup; Cruz, Luisa F.; Trick, Harold N.] Kansas State Univ, Dept Plant Pathol, Throckmorton Hall, Manhattan, KS 66506 USA. [Rupp, Jessica L. Shoup] Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. [Fellers, John P.] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. RP Fellers, JP (reprint author), USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. EM john.fellers@ars.usda.gov OI Trick, Harold/0000-0001-5255-5575 FU Kansas Wheat Commission; USDA-ARS CRIS [5430-21000-005D] FX The authors would like to thank Drs. Allan Fritz and Anna Whitfield for their input on the manuscript. Also, they would like to thank Dr. Jung Hoon Lee, Julie Essig, Sheila Stevens, Hyeonju Lee, Dehlia Burdan, Bliss Benzen, and Sean Murdock for their technical assistance, and Dr. Robert Bowden for his comments. This work was supported by a grant from the Kansas Wheat Commission and USDA-ARS CRIS 5430-21000-005D. This is a joint submission of the USDA and Kansas Agricultural Experiment Station, Journal number 16-061-J. NR 46 TC 0 Z9 0 U1 7 U2 7 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 1602 EP 1610 DI 10.2135/cropsci2015.09.0577 PG 9 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700018 ER PT J AU Rosas, JE Martinez, S Bonnecarrere, V de Vida, FP Blanco, P Malosetti, M Jannink, JL Gutierrez, L AF Rosas, Juan E. Martinez, Sebastian Bonnecarrere, Victoria de Vida, Fernando Perez Blanco, Pedro Malosetti, Marcos Jannink, Jean-Luc Gutierrez, Lucia TI Comparison of Phenotyping Methods for Resistance to Stem Rot and Aggregated Sheath Spot in Rice SO CROP SCIENCE LA English DT Article ID RHIZOCTONIA-ORYZAE-SATIVAE; BLIGHT RESISTANCE; SCLEROTIUM-ORYZAE; MOLECULAR MARKERS; CALIFORNIA; CULTIVARS; PLANTS; INOCULATION; ASSOCIATION; IMPROVEMENT AB Stem and sheath diseases caused by Sclerotium oryzae Cattaneo (SCL) and Rhizoctonia oryzae-sativae Sawada Mordue (ROS) can severely reduce rice (Oryza sativa L.) yield and grain quality. Genetic resistance is the best strategy to control them. Phenotypic selection for resistance is hampered due to a heterogeneous distribution of the inoculum in the soil that generates high environmental variability and decreases genetic gain. To have higher selection accuracy it is necessary to develop phenotyping methods with high repeatability and discriminative power. Comparison of greenhouse methods have been reported for Rhizoctonia solani Kuhn, a more invasive pathogen than SCL and ROS, and for SCL, but no such comparisons are reported for ROS. Our study compares five inoculation methods for SCL and ROS to identify the more discriminant and repeatable method and to apply it for high-throughput phenotyping of hundreds of rice lines. A method that uses an agar disc with growing mycelium attached to the base of stems was found to have the best balance between discrimination among genotypes and variability among replicates of the same genotype for both pathogens. This method was used in five greenhouse experiments for phenotyping resistance to SCL and ROS in a population of 641 rice advanced breeding lines. Heritabilities of resistance ranged from 0.36 to 0.71 in these experiments. These findings have a direct application in screening for resistance of rice to SCL and ROS, and in high-throughput phenotyping for mapping loci associated to disease resistance. C1 [Rosas, Juan E.; Gutierrez, Lucia] Univ Republica, Coll Agr, Dept Stat, Garzon 780, Montevideo, Uruguay. [Rosas, Juan E.; Martinez, Sebastian; de Vida, Fernando Perez; Blanco, Pedro] Inst Nacl Invest Agr, Natl Rice Res Program, Estn Expt Treinta & Tres, Ruta 8,Km 281, Treinta 33000, Uruguay. [Rosas, Juan E.; Martinez, Sebastian; de Vida, Fernando Perez; Blanco, Pedro] Inst Nacl Invest Agr, Natl Rice Res Program, Estn Expt Treinta & Tres, Ruta 8,Km 281, Tres 33000, Uruguay. [Bonnecarrere, Victoria] Inst Nacl Invest Agr, Biotechnol Unit, Estn Expt Wilson Ferreira Aldunate, Ruta 48,Km 10, Rincon Del Colorado 90200, Canelones, Uruguay. [Malosetti, Marcos] Wageningen Univ, Biometris Appl Stat, Dept Plant Sci, Wageningen, Netherlands. [Jannink, Jean-Luc] Cornell Univ, USDA ARS, RW Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Gutierrez, Lucia] Univ Wisconsin, Dept Agron, 1575 Linden Dr, Madison, WI 53706 USA. RP Gutierrez, L (reprint author), Univ Republica, Coll Agr, Dept Stat, Garzon 780, Montevideo, Uruguay.; Gutierrez, L (reprint author), Univ Wisconsin, Dept Agron, 1575 Linden Dr, Madison, WI 53706 USA. EM gutierrezcha@wisc.edu OI Martinez Kopp, Sebastian/0000-0003-0455-5823 FU INIA [AZ-13, AZ-19]; Monsanto's Beachell-Borlaug International Scholarship Program FX This work was funded by INIA (projects AZ-13 and AZ-19). The first author was partially supported by the Monsanto's Beachell-Borlaug International Scholarship Program. Authors thank two anonymous reviewers for improving the manuscript with their suggestions. We also thank Dr. Susan McCouch for her zestful support of this work, and to the technical personnel of INIA Treinta y Tres Luis Casales, Laura Diaz, Fernando Escalante and Andrea Vergara, and undergrad student Belqui Maguna. NR 55 TC 0 Z9 0 U1 5 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 1619 EP 1627 DI 10.2135/cropsci2015.09.0598 PG 9 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700020 ER PT J AU Dia, M Wehner, TC Hassell, R Price, DS Boyhan, GE Olson, S King, S Davis, AR Tolla, GE AF Dia, Mahendra Wehner, Todd C. Hassell, Richard Price, Daniel S. Boyhan, George E. Olson, Stephen King, Stephen Davis, Angela R. Tolla, Gregory E. TI Genotype x Environment Interaction and Stability Analysis for Watermelon Fruit Yield in the United States SO CROP SCIENCE LA English DT Article ID BIPLOT ANALYSIS; GGE BIPLOT; MULTIPLICATIVE INTERACTION; SIMULTANEOUS SELECTION; STATISTICAL-ANALYSIS; PERFORMANCE TRIALS; AMMI ANALYSIS; IMPROVEMENT; CULTIVARS; QUALITY AB One of the major breeding objectives for watermelon (Citrullus lanatus [Thumb.] Matsum & Nakai) is improved fruit yield. High yielding genotypes have been identified, so we measured their stability for fruit yield and yield components over diverse environments. The objectives of this study were to (i) evaluate the yield of watermelon genotypes over years and locations, (ii) identify genotypes with high stability for yield, and (iii) measure the correlations among univariate and multivariate stability statistics. A diverse set of 40 genotypes was evaluated over 3 yr (2009, 2010, and 2011) and eight locations across the southern United States in replicated trials. Yield traits were evaluated over multiple harvests, and measured as marketable yield, fruit count, percentage cull fruit, percentage early fruit, and fruit size. There were strong effects of environment as well as genotype x environment interaction (G x E) on watermelon yield traits. Based on multiple stability measures, genotypes were classified as stable or unstable for yield. There was an advantage of hybrids over inbreds for yield components in both performance and responsiveness to favorable environments. Cultivars Big Crimson and Legacy are inbred lines with high yield and stability. A significant (P < 0.001) and positive correlation was measured for Shukla's stability variance (sigma(2)(i)), Shukla's squared hat ((s) over cap (2)(i)), Wricke's ecovalence (W-i), and deviation from regression (S-d(2)) for all the traits evaluated in this study. C1 [Dia, Mahendra; Wehner, Todd C.] North Carolina State Univ, Dept Hort Sci, Campus Box 7609, Raleigh, NC 27695 USA. [Hassell, Richard] Clemson Univ, Coastal Res & Educ Ctr, 2700 Savannah Hwy, Charleston, SC 29414 USA. [Price, Daniel S.] Georgia Cty Extens, 110 West 13th Ave,Suite C, Cordele, GA 31015 USA. [Boyhan, George E.] Univ Georgia, Dept Hort, 1111 Miller Plant Sci Bldg, Athens, GA 30602 USA. [Olson, Stephen] Univ Florida, North Florida REC, 155 Res Rd, Quincy, FL 32351 USA. [King, Stephen] Texas A&M Univ, Dept Hort Sci, 1500 Res Pkwy,Ste A120, College Stn, TX 77845 USA. [Davis, Angela R.] USDA ARS, 911 East Highway 3, Lane, OK 74555 USA. [Tolla, Gregory E.] Monsanto Seminis Veg Seeds, 37437 State Hwy 16, Woodland, CA 95695 USA. RP Wehner, TC (reprint author), North Carolina State Univ, Dept Hort Sci, Campus Box 7609, Raleigh, NC 27695 USA. EM tcwehner@gmail.com NR 56 TC 2 Z9 2 U1 3 U2 4 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 1645 EP 1661 DI 10.2135/cropsci2015.10.0625 PG 17 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700022 ER PT J AU Turner, MK Jin, Y Rouse, MN Anderson, JA AF Turner, M. Kathryn Jin, Yue Rouse, Matthew N. Anderson, James A. TI Stem Rust Resistance in 'Jagger' Winter Wheat SO CROP SCIENCE LA English DT Article ID F-SP TRITICI; DISEASE RESISTANCE; INFECTION TYPES; CONSENSUS MAP; RACE TTKS; GENE; REGISTRATION; LINES; VIRULENCE; YR17 AB 'Jagger' has been used widely as a parent to develop hard red winter wheat (Triticum aestivum L.) varieties throughout the US southern Great Plains. Jagger has resistance to the stem rust pathogen race TTTTF, which is virulent to many winter wheat cultivars, yet the genetic basis of this resistance was unknown. Marker analysis and resistance to leaf rust and stripe rust demonstrated that Jagger has the 2NS/2AS translocation from T. ventricosum (Tausch) Ces., Pass. & Gilelli. This segment contains resistance genes Sr38, Lr37, and Yr17. Stem rust infection types on Jagger, however, indicated that an additional stem rust resistance gene is present. Jagger is resistant to TTTTF whereas the Sr38 stem rust differential line 'VPM1' is susceptible. A BC1F3 population developed from the cross Jagger/2*'LMPG-6' was tested with race TTTTF. Resistant and susceptible DNA bulks were genotyped with a custom 9000 SNP Illumina iSelect Bead Chip using bulked segregant analysis. We identified a locus linked with the resistance gene on chromosome arm 4AL, where Sr7 is located. Crosses between Jagger BC1F3 lines resistant to TTTTF and germplasm with Sr7a identified no recombinants, indicating that resistance to TTTTF in Jagger could be conferred by Sr7a. We confirmed the effectiveness of Sr7a resistance to race TKTTF, which caused the stem rust epidemic in Ethiopia from 2013 to 2014. The molecular markers identified in this study may be used to screen for the resistance gene Sr7a and track its presence in breeding programs. C1 [Turner, M. Kathryn; Anderson, James A.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Turner, M. Kathryn] Land Inst Salina, Salina, KS 67401 USA. [Jin, Yue; Rouse, Matthew N.] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Jin, Yue; Rouse, Matthew N.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. RP Jin, Y (reprint author), Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA.; Jin, Y (reprint author), Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. EM yue.jin@ars.usda.gov RI Rouse, Matthew/G-5474-2011 FU USDA, Agriculture and Food Research Initiative (Triticeae Coordinated Agricultural Project) [2011-68002-30029] FX We acknowledge the contribution of Sam Gale for developing the Jagger mapping population and providing guidance and assistance with the stem rust inoculations. We thank Dr. Jim Kolmer for providing expertise for the leaf rust assays. We are also grateful to Lucy Wanschura and Kun Xiao for their help in preparing inoculum and assistance in the laboratory. Funding for this project was provided by the USDA, Agriculture and Food Research Initiative 2011-68002-30029 (Triticeae Coordinated Agricultural Project). NR 35 TC 1 Z9 1 U1 1 U2 1 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 1719 EP 1725 DI 10.2135/cropsci2015.11.0683 PG 7 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700028 ER PT J AU Dia, M Wehner, TC Hassell, R Price, DS Boyhan, GE Olson, S King, S Davis, AR Tolla, GE Bernier, J Juarez, B AF Dia, Mahendra Wehner, Todd C. Hassell, Richard Price, Daniel S. Boyhan, George E. Olson, Stephen King, Stephen Davis, Angela R. Tolla, Gregory E. Bernier, Jerome Juarez, Benito TI Value of Locations for Representing Mega-Environments and for Discriminating Yield of Watermelon in the US SO CROP SCIENCE LA English DT Article ID BIPLOT ANALYSIS; GGE BIPLOT; WIDE ADAPTATION; CULTIVARS; STABILITY; PROGRAM; CHINA; AMMI AB In a crop breeding program, multiple-location trials can be used to define target regions and mega-environments that, in turn, will help the breeder develop stable cultivars. In addition, locations can be chosen that are efficient for distinguishing among cultivars (genotypes) and that are good representatives of the target regions. The objectives of this study were to study mega-environments and identify test locations that were both discriminating and representative of target regions. Watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] fruit yield and yield components were evaluated in 3 yr and eight locations using replicated, multipleharvest trials. Data were analyzed using genotype main effect and genotype 'environment interaction (GGE) biplot model as well as other methods for stability analysis. Marketable yield and percentage early fruit had a nonrepeatable crossover pattern and thus, formed a single and complex mega-environment. Two key locations, (Kinston, NC, and Charleston, SC) were efficient representatives of two mega-environments for fruit count. Locations at Woodland, CA, and College Station, TX, can be used interchangeably for identifying genotypes with high percentage cull fruit. There was only one mega-environment for fruit size. Identification of mega-environments for watermelon in the southern United States has implications for future breeding and genotype evaluation in the United States including the use of specialized genotypes for high performance in specific locations. C1 [Dia, Mahendra; Wehner, Todd C.] North Carolina State Univ, Dept Hort Sci, Raleigh, NC 27695 USA. [Hassell, Richard] Clemson Univ, Coastal Res & Educ Ctr, 2700 Savannah Hwy, Charleston, SC 29414 USA. [Price, Daniel S.] Georgia Cty Extens, 110 West 13th Ave,Suite C, Cordele, GA 31015 USA. [Boyhan, George E.] Univ Georgia, Dept Hort, 1111 Miller Plant Sci Bldg, Athens, GA 30602 USA. [Olson, Stephen] Univ Florida, North Florida REC, 155 Res Rd, Quincy, FL 32351 USA. [King, Stephen] Texas A&M Univ, Dept Hort Sci, 1500 Res Pkwy,Ste A120, College Stn, TX 77845 USA. [Davis, Angela R.] USDA ARS, 911 East Highway 3, Lane, OK 74555 USA. [Tolla, Gregory E.; Bernier, Jerome] Monsanto Seminis Veg Seeds, 37437 State Hwy 16, Woodland, CA 95695 USA. [Juarez, Benito] 2387 McNary Way, Woodland, CA 95776 USA. RP Wehner, TC (reprint author), North Carolina State Univ, Dept Hort Sci, Raleigh, NC 27695 USA. EM tcwehner@gmail.com NR 38 TC 3 Z9 3 U1 3 U2 4 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 1726 EP 1735 DI 10.2135/cropsci2015.11.0698 PG 10 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700029 ER PT J AU Zhao, DL Gordon, VS Comstock, JC Glynn, NC Johnson, RM AF Zhao, Duli Gordon, Vanessa S. Comstock, Jack C. Glynn, Neil C. Johnson, Richard M. TI Assessment of Sugarcane Yield Potential across Large Numbers of Genotypes using Canopy Reflectance Measurements SO CROP SCIENCE LA English DT Article ID WHEAT-GRAIN-YIELD; CHLOROPHYLL CONCENTRATION; SPECTRAL REFLECTANCE; LIGHT REFLECTANCE; LEAF CHLOROPHYLL; NITROGEN; REGISTRATION; LEAVES; CORN; PREDICTION AB Canopy reflectance indices have been used to monitor plant growth and estimate yields in many field crops. Little is known if canopy reflectance of sugarcane (a complex hybrid of Saccharum spp.) can be used to estimate growth and yield potential across large numbers of genotypes (clones) in the early stages of a breeding program. The objectives of this study were to identify clonal variation in sugarcane canopy reflectance and yield components and to determine if there were any putative relationships between canopy reflectance or reflectance indices and yield variables. In Stage II of the Canal Point sugarcane breeding and cultivar development program (CP program), canopy reflectance data were collected five to six times from each of 156 to 164 randomly selected clones during the growing seasons in 2011 through 2013 using a multispectral radiometer. Normalized difference vegetation index (NDVI) and other reflectance indices were calculated using the reflectance values. Yield traits, including stalk population, mean stalk weight, cane yield, commercial recoverable sucrose (CRS), and sucrose yield, were determined on mature plants in late October. Stalk population and cane yield were most highly correlated with reflectance at the wavebands centered at 800 and 980 nm and NDVI (r = 0.50-0.68; P < 0.0001), while CRS was not related to canopy reflectance. The best time to measure canopy reflectance for yield estimation across clones was from March to April (tillering stage). Canopy reflectance measurements at the early growth stage can be used as a screening tool to estimate yield potential for large numbers of clones in sugarcane breeding programs. C1 [Zhao, Duli; Gordon, Vanessa S.; Comstock, Jack C.] USDA ARS, Sugarcane Field Stn, 12990 US Highway 441 N, Canal Point, FL 33438 USA. [Glynn, Neil C.] Syngenta Seeds Inc, 7145 58th Ave, Vero Beach, FL 32967 USA. [Johnson, Richard M.] USDA ARS, Sugarcane Res Unit, 5883 USDA Rd, Houma, LA 70360 USA. RP Zhao, DL (reprint author), USDA ARS, Sugarcane Field Stn, 12990 US Highway 441 N, Canal Point, FL 33438 USA. EM duli.zhao@ars.usda.gov NR 50 TC 0 Z9 0 U1 4 U2 4 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 1747 EP 1759 DI 10.2135/cropsci2015.12.0747 PG 13 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700031 ER PT J AU Li, GQ Xu, XY Bai, GH Carver, BF Hunger, R Bonman, JM AF Li, Genqiao Xu, Xiangyang Bai, Guihua Carver, Brett F. Hunger, Robert Bonman, J. Michael TI Identification of Novel Powdery Mildew Resistance Sources in Wheat SO CROP SCIENCE LA English DT Article ID RED WINTER-WHEAT; F-SP TRITICI; NEAR-ISOGENIC LINES; BREAD WHEAT; GENETIC DIVERSITY; MOLECULAR CHARACTERIZATION; COMMON WHEAT; PM3 LOCUS; CULTIVARS; ALLELES AB Genetic resistance plays an important role in managing powdery mildew of wheat (Triticum aestivum L.), a disease of global importance. The objectives of this study were to evaluate powdery mildew resistance and the underlying genes in US wheat breeding lines and identify novel resistance sources from a worldwide collection of winter wheat germplasm. Our results suggested that 37.5% of powdery mildew resistant breeding lines recently developed in the United States carry the Pm3a gene, indicating the abundance of Pm3a in US cultivars. Among a worldwide collection of 1,297 landraces and 874 early cultivars or breeding lines, 59 and 68 accessions showed high (HR) and moderate resistance (MR), respectively. In addition, highly resistant plants were identified in 41 heterogeneous accessions. These resistant accessions were further screened for the presence of Pm3a, Pm3b, Pm3c, Pm3d, and Pm3f genes as well as the 1RS translocation segment. Among them, 13 accessions had a Pm3 allele, and another seven accessions possessed the 1BL/1RS translocation fragment with the Pm8 gene. Genetic diversity analysis based on a set of 5,011 single-nucleotide polymorphism (SNP) markers identified genetically similar accessions. The deployment of resistance sources identified in this study in wheat breeding may greatly broaden the genetic base of powdery mildew resistance. C1 [Li, Genqiao] USDA ARS, Wheat Peanut & Other Field Crops Res Unit, Stillwater, OK 74075 USA. [Li, Genqiao] Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74075 USA. [Xu, Xiangyang] USDA ARS, Wheat Peanut & Other Field Crops Res Unit, Stillwater, OK 74078 USA. [Bai, Guihua] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Carver, Brett F.] Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74078 USA. [Hunger, Robert] Oklahoma State Univ, Dept Entomol & Plant Pathol, Stillwater, OK 74078 USA. [Bonman, J. Michael] USDA ARS, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. RP Xu, XY (reprint author), USDA ARS, Wheat Peanut & Other Field Crops Res Unit, Stillwater, OK 74078 USA. EM xiangyang.xu@ars.usda.gov FU National Research Initiative Competitive Grant (Triticeae-CAP) from the USDA National Institute of Food and Agriculture [2011-68002-30029] FX We thank Dr. Harold Bockelman of USDA-ARS National Small Grain Collection for providing germplasm used in this study, the National Research Initiative Competitive Grant 2011-68002-30029 (Triticeae-CAP) from the USDA National Institute of Food and Agriculture for providing SNP data, and Todd Lenger for his excellent technical support. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is equal opportunity provider and employer. NR 52 TC 0 Z9 0 U1 5 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 1817 EP 1830 DI 10.2135/cropsci2015.09.0551 PG 14 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700038 ER PT J AU Wise, ML Vinje, MA Conley, SP AF Wise, Mitchell L. Vinje, Marcus A. Conley, Shawn P. TI Field Application of Benzothiadiazole (BTH) to Oats (Avena sativa): Effects on Crown Rust Resistance and Avenanthramide Production SO CROP SCIENCE LA English DT Article ID F-SP AVENAE; ANTIOXIDANT ACTIVITY; CELLS; BIOSYNTHESIS; POLYPHENOLS; EXPRESSION; ELICITORS; INTEGRITY; CEREALS; ENZYME AB Avenanthramides are phenolic antioxidants produced in oat (Avena sativa L.) in response to fungal infection and, as shown in greenhouse experiments, by treatment with plant defense activators, such as benzothiadiazole (BTH). Presented here are the results of application of BTH under field conditions. Field trials were conducted in 2013 using four application rates, approximating manufacturer's recommendations, on two oat cultivars and in 2014 using three application rates on three oat cultivars. The results showed a subtle but demonstrable effect of increased avenanthramide production in vegetative tissue and mature grain and enhanced resistance to crown rust infection. Gene expression of pathogenesis related protein-1 (PR-1), phenylalanine ammonia lyase (PAL) and hydroxycinnamoyl-CoA: hydroxyanthranilate hydroxycinnamoyl-CoA transferase (HHT) was also monitored by RT-qPCR. Both PR-1 and PAL expression were demonstrably up-regulated in the field trials; HHT was not. However, greenhouse experiments showed all three genes to be elicited with 63-, 9-, and 3-fold increases, respectively. Thus, field application of plant defense activators can effectively enhance crown rust resistance and avenanthramide production in oat by up-regulating certain metabolic pathways; their practical application, however, will require substantial investigative efforts. C1 [Wise, Mitchell L.; Vinje, Marcus A.] USDA ARS, Cereal Crops Res Unit, 502 Walnut St, Madison, WI 53726 USA. [Conley, Shawn P.] Univ Wisconsin, Dept Agron, Madison, WI 53706 USA. RP Wise, ML (reprint author), USDA ARS, Cereal Crops Res Unit, 502 Walnut St, Madison, WI 53726 USA. EM mitchell.wise@ars.usda.gov NR 32 TC 0 Z9 0 U1 5 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 1904 EP 1913 DI 10.2135/cropsci2015.11.0712 PG 10 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700045 ER PT J AU Goffnett, AM Sprague, CL Mendoza, F Cichy, KA AF Goffnett, Amanda M. Sprague, Christy L. Mendoza, Fernando Cichy, Karen A. TI Preharvest Herbicide Treatments Affect Black Bean Desiccation, Yield, and Canned Bean Color SO CROP SCIENCE LA English DT Article ID PHASEOLUS-VULGARIS L.; HARVEST-AID HERBICIDES; AMMONIA-LYASE ACTIVITY; PHENOLIC-COMPOUNDS; CANNING QUALITY; EDIBLE BEANS; QTL ANALYSIS; SEED YIELD; DRY; METABOLISM AB To facilitate direct harvest, preharvest herbicide applications are used to desiccate weeds and to accelerate and promote uniform dry bean (Phaseolus vulgaris L.) maturation. Field trials were conducted near Richville, MI, in 2013 and 2014 to evaluate the effects of preharvest herbicide treatments on desiccation, yield, and canned black bean quality and color. Three black bean cultivars, Zorro, Eclipse, and Zenith, were planted on two different dates in each of 2 yr. Three preharvest herbicide treatments, paraquat, glyphosate, and saflufenacil, were applied at a standard (pods = 80% yellow) and an early (pods = 50% yellow) application timing. A nontreated control was established for each cultivar. Differences in black bean desiccation were greatest 3 d after treatment (DAT), with paraquat and saflufenacil showing the quickest desiccation at the early application timing. By 7 DAT, desiccation for most preharvest treatments was >95%. Saflufenacil applied at the early timing reduced yield by as much as 55% compared with the nontreated control. Yield was generally not affected when preharvest herbicide treatments were applied at the standard application timing. Black bean cultivar influenced canned bean color retention (Zenith > Zorro > Eclipse). Preharvest herbicide treatments reduced color retention when applied at the early application timing; glyphosate reduced color retention by as much as 24%. Because of variability in bean maturation rate within a field, preharvest herbicide treatments need to be applied as close to physiological maturity as possible to minimize yield losses and maintain color retention of canned black bean. C1 [Goffnett, Amanda M.; Sprague, Christy L.; Mendoza, Fernando] Michigan State Univ, Dept Plant Soil & Microbial Sci, 1066 Bogue St, E Lansing, MI 48824 USA. [Cichy, Karen A.] Dept Plant Soil & Microbial Sci, 1066 Bogue St, E Lansing, MI 48824 USA. [Cichy, Karen A.] USDA ARS, Sugarbeet & Bean Res Unit, 1066 Bogue St, E Lansing, MI 48824 USA. RP Sprague, CL (reprint author), Michigan State Univ, Dept Plant Soil & Microbial Sci, 1066 Bogue St, E Lansing, MI 48824 USA. EM sprague1@msu.edu NR 35 TC 0 Z9 0 U1 6 U2 6 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 1962 EP 1969 DI 10.2135/cropsci2015.08.0469 PG 8 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700050 ER PT J AU Serapiglia, MJ Boateng, AA Lee, DK Casler, MD AF Serapiglia, Michelle J. Boateng, Akwasi A. Lee, D. K. Casler, Michael D. TI Switchgrass Harvest Time Management Can Impact Biomass Yield and Nutrient Content SO CROP SCIENCE LA English DT Article ID FAST PYROLYSIS; ENERGY-PRODUCTION; REED CANARYGRASS; BIOFUEL QUALITY; FEEDSTOCK; NITROGEN; USA; ENVIRONMENTS; PRETREATMENT; SELECTION AB Switchgrass (Panicum virgatum L.) is a dedicated energy crop native to tallgrass prairie and savanna ecosystems of North America. Although high biomass yield is of significant importance for the development of switchgrass as a bioenergy crop, mineral nutrients in biomass are critically important for sustainable bioenergy crop production but are detrimental for thermochemical conversion of biomass to energy. To evaluate biomass yield and nutrient uptake or nutrient cycling in switchgrass cultivars, replicated trials across three sites (Arlington, WI; Marshfield, WI; and Urbana, IL) were established in 2009. The switchgrass cultivars were harvested once annually at upland peak, after killing frost, or post-winter in the spring from 2010 to 2014. Switchgrass biomass yield was greatest at the upland peak harvest, averaging 9.9 Mg ha(-1) with biomass decreasing as harvest was delayed. Similarly, nutrient content in the biomass was greatest at the upland peak harvest with N, P, and K at 69, 77, and 23 kg ha(-1), respectively. The majority of the N, P, and K in the biomass at the upland peak harvest was absent from the biomass at the post-frost harvest. The lower levels of nutrients and total ash in the biomass at the post-frost harvests will be beneficial for downstream conversion to bioenergy and may also reduce the future fertilizer requirements of the biomass crop. C1 [Serapiglia, Michelle J.; Boateng, Akwasi A.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Lee, D. K.] Univ Illinois, Dept Crop Sci, Coll Agr Consumer & Environm Sci, Urbana, IL 61801 USA. [Casler, Michael D.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Casler, MD (reprint author), USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. EM michael.casler@ars.usda.gov FU USDA National Institute of Food and Agriculture; Agriculture and Food Research Initiative [2011-68005-30411] FX This work was funded by the USDA National Institute of Food and Agriculture and the Agriculture and Food Research Initiative competitive grant #2011-68005-30411. The authors thank Mary Cheetham for assistance with inductively-coupled plasma analysis and acid digestion preparation. Appreciation is also extended to Jacob Karlen for his technical assistance and NIRS analysis. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer. NR 36 TC 0 Z9 0 U1 9 U2 9 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 1970 EP 1980 DI 10.2135/cropsci2015.08.0527 PG 11 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700051 ER PT J AU Reynolds, WC Miller, GL Livingston, DP Rufty, TW AF Reynolds, William Casey Miller, Grady L. Livingston, David P., III Rufty, Thomas W. TI Athletic Field Paint Color Impacts Transpiration and Canopy Temperature in Bermudagrass SO CROP SCIENCE LA English DT Article ID LIGHT SPECTRAL QUALITY; EVAPOTRANSPIRATION RATES; ST AUGUSTINEGRASS; HEAT-STRESS; WARM-SEASON; WATER-USE; TURFGRASS; GENOTYPES; NITROGEN; PHOTOSYNTHESIS AB Athletic field paints have varying impacts on turfgrass health that have been linked to their ability to alter photosynthetically active radiation and photosynthesis on the basis of color. It was further hypothesized they may also alter transpiration and canopy temperature by disrupting gas exchange at the leaf surface. Growth chamber experiments evaluated the effects of air temperature and six colors of paint on daily water loss and canopy temperature in 'Tifway' bermudagrass [Cynodon dactylon (L.) Pers. x C. transvaalensis Burtt-Davy]. Daily water loss and canopy temperature were measured every 24 h using gravimetric techniques and an infrared digital thermometer, while lab experiments examined the thickness of white and black paint on the leaf surface. In nonpainted bermudagrass canopies, daily water loss increased (P <= 0.0001) with canopy temperature from 29 to 36 degrees C, while in painted bermudagrass canopies it decreased (P <= 0.0001) as canopy temperature increased from 29 to 40 degrees C. Yellow and white paint impacted transpiration and canopy temperature the least, while black and blue caused the greatest reductions in transpiration and highest increases in canopy temperature. Cross-sections of painted Tifway indicate that paint may limit evaporative cooling by clogging stomata. Increased absorption of radiant energy by paint coupled with limited evaporative cooling result in increased heat stress and decreased turfgrass performance in painted canopies. C1 [Reynolds, William Casey] Texas A&M Univ, Soil & Crop Sci Dept, College Stn, TX 77843 USA. [Miller, Grady L.; Rufty, Thomas W.] North Carolina State Univ, Dept Crop Sci, Campus Box 7620, Raleigh, NC 27695 USA. [Livingston, David P., III] North Carolina State Univ, USDA ARS, Campus Box 7629, Raleigh, NC 27695 USA. RP Reynolds, WC (reprint author), Texas A&M Univ, Soil & Crop Sci Dept, College Stn, TX 77843 USA. EM Casey.reynolds@tamu.edu NR 24 TC 0 Z9 0 U1 3 U2 3 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 2016 EP 2025 DI 10.2135/cropsci2016.01.0028 PG 10 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700055 ER PT J AU Tracy, BF Albrecht, K Flores, J Hall, M Islam, A Jones, G Lamp, W MacAdam, JW Skinner, H Teutsch, C AF Tracy, Benjamin F. Albrecht, Ken Flores, Joao Hall, Marvin Islam, Anowarul Jones, Gordon Lamp, William MacAdam, Jennifer W. Skinner, Howard Teutsch, Chris TI Evaluation of Alfalfa-Tall Fescue Mixtures across Multiple Environments SO CROP SCIENCE LA English DT Article ID FORAGE GRASSES; DRY-MATTER; PASTURE COMMUNITIES; ESTABLISHED ALFALFA; SPECIES-DIVERSITY; BIRDSFOOT-TREFOIL; PLANT DIVERSITY; BINARY-MIXTURES; NITROGEN; LEGUMES AB Binary grass-legume mixtures can benefit forage production systems in different ways helping growers cope both with increasing input costs (e.g., N fertilizer, herbicides) and potentially more variable weather. The main objective of this study was to evaluate alfalfa (Medicago sativa L.) and tall fescue [Schedonorus phoenix (Scop.) Holub] mixtures across a wide range of environments to assess herbage accumulation, weed suppression and fertilizer nitrogen replacement values (FNRV). A common field experiment was established in 2009 and 2010 at six study sites in the United States: Maryland, Pennsylvania, Utah, Virginia, Wisconsin, and Wyoming. Experimental treatments included an alfalfa monoculture, three alfalfa-fescue mixtures with seed ratios of 75: 25, 50: 50, and 25: 75 of alfalfa/tall fescue, and tall fescue monocultures that received nitrogen applications of 0, 50, 100, 150, 200, and 300 kg N ha(-1), respectively. Data were collected over a 2-yr period. The responses of tall fescue monocultures to N fertilization differed among sites, and this contrasted with mixture yields, which did not exhibit site x treatment interactions (P > 0.05). Herbage accumulation and weed suppression were consistently higher in mixtures compared with alfalfa monocultures and tall fescue monocultures receiving less than 100 kg N ha(-1). The FNRVs for alfalfa were within the range reported for other studies and averaged 143 kg N ha(-1). The consistent herbage accumulations across these multiple environments suggest binary mixtures of alfalfa-tall fescue may be a good option for many forage-livestock producers although yields could be improved with location specific cultivar selection. C1 [Tracy, Benjamin F.; Jones, Gordon] Virginia Tech, Dept Crop & Soil Environm Sci, Blacksburg, VA 24061 USA. [Albrecht, Ken] Univ Wisconsin, Dept Agron, Madison, WI 53706 USA. [Flores, Joao] North Dakota State Univ, Carrington Res Extens Ctr, Carrington, ND 58421 USA. [Hall, Marvin] Penn State Univ, Dept Plant Sci, University Pk, PA 16802 USA. [Islam, Anowarul] Univ Wyoming, Dept Plant Sci, Laramie, WY 82071 USA. [Lamp, William] Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. [MacAdam, Jennifer W.] Utah State Univ, Dept Plants Soils & Climate, Logan, UT 84322 USA. [Skinner, Howard] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Teutsch, Chris] Virginia Tech, Dept Crop & Soil Environm Sci, Blackstone, VA 23824 USA. [Teutsch, Chris] Virginia Tech Southern Piedmont AREC, Blackstone, VA 23824 USA. RP Tracy, BF (reprint author), Virginia Tech, Dept Crop & Soil Environm Sci, Blacksburg, VA 24061 USA. EM bftracy@vt.edu NR 42 TC 1 Z9 1 U1 7 U2 7 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 2026 EP 2034 DI 10.2135/cropsci2015.09.0553 PG 9 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700056 ER PT J AU Friedrichs, MR Burton, JW Brownie, C AF Friedrichs, Martin R. Burton, Joseph W. Brownie, Cavell TI Heterosis and Genetic Variance in Soybean Recombinant Inbred Line Populations SO CROP SCIENCE LA English DT Article ID SIMILARITY MEASURES; REGISTRATION; PREDICTION; GENOME AB One of the greatest challenges in plant breeding is the selection of parental combinations that will produce progeny that are superior to either parent. The objective of this research was to determine if yield heterosis in the F-2 bulk of a soybean [Glycine max (L.) Merr.] single cross is a predictor of significant genetic variance for yield and superior line yields among random inbred lines derived from the F-2 generation. In one experiment, thirty-seven F-6:7 inbred lines from each of five bi-parental populations along with parents and the bulk F-2 generation were yield tested at three locations. Of the five populations, three had significant genotypic variance for yield. The only two transgressive segregating lines came from the population with the greatest genotypic variation for yield and the only one with mid-parent F-2 yield heterosis (15% at P > 0.06). In another experiment, random inbred lines were derived from four single crosses chosen from a seven parent half diallel cross with similar mid-parent yields. Two exhibited F-2 yield heterosis and two did not. Derived F-5:6 lines were yield tested in three environments. After adjustment for maturity, both populations derived from the non-heterotic crosses had zero genetic variation. Genetic variance estimates for the two populations derived from heterotic crosses were larger than their standard errors and significant at P > Z = 0.07 and P > Z = 0.05. These experiments provide evidence that F-2 bulk heterosis can be a predictor of significant genetic variation among inbred soybean lines derived from a single cross. C1 [Friedrichs, Martin R.] Syngenta Seeds Inc, 10290 Greenway Rd, Naples, FL 34114 USA. [Burton, Joseph W.] USDA ARS, 3127 Ligon St, Raleigh, NC 27607 USA. [Brownie, Cavell] North Carolina State Univ, 5109 SAS Hall,2311 Stinson Dr, Raleigh, NC 27695 USA. [Brownie, Cavell] North Carolina State Univ, USDA ARS, Box 7631, Raleigh, NC 27607 USA. RP Burton, JW (reprint author), USDA ARS, 3127 Ligon St, Raleigh, NC 27607 USA. EM ltta@ncsu.edu FU USDA-ARS [6645-21000-026-00D] FX We thank Dr. Thomas Carter, Jr for advice in design and review of Exp. 1. We thank Dr. Brett Carver, Dr. Jesse Gilsinger, Dr. Ramsey Lewis, Dr. Andrea Cardinal, Dr. Pedro Manjarrez-Sandoval, and Dr. Paul Murphy for useful suggestions and anonymous reviewers for their valuable critiques of this manuscript. The helpful assistance of Earl Huie, Bobby McMillen, Dr. Margarita Villagarcia, and Connie Bryant with this study is gratefully acknowledged. Thanks are also extended to the cooperation of Ricardo Goenaga, USDA-ARS TARS, Mayaguez, PR, and his staff at the soybean winter nursery, Isabella, PR. This research was funded by USDA-ARS, project no. 6645-21000-026-00D. NR 34 TC 0 Z9 0 U1 1 U2 1 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2016 VL 56 IS 4 BP 2072 EP 2079 DI 10.2135/cropsci2015.11.0702 PG 8 WC Agronomy SC Agriculture GA DX9ZZ UT WOS:000384757700061 ER PT J AU Brandl, MA Schumann, M French, BW Vidal, S AF Brandl, M. A. Schumann, M. French, B. W. Vidal, S. TI Screening of Botanical Extracts for Repellence against Western Corn Rootworm Larvae SO JOURNAL OF INSECT BEHAVIOR LA English DT Article DE Diabrotica virgifera virgifera; botanicals; repellents; turmeric; spatial analysis of distance indices ID DIABROTICA-VIRGIFERA-VIRGIFERA; CHRYSOMELIDAE LARVAE; PEST-MANAGEMENT; SWEETFLAG OIL; KILL STRATEGY; TURMERIC OIL; COUNT DATA; NEEM OIL; COLEOPTERA; INSECTICIDE AB The ability of western corn rootworm (WCR) (Diabrotica virgifera virgifera LeConte) to develop resistance to various management practices requires the development of new management options. Repellent extracts can act as efficacy enhancing agents in WCR control. The present study investigated the potential repellence of garlic (Allium sativum L.), pepper (Capsicum sp. L), cape aloe (Aloe ferox Mill.), neem (Azadirachta indica A. Juss) and turmeric (Curcuma longa L.) derived extracts against WCR larvae in a no-choice bioassays. In initial screenings garlic and turmeric were evaluated as the most promising extracts as WCR larvae avoided garlic / turmeric treated soil. Turmeric was further evaluated in a rhizotron set up to quantify larval distribution and behavioral changes over time. WCR larvae avoided turmeric treated soil up to 0.05 m from its application in the root system, resulting in an increased dispersal and the formation of multiple clusters in the rhizotron. The proportion of larvae actively moving in the soil subsequently increased, whereas larval feeding significantly decreased. The spatial and behavioral alterations in the soil lead to the conclusion that turmeric is a repellent extract for WCR larvae. Turmeric repellence might further be exploited for WCR management through a synergy with insecticidal substances or microbial antagonists. C1 [Brandl, M. A.; Schumann, M.; Vidal, S.] Univ Gottingen, Dept Crop Sci, Agr Entomol, Grisebachstr 6, D-37077 Gottingen, Germany. [French, B. W.] USDA ARS, North Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. RP Brandl, MA (reprint author), Univ Gottingen, Dept Crop Sci, Agr Entomol, Grisebachstr 6, D-37077 Gottingen, Germany. EM mbrandl1@gwdg.de FU European Union of the project INBIOSOIL [282767] FX This study was funded by means of the 7th Framework Programme of the European Union 282767 as a part of the project INBIOSOIL (http://inbiosoil.uni-goettingen.de). We would like to thank Bianca Tappe for her technical assistance, Chad Nielson for WCR eggs, and NeemBiotech Laboratory Units (Cardiff, United Kingdom) for providing the botanical extracts. We would also like to thank two anonymous reviewers who helped to improve the manuscript. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 60 TC 0 Z9 0 U1 7 U2 7 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0892-7553 EI 1572-8889 J9 J INSECT BEHAV JI J. Insect Behav. PD JUL PY 2016 VL 29 IS 4 BP 395 EP 414 DI 10.1007/s10905-016-9571-3 PG 20 WC Entomology SC Entomology GA DY1GQ UT WOS:000384843300005 ER PT J AU Windham, GL Williams, WP AF Windham, Gary L. Williams, W. Paul TI Effect of inoculum concentrations of Aspergillus flavus and A-parasiticus on aflatoxin accumulation and kernel infection in resistant and susceptible maize hybrids SO PHYTOPARASITICA LA English DT Article DE Aflatoxin; Aspergillus flavus; Aspergillus parasiticus; Corn; Fungal infection; Maize; Resistance ID GERMPLASM LINE; HEPATOCELLULAR-CARCINOMA; CORN HYBRIDS; REGISTRATION; FIELD; CONTAMINATION AB Over a 3-years period, we compared aflatoxin accumulation and kernel infection in maize hybrids inoculated with six inoculum concentrations of Aspergillus flavus isolate NRRL 3357 or A. parasiticus isolate NRRL 6111 which is a norsolorinic acid producer. Aflatoxin resistant and susceptible maize hybrids were inoculated with six conidial concentrations (ranging from 1 x 10(3) to 1 x 10(8)/ml) of either A. flavus or A. parasiticus using the side-needle technique 7 days after midsilk (50 % of the plants had silks emerged). Aspergillus kernel infection and aflatoxin contamination was determined at ca. 63 days after midsilk. For each hybrid, aflatoxin levels were very similar regardless of the inoculum concentration. With the exception of the A. parasiticus treatments in 2007, the lowest inoculum conidial concentration (1 x 10(3)/ml) for both fungal species was sufficient to produce statistical differences between the resistant and the susceptible maize hybrids for aflatoxin contamination. There was more variability in kernel infection between inoculum concentrations than that observed in aflatoxin accumulation. Since our aflatoxin evaluations are conducted in a warm, humid environment, putting less disease pressure on plants via lower inoculum concentrations may make it possible to identify genotypes that have moderate levels of resistance to aflatoxin accumulation. C1 [Windham, Gary L.; Williams, W. Paul] USDA ARS, Corn Host Plant Resistance Res Unit, Mississippi State, MS 39762 USA. RP Windham, GL (reprint author), USDA ARS, Corn Host Plant Resistance Res Unit, Mississippi State, MS 39762 USA. EM gary.windham@ars.usda.gov NR 22 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0334-2123 EI 1876-7184 J9 PHYTOPARASITICA JI Phytoparasitica PD JUL PY 2016 VL 44 IS 3 BP 333 EP 339 DI 10.1007/s12600-016-0523-4 PG 7 WC Plant Sciences SC Plant Sciences GA DX5NX UT WOS:000384428800006 ER PT J AU Ramchandran, D Hojilla-Evangelista, MP Moose, SP Rausch, KD Tumbleson, ME Singh, V AF Ramchandran, Divya Hojilla-Evangelista, Mila P. Moose, Stephen P. Rausch, Kent D. Tumbleson, M. E. Singh, Vijay TI Maize Proximate Composition and Physical Properties Correlations to Dry-Grind Ethanol Concentrations SO CEREAL CHEMISTRY LA English DT Article ID GRAIN-SORGHUM; CORN; STARCH; LOCATION; VARIETY; PROTEIN; WHEAT; YIELD AB Dry-grind ethanol plants incur economic losses because of seasonal variations in ethanol yields. One possible cause associated with ethanol yield variability is incoming grain quality. There is little published information on factors causing variation in dry-grind ethanol concentrations. The objective of this study was to determine relationships between rapidly measurable corn quality attributes (physical parameters and chemical composition) and dry-grind ethanol concentrations. Corn samples obtained from a Midwestern ethanol plant were analyzed for physical quality parameters (test weight, kernel weight, true density, percent stress cracks, and moisture content) and composition (starch, protein, oil, and soluble sugars contents) and then processed with a laboratory-scale dry-grind procedure. There were significant (P < 0.05) variations in corn quality parameters and ethanol concentrations. Correlation coefficients were significant (P < 0.05) but low (-0.50 < r < 0.50) between starch content and final ethanol concentrations (72 h) and total soluble sugar content and ethanol concentrations at 72 and 48 h. Ethanol concentrations (at 24, 48, and 72 h) were predicted as a function of a combination of grain quality factors using multiple regression methods; however, the R-2 values obtained were low. Variations in ethanol concentrations were not related to physical and chemical composition quality factors. Other factors, such as structural and physiologic attributes of corn grain, need to be evaluated. C1 [Ramchandran, Divya; Rausch, Kent D.; Tumbleson, M. E.; Singh, Vijay] Univ Illinois, Agr & Biol Engn, 1304 W Penn Ave, Urbana, IL 61801 USA. [Hojilla-Evangelista, Mila P.] ARS, Plant Polymer Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. [Moose, Stephen P.] Univ Illinois, Crop Sci, 1201 W Gregory Dr, Urbana, IL 61801 USA. RP Singh, V (reprint author), Univ Illinois, Agr & Biol Engn, 1304 W Penn Ave, Urbana, IL 61801 USA. EM vsingh@illinois.edu NR 22 TC 0 Z9 0 U1 0 U2 0 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD JUL-AUG PY 2016 VL 93 IS 4 BP 414 EP 418 DI 10.1094/CCHEM-09-15-0187-R PG 5 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DW9FX UT WOS:000383962900013 ER PT J AU Baasandorj, T Ohm, JB Simsek, S AF Baasandorj, Tsogtbayar Ohm, Jae-Bom Simsek, Senay TI Effects of Kernel Vitreousness and Protein Level on Protein Molecular Weight Distribution, Milling Quality, and Breadmaking Quality in Hard Red Spring Wheat SO CEREAL CHEMISTRY LA English DT Article ID BAKING QUALITY; SIZE; ENDOSPERM; FLOUR; TEXTURE; STARCH; BREAD AB Dark, hard, and vitreous kernel content is an important grading characteristic for hard red spring (HRS) wheat in the United States. This research investigated the associations of kernel vitreousness (KV) and protein content (PC) levels with protein molecular weight distribution (MWD), milling quality, and breadmaking quality characteristics of HRS wheat. The U.S. regional crop quality survey samples from three consecutive growing years were combined into three composite samples with different levels of wheat PC and then further segregated into separate samples with three different levels of KV. Analysis of variance showed that KV level had significant (P < 0.001) effect on variation in test weight, break flour yield, and damaged starch content. Among protein MWD parameters analyzed by size-exclusion HPLC, the high-molecular-weight polymeric proteins in the SDS-unextractable fraction had significant (P < 0.01) association with KV. Regression analysis indicated that addition of KV to the PC level improved the model for both farinograph and baking water absorption values in all three growing years. This information could help the flour milling and baking industries to further segregate HRS wheat based on KV levels in addition to PC levels for their intended end-use applications. C1 [Baasandorj, Tsogtbayar; Simsek, Senay] North Dakota State Univ, Dept Plant Sci, POB 6050,Dept 7670, Fargo, ND 58108 USA. [Ohm, Jae-Bom] North Dakota State Univ, USDA ARS, Cereal Crops Res Unit, Hard Red Spring & Durum Wheat Qual Lab, Harris Hall, Fargo, ND 58108 USA. RP Simsek, S (reprint author), North Dakota State Univ, Dept Plant Sci, POB 6050,Dept 7670, Fargo, ND 58108 USA. EM Senay.Simsek@ndsu.edu NR 31 TC 0 Z9 0 U1 1 U2 1 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD JUL-AUG PY 2016 VL 93 IS 4 BP 426 EP 434 DI 10.1094/CCHEM-09-15-0181-R PG 9 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DW9FX UT WOS:000383962900015 ER PT J AU Zhou, LW Nakasone, KK Burdsall, HH Ginns, J Vlasak, J Miettinen, O Spirin, V Niemela, T Yuan, HS He, SH Cui, BK Xing, JH Dai, YC AF Zhou, Li-Wei Nakasone, Karen K. Burdsall, Harold H., Jr. Ginns, James Vlasak, Josef Miettinen, Otto Spirin, Viacheslav Niemela, Tuomo Yuan, Hai-Sheng He, Shuang-Hui Cui, Bao-Kai Xing, Jia-Hui Dai, Yu-Cheng TI Polypore diversity in North America with an annotated checklist SO MYCOLOGICAL PROGRESS LA English DT Article DE Basidiomycota; Phylogeny; Taxonomy; Wood-decaying fungus ID RDNA SEQUENCE DATA; SP-NOV; GLOBAL DIVERSITY; PHYLOGENETIC CLASSIFICATION; COMPLEX HYMENOCHAETALES; SPECIES-DIVERSITY; PHELLINUS S.L.; INONOTUS-S.L.; ALLIED GENERA; BASIDIOMYCOTA AB Profound changes to the taxonomy and classification of polypores have occurred since the advent of molecular phylogenetics in the 1990s. The last major monograph of North American polypores was published by Gilbertson and Ryvarden in 1986-1987. In the intervening 30 years, new species, new combinations, and new records of polypores were reported from North America. As a result, an updated checklist of North American polypores is needed to reflect the polypore diversity in there. We recognize 492 species of polypores from 146 genera in North America. Of these, 232 species are unchanged from Gilbertson and Ryvarden's monograph, and 175 species required name or authority changes. In addition, 40 new species and 45 new records published since that monograph are included in the checklist. Among the 492 species of polypores, 486 species from 143 genera belong to 11 orders, while six other species from three genera have uncertain taxonomic position at the order level. Three orders, viz. Polyporales, Hymenochaetales and Russulales, accommodate most of polypore species (93.7 %) and genera (88.8 %). We hope that this updated checklist will inspire future studies in the polypore mycota of North America and contribute to the diversity and systematics of polypores worldwide. C1 [Zhou, Li-Wei; Yuan, Hai-Sheng] Chinese Acad Sci, Inst Appl Ecol, Key Lab Forest Ecol & Management, Shenyang 110164, Peoples R China. [Nakasone, Karen K.; Burdsall, Harold H., Jr.] US Forest Serv, Ctr Forest Mycol Res, Northern Res Stn, One Gifford Pinchot Dr, Madison, WI 53726 USA. [Ginns, James] 1970 Sutherland Rd, Penticton, BC V2A 8T8, Canada. [Vlasak, Josef] Acad Sci Czech Republic, Ctr Biol, Inst Plant Mol Biol, Branisovska 31, CZ-37005 Ceske Budejovice, Czech Republic. [Miettinen, Otto; Spirin, Viacheslav; Niemela, Tuomo] Univ Helsinki, Finnish Museum Nat Hist, Bot Unit, POB 7, FI-00014 Helsinki, Finland. [He, Shuang-Hui; Cui, Bao-Kai; Xing, Jia-Hui; Dai, Yu-Cheng] Beijing Forestry Univ, Inst Microbiol, POB 61, Beijing 100083, Peoples R China. RP Dai, YC (reprint author), Beijing Forestry Univ, Inst Microbiol, POB 61, Beijing 100083, Peoples R China. EM yuchengd@yahoo.com RI Vlasak, Josef/G-8083-2014; OI Niemela, Tuomo/0000-0003-1362-9038; Spirin, Viacheslav/0000-0001-5436-6997; Miettinen, Otto/0000-0001-7502-710X FU National Natural Science Foundation of China [31530002] FX Prof. David S. Hibbett (Clark University, USA) is thanked for critical suggestions. The research was financed by the National Natural Science Foundation of China (Project No. 31530002). NR 126 TC 0 Z9 0 U1 3 U2 3 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1617-416X EI 1861-8952 J9 MYCOL PROG JI Mycol. Prog. PD JUL PY 2016 VL 15 IS 7 BP 771 EP 790 DI 10.1007/s11557-016-1207-7 PG 20 WC Mycology SC Mycology GA DX4ES UT WOS:000384334600006 ER PT J AU Cai, H Wang, L Mu, W Wan, Q Wei, W Davis, RE Chen, H Zhao, Y AF Cai, H. Wang, L. Mu, W. Wan, Q. Wei, W. Davis, R. E. Chen, H. Zhao, Y. TI Multilocus genotyping of a 'Candidatus Phytoplasma aurantifolia'-related strain associated with cauliflower phyllody disease in China SO ANNALS OF APPLIED BIOLOGY LA English DT Article DE Cauliflower; multilocus genotyping; phyllody; phytoplasma; subgroup 16SrII-A ID 16S RIBOSOMAL-RNA; WITCHES-BROOM DISEASE; RFLP ANALYSIS; GENE-SEQUENCES; 1ST REPORT; YELLOWS; IDENTIFICATION; STUNT; CLASSIFICATION; 16SRII-A AB A new cauliflower disease characterised by the formation of leaf-like inflorescences and malformed flowers occurred in a seed production field located in Yunnan, a southwest province of China. Detection of phytoplasma-characteristic 16S rRNA gene sequences in DNA samples from diseased plants linked the cauliflower disease to phytoplasmal infection. Results from phylogenetic and virtual restriction fragment length polymorphism analyses of the 16S rRNA gene sequence indicated that the cauliflower-infecting agent is a 'Candidatus Phytoplasma aurantifolia'-related strain and is a new member of the peanut witches'-broom phytoplasma group, subgroup A (16SrII-A). Multilocus genotyping showed close genetic relationship between this cauliflower phytoplasma and a broad host range phytoplasma lineage found only in East Asia thus far. Molecular markers present in the secY and rp loci distinguished this phytoplasma from other members of the subgroup 16SrII-A. C1 [Cai, H.; Wan, Q.; Chen, H.] Yunnan Agr Univ, Key Lab Plant Pathol, Kunming, Peoples R China. [Wang, L.; Wan, Q.] Yuxi Normal Univ, Coll Resources & Environm, Yuxi, Peoples R China. [Mu, W.] Yunnan Acad Agr Sci, Inst Trop Ecoagr Sci, Yuanmou, Peoples R China. [Wei, W.; Davis, R. E.; Zhao, Y.] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. RP Zhao, Y (reprint author), USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. EM yan.zhao@ars.usda.gov FU Program for Innovative Research Team (in Science and Technology) in University of Yunnan Province, IRTSTYN; National Natural Science Foundation of China [31060239]; US Department of Agriculture, Agricultural Research Service [1245-22000-282-00D] FX This research was supported by the Program for Innovative Research Team (in Science and Technology) in University of Yunnan Province, IRTSTYN (2014-2022), the National Natural Science Foundation of China (grant 31060239) and the US Department of Agriculture, Agricultural Research Service (Project number 1245-22000-282-00D). NR 55 TC 1 Z9 1 U1 3 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0003-4746 EI 1744-7348 J9 ANN APPL BIOL JI Ann. Appl. Biol. PD JUL PY 2016 VL 169 IS 1 BP 64 EP 74 DI 10.1111/aab.12281 PG 11 WC Agriculture, Multidisciplinary SC Agriculture GA DW0YV UT WOS:000383370600007 ER PT J AU Carvalho, CR Wedge, DE Cantrell, CL Silva-Hughes, AF Pan, ZQ Moraes, RM Madoxx, VL Rosa, LH AF Carvalho, Camila R. Wedge, David E. Cantrell, Charles L. Silva-Hughes, Alice F. Pan, Zhiqiang Moraes, Rita M. Madoxx, Victor L. Rosa, Luiz H. TI Molecular Phylogeny, Diversity, and Bioprospecting of Endophytic Fungi Associated with wild Ethnomedicinal North American Plant Echinacea purpurea (Asteraceae) SO CHEMISTRY & BIODIVERSITY LA English DT Article DE Echinacea purpurea; Endophytic fungi; Symbiosis; Bioactive compounds; Dihydroisocoumarin ID FATTY-ACIDS; ANTIFUNGAL ACTIVITY; BISCOGNIAUXIA-MEDITERRANEA; TISSUE; DIHYDROISOCOUMARINS; IDENTIFICATION; EXTRACTS; CULTURES; DNA; OAK AB The endophytic fungal community associated with the ethnomedicinal plant Echinacea purpurea was investigated as well as its potential for providing antifungal compounds against plant pathogenic fungi. A total of 233 endophytic fungal isolates were obtained and classified into 42 different taxa of 16 genera, of which Alternaria alternata, Colletotrichum dematium, and Stagonosporopsis sp. 2 are the most frequent colonizers. The extracts of 29 endophytic fungi displayed activities against important phytopathogenic fungi. Eight antifungal extracts were selected for chemical analysis. Forty fatty acids were identified by gas chromatography-flame-ionization detection (GC-FID) analysis. The compounds (-)-5-methylmellein and (-)(3R)-8-hydroxy-6-methoxy-3,5-dimethyl-3,4-dihydroisocoumarin were isolated from Biscogniauxia mediterranea EPU38CA crude extract. (-)-5-Methylmellein showed weak activity against Phomopsis obscurans, P. viticola, and Fusarium oxysporum, and caused growth stimulation of C. fragariae, C. acutatum, C. gloeosporioides, and Botrytis cinerea. (-)-(3R)-8-Hydroxy-6-methoxy-3,5-dimethyl-3,4-dihydroisocoumarin appeared slightly more active in the microtiter environment than 5-methylmellein. Our results indicate that E. purpurea lives symbiotically with different endophytic fungi, which are able to produce bioactive fatty acids and aromatic compounds active against important phytopathogenic fungi. The detection of the different fatty acids and aromatic compounds produced by the endophytic community associated with wild E. purpurea suggests that it may have intrinsic mutualistic resistance against phytopathogen attacks in its natural environment. C1 [Carvalho, Camila R.; Silva-Hughes, Alice F.; Rosa, Luiz H.] Univ Fed Minas Gerais, Inst Biol Sci, Dept Microbiol, POB 486, BR-31270901 Belo Horizonte, MG, Brazil. [Wedge, David E.; Cantrell, Charles L.; Pan, Zhiqiang] ARS, Nat Prod Utilizat Res Unit, USDA, Oxford, MS 38677 USA. [Moraes, Rita M.] Univ Mississippi, Sch Pharm, Natl Ctr Nat Prod Res, Oxford, MS 38677 USA. [Moraes, Rita M.] Univ Mississippi, Ctr Water & Wetland Resources, Abbeville, MS 38601 USA. [Madoxx, Victor L.] Mississippi State Univ, Geosyst Res Inst, Starkville, MS 39762 USA. RP Rosa, LH (reprint author), Univ Fed Minas Gerais, Inst Biol Sci, Dept Microbiol, POB 486, BR-31270901 Belo Horizonte, MG, Brazil. EM lhrosa@icb.ufmg.br FU FAPEMIG; CAPES [23038.003478/2013-92]; FAPEMIG [0050-13]; CBB [22007, 12]; National Center for Natural Products Research, University of Mississippi through from the USDA, Agricultural Research Service [58-6408-2-0009] FX Visiting Graduate Scholar of Camila R. Carvalho was financially supported by FAPEMIG. We are grateful for financial support from CAPES (23038.003478/2013-92), FAPEMIG (0050-13), and CBB (22007; 12). The National Center for Natural Products Research, University of Mississippi is partially supported through a cooperative scientific agreement 58-6408-2-0009 from the USDA, Agricultural Research Service. The authors thank Mrs. Ramona K. Pace, Ms. Linda J. Robertson, Mrs. Amber Reichley, Mr. Solomon Green III, and Mrs. Marilyn Ruscoe for technical support. NR 43 TC 0 Z9 0 U1 7 U2 7 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA POSTFACH 101161, 69451 WEINHEIM, GERMANY SN 1612-1872 EI 1612-1880 J9 CHEM BIODIVERS JI Chem. Biodivers. PD JUL PY 2016 VL 13 IS 7 BP 918 EP 930 DI 10.1002/cbdv.201500299 PG 13 WC Biochemistry & Molecular Biology; Chemistry, Multidisciplinary SC Biochemistry & Molecular Biology; Chemistry GA DW1HE UT WOS:000383393100014 PM 27273012 ER PT J AU Baker, TC Zhu, JWJ Millar, JG AF Baker, Thomas C. Zhu, Junwei J. Millar, Jocelyn G. TI Delivering on the Promise of Pheromones PREFACE SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Editorial Material ID ODORANT RECEPTORS; SEX-PHEROMONES; FAMILY C1 [Baker, Thomas C.] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. [Zhu, Junwei J.] ARS, USDA, Agroecosyst Management Res Unit, Lincoln, NE USA. [Millar, Jocelyn G.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Millar, Jocelyn G.] Univ Calif Riverside, Dept Chem, Riverside, CA 92521 USA. RP Baker, TC (reprint author), Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. EM tcb10@psu.edu NR 6 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 EI 1573-1561 J9 J CHEM ECOL JI J. Chem. Ecol. PD JUL PY 2016 VL 42 IS 7 BP 553 EP 556 DI 10.1007/s10886-016-0744-5 PG 4 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA DV7ES UT WOS:000383099600001 PM 27553011 ER PT J AU Cui, GZ Zhu, JJ AF Cui, Gen Zhong Zhu, Junwei Jerry TI Pheromone-Based Pest Management in China: Past, Present, and Future Prospects SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Sex pheromone; Mass trapping; Mating disruption; Marketing; Regulation; Pest management ID CODLING MOTH LEPIDOPTERA; NILAPARVATA-LUGENS EGGS; SEX-PHEROMONE; BOMBYX-MORI; INSECT PHEROMONE; MOLECULAR CHARACTERIZATION; ATTRACTION-INHIBITORS; ANAGRUS-NILAPARVATAE; VOLATILE EMISSIONS; PLANT-RESISTANCE AB Semiochemical-based pest management technology has been widely used to monitor and control insect pests in agricultural, forestry, and public health sectors in the western world. It became a popular tool in the early 1970s with tremendous efforts in developing environment-friendly control technologies for the integrated pest management. However, in China, similar research lagged 15 to 20 years and was not initiated until the late 1980s. In this review, we present the early history of pheromone research that has led to the current practical applications in China, particularly in the development of pheromone-based pest management products. We also provide information regarding the current status of pheromone-based product manufacturing, marketing, and regulatory issues related to local semiochemical industries, which may be useful to other international companies interested in pursuing business in China. In addition, we share some research topics that represent new directions of the present pheromone research to explore novel tools for advancing semiochemical-based pest management in China. C1 [Cui, Gen Zhong] Pherobio Technol Co Ltd, Beijing, Peoples R China. [Zhu, Junwei Jerry] ARS, USDA, Agroecosyst Management Res Unit, UNL EC, 305B Entomol Hall, Lincoln, NE 68583 USA. RP Zhu, JJ (reprint author), ARS, USDA, Agroecosyst Management Res Unit, UNL EC, 305B Entomol Hall, Lincoln, NE 68583 USA. EM jerry.zhu@ars.usda.gov NR 139 TC 1 Z9 1 U1 8 U2 8 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 EI 1573-1561 J9 J CHEM ECOL JI J. Chem. Ecol. PD JUL PY 2016 VL 42 IS 7 BP 557 EP 570 DI 10.1007/s10886-016-0731-x PG 14 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA DV7ES UT WOS:000383099600002 PM 27481347 ER PT J AU Lance, DR Leonard, DS Mastro, VC Walters, ML AF Lance, David R. Leonard, Donna S. Mastro, Victor C. Walters, Michelle L. TI Mating Disruption as a Suppression Tactic in Programs Targeting Regulated Lepidopteran Pests in US SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Review DE Eradication; Containment; Gypsy moth; Pink bollworm; Light brown apple moth; European grapevine moth; Aerial application; Disparlure; Slow the spread; Invasive pests ID BROWN APPLE MOTH; EPIPHYAS-POSTVITTANA LEPIDOPTERA; SEX-PHEROMONE BLEND; GYPSY-MOTH; LOBESIA-BOTRANA; PINK-BOLLWORM; GRAPEVINE MOTH; COMMUNICATION DISRUPTION; SCALE TRIALS; NEW-ZEALAND AB Mating disruption, the broadcast application of sex-attractant pheromone to reduce the ability of insects to locate mates, has proven to be an effective method for suppressing populations of numerous moth pests. Since the conception of mating disruption, the species-specificity and low toxicity of pheromone applications has led to their consideration for use in area-wide programs to manage invasive moths. Case histories are presented for four such programs where the tactic was used in the United States: Pectinophora gossypiella (pink bollworm), Lymantria dispar (gypsy moth), Epiphyas postvittana (light brown apple moth), and Lobesia botrana (European grapevine moth). Use of mating disruption against P. gossypiella and L. botrana was restricted primarily to agricultural areas and relied in part (P. gossypiella) or wholly (L. botrana) on hand-applied dispensers. In those programs, mating disruption was integrated with other suppression tactics and considered an important component of overall efforts that are leading toward eradication of the invasive pests from North America. By contrast, L. dispar and E. postvittana are polyphagous pests, where pheromone formulations have been applied aerially as stand-alone treatments across broad areas, including residential neighborhoods. For L. dispar, mating disruption has been a key component in the program to slow the spread of the infestation of this pest, and the applications generally have been well tolerated by the public. For E. postvittana, public outcry halted the use of aerially applied mating disruption after an initial series of treatments, effectively thwarting an attempt to eradicate this pest from California. Reasons for the discrepancies between these two programs are not entirely clear. C1 [Lance, David R.; Mastro, Victor C.] USDA APHIS PPQ, CPHST Otis Lab, 1398 W Truck Rd, Buzzards Bay, MA 02542 USA. [Leonard, Donna S.] US Forest Serv, USDA, Southern Reg, Forest Hlth Protect, 200 WT Weaver Blvd, Asheville, NC 28804 USA. [Walters, Michelle L.] USDA APHIS PPQ, CPHST Phoenix Lab, 3645 E Wier Ave, Phoenix, AZ 85040 USA. RP Lance, DR (reprint author), USDA APHIS PPQ, CPHST Otis Lab, 1398 W Truck Rd, Buzzards Bay, MA 02542 USA. EM david.r.lance@aphis.usda.gov NR 135 TC 0 Z9 0 U1 9 U2 9 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 EI 1573-1561 J9 J CHEM ECOL JI J. Chem. Ecol. PD JUL PY 2016 VL 42 IS 7 BP 590 EP 605 DI 10.1007/s10886-016-0732-9 PG 16 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA DV7ES UT WOS:000383099600005 PM 27492468 ER PT J AU Landolt, P Zhang, QH AF Landolt, Peter Zhang, Qing-He TI Discovery and Development of Chemical Attractants Used to Trap Pestiferous Social Wasps (Hymenoptera: Vespidae) SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Social wasp; Vespidae; Vespula; Dolichovespula; Vespa; Polistes; Acetic acid; Isobutanol; 2-Methyl-1-butanol; Heptyl butyrate; Attractant; Trap ID VESPULA-GERMANICA HYMENOPTERA; CONTROLLED-RELEASE DISPENSERS; NORTH-AMERICA HYMENOPTERA; YELLOW JACKETS VESPULA; XESTIA-C-NIGRUM; ACETIC-ACID; HEPTYL BUTYRATE; MAMESTRA-CONFIGURATA; LACANOBIA-SUBJUNCTA; FRUIT AB Chemical attractants for trapping temperate social wasps have been discovered during the screening of chemicals as attractants for flies, the study of pentatomid bug pheromones, and the testing of volatiles of fermented sweet baits. Wasp attraction to these chemicals seems to be related to either food-finding or prey-finding behavior. Of these attractive chemicals, commercial lures marketed in North America for trapping wasps generally contain heptyl butyrate, or the combination of acetic acid and 2-methyl-1-butanol. Heptyl butyrate is a very good attractant for two major pest wasp species in North America and minor wasp pests in the Vespula rufa species group. The combination of acetic acid with isobutanol attracted nearly all North American pest species of social wasps, including yellowjackets (Vespula and Dolichovespula), a hornet (Vespa crabro), and several paper wasps (Polistes spp.). The testing of wasp chemical attractants in different geographic areas demonstrated responses of many wasp taxa and showed a broad potential scope for the marketing of trap lures. Comparisons of compounds structurally similar to isobutanol revealed similar activity with 2-methyl-1-butanol, which is now used commercially because of a vapor pressure that is more favorable than isobutanol for formulations and dispensers. Doses and concentrations needed for good wasp catches were determined for heptyl butyrate, acetic acid, isobutanol, and 2-methyl-1-butanol, either formulated in water or dispensed from a controlled release device. Trap designs were developed based on consumer considerations; visual appeal, ease and safety of use, and low environmental impact. The resultant lures and traps are marketed in numerous physical and on-line retail outlets throughout the United States and southern Canada. C1 [Landolt, Peter] ARS, USDA, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. [Zhang, Qing-He] Sterling Int Inc, 3808 N Sullivan Rd,Bldg 16, Spokane, WA 99216 USA. RP Landolt, P (reprint author), ARS, USDA, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM peter.landolt@ars.usda.gov NR 80 TC 0 Z9 0 U1 15 U2 15 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 EI 1573-1561 J9 J CHEM ECOL JI J. Chem. Ecol. PD JUL PY 2016 VL 42 IS 7 BP 655 EP 665 DI 10.1007/s10886-016-0721-z PG 11 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA DV7ES UT WOS:000383099600010 PM 27435228 ER PT J AU Hakk, H Shelver, WL Casey, FXM AF Hakk, Heldur Shelver, Weilin L. Casey, Francis X. M. TI Fate and transport of the beta-adrenergic agonist ractopamine hydrochloride in soil-water systems SO JOURNAL OF ENVIRONMENTAL SCIENCES LA English DT Article DE Ractopamine; Transport; Degradation; beta-Adrenergic agonist; Soil ID AGRICULTURAL SOILS; 17-BETA-ESTRADIOL; METABOLITES; SORPTION; MANURE; IDENTIFICATION; TESTOSTERONE; DISPOSITION; PERSISTENCE; RESIDUES AB The feed additive ractopamine hydrochloride was fortified at four concentrations into batch vials containing soils that differed in both biological activity and organic matter (OM). Sampling of the liquid layer for 14 days demonstrated that ractopamine rapidly dissipated from the liquid layer. Less than 20% of the fortified dose remained in the liquid layer after 4 hr, and recoveries of dosed ractopamine ranged from 8 to 18% in the liquid layer at 336 hr. Sorption to soil was the major fate for ractopamine in soil: water systems, i.e., 42%-51% of the dose at 14 days. The major portion of the sorbed fraction was comprised of non-extractables; a smaller fraction of the sorbed dose was extracted into water and acetone, portions which would be potentially mobile in the environment. Partitioning coefficients for all soils suggested strong sorption of ractopamine to soil which is governed by hydrophobic interactions and cation exchange complexes within the soil OM. Ractopamine degradation was observed, but to mostly non-polar compounds which had a higher potential than ractopamine to sorb to soil. The formation of volatiles was also suggested. Therefore, despite rapid and extensive soil sorption, these studies indicated a portion of ractopamine, present in manures used to fertilize soils, may be mobile in the environment via water-borne events. (C) 2016 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. C1 [Hakk, Heldur; Shelver, Weilin L.] USDA ARS, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58102 USA. [Casey, Francis X. M.] North Dakota State Univ, Dept Soil Sci, Albrecht Blvd, Fargo, ND 58108 USA. RP Hakk, H (reprint author), USDA ARS, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58102 USA. EM heldur.hakk@ars.usda.gov RI Casey, Francis/A-2135-2010 OI Casey, Francis/0000-0002-6035-7234 NR 38 TC 0 Z9 0 U1 1 U2 1 PU SCIENCE PRESS PI BEIJING PA 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA SN 1001-0742 EI 1878-7320 J9 J ENVIRON SCI-CHINA JI J. Environ. Sci. PD JUL PY 2016 VL 45 BP 40 EP 48 DI 10.1016/j.jes.2015.11.026 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DW6TP UT WOS:000383784400005 PM 27372117 ER PT J AU Jurjevic, Z Peterson, SW AF Jurjevic, Zeljko Peterson, Stephen W. TI Aspergillus asper sp nov and Aspergillus collinsii sp nov., from Aspergillus section Usti SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID PHYLOGENETIC ANALYSIS; DNA-SEQUENCES; PENICILLIUM; INFECTIONS AB In sampling fungi from the built environment, two isolates that could not confidently be placed in described species were encountered. Phenotypic analysis suggested that they belonged in Aspergillus sect. Usti. In order to verify the sectional placement and to assure that they were undescribed rather than phenotypically aberrant isolates, DNA was isolated and sequenced at the beta-tubulin, calmodulin, internal transcribed spacer and RNA polymerase II loci and sequences compared with those from other species in the genus Aspergillus. At each locus, each new isolate was distant from existing species. Phylogenetic trees calculated from these data and GenBank data for species of the section Usti excluded the placement of these isolates in existing species, with statistical support. Because they were excluded from existing taxa, the distinct species Aspergillus asper (type strain NRRL 35910(T)) and Aspergillus collinsii (type strain NRRL 66196(T)) in sect. Usti are proposed to accommodate these strains. C1 [Jurjevic, Zeljko] EMSL Analyt Inc, 200 North Route 130, Cinnaminson, NJ 08077 USA. [Peterson, Stephen W.] ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 North Univ St, Peoria, IL 61604 USA. RP Peterson, SW (reprint author), ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 North Univ St, Peoria, IL 61604 USA. EM stephen.peterson@ars.usda.gov NR 22 TC 0 Z9 0 U1 5 U2 5 PU MICROBIOLOGY SOC PI LONDON PA CHARLES DARWIN HOUSE, 12 ROGER ST, LONDON WC1N 2JU, ERKS, ENGLAND SN 1466-5026 EI 1466-5034 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD JUL PY 2016 VL 66 BP 2566 EP 2572 DI 10.1099/ijsem.0.001094 PN 7 PG 7 WC Microbiology SC Microbiology GA DV4ON UT WOS:000382905000016 ER PT J AU Rajao, DS Chen, HJ Perez, DR Sandbulte, MR Gauger, PC Loving, CL Shanks, GD Vincent, A AF Rajao, Daniela S. Chen, Hongjun Perez, Daniel R. Sandbulte, Matthew R. Gauger, Phillip C. Loving, Crystal L. Shanks, G. Dennis Vincent, Amy TI Vaccine-associated enhanced respiratory disease is influenced by haemagglutinin and neuraminidase in whole inactivated influenza virus vaccines SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID DEPENDENT CELLULAR CYTOTOXICITY; A VIRUS; LINKED LECTIN; ANTIBODY; SWINE; PIGS; PROTECTION; INFECTION; ANTIGEN; HUMANS AB Multiple subtypes and many antigenic variants of influenza A virus (IAV) co-circulate in swine in the USA, complicating effective use of commercial vaccines to control disease and transmission. Whole inactivated virus (WIV) vaccines may provide partial protection against IAV with substantial antigenic drift, but have been shown to induce vaccine-associated enhanced respiratory disease (VAERD) when challenged with an antigenic variant of the same haemagglutinin (HA) subtype. This study investigated the role the immune response against HA, neuraminidase (NA) and nucleoprotein (NP) may play in VAERD by reverse engineering vaccine and challenge viruses on a common backbone and using them in a series of vaccination/challenge trials. Mismatched HA between vaccine and challenge virus was necessary to induce VAERD. However, vaccines containing a matched NA abrogated the VAERD phenomenon induced by the HA mismatch and this was correlated with NA-inhibiting (NI) antibodies. Divergence between the two circulating swine N2 lineages (92% identity) resulted in a loss of NI cross-reactivity and also resulted in VAERD with the mismatched HA. The NP lineage selected for use in the WIV vaccine strains did not affect protection or pathology. Thus the combination of HA and NA in the vaccine virus strains played a substantial role in vaccine protection versus immunopathology, suggesting that vaccines that target the HA protein alone could be more prone to VAERD due to the absence of cross-protective NI antibodies. C1 [Rajao, Daniela S.; Loving, Crystal L.; Vincent, Amy] ARS, Virus & Prion Dis Livestock Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. [Chen, Hongjun; Perez, Daniel R.] Univ Maryland, Dept Vet Med, College Pk, MD 20742 USA. [Chen, Hongjun; Perez, Daniel R.] Virginia Maryland Reg Coll Vet Med, College Pk, MD USA. [Sandbulte, Matthew R.] Iowa State Univ, Dept Vet Microbiol & Prevent Med, Ames, IA USA. [Gauger, Phillip C.] Iowa State Univ, Dept Vet Diagnost & Prod Anim Med, Ames, IA USA. [Shanks, G. Dennis] Australian Army Malaria Inst, Enoggera, Qld, Australia. [Perez, Daniel R.] Univ Georgia, Poultry Diagnost & Res Ctr, Athens, GA 30602 USA. [Sandbulte, Matthew R.] Merck Anim Hlth, 35500 W 91st St, Desoto, KS 66018 USA. RP Vincent, A (reprint author), ARS, Virus & Prion Dis Livestock Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. EM amy.vincent@ars.usda.gov OI de Souza Rajao, Daniela/0000-0002-0772-0065 FU USDA-ARS; USDA-APHIS; DOD-AFHSB; CNPq-Brazil FX We thank Michelle Harland, Gwen Nordholm, Zahra Olson and Katie Gibson for technical assistance; Jason Huegel, Jason Crabtree and Tyler Standley for assistance with animal studies; and Dr Susan Brockmeier for assistance with microbiologic screening. We also thank Dr John Brundage for his helpful insight at all stages of these studies. This study was supported by USDA-ARS, USDA-APHIS and DOD-AFHSB. D. S. R. was a CNPq-Brazil fellowship recipient. USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The opinions expressed are those of the authors and do not necessarily reflect those of the Australian Defence Force or the US Department of Defense. NR 45 TC 0 Z9 0 U1 3 U2 3 PU MICROBIOLOGY SOC PI LONDON PA CHARLES DARWIN HOUSE, 12 ROGER ST, LONDON WC1N 2JU, ERKS, ENGLAND SN 0022-1317 EI 1465-2099 J9 J GEN VIROL JI J. Gen. Virol. PD JUL PY 2016 VL 97 BP 1489 EP 1499 DI 10.1099/jgv.0.000468 PN 7 PG 11 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA DV4OP UT WOS:000382905200001 PM 27031847 ER PT J AU Burmakina, G Malogolovkin, A Tulman, ER Zsak, L Delhon, G Diel, DG Shobogorov, NM Morgunov, YP Morgunov, SY Kutish, GF Kolbasov, D Rock, DL AF Burmakina, G. Malogolovkin, A. Tulman, E. R. Zsak, L. Delhon, G. Diel, D. G. Shobogorov, N. M. Morgunov, Yu P. Morgunov, S. Yu Kutish, G. F. Kolbasov, D. Rock, D. L. TI African swine fever virus serotype-specific proteins are significant protective antigens for African swine fever SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID IMMUNE-RESPONSE; VIRULENCE; GENE; HEMAGGLUTININ; IMMUNIZATION; MACROPHAGES; CHALLENGE; GENOTYPES; STRAINS; INVITRO AB African swine fever (ASF) is an emerging disease threat for the swine industry worldwide. No ASF vaccine is available and progress is hindered by lack of knowledge concerning the extent of ASFV strain diversity and the viral antigens conferring type-specific protective immunity in pigs. Available data from vaccination/challenge experiments in pigs indicate that ASF protective immunity may be haemadsorption inhibition (HAI) serotype-specific. Recently, we have shown that two ASFV proteins, CD2v (EP402R) and C-type lectin (EP153R), are necessary and sufficient for mediating HAI serological specificity (Malogolovkin et al., 2015).. Here, using ASFV inter-serotypic chimeric viruses and vaccination/challenge experiments in pigs, we demonstrate that serotype-specific CD2v and/or C-type lectin proteins are important for protection against homologous ASFV infection. Thus, these viral proteins represent significant protective antigens for ASFV that should be targeted in future vaccine design and development. Additionally, these data support the concept of HAI serotype-specific protective immunity. C1 [Burmakina, G.; Malogolovkin, A.; Shobogorov, N. M.; Morgunov, Yu P.; Morgunov, S. Yu; Kolbasov, D.] Russian Acad Agr Sci, Natl Res Inst Vet Virol & Microbiol, Pokrov, Russia. [Tulman, E. R.; Kutish, G. F.] Univ Connecticut, Dept Pathobiol & Vet Sci, Storrs, CT USA. [Tulman, E. R.; Kutish, G. F.] Univ Connecticut, Ctr Excellence Vaccine Res, Storrs, CT USA. [Zsak, L.] ARS, USDA, Southeast Poultry Res Lab, Athens, GA USA. [Delhon, G.] Univ Nebraska, Sch Vet & Biomed Sci, Lincoln, NE USA. [Delhon, G.] Univ Nebraska, Nebraska Ctr Virol, Lincoln, NE USA. [Diel, D. G.; Rock, D. L.] Univ Illinois, Coll Vet Med, Dept Pathobiol, Urbana, IL 61801 USA. RP Kolbasov, D (reprint author), Russian Acad Agr Sci, Natl Res Inst Vet Virol & Microbiol, Pokrov, Russia.; Rock, DL (reprint author), Univ Illinois, Coll Vet Med, Dept Pathobiol, Urbana, IL 61801 USA. EM kolbasov.denis@gmail.com; dlrock@illinois.edu FU Russian Science Foundation [16-16-00090]; National Pork Board [13-102]; USDA National Institute of Food and Agriculture [2013-67015-21335] FX This project was supported by the Russian Science Foundation (grant 16-16-00090), the National Pork Board (grant 13-102) and the USDA National Institute of Food and Agriculture (grant 2013-67015-21335). NR 42 TC 0 Z9 0 U1 1 U2 1 PU MICROBIOLOGY SOC PI LONDON PA CHARLES DARWIN HOUSE, 12 ROGER ST, LONDON WC1N 2JU, ERKS, ENGLAND SN 0022-1317 EI 1465-2099 J9 J GEN VIROL JI J. Gen. Virol. PD JUL PY 2016 VL 97 BP 1670 EP 1675 DI 10.1099/jgv.0.000490 PN 7 PG 6 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA DV4OP UT WOS:000382905200021 PM 27114233 ER PT J AU Haley, NJ Siepker, C Greenlee, JJ Richt, JA AF Haley, Nicholas J. Siepker, Christopher Greenlee, Justin J. Richt, Jurgen A. TI Limited amplification of chronic wasting disease prions in the peripheral tissues of intracerebrally inoculated cattle SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID BOVINE SPONGIFORM ENCEPHALOPATHY; QUAKING-INDUCED CONVERSION; DEER ODOCOILEUS-HEMIONUS; WHITE-TAILED DEER; MULE DEER; SCRAPIE PRION; AGENT; INFECTIVITY; SUSCEPTIBILITY; TRANSMISSION AB Chronic wasting disease (CWD) is a fatal neurodegenerative disease, classified as a prion disease or transmissible spongiform encephalopathy (TSE) similar to bovine spongiform encephalopathy (BSE). Cervids affected by CWD accumulate an abnormal protease-resistant prion protein throughout the central nervous system (CNS), as well as in both lymphatic and excretory tissues an aspect of prion disease pathogenesis not observed in cattle with BSE. Using seeded amplification through real-time quaking-induced conversion, we investigated whether the bovine host or prion agent was responsible for this aspect of TSE pathogenesis. We blindly examined numerous central and peripheral tissues from cattle inoculated with CWD for prion seeding activity. Seeded amplification was readily detected in the CNS, though rarely observed in peripheral tissues, with a limited distribution similar to that of BSE prions in cattle. This seems to indicate that prion peripheralization in cattle is a host-driven characteristic of TSE infection. C1 [Haley, Nicholas J.; Siepker, Christopher; Richt, Jurgen A.] Kansas State Univ, Coll Vet Med, Dept Diagnost Med & Pathobiol, Manhattan, KS 66506 USA. [Haley, Nicholas J.] Midwestern Univ, Dept Basic Sci, Glendale, AZ USA. [Greenlee, Justin J.] ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. [Haley, Nicholas J.] Midwestern Univ, Dept Microbiol & Immunol, Glendale, AZ USA. RP Haley, NJ (reprint author), Kansas State Univ, Coll Vet Med, Dept Diagnost Med & Pathobiol, Manhattan, KS 66506 USA.; Haley, NJ (reprint author), Midwestern Univ, Dept Basic Sci, Glendale, AZ USA.; Haley, NJ (reprint author), Midwestern Univ, Dept Microbiol & Immunol, Glendale, AZ USA. EM nicholas.j.haley@gmail.com NR 24 TC 0 Z9 0 U1 6 U2 6 PU MICROBIOLOGY SOC PI LONDON PA CHARLES DARWIN HOUSE, 12 ROGER ST, LONDON WC1N 2JU, ERKS, ENGLAND SN 0022-1317 EI 1465-2099 J9 J GEN VIROL JI J. Gen. Virol. PD JUL PY 2016 VL 97 BP 1720 EP 1724 DI 10.1099/jgv.0.000438 PN 7 PG 5 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA DV4OP UT WOS:000382905200027 PM 27031704 ER PT J AU Hobbie, EA Rice, SF Weber, NS Smith, JE AF Hobbie, Erik A. Rice, Samuel F. Weber, Nancy S. Smith, Jane E. TI Isotopic evidence indicates saprotrophy in post-fire Morchella in Oregon and Alaska SO MYCOLOGIA LA English DT Article DE carbon sources; ectomycorrhizal; life history; post-fire; saprotrophy ID TRUE MORELS MORCHELLA; UNITED-STATES; PICEA-ABIES; FOREST; CARBON; FUNGI; ECTOMYCORRHIZAL; ASSOCIATION; GROWTH; ROOTS AB We assessed the nutritional strategy of true morels (genus Morchella) collected in 2003 and 2004 in Oregon and Alaska, 1 or 2 y after forest fires. We hypothesized that the patterns of stable isotopes (delta C-13 and delta N-15) in the sporocarps would match those of saprotrophic fungi and that radiocarbon (Delta C-14) analyses would indicate that Morchella was assimilating old carbon not current-year photosynthate. We compared radiocarbon and stable isotopes in Morchella with values from concurrently collected foliage, the ectomycorrhizal Geopyxis carbonaria (Alb. & Schwein.) Sacc., the saprotrophic Plicaria endocarpoides (Berk.) Rifai, and with literature to determine isotopic values for ectomycorrhizal or saprotrophic fungi. Geopyxis, Plicaria and Morchella, respectively, were 3 parts per thousand, 5 parts per thousand and 6 parts per thousand higher in C-13 than foliage and 5 parts per thousand, 7 parts per thousand and 7 parts per thousand higher in N-15. High N-15 enrichment in Morchella indicated that recent litter was not the primary source for Morchella nitrogen, and similar C-13 and N-15 enrichments to Plicaria suggest that Morchella assimilates its carbon and nitrogen from the same source pool as this saprotrophic fungus. From radiocarbon analyses Morchella averaged 11 +/- 6 y old (n = 19), Plicaria averaged 17 +/- 5 y old (n = 3), foliage averaged 1 +/- 2 y old (n = 8) and Geopyxis (n = 1) resembled foliage in Delta C-14. We conclude that morels fruiting in post-fire environments in our study assimilated old carbon and were saprotrophic. C1 [Hobbie, Erik A.; Rice, Samuel F.] Univ New Hampshire, Earth Syst Res Ctr, Durham, NH 03824 USA. [Weber, Nancy S.] Oregon State Univ, Dept Forest Ecosyst & Soc, 2160 NW Beechwood Pl, Corvallis, OR 97330 USA. [Smith, Jane E.] Forest Serv, USDA, Pacific Northwest Res Stn, Forestry Sci Lab, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. RP Hobbie, EA (reprint author), Univ New Hampshire, Earth Syst Res Ctr, Durham, NH 03824 USA. EM Erik.Hobbie@unh.edu FU USDA Forest Service, Pacific Northwest Research Station; U.S. National Science Foundation [DEB-1146328] FX Financial support for this research was provided by the USDA Forest Service, Pacific Northwest Research Station, and by the U.S. National Science Foundation, grant DEB-1146328. Mention of trade or firm names does not constitute an endorsement by the US Department of. Agriculture. We thank Knut Kielland for boat transportation to the Tanana River site in Alaska and thank Steve Macko for stable isotope analyses. Tricia L. Wurtz contributed collections and initial ideas for the study. Tom Horton, Ari Jumpponen, Amy Rossman and Steve Trudell provided comments on an earlier version of the manuscript, and Ernst Linder and Greg Brenner provided statistical advice. NR 41 TC 0 Z9 0 U1 4 U2 4 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD JUL-AUG PY 2016 VL 108 IS 4 BP 638 EP 645 DI 10.3852/15-281 PG 8 WC Mycology SC Mycology GA DV8WK UT WOS:000383217200002 PM 27153881 ER PT J AU Campbell, AS Ploetz, RC Dreaden, TJ Kendra, PE Montgomery, WS AF Campbell, Alina S. Ploetz, Randy C. Dreaden, Tyler J. Kendra, Paul E. Montgomery, Wayne S. TI Geographic variation in mycangial communities of Xyleborus glabratus SO MYCOLOGIA LA English DT Article DE ambrosia beetle; avocado; laurel wilt; mycangia; Raffaelea lauricola; symbiont; Xyleborus glabratus ID COLEOPTERA-CURCULIONIDAE SCOLYTINAE; REDBAY AMBROSIA BEETLE; RAFFAELEA-LAURICOLA; FUNGI; SYMBIONT; DNA; ABUNDANCE; PATHOGEN; DYNAMICS; HISTORY AB Factors that influence fungal communities in ambrosia beetle mycangia are poorly understood. The beetle that is responsible for spreading laurel wilt in SE USA, Xyleborus glabratrus, was examined at three sites along a 500 km N-S transect in Florida, each populated by host trees in the Lauraceae. Fungal phenotypes were quantified in mycangia of individual females that were collected from a site in Miami-Dade County (MDC), 25.8N, with swamp bay (Persea palustris), one in Highlands County (HC), 27.9N, with silk bay (P. humulis) and swamp bay and another in Alachua County (AC), 29.8N, with redbay (P. borbonia). Based on combined LSU, SSU and beta-tubulin datasets the most prominent phenotypes were Raffaelea lauricola (cause of laurel wilt), R. subalba, R. subfusca, R. fusca, R. arxii and an undescribed Raffaelea sp. Mean numbers of colony forming units (CFUs) of R. lauricola varied by location (P < 0.003), and a multivariate analysis, which accounted for the presence and relative abundance of fungal species, indicated that there were significant variations in mycangial communities among the sites; thus climate and vegetation might have affected fungal diversity and the relative abundance of these fungi in the mycangia of X glabratus. Statistically it was unlikely that any of the species influenced the presence and prevalence of another species. C1 [Campbell, Alina S.; Ploetz, Randy C.] Univ Florida, Trop Res & Educ Ctr, Homestead, FL 33031 USA. [Dreaden, Tyler J.] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [Dreaden, Tyler J.] Forest Serv, Southern Res Stn, USDA, Forest Hlth Res & Educ Ctr, Lexington, KY 40517 USA. [Kendra, Paul E.; Montgomery, Wayne S.] USDA ARS, Subtrop Hort Res Stn, Miami, FL 33158 USA. RP Campbell, AS (reprint author), Univ Florida, Trop Res & Educ Ctr, Homestead, FL 33031 USA. EM alinascampbell@ufl.edu FU USDA NIFA grant [2009-51181-05915] FX This research was financed partially by USDA NIFA grant 2009-51181-05915. We thank Dr James Colee (UF-IFAS-Statistics Department) for help with the statistical analysis. Thanks also to Josh Konkol, Jill Ploetz and Ronald Wideman for their help with finding and processing the beetles. NR 35 TC 3 Z9 3 U1 7 U2 7 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD JUL-AUG PY 2016 VL 108 IS 4 BP 657 EP 667 DI 10.3852/15-133 PG 11 WC Mycology SC Mycology GA DV8WK UT WOS:000383217200004 PM 27055571 ER PT J AU Zhou, X O'Donnell, K Aoki, T Smith, JA Kasson, MT Cao, ZM AF Zhou, Xue O'Donnell, Kerry Aoki, Takayuki Smith, Jason A. Kasson, Matthew T. Cao, Zhi-Min TI Two novel Fusarium species that cause canker disease of prickly ash (Zanthoxylum bungeanum) in northern China form a novel Glade with Fusarium torreyae SO MYCOLOGIA LA English DT Article DE Fusarium continuum; F. zanthoxyli; genealogical concordance; molecular phylogenetics; morphology; RPB2; TEF1 ID SUDDEN-DEATH SYNDROME; COMPLEX; SYSTEMATICS; TUCUMANIAE; FUNGI; RPB2 AB Canker disease of prickly ash (Zanthoxylum bungeanum) has caused a decline in the production of this economically important spice in northern China in the past 25 y. To identify the etiological agent, 38 fungal isolates were recovered from symptomatic tissues from trees in five provinces in China. These isolates were identified by conducting BLASTN queries of NCBI GenBank and phylogenetic analyses of DNA sequence data from the nuclear ribosomal internal transcribed spacer region (ITS rDNA), a portion of the translation elongation factor 1-alpha (TEF1) gene, and genes encoding RNA polymerase II largest (RPB1) and second largest (RPB2) subunits. Results of these analyses suggested that 30/38 isolates belonged to two novel fusaria most closely related to the Florida torreya (Torreya taxifolia Arm.) pathogen, Fusarium torreyae in Florida and Georgia. These three canker-inducing tree pathogens form a novel Glade within Fusarium here designated the F torreyae species complex (FTOSC). BLASTN queries of GenBank also revealed that 5/38 isolates recovered from cankers represented an undescribed phylogenetic species within the F. solani species complex (FSSC) designated FSSC 6. Stem inoculations of three fusaria on Z bungeanum resulted in consistent canker symptomg from which these three fusaria were recovered. The two novel fusaria, however, induced significantly larger lesions than FSSC 6. Herein, the two novel prickly ash pathogeris are formally described as E zanthoxyli and F continuum. C1 [Zhou, Xue; Cao, Zhi-Min] Northwest A&F Univ, Coll Forestry, Taicheng Rd, Yangling 712100, Shaanxi, Peoples R China. [O'Donnell, Kerry] ARS, USDA, Mycotoxin Prevent & Appl Microbiol Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 60604 USA. [Aoki, Takayuki] Natl Agr & Food Res Org, Genet Resources Ctr MAFF, 2-1-2 Kannondai, Tsukuba, Ibaraki 3058602, Japan. [Smith, Jason A.] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [Kasson, Matthew T.] West Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA. RP Cao, ZM (reprint author), Northwest A&F Univ, Coll Forestry, Taicheng Rd, Yangling 712100, Shaanxi, Peoples R China. EM zmcao@nwsuaf.edu.cn FU National Science Technology Support Project [2012BAD19B08] FX This research was supported in part by the National Science Technology Support Project (No. 2012BAD19B08). The mention of company or trade products does not imply that they are endorsed or recommended by the US Department of Agriculture over other companies or similar products not mentioned. The USDA is an equal opportunity provider and employer. We thank Stacy Sink for assistance with the DNA sequence data and Ning Xie and Jiefei Wang for help collecting specimens. NR 38 TC 2 Z9 2 U1 6 U2 6 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD JUL-AUG PY 2016 VL 108 IS 4 BP 668 EP 681 DI 10.3852/15-189 PG 14 WC Mycology SC Mycology GA DV8WK UT WOS:000383217200005 PM 27055569 ER PT J AU Zhang, J Yang, H Yu, QY Wu, MD Yang, L Zhuang, WY Chen, WD Li, GQ AF Zhang, J. Yang, H. Yu, Q. Y. Wu, M. D. Yang, L. Zhuang, W. Y. Chen, W. D. Li, G. Q. TI Botrytis pyriformis sp nov., a novel and likely saprophytic species of Botrytis SO MYCOLOGIA LA English DT Article DE Botrytis pyriformis; molecular identification; saprophyte; Sedum sarmentosum ID MOLD DISEASE; GRAY MOLD; CINEREA; PATHOGEN; PCR; IDENTIFICATION; HYPOVIRULENCE; PHYLOGENY; VIRULENCE; ELLIPTICA AB A novel species of Botrytis from Sedum sarmentosum was described based on morphology and analyses of DNA sequences of nuc rDNA ITS regions and three nuclear genes (G3PDH, HSP60, RPB2). Meanwhile pathogenicity in 32 plant species, response to temperature for growth and conidial germination for the species were determined. The Botrytis species was named Botrytis pyriformis sp. nov. It was characterized by formation of grayish mycelia, brownish conidia and melanized sclerotia on PDA. The conidia are pear-shaped, melanized and covered with abundant villiform appendages on the conidial surface. Comparison of the ITS sequences confirmed its placement in the genus Botrytis. Phylogenetic analysis based on DNA sequences of G3PDH, HSP60 and RPB2 genes indicated that B. pyriformis and other 30 Botrytis species form a monophyletic Glade, which was further divided into three subclades. Subclade I comprised B. pyriformis alone, whereas subclades II and III comprised six and 24 Botrytis species, respectively. Botrytis pyriformis could not infect 32 plant species including S. sarmentosum, possibly due to deficiency in formation of infection cushions. This study presents a formal description and illustrations for B. pyriformis and provides experimental evidence, indicating that B. pyriformis might be a saprophytic species. C1 [Zhang, J.; Yang, H.; Yu, Q. Y.; Wu, M. D.; Yang, L.; Li, G. Q.] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China. [Zhang, J.; Yang, H.; Yu, Q. Y.; Wu, M. D.; Yang, L.; Li, G. Q.] Huazhong Agr Univ, Key Lab Plant Pathol Hubei Prov, Wuhan 430070, Peoples R China. [Zhuang, W. Y.] Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing 100101, Peoples R China. [Chen, W. D.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. RP Li, GQ (reprint author), Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China.; Li, GQ (reprint author), Huazhong Agr Univ, Key Lab Plant Pathol Hubei Prov, Wuhan 430070, Peoples R China. EM guoqingli@mail.hzau.edu.cn FU National Natural Science Foundation of China [31301615]; Special Fund for Agro-scientific Research in the Public Interest [201303025]; Applied Basic Research Program of Wuhan Science and Technology Bureau [2014020101010072] FX This study was financially supported by the National Natural Science Foundation of China (Grant No. 31301615), the Special Fund for Agro-scientific Research in the Public Interest (Grant No. 201303025) and Applied Basic Research Program of Wuhan Science and Technology Bureau (Grant No. 2014020101010072). NR 45 TC 1 Z9 1 U1 5 U2 5 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD JUL-AUG PY 2016 VL 108 IS 4 BP 682 EP 696 DI 10.3852/15-340 PG 15 WC Mycology SC Mycology GA DV8WK UT WOS:000383217200006 PM 27153884 ER PT J AU Schroers, HJ Samuels, GJ Zhang, N Short, DPG Juba, J Geiser, DM AF Schroers, Hans-Josef Samuels, Gary J. Zhang, Ning Short, Dylan P. G. Juba, Jean Geiser, David M. TI Epitypification of Fusisporium (Fusarium) solani and its assignment to a common phylogenetic species in the Fusarium solani species complex SO MYCOLOGIA LA English DT Article DE FSSC; Hypocreales; Nectriaceae; potato dry rot; taxonomy; translation elongation factor 1-alpha gene; von Martius ID DRY ROT; CONTACT-LENS; POTATO; INFECTIONS; PATHOGEN; IDENTIFICATION; POPULATIONS; DIVERSITY; MEMBERS; ORIGIN AB Fusisporium solani was described as the causal agent of a dry rot of potato in Germany in the mid 19th century. As Fusarium solani, the species became known as a plurivorous plant pathogen, endophyte, decomposer, and opportunistic pathogen of humans and nutritional symbiont of insects. In parallel, it became evident that the morphologically defined species F. solani represents a phylogenetically and biologically complex group of often morphologically cryptic species that has come to be known in part as the F. solani species complex (FSSC), accommodating several formae speciales and mating populations/biological species. The FSSC currently includes more than 60 phylogenetic species. Several of these have been named, but the majority remains unnamed and the identity of F. solani sensu stricto is unclear. To promote further taxonomic developments in the FSSC, lecto- and epitypification is proposed for Fusisporium solani. Although no type material for F. solani is known to exist, the species was abundantly illustrated in the protologue. Thus, a relevant illustration provided by von Martius is selected as the lectotype. The epitype selected here originates from a rotting potato collected in a field in Slovenia. This strain causes a dry rot of artificially inoculated potatoes. It groups in the heretofore unnamed phylogenetic species 5, which is nested within Glade 3 of the FSSC (FSSC 5). Members Of this phylogenetic species have a wide geographic distribution and include soil saprotrophs and plant and opportunistic human pathogens. This typification is consistent with the original description of Fusisporium solani and the concept of F. solani, as a widely distributed soil inhabitant and pathogen. C1 [Schroers, Hans-Josef] Agr Inst Slovenia, Ljubljana 1000, Slovenia. [Samuels, Gary J.] ARS, USDA, Systemat Mycol & Microbiol Lab, B-010A,10300 Baltimore Ave, Beltsville, MD 20705 USA. [Zhang, Ning] Rutgers State Univ, Dept Plant Biol & Pathol, Cook Campus, New Brunswick, NJ 08901 USA. [Zhang, Ning] Rutgers State Univ, Dept Biochem & Microbiol, Cook Campus, New Brunswick, NJ 08901 USA. [Short, Dylan P. G.] Univ Calif Davis, Salinas Res Stn, Dept Plant Pathol, Salinas, CA 93905 USA. [Juba, Jean; Geiser, David M.] Penn State Univ, Fusarium Res Ctr, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. [Samuels, Gary J.] 321 Hedgehog Mt Rd, Deering, NH 03244 USA. RP Schroers, HJ (reprint author), Agr Inst Slovenia, Ljubljana 1000, Slovenia. EM hans.schroers@kis.si RI Zhang, Ning/K-3046-2012 OI Zhang, Ning/0000-0003-0755-2505 FU National Science Foundation, NSF [07-31510, DEB 0089474]; National Institute of Food and Agriculture at the Pennsylvania State Agricultural Experiment Station [PEN 04527]; NIFA, Education in Genomics-Based Microbial Forensics [2010-65110-20488]; Slovenian Research Agency (ARKS) [P4-0072] FX We gratefully acknowledge the assistance of the curators and staff of the herbaria BR (National Botanic Garden of Belgium, Meise), G (Conservatoire et Jardin botaniques de la Ville de Geneve, Switzerland), M (Botanische Staatssammlung Munchen, Germany), MEL (Royal Botanic Gardens, Australia, Victoria, Melbourne), TO (University of Turin, Italy) and W (Naturhistorisches Museum Wien, Austria). We thank Mrs. M. Kralj Kuncic for collecting potatoes from Begunje and Stacy Sink at the United States Department of Agriculture National Center for Agricultural Utilization Research Mycotoxin Prevention and Applied Microbiology Research Unit for the species and haplotype assignments. This work was supported by grants from the National Science Foundation, NSF PEET grant 07-31510 to Priscila Chaverri, Amy Rossman and GJS, and NSF DEB 0089474 to DMG and GJS. This work was supported in part by the National Institute of Food and Agriculture Project PEN 04527 at the Pennsylvania State Agricultural Experiment Station. DPGS was supported by NIFA grant 2010-65110-20488, Education in Genomics-Based Microbial Forensics. The Slovenian Research Agency (ARKS) supported the study through financing the research program Agrobiodiversity P4-0072. NR 51 TC 3 Z9 3 U1 3 U2 3 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD JUL-AUG PY 2016 VL 108 IS 4 BP 806 EP 819 DI 10.3852/15-255 PG 14 WC Mycology SC Mycology GA DV8WK UT WOS:000383217200017 PM 27055573 ER PT J AU Chen, SH Yu, J Li, QW Zhao, JP Wedge, DE Duke, SO Liao, DF Wang, YH Fronczek, FR Khan, IA Wang, W AF Chen, Sheng-Huang Yu, Juan Li, Qin-Wen Zhao, Jian-Ping Wedge, David E. Duke, Stephen O. Liao, Duan-Fang Wang, Yu-Hong Fronczek, Frank R. Khan, Ikhlas A. Wang, Wei TI 7 alpha-Hydroxyfriedelan-3-one-26-o1-29-oic acid and other Constituents from Pileostegia viburnoides var. glabrescens SO NATURAL PRODUCT COMMUNICATIONS LA English DT Article DE Pileostegia viburnoides var. glabrescens; Chemical constituents; Anti-inflammatory activity; Cytotoxicity; Phytotoxicity ID CHEMICAL-CONSTITUENTS; TRITERPENES; GLYCOSIDES; PHYTOCHEMICALS; COUMARINS; EXTRACT; GROWTH; LIGNAN; ROOTS; BARK AB Phytochemical investigation of the roots and rhizomes of Pileostegia viburnoides var. glabrescens led to the isolation and identification of 31 compounds (1-31), 25 of which (1-2, 4-7, 9-12, 15-18, 21-31) were isolated from the Pileostegia genus for the first time. 7 alpha-Hydroxyfriedelan-3-one-26-o1-29-oic acid (1) is a new friedelane-type triterpene. The structure of n-butyl-13-D-fructopyranoside (2) was determined by single-crystal X-ray diffraction. Compounds 4 and 12 displayed marginal cytotoxicity against the P388 murine leukemia cell line with IC50 values of 13.4 mu M and 25.0 mu M, respectively. Compound 23 exhibited marginal anti-inflammatory activity by the inhibition of lipopolysaccharide induced nitric oxide production in RAW 264.7 macrophage cells, with an IC50 value of 32.0 mu M. Compounds 3, 8-10, 25, and 27 were phytotoxic to the dicot Lactuca sativa (lettuce) and/or the monocot Agrostis stolonifera (bentgrass). C1 [Chen, Sheng-Huang; Yu, Juan; Li, Qin-Wen; Liao, Duan-Fang; Khan, Ikhlas A.; Wang, Wei] Hunan Univ Chinese Med, Sch Pharm, Sino Luxemburg TCM Res Ctr, TCM & Ethnomed Innovat & Dev Lab, Changsha 410208, Hunan, Peoples R China. [Zhao, Jian-Ping; Khan, Ikhlas A.] Univ Mississippi, Sch Pharm, Pharmaceut Sci Res Inst, Natl Ctr Nat Prod Res, University, MS 38677 USA. [Wedge, David E.; Duke, Stephen O.] Univ Mississippi, ARS, USDA, Nat Prod Utilizat Res Unit,Natl Ctr Nat Prod Res, University, MS 38677 USA. [Wang, Yu-Hong] Hunan Univ Chinese Med, State Key Lab Chinese Med Powder & Med Innovat Hu, Changsha 410208, Hunan, Peoples R China. [Fronczek, Frank R.] Louisiana State Univ, Dept Chem, Baton Rouge, LA 70803 USA. RP Wang, W (reprint author), Hunan Univ Chinese Med, Sch Pharm, Sino Luxemburg TCM Res Ctr, TCM & Ethnomed Innovat & Dev Lab, Changsha 410208, Hunan, Peoples R China. EM wangwei402@hotmail.com FU Changsha Science and Technology Bureau Project [80010369, K1205019-31]; Hunan Province University Innovation Platform Open Fund Project [12K086, 13K077]; Himan Key Laboratory of Chinese Materia Medica Powder and Innovative Drugs Established by Provincial and Ministry [ZYFT201402]; Division of Stem Cell University of Chinese Medicine; Chinese Materia Medica, Hunan University of Chinese Medicine FX This work was financially supported by the Changsha Science and Technology Bureau Project (80010369 and K1205019-31), Hunan Province University Innovation Platform Open Fund Project (12K086 and 13K077), Himan Key Laboratory of Chinese Materia Medica Powder and Innovative Drugs Established by Provincial and Ministry (ZYFT201402). We thank Dr Shabana I. Khan from the University of Mississippi and Mr Robert Johnson from USDA for their technical assistance with the anti-inflammatory activity assay and herbicide bioassays, respectively. We are grateful to the Division of Stem Cell University of Chinese Medicine for their partial financial support. Regulation and Application with Chinese Materia Medica, Hunan University of Chinese Medicine for their partial financial support. NR 40 TC 0 Z9 0 U1 1 U2 1 PU NATURAL PRODUCTS INC PI WESTERVILLE PA 7963 ANDERSON PARK LN, WESTERVILLE, OH 43081 USA SN 1934-578X EI 1555-9475 J9 NAT PROD COMMUN JI Nat. Prod. Commun. PD JUL PY 2016 VL 11 IS 7 BP 931 EP 934 PG 4 WC Chemistry, Medicinal; Food Science & Technology SC Pharmacology & Pharmacy; Food Science & Technology GA DS8XI UT WOS:000381065900016 ER PT J AU Costello, RB Dwyer, JT Bailey, RL AF Costello, Rebecca B. Dwyer, Johanna T. Bailey, Regan L. TI Chromium supplements for glycemic control in type 2 diabetes: limited evidence of effectiveness SO NUTRITION REVIEWS LA English DT Review DE chromium; fasting plasma glucose; hemoglobin A1c; type 2 diabetes mellitus ID PLACEBO-CONTROLLED TRIAL; TOTAL PARENTERAL-NUTRITION; GLUCOSE-TOLERANCE; DOUBLE-BLIND; INSULIN-RESISTANCE; BREWERS-YEAST; SERUM-LIPIDS; INORGANIC CHROMIUM; DIETARY CHROMIUM; BODY-COMPOSITION AB Some adults with type 2 diabetes mellitus (T2DM) believe that chromium-containing supplements will help control their disease, but the evidence is mixed. This narrative review examines the efficacy of chromium supplements for improving glycemic control as measured by decreases in fasting plasma glucose (FPG) or hemoglobin A1c (HbA1c). Using systematic search criteria, 20 randomized controlled trials of chromium supplementation in T2DM patients were identified. Clinically meaningful treatment goals were defined as an FPG of <= 7.2mmol/dL, a decline in HbA1c to <= 7%, or a decrease of >= 0.5% in HbA1c. In only a few randomized controlled trials did FPG (5 of 20), HbA1c (3 of 14), or both (1 of 14) reach the treatment goals with chromium supplementation. HbA1c declined by >= 0.5% in 5 of 14 studies. On the basis of the low strength of existing evidence, chromium supplements have limited effectiveness, and there is little rationale to recommend their use for glycemic control in patients with existing T2DM. Future meta-analyses should include only high-quality studies with similar forms of chromium and comparable inclusion/exclusion criteria to provide scientifically sound recommendations for clinicians. C1 [Costello, Rebecca B.; Dwyer, Johanna T.; Bailey, Regan L.] NIH, Off Dietary Supplements, 6100 Execut Blvd,Ste 3B01, Bethesda, MD 20892 USA. [Dwyer, Johanna T.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Sch Med, Boston, MA 02111 USA. [Dwyer, Johanna T.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Bailey, Regan L.] Purdue Univ, Dept Nutr Sci, W Lafayette, IN 47907 USA. RP Costello, RB (reprint author), NIH, Off Dietary Supplements, 6100 Execut Blvd,Ste 3B01, Bethesda, MD 20892 USA. EM costellb@od.nih.gov FU Office of Dietary Supplements of the National Institutes of Health, US Department of Health and Human Services; US Department of Agriculture, Agricultural Research Service [58-1950-0-014] FX This work was supported by the Office of Dietary Supplements of the National Institutes of Health, US Department of Health and Human Services. Partial support was also provided by the US Department of Agriculture, Agricultural Research Service, under agreement no. 58-1950-0-014. NR 75 TC 0 Z9 0 U1 10 U2 10 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0029-6643 EI 1753-4887 J9 NUTR REV JI Nutr. Rev. PD JUL PY 2016 VL 74 IS 7 BP 455 EP 468 DI 10.1093/nutrit/nuw011 PG 14 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DV9RO UT WOS:000383279100004 PM 27261273 ER PT J AU Youlton, C Wendland, E Anache, JAA Poblete-Echeverr-A, C Dabney, S AF Youlton, Cristian Wendland, Edson Ayach Anache, Jamil Alexandre Poblete-Echeverr-A, Carlos Dabney, Seth TI Changes in Erosion and Runoff due to Replacement of Pasture Land with Sugarcane Crops SO SUSTAINABILITY LA English DT Article DE sediment; land use; experimental plots; Brazil ID BIO-ETHANOL PRODUCTION; SOIL-EROSION; NUTRIENTS LOSSES; WATER LOSSES; PLOT SCALE; SAO-PAULO; BRAZIL; COVER; DEGRADATION; CHALLENGES AB The planting of sugarcane crops has expanded in the last decade in the southeast of Brazil, mainly due to its use for biofuel production, such as ethanol. This expansion in the State of Sao Paulo has occupied land that was previously used for cattle production. The change in land use affects soil and water through changes in ground cover and disturbance associated with farming practices. The objective of the following study was to determine the impact on runoff and erosion resulting from the conversion of pastureland to sugarcane for biofuel production. Erosion plots measuring 100 m(2) were built on a farm in Itirapina-SP, Brazil, on land with a slope gradient of 9% and soil composed of Quartz-sand Neosols (Typic quartzipsaments). The treatments were an 18-year old pasture and a new sugarcane plantation, with three replicates for each. After each rainfall episode, erosion and runoff were monitored during the first and second years after sugarcane was planted. The results show increased runoff and soil loss during the first year, though levels decreased in the second year when the sugarcane residue mulch ground cover increased. In addition, the necessary rainfall characteristics (e.g., intensity, duration) required to produce runoff and soil erosion were identified. C1 [Youlton, Cristian; Poblete-Echeverr-A, Carlos] Pontificia Univ Catolica Valparaiso, Escuela Agron, San Francisco S-N, Quillota 2340025, Chile. [Youlton, Cristian; Wendland, Edson; Ayach Anache, Jamil Alexandre] Univ Sao Paulo, Escola Engn Sao Carlos, BR-13566590 Sao Carlos, SP, Brazil. [Dabney, Seth] USDA ARS, Watershed Phys Proc Res Unit, Natl Sedimentat Lab, Box 1157, Oxford, MS 38655 USA. RP Youlton, C (reprint author), Pontificia Univ Catolica Valparaiso, Escuela Agron, San Francisco S-N, Quillota 2340025, Chile.; Youlton, C (reprint author), Univ Sao Paulo, Escola Engn Sao Carlos, BR-13566590 Sao Carlos, SP, Brazil. EM cristian.youlton@pucv.cl; ew@sc.usp.br; jamil.anache@usp.br; carlos.poblete@pucv.cl; seth.dabney@ars.usda.gov RI Cepid, CeMEAI/J-2417-2015; Anache, Jamil/A-5296-2017; OI Anache, Jamil/0000-0002-4460-2914; Youlton, Cristian/0000-0002-2369-6990; Poblete-Echeverria, Carlos/0000-0001-8025-5879; Wendland, Edson/0000-0003-3374-608X FU Foundation for Research Support of the State of Sao Paulo (Fundacao de Amparoa Pesquisa do Estado de Sao Paulo-FAPESP) [2010/00251-5]; National Council for Scientific and Technological Development (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPq) FX The authors would like to thank the Foundation for Research Support of the State of Sao Paulo (Fundacao de Amparoa Pesquisa do Estado de Sao Paulo-FAPESP) for funding the present study (Processo 2010/00251-5), the National Council for Scientific and Technological Development (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPq) for the first author's doctoral fellowship and the Arruda Botelho Institute for facilitating the study area and installations at the San Jose farm in Itirapina, SP. NR 47 TC 0 Z9 0 U1 9 U2 9 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2071-1050 J9 SUSTAINABILITY-BASEL JI Sustainability PD JUL PY 2016 VL 8 IS 7 AR 685 DI 10.3390/su8070685 PG 12 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Environmental Sciences; Environmental Studies SC Science & Technology - Other Topics; Environmental Sciences & Ecology GA DS4OM UT WOS:000380760400093 ER PT J AU Premke, K Attermeyer, K Augustin, J Cabezas, A Casper, P Deumlich, D Gelbrecht, J Gerke, HH Gessler, A Grossart, HP Hilt, S Hupfer, M Kalettka, T Kayler, Z Lischeid, G Sommer, M Zak, D AF Premke, Katrin Attermeyer, Katrin Augustin, Juergen Cabezas, Alvaro Casper, Peter Deumlich, Detlef Gelbrecht, Joerg Gerke, Horst H. Gessler, Arthur Grossart, Hans-Peter Hilt, Sabine Hupfer, Michael Kalettka, Thomas Kayler, Zachary Lischeid, Gunnar Sommer, Michael Zak, Dominik TI The importance of landscape diversity for carbon fluxes at the landscape level: small-scale heterogeneity matters SO WILEY INTERDISCIPLINARY REVIEWS-WATER LA English DT Article ID DISSOLVED ORGANIC-CARBON; GREENHOUSE-GAS BALANCE; CLIMATE-CHANGE MITIGATION; NITROUS-OXIDE EMISSION; TEMPERATE FOREST SOIL; TERRESTRIAL CARBON; METHANE EMISSIONS; BOREAL LAKES; KETTLE HOLES; HYDROELECTRIC RESERVOIRS AB Landscapes can be viewed as spatially heterogeneous areas encompassing terrestrial and aquatic domains. To date, most landscape carbon (C) fluxes have been estimated by accounting for terrestrial ecosystems, while aquatic ecosystems have been largely neglected. However, a robust assessment of C fluxes on the landscape scale requires the estimation of fluxes within and between both landscape components. Here, we compiled data from the literature on C fluxes across the air-water interface from various landscape components. We simulated C emissions and uptake for five different scenarios which represent a gradient of increasing spatial heterogeneity within a temperate young moraine landscape: (I) a homogeneous landscape with only cropland and large lakes; (II) separation of the terrestrial domain into cropland and forest; (III) further separation into cropland, forest, and grassland; (IV) additional division of the aquatic area into large lakes and peatlands; and (V) further separation of the aquatic area into large lakes, peatlands, running waters, and small water bodies These simulations suggest that C fluxes at the landscape scale might depend on spatial heterogeneity and landscape diversity, among other factors. When we consider spatial heterogeneity and diversity alone, small inland waters appear to play a pivotal and previously underestimated role in landscape greenhouse gas emissions that may be regarded as C hot spots. Approaches focusing on the landscape scale will also enable improved projections of ecosystems' responses to perturbations, e.g., due to global change and anthropogenic activities, and evaluations of the specific role individual landscape components play in regional C fluxes. (C) 2016 The Authors. WIREs Water published by Wiley Periodicals, Inc. C1 [Premke, Katrin; Attermeyer, Katrin; Cabezas, Alvaro; Gelbrecht, Joerg; Hilt, Sabine; Hupfer, Michael; Zak, Dominik] Leibniz Inst Freshwater Ecol & Inland Fisheries I, Chem Analyt & Biogeochem, Berlin, Germany. [Premke, Katrin; Augustin, Juergen; Gessler, Arthur; Kayler, Zachary] Leibniz Ctr Agr Landscape Res ZALF, Inst Landscape Biogeochem, Muncheberg, Germany. [Casper, Peter; Grossart, Hans-Peter] Leibniz Inst Freshwater Ecol & Inland Fisheries I, Expt Limnol, Berlin, Germany. [Deumlich, Detlef; Gerke, Horst H.; Sommer, Michael] Leibniz Ctr Agr Landscape Res ZALF, Inst Soil Landscape, Muncheberg, Germany. [Gessler, Arthur] Swiss Fed Inst Forest Snow & Landscape Res WSL, Birmensdorf, Switzerland. [Grossart, Hans-Peter] Univ Potsdam, Inst Biochem & Biol, Potsdam, Germany. [Kalettka, Thomas; Lischeid, Gunnar] Leibniz Ctr Agr Landscape Res ZALF, Inst Landscape Hydrol, Muncheberg, Germany. [Kayler, Zachary] US Forest Serv, USDA, Northern Res Stn, Lawrence Livermore Natl Lab, Livermore, CA 94550 USA. [Lischeid, Gunnar; Sommer, Michael] Univ Potsdam, Inst Earth & Environm Sci, Potsdam, Germany. [Zak, Dominik] Aarhus Univ, Dept Biosci, Aarhus, Denmark. RP Premke, K (reprint author), Leibniz Inst Freshwater Ecol & Inland Fisheries I, Chem Analyt & Biogeochem, Berlin, Germany.; Premke, K (reprint author), Leibniz Ctr Agr Landscape Res ZALF, Inst Landscape Biogeochem, Muncheberg, Germany. EM premke@igb-berlin.de RI Gessler, Arthur/C-7121-2008 OI Gessler, Arthur/0000-0002-1910-9589 NR 156 TC 1 Z9 1 U1 13 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA EI 2049-1948 J9 WIRES-WATER JI Wiley Interdiscip. Rev.-Water PD JUL-AUG PY 2016 VL 3 IS 4 BP 601 EP 617 DI 10.1002/wat2.1147 PG 17 WC Water Resources SC Water Resources GA DV4DH UT WOS:000382874800009 ER PT J AU Gisbert, C Dumm, JM Prohens, J Vilanova, S Stommel, JR AF Gisbert, Carmina Dumm, Judith M. Prohens, Jaime Vilanova, Santiago Stommel, John R. TI A Spontaneous Eggplant (Solanum melongena L.) Color Mutant Conditions Anthocyanin-free Fruit Pigmentation SO HORTSCIENCE LA English DT Article DE gene regulation; Myb; Myc; Solanum melongena; structural gene; regulatory gene; fruit quality ID TRANSCRIPTION FACTORS; BIOSYNTHESIS; GENES; ACCUMULATION; EVOLUTION; GENETICS; ARABIDOPSIS; REVEALS; PATHWAY; TOMATO AB We identified a single plant in a grow out of the eggplant (Solanum melongena L.) variety 'Black Beauty' bearing green fruit. 'Black Beauty' normally produces violet/black pigmented fruit attributed to anthocyanin accumulation. We selected the green-fruited true-breeding genotype E13GB42 from the S2 generation obtained from selfing of the SO green-fruited color mutant. Characterization of 12 simple sequence repeat (SSR) markers, eight fruit morphological attributes and fruit yield support E13GB42 arising as a spontaneous mutant of 'Black Beauty'. With the exception of fruit calyx prickliness, E13GB42 was not significantly different from 'Black Beauty' for fruit morphological attributes and yield. E13GB42 exhibited an SSR marker profile identical to that of 'Black Beauty' but polymorphic with that of eight violet/black-fruited modern eggplant hybrids, older open-pollinated varieties and landraces. Transcript levels of key anthocyanin biosynthetic genes (Chs, Dfr, and Ans) and regulatory genes (Myb(c), Myc, and Wd) were significantly lower in the green-fruited E13GB42 mutant in comparison with the black fruited variety 'Black Beauty' at various stages of fruit development ranging from small post-anthesis fruit to full-size marketable fruit. Progeny obtained from selfing of the original mutant and reciprocal crosses with 'Black Beauty' produced violet, green, and green with violet striped color classes that together were not compatible with one or two gene inheritance models, suggesting that the mutation responsible for the E13GB42 phenotype influences multiple genetic factors that control fruit pigmentation. C1 [Gisbert, Carmina; Prohens, Jaime; Vilanova, Santiago] Univ Politecn Valencia, Inst Conservac & Mejora Agrodiversidad Valenciana, Camino de Vera 14, E-46022 Valencia, Spain. [Dumm, Judith M.; Stommel, John R.] ARS, Genet Improvement Fruits & Vegetables Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Stommel, JR (reprint author), ARS, Genet Improvement Fruits & Vegetables Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. EM john.stommel@ars.usda.gov FU Ministerio de Economia y Competitividad; FEDER [AGL2012-34213] FX Activities performed by the Spanish team have been funded by Ministerio de Economia y Competitividad and FEDER (grant AGL2012-34213). Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. NR 36 TC 1 Z9 1 U1 10 U2 10 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD JUL PY 2016 VL 51 IS 7 BP 793 EP 798 PG 6 WC Horticulture SC Agriculture GA DT1KJ UT WOS:000381240900001 ER PT J AU Stover, E Hall, DG Shatters, RG Moore, GA AF Stover, Ed Hall, David G. Shatters, Robert G., Jr. Moore, Gloria A. TI Influence of Citrus Source and Test Genotypes on Inoculations with Candidatus Liberibacter asiaticus SO HORTSCIENCE LA English DT Article DE Asian citrus psyllid; citrus greening; Diaphorina citri; disease resistance; disease tolerance; HLB; huanglongbing ID HUANGLONGBING DISEASE; INFECTION; TOLERANCE AB Assessments of the resistance of citrus germplasm to huanglongbing (HLB) can be expedited by inoculating plants under laboratory or greenhouse settings with the HLB bacterium, Candidatus Liberibacter asiaticus (CLas). Consistent rapid screening is critical to efficiently assess disease resistance among plant materials; however, a number of factors may govern the efficacy of such inoculations. Despite the rapidity at which HLB can spread in a grove, it often takes 8 to 10 months for high levels of CLas and HLB symptoms to develop even in highly susceptible sweet orange. Therefore, two experiments were conducted to assess factors that might influence efficiency in screening for HLB resistance. In one experiment, three test citrus genotypes ('Kuharske', previously shown to be HLB resistant; rough lemon, previously shown to be HLB tolerant; and 'Valencia', HLB susceptible) were bud grafted using CLas-infected buds from four different source genotypes. All bud source genotypes had similar levels of CLas titer, but citron, rough lemon, and Volkamer lemon were hypothesized to be better bud inoculum sources as they are more tolerant of HLB than 'Valencia'. Among the three test genotypes over all sources of infected buds, inoculations of 'Kuharske' resulted in lower CLas titers and fewer HLB symptoms than inoculations of rough lemon or 'Valencia'. Inoculations of rough lemon resulted in higher CLas titers and more pronounced HLB symptoms when it was inoculated using infected buds from rough lemon or 'Valencia'. Grafting 'Valencia' with infected buds from Volkamer lemon resulted in less disease than when 'Valencia' was grafted with infected citron, rough lemon, or 'Valencia' buds. Overall, these results suggest that the source of CLas-infected buds used to graft-inoculate some genotypes will influence disease development. Trunk cross-sectional area increase for the year following infection was 3 x higher in 'Kuharske' and rough lemon, compared with 'Valencia'. 'Kuharske' had very low levels of CLas (30 CLas/mu g DNA), whereas 'Valencia' (43,000 CLas/mu g DNA) and rough lemon (6700 CLas/mu g DNA) had relatively high levels. As an alternative to graft-inoculating plants with CLas-infected buds, plants can be subjected to infestations of CLas-infected Asian citrus psyllid (ACP) as occurs naturally. Of interest is if transmission rates of CLas and the development of HLB in a genotype are greater when the ACP have been feeding on the same host genotype. An experiment was therefore conducted to assess transmission of CLas by ACP reared on CLas-infected rough lemon to five different genotypes ('Carrizo', 'Flame' grapefruit, rough lemon, 'Temple', and 'Valencia'). These assessments were made using a detached leaf assay recognized as a faster method of gauging transmission rates of CLas than using whole plants. Higher percentages of ACP died when they were transferred from infected rough lemon to healthy 'Carrizo', and lower percentages died when they were transferred to rough lemon or 'Flame'. However, CLas transmission by infected ACP occurred to at least some leaves of each genotype in each of the five different assays, with 70% or more leaves of each genotype becoming infected in at least one assay. Over all assays, there was relatively little variation among genotypes in the percentage of leaves becoming CLas infected and in the titer of CLas developing in infected leaves. However, there were relatively large differences in transmission rates among individual assays unrelated to differences among test genotypes. Because of the rapidity of the detached leaf assay, efforts are merited to improve consistency of this inoculation method. C1 [Stover, Ed; Hall, David G.; Shatters, Robert G., Jr.] ARS, US Hort Res Lab, USDA, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. [Moore, Gloria A.] Univ Florida, Dept Hort Sci, 1111 Feld Hall,Box 110690, Gainesville, FL 32611 USA. RP Stover, E (reprint author), ARS, US Hort Res Lab, USDA, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM ed.stover@ars.usda.gov FU USDA/ APHIS FX This project was funded in part through USDA/ APHIS. USDA if an equal opportunity provider and employer. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 16 TC 1 Z9 1 U1 3 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD JUL PY 2016 VL 51 IS 7 BP 805 EP 809 PG 5 WC Horticulture SC Agriculture GA DT1KJ UT WOS:000381240900003 ER PT J AU Chen, CX Okie, WR Beckman, TG AF Chen, Chunxian Okie, William R. Beckman, Thomas G. TI Peach Fruit Set and Buttoning after Spring Frost SO HORTSCIENCE LA English DT Article DE Prunus persica; chill requirement; embryo abortion; tree-persisted button; stress-induced trait ID PRUNUS-PERSICA; TOLERANCE; FREEZE; HERITABILITY; CULTIVARS; DEHYDRIN; BLOOM AB Peach fruit set is affected by cumulative chill and spring frost. A spring frost occurred on 29 Mar. 2015 at the U.S. Department of Agriculture, Agricultural Research Service (USDA-ARS) Byron station after 3 weeks of bloom, reducing fruit set and resulting in many buttons (abnormally small fruit with dead embryos). Fruit set was rated in 2014, 2015, and 2016 and button set rated in 2015 using the same scale (0 = no fruit/button to 9 = 1-2 fruit/button at every node). The overall fruit set rating was substantially different in the 3 years, averaging 5.61 in 2014, 2.61 in 2015, and 6.04 in 2016. Buttons and skin-damaged fruit in 2015 varied among peach genotypes. Comparison of fruit and button set ratings showed that there was no difference between cultivars and selections, but some significant differences in fruit set for four ripening months, among the 3 years, and among the nine chilling classes, respectively. Among the cultivars, the most common button set rating was 0-3. For example, 'Sunprince', 'Loring', and 'Carored' trees had a high button set rating, whereas 'Flameprince', 'Julyprince', and 'Contender' trees were low. As for peach selections, BY04P1690n was among those with the highest button set rating. In the population derived from a cross of button-prone BY04P1690n and button-free BY99P3866w, fruit and button counts from 10 long fruiting shoots ranged from 4 to 53 fruit (21.63 on average) and 2 to 27 buttons (10.39 on average). The peach button rate ranged from 5.36% to 87.10% (30.70% on average). The range, distribution, and percentage of the button counts suggested that, if buttoning was genetically controlled, it appeared quantitative. Further assessment is needed. C1 [Chen, Chunxian; Okie, William R.; Beckman, Thomas G.] ARS, USDA, Southeastern Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. RP Chen, CX (reprint author), ARS, USDA, Southeastern Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. EM chunxian.chen@ars.usda.gov FU U.S. Department of Agriculture National Program of Plant Genetic Resources, Genomics and Genetic Improvement [6606-21000-004-006]; Georgia Agriculture Commodity Commission for Peaches Project [58-6042-5-002] FX We thank Bryan Blackburn and Luke Quick for help in maintaining the materials used in this work. The research was partly supported by the U.S. Department of Agriculture National Program of Plant Genetic Resources, Genomics and Genetic Improvement (Project number: 6606-21000-004-006) and Georgia Agriculture Commodity Commission for Peaches (Project number: 58-6042-5-002). This article reports the results of research only. NR 18 TC 0 Z9 0 U1 3 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD JUL PY 2016 VL 51 IS 7 BP 816 EP 821 PG 6 WC Horticulture SC Agriculture GA DT1KJ UT WOS:000381240900005 ER PT J AU Sriladda, C Kratsch, HA Larson, SR Monaco, TA Shen, FA Kjelgren, RK AF Sriladda, Chalita Kratsch, Heidi A. Larson, Steven R. Monaco, Thomas A. Shen, FenAnn Kjelgren, Roger K. TI Interspecific Hybrid of Xeric Shepherdia rotundifolia and Riparian Shepherdia argentea: Description, and Traits Suitable for Low-water Urban Landscapes SO HORTSCIENCE LA English DT Article DE AFLP; SEM; ecophysiology; roundleaf buffaloberry; silver buffaloberry ID MULTILOCUS GENOTYPE DATA; STRESS; INFERENCE; RESPONSES; DROUGHT; DESERT; GROWTH AB Shepherdia rotundifolia Parry (roundleaf buffaloberry), a shrub endemic to the U.S. Colorado Plateau high desert, has aesthetic and drought tolerance qualities desirable for low-water urban landscapes. However, slow growth and too often fatal sensitivity to wet or disturbed soil stymies nursery production and urban landscape use. The goal of this study was to create an interspecific hybrid between the evergreen-xeric S. rotundifolia and its widely adapted, fast-growing, deciduous relative Shepherdia argentea (silver buffaloberry) distributed in western North America riparian habitats. Genetics and leaf morphology of the resulting S. argentea x S. rotundifolia hybrid are described and compared with the parents, as well as hybrid gas exchange as a reasonable proxy for growth rate and potential tolerance of poor soil. Hybrid genotypes were heterogenous, but contained an intermediate and equal contribution of alleles from genetically heterogenous parent populations. Leaf morphology traits were also intermediate between both parents. Aesthetic leaf qualities (silver-blue color and revolute margins) sought from S. rotundifolia were conserved in all offspring. However, gas exchange responses varied widely between the two surviving hybrids. Both hybrids showed greater tolerance of wet, fertile substrate and promise for use in low-water landscapes than S. rotundifolia. However, one hybrid conserved faster growth, and by inference possibly greater tolerance of wet or disturbed soil, from S. argentea, while the opposite was observed in the second hybrid. Following botanical nomenclature, we named this hybrid Shepherdia x utahensis. C1 [Sriladda, Chalita; Kjelgren, Roger K.] Utah State Univ, Dept Plants Soils & Climate, 4820 Old Main Hill, Logan, UT 84322 USA. [Kratsch, Heidi A.] Univ Nevada Cooperat Extens, 4955 Energy Way, Reno, NV 89502 USA. [Larson, Steven R.; Monaco, Thomas A.] Utah State Univ, USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA. [Shen, FenAnn] Utah State Univ, Vice President Res Off, Logan, UT 84322 USA. RP Sriladda, C (reprint author), Utah State Univ, Dept Plants Soils & Climate, 4820 Old Main Hill, Logan, UT 84322 USA. EM sriladda@gmail.com NR 34 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD JUL PY 2016 VL 51 IS 7 BP 822 EP 828 PG 7 WC Horticulture SC Agriculture GA DT1KJ UT WOS:000381240900006 ER PT J AU Xu, CP Mou, BQ AF Xu, Chenping Mou, Beiquan TI Vermicompost Affects Soil Properties and Spinach Growth, Physiology, and Nutritional Value SO HORTSCIENCE LA English DT Article DE Spinacia oleracea; antioxidant; chlorophyll; drench; phytochemicals; soil amendment; worm castings ID HORTICULTURAL POTTING MEDIA; PROCESSED ORGANIC WASTES; HUMIC ACIDS; CHLOROPHYLL FLUORESCENCE; PLANT-GROWTH; PAPER-WASTE; FIELD STRAWBERRIES; SEEDLING GROWTH; CATTLE MANURE; PIG MANURE AB The use of vermicompost to improve soil fertility and enhance crop yield has gained considerable momentum due to its contribution to agroecological sustainability. Short-term (35 days after transplanting) effects of vermicompost, applied either as a soil amendment (5% and 10%, v/v) or a drench (40 mL of vermicompost extract at 0, 14, 21, and 28 days after transplanting), on soil properties and spinach plants (Spinacia oleracea L.) were evaluated in a greenhouse. After harvesting, the amendments left high residual levels of nutrients, organic matter and carbon, and increased soil cation exchange capacity (CEC) and water-holding capacity (WHC). Drench treatment of unamended soil increased soil nutrients, CEC, and WHC. All vermicompost treatments, especially amendment at 10% rate, increased leaf number, area, fresh and dry weight (FW and DW), shoot FW and DW, root DW, and water use efficiency (WUE). Vermicompost increased leaf chlorophyll content, and photochemical efficiency, yield, and electron transport rate (ETR) of mature leaves, as well as increased leaf succulence, and carotenoid, protein, and amino acid content. Vermicompost soil amendment reduced phenolics and flavonoids, leading to lower antioxidant capacity, whereas drench treatment only. decreased betacyanin content. Vermicompost improved soil fertility, prompted leaf production, delayed leaf senescence, and enhanced growth of spinach. It also favorably influenced spinach quality by increasing leaf succulence and carotenoid, protein, and amino acids content, although it, as soil amendment, reduced flavonoid content leading to low antioxidant capacity. C1 [Xu, Chenping; Mou, Beiquan] ARS, USDA, US Agr Res Stn, 1636 East Alisal St, Salinas, CA 93905 USA. RP Xu, CP (reprint author), ARS, USDA, US Agr Res Stn, 1636 East Alisal St, Salinas, CA 93905 USA. EM chenping.xu@ars.usda.gov NR 63 TC 0 Z9 0 U1 10 U2 10 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD JUL PY 2016 VL 51 IS 7 BP 847 EP 855 PG 9 WC Horticulture SC Agriculture GA DT1KJ UT WOS:000381240900010 ER PT J AU Centinari, M Smith, MS Londo, JP AF Centinari, Michela Smith, Maria S. Londo, Jason P. TI Assessment of Freeze Injury of Grapevine Green Tissues in Response to Cultivars and a Cryoprotectant Product SO HORTSCIENCE LA English DT Article DE frost resistance; KDL; osmotic potential; phenological development; Vitis ssp. ID CRITICAL-TEMPERATURES; FROST-RESISTANCE; SPRING FROSTS; LEAF TISSUE; DAMAGE; PROTECTION; TOLERANCE; HARDINESS; SHOOTS; STRESS AB Spring frosts and subsequent crop losses threaten the economic sustainability of fruit crop producers all over the world. This study used a controlled-freezing technique to impose a post-budburst freezing stress to grapevine shoots forced from one-node cuttings ['Albarilio', 'Cabernet Franc', 'Cabernet Sauvignon', and 'Pinot Grigio' (Vitis vinifera)] and whole plants ['Noiret' (Vitis hybrid)]. Our goal was to investigate the incidence of freeze injury among cultivars, stage of phenological development, and a potassium salt-based fertilizer (KDL) with potential cryoprotectant activity. Among the V. vinifera cultivars, the incidence of mortality of shoots exposed to -3.5 degrees C was highest for 'Albarino' (71%) and lowest for 'Cabernet Sauvignon' (51%). Cuttings sprayed with KDL 24 hours before cold temperature exposure exhibited 16% lower shoot mortality and lower osmotic potential (Psi(s)) (-0.92 MPa) than the unsprayed cuttings (-0.77 MPa). However, application of KDL did not impact shoot mortality for whole 'Noiret' vines. Mortality for 'Noiret' shoots greatly increased with the advancement of phenological development, ranging from 10% in wooly buds to 78% in shoots approximate to 10-cm long. The practical significance of KDL remains questionable; cultivar selection still appears to be a more reliable method for avoiding spring frost, by planting late bursting cultivars in more frost-prone areas. C1 [Centinari, Michela; Smith, Maria S.] Penn State Univ, Dept Plant Sci, Tyson Bldg, University Pk, PA 16802 USA. [Londo, Jason P.] ARS, USDA, Grape Genet Res Unit, 630 West North St, Geneva, NY 14456 USA. RP Centinari, M (reprint author), Penn State Univ, Dept Plant Sci, Tyson Bldg, University Pk, PA 16802 USA. EM mzc22@psu.edu NR 39 TC 0 Z9 0 U1 4 U2 4 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD JUL PY 2016 VL 51 IS 7 BP 856 EP 860 PG 5 WC Horticulture SC Agriculture GA DT1KJ UT WOS:000381240900011 ER PT J AU Johnson, LF Cahn, M Martin, F Melton, F Benzen, S Farrara, B Post, K AF Johnson, Lee F. Cahn, Michael Martin, Frank Melton, Forrest Benzen, Sharon Farrara, Barry Post, Kirk TI Evapotranspiration-based Irrigation Scheduling of Head Lettuce and Broccoli SO HORTSCIENCE LA English DT Article DE irrigation management; fractional cover; crop coefficients; Lactuca sativa; Brassica oleracea ID CALIFORNIA; SATELLITE AB Estimation of crop evapotranspiration supports efficient irrigation water management, which in turn supports water conservation, mitigation of groundwater depletion/degradation, energy savings, and crop quality maintenance. Past research in California has revealed strong relationships between fraction of the ground covered by photosynthetically active vegetation (Fc), crop coefficients (Kc), and evapotranspiration (El) of cool-season vegetables and other specialty crops. Replicated irrigation trials for iceberg lettuce and broccoli were performed during 2012 and 2013 at the USDA Agricultural Research Station in Salinas, CA. The main objective was to compare crop yield and quality from ET-based irrigation scheduling with industry standard practice. Sprinkler irrigation was used to germinate and establish the crops, followed by surface drip irrigation during the treatment period. Each experiment compared three irrigation treatment schedules replicated five times in a randomized block design. Two decision-support models were evaluated as follows: 1) anFAO-56-based algorithm embedded in NASA's prototype Satellite Information Management System (SIMS) based on observed Fc, and 2) CropManage (CM), an online database-driven irrigation scheduling tool based on modeled Fc. Both methods used daily reference ETo data from the California Irrigation Management Irrigation System (CIMIS) to translate Kc to crop ET, with a target of 100% replacement of water use during the drip irrigation phase. A third treatment followed an irrigation schedule representing grower standard practice (SP) at 150% to 175% ET replacement during the drip irrigation phase. No significant treatment differences were seen in lettuce head weight or total biomass. Marketable yields of lettuce (near 45.4 Mg.ha(-1)) and broccoli (near 17.4 Mg.ha(-1)) were in-line with industry averages during both years and all treatments. During 2012, CM yield was below lettuce SP, and above broccoli SP, while in 2013 no treatment differences were detected for either crop. No significant differences were detected between SIMS and SP yields during any trial. C1 [Johnson, Lee F.; Melton, Forrest; Post, Kirk] Calif State Univ, Div Sci & Environm Policy, Seaside, CA 93955 USA. [Johnson, Lee F.; Melton, Forrest] NASA, Ames Res Ctr, Div Earth Sci, Moffett Field, CA 94035 USA. [Cahn, Michael; Farrara, Barry] Univ Calif Cooperat Extens, Salinas, CA 93901 USA. [Martin, Frank; Benzen, Sharon] ARS, USDA, Salinas, CA 93905 USA. RP Johnson, LF (reprint author), Calif State Univ, Div Sci & Environm Policy, Seaside, CA 93955 USA.; Johnson, LF (reprint author), NASA, Ames Res Ctr, Div Earth Sci, Moffett Field, CA 94035 USA. EM lee.f.johnson@nasa.gov FU California Dept. Food Agriculture [SCB11016]; NASA's Applied Sciences Program FX The project was sponsored by the California Dept. Food & Agriculture no. SCB11016 with additional support from NASA's Applied Sciences Program. Commercial cooperators Fresh Express and Tanimura & Antle harvested the crops and evaluated, or provided guidance on collection of, associated crop quality data. We thank Sakata, Enza, and Seminis for donating seeds, and Wilbur-Ellis for fertilizer. David Lara and Gerry Ochoa (USDA-ARS) contributed to agricultural operations. NR 22 TC 1 Z9 1 U1 3 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD JUL PY 2016 VL 51 IS 7 BP 935 EP 940 PG 6 WC Horticulture SC Agriculture GA DT1KJ UT WOS:000381240900023 ER PT J AU Park, EY Fuerst, EP Baik, BK AF Park, Eun Young Fuerst, E. Patrick Baik, Byung-Kee TI Phytate negatively influences wheat dough and bread characteristics by interfering with cross-linking of glutenin molecules SO JOURNAL OF CEREAL SCIENCE LA English DT Article DE Phytate; Chelating capacity; Dough property; Bread quality ID PHYTIC ACID; QUALITY PARAMETERS; MIXING PROPERTIES; FUNGAL PHYTASE; PROTEINS; SIZE; FERMENTATION; TEMPERATURE; PERFORMANCE; FOODS AB The influence of added phytate on dough properties and bread baking quality was studied to determine the role of phytate in the impaired functional properties of whole grain wheat flour for baking bread. Phytate addition to refined flour at a 1% level substantially increased mixograph mixing time, generally increased mixograph water absorption, and reduced the SDS-unextractable protein content of dough before and after fermentation as well as the loaf volume of bread. The added phytate also shifted unextractable glutenins toward a lower molecular weight form and increased the iron-chelating activity of dough. It appears that phytate negatively affects gluten development and loaf volume by chelating iron and/or binding glutenins, and consequently interfering with the oxidative cross-linking of glutenin molecules during dough mixing. Phytate could be at least partially responsible for the weak gluten network and decreased loaf volume of whole wheat flour bread as compared to refined flour bread. Published by Elsevier Ltd. C1 [Park, Eun Young] Washington State Univ, Sch Food Sci, Pullman, WA 99164 USA. [Fuerst, E. Patrick] Washington State Univ, Western Wheat Qual Lab, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Baik, Byung-Kee] ARS, USDA, CSWQRU, Wooster, OH 44691 USA. RP Baik, BK (reprint author), ARS, USDA, CSWQRU, Wooster, OH 44691 USA.; Baik, BK (reprint author), Korea Univ, Coll Life Sci & Biotechnol, Dept Biotechnol, Seoul 02841, South Korea. EM byungkee.baik@ars.usda.gov NR 33 TC 0 Z9 0 U1 7 U2 7 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0733-5210 EI 1095-9963 J9 J CEREAL SCI JI J. Cereal Sci. PD JUL PY 2016 VL 70 BP 199 EP 206 DI 10.1016/j.jcs.2016.06.012 PG 8 WC Food Science & Technology SC Food Science & Technology GA DU1EI UT WOS:000381950600028 ER PT J AU Cottrell, TE Ree, W AF Cottrell, Ted E. Ree, William TI Assays of Orchard-Applied Insecticides on the Brown Stink Bug (Hemiptera: Pentatomidae) Feeding on Pecan SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE Carya illinoinensis; feces rating; insecticide label; residual activity ID GLASS-VIAL BIOASSAY; EUSCHISTUS-SERVUS; ADULT BROWN; ACROSTERNUM-HILARE; STYLET SHEATH; TRAP CAPTURE; TOXICITY; HETEROPTERA; GREEN; INDICATOR AB The brown stink bug, Euschistus servus (Say) (Hemiptera: Pentatomidae), is an economic pest of pecan, Carya illinoinensis (Wangenh.) K. Koch (Juglandaceae), and other agronomic crops across the southeastern United States. The efficacy of many insecticides against E. servus attacking pecan is not well documented even though the label for many of these insecticides may indicate one or more stink bug species as targets. Thus, we assayed various insecticides, labeled for application to pecan, to determine efficacy against E. servus. Insecticides were applied to pecan limb terminals with nut clusters in orchards in Georgia and Texas during 2009 and 2010. Treated limb terminals were cut from the tree and taken to the laboratory 1, 4, and 7 d after treatment. Treated nuts or limb terminals (i.e., nuts and foliage) were placed individually in cups or cylinder cages, respectively, and adult E. servus then placed into these containers. Mortality and moribundity of E. servus were recorded at 24, 48, or 72 h in addition to rating feces production at 72 h in some trials. Results consistently indicate that bifenthrin provided greater control of adult E. servus for a longer time after application than other products, including the organophosphate chlorpyrifos. Aside from bifenthrin also affecting the feces rating, the only other treatment that reduced feces production compared with untreated nuts was lambda-cyhalothrin + thiamethoxam. C1 [Cottrell, Ted E.; Ree, William] ARS, USDA, Southeastern Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. [Ree, William] Texas A&M Univ, Texas A&M AgriLife Extens, POB 2150, Bryan, TX 77806 USA. RP Cottrell, TE (reprint author), ARS, USDA, Southeastern Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. EM ted.cottrell@ars.usda.gov FU Georgia Agricultural Commodity Commission for Pecan FX The authors thank A. Amis and S. Starks (USDA, ARS, SEFTNRL, Byron, GA) for technical assistance. A portion of the research reported here was funded by the Georgia Agricultural Commodity Commission for Pecan. The United States Government has the right to retain a non-exclusive, royalty-free license in and to any copyright of this article. This article reports the results of research only. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. NR 30 TC 0 Z9 0 U1 2 U2 2 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD JUL PY 2016 VL 51 IS 3 BP 179 EP 198 PG 20 WC Entomology SC Entomology GA DT4KG UT WOS:000381448000001 ER PT J AU Ni, XZ Cottrell, TE Toews, MD Tillman, PG Buntin, GD AF Ni, Xinzhi Cottrell, Ted E. Toews, Michael D. Tillman, P. Glynn Buntin, G. David TI Diurnal Activities of the Brown Stink Bug (Hemiptera: Pentatomidae) in and near Tasseling Corn Fields SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE Euschistus servus (Say); diurnal movement; mark-release-recapture; pheromone-baited trap ID HETEROPTERA-PENTATOMIDAE; EUSCHISTUS-SERVUS; SOUTH-CAROLINA; COTTON; ABUNDANCE; DYNAMICS AB The demand for effective management of the brown stink bug, Euschistus servus (Say), in corn and other crops has been increasing in recent years. To identify when and where the stink bugs are most likely to occur for targeted insecticide application, diurnal activities of stink bugs in and near the fields of tasseling corn plants were monitored using pheromone traps. Trap capture of the brown stink bugs in the corn field showed that the number of females was greater at corn field edges than in the interior, but there was no difference in male capture between the edge and interior. Stink bug dispersal within corn fields was further monitored using a mark release recapture technique, but the technique was not effective because only one of 158 marked individuals was recovered in the pheromone trap. To further understand the movement pattern among host plants, brown stink bugs were monitored continuously for 6 d near the tasseling corn fields using the pheromone traps. The number of females caught in pheromone traps near the corn fields in early mornings and evenings was greater than in the midafternoon. Within each sampling time across the 6-d monitoring period, total number and number of each sex caught in early mornings were greater on the first 2 d when compared to the remaining 4-d monitoring period. The implications of the findings for brown stink bug management are discussed. C1 [Ni, Xinzhi; Cottrell, Ted E.; Toews, Michael D.; Tillman, P. Glynn; Buntin, G. David] ARS, USDA, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. [Cottrell, Ted E.] USDA ARS, Southeastern Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. [Toews, Michael D.] Univ Georgia, Dept Entomol, Tifton Campus, Tifton, GA 31793 USA. [Tillman, P. Glynn] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. [Buntin, G. David] Univ Georgia, Dept Entomol, Griffin Campus, Griffin, GA 30223 USA. RP Ni, XZ (reprint author), ARS, USDA, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. EM xinzhi.ni@ars.usda.gov FU Georgia Agricultural Commodity Commission for Corn FX The research was supported in part by the Georgia Agricultural Commodity Commission for Corn. We appreciate the assistance of A. Amis (USDA-ARS, Southeastern Fruit and Tree Nut Research Laboratory, Byron, GA), J.C. Mullis and P. Tapp (USDA-ARS, Crop Genetics and Breeding Research Unit, Tifton, GA), and K. Graham (USDA-ARS, Crop Protection and Management Research Unit, Tifton, GA) for technical assistance and D.G. Riley, P.M. Roberts, and A.N. Sparks, Jr. (Department of Entomology, University of Georgia) for their constructive reviews of the manuscript. NR 20 TC 0 Z9 0 U1 2 U2 2 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD JUL PY 2016 VL 51 IS 3 BP 226 EP 237 PG 12 WC Entomology SC Entomology GA DT4KG UT WOS:000381448000005 ER PT J AU Cheng, SJ Steiner, AL Hollinger, DY Bohrer, G Nadelhoffer, KJ AF Cheng, S. J. Steiner, A. L. Hollinger, D. Y. Bohrer, G. Nadelhoffer, K. J. TI Using satellite-derived optical thickness to assess the influence of clouds on terrestrial carbon uptake SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article DE cloud optical thickness; ecosystem productivity; MODIS; diffuse light ID LIGHT-USE EFFICIENCY; SOLAR-RADIATION MEASUREMENTS; SINGLE-SCATTERING PROPERTIES; NET ECOSYSTEM EXCHANGE; DIFFUSE-RADIATION; DIOXIDE EXCHANGE; WATER-VAPOR; MULTIPLE-SCATTERING; DECIDUOUS FOREST; SKY CONDITIONS AB Clouds scatter direct solar radiation, generating diffuse radiation and altering the ratio of direct to diffuse light. If diffuse light increases plant canopy CO2 uptake, clouds may indirectly influence climate by altering the terrestrial carbon cycle. However, past research primarily uses proxies or qualitative categories of clouds to connect the effect of diffuse light on CO2 uptake to sky conditions. We mechanistically link and quantify effects of cloud optical thickness ((c)) to surface light and plant canopy CO2 uptake by comparing satellite retrievals of (c) to ground-based measurements of diffuse and total photosynthetically active radiation (PAR; 400-700nm) and gross primary production (GPP) in forests and croplands. Overall, total PAR decreased with (c), while diffuse PAR increased until an average (c) of 6.8 and decreased with larger (c). When diffuse PAR increased with (c), 7-24% of variation in diffuse PAR was explained by (c). Light-use efficiency (LUE) in this range increased 0.001-0.002 per unit increase in (c). Although (c) explained 10-20% of the variation in LUE, there was no significant relationship between (c) and GPP (p>0.05) when diffuse PAR increased. We conclude that diffuse PAR increases under a narrow range of optically thin clouds and the dominant effect of clouds is to reduce total plant-available PAR. This decrease in total PAR offsets the increase in LUE under increasing diffuse PAR, providing evidence that changes within this range of low cloud optical thickness are unlikely to alter the magnitude of terrestrial CO2 fluxes. C1 [Cheng, S. J.; Nadelhoffer, K. J.] Univ Michigan, Dept Ecol & Evolutionary Biol, Ann Arbor, MI 48109 USA. [Steiner, A. L.] Univ Michigan, Dept Climate & Space Sci & Engn, Ann Arbor, MI 48109 USA. [Hollinger, D. Y.] US Forest Serv, Northern Res Stn, USDA, Durham, NH USA. [Bohrer, G.] Ohio State Univ, Dept Civil Environm & Geodet Engn, Columbus, OH 43210 USA. RP Cheng, SJ (reprint author), Univ Michigan, Dept Ecol & Evolutionary Biol, Ann Arbor, MI 48109 USA. EM chengs@umich.edu FU University of Michigan Department of Ecology and Evolutionary Biology Brower Fellowship; Michigan Space Grant Consortium; NSF AGS [1242203]; NSF [1521238]; U.S. Department of Energy's Office of Science; North American Carbon Program/USDA CREES NRI [2004-35111-15057, 2008-35101- 19076]; Science Foundation Arizona [CAA 0-203-08]; USDA Forest Service Northern Research Station FX We acknowledge and thank the following AmeriFlux sites for their data records: US-Ho1, US-Ne1, US-MMS, US-UMB, US-Bar, US-Fuf, US-Snd, and US-Var. We also thank Dori Mermelstein and Stacey Kawecki for their help with NCL scripts, the University of Michigan Center for Statistical Consulting and Research for statistical advisement, and Paul Hubanks and Gala Wind for responding to questions about MODIS data. We also appreciate the thoughtful comments from two anonymous reviewers, Bill Currie, Peter Curtis, Deborah Goldberg, and Gretchen Keppel-Aleks that strengthened the quality of this paper. Funding for S.J.C. was provided in part by the University of Michigan Department of Ecology and Evolutionary Biology Brower Fellowship and the Michigan Space Grant Consortium. A.L.S. was supported in part by NSF AGS 1242203. G.B. was funded in part by NSF 1521238. Funding for AmeriFlux data resources and for core site (US-Ho1, US-Ne1, US-UMB, and US-MMS) data was provided by the U.S. Department of Energy's Office of Science. Data from US-Fuf were also funded by grants from the North American Carbon Program/USDA CREES NRI (2004-35111-15057 and 2008-35101- 19076) and Science Foundation Arizona (CAA 0-203-08) awarded to T. Kolb at Northern Arizona University. Additional support for US-Ho1 was provided by the USDA Forest Service Northern Research Station. The datawe used in this paper can be found through the AmeriFlux Network (http://ameriflux.lbl.gov/) and NASA's Level 1 and Atmosphere Archive and Distribution System (https://ladsweb.nascom.nasa.gov/data/). NR 76 TC 1 Z9 1 U1 5 U2 5 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD JUL PY 2016 VL 121 IS 7 BP 1747 EP 1761 DI 10.1002/2016JG003365 PG 15 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA DV0BG UT WOS:000382581900003 ER PT J AU Corcoran, MP Chui, KKH White, DK Reid, KF Kirn, D Nelson, ME Sacheck, JM Folta, SC Fielding, RA AF Corcoran, M. P. Chui, K. K. H. White, D. K. Reid, K. F. Kirn, D. Nelson, M. E. Sacheck, J. M. Folta, S. C. Fielding, R. A. TI Accelerometer assessment of physical activity and its association with physical function in older adults residing at assisted care facilities SO JOURNAL OF NUTRITION HEALTH & AGING LA English DT Article DE Physical activity; physical function; older adults; assisted care facilities ID PERFORMANCE; MORTALITY; DISABILITY; MOBILITY; INTERVENTIONS; LIMITATION; STEPS/DAY; PEOPLE; RISK AB To describe levels of physical activity among older adults residing at assisted care facilities and their association with physical function. Cross-sectional analysis. Assisted care facilities within the greater Boston, MA area. Older adults aged 65 years and older (N = 65). Physical Activity Level (PAL) as defined by quartiles from accelerometry (counts and steps), Short Physical Performance Battery (SPPB) Score, gait speed, and handgrip strength. Participants in the most active accelerometry quartile engaged in 25 minutes/week of moderate to vigorous physical activity (MVPA) and walked 2,150 steps/day. These individuals had an SPPB score, 400 meter walk speed, and handgrip strength that was 3.7-3.9 points, 0.3-0.4 meters/second, and 4.5-5.1 kg greater respectively, than individuals in the lowest activity quartile, who engaged in less than 5 min/wk of MVPA or took fewer than 460 steps/day. Despite engaging in physical activity levels far below current recommendations (150 min/week of MVPA or > 7000 steps/day), the most active older adults in this study exhibited clinically significant differences in physical function relative to their less active peers. While the direction of causality cannot be determined from this cross-sectional study, these findings suggest a strong association between PAL and physical function among older adults residing in an assisted care facility. C1 [Corcoran, M. P.; Nelson, M. E.; Sacheck, J. M.; Folta, S. C.; Fielding, R. A.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Chui, K. K. H.] Tufts Univ, Sch Med, Boston, MA 02111 USA. [White, D. K.] Univ Delaware, Dept Phys Therapy, Newark, DE USA. [Reid, K. F.; Kirn, D.; Nelson, M. E.; Sacheck, J. M.; Folta, S. C.; Fielding, R. A.] Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Corcoran, MP (reprint author), Merrimack Coll, Hlth Sci, N Andover, MA 01845 USA. EM corcoranm@merrimack.edu FU Nestle Health Science, Vevey, Switzerland [09.37.CLI] FX This work was supported by Nestle Health Science, Vevey, Switzerland (Trial No. 09.37.CLI). NR 33 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 1279-7707 EI 1760-4788 J9 J NUTR HEALTH AGING JI J. Nutr. Health Aging PD JUL PY 2016 VL 20 IS 7 BP 752 EP 758 DI 10.1007/s12603-015-0640-7 PG 7 WC Geriatrics & Gerontology; Nutrition & Dietetics SC Geriatrics & Gerontology; Nutrition & Dietetics GA DT6FY UT WOS:000381580300011 PM 27499309 ER PT J AU Ko, DK Rohozinski, D Song, QX Taylor, SH Juenger, TE Harmon, FG Chen, ZJ AF Ko, Dae Kwan Rohozinski, Dominica Song, Qingxin Taylor, Samuel H. Juenger, Thomas E. Harmon, Frank G. Chen, Z. Jeffrey TI Temporal Shift of Circadian-Mediated Gene Expression and Carbon Fixation Contributes to Biomass Heterosis in Maize Hybrids SO PLOS GENETICS LA English DT Article ID ARABIDOPSIS-THALIANA; INTRASPECIFIC HYBRIDS; CLOCK REGULATION; FLOWERING TIME; GROWTH VIGOR; F-1 HYBRID; PLANTS; RHYTHMS; PHOTOSYNTHESIS; TRANSFORMATION AB Heterosis has been widely used in agriculture, but the molecular mechanism for this remains largely elusive. In Arabidopsis hybrids and allopolyploids, increased photosynthetic and metabolic activities are linked to altered expression of circadian clock regulators, including CIRCADIAN CLOCK ASSOCIATED1 (CCA1). It is unknown whether a similar mechanism mediates heterosis in maize hybrids. Here we report that higher levels of carbon fixation and starch accumulation in the maize hybrids are associated with altered temporal gene expression. Two maize CCA1 homologs, ZmCCA1a and ZmCCA1b, are diurnally up-regulated in the hybrids. Expressing ZmCCA1 complements the cca1 mutant phenotype in Arabidopsis, and overexpressing ZmCCA1b disrupts circadian rhythms and biomass heterosis. Furthermore, overexpressing ZmCCA1b in maize reduced chlorophyll content and plant height. Reduced height stems from reduced node elongation but not total node number in both greenhouse and field conditions. Phenotypes are less severe in the field than in the greenhouse, suggesting that enhanced light and/or metabolic activities in the field can compensate for altered circadian regulation in growth vigor. Chromatin immunoprecipitation-sequencing (ChIP-seq) analysis reveals a temporal shift of ZmCCA1-binding targets to the early morning in the hybrids, suggesting that activation of morning-phased genes in the hybrids promotes photosynthesis and growth vigor. This temporal shift of ZmCCA1-binding targets correlated with nonadditive and additive gene expression in early and late stages of seedling development. These results could guide breeding better hybrid crops to meet the growing demand in food and bioenergy. C1 [Ko, Dae Kwan; Song, Qingxin; Chen, Z. Jeffrey] Univ Texas Austin, Dept Mol Biosci, Austin, TX 78712 USA. [Rohozinski, Dominica; Harmon, Frank G.] ARS, Ctr Plant Gene Express, USDA, Albany, CA USA. [Rohozinski, Dominica; Harmon, Frank G.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Taylor, Samuel H.; Juenger, Thomas E.; Chen, Z. Jeffrey] Univ Texas Austin, Ctr Computat Biol & Bioinformat, Dept Integrat Biol, Austin, TX 78712 USA. [Taylor, Samuel H.; Juenger, Thomas E.; Chen, Z. Jeffrey] Univ Texas Austin, Inst Cellular & Mol Biol, Austin, TX 78712 USA. RP Chen, ZJ (reprint author), Univ Texas Austin, Dept Mol Biosci, Austin, TX 78712 USA.; Chen, ZJ (reprint author), Univ Texas Austin, Ctr Computat Biol & Bioinformat, Dept Integrat Biol, Austin, TX 78712 USA.; Chen, ZJ (reprint author), Univ Texas Austin, Inst Cellular & Mol Biol, Austin, TX 78712 USA. EM zjchen@austin.utexas.edu OI Taylor, Samuel/0000-0001-9714-0656 FU National Science Foundation (USA) [IOS1238048] FX This work was funded by National Science Foundation (USA) (grant IOS1238048). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 89 TC 0 Z9 0 U1 16 U2 16 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7404 J9 PLOS GENET JI PLoS Genet. PD JUL PY 2016 VL 12 IS 7 AR e1006197 DI 10.1371/journal.pgen.1006197 PG 31 WC Genetics & Heredity SC Genetics & Heredity GA DS8RI UT WOS:000381050100054 PM 27467757 ER PT J AU Coy, A Rankine, D Taylor, M Nielsen, DC Cohen, J AF Coy, Andre Rankine, Dale Taylor, Michael Nielsen, David C. Cohen, Jane TI Increasing the Accuracy and Automation of Fractional Vegetation Cover Estimation from Digital Photographs SO REMOTE SENSING LA English DT Article DE fractional vegetation cover; automated canopy estimation; unsupervised image segmentation; digital photographs ID LEAF-AREA INDEX; IMAGE-ANALYSIS; GROUND-COVER; CANOPY COVER; SEGMENTATION; ALGORITHM; WHEAT; VALIDATION; SYSTEM; AERIAL AB The use of automated methods to estimate fractional vegetation cover (FVC) from digital photographs has increased in recent years given its potential to produce accurate, fast and inexpensive FVC measurements. Wide acceptance has been delayed because of the limitations in accuracy, speed, automation and generalization of these methods. This work introduces a novel technique, the Automated Canopy Estimator (ACE) that overcomes many of these challenges to produce accurate estimates of fractional vegetation cover using an unsupervised segmentation process. ACE is shown to outperform nine other segmentation algorithms, consisting of both threshold-based and machine learning approaches, in the segmentation of photographs of four different crops (oat, corn, rapeseed and flax) with an overall accuracy of 89.6%. ACE is similarly accurate (88.7%) when applied to remotely sensed corn, producing FVC estimates that are strongly correlated with ground truth values. C1 [Coy, Andre; Rankine, Dale; Taylor, Michael] Univ West Indies, Dept Phys, Mona, Jamaica. [Nielsen, David C.] USDA ARS, Cent Great Plains Res Stn, Akron, CO 80720 USA. [Cohen, Jane] Univ West Indies, Dept Life Sci, Mona, Jamaica. RP Coy, A (reprint author), Univ West Indies, Dept Phys, Mona, Jamaica. EM andre.coy@gmail.com; dralstonrankine@gmail.com; michael.taylor@uwimona.edu.jm; david.nielsen@ars.usda.gov; jane.cohen@uwimona.edu.jm RI Nielsen, David/A-8044-2009; OI Nielsen, David/0000-0002-8240-7183; Cohen, Jane/0000-0003-4933-6106 FU European Union FX This research has been produced with the financial assistance of the European Union. The contents of this document are the sole responsibility of the University of the West Indies and its partners in the Global-Local Caribbean Climate Change Adaptation and Mitigation Scenarios (GoLoCarSce) project and can under no circumstances be regarded as reflecting the position of the European Union. The authors would also like to thank X.D. Bai and Xihan Mu for providing their algorithms for comparison and K.C. DeJonge for providing the remotely sensed corn images. The authors would also like to thank the anonymous reviewers for their comments and suggestions, which have served to significantly improve the paper. NR 43 TC 0 Z9 0 U1 14 U2 14 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD JUL PY 2016 VL 8 IS 7 AR 474 DI 10.3390/rs8070474 PG 14 WC Remote Sensing SC Remote Sensing GA DU5AR UT WOS:000382224800001 ER PT J AU Kang, YH Ozdogan, M Zipper, SC Roman, MO Walker, J Hong, SY Marshall, M Magliulo, V Moreno, J Alonso, L Miyata, A Kimball, B Loheide, SP AF Kang, Yanghui Ozdogan, Mutlu Zipper, Samuel C. Roman, Miguel O. Walker, Jeff Hong, Suk Young Marshall, Michael Magliulo, Vincenzo Moreno, Jose Alonso, Luis Miyata, Akira Kimball, Bruce Loheide, Steven P., II TI How Universal Is the Relationship between Remotely Sensed Vegetation Indices and Crop Leaf Area Index? A Global Assessment SO REMOTE SENSING LA English DT Article DE LAI; Vegetation Index; agriculture; Landsat; agroecosystem modeling ID PHOTOSYNTHETICALLY ACTIVE RADIATION; ESSENTIAL CLIMATE VARIABLES; ABSOLUTE ERRORS REGRESSION; CANOPY CHLOROPHYLL CONTENT; NET PRIMARY PRODUCTION; GREEN LAI; TRANSFER MODELS; TIME-SERIES; EOS-MODIS; HEMISPHERICAL PHOTOGRAPHY AB Leaf Area Index (LAI) is a key variable that bridges remote sensing observations to the quantification of agroecosystem processes. In this study, we assessed the universality of the relationships between crop LAI and remotely sensed Vegetation Indices (VIs). We first compiled a global dataset of 1459 in situ quality-controlled crop LAI measurements and collected Landsat satellite images to derive five different VIs including Simple Ratio (SR), Normalized Difference Vegetation Index (NDVI), two versions of the Enhanced Vegetation Index (EVI and EVI2), and Green Chlorophyll Index (CIGreen). Based on this dataset, we developed global LAI-VI relationships for each crop type and VI using symbolic regression and Theil-Sen (TS) robust estimator. Results suggest that the global LAI-VI relationships are statistically significant, crop-specific, and mostly non-linear. These relationships explain more than half of the total variance in ground LAI observations (R-2 > 0.5), and provide LAI estimates with RMSE below 1.2 m(2)/m(2). Among the five VIs, EVI/EVI2 are the most effective, and the crop-specific LAI-EVI and LAI-EVI2 relationships constructed by TS, are robust when tested by three independent validation datasets of varied spatial scales. While the heterogeneity of agricultural landscapes leads to a diverse set of local LAI-VI relationships, the relationships provided here represent global universality on an average basis, allowing the generation of large-scale spatial-explicit LAI maps. This study contributes to the operationalization of large-area crop modeling and, by extension, has relevance to both fundamental and applied agroecosystem research. C1 [Kang, Yanghui; Ozdogan, Mutlu] Univ Wisconsin, Nelson Inst Ctr Sustainabil & Global Environm, 1710 Univ Ave, Madison, WI 53726 USA. [Kang, Yanghui] Univ Wisconsin, Dept Geog, Madison, WI 53706 USA. [Zipper, Samuel C.; Loheide, Steven P., II] Univ Wisconsin, Dept Civil & Environm Engn, Madison, WI 53706 USA. [Roman, Miguel O.] NASA, Goddard Space Flight Ctr, Terr Informat Syst Lab, Code 619 Bld 32 S-036F, Greenbelt, MD 20771 USA. [Walker, Jeff] Monash Univ, Dept Civil Engn, Clayton, Vic 3800, Australia. [Hong, Suk Young] RDA, Natl Inst Agr Sci NAS, Agr Environm Dept, Wonju 55365, South Korea. [Marshall, Michael] World Agroforestry Ctr, Climate Res Unit, United Nations Ave,POB 30677, Nairobi 00100, Kenya. [Magliulo, Vincenzo] CNR, Inst Mediterranean Agr & Forest Syst, CNR ISAFOM, Via Patacca 85, I-80040 Naples, Italy. [Magliulo, Vincenzo] Natl Res Council IBIMET CNR, Inst Biometeorol, I-50145 Florence 8, Italy. [Moreno, Jose; Alonso, Luis] Univ Valencia, Dept Earth Phys & Thermodynam, Lab Earth Observat, E-46100 Valencia, Spain. [Miyata, Akira] NARO, Inst Agroenvironm Sci, Tsukuba, Ibaraki 3058604, Japan. [Kimball, Bruce] ARS, US Arid Land Agr Res Ctr, USDA, Maricopa, AZ 85138 USA. RP Ozdogan, M (reprint author), Univ Wisconsin, Nelson Inst Ctr Sustainabil & Global Environm, 1710 Univ Ave, Madison, WI 53726 USA. EM ykang38@wisc.edu; ozdogan@wisc.edu; szipper@wisc.edu; miguel.o.roman@nasa.gov; jeff.walker@monash.edu; syhong67@korea.kr; m.marshall@cgiar.org; enzo.magliulo@cnr.it; Jose.Moreno@uv.es; luis.alonso@uv.es; amiyat@affrc.go.jp; Bruce.Kimball@ars.usda.gov; loheide@wisc.edu OI Kang, Yanghui/0000-0001-8563-1503; Zipper, Samuel/0000-0002-8735-5757 FU NASA Earth and Space Science Fellowship; National Science Foundation Water Sustainability & Climate Program [DEB-1038759]; North Temperate Lakes Long-Term Ecological Research Program [DEB-0822700]; University of Wisconsin-Madison Anna Grant Birge Award FX Yanghui Kang was supported by NASA Earth and Space Science Fellowship. Samuel C. Zipper, Steven P. Loheide and Wisconsin data collection were supported by the National Science Foundation Water Sustainability & Climate Program (DEB-1038759), the North Temperate Lakes Long-Term Ecological Research Program (DEB-0822700), and the University of Wisconsin-Madison Anna Grant Birge Award. Jeff Oimoen, Eric Booth, Melissa Motew, and the Wisconsin Department of Natural Resources assisted in the collection of aerial imagery. We would also like to thank the Wisconsin land owner who provided access to his fields for field-scale validation data collection. NR 113 TC 0 Z9 0 U1 21 U2 21 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD JUL PY 2016 VL 8 IS 7 AR 597 DI 10.3390/rs8070597 PG 29 WC Remote Sensing SC Remote Sensing GA DU5AR UT WOS:000382224800067 ER PT J AU Wallace, CSA Walker, JJ Skirvin, SM Patrick-Birdwell, C Weltzin, JF Raichle, H AF Wallace, Cynthia S. A. Walker, Jessica J. Skirvin, Susan M. Patrick-Birdwell, Caroline Weltzin, Jake F. Raichle, Helen TI Mapping Presence and Predicting Phenological Status of Invasive Buffelgrass in Southern Arizona Using MODIS, Climate and Citizen Science Observation Data SO REMOTE SENSING LA English DT Article DE buffelgrass; MODIS-NDVI; PRISM; phenology; correlation phenometrics; invasive species ID SONORAN DESERT; PENNISETUM-CILIARE; GLOBAL CHANGE; LAND-COVER; GRASS; PLANT; PRODUCTIVITY; VARIABILITY; MANAGEMENT; CHEATGRASS AB The increasing spread and abundance of an invasive perennial grass, buffelgrass (Pennisetum ciliare), represents a critical threat to the native vegetation communities of the Sonoran desert in southern Arizona, USA, where buffelgrass eradication is a high priority for resource managers. Herbicidal treatment of buffelgrass is most effective when the vegetation is actively growing, but the remoteness of infestations and the erratic timing and length of the species' growth periods confound effective treatment. The goal of our research is to promote buffelgrass management by using remote sensing data to detect where the invasive plants are located and when they are photosynthetically active. We integrated citizen scientist observations of buffelgrass phenology in the Tucson, Arizona area with PRISM precipitation data, eight-day composites of 250-m Moderate-resolution Imaging Spectroradiometer (MODIS) satellite imagery, and aerially-mapped polygons of buffelgrass presence to understand dynamics and relationships between precipitation and the timing and amount of buffelgrass greenness from 2011 to 2013. Our results show that buffelgrass responds quickly to antecedent rainfall: in pixels containing buffelgrass, higher correlations (R-2 > 0.5) typically occur after two cumulative eight-day periods of rain, whereas in pixels dominated by native vegetation, four prior 8-day periods are required to reach that threshold. Using the new suite of phenometrics introduced here-Climate Landscape Response metrics-we accurately predicted the location of 49% to 55% of buffelgrass patches in Saguaro National Park. These metrics and the suggested guidelines for their use can be employed by resource managers to treat buffelgrass during optimal time periods. C1 [Wallace, Cynthia S. A.; Walker, Jessica J.] US Geol Survey, Western Geog Sci Ctr, Tucson, AZ 85719 USA. [Skirvin, Susan M.] USDA ARS, Tucson, AZ 85719 USA. [Patrick-Birdwell, Caroline] Southern Arizona Buffelgrass Coordinat Ctr, Tucson, AZ 85717 USA. [Weltzin, Jake F.] US Geol Survey, USA Natl Phenol Network, Tucson, AZ 85721 USA. [Raichle, Helen] Southwest Biol Sci Ctr, Tucson, AZ 85719 USA. RP Wallace, CSA (reprint author), US Geol Survey, Western Geog Sci Ctr, Tucson, AZ 85719 USA. EM cwallace@usgs.gov; jjwalker@usgs.gov; smskirvin@gmail.com; happydesert@gmail.com; jweltzin@usgs.gov; raichlehelen@yahoo.com OI Weltzin, Jake/0000-0001-8641-6645 FU USGS (Land Change Science); USGS (Land Remote Sensing); USGS (National Park Monitoring Project within the Status & Trends Program); USGS Mendenhall Research Fellowship FX We wish to thank the National Park Service and Dana Backer, in particular, for supplying us with data and critical feedback on the methods used here. Data were provided by the USA National Phenology Network and the many participants who contribute to its Nature's Notebook program. The USGS (Land Change Science, Land Remote Sensing and National Park Monitoring Project within the Status & Trends Program) provided funding for this research. Jessica Walker was supported by a USGS Mendenhall Research Fellowship during this work. We appreciate the thoughtful and constructive comments from our three anonymous reviewers and USGS internal reviewer Joel Sankey. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 35 TC 0 Z9 0 U1 12 U2 12 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD JUL PY 2016 VL 8 IS 7 AR 524 DI 10.3390/rs8070524 PG 24 WC Remote Sensing SC Remote Sensing GA DU5AR UT WOS:000382224800002 ER PT J AU Mansouri, K Abdelaziz, A Rybacka, A Roncaglioni, A Tropsha, A Varnek, A Zakharov, A Worth, A Richard, AM Grulke, CM Trisciuzzi, D Fourches, D Horvath, D Benfenati, E Muratov, E Wedebye, EB Grisoni, F Mangiatordi, GF Incisivo, GM Hong, HX Ng, HW Tetko, IV Balabin, I Kancherla, J Shen, J Burton, J Nicklaus, M Cassotti, M Nikolov, NG Nicolotti, O Andersson, PL Zang, QD Politi, R Beger, RD Todeschini, R Huang, RL Farag, S Rosenberg, SA Slavov, S Hu, X Judson, RS AF Mansouri, Kamel Abdelaziz, Ahmed Rybacka, Aleksandra Roncaglioni, Alessandra Tropsha, Alexander Varnek, Alexandre Zakharov, Alexey Worth, Andrew Richard, Ann M. Grulke, Christopher M. Trisciuzzi, Daniela Fourches, Denis Horvath, Dragos Benfenati, Emilio Muratov, Eugene Wedebye, Eva Bay Grisoni, Francesca Mangiatordi, Giuseppe F. Incisivo, Giuseppina M. Hong, Huixiao Ng, Hui W. Tetko, Igor V. Balabin, Ilya Kancherla, Jayaram Shen, Jie Burton, Julien Nicklaus, Marc Cassotti, Matteo Nikolov, Nikolai G. Nicolotti, Orazio Andersson, Patrik L. Zang, Qingda Politi, Regina Beger, Richard D. Todeschini, Roberto Huang, Ruili Farag, Sherif Rosenberg, Sine A. Slavov, Svetoslav Hu, Xin Judson, Richard S. TI CERAPP: Collaborative Estrogen Receptor Activity Prediction Project SO ENVIRONMENTAL HEALTH PERSPECTIVES LA English DT Article ID ENDOCRINE-DISRUPTING CHEMICALS; COMPUTATIONAL TOXICOLOGY RESOURCE; QUANTITATIVE STRUCTURE-ACTIVITY; THROUGHPUT SCREENING ASSAYS; RELATIONSHIP QSDAR MODELS; ENVIRONMENTAL CHEMICALS; DECISION FOREST; PARTITION-COEFFICIENTS; BINARY CLASSIFICATION; BIOLOGICAL-ACTIVITY AB BACKGROUND: Humans are exposed to thousands of man-made chemicals in the environment. Some chemicals mimic natural endocrine hormones and, thus, have the potential to be endocrine disruptors. Most of these chemicals have never been tested for their ability to interact with the estrogen receptor (ER). Risk assessors need tools to prioritize chemicals for evaluation in costly in vivo tests, for instance, within the U.S. EPA Endocrine Disruptor Screening Program. OBJECTIVES: We describe a large-scale modeling project called CERAPP (Collaborative Estrogen Receptor Activity Prediction Project) and demonstrate the efficacy of using predictive computational models trained on high-throughput screening data to evaluate thousands of chemicals for ER-related activity and prioritize them for further testing. METHODS: CERAPP combined multiple models developed in collaboration with 17 groups in the United States and Europe to predict ER activity of a common set of 32,464 chemical structures. Quantitative structure-activity relationship models and docking approaches were employed, mostly using a common training set of 1,677 chemical structures provided by the U.S. EPA, to build a total of 40 categorical and 8 continuous models for binding, agonist, and antagonist ER activity. All predictions were evaluated on a set of 7,522 chemicals curated from the literature. To overcome the limitations of single models, a consensus was built by weighting models on scores based on their evaluated accuracies. RESULTS: Individual model scores ranged from 0.69 to 0.85, showing high prediction reliabilities. Out of the 32,464 chemicals, the consensus model predicted 4,001 chemicals (12.3%) as high priority actives and 6,742 potential actives (20.8%) to be considered for further testing. CONCLUSION: This project demonstrated the possibility to screen large libraries of chemicals using a consensus of different in silico approaches. This concept will be applied in future projects related to other end points. C1 [Mansouri, Kamel; Richard, Ann M.; Grulke, Christopher M.; Kancherla, Jayaram; Judson, Richard S.] US EPA, Natl Ctr Computat Toxicol, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. [Mansouri, Kamel] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. [Abdelaziz, Ahmed; Tetko, Igor V.] German Res Ctr Environm Hlth GmbH, Helmholtz Zentrum Muenchen, Inst Struct Biol, Neuherberg, Germany. [Rybacka, Aleksandra; Andersson, Patrik L.] Umea Univ, Dept Chem, Umea, Sweden. [Roncaglioni, Alessandra; Benfenati, Emilio; Incisivo, Giuseppina M.] Ist Ric Farmacol Mario Negri, IRCCS, Environm Chem & Toxicol Lab, Milan, Italy. [Tropsha, Alexander; Fourches, Denis; Muratov, Eugene; Politi, Regina; Farag, Sherif] Univ North Carolina Chapel Hill, Lab Mol Modeling, Chapel Hill, NC USA. [Varnek, Alexandre; Horvath, Dragos] Univ Strasbourg, Lab Chemoinformat, Strasbourg, France. [Zakharov, Alexey; Nicklaus, Marc] NCI, NIH, US Dept HHS, Bethesda, MD 20892 USA. [Worth, Andrew; Burton, Julien] European Commiss Ispra, Joint Res Ctr, IHCP, Ispra, Italy. [Trisciuzzi, Daniela; Mangiatordi, Giuseppe F.; Nicolotti, Orazio] Univ Bari, Dept Pharmacy Drug Sci, Bari, Italy. [Wedebye, Eva Bay; Nikolov, Nikolai G.; Rosenberg, Sine A.] Tech Univ Denmark, Natl Food Inst, Div Toxicol & Risk Assessment, Copenhagen, Denmark. [Grisoni, Francesca; Cassotti, Matteo; Todeschini, Roberto] Univ Milano Bicocca, Milano Chemometr & QSAR Res Grp, Milan, Italy. [Hong, Huixiao; Ng, Hui W.] US FDA, Div Bioinformat & Biostat, Natl Ctr Toxicol Res, USDA, Jefferson, AZ USA. [Tetko, Igor V.] BigChem GmbH, Neuherberg, Germany. [Balabin, Ilya] Lockheed Martin, High Performance Comp, Res Triangle Pk, NC USA. [Shen, Jie] Res Inst Fragrance Mat Inc, Woodcliff Lake, NJ USA. [Zang, Qingda; Slavov, Svetoslav] Integrated Lab Syst Inc, Res Triangle Pk, NC USA. [Beger, Richard D.] USDA, Div Syst Biol, Natl Ctr Toxicol Res, Jefferson, AZ USA. [Huang, Ruili; Hu, Xin] NIH, Natl Ctr Adv Translat Sci, DHHS, Bldg 10, Bethesda, MD 20892 USA. RP Judson, RS (reprint author), US EPA, Natl Ctr Computat Toxicol, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. EM judson.richard@epa.gov RI Tetko, Igor/B-1540-2010; Varnek, Alexandre/E-7076-2017; Kancherla, Jayaram/P-7756-2015; OI Tetko, Igor/0000-0002-6855-0012; Varnek, Alexandre/0000-0003-1886-925X; Kancherla, Jayaram/0000-0001-5855-5031; Mansouri, Kamel/0000-0002-6426-8036 FU NIGMS NIH HHS [T32 GM067553] NR 101 TC 14 Z9 14 U1 14 U2 14 PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE PI RES TRIANGLE PK PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233, RES TRIANGLE PK, NC 27709-2233 USA SN 0091-6765 EI 1552-9924 J9 ENVIRON HEALTH PERSP JI Environ. Health Perspect. PD JUL PY 2016 VL 124 IS 7 BP 1023 EP 1033 DI 10.1289/ehp.1510267 PG 11 WC Environmental Sciences; Public, Environmental & Occupational Health; Toxicology SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology GA DS4KF UT WOS:000380749300025 PM 26908244 ER PT J AU Thompson, D Cullen, KW Redondo, MJ Anderson, B AF Thompson, Debbe Cullen, Karen W. Redondo, Maria J. Anderson, Barbara TI Use of Relational Agents to Improve Family Communication in Type 1 Diabetes: Methods SO JMIR RESEARCH PROTOCOLS LA English DT Article DE adolescents; family communication; pre-adolescents; relational agent; type 1 diabetes ID GLYCEMIC CONTROL; PHYSICAL-ACTIVITY; CLINICAL-RESEARCH; METABOLIC-CONTROL; ADHERENCE; YOUTH; MANAGEMENT; CONFLICT; ADOLESCENTS; DESIGN AB Background: Physiological and environmental risk factors interact to undermine blood glucose control during early adolescence. This has been documented to be associated with family conflict and poor adherence to diabetes management tasks. Family Teamwork is an efficacious program demonstrated to enhance family communication and reduce conflict during this vulnerable period. It was designed to be delivered to families in-person, which limited reach and potential impact. Objective: The purpose of this paper is to present the protocol for adapting Family Teamwork for Web-based delivery. Methods: Formative research with health care providers, parents, and adolescents will help modify Family Teamwork for Web-based delivery by a relational agent (ie, a computerized character with human-like features and actions). Sessions will be interactive, requiring both parent and adolescent participation, with the relational agent serving as a health coach. After programming, usability testing will be conducted to help ensure the program is easy to use. Video and instructional materials will be developed to facilitate use, and a small pilot study will be conducted to assess feasibility. Families will provide written informed consent prior to participation in any phase of the study. The Institutional Review Board at Baylor College of Medicine reviewed and approved the protocol (H-37245). Results: Formative research is underway. No results are available at this time. Conclusions: This research has the potential to make an important contribution to diabetes management by using technology to enhance the reach of an efficacious program. C1 [Thompson, Debbe; Cullen, Karen W.] Baylor Coll Med, Dept Pediat, USDA, ARS,Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. [Redondo, Maria J.] Baylor Coll Med, Pediat Endocrinol & Metab, Texas Childrens Hosp, Houston, TX USA. [Anderson, Barbara] Baylor Coll Med, Dept Pediat, Psychol, Houston, TX 77030 USA. RP Thompson, D (reprint author), Baylor Coll Med, Dept Pediat, USDA, ARS,Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM dit@bcm.edu OI Redondo, Maria/0000-0001-5871-4645; CULLEN, KAREN/0000-0002-5114-6232; Thompson, Debbe/0000-0002-5491-8816 FU NIDDK NIH HHS [R21 DK106505] NR 53 TC 0 Z9 0 U1 6 U2 6 PU JMIR PUBLICATIONS, INC PI TORONTO PA 59 WINNERS CIRCLE, TORONTO, ON M4L 3Y7, CANADA SN 1929-0748 J9 JMIR RES PROTOC JI JMIR RES. Protoc. PD JUL-SEP PY 2016 VL 5 IS 3 BP 118 EP 127 AR e151 DI 10.2196/resprot.5817 PG 10 WC Health Care Sciences & Services SC Health Care Sciences & Services GA DT1AR UT WOS:000381214600012 PM 27468762 ER PT J AU Kumar, I Swaminathan, K Hudson, K Hudson, ME AF Kumar, Indrajit Swaminathan, Kankshita Hudson, Karen Hudson, Matthew E. TI Evolutionary divergence of phytochrome protein function in Zea mays PIF3 signaling SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Arabidopsis; bHLH; PIF; maize; phyB; phytochrome; subfunctionalization ID TRANSCRIPTION FACTOR FAMILY; LIGHT SIGNALS; ARABIDOPSIS-THALIANA; INTERACTING FACTOR; GENE FAMILY; EXPRESSION; MAIZE; RICE; BIOSYNTHESIS; REGULATOR AB phyB and PIF3 share a common protein-protein signaling mechanism in maize and Arabidopsis. However, phyB1 and phyB2 in maize do not share this mechanism, despite being closely related, indicating recent evolutionary divergence.Two maize phytochrome-interacting factor (PIF) basic helix-loop-helix (bHLH) family members, ZmPIF3.1 and ZmPIF3.2, were identified, cloned and expressed in vitro to investigate light-signaling interactions. A phylogenetic analysis of sequences of the maize bHLH transcription factor gene family revealed the extent of the PIF family, and a total of seven predicted PIF-encoding genes were identified from genes encoding bHLH family VIIa/b proteins in the maize genome. To investigate the role of maize PIFs in phytochrome signaling, full-length cDNAs for phytochromes PhyA2, PhyB1, PhyB2 and PhyC1 from maize were cloned and expressed in vitro as chromophorylated holophytochromes. We showed that ZmPIF3.1 and ZmPIF3.2 interact specifically with the Pfr form of maize holophytochrome B1 (ZmphyB1), showing no detectable affinity for the Pr form. Maize holophytochrome B2 (ZmphyB2) showed no detectable binding affinity for PIFs in either Pr or Pfr forms, but phyB Pfr from Arabidopsis interacted with ZmPIF3.1 similarly to ZmphyB1 Pfr. We conclude that subfunctionalization at the protein-protein interaction level has altered the role of phyB2 relative to that of phyB1 in maize. Since the phyB2 mutant shows photomorphogenic defects, we conclude that maize phyB2 is an active photoreceptor, without the binding of PIF3 seen in other phyB family proteins. C1 [Kumar, Indrajit; Swaminathan, Kankshita; Hudson, Matthew E.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Kumar, Indrajit; Hudson, Matthew E.] Univ Illinois, Physiol & Mol Plant Biol, Urbana, IL 61801 USA. [Hudson, Karen] USDA ARS, Crop Prod & Pest Control Res Unit, 915 West State St, W Lafayette, IN 47907 USA. RP Hudson, ME (reprint author), Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA.; Hudson, ME (reprint author), Univ Illinois, Physiol & Mol Plant Biol, Urbana, IL 61801 USA. EM mhudson@illinois.edu RI Hudson, Matthew/A-4438-2008 OI Hudson, Matthew/0000-0002-4737-0936 FU US Department of Agriculture under the Illinois-Missouri Biotechnology Alliance program; University of Illinois FX We thank Soyon Lee for assistance with library construction, and Gopal Battu, Ying Li, Kranthi Varala, Mohammad Belafif and Tong-Geon Lee in the laboratory for helpful discussion. This research was funded by a grant from the US Department of Agriculture to MEH under the Illinois-Missouri Biotechnology Alliance program, and by a startup grant to MEH from the University of Illinois. NR 48 TC 2 Z9 2 U1 5 U2 6 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD JUL PY 2016 VL 67 IS 14 BP 4231 EP 4240 DI 10.1093/jxb/erw217 PG 10 WC Plant Sciences SC Plant Sciences GA DU2SE UT WOS:000382060300019 PM 27262126 ER PT J AU Gonzalez-Astudillo, V Hernandez, SM Yabsley, MJ Mead, DG Keel, KM Munk, BA Fischer, JR Ruder, MG Brown, JD Peters, VE Nemeth, NM AF Gonzalez-Astudillo, Viviana Hernandez, Sonia M. Yabsley, Michael J. Mead, Daniel G. Keel, Kevin M. Munk, Brandon A. Fischer, John R. Ruder, Mark G. Brown, Justin D. Peters, Valerie E. Nemeth, Nicole M. TI MORTALITY OF SELECTED AVIAN ORDERS SUBMITTED TO A WILDLIFE DIAGNOSTIC LABORATORY (SOUTHEASTERN COOPERATIVE WILDLIFE DISEASE STUDY, USA): A 36-YEAR RETROSPECTIVE ANALYSIS SO JOURNAL OF WILDLIFE DISEASES LA English DT Review DE Data set; diagnosis; disease; infection; mortality; wild bird ID WEST-NILE-VIRUS; DEER ODOCOILEUS-VIRGINIANUS; IN-HOUSE FINCHES; UNITED-STATES; MYCOPLASMAL CONJUNCTIVITIS; MELANERPES-CAROLINUS; INFECTIOUS-DISEASE; MOLOTHRUS-ATER; AQUATIC BIRDS; PESTICIDES AB To determine the relative importance of mortality factors for birds and to assess for patterns in avian mortality over time, we retrospectively examined data of birds submitted to the Southeastern Cooperative Wildlife Disease Study (SCWDS; http://vet.uga.edu/scwds), US, from 1976 to 2012. During this period, SCWDS, a wildlife diagnostic laboratory, received 2,583 wild bird specimens, from the taxonomic orders Apodiformes, Caprimulgiformes, Cuculiformes, Passeriformes, and Piciformes, originating from 22 states. Data from 2,001 of these birds were analyzed using log-linear models to explore correlations between causes of mortality, taxonomic family, demography, geographic location, and seasonality. Toxicosis was the major cause of mortality, followed by trauma, bacterial infection, physiologic stress, viral infection, and other (mortality causes with low sample numbers and etiologies inconsistent with established categories). Birds submitted during fall and winter had a higher frequency of parasitic infections, trauma, and toxicoses, whereas birds submitted during the spring and summer were more likely to die of an infectious disease, physiologic stress, or trauma. We noted a decrease in toxicoses concurrent with an increase in bacterial infections and trauma diagnoses after the mid-1990s. Toxicosis was the most commonly diagnosed cause of death among adult birds; the majority of juveniles died from physiologic stress, trauma, or viral infections. Infectious agents were diagnosed more often within the families Cardinalidae and Fringilidae, whereas noninfectious etiologies were the primary diagnoses in the Bombycillidae, Parulidae, Sturnidae, Turdidae, and Icteridae. There are important inherent limitations in the examination of data from diagnostic labs, as submission of cases varies in timing, frequency, location, and species and is often influenced by several factors, including media coverage of high-profile mortality events. Notwithstanding, our data provide a rare opportunity to examine long-term, regional, and temporal patterns in causes of avian mortality, and they allow for the analysis of novel and rare mortality factors. C1 [Gonzalez-Astudillo, Viviana; Hernandez, Sonia M.; Yabsley, Michael J.] Univ Georgia, Warnell Sch Forestry & Nat Resources, 180 E Green St, Athens, GA 30602 USA. [Hernandez, Sonia M.; Yabsley, Michael J.; Mead, Daniel G.; Keel, Kevin M.; Munk, Brandon A.; Fischer, John R.; Ruder, Mark G.; Brown, Justin D.] Univ Georgia, Southeastern Cooperat Wildlife Dis Study, Dept Populat Hlth, Coll Vet Med, 589 DW Brooks Dr, Athens, GA 30602 USA. [Peters, Valerie E.] Miami Univ, Dept Biol, 700 E High St, Oxford, OH 45056 USA. [Peters, Valerie E.] Miami Univ, Inst Environm & Sustainabil, 700 E High St, Oxford, OH 45056 USA. [Nemeth, Nicole M.] Univ Guelph, Ontario Vet Coll, Dept Pathobiol, 50 Stone Rd E, Guelph, ON N1G 2W1, Canada. [Gonzalez-Astudillo, Viviana] Univ Queensland, Sch Vet Sci, Bldg 8114, Gatton, Qld 4343, Australia. [Keel, Kevin M.] Univ Calif Davis, Sch Vet Med, Dept Pathol Microbiol & Immunol, One Shields Ave, Davis, CA 95616 USA. [Ruder, Mark G.] ARS, Arthropod Borne Anim Dis Res Unit, USDA, 1515 Coll Ave, Manhattan, KS 66502 USA. [Brown, Justin D.] Penn State Univ, Penn Game Commiss, Anim Diagnost Lab, University Pk, PA 16802 USA. [Peters, Valerie E.] Eastern Kentucky Univ, Dept Biol Sci, 521 Lancaster Ave, Richmond, KY 40475 USA. RP Gonzalez-Astudillo, V (reprint author), Univ Georgia, Warnell Sch Forestry & Nat Resources, 180 E Green St, Athens, GA 30602 USA.; Gonzalez-Astudillo, V (reprint author), Univ Queensland, Sch Vet Sci, Bldg 8114, Gatton, Qld 4343, Australia. EM v.gonzalez@uq.edu.au NR 88 TC 0 Z9 0 U1 4 U2 4 PU WILDLIFE DISEASE ASSOC, INC PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0090-3558 EI 1943-3700 J9 J WILDLIFE DIS JI J. Wildl. Dis. PD JUL PY 2016 VL 52 IS 3 BP 441 EP 458 DI 10.7589/2015-05-117 PG 18 WC Veterinary Sciences SC Veterinary Sciences GA DT5NA UT WOS:000381528700001 PM 27187034 ER PT J AU Pope, KL Wengert, GM Foley, JE Ashton, DT Botzler, RG AF Pope, Karen L. Wengert, Greta M. Foley, Janet E. Ashton, Donald T. Botzler, Richard G. CA Ecoclub Amphibian Grp TI CITIZEN SCIENTISTS MONITOR A DEADLY FUNGUS THREATENING AMPHIBIAN COMMUNITIES IN NORTHERN COASTAL CALIFORNIA, USA SO JOURNAL OF WILDLIFE DISEASES LA English DT Article DE Amphibian disease; amphibian monitoring; Batrachochytrium dendrobatidis; Bd; chytrid fungus; Northern California; youth citizen science ID PATHOGEN BATRACHOCHYTRIUM-DENDROBATIDIS; RANA-CASCADAE; CHYTRIDIOMYCOSIS; DISEASE; FROGS; MORTALITY; DECLINES; TADPOLES AB Ecoclub youth and supervising family members conducted citizen science to assess regional prevalence and distribution of Batrachochytrium dendrobatidis (Bd) among amphibians at Humboldt Bay National Wildlife Refuge (Refuge) and Redwood National and State Parks (Parks), Humboldt County, California, US, May 2013 through December 2014. Using quantitative real-time PCR, 26 (17%) of 155 samples were positive for Bd. Positive samples occurred in four frog and toad species: foothill yellow-legged frog (Rana boylii), northern red-legged frog (Rana aurora), Pacific chorus frog (Pseudacris regilla), and western toad (Anaxyrus [Bufo] boreas); no salamanders or anuran larvae were positive. Except for R. aurora, all infected anurans were first-time species reports for coastal northern California. At the Refuge, significantly fewer (6/71) postmetamorphic amphibians were positive compared to the Parks (20/69; P = 0.0018). We assessed the association of being PCR-positive for Bd, season of sampling, and age of sampler (child, teen, or adult). The full model with season, species, and sampler age had the greatest support. Frogs tested in winter or spring were more likely to be positive than those tested in summer or fall; foothill yellow-legged frogs, northern red-legged frogs, and western toads were more likely to be positive than were Pacific chorus frogs; and the probability of being positive nearly doubled when a child (<= 12 yr old) collected the sample compared to a teen or adult. Our results support other chytrid studies that found amphibians are more susceptible to Bd when temperatures are cool and that species differ in their susceptibility. The Ecoclub's findings provide new information important to conservation of northern California's coastal amphibians and demonstrate the value of involving children in citizen science. C1 [Pope, Karen L.; Botzler, Richard G.] Bilingual McKinleyville Ecoclub, 1363 Whitmire Ave, McKinleyville, CA 95519 USA. [Ecoclub Amphibian Grp] US Forest Serv, Pacific Southwest Res Stn, Redwood Sci Lab, 1700 Bayview Dr, Arcata, CA 95521 USA. [Wengert, Greta M.] Integral Ecol Res Ctr, 431 1st Ave, Blue Lake, CA 95525 USA. [Foley, Janet E.] Univ Calif Davis, Sch Vet Med, 1320 Tupper Hall, Davis, CA 95616 USA. [Ashton, Donald T.] McBain Associates, 980 7th St, Arcata, CA 95521 USA. [Botzler, Richard G.] Humboldt State Univ, Dept Wildlife, 1 Harpst St, Arcata, CA 95521 USA. RP Botzler, RG (reprint author), Bilingual McKinleyville Ecoclub, 1363 Whitmire Ave, McKinleyville, CA 95519 USA.; Botzler, RG (reprint author), Humboldt State Univ, Dept Wildlife, 1 Harpst St, Arcata, CA 95521 USA. EM botzlerr@sbcglobal.net FU Arcata-McKinleyville Children's Centers; Friends of Humboldt Bay National Wildlife Refuge; Archie Bernardi Memorial Fund (Humboldt Area Foundation); Andree Wagner Peace Trust; Mary Susan Hansen Trust; Rev. S. J. Hill Trust; Humboldt Back & Neck Pain Center FX Permits from supporting agencies included California Scientific Collecting Permits 12564 (R.G.B.), 3905 (K.L.P.), and 0080 (D.T.A.); Humboldt State University Institutional and Care and Use Committee Permit 12/13.B.22-A; US Fish and Wildlife Service Permit 81590-12014; California Department of Parks and Recreation Permit 14-635-010; and National Park Service Permit REDW-2013-SCI-0011. We appreciate the support of many professionals including M. Davies-Hughes, E. Nelson, K. Griggs, D. Seeger, K. Bensen, J. Harris, M. Gabriel, A. Cummings, C. Wheeler, and M. Van Hattem. We are grateful for funding support from the Arcata-McKinleyville Children's Centers, Friends of Humboldt Bay National Wildlife Refuge, Archie Bernardi Memorial Fund (Humboldt Area Foundation), Andree Wagner Peace Trust, Mary Susan Hansen Trust, Rev. S. J. Hill Trust, Humboldt Back & Neck Pain Center, and personal contributions from many community members. NR 33 TC 0 Z9 0 U1 14 U2 14 PU WILDLIFE DISEASE ASSOC, INC PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0090-3558 EI 1943-3700 J9 J WILDLIFE DIS JI J. Wildl. Dis. PD JUL PY 2016 VL 52 IS 3 BP 516 EP 523 DI 10.7589/2015-10-280 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA DT5NA UT WOS:000381528700008 ER PT J AU Comeaux, JM Curtis-Robles, R Lewis, BC Cummings, KJ Mesenbrink, BT Leland, BR Bodenchuk, MJ Hamer, SA AF Comeaux, Juliette M. Curtis-Robles, Rachel Lewis, Barbara C. Cummings, Kevin J. Mesenbrink, Brian T. Leland, Bruce R. Bodenchuk, Michael J. Hamer, Sarah A. TI Survey of Feral Swine (Sus scrofa) Infection with the Agent of Chagas Disease (Trypanosoma cruzi) in Texas, 2013-14 SO JOURNAL OF WILDLIFE DISEASES LA English DT Article DE Chagas disease; feral swine; Sus scrofa; Texas; Trypanosoma cruzi ID PIGS; DOGS; USA AB Feral swine (Sus scrofa) are an invasive species and reservoir of numerous zoonotic pathogens in the US, and Texas leads the nation in the estimated population size of feral hogs. Texas also harbors enzootic transmission cycles of the protozoan parasite Trypanosoma cruzi, agent of Chagas disease. Given previous evidence that swine can serve as reservoirs of T. cruzi in Latin America and new evidence of triatomines (kissing bugs) feeding on swine in Texas, we measured the prevalence of T. cruzi infection in feral swine in Texas. From 2013 to 2014, we sampled blood and/or cardiac tissue from 78 feral swine across 14 Texas counties (seven with and seven without prior documentation of kissing bug occurrence) and used PCR and histopathology to detect T. cruzi infection. We determined an overall infection prevalence of 6% (3 of 54) based on PCR evaluation of cardiac tissue, and no blood samples were positive (n = 72). All three positive pigs were from counties where kissing bugs are documented. No T. cruzi amastigotes were noted on histopathology (n = 54). Sarcocysts were observed in 10 (18%) of the samples, five of which also had mild focal areas of degeneration and inflammatory cell infiltration. Eco-epidemiologic investigations can provide an assessment of contributions of feral hogs to maintenance of T. cruzi across a landscape to help protect human and animal health. C1 [Comeaux, Juliette M.; Curtis-Robles, Rachel; Cummings, Kevin J.; Hamer, Sarah A.] Texas A&M Univ, Coll Vet Med & Biomed Sci, 4458 TAMU, College Stn, TX 77843 USA. [Lewis, Barbara C.] Texas A&M Vet Med Diagnost Lab, PO Drawer 3040, College Stn, TX 77841 USA. [Mesenbrink, Brian T.; Leland, Bruce R.; Bodenchuk, Michael J.] Wildlife Serv, USDA, APHIS, POB 690170, San Antonio, TX 78269 USA. RP Hamer, SA (reprint author), Texas A&M Univ, Coll Vet Med & Biomed Sci, 4458 TAMU, College Stn, TX 77843 USA. EM shamer@cvm.tamu.edu FU National Science Foundation Graduate Research Fellowship Program [1252521]; Texas A&M University-Texas Veterinary Medical Diagnostic Lab Seed Grant FX We thank Lisa Auckland, Mary Grigar, Carolyn Hodo, Erica Holcomb, Alan Mai, Sarah Noe, and Lorraine Rodriguez-Rivera for assistance in the laboratory. Partial funding was provided by the National Science Foundation Graduate Research Fellowship Program (1252521 to RC-R) and the Texas A&M University-Texas Veterinary Medical Diagnostic Lab Seed Grant. NR 17 TC 0 Z9 0 U1 4 U2 4 PU WILDLIFE DISEASE ASSOC, INC PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0090-3558 EI 1943-3700 J9 J WILDLIFE DIS JI J. Wildl. Dis. PD JUL PY 2016 VL 52 IS 3 BP 627 EP 630 DI 10.7589/2015-08-208 PG 4 WC Veterinary Sciences SC Veterinary Sciences GA DT5NA UT WOS:000381528700022 PM 27224214 ER PT J AU Love, D Kwok, OC Verma, SK Dubey, JP Bellah, J AF Love, David Kwok, Oliver C. Verma, Shiv Kumar Dubey, Jitender P. Bellah, Jamie TI Antibody Prevalence and Isolation of Viable Toxoplasma gondii from Raptors in the Southeastern USA SO JOURNAL OF WILDLIFE DISEASES LA English DT Article DE Genotype; isolation; raptor; serology; Toxoplasma gondii; type II ID WILD BIRDS AB Raptors are good indicators of the prevalence of Toxoplasma gondii in the environment because they prey on small mammals and birds. These prey species are a major source of infection in domestic cats (Felis catus), which shed the environmentally resistant oocysts. We assessed T. gondii infection in 281 opportunistically available raptors at a rehabilitation facility between 2012 and 2014. Antibodies to T. gondii were assayed by a modified agglutination test (cutoff 1: 25) and found in serum of 22/71 Red-tailed Hawks (Buteo jamaicensis), 25/54 Barred Owls (Strix varia), 9/41 Red-shouldered Hawks (Buteo lineatus), 13/28 Great Horned Owls (Bubo virginianus), 6/20 Broad-winged Hawks (Buteo platypterus), 2/16 Eastern Screech Owls (Megascops asio), 12/13 Bald Eagles (Haliaeetus leucocephalus), 6/12 Cooper's Hawks (Accipiter cooperii), 1/8 Black Vultures (Coragyps atratus), and 1/1 Golden Eagle (Aquila chrysaetos). Antibodies were not detected in 5 Barn Owls (Tyto alba), 3 American Kestrels (Falco sparverius), 1 Mississippi Kite (Ictinia mississippiensis), and 1 Osprey (Pandion haliaetus). Viable T. gondii was isolated from the tissues of 1 antibody-positive Barred Owl and identified as a strain having type II alleles at all 10 loci tested, except one (ToxoDB polymerase chain reaction-restriction fragment length polymorphism genotype 3). Type II strain is the most common strain in the US. Results of this study indicate a high prevalence of T. gondii in some raptor species and the first reported genotyping from a Barred Owl. C1 [Love, David] Wilds Conservat Ctr, 14000 Int Rd, Cumberland, OH 43732 USA. [Kwok, Oliver C.; Verma, Shiv Kumar; Dubey, Jitender P.] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Bldg 1001, Beltsville, MD 20705 USA. [Bellah, Jamie] Auburn Univ, Coll Vet Med, Dept Clin Sci, 1220 Wire Rd, Auburn, AL 36849 USA. RP Love, D (reprint author), Wilds Conservat Ctr, 14000 Int Rd, Cumberland, OH 43732 USA. EM dml0011@tigermail.auburn.edu NR 11 TC 0 Z9 0 U1 9 U2 10 PU WILDLIFE DISEASE ASSOC, INC PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0090-3558 EI 1943-3700 J9 J WILDLIFE DIS JI J. Wildl. Dis. PD JUL PY 2016 VL 52 IS 3 BP 653 EP 656 DI 10.7589/2015-10-269 PG 4 WC Veterinary Sciences SC Veterinary Sciences GA DT5NA UT WOS:000381528700027 PM 27243150 ER PT J AU Pedersen, K Marks, DR Afonso, CL Stopak, SR Williams-Coplin, D Dimitrov, KM Miller, PJ DeLiberto, TJ AF Pedersen, Kerri Marks, David R. Afonso, Claudio L. Stopak, Scott R. Williams-Coplin, Dawn Dimitrov, Kiril M. Miller, Patti J. DeLiberto, Thomas J. TI Identification of Avian Paramyxovirus Serotype-1 in Wild Birds in the USA SO JOURNAL OF WILDLIFE DISEASES LA English DT Article DE Avian paramxyovirus serotype-1; Newcastle disease; Newcastle disease virus; pigeon paramyxovirus serotype-1; wild birds ID NEWCASTLE-DISEASE VIRUS; IMMUNE-RESPONSE; UNITED-STATES; POULTRY; CORMORANTS; SHOREBIRDS; INFLUENZA; VIRULENCE; WATERFOWL; CHICKENS AB In the US, sampling for avian paramyxovirus serotype-1 (APMV-1) is generally conducted when morbidity or mortality events occur involving certain families of wild birds known to be affected by the virus, such as cormorants (Family Phalacrocoracidae), pigeons, doves (Family Columbidae), or pelicans (Family Pelecanidae). To quantify the prevalence of APMV-1 in apparently healthy wild birds and to determine its geographic distribution, we collected swab and serum samples from >3,500 wild birds, representing eight orders from 1 January 2013 to 30 September 2013. Antibody prevalence was highest in wild birds of Order Suliformes (44.9%), followed by Pelecaniformes (24.4%), Anseriformes (22.7%), and Columbiformes (11.7%), with a relatively high occurrence of virulent viruses in Columbiformes (100% of virulent viruses isolated). As expected, viral shedding was comparatively much lower, and positives were only identified in Orders Accipitriformes (1.4%), Columbiformes (1.0%), Anseriformes (0.8%), and Charadriiformes (0.4%). We also demonstrate circulating virulent APMV-1 viruses of genotype VI in apparently healthy Rock Pigeons (Columba livia) from March through September in three states. C1 [Pedersen, Kerri] Wildlife Serv, USDA, Anim & Plant Hlth Inspect Serv, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. [Marks, David R.] Wildlife Serv, USDA, Anim & Plant Hlth Inspect Serv, 2803 Jolly Rd,Suite 100, Okemos, MI 48864 USA. [Afonso, Claudio L.; Williams-Coplin, Dawn; Dimitrov, Kiril M.; Miller, Patti J.] ARS, USDA, Southeast Poultry Res Lab, 934 Coll Stn Rd, Athens, GA 30605 USA. [Stopak, Scott R.] Wildlife Serv, USDA, Anim & Plant Hlth Inspect Serv, 9134 Blackeagle Dr, Boise, ID 83709 USA. [DeLiberto, Thomas J.] Wildlife Serv, USDA, Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. RP Pedersen, K (reprint author), Wildlife Serv, USDA, Anim & Plant Hlth Inspect Serv, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM Kerri.Pedersen@aphis.usda.gov NR 23 TC 1 Z9 1 U1 3 U2 3 PU WILDLIFE DISEASE ASSOC, INC PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0090-3558 EI 1943-3700 J9 J WILDLIFE DIS JI J. Wildl. Dis. PD JUL PY 2016 VL 52 IS 3 BP 657 EP 662 DI 10.7589/2015-10-278 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA DT5NA UT WOS:000381528700028 PM 27243153 ER PT J AU Meydani, SN Das, SK Pieper, CF Lewis, MR Klein, S Dixit, VD Gupta, AK Villareal, DT Bhapkar, M Huang, M Fuss, PJ Roberts, SB Holloszy, JO Fontana, L AF Meydani, Simin N. Das, Sai K. Pieper, Carl F. Lewis, Michael R. Klein, Sam Dixit, Vishwa D. Gupta, Alok K. Villareal, Dennis T. Bhapkar, Manjushri Huang, Megan Fuss, Paul J. Roberts, Susan B. Holloszy, John O. Fontana, Luigi TI Long-term moderate calorie restriction inhibits inflammation without impairing cell-mediated immunity: a randomized controlled trial in non-obese humans SO AGING-US LA English DT Article DE human; familial longevity; calorie restriction; inflammation; vaccine response; cell-mediated immunity ID PRIMARY INFLUENZA INFECTION; TUMOR-NECROSIS-FACTOR; C-REACTIVE PROTEIN; COMPREHENSIVE ASSESSMENT; INSULIN-RESISTANCE; LEUKOCYTE COUNT; REDUCING INTAKE; C57BL/6 MICE; WEIGHT-LOSS; ENERGY AB Calorie restriction (CR) inhibits inflammation and slows aging in many animal species, but in rodents housed in pathogen-free facilities, CR impairs immunity against certain pathogens. However, little is known about the effects of long-term moderate CR on immune function in humans. In this multi-center, randomized clinical trial to determine CR's effect on inflammation and cell-mediated immunity, 218 healthy non-obese adults (20-50 y), were assigned 25% CR (n=143) or an ad-libitum (AL) diet (n=75), and outcomes tested at baseline, 12, and 24 months of CR. CR induced a 10.4% weight loss over the 2-y period. Relative to AL group, CR reduced circulating inflammatory markers, including total WBC and lymphocyte counts, ICAM-1 and leptin. Serum CRP and TNF-alpha concentrations were about 40% and 50% lower in CR group, respectively. CR had no effect on the delayed-type hypersensitivity skin response or antibody response to vaccines, nor did it cause difference in clinically significant infections. In conclusion, long-term moderate CR without malnutrition induces a significant and persistent inhibition of inflammation without impairing key in vivo indicators of cell-mediated immunity. Given the established role of these pro-inflammatory molecules in the pathogenesis of multiple chronic diseases, these CR-induced adaptations suggest a shift toward a healthy phenotype. C1 [Meydani, Simin N.; Das, Sai K.; Fuss, Paul J.; Roberts, Susan B.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Pieper, Carl F.; Bhapkar, Manjushri; Huang, Megan] Duke Univ, Med Ctr, Durham, NC 27705 USA. [Lewis, Michael R.] Univ Vermont, Burlington, VT 05405 USA. [Klein, Sam; Holloszy, John O.; Fontana, Luigi] Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA. [Dixit, Vishwa D.] Yale Univ, Sch Med, Comparat Med & Dept Immunobiol, 333 Cedar St, New Haven, CT 06510 USA. [Gupta, Alok K.] Pennington Biomed Res Ctr, 6400 Perkins Rd, Baton Rouge, LA 70808 USA. [Villareal, Dennis T.] Baylor Coll Med, Houston, TX 77030 USA. [Villareal, Dennis T.] Michael E DeBakey VA Med Ctr, Houston, TX 77030 USA. [Fontana, Luigi] Univ Brescia, Sch Med, Dept Clin & Expt Sci, Brescia, Italy. [Fontana, Luigi] CEINGE Biotecnol Avanzate, Naples, Italy. RP Meydani, SN (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM Simin.meydani@tufts.edu FU The National Institute on Aging, National Institutes of Health Cooperative Agreements [U01AG022132, U01AG020478, U01AG020487, U01AG020480]; USDA Specific Cooperative Agreement [58-1950-0-014] FX The National Institute on Aging, National Institutes of Health Cooperative Agreements (U01AG022132, U01AG020478, U01AG020487, and U01AG020480); USDA Specific Cooperative Agreement #58-1950-0-014. NR 51 TC 2 Z9 2 U1 3 U2 4 PU IMPACT JOURNALS LLC PI ALBANY PA 6211 TIPTON HOUSE, STE 6, ALBANY, NY 12203 USA SN 1945-4589 J9 AGING-US JI Aging-US PD JUL PY 2016 VL 8 IS 7 BP 1416 EP 1431 DI 10.18632/aging.100994 PG 16 WC Cell Biology SC Cell Biology GA DU1DV UT WOS:000381949300012 PM 27410480 ER PT J AU Zhou, MC Eberhardt, TL Xin, PP Hse, CY Pan, H AF Zhou, Mengchao Eberhardt, Thomas L. Xin, Pingping Hse, Chung-Yun Pan, Hui TI Liquefaction of Torrefied Wood using Microwave Irradiation SO ENERGY & FUELS LA English DT Article ID LIGNOCELLULOSIC BIOMASS; POLYURETHANE FOAMS; TORREFACTION; PYROLYSIS; TEMPERATURE; CELLULOSE; GASIFICATION; PRETREATMENT; PERFORMANCE; REACTIVITY AB Torrefaction is an effective pretreatment method to improve the uniformity and quality of lignocellolosic biomass before further thermal processing (e.g., gasification, combustion). The objective of this study was to determine the impacts of torrefaction as a pretreatment before liquefaction. Wood chips were torrefied for 2 h at three different temperatures (230, 260, 290 degrees C) and then subjected to microwave-assisted liquefaction, as was the untreated wood control. The dielectric properties of liquefaction materials, including the biomass samples and liquefaction reagent, were measured to evaluate their abilities to convert electromagnetic energy to heat. The effects of liquefaction time, temperature, and catalyst concentration on the liquefaction efficiency were also investigated. It showed that torrefaction temperature had significant influence on the liquefaction behavior of wood materials. Wood treated at the lowest torrefaction temperature (230 degrees C) retained the most structural/compositional characteristics of untreated wood and therefore they both exhibited similar liquefaction behaviors. The higher treatment temperature (290 degrees C) led to higher liquefaction residue contents, attributed to the increase in carbon content and hydrophobicity from torrefaction. C1 [Zhou, Mengchao; Xin, Pingping; Pan, Hui] Nanjing Forestry Univ, Coll Chem Engn, 159 Longpan Rd, Nanjing 210037, Jiangsu, Peoples R China. [Eberhardt, Thomas L.] Forest Prod Lab, Madison, WI 53726 USA. [Hse, Chung-Yun] Southern Res Stn, Pineville, LA 71360 USA. RP Pan, H (reprint author), Nanjing Forestry Univ, Coll Chem Engn, 159 Longpan Rd, Nanjing 210037, Jiangsu, Peoples R China. EM hpan@njfu.edu.cn FU Forestry Industry Research Special Funds for Public Welfare Projects of China [201504602]; Jiangsu Specially-Appointed Professor program of the State Minister of Education of Jiangsu Province, China FX The authors are grateful for the financial support by Forestry Industry Research Special Funds for Public Welfare Projects of China (201504602) and the Jiangsu Specially-Appointed Professor program of the State Minister of Education of Jiangsu Province, China. NR 52 TC 2 Z9 2 U1 12 U2 14 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 EI 1520-5029 J9 ENERG FUEL JI Energy Fuels PD JUL PY 2016 VL 30 IS 7 BP 5862 EP 5869 DI 10.1021/acs.energyfuels.6b01004 PG 8 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA DS2BO UT WOS:000380523200074 ER PT J AU Li, NX Alfiky, A Vaughan, MM Kang, S AF Li, Ningxiao Alfiky, Alsayed Vaughan, Martha M. Kang, Seogchan TI Stop and smell the fungi: Fungal volatile metabolites are overlooked signals involved in fungal interaction with plants SO FUNGAL BIOLOGY REVIEWS LA English DT Review DE Chemical ecology; Inter-organismal signals; Plant fitness; Plant-fungal interaction; Semio-compounds; Volatilome ID INDUCED SYSTEMIC RESISTANCE; PSEUDOMONAS-CHLORORAPHIS O6; WOOD DECAY FUNGI; ORGANIC-COMPOUNDS; ARABIDOPSIS-THALIANA; BACTERIAL VOLATILES; FUSARIUM-OXYSPORUM; MICROBE INTERACTIONS; TRICHODERMA-VIRENS; BIOMASS PRODUCTION AB Diverse fungi are intimately associated with plants, and molecules secreted from both the plant and fungal sides play critical roles in the establishment of their associations and affect plant growth and health. Through evolutionary arms races or strategic alliances with plants, fungi and other microbes have invented a bewildering array of secreted molecules to parasitize or communicate with plants. Research empowered by omics data and tools has greatly advanced understanding of the nature, role and mechanism of action of many secreted fungal proteins that affect plants directly or indirectly. However, available information about fungal volatile metabolites with similar functions is quite limited. Through this review, we aim to stimulate expeditions to this vastly under-explored frontier of fungal chemical ecology. (C) 2016 British Mycological Society. Published by Elsevier Ltd. All rights reserved. C1 [Li, Ningxiao; Kang, Seogchan] Penn State Univ, Intercoll Grad Degree Program Plant Biol, University Pk, PA 16802 USA. [Alfiky, Alsayed; Kang, Seogchan] Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. [Alfiky, Alsayed] Tanta Univ, Fac Agr, Dept Genet, Tanta, Egypt. [Vaughan, Martha M.] ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. RP Kang, S (reprint author), Penn State Univ, Buckhout Lab 311, University Pk, PA 16802 USA. EM sxk55@psu.edu FU USDA-NIFA Agriculture and Food Research Initiative Competitive Grants Program [2010-65110-20488]; Storkan-Hanes-McCaslin Foundation; Penn State College of Agricultural Sciences; Huck Institutes of the Life Sciences; Penn State Center for Environment geoChemistry and Genomics; Fulbright Program FX Li has been supported by a training grant from the USDA-NIFA Agriculture and Food Research Initiative Competitive Grants Program (award # 2010-65110-20488) and an award from the Storkan-Hanes-McCaslin Foundation. Our research on fungal VOCs has been supported by the Penn State College of Agricultural Sciences (Jeanne & Charles Rider Endowment Award and Strategic Collaboration Seed Grant), the Huck Institutes of the Life Sciences, and the Penn State Center for Environment geoChemistry and Genomics. We would like to thank the Fulbright Program for supporting Alfiky's work at Penn State. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture (USDA). USDA is an equal opportunity provider and employer. NR 115 TC 1 Z9 1 U1 23 U2 31 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1749-4613 EI 1878-0253 J9 FUNGAL BIOL REV JI Fungal Biol. Rev. PD JUL PY 2016 VL 30 IS 3 BP 134 EP 144 DI 10.1016/j.fbr.2016.06.004 PG 11 WC Mycology SC Mycology GA DT2OY UT WOS:000381321500004 ER PT J AU Gallagher, D Pouillot, R Hoelzer, K Tang, J Dennis, SB Kause, JR AF Gallagher, Daniel Pouillot, Regis Hoelzer, Karin Tang, Jia Dennis, Sherri B. Kause, Janell R. TI Listeria monocytogenes in Retail Delicatessens: An Interagency Risk Assessment-Risk Mitigations SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Listeria monocytogenes; Retail; Risk assessment; Scenario analysis; Virtual retail deli ID COLD-SMOKED SALMON; TO-EAT FOODS; CROSS-CONTAMINATION; ASSESSMENT-MODEL; DELI MEATS; ESTABLISHMENTS; QUANTIFICATION; ENVIRONMENT; PREVALENCE; DIVERSITY AB Cross-contamination, improper holding temperatures, and insufficient sanitary practices are known retail practices that may lead to product contamination and growth of Listeria monocytogenes. However, the relative importance of control options to mitigate the risk of invasive listeriosis from ready-to-eat (RTE) products sliced or prepared at retail is not well understood. This study illustrates the utility of a quantitative risk assessment model described in a first article of this series (Pouillot, R., D. Gallagher, J. Tang, K. Hoelzer, J. Kause, and S. B. Dennis, J. Food Prot. 78: 134-145, 2015) to evaluate the public health impact associated with changes in retail deli practices and interventions. Twenty-two mitigation scenarios were modeled and evaluated under six different baseline conditions. These scenarios were related to sanitation, worker behavior, use of growth inhibitors, cross-contamination, storage temperature control, and reduction of the level of L. monocytogenes on incoming RTE food products. The mean risk per serving of RTE products obtained under these scenarios was then compared with the risk estimated in the baseline condition. Some risk mitigations had a consistent impact on the predicted listeriosis risk in all baseline conditions (e.g. presence or absence of growth inhibitor), whereas others were greatly dependent on the initial baseline conditions or practices in the deli (e.g. preslicing of products). Overall, the control of the bacterial growth and the control of contamination at its source were major factors of listeriosis risk in these settings. Although control of cross-contamination and continued sanitation were also important, the decrease in the predicted risk was not amenable to a simple solution. Findings from these predictive scenario analyses are intended to encourage improvements to retail food safety practices and mitigation strategies to control L. monocytogenes in RTE foods more effectively and to demonstrate the utility of quantitative risk assessment models to inform risk management decisions. C1 [Gallagher, Daniel; Tang, Jia] Virginia Polytech Inst & State Univ, Dept Civil & Environm Engn, 409 Durham Hall, Blacksburg, VA 24061 USA. [Pouillot, Regis; Hoelzer, Karin; Dennis, Sherri B.] US FDA, Ctr Food Safety & Appl Nutr, 5100 Paint Branch Pkwy, College Pk, MD 20740 USA. [Kause, Janell R.] US Food Safety & Inspect Serv, Off Publ Hlth Sci, USDA, 1400 Independence Ave SW, Washington, DC 20250 USA. [Hoelzer, Karin] Pew Charitable Trusts, 901 E St NW, Washington, DC 20004 USA. RP Pouillot, R (reprint author), US FDA, Ctr Food Safety & Appl Nutr, 5100 Paint Branch Pkwy, College Pk, MD 20740 USA. EM regis.pouillot@fda.hhs.gov RI Pouillot, Regis/E-8103-2010 OI Pouillot, Regis/0000-0002-6107-5212 FU Virginia Tech; USDA-FSIS [AG-3A94-P-08-0166] FX Support for this work was provided by Virginia Tech in collaboration with the USDA-FSIS (contract AG-3A94-P-08-0166). This work was also supported by appointments to the Research Participation Program at the Center for Food Safety and Applied Nutrition administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and the USDA. NR 35 TC 1 Z9 1 U1 6 U2 7 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUL PY 2016 VL 79 IS 7 BP 1076 EP 1088 DI 10.4315/0362-028X.JFP-15-336 PG 13 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DU0PU UT WOS:000381907800001 PM 27357026 ER PT J AU Hildebrandt, IM Marks, BP Juneja, VK Osoria, M Hall, NO Ryser, ET AF Hildebrandt, Ian M. Marks, Bradley P. Juneja, Vijay K. Osoria, Marangeli Hall, Nicole O. Ryser, Elliot T. TI Cross-Laboratory Comparative Study of the Impact of Experimental and Regression Methodologies on Salmonella Thermal Inactivation Parameters in Ground Beef SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Bacteria; Error; Meat; Modeling; Thermal resistance; Uncertainty ID ESCHERICHIA-COLI O157-H7; NONLINEAR SURVIVAL CURVES; HEAT-RESISTANCE; LISTERIA-MONOCYTOGENES; INJURED SALMONELLA; WATER ACTIVITY; TYPHIMURIUM DT104; SODIUM LACTATE; TEMPERATURE; CHICKEN AB Isothermal inactivation studies are commonly used to quantify thermal inactivation kinetics of bacteria. Meta-analyses and comparisons utilizing results from multiple sources have revealed large variations in reported thermal resistance parameters for Salmonella, even when in similar food materials. Different laboratory or regression methodologies likely are the source of methodology-specific artifacts influencing the estimated parameters; however, such effects have not been quantified. The objective of this study was to evaluate the effects of laboratory and regression methodologies on thermal inactivation data generation, interpretation, modeling, and inherent error, based on data generated in two independent laboratories. The overall experimental design consisted of a cross-laboratory comparison using two independent laboratories (Michigan State University and U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center [ERRC] laboratories), both conducting isothermal Salmonella inactivation studies (55, 60, 62 degrees C) in ground beef, and each using two methodologies reported in prior studies. Two primary models (log-linear and Weibull) with one secondary model (Bigelow) were fitted to the resultant data using three regression methodologies (two two-step regressions and a one-step regression). Results indicated that laboratory methodology impacted the estimated D60 degrees C- and z-values (alpha = 0.05), with the ERRC methodology yielding parameter estimates similar to 25% larger than the Michigan State University methodology, regardless of the laboratory. Regression methodology also impacted the model and parameter error estimates. Two-step regressions yielded root mean square error values on average 40% larger than the one-step regressions. The Akaike Information Criterion indicated the Weibull as the more correct model in most cases; however, caution should be used to confirm model robustness in application to real-world data. Overall, the results suggested that laboratory and regression methodologies have a large influence on resultant data and the subsequent estimation of thermal resistance parameters. C1 [Hildebrandt, Ian M.; Marks, Bradley P.; Hall, Nicole O.] Michigan State Univ, Dept Biosyst & Agr Engn, 524 South Shaw Lane, E Lansing, MI 48824 USA. [Ryser, Elliot T.] Michigan State Univ, Dept Food Sci & Human Nutr, 524 South Shaw Lane, E Lansing, MI 48824 USA. [Juneja, Vijay K.; Osoria, Marangeli] ARS, Eastern Reg Res Ctr, USDA, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. RP Marks, BP (reprint author), Michigan State Univ, Dept Biosyst & Agr Engn, 524 South Shaw Lane, E Lansing, MI 48824 USA. EM marksbp@msu.edu FU (National Institute of Food and Agriculture), U.S. Department of Agriculture [2007-51110-18247] FX This material is based upon work supported by the Cooperative State Research, Education, and Extension Service (now the National Institute of Food and Agriculture), U.S. Department of Agriculture, under award no. 2007-51110-18247. NR 46 TC 0 Z9 0 U1 6 U2 6 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUL PY 2016 VL 79 IS 7 BP 1097 EP 1106 DI 10.4315/0362-028X.JFP-15-496 PG 10 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DU0PU UT WOS:000381907800003 PM 27357028 ER PT J AU Olafson, PU Brown, TR Lohmeyer, KH Harvey, RB Nisbet, DJ Loneragan, GH Edrington, TS AF Olafson, Pia Untalan Brown, Tyson R. Lohmeyer, Kimberly H. Harvey, Roger B. Nisbet, David J. Loneragan, Guy H. Edrington, Thomas S. TI Assessing Transmission of Salmonella to Bovine Peripheral Lymph Nodes upon Horn Fly Feeding SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Blood feeding; Cattle; Haematobia; Salmonella ID GROUND-BEEF; HAEMATOBIA-IRRITANS; FEEDLOT CATTLE; UNITED-STATES; HIGH-PLAINS; ENTERICA; PREVALENCE; MUSCIDAE; DIPTERA; FLIES AB Biting arthropods are implicated in the transdermal transmission of Salmonella to bovine peripheral lymph nodes, and such contamination can contribute to increased Salmonella prevalence in processed beef. Since horn flies can acquire Salmonella and then excrete the bacteria in their feces, on-animal fly infestations were conducted in this study to assess whether horn flies have a role in this bacterial transmission. Three Salmonella serotypes were used to assess fly acquisition from and excretion onto cattle. The results indicated that flies can acquire Salmonella from the hide, as assessed by recovery from homogenates of surface-sterilized flies, and that Salmonella persists for at least 5 days in the fly. Fly fecal excreta serves as a bacterial contaminant on the hide, and the overall mean probable estimate of the quantity shed was approximate to 10(5) most probable number per fly cage area. In 5 days, no transmission of the bacteria to bovine peripheral lymph nodes was evident, prompting an assessment of the effects of prolonged horn fly feeding on transmission. Three groups of animals were infested with flies that had consumed a blood meal containing Salmonella Senftenberg. After 5 days, the study was either terminated or the flies were removed and the cages replenished with unfed flies either once or twice over the course of an 11- or 19-day fly exposure period, respectively. A microlancet-inoculated positive-control animal was included in each group for comparison. The impact of prolonged horn fly feeding was evident, as 8% of lymph nodes cultured were positive from the 5-day exposure, whereas 50 and 42% were positive from 11- and 19-day exposures, respectively. Higher concentrations of Salmonella were recovered from fly-infested animals than from the microlancet-inoculated control, likely a result of repeated inoculations over time by flies versus a single introduction. The data described provide new insights into the transmission dynamics of Salmonella in cattle populations, highlighting a role for biting flies as an important reservoir. C1 [Olafson, Pia Untalan; Lohmeyer, Kimberly H.] ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. [Brown, Tyson R.; Harvey, Roger B.; Nisbet, David J.; Edrington, Thomas S.] ARS, USDA, Southern Plains Agr Res Ctr, Food & Feed Safety Res, 2881 F&B Rd, College Stn, TX 77845 USA. [Loneragan, Guy H.] Texas Tech Univ, Coll Agr Sci & Nat Resources, Dept Anim & Food Sci, Int Ctr Food Ind Excellence, Lubbock, TX 79409 USA. RP Olafson, PU (reprint author), ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. EM pia.olafson@ars.usda.gov FU beef and veal producers and importers; state beef councils by the National Cattlemen's Beef Association FX The authors are grateful for the excellent technical support of Greta Buckmeier, Matthew Waldon, and Melinda May of the KBUSLIRL and Kate Andrews of the FFSRU, as well as support from animal caretaker personnel Larry Camarillo and Evelio Gonzalez of the KBUSLIRL. We are thankful to Sonia Nunez and Brandon Smythe of New Mexico State University for providing ample quantities of fly puparia from their colonized horn fly strain. This project was funded by beef and veal producers and importers through their $1-per-head checkoff and was produced for the Cattlemen's Beef Board and state beef councils by the National Cattlemen's Beef Association. This article reports the result of research only. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 23 TC 1 Z9 1 U1 2 U2 2 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUL PY 2016 VL 79 IS 7 BP 1135 EP 1142 DI 10.4315/0362-028X.JFP-15-414 PG 8 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DU0PU UT WOS:000381907800007 PM 27357032 ER PT J AU Valenzuela-Melendres, M Pena-Ramos, EA Juneja, VK Camou, JP Cumplido-Barbeitia, G AF Valenzuela-Melendres, Martin Aida Pena-Ramos, E. Juneja, Vijay K. Pedro Camou, Juan Cumplido-Barbeitia, German TI Effect of Grapefruit Seed Extract on Thermal Inactivation of Listeria monocytogenes during Sous-Vide Processing of Two Marinated Mexican Meat Entrees SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Beef; Grapefruit seed extract; Listeria monocytogenes; Meat; Pork; Sous vide; Thermal inactivation ID ESCHERICHIA-COLI O157-H7; CLOSTRIDIUM-PERFRINGENS; SALMONELLA SEROTYPES; SODIUM PYROPHOSPHATE; GROWTH-CONDITIONS; PREDICTIVE MODEL; HEAT-RESISTANCE; SPORE INOCULA; BEEF PATTIES; PRODUCTS AB D- and z-values for Listeria monocytogenes were obtained for two Mexican meat entrees: pork meat marinated in tomatillo (green tomato) sauce (PTS) and beef marinated in a red chili sauce (BRCS), with addition of 0, 200, and 800 ppm of grapefruit seed extract (GSE). Meat samples inoculated with L. monocytogenes were packaged in sterile bags, immersed in a water bath, and held at 55, 57.5, 60, and 62.5 degrees C for different periods of time. Depending upon the temperature, D-values at 0 ppm of GSE ranged from 26.19 to 2.03 min in BRCS and 26.41 to 0.8 min in PTS. Adding 800 ppm of GSE to BRCS thermally treated at 55 and 62.5 degrees C significantly decreased inactivation time by 35%. A reduction in time of 25.9, 10.6, and 40.1% at 55, 57.5, and 60 degrees C, respectively, was observed in PTS with 800 ppm of GSE. The z-values of L. monocytogenes were not significantly affected by GSE addition; average z-values were 7.25 and 5.09 degrees C for BRCS and PTS, respectively. Estimated thermal lethality for a 7-D log reduction of L. monocytogenes under commercial-size sous-vide conditions at a reference temperature of 55 degrees C was reached at 78 and 71 min for BRCS without and with 800 ppm of GSE, respectively. For PTS, 7-D reduction was attained at 69 and 61 min without and with addition of 800 ppm of GSE, respectively. Supplementing both Mexican meat entrees (BRCS and PTS) with 800 ppm of GSE rendered L. monocytogenes cells more sensitive to the lethal effect of heat. The results of this study will assist the retail food industry in designing acceptance limits on critical control points pertaining to cooking regimes to effectively eliminate L. monocytogenes in BRCS and PTS sous-vide processed Mexican meat entrees. C1 [Valenzuela-Melendres, Martin; Aida Pena-Ramos, E.; Pedro Camou, Juan; Cumplido-Barbeitia, German] Ctr Invest Alimentac & Desarrollo AC, Lab Ciencia & Tecnol Carne, Carretera Victoria, Hermosillo 83304, Sonora, Mexico. [Juneja, Vijay K.] ARS, USDA, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19308 USA. RP Pena-Ramos, EA (reprint author), Ctr Invest Alimentac & Desarrollo AC, Lab Ciencia & Tecnol Carne, Carretera Victoria, Hermosillo 83304, Sonora, Mexico. EM aida@ciad.mx NR 30 TC 0 Z9 0 U1 14 U2 15 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUL PY 2016 VL 79 IS 7 BP 1174 EP 1180 DI 10.4315/0362-028X.JFP-15-352 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DU0PU UT WOS:000381907800012 PM 27357037 ER PT J AU Guo, M Mishra, A Buchanan, RL Dubey, JP Hill, DE Gamble, HR Pradhan, AK AF Guo, Miao Mishra, Abhinav Buchanan, Robert L. Dubey, Jitender P. Hill, Dolores E. Gamble, H. Ray Pradhan, Abani K. TI Quantifying the Risk of Human Toxoplasma gondii Infection Due to Consumption of Domestically Produced Lamb in the United States SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Lamb; Meat; Risk assessment; Toxoplasma gondii ID SHEEP; PREVALENCE; ANIMALS AB Toxoplasma gondii is a prevalent protozoan parasite worldwide. Human toxoplasmosis is responsible for considerable morbidity and mortality in the United States, and meat products have been identified as an important source of T. gondii infections in humans. The goal of this study was to develop a farm-to-table quantitative microbial risk assessment model to predict the public health burden in the United States associated with consumption of U. S. domestically produced lamb. T. gondii prevalence in market lambs was pooled from the 2011 National Animal Health Monitoring System survey, and the concentration of the infectious life stage (bradyzoites) was calculated in the developed model. A log-linear regression and an exponential dose-response model were used to model the reduction of T. gondii during home cooking and to predict the probability of infection, respectively. The mean probability of infection per serving of lamb was estimated to be 1.5 cases per 100,000 servings, corresponding to similar to 6,300 new infections per year in the U. S. population. Based on the sensitivity analysis, we identified cooking as the most effective method to influence human health risk. This study provided a quantitative microbial risk assessment framework for T. gondii infection through consumption of lamb and quantified the infection risk and public health burden associated with lamb consumption. C1 [Guo, Miao; Mishra, Abhinav; Buchanan, Robert L.; Pradhan, Abani K.] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Buchanan, Robert L.; Pradhan, Abani K.] Univ Maryland, Ctr Food Safety & Secur Syst, College Pk, MD 20742 USA. [Dubey, Jitender P.; Hill, Dolores E.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Gamble, H. Ray] Natl Acad Sci, Washington, DC 20001 USA. RP Pradhan, AK (reprint author), Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA.; Pradhan, AK (reprint author), Univ Maryland, Ctr Food Safety & Secur Syst, College Pk, MD 20742 USA. EM akp@umd.edu OI Mishra, Abhinav/0000-0001-9214-0745 FU USDA, National Institute of Food and Agriculture's Agriculture and Food Research Initiative [2012-67005-19611] FX This work was supported through a grant from the USDA, National Institute of Food and Agriculture's Agriculture and Food Research Initiative (2012-67005-19611). The authors acknowledge Dr. Jeffrey L. Jones from the Centers for Disease Control and Prevention for helpful comments and contributions to this study. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the National Institute of Food and Agriculture, the Centers for Disease Control and Prevention, or the USDA. NR 33 TC 1 Z9 1 U1 1 U2 3 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUL PY 2016 VL 79 IS 7 BP 1181 EP 1187 DI 10.4315/0362-028X.JFP-15-591 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DU0PU UT WOS:000381907800013 PM 27357038 ER PT J AU Viator, CL Cates, SC Karns, SA Muth, MK Noyes, G AF Viator, Catherine L. Cates, Sheryl C. Karns, Shawn A. Muth, Mary K. Noyes, Gary TI Food Safety Practices in the Egg Products Industry SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Egg products; Food safety; Hazard analysis and critical control point; Survey AB We conducted a national census survey of egg product plants (n = 57) to obtain information on the technological and food safety practices of the egg products industry and to assess changes in these practices from 2004 to 2014. The questionnaire asked about operational and sanitation practices, microbiological testing practices, food safety training for employees, other food safety issues, and plant characteristics. The findings suggest that improvements were made in the industry's use of food safety technologies and practices between 2004 and 2014. The percentage of plants using advanced pasteurization technology and an integrated, computerized processing system increased by almost 30 percentage points. Over 90% of plants voluntarily use a written hazard analysis and critical control point (HACCP) plan to address food safety for at least one production step. Further, 90% of plants have management employees who are trained in a written HACCP plan. Most plants (93%) conduct voluntary microbiological testing. The percentage of plants conducting this testing on egg products before pasteurization has increased by almost 30 percentage points since 2004. The survey findings identify strengths and weaknesses in egg product plants' food safety practices and can be used to guide regulatory policymaking and to conduct required regulatory impact analysis of potential regulations. C1 [Viator, Catherine L.; Cates, Sheryl C.; Karns, Shawn A.; Muth, Mary K.] RTI Int, 3040 East Cornwallis Rd, Res Triangle Pk, NC 27709 USA. [Noyes, Gary] US Food Safety & Inspect Serv, USDA, 355 East St SW, Washington, DC 20024 USA. [Noyes, Gary] Consumer Financial Protect Bur, 1275 First St NE, Washington, DC 20002 USA. RP Viator, CL (reprint author), RTI Int, 3040 East Cornwallis Rd, Res Triangle Pk, NC 27709 USA. EM viator@rti.org FU U.S. Department of Agriculture (USDA), Food Safety and Inspection Service (FSIS) [AG-3A94-K-12-0115] FX This work was funded by the U.S. Department of Agriculture (USDA), Food Safety and Inspection Service (FSIS; contract AG-3A94-K-12-0115). All views expressed here are those of the authors and not necessarily of USDA-FSIS. We acknowledge Jenna Brophy, Michaela Coglaiti, and Becky Durocher (RTI International) for their assistance with survey administration and Peter Siegel, also with RTI International, for his assistance with the sample design and analysis procedures. NR 14 TC 0 Z9 0 U1 10 U2 10 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUL PY 2016 VL 79 IS 7 BP 1210 EP 1215 DI 10.4315/0362-028X.JFP-15-520 PG 6 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DU0PU UT WOS:000381907800016 PM 27357041 ER PT J AU Hudson, LK Harrison, MA Berrang, ME Jones, DR AF Hudson, Lauren K. Harrison, Mark A. Berrang, Mark E. Jones, Deana R. TI Alternative Antimicrobial Commercial Egg Washing Procedures SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Chlorine; Chlorine stabilizer; Eggs; Egg wash; Salmonella ID SHELL EGGS; SALMONELLA-ENTERITIDIS; CROSS-CONTAMINATION; WATER; EFFICACY; TEMPERATURES; INOCULATION; PENETRATION; CHEMICALS; STRENGTH AB Commercial table eggs are washed prior to packaging. Standard wash procedures use an alkaline pH and warm water. If a cool water method could be developed that would still provide a microbiologically safe egg, the industry may save energy costs associated with water heating. Four wash procedures were evaluated for Salmonella reduction: pH 11 at 48.9 degrees C (industry standard), pH 11 at ambient temperature (similar to 20 degrees C), pH 6 at 48.9 degrees C, and pH 6 at ambient temperature. Alkaline washes contained potassium hydroxide-based detergent, while pH 6 washes contained approximately 200 ppm of chlorine and a proprietary chlorine stabilizer (T-128). When eggs were inoculated by immersion in a cell suspension of Salmonella Enteritidis and Salmonella Typhimurium, all treatments resulted in a slight and similar reduction of Salmonella numbers (approximately 0.77 log CFU/ ml of shell emulsion reduction). When eggs were inoculated by droplet on the shell surface, Salmonella counts were reduced by approximately 5 log CFU when washed with chlorine plus the chlorine stabilizer at both temperatures and with the alkaline wash at the high temperature. The reductions in Salmonella by these treatments were not significantly (P > 0.05) different from each other but were significantly (P < 0.05) more than the reduction observed for the 20 degrees C alkaline treatment and 20 degrees C control water treatments. Ambient temperature acidic washes reduced Salmonella contamination to the same degree as the standard pH 11 warm water wash and may be a viable option to reduce cost, increase shelf life, and slow pathogen growth in and on shell eggs. C1 [Hudson, Lauren K.; Harrison, Mark A.] Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA. [Berrang, Mark E.] ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, USDA, Athens, GA 30605 USA. [Jones, Deana R.] ARS, Egg Safety & Qual Res Unit, USDA, 950 Coll Stn Rd, Athens, GA 30605 USA. RP Harrison, MA (reprint author), Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA. EM mahfst@uga.edu FU Georgia Agricultural Experiment Stations; SmartWash Solutions FX This material is based upon work supported by Georgia Agricultural Experiment Stations and SmartWash Solutions. Thanks to Stephen Norris, Robin Woodroof, Garrett Ward, Steven Knapp, Eric Adams, Gwen Hirsch, Suejee Park, Charlotte Steininger, Cathy Micali, and Austin Bernard for technical assistance. NR 33 TC 0 Z9 0 U1 2 U2 2 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUL PY 2016 VL 79 IS 7 BP 1216 EP 1220 DI 10.4315/0362-028X.JFP-15-423 PG 5 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DU0PU UT WOS:000381907800017 PM 27357042 ER PT J AU Cook, N Knight, A Richards, GP AF Cook, Nigel Knight, Angus Richards, Gary P. TI Persistence and Elimination of Human Norovirus in Food and on Food Contact Surfaces: A Critical Review SO JOURNAL OF FOOD PROTECTION LA English DT Review DE Elimination; Food; Norovirus; Persistence; Surfaces; Water ID VIRUS-LIKE PARTICLES; HIGH HYDROSTATIC-PRESSURE; HUMAN GII.4 NOROVIRUS; HEPATITIS-A VIRUS; MURINE NOROVIRUS; FELINE-CALICIVIRUS; NORWALK VIRUS; ENTERIC VIRUSES; STAINLESS-STEEL; BACTERIAL-CONTAMINATION AB This critical review addresses the persistence of human norovirus (NoV) in water, shellfish, and processed meats; on berries, herbs, vegetables, fruits, and salads; and on food contact surfaces. The review focuses on studies using NoV; information from studies involving only surrogates is not included. It also addresses NoV elimination or inactivation by various chemical, physical, or processing treatments. In most studies, persistence or elimination was determined by detection and quantification of the viral genome, although improved methods for determining infectivity have been proposed. NoV persisted for 60 to 728 days in water, depending on water source. It also persisted on berries, vegetables, and fruit, often showing <1-log reduction within 1 to 2 weeks. NoV was resilient on carpets, Formica, stainless steel, polyvinyl chloride, and ceramic surfaces; during shellfish depuration; and to repeated freeze-thaw cycles. Copper alloy surfaces may inactivate NoV by damaging viral capsids. Disinfection was achieved for some foods or food contact surfaces using chlorine, calcium or sodium hypochlorite, chlorine dioxide, high hydrostatic pressure, high temperatures, pH values >8.0, freeze-drying, and UV radiation. Ineffective disinfectants included hydrogen peroxide, quaternary ammonium compounds, most ethanol-based disinfectants, and antiseptics at normally used concentrations. Thorough washing of herbs and produce was effective in reducing, but not eliminating, NoV in most products. Washing hands with soap generally reduced NoV by <2 log. Recommendations for future research needs are provided. C1 [Cook, Nigel] Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England. [Knight, Angus] Leatherhead Food Res, Leatherhead KT22 7RY, Surrey, England. [Richards, Gary P.] ARS, USDA, Dover, DE 19901 USA. RP Richards, GP (reprint author), ARS, USDA, Dover, DE 19901 USA. EM gary.richards@ars.usda.gov FU United Kingdom Food Standards Agency [FS101120]; U.S. Department of Agriculture, Agricultural Research Service under CRIS project [8072-42000-065-00D] FX This work was funded by the United Kingdom Food Standards Agency, project FS101120 (A. Knight, N. Cook) and by the U.S. Department of Agriculture, Agricultural Research Service under CRIS project 8072-42000-065-00D (G. Richards). We thank Jonathan Stein for assistance with literature acquisition. NR 110 TC 1 Z9 1 U1 25 U2 25 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUL PY 2016 VL 79 IS 7 BP 1273 EP 1294 DI 10.4315/0362-028X.JFP-15-570 PG 22 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DU0PU UT WOS:000381907800026 PM 27357051 ER PT J AU Buffington, ML Copeland, RS AF Buffington, Matthew L. Copeland, Robert S. TI REDESCRIPTION OF HELORUS RUFICORNIS FORSTER (HYMENOPTERA: HELORIDAE), WITH A NEW SYNONYMY AND NEW AFROTROPICAL SPECIMEN RECORDS SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Article DE parasitoid; Helorus elgoni; Kenya; Burundi AB Research up to this point has recorded two species of Afrotropical Heloridae: Helorus ruficornis Forster and H. elgoni Risbec. After examining recently collected specimens from Kenya and Burundi, we have been able to better understand intraspecific variation within Helorus ruficornis, and as a result, we hereby synonymize H. elgoni with H. ruficornis, redescribe H. ruficornis, and illustrate the holotype of H. elgoni for the first time. We also discuss new collection records for Afrotropical Heloridae, as well as possible habitat preferences. C1 [Buffington, Matthew L.] USDA ARS, Systemat Entomol Lab, Smithsonian Inst, Natl Museum Nat Hist, 10th & Constitut Ave NW, Washington, DC 20560 USA. [Copeland, Robert S.] ICIPE, POB 30772, Nairobi, Kenya. [Copeland, Robert S.] Natl Museums Kenya, Div Invertebrate Zool, POB 40658, Nairobi 00100, Kenya. RP Buffington, ML (reprint author), USDA ARS, Systemat Entomol Lab, Smithsonian Inst, Natl Museum Nat Hist, 10th & Constitut Ave NW, Washington, DC 20560 USA. EM matt.buffington@ars.usda.gov; rcopeland@icipe.org FU government of Finland; Systematic Entomology Laboratory, ARS-USDA FX We thank Dr. Claire Villemont and Miss Agnele Tourney-Albey (Museum national d'Histoire naturelle, Paris, France (MNHN)) for assistance with the loan of the holotype of Helorus elgoni, and to Dr. Andrew Bennett (Canadian National Collection of Insects, Ottawa, Canada) for the loan of African H. ruficornis. We also thank Taina Litwak (Systematic Entomology Laboratory, ARS-USDA) for the gorgeous digital painting of Helorus ruficornis in Figure 1. We wish to thank the government of Finland for partial funding of our research through a grant to ICIPE (the CHIESA Project; Climate Change Impacts on Ecosystem Services and Food Security in Eastern Africa). Many thanks to the Kenya Wildlife Service (KWS), and in particular to Dr Richard Bagine, the KWS Head of Research, for permission to sample in Kenyan national parks and reserves. Thanks also to the director, Kenya Forest Service for permission to sample in the forests of Taita Hills. RSC wishes to thank the director of l'Institut National pour l'Environnement et la Conservation de la Nature (INECN) for permission to sample insects in Burundi. And special thanks to my Burundi colleagues and friends, Benoit Nzigidahera of INECN and Evariste Nkubaye of the Institut des Sciences Agronomiques du Burundi for their great help with the Malaise trap project. Juliet Muruiki provided excellent technical support in the field and in the laboratory. Jackson Kimani of ICIPE's Earth Observation Unit made the excellent map (Figure 19). MLB was supported by the Systematic Entomology Laboratory, ARS-USDA. Dr. Lubomir Masner (CNCI) offered helpful comments in the early portion of this project. Istvan Miko (Pennsylvania State University, USA) and Simon van Noort (Iziko South African Museum, Cape Town, RSA) provided reviews that greatly improved this manuscript. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer. NR 11 TC 0 Z9 0 U1 2 U2 2 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD JUL PY 2016 VL 118 IS 3 BP 330 EP 344 DI 10.4289/0013-8797.118.3.330 PG 15 WC Entomology SC Entomology GA DS5PP UT WOS:000380834800002 ER PT J AU Gagne, RJ AF Gagne, Raymond J. TI A NEW GENUS AND SPECIES OF CECIDOMYIIDAE (DIPTERA) FROM LEAF BLISTER GALLS ON RIBES (GROSSULARIACEAE) IN NORTH AMERICA SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Article DE gall midges; Cecidomyiinae; Lasiopteridi; Holarctic; gooseberry; currant ID MIDGES AB Ribesia sarae Gagne, new genus, new species (Diptera: Cecidomyiidae), is described from simple leaf blister galls on Ribes aureum (Grossulariaceae) from Montana, USA. The female abdomen of Ribesia is superficially similar to that of Cystiphora Kieffer and Sackenomyia Felt. The three genera are compared. Because of strong differences found in their male terminalia it appears that any similarities in the female abdomens are the result of convergence. Sackenomyia ribesifolia Fedotova 1987 from Ribes in Kazakhstan is a new combination in Ribesia. C1 [Gagne, Raymond J.] ARS, Systemat Entomol Lab, USDA, Smithsonian Inst MRC 168, POB 37012, Washington, DC 20013 USA. RP Gagne, RJ (reprint author), ARS, Systemat Entomol Lab, USDA, Smithsonian Inst MRC 168, POB 37012, Washington, DC 20013 USA. EM raymond.gagne@ars.usda.gov NR 10 TC 0 Z9 0 U1 0 U2 0 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD JUL PY 2016 VL 118 IS 3 BP 354 EP 362 DI 10.4289/0013-8797.118.3.354 PG 9 WC Entomology SC Entomology GA DS5PP UT WOS:000380834800004 ER PT J AU Henry, TJ AF Henry, Thomas J. TI A NEW TILLANDSIA-FEEDING SPECIES OF THE ECCRITOTARSINE PLANT BUG GENUS TENTHECORIS (HEMIPTERA: HETEROPTERA: MIRIDAE: BRYOCORINAE) FROM THE SOUTHEASTERN UNITED STATES SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Article DE Insect; Hemiptera; new species; adventive; host; South Carolina; United States ID SPANISH MOSS; USNEOIDES; SPIDERS; INSECTS; GENERA AB The new eccritotarsine plant bug, Tenthecoris tillandsiae (Heteroptera: Miridae: Bryocorinae), is described from specimens taken on Spanish moss, Til-landsia usneoides, growing on various deciduous and coniferous trees, in southeastern South Carolina, USA. This species represents the first record of the genus Tenthecoris Scott from the United States and the first host record from a bromeliad. All other species of Tenthecoris (commonly called orchid bugs) having known hosts are associated with orchids (Orchidaceae). The new species is diagnosed, described, and illustrated, including male genitalia, to help distinguish it from other members of the genus. C1 [Henry, Thomas J.] ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, Washington, DC 20013 USA. RP Henry, TJ (reprint author), ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, Washington, DC 20013 USA. EM thomas.henry@ars.usda.gov NR 30 TC 0 Z9 0 U1 3 U2 3 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD JUL PY 2016 VL 118 IS 3 BP 363 EP 372 DI 10.4289/0013-8797.118.3.363 PG 10 WC Entomology SC Entomology GA DS5PP UT WOS:000380834800005 ER PT J AU Smith, DR AF Smith, David R. TI NEW SPECIES AND RECORDS OF SAWFLIES (HYMENOPTERA: ARGIDAE, PERGIDAE, TENTHREDINIDAE) FROM ARIZONA SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Article DE new species; new records; Ametastegia; Eustromboceros; Periclista; Stromboceridea AB Zynzus truncatus Smith, n. sp. (Argidae), Acordulecera irwini Smith, n. sp. (Pergidae), and Nematus grahami Smith, n. sp. (Tenthredinidae), are described from southern Arizona. Arizona records for ten other sawfly species of Argidae, Pergidae, and Tenthredinidae from recent collections are given. C1 [Smith, David R.] ARS, Natl Museum Nat Hist, USDA, Systemat Entomol Lab,Smithsonian Inst, MRC 168,POB 37012, Washington, DC 20013 USA. RP Smith, DR (reprint author), ARS, Natl Museum Nat Hist, USDA, Systemat Entomol Lab,Smithsonian Inst, MRC 168,POB 37012, Washington, DC 20013 USA. EM sawfly2@aol.com NR 8 TC 0 Z9 0 U1 1 U2 1 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD JUL PY 2016 VL 118 IS 3 BP 393 EP 403 DI 10.4289/0013-8797.118.3.393 PG 11 WC Entomology SC Entomology GA DS5PP UT WOS:000380834800009 ER PT J AU Talamas, EJ Johnston-Jordan, D Buffington, ML AF Talamas, Elijah J. Johnston-Jordan, Dylan Buffington, Matthew L. TI CALLISCELIO ASHMEAD EXPANDS (HYMENOPTERA: SCELIONIDAE) SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Article DE Platygastroidea; parasitoid; synonymy; morphology ID PLATYGASTROIDEA; PROCTOTRUPOIDEA AB The generic concept of Calliscelio Ashmead is expanded to include variation in the shape of the metascutellum, the development of propodeal carinae, the presence of an epomial carina, the presence of malar striae, and the position of the toruli on the frons. This expansion follows circumscription of the genus based on the prioniform sensillum on the mandible, a character that is unique within Platygastroidea. We consider the prioniform state of the sensillum to be a synapo-morphy for the genus, and treat Crama Galloway, Lispoteleia Galloway, Xentor Masner and Johnson, and Yunkara Galloway as junior synonyms of Calliscelio; the species of these genera are transferred herein. Calliscelio yunkara Talamas is provided as a replacement name for Yunkara inornata Galloway. A lectotype is designated for Calliscelio albicoxa (Dodd). C1 [Talamas, Elijah J.; Buffington, Matthew L.] ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, POB 37012,MRC 168, Washington, DC 20013 USA. [Johnston-Jordan, Dylan] Smithsonian Inst, Natl Museum Nat Hist, POB 37012,MRC 168, Washington, DC 20013 USA. RP Talamas, EJ (reprint author), ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, POB 37012,MRC 168, Washington, DC 20013 USA. EM elijah.talamas@ars.usda.gov FU Systematic Entomology Lab, USDA-ARS FX We are grateful to: Andy Bennett (CNC), Nicole Fisher (ANIC), and Chris Burwell (QM) for the specimens that made this study posible; Joe Cora (OSUC) for database support; Huayan Chen (OSUC) and Rajmohana Keloth (Zoological Survey of India) for examination of specimens; Lubomir Masner (CNC) for taxonomic input and specimens; Norman Johnson (OSUC) for taxonomic input and photographs of Calliscelio albicoxa; and to Miguel Alonso-Zarazaga (Museo Nacional de Ciencias Naturales, Madrid, Spain) for nomenclatural advice. This work was made possible by funding from the Systematic Entomology Lab, USDA-ARS. The USDA does not endorse any commercial product mentioned in this research. USDA is an equal opportunity provider and employer. NR 47 TC 1 Z9 1 U1 0 U2 0 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD JUL PY 2016 VL 118 IS 3 BP 404 EP 423 DI 10.4289/0013-8797.118.3.404 PG 20 WC Entomology SC Entomology GA DS5PP UT WOS:000380834800010 ER PT J AU Milnes, JM Wiman, NG Talamas, EJ Brunner, JF Hoelmer, KA Buffington, ML Beers, EH AF Milnes, Joshua M. Wiman, Nik G. Talamas, Elijah J. Brunner, Jay F. Hoelmer, Kim A. Buffington, Matthew L. Beers, Elizabeth H. TI Discovery of an Exotic Egg Parasitoid of the Brown Marmorated Stink Bug, Halyomorpha halys (Stal) in the Pacific Northwest SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Editorial Material ID HETEROPTERA-PENTATOMIDAE; ASHMEAD HYMENOPTERA; SCELIONIDAE; HEMIPTERA; AMERICA; BIOLOGY; CHINA C1 [Milnes, Joshua M.; Brunner, Jay F.; Beers, Elizabeth H.] Washington State Univ, Ctr Tree Fruit Res & Extens, Wenatchee, WA 98801 USA. [Wiman, Nik G.] Oregon State Univ, Dept Hort, North Willamette Res & Extens Ctr, Aurora, OR USA. [Talamas, Elijah J.; Buffington, Matthew L.] USDA ARS, Systemat Entomol Lab, Natl Museum Nat Hist, Washington, DC USA. [Hoelmer, Kim A.] USDA ARS, Beneficial Insects Intro Res Lab, Newark, DE USA. RP Milnes, JM (reprint author), Washington State Univ, Ctr Tree Fruit Res & Extens, Wenatchee, WA 98801 USA. EM joshua.milnes@wsu.edu; Nik.Wiman@oregonstate.edu; elijah.talamas@ARS.USDA.GOV; jfb@wsu.edu; Kim.Hoelmer@ARS.USDA.GOV; Matt.Buffington@ARS.USDA.GOV; ebeers@wsu.edu NR 15 TC 1 Z9 1 U1 2 U2 2 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD JUL PY 2016 VL 118 IS 3 BP 466 EP 470 DI 10.4289/0013-8797.118.3.466 PG 5 WC Entomology SC Entomology GA DS5PP UT WOS:000380834800015 ER PT J AU Green, BT Lee, ST Welch, KD Cook, D Kem, WR AF Green, Benedict T. Lee, Stephen T. Welch, Kevin D. Cook, Daniel Kem, William R. TI Activation and Desensitization of Peripheral Muscle and Neuronal Nicotinic Acetylcholine Receptors by Selected, Naturally-Occurring Pyridine Alkaloids SO TOXINS LA English DT Article DE anabaseine; anabasine; epibatidine; nicotinic acetylcholine receptor; nicotine; pyridine alkaloid ID PREGNANT GOAT MODEL; FETAL MOVEMENT; CELLS; INHIBITION; PIPERIDINE; NEUROBIOLOGY; FLUORESCENCE; ANABASEINE; AGONIST; POTENT AB Teratogenic alkaloids can cause developmental defects due to the inhibition of fetal movement that results from desensitization of fetal muscle-type nicotinic acetylcholine receptors (nAChRs). We investigated the ability of two known teratogens, the piperidinyl-pyridine anabasine and its 1,2-dehydropiperidinyl analog anabaseine, to activate and desensitize peripheral nAChRs expressed in TE-671 and SH-SY5Y cells. Activation-concentration response curves for each alkaloid were obtained in the same multi-well plate. To measure rapid desensitization, cells were first exposed to five potentially-desensitizing concentrations of each alkaloid in log(10) molar increments from 10 nM to 100 mu M and then to a fixed concentration of acetylcholine (ACh), which alone produces near-maximal activation. The fifty percent desensitization concentration (DC50) was calculated from the alkaloid concentration-ACh response curve. Agonist fast desensitization potency was predicted by the agonist potency measured in the initial response. Anabaseine was a more potent desensitizer than anabasine. Relative to anabaseine, nicotine was more potent to autonomic nAChRs, but less potent to the fetal neuromuscular nAChRs. Our experiments have demonstrated that anabaseine is more effective at desensitizing fetal muscle-type nAChRs than anabasine or nicotine and, thus, it is predicted to be more teratogenic. C1 [Green, Benedict T.; Lee, Stephen T.; Welch, Kevin D.; Cook, Daniel] ARS, Poisonous Plant Res Lab, USDA, 1150 E 1400 N, Logan, UT 84341 USA. [Kem, William R.] Univ Florida, Dept Pharmacol & Therapeut, Coll Med, 1200 Newell Rd, Gainesville, FL 32610 USA. RP Green, BT (reprint author), ARS, Poisonous Plant Res Lab, USDA, 1150 E 1400 N, Logan, UT 84341 USA. EM ben.green@ars.usda.gov; stephen.lee@ars.usda.gov; kevin.welch@ars.usda.gov; daniel.cook@ars.usda.gov; wrkem@ufl.edu FU United States Department of Agriculture, Agricultural Research Service (USDA/ARS) FX The authors thank Jim Pfister for his statistical advice, Isabelle McCollum, for her helpful comments and technical expertise, and David R. Brown for his advice in preparing the manuscript. This research was supported by United States Department of Agriculture, Agricultural Research Service (USDA/ARS). NR 26 TC 0 Z9 0 U1 3 U2 3 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2072-6651 J9 TOXINS JI Toxins PD JUL PY 2016 VL 8 IS 7 AR 204 DI 10.3390/toxins8070204 PG 12 WC Toxicology SC Toxicology GA DS4PU UT WOS:000380763800014 ER PT J AU Luppold, WG Bumgardner, MS AF Luppold, William G. Bumgardner, Matthew S. TI US HARDWOOD LUMBER CONSUMPTION AND INTERNATIONAL TRADE FROM 1991 TO 2014 SO WOOD AND FIBER SCIENCE LA English DT Article DE Hardwood lumber use; hardwood consumption trends; hardwood industry groupings; appearance-based uses; industrial uses; US hardwood trade ID WOOD MATERIAL USE; UNITED-STATES; FURNITURE INDUSTRY; PRODUCTS; LOG AB Apparent US hardwood lumber consumption (developed from production, import, and export data) was contrasted with estimated consumption based on employment data and lumber utilization coefficients. The two methods of measuring domestic consumption provided similar results, but the use of employment data allowed for a comparison of appearance lumber vs industrial lumber use. Consumption of both appearance and industrial lumber increased between 1991 and 2000 as imported lumber augmented domestic lumber production. Exports increased during the 1990s but at a lower rate than domestic consumption. Beginning in 2000, consumption of appearance lumber started to decrease because of globalization of the furniture industry followed by a decline in US home construction. The 2008-2009 recession was associated with declines for all segments of domestic consumption and a decline in exports. Domestic consumption of appearance lumber continued at depressed levels until 2012. Exports and industrial consumption increased after 2009. In 2014, industrial users accounted for 51% of domestic lumber consumption, and exports represented 37% of consumption of appearance lumber. C1 [Luppold, William G.] US Forest Serv, Northern Res Stn, Princeton, WV 24740 USA. [Bumgardner, Matthew S.] US Forest Serv, Northern Res Stn, Delaware, OH USA. RP Luppold, WG (reprint author), US Forest Serv, Northern Res Stn, Princeton, WV 24740 USA. EM wluppold@fs.fed.us; mbumgardner@fs.fed.us NR 23 TC 0 Z9 0 U1 3 U2 3 PU SOC WOOD SCI TECHNOL PI MADISON PA ONE GIFFORD PINCHOT DR, MADISON, WI 53705 USA SN 0735-6161 J9 WOOD FIBER SCI JI Wood Fiber Sci. PD JUL PY 2016 VL 48 IS 3 BP 162 EP 170 PG 9 WC Forestry; Materials Science, Paper & Wood; Materials Science, Textiles SC Forestry; Materials Science GA DT9UB UT WOS:000381846700003 ER PT J AU Milne, E Aynekulu, E Bationo, A Batjes, NH Boone, R Conant, R Davies, J Hanan, N Hoag, D Herrick, JE Knausenberger, W Neely, C Njoka, J Ngugi, M Parton, B Paustian, K Reid, R Said, M Shepherd, K Swift, D Thornton, P Williams, S Miller, S Nkonya, E AF Milne, Eleanor Aynekulu, Ermias Bationo, Andre Batjes, Niels H. Boone, Randall Conant, Rich Davies, Jonathan Hanan, Niall Hoag, Dana Herrick, Jeffrey E. Knausenberger, Walter Neely, Constance Njoka, Jesse Ngugi, Moffatt Parton, Bill Paustian, Keith Reid, Robin Said, Mohamed Shepherd, Keith Swift, David Thornton, Philip Williams, Stephen Miller, Sue Nkonya, Ephraim TI Grazing lands in Sub-Saharan Africa and their potential role in climate change mitigation: What we do and don't know SO ENVIRONMENTAL DEVELOPMENT LA English DT Article ID SOIL CARBON SEQUESTRATION; BENEFITS C1 [Milne, Eleanor; Boone, Randall; Conant, Rich; Hoag, Dana; Parton, Bill; Paustian, Keith; Reid, Robin; Swift, David; Williams, Stephen; Miller, Sue] Colorado State Univ, Ft Collins, CO 80523 USA. [Knausenberger, Walter; Ngugi, Moffatt] USAID, Washington, DC USA. [Aynekulu, Ermias; Neely, Constance; Shepherd, Keith] ICRAF World Agroforestry Ctr, Nairobi, Kenya. [Bationo, Andre] Alliance Green Revolut Africa, Accra, Ghana. [Batjes, Niels H.] ISRIC World Soil Informat, Wageningen, Netherlands. [Davies, Jonathan] IUCN, Gland, Switzerland. [Hanan, Niall] South Dakota State Univ, Brookings, SD USA. [Herrick, Jeffrey E.] USDA ARS, Washington, DC 20250 USA. [Neely, Constance] FAO, Rome, Italy. [Njoka, Jesse] Univ Nairobi, Nairobi, Kenya. [Said, Mohamed; Thornton, Philip] Univ Nairobi, ILRI, CSDES, Nairobi, Kenya. [Nkonya, Ephraim] IFPRI, Washington, DC 20006 USA. RP Milne, E (reprint author), Colorado State Univ, Ft Collins, CO 80523 USA. RI Batjes, Niels/F-7195-2010; OI Batjes, Niels/0000-0003-2367-3067; Hanan, Niall/0000-0002-9130-5306 NR 17 TC 0 Z9 0 U1 4 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2211-4645 EI 2211-4653 J9 ENVIRON DEV JI Environ. Dev. PD JUL PY 2016 VL 19 BP 70 EP 74 DI 10.1016/j.envdev.2016.06.001 PG 5 WC Environmental Sciences SC Environmental Sciences & Ecology GA DT6WR UT WOS:000381625800007 ER PT J AU Taylor, M Klaiber, HA Kuchler, F AF Taylor, Mykel Klaiber, H. Allen Kuchler, Fred TI Changes in US consumer response to food safety recalls in the shadow of a BSE scare SO FOOD POLICY LA English DT Article DE Consumer demand; Regulatory power; Food safety; Ground beef; Recall ID HOUSEHOLD SCANNER DATA; RISK PERCEPTIONS; DEMAND ANALYSIS; UNITED-STATES; MEAT DEMAND; BEEF; INFORMATION; HOMESCAN; DISEASE; MODELS AB In December 2003, Bovine Spongiform Encephalopathy (BSE) was discovered in the United States. This food safety event received extensive media coverage and prompted changes in regulatory controls. Using a panel selection model, we show that prior to December 2003, ground beef recalls had no impact on household purchases of ground beef, even for households that were located in the recall-defined geographic areas. However, we find robust evidence that the 2003 BSE event caused a change in the way people view and respond to recalls of ground beef, a change that persisted for at, least two years following the BSE event. The average impact of a ground beef recall in the post-BSE period is a 0.26 1b per person reduction in retail purchases of ground beef. A decline in purchases of this magnitude would result in over $97 million in losses to the beef industry in a two week period following a nationwide recall. This dwarfs the economic impacts of directly removing recalled beef from supply chains and provides FSIS increased regulatory power due to higher overall industry costs associated with food safety violations. Published by Elsevier Ltd. C1 [Taylor, Mykel] Kansas State Univ, Dept Agr Econ, 342 Waters Hall, Manhattan, KS 66506 USA. [Klaiber, H. Allen] Ohio State Univ, Dept Agr Environm & Dev Econ, 333 Agr Adm Bldg, Columbus, OH 43210 USA. [Kuchler, Fred] USDA, Econ Res Serv, 1400 Independence Ave,SW Mail Stop 1800, Washington, DC 20250 USA. RP Taylor, M (reprint author), Kansas State Univ, Dept Agr Econ, 342 Waters Hall, Manhattan, KS 66506 USA. EM mtaylor@ksu.edu NR 32 TC 1 Z9 1 U1 12 U2 13 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0306-9192 EI 1873-5657 J9 FOOD POLICY JI Food Policy PD JUL PY 2016 VL 62 BP 56 EP 64 DI 10.1016/j.foodpol.2016.04.005 PG 9 WC Agricultural Economics & Policy; Economics; Food Science & Technology; Nutrition & Dietetics SC Agriculture; Business & Economics; Food Science & Technology; Nutrition & Dietetics GA DS1XA UT WOS:000380417200005 ER PT J AU Liefert, WM Westcott, PC AF Liefert, William M. Westcott, Paul C. TI Modifying agricultural export taxes to make them less market-distorting SO FOOD POLICY LA English DT Article DE Export taxes; Export restrictions; Agricultural trade; Trade policy; Food security AB From 2006 to 2014, close to 40 countries levied an export tax on at least one agricultural product. Export taxes impose costs on the global economy by reducing total world welfare compared to free trade, and in particular hurt the tax-imposing country's own domestic producers of the taxed good. Yet countries with export taxes have objectives that might make it unlikely for them to eliminate, or even reduce, the taxes. This article examines how a conventional export tax could be modified to make it less market-distorting, and thereby less welfare-diminishing, for both the tax-imposing country and the rest of the world. From a total world welfare perspective, the modified policy analyzed here is a "second best" alternative to the first best policy of abolishing the export tax and allowing free trade, but nonetheless it improves global economic welfare compared to the standard export tax. The modified policy discussed here achieves the same economic objectives as the tax, such as reducing the domestic price of the exported good, increasing domestic purchases, and raising revenue, but also generates additional exports beyond the volume that occurs under the tax alone. Also, the tax does not involve any government subsidies to producers or consumers. The main original feature of the modified export tax policy therefore is that it not only increases net economic welfare (compared to the unmodified tax scenario), but does so in a way that does not reduce the welfare of any economic group. Published by Elsevier Ltd. C1 [Liefert, William M.; Westcott, Paul C.] ERS, Market & Trade Econ Div, USDA, 355 E St SW, Washington, DC 20024 USA. RP Liefert, WM (reprint author), ERS, Market & Trade Econ Div, USDA, 355 E St SW, Washington, DC 20024 USA. EM wliefert@ers.usda.gov NR 22 TC 0 Z9 0 U1 4 U2 4 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0306-9192 EI 1873-5657 J9 FOOD POLICY JI Food Policy PD JUL PY 2016 VL 62 BP 65 EP 77 DI 10.1016/j.foodpol.2016.04.001 PG 13 WC Agricultural Economics & Policy; Economics; Food Science & Technology; Nutrition & Dietetics SC Agriculture; Business & Economics; Food Science & Technology; Nutrition & Dietetics GA DS1XA UT WOS:000380417200006 ER PT J AU do Evangelho, JA Berrios, JD Pinto, VZ Antunes, MD Vanier, NL Zavareze, ED AF do Evangelho, Jarine Amaral Berrios, Jose de J. Pinto, Vania Zanella Antunes, Mariana Dias Vanier, Nathan Levien Zavareze, Elessandra da Rosa TI Antioxidant activity of black bean (Phaseolus vulgaris L.) protein hydrolysates SO FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE alcalase; antioxidant; molecular weight; pepsin; protein ID FUNCTIONAL-PROPERTIES; ENZYMATIC-HYDROLYSIS; PEPTIDES; CHICKPEA AB The objective of this work was to study the effect of enzymatic hydrolysis of black bean protein concentrate using different enzymes. Bean proteins were extracted and hydrolyzed over a period of 120 min using the enzymes pepsin or alcalase. The protein hydrolysates' molecular weight was assayed by electrophoresis and the antioxidant activity was evaluated by the capturing methods of free radicals ABTS(center dot)+ and DPPH. Electrophoretic results showed that the bands above 50 kDa disappeared, when the beans protein was subjected to hydrolysis with pepsin. The bean protein hydrolysate obtained by hydrolysis with alcalase enzyme, showed higher antioxidant activity for inhibition of the radical ABTS(center dot)+. However, the hydrolysates obtained by hydrolysis with pepsin had higher antioxidant activity for inhibition of the radical DPPH. The use of pepsin and alcalase enzymes, under the same reaction time, produced black bean protein hydrolysates with different molecular weight profiles and superior antioxidant activity than the native bean protein. C1 [do Evangelho, Jarine Amaral; Pinto, Vania Zanella; Antunes, Mariana Dias; Vanier, Nathan Levien; Zavareze, Elessandra da Rosa] Univ Fed Pelotas UFPel, Pelotas, RS, Brazil. [Berrios, Jose de J.] USDA, Albany, CA USA. RP do Evangelho, JA (reprint author), Univ Fed Pelotas UFPel, Pelotas, RS, Brazil. EM jarineamaral@hotmail.com FU CNPq; CAPES; FAPERGS; SCT-RS; Polos Tecnologicos FX We would like to thank to CNPq by project financing, CAPES, FAPERGS, SCT-RS and Polos Tecnologicos for the scholarship and financial. NR 25 TC 0 Z9 0 U1 18 U2 19 PU SOC BRASILEIRA CIENCIA TECNOLOGIA ALIMENTOS PI CAMPINAS PA AV BRASIL 2880, CAXIA POSTAL 271 CEP 13001-970, CAMPINAS, SAO PAULO 00000, BRAZIL SN 0101-2061 EI 1678-457X J9 FOOD SCI TECH-BRAZIL JI Food Sci. Technol. PD JUL PY 2016 VL 36 SU 1 BP 23 EP 27 DI 10.1590/1678-457X.0047 PG 5 WC Food Science & Technology SC Food Science & Technology GA DS5NB UT WOS:000380827900006 ER PT J AU Keane, RE AF Keane, Robert E. TI Spatiotemporal Variability of Wildland Fuels in US Northern Rocky Mountain Forests SO FORESTS LA English DT Review DE range; semi-variogram; fuel deposition; decomposition; fuel component; vegetation development ID COARSE WOODY DEBRIS; SURFACE FUEL; FIRE MANAGEMENT; UNITED-STATES; SPATIAL HETEROGENEITY; REDUCTION TREATMENTS; SAMPLING TECHNIQUES; CONIFER FOREST; CLIMATE-CHANGE; ECOSYSTEMS AB Fire regimes are ultimately controlled by wildland fuel dynamics over space and time; spatial distributions of fuel influence the size, spread, and intensity of individual fires, while the temporal distribution of fuel deposition influences fire's frequency and controls fire size. These "shifting fuel mosaics" are both a cause and a consequence of fire regimes. This paper synthesizes results from two major fuel dynamics studies that described the spatial and temporal variability of canopy and surface wildland fuel characteristics found in US northern Rocky Mountain forests. Eight major surface fuel components-four downed dead woody fuel size classes (1, 10, 100, 1000 h), duff, litter, shrub, and herb-and three canopy fuel characteristics-loading, bulk density and cover-were studied. Properties of these fuel types were sampled on nested plots located within sampling grids to describe their variability across spatiotemporal scales. Important findings were that fuel component loadings were highly variable (two to three times the mean), and this variability increased with the size of fuel particles. The spatial variability of loadings also varied by spatial scale with fine fuels (duff, litter, 1 h, 10 h) varying at scales of 1 to 5 m; coarse fuels at 10 to 150 m, and canopy fuels at 100 to 600 m. Fine fuels are more uniformly distributed over both time and space and decayed quickly, while large fuels are rare on the landscape but have a high residence time. C1 [Keane, Robert E.] US Forest Serv, Missoula Fire Sci Lab, Rocky Mt Res Stn, 5775 Highway 10 West, Missoula, MT 59808 USA. RP Keane, RE (reprint author), US Forest Serv, Missoula Fire Sci Lab, Rocky Mt Res Stn, 5775 Highway 10 West, Missoula, MT 59808 USA. EM rkeane@fs.fed.us FU USGS National Biological Service and Glacier National Park's Global Change Research Program [1430-1-9007, 1430-3-9005]; USGS CLIMET project; National Fire Plan Research monies FX This work was partially funded by the USGS National Biological Service and Glacier National Park's Global Change Research Program under Interagency Agreements 1430-1-9007 and 1430-3-9005 and the USGS CLIMET project. It was also funded using National Fire Plan Research monies. NR 55 TC 0 Z9 0 U1 5 U2 5 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD JUL PY 2016 VL 7 IS 7 AR 129 DI 10.3390/f7070129 PG 16 WC Forestry SC Forestry GA DS4SM UT WOS:000380771500002 ER PT J AU Tinkham, WT Hoffman, CM Ex, SA Battaglia, MA Saralecos, JD AF Tinkham, Wade T. Hoffman, Chad M. Ex, Seth A. Battaglia, Michael A. Saralecos, Jarred D. TI Ponderosa Pine Forest Restoration Treatment Longevity: Implications of Regeneration on Fire Hazard SO FORESTS LA English DT Article DE Torching; Crowning; fire modeling; fire behavior; seedlings; crown fuels ID WESTERN UNITED-STATES; MIXED-CONIFER FOREST; COLORADO FRONT RANGE; CROWN FIRE; ECOLOGICAL RESTORATION; PRESCRIBED FIRE; NORTHERN ARIZONA; FUEL REDUCTION; CLIMATE-CHANGE; BLACK-HILLS AB Restoration of pine forests has become a priority for managers who are beginning to embrace ideas of highly heterogeneous forest structures that potentially encourages high levels of regeneration. This study utilizes stem-mapped stands to assess how simulated regeneration timing and magnitude influence longevity of reduced fire behavior by linking growth and yield model outputs to a crown fire prediction model. Treatment longevity was assessed as return time to within 10% of pre-treatment predicted wind speeds for the onset of passive (Torching) and active (Crowning) crown fire behavior. Treatment longevity in terms of Torching and Crowning was reduced 5 years for every 550 and 150 seedlings ha(-1), respectively. Introducing regeneration as a single pulse further reduced Torching treatment longevity 10 years compared to other regeneration distributions. Crowning treatment longevity increased at higher site indices, where a 6 m increase in site index increased longevity 4.5 year. This result was contrary to expectations that canopy openings after treatments would close faster on higher productivity sites. Additionally, Torching longevity was influenced by the rate of crown recession, were reducing the recession rate decreased longevity in areas with higher site indices. These dependencies highlight a need for research exploring stand development in heterogeneous sites. C1 [Tinkham, Wade T.; Hoffman, Chad M.; Ex, Seth A.] Colorado State Univ, Dept Forest & Rangeland Stewardship, Ft Collins, CO 80524 USA. [Battaglia, Michael A.] US Forest Serv, Rocky Mt Res Stn, USDA, Ft Collins, CO 80526 USA. [Saralecos, Jarred D.] Univ Montana, Dept Forest Management, Missoula, MT 59812 USA. RP Tinkham, WT (reprint author), Colorado State Univ, Dept Forest & Rangeland Stewardship, Ft Collins, CO 80524 USA. EM Wade.Tinkham@colostate.edu; C.Hoffman@colostate.edu; seth.ex@colostate.edu; mbattaglia@fs.fed.us; jarred.saralecos@umontana.edu OI Tinkham, Wade/0000-0002-4668-7624 FU Joint Fire Science Program [13-1-04-53, 14-1-01-18]; Rocky Mountain Research Station [13-JV-11221633-058]; Colorado Forest Restoration Institute FX This work would not have been possible without the funding support provided by the Joint Fire Science Program projects 13-1-04-53, 14-1-01-18, funds provided by the Rocky Mountain Research Station agreement number 13-JV-11221633-058, and the support of the Colorado Forest Restoration Institute. NR 71 TC 0 Z9 0 U1 14 U2 14 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD JUL PY 2016 VL 7 IS 7 AR 137 DI 10.3390/f7070137 PG 19 WC Forestry SC Forestry GA DS4SM UT WOS:000380771500010 ER PT J AU Fu, T Ko, J Wall, GW Pinter, PJ Kimball, BA Ottman, MJ Kim, HY AF Fu, Tongcheng Ko, Jonghan Wall, Gerard W. Pinter, Paul J., Jr. Kimball, Bruce A. Ottman, Michael J. Kim, Han-Yong TI Simulation of climate change impacts on grain sorghum production grown under free air CO2 enrichment SO INTERNATIONAL AGROPHYSICS LA English DT Article DE climate change; crop simulation; FACE; grain yield; sorghum ID ELEVATED CO2; WATER-USE; DROUGHT; MODEL; CROP; RESPONSES; PLANTS; MAIZE; YIELD; FACE AB Potential impacts of climate change on grain sorghum (Sorghum bicolor) productivity were investigated using the CERES-sorghum model in the Decision Support System for Agrotechnology Transfer v4.5. The model was first calibrated for a sorghum cultivar grown in a free air CO2 enrichment experiment at the University of Arizona, Maricopa, Arizona, USA in 1998. The model was then validated with an independent dataset collected in 1999. The simulated grain yield, growth, and soil water of sorghum for the both years were in statistical agreement with the corresponding measurements, respectively. Neither simulated nor measured yields responded to elevated CO2, but both were sensitive to water supply. The validated model was then applied to simulate possible effects of climate change on sorghum grain yield and water use efficiency in western North America for the years 2080-2100. The projected CO2 fertilizer effect on grain yield was dominated by the adverse effect of projected temperature increases. Therefore, temperature appears to be a dominant driver of the global climate change influencing future sorghum productivity. These results suggest that an increase in water demand for sorghum production should be anticipated in a future high-CO2 world. C1 [Fu, Tongcheng; Ko, Jonghan; Kim, Han-Yong] Chonnam Natl Univ, Dept Appl Plant Sci, 77 Youngbong Ro, Kwangju 500757, South Korea. [Wall, Gerard W.; Pinter, Paul J., Jr.; Kimball, Bruce A.] ARS, US Arid Land Agr Res Ctr, USDA, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. [Ottman, Michael J.] Univ Arizona, Dept Plant Sci, Tucson, AZ 85721 USA. RP Ko, J (reprint author), Chonnam Natl Univ, Dept Appl Plant Sci, 77 Youngbong Ro, Kwangju 500757, South Korea. EM Jonghan.ko@chonnam.ac.kr FU Cooperative Research Program for Agricultural Science and Technology Development, Rural Development Administration, Republic of Korea [PJ010107] FX This work was supported by Cooperative Research Program for Agricultural Science and Technology Development (Project No. PJ010107), Rural Development Administration, Republic of Korea, 2014-2016. NR 38 TC 0 Z9 0 U1 6 U2 7 PU DE GRUYTER OPEN LTD PI WARSAW PA BOGUMILA ZUGA 32A ST, 01-811 WARSAW, POLAND SN 0236-8722 EI 2300-8725 J9 INT AGROPHYS JI Int. Agrophys. PD JUL PY 2016 VL 30 IS 3 BP 311 EP 322 DI 10.1515/intag-2016-0007 PG 12 WC Agronomy SC Agriculture GA DS4OK UT WOS:000380760200006 ER PT J AU Maragos, CM AF Maragos, Chris M. TI Multiplexed Biosensors for Mycotoxins SO JOURNAL OF AOAC INTERNATIONAL LA English DT Article ID SURFACE-PLASMON RESONANCE; LATERAL-FLOW IMMUNOASSAY; MICROSPHERE SUSPENSION ARRAY; AFLATOXIN B-1; RAPID DETECTION; FUMONISIN B-1; IMMUNOCHROMATOGRAPHIC ASSAY; DIPSTICK IMMUNOASSAY; FUSARIUM MYCOTOXINS; THROUGH IMMUNOASSAY AB Significant progress has been made in the development of biosensors that can be used to detect low-MW toxins produced by fungi (mycotoxins). The number of formats that have been investigated is impressive and is an indication of the importance attached to finding easy-to-use, accurate, and rapid methods for detecting these toxins in commodities and foods. This review explores the details of multiplexed biosensors based on many formats, including multiplexed immunoassays, suspension arrays, membrane-based devices (flow-through and immunochromatographic), and planar microarrays. Each assay format has its own strengths and areas that need improvement. Certain formats, such as multiplexed immunochromatographic devices, are well developed and relatively easy to use, and in some cases, commercial products are being sold. Others, such as the suspension arrays and microarrays, are laboratory-based assays that, although more complicated, are also more amenable to a larger scale of multiplexing. The diversity of such efforts and the multitude of formats under investigation suggest that multiple solutions will be found to satisfy the need for multiplexed toxin detection. C1 [Maragos, Chris M.] ARS, USDA, Mycotoxin Prevent & Appl Microbiol Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. RP Maragos, CM (reprint author), ARS, USDA, Mycotoxin Prevent & Appl Microbiol Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM chris.maragos@ars.usda.gov FU U.S. Department of Agriculture (USDA) [5010-42000-045-00D] FX The author received support from U.S. Department of Agriculture (USDA) project 5010-42000-045-00D. NR 65 TC 1 Z9 1 U1 12 U2 15 PU AOAC INT PI GAITHERSBURG PA 481 N FREDRICK AVE, STE 500, GAITHERSBURG, MD 20877-2504 USA SN 1060-3271 EI 1944-7922 J9 J AOAC INT JI J. AOAC Int. PD JUL-AUG PY 2016 VL 99 IS 4 BP 849 EP 860 DI 10.5740/jaoacint.16-0112 PG 12 WC Chemistry, Analytical; Food Science & Technology SC Chemistry; Food Science & Technology GA DS1TZ UT WOS:000380384900004 PM 27455928 ER PT J AU Appell, M Mueller, A AF Appell, Michael Mueller, Anja TI Mycotoxin Analysis Using Imprinted Materials Technology: Recent Developments SO JOURNAL OF AOAC INTERNATIONAL LA English DT Article ID SOLID-PHASE EXTRACTION; ELECTROCHEMICAL SENSOR; LIQUID-CHROMATOGRAPHY; OCHRATOXIN-A; FLUORESCENCE DETECTION; SELECTIVE EXTRACTION; SAMPLE CLEANUP; HPLC ANALYSIS; T-2 TOXIN; POLYMERS AB Molecular imprinting technology is an attractive, cost-effective, and robust alternative to address the limitations of highly selective natural receptors, such as antibodies and aptamers. The field of molecular imprinting has seen a recent surge in growth, and several commercially available products are of great interest for sample cleanup to improve mycotoxin analysis. Current research trends are in specific applications of imprinting technology for small-molecule sensing and chromatographic cleanup procedures in new commodities. The choice of components and imprinting template are critical factors for mycotoxin recovery or detection optimization. Template mimics offer a means to reduce toxic exposure during polymer synthesis and address issues of leaching template from the imprinted polymer. Recent reports of molecularly imprinted polymers for aflatoxins, ochratoxins, fumonisins, fusaric acid, citrinin, patulin, zearalenone, deoxynivalenol, and T-2 toxin are reviewed. C1 [Appell, Michael] ARS, USDA, Natl Ctr Agr Utilizat Res, Mycotoxin Prevent & Appl Microbiol Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. [Mueller, Anja] Cent Michigan Univ, Chem & Biochem, Dow Sci Complex 374, Mt Pleasant, MI 48859 USA. RP Appell, M (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, Mycotoxin Prevent & Appl Microbiol Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM michael.appell@ars.usda.gov NR 56 TC 1 Z9 1 U1 21 U2 28 PU AOAC INT PI GAITHERSBURG PA 481 N FREDRICK AVE, STE 500, GAITHERSBURG, MD 20877-2504 USA SN 1060-3271 EI 1944-7922 J9 J AOAC INT JI J. AOAC Int. PD JUL-AUG PY 2016 VL 99 IS 4 BP 861 EP 864 DI 10.5740/jaoacint.16-0113 PG 4 WC Chemistry, Analytical; Food Science & Technology SC Chemistry; Food Science & Technology GA DS1TZ UT WOS:000380384900005 PM 27214609 ER PT J AU Kumar, SV Zaitchik, BF Peters-Lidard, CD Rodell, M Reichle, R Li, BL Jasinski, M Mocko, D Getirana, A De Lannoy, G Cosh, MH Hain, CR Anderson, M Arsenault, KR Xia, YL Ek, M AF Kumar, Sujay V. Zaitchik, Benjamin F. Peters-Lidard, Christa D. Rodell, Matthew Reichle, Rolf Li, Bailing Jasinski, Michael Mocko, David Getirana, Augusto De Lannoy, Gabrielle Cosh, Michael H. Hain, Christopher R. Anderson, Martha Arsenault, Kristi R. Xia, Youlong Ek, Michael TI Assimilation of Gridded GRACE Terrestrial Water Storage Estimates in the North American Land Data Assimilation System SO JOURNAL OF HYDROMETEOROLOGY LA English DT Article ID ENSEMBLE KALMAN FILTER; TIME-VARIABLE GRAVITY; SOIL-MOISTURE; SURFACE MODEL; ESTIMATING EVAPOTRANSPIRATION; CLIMATE EXPERIMENT; RIVER-BASIN; SNOW COVER; VARIABILITY; RETRIEVALS AB The objective of the North American Land Data Assimilation System (NLDAS) is to provide best-available estimates of near-surface meteorological conditions and soil hydrological status for the continental United States. To support the ongoing efforts to develop data assimilation (DA) capabilities for NLDAS, the results of Gravity Recovery and Climate Experiment (GRACE) DA implemented in a manner consistent with NLDAS development are presented. Following previous work, GRACE terrestrial water storage (TWS) anomaly estimates are assimilated into the NASA Catchment land surface model using an ensemble smoother. In contrast to many earlier GRACE DA studies, a gridded GRACE TWS product is assimilated, spatially distributed GRACE error estimates are accounted for, and the impact that GRACE scaling factors have on assimilation is evaluated. Comparisons with quality-controlled in situ observations indicate that GRACE DA has a positive impact on the simulation of unconfined groundwater variability across the majority of the eastern United States and on the simulation of surface and root zone soil moisture across the country. Smaller improvements are seen in the simulation of snow depth, and the impact of GRACE DA on simulated river discharge and evapotranspiration is regionally variable. The use of GRACE scaling factors during assimilation improved DA results in the western United States but led to small degradations in the eastern United States. The study also found comparable performance between the use of gridded and basin-averaged GRACE observations in assimilation. Finally, the evaluations presented in the paper indicate that GRACE DA can be helpful in improving the representation of droughts. C1 [Kumar, Sujay V.; Peters-Lidard, Christa D.; Rodell, Matthew; Li, Bailing; Jasinski, Michael; Mocko, David; Getirana, Augusto; Arsenault, Kristi R.] NASA, Goddard Space Flight Ctr, Hydrol Sci Lab, Code 617, Greenbelt, MD 20771 USA. [Zaitchik, Benjamin F.] Johns Hopkins Univ, Dept Earth & Planetary Sci, Baltimore, MD 21218 USA. [Reichle, Rolf; Mocko, David] NASA, Goddard Space Flight Ctr, Global Modeling & Assimilat Off, Greenbelt, MD USA. [Li, Bailing; Getirana, Augusto; Hain, Christopher R.] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20742 USA. [Mocko, David] Sci Applicat Int Corp, Beltsville, MD USA. [De Lannoy, Gabrielle] Univ Leuven, Dept Earth & Environm Sci, Leuven, Belgium. [Cosh, Michael H.; Anderson, Martha] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD USA. [Hain, Christopher R.] NOAA, Ctr Satellite Applicat & Res, College Pk, MD USA. [Arsenault, Kristi R.] Sci Applicat Int Corp, Mclean, VA 22102 USA. [Xia, Youlong] NCEP EMC, IMSG, College Pk, MD USA. [Xia, Youlong; Ek, Michael] Natl Ctr Environm Predict, Environm Modeling Ctr, College Pk, MD USA. RP Kumar, SV (reprint author), NASA, Goddard Space Flight Ctr, Hydrol Sci Lab, Code 617, Greenbelt, MD 20771 USA. EM sujay.v.kumar@nasa.gov RI Peters-Lidard, Christa/E-1429-2012; Reichle, Rolf/E-1419-2012; Rodell, Matthew/E-4946-2012; Kumar, Sujay/B-8142-2015; OI Peters-Lidard, Christa/0000-0003-1255-2876; Rodell, Matthew/0000-0003-0106-7437; Anderson, Martha/0000-0003-0748-5525 FU NASA Science Mission Directorate's Earth Science Division through the National Climate Assessment (NCA) project; NOAA's Climate Program Office MAPP program FX Funding for this work was provided by the NASA Science Mission Directorate's Earth Science Division through the National Climate Assessment (NCA) project and NOAA's Climate Program Office MAPP program. Computing was supported by the resources at the NASA Center for Climate Simulation. The NLDAS-2 forcing data used in this effort were acquired as part of the activities of NASA's Science Mission Directorate and are archived and distributed by the Goddard Earth Sciences (GES) Data and Information Services Center (DISC). The GRACE land data available at http://grace.jpl.nasa.gov were supported by the NASA MEaSUREs program. NR 55 TC 2 Z9 2 U1 11 U2 14 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 1525-755X EI 1525-7541 J9 J HYDROMETEOROL JI J. Hydrometeorol. PD JUL PY 2016 VL 17 IS 7 BP 1951 EP 1972 DI 10.1175/JHM-D-15-0157.1 PG 22 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DS1FV UT WOS:000380342100004 ER PT J AU Romano, FR Heinze, CR Barber, LG Mason, JB Freeman, LM AF Romano, F. R. Heinze, C. R. Barber, L. G. Mason, J. B. Freeman, L. M. TI Association between Body Condition Score and Cancer Prognosis in Dogs with Lymphoma and Osteosarcoma SO JOURNAL OF VETERINARY INTERNAL MEDICINE LA English DT Article DE Lymphoma; Nutrition; Obesity; Osteosarcoma ID MASS INDEX; INFERIOR SURVIVAL; HIGH-BMI; COLORECTAL-CANCER; HODGKINS-LYMPHOMA; PROSTATE-CANCER; HEART-FAILURE; WEIGHT-LOSS; OBESITY; IMPACT AB BackgroundIn humans and rodents obesity appears to promote some cancers by increasing incidence, tumor aggressiveness, recurrence, and fatality. However, the relationship between obesity and cancer in dogs has not been thoroughly evaluated. Hypothesis/ObjectivesWhether body condition score (BCS) at the time of lymphoma (LSA) or osteosarcoma (OSA) diagnosis in dogs is predictive of survival time (ST) or progression-free interval (PFI). We hypothesized that an overweight body state at the time of cancer diagnosis would be associated with negative outcomes. AnimalsDogs with LSA (n = 270) and OSA (n = 54) diagnosed and treated between 2000 and 2010. MethodsRetrospective case review. Signalment, body weight, BCS, cancer diagnosis and treatment, relevant clinicopathologic values, and survival data were collected. Dogs were grouped by BCS (underweight, ideal, and overweight) and ST and PFI were compared. ResultsOverall, 5.5% of dogs were underweight, 54.0% were ideal weight, and 40.4% were overweight at diagnosis. Underweight dogs with LSA had shorter ST (P = .017) than ideal or overweight dogs. BCS was not associated with ST for OSA (P = .474). Progression-free interval did not differ among BCS categories for either cancer. Conclusions and Clinical ImportanceObesity was not associated with adverse outcomes among dogs with LSA or OSA in this retrospective study; however, being underweight at the time of diagnosis of LSA was associated with shorter survival. More research is needed to elucidate the relationship between excessive body weight and cancer development and progression in dogs. C1 [Romano, F. R.; Heinze, C. R.; Barber, L. G.; Freeman, L. M.] Tufts Univ, Dept Clin Sci, Cummings Sch Vet Med, 200 Westboro Rd, North Grafton, MA 01536 USA. [Mason, J. B.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr, Boston, MA 02111 USA. RP Heinze, CR (reprint author), Tufts Univ, Dept Clin Sci, Cummings Sch Vet Med, 200 Westboro Rd, North Grafton, MA 01536 USA. EM cailin.heinze@tufts.edu FU Merial Veterinary Scholars program; National Center for Advancing Translational Sciences, National Institutes of Health [UL1TR000073]; U.S. Department of Agriculture [58-1950-7-707] FX Grant Support: This project was funded by the Merial Veterinary Scholars program. The project described was also supported in part by the National Center for Advancing Translational Sciences, National Institutes of Health, Award Number UL1TR000073, and the U.S. Department of Agriculture, under agreement No. 58-1950-7-707. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH, or do any opinions, findings, conclusion, or recommendations expressed in this publication necessarily reflect the view of the U.S. Dept. of Agriculture. NR 41 TC 0 Z9 0 U1 8 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0891-6640 EI 1939-1676 J9 J VET INTERN MED JI J. Vet. Intern. Med. PD JUL-AUG PY 2016 VL 30 IS 4 BP 1179 EP 1186 DI 10.1111/jvim.13965 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA DS3UQ UT WOS:000380708600031 PM 27279003 ER PT J AU Solana, M Qureshi, N Bertucco, A Eller, F AF Solana, Miriam Qureshi, Nasib Bertucco, Alberto Eller, Fred TI Recovery of Butanol by Counter-Current Carbon Dioxide Fractionation with its Potential Application to Butanol Fermentation SO MATERIALS LA English DT Article DE butanol; CO2 fractionation; separation efficiency; butanol rich extract; raffinate ID DILUTE AQUEOUS-SOLUTIONS; CLOSTRIDIUM-BEIJERINCKII BA101; N-BUTANOL; CORN STOVER; ETHANOL ABE; ACETOBUTYLICUM; ACETONE; PERVAPORATION; ADSORPTION; EXTRACTION AB A counter-current CO2 fractionation method was applied as a mean to recover n-butanol and other compounds that are typically obtained from biobutanol fermentation broth from aqueous solutions. The influence of operating variables, such as solvent-to-feed ratio, temperature, pressure and feed solution composition was experimentally studied in terms of separation efficiency, butanol removal rate, total removal and butanol concentration in the extract at the end of the continuous cycle. With respect to the temperature and pressure conditions investigated, results show that the highest separation efficiency was obtained at 35 degrees C and 10.34 MPa. At these operating conditions, 92.3% of the butanol present in the feed solution was extracted, and a concentration of 787.5 g.L-1 of butanol in the extract was obtained, starting from a feed solution of 20 g.L-1. Selectivity was calculated from experimental data, concluding that our column performs much better than a single equilibrium stage. When adding ethanol and acetone to the feed solution, ethanol was detected in the water-rich fraction (raffinate), whereas the highest concentration of acetone was found in the butanol rich fraction (extract). C1 [Solana, Miriam; Bertucco, Alberto] Univ Padua, Dept Ind Engn DII, Via Marzolo 9, I-35131 Padua, Italy. [Solana, Miriam; Eller, Fred] USDA, Natl Ctr Agr Utilizat Res, Funct Foods Res Unit, 1815 North Univ St, Peoria, IL 61604 USA. [Qureshi, Nasib] USDA, Natl Ctr Agr Utilizat Res, Bioenergy Res Unit, 1815 North Univ St, Peoria, IL 61604 USA. RP Solana, M (reprint author), Univ Padua, Dept Ind Engn DII, Via Marzolo 9, I-35131 Padua, Italy.; Solana, M (reprint author), USDA, Natl Ctr Agr Utilizat Res, Funct Foods Res Unit, 1815 North Univ St, Peoria, IL 61604 USA.; Qureshi, N (reprint author), USDA, Natl Ctr Agr Utilizat Res, Bioenergy Res Unit, 1815 North Univ St, Peoria, IL 61604 USA. EM miriam.solanacipres@studenti.unipd.it; Nasib.Qureshi@ars.usda.gov; alberto.bertucco@unipd.it; Fred.Eller@ARS.USDA.GOV FU Cassa di Risparmio del Veneto Foundation FX Miriam Solana gratefully acknowledges the doctoral scholarship for foreign students from Cassa di Risparmio del Veneto Foundation. Technical assistance was provided by Jeffrey Teel. NR 36 TC 0 Z9 0 U1 4 U2 4 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1996-1944 J9 MATERIALS JI Materials PD JUL PY 2016 VL 9 IS 7 AR 530 DI 10.3390/ma9070530 PG 11 WC Materials Science, Multidisciplinary SC Materials Science GA DS4OX UT WOS:000380761500032 ER PT J AU Wang, GF Balint-Kurti, PJ AF Wang, Guan-Feng Balint-Kurti, Peter J. TI Maize Homologs of CCoAOMT and HCT, Two Key Enzymes in Lignin Biosynthesis, Form Complexes with the NLR Rp1 Protein to Modulate the Defense Response SO PLANT PHYSIOLOGY LA English DT Article ID ASSOCIATION MAPPING POPULATION; ASSISTED GENE IDENTIFICATION; RUST RESISTANCE LOCUS; PROGRAMMED CELL-DEATH; SOUTHERN LEAF-BLIGHT; HYPERSENSITIVE RESPONSE; DISEASE-RESISTANCE; III EFFECTOR; PLANT-DISEASE; PHENYLPROPANOID METABOLISM AB Disease resistance (R) genes encode nucleotide binding Leu-rich-repeat (NLR) proteins that confer resistance to specific pathogens. Upon pathogen recognition they trigger a defense response that usually includes a so-called hypersensitive response (HR), a rapid localized cell death at the site of pathogen infection. Intragenic recombination between two maize (Zea mays) NLRs, Rp1-D and Rp1-dp2, resulted in the formation of a hybrid NLR, Rp1-D21, which confers an autoactive HR in the absence of pathogen infection. From a previous quantitative trait loci and genome-wide association study, we identified genes encoding two key enzymes in lignin biosynthesis, hydroxycinnamoyltransferase (HCT) and caffeoyl CoA O-methyltransferase (CCoAOMT), adjacent to the nucleotide polymorphisms that were highly associated with variation in the severity of Rp1-D21-induced HR. We have previously shown that the two maize HCT homologs suppress the HR conferred by Rp1-D21 in a heterologous system, very likely through physical interaction. Here, we show, similarly, that CCoAOMT2 suppresses the HR induced by either the full-length or by the N-terminal coiled-coil domain of Rp1-D21 also likely via physical interaction and that the metabolic activity of CCoAOMT2 is unlikely to be necessary for its role in suppressing HR. We also demonstrate that CCoAOMT2, HCTs, and Rp1 proteins can form in the same complexes. A model is derived to explain the roles of CCoAOMT and HCT in Rp1-mediated defense resistance. C1 [Wang, Guan-Feng; Balint-Kurti, Peter J.] North Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. [Wang, Guan-Feng] Shandong Univ, Sch Life Sci, Key Lab Plant Cell Engn & Germplasm Innovat, Minist Educ, Jinan 250100, Shandong, Peoples R China. [Balint-Kurti, Peter J.] ARS, USDA, Plant Sci Res Unit, Raleigh, NC 27695 USA. RP Wang, GF (reprint author), North Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA.; Wang, GF (reprint author), Shandong Univ, Sch Life Sci, Key Lab Plant Cell Engn & Germplasm Innovat, Minist Educ, Jinan 250100, Shandong, Peoples R China. EM gfwang@sdu.edu.cn OI Balint-Kurti, Peter/0000-0002-3916-194X FU National Science Foundation [0822495, 1444503]; U.S. Department of Agriculture-Agricultural Research Service; Qilu Scholarship from Shandong University of China [11200085963025] FX This work was supported by National Science Foundation (grant nos. 0822495 and 1444503), the U.S. Department of Agriculture-Agricultural Research Service, and Qilu Scholarship from Shandong University of China (grant no. 11200085963025 to G.-F.W.). NR 73 TC 2 Z9 2 U1 13 U2 17 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD JUL PY 2016 VL 171 IS 3 BP 2166 EP 2177 DI 10.1104/pp.16.00224 PG 12 WC Plant Sciences SC Plant Sciences GA DT2IF UT WOS:000381303300052 PM 27208251 ER PT J AU Longland, WS Dimitri, LA AF Longland, William S. Dimitri, Lindsay A. TI SEED SELECTION BY DESERT RODENTS: IMPLICATIONS FOR ENHANCING SEEDLING ESTABLISHMENT OF INDIAN RICEGRASS (ACHNATHERUM HYMENOIDES) SO WESTERN NORTH AMERICAN NATURALIST LA English DT Article ID MERRIAMS KANGAROO RATS; ORYZOPSIS-HYMENOIDES; DIPODOMYS-MERRIAMI; DIVERSIONARY FOOD; POPULATIONS; ENVIRONMENT; PREFERENCES; HYPOTHESES AB Seeds of many plant species are dispersed by seed-caching rodents that place groups of seeds in superficially buried scatterhoard caches. A case in point is Indian ricegrass (Achnatherum hymenoides), an important forage plant on arid western rangelands for which seedling recruitment comes largely from scatterhoards made by desert heteromyid rodents. A "diversionary seeding" strategy has been attempted for enhancing Indian ricegrass seedling recruitment by deploying commercially available seeds on the soil surface to divert rodents from recovering scatterhoards of Indian ricegrass seeds. The probability of such a passive restoration approach succeeding is likely affected by the relative desirability to rodents of Indian ricegrass seeds versus diversionary seeds. We conducted laboratory experiments to test preferences of Merriam's kangaroo rat (Dipodomys merriami), a primary dispersal agent of Indian ricegrass seeds, for Indian ricegrass seeds versus seeds of 5 diversionary seed candidates. Indian ricegrass seeds were consistently preferred over only 1 of the 5 alternate seeds in pairwise trials. In multiseed trials that presented all seed types (i.e., Indian ricegrass and the 5 alternates) simultaneously, ranking patterns of individual kangaroo rats varied significantly and Indian ricegrass was not preferred by any of the animals tested. Because individual kangaroo rats differed in seed preferences and all animals consumed certain seed types in greater amounts than they consumed Indian ricegrass, we suggest that using a mixture of different seed types in diversionary seedings is superior to deploying a single type of diversionary seeds. Understanding how population-level niche breadth is affected by dietary variation at the individual level can thus have important management implications. C1 [Longland, William S.] ARS, USDA, Great Basin Rangelands Res Unit, 920 Valley Rd, Reno, NV 89512 USA. [Dimitri, Lindsay A.] Univ Nevada, Dept Biol, Reno, NV 89557 USA. RP Longland, WS (reprint author), ARS, USDA, Great Basin Rangelands Res Unit, 920 Valley Rd, Reno, NV 89512 USA. EM bill.longland@ars.usda.gov NR 17 TC 0 Z9 0 U1 7 U2 7 PU BRIGHAM YOUNG UNIV PI PROVO PA 290 LIFE SCIENCE MUSEUM, PROVO, UT 84602 USA SN 1527-0904 EI 1944-8341 J9 WEST N AM NATURALIST JI West. North Am. Naturalist PD JUL PY 2016 VL 76 IS 2 BP 253 EP 258 PG 6 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DS6GH UT WOS:000380879700006 ER PT J AU Mensink, RP Sanders, TA Baer, DJ Hayes, KC Howles, PN Marangoni, A AF Mensink, Ronald P. Sanders, Thomas A. Baer, David J. Hayes, K. C. Howles, Philip N. Marangoni, Alejandro TI The Increasing Use of Interesterified Lipids in the Food Supply and Their Effects on Health Parameters SO ADVANCES IN NUTRITION LA English DT Review DE interesterified fats; dietary intake; metabolism; health effects; trans fatty acids ID FATTY-ACID-COMPOSITION; PLASMA-LIPIDS; FACTOR-VII; SN-2 POSITION; PALMITIC ACID; STRUCTURED TRIACYLGLYCEROLS; POSTPRANDIAL LIPEMIA; LYMPHATIC ABSORPTION; LINOLEIC-ACID; CHAIN TRIGLYCERIDES AB A variety of modified fats that provide different functionalities are used in processed foods to optimize product characteristics and nutrient composition. Partial hydrogenation results in the formation of trans FAs (TFAs) and was one of the most widely used modification processes of fats and oils. However, the negative effects of commercially produced TFAs on serum lipoproteins and risk for cardiovascular disease resulted in the Institute of Medicine and the 2010 US Dietary Guidelines for Americans both recommending that TFA intake be as low as possible. After its tentative 2013 determination that use of partially hydrogenated oils is not generally regarded as safe, the FDA released its final determination of the same in 2015. Many food technologists have turned to interesterified fat as a replacement. Interesterification rearranges FAs within and between a triglyceride molecule by use of either a chemical catalyst or an enzyme. Although there is clear utility of interesterified fats for retaining functional properties of food, the nutrition and health implications of long-term interesterified fat consumption are less well understood. The Technical Committee on Dietary Lipids of the North American Branch of the International Life Sciences Institute sponsored a workshop to discuss the health effects of interesterified fats, identify research needs, and outline considerations for the design of future studies. The consensus was that although interesterified fat production is a feasible and economically viable solution for replacing dietary TFAs, outstanding questions must be answered regarding the effects of interesterification on modifying certain aspects of lipid and glucose metabolism, inflammatory responses, hemostatic parameters, and satiety. C1 [Mensink, Ronald P.] Maastricht Univ, Med Ctr, Maastricht, Netherlands. [Sanders, Thomas A.] Kings Coll London, London, England. [Baer, David J.] ARS, USDA, Beltsville, MD USA. [Hayes, K. C.] Brandeis Univ, Waltham, MA USA. [Howles, Philip N.] Univ Cincinnati, Coll Med, Cincinnati, OH USA. [Marangoni, Alejandro] Univ Guelph, Guelph, ON, Canada. RP Mensink, RP (reprint author), Maastricht Univ, Med Ctr, Maastricht, Netherlands. EM r.mensink@maastrichtuniversity.nl FU ILSI North America Technical Committee on Dietary Lipids FX As Senior Science Program Manager at ILSI North America at the time of this work, P Courtney Gaine contributed significantly to this manuscript by managing the development and editorial stages of this article following the workshop held in 2012. Science Writer Denise Webb contributed to the development of early drafts of this article and received funds from the ILSI North America Technical Committee on Dietary Lipids. All authors read and approved the final version of the paper and take responsibility for all aspects of the paper. NR 64 TC 1 Z9 1 U1 11 U2 17 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 2161-8313 EI 2156-5376 J9 ADV NUTR JI Adv. Nutr. PD JUL PY 2016 VL 7 IS 4 BP 719 EP 729 DI 10.3945/an.115.009662 PG 11 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DS1FU UT WOS:000380342000008 PM 27422506 ER PT J AU Dwyer, JT Rubin, KH Fritsche, KL Psota, TL Liska, DJ Harris, WS Montain, SJ Lyle, BJ AF Dwyer, Johanna T. Rubin, Kristin H. Fritsche, Kevin L. Psota, Tricia L. Liska, Deann J. Harris, William S. Montain, Scott J. Lyle, Barbara J. TI Creating the Future of Evidence-Based Nutrition Recommendations: Case Studies from Lipid Research SO ADVANCES IN NUTRITION LA English DT Article DE dietary guidelines; fatty acids; lipid metabolism; public policy; systematic review ID CORONARY-HEART-DISEASE; C-REACTIVE PROTEIN; TRANS-FATTY-ACIDS; PLACEBO-CONTROLLED TRIAL; CARDIOVASCULAR-DISEASE; MYOCARDIAL-INFARCTION; SATURATED FAT; RISK-FACTORS; INFLAMMATION; BIOMARKERS AB Strategic translational research is designed to address research gaps that answer specific guidance questions. It provides translational value with respect to nutrition guidance and regulatory and public policy. The relevance and the quality of evidence both matter in translational research. For example, design decisions regarding population, intervention, comparator, and outcome criteria affect whether or not high-quality studies are considered relevant to specific guidance questions and are therefore included as evidence within the context of systematic review frameworks used by authoritative food and health organizations. The process used in systematic reviews, developed by the USDA for its Nutrition Evidence Library, is described. An eating pattern and cardiovascular disease (CVD) evidence review is provided as an example, and factors that differentiated the studies considered relevant and included in that evidence base from those that were excluded are noted. Case studies on omega-3 (n-3) fatty acids (FAs) and industrial trans-FAs illustrate key factors vital to relevance and translational impact, including choice of a relevant population (e.g., healthy, at risk, or diseased subjects; general population or high-performance soldiers); dose and form of the intervention (e.g., food or supplement); use of relevant comparators (e.g., technically feasible and realistic); and measures for both exposure and outcomes (e.g., inflammatory markers or CVD endpoints). Specific recommendations are provided to help increase the impact of nutrition research on future dietary guidance, policy, and regulatory issues, particularly in the area of lipids. C1 [Dwyer, Johanna T.] Tufts Univ, Sch Med, Tufts Med Ctr, Boston, MA 02111 USA. [Dwyer, Johanna T.] Tufts Univ, Sch Nutr Sci & Policy, Tufts Med Ctr, Boston, MA 02111 USA. [Dwyer, Johanna T.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Rubin, Kristin H.] Kraft Heinz Co, Glenview, IL USA. [Fritsche, Kevin L.] Univ Missouri, Columbia, MO USA. [Psota, Tricia L.] USDA, Ctr Nutr & Policy Promot, Alexandria, VA USA. [Liska, Deann J.] Biofortis Res, Addison, IL USA. [Harris, William S.] OmegaQuant Analyt LLC, Sioux Falls, SD USA. [Harris, William S.] Univ South Dakota, Sch Med, Sioux Falls, SD USA. [Montain, Scott J.] US Army, Environm Med Res Inst, Natick, MA 01760 USA. [Lyle, Barbara J.] Northwestern Univ, Sch Profess Studies, Evanston, IL 60208 USA. [Lyle, Barbara J.] Int Life Sci Inst, North Amer Branch, Washington, DC 20005 USA. RP Lyle, BJ (reprint author), Northwestern Univ, Sch Profess Studies, Evanston, IL 60208 USA.; Lyle, BJ (reprint author), Int Life Sci Inst, North Amer Branch, Washington, DC 20005 USA. EM lyleinc@att.net OI Lyle, Barbara/0000-0002-0362-7736; Fritsche, Kevin/0000-0002-0740-3801; Harris, William/0000-0003-3042-9353 FU American Society for Nutrition (ASN); International Life Sciences Institute (ILSI) North America; Technical Committee on Dietary Lipids of the North American Branch of the ILSI FX This article is a review from the symposium "Creating the Future of Evidence-Based Nutrition Recommendations, Using Lipid Research Case Studies" held 28 March 2015 at the ASN Scientific Sessions and Annual Meeting at Experimental Biology 2015 in Boston, MA. The symposium was sponsored by the American Society for Nutrition (ASN) and supported and sponsored by the International Life Sciences Institute (ILSI) North America. This is a free access article, distributed under terms (http://www.nutrition.org/publications/guidelinesand-policies/license/) that permit unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.; Supported by the Technical Committee on Dietary Lipids of the North American Branch of the ILSI. Detailed information about the group can be found at http://www.ilsi.org/NorthAmerica/Pages/DietaryLipids.aspx. ILSI North America is a public, nonprofit foundation that provides a forum to advance understanding of scientific issues related to the nutritional quality and safety of the food supply by sponsoring research programs, educational seminars and workshops, and publications. ILSI North America receives financial support primarily from its industry membership. NR 57 TC 1 Z9 1 U1 6 U2 7 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 2161-8313 EI 2156-5376 J9 ADV NUTR JI Adv. Nutr. PD JUL PY 2016 VL 7 IS 4 BP 747 EP 755 DI 10.3945/an.115.010926 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DS1FU UT WOS:000380342000011 PM 27422509 ER PT J AU Scott, TM AF Scott, Tammy M. TI Comment on "Perspective: A Critical Look at the Ancillary Age-Related Eye Disease Study 2: Nutrition and Cognitive Function Results in Older Individuals with Age-Related Macular Degeneration" SO ADVANCES IN NUTRITION LA English DT Letter ID TELEPHONE C1 [Scott, Tammy M.] Tufts Univ, Sch Med, Jean Mayer USDA Human Nutr Ctr Aging, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Scott, Tammy M.] Tufts Med Ctr, Boston, MA 02111 USA. RP Scott, TM (reprint author), Tufts Univ, Sch Med, Jean Mayer USDA Human Nutr Ctr Aging, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA.; Scott, TM (reprint author), Tufts Med Ctr, Boston, MA 02111 USA. EM tammy.scott@tufts.edu NR 5 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 2161-8313 EI 2156-5376 J9 ADV NUTR JI Adv. Nutr. PD JUL PY 2016 VL 7 IS 4 BP 756 EP 756 DI 10.3945/an.116.012690 PG 1 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DS1FU UT WOS:000380342000012 PM 27422510 ER PT J AU Blumberg, JB Basu, A Krueger, CG Lila, MA Neto, CC Novotny, JA Reed, JD Rodriguez-Mateos, A Toner, CD AF Blumberg, Jeffrey B. Basu, Arpita Krueger, Christian G. Lila, Mary Ann Neto, Catherine C. Novotny, Janet A. Reed, Jess D. Rodriguez-Mateos, Ana Toner, Cheryl D. TI Impact of Cranberries on Gut Microbiota and Cardiometabolic Health: Proceedings of the Cranberry Health Research Conference 2015 SO ADVANCES IN NUTRITION LA English DT Article; Proceedings Paper CT Cranberry Health Research Conference CY OCT 12, 2015 CL Madison, WI SP Cranberry Inst, US Cranberry Mkt Comm, Amer Cranberry Growers Assoc DE cranberry; proanthocyanidins; microbiome; cardiometabolic; antimicrobial ID UROPATHOGENIC ESCHERICHIA-COLI; ELEMENTAL ENTERAL NUTRITION; PLASMA ANTIOXIDANT CAPACITY; PLACEBO-CONTROLLED TRIAL; URINARY-TRACT-INFECTION; DIET-INDUCED OBESITY; C-REACTIVE PROTEIN; HIGH-FAT DIET; JUICE CONSUMPTION; MASS-SPECTROMETRY AB Recent advances in cranberry research have expanded the evidence for the role of this Vaccinium berry fruit in modulating gut microbiota function and cardiometabolic risk factors. The A-type structure of cranberry proanthocyanidins seems to be responsible for much of this fruit's efficacy as a natural antimicrobial. Cranberry proanthocyanidins interfere with colonization of the gut by extraintestinal pathogenic Escherichia coli in vitro and attenuate gut barrier dysfunction caused by dietary insults in vivo. Furthermore, new studies indicate synergy between these proanthocyanidins, other cranberry components such as isoprenoids and xyloglucans, and gut microbiota. Together, cranberry constituents and their bioactive catabolites have been found to contribute to mechanisms affecting bacterial adhesion, coaggregation, and biofilm formation that may underlie potential clinical benefits on gastrointestinal and urinary tract infections, as well as on systemic anti-inflammatory actions mediated via the gut microbiome. A limited but growing body of evidence from randomized clinical trials reveals favorable effects of cranberry consumption on measures of cardiometabolic health, including serum lipid profiles, blood pressure, endothelial function, glucoregulation, and a variety of biomarkers of inflammation and oxidative stress. These results warrant further research, particularly studies dedicated to the elucidation of dose-response relations, pharmacokinetic/metabolomics profiles, and relevant biomarkers of action with the use of fully characterized cranberry products. Freeze-dried whole cranberry powder and a matched placebo were recently made available to investigators to facilitate such work, including interlaboratory comparability. C1 [Blumberg, Jeffrey B.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Basu, Arpita] Oklahoma State Univ, Stillwater, OK 74078 USA. [Krueger, Christian G.; Reed, Jess D.] Complete Phytochem Solut LLC, Cambridge, WI USA. [Krueger, Christian G.; Reed, Jess D.] Univ Wisconsin, Madison, WI USA. [Lila, Mary Ann] North Carolina State Univ, Kannapolis, NC USA. [Neto, Catherine C.] Univ Massachusetts Dartmouth, Dartmouth, MA USA. [Novotny, Janet A.] USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. [Rodriguez-Mateos, Ana] Univ Dusseldorf, Dusseldorf, Germany. [Toner, Cheryl D.] Cranberry Inst, Carver, MA USA. [Toner, Cheryl D.] CDT Consulting LLC, Herndon, VA USA. RP Blumberg, JB (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM jeffrey.blumberg@tufts.edu OI Rodriguez-Mateos, Ana/0000-0003-3242-402X NR 115 TC 4 Z9 4 U1 17 U2 19 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 2161-8313 EI 2156-5376 J9 ADV NUTR JI Adv. Nutr. PD JUL PY 2016 VL 7 IS 4 BP 759S EP 770S DI 10.3945/an.116.012583 PG 12 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DS1FU UT WOS:000380342000013 PM 27422512 ER PT J AU Parisien, MA Miller, C Parks, SA DeLancey, ER Robinne, FN Flannigan, MD AF Parisien, Marc-Andre Miller, Carol Parks, Sean A. DeLancey, Evan R. Robinne, Francois-Nicolas Flannigan, Mike D. TI The spatially varying influence of humans on fire probability in North America SO Environmental Research Letters LA English DT Article DE wildfire; North America; anthropogenic influence; climate; topography; lightning ID WESTERN UNITED-STATES; BOREAL WILDFIRES; CLIMATE-CHANGE; FOREST-FIRES; REGIMES; VEGETATION; PATTERNS; HISTORY; CANADA; SUPPRESSION AB Humans affect fire regimes by providing ignition sources in some cases, suppressing wildfires in others, and altering natural vegetation in ways that may either promote or limit fire. In North America, several studies have evaluated the effects of society on fire activity; however, most studies have been regional or subcontinental in scope and used different data and methods, there by making continent-wide comparisons difficult. We circumvent these challenges by investigating the broad-scale impact of humans on fire activity using parallel statistical models of fire probability from 1984 to 2014 as a function of climate, enduring features (topography and percent nonfuel), lightning, and three indices of human activity (population density, an integrated metric of human activity [Human Footprint Index], and a measure of remoteness [roadless volume]) across equally spaced regions of the United States and Canada. Through a statistical control approach, where by we account for the effect of other explanatory variables, we found evidence of non-negligible human-wildfire association across the entire continent, even in the most sparsely populated areas. A surprisingly coherent negative relationship between fire activity and humans was observed across the United States and Canada: fire probability generally diminishes with increasing human influence. Intriguing exceptions to this relationship are the continent's least disturbed areas, where fewer humans equate to less fire. These remote areas, however, also often have lower lightning densities, leading us to believe that they may be ignition limited at the spatiotemporal scale of the study. Our results suggest that there are few purely natural fire regimes in North America today. Consequently, projections of future fire activity should consider human impacts on fire regimes to ensure sound adaptation and mitigation measures in fire-prone areas. C1 [Parisien, Marc-Andre; DeLancey, Evan R.] Nat Resources Canada, Canadian Forest Serv, Northern Forestry Ctr, 5320-122nd St, Edmonton, AB T6H 3S5, Canada. [Miller, Carol; Parks, Sean A.] US Forest Serv, Aldo Leopold Wilderness Res Inst, Rocky Mt Res Stn, USDA, 790 E Beckwith Ave, Missoula, MT 59801 USA. [DeLancey, Evan R.; Robinne, Francois-Nicolas; Flannigan, Mike D.] Univ Alberta, Western Partnership Wildland Fire Sci, Dept Renewable Resources, 751 Gen Serv Bldg, Edmonton, AB T6G 2H1, Canada. RP Parisien, MA (reprint author), Nat Resources Canada, Canadian Forest Serv, Northern Forestry Ctr, 5320-122nd St, Edmonton, AB T6H 3S5, Canada. EM marc-andre.parisien@canada.ca FU Adaptation Program of Natural Resources Canada FX This study was partially funded by the Adaptation Program of Natural Resources Canada. We are grateful to Nicolas Mansuy and two anonymous reviewers for constructive comments. NR 83 TC 5 Z9 5 U1 13 U2 18 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD JUL PY 2016 VL 11 IS 7 AR 075005 DI 10.1088/1748-9326/11/7/075005 PG 18 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DS5JD UT WOS:000380817000034 ER PT J AU Whittier, J Sievert, N Loftus, A Defilippi, JM Krogman, RM Ojala, J Litts, T Kopaska, J Eiden, N AF Whittier, Joanna Sievert, Nick Loftus, Andrew Defilippi, Julie M. Krogman, Rebecca M. Ojala, Jeffrey Litts, Thom Kopaska, Jeff Eiden, Nicole TI Leveraging BIG Data from BIG Databases to Answer BIG Questions SO FISHERIES LA English DT Article C1 [Whittier, Joanna] Univ Missouri, Dept Fisheries & Wildlife Sci, 302 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. [Sievert, Nick] Missouri Cooperat Fish & Wildlife Res Unit, Columbia, MO USA. [Loftus, Andrew] Loftus Consulting, Annapolis, MD USA. [Defilippi, Julie M.] Atlantic Coastal Cooperat Stat Program, Arlington, VA USA. [Krogman, Rebecca M.] Iowa Dept Nat Resources, Chariton, IA USA. [Ojala, Jeffrey] US Forest Serv, Logan, UT USA. [Litts, Thom] Georgia Dept Nat Resources Fisheries, Social Circle, GA USA. [Kopaska, Jeff] Iowa Dept Nat Resources, Boone, IA USA. [Eiden, Nicole] Arizona Game & Fish Dept, Phoenix, AZ USA. RP Whittier, J (reprint author), Univ Missouri, Dept Fisheries & Wildlife Sci, 302 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. EM whittierj@missouri.edu OI Sievert, Nicholas/0000-0003-3160-7596 NR 11 TC 0 Z9 0 U1 1 U2 1 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0363-2415 EI 1548-8446 J9 FISHERIES JI Fisheries PD JUL PY 2016 VL 41 IS 7 SI SI BP 417 EP 419 DI 10.1080/03632415.2016.1191911 PG 3 WC Fisheries SC Fisheries GA DR8KY UT WOS:000380148000018 ER PT J AU Huang, LH AF Huang, Lihan TI Evaluating the Performance of a New Model for Predicting the Growth of Clostridium perfringens in Cooked, Uncured Meat and Poultry Products under Isothermal, Heating, and Dynamically Cooling Conditions SO JOURNAL OF FOOD SCIENCE LA English DT Article DE C; perfringens; cooling evaluation; predictive modeling ID ORGANIC-ACID SALTS; GROUND-BEEF; SPORE GERMINATION; ROAST BEEF; TURKEY BREAST; OREGANO OIL; OUTGROWTH; INHIBITION; PORK; CINNAMALDEHYDE AB Clostridium perfringens type A is a significant public health threat and its spores may germinate, outgrow, and multiply during cooling of cooked meats. This study applies a new C. perfringens growth model in the USDA Integrated Pathogen Modeling Program-Dynamic Prediction (IPMP Dynamic Prediction) Dynamic Prediction to predict the growth from spores of C. perfringens in cooked uncured meat and poultry products using isothermal, dynamic heating, and cooling data reported in the literature. The residual errors of predictions (observation-prediction) are analyzed, and the root-mean-square error (RMSE) calculated. For isothermal and heating profiles, each data point in growth curves is compared. The mean residual errors (MRE) of predictions range from -0.40 to 0.02 Log colony forming units (CFU)/g, with a RMSE of approximately 0.6 Log CFU/g. For cooling, the end point predictions are conservative in nature, with an MRE of -1.16 Log CFU/g for single-rate cooling and -0.66 Log CFU/g for dual-rate cooling. The RMSE is between 0.6 and 0.7 Log CFU/g. Compared with other models reported in the literature, this model makes more accurate and fail-safe predictions. For cooling, the percentage for accurate and fail-safe predictions is between 97.6% and 100%. Under criterion 1, the percentage of accurate predictions is 47.5% for single-rate cooling and 66.7% for dual-rate cooling, while the fail-dangerous predictions are between 0% and 2.4%. This study demonstrates that IPMP Dynamic Prediction can be used by food processors and regulatory agencies as a tool to predict the growth of C. perfringens in uncured cooked meats and evaluate the safety of cooked or heat-treated uncured meat and poultry products exposed to cooling deviations or to develop customized cooling schedules. This study also demonstrates the need for more accurate data collection during cooling. Practical ApplicationClostridium perfringens is a significant public health hazard, and can be found in many meat and poultry products regulated by the USDA Food Safety and Inspection Service. A predictive model is needed for the food industry and regulatory agencies to estimate the relative growth of C. perfringens in the event of cooling deviation. The predictive model in IPMP Dynamic Prediction provides significant higher accurate and fail-safe predictions. Its use can reduce foodborne infections caused by the growth of C. perfringens in meat and poultry products during cooling. C1 [Huang, Lihan] ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. RP Huang, LH (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM lihan.huang@ars.usda.gov NR 35 TC 1 Z9 1 U1 6 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD JUL PY 2016 VL 81 IS 7 BP M1754 EP M1765 DI 10.1111/1750-3841.13356 PG 12 WC Food Science & Technology SC Food Science & Technology GA DR8NI UT WOS:000380154200020 PM 27259065 ER PT J AU Niu, Y Qureshi, JA Ni, XZ Head, GP Price, PA Meagher, RL Kerns, D Levy, R Yang, XB Huang, FN AF Niu, Ying Qureshi, Jawwad A. Ni, Xinzhi Head, Graham P. Price, Paula A. Meagher, Robert L., Jr. Kerns, David Levy, Ronnie Yang, Xiangbing Huang, Fangneng TI F-2 screen for resistance to Bacillus thuringiensis Cry2Ab2-maize in field populations of Spodoptera frugiperda (Lepidoptera: Noctuidae) from the southern United States SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Spodoptera frugiperda; Transgenic maize; Bacillus thuringiensis; Cry2Ab2; F-2 screening; Resistance management ID HELICOVERPA-ARMIGERA LEPIDOPTERA; ALLELES CONFERRING RESISTANCE; PYRAMIDED BT GENES; SUGARCANE BORER; FALL ARMYWORM; PUERTO-RICO; AUSTRALIAN POPULATIONS; INSECTICIDAL PROTEINS; EVOLVED RESISTANCE; CROSS-RESISTANCE AB The fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), is a target pest of transgenic maize and cotton expressing Bacillus thuringiensis (Bt) proteins in both North and South America. In 2013 and 2014, a total of 215 F-2 two-parent families of S. frugiperda were established using single-pair mating of field individuals collected from seven locations in four states of the southern U.S.: Texas, Louisiana, Georgia, and Florida. The objective of the investigation was to detect resistance alleles in field populations to Cry2Ab2, a common Bt protein produced in transgenic maize and cotton. For each F-2 family, 128 F-2 neonates were screened on leaf tissue of Cry2Ab2 maize plants in the laboratory. A conservative estimate of the frequency of major Cry2Ab2 resistance alleles in S. frugiperda from the four states was 0.0023 with a 95% credibility interval of 0.0003-0.0064. In addition, six families were considered to likely possess minor resistance alleles at a frequency of 0.0082 with a 95% credibility interval of 0.0033-0.0152. One F-2 family from Georgia (GA-15) was confirmed to possess a major resistance allele to the Cry2Ab2 protein. Larvae from this family survived well on whole maize plants expressing Cry2Ab2 protein and demonstrated a significant level (>15-fold) of resistance when fed with the same protein incorporated in a meridic diet. The detection of the major resistance allele along with the relatively abundant minor resistance alleles revealed in this study may have important implications for resistance management. (C) 2016 Elsevier Inc. All rights reserved. C1 [Niu, Ying; Kerns, David; Huang, Fangneng] Louisiana State Univ, Ctr Agr, Dept Entomol, Baton Rouge, LA 70803 USA. [Qureshi, Jawwad A.] Univ Florida, Dept Entomol & Nematol, IFAS SWFREC, Immokalee, FL 34142 USA. [Ni, Xinzhi] USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. [Head, Graham P.; Price, Paula A.] Monsanto Co, St Louis, MO 63167 USA. [Meagher, Robert L., Jr.] USDA ARS, Behav & Biocontrol Unit, Gainesville, FL 32608 USA. [Levy, Ronnie] Louisiana State Univ, Ctr Agr, Dean Lee Res Stn, Alexandria, LA 71302 USA. [Yang, Xiangbing] Texas A&M AgriLife Res & Extens Ctr, Weslaco, TX 78596 USA. [Yang, Xiangbing] Univ Calif Davis, 1636 East Alisal St, Salinas, CA 93905 USA. RP Huang, FN (reprint author), Louisiana State Univ, Ctr Agr, Dept Entomol, Baton Rouge, LA 70803 USA. EM fhuang@agcenter.lsu.edu FU Monsanto Company; Louisiana Soybean and Feed Grain Promotion Board; USDA National Institute of Food and Agriculture FX This article is published with the approval of the Director of the Louisiana Agricultural Experiment Station as manuscript No. 2016-234-25967. This project represents work supported by Monsanto Company and the Louisiana Soybean and Feed Grain Promotion Board, and Hatch funds from the USDA National Institute of Food and Agriculture. NR 36 TC 0 Z9 0 U1 8 U2 8 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 EI 1096-0805 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD JUL PY 2016 VL 138 BP 66 EP 72 DI 10.1016/j.jip.2016.06.005 PG 7 WC Zoology SC Zoology GA DS1UA UT WOS:000380385000010 PM 27311896 ER PT J AU Minatel, IO Lee, YM Yoon, H Yoon, Y Han, SI Correa, CR Fecchio, D Yeum, KJ AF Minatel, Igor Otavio Lee, Yoon-Mi Yoon, Haelim Yoon, Young Han, Sang-Ik Correa, Camila Renata Fecchio, Denise Yeum, Kyung-Jin TI Antiadipogenic Activity of gamma-Oryzanol and Its Stability in Pigmented Rice SO JOURNAL OF MEDICINAL FOOD LA English DT Article DE adipogenesis; bioactive compound; gamma-oryzanol; mesenchymal stem cells; obesity ID CARDIOVASCULAR-DISEASE; WHITE RICE; FERULIC ACID; COLORED RICE; BROWN RICE; DIET; QUANTITATION; VARIETIES; QUALITY; ESTER AB gamma-Oryzanol, a prevalent compound in pigmented rice varieties, has been reported to ameliorate obesity-associated metabolic disorders. Antiadipogenic activities of gamma-oryzanol were determined in human adipose-derived mesenchymal stem cells and mouse-derived 3T3-L1 cells. gamma-Oryzanol significantly decreased lipid accumulation and reduced glycerol-3-phosphate dehydrogenase activities in both adipocytes. In addition, gamma-oryzanol in four pigmented rice varieties (black with giant embryo, brown, sugary brown, and red) was stable when stored at 4 degrees C and also at room temperature for 22 weeks, whereas other bioactives such as lutein and beta-carotene were stable only at -80 degrees C. Furthermore, the yield of gamma-oryzanol from these rice varieties was significantly increased through steaming and roasting processes. Therefore, gamma-oryzanol exerts antiadipogenic activity by suppressing adipocyte differentiations and is stable in pigmented rice for an extended period of time during storage and after cooking. Thus, the intake of pigmented rice may be a useful strategy for preventing obesity. C1 [Minatel, Igor Otavio] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Minatel, Igor Otavio; Correa, Camila Renata; Fecchio, Denise] UNESP Univ Estadual Paulista, Botucatu Med Sch, Botucatu, SP, Brazil. [Lee, Yoon-Mi; Yoon, Haelim; Yoon, Young; Yeum, Kyung-Jin] Konkuk Univ, Coll Biomed & Hlth Sci, Div Food Biosci, Chungju Si 380701, Chungcheongbuk, South Korea. [Lee, Yoon-Mi; Yoon, Haelim; Yoon, Young; Yeum, Kyung-Jin] Konkuk Univ, Interdisciplinary Res Ctr Hlth, Chungju Si, Chungcheongbuk, South Korea. [Han, Sang-Ik] Rural Dev Adm, Natl Inst Crop Sci, Miryang Si, Gyeongsangnam D, South Korea. RP Yeum, KJ (reprint author), Konkuk Univ, Coll Biomed & Hlth Sci, Div Food Biosci, Chungju Si 380701, Chungcheongbuk, South Korea. EM kyeum@kku.ac.kr FU Rural Development Administration, Republic of Korea [PJ010059] FX Supported in part by Rural Development Administration (PJ010059), Republic of Korea. NR 30 TC 0 Z9 0 U1 5 U2 6 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1096-620X EI 1557-7600 J9 J MED FOOD JI J. Med. Food PD JUL PY 2016 VL 19 IS 7 BP 710 EP 715 DI 10.1089/jmf.2015.3647 PG 6 WC Chemistry, Medicinal; Food Science & Technology; Nutrition & Dietetics SC Pharmacology & Pharmacy; Food Science & Technology; Nutrition & Dietetics GA DS1ZY UT WOS:000380511400012 PM 27309274 ER PT J AU Arruda, MP Lipka, AE Brown, PJ Krill, AM Thurber, C Brown-Guedira, G Dong, Y Foresman, BJ Kolb, FL AF Arruda, M. P. Lipka, A. E. Brown, P. J. Krill, A. M. Thurber, C. Brown-Guedira, G. Dong, Y. Foresman, B. J. Kolb, F. L. TI Comparing genomic selection and marker-assisted selection for Fusarium head blight resistance in wheat (Triticum aestivum L.) SO MOLECULAR BREEDING LA English DT Article DE Molecular breeding; Genotyping-by-sequencing; Fusarium graminearum; Marker-assisted selection; Genomic selection; Deoxynivalenol ID WIDE ASSOCIATION; GENETIC ARCHITECTURE; PREDICTION MODELS; QTL; ACCURACY; METAANALYSIS; RELATEDNESS; SET AB Genomic selection (GS) and marker-assisted selection (MAS) rely on marker-trait associations and are both routinely used for breeding purposes. Although similar, these two approaches differ in their applications and how markers are used to estimate breeding values. In this study, GS and MAS were compared in their ability to predict six traits associated with resistance to a destructive wheat disease, Fusarium head blight (FHB). A panel consisting in 273 soft red winter wheat lines from the US Midwestern and Eastern regions was used in this study. The statistical models for MAS were built using Fhb-1, the best-studied quantitative trait loci (QTL) for FHB resistance, and two sets of QTL: one independently identified by other groups and a newer set identified "in house". In contrast, genomic selection models relied on 19,992 SNPs distributed throughout the genome. For the MAS and GS models, marker effects were estimated with ordinary least square and ridge regression best unbiased linear prediction, respectively. Intermediate to high values of prediction accuracy (0.4-0.9) were observed for most GS models, with lower values (<0.3) found for MAS models. Treating QTL as fixed effects in GS models resulted in higher prediction accuracy when compared with a GS model with only random effects, but overestimated accuracies were obtained with in house QTL. For the same selection intensity, GS resulted in higher selection differentials than MAS for all traits. Our results indicate that GS is a more appropriate strategy than MAS for FHB resistance. C1 [Arruda, M. P.; Lipka, A. E.; Brown, P. J.; Krill, A. M.; Foresman, B. J.; Kolb, F. L.] Univ Illinois, Dept Crop Sci, 1102 South Goodwin, Urbana, IL 61801 USA. [Thurber, C.] Abraham Baldwin Agr Coll, Sch Sci & Math, 2802 Moore Highway, Tifton, GA USA. [Brown-Guedira, G.] ARS, USDA, Eastern Reg Small Grains Genotyping Lab, 4114 Williams Hall, Raleigh, NC USA. [Dong, Y.] Univ Minnesota, Dept Plant Pathol, 1991 Upper Buford Circle, St Paul, MN USA. RP Kolb, FL (reprint author), Univ Illinois, Dept Crop Sci, 1102 South Goodwin, Urbana, IL 61801 USA. EM f-kolb@illinois.edu FU U.S. Department of Agriculture [59-0206-4-029]; Monsanto Company FX This material is based upon work supported by the U.S. Department of Agriculture, under Agreement No. 59-0206-4-029. This is a cooperative project with the U.S. Wheat & Barley Scab Initiative. The first author is grateful to Monsanto Company for providing graduate studies fellowship. NR 49 TC 2 Z9 2 U1 13 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD JUL PY 2016 VL 36 IS 7 AR 84 DI 10.1007/s11032-016-0508-5 PG 11 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DR9TF UT WOS:000380238300002 ER PT J AU Hwang, S King, CA Chen, PY Ray, JD Cregan, PB Carter, TE Li, ZL Abdel-Haleem, H Matson, KW Schapaugh, W Purcell, LC AF Hwang, Sadal King, C. Andy Chen, Pengyin Ray, Jeffery D. Cregan, Perry B. Carter, Thomas E., Jr. Li, Zenglu Abdel-Haleem, Hussein Matson, Kevin W. Schapaugh, William, Jr. Purcell, Larry C. TI Meta-analysis to refine map position and reduce confidence intervals for delayed-canopy-wilting QTLs in soybean SO MOLECULAR BREEDING LA English DT Article DE Drought stress; Meta-analysis; QTL; Soybean; Wilting ID QUANTITATIVE TRAIT LOCI; RECOMBINANT INBRED LINES; GLYCINE-MAX; L.-MERR.; AGRONOMIC TRAITS; FLOODING TOLERANCE; DROUGHT TOLERANCE; CYST-NEMATODE; GENETIC-MAP; YIELD AB Slow canopy wilting in soybean has been identified as a potentially beneficial trait for ameliorating drought effects on yield. Previous research identified QTLs for slow wilting from two different biparental populations, and this information was combined with data from three other populations to identify nine QTL clusters for slow wilting on Gm02, Gm05, Gm11, Gm 14, Gm17, and Gm19. The QTL cluster on Gm14 was eliminated because these QTLs appeared to be false positives. In the present research, QTLs from these remaining eight clusters were compiled onto the soybean consensus map for metaQTL analysis. Five model selection criteria were used to determine the most appropriate number of metaQTLs at these eight chromosomal regions. For a QTL cluster on Gm02, two meta-QTLs were identified, whereas for the remaining seven QTL clusters the single meta-QTL model was most appropriate. Thus, the analysis identified nine meta-QTLs associated with slow wilting. Meta-analysis decreased the confidence intervals from an average of 21.4 cM for the eight QTL clusters to 10.8 cM for the meta-QTLs. Averaged R-2 values of the nine meta-QTLs in eight QTL clusters were 0.13 and ranged from 0.09 to 0.22. Meta-QTLs on Gm11 and Gm19 had the highest R-2 values (0.22 and 0.20, respectively). C1 [Hwang, Sadal; King, C. Andy; Chen, Pengyin; Purcell, Larry C.] Univ Arkansas, Dept Crop Soil & Environm Sci, 1336 Altheimer Dr, Fayetteville, AR 72704 USA. [Ray, Jeffery D.] ARS, Crop Genet & Prod Res Unit, USDA, Stoneville, MS 38776 USA. [Cregan, Perry B.] USDA, Soybean Genom & Improvement Lab, ARR, BARC West, Beltsville, MD 20705 USA. [Carter, Thomas E., Jr.] North Carolina State Univ, Dept Crop Sci, USDA, ARS, Raleigh, NC 27695 USA. [Li, Zenglu] Univ Georgia, Inst Plant Breeding Genet & Genom, Ctr Appl Genet Technol, 111 Riverbend Rd, Athens, GA 30602 USA. [Abdel-Haleem, Hussein] ARS, US Arid Land Agr Res Ctr, USDA, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. [Matson, Kevin W.] Monsanto Co, Global Soybean Breeding, St Louis, MO 63167 USA. [Schapaugh, William, Jr.] Kansas State Univ, Dept Agron, 2004C Throckmorton Hall, Manhattan, KS USA. RP Purcell, LC (reprint author), Univ Arkansas, Dept Crop Soil & Environm Sci, 1336 Altheimer Dr, Fayetteville, AR 72704 USA. EM lpurcell@uark.edu FU United Soybean Board; United States Department of Agriculture-Agriculture Research Service (USDA-ARS) project [6066-21220-012-00D] FX The authors gratefully acknowledge financial support for this research from the United Soybean Board. This work was also supported in part by the United States Department of Agriculture-Agriculture Research Service (USDA-ARS) project number 6066-21220-012-00D. NR 80 TC 0 Z9 0 U1 6 U2 6 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD JUL PY 2016 VL 36 IS 7 AR 91 DI 10.1007/s11032-016-0516-5 PG 14 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DR9TF UT WOS:000380238300009 ER PT J AU Ma, Y Hu, JG Myers, JR Mazourek, M Coyne, CJ Main, D Wang, MN Humann, J McGee, RJ AF Ma, Yu Hu, Jinguo Myers, James R. Mazourek, Michael Coyne, Clarice J. Main, Dorrie Wang, Meinan Humann, Jodi McGee, Rebecca J. TI Development of SCAR markers linked to sin-2, the stringless pod trait in pea (Pisum sativum L.) SO MOLECULAR BREEDING LA English DT Article DE Pisum sativum; Stringless; Pod suture; TRAP marker; SCAR marker ID HELIANTHUS-ANNUUS L.; DOMESTICATION SYNDROME; POLLEN TUBES; GENE; RESISTANCE; IDENTIFICATION; AMPLIFICATION; POLYMORPHISM; GENERATION; WHEAT AB With increasing consumer demand for vegetables, edible-podded peas have become more popular. Stringlessness is one of most important traits for snap peas. A single recessive gene, sin-2, controls this trait. Because pollen carrying the stringless gene is less competitive than pollen carrying the stringy gene, there are fewer than expected stringless plants recovered in segregating generations. Marker-assisted selection (MAS) is a valuable tool to identify plants with the traits of interest at an early stage in the breeding process. The objective of this study was to identify robust, user-friendly molecular markers tightly linked to sin-2. A total of 144 target region amplification polymorphism (TRAP) primer combinations were used to screen four DNA bulks, which were constructed from 32 pea breeding lines based on their phenotypes. Sixty polymorphic TRAP primer combinations were identified between bulks of stringless and stringy pods. Five primer combinations, F6_Trap03_168, F6_SA12_145, F10_ODD8_130, F11_GA5_850, and F12_SA12_190, showed more than 90 % association with the stringless phenotype in 32 pea breeding lines. Two of the TRAP markers, F10_ODD8_130 and F12_SA12_190, were cloned, sequenced, and successfully converted to sequence characterized amplified region (SCAR) markers. These two SCAR markers were validated using 20 F5 recombinant inbred lines derived from a cross between Bohatyr (a dry pea variety with strings) and S1188 (a stringless snap pea variety) and showed strong marker-trait association. The results will have direct application in MAS of stringless edible-podded peas. C1 [Ma, Yu; Main, Dorrie; Humann, Jodi] Washington State Univ, Dept Hort, Pullman, WA 99164 USA. [Hu, Jinguo; Coyne, Clarice J.] ARS, Plant Germplasm Intro & Testing, USDA, Pullman, WA 99164 USA. [Myers, James R.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Mazourek, Michael] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14850 USA. [Wang, Meinan] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [McGee, Rebecca J.] ARS, Grain Legume Genet & Physiol Res Unit, USDA, Pullman, WA 99164 USA. RP McGee, RJ (reprint author), ARS, Grain Legume Genet & Physiol Res Unit, USDA, Pullman, WA 99164 USA. EM rjmcgee@wsu.edu FU USDA-ARS CRIS Projects [2090-21000-028-00D, 2090-21000-024-00D]; Washington State University FX The authors gratefully acknowledge the funding sources: USDA-ARS CRIS Projects #2090-21000-028-00D (JH, CJC) and 2090-21000-024-00D (RJM). Liangsheng Xu and Lisa Taylor are greatly acknowledged for their technical assistance. Also, thanks for the support from Washington State University. NR 37 TC 0 Z9 0 U1 5 U2 5 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD JUL PY 2016 VL 36 IS 7 AR 105 DI 10.1007/s11032-016-0525-4 PG 10 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DR9TF UT WOS:000380238300023 ER PT J AU Zhang, XF Rouse, MN Nava, IC Jin, Y Anderson, JA AF Zhang, Xiaofei Rouse, Matthew N. Nava, Itamar C. Jin, Yue Anderson, James A. TI Development and verification of wheat germplasm containing both Sr2 and Fhb1 SO MOLECULAR BREEDING LA English DT Article DE Fusarium head blight; Stem rust; Sr2; Fhb1; Wheat ID FUSARIUM-HEAD-BLIGHT; TRITICUM-AESTIVUM L.; RUST RESISTANCE GENE; MARKER-ASSISTED SELECTION; PSEUDO-BLACK CHAFF; STEM RUST; SPRING WHEAT; MAJOR QTL; WINTER-WHEAT; BARLEY AB Fusarium head blight (FHB) and stem rust are two devastating fungal diseases of common wheat prevalent worldwide. Fhb1 is a well-known major quantitative trait locus effective against FHB, and Sr2 is the most widely characterized and deployed wheat stem rust adult plant resistance gene. These genes are linked in repulsion phase, approximately 3 cM apart, on chromosome 3B. To obtain wheat germplasm containing both Fhb1 and Sr2 genes, we developed and screened two F-2 populations using tightly linked DNA markers, Xgwm533 and csSr2 for Sr2, and UMN10, and a newly designed marker UMN10(v2) for Fhb1. Among 1949 F-2 plants, 43 were identified with Fhb1 and Sr2 in coupling phase. After screened with vernalization gene markers, 59 F-3 spring wheat lines with homozygous Fhb1 and Sr2 were obtained. All these lines showed significantly lower FHB severity than susceptible checks (P < 0.01) and similar FHB severity to the resistant checks (P > 0.61) in the field. And these lines were susceptible as seedlings in greenhouse assays, but resistant as adult plants to race QTHJC in the stem rust nursery. Field disease resistance evaluation verified that all these recombinant lines showed moderate or high levels of resistance to both FHB and stem rust. This germplasm would benefit efforts to breed for resistance to FHB and stem rust, and the procedure to obtain recombinants is adaptable to other wheat breeding programs. C1 [Zhang, Xiaofei; Nava, Itamar C.; Anderson, James A.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Rouse, Matthew N.; Jin, Yue] Univ Minnesota, Cereal Dis Lab, ARS, USDA, St Paul, MN 55108 USA. [Rouse, Matthew N.; Jin, Yue] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Nava, Itamar C.] Fed Univ Rio Grande Sul UFRGS, Dept Crop Sci, Ave Bento Goncalves 7712,POB 15100, BR-91501970 Porto Alegre, RS, Brazil. RP Anderson, JA (reprint author), Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. EM ander319@umn.edu RI Rouse, Matthew/G-5474-2011 FU United States Department of Agriculture-Agricultural Research Service US Wheat and Barley Scab Initiative, Durable Rust Resistance in Wheat project (Cornell University) FX This work was supported by grants from the United States Department of Agriculture-Agricultural Research Service US Wheat and Barley Scab Initiative, Durable Rust Resistance in Wheat project (Cornell University). The authors thank Dr. Scott Haley (Colorado State University, Fort Collins, CO) for his permission to use CO02W280 and CO03W269 as donors of Sr2 and as parents in our crosses. NR 51 TC 0 Z9 0 U1 2 U2 2 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD JUL PY 2016 VL 36 IS 7 AR 85 DI 10.1007/s11032-016-0502-y PG 14 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DR9TF UT WOS:000380238300003 ER PT J AU Chibucos, MC Soliman, S Gebremariam, T Lee, H Daugherty, S Orvis, J Shetty, AC Crabtree, J Hazen, TH Etienne, KA Kumari, P O'Connor, TD Rasko, DA Filler, SG Fraser, CM Lockhart, SR Skory, CD Ibrahim, AS Bruno, VM AF Chibucos, Marcus C. Soliman, Sameh Gebremariam, Teclegiorgis Lee, Hongkyu Daugherty, Sean Orvis, Joshua Shetty, Amol C. Crabtree, Jonathan Hazen, Tracy H. Etienne, Kizee A. Kumari, Priti O'Connor, Timothy D. Rasko, David A. Filler, Scott G. Fraser, Claire M. Lockhart, Shawn R. Skory, Christopher D. Ibrahim, Ashraf S. Bruno, Vincent M. TI An integrated genomic and transcriptomic survey of mucormycosis-causing fungi SO Nature Communications LA English DT Article ID MAXIMUM-LIKELIHOOD PHYLOGENIES; CANDIDA-ALBICANS INFECTION; RHIZOPUS-ORYZAE; PROINFLAMMATORY RESPONSE; TRANSPLANT RECIPIENTS; SEQUENCE ALIGNMENTS; ENDOTHELIAL-CELLS; ZYGOMYCOSIS; PHOSPHORYLATION; EPIDEMIOLOGY AB Mucormycosis is a life-threatening infection caused by Mucorales fungi. Here we sequence 30 fungal genomes, and perform transcriptomics with three representative Rhizopus and Mucor strains and with human airway epithelial cells during fungal invasion, to reveal key host and fungal determinants contributing to pathogenesis. Analysis of the host transcriptional response to Mucorales reveals platelet-derived growth factor receptor B (PDGFRB) signaling as part of a core response to divergent pathogenic fungi; inhibition of PDGFRB reduces Mucorales-induced damage to host cells. The unique presence of CotH invasins in all invasive Mucorales, and the correlation between CotH gene copy number and clinical prevalence, are consistent with an important role for these proteins in mucormycosis pathogenesis. Our work provides insight into the evolution of this medically and economically important group of fungi, and identifies several molecular pathways that might be exploited as potential therapeutic targets. C1 [Chibucos, Marcus C.; Hazen, Tracy H.; Rasko, David A.; Bruno, Vincent M.] Univ Maryland, Sch Med, Dept Microbiol & Immunol, Baltimore, MD 21201 USA. [Chibucos, Marcus C.; Daugherty, Sean; Orvis, Joshua; Shetty, Amol C.; Crabtree, Jonathan; Hazen, Tracy H.; Kumari, Priti; O'Connor, Timothy D.; Rasko, David A.; Fraser, Claire M.; Bruno, Vincent M.] Univ Maryland, Sch Med, Inst Genome Sci, Baltimore, MD 21201 USA. [Soliman, Sameh; Gebremariam, Teclegiorgis; Lee, Hongkyu; Filler, Scott G.; Ibrahim, Ashraf S.] Harbor UCLA Med Ctr, Div Infect Dis, Los Angeles Biomed Res Inst, Torrance, CA 90502 USA. [Etienne, Kizee A.; Lockhart, Shawn R.] Ctr Dis Control & Prevent, Fungal Reference Lab, Mycot Dis Branch, Atlanta, GA 30333 USA. [O'Connor, Timothy D.] Univ Maryland, Sch Med, Dept Med, Baltimore, MD 21201 USA. [Filler, Scott G.; Ibrahim, Ashraf S.] Univ Calif Los Angeles, David Geffen Sch Med, Los Angeles, CA 90502 USA. [Skory, Christopher D.] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Soliman, Sameh] Univ Sharjah, Dept Med Chem, Coll Pharm, POB 27272, Sharjah, U Arab Emirates. RP Bruno, VM (reprint author), Univ Maryland, Sch Med, Dept Microbiol & Immunol, Baltimore, MD 21201 USA.; Bruno, VM (reprint author), Univ Maryland, Sch Med, Inst Genome Sci, Baltimore, MD 21201 USA.; Ibrahim, AS (reprint author), Harbor UCLA Med Ctr, Div Infect Dis, Los Angeles Biomed Res Inst, Torrance, CA 90502 USA.; Ibrahim, AS (reprint author), Univ Calif Los Angeles, David Geffen Sch Med, Los Angeles, CA 90502 USA. EM ibrahim@labiomed.org; vbruno@som.umaryland.edu OI Chibucos, Marcus/0000-0001-9586-0780 FU National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Department of Health and Human Services [HHSN272200900009C]; [U19AI110820]; [R01AI054928]; [R01DE017088]; [R01 AI063503]; [R41 AI115907] FX This project was funded in part with federal funds from the National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Department of Health and Human Services under contract number HHSN272200900009C. V.M.B., D.A.R., C.M.F., S.G.F. and A.S.I. were supported by U19AI110820. S.G.F. was supported by grants R01AI054928 and R01DE017088. A.S.I. was supported by grants R01 AI063503 and R41 AI115907. The endothelial cells used in these studies were isolated from human umbilical cords, which were collected by the paediatric, perinatal and mobile unit of the UCLA Clinical and Translational Science Institute at LA BioMed/Harbor-UCLA Medical Center (UL1TR000124). NR 75 TC 2 Z9 2 U1 10 U2 13 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD JUL PY 2016 VL 7 AR 12218 DI 10.1038/ncomms12218 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DS5RF UT WOS:000380839300001 PM 27447865 ER PT J AU Nelson-Kaula, KK Ostertag, R Hughes, RF Dudley, BD AF Nelson-Kaula, Kehauwealani K. Ostertag, Rebecca Hughes, R. Flint Dudley, Bruce D. TI Nutrient and Organic Matter Inputs to Hawaiian Anchialine Ponds: Influences of N-Fixing and Non-N-Fixing Trees SO PACIFIC SCIENCE LA English DT Article ID BIOLOGICAL INVASION; THESPESIA-POPULNEA; NITROGEN-FIXATION; PROSOPIS-PALLIDA; N-2-FIXING TREE; MYRICA-FAYA; FOOD WEBS; LITTER; DYNAMICS; PATTERNS AB Invasive nitrogen-fixing plants often increase energy and nutrient inputs to both terrestrial and aquatic ecosystems via litterfall, and these effects may be more pronounced in areas lacking native N-2-fixers. We examined organic matter and nutrient inputs to and around anchialine ponds on Hawai'i Island's leeward coast from an invasive, N-2-fixing tree, Prosopis pallida, and a non-N-2-fixing tree, Thespesia populnea. On a monthly basis we quantified: (1) tree basal area and stem density surrounding the ponds, and (2) nutrient content and quantity of P. pallida and T. populnea litter inputs. Tree density and basal area significantly predicted litterfall mass inputs for both species, but there was no difference in mean total annual litterfall mass, foliar N concentration, or litterfall N between the pond types. Time did not significantly influence litterfall mass, C, or P but did affect litterfall N, likely due to the production of P. pallida pods. In contrast, P concentrations were three times greater in T. populnea litter than in P. pallida litter, resulting in significantly higher P deposition where T. populnea was the prevalent tree species. Because this deposition of labile P by the non-N-2-fixing T. populnea has the potential to strongly influence water quality and soil chemistry, its replacement by P. pallida in tree communities of lowland Hawai'i may alter the functioning of anchialine pond ecosystems. C1 [Nelson-Kaula, Kehauwealani K.; Ostertag, Rebecca; Dudley, Bruce D.] Univ Hawaii, Dept Biol & Trop Conservat Biol & Environm Sci, Hilo, HI 96720 USA. [Hughes, R. Flint; Dudley, Bruce D.] USDA ARS, Inst Pacific Isl Forestry, Hilo, HI 96720 USA. RP Nelson-Kaula, KK (reprint author), Univ Hawaii, Dept Biol & Trop Conservat Biol & Environm Sci, Hilo, HI 96720 USA. EM kehauwealani@gmail.com FU National Science Foundation (Hawai'i EPSCoR NSF) [EPS-0903833] FX Funding was provided by the National Science Foundation (Hawai'i EPSCoR NSF Grant No. EPS-0903833), with additional logistical support provided by the U.S. Forest Service Institute of Pacific Islands Forestry. NR 67 TC 1 Z9 1 U1 3 U2 4 PU UNIV HAWAII PRESS PI HONOLULU PA 2840 KOLOWALU ST, HONOLULU, HI 96822 USA SN 0030-8870 EI 1534-6188 J9 PAC SCI JI Pac. Sci. PD JUL PY 2016 VL 70 IS 3 BP 333 EP 347 DI 10.2984/70.3.5 PG 15 WC Marine & Freshwater Biology; Zoology SC Marine & Freshwater Biology; Zoology GA DS1OJ UT WOS:000380365500006 ER PT J AU Flack, KD Davy, BM DeBerardinis, M Boutagy, NE McMillan, RP Hulver, MW Frisard, MI Anderson, AS Savla, J Davy, KP AF Flack, Kyle D. Davy, Brenda M. DeBerardinis, Martin Boutagy, Nabil E. McMillan, Ryan P. Hulver, Matthew W. Frisard, Madlyn I. Anderson, Angela S. Savla, Jyoti Davy, Kevin P. TI Resistance exercise training and invitro skeletal muscle oxidative capacity in older adults SO PHYSIOLOGICAL REPORTS LA English DT Article DE Metabolism; mitochondria; oxidative damage; strength ID SARCOPENIA; METABOLISM; HYPERTROPHY; ADAPTATION; HUMANS; MEN; DNA AB Whether resistance exercise training (RET) improves skeletal muscle substrate oxidative capacity and reduces mitochondrial production of reactive oxygen species in older adults remains unclear. To address this, 19 older males (60years) were randomized to a RET (n=11) or to a waitlist control group (n=8) that remained sedentary for 12weeks. RET was comprised of three upper body and four lower body movements on resistance machines. One set of 8-12 repetitions to failure of each movement was performed on three nonconsecutive days/week. Improvements in chest press and leg press strength were assessed using a three-repetition maximum (3 RM). Body composition was assessed via dual energy X-ray absorptiometry. Muscle biopsies were obtained from the vastus lateralis muscle at baseline and at both 3weeks and 12weeks. Palmitate and pyruvate oxidation rates were measured from the (CO2)-C-14 produced from [1-C-14] palmitic acid and [U-C-14] pyruvate, respectively, during incubation of muscle homogenates. PGC-1, TFAM, and PPAR levels were quantified using qRT-PCR. Citrate synthase (CS) and -HAD activities were determined spectrophotometrically. Mitochondrial production of reactive oxygen species (ROS) were assessed using the Amplex Red Hydrogen Peroxide/Peroxidase assay. There were no significant changes in body weight or body composition following the intervention. Chest press and leg press strength (3RM) increased similar to 34% (both P<0.01) with RET. There were no significant changes in pyruvate or fatty acid oxidation or in the expression of target genes with the intervention. There was a modest increase (P<0.05) in HAD activity with RET at 12weeks but the change in CS enzyme activity was not significant. In addition, there were no significant changes in ROS production in either group following RET. Taken together, the findings of this study suggest that 12weeks of low volume RET does not increase skeletal muscle oxidative capacity or reduce ROS production in older adults. C1 [Flack, Kyle D.] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND USA. [Davy, Brenda M.; DeBerardinis, Martin; McMillan, Ryan P.; Hulver, Matthew W.; Frisard, Madlyn I.; Davy, Kevin P.] Virginia Tech, Dept Human Nutr Foods & Exercise, Blacksburg, VA USA. [Davy, Brenda M.; McMillan, Ryan P.; Hulver, Matthew W.; Frisard, Madlyn I.; Savla, Jyoti; Davy, Kevin P.] Virginia Tech, Fralin Translat Obes Res Ctr, Blacksburg, VA USA. [Davy, Brenda M.; Hulver, Matthew W.; Frisard, Madlyn I.; Savla, Jyoti; Davy, Kevin P.] Virginia Tech, Ctr Gerontol, Blacksburg, VA USA. [Boutagy, Nabil E.] Yale Univ, Sch Med, Dept Internal Med, Sect Cardiovasc Med, New Haven, CT 06510 USA. [McMillan, Ryan P.; Hulver, Matthew W.; Frisard, Madlyn I.; Davy, Kevin P.] Virginia Tech, Metab Phenotyping Core, Blacksburg, VA USA. [Anderson, Angela S.] Pikes Peak Community Coll, Dept Biol, Colorado Springs, CO USA. [Savla, Jyoti] Virginia Tech, Dept Human Dev, Blacksburg, VA USA. RP Flack, KD (reprint author), Dept Human Nutr Foods & Exercise, 215 War Mem Hall 0430,370 Drillfield Dr, Blacksburg, VA 24061 USA. EM kdavy@vt.edu RI Savla, Jyoti/J-7125-2015 OI Savla, Jyoti/0000-0001-7142-3770 NR 29 TC 0 Z9 0 U1 9 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2051-817X J9 PHYSIOL REP JI PHYSIOL. REP. PD JUL PY 2016 VL 4 IS 13 AR e12849 DI 10.14814/phy2.12849 PG 8 WC Physiology SC Physiology GA DS0AO UT WOS:000380257700003 ER PT J AU Altpeter, F Springer, NM Bartley, LE Blechl, AE Brutnell, TP Citovsky, V Conrad, LJ Gelvin, SB Jackson, DP Kausch, AP Lemaux, PG Medford, JI Orozco-Cardenas, ML Tricoli, DM Van Eck, J Voytas, DF Walbot, V Wang, K Zhang, ZYJ Stewart, CN AF Altpeter, Fredy Springer, Nathan M. Bartley, Laura E. Blechl, Ann E. Brutnell, Thomas P. Citovsky, Vitaly Conrad, Liza J. Gelvin, Stanton B. Jackson, David P. Kausch, Albert P. Lemaux, Peggy G. Medford, June I. Orozco-Cardenas, Martha L. Tricoli, David M. Van Eck, Joyce Voytas, Daniel F. Walbot, Virginia Wang, Kan Zhang, Zhanyuan J. Stewart, C. Neal, Jr. TI Advancing Crop Transformation in the Era of Genome Editing SO PLANT CELL LA English DT Article ID AGROBACTERIUM-MEDIATED TRANSFORMATION; PLANT SYNTHETIC BIOLOGY; GENETIC-TRANSFORMATION; T-DNA; TRANSCRIPTION FACTOR; VIRD2 PROTEIN; SOMATIC EMBRYOGENESIS; PARTICLE BOMBARDMENT; TRANSGENIC PLANTS; TUMEFACIENS VIRD2 AB Plant transformation has enabled fundamental insights into plant biology and revolutionized commercial agriculture. Unfortunately, for most crops, transformation and regeneration remain arduous even after more than 30 years of technological advances. Genome editing provides novel opportunities to enhance crop productivity but relies on genetic transformation and plant regeneration, which are bottlenecks in the process. Here, we review the state of plant transformation and point to innovations needed to enable genome editing in crops. Plant tissue culture methods need optimization and simplification for efficiency and minimization of time in culture. Currently, specialized facilities exist for crop transformation. Single-cell and robotic techniques should be developed for high-throughput genomic screens. Plant genes involved in developmental reprogramming, wound response, and/or homologous recombination should be used to boost the recovery of transformed plants. Engineering universal Agrobacterium tumefaciens strains and recruiting other microbes, such as Ensifer or Rhizobium, could facilitate delivery of DNA and proteins into plant cells. Synthetic biology should be employed for de novo design of transformation systems. Genome editing is a potential game-changer in crop genetics when plant transformation systems are optimized. C1 [Altpeter, Fredy] Univ Florida, IFAS, Dept Agron, Plant Mol & Cellular Biol Program, Gainesville, FL 32611 USA. [Springer, Nathan M.] Univ Minnesota, Dept Plant Biol, Microbial & Plant Genom Inst, St Paul, MN 55108 USA. [Bartley, Laura E.] Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK 73019 USA. [Blechl, Ann E.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. [Brutnell, Thomas P.] Donald Danforth Plant Sci Ctr, Enterprise Inst Renewable Fuels, St Louis, MO 63132 USA. [Citovsky, Vitaly] SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY 11794 USA. [Conrad, Liza J.] Eckerd Coll, Nat Sci Collegium, St Petersburg, FL 33711 USA. [Gelvin, Stanton B.] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. [Jackson, David P.] Cold Spring Harbor Lab, POB 100, Cold Spring Harbor, NY 11724 USA. [Kausch, Albert P.] Univ Rhode Isl, Dept Cellular & Mol Biol, Kingston, RI 02881 USA. [Lemaux, Peggy G.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Medford, June I.] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Orozco-Cardenas, Martha L.] Univ Calif Riverside, Plant Transformat Res Ctr, Riverside, CA 92521 USA. [Tricoli, David M.] Univ Calif Davis, Plant Transformat Facil, Davis, CA 95616 USA. [Van Eck, Joyce] Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Voytas, Daniel F.] Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA. [Voytas, Daniel F.] Univ Minnesota, Ctr Genome Engn, Minneapolis, MN 55455 USA. [Walbot, Virginia] Stanford Univ, Dept Biol, Stanford, CA 94305 USA. [Wang, Kan] Iowa State Univ, Inst Plant Sci, Dept Agron, Ames, IA 50011 USA. [Wang, Kan] Iowa State Univ, Inst Plant Sci, Ctr Plant Transformat, Ames, IA 50011 USA. [Zhang, Zhanyuan J.] Univ Missouri, Div Plant Sci, Plant Transformat Core Facil, Columbia, MO 65211 USA. [Stewart, C. Neal, Jr.] Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA. RP Stewart, CN (reprint author), Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA. EM nealstewart@utk.edu RI Brutnell, Thomas/M-2840-2013 OI Brutnell, Thomas/0000-0002-3581-8211 FU National Science Foundation-Plant Genome [IOS 1546708] FX We thank the National Science Foundation-Plant Genome (Grant IOS 1546708) for financial support of the workshop "Transformation enabled genomic research in crop plants" that resulted in this article. We thank the following scientists who contributed to important discussion during this workshop but are not listed as authors: Jim Birchler, Doane Chilcoat, Tom Clemente, Marceline Egnin, Bill Gordon-Kamm, Sarah Hake, Heidi Kaeppler, Patricia Klein, David Lee, Peggy Ozias-Akins, Ron Qu, Qiudeng Que, David Somers, David Songstad, Vibha Srivastava, Keerti Rathore, Jianping Wang, Zeng-Yu Wang, and Yinong Yang. We also thank Raechelle Gretencord for assistance in workshop organization and Barbara Alonso, Joshua Wong, Judith Owiti, Kangmei Zhao, and Jennifer Hinds for assistance in figure and manuscript preparation. We recognize Vladmir Orbovic and Guo-qing Song for contributions to Supplemental Table 1 and for discussions of public transformation facility status. NR 94 TC 8 Z9 9 U1 51 U2 60 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD JUL PY 2016 VL 28 IS 7 BP 1510 EP 1520 DI 10.1105/tpc.16.00196 PG 11 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA DS3OC UT WOS:000380691400004 PM 27335450 ER PT J AU Jiao, YP Burke, J Chopra, R Burow, G Chen, JP Wang, B Hayes, C Emendack, Y Ware, D Xin, ZG AF Jiao, Yinping Burke, John Chopra, Ratan Burow, Gloria Chen, Junping Wang, Bo Hayes, Chad Emendack, Yves Ware, Doreen Xin, Zhanguo TI A Sorghum Mutant Resource as an Efficient Platform for Gene Discovery in Grasses SO PLANT CELL LA English DT Article ID SINGLE NUCLEOTIDE POLYMORPHISMS; GENOME SEQUENCING REVEALS; BICOLOR L. MOENCH; CAENORHABDITIS-ELEGANS; INDUCED MUTATIONS; PROTEIN FUNCTION; OPEN SOFTWARE; MAIZE; POPULATION; ALIGNMENT AB Sorghum (Sorghum bicolor) is a versatile C4 crop and a model for research in family Poaceae. High-quality genome sequence is available for the elite inbred line BTx623, but functional validation of genes remains challenging due to the limited genomic and germplasm resources available for comprehensive analysis of induced mutations. In this study, we generated 6400 pedigreed M4 mutant pools from EMS-mutagenized BTx623 seeds through single-seed descent. Whole-genome sequencing of 256 phenotyped mutant lines revealed >1.8 million canonical EMS-induced mutations, affecting >95% of genes in the sorghum genome. The vast majority (97.5%) of the induced mutations were distinct from natural variations. To demonstrate the utility of the sequenced sorghum mutant resource, we performed reverse genetics to identify eight genes potentially affecting drought tolerance, three of which had allelic mutations and two of which exhibited exact cosegregation with the phenotype of interest. Our results establish that a large-scale resource of sequenced pedigreed mutants provides an efficient platform for functional validation of genes in sorghum, thereby accelerating sorghum breeding. Moreover, findings made in sorghum could be readily translated to other members of the Poaceae via integrated genomics approaches. C1 [Jiao, Yinping; Burke, John; Chopra, Ratan; Burow, Gloria; Chen, Junping; Hayes, Chad; Emendack, Yves; Xin, Zhanguo] USDA ARS, Plant Stress & Germplasm Dev Unit, Cropping Syst Res Lab, Lubbock, TX 79415 USA. [Jiao, Yinping; Wang, Bo; Ware, Doreen] Cold Spring Harbor Lab, POB 100, Cold Spring Harbor, NY 11724 USA. [Ware, Doreen] Cornell Univ, USDA ARS, NEA Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Xin, ZG (reprint author), USDA ARS, Plant Stress & Germplasm Dev Unit, Cropping Syst Res Lab, Lubbock, TX 79415 USA.; Ware, D (reprint author), Cold Spring Harbor Lab, POB 100, Cold Spring Harbor, NY 11724 USA.; Ware, D (reprint author), Cornell Univ, USDA ARS, NEA Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM ware@cshl.edu; zhanguo.xin@ars.usda.gov OI CHOPRA, RATAN/0000-0003-2088-3341 FU ARS in-house project [6208-21000-020-00D]; United Sorghum Checkoff Program; National Science Foundation [IOS 1127112]; USDA-ARS [1907-21000-030-00D] FX We thank Lan Liu-Gitz and Halee Hughes (USDA-ARS) for technical support and Veronica Acosta-Martinez (USDA-ARS) for technical assistance with GC analysis of waxes. The project was funded by ARS in-house project 6208-21000-020-00D and the United Sorghum Checkoff Program. We also acknowledge the funding from National Science Foundation IOS 1127112 (Y.J. and B.W.) and USDA-ARS 1907-21000-030-00D (D.W.) for data analysis. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. NR 69 TC 2 Z9 2 U1 3 U2 7 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD JUL PY 2016 VL 28 IS 7 BP 1551 EP 1562 DI 10.1105/tpc.16.00373 PG 12 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA DS3OC UT WOS:000380691400007 PM 27354556 ER PT J AU Avula, B Sagi, S Wang, YH Wang, M Gafner, S Manthey, JA Khan, IA AF Avula, Bharathi Sagi, Satyanarayanaraju Wang, Yan-Hong Wang, Mei Gafner, Stefan Manthey, John A. Khan, Ikhlas A. TI Liquid Chromatography-Electrospray Ionization Mass Spectrometry Analysis of Limonoids and Flavonoids in Seeds of Grapefruits, Other Citrus Species, and Dietary Supplements SO PLANTA MEDICA LA English DT Article DE Citrus samples; limonoid aglycones; limonoid glucosides; flavonoids; UHPLC-DAD-QToF-MS; preservatives; dietary supplements ID PHYSIOLOGICALLY ACTIVE SUBSTANCES; BENZETHONIUM CHLORIDE; ACIDIC LIMONOIDS; FRUIT TISSUES; GLUCOSIDES; FLAVANONES; EXTRACT; IDENTIFICATION; GLYCOSIDES; CULTIVARS AB A selective UHPLC-DAD-QToF-MS method was developed to screen grapefruit seeds, and the seeds of other Citrus species for limonoid aglycones, acids, glucosides, and flavonoids. These classes of compounds were identified in positive and negative ion modes over a mass-to-charge range from 100-1500. Accurate mass values, elution times, and fragmentation patterns obtained by QToF-mass spectrometry were used to identify or tentatively characterize the compounds detected in the sample of this study. Limonin was the major limonoid in most of the seeds of Citrus species, followed by nomilin. This analytical method was successfully applied for the analysis of commercial extracts and dietary supplements claiming to contain grapefruit seed extract, or extracts made from the seed and other fruit parts such as the peel or pulp. Many commercial products contained large numbers of flavonoids, indicating the use of peel, pulp, or seed coat. This method also permitted detection of synthetic preservatives such as benzethonium chloride, methylparaben, and triclosan in commercial grapefruit seed extract products. Out of the 17 commercial products analyzed, two contained the synthetic antimicrobial agent benzethonium chloride. C1 [Avula, Bharathi; Sagi, Satyanarayanaraju; Wang, Yan-Hong; Wang, Mei; Khan, Ikhlas A.] Univ Mississippi, Pharmaceut Sci Res Inst, Natl Ctr Nat Prod Res, University, MS 38677 USA. [Gafner, Stefan] Amer Bot Council, Austin, TX USA. [Manthey, John A.] ARS, US Hort Res Lab, USDA, Ft Pierce, FL USA. [Khan, Ikhlas A.] Univ Mississippi, Sch Pharm, Dept BioMol Sci, Div Pharmacognosy, University, MS 38677 USA. RP Khan, IA (reprint author), Univ Mississippi, Natl Ctr Nat Prod Res, POB 1848,1558 Univ Circle,MS 38677, University, MS 38677 USA. EM ikhan@olemiss.edu FU "Science Based Authentication of Dietary Supplements" - Food and Drug Administration [1U01FD004246-05]; United States Department of Agriculture, Agricultural Research Service, Specific Cooperative Agreement [58-6408-1-603-07] FX This research is supported in part by "Science Based Authentication of Dietary Supplements" funded by the Food and Drug Administration grant number 1U01FD004246-05, the United States Department of Agriculture, Agricultural Research Service, Specific Cooperative Agreement No. 58-6408-1-603-07. We are grateful to Beatrice Gerhmann and Reto Howald for providing the commercial GFSE samples from Germany and Switzerland, respectively. The authors would like to thank Annette R. Ford for extraction of samples. NR 57 TC 0 Z9 0 U1 17 U2 18 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 EI 1439-0221 J9 PLANTA MED JI Planta Med. PD JUL PY 2016 VL 82 IS 11-12 BP 1058 EP 1069 DI 10.1055/s-0042-107598 PG 12 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA DS0MO UT WOS:000380290900019 PM 27224266 ER PT J AU Crespo, MD Altermann, E Olson, J Miller, WG Chandrashekhar, K Kathariou, S AF Crespo, M. D. Altermann, E. Olson, J. Miller, W. G. Chandrashekhar, K. Kathariou, S. TI Novel plasmid conferring kanamycin and tetracycline resistance in the turkey-derived Campylobacter jejuni strain 11601MD SO PLASMID LA English DT Article DE Campylobacter; C. jejuni; Plasmid; Antibiotic resistance; tet(O); Tetracycline resistance; Kanamycin resistance; aphA-3 ID ANTIBIOTIC-RESISTANCE; COLI; POULTRY; ARTEMIS; TOOL AB In Campylobacter spp., resistance to the antimicrobials kanamycin and tetracycline is frequently associated with plasmid-borne genes. However, relatively few plasmids of Campylobacter jejuni have been fully characterized to date. A novel plasmid (p11601MD; 44,095 nt) harboring tet(O) was identified in C. jejuni strain 11601MD, which was isolated from the jejunum of a turkey produced conventionally in North Carolina. Analysis of the p11601MD sequence revealed the presence of a high-GC content cassette with four genes that included tet(O) and a putative aminoglycoside transferase gene (aphA-3) highly similar to kanamycin resistance determinants. Several genes putatively involved in conjugative transfer were also identified on the plasmid. These findings will contribute to a better understanding of the distribution of potentially self-mobilizing plasmids harboring antibiotic resistance determinants in Campylobacter spp. from turkeys and other sources. (C) 2016 Elsevier Inc. All rights reserved. C1 [Crespo, M. D.; Chandrashekhar, K.; Kathariou, S.] North Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. [Altermann, E.] AgRes Ltd Grasslands Res Ctr, Palmerston North, New Zealand. [Altermann, E.] Massey Univ, Riddet Inst, Palmerston North 4442, New Zealand. [Olson, J.] North Carolina State Univ, Dept Biol Sci, Raleigh, NC 27695 USA. [Miller, W. G.] USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. RP Kathariou, S (reprint author), North Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. EM skathar@ncsu.edu FU USDA-NRI Competitive Grant [2008-35201-04664]; USDA-NIFA grant [2011-2936/2011-51110-31050] FX This research was partially supported by USDA-NRI Competitive Grant 2008-35201-04664 and USDA-NIFA grant 2011-2936/2011-51110-31050. We are grateful to Gregory Wray and the Genome Sequencing and Analysis Core Resource at Duke University for carrying out the sequencing. We thank all members of our laboratory for feedback and support. NR 26 TC 0 Z9 0 U1 5 U2 6 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0147-619X EI 1095-9890 J9 PLASMID JI Plasmid PD JUL PY 2016 VL 86 BP 32 EP 37 DI 10.1016/j.plasmid.2016.06.001 PG 6 WC Genetics & Heredity; Microbiology SC Genetics & Heredity; Microbiology GA DS2FZ UT WOS:000380579100005 PM 27268853 ER PT J AU Hardegree, SP Jones, TA Roundy, BA Shaw, NL Monaco, TA AF Hardegree, Stuart P. Jones, Thomas A. Roundy, Bruce A. Shaw, Nancy L. Monaco, Thomas A. TI Assessment of Range Planting as a Conservation Practice SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE ecosystem services; microclimate; plant materials; seeding; site preparation; weather ID GRASS SEEDLING EMERGENCE; PINYON-JUNIPER WOODLANDS; NORTHERN GREAT-PLAINS; BOTTLEBRUSH SQUIRRELTAIL GERMPLASM; MECHANICAL SEEDBED PREPARATION; TAENIATHERUM-CAPUT-MEDUSAE; NATIVE TALLGRASS PRAIRIE; SOIL-WATER AVAILABILITY; CARBON-DIOXIDE EXCHANGE; NET ECOSYSTEM EXCHANGE AB Natural Resource Conservation Service Range Planting-Conservation Practice Standards provide guidelines for making decisions about seedbed preparation, planting methods, plant materials selection, seeding rate, seeding depth, timing of seeding, postplanting management, and weed control. Adoption of these standards is expected to contribute to successful improvement of vegetation composition and productivity of grazed plant communities. Also expected are some specific conservation effects, such as improved forage for livestock; improved forage, browse, or cover for wildlife; improved water quality and quantity; reduced wind or water erosion; and increased carbon sequestration. The success of specific conservation practices and the magnitude of conservation effects are highly dependent on ecological-site characteristics, the initial degree of deviation from desired site characteristics, and weather, all of which are highly variable in both time and space. Previous research has produced few studies directly linking range planting conservation practices to conservation effects. Assessment of conservation effects attributed to rangeland planting practices must, therefore, be separated into two components: 1) evidence of the degree to which specific management practices have been shown to result in desirable vegetation change and 2) evidence supporting positive conservation effects of alternative vegetation states. The aggregate literature generally supports both 1) the existing conservation practice recommendations for rangeland seeding and 2) the inherent assumption that if these practices are successful, they will result in beneficial conservation effects. High spatial and temporal variability in these systems, however, may limit the success of generic or prescriptive management practices. Current conservation practice recommendations could be improved by incorporating more direct linkages to the ecologically based technical literature, more up-to-date information on adaptive management strategies in highly variable rangeland systems, and integration of monitoring strategies designed to directly test the efficacy of specific conservation practices. Published by Elsevier Inc. on behalf of The Society for Range Management. C1 [Hardegree, Stuart P.] USDA ARS, Northwest Watershed Res Ctr, Boise, ID 83712 USA. [Jones, Thomas A.] USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA. [Roundy, Bruce A.] Brigham Young Univ, Dept Plant & Wildlife Sci, Provo, UT 84602 USA. [Shaw, Nancy L.] USDA, Forest Serv, Rocky Mt Res Stn, Boise, ID 83702 USA. [Monaco, Thomas A.] USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA. RP Hardegree, SP (reprint author), USDA ARS, Northwest Watershed Res Ctr, Boise, ID 83712 USA. EM stuart.hardegree@ars.usda.gov NR 328 TC 1 Z9 1 U1 16 U2 17 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD JUL PY 2016 VL 69 IS 4 BP 237 EP 247 DI 10.1016/j.rama.2016.04.007 PG 11 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DR9TV UT WOS:000380239900001 ER PT J AU Nafus, AM Svejcar, TJ Davies, KW AF Nafus, Aleta M. Svejcar, Tony J. Davies, Kirk W. TI Disturbance History, Management, and Seeding Year Precipitation Influences Vegetation Characteristics of Crested Wheatgrass Stands SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE Agropyron cristatum; burning; grazing; legacy effects; sagebrush; seedbed ID WYOMING BIG SAGEBRUSH; GRASS AGROPYRON-CRISTATUM; GRAZING EXCLUSION; INTRODUCED GRASS; RUSSIAN WILDRYE; PERENNIAL GRASS; NATIVE PRAIRIE; GREAT-PLAINS; SAGE-GROUSE; RECOVERY AB Crested wheatgrass (Agropyron cristatum [L] Gaertm. and Agropyron desertorum [Fisch.] Schult.) has been seeded across millions of hectares of the sagebrush steppe and is often associated with native species displacement and low biological diversity. However, native vegetation composition of these seedings can be variable. To gain better understanding of the correlation between vegetation characteristics of crested wheatgrass seedings and their seeding history and management, we evaluated 121 crested wheatgrass seedings across a 54 230-km(2) area in southeastern Oregon. Higher precipitation in the year following seeding of crested wheatgrass has long-term, negative effects on Wyoming big sagebrush (Artemisia tridentata Nutt. subsp. wyomingensis Beetle & Young) cover and density. Wyoming big sagebrush cover and density were positively correlated with age of seeding and time since fire. We also found that preseeding disturbance (burned, scarified, plowed, or herbicide) appears to have legacy effects on plant community characteristics. For example, herbicide-treated sites had significantly fewer shrubs than sites that were burned or scarified preseeding. Native vegetation cover and density were greater in grazed compared with ungrazed crested wheatgrass stands. The results of this study suggest a number of factors influence native vegetation cover and density within stands of seeded crested wheatgrass. Though disturbance history and precipitation following seeding can't be modified, management actions may affect the cover and abundance of native vegetation in crested wheatgrass stands. Notably, grazing may reduce monoculture characteristics of crested wheatgrass stands and fire exclusion may promote sagebrush and perennial forbs. Published by Elsevier Inc. on behalf of The Society for Range Management. C1 [Nafus, Aleta M.] US Dept Interior, Bur Land Management, Las Vegas, NV 89130 USA. [Svejcar, Tony J.; Davies, Kirk W.] USDA ARS, Burns, OR 97720 USA. RP Davies, KW (reprint author), USDA ARS, Burns, OR 97720 USA. EM kirk.davies@oregonstate.edu FU Great Basin Native Plant Project, Oregon Dept of Fish and Wildlife; ARS FX This research was funded in part by the Great Basin Native Plant Project, Oregon Dept of Fish and Wildlife, and the ARS NR 77 TC 1 Z9 1 U1 4 U2 6 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD JUL PY 2016 VL 69 IS 4 BP 248 EP 256 DI 10.1016/j.rama.2016.03.003 PG 9 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DR9TV UT WOS:000380239900002 ER PT J AU Byars, JA Singh, M AF Byars, Jeffrey A. Singh, Mukti TI Rheological and textural properties of pulse starch gels SO STARCH-STARKE LA English DT Article DE Pasting; Pulses; Rheology; Syneresis; Texture ID LENTIL STARCH; FUNCTIONAL-CHARACTERISTICS; FREEZE-THAW; AMYLOPECTIN; PASTES; GELATINIZATION; AMYLOSE; PEA AB The properties of starch gels from black beans, chickpeas, lentils, and navy beans were investigated. Differences were shown among starch sources, and the effect of starch concentration was studied. Navy bean starch had the highest peak and final viscosities in pasting tests, while black bean starch had the strongest concentration dependence. The viscosity of all starches had similar shear rate dependence at concentrations of 6 and 8%. Navy and black bean starch gels had the highest storage modulus, and black bean starch had the strongest concentration dependence. In creep, navy bean starch had the lowest steady state compliance at 6 and 8%, but there were no significant differences among starches at 10%. Texture analysis showed that navy bean starch gels were the hardest, and black bean starch gels again had the largest concentration dependence. Navy and black bean starch gels had the highest syneresis index after one day, but the syneresis index became similar for all starches at each concentration at longer times. C1 [Byars, Jeffrey A.; Singh, Mukti] ARS, Funct Foods Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. RP Byars, JA (reprint author), ARS, Funct Foods Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM jeffrey.byars@ars.usda.gov NR 22 TC 0 Z9 0 U1 7 U2 9 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA POSTFACH 101161, 69451 WEINHEIM, GERMANY SN 0038-9056 EI 1521-379X J9 STARCH-STARKE JI Starch-Starke PD JUL PY 2016 VL 68 IS 7-8 SI SI BP 778 EP 784 DI 10.1002/star.201500271 PG 7 WC Food Science & Technology SC Food Science & Technology GA DR8OZ UT WOS:000380158500024 ER PT J AU Arslan, ZF Williams, MM Becker, R Fritz, VA Peachey, RE Rabaey, TL AF Arslan, Zubeyde Filiz Williams, Martin M., II Becker, Roger Fritz, Vincent A. Peachey, R. Ed Rabaey, Tom L. TI Alternatives to Atrazine for Weed Management in Processing Sweet Corn SO WEED SCIENCE LA English DT Article DE Herbicide regulation; integrated weed management; North Central Region; Pacific Northwest; sweet corn industry ID HPPD-INHIBITING HERBICIDES; SENSITIVITY; MESOTRIONE AB Atrazine has been the most widely used herbicide in North American processing sweet corn for decades; however, increased restrictions in recent years have reduced or eliminated atrazine use in certain production areas. The objective of this study was to identify the best stakeholder-derived weed management alternatives to atrazine in processing sweet corn. In field trials throughout the major production areas of processing sweet corn, including three states over 4 yr, 12 atrazine-free weed management treatments were compared to three standard atrazine-containing treatments and a weed-free check. Treatments varied with respect to herbicide mode of action, herbicide application timing, and interrow cultivation. All treatments included a PRE application of dimethenamid. No single weed species occurred across all sites; however, weeds observed in two or more sites included common lambsquarters, giant ragweed, morningglory species, velvetleaf, and wild-proso millet. Standard treatments containing both atrazine and mesotrione POST provided the most efficacious weed control among treatments and resulted in crop yields comparable to the weed-free check, thus demonstrating the value of atrazine in sweet corn production systems. Timely interrow cultivation in atrazine-free treatments did not consistently improve weed control. Only two atrazine-free treatments consistently resulted in weed control and crop yield comparable to standard treatments with atrazine POST: treatments with tembotrione POST either with or without interrow cultivation. Additional atrazine-free treatments with topramezone applied POST worked well in Oregon where small-seeded weed species were prevalent. This work demonstrates that certain atrazine-free weed management systems, based on input from the sweet corn growers and processors who would adopt this technology, are comparable in performance to standard atrazine-containing weed management systems. C1 [Arslan, Zubeyde Filiz] Duzce Univ, Duzce, Turkey. [Arslan, Zubeyde Filiz] Univ Illinois, Dept Crop Sci, 1102 S Goodwin Ave, Urbana, IL 61801 USA. [Williams, Martin M., II] USDA ARS, Global Change & Photosynth Res, 1102 S Goodwin Ave, Urbana, IL 61801 USA. [Becker, Roger] Univ Minnesota, Dept Agron & Plant Genet, 411 Borlaug Hall,1991 Upper Buford Circle, St Paul, MN 55108 USA. [Fritz, Vincent A.] Univ Minnesota, Southern Res & Outreach Ctr, Dept Hort Sci, 35838 120th St, Waseca, MN 56093 USA. [Peachey, R. Ed] Oregon State Univ, Dept Hort, 4017 Ag & Life Sci Bldg, Corvallis, OR 97331 USA. [Rabaey, Tom L.] Gen Mills Agr Res, 1201 N 4th St, Le Sueur, MN 56058 USA. RP Williams, MM (reprint author), USDA ARS, Global Change & Photosynth Res, 1102 S Goodwin Ave, Urbana, IL 61801 USA. EM martin.williams@ars.usda.gov FU TUBITAK (Scientific and Technological Council of Turkey); National Institute of Food and Agriculture, U.S. Department of Agriculture [2012-03266] FX The authors greatly appreciate the technical support of Nick Hausman, Tom Hoverstand, Jim Moody, Charlie Rohwer and the crews at the University of Illinois and University of Minnesota. We thank TUBITAK (Scientific and Technological Council of Turkey) for supporting Z. F. Arslan. This material is based upon work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture (number 2012-03266). Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that also might be suitable. NR 29 TC 0 Z9 0 U1 13 U2 13 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 EI 1550-2759 J9 WEED SCI JI Weed Sci. PD JUL-SEP PY 2016 VL 64 IS 3 BP 531 EP 539 DI 10.1614/WS-D-16-00001.1 PG 9 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DS5BM UT WOS:000380795500016 ER PT J AU Pratt, PD Herdocia, K Valentin, V Makinson, J Purcell, MF Mattison, E Rayamajhi, MB Raghu, S Moran, P AF Pratt, P. D. Herdocia, K. Valentin, V. Makinson, J. Purcell, M. F. Mattison, E. Rayamajhi, M. B. Raghu, S. Moran, P. TI Development Rate, Consumption, and Host Specificity of Carea varipes (Lepidoptera: Nolidae) SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE host specificity; weed biological control; assimilation; Rhodomyrtus tomentosa ID RHODOMYRTUS-TOMENTOSA MYRTACEAE; BIOLOGICAL-CONTROL; WEED AB The Asian shrub Rhodomyrtus tomentosa is an invasive weed in Florida and Hawaii, USA. Surveys for natural enemies of this exotic shrub in Hong Kong, China, resulted in the development of a laboratory colony and initial host range testing of Carea varipes Walker as a potential biological control agent of R. tomentosa. Twelve critical test plant species were presented to C. varipes larvae, but complete development was limited to R. tomentosa, the ornamental Myrtus communis, and the Florida native Morella cerifera. Total development time was >10 d faster when held with R. tomentosa and M. communis versus M. cerifera, with the latter species requiring an extra larval instar to reach the pupal stage. Total consumption levels over an individual's larval stage were similar among R. tomentosa and M. cerifera but, when the longer development time associated with the latter host is accounted for, consumption rates indicate that larvae consumed >2 times more leaf material when held with the Florida native. Despite an apparent preference for R. tomentosa, it is clear that the physiological host range of C. varipes includes the Florida native M. cerifera and the ornamentally important M. communis. The generalized feeding patterns exhibited in this research indicates that additional resources dedicated to the development of this species as a biological control agent are unwarranted. C1 [Pratt, P. D.; Moran, P.] USDA ARS, Exot & Invas Weeds Res Unit, 800 Buchanan St, Albany, CA 94710 USA. [Herdocia, K.; Valentin, V.] St Thomas Univ, Dept Biol, 16401 NW 37th Ave, Miami Gardens, FL 33054 USA. [Makinson, J.; Purcell, M. F.; Raghu, S.] USDA ARS, CSIRO Ecosyst Sci, Australian Biol Control Lab, GPO Box 2583, Brisbane, Qld 4001, Australia. [Mattison, E.; Rayamajhi, M. B.] USDA ARS, Invas Plant Res Lab, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. RP Pratt, PD (reprint author), USDA ARS, Exot & Invas Weeds Res Unit, 800 Buchanan St, Albany, CA 94710 USA. EM Paul.Pratt@ars.usda.gov; kherdocia@stu.edu; vvalentin@stu.edu; Jeff.Makinson@csiro.au; matthew.purcell@csiro.au; Min.Rayamajhi@ars.usda.gov; Raghu.Sathyamurthy@csiro.au; patrick.moran@ars.usda.gov FU St. Thomas University; USDA-HSI FX We thank two anonymous reviewers for comments on earlier versions of the manuscript. We thank the support from St. Thomas University and the USDA-HSI-funded grant consortium FCCAgE, its staff, and the USDA agency directors and supervisors. NR 23 TC 0 Z9 0 U1 7 U2 7 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD JUL PY 2016 VL 109 IS 4 BP 513 EP 517 DI 10.1093/aesa/saw021 PG 5 WC Entomology SC Entomology GA DR9MK UT WOS:000380220600002 ER PT J AU Yao, YX Duan, JJ Hopper, KP Mottern, JL Gates, MW AF Yao, Yan-Xia Duan, Jian J. Hopper, Keith P. Mottern, Jason L. Gates, Michael W. TI A New Species of Oobius Trjapitzin (Hymenoptera: Encyrtidae) From the Russian Far East That Parasitizes Eggs of Emerald Ash Borer (Coleoptera: Buprestidae) SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE new species; egg parasitoid; identification; invasive wood borer; biological control ID AGRILI HYMENOPTERA; BIOLOGICAL-CONTROL; PARASITOIDS; CHINA; TOOL; USA AB We describe a new species of egg parasitoid, Oobius primorskyensis Yao & Duan n. sp., collected from the emerald ash borer Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) near Vladivostok, Russia. Morphological and molecular analyses indicate that this taxon is sufficiently distinct from Oobius agrili Zhang & Huang 2005 to merit species status. Furthermore, DNA sequence divergence between O. primorskyensis and specimens in culture of Chinese origin suggest that O. primorskyensis is molecularly distinct from Oobius previously imported into the United Stated and released as biological control agents of emerald ash borer. Laboratory observations showed that O. primorskyensis also differs from O. agrili in diapause pattern. When reared with A. planipennis eggs at 25 +/- 1.5 degrees C, 65 +/- 10% relative humidity, and a photoperiod of 16:8 (L:D) h, nearly 100% of O. primorskyensis diapaused at the second and third generations after their parents had diapaused, whereas a majority (60-96%) of O. agrili did not diapauses for 11 sequential generations. Diagnosis of the species with respect to O. agrili is provided. The new species is a natural enemy of A. planipennis in its native range and may be considered for introduction against A. planipennis in North America. C1 [Yao, Yan-Xia] Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, China State Forestry Adm, Key Lab Forest Protect, Beijing 100091, Peoples R China. [Yao, Yan-Xia; Duan, Jian J.; Hopper, Keith P.] ARS, USDA, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. [Mottern, Jason L.; Gates, Michael W.] ARS, USDA, Systemat Entomol Lab, Washington, DC 20013 USA. RP Duan, JJ (reprint author), ARS, USDA, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. EM yanxia.yao1@gmail.com; jian.duan@ars.usda.gov; keith.hopper@ars.usda.gov; jason.mottern@ars.usda.gov; michael.gates@ars.usda.gov FU USDA Forest Service International Program Grant [60-1926-2-043]; China Scholarship Council (CSC) Fund [201403270017] FX We thank Galina Yurchenko (The Far East Research Institute, Forest management Agency of the Russian Federation) for providing logistic support for the collection of emerald ash borer eggs and associated parasitoids near Vladivostok. We also thank Serguei V. Triapitsyn (Entomology Research Museum, Department of Entomology, University of California, Riverside, CA) for providing literature and constructive comments of the manuscript. John S. Noyes (Natural History Museum, London, United Kingdom) provided us the book chapter on Oobius, which was published in his book entitled "Encyrtidae of Costa Rica" (2010). Kristi Larson and Jonathan Schmude at USDA ARS BIIRU reared the parasitoid from eggs of A. planipennis, provided materials, and helped with photographs and laboratory observations. We also appreciate comments by Zhongqi Yang (Chinese Academy of Forestry, Beijing, China). The project was funded in part by USDA Forest Service International Program Grant (60-1926-2-043) and China Scholarship Council (CSC) Fund (201403270017). USDA is an equal opportunity provider and employer. NR 40 TC 0 Z9 0 U1 14 U2 18 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD JUL PY 2016 VL 109 IS 4 BP 629 EP 638 DI 10.1093/aesa/saw022 PG 10 WC Entomology SC Entomology GA DR9MK UT WOS:000380220600012 ER PT J AU Hamerlynck, EP Sheley, RL Davies, KW Svejcar, TJ AF Hamerlynck, Erik P. Sheley, Roger L. Davies, Kirk W. Svejcar, Tony J. TI Postdefoliation ecosystem carbon and water flux and canopy growth dynamics in sagebrush steppe bunchgrasses SO ECOSPHERE LA English DT Article DE bluebunch wheatgrass; ecosystem respiration; evapotranspiration; grazing; gross ecosystem photosynthesis; leaf area index; net ecosystem carbon dioxide exchange; specific leaf mass; squirreltail; water use efficiency ID NORTHERN TEMPERATE GRASSLAND; SEMIARID GRASSLAND; DIOXIDE FLUXES; GAS-EXCHANGE; CO2 EXCHANGE; INTERANNUAL VARIABILITY; BIOMASS PRODUCTION; PACIFIC-NORTHWEST; CHIHUAHUAN DESERT; RAINFALL REGIMES AB Developing land-use practices that lead to sustainable net primary productivity in rangelands are important, but understanding their consequences to population and community processes is not often accounted for in basic ecosystem studies. Grazed and ungrazed upland ecosystems generally do not differ in net ecosystem CO2 exchange (NEE), but the underlying mechanisms and the concurrent effects of defoliation to vegetative and reproductive biomass allocation are unclear. To address this, we measured evapotranspiration (ET), NEE, and its constituent fluxes of ecosystem respiration (R-eco) and gross ecosystem photosynthesis (GEP) with live canopy leaf area index (LAI(live); m(2) live leaf area/m(2) ground area) and aboveground leaf, culm, and reproductive biomass in plots of clipped and unclipped squirreltail (Elymus elymoides) and bluebunch wheatgrass (Pseudoroegneria spicata) growing in intact sagebrush steppe. Clipping reduced LAI(live) by 75%, but subsequent re-growth rates in clipped plots was similar to LAI(live) accumulation in unclipped plots. Concurrently, ET and NEE was similar between clipped and unclipped plots, with NEE primarily determined by GEP. GEP was initially lower in clipped plots, but then converged with unclipped GEP even as LAI(live) continued to increase in both treatments. GEP convergence was driven by higher whole-plant photosynthesis (GEP(live) = GEP/LAI(live)) in clipped plots. Ecosystem water use efficiency (GEP/ET) was reduced by 16% with clipping, due to low GEP/ET 2 weeks following defoliation, but GEP/ET converged before GEP levels did. Proportional reproductive biomass was higher in E. elymoides (21.4% total biomass) than in P. spicata (0.5% total biomass) due to lower allocation to specific leaf and culm mass. Clipping reduced reproductive effort in E. elymoides, in terms of total reproductive biomass (-56%), seed mass per unit leaf area (-64%), and seed mass per flowering head (-77%). We concluded defoliation increased canopy-level light penetration, facilitating rapid recovery of ecosystem fluxes, but that allocation to vegetative regrowth supporting this led to lower reproductive effort in these range grasses. Insights from studies such as this will be useful in formulating systems-based land management strategies aimed at maintaining annual productivity and long-term population and community goals in semiarid range-land ecosystems. C1 [Hamerlynck, Erik P.; Sheley, Roger L.; Davies, Kirk W.; Svejcar, Tony J.] USDA ARS, Eastern Oregon Agr Res Ctr, 67826-A Highway 205, Burns, OR 97720 USA. RP Hamerlynck, EP (reprint author), USDA ARS, Eastern Oregon Agr Res Ctr, 67826-A Highway 205, Burns, OR 97720 USA. EM erik.hamerlynck@oregonstate.edu NR 73 TC 0 Z9 0 U1 12 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD JUL PY 2016 VL 7 IS 7 AR e01376 DI 10.1002/ecs2.1376 PG 21 WC Ecology SC Environmental Sciences & Ecology GA DR6OF UT WOS:000380020500002 ER PT J AU Landry, EJ Fuchs, SJ Hu, JG AF Landry, Erik J. Fuchs, Sam J. Hu, Jinguo TI Carbohydrate composition of mature and immature faba bean seeds SO JOURNAL OF FOOD COMPOSITION AND ANALYSIS LA English DT Article DE Vicia faba; Faba bean (fava bean); Raffinose-family oligosaccharides; Food analysis; Food composition; Nutritional quality; High performance liquid chromatography ID COAT-ASSOCIATED INVERTASES; VICIA-FABA; ALPHA-GALACTOSIDES; L CULTIVARS; OLIGOSACCHARIDES; STARCH; EXPRESSION; SUGARS; PEA AB Faba bean is a valuable pulse crop for human consumption. The low molecular weight carbohydrates (LMWC): glucose, fructose, sucrose (GFS), raffinose, stachyose, and verbascose (RFO- raffinose family oligosaccharides) in faba bean seeds contribute to the flavor and prebiotic nature of this edible bean. Understanding the variation of these compounds across the species would aid plant breeders in their selection efforts to release improved varieties. Therefore, this study was conducted to quantify LMWCs from a diverse collection of faba bean germplasm. The LMWCs of mature and immature seed from 40 faba bean populations across a range of seed sizes (26.2-172.0 g 100 seed(-1)) were quantified with an Agilent 1260 Infinity LC (size exclusion chromatography/gel permeation chromatography) system with refractive index detection. Sucrose was the predominant constituent LMWC of immature seeds ranging from 5.9 to 22.6% DW for cotyledons and 6.7 to 16.7% DW for seed coats, while total RFO averaged <1% DW across populations. The sucrose content of mature seeds was relatively stable across population with a mean of 2.4% DW, while RFO content ranged from 2.5 to 7.5% DW. The apparent positive relationship between seed size and GFS of immature seed and sucrose and RFO of mature seed indicates that selection for seed size may also affect LMWC concentration. Published by Elsevier Inc. C1 [Landry, Erik J.; Fuchs, Sam J.; Hu, Jinguo] Washington State Univ, Western Reg Plant Intro Stn, ARS, USDA, Pullman, WA 99164 USA. RP Landry, EJ (reprint author), Washington State Univ, Western Reg Plant Intro Stn, ARS, USDA, Pullman, WA 99164 USA. EM erik.landry@ars.usda.gov FU ARCS(R) Foundation; USDA ARS CRIS Project [5348-21000-026-00D]; USDA NIFA Multistate Research Project [W-006] FX The authors would like to thank the ARCS (R) Foundation for a scholarship provided to Erik Landry and funding from USDA ARS CRIS Project 5348-21000-026-00D and USDA NIFA Multistate Research Project W-006. NR 40 TC 0 Z9 0 U1 9 U2 11 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0889-1575 EI 1096-0481 J9 J FOOD COMPOS ANAL JI J. Food Compos. Anal. PD JUL PY 2016 VL 50 BP 55 EP 60 DI 10.1016/j.jfca.2016.05.010 PG 6 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DS2NU UT WOS:000380621600008 ER PT J AU Choi, SH Kozukue, N Kim, HJ Friedman, M AF Choi, Suk-Hyun Kozukue, Nobuyuki Kim, Hyun-Jeong Friedman, Mendel TI Analysis of protein amino acids, non-protein amino acids and metabolites, dietary protein, glucose, fructose, sucrose, phenolic, and flavonoid content and antioxidative properties of potato tubers, peels, and cortexes (pulps) SO JOURNAL OF FOOD COMPOSITION AND ANALYSIS LA English DT Article DE Food analysis; Food composition; Whole potatoes; Potato peels; Potato pulp; Water content; Amino acids; Amino acid metabolites; Protein; Phenolics; Flavonoids; Antioxidative effects; Research needs ID BETA-AMINOISOBUTYRIC ACID; GAMMA-AMINOBUTYRIC-ACID; NUTRITIONAL-VALUE; CANCER-CELLS; ACRYLAMIDE FORMATION; JUJUBE FRUIT; ASPARAGINE; INHIBITION; GLYCOALKALOIDS; VARIETIES AB The composition and antioxidative activity of whole potato tubers from five Korean cultivars, three peels from one cultivar, and eight pulps (cortexes) after peeling from six different cultivars were evaluated. Whole tubers were sectioned into three parts followed by analysis of the peels and pulps of each part. The following characteristics were determined: the dimensions and water content of whole tubers; nutritional protein content consisting of protein and free amino acids by an automated Kjeldahl nitrogen assay; free non-protein amino acids, metabolites, and fructose, glucose, and sucrose content by HPLC; phenolic compounds by HPLC and LC/MS; total phenolics and flavonoids by colorimetry; and antioxidative properties by ABTS, DPPH, and FRAP assays. The results demonstrate differences and similarities in the content of nutritional and bioactive compounds and in their bioactivities from whole potatoes, peels, and pulps. The present study reports for the first time the analysis of multiple potato nutrients and bioactive components as well the antioxidative properties of whole potatoes, peels, and pulps derived from the same tubers. The described methods are expected to facilitate the analysis of commercial and newly-developed potato cultivars, peels, and pulps and relate their composition and antioxidative activities to their reported nutritional, health-promoting, and industrial properties. Published by Elsevier Inc. C1 [Choi, Suk-Hyun; Kozukue, Nobuyuki] Seowon Univ, Dept Food Serv & Culinary Arts, Cheongju 362742, South Korea. [Kim, Hyun-Jeong] Keimyung Univ, Ctr Tradit Microorganism Resources, Daegu 704701, South Korea. [Friedman, Mendel] ARS, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. RP Friedman, M (reprint author), ARS, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM mosimosi@seowon.ac.kr; Kozukue777@ybb.ne.jp; sarikhj@kmu.ac.kr; Mendel.Friedman@ars.usda.gov OI Friedman, Mendel/0000-0003-2582-7517 NR 58 TC 2 Z9 2 U1 26 U2 35 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0889-1575 EI 1096-0481 J9 J FOOD COMPOS ANAL JI J. Food Compos. Anal. PD JUL PY 2016 VL 50 BP 77 EP 87 DI 10.1016/j.jfca.2016.05.011 PG 11 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DS2NU UT WOS:000380621600011 ER PT J AU Huang, XY Deng, FL Yamaji, N Pinson, SRM Fujii-Kashino, M Danku, J Douglas, A Guerinot, ML Salt, DE Ma, JF AF Huang, Xin-Yuan Deng, Fenglin Yamaji, Naoki Pinson, Shannon R. M. Fujii-Kashino, Miho Danku, John Douglas, Alex Guerinot, Mary Lou Salt, David E. Ma, Jian Feng TI A heavy metal P-type ATPase OsHMA4 prevents copper accumulation in rice grain SO NATURE COMMUNICATIONS LA English DT Article ID QUANTITATIVE TRAIT LOCI; AGROBACTERIUM-MEDIATED TRANSFORMATION; HETEROGENEOUS INBRED FAMILY; SPATIAL GENETIC-STRUCTURE; ARABIDOPSIS-THALIANA; LOCALIZED TRANSPORTER; CULTIVATED RICE; SALT TOLERANCE; CD TOLERANCE; SWISS-MODEL AB Rice is a major source of calories and mineral nutrients for over half the world's human population. However, little is known in rice about the genetic basis of variation in accumulation of copper (Cu), an essential but potentially toxic nutrient. Here we identify OsHMA4 as the likely causal gene of a quantitative trait locus controlling Cu accumulation in rice grain. We provide evidence that OsHMA4 functions to sequester Cu into root vacuoles, limiting Cu accumulation in the grain. The difference in grain Cu accumulation is most likely attributed to a single amino acid substitution that leads to different OsHMA4 transport activity. The allele associated with low grain Cu was found in 67 of the 1,367 rice accessions investigated. Identification of natural allelic variation in OsHMA4 may facilitate the development of rice varieties with grain Cu concentrations tuned to both the concentration of Cu in the soil and dietary needs. C1 [Huang, Xin-Yuan; Danku, John; Douglas, Alex; Salt, David E.] Univ Aberdeen, Inst Biol & Environm Sci, Aberdeen AB24 3UU, Scotland. [Deng, Fenglin; Yamaji, Naoki; Fujii-Kashino, Miho; Ma, Jian Feng] Okayama Univ, Inst Plant Sci & Resources, Kurashiki, Okayama 7100046, Japan. [Pinson, Shannon R. M.] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. [Guerinot, Mary Lou] Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA. RP Salt, DE (reprint author), Univ Aberdeen, Inst Biol & Environm Sci, Aberdeen AB24 3UU, Scotland.; Ma, JF (reprint author), Okayama Univ, Inst Plant Sci & Resources, Kurashiki, Okayama 7100046, Japan. EM david.salt@abdn.ac.uk; maj@rib.okayama-u.ac.jp RI Danku, John/C-3477-2014 OI Danku, John/0000-0002-5103-3852 FU JSPS KAKENHI [16H06296]; US National Science Foundation, Plant Genome Research Program [IOS 0701119] FX We thank B. Lahner, E. Yakubova and S. Rikiishi for ICP-MS analysis, N. Komiyama, Iowa State University Plant Transformation Facility and Prashant Hosmani for generation of transgenic rice, K. Wang for providing pTF101.1 vector and N. Verbruggen for providing pYES2 and pYEC2/CT-GFP vectors. We also thank Rice T-DNA Insertion Sequence Database center for providing the T-DNA insertion line and X. Wang, T. Zheng and Z. Li for accessing 3K rice genome sequence, and Graeme Paton for helpful discussions on Cu bioavailability in water-logged soils. This research was supported by a Grant-in-Aid for Specially promoted Research (JSPS KAKENHI Grant Number 16H06296 to J.F.M), and the US National Science Foundation, Plant Genome Research Program (Grant #IOS 0701119 to D.E.S., M.L.G. and S.R.M.P.). NR 64 TC 0 Z9 0 U1 11 U2 15 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD JUL PY 2016 VL 7 AR 12138 DI 10.1038/ncomms12138 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DS0RS UT WOS:000380305000001 PM 27387148 ER PT J AU Evans, JA Lankau, RA Davis, AS Raghu, S Landis, DA AF Evans, Jeffrey A. Lankau, Richard A. Davis, Adam S. Raghu, S. Landis, Douglas A. TI Soil-mediated eco-evolutionary feedbacks in the invasive plant Alliaria petiolata SO FUNCTIONAL ECOLOGY LA English DT Article DE Alliaria petiolata; eco-evolutionary; eco-evolutionary feedbacks; ecology; evolution; reciprocal feedbacks; sinigrin; soil root feedback ID RAPID EVOLUTION; ECOLOGICAL DYNAMICS; POPULATION-DYNAMICS; MANAGEMENT; COMMUNITIES; IMPACT; TIME; CONSERVATION; BRASSICACEAE; BIOLOGY AB Ecological and evolutionary processes historically have been assumed to operate on significantly different time-scales. We know now from theory and work in experimental and model systems that these processes can feed back on each other on mutually relevant time-scales. Here, we present evidence of a soil-mediated eco-evolutionary feedback on the population dynamics of an invasive biennial plant, Alliaria petiolata. As populations age, natural selection drives down production of A.petiolata's important antimycorrhizal allelochemical, sinigrin. This occurs due to density-dependent selection on sinigrin, which is favoured under interspecific, but disfavoured under intraspecific, competition. We show that population stochastic growth rates ((S)) and plant densities are positively related to sinigrin concentration measured in seedling roots. This interaction is mediated by sinigrin's positive effect on seedling and summer survival, which are important drivers of (S). Together, these illustrate how the evolution of a trait shaped by natural selection can influence the ecology of a species over a period of just years to decades, altering its trajectory of population growth and interactions with the species in the soil and plant communities it invades. Our findings confirm the predictions that eco-evolutionary feedbacks occur in natural populations. Furthermore, they improve our conceptual framework for projecting future population growth by linking the variation in plant demography to a critical competitive trait (sinigrin) whose selective advantages decrease as populations age. C1 [Evans, Jeffrey A.; Davis, Adam S.] Univ Illinois, ARS, USDA, Global Change & Photosynth Res Unit, Turner Hall,1102 S Goodwin Ave, Urbana, IL 61801 USA. [Lankau, Richard A.] Univ Georgia, Dept Plant Biol, 2502 Miller Plant Sci, Athens, GA 30602 USA. [Raghu, S.] CSIRO, GPO Box 2583, Brisbane, Qld 4001, Australia. [Raghu, S.] USDA ARS Australian Biol Control Lab, GPO Box 2583, Brisbane, Qld 4001, Australia. [Landis, Douglas A.] Ctr Integrated Plant Syst Lab, 578 Wilson Rd,Room 204, E Lansing, MI 48824 USA. [Lankau, Richard A.] Univ Wisconsin, Dept Plant Pathol, Russell Labs Bldg,1630 Linden Dr, Madison, WI 53706 USA. RP Evans, JA (reprint author), Univ Illinois, ARS, USDA, Global Change & Photosynth Res Unit, Turner Hall,1102 S Goodwin Ave, Urbana, IL 61801 USA. EM jeffrey.evans@ars.usda.gov FU NSF DEB Award [0918450]; USDA CSREES Award [2005-35320-15312]; EPA STAR [FP-91650101]; Michigan State Plant Sciences Fellowship; Howard Hughes Medical Institute Postdoctoral Fellowship in the Life Sciences FX We wish to thank the many field and laboratory assistants who collected data for this project, land owners and managers who provided access to field sites, Rachel Nodurft for assistance with the quantitative genetics study and Doug Schemske for his role in the original demography study. This work was partially supported by NSF DEB Award 0918450, USDA CSREES Award 2005-35320-15312 and fellowships to J. Evans: EPA STAR FP-91650101, The Michigan State Plant Sciences Fellowship and a Howard Hughes Medical Institute Postdoctoral Fellowship in the Life Sciences award to Dartmouth College (R. Sloboda, PI). The authors declare no conflicts of interest. NR 44 TC 3 Z9 3 U1 44 U2 44 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0269-8463 EI 1365-2435 J9 FUNCT ECOL JI Funct. Ecol. PD JUL PY 2016 VL 30 IS 7 BP 1053 EP 1061 DI 10.1111/1365-2435.12685 PG 9 WC Ecology SC Environmental Sciences & Ecology GA DR5YK UT WOS:000379978100005 ER PT J AU Lippitt, CD Stow, DA Riggan, PJ AF Lippitt, Christopher D. Stow, Douglas A. Riggan, Philip J. TI Application of the remote-sensing communication model to a time-sensitive wildfire remote-sensing system SO INTERNATIONAL JOURNAL OF REMOTE SENSING LA English DT Article ID NATURAL HAZARDS; FIRE DETECTION; SATELLITE; INFORMATION; MANAGEMENT; DISASTERS AB Remote sensing for hazard response requires a priori identification of sensor, transmission, processing, and distribution methods to permit the extraction of relevant information in timescales sufficient to allow managers to make a given time-sensitive decision. This study applies and demonstrates the utility of the Remote Sensing Communication Model (RSCM) to improve a tactical wildfire remote-sensing system to better meet the time-sensitive information requirements of emergency response managers in San Diego County, USA. A thermal infrared airborne remote-sensing system designed and operated by the United States Forest Service Pacific Southwest Research Station for active wildfire monitoring is documented and updated based on the RSCM. Analysis of the thermal infrared remote-sensing system in the context of the RSCM identified three configuration changes that can improve the effectiveness of the information produced when employed by wildfire incident commanders for suppression prioritization: (1) limit spectral sampling collection to a single waveband; (2) complete image processing steps on-board the aircraft; and (3) provide information on wildfire locations to incident commanders in the form of a static map. C1 [Lippitt, Christopher D.] Univ New Mexico, Dept Geog & Environm Studies, 1 Univ New Mexico, Albuquerque, NM 87131 USA. [Lippitt, Christopher D.; Stow, Douglas A.] San Diego State Univ, Dept Geog, San Diego, CA 92182 USA. [Riggan, Philip J.] US Forest Serv, USDA, Pacific Southwest Res Stn, Riverside, CA USA. RP Lippitt, CD (reprint author), Univ New Mexico, Dept Geog & Environm Studies, 1 Univ New Mexico, Albuquerque, NM 87131 USA. EM clippitt@unm.edu FU American Recovery and Reinvestment Award (ARRA) through the US Forest Service [JV-11279702-101]; SDSU [G00008499]; Robert N. Colwell memorial Fellowship; San Diego State University Department of Geography FX This work was partially supported by an American Recovery and Reinvestment Award (ARRA) through the US Forest Service (JV-11279702-101) - SDSU [Grant G00008499] and the Robert N. Colwell memorial Fellowship and by the San Diego State University Department of Geography NR 32 TC 2 Z9 2 U1 7 U2 22 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND SN 0143-1161 EI 1366-5901 J9 INT J REMOTE SENS JI Int. J. Remote Sens. PD JUL PY 2016 VL 37 IS 14 BP 3272 EP 3292 DI 10.1080/01431161.2016.1196840 PG 21 WC Remote Sensing; Imaging Science & Photographic Technology SC Remote Sensing; Imaging Science & Photographic Technology GA DR5ON UT WOS:000379952400006 ER PT J AU Su, YJ Guo, QH Collins, BM Fry, DL Hu, TY Kelly, M AF Su, Yanjun Guo, Qinghua Collins, Brandon M. Fry, Danny L. Hu, Tianyu Kelly, Maggi TI Forest fuel treatment detection using multi-temporal airborne lidar data and high-resolution aerial imagery: a case study in the Sierra Nevada Mountains, California SO INTERNATIONAL JOURNAL OF REMOTE SENSING LA English DT Article ID UNSUPERVISED CHANGE DETECTION; PEARL RIVER DELTA; LEAF-AREA INDEX; LANDSAT-TM DATA; CANOPY COVER; FIRE SEVERITY; NDVI DATA; BIOMASS; LANDSCAPE; ACCURACY AB Treatments to reduce forest fuels are often performed in forests to enhance forest health, regulate stand density, and reduce the risk of wildfires. Although commonly employed, there are concerns that these forest fuel treatments (FTs) may have negative impacts on certain wildlife species. Often FTs are planned across large landscapes, but the actual treatment extents can differ from the planned extents due to operational constraints and protection of resources (e.g. perennial streams, cultural resources, wildlife habitats). Identifying the actual extent of the treated areas is of primary importance to understand the environmental influence of FTs. Light detection and ranging (lidar) is a powerful remote-sensing tool that can provide accurate measurements of forest structures and has great potential for monitoring forest changes. This study used the canopy height model (CHM) and canopy cover (CC) products derived from multi-temporal airborne laser scanning (ALS) data to monitor forest changes following the implementation of landscape-scale FT projects. Our approach involved the combination of a pixel-wise thresholding method and an object-of-interest (OBI) segmentation method. We also investigated forest change using normalized difference vegetation index (NDVI) and standardized principal component analysis from multi-temporal high-resolution aerial imagery. The same FT detection routine was then applied to compare the capability of ALS data and aerial imagery for FT detection. Our results demonstrate that the FT detection using ALS-derived CC products produced both the highest total accuracy (93.5%) and kappa coefficient () (0.70), and was more robust in identifying areas with light FTs. The accuracy using ALS-derived CHM products (the total accuracy was 91.6%, and the was 0.59) was significantly lower than that using ALS-derived CC, but was still higher than using aerial imagery. Moreover, we also developed and tested a method to recognize the intensity of FTs directly from pre- and post-treatment ALS point clouds. C1 [Su, Yanjun; Guo, Qinghua] Univ Calif Merced, Sch Engn, Sierra Nevada Res Inst, Merced, CA USA. [Collins, Brandon M.] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA USA. [Collins, Brandon M.] Univ Calif Berkeley, Ctr Fire Res & Outreach, Berkeley, CA USA. [Fry, Danny L.; Kelly, Maggi] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA USA. [Hu, Tianyu] Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing, Peoples R China. RP Guo, QH (reprint author), Univ Calif Merced, Sch Engn, 5200 North Lake Rd, Merced, CA 95343 USA. EM qguo@ucmerced.edu FU USDA Forest Service Region 5; USDA Forest Service Pacific Southwest Research Station; US Fish and Wildlife Service; California Department of Water Resources; California Department of Fish and Game; California Department of Forestry and Fire Protection; Sierra Nevada Conservancy; National Science Foundation [DBI 1356077]; National Science Foundation of China [41471363, 31270563] FX This is SNAMP Publication Number 47. The Sierra Nevada Adaptive Management Project is funded by USDA Forest Service Region 5, USDA Forest Service Pacific Southwest Research Station, US Fish and Wildlife Service, California Department of Water Resources, California Department of Fish and Game, California Department of Forestry and Fire Protection, and the Sierra Nevada Conservancy. We thank USFS, Tahoe National Forest, American River District personnel for providing the FT boundaries. "Besides, this work is supported by the National Science Foundation [DBI 1356077] and National Science Foundation of China [project numbers 41471363 and 31270563]." We also thank Dr John J. Battles for helpful suggestions on the article. NR 70 TC 1 Z9 1 U1 16 U2 29 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND SN 0143-1161 EI 1366-5901 J9 INT J REMOTE SENS JI Int. J. Remote Sens. PD JUL PY 2016 VL 37 IS 14 BP 3322 EP 3345 DI 10.1080/01431161.2016.1196842 PG 24 WC Remote Sensing; Imaging Science & Photographic Technology SC Remote Sensing; Imaging Science & Photographic Technology GA DR5ON UT WOS:000379952400008 ER PT J AU Madsen, JD Sartain, B Turnage, G Marko, M AF Madsen, John D. Sartain, Bradley Turnage, Gray Marko, Michelle TI Management of flowering rush in the Detroit Lakes, Minnesota SO JOURNAL OF AQUATIC PLANT MANAGEMENT LA English DT Article DE Butomus umbellatus L.; chemical control; diquat; efficacy; emergent plant; invasive plant; native plant diversity; selectivity ID SEASON HERBICIDE TREATMENTS; BUTOMUS-UMBELLATUS; AQUATIC PLANT; EURASIAN WATERMILFOIL; SEED PRODUCTION; WATER-LEVEL; EFFICACY; GROWTH; WATERCHESTNUT; POPULATIONS AB Flowering rush (Butomus umbellatus) is an invasive aquatic plant introduced to North America from Eurasia in 1897. Flowering rush can grow either submersed or emergent from wet soil habitats to waters that are up to 5 m deep. Flowering rush was first observed in the Detroit Lake system in the 1960s, causing significant impact to shoreline and recreational use. Flowering rush is currently found in five basins of the Detroit Lake system: Big Detroit, Little Detroit, Curfman, Sallie, and Melissa Lakes. Submersed treatments with diquat were used during 2012 on an operational scale to control the nuisance impacts of flowering rush in waters from 0 to 1.3 m deep. We evaluated the response of native plant communities with the use of a point intercept method on 30 or more predetermined points in each of nine treatment plots, with four untreated reference plots. Treatment plots were sampled before treatment (June), and 4 wk after each of the two treatments. We also sampled 20 biomass cores (0.018 m(-2)) in each of four treatment and four untreated reference plots. Although some species declined after treatment, most native species did not change significantly after treatments compared to untreated reference plots. Treatments with diquat not only significantly reduce flowering rush distribution (60%) and aboveground biomass (99%), but also significantly reduced belowground biomass (82%) and rhizome bud density (83%). As flowering rush is an herbaceous perennial that propagates predominantly by rhizome buds, reductions in rhizome bud density indicate that this approach can be used for long-term reduction in flowering rush populations. C1 [Madsen, John D.] Univ Calif Davis, Dept Plant Sci, Exot & Invas Weed Res Unit, USDA,ARS, Mail Stop 4,1 Shields Ave, Davis, CA 95616 USA. [Sartain, Bradley] Mississippi State Univ, Plant & Soil Sci Dept, Mississippi State, MS 39762 USA. [Turnage, Gray] Geosyst Res Inst, 1 Res Blvd, Mississippi State, MS 39762 USA. [Marko, Michelle] Concordia Coll, Dept Biol, 901 8th St South, Moorhead, MN 56562 USA. RP Madsen, JD (reprint author), Univ Calif Davis, Dept Plant Sci, Exot & Invas Weed Res Unit, USDA,ARS, Mail Stop 4,1 Shields Ave, Davis, CA 95616 USA. EM jmadsen@ucdavis.edu OI Turnage, Gray/0000-0001-6337-6329 FU Pelican River Watershed District; Minnesota Department of Natural Resources FX This research was supported by the Pelican River Watershed District, with additional support from the Minnesota Department of Natural Resources. Professional Lake Management (PLM) performed the herbicide treatments, and provided information on those treatments. Field and laboratory assistance was provided by students from Mississippi State University and Concordia College, Moor-head, MN. Reviews before submission were provided by Guy Kyser, Chip Welling, and Dr. Ryan Wersal. Mention of a manufacturer does not constitute a warranty or guarantee of the product by the U.S. Department of Agriculture nor an endorsement over other products not mentioned. NR 28 TC 0 Z9 0 U1 5 U2 5 PU AQUATIC PLANT MANAGEMENT SOC, INC PI VICKSBURG PA PO BOX 821265, VICKSBURG, MS 39182 USA SN 0146-6623 J9 J AQUAT PLANT MANAGE JI J. Aquat. Plant Manage. PD JUL PY 2016 VL 54 BP 61 EP 67 PG 7 WC Plant Sciences; Marine & Freshwater Biology SC Plant Sciences; Marine & Freshwater Biology GA DR7JR UT WOS:000380076400002 ER PT J AU Grodowitz, MJ Harms, NE Freedman, JE AF Grodowitz, Michael J. Harms, Nathan E. Freedman, Jan E. TI Use of an inexpensive chlorophyll meter to predict nitrogen levels in leaf tissues of water hyacinth (Eichhornia crassipes) SO JOURNAL OF AQUATIC PLANT MANAGEMENT LA English DT Article ID BIOLOGICAL-CONTROL; SALVINIA-MOLESTA; PLANT; HERBIVORY; GROWTH C1 [Grodowitz, Michael J.] ARS, USDA, Stoneville, MS USA. [Harms, Nathan E.; Freedman, Jan E.] US Army, Engineer Res & Dev Ctr, Vicksburg, MS 39180 USA. RP Grodowitz, MJ (reprint author), ARS, USDA, Stoneville, MS USA. EM michael.grodowitz@ars.usda.gov FU U.S. Army Aquatic Plant Control Research Program FX This research was supported by the U.S. Army Aquatic Plant Control Research Program under the direction of Linda Nelson. Permission was granted by the Chief of Engineers to publish this information. We also thank Aaron Schad, Nathan Beane, and Timothy Lewis for their critical review of the manuscript and Ms. Dian Smith for help in determining leaf nitrogen levels. NR 19 TC 0 Z9 0 U1 2 U2 2 PU AQUATIC PLANT MANAGEMENT SOC, INC PI VICKSBURG PA PO BOX 821265, VICKSBURG, MS 39182 USA SN 0146-6623 J9 J AQUAT PLANT MANAGE JI J. Aquat. Plant Manage. PD JUL PY 2016 VL 54 BP 106 EP 108 PG 3 WC Plant Sciences; Marine & Freshwater Biology SC Plant Sciences; Marine & Freshwater Biology GA DR7JR UT WOS:000380076400009 ER PT J AU Beissinger, TM Wang, L Crosby, K Durvasula, A Hufford, MB Ross-Ibarra, J AF Beissinger, Timothy M. Wang, Li Crosby, Kate Durvasula, Arun Hufford, Matthew B. Ross-Ibarra, Jeffrey TI Recent demography drives changes in linked selection across the maize genome SO NATURE PLANTS LA English DT Article ID HUMAN-POPULATION SIZE; DELETERIOUS MUTATIONS; GENETIC ARCHITECTURE; TRANSPOSABLE ELEMENT; ARABIDOPSIS-THALIANA; ARTIFICIAL SELECTION; POSITIVE SELECTION; LOCAL ADAPTATION; SEQUENCING DATA; HUMAN-EVOLUTION AB Genetic diversity is shaped by the interaction of drift and selection, but the details of this interaction are not well understood. The impact of genetic drift in a population is largely determined by its demographic history, typically summarized by its long-term effective population size (N-e). Rapidly changing population demographics complicate this relationship, however. To better understand how changing demography impacts selection, we used whole-genome sequencing data to investigate patterns of linked selection in domesticated and wild maize (teosinte). We produce the first whole-genome estimate of the demography of maize domestication, showing that maize was reduced to approximately 5% the population size of teosinte before it experienced rapid expansion post-domestication to population sizes much larger than its ancestor. Evaluation of patterns of nucleotide diversity in and near genes shows little evidence of selection on beneficial amino acid substitutions, and that the domestication bottleneck led to a decline in the efficiency of purifying selection in maize. Young alleles, however, show evidence of much stronger purifying selection in maize, reflecting the much larger effective size of present day populations. Our results demonstrate that recent demographic change-a hall-mark of many species including both humans and crops-can have immediate and wide-ranging impacts on diversity that conflict with expectations based on long-term N-e alone. C1 [Beissinger, Timothy M.; Crosby, Kate; Durvasula, Arun; Ross-Ibarra, Jeffrey] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Beissinger, Timothy M.] ARS, USDA, Columbia, MO 65211 USA. [Beissinger, Timothy M.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Wang, Li; Hufford, Matthew B.] Iowa State Univ, Dept Ecol Evolut & Organismal Biol, Ames, IA 50011 USA. [Ross-Ibarra, Jeffrey] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA. [Ross-Ibarra, Jeffrey] Univ Calif Davis, Ctr Populat Biol, Davis, CA 95616 USA. RP Beissinger, TM; Ross-Ibarra, J (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA.; Beissinger, TM (reprint author), ARS, USDA, Columbia, MO 65211 USA.; Beissinger, TM (reprint author), Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA.; Ross-Ibarra, J (reprint author), Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA.; Ross-Ibarra, J (reprint author), Univ Calif Davis, Ctr Populat Biol, Davis, CA 95616 USA. EM beissingert@missouri.edu; rossibarra@ucdavis.edu FU National Science Foundation Plant Genome Research Project [1238014]; US Department of Agriculture (USDA) Agricultural Research Service; USDA Hatch project [CA-D-PLS-2066-H] FX We are indebted to G. Coop and S. Aeschbacher for their constructive input during this study. We thank R. Bukowski and Q. Sun for providing early-access data from maize HapMap3. Funding was provided by National Science Foundation Plant Genome Research Project 1238014, the US Department of Agriculture (USDA) Agricultural Research Service, and USDA Hatch project CA-D-PLS-2066-H. NR 81 TC 4 Z9 4 U1 19 U2 42 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2055-026X EI 2055-0278 J9 NAT PLANTS JI Nat. Plants PD JUL PY 2016 VL 2 IS 7 AR 16084 DI 10.1038/NPLANTS.2016.84 PG 7 WC Plant Sciences SC Plant Sciences GA DS1HM UT WOS:000380346500014 PM 27294617 ER PT J AU Coblentz, W Jokela, W Cavadini, JS AF Coblentz, Wayne Jokela, William Cavadini, Jason S. TI Production and Nitrogen Use Efficiency of Oat Forages Receiving Slurry or Urea SO AGRONOMY JOURNAL LA English DT Article ID FALL-GROWN OAT; REPLACEMENT DAIRY HEIFERS; MANURE APPLICATION; NUTRITIVE-VALUE; RESIDUE LEVEL; PHOSPHORUS; CORN; RUNOFF; DATE; SOIL AB Although fall-grown oat (Avena sativa L.) has potential for use as supplementary fall forage throughout the north-central United States, one additional benefit is the option for summer manure distribution that is not possible within a conventional corn (Zea mays L.) cropping program. 'ForagePlus' oat was seeded into replicated plots fertilized with urea at rates of 0, 20, 40, 60, or 100 kg N ha(-1) or with dairy slurry applied at approximately 42,300 and 84,600 L ha-1 (105 and 209 kg N ha-1, respectively). Apparent N recovery from fall-grown oat plots fertilized with urea ranged from 65.8 to 89.8%. This suggested aggressive scavenging of N, but calculation of these high apparent N recovery rates was dependent on relatively low preplanting soil NO3-N concentrations, as well as a good dry matter (DM) yield response to N fertilization (y = -0.114x(2) + 29.9x + 2130; R-2 = 0.982). For this study, applications of high and low rates of dairy slurry yielded respective N fertilizer equivalencies of 38 and 45 kg N ha-1 for fall-grown oat, which are roughly equivalent to Wisconsin recommendations for cereal grains (45 kg N ha-1). Apparent N recovery following application of the high and low rates of dairy slurry was 18.1 and 25.5%, respectively; however, when expressed on the basis of NH4-N applied, apparent N recovery for fall-grown oat averaged 53.5% and did not differ among slurry application rate treatments. Fall-grown oat appears to be an effective scavenger of N applied as NH4-N from dairy slurry. C1 [Coblentz, Wayne; Jokela, William] ARS, USDA, US Dairy Forage Res Ctr, 2615 Yellowstone Dr, Marshfield, WI 54449 USA. [Cavadini, Jason S.] Univ Wisconsin, Marshfield Agr Res Stn, M605 Drake Ave, Stratford, WI 54484 USA. RP Coblentz, W (reprint author), ARS, USDA, US Dairy Forage Res Ctr, 2615 Yellowstone Dr, Marshfield, WI 54449 USA. EM wayne.coblentz@ars.usda.gov NR 34 TC 1 Z9 1 U1 1 U2 3 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2016 VL 108 IS 4 BP 1390 EP 1404 DI 10.2134/agronj2016.01.0009 PG 15 WC Agronomy SC Agriculture GA DR2HI UT WOS:000379725200008 ER PT J AU Tewolde, H Sistani, KR McLaughlin, MR AF Tewolde, Haile Sistani, Karamat R. McLaughlin, Michael R. TI Residual Effect of Poultry Litter Applications on No-Till Cotton Lint Yield SO AGRONOMY JOURNAL LA English DT Article ID BROILER LITTER; UPLAND SOIL; CONSERVATION TILLAGE; AMMONIUM-NITRATE; PHOSPHORUS; CORN; MOVEMENT; NITROGEN; ROTATION; MANURE AB In no-till cotton (Gossypium hirsutum L.) production systems, the potential residual benefits to lint yield from prior years of poultry litter applications are not well known. The objective of this study was to quantify the productivity of no-till cotton fertilized or unfertilized with synthetic N in years subsequent to the last poultry litter application. Treatments included 3- or 6-yr histories of 0, 2.2, 4.5, or 6.7 Mg ha(-1) yr(-1) litter; 4.5 Mg ha(-1) yr(-1) litter plus 67 kg ha(-1) yr(-1) N as urea-ammonium nitrate solution(UAN); and 118 or 135 kg ha(-1) yr(-1) UAN-N plus inorganic P and K as needed. In subsequent years, cotton was grown with residual from these treatments with or without 135 kg ha(-1) yr(-1) UAN-N. Cotton produced up to 25% more lint yield in the first year from the residual of 3- or 6-yr litter applications of 4.5 or 6.7 Mg ha(-1) yr(-1) relative to residual from synthetic fertilizers. The benefit of a 6-yr history of 4.5 or 6.7 Mg ha(-1) litter application, when followed by fertilization with 135 kg ha(-1) UAN-N, was 12% lint yield increase in the first year. The residual benefit persisted up to 5 yr but was greatest in the first year and diminished with time. The results overall show that including poultry litter as a component of the no-till cotton fertilization system can enhance cotton production not only in the year of application but also in subsequent years after stopping litter application and resuming conventional fertilization with synthetic fertilizers. C1 [Tewolde, Haile; McLaughlin, Michael R.] ARS, USDA, Crop Sci Res Lab, Mississippi State, MS 39762 USA. [Sistani, Karamat R.] ARS, USDA, Food Anim Environm Syst Res Unit, Bennett Lane, Bowling Green, KY 42104 USA. RP Tewolde, H (reprint author), ARS, USDA, Crop Sci Res Lab, Mississippi State, MS 39762 USA. EM haile.tewolde@ars.usda.gov NR 25 TC 1 Z9 1 U1 5 U2 6 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2016 VL 108 IS 4 BP 1405 EP 1414 DI 10.2134/agronj2016.01.0059 PG 10 WC Agronomy SC Agriculture GA DR2HI UT WOS:000379725200009 ER PT J AU Grey, TL Branch, WD Tubbs, RS Snider, JL Webster, TM Arnold, J Li, X AF Grey, Timothy L. Branch, William D. Tubbs, R. Scott Snider, John L. Webster, Theodore M. Arnold, Jason Li, Xiao TI The Impact of Genotype x Environment Effects on Runner-Type Peanut Seed Vigor Response to Temperature SO AGRONOMY JOURNAL LA English DT Article ID TOMATO-SPOTTED-WILT; ROOT-KNOT NEMATODE; BREEDING LINES; PHENOLOGICAL DEVELOPMENT; SOIL-TEMPERATURE; ARACHIS-HYPOGAEA; REGISTRATION; GERMINATION; EMERGENCE; RESISTANCE AB Experiments conducted from 2007 to 2012 evaluated the genotype x environment effects on breeder seed of eight peanut (Arachis hypogaea L.) cultivars grown under similar production practices. Seed germination and vigor by plot replication were evaluated in Petri-dishes incubated over a thermal gradient ranging from 12 to 36 degrees C at approximately 1.0 degrees C increments. Growing degree day (GDD) accumulation for each temperature increment was calculated based on daily mean temperature measured by thermocouples. Lorentzian distribution models were used to establish the temperature and time (hours) to maximum germination. With respect to maximum germination and temperature; Georgia-07W (23.9 degrees C) < Florida-07 (25.0 degrees C) < Georgia Greener (25.2 degrees C) < Georgia-06G (25.3 degrees C) = Tifguard < Georgia Green (26.4 degrees C) < Georgia-09B (27.1 degrees C) = Georgia-02C. Nonlinear regression indices from logistic growth curves with three parameters were used to elucidate seed germination by cultivar. These data included maximum indices of germination for each cultivar by year establishing parameter estimates using nonlinear regression, GDD value at 80% germination (Germ80), and temperature required by each cultivar for optimum germination. Peanut cultivar vigor varied by year with respect to overall GDDs to reach Germ80 and maximum germination (b(0)). Ranking of cultivars by GDD to reach Germ80 were: Georgia Green < Georgia-02C < Georgia-06G = Georgia Greener < Georgia-09B < Georgia-07W < Tifguard < Florida-07. This indicates that Georgia Green had much greater vigor than Florida-07 over the course of the experiments. Georgia-06G peanut seed were consistent with respect to germination, Germ(80), and b(0). All cultivars exhibited phenotypic variation by year, with genotype consistency across years. C1 [Grey, Timothy L.; Branch, William D.; Tubbs, R. Scott; Snider, John L.] Univ Georgia, Crop & Soil Sci Dept, Tifton Campus,Coastal Way, Tifton, GA 31793 USA. [Webster, Theodore M.] ARS, Crop Protect & Management Res Unit, USDA, POB 748, Tifton, GA 31793 USA. [Arnold, Jason] Pioneer Hibreed Int, 2300 Ind Pk Dr, Cairo, GA 39828 USA. [Li, Xiao] Auburn Univ, Dept Crop Soil & Environm Sci, Auburn, AL 36879 USA. RP Grey, TL (reprint author), Univ Georgia, Crop & Soil Sci Dept, Tifton Campus,Coastal Way, Tifton, GA 31793 USA. EM tgrey@uga.edu FU Georgia Peanut Commission; University of Georgia; USDA/ARS FX The authors would like to thank the Georgia Peanut Commission for partial funding of this project, the Georgia Seed Development Commission, and support from the University of Georgia and USDA/ARS. The authors also thank Sidney Cromer for technical assistance and Jerry Davis for statistical analysis. NR 59 TC 0 Z9 0 U1 8 U2 8 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2016 VL 108 IS 4 BP 1424 EP 1433 DI 10.2134/agronj2015.0420 PG 10 WC Agronomy SC Agriculture GA DR2HI UT WOS:000379725200011 ER PT J AU Long, DS McCallum, JD Martin, CT Capalbo, S AF Long, Dan S. McCallum, John D. Martin, Charles T. Capalbo, Susan TI Net Returns from Segregating Dark Northern Spring Wheat by Protein Concentration during Harvest SO AGRONOMY JOURNAL LA English DT Article ID GRAIN PROTEIN; QUALITY; YIELD AB In-line, optical sensing has been developed for on-combine measurement and mapping of grain protein concentration (GPC). The objective of this study was to estimate changes in costs and net returns from using this technology for segregation of the dark northern spring (DNS) subclass of hard red wheat (Triticum aestivum L.) by GPC. Site-specific GPC and yield data were obtained from 21 DNS wheat fields in northern Montana over 11 yr (1994-2004). Several hundred measurements of GPC were obtained within each field by means of whole grain spectroscopy. A calculator was built to predict the best economic point at which to segregate grain into two bins and define the protein level and quantity of each volume of grain. Partial budget analysis was used to estimate net returns and determine if segregating wheat was more profitable than bulking wheat in one bin. Segregation consistently increased the value of each megagram of grain if the GPC of a field was below the upper limit of a price schedule and also did not coincide with a price step. Added income was insufficient to offset costs of segregation based on 21-yr (1994-2014) average market prices. Costs could be offset in years when prices were sharply higher than average 21-yr prices. These results suggest that grain segregation may be profitable under certain conditions. Further refinements to the technique are needed to reduce equipment costs and benefit the producer. C1 [Long, Dan S.; McCallum, John D.] ARS, USDA, Soil & Water Conservat Res Unit, 48037 Tubbs Ranch Rd, Adams, OR 97810 USA. [Martin, Charles T.] Syngenta Seeds Inc, 1496 Boundary Rd, Grants Pass, OR 97527 USA. [Capalbo, Susan] Oregon State Univ, Appl Econ, 213 Ballard Extens Hall,2591 SW Campus Way, Corvallis, OR 97331 USA. RP Long, DS (reprint author), ARS, USDA, Soil & Water Conservat Res Unit, 48037 Tubbs Ranch Rd, Adams, OR 97810 USA. EM dan.long@ars.usda.gov NR 24 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2016 VL 108 IS 4 BP 1503 EP 1513 DI 10.2134/agronj2015.0457 PG 11 WC Agronomy SC Agriculture GA DR2HI UT WOS:000379725200020 ER PT J AU Anderson, W Parker, MB Knoll, JE Lacy, RC AF Anderson, William Parker, Myron B. Knoll, Joseph Edward Lacy, R. Curt TI Fertiliza ion Ratios of N-P2O5-K2O for Tifton 85 Bermudagrass on Two Coastal Plain Soils SO AGRONOMY JOURNAL LA English DT Article ID FORAGE AB Bermudagrass [Cynodon dactylon (L.) Pers.] is widely grown throughout the southeastern United States and many other countries for forage. Tifton 85, a hybrid between C. dactylon and C. nlemfuensis, is currently the recommended cultivar for grazing and hay. This study was conducted to determine the response of Tifton 85 to six rates of N fertilization (224, 336, 448, 560, 672, and 784 kg ha(-1)) and three rates of PK fertilization (50, 100, and 150% replacement of P and K removal) on two different soils, Carnegie (fine, kaolinitic, thermic Plinthic Kandiudult) and Fuquay (loamy, kaolinitic, thermic Arenic Plinthic Kandiudult), for 4 yr. In a randomized split-plot design, whole plots were N rates with PK rates randomized within whole plots. The optimal fertilization rate to provide maximum profits was determined. Though yields began to level off only at the 560 kg ha(-1) N application rate, rates from 224 to 448 kg ha(-1) N with 100% replacement of P and K uptake resulted in maximum economic return. At these N rates, based on actual nutrient uptake, the N-P2O5-K2O ratio for fertilization of Tifton 85 should be approximately 4-1-5. At 336 to 448 kg ha1 N, a grower could expect approximately 19.6 to 23.0 Mg ha(-1) yr(-1) hay at 150 g kg(-1) moisture. Crude protein (CP) concentration and in vitro dry matter digestibility (IVDMD) of the harvested forage both responded positively to increasing rates of N application beyond the economically optimum rates. C1 [Anderson, William; Knoll, Joseph Edward] ARS, USDA, Crop Genet & Breeding Res Unit, POB 748, Tifton, GA 31794 USA. [Parker, Myron B.] Univ Georgia, Crop & Soils, 115 Coastal Way, Tifton, GA 31793 USA. [Lacy, R. Curt] Mississippi State Univ Extens, 82 Stoneville Rd POB 197, Stoneville, MS 38776 USA. RP Anderson, W (reprint author), ARS, USDA, Crop Genet & Breeding Res Unit, POB 748, Tifton, GA 31794 USA. EM bill.anderson@ars.usda.gov NR 15 TC 0 Z9 0 U1 1 U2 3 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2016 VL 108 IS 4 BP 1542 EP 1551 DI 10.2134/agronj2015.0585 PG 10 WC Agronomy SC Agriculture GA DR2HI UT WOS:000379725200024 ER PT J AU Sindelar, AJ Schmer, MR Jin, VL Wienhold, BJ Varvel, GE AF Sindelar, Aaron J. Schmer, Marty R. Jin, Virginia L. Wienhold, Brian J. Varvel, Gary E. TI Crop Rotation Affects Corn, Grain Sorghum, and Soybean Yields and Nitrogen Recovery SO AGRONOMY JOURNAL LA English DT Article ID LONG-TERM; FERTILIZER RECOVERY; USE EFFICIENCY; AGRONOMIC PERFORMANCE; MAIZE PRODUCTION; GREAT-PLAINS; N-2 FIXATION; N CREDIT; SYSTEMS; SOIL AB Long-term cropping system and fertilizer N studies are essential to understanding production potential and yield stability of corn (Zea mays L.), grain sorghum [Sorghum bicolor (L.) Moench], and soybean [Glycine max (L.) Merr.] in rain-fed environments. A no-till experiment (2007-2013) was conducted in eastern Nebraska to evaluate crop rotation (continuous corn, continuous grain sorghum, continuous soybean, corn-soybean, grain sorghum-soybean, corn-soybean-grain sorghum-oat [Avena sativa (L.)]/clover mixture [80% Melilotus officinalis Lam. + 20% Trifolium pretense L.], and corn-oat/clover-grain sorghum-soybean) and fertilizer N (corn and grain sorghum: 0, 90, 180 kg N ha(-1); soybean and oat/clover: 0, 36, 67 kg N ha(-1)) on grain yield, plant N uptake, and N recovery efficiency. Diversified crop rotations increased corn and grain sorghum yields and improved yield stability. A positive corn grain yield response to fertilizer N was consistent across crop rotations, but fertilizer N addition with corn-soybean-grain sorghum-oat did not increase grain sorghum yield. Yield stability of soybean was less sensitive to management; all treatment combinations were found to be stable. Fertilizer N addition decreased soybean grain yield in 2 of 7 yr, but yields were similar in the remaining 5 yr. These results indicate that adoption of 2- and 4-yr crop rotations in rain-fed environments can result in high-yielding, more stable corn, grain sorghum, and soybean grain production compared with shorter rotations or continuous cropping. C1 [Sindelar, Aaron J.; Schmer, Marty R.; Jin, Virginia L.; Wienhold, Brian J.; Varvel, Gary E.] Univ Nebraska, ARS, USDA, Agroecosyst Management Res Unit, 251 Filley Hall Food Ind Complex,East Campus, Lincoln, NE 68583 USA. RP Schmer, MR (reprint author), Univ Nebraska, ARS, USDA, Agroecosyst Management Res Unit, 251 Filley Hall Food Ind Complex,East Campus, Lincoln, NE 68583 USA. EM marty.schmer@ars.usda.gov FU Agriculture Research Service under the Greenhouse Gas Reduction through Agricultural Carbon Enhancement network (GRACEnet) FX This study was supported by the Agriculture Research Service under the Greenhouse Gas Reduction through Agricultural Carbon Enhancement network (GRACEnet). Mention of commercial names does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer. NR 56 TC 0 Z9 0 U1 16 U2 22 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2016 VL 108 IS 4 BP 1592 EP 1602 DI 10.2134/agronj2016.01.0005 PG 11 WC Agronomy SC Agriculture GA DR2HI UT WOS:000379725200029 ER PT J AU Simmons, JR Sistani, KR Pote, DH Ritchey, EL Jn-Baptiste, M Tewolde, H AF Simmons, Jason R. Sistani, Karamat R. Pote, Daniel H. Ritchey, Edwin L. Jn-Baptiste, Marcia Tewolde, Haile TI Corn Response and Soil Nutrient Concentration from Subsurface Application of Poultry Litter SO AGRONOMY JOURNAL LA English DT Article ID BROILER LITTER; AMMONIA VOLATILIZATION; NO-TILL; CATTLE SLURRY; COTTON; NITROGEN; MANURE; PASTURE; NITRATE; IMPACT AB Nitrogen fertilizer management is vital to corn (Zea mays L.) production from financial and environmental perspectives. Poultry litter as a nutrient source in this cropping system is generally surface broadcast, potentially causing volatilization of NH3. Recently a new application method was developed allowing subsurface banding of poultry litter with minimal soil surface disturbance. However, there are limited data with this application method on corn production. In this study, we used farm-scale plots (7.6 by 91.2 m) to evaluate the response of no-till corn growth and post-harvest soil nutrient concentrations from subsurface poultry litter application. All treatments were applied pre-plant at a rate of 168 kg N ha(-1) and included (i) a standard commercial fertilizer surface broadcast, (ii) poultry litter surface applied, and (iii) poultry litter applied in subsurface bands placed 30 cm apart and 8-cm deep below the soil surface. Results showed that poultry litter subsurface banded plots resulted in corn grain and above ground biomass yields similar to plots treated with commercial fertilizer. In 2011, corn grain yield of 11.35 Mg ha(-1) produced by the subsurface banded poultry litter treatment was significantly higher when compared to surface applied poultry litter that yielded 10.10 Mg ha(-1). Poultry litter application method did not have a significant effect on post-harvest soil pH, total C, total N, NH4(+)-N, NO3-N, P, and K concentrations. Results from this study suggest that subsurface banding of poultry litter can be utilized as an alternate application method in no-till corn without detrimental impacts on productivity. C1 [Simmons, Jason R.; Sistani, Karamat R.; Jn-Baptiste, Marcia] ARS, USDA, Food Anim Environm Syst Res Unit, 2413 Nashville Rd B5, Bowling Green, KY 42101 USA. [Pote, Daniel H.] ARS, USDA, Dale Bumpers Small Farms Res Ctr, Booneville, AR 72927 USA. [Ritchey, Edwin L.] Univ Kentucky, Dept Plant & Soil Sci, Res & Educ Ctr, 1205 Hopkinsville St, Princeton, KY 40546 USA. [Tewolde, Haile] ARS, USDA, Genet & Precis Agr Res Unit, Mississippi State, MS 39762 USA. RP Simmons, JR (reprint author), ARS, USDA, Food Anim Environm Syst Res Unit, 2413 Nashville Rd B5, Bowling Green, KY 42101 USA. EM jason.simmons@ars.usda.gov NR 34 TC 0 Z9 0 U1 10 U2 15 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2016 VL 108 IS 4 BP 1674 EP 1680 DI 10.2134/agronj2015.0529 PG 7 WC Agronomy SC Agriculture GA DR2HI UT WOS:000379725200037 ER PT J AU Logsdon, SD Sauer, PA AF Logsdon, S. D. Sauer, P. A. TI Iron Filings Cement Engineered Soil Mix SO AGRONOMY JOURNAL LA English DT Article ID SULFIDE MINE TAILINGS; LAYERS; PHOSPHORUS; MANGANESE; PERFORMANCE; COPPER; SAND; ZINC; USA AB Engineered soil mixes are expected to maintain plant growth while minimizing nutrient leaching in greenhouse and bioretention applications. Iron (Fe) has been suggested within plant growth mixtures as a sorbent for phosphorus. The purpose of this study was to determine if Fe filings are an appropriate component of engineered plant growth mixtures. Iron filings were added to engineered soil mixes in laboratory columns, and plants were grown for 2 mo. Unfortunately, Fe filings partially cemented the engineered mixes, especially the treatment with sand and compost but no soil. Aft er completing the study, orange and red aggregates were apparent. Although Fe filings might be useful in filter beds, they were not suitable within engineered plant growth mixes. C1 [Logsdon, S. D.] USDA ARS, NLAE, 1015 N Univ Blvd, Ames, IA 50011 USA. [Sauer, P. A.] Iowa Stormwater Educ Partnership, POB 1826, Ames, IA 50010 USA. RP Logsdon, SD (reprint author), USDA ARS, NLAE, 1015 N Univ Blvd, Ames, IA 50011 USA. EM sally.logsdon@ars.usda.gov FU Leopold Center for Sustainable Agriculture FX This study was funded by the Leopold Center for Sustainable Agriculture. The authors thank Gavin Simmons for experimental work; Amy Morrow for analytical lab support and interpretation; John Kovar, Andrew Erickson, and Jerry Hatfield for helpful comments on earlier versions of the manuscript; the anonymous reviewers who provided helpful comments for clarification; and Dean Mattoon, Carol Sweeting, Dr. Art Bettis, Brian Stineman, and Mark Augustine for coordinating sites to collect urban soil. NR 32 TC 0 Z9 0 U1 6 U2 12 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2016 VL 108 IS 4 BP 1753 EP 1757 DI 10.2134/agronj2015.0427 PG 5 WC Agronomy SC Agriculture GA DR2HI UT WOS:000379725200045 ER PT J AU Arismendi, I Penaluna, BE AF Arismendi, Ivan Penaluna, Brooke E. TI Examining Diversity Inequities in Fisheries Science: A Call to Action SO BIOSCIENCE LA English DT Article DE education; tenure track; environmental sciences; aquaculture; academia ID TEAMS AB A diverse workforce in science can bring about competitive advantages, innovation, and new knowledge, skills, and experiences for understanding complex problems involving the science and management of natural resources. In particular, fisheries sciences confronts exceptional challenges because of complicated societal-level problems from the overexploitation and degradation of aquatic ecosystems worldwide. Here, we examine the status of gender and race or ethnicity among the US fisheries science workforce on the basis of a survey of 498 faculty members from 56 institutions of higher education and 1717 federal employees. Our findings show that women and minorities are still a small portion of tenure-track faculty and federal-government professionals, likely because of systemic biases and cultural barriers. This forum provides a starting point for discussions about how the disparities of diversity in fisheries compares with other disciplines and what might be done to improve the climate and conditions for the successful inclusion of diverse scientists. C1 [Arismendi, Ivan] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Penaluna, Brooke E.] US Forest Serv, USDA, Pacific Northwest Res Stn, Corvallis, OR USA. RP Arismendi, I (reprint author), Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. EM ivan.arismendi@oregonstate.edu RI Arismendi, Ivan/B-3144-2010 OI Arismendi, Ivan/0000-0002-8774-9350 FU US Forest Service, Pacific Northwest Research Station FX Officials from the Freedom of Information Act Request (FOIA) and American Fisheries Society provided quick and favorable responses to data set queries. Eric Hicklin helped with data collection. Kathryn Ronnenberg assisted with figure art composition and Kelly Christiansen prepared the map. Jennifer Almquist, Michael Nelson, Rachel White, Regan Penaluna, Sherri Johnson, Rebecca Flitcroft, Deanna Olson, Samuel Schmieding provided discussion on early versions of the manuscript, which was supported by the US Forest Service, Pacific Northwest Research Station. Two anonymous reviewers and the editor provided insightful comments and suggestions. NR 35 TC 0 Z9 0 U1 7 U2 7 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0006-3568 EI 1525-3244 J9 BIOSCIENCE JI Bioscience PD JUL PY 2016 VL 66 IS 7 BP 584 EP 591 DI 10.1093/biosci/biw041 PG 8 WC Biology SC Life Sciences & Biomedicine - Other Topics GA DR7JD UT WOS:000380075000009 ER PT J AU Schipanski, ME MacDonald, GK Rosenzweig, S Chappell, MJ Bennett, EM Kerr, RB Blesh, J Crews, T Drinkwater, L Lundgren, JG Schnarr, C AF Schipanski, Meagan E. MacDonald, Graham K. Rosenzweig, Steven Chappell, M. Jahi Bennett, Elena M. Kerr, Rachel Bezner Blesh, Jennifer Crews, Timothy Drinkwater, Laurie Lundgren, Jonathan G. Schnarr, Cassandra TI Realizing Resilient Food Systems SO BIOSCIENCE LA English DT Article DE agroecology; global change; food security; human nutrition; social-ecological systems ID BIODIVERSITY; MANAGEMENT; NUTRITION; SECURITY; AGROECOSYSTEMS; PERSPECTIVE; RETHINKING; VEGETATION; LEVERAGE; BENEFIT AB Food systems are under increasing pressure to produce sufficient food for the global population, decrease the environmental impacts of production, and buffer against complex global change. Food security also remains elusive for many populations worldwide. Greater emphasis on food system resilience could reduce these vulnerabilities. We outline integrated strategies that together could foster food system resilience across scales, including (a) integrating gender equity and social justice into food security research and initiatives, (b) increasing the use of ecological processes rather than external inputs for crop production, (c) fostering regionalized food distribution networks and waste reduction, and (d) linking human nutrition and agricultural production policies. Enhancing social-ecological links and fostering adaptive capacity are essential to cope with short-term volatility and longer-term global change pressures. Finally, we highlight regional case studies that have enhanced food system resilience for vulnerable populations. Efforts in these areas could have dramatic impacts on global food system resilience. C1 [Schipanski, Meagan E.; Rosenzweig, Steven; Schnarr, Cassandra] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [MacDonald, Graham K.] McGill Univ, Dept Geog, Montreal, PQ, Canada. [MacDonald, Graham K.] Univ Minnesota, Inst Environm, St Paul, MN 55108 USA. [Chappell, M. Jahi] Inst Agr & Trade Policy, Minneapolis, MN USA. [Bennett, Elena M.] McGill Univ, Dept Nat Resource Sci, Ste Anne De Bellevue, PQ, Canada. [Bennett, Elena M.] McGill Univ, McGill Sch Environm, Montreal, PQ, Canada. [Kerr, Rachel Bezner] Cornell Univ, Dept Dev Sociol, Ithaca, NY USA. [Blesh, Jennifer] Univ Michigan, Sch Nat Resources & Environm, Ann Arbor, MI 48109 USA. [Crews, Timothy] Land Inst, Salina, KS USA. [Drinkwater, Laurie] Cornell Univ, Dept Hort, Ithaca, NY USA. [Lundgren, Jonathan G.] ARS, North Cent Agr Res Lab, USDA, Brookings, SD USA. RP Schipanski, ME (reprint author), Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. EM meagan.schipanski@colostate.edu RI Chappell, M. Jahi/E-9398-2010 OI Chappell, M. Jahi/0000-0001-8542-1610 FU USDA NIFA [2012-67012-22889]; CSU School of Global and Environmental Sustainability; Natural Sciences and Engineering Research Council of Canada (NSERC); Gordon and Betty Moore Foundation FX M. Schipanski and G. MacDonald shared leadership in writing the manuscript. We would like to thank Ricardo Salvador, David Tilman, and Jonathan Foley for their contributions to the Ignite Session at the Ecological Society of America meeting that inspired this manuscript. Theresa Nogeire provided valuable feedback on the manuscript. Funding for M. Schipanski was provided by the USDA NIFA (no. 2012-67012-22889) and the CSU School of Global and Environmental Sustainability. G. MacDonald was supported through the Natural Sciences and Engineering Research Council of Canada (NSERC) and the Gordon and Betty Moore Foundation. NR 59 TC 1 Z9 1 U1 31 U2 46 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0006-3568 EI 1525-3244 J9 BIOSCIENCE JI Bioscience PD JUL PY 2016 VL 66 IS 7 BP 600 EP 610 DI 10.1093/biosci/biw052 PG 11 WC Biology SC Life Sciences & Biomedicine - Other Topics GA DR7JD UT WOS:000380075000011 ER PT J AU Roffler, GH Schwartz, MK Pilgrim, KL Talbot, SL Sage, GK Adams, LG Luikart, G AF Roffler, Gretchen H. Schwartz, Michael K. Pilgrim, Kristy L. Talbot, Sandra L. Sage, George K. Adams, Layne G. Luikart, Gordon TI Identification of landscape features influencing gene flow: How useful are habitat selection models? SO EVOLUTIONARY APPLICATIONS LA English DT Article DE dispersal; landscape genetics; multiple regression on distance matrices; Ovis dalli dalli; population connectivity; resistance surfaces; resource selection function ID POPULATION-STRUCTURE; NEIGHBOR MATRICES; SPATIAL-ANALYSIS; ECOLOGICAL DATA; CONNECTIVITY; INFORMATION; DISPERSAL; INFERENCE; RESISTANCE; FRAMEWORK AB Understanding how dispersal patterns are influenced by landscape heterogeneity is critical for modeling species connectivity. Resource selection function (RSF) models are increasingly used in landscape genetics approaches. However, because the ecological factors that drive habitat selection may be different from those influencing dispersal and gene flow, it is important to consider explicit assumptions and spatial scales of measurement. We calculated pairwise genetic distance among 301 Dall's sheep (Ovis dalli dalli) in southcentral Alaska using an intensive noninvasive sampling effort and 15 microsatellite loci. We used multiple regression of distance matrices to assess the correlation of pairwise genetic distance and landscape resistance derived from an RSF, and combinations of landscape features hypothesized to influence dispersal. Dall's sheep gene flow was positively correlated with steep slopes, moderate peak normalized difference vegetation indices (NDVI), and open land cover. Whereas RSF covariates were significant in predicting genetic distance, the RSF model itself was not significantly correlated with Dall's sheep gene flow, suggesting that certain habitat features important during summer (rugged terrain, mid-range elevation) were not influential to effective dispersal. This work underscores that consideration of both habitat selection and landscape genetics models may be useful in developing management strategies to both meet the immediate survival of a species and allow for long-term genetic connectivity. C1 [Roffler, Gretchen H.; Talbot, Sandra L.; Sage, George K.; Adams, Layne G.] US Geol Survey, Alaska Sci Ctr, Anchorage, AK USA. [Roffler, Gretchen H.; Schwartz, Michael K.] Univ Montana, Coll Forestry & Conservat, Wildlife Biol Program, Dept Ecosyst Sci & Conservat, Missoula, MT 59812 USA. [Schwartz, Michael K.; Pilgrim, Kristy L.] US Forest Serv, Rocky Mt Res Stn, Missoula, MT USA. [Luikart, Gordon] Univ Montana, Flathead Lake Biol Stn, Polson, MT 59860 USA. [Luikart, Gordon] Univ Montana, Div Biol Sci, Fish & Wildlife Genom Grp, Missoula, MT 59812 USA. [Roffler, Gretchen H.] Alaska Dept Fish & Game, Div Wildlife Conservat, 802 3rd St, Douglas, AK 99824 USA. RP Roffler, GH (reprint author), Alaska Dept Fish & Game, Div Wildlife Conservat, 802 3rd St, Douglas, AK 99824 USA. EM gretchen.roffler@alaska.gov OI Roffler, Gretchen/0000-0002-8534-3664 FU National Park Service (NPS); US Geological Survey (USGS); USGS; University of Montana; NSF [DEB 1258203, 1067613] FX This work was funded by the National Park Service (NPS) and the US Geological Survey (USGS). The NPS and the Alaska Department of Fish and Game (ADF&G) provided survey data and valuable insights. For fieldwork assistance, we thank T. Cambier, H. McMahan, J. Putera, and R. Schwanke. We are grateful to ADF&G for collecting genetic samples. We especially thank J. Tucker and M. Elis for analytical assistance and B. McRae, T. Mohapatra, and V. Shah for computational support. B. Hand and M. Hebblewhite provided useful comments on early drafts of the manuscript. G.R. was supported by the USGS and the University of Montana. G.L. was supported by NSF grants DEB 1258203 and 1067613. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 67 TC 0 Z9 0 U1 13 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1752-4571 J9 EVOL APPL JI Evol. Appl. PD JUL PY 2016 VL 9 IS 6 BP 805 EP 817 DI 10.1111/eva.12389 PG 13 WC Evolutionary Biology SC Evolutionary Biology GA DR5GQ UT WOS:000379931900006 PM 27330556 ER PT J AU McHugh, T AF McHugh, Tara TI How Hummus Is Processed SO FOOD TECHNOLOGY LA English DT Article C1 [McHugh, Tara] ARS, USDA, Albany, CA 94710 USA. RP McHugh, T (reprint author), ARS, USDA, Albany, CA 94710 USA. EM tara.mchugh@ars.usda.gov NR 0 TC 0 Z9 0 U1 4 U2 4 PU INST FOOD TECHNOLOGISTS PI CHICAGO PA 525 WEST VAN BUREN, STE 1000, CHICAGO, IL 60607-3814 USA SN 0015-6639 J9 FOOD TECHNOL-CHICAGO JI Food Technol. PD JUL PY 2016 VL 70 IS 7 BP 91 EP 94 PG 4 WC Food Science & Technology SC Food Science & Technology GA DR4QP UT WOS:000379887600020 ER PT J AU Guard, J Abdo, Z Byers, SO Kriebel, P Rothrock, MJ AF Guard, Jean Abdo, Zaid Byers, Sara Overstreet Kriebel, Patrick Rothrock, Michael J., Jr. TI Subtyping of Salmonella enterica Subspecies I Using Single-Nucleotide Polymorphisms in Adenylate Cyclase SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID LISTERIA-MONOCYTOGENES; SEROVAR TYPHIMURIUM; GENETIC DIVERSITY; VIRULENCE GENES; SEQUENCE; ENTERITIDIS; STRAINS; EVOLUTIONARY; MUTATIONS; SEROTYPE AB Methods to rapidly identify serotypes of Salmonella enterica subspecies I are of vital importance for protecting the safety of food. To supplement the serotyping method dkgB-linked intergenic sequence ribotyping (ISR), single-nucleotide polymorphisms were characterized within adenylate cyclase (cyaA). The National Center for Biotechnology Information (NCBI) database had 378 cyaA sequences from S. enterica subspecies I, which included 42 unique DNA sequences and 19 different amino acid sequences. Five representative isolates, namely serotypes Typhimurium, Kentucky, Enteritidis phage type PT4, and two variants of Enteritidis phage type PT13a, were differentiated within a microsphere-based fluidics system in cyaA by allele-specific primer extension. Validation against 25 poultry-related environmental Salmonella isolates representing 11 serotypes yielded a similar to 89% success rate at identifying the serotype of the isolate, and a different region could be targeted to achieve 100%. When coupled with ISR, all serotypes were differentiated. Phage lineages of serotype Enteritidis 13a and 4 were identified, and a biofilm-forming strain of PT13a was differentiated from a smooth phenotype within phage type. Comparative ranking of mutation indices to genes such as the tRNA transferases, the diguanylate cyclases, and genes used for multilocus sequence typing indicated that cyaA is an appropriate gene for assessing epidemiological trends of Salmonella because of its relative stability in nucleotide composition. C1 [Guard, Jean; Abdo, Zaid; Rothrock, Michael J., Jr.] USDA, US Natl Poultry Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. [Byers, Sara Overstreet; Kriebel, Patrick] Univ Georgia, Dept Stat, Athens, GA 30602 USA. RP Guard, J (reprint author), USDA, US Natl Poultry Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. EM jean.guard@ars.usda.gov RI Abdo, Zaid/E-5176-2017 OI Abdo, Zaid/0000-0002-8272-7734 FU U.S. Poultry and Egg Association Foundation [F043]; Agricultural Research Service, USDA CRIS [6612-41420-017-00, 6612-32000-007-00] FX The authors would like to acknowledge the expert technical assistance of Laura Lee-Rutherford in the development of primer sets and optimization of SNP assays, and Tod Stewart for assistance with the operation and maintenance of the MagPix System. These investigations were supported through the U.S. Poultry and Egg Association Foundation Project No. F043 as well as additional support from Agricultural Research Service, USDA CRIS Projects "Pathogen Reduction and Processing Parameters in Poultry Processing Systems" No. 6612-41420-017-00 and "Genetic Analysis of Poultry-Associated Salmonella enterica to Identify and Characterize Properties and Markers Associated with Egg-Borne Transmission of Illness" No. 6612-32000-007-00. NR 57 TC 0 Z9 0 U1 3 U2 4 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 EI 1556-7125 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD JUL PY 2016 VL 13 IS 7 BP 350 EP 362 DI 10.1089/fpd.2015.2088 PG 13 WC Food Science & Technology SC Food Science & Technology GA DQ8GR UT WOS:000379447600002 PM 27035032 ER PT J AU Feng, G Cobb, S Abdo, Z Fisher, DK Ouyang, Y Adeli, A Jenkins, JN AF Feng, Gary Cobb, Stacy Abdo, Zaid Fisher, Daniel K. Ouyang, Ying Adeli, Ardeshir Jenkins, Johnie N. TI Trend Analysis and Forecast of Precipitation, Reference Evapotranspiration, and Rainfall Deficit in the Blackland Prairie of Eastern Mississippi SO JOURNAL OF APPLIED METEOROLOGY AND CLIMATOLOGY LA English DT Article ID ARIDITY INDEX; CLIMATE MODEL; UNITED-STATES; VARIABILITY; SIMULATIONS; REGRESSION AB Trend analysis and estimation of monthly and annual precipitation, reference evapotranspiration ETo, and rainfall deficit are essential for water-resources management and cropping-system design. Rainfall, ETo, and water-deficit patterns and trends at Macon in eastern Mississippi for a 120-yr period (1894-2014) were analyzed for annual, seasonal, and monthly periods. The analysis showed historical average annual rainfall, ETo, and dryness index (DI) in the location to be 1307 mm, 1210 mm, and 0.97, respectively. Monthly rainfall and ETo ranged from 72 to 118 mm and from 94 to 146 mm, respectively, between May and October, resulting in a monthly rain deficit from 22 to 62 mm. Annual rainfall showed an increasing trend of 1.17 mm yr(-1) while annual ETo exhibited a decreasing trend of -0.51 mm yr(-1), resulting in an annual DI reduction of 0.001 per year. Seasonal trends were found for rainfall in autumn (1.06 mm yr (1)), ETo in summer (-0.29 mm yr (1)) and autumn (-0.18 mm yr(-1)), and DI in autumn (-0.006). An autoregressive, integrated, and moving-average (ARIMA) approach was used to model monthly and annual rainfall, ETo, and DI and to predict those values in the future. Low values of the root-mean-square error (RMSE) and mean absolute error (with both statistics being normalized to the mean of the observed values), low values of average percent bias, and low values of the ratio of the RMSE to the standard deviation of observed data, along with values of 1.0 for Nash-Sutcliffe modeling efficiency and the index of agreement, all suggest that the performance of the models is acceptable. The ARIMA models forecast 1319 mm of mean annual rainfall, 1203 mm of mean annual ETo, and 0.82 of mean annual DI from 2015 to 2024. The results obtained from this research can guide development of water-management practices and cropping systems in the area that rely on this weather station. The approaches used and the models fitted in this study can serve as a demonstration of how a time series trend can be analyzed and a model fitted at other locations. C1 [Feng, Gary; Adeli, Ardeshir; Jenkins, Johnie N.] USDA ARS, Mississippi State, MS USA. [Cobb, Stacy] Univ Georgia, Athens, GA 30602 USA. [Abdo, Zaid] USDA ARS, Athens, GA 30613 USA. [Fisher, Daniel K.] USDA ARS, Stoneville, MS 38776 USA. [Ouyang, Ying] US Forest Serv, USDA, Mississippi State, MS USA. [Abdo, Zaid] Colorado State Univ, Ft Collins, CO 80523 USA. RP Feng, G (reprint author), USDA ARS, Sustainable Agr Res Unit, 810 Highway 12 East, Mississippi State, MS 39762 USA. EM gary.feng@ars.usda.gov RI Abdo, Zaid/E-5176-2017 OI Abdo, Zaid/0000-0002-8272-7734 FU Mississippi Soybean Promotion Board [62-2015] FX This project (62-2015) was funded by the Mississippi Soybean Promotion Board. NR 44 TC 2 Z9 2 U1 5 U2 7 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 1558-8424 EI 1558-8432 J9 J APPL METEOROL CLIM JI J. Appl. Meteorol. Climatol. PD JUL PY 2016 VL 55 IS 7 BP 1425 EP 1439 DI 10.1175/JAMC-D-15-0265.1 PG 15 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DR3SP UT WOS:000379823200001 ER PT J AU Ono-Moore, KD Snodgrass, RG Huang, SR Singh, S Freytag, TL Burnett, DJ Bonnel, EL Woodhouse, LR Zunino, SJ Peerson, JM Lee, JY Rutledge, JC Hwang, DH AF Ono-Moore, Kikumi D. Snodgrass, Ryan G. Huang, Shurong Singh, Shamsher Freytag, Tammy L. Burnett, Dustin J. Bonnel, Ellen L. Woodhouse, Leslie R. Zunino, Susan J. Peerson, Janet M. Lee, Joo Young Rutledge, John C. Hwang, Daniel H. TI Postprandial Inflammatory Responses and Free Fatty Acids in Plasma of Adults Who Consumed a Moderately High-Fat Breakfast with and without Blueberry Powder in a Randomized Placebo-Controlled Trial SO JOURNAL OF NUTRITION LA English DT Article DE diet and dietary lipids; antioxidants; postprandial lipemia; plasma free fatty acids; postprandial inflammation; lipoprotein lipase; cytokines; monocyte activation; blueberries ID RECEPTOR-MEDIATED INFLAMMATION; TOLL-LIKE RECEPTOR-4; DIET-INDUCED OBESITY; SIGNALING PATHWAYS; DIFFERENTIAL MODULATION; INSULIN-RESISTANCE; HUMAN MONOCYTES; INHIBITION; MEN; MACROPHAGES AB Background: Saturated fatty acids (FAs) released from triglyceride-rich lipoproteins (TGRLs) activate Toll-like receptor 2 (TLR-2) and induce the expression of proinflammatory cytokines in monocytes. Certain plant polyphenols inhibit TLR-mediated signaling pathways. Objective: We determined whether plasma free FAs (FFAs) after a moderately high-fat (MHF, 40% kcal from fat) breakfast modulate the inflammatory status of postprandial blood, and whether blueberry intake suppresses FFA-induced inflammatory responses in healthy humans. Methods: Twenty-three volunteers with a mean SEM age and body mass index (in kg/m(2)) of 30 +/- 3 y and 21.9 +/- 0.4, respectively, consumed an MHF breakfast with either a placebo powder or 2 or 4 servings of blueberry powder in a randomized crossover design. The placebo powder was provided on the first test day and the blueberry powder doses were randomized with a 2-wk washout period. Plasma concentrations of lipids, glucose, and cytokines were determined. To determine whether FFAs derived from TGRL stimulate monocyte activation, and whether this is inhibited by blueberry intake, whole blood was treated with lipoprotein lipase (LPL). Results: The median concentrations of FFAs and cytokines [tumor necrosis factor-alpha, interleukin (IL)-6 and IL-8] in postprandial plasma (3.5 h) decreased compared with fasting plasma regardless of the blueberry intake (P < 0.001 for FFAs and P < 0.05 for cytokines). However, concentrations of FFAs and cytokines including IL-1 beta increased in LPL-treated whole blood compared with untreated blood samples from participants who consumed the placebo powder. Blueberry intake suppressed IL-1 beta and IL-6 production in LPL-treated postprandial blood compared with the placebo control when fasting changes were used as a covariate. Conclusions: The plasma FFA concentration may be an important determinant affecting inflammatory cytokine production in blood. Supplementation with blueberry powder did not affect plasma FFA and cytokine concentrations; however, it attenuated the cytokine production induced by ex vivo treatment of whole blood with LPL. This trial was registered at clinicaltrials.gov as NCT01594008. C1 [Ono-Moore, Kikumi D.; Snodgrass, Ryan G.; Huang, Shurong; Singh, Shamsher; Freytag, Tammy L.; Burnett, Dustin J.; Woodhouse, Leslie R.; Zunino, Susan J.; Peerson, Janet M.; Hwang, Daniel H.] ARS, Western Human Nutr Res Ctr, USDA, Davis, CA 95616 USA. [Ono-Moore, Kikumi D.; Snodgrass, Ryan G.; Bonnel, Ellen L.; Zunino, Susan J.; Peerson, Janet M.; Hwang, Daniel H.] Univ Calif Davis, Sch Med, Dept Nutr, Davis, CA 95616 USA. [Rutledge, John C.] Univ Calif Davis, Sch Med, Dept Internal Med, Davis, CA 95616 USA. [Lee, Joo Young] Catholic Univ Korea, Coll Pharm, Bucheon, South Korea. RP Hwang, DH (reprint author), ARS, Western Human Nutr Res Ctr, USDA, Davis, CA 95616 USA.; Hwang, DH (reprint author), Univ Calif Davis, Sch Med, Dept Nutr, Davis, CA 95616 USA. EM daniel.hwang@ars.usda.gov FU US Highbush Blueberry Council; USDA; Agricultural Research Service; Western Human Nutrition Research Center project funds [5306-51530-017-00D, 5306-51530- 018-00D]; USDA/NIFA [2013-03477]; NIH [R01AG045541] FX Supported in part by a grant from the US Highbush Blueberry Council and USDA, Agricultural Research Service, Western Human Nutrition Research Center project funds (5306-51530-017-00D to DHH; 5306-51530- 018-00D to SJZ), USDA/NIFA competitive grant (2013-03477 for DHH), and NIH (R01AG045541 to JCR). NR 49 TC 0 Z9 0 U1 5 U2 7 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD JUL PY 2016 VL 146 IS 7 BP 1411 EP 1419 DI 10.3945/jn.115.223909 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DQ7IL UT WOS:000379380100017 PM 27306892 ER PT J AU Hamby, KA Bellamy, DE Chiu, JC Lee, JC Walton, VM Wiman, NG York, RM Biondi, A AF Hamby, Kelly A. Bellamy, David E. Chiu, Joanna C. Lee, Jana C. Walton, Vaughn M. Wiman, Nik G. York, Riki M. Biondi, Antonio TI Biotic and abiotic factors impacting development, behavior, phenology, and reproductive biology of Drosophila suzukii SO JOURNAL OF PEST SCIENCE LA English DT Review DE Invasive species; Chronobiology; Development; Overwintering; Reproduction; Spotted wing drosophila ID SPOTTED-WING DROSOPHILA; DIPTERA DROSOPHILIDAE; FRUIT PRODUCTION; UNITED-STATES; PARASITOIDS ATTACKING; PRODUCTION REGIONS; SPECIES GROUP; MELANOGASTER; PEST; HOST AB Spotted wing drosophila, Drosophila suzukii, is a devastating invasive pest of small and stone fruits in the Americas and Europe. To better understand the population dynamics of D. suzukii, we reviewed recent work on juvenile development, adult reproduction, and seasonal variation in life history parameters including the abiotic/biotic factors that influence these processes. Juvenile development is optimal at moderately warm temperatures, and larvae exhibit some immunity to parasitism. Adults use visual cues and substrate-borne vibrations for courtship and exhibit a bimodal locomotor activity pattern (except mated females). Under 20-27 A degrees C and various conditions, development from egg to adult can take 10-17 days, females first lay eggs within 1-8 days and their lifetime fecundity varies from < 100 to > 400. Oviposition is consistently high in raspberry hosts and fruits with lower penetration force, and the presence of Wolbachia endosymbionts can lower fertility. Drosophila suzukii exhibit seasonal variation with a darker winter morph that is more cold tolerant. Also, D. suzukii likely undergo reproductive diapause in the fall, with colder temperatures and shorter day lengths influencing reproduction. To develop viable IPM programs for D. suzukii, knowledge of abiotic and biotic conditions that impact D. suzukii life history parameters and population dynamics is critical, and gaps in the current knowledge are discussed. C1 [Hamby, Kelly A.] Univ Maryland, Dept Entomol, 4112 Plant Sci Bldg, College Pk, MD 20742 USA. [Bellamy, David E.] Ocean Spray Cranberries Inc, 1480 State Route 105, Aberdeen, WA 98520 USA. [Chiu, Joanna C.] Univ Calif Davis, Dept Entomol & Nematol, One Shields Ave, Davis, CA 95616 USA. [Lee, Jana C.] USDA ARS, Hort Crops Res Unit, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. [Walton, Vaughn M.; York, Riki M.] Oregon State Univ, Dept Hort, 4105 Ag & Life Sci Bldg, Corvallis, OR 97331 USA. [Wiman, Nik G.] North Willamette Res & Extens Ctr, Dept Hort, Aurora, OR 97002 USA. [Biondi, Antonio] Univ Catania, Dept Agr Food & Environm, Via Santa Sofia 100, I-95123 Catania, Italy. RP Hamby, KA (reprint author), Univ Maryland, Dept Entomol, 4112 Plant Sci Bldg, College Pk, MD 20742 USA. EM kahamby@umd.edu FU USDA SCRI Grant [2010-51181-21167]; USDA CRIS [5358-22000-037-00D]; European Union [318246]; Italian Ministry of Education, University and Research [2010CXXHJE_004, RBSI14I02A] FX We thank Adam Cave, Christina Fieland, and Amanda Lake, USDA-ARS Corvalis, OR, and Daniel Dalton, Oregon State University, for assistance and rearing of D. suzukii used in assays. We thank Nathalie Steinhauer, University of Maryland, for translating Chabert et al. (2013). We thank Dennis Margosan, USDA-ARS Parlier, CA, for his SEM images. Funding was provided by the USDA SCRI Grant 2010-51181-21167, and USDA CRIS 5358-22000-037-00D. Antonio Biondi received funding from the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme FP7/2007-2013/under REA grant agreement 318246, and from the Italian Ministry of Education, University and Research (PRIN project GEISCA, 2010CXXHJE_004, and SIR project ENTOBIONANO, RBSI14I02A). NR 87 TC 4 Z9 4 U1 44 U2 56 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 EI 1612-4766 J9 J PEST SCI JI J. Pest Sci. PD JUL PY 2016 VL 89 IS 3 SI SI BP 605 EP 619 DI 10.1007/s10340-016-0756-5 PG 15 WC Entomology SC Entomology GA DR3LF UT WOS:000379804000002 ER PT J AU Haye, T Girod, P Cuthbertson, AGS Wang, XG Daane, KM Hoelmer, KA Baroffio, C Zhang, JP Desneux, N AF Haye, T. Girod, P. Cuthbertson, A. G. S. Wang, X. G. Daane, K. M. Hoelmer, K. A. Baroffio, C. Zhang, J. P. Desneux, N. TI Current SWD IPM tactics and their practical implementation in fruit crops across different regions around the world SO JOURNAL OF PEST SCIENCE LA English DT Review DE Drosophila suzukii; Biological control; Cultural control; Chemical control ID DROSOPHILA-SUZUKII DIPTERA; SPOTTED WING DROSOPHILA; ASOBARA-JAPONICA HYMENOPTERA; BENEFICIAL ARTHROPODS; HOST ASSOCIATIONS; METHYL-BROMIDE; MELANOGASTER; PARASITOIDS; PEST; INSECTICIDES AB After its arrival in 2008, the Spotted Wing Drosophila (SWD), Drosophila suzukii, has emerged as a harmful invasive insect pest in North America and Europe. This highly polyphagous pest is a major threat to many economically important fruit crops and is also known to develop on a wide variety of natural host plants. In Asia, Europe and North America, different control measures are applied against SWD, such as chemical, biological, and cultural control. Current controls of SWD rely primarily on the application of insecticides, but cultural management tactics such as sanitation and the use of nets provide a good alternative in some crops. Biological control measures, such as conservation of existing natural enemies in invaded areas, introduction of specialized larval parasitoids from Asia for classical biological control and the use of indigenous parasitoids for augmentative control, are currently being investigated and may become an important management tool in the near future for an area-wide control of SWD. C1 [Haye, T.; Girod, P.] CABI, Rue Grillons 1, CH-2800 Delemont, Switzerland. [Cuthbertson, A. G. S.] Fera, York YO41 1LZ, N Yorkshire, England. [Wang, X. G.; Daane, K. M.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Hoelmer, K. A.] ARS, USDA, Beneficial Insects Intro Res Unit, Newark, DE USA. [Baroffio, C.] Agroscope IPS, Res Ctr Conthey VS, Route Eterpys, CH-1964 Conthey, Switzerland. [Haye, T.; Zhang, J. P.] MoA CABI Joint Lab Biosafety, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China. [Desneux, N.] Univ Nice Sophia Antipolis, CNRS, INRA French Natl Inst Agr Res, UMR 1355 7254,Inst Sophia Agrobiotech, F-06903 Sophia Antipolis, France. RP Haye, T (reprint author), CABI, Rue Grillons 1, CH-2800 Delemont, Switzerland.; Haye, T (reprint author), MoA CABI Joint Lab Biosafety, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China. EM t.haye@cabi.org OI GIROD, Pierre/0000-0002-1992-7020 FU USDA APHIS [14-8130-0463]; National Institute of Food and Agriculture, USDA SCRI Initiative [2015-51181-24252]; European Union [613678, 318246]; California Cherry Board FX Funding for research in the US was supported by California Cherry Board, USDA APHIS (Farm Bill, Fund 14-8130-0463) and the National Institute of Food and Agriculture, USDA SCRI Initiative (Agreement No. 2015-51181-24252). USDA is an equal opportunity employer. Research in Europe has received funding from the European Union's Seventh Framework programme for research, technological development and demonstration under grant agreement numbers 613678 (DROPSA) and 318246 (ASCII). NR 106 TC 13 Z9 13 U1 43 U2 49 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 EI 1612-4766 J9 J PEST SCI JI J. Pest Sci. PD JUL PY 2016 VL 89 IS 3 SI SI BP 643 EP 651 DI 10.1007/s10340-016-0737-8 PG 9 WC Entomology SC Entomology GA DR3LF UT WOS:000379804000005 ER PT J AU Wiman, NG Dalton, DT Anfora, G Biondi, A Chiu, JC Daane, KM Gerdeman, B Gottardello, A Hamby, KA Isaacs, R Grassi, A Ioriatti, C Lee, JC Miller, B Stacconi, MVR Shearer, PW Tanigoshi, L Wang, XG Walton, VM AF Wiman, Nik G. Dalton, Daniel T. Anfora, Gianfranco Biondi, Antonio Chiu, Joanna C. Daane, Kent M. Gerdeman, Beverly Gottardello, Angela Hamby, Kelly A. Isaacs, Rufus Grassi, Alberto Ioriatti, Claudio Lee, Jana C. Miller, Betsey Stacconi, M. Valerio Rossi Shearer, Peter W. Tanigoshi, Lynell Wang, Xingeng Walton, Vaughn M. TI Drosophila suzukii population response to environment and management strategies SO JOURNAL OF PEST SCIENCE LA English DT Article DE DD; Temperature; Fecundity; Survival; Population dynamics; Pesticide; Spotted wing Drosophila ID SPOTTED-WING DROSOPHILA; INTEGRATED PEST-MANAGEMENT; CODLING MOTH LEPIDOPTERA; DIPTERA DROSOPHILIDAE; PARASITOIDS ATTACKING; FRUIT PRODUCTION; UNITED-STATES; HYMENOPTERA; DISTRIBUTIONS; INFESTATION AB Drosophila suzukii causes economic damage to berry and stone fruit worldwide. Laboratory-generated datasets were standardized and combined on the basis of degree days (DD), using Gompertz and Cauchy curves for survival and reproduction. Eggs transitioned to larvae at 20.3 DD; larvae to pupae at 118.1 DD; and pupae to adults at 200 DD. All adults are expected to have died at 610 DD. Oviposition initiates at 210 DD and gradually increases to a maximum of 15 eggs per DD at 410 DD and subsequently decreases to zero at 610 DD. These data were used as the basis for a DD cohort-level population model. Laboratory survival under extreme temperatures when DD did not accumulate was described by a Gompertz curve based on calendar days. We determined that the initiation of the reproductive period of late dormant field-collected female D. suzukii ranged from 50 to 800 DD from January 1. This suggests that D. suzukii females can reproduce early in the season and are probably limited by availability of early host plants. Finally, we used the DD population model to examine hypothetical stage-specific mortality effects of IPM practices from insecticides and parasitoids at the field level. We found that adulticides applied during the early season will result in the largest comparative population decrease. It is clear from model outputs that parasitism levels comparable to those found in field studies may have a limited effect on population growth. Novel parasitoid guilds could therefore be improved and would be valuable for IPM of D. suzukii. C1 [Wiman, Nik G.; Dalton, Daniel T.; Miller, Betsey; Walton, Vaughn M.] Oregon State Univ, Dept Hort, 4017 Ag & Life Sci Bldg, Corvallis, OR 97331 USA. [Anfora, Gianfranco; Gottardello, Angela; Grassi, Alberto; Ioriatti, Claudio; Stacconi, M. Valerio Rossi] Fdn Edmund Mach, Res & Innovat Ctr, Via E Mach 1, I-38010 San Michele All Adige, TN, Italy. [Anfora, Gianfranco; Gottardello, Angela; Grassi, Alberto; Ioriatti, Claudio; Stacconi, M. Valerio Rossi] Fdn Edmund Mach, Technol Transfer Ctr, Via E Mach 1, I-38010 San Michele All Adige, TN, Italy. [Biondi, Antonio] Univ Catania, Dept Agr Food & Environm, Via Santa Sofia 100, I-95123 Catania, Italy. [Chiu, Joanna C.] Univ Calif Davis, Dept Entomol & Nematol, Davis, CA 95616 USA. [Daane, Kent M.; Wang, Xingeng] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Gerdeman, Beverly; Tanigoshi, Lynell] Washington State Univ, Dept Entomol, Mt Vernon Northwestern Washington Res & Extens Ct, Mt Vernon, WA 98273 USA. [Hamby, Kelly A.] Univ Maryland, Dept Entomol, 4112 Plant Sci Bldg, College Pk, MD 20742 USA. [Isaacs, Rufus] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [Lee, Jana C.] USDA ARS, Hort Crops Res Unit, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. [Shearer, Peter W.] Oregon State Univ, Mid Columbia Agr Res & Extens Ctr, Hood River, OR 97031 USA. [Walton, Vaughn M.] Oregon State Univ, Dept Hort, 4105C ALS, Corvallis, OR 97331 USA. RP Walton, VM (reprint author), Oregon State Univ, Dept Hort, 4017 Ag & Life Sci Bldg, Corvallis, OR 97331 USA.; Walton, VM (reprint author), Oregon State Univ, Dept Hort, 4105C ALS, Corvallis, OR 97331 USA. EM vaughn.walton@oregonstate.edu OI ANFORA, Gianfranco/0000-0003-2545-1409 FU USDA-NIFA award [2010-51181-21167]; USDA CRIS [5358-22000-037-00D]; Autonomous Province of Trento (Italy); Grandi Progetti, Project LExEM (Laboratory of excellence for epidemiology and modeling); California Cherry Board; USDA APHIS [14-8130-0463]; European Union [318246]; Italian Ministry of Education, University and Research [2010CXXHJE_004] FX Funding for research in the U.S.A. was provided from the USDA-NIFA award # 2010-51181-21167 and USDA CRIS 5358-22000-037-00D. In Italy, this research was partially funded by the Autonomous Province of Trento (Italy) and research funds from the Grandi Progetti, Project LExEM (Laboratory of excellence for epidemiology and modeling, http://www.lexem.eu). We thank the Oregon State Blueberry Commission, the Washington State Blueberry Commission, the Washington State Red Raspberry Commission, the Washington State Strawberry Commission, the Washington State Commission on Pesticide Registration, Project GREEEN, and the Michigan State Horticultural Society. We thank the California Cherry Board and USDA APHIS (Farm Bill, fund 14-8130-0463) for partial funding of this study. Antonio Biondi received funding from the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme FP7/2007-2013/under REA grant agreement 318246 and from the Italian Ministry of Education, University and Research (PRIN project GEISCA, 2010CXXHJE_004). Molly Albrecht provided assistance with D. suzukii dissections. NR 67 TC 3 Z9 3 U1 26 U2 31 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 EI 1612-4766 J9 J PEST SCI JI J. Pest Sci. PD JUL PY 2016 VL 89 IS 3 SI SI BP 653 EP 665 DI 10.1007/s10340-016-0757-4 PG 13 WC Entomology SC Entomology GA DR3LF UT WOS:000379804000006 ER PT J AU Lee, JC Dalton, DT Swoboda-Bhattarai, KA Bruck, DJ Burrack, HJ Strik, BC Woltz, JM Walton, VM AF Lee, Jana C. Dalton, Daniel T. Swoboda-Bhattarai, Katharine A. Bruck, Denny J. Burrack, Hannah J. Strik, Bernadine C. Woltz, J. Megan Walton, Vaughn M. TI Characterization and manipulation of fruit susceptibility to Drosophila suzukii SO JOURNAL OF PEST SCIENCE LA English DT Article DE Brix; Firmness; Fruit preference; pH; Spotted wing drosophila ID DIPTERA DROSOPHILIDAE; BLUEBERRY; OVIPOSITION; CULTIVARS; QUALITY; CHERRY; HOST AB Drosophila suzukii (Matsumura) is an economic pest of small fruits and cherries that attacks intact ripening fruits. Host susceptibility may be influenced by characteristics such as flesh firmness, penetration force of the skin, total soluble solids (TSS, also known as A degrees Brix), and pH. Improved knowledge of factors affecting fruit susceptibility is needed for developing thresholds and risk prediction models for IPM. A combination of laboratory and field studies was conducted to develop prediction and potential management tools. First, a direct bioassay was used to calculate the probability of oviposition in a given fruit based on various characteristics as determined across laboratory and field trials in Oregon and North Carolina, US. When multiple characteristics were evaluated simultaneously, oviposition probability consistently increased as penetration force decreased and pH increased. Oviposition probability sometimes increased as TSS increased. Second, raspberries and blueberries in unsprayed fields had substantially lower infestation in ripening fruit compared to ripe fruit. There was no or minimal infestation in green fruit. Third, given that skin penetration force influences oviposition, practices used to improve fruit quality were examined in laboratory no-choice cages for potential reduction of oviposition. Blueberry fruit sprayed with calcium silicate in the field had greater penetration force and firmness and reduced number of eggs laid by D. suzukii compared to untreated fruit. Other calcium-based treatments increased Ca content and firmness of fruit relative to untreated fruit. Timing of insecticide spray for D. suzukii might be delayed until fruit become susceptible. C1 [Lee, Jana C.; Bruck, Denny J.; Woltz, J. Megan] ARS, USDA, Hort Crops Res Unit, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. [Dalton, Daniel T.; Strik, Bernadine C.; Woltz, J. Megan; Walton, Vaughn M.] Oregon State Univ, Dept Hort, 4017 Ag & Life Sci Bldg, Corvallis, OR 97331 USA. [Swoboda-Bhattarai, Katharine A.; Burrack, Hannah J.] North Carolina State Univ, Dept Entomol, Campus Box 7630, Raleigh, NC 27695 USA. [Bruck, Denny J.] DuPont Pioneer, 7300 NW 62nd Ave, Johnston, IA 50131 USA. RP Lee, JC (reprint author), ARS, USDA, Hort Crops Res Unit, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. EM jana.lee@ars.usda.gov FU Northwest Center for Small Fruit Research; USDA SCRI [2010-51181-21167]; USDA CRIS [5358-22000-037-00D] FX We are grateful to Austin Cuenca, Christina Fieland, Jesse Mindolovich, Danielle Selleck, and Jeff Wong for assistance with the studies. We thank Dave Bryla and Chad Finn for use of the small fruit plots, and two anonymous growers. Funding was provided by the Northwest Center for Small Fruit Research, USDA SCRI Grant 2010-51181-21167, and USDA CRIS 5358-22000-037-00D. NR 22 TC 9 Z9 9 U1 16 U2 23 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 EI 1612-4766 J9 J PEST SCI JI J. Pest Sci. PD JUL PY 2016 VL 89 IS 3 SI SI BP 771 EP 780 DI 10.1007/s10340-015-0692-9 PG 10 WC Entomology SC Entomology GA DR3LF UT WOS:000379804000016 ER PT J AU Lee, JC Dalton, DT Swoboda-Bhattarai, KA Bruck, DJ Burrack, HJ Strik, BC Woltz, JM Walton, VM AF Lee, Jana C. Dalton, Daniel T. Swoboda-Bhattarai, Katharine A. Bruck, Denny J. Burrack, Hannah J. Strik, Bernadine C. Woltz, J. Megan Walton, Vaughn M. TI Characterization and manipulation of fruit susceptibility to Drosophila suzukii (vol 89, pg 771, 2016) SO JOURNAL OF PEST SCIENCE LA English DT Correction C1 [Lee, Jana C.; Bruck, Denny J.; Woltz, J. Megan] ARS, USDA, Hort Crops Res Unit, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. [Dalton, Daniel T.; Strik, Bernadine C.; Woltz, J. Megan; Walton, Vaughn M.] Oregon State Univ, Dept Hort, 4017 Ag & Life Sci Bldg, Corvallis, OR 97331 USA. [Swoboda-Bhattarai, Katharine A.; Burrack, Hannah J.] North Carolina State Univ, Dept Entomol, Campus Box 7630, Raleigh, NC 27695 USA. [Bruck, Denny J.] DuPont Pioneer, 7300 NW 62nd Ave, Johnston, IA 50131 USA. RP Lee, JC (reprint author), ARS, USDA, Hort Crops Res Unit, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. EM jana.lee@ars.usda.gov NR 1 TC 0 Z9 0 U1 5 U2 5 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 EI 1612-4766 J9 J PEST SCI JI J. Pest Sci. PD JUL PY 2016 VL 89 IS 3 SI SI BP 781 EP 782 DI 10.1007/s10340-015-0718-3 PG 2 WC Entomology SC Entomology GA DR3LF UT WOS:000379804000017 ER PT J AU Tochen, S Walton, VM Lee, JC AF Tochen, Samantha Walton, Vaughn M. Lee, Jana C. TI Impact of floral feeding on adult Drosophila suzukii survival and nutrient status SO JOURNAL OF PEST SCIENCE LA English DT Article DE Glycogen; Nectar; Lipid; Sugar ID SPOTTED-WING DROSOPHILA; DIPTERA DROSOPHILIDAE; PSEUDACTEON-TRICUSPIS; NUTRITIONAL STATE; PARASITOID WASP; SUGAR ANALYSIS; PHORID FLY; FRUIT-FLY; LIFE-SPAN; REPRODUCTION AB Drosophila suzukii, spotted wing drosophila, is a serious pest of small fruits and cherries in many regions of the world. While host usage has been well studied at the ovipositional and larval feeding stages, little is known about the feeding ecology and nutrient requirements of adults. This study addressed the impact of feeding on the survival and nutrient reserves of adult D. suzukii in laboratory assays. First, access to cherry blossoms increased survival rates of both adult males and females compared to water only. This suggests that these early spring blossoms may provide a food source for D. suzukii in fields that may be devoid of other food sources. Second, D. suzukii reared on a standard laboratory diet as larvae emerged as adults with minimal glycogen and sugar levels. Adults with continued access to a carbohydrate-protein diet showed rapidly elevated carbohydrate reserves, and adults with continued access to only water showed a decline in total sugars. Third, females with access to cherry or blueberry blossoms showed elevated carbohydrate reserves when compared to those with access to water only. These results illustrate the importance of adult feeding in enhancing survival and carbohydrate reserves among D. suzukii. C1 [Tochen, Samantha; Walton, Vaughn M.] Oregon State Univ, Dept Hort, 4017 Ag & Life Sci Bldg, Corvallis, OR 97331 USA. [Lee, Jana C.] ARS, USDA, Hort Crops Res Unit, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. RP Tochen, S (reprint author), Oregon State Univ, Dept Hort, 4017 Ag & Life Sci Bldg, Corvallis, OR 97331 USA. EM samantha.tochen@oregonstate.edu FU USDA SCRI [2010-51181-21167]; USDA CRIS [5358-22000-037-00D, 2072-22000-040-00D] FX We are grateful for Adam Cave for rearing D. suzukii, and Danielle Selleck and Jeff Wong for assistance with assays. We thank Anita Azarenko, Eric Pond, and Jay Pscheidt for access to fresh blueberry and cherry blossoms. Funding was provided by the USDA SCRI Grant 2010-51181-21167, and USDA CRIS 5358-22000-037-00D and 2072-22000-040-00D. NR 54 TC 0 Z9 0 U1 16 U2 21 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 EI 1612-4766 J9 J PEST SCI JI J. Pest Sci. PD JUL PY 2016 VL 89 IS 3 SI SI BP 793 EP 802 DI 10.1007/s10340-016-0762-7 PG 10 WC Entomology SC Entomology GA DR3LF UT WOS:000379804000019 ER PT J AU Daane, KM Wang, XG Biondi, A Miller, B Miller, JC Riedl, H Shearer, PW Guerrieri, E Giorgini, M Buffington, M van Achterberg, K Song, Y Kang, T Yi, H Jung, C Lee, DW Chung, BK Hoelmer, KA Walton, VM AF Daane, Kent M. Wang, Xin-Geng Biondi, Antonio Miller, Betsey Miller, Jeffrey C. Riedl, Helmut Shearer, Peter W. Guerrieri, Emilio Giorgini, Massimo Buffington, Matthew van Achterberg, Kees Song, Yoohan Kang, Taegun Yi, Hoonbok Jung, Chuleui Lee, Dong Woon Chung, Bu-Keun Hoelmer, Kim A. Walton, Vaughn M. TI First exploration of parasitoids of Drosophila suzukii in South Korea as potential classical biological agents SO JOURNAL OF PEST SCIENCE LA English DT Article DE Biological control; Invasive species; Parasitoid; Spotted wing drosophila ID SPOTTED-WING DROSOPHILA; ASOBARA-JAPONICA HYMENOPTERA; DIPTERA DROSOPHILIDAE; UNITED-STATES; LARVAL PARASITOIDS; PRODUCTION REGIONS; FRUIT PRODUCTION; INVASIVE PEST; HOST; BRACONIDAE AB The invasive spotted wing drosophila, Drosophila suzukii Matsumura (Dipt.: Drosophilidae), a native of East Asia, has widely established in North America and Europe, where it is a serious pest of small and stone fruit crops. The lack of effective indigenous parasitoids of D. suzukii in the recently colonized regions prompted the first foreign exploration for co-evolved parasitoids in South Korea during 2013 and 2014. We collected the larval parasitoids Asobara japonica Belokobylskij, A. leveri (Nixon) and A. brevicauda Guerrieri & van Achterberg (Hym.: Braconidae), Ganaspis brasiliensis (Ihering), Leptopilina japonica japonica NovkoviA double dagger & Kimura and L. j. formosana NovkoviA double dagger & Kimura (Hym.: Figitidae); and the pupal parasitoids Pachycrepoideus vindemiae (Rondani) (Hym.: Pteromalidae) and Trichopria drosophilae Perkins (Hym.: Diapriidae). From UC Berkeley quarantine records, percentage parasitism ranged from 0 to 17.1 % and varied by geography, season, and collection methods. Asobara japonica was the most common parasitoid species. Higher numbers of parasitoids were reared from field-picked fruit as opposed to traps baited with uninfested fruit. Quarantine bioassays confirmed that A. japonica, G. brasiliensis, L. j. japonica, P. vindemiae, and T. drosophilae developed from D. suzukii. Female individuals of the endoparasitoid, A. japonica, were larger when reared on the larger D. suzukii larvae compared with those reared on the smaller larvae of D. melanogaster Meigen. Larger parasitoid size was associated with longer developmental time. Several of the South Korean parasitoid species have the potential for use in classical biological control and may contribute to the suppression of D. suzukii in the newly invaded regions. C1 [Daane, Kent M.; Wang, Xin-Geng; Biondi, Antonio] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Biondi, Antonio] Univ Catania, Dept Agr Food & Environm, Catania, Italy. [Miller, Betsey; Miller, Jeffrey C.; Walton, Vaughn M.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Riedl, Helmut; Shearer, Peter W.] Oregon State Univ, Mid Columbia Extens & Expt Stn, Dept Hort, Hood River, OR USA. [Guerrieri, Emilio; Giorgini, Massimo] CNR, Inst Sustainable Plant Protect, Portici, Italy. [Buffington, Matthew] ARS, Systemat Entomol Lab, USDA, Washington, DC USA. [van Achterberg, Kees] Natl Museum Nat Hist, Dept Entomol, Leiden, Netherlands. [Song, Yoohan; Kang, Taegun] Gyeongsang Univ, Jinju, South Korea. [Yi, Hoonbok] Seoul Womens Univ, Seoul, South Korea. [Jung, Chuleui] Andong Natl Univ, Dept Plant Med, Andong, South Korea. [Lee, Dong Woon] Kyungpook Natl Univ, Dept Appl Biol, Daegu, South Korea. [Chung, Bu-Keun] Gyeongnam Agr Res & Extens Serv, Jinju, South Korea. [Hoelmer, Kim A.] ARS, Beneficial Insects Intro Res Unit, USDA, Newark, DE USA. RP Daane, KM (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. EM kdaane@ucanr.edu FU European Union [ASCII-PIRSES 318246]; Italian Ministry of Education, University and Research [2010CXXHJE_004]; USDA-NIFA award [2010-51181-21167]; USDA APHIS [14-8130-0463]; California Cherry Board; UE FP7 project ASCII [PIRSES-GA-2012-318246] FX We thank Riki York and Kyoo Park (Oregon State University), Hyun Jung Kim and Jongwoo Nam (Seoul Women's University), Uk-Jin Park and Rameswor Maharjan (Andong National University), Oh-Gyeong Kwon (Kyungpook National University), Deuk So Choi (Department of Plant Quarantine, Anyangsi), and Iksoo Kim (Chonnam National University, Gwangju) for assistance with collections in South Korea and Hongyin Chen and Chenxi Liu (Chinese Academy of Agricultural Science), Zon Qi Chen and Yang Wang (Yunnan Academy of Agricultural Science) for collections in China; John Hutchins and Brandy Chavez (University of California, Berkeley) for assistance with insect rearing in the quarantine; and Ken Bloem (Biological Control Coordinator, USDA, APHIS, PPQ) for program design. Antonio Biondi was supported through the People Programme of the European Union's Seventh Framework Programme FP7/2007-2013 Project ASCII-PIRSES 318246 and from the Italian Ministry of Education, University and Research (PRIN project GEISCA, 2010CXXHJE_004). Funding for research was supported in the US by the USDA-NIFA award # 2010-51181-21167, the USDA APHIS (Farm bill, fund 14-8130-0463), and the California Cherry Board, and in Italy by the UE FP7/2007-2013 project ASCII under Grant agreement PIRSES-GA-2012-318246. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer. NR 48 TC 7 Z9 7 U1 17 U2 21 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 EI 1612-4766 J9 J PEST SCI JI J. Pest Sci. PD JUL PY 2016 VL 89 IS 3 SI SI BP 823 EP 835 DI 10.1007/s10340-016-0740-0 PG 13 WC Entomology SC Entomology GA DR3LF UT WOS:000379804000022 ER PT J AU Ren, H Liu, H Wang, J Yuan, LL Cui, XD Zhang, QM Fu, L Chen, HF Zhong, WC Yang, KM Guo, QF AF Ren, Hai Liu, Hong Wang, Jun Yuan, Lianlian Cui, Xiaodong Zhang, Qianmei Fu, Lin Chen, Hongfeng Zhong, Wenchao Yang, Keming Guo, Qinfeng TI The use of grafted seedlings increases the success of conservation translocations of Manglietia longipedunculata (Magnoliaceae), a Critically Endangered tree SO ORYX LA English DT Article DE China; conservation introduction; ecological restoration; Manglietia longipedunculata; plant conservation technique; point endemic species; threatened species ID ASSISTED COLONIZATION; CLIMATE-CHANGE; PLANT; REINTRODUCTION; CHINA; POPULATION; GENETICS; RESTORATION; HISTORY; DEBATE AB The distribution of the Critically Endangered tree Manglietia longipedunculata, of which there are only 11 known wild individuals, is restricted to the Nankunshan Nature Reserve in South China. The species is threatened with extinction because of its small number of individuals and the impediments to its reproduction (a combination of protogyny, a short period of stigma receptivity, and a lack of efficient pollinators). To reduce the risk of extinction we conducted two conservation translocation trials: one to augment the sole extant population, and the other 202 km north of the current range. The latter trial was a conservation introduction in which the goals were to increase the population and to buffer against the effects of climate change. We used emerged and grafted seedlings as translocation materials. We compared the survival, growth, and eco-physiological properties of emerged and grafted seedlings at the two sites. The survival rate and growth were higher for grafted seedlings than for emerged seedlings at both sites. Eco-physiological data indicated that grafted seedlings at both sites were as efficient or more so in light and water usage than wild individuals, whereas emerged seedlings were less efficient. Grafted seedlings attained the flowering stage sooner than emerged seedlings. Our study suggests that grafting can facilitate the augmentation and establishment of new populations of M. longipedunculata and perhaps of new populations of other threatened species facing reproductive difficulties and climate change. C1 [Ren, Hai; Wang, Jun; Yuan, Lianlian; Zhang, Qianmei; Fu, Lin; Chen, Hongfeng; Yang, Keming] Chinese Acad Sci, South China Bot Garden, Key Lab Vegetat Restorat & Management Degraded Ec, Guangzhou 510650, Guangdong, Peoples R China. [Liu, Hong] Florida Int Univ, Int Ctr Trop Bot, Dept Earth & Environm, Miami, FL 33199 USA. [Liu, Hong] Guangxi Univ, Coll Forestry, Nanning, Peoples R China. [Cui, Xiaodong; Zhong, Wenchao] Adm Nankunshan Nat Reserve, Longmeng, Peoples R China. [Guo, Qinfeng] US Forest Serv, Southern Res Stn, USDA, Asheville, NC USA. RP Ren, H (reprint author), Chinese Acad Sci, South China Bot Garden, Key Lab Vegetat Restorat & Management Degraded Ec, Guangzhou 510650, Guangdong, Peoples R China. EM renhai@scbg.ac.cn FU National Natural Science Foundation of China [31170493, 31360146]; Guangdong Science and Technology Programme [2013B060400016, 2010B060200039]; Guangxi Science and Technology Bureau [12217-04] FX This research was supported by the National Natural Science Foundation of China (31170493, 31360146), Guangdong Science and Technology Programme (2013B060400016, 2010B060200039), and the Guangxi Science and Technology Bureau (grant no. 12217-04). We are grateful to Prof. Qingwen Zeng, Dr Chen Xiuzi and Mr Zhixin Tang at the Administration of Tianxin Provincial Natural Reserve for the field investigation. We also thank Prof. Bruce Jaffee and Ms Elizabeth Platt for editing the English, and constructive comments. NR 45 TC 0 Z9 0 U1 10 U2 15 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0030-6053 EI 1365-3008 J9 ORYX JI Oryx PD JUL PY 2016 VL 50 IS 3 BP 437 EP 445 DI 10.1017/S0030605315000423 PG 9 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DR4OW UT WOS:000379882000013 ER PT J AU Claeson, SM Coffin, B AF Claeson, S. M. Coffin, B. TI Physical and Biological Responses to an Alternative Removal Strategy of a Moderate-sized Dam in Washington, USA SO RIVER RESEARCH AND APPLICATIONS LA English DT Article DE Hemlock Dam; restoration; temperature; turbidity; sediment; macroinvertebrate; dam removal ID LOW-HEAD DAM; STREAM; MACROINVERTEBRATE; ASSEMBLAGES; RIVER AB Dam removal is an increasingly practised river restoration technique, and ecological responses vary with watershed, dam and reservoir properties, and removal strategies. Moderate-sized dams, like Hemlock Dam (7.9m tall and 56m wide), are large enough that removal effects could be significant, but small enough that mitigation may be possible through a modified dam removal strategy. The removal of Hemlock Dam in Washington State, USA, was designed to limit channel erosion and improve fish passage and habitat by excavating stored fine sediment and reconstructing a channel in the former 6-ha reservoir. Prior to dam removal, summer daily water temperatures downstream from the dam increased and remained warm long into the night. Afterwards, a more natural diel temperature regime was restored, although daily maximum temperatures remained high. A short-lived turbidity pulse occurred soon after re-watering of the channel, but was otherwise similar to background levels. Substrate shifted from sand to gravel-cobble in the former reservoir and from boulder to gravel-cobble downstream of the dam. Initially, macroinvertebrate assemblage richness and abundance was low in the project area, but within 2years, post-removal reaches upstream and downstream of the dam had diverse and abundant communities. The excavation of stored sediment and channel restoration as part of the dam removal strategy restored river continuity and improved benthic habitat while minimizing downstream sedimentation. This study provides a comparison of ecological effects with other dam removal strategies and can inform expectations of response time and magnitude. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. C1 [Claeson, S. M.] USDA Forest Serv, Pacific Northwest Res Stn, Wenatchee, WA 98807 USA. [Coffin, B.] USDA Forest Serv, Mt Adams Ranger Dist, Trout Lake, WA USA. RP Claeson, SM (reprint author), USDA Forest Serv, Pacific Northwest Res Stn, Wenatchee, WA 98807 USA. EM sclaeson@fs.fed.us NR 28 TC 0 Z9 0 U1 34 U2 46 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1535-1459 EI 1535-1467 J9 RIVER RES APPL JI River Res. Appl. PD JUL PY 2016 VL 32 IS 6 BP 1143 EP 1152 DI 10.1002/rra.2935 PG 10 WC Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA DR5OS UT WOS:000379952900001 ER PT J AU Hough-Snee, N Kasprak, A Rossi, RK Bouwes, N Roper, BB Wheaton, JM AF Hough-Snee, N. Kasprak, A. Rossi, R. K. Bouwes, N. Roper, B. B. Wheaton, J. M. TI Hydrogeomorphic and Biotic Drivers of Instream Wood Differ Across Sub-basins of the Columbia River Basin, USA SO RIVER RESEARCH AND APPLICATIONS LA English DT Article DE large woody debris; ecogeomorphology; wood transport; riparian vegetation; stream assessment; aquatic habitat; Columbia Habitat Monitoring Program; riparian ecology ID COLORADO FRONT RANGE; RIPARIAN VEGETATION; WESTERN WASHINGTON; CHANNEL MORPHOLOGY; DEBRIS RECRUITMENT; MOUNTAIN STREAMS; WATERSHED-SCALE; COASTAL MAINE; OLD-GROWTH; HABITAT AB Instream wood promotes habitat heterogeneity through its influence on flow hydraulics and channel geomorphology. Within the Columbia River Basin, USA, wood is vital for the creation and maintenance of habitat for threatened salmonids. However, our understanding of the relative roles of the climatic, geomorphic, and ecological processes that source wood to streams is limited, making it difficult to identify baseline predictions of instream wood and create targets for stream restoration. Here, we investigate how instream wood frequency and volume differ between seven sub-basins of the interior Columbia River Basin and what processes shape these differences within these sub-basins. We collected data on wood volume and frequency, discharge and stream power, and riparian and watershed forest structure for use in modelling wood volume and frequency. Using random forest models, we found that mean annual precipitation, riparian tree cover, and the individual watershed were the most important predictors of wood volume and frequency. Within sub-basins, we used linear models, finding that some basins had unique predictors of wood. Discharge, watershed area, or precipitation often combined with forest cover, riparian conifer, and/or large tree cover in models of instream large wood volume and frequency. In many sub-basins, models showed at least one hydrologic variable, indicative of transport competence and one ecological variable, indicative of the reach or upstream watershed's capability to grow measurable instream wood. We conclude that basin-specific models yield important insights into the hydrologic and ecological processes that influence wood loads, creating tractable hypotheses for building predictive models of instream wood. Copyright (c) 2015 John Wiley & Sons, Ltd. C1 [Hough-Snee, N.; Kasprak, A.; Rossi, R. K.; Bouwes, N.; Roper, B. B.; Wheaton, J. M.] Utah State Univ, Dept Watershed Sci, 5210 Old Main Hill, Logan, UT 84322 USA. [Hough-Snee, N.; Wheaton, J. M.] Utah State Univ, Ctr Ecol, Logan, UT 84322 USA. [Bouwes, N.] Eco Log Res Inc, Providence, RI USA. [Roper, B. B.] USDA Forest Serv, Forest Sci Lab, Logan, UT USA. RP Hough-Snee, N (reprint author), Utah State Univ, Dept Watershed Sci, 5210 Old Main Hill, Logan, UT 84322 USA. EM nate@natehough-snee.org OI Hough-Snee, Nate/0000-0003-4581-0931; Wheaton, Joseph/0000-0002-8361-8150 FU Bonneville Power Administration (BPA) [2003-017]; ELR (USU) [100652]; STAR by the US Environmental Protection Agency (EPA) [91768201-0] FX Support was provided by grants from the Bonneville Power Administration to Eco Logical Research, Inc. (BPA Project Number: 2003-017) and subsequent grants from ELR to Utah State University (USU Award ID: 100652). N.H-S. was supported in part by STAR Fellowship Assistance Agreement No. 91768201-0 awarded by the US Environmental Protection Agency (EPA). This research has not been formally reviewed by the EPA, NOAA, USFS, nor BPA, and the views expressed here are solely those of the authors. The EPA, NOAA, USFS, and BPA do not endorse any products or commercial services mentioned in this publication. We thank landowners for access and CHaMP crews and partner organizations for data collection. We thank Reid Camp, Dan Hamill, James Gore, Martha Jensen, Wally MacFarlane, and Peter McHugh for helpful feedback on manuscript drafts and Brandon Dickinson for facilitating writing. Simon Dixon provided a particularly thorough and helpful review of this work for which we are very grateful. The authors declare no conflict of interests. N.H-S., N. B., J. M. W., and B. B. R. conceptualized the project; N. H-S. performed the analyses; and N. H-S. and R. K. R. created the figures and tables. All authors wrote the manuscript. N. B., B. B. R., and J. M. W. made equal contributions and are listed alphabetically as authors. NR 69 TC 0 Z9 0 U1 12 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1535-1459 EI 1535-1467 J9 RIVER RES APPL JI River Res. Appl. PD JUL PY 2016 VL 32 IS 6 BP 1302 EP 1315 DI 10.1002/rra.2968 PG 14 WC Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA DR5OS UT WOS:000379952900015 ER PT J AU Sheng, Y Davidson, A Fuglie, K Zhang, DD AF Sheng, Yu Davidson, Alistair Fuglie, Keith Zhang, Dandan TI Input Substitution, Productivity Performance and Farm Size SO AUSTRALIAN JOURNAL OF AGRICULTURAL AND RESOURCE ECONOMICS LA English DT Article DE elasticity of technical substitution; income effect; total factor productivity ID ECONOMIC-GROWTH; ELASTICITIES; EFFICIENCY; AGRICULTURE; VARIABLES AB This paper develops a theoretical model to examine the relationship between the input elasticity of (technical) substitution and both farm total factor productivity and size. In the presence of ongoing technical change and its factor bias, the income effect' arising from farms' cost minimising behaviour enables them to increase productivity by saving inputs or, through the dual equivalent, enlarging farm size. As such, farms with higher elasticities of substitution tend to grow larger and become more productive, which provides a new mechanism through which farm heterogeneity in productivity growth can be examined. Empirical evidence from Australian broadacre agriculture supports this theory and points to important policy implications. C1 [Sheng, Yu; Davidson, Alistair] Australian Bur Agr & Resource Econ & Sci, Canberra, ACT, Australia. [Fuglie, Keith] Econ Res Serv, USDA, Washington, DC USA. [Zhang, Dandan] Peking Univ, Natl Sch Dev, Beijing, Peoples R China. RP Sheng, Y (reprint author), Australian Bur Agr & Resource Econ & Sci, Canberra, ACT, Australia. EM yu.sheng@agriculture.gov.au NR 35 TC 0 Z9 0 U1 11 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-985X EI 1467-8489 J9 AUST J AGR RESOUR EC JI Aust. J. Agr. Resour. Econ. PD JUL PY 2016 VL 60 IS 3 BP 327 EP 347 DI 10.1111/1467-8489.12136 PG 21 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA DR5HO UT WOS:000379934300004 ER PT J AU Small, GE Finlay, JC McKay, RML Rozmarynowycz, MJ Brovold, S Bullerjahn, GS Spokas, K Sterner, RW AF Small, Gaston E. Finlay, Jacques C. McKay, R. M. L. Rozmarynowycz, Mark J. Brovold, Sandra Bullerjahn, George S. Spokas, Kurt Sterner, Robert W. TI Large differences in potential denitrification and sediment microbial communities across the Laurentian great lakes SO BIOGEOCHEMISTRY LA English DT Article DE Denitrification; Great Lakes; Nitrate; Nitrogen; Lake Erie; Lake Superior; Microbial community ID COASTAL MARINE ECOSYSTEMS; NITROGEN REMOVAL; FRESH-WATER; BACTERIA; DIVERSITY; SEQUENCES; SUPERIOR; QUALITY; EUTROPHICATION; GREENGENES AB Large lakes can efficiently remove reactive nitrogen (N) through denitrification, but nitrate levels in some large oligotrophic lakes are increasing, indicating that denitrification in these lakes is not capable of removing excess N. To better understand how lake trophic status and sediment redox conditions affect the capacity of the microbial community to remove excess N, we measured potential denitrification rates at 86 different stations across Lakes Superior, Huron, Erie, and Ontario. We also relate sediment microbial communities to potential denitrification rates and sediment characteristics for a subset of these sites. In eutrophic/mesotrophic Lake Erie, characterized by sediment with minimal oxygen penetration and relatively high sediment carbon (C) and N, potential denitrification rates were relatively high and increased by 2-3 orders of magnitude in response to additional nitrate and organic C. In contrast, in oligotrophic Lakes Superior and Ontario, and mesotrophic Lake Huron, where oxygen can penetrate several cm into sediment, potential denitrification rates were generally low and did not respond to additional nitrate and organic carbon. Sediment microbial communities showed a similar pattern across this gradient, correlated with potential denitrification rates, sediment %C, and bottom-water nitrate concentrations. This observed relationships between sediment redox conditions, potential denitrification rates, and microbial diversity suggest that sediment microbial communities in these and other oligotrophic large lakes may already be operating at or near their maximum denitrification rates. Unlike mesotrophic Lake Erie, microbial communities in oligotrophic lake sediments may lack the ability to mitigate increases in N loading through denitrification. C1 [Small, Gaston E.] Univ St Thomas, Dept Biol, 2115 Summit Ave, St Paul, MN 55105 USA. [Finlay, Jacques C.] Univ Minnesota Twin Cities, Dept Ecol Evolut & Behav, 1987 Upper Buford, St Paul, MN 55108 USA. [McKay, R. M. L.; Rozmarynowycz, Mark J.; Bullerjahn, George S.] Bowling Green State Univ, Dept Biol Sci, Bowling Green, OH 43403 USA. [Brovold, Sandra; Sterner, Robert W.] Univ Minnesota Duluth, Large Lakes Observ, 2205 E 5th St, Duluth, MN 55812 USA. [Spokas, Kurt] ARS, USDA, St Paul, MN 55108 USA. [Spokas, Kurt] Univ Minnesota Twin Cities, Dept Soil Water & Climate, 1991 Upper Buford Circle, St Paul, MN 55108 USA. RP Small, GE (reprint author), Univ St Thomas, Dept Biol, 2115 Summit Ave, St Paul, MN 55105 USA. EM gaston.small@stthomas.edu RI Spokas, Kurt/F-4839-2016; OI Spokas, Kurt/0000-0002-5049-5959; Small, Gaston/0000-0002-9018-7555 FU National Science Foundation [OCE-0927512, OCE-0927277]; Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231]; Community Sequencing Project [723] FX We thank the crew of the R/V Blue Heron for their support during this study, and the U.S. Coast Guard, Canadian Coast Guard, and Environment Canada for providing additional sampling opportunities. This material is based upon work supported by the National Science Foundation under Grants OCE-0927512 and OCE-0927277. The work conducted by the U.S. Department of Energy Joint Genome Institute was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231 and Community Sequencing Project 723. NR 52 TC 0 Z9 0 U1 17 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-2563 EI 1573-515X J9 BIOGEOCHEMISTRY JI Biogeochemistry PD JUL PY 2016 VL 128 IS 3 BP 353 EP 368 DI 10.1007/s10533-016-0212-x PG 16 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA DQ5BT UT WOS:000379220100006 ER PT J AU Bahaa-el-Din, L Sollmann, R Hunter, LTB Slotow, R Macdonald, DW Henschel, P AF Bahaa-el-Din, Laila Sollmann, Rahel Hunter, Luke T. B. Slotow, Rob Macdonald, David W. Henschel, Philipp TI Effects of human land-use on Africa's only forest-dependent fetid: The African golden cat Caracal aurata SO BIOLOGICAL CONSERVATION LA English DT Article DE Bushmeat; Camera trap; Density; Gabon; Logging; Spatial capture-recapture ID MALAYSIAN BORNEO; CAMERA-TRAP; DENSITY; CARNIVORES; MAMMALS; LEOPARD; ABUNDANCE; SELECTION; RESERVES; BELIZE AB Africa's equatorial forests are threatened by widespread deforestation and bushmeat hunting, with both threats spreading into formerly remote areas due to rapid human population growth and large-scale expansions of commercial resource extraction such as logging and mining, as well as forest clearing for agriculture. Many globally threatened species are endemic to these forests, but the potential effects of these threats are not well understood. Using the case of the forest-dependent African golden cat, we assess the potential effects of disturbance including logging and hunting on population density. We applied spatially-explicit capture-recapture models to camera trap data to estimate density across a human land-use gradient at five sites in central Gabon. We found density was highest at a pristine, undisturbed site (16.23 [+/- 5.84 SE] individuals per 100 km(2)) and lowest at a village site with moderate levels of mostly subsistence bushmeat hunting (3.8[+/- 2.23 SE] individuals per 100 km(2)). Logging concessions can support important densities of the species (10.18 [+/- 3.54 SE] and 12.84 [+/- 4.25 SE] individuals per 100 km(2)), with the higher estimate of the two for the concession certified by the Forest Stewardship Council (FSC) versus the non-certified concession. While protected intact forests are the main strongholds for golden cats, well-managed logging concessions may also play an important role in the conservation of golden cats and other threatened species. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Bahaa-el-Din, Laila; Slotow, Rob] Univ Kwazulu Natal, Sch Life Sci, Westville Campus,Private Bag X54001, ZA-4000 Durban, South Africa. [Bahaa-el-Din, Laila; Hunter, Luke T. B.; Henschel, Philipp] Panthera, 8 West 40th St,18th Fl, New York, NY 10018 USA. [Bahaa-el-Din, Laila; Macdonald, David W.] Univ Oxford, Dept Zool, Wildlife Conservat Res Unit WiIdCRU, Recanati Kaplan Ctr, Tubney House,Abingdon Rd, Tubney OX13 5QL, England. [Sollmann, Rahel] Univ Calif Davis, Dept Wildlife Fish & Conservat Biol, 1088 Acad Surge,One Shields Ave, Davis, CA 95616 USA. [Sollmann, Rahel] US Forest Serv, USDA, Pacific Southwest Res Stn, 1731 Res Pk Dr, Davis, CA 95618 USA. RP Bahaa-el-Din, L (reprint author), Univ Kwazulu Natal, Sch Life Sci, Westville Campus,Private Bag X54001, ZA-4000 Durban, South Africa. EM lailab.sp@gmail.com FU Panthera; Wildlife Conservation Research Unit (WildCRU); University of KwaZuluNatal (UKZN); International Society for Endangered Cats (ISEC) Canada; El Tigre Fund FX Funding for this study was received from Panthera, the Wildlife Conservation Research Unit (WildCRU) and the University of KwaZuluNatal (UKZN). Members of all of these institutions are co-authors of this study, and were involved in helping to design the study and prepare the manuscript for publication. Additional funding was received from the International Society for Endangered Cats (ISEC) Canada, and the El Tigre Fund, neither of which were involved with study design, implementation or manuscript preparation. NR 47 TC 0 Z9 0 U1 29 U2 45 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0006-3207 EI 1873-2917 J9 BIOL CONSERV JI Biol. Conserv. PD JUL PY 2016 VL 199 BP 1 EP 9 DI 10.1016/j.biocon.2016.04.013 PG 9 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DQ9WI UT WOS:000379559600001 ER PT J AU Gaskin, JF Pokorny, ML Mangold, JM AF Gaskin, John F. Pokorny, Monica L. Mangold, Jane M. TI An unusual case of seed dispersal in an invasive aquatic; yellow flag iris (Iris pseudacorus) SO BIOLOGICAL INVASIONS LA English DT Article DE Invasive aquatic plant; Iris pseudacorus; Reproduction; Yellow flag iris; AFLP ID MULTILOCUS GENOTYPE DATA; POPULATION-STRUCTURE; GENETIC-VARIATION; EICHHORNIA-CRASSIPES; ALTERNANTHERA-PHILOXEROIDES; EXTREME VARIATION; PLANT; SOFTWARE; CHINA; REPRODUCTION AB Invasive aquatic plants typically reproduce vegetatively, but there have been conflicting hypotheses of invasive aquatic yellow flag iris dispersing primarily by seed versus rhizome fragmentation. We performed genetic analysis of 20 aquatic yellow flag iris populations across the Pacific Northwest, USA, with leaf tissue taken from plants between 2 and 5 m apart. We found 167 unique genotypes in 171 plants, and we never found genetically identical plants from different populations. We found that 99.1 % of seed is viable. Our results support that this obligately outcrossing invasive disperses almost entirely by seed, not rhizome fragmentation. We found no significant relationship between genetic and geographic distance across the Pacific Northwest, suggesting recent long distance dispersal and/or multiple founding events. Bayesian analysis shows the presence of two major genotypic clusters within our collections which also suggests more than one distinct genetic source for the invasion. These processes have led to genetically distinct populations that can be geographically close. Our findings are unusual for an aquatic invasive, and inform yellow flag iris managers of two things: (1) to limit dispersal, development of mature seed in the field should be prevented; and (2) if classical biological control is proposed, an agent guild that limits seed production would be effective for managing most dispersal. C1 [Gaskin, John F.] ARS, USDA, 1500 North Cent Ave, Sidney, MT 59270 USA. [Pokorny, Monica L.; Mangold, Jane M.] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. RP Gaskin, JF (reprint author), ARS, USDA, 1500 North Cent Ave, Sidney, MT 59270 USA. EM john.gaskin@ars.usda.gov FU Bureau of Land Management (Montana, South and North Dakota) Billings Office FX This research was made possible in part by funding from the Bureau of Land Management (Montana, South and North Dakota) Billings Office. Thanks to K. Mann and J. Lassey for generating AFLP data. NR 50 TC 0 Z9 0 U1 6 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD JUL PY 2016 VL 18 IS 7 BP 2067 EP 2075 DI 10.1007/s10530-016-1151-0 PG 9 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DP9LA UT WOS:000378816500019 ER PT J AU Pourmokhtarian, A Driscoll, CT Campbell, JL Hayhoe, K Stoner, AMK AF Pourmokhtarian, Afshin Driscoll, Charles T. Campbell, John L. Hayhoe, Katharine Stoner, Anne M. K. TI The effects of climate downscaling technique and observational data set on modeled ecological responses SO ECOLOGICAL APPLICATIONS LA English DT Article DE climate change; ecological modeling; forested watershed; Hubbard Brook Experimental Forest (HBEF); New Hampshire; USA; hydrology; LTER; net primary productivity; statistical downscaling; stream nitrate; uncertainty analysis; variability ID NORTHERN HARDWOOD FOREST; NORTHEASTERN UNITED-STATES; WATER-RESOURCES; CO2 ENRICHMENT; SURFACE-WATER; RIVER-BASIN; LAND-USE; PHOTOSYNTHESIS; DEPOSITION; HYDROLOGY AB Assessments of future climate change impacts on ecosystems typically rely on multiple climate model projections, but often utilize only one downscaling approach trained on one set of observations. Here, we explore the extent to which modeled biogeochemical responses to changing climate are affected by the selection of the climate downscaling method and training observations used at the montane landscape of the Hubbard Brook Experimental Forest, New Hampshire, USA. We evaluated three downscaling methods: the delta method (or the change factor method), monthly quantile mapping (Bias Correction-Spatial Disaggregation, or BCSD), and daily quantile regression (Asynchronous Regional Regression Model, or ARRM). Additionally, we trained outputs from four atmosphere-ocean general circulation models (AOGCMs) (CCSM3, HadCM3, PCM, and GFDL-CM2.1) driven by higher (A1fi) and lower (B1) future emissions scenarios on two sets of observations (1/8 degrees resolution grid vs. individual weather station) to generate the high-resolution climate input for the forest biogeochemical model PnET-BGC (eight ensembles of six runs). The choice of downscaling approach and spatial resolution of the observations used to train the downscaling model impacted modeled soil moisture and streamflow, which in turn affected forest growth, net N mineralization, net soil nitrification, and stream chemistry. All three downscaling methods were highly sensitive to the observations used, resulting in projections that were significantly different between station-based and grid-based observations. The choice of downscaling method also slightly affected the results, however not as much as the choice of observations. Using spatially smoothed gridded observations and/or methods that do not resolve sub-monthly shifts in the distribution of temperature and/or precipitation can produce biased results in model applications run at greater temporal and/or spatial resolutions. These results underscore the importance of carefully considering field observations used for training, as well as the downscaling method used to generate climate change projections, for smaller-scale modeling studies. Different sources of variability including selection of AOGCM, emissions scenario, downscaling technique, and data used for training downscaling models, result in a wide range of projected forest ecosystem responses to future climate change. C1 [Pourmokhtarian, Afshin; Driscoll, Charles T.] Syracuse Univ, Dept Civil & Environm Engn, Syracuse, NY 13244 USA. [Campbell, John L.] US Forest Serv, Northern Res Stn, Durham, NH 03824 USA. [Hayhoe, Katharine; Stoner, Anne M. K.] Texas Tech Univ, Climate Sci Ctr, Lubbock, TX 79409 USA. RP Pourmokhtarian, A (reprint author), Syracuse Univ, Dept Civil & Environm Engn, Syracuse, NY 13244 USA. EM afshin.pourmokhtarian@gmail.com OI Driscoll, Charles/0000-0003-2692-2890 FU Environmental Protection Agency; USDA Northeastern States Research Cooperative; National Science Foundation (NSF) through the Long-Tenn Ecological Research (LTER) program; NSF FX The authors would like to thank the two anonymous reviewers for providing helpful comments that improved this manuscript. Funding for this study was provided by the Environmental Protection Agency through the STAR program, the USDA Northeastern States Research Cooperative, and the National Science Foundation (NSF) through the Long-Tenn Ecological Research (LTER) program. This manuscript is a contribution of the Hubbard Brook Ecosystem Study. Hubbard Brook is part of the LTER network, which is supported by the NSF. The Hubbard Brook Experimental Forest is operated and maintained by the USDA Forest Service, Northern Research Station, Newtown Square, Pennsylvania, USA. NR 59 TC 1 Z9 1 U1 8 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD JUL PY 2016 VL 26 IS 5 BP 1321 EP 1337 DI 10.1890/15-0745 PG 17 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DR0NJ UT WOS:000379603900004 PM 27755746 ER PT J AU Irisarri, JGN Derner, JD Porensky, LM Augustine, DJ Reeves, JL Mueller, KE AF Irisarri, J. Gonzalo N. Derner, Justin D. Porensky, Lauren M. Augustine, David J. Reeves, Justin L. Mueller, Kevin E. TI Grazing intensity differentially regulates ANPP response to precipitation in North American semiarid grasslands SO ECOLOGICAL APPLICATIONS LA English DT Article DE northern mixed-grass prairie; precipitation marginal response; precipitation-use efficiency; rain-use efficiency; rangeland ecosystems; shortgrass steppe ID RAIN-USE EFFICIENCY; GREAT-PLAINS; PRIMARY PRODUCTIVITY; COMMUNITY STRUCTURE; ECONOMIC RESPONSES; SHORTGRASS STEPPE; FUNCTIONAL TYPES; UNITED-STATES; STOCKING RATE; C-4 GRASSES AB Grazing intensity elicits changes in the composition of plant functional groups in both shortgrass steppe (SGS) and northern mixed-grass prairie (NMP) in North America. How these grazing intensity-induced changes control aboveground net primary production (ANPP) responses to precipitation remains a central open question, especially in light of predicted climate changes. Here, we evaluated effects of four levels (none, light, moderate, and heavy) of long-term (>30 yr) grazing intensity in SGS and NMP on: (1) ANPP; (2) precipitation-use efficiency (PUE, ANPP:precipitation); and (3) precipitation marginal response (PMR; slope of a linear regression model between ANPP and precipitation). We advance prior work by examining: (1) the consequences of a range of grazing intensities (more grazed vs. ungrazed); and (2) how grazing-induced changes in ANPP and PUE are related both to shifts in functional group composition and physiological responses within each functional group. Spring (April-June) precipitation, the primary determinant of ANPP, was only 12% higher in NMP than in SGS, yet ANPP and PUE were 25% higher. Doubling grazing intensity in SGS and nearly doubling it in NMP reduced ANPP and PUE by only 24% and 33%, respectively. Increased grazing intensity reduced C-3 graminoid biomass and increased C-4 grass biomass in both grasslands. Functional group shifts affected PUE through biomass reductions, as PUE was positively associated with the relative abundance of C-3 species and negatively with C-4 species across both grasslands. At the community level, PMR was similar between grasslands and unaffected by grazing intensity. However, PMR of C-3 graminoids in SGS was eightfold higher in the ungrazed treatment than under any grazed level. In NMP, PMR of C-3 graminoids was only reduced under heavy grazing intensity. Knowing the ecological consequences of grazing intensity provides valuable information for mitigation and adaptation strategies in response to predicted climate change. For example, moderate grazing (the recommended rate) in SGS would sequester the same amount of aboveground carbon as light grazing because ANPP was nearly the same. In contrast, reductions in grazing intensity in NMP from moderate to light intensity would increase the amount of aboveground carbon sequestrated by 25% because of increased ANPP. C1 [Irisarri, J. Gonzalo N.] Univ Buenos Aires, Fac Agron, CONICET, IFEVA, Ave San Martin 4453,C1417DSE, Buenos Aires, DF, Argentina. [Derner, Justin D.; Porensky, Lauren M.; Augustine, David J.; Reeves, Justin L.; Mueller, Kevin E.] ARS, USDA, Rangeland Resources Res Unit, Cheyenne, WY 82009 USA. [Derner, Justin D.; Porensky, Lauren M.; Augustine, David J.; Reeves, Justin L.; Mueller, Kevin E.] ARS, USDA, Rangeland Resources Res Unit, Ft Collins, CO 80526 USA. RP Derner, JD (reprint author), ARS, USDA, Rangeland Resources Res Unit, Cheyenne, WY 82009 USA.; Derner, JD (reprint author), ARS, USDA, Rangeland Resources Res Unit, Ft Collins, CO 80526 USA. EM Justin.Derner@ars.usda.gov FU USDA-Agricultural Research Service FX Crow Valley Livestock Cooperative, Inc., provided the yearlings at the Central Plains Experimental Range, and the University of Wyoming and 8A Hay and Cattle Company provided the yearlings at the High Plains Grasslands Research Station. We appreciate the field and infrastructure contributions of technicians Mary Ashby, Matt Mortenson and Jeff Thomas, as well as the field contributions by numerous summer students. Funding provided by the USDA-Agricultural Research Service. NR 58 TC 1 Z9 1 U1 18 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD JUL PY 2016 VL 26 IS 5 BP 1370 EP 1380 DI 10.1890/15-1332 PG 11 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DR0NJ UT WOS:000379603900007 PM 27755747 ER PT J AU Bhomia, RK MacKenzie, RA Murdiyarso, D Sasmito, SD Purbopuspito, J AF Bhomia, R. K. MacKenzie, R. A. Murdiyarso, D. Sasmito, S. D. Purbopuspito, J. TI Impacts of land use on Indian mangrove forest carbon stocks: Implications for conservation and management SO ECOLOGICAL APPLICATIONS LA English DT Article DE Bhitarkanika Conservation Area; India; blue carbon; carbon stocks; coastal ecosystems; climate change mitigation; Indian mangroves ID CLIMATE-CHANGE MITIGATION; BIOMASS; ALLOMETRY; ECOSYSTEM; DYNAMICS; STORAGE; COAST AB Globally, mangrove forests represents only 0.7% of world's tropical forested area but are highly threatened due to susceptibility to climate change, sea level rise, and increasing pressures from human population growth in coastal regions. Our study was carried out in the Bhitarkanika Conservation Area (BCA), the second-largest mangrove area in eastern India. We assessed total ecosystem carbon (C) stocks at four land use types representing varying degree of disturbances. Ranked in order of increasing impacts, these sites included dense mangrove forests, scrub mangroves, restored/planted mangroves, and abandoned aquaculture ponds. These impacts include both natural and/or anthropogenic disturbances causing stress, degradation, and destruction of mangroves. Mean vegetation C stocks (including both above-and belowground pools; mean +/- standard error) in aquaculture, planted, scrub, and dense mangroves were 0, 7 +/- 4, 65 +/- 11 and 100 +/- 11 Mg C/ha, respectively. Average soil C pools for aquaculture, planted, scrub, and dense mangroves were 61 +/- 8, 92 +/- 20, 177 +/- 14, and 134 +/- 17 Mg C/ha, respectively. Mangrove soils constituted largest fraction of total ecosystem C stocks at all sampled sites (aquaculture [100%], planted [90%], scrub [72%], and dense mangrove [57%]). Within BCA, the four studied land use types covered an area of similar to 167 km(2) and the total ecosystem C stocks were 0.07 Tg C for aquaculture (similar to 12 km(2)), 0.25 Tg C for planted/restored mangrove (similar to 24 km(2)), 2.29 teragrams (Tg) Tg C for scrub (similar to 93 km(2)), and 0.89 Tg C for dense mangroves (similar to 38 km(2)). Although BCA is protected under Indian wildlife protection and conservation laws, similar to 150 000 people inhabit this area and are directly or indirectly dependent on mangrove resources for sustenance. Estimates of C stocks of Bhitarkanika mangroves and recognition of their role as a C repository could provide an additional reason to support conservation and restoration of Bhitarkanika mangroves. Harvesting or destructive exploitation of mangroves by local communities for economic gains can potentially be minimized by enabling these communities to avail themselves of carbon offset/conservation payments under approved climate change mitigation strategies and actions. C1 [Bhomia, R. K.] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Bhomia, R. K.; Murdiyarso, D.; Sasmito, S. D.] Ctr Int Forestry Res CIFOR, Jalan CIFOR, Bogor 16115, Indonesia. [MacKenzie, R. A.] US Forest Serv, USDA, Pacific Southwest Res Stn, Inst Pacific Isl Forestry, Hilo, HI 96720 USA. [Murdiyarso, D.] Bogor Agr Univ, Dept Geophys & Meteorol, Darmaga Campus, Bogor 16152, Indonesia. [Purbopuspito, J.] Sam Ratulangi Univ, Soil Sci Dept, Kampus Kleak Bahu, Manado 95115, Indonesia. RP Bhomia, RK (reprint author), Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA.; Bhomia, RK (reprint author), Ctr Int Forestry Res CIFOR, Jalan CIFOR, Bogor 16115, Indonesia. EM rbhomia@ufl.edu RI Sasmito, Sigit D./E-8715-2013; Purbopuspito, Joko/P-1165-2015 OI Sasmito, Sigit D./0000-0001-5864-8596; Purbopuspito, Joko/0000-0002-4631-596X FU United States Agency for International Development (USAID) FX This study was carried out under the Sustainable Wetlands Adaptation and Mitigation Program (SWAMP), a collaborative effort by the Center for International Forestry Research (CIFOR), Oregon State University, and the United States Forest Service (USFS), with support from the United States Agency for International Development (USAID). We would like to acknowledge the help and support provided by P. Das from SFTRRD and A. K. Sahoo from OUAT. We thank the Principal Chief Conservator of Forests, Govt. of Odisha, and Divisional Forest Officer, Rajnagar for granting permission to conduct sampling in Bhitarkanika area. We would also like to thank C. S. Kar and S. D. Pradhan for interactive discussions related to Bhitarkanika ecosystem which helped to understand the unique history and ecology of the area. We are grateful to the team of hardworking and committed participants who enabled data collection in the field. We also acknowledge the analytical lab at Central Rice Research Institute, Cuttack, for help in the carbon analysis of soil samples. We are thankful to two anonymous reviewers and one colleague for their useful comments and suggestions which helped significantly to improve the quality of this article. NR 67 TC 0 Z9 0 U1 20 U2 38 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD JUL PY 2016 VL 26 IS 5 BP 1396 EP 1408 DI 10.1890/15-2143 PG 13 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DR0NJ UT WOS:000379603900009 PM 27755754 ER PT J AU Lovett, GM Weiss, M Liebhold, AM Holmes, TP Leung, B Lambert, KF Orwig, DA Campbell, FT Rosenthal, J McCullough, DG Wildova, R Ayres, MP Canham, CD Foster, DR LaDeau, SL Weldy, T AF Lovett, Gary M. Weiss, Marissa Liebhold, Andrew M. Holmes, Thomas P. Leung, Brian Lambert, Kathy Fallon Orwig, David A. Campbell, Faith T. Rosenthal, Jonathan McCullough, Deborah G. Wildova, Radka Ayres, Matthew P. Canham, Charles D. Foster, David R. LaDeau, Shannon L. Weldy, Troy TI Nonnative forest insects and pathogens in the United States: Impacts and policy options SO ECOLOGICAL APPLICATIONS LA English DT Article DE disease; forest; insect; invasive; pathogen; policy ID EMERALD ASH BORER; BEECH BARK DISEASE; AGRILUS-PLANIPENNIS FAIRMAIRE; WOOLLY ADELGID INFESTATION; EASTERN NORTH-AMERICA; LONG-TERM CHANGES; BIOLOGICAL INVASIONS; SPECIES COMPOSITION; COMMUNITY STRUCTURE; CATSKILL MOUNTAINS AB We review and synthesize information on invasions of nonnative forest insects and diseases in the United States, including their ecological and economic impacts, pathways of arrival, distribution within the United States, and policy options for reducing future invasions. Nonnative insects have accumulated in United States forests at a rate of similar to 2.5 per yr over the last 150 yr. Currently the two major pathways of introduction are importation of live plants and wood packing material such as pallets and crates. Introduced insects and diseases occur in forests and cities throughout the United States, and the problem is particularly severe in the Northeast and Upper Midwest. Nonnative forest pests are the only disturbance agent that has effectively eliminated entire tree species or genera from United States forests within decades. The resulting shift in forest structure and species composition alters ecosystem functions such as productivity, nutrient cycling, and wildlife habitat. In urban and suburban areas, loss of trees from streets, yards, and parks affects aesthetics, property values, shading, stormwater runoff, and human health. The economic damage from nonnative pests is not yet fully known, but is likely in the billions of dollars per year, with the majority of this economic burden borne by municipalities and residential property owners. Current policies for preventing introductions are having positive effects but are insufficient to reduce the influx of pests in the face of burgeoning global trade. Options are available to strengthen the defenses against pest arrival and establishment, including measures taken in the exporting country prior to shipment, measures to ensure clean shipments of plants and wood products, inspections at ports of entry, and post-entry measures such as quarantines, surveillance, and eradication programs. Improved data collection procedures for inspections, greater data accessibility, and better reporting would support better evaluation of policy effectiveness. Lack of additional action places the nation, local municipalities, and property owners at high risk of further damaging and costly invasions. Adopting stronger policies to reduce establishments of new forest insects and diseases would shift the major costs of control to the source and alleviate the economic burden now borne by homeowners and municipalities. C1 [Lovett, Gary M.; Canham, Charles D.; LaDeau, Shannon L.] Cary Inst Ecosyst Studies, Box AB, Millbrook, NY 12545 USA. [Weiss, Marissa; Lambert, Kathy Fallon] Harvard Univ, Harvard Forest, Sci Policy Exchange, Petersham, MA 01366 USA. [Weiss, Marissa; Lambert, Kathy Fallon; Orwig, David A.; Foster, David R.] Harvard Univ, Harvard Forest, Petersham, MA 01366 USA. [Liebhold, Andrew M.] US Forest Serv, USDA, Northern Res Stn, Morgantown, WV 26505 USA. [Holmes, Thomas P.] US Forest Serv, USDA, Southern Res Stn, Res Triangle Pk, NC 27701 USA. [Leung, Brian] McGill Univ, Dept Biol, Montreal, PQ H3A 1B1, Canada. [Campbell, Faith T.] Ctr Invas Species Prevent, Bethesda, MD 20814 USA. [Rosenthal, Jonathan; Wildova, Radka] Inst Ecol Res, Kingston, NY 12401 USA. [McCullough, Deborah G.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [McCullough, Deborah G.] Michigan State Univ, Dept Forestry, E Lansing, MI 48824 USA. [Ayres, Matthew P.] Dartmouth Coll, Dept Biol, Hanover, NH 03755 USA. [Weldy, Troy] Nature Conservancy, New York State Chapter, Albany, NY 12205 USA. RP Lovett, GM (reprint author), Cary Inst Ecosyst Studies, Box AB, Millbrook, NY 12545 USA. EM lovettg@caryinstitute.org FU Doris Duke Charitable Foundation; F.M. Kirby Foundation; Northeastern States Research Cooperative of the USDA Forest Service; U.S. National Science Foundation's Long Term Ecological Research program [DEB 11-14804, DEB 12-37491] FX We arc grateful to the Doris Duke Charitable Foundation, the F.M. Kirby Foundation, and Northeastern States Research Cooperative of the USDA Forest Service for support of the project that led to this paper. Additional support was provided by the U.S. National Science Foundation's Long Term Ecological Research program through grants DEB 11-14804 (Hubbard Brook site) and DEB 12-37491 (Harvard Forest site). The conclusions and opinions in this paper are those of the authors and not the NSRC. the Forest Service, or the USDA. We thank Stas Burgiel, Arm Gibbs, and Rebecca Turner for advice on policy issues, Kurt Gottschalk for a helpful review of the manuscript, and the U.S. Forest Service's Forest Health Technology Enterprise Team for calculations of forest area at risk. NR 111 TC 3 Z9 4 U1 47 U2 75 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD JUL PY 2016 VL 26 IS 5 BP 1437 EP 1455 DI 10.1890/15-1176 PG 19 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DR0NJ UT WOS:000379603900012 PM 27755760 ER PT J AU Ganzlin, PW Gundale, MJ Becknell, RE Cleveland, CC AF Ganzlin, Peter W. Gundale, Michael J. Becknell, Rachel E. Cleveland, Cory C. TI Forest restoration treatments have subtle long-term effects on soil C and N cycling in mixed conifer forests SO ECOLOGICAL APPLICATIONS LA English DT Article DE biogeochemistry; fire suppression; forest restoration; nutrient cycling; Pinus ponderosa; prescribed fire; thinning ID PONDEROSA PINE FORESTS; ALTERS NITROGEN TRANSFORMATIONS; LOW ELEVATION FORESTS; TERRESTRIAL ECOSYSTEMS; WESTERN MONTANA; ECOLOGICAL RESTORATION; LITTER DECOMPOSITION; PRESCRIBED FIRE; CELLULOSE DECOMPOSITION; INLAND NORTHWEST AB Decades of fire suppression following extensive timber harvesting have left much of the forest in the intermountain western United States exceedingly dense, and forest restoration techniques (i.e., thinning and prescribed fire) are increasingly being used in an attempt to mitigate the effects of severe wildfire, to enhance tree growth and regeneration, and to stimulate soil nutrient cycling. While many of the short-term effects of forest restoration have been established, the long-term effects on soil biogeochemical and ecosystem processes are largely unknown. We assessed the effects of commonly used forest restoration treatments (thinning, burning, and thinning + burning) on nutrient cycling and other ecosystem processes 11 yr after restoration treatments were implemented in a ponderosa pine (Pinus ponderosa var. scopulorum)/Douglas fir (Pseudotsuga menziesii var. glauca) forest at the Lubrecht Fire and Fire Surrogates Study (FFS) site in western Montana, USA. Despite short-term (< 3 yr) increases in soil inorganic nitrogen (N) pools and N cycling rates following prescribed fire, long-term soil N pools and N mineralization rates showed only subtle differences from untreated control plots. Similarly, despite a persistent positive correlation between fuels consumed in prescribed burns and several metrics of N cycling, variability in inorganic N pools decreased significantly since treatments were implemented, indicating a decline in N spatial heterogeneity through time. However, rates of net nitrification remain significantly higher in a thin + burn treatment relative to other treatments. Short-term declines in forest floor carbon (C) pools have persisted in the thin + burn treatment, but there were no significant long-term differences among treatments in extractable soil phosphorus (P). Finally, despite some short-term differences, long-term foliar nutrient concentrations, litter decomposition rates, and rates of free-living N fixation in the experimental plots were not different from control plots, suggesting nutrient cycles and ecosystem processes in temperate coniferous forests are resilient to disturbance following long periods of fire suppression. Overall, this study provides forest managers and policy-makers valuable information showing that the effects of these commonly used restoration prescriptions on soil nutrient cycling are ephemeral and that use of repeated treatments (i. e., frequent fire) will be necessary to ensure continued restoration success. C1 [Ganzlin, Peter W.; Cleveland, Cory C.] Univ Montana, Coll Forestry & Conservat, Dept Ecosyst & Conservat Sci, Missoula, MT 59812 USA. [Gundale, Michael J.] Swedish Univ Agr Sci, Dept Forest Ecol & Management, S-90183 Umea, Sweden. [Becknell, Rachel E.] Univ Missouri, Dept Biol, 8001 Nat Bridge Rd, St Louis, MO 63121 USA. [Ganzlin, Peter W.] ARS, USDA, Soil & Water Management Res Unit, 1991 Upper Buford Circle, St Paul, MN 55108 USA. RP Cleveland, CC (reprint author), Univ Montana, Coll Forestry & Conservat, Dept Ecosyst & Conservat Sci, Missoula, MT 59812 USA. EM cory.cleveland@umontana.edu RI Gundale, Michael /O-1265-2013 OI Gundale, Michael /0000-0003-2447-609X FU National Institute of Food and Agriculture, U.S. Department of Agriculture, McIntire-Stennis [229018] FX This material is based upon work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, McIntire-Stennis under Accession No. 229018. We thank M. Young and the UM Environmental Biogeochemistry Laboratory for assisting with soil elemental analyses, C. Fiedler for his guidance at the Lubrecht FFS site, and S. Castle, M. Nasto, B. Sullivan, and two anonymous reviewers for providing valuable comments on the manuscript. We are also grateful for the help of several undergraduate lab assistants from the University of Montana who assisted with this research in the field and laboratory, including A. Ginter, E. Prag, and N. Boote. NR 105 TC 1 Z9 1 U1 28 U2 43 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD JUL PY 2016 VL 26 IS 5 BP 1503 EP 1516 DI 10.1890/15-1100 PG 14 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DR0NJ UT WOS:000379603900016 PM 27755759 ER PT J AU Smith, MC Lake, EC Wheeler, GS AF Smith, Melissa C. Lake, Ellen C. Wheeler, Gregory S. TI Oviposition choice and larval performance of Neomusotima conspurcatalis on leaflet types of the invasive fern, Lygodium microphyllum SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article DE weed control; biological control; herbivory; volatile leaf extracts; feeding trials; oviposition trials; Lygodiaceae; Lepidoptera; Crambidae ID BIOLOGICAL-CONTROL AGENT; WORLD CLIMBING FERN; LEPIDOPTERA CRAMBIDAE; HOST-RANGE; FLORIDA EVERGLADES; LYGODIACEAE; PREFERENCE; WEED; SELECTION; SCHIZAEACEAE AB Lygodium microphyllum (Cavanilles) R Brown (Lygodiaceae), Old World climbing fern, invades wildlands in central and southern Florida, USA, and causes considerable habitat disturbance. Efforts to develop an effective biological control strategy have focused on several folivorous insects and a leaf-galling mite from southeast Asia and Australia. Neomusotima conspurcatalis Warren (Lepidoptera: Crambidae) is a foliage-feeding moth that specializes on Lygodium species. First released in 2008, N. conspurcatalis successfully established in Florida and is dispersing throughout the L. microphyllum-invaded range. We investigated whether N. conspurcatalis display any preference (oviposition or larval feeding) between two leaflet morphologies, i.e., fertile spore-producing vs. sterile leaflets. Furthermore, we examined whether oviposition and no-choice diets on either fern leaflet type conferred any possible selective advantages (e.g., weight, emergence success) to the offspring. Finally, we analyzed leaf nitrogen and carbon content and leaf volatiles to gauge differences between the leaflet types. In choice and no-choice scenarios, larvae consume approximately the same amount of leaf area and display no preference between fertile and sterile leaflets. However, females deposited significantly more eggs on fertile than on sterile leaflets. Oviposition choice had no effect on larval development times, pupal weights, or successful eclosure. Nitrogen and carbon content did not differ between fertile and sterile samples, but sterile leaflets contain more 1-octen-3-ol, which may act as an herbivory deterrent. The propagule attack imposed by N. conspurcatalis feeding on fertile leaflets is unlikely to provide adequate control, but could be beneficial as part of a suite of biological control agents and other control methods. C1 [Smith, Melissa C.; Lake, Ellen C.; Wheeler, Gregory S.] USDA ARS, Invas Plant Res Lab, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. RP Smith, MC (reprint author), USDA ARS, Invas Plant Res Lab, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. EM melissa.smith@ars.usda.gov FU Florida Fish and Wildlife Conservation Commission; Comprehensive Everglades Restoration Plan; USDA-ARS; US Army Corps of Engineers; South Florida Water Management District FX We would like to acknowledge Gloria Witkus, Alexander Chapman, and Cristina Martinez for their assistance in gathering larval feeding and oviposition data. We thank Evan Broggi for his assistance in collecting volatile emission samples. Field sites were provided by the Florida Department of Transportation, Florida Power and Light, South Florida Water Management Area, The Nature Conservancy, and the National Park Service. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. Funding for this project was generously provided through the Florida Fish and Wildlife Conservation Commission and the Comprehensive Everglades Restoration Plan, a cooperative agreement between USDA-ARS, the US Army Corps of Engineers, and the South Florida Water Management District. NR 46 TC 0 Z9 0 U1 10 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0013-8703 EI 1570-7458 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD JUL PY 2016 VL 160 IS 1 BP 11 EP 17 DI 10.1111/eea.12450 PG 7 WC Entomology SC Entomology GA DR0PA UT WOS:000379608200002 ER PT J AU Metslaid, S Stanturf, JA Hordo, M Korjus, H Laarmann, D Kiviste, A AF Metslaid, Sandra Stanturf, John A. Hordo, Maris Korjus, Henn Laarmann, Diana Kiviste, Andres TI Growth responses of Scots pine to climatic factors on reclaimed oil shale mined land SO ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH LA English DT Article DE Reclamation; Post-mining area; Afforestation; Tree-ring; Scots pine; Forest management ID BASAL AREA INCREMENT; SYLVESTRIS L.; RADIAL GROWTH; LATITUDINAL GRADIENT; QUARRY DETRITUS; DIAMETER GROWTH; FINLAND; ESTONIA; DROUGHT; STANDS AB Afforestation on reclaimed mining areas has high ecological and economic importance. However, ecosystems established on post-mining substrate can become vulnerable due to climate variability. We used tree-ring data and dendrochronological techniques to study the relationship between climate variables and annual growth of Scots pine (Pinus sylvestris L.) growing on reclaimed open cast oil shale mining areas in Northeast Estonia. Chronologies for trees of different age classes (50, 40, 30) were developed. Pearson's correlation analysis between radial growth indices and monthly climate variables revealed that precipitation in June-July and higher mean temperatures in spring season enhanced radial growth of pine plantations, while higher than average temperatures in summer months inhibited wood production. Sensitivity of radial increment to climatic factors on post-mining soils was not homogenous among the studied populations. Older trees growing on more developed soils were more sensitive to precipitation deficit in summer, while growth indices of two other stand groups (young and middle-aged) were highly correlated to temperature. High mean temperatures in August were negatively related to annual wood production in all trees, while trees in the youngest stands benefited from warmer temperatures in January. As a response to thinning, mean annual basal area increment increased up to 50 %. By managing tree competition in the closed-canopy stands, through the thinning activities, tree sensitivity and response to climate could be manipulated. C1 [Metslaid, Sandra; Hordo, Maris; Korjus, Henn; Laarmann, Diana; Kiviste, Andres] Estonian Univ Life Sci, Inst Forestry & Rural Engn, Dept Forest Management, Kreutzwaldi 5, EE-51014 Tartu, Estonia. [Stanturf, John A.] US Forest Serv, Ctr Forest Disturbance Sci, USDA, 320 Green St, Athens, GA 30602 USA. RP Metslaid, S (reprint author), Estonian Univ Life Sci, Inst Forestry & Rural Engn, Dept Forest Management, Kreutzwaldi 5, EE-51014 Tartu, Estonia. EM Sandra.Metslaid@emu.ee RI Metslaid, Sandra/E-1330-2017; OI Korjus, Henn/0000-0001-8522-7869 FU Estonian Ministry of Education and Research [IUT21-4]; SOO project [3.2.0801.11-0012] FX The study was conducted at the Estonian University of Life Sciences. The study was supported by the Institutional Research Funding IUT21-4 of the Estonian Ministry of Education and Research, and SOO project (3.2.0801.11-0012) and the Environmental Investment Center supported data collection from the network of Estonian research plots. Estonian Weather Service at Estonian Environmental Agency provided climate data. We would also like to thank Kalmer Sokman for provided information and reviewers for the valuable comments, which helped to improve manuscript considerably. NR 82 TC 1 Z9 1 U1 2 U2 4 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0944-1344 EI 1614-7499 J9 ENVIRON SCI POLLUT R JI Environ. Sci. Pollut. Res. PD JUL PY 2016 VL 23 IS 14 BP 13637 EP 13652 DI 10.1007/s11356-015-5647-4 PG 16 WC Environmental Sciences SC Environmental Sciences & Ecology GA DQ9TZ UT WOS:000379553500007 PM 26573311 ER PT J AU Eclarinal, JD Zhu, SY Baker, MS Piyarathna, DB Coarfa, C Fiorotto, ML Waterland, RA AF Eclarinal, Jesse D. Zhu, Shaoyu Baker, Maria S. Piyarathna, Danthasinghe B. Coarfa, Cristian Fiorotto, Marta L. Waterland, Robert A. TI Maternal exercise during pregnancy promotes physical activity in adult offspring SO FASEB JOURNAL LA English DT Article DE developmental programming; activity-stat; metabolic imprinting ID ENERGY-EXPENDITURE; VOLUNTARY EXERCISE; LOCOMOTOR-ACTIVITY; FEMALE MICE; OBESITY; LIFE; RAT; HEALTH; AGE; DETERMINANTS AB Previous rodent studies have shown that maternal voluntary exercise during pregnancy leads to metabolic changes in adult offspring. We set out to test whether maternal voluntary exercise during pregnancy also induces persistent changes in voluntary physical activity in the offspring. Adult C57BL/6J female mice were randomly assigned to be caged with an unlocked (U) or locked (L) running wheel before and during pregnancy. Maternal running behavior was monitored during pregnancy, and body weight, body composition, food intake, energy expenditure, total cage activity, and running wheel activity were measured in the offspring at various ages. U offspring were slightly heavier at birth, but no group differences in body weight or composition were observed at later ages (when mice were caged without access to running wheels). Consistent with our hypothesis, U offspring were more physically active as adults. This effect was observed earlier in female offspring (at sexual maturation). Remarkably, at 300 d of age, U females achieved greater fat loss in response to a 3-wk voluntary exercise program. Our findings show for the first time that maternal physical activity during pregnancy affects the offspring's lifelong propensity for physical activity and may have important implications for combating the worldwide epidemic of physical inactivity and obesity. C1 [Eclarinal, Jesse D.; Zhu, Shaoyu; Baker, Maria S.; Fiorotto, Marta L.; Waterland, Robert A.] USDA ARS, Childrens Nutr Res Ctr, Houston, TX USA. [Eclarinal, Jesse D.; Zhu, Shaoyu; Baker, Maria S.; Fiorotto, Marta L.; Waterland, Robert A.] Baylor Coll Med, Dept Pediat, Houston, TX USA. [Piyarathna, Danthasinghe B.; Coarfa, Cristian] Baylor Coll Med, Dept Mol & Cell Biol, Houston, TX USA. [Waterland, Robert A.] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX USA. RP Waterland, RA (reprint author), USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, Dept Pediat, 1100 Bates St,Suite 5080, Houston, TX 77030 USA.; Waterland, RA (reprint author), USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, Dept Mol & Human Genet, 1100 Bates St,Suite 5080, Houston, TX 77030 USA. EM waterland@bcm.edu FU U.S. Department of Agriculture (USDA) Grants [CRIS 6250-51000-055, CRIS 3092-5-001-059]; U.S. National Institutes of Health (NIH) Institute of Arthritis and Musculoskeletal and Skin Diseases Grant [AR46308]; USDA/Agricultural Research Service (ARS) FX The authors thank Mr. Firoz Vohra [U.S. Department of Agriculture (USDA) Children's National Research Center (CNRC), Baylor College of Medicine] for expert assistance with the CLAMS studies, and Mr. Adam Gillum (USDA CNRC) for assistance in creating the figures. This work was funded by U.S. Department of Agriculture (USDA) Grants CRIS 6250-51000-055 and CRIS 3092-5-001-059 (to R.A.W.) and U.S. National Institutes of Health (NIH) Institute of Arthritis and Musculoskeletal and Skin Diseases Grant AR46308 (to M.L.F.). Measurements of body composition, energy expenditure, and food intake were conducted in the Mouse Metabolic Research Unit (USDA CNRC), which is supported by funds from the USDA/Agricultural Research Service (ARS) (www.bcm.edu/cnrc/mmru). The authors declare no conflicts of interest. NR 42 TC 1 Z9 1 U1 8 U2 8 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD JUL PY 2016 VL 30 IS 7 BP 2541 EP 2548 DI 10.1096/fj.201500018R PG 8 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA DQ5MM UT WOS:000379249500009 PM 27033262 ER PT J AU Richards, J Chao, SM Friesen, T Brueggeman, R AF Richards, Jonathan Chao, Shiaoman Friesen, Timothy Brueggeman, Robert TI Fine Mapping of the Barley Chromosome 6H Net Form Net Blotch Susceptibility Locus SO G3-GENES GENOMES GENETICS LA English DT Article DE high-resolution mapping; inverse gene-for-gene; necrotrophic specialist ID TERES F-TERES; EFFECTOR-TRIGGERED SUSCEPTIBILITY; HORDEUM-VULGARE L.; PYRENOPHORA-TERES; RESISTANCE GENES; MAJOR GENES; DISEASE; POPULATION; MARKERS; REGION AB Net form net blotch, caused by the necrotrophic fungal pathogen Pyrenophora teres f. teres, is a destructive foliar disease of barley with the potential to cause significant yield loss in major production regions throughout the world. The complexity of the host-parasite genetic interactions in this pathosystem hinders the deployment of effective resistance in barley cultivars, warranting a deeper understanding of the interactions. Here, we report on the high-resolution mapping of the dominant susceptibility locus near the centromere of chromosome 6H in the barley cultivars Rika and Kombar, which are putatively targeted by necrotrophic effectors from P. teres f. teres isolates 6A and 15A, respectively. Utilization of progeny isolates derived from a cross of P. teres f. teres isolates 6A x 15A harboring single major virulence loci ( VK1, VK2, and VR2) allowed for the Mendelization of single inverse gene-for-gene interactions in a high-resolution population consisting of 2976 Rika x Kombar recombinant gametes. Brachypodium distachyon synteny was exploited to develop and saturate the susceptibility region with markers, delimiting it to similar to 0.24 cM and a partial physical map was constructed. This genetic and physical characterization further resolved the dominant susceptibility locus, designated Spt1 ( susceptibility to P. teres f. teres). The high-resolution mapping and cosegregation of the Spt1.R and Spt1.K gene/s indicates tightly linked genes in repulsion or alleles possibly targeted by different necrotrophic effectors. Newly developed barley genomic resources greatly enhance the efficiency of positional cloning efforts in barley, as demonstrated by the Spt1 fine mapping and physical contig identification reported here. C1 [Richards, Jonathan; Friesen, Timothy; Brueggeman, Robert] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Chao, Shiaoman; Friesen, Timothy] ARS, USDA, Red River Valley Agr Res Ctr, Cereal Crops Res Unit, Fargo, ND 58102 USA. RP Brueggeman, R (reprint author), N Dakota State Univ, Walster 306, Fargo, ND 58108 USA. EM Robert.Brueggeman@ndsu.edu FU National Research Initiative Competitive Grants Coordinated Agricultural Project (CAP); USDA National Institute of Food and Agriculture (NIFA) [2011-68002-30029]; National Science Foundation (NSF) North Dakota Experimental Program to Stimulate Competitive Research (NDEPSCoR) [11A-1355466]; NSF [1253987] FX The authors would like to thank Danielle Holmes for technical assistance at the United States Department of Agriculture Agricultural Research Service (USDA-ARS), Cereal Crops Research Unit, Fargo, ND. This project was supported by the National Research Initiative Competitive Grants Coordinated Agricultural Project (CAP), project 2011-68002-30029 from the USDA National Institute of Food and Agriculture (NIFA), the National Science Foundation (NSF) North Dakota Experimental Program to Stimulate Competitive Research (NDEPSCoR), Track 1 grant 11A-1355466, and the NSF CAREER project award number 1253987. NR 47 TC 2 Z9 2 U1 4 U2 8 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD JUL 1 PY 2016 VL 6 IS 7 BP 1809 EP 1818 DI 10.1534/g3.116.028902 PG 10 WC Genetics & Heredity SC Genetics & Heredity GA DR0IC UT WOS:000379590200003 PM 27172206 ER PT J AU Wang, Q Dong, B Firrman, J Wu, W Roberts, S Moore, AR Liu, LS Chin, MPS Diao, Y Kost, J Xiao, W AF Wang, Q. Dong, B. Firrman, J. Wu, W. Roberts, S. Moore, A. R. Liu, L. S. Chin, M. P. S. Diao, Y. Kost, J. Xiao, W. TI Evaluation of the biological differences of canine and human factor VIII in gene delivery: implications in human hemophilia treatment SO GENE THERAPY LA English DT Article ID ADENOASSOCIATED VIRAL VECTORS; HIGH-LEVEL EXPRESSION; A MICE; INHIBITOR DEVELOPMENT; PHENOTYPIC CORRECTION; HEAVY-CHAIN; LIGHT-CHAIN; THERAPY; FVIII; PHARMACOKINETICS AB The canine is the most important large animal model for testing novel hemophilia A (HA) treatment. It is often necessary to use canine factor VIII (cFIII) gene or protein for the evaluation of HA treatment in the canine model. However, different biological properties between cFVIII and human FVIII (hFVIII) indicated that the development of novel HA treatment may require careful characterization of non-human FVIII. To investigate whether the data obtained using cFVIII can translate to HA treatment in human, we analyzed the differential biological properties of canine heavy chain (cHC) and light chain (cLC) by comparing with human heavy chain (hHC) and light chain (hLC). The secretion of cHC was 5-30-fold higher than hHC, with or without light chains (LCs). cHC + hLC group exhibited similar to 18-fold increase in coagulation activity compared with hHC+hLC delivery by recombinant adeno-associated viral vectors. Unlike hHC, the secretion of cHC was independent of LCs. cLC improves the specific activity of FVIII by two-to threefold compared with hLC. Moreover, the cLC, but not cHC, contributes to the higher stability of cFVIII. Our results suggested that the cFVIII expression results in the canine model should be interpreted with caution as the cHC secreted more efficiently than hHC and cLC exhibited a more active and stable phenotype than hLC. C1 [Wang, Q.; Chin, M. P. S.; Diao, Y.; Xiao, W.] Huaqiao Univ, Sch Biomed Sci, Quanzhou, Fujian, Peoples R China. [Wang, Q.; Dong, B.; Roberts, S.; Moore, A. R.; Xiao, W.] Temple Univ, Sol Sherry Thrombosis Res Ctr, Philadelphia, PA 19122 USA. [Firrman, J.; Xiao, W.] Temple Univ, Dept Microbiol & Immunol, Philadelphia, PA 19122 USA. [Firrman, J.; Liu, L. S.] ARS, USDA, ERRC, Wyndmoor, PA USA. [Wu, W.; Xiao, W.] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Lab Med, Shanghai, Peoples R China. [Kost, J.] Ben Gurion Univ Negev, Dept Chem Engn, Beer Sheva, Israel. [Xiao, W.] Temple Univ, Cardiovasc Res Ctr, Philadelphia, PA 19122 USA. RP Xiao, W (reprint author), Temple Univ, Sch Med, Cardiovasc Res Ctr, Dept Microbiol & Immunol,Sol Sherry Thrombosis Re, 3440 North Broad St,MRB560, Philadelphia, PA 19140 USA. EM wxiao@temple.edu FU National Institutes of Health (NIH) [R01HL114152, R01HL080789]; Novo Nordisk Haemophilia Research Fund (NNHRF) China FX This work was supported by National Institutes of Health (NIH) (R01HL114152 and R01HL080789 to WX). The work was also supported in part by grant funding from Novo Nordisk Haemophilia Research Fund (NNHRF) China. NR 32 TC 0 Z9 0 U1 9 U2 9 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0969-7128 EI 1476-5462 J9 GENE THER JI Gene Ther. PD JUL PY 2016 VL 23 IS 7 BP 597 EP 605 DI 10.1038/gt.2016.34 PG 9 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine GA DR0GY UT WOS:000379587200007 PM 27064790 ER PT J AU Zuo, T Zhang, JB Lithio, A Dash, S Weber, DF Wise, R Nettleton, D Peterson, T AF Zuo, Tao Zhang, Jianbo Lithio, Andrew Dash, Sudhansu Weber, David F. Wise, Roger Nettleton, Dan Peterson, Thomas TI Genes and Small RNA Transcripts Exhibit Dosage-Dependent Expression Pattern in Maize Copy-Number Alterations SO GENETICS LA English DT Article DE dosage effect; copy-number alterations; phenotypic variations; small RNAs; maize ID GENOME-WIDE PATTERNS; LONG NONCODING RNAS; DROSOPHILA-MELANOGASTER; STATISTICAL-METHODS; ANEUPLOID SYNDROMES; X-CHROMOSOME; MOUSE MODEL; C-MYC; COMPENSATION; DIVERSITY AB Copy-number alterations are widespread in animal and plant genomes, but their immediate impact on gene expression is still unclear. In animals, copy-number alterations usually exhibit dosage effects, except for sex chromosomes which tend to be dosage compensated. In plants, genes within small duplications (<100 kb) often exhibit dosage-dependent expression, whereas large duplications (>50 Mb) are more often dosage compensated. However, little or nothing is known about expression in moderately-sized (150 Mb) segmental duplications, and about the response of small RNAs to dosage change. Here, we compared maize (Zea mays) plants with two, three, and four doses of a 14.6-Mb segment of chromosome 1 that contains similar to 300 genes. Plants containing the duplicated segment exhibit dosage-dependent effects on ear length and flowering time. Transcriptome analyses using GeneChip and RNA-sequencing methods indicate that most expressed genes and unique small RNAs within the duplicated segments exhibit dosage-dependent transcript levels. We conclude that dosage effect is the predominant regulatory response for both genes and unique small RNA transcripts in the segmental dosage series we tested. To our knowledge this is the first analysis of small RNA expression in plant gene dosage variants. Because segmental duplications comprise a significant proportion of eukaryotic genomes, these findings provide important new insight into the regulation of genes and small RNAs in response to dosage changes. C1 [Zuo, Tao; Zhang, Jianbo; Peterson, Thomas] Iowa State Univ, Dept Agron, Dept Genet Dev & Cell Biol, Ames, IA 50011 USA. [Lithio, Andrew; Nettleton, Dan] Iowa State Univ, Dept Stat, Ames, IA 50011 USA. [Wise, Roger] Iowa State Univ, Dept Plant Pathol & Microbiol, Ames, IA 50011 USA. [Dash, Sudhansu] Iowa State Univ, Virtual Real Applicat Ctr, Ames, IA 50011 USA. [Wise, Roger] Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Weber, David F.] Illinois State Univ, Sch Biol Sci, Normal, IL 61790 USA. RP Peterson, T (reprint author), Iowa State Univ, Mol Biol 2258, Ames, IA 50011 USA. EM thomasp@iastate.edu NR 98 TC 0 Z9 0 U1 4 U2 5 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 0016-6731 EI 1943-2631 J9 GENETICS JI Genetics PD JUL PY 2016 VL 203 IS 3 BP 1133 EP + DI 10.1534/genetics.116.188235 PG 28 WC Genetics & Heredity SC Genetics & Heredity GA DQ8PM UT WOS:000379473600011 PM 27129738 ER PT J AU Nice, LM Steffenson, BJ Brown-Guedira, GL Akhunov, ED Liu, C Kono, TJY Morrell, PL Blake, TK Horsley, RD Smith, KP Muehlbauer, GJ AF Nice, Liana M. Steffenson, Brian J. Brown-Guedira, Gina L. Akhunov, Eduard D. Liu, Chaochih Kono, Thomas J. Y. Morrell, Peter L. Blake, Thomas K. Horsley, Richard D. Smith, Kevin P. Muehlbauer, Gary J. TI Development and Genetic Characterization of an Advanced Backcross-Nested Association Mapping (AB-NAM) Population of Wild x Cultivated Barley SO GENETICS LA English DT Article DE wild barley; advanced backcross; nested association mapping population; association mapping; plant genetic resources; Multiparent Advanced Generation Inter-Cross (MAGIC); multiparental populations; MPP ID SINGLE NUCLEOTIDE POLYMORPHISMS; GENOME-WIDE ASSOCIATION; MULTIPLE DISEASE RESISTANCE; QUANTITATIVE TRAIT LOCI; VULGARE SSP SPONTANEUM; HORDEUM-VULGARE; LINKAGE DISEQUILIBRIUM; FLOWERING TIME; QTL ANALYSIS; AGRONOMIC TRAITS AB The ability to access alleles from unadapted germplasm collections is a long-standing problem for geneticists and breeders. Here we developed, characterized, and demonstrated the utility of a wild barley advanced backcross-nested association mapping (AB-NAM) population. We developed this population by backcrossing 25 wild barley accessions to the six-rowed malting barley cultivar Rasmusson. The 25 wild barley parents were selected from the 318 accession Wild Barley Diversity Collection (WBDC) to maximize allelic diversity. The resulting 796 BC2F4:6 lines were genotyped with 384 SNP markers, and an additional 4022 SNPs and 263,531 sequence variants were imputed onto the population using 9K iSelect SNP genotypes and exome capture sequence of the parents, respectively. On average, 96% of each wild parent was introgressed into the Rasmusson background, and the population exhibited low population structure. While linkage disequilibrium (LD) decay (r(2) = 0.2) was lowest in the WBDC (0.36 cM), the AB-NAM (9.2 cM) exhibited more rapid LD decay than comparable advanced backcross (28.6 cM) and recombinant inbred line (32.3 cM) populations. Three qualitative traits: glossy spike, glossy sheath, and black hull color were mapped with high resolution to loci corresponding to known barley mutants for these traits. Additionally, a total of 10 QTL were identified for grain protein content. The combination of low LD, negligible population structure, and high diversity in an adapted background make the AB-NAM an important tool for high-resolution gene mapping and discovery of novel allelic variation using wild barley germplasm. C1 [Nice, Liana M.; Liu, Chaochih; Kono, Thomas J. Y.; Morrell, Peter L.; Smith, Kevin P.; Muehlbauer, Gary J.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Steffenson, Brian J.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Horsley, Richard D.] Univ Minnesota, Dept Plant Biol, St Paul, MN 55108 USA. [Brown-Guedira, Gina L.] North Carolina State Univ, USDA ARS, Raleigh, NC 27607 USA. [Akhunov, Eduard D.] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Blake, Thomas K.] Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. [Horsley, Richard D.] North Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. RP Muehlbauer, GJ (reprint author), Univ Minnesota, Dept Plant Biol, Ctr Biol Sci 250, St Paul, MN 55108 USA. EM muehl003@umn.edu FU United States Department of Agriculture (USDA) National Needs Fellowship [2007-38420-17749]; USDA Triticeae Coordinated Agricultural Project [2011-68002-30029] FX We thank Shane Heinen for greenhouse support and Ed Schiefelbein, Guillermo Velasquez, and Martin Hochhalter for field management. Funding provided by the United States Department of Agriculture (USDA) National Needs Fellowship 2007-38420-17749 and USDA Triticeae Coordinated Agricultural Project 2011-68002-30029. NR 81 TC 1 Z9 1 U1 11 U2 14 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 0016-6731 EI 1943-2631 J9 GENETICS JI Genetics PD JUL PY 2016 VL 203 IS 3 BP 1453 EP + DI 10.1534/genetics.116.190736 PG 29 WC Genetics & Heredity SC Genetics & Heredity GA DQ8PM UT WOS:000379473600032 PM 27182953 ER PT J AU Robins, JG AF Robins, Joseph G. TI Evaluation of warm-season grasses nutritive value as alternatives to cool-season grasses under limited irrigation SO GRASSLAND SCIENCE LA English DT Article DE Crude protein; digestibility; genotype by environment; warm season grasses; water soluble carbohydrates ID SWITCHGRASS POPULATIONS; QUALITY; YIELD; ETHANOL; BIOMASS AB The production of cool-season grasses is limited by their photosynthetic inefficiency during the hot summer months. Therefore, a study was conducted during 2006 and 2007 at a Logan, UT, USA field site to determine the potential of various warm-season grasses as alternatives to cool-season grasses during summer under limited irrigation. The study included 20 environments, which corresponded to combinations of the 2years, two harvest dates (June and July) and five irrigation levels. There were differences among the 27 varieties (21 warm-season and six cool-season) for crude protein (CP), invitro true digestibility (IVTD), neutral detergent fiber (NDF), neutral detergent fiber digestibility (NDFD), water soluble carbohydrates (WSC) and biomass (BM) across the 20 environments. However, the overall variety effects were ameliorated by the presence of genotype by environment interaction for CP, WSC, BM, and to a lesser extent IVTD. The cool-season grasses generally possessed higher trait values in each environment, yet there were several warm-season grasses, such as big bluestem (Andropogon gerardii Vitman) variety Bison' and switchgrass (Panicum virgatum L.) variety Trailblazer' that possessed higher values for the nutritive value traits. Additionally, these warm-season grass varieties possessed substantially higher BM than any cool-season grass varieties. Thus, there are warm-season grass varieties that combine high BM and higher nutritive value in the summer months under limited irrigation. These may prove to be viable forage alternatives to the cool-season grasses during the summer slump period. C1 [Robins, Joseph G.] Utah State Univ, USDA ARS, Forage & Range Res Lab, Dept Plants Soils & Climate, Logan, UT 84322 USA. RP Robins, JG (reprint author), Utah State Univ, ARS, Forage & Range Res, 6300 Old Main Hill, Logan, UT 84322 USA. EM joseph.robins@ars.usda.gov FU Utah Agricultural Experiment Station Pasture Initiative FX The author thanks Ramsey Buffham, Steve Smith, Dave Mabey, and numerous student employees for assistance on this research. The Utah Agricultural Experiment Station Pasture Initiative provided funding that supported the research in part. NR 21 TC 0 Z9 0 U1 3 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1744-6961 EI 1744-697X J9 GRASSL SCI JI Grassl. Sci. PD JUL PY 2016 VL 62 IS 3 BP 144 EP 150 DI 10.1111/grs.12123 PG 7 WC Agriculture, Multidisciplinary; Agronomy SC Agriculture GA DR4WZ UT WOS:000379905300002 ER PT J AU Burakowski, EA Ollinger, SV Bonan, GB Wake, CP Dibb, JE Hollinger, DY AF Burakowski, E. A. Ollinger, S. V. Bonan, G. B. Wake, C. P. Dibb, J. E. Hollinger, D. Y. TI Evaluating the Climate Effects of Reforestation in New England Using a Weather Research and Forecasting (WRF) Model Multiphysics Ensemble SO JOURNAL OF CLIMATE LA English DT Article ID CONTERMINOUS UNITED-STATES; STABLE BOUNDARY-LAYER; SNOW-COVERED PERIODS; LAND-USE; SURFACE ALBEDO; TEMPERATURE-CHANGE; BOREAL FOREST; MODIS DATA; PRECIPITATION; SIMULATIONS AB The New England region of the northeastern United States has a land use history characterized by forest clearing for agriculture and other uses during European colonization and subsequent reforestation following widespread farm abandonment. Despite these broad changes, the potential influence on local and regional climate has received relatively little attention. This study investigated wintertime (December through March) climate impacts of reforestation in New England using a high-resolution (4 km) multiphysics ensemble of the Weather Research and Forecasting Model. In general, the conversion from mid-1800s cropland/grassland to forest led to warming, but results were sensitive to physics parameterizations. The 2-m maximum temperature (T2max) was most sensitive to choice of land surface model, 2-m minimum temperature (T2min) was sensitive to radiation scheme, and all ensemble members simulated precipitation poorly. Reforestation experiments suggest that conversion of mid-1800s cropland/grassland to present-day forest warmed T2max +0.5 to +3K, with weaker warming during a warm, dry winter compared to a cold, snowy winter. Warmer T2max over forests was primarily the result of increased absorbed shortwave radiation and increased sensible heat flux compared to cropland/grassland. At night, T2min warmed +0.2 to +1.5K where deciduous broadleaf forest replaced cropland/grassland, a result of decreased ground heat flux. By contrast, T2min of evergreen needleleaf forest cooled -0.5 to -2.1 K, primarily owing to increased ground heat flux and decreased sensible heat flux. C1 [Burakowski, E. A.; Ollinger, S. V.; Wake, C. P.; Dibb, J. E.] Univ New Hampshire, Earth Syst Res Ctr, Durham, NH 03824 USA. [Burakowski, E. A.; Bonan, G. B.] Natl Ctr Atmospher Res, POB 3000, Boulder, CO 80307 USA. [Hollinger, D. Y.] US Forest Serv, USDA, Northern Res Stn, Durham, NH USA. RP Burakowski, EA (reprint author), Natl Ctr Atmospher Res, Climate & Global Dynam Div, 1850 Table Mesa Dr,ML 174b, Boulder, CO 80305 USA. EM burakows@ucar.edu OI Burakowski, Elizabeth/0000-0001-5429-9886 FU National Science Foundation Experimental Program to Stimulate Competitive Research (EPSCoR); New Hampshire EPSCoR Ecosystems and Society Program [EPS-1101245]; NASA Terrestrial Ecology Program [NNX12AK56G S01]; Northern Ecosystems Research Cooperative (NERC); National Science Foundation [EF-1048481]; NSF FX The authors acknowledge the support of the National Science Foundation Experimental Program to Stimulate Competitive Research (EPSCoR), the New Hampshire EPSCoR Ecosystems and Society Program (EPS-1101245), the NASA Terrestrial Ecology Program (NNX12AK56G S01), the Northern Ecosystems Research Cooperative (NERC), and National Science Foundation Grant EF-1048481. This study is contribution of the NSF-supported Harvard Forest and Hubbard Brook Long-Term Ecological Research projects. We would also like to recognize the computational support provided by the NCAR Computational and Information Systems Laboratory for computing, processing, and data storage. We thank two anonymous reviewers for their contributions toward improving the manuscript. NR 69 TC 0 Z9 0 U1 7 U2 10 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 0894-8755 EI 1520-0442 J9 J CLIMATE JI J. Clim. PD JUL PY 2016 VL 29 IS 14 BP 5141 EP 5156 DI 10.1175/JCLI-D-15-0286.1 PG 16 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DQ4YD UT WOS:000379209800005 ER PT J AU Galindo-Leva, LA Hughes, SR Lopez-Nunez, JC Jarodsky, JM Erickson, A Lindquist, MR Cox, EJ Bischoff, KM Hoecker, EC Liu, SQ Qureshi, N Jones, MA AF Galindo-Leva, Luz Angela Hughes, Stephen R. Carlos Lopez-Nunez, Juan Jarodsky, Joshua M. Erickson, Adam Lindquist, Mitchell R. Cox, Elby J. Bischoff, Kenneth M. Hoecker, Eric C. Liu, Siqing Qureshi, Nasib Jones, Marjorie A. TI Growth, ethanol production, and inulinase activity on various inulin substrates by mutant Kluyveromyces marxianus strains NRRL Y-50798 and NRRL Y-50799 SO JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY LA English DT Article DE Kluyveromyces marxianus; Inulin; Inulinase; Coffee waste fermentation; Biorefinery platform ID COFFEE BY-PRODUCTS; JERUSALEM-ARTICHOKE; FERMENTATION; OLIGOSACCHARIDES; RAFFINOSE; FRUCTOSE; BIOMASS; WASTES; SUGARS AB Economically important plants contain large amounts of inulin. Disposal of waste resulting from their processing presents environmental issues. Finding microorganisms capable of converting inulin waste to biofuel and valuable co-products at the processing site would have significant economic and environmental impact. We evaluated the ability of two mutant strains of Kluyveromyces marxianus (Km7 and Km8) to utilize inulin for ethanol production. In glucose medium, both strains consumed all glucose and produced 0.40 g ethanol/g glucose at 24 h. In inulin medium, Km7 exhibited maximum colony forming units (CFU)/mL and produced 0.35 g ethanol/g inulin at 24 h, while Km8 showed maximum CFU/mL and produced 0.02 g ethanol/g inulin at 96 h. At 24 h in inulin + glucose medium, Km7 produced 0.40 g ethanol/g ( inulin + glucose) and Km8 produced 0.20 g ethanol/g ( inulin + glucose) with maximum CFU/mL for Km8 at 72 h, 40 % of that for Km7 at 36 h. Extracellular inulinase activity at 6 h for both Km7 and Km8 was 3.7 International Units ( IU)/mL. C1 [Galindo-Leva, Luz Angela; Jarodsky, Joshua M.; Erickson, Adam; Jones, Marjorie A.] Illinois State Univ, Dept Chem, Normal, IL 61790 USA. [Hughes, Stephen R.; Lindquist, Mitchell R.; Cox, Elby J.; Bischoff, Kenneth M.; Hoecker, Eric C.; Liu, Siqing; Qureshi, Nasib] ARS, USDA, NCAUR, Renewable Prod Technol Res Unit, 1815 North Univ St, Peoria, IL 61604 USA. [Carlos Lopez-Nunez, Juan] Natl Federat Coffee Growers Colombia FNC, Natl Coffee Res Ctr Cenicafe, Cenicafe Planalto Km 4 Via Antigua Chinchina, Caldas, Colombia. RP Hughes, SR (reprint author), ARS, USDA, NCAUR, Renewable Prod Technol Res Unit, 1815 North Univ St, Peoria, IL 61604 USA. EM stephen.hughes@ars.usda.gov FU Organization of American States FX The authors gratefully acknowledge the financial support of the Organization of American States for this research work. NR 38 TC 1 Z9 1 U1 10 U2 14 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1367-5435 EI 1476-5535 J9 J IND MICROBIOL BIOT JI J. Ind. Microbiol. Biotechnol. PD JUL PY 2016 VL 43 IS 7 BP 927 EP 939 DI 10.1007/s10295-016-1771-5 PG 13 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DR0VS UT WOS:000379625600004 PM 27130462 ER PT J AU Sun, Y Koh, HWL Choi, H Koh, WP Yuan, JM Newman, JW Su, J Fang, JL Ong, CN van Dam, RM AF Sun, Ye Koh, Hiromi W. L. Choi, Hyungwon Koh, Woon-Puay Yuan, Jian-Min Newman, John W. Su, Jin Fang, Jinling Ong, Choon Nam van Dam, Rob M. TI Plasma fatty acids, oxylipins, and risk of myocardial infarction: the Singapore Chinese Health Study SO JOURNAL OF LIPID RESEARCH LA English DT Article DE epidemiology; lipidomics; heart; fatty acid/metabolism; eicosanoids; lipids; oxidized lipids; diet and dietary lipids; nutrition; mass spectrometry ID CORONARY-HEART-DISEASE; DIETARY LINOLEIC-ACID; ARACHIDONIC-ACID; ARTERY-DISEASE; CARDIOVASCULAR-DISEASE; ADIPOSE-TISSUE; STEARIC-ACID; METAANALYSIS; EICOSANOIDS; WOMEN AB We aimed to examine the prospective association between plasma FAs, oxylipins, and risk of acute myocardial infarction (AMI) in a Singapore Chinese population. A nested case-control study with 744 incident AMI cases and 744 matched controls aged 47-83 years was conducted within the Singapore Chinese Health Study. Nineteen plasma FAs and 12 oxylipins were quantified using MS. These were grouped into 12 FA clusters and 5 oxylipin clusters using hierarchical clustering, and their associations with AMI risk were assessed. Long-chain n-3 FAs [odds ratio (OR) = 0.67 per SD increase, 95% confidence interval (CI): 0.53-0.84, P < 0.001] and stearic acid (OR = 0.65, 95% CI: 0.44-0.97, P = 0.03) were inversely associated with AMI risk, whereas arachidonic acid (AA) was positively associated with AMI risk (OR = 1.25, 95% CI: 1.03-1.52, P = 0.02) in the multivariable model with adjustment for other FAs. Further adjustment for oxylipins did not substantially change these associations. An inverse association was observed between AA-derived oxylipin, thromboxane (TX) B-2, and AMI risk (OR = 0.81, 95% CI: 0.71-0.93, P = 0.003). Circulating long-chain n-3 FAs and stearic acid were associated with a lower and AA was associated with a higher AMI risk in this Chinese population. The association between the oxylipin TXB2 and AMI requires further research.-Sun, Y., Hiromi W. L. Koh, H. Choi, W-P. Koh, J-M. Yuan, J. W. Newman, J. Su, J. Fang, C. N. Ong, and R. M. van Dam. Plasma fatty acids, oxylipins, and risk of myocardial infarction: the Singapore Chinese Health Study. C1 [Sun, Ye; Koh, Hiromi W. L.; Choi, Hyungwon; Koh, Woon-Puay; Su, Jin; Fang, Jinling; Ong, Choon Nam; van Dam, Rob M.] Natl Univ Singapore, Yong Loo Lin Sch Med, Saw Swee Hock Sch Publ Hlth, Singapore, Singapore. [Sun, Ye] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Psychol Med, Singapore, Singapore. [van Dam, Rob M.] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Med, Singapore, Singapore. Natl Univ Hlth Syst, Singapore, Singapore. [Sun, Ye; van Dam, Rob M.] Natl Univ Singapore, NUS Grad Sch Integrat Sci & Engn, Singapore, Singapore. [Ong, Choon Nam] Natl Univ Singapore, Natl Univ Singapore Environm Res Inst, Singapore, Singapore. [Koh, Woon-Puay] Duke NUS Grad Med Sch Singapore, Singapore, Singapore. [Yuan, Jian-Min] Univ Pittsburgh, Grad Sch Publ Hlth, Univ Pittsburgh Canc Inst, Div Canc Control & Populat Sci, Pittsburgh, PA USA. [Yuan, Jian-Min] Univ Pittsburgh, Grad Sch Publ Hlth, Dept Epidemiol, Pittsburgh, PA USA. [Newman, John W.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Newman, John W.] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. [van Dam, Rob M.] Harvard TH Chan Sch Publ Hlth, Dept Nutr, Boston, MA USA. RP van Dam, RM (reprint author), Natl Univ Singapore, Yong Loo Lin Sch Med, Saw Swee Hock Sch Publ Hlth, Singapore, Singapore.; van Dam, RM (reprint author), Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Med, Singapore, Singapore.; van Dam, RM (reprint author), Natl Univ Singapore, NUS Grad Sch Integrat Sci & Engn, Singapore, Singapore.; van Dam, RM (reprint author), Harvard TH Chan Sch Publ Hlth, Dept Nutr, Boston, MA USA. EM rob_martinus_van_dam@nuhs.edu.sg FU Office of Extramural Research, National Institutes of Health [R01 CA144034, UM1 CA182876]; National Medical Research Council, Singapore [NMRC/1270/2010]; U.S. Department of Agriculture [23032-51530-022-00D] FX This study was supported by Office of Extramural Research, National Institutes of Health Grants R01 CA144034 and UM1 CA182876, and National Medical Research Council, Singapore (NMRC/1270/2010). Additional support was provided by U.S. Department of Agriculture Intramural Project (23032-51530-022-00D to J.W.N). The US Department of Agriculture is an equal opportunity provider and employer. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. NR 37 TC 1 Z9 1 U1 3 U2 6 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0022-2275 EI 1539-7262 J9 J LIPID RES JI J. Lipid Res. PD JUL PY 2016 VL 57 IS 7 BP 1300 EP 1307 DI 10.1194/jlr.P066423 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA DR1GJ UT WOS:000379653700018 PM 27371261 ER PT J AU Ng, K Stoll, B Chacko, S de Pipaon, MS Lauridsen, C Gray, M Squires, EJ Marini, J Zamora, IJ Olutoye, OO Burrin, DG AF Ng, Kenneth Stoll, Barbara Chacko, Shaji Saenz de Pipaon, Miguel Lauridsen, Charlotte Gray, Matthew Squires, E. James Marini, Juan Zamora, Irving J. Olutoye, Oluyinka O. Burrin, Douglas G. TI Vitamin E in New-Generation Lipid Emulsions Protects Against Parenteral Nutrition-Associated Liver Disease in Parenteral Nutrition-Fed Preterm Pigs SO JOURNAL OF PARENTERAL AND ENTERAL NUTRITION LA English DT Article DE neonates; life cycle; liver disease; research and diseases; phytosterols; nuclear receptors; cholestasis ID PREGNANE-X-RECEPTOR; BILE-ACID-HOMEOSTASIS; INTESTINAL FAILURE; PLANT STEROLS; FISH-OIL; NUCLEAR RECEPTORS; CHOLESTASIS; SERUM; PHYTOSTEROLS; METABOLISM AB Introduction: Parenteral nutrition (PN) in preterm infants leads to PN-associated liver disease (PNALD). PNALD has been linked to serum accumulation of phytosterols that are abundant in plant oil but absent in fish oil emulsions. Hypothesis: Whether modifying the phytosterol and vitamin E composition of soy and fish oil lipid emulsions affects development of PNALD in preterm pigs. Methods: We measured markers of PNALD in preterm pigs that received 14 days of PN that included 1 of the following: (1) Intralipid (IL, 100% soybean oil), (2) Intralipid + vitamin E (ILE, d--tocopherol), (3) Omegaven (OV, 100% fish oil), or (4) Omegaven + phytosterols (PS, -sitosterol, campesterol, and stigmasterol). Results: Serum levels of direct bilirubin, gamma glutamyl transferase, serum triglyceride, low-density lipoprotein, and hepatic triglyceride content were significantly lower (P < .05) in the ILE, OV, and PS compared to IL. Hepatic cholesterol 7-hydroxylase and organic solute transporter- expression was lower (P < .05) and portal plasma FGF19 higher in the ILE, OV, and PS vs IL. Hepatic expression of mitochondrial carnitine palmitoyltransferase 1A and microsomal cytochrome P450 2E1 fatty acid oxidation genes was higher in ILE, OV, and PS vs IL. In vivo C-13-CDCA clearance and expression of pregnane X receptor target genes, cytochrome P450 3A29 and multidrug resistance-associated protein 2, were higher in ILE, OV, and PS vs IL. Conclusions: -tocopherol in Omegaven and added to Intralipid prevented serum and liver increases in biliary and lipidemic markers of PNALD in preterm piglets. The addition of phytosterols to Omegaven did not produce evidence of PNALD. C1 [Ng, Kenneth; Burrin, Douglas G.] Baylor Coll Med, Dept Pediat, Sect Gastroenterol Hepatol & Nutr, Houston, TX 77030 USA. [Stoll, Barbara; Chacko, Shaji; Marini, Juan; Burrin, Douglas G.] USDA ARS, Childrens Nutr Res Ctr, Houston, TX USA. [Saenz de Pipaon, Miguel] La Paz Univ Hosp, Dept Neonatol, Madrid, Spain. [Lauridsen, Charlotte] Aarhus Univ, Dept Anim Sci, Tjele, Denmark. [Gray, Matthew; Squires, E. James] Univ Guelph, Dept Anim & Poultry Sci, Guelph, ON, Canada. [Zamora, Irving J.; Olutoye, Oluyinka O.] Texas Childrens Hosp, Baylor Coll Med, Michael DeBakey Dept Surg, Div Pediat Surg, Houston, TX 77030 USA. RP Burrin, DG (reprint author), USDA, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM Doug.Burrin@ars.usda.gov FU U.S. Department of Agriculture, Agricultural Research Service [58-6250-6-001]; American Society for Parenteral and Enteral Nutrition Rhoads Research Foundation; NIH [DK-094616]; Texas Medical Center Digestive Diseases Center (NIH) [P30 DK-56338]; American Liver Foundation; Instituto de Salud Carlos III [BA12/00086] FX This work is a publication of the USDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine and Texas Children's Hospital, Houston, Texas. This work was supported in part by federal funds from the U.S. Department of Agriculture, Agricultural Research Service under Cooperative Agreement Number 58-6250-6-001, the American Society for Parenteral and Enteral Nutrition Rhoads Research Foundation (to K.N.), NIH Grant DK-094616 (D.G.B.), and the Texas Medical Center Digestive Diseases Center (NIH Grant P30 DK-56338). The contents of this publication do not necessarily reflect the views or policies of the U.S. Department of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. government. Kenneth Ng was supported by a research fellowship award from the American Liver Foundation. Miguel Saenz de Pipaon was supported by a grant from the Instituto de Salud Carlos III, BA12/00086. NR 50 TC 1 Z9 1 U1 6 U2 6 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0148-6071 EI 1941-2444 J9 JPEN-PARENTER ENTER JI J. Parenter. Enter. Nutr. PD JUL PY 2016 VL 40 IS 5 BP 656 EP 671 DI 10.1177/0148607114567900 PG 16 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DR0PL UT WOS:000379609300007 PM 25596209 ER PT J AU Arthur, FH AF Arthur, F. H. TI Efficacy of methoprene for multi-year protection of stored wheat, brown rice, rough rice and corn SO JOURNAL OF STORED PRODUCTS RESEARCH LA English DT Article DE Grain; Rhyzopertha dominica; Sitotroga cerealella; Tribolium castaneum; Methoprene; Storage ID GRAIN MOTH LEPIDOPTERA; RHYZOPERTHA-DOMINICA; SITOTROGA-CEREALELLA; SUSCEPTIBILITY; RESISTANCE; VARIETIES; GELECHIIDAE; COLEOPTERA; SPINOSAD; BEETLES AB Hard red winter wheat, brown rice, rough rice, and corn were treated with the insect growth regulator (IGR) methoprene at rates of 1.25 and 2.5 ppm, held for 24 months at ambient conditions in buckets on the floor of a grain bin, and sampled every two months. Bioassays were done by exposing 10 mixed-sex adults of Rhyzopertha dominica (F.), the lesser grain borer, and Tribolium castaneum (Herbst), the red flour beetle, on wheat, R. dominica and Sitotroga cerealella (Oliver), the Angoumois grain moth, on brown rice and rough rice, and T castaneum and S. cerealella on corn. Sample size for all commodities was about 80 g, and these samples were held for 3 months at 27 degrees C-60% r.h. Both rates of the IGR completely suppressed adult progeny development of R. dominica with little resulting feeding damage, sample weight loss, or insect damaged kernels (IDK). Some adult progeny production of S. cerealella and resulting IDK occurred at both rates on rough rice, brown rice, and corn, but was far less than in untreated controls. There was little adult progeny production but some feeding damage caused by larval T castaneum in the treated wheat and corn but again far less than in untreated control. Allowing continual exposure of parental adults on grains treated with an IGR, rather than exposing those parental adults for a short time period, may give more accurate evaluations of residual efficacy. Results show that methoprene used as a grain protectant will give residual control of stored product beetles for 24 months, but complete control of S. cerealella may require inclusion of a contact insecticide. (C) 2016 Published by Elsevier Ltd. C1 [Arthur, F. H.] ARS, USDA, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. RP Arthur, FH (reprint author), ARS, USDA, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM frank.arthur@ars.usda.gov FU Central Life Sciences FX We thank Brian Barnett for excellent technical assistance throughout this study. We also thank Central Life Sciences for funding the study. This paper reports the results of research only. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The US Department of Agriculture is an equal opportunity provider and employer. NR 22 TC 0 Z9 1 U1 10 U2 11 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-474X EI 1879-1212 J9 J STORED PROD RES JI J. Stored Prod. Res. PD JUL PY 2016 VL 68 BP 85 EP 92 DI 10.1016/j.jspr.2016.04.005 PG 8 WC Entomology SC Entomology GA DR0XO UT WOS:000379630400011 ER PT J AU Taha-Abdelaziz, K Bassel, LL Harness, ML Clark, ME Register, KB Caswell, JL AF Taha-Abdelaziz, Khaled Bassel, Laura L. Harness, Melanie L. Clark, Mary Ellen Register, Karen B. Caswell, Jeff L. TI Cilia-associated bacteria in fatal Bordetella bronchiseptica pneumonia of dogs and cats SO JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION LA English DT Article DE Adhesion; Bordetella bronchiseptica; bronchopneumonia; cats; cilia; dogs; pneumonia ID RESPIRATORY-TRACT INFECTIONS; ANIMAL SHELTERS; DISEASE; PATHOGENS; COLONIZATION; PREVALENCE; MANAGEMENT; PROTEINS; VIRUSES; SWINE AB Bordetella bronchiseptica frequently causes nonfatal tracheobronchitis, but its role in fatal pneumonia is less recognized. Our study evaluated histologic identification of cilia-associated bacteria as a method for diagnosis of B. bronchiseptica pneumonia. Cases of fatal bronchopneumonia were studied retrospectively, excluding neonates and cases of aspiration pneumonia, minor lung lesions, or autolysis. The study population comprised 36 canine and 31 feline cases of bronchopneumonia. B. bronchiseptica was identified in 8 of 36 canine and 14 of 31 feline cases based on immunohistochemistry (IHC) using serum from a rabbit hyperimmunized with pertactin, PCR testing (Fla2/Fla12), and/or bacterial culture data when available. Of these, IHC was positive in 4 canine and 7 feline cases, PCR was positive in 8 canine and 14 feline cases, and B. bronchiseptica was isolated in 2 of 5 canine and 3 of 9 feline cases tested. Examination of histologic sections stained with hematoxylin and eosin revealed bronchial cilia-associated bacteria in 4 of 36 canine and 5 of 31 feline cases; these were all positive by IHC and PCR. The presence of cilia-associated bacteria had been noted in the pathology report for only 2 of these 9 cases. Thus, the presence of cilia-associated bacteria seems frequently overlooked by pathologists, but is a diagnostically significant feature of B. bronchiseptica pneumonia. A specific diagnosis of B. bronchiseptica pneumonia is important because it suggests primary or opportunistic bacterial pneumonia rather than aspiration pneumonia, and because of the risk of animal-to-animal transmission of B. bronchiseptica, the availability of vaccines for disease prevention, and the potential zoonotic risk to immunocompromised pet owners. C1 [Taha-Abdelaziz, Khaled; Bassel, Laura L.; Harness, Melanie L.; Clark, Mary Ellen; Caswell, Jeff L.] Univ Guelph, Dept Pathobiol, Guelph, ON N1G 2W1, Canada. [Taha-Abdelaziz, Khaled] Beni Suef Univ, Fac Vet Med, Dept Pathol, Bani Suwayf, Egypt. [Register, Karen B.] USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA. RP Caswell, JL (reprint author), Univ Guelph, Dept Pathobiol, Guelph, ON N1G 2W1, Canada. EM jcaswell@uoguelph.ca OI Caswell, Jeff/0000-0002-1991-3219 FU Natural Sciences and Engineering Research Council of Canada (NSERC) [RGPIN 227845] FX The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was funded by the Natural Sciences and Engineering Research Council of Canada (NSERC; RGPIN 227845 to JLC). NR 33 TC 0 Z9 0 U1 10 U2 15 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1040-6387 EI 1943-4936 J9 J VET DIAGN INVEST JI J. Vet. Diagn. Invest. PD JUL PY 2016 VL 28 IS 4 BP 369 EP 376 DI 10.1177/1040638716646806 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA DQ9LY UT WOS:000379532600003 PM 27178716 ER PT J AU Roug, A Swift, P Puschner, B Gerstenberg, G Mertins, JW Johnson, CK Torres, S Mortensen, J Woods, L AF Roug, Annette Swift, Pamela Puschner, Birgit Gerstenberg, Greg Mertins, James W. Johnson, Christine Kreuder Torres, Steve Mortensen, Jack Woods, Leslie TI Exotic pediculosis and hair-loss syndrome in deer (Odocoileus hemionus) populations in California SO JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION LA English DT Article DE Bovicola tibialis; Damalinia (Cervicola) sp.; deer; hair-loss syndrome; selenium ID BLACK-TAILED DEER; SELENIUM DEFICIENCY; TRACE-ELEMENTS; FAWN SURVIVAL; TULE ELK; COPPER; SERUM; TRICHODECTIDAE; INFESTATION; CATTLE AB Infestation with nonnative, "exotic" lice was first noted in Washington black-tailed deer (Odocoileus hemionus columbianus) in 1994 and has since then spread throughout the western United States. In California, infestation with the exotic louse Damalinia (Cervicola) sp. was first detected in black-tailed deer from northern California in 2004, and, in 2009, the exotic louse species Bovicola tibialis and Linognathus africanus were identified on mule deer (Odocoileus hemionus californicus) in central Sierra Nevada in association with a mortality event. Exotic lice have since been detected in various locations throughout the state. We describe the geographic distribution of these exotic lice within California, using data from 520 livecaptured and 9 postmortem-sampled, free-ranging mule deer examined between 2009 and 2014. Data from live-captured deer were used to assess possible associations between louse infestation and host age, host sex, migratory behavior, season, and blood selenium and serum copper concentrations. Damalinia (Cervicola) sp. and B. tibialis lice were distinctively distributed geographically, with D. (Cervicola) sp. infesting herds in northern and central coastal California, B. tibialis occurring in the central coastal mountains and the Sierra Nevada, and L. africanus occurring only sporadically. Younger age classes and low selenium concentrations were significantly associated with exotic louse infestation, whereas no significant relationship was detected with serum copper levels. Our results show that exotic lice are widespread in California, and younger age classes with low blood selenium concentrations are more likely to be infested with lice than older deer. C1 [Roug, Annette; Johnson, Christine Kreuder] Univ Calif Davis, Sch Vet Med, Wildlife Hlth Ctr, 620 W Hlth Sci Dr, Davis, CA 95616 USA. [Puschner, Birgit] Univ Calif Davis, Sch Vet Med, Dept Mol Biosci, 620 W Hlth Sci Dr, Davis, CA 95616 USA. [Roug, Annette] Utah Div Wildlife Resources, Salt Lake City, UT USA. [Swift, Pamela; Torres, Steve] Calif Dept Fish & Wildlife, Rancho Cordova, CA USA. [Gerstenberg, Greg] Calif Dept Fish & Wildlife, Fresno, CA USA. [Mertins, James W.] US Anim & Plant Hlth Inspect Serv, USDA, Vet Serv Sci Technol & Anal Serv, Natl Vet Serv Labs, Ames, IA USA. [Mortensen, Jack] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Woods, Leslie] Univ Calif Davis, Sch Vet Med, Calif Anim Hlth & Food Safety Lab, 620 W Hlth Sci Dr, Davis, CA 95616 USA. RP Woods, L (reprint author), Univ Calif Davis, Sch Vet Med, Calif Anim Hlth & Food Safety Lab, 620 W Hlth Sci Dr, Davis, CA 95616 USA. EM lwwoods@ucdavis.edu FU California Department of Fish and Wildlife FX The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was funded by the California Department of Fish and Wildlife. NR 28 TC 0 Z9 0 U1 1 U2 4 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1040-6387 EI 1943-4936 J9 J VET DIAGN INVEST JI J. Vet. Diagn. Invest. PD JUL PY 2016 VL 28 IS 4 BP 399 EP 407 DI 10.1177/1040638716647154 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA DQ9LY UT WOS:000379532600007 PM 27240567 ER PT J AU Guo, L Zhao, GY Xu, JR Kistler, HC Gao, LX Ma, LJ AF Guo, Li Zhao, Guoyi Xu, Jin-Rong Kistler, H. Corby Gao, Lixin Ma, Li-Jun TI Compartmentalized gene regulatory network of the pathogenic fungus Fusarium graminearum SO NEW PHYTOLOGIST LA English DT Article DE Bayesian network inference; cell circuits; Fusarium graminearum; modularity; network rewire and fungal pathogenesis ID CAMP-PKA PATHWAY; GIBBERELLA-ZEAE; EXPRESSION DATA; SACCHAROMYCES-CEREVISIAE; PERITHECIUM DEVELOPMENT; TRANSCRIPTION FACTORS; MOLECULAR-MECHANISMS; CONIDIAL GERMINATION; REVEALS; VERTICILLIOIDES AB Head blight caused by Fusarium graminearum threatens world-wide wheat production, resulting in both yield loss and mycotoxin contamination. We reconstructed the global F. graminearum gene regulatory network (GRN) from a large collection of transcriptomic data using Bayesian network inference, a machine-learning algorithm. This GRN reveals connectivity between key regulators and their target genes. Focusing on key regulators, this network contains eight distinct but interwoven modules. Enriched for unique functions, such as cell cycle, DNA replication, transcription, translation and stress responses, each module exhibits distinct expression profiles. Evolutionarily, the F. graminearum genome can be divided into core regions shared with closely related species and variable regions harboring genes that are unique to F. graminearum and perform species-specific functions. Interestingly, the inferred top regulators regulate genes that are significantly enriched from the same genomic regions (P < 0.05), revealing a compartmentalized network structure that may reflect network rewiring related to specific adaptation of this plant pathogen. This first-ever reconstructed filamentous fungal GRN primes our understanding of pathogenicity at the systems biology level and provides enticing prospects for novel disease control strategies involving the targeting of master regulators in pathogens. The program can be used to construct GRNs of other plant pathogens. C1 [Guo, Li; Ma, Li-Jun] Univ Massachusetts, Dept Biochem & Mol Biol, Amherst, MA 01003 USA. [Zhao, Guoyi; Gao, Lixin] Univ Massachusetts, Dept Elect & Comp Engn, Amherst, MA 01003 USA. [Xu, Jin-Rong] Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA. [Kistler, H. Corby] Univ Minnesota, USDA, ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Ma, LJ (reprint author), Univ Massachusetts, Dept Biochem & Mol Biol, Amherst, MA 01003 USA. EM lijun@biochem.umass.edu OI Guo, Li/0000-0001-6100-3481 FU National Research Initiative Competitive Grants Program from the USDA National Institute of Food and Agriculture [2008-35604-18800, MASR-2009-04374]; Massachusetts Green High Performance Computing Center; National Research Initiative Hatch Grants Program [MAS00441] FX This project was supported by the National Research Initiative Competitive Grants Program grant nos. 2008-35604-18800 and MASR-2009-04374 from the USDA National Institute of Food and Agriculture. L. Guo and L-J.M. were also supported by a seed grant from Massachusetts Green High Performance Computing Center and the National Research Initiative Hatch Grants Program grant no. MAS00441. The authors would like to extend their thanks to Steve Klosterman, Lynda Ciuffitti, Ralph Dean and Andrew Breakspear for providing DNA and RNA samples; to Supriya Gupta and Andrew Crenshaw at the Broad Institute Expression Platform for conducting hybridization of both DNA and RNA samples to the Affymetrix arrays and for processing the microarray results; and to Gene Tanimoto and Stan Trask at Affymetrix for their assistance with the design of the fungal pathogen microarrays and for optimizing the hybridization conditions. NR 71 TC 1 Z9 1 U1 12 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD JUL PY 2016 VL 211 IS 2 BP 527 EP 541 DI 10.1111/nph.13912 PG 15 WC Plant Sciences SC Plant Sciences GA DQ4ZI UT WOS:000379213200016 PM 26990214 ER PT J AU Eiden, AL Kaufman, PE Allan, SA Oi, F AF Eiden, Amanda L. Kaufman, Phillip E. Allan, Sandra A. Oi, Faith TI Establishing the discriminating concentration for permethrin and fipronil resistance in Rhipicephalus sanguineus (Latreille) (Acari: Ixodidae), the brown dog tick SO PEST MANAGEMENT SCIENCE LA English DT Article DE Rhipicephalus sanguineus; acaricide resistance; diagnostic concentration; permethrin; fipronil ID BOOPHILUS-MICROPLUS ACARI; INSECTICIDE; TESTS; LC50 AB BACKGROUND: Rhipicephalus sanguineus (Latreille), the brown dog tick, is a veterinary canine and urban pest. These ticks have been found to develop resistance and tolerance to the two commonly used acaricides permethrin and fipronil respectively. We have developed a discriminating concentration that can be used for rapid detection of permethrin and fipronil resistance in brown dog tick populations. The availability of a discriminating concentration for the brown dog tick provides an inexpensive and rapid resistance diagnostic technique that can be used to guide tick management plans for companion animals and aid in the selection of environmental treatment options. RESULTS: Establishing the discriminating concentration for permethrin and fipronil in brown dog ticks enables a resistant: susceptible screening. For permethrin the discriminating concentration was set at 0.19%, and for fipronil at 0.15%. Three additional diagnostic concentrations were chosen to evaluate resistance levels when larval tick numbers were available for screening. CONCLUSION: Future tick submissions from residences and kennel facilities can be subjected to a single chemical concentration to diagnose resistance, which minimizes time, costs and tick rearing requirements and guides effective control plans. With the standardized use of larval ticks, a client-submission quantity of ideally five engorged females would provide sufficient larval numbers to utilize this technique. (C) 2015 Society of Chemical Industry C1 [Eiden, Amanda L.; Kaufman, Phillip E.; Oi, Faith] Univ Florida, Dept Entomol & Nematol, 1881 Nat Area Dr, Gainesville, FL 32611 USA. [Allan, Sandra A.] ARS, USDA, CMAVE, Gainesville, FL USA. RP Eiden, AL (reprint author), Univ Florida, Dept Entomol & Nematol, 1881 Nat Area Dr, Gainesville, FL 32611 USA. EM amandalynneiden@gmail.com OI Kaufman, Phillip/0000-0001-6159-8358 FU US Department of Agriculture Southern Region Integrated Pest Management program [FLA-ENY-2009-34103-19907, 2009-41530-05595] FX We thank Glenn Cawthorne (Ecto Services Inc., Henderson, NC) for providing the susceptible tick strain, and Emma Weeks, Salvador Gezan Lucas Carnohan, Lois Wood and the tick-contributing clientele and pest control operators. Funding was provided by the US Department of Agriculture Southern Region Integrated Pest Management program project, FLA-ENY-2009-34103-19907 and 2009-41530-05595. NR 13 TC 1 Z9 1 U1 3 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD JUL PY 2016 VL 72 IS 7 BP 1390 EP 1395 DI 10.1002/ps.4165 PG 6 WC Agronomy; Entomology SC Agriculture; Entomology GA DR0MT UT WOS:000379602300016 PM 26449370 ER PT J AU Keathley, CP Stelinski, LL Lapointe, SL AF Keathley, Craig P. Stelinski, Lukasz L. Lapointe, Stephen L. TI Investigating dormant-season application of pheromone in citrus to control overwintering and spring populations of Phyllocnistis citrella (Lepidoptera: Gracillariidae) SO PEST MANAGEMENT SCIENCE LA English DT Article DE citrus; leafminer; sex pheromone; mating disruption; preventive management ID CODLING MOTH LEPIDOPTERA; FEMALE SEX-PHEROMONE; MATING DISRUPTION; LEAFMINER LARVAE; FIELD-EVALUATION; STAINTON; TORTRICIDAE; MANAGEMENT; ABUNDANCE; FLORIDA AB BACKGROUND: The leafminer, Phyllocnistis citrella Stainton, reproduces on leaf flush during winter. Deployment of pheromone during winter could suppress moth populations in spring and summer more than a spring application alone. We tested the primary pheromone component of P. citrella, (Z,Z,E)-7,11,13-hexadecatrienal, released gradually over several months from elastomeric dispensers in a citrus grove in 6.4 ha main plots in winter and/or 3.2 ha subplots in spring (834 mg triene ha(-1)) and evaluated moth catch and leaf mining. RESULTS: After winter treatment, dispensers provided >85% disruption of male moth catch in traps for 37 weeks, and after spring treatment they provided >92% disruption for 26 weeks, but there was only a 12% reduction in leaf infestation in spring. Two applications were no better than only a single application in spring. Disruption of moth catch was weaker in treated plots where traps were placed high (3.1 m) rather than low (1.6 m) in the tree canopy. CONCLUSION: Dispensers provided effective and persistent disruption of male catch in pheromone-baited monitoring traps but were minimally effective in reducing leaf infestation by P. citrella. Winter application of pheromone did not reduce leaf mining in spring compared with spring application alone. Tops of trees may have provided a refuge formating. Published 2015. This article is a U. S. Government work and is in the public domain in the USA. C1 [Keathley, Craig P.] EI du Pont Nemours & Co, Stine Haskell Res Ctr, 1090 Elkton Rd, Newark, DE 19711 USA. [Keathley, Craig P.; Lapointe, Stephen L.] ARS, USDA, US Hort Res Lab, Ft Pierce, FL USA. [Stelinski, Lukasz L.] Univ Florida, Ctr Agr Res & Educ, IFAS, Lake Alfred, FL 33850 USA. RP Keathley, CP (reprint author), EI du Pont Nemours & Co, Stine Haskell Res Ctr, 1090 Elkton Rd, Newark, DE 19711 USA. EM craig.keathley@dupont.com FU Citrus Research and Development Foundation FX We thank Larry Markle, Denis Willett, Josh MacNaught, Ryland Mermer, Jermaine Thomas, Rafael Forero, Paul Robbins, Rocco Alessandro, Ian Jackson, Bo Holladay and Scott Holladay for technical assistance. This research was funded in part by the Citrus Research and Development Foundation. Mention of a trademark or proprietary product is solely for the purpose of providing specific information and does not constitute a guarantee or warranty of the product by the US Department of Agriculture and does not imply its approval to the exclusion of other products that may also be suitable. NR 29 TC 0 Z9 0 U1 7 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD JUL PY 2016 VL 72 IS 7 BP 1405 EP 1410 DI 10.1002/ps.4167 PG 6 WC Agronomy; Entomology SC Agriculture; Entomology GA DR0MT UT WOS:000379602300018 PM 26446918 ER PT J AU Blake, VC Birkett, C Matthews, DE Hane, DL Bradbury, P Jannink, JL AF Blake, Victoria C. Birkett, Clay Matthews, David E. Hane, David L. Bradbury, Peter Jannink, Jean-Luc TI The Triticeae Toolbox: Combining Phenotype and Genotype Data to Advance Small-Grains Breeding SO PLANT GENOME LA English DT Article ID GENOMIC SELECTION; BIG DATA; RESOURCE; ASSOCIATION; COMMUNITY; FUTURE AB The Triticeae Toolbox (http://triticeaetoolbox.org; T3) is the database schema enabling plant breeders and researchers to combine, visualize, and interrogate the wealth of phenotype and genotype data generated by the Triticeae Coordinated Agricultural Project (TCAP). T3 enables users to define specific data sets for download in formats compatible with the external tools TASSEL, Flapjack, and R; or to use by software residing on the T3 server for operations such as Genome Wide Association and Genomic Prediction. New T3 tools to assist plant breeders include a Selection Index Generator, analytical tools to compare phenotype trials using common or user-defined indices, and a histogram generator for nursery reports, with applications using the Android OS, and a Field Plot Layout Designer in development. Researchers using T3 will soon enjoy the ability to design training sets, define core germplasm sets, and perform multivariate analysis. An increased collaboration with GrainGenes and integration with the small grains reference sequence resources will place T3 in a pivotal role for on-the-fly data analysis, with instant access to the knowledge databases for wheat and barley. T3 software is available under the GNU General Public License and is freely downloadable. C1 [Blake, Victoria C.; Hane, David L.] ARS, USDA, Western Reg Res Ctr, 800 Buchanan St, Albany, CA USA. [Birkett, Clay; Matthews, David E.; Bradbury, Peter; Jannink, Jean-Luc] ARS, USDA, Robert Holley Ctr, Tower Rd, Ithaca, NY 14853 USA. RP Jannink, JL (reprint author), ARS, USDA, Robert Holley Ctr, Tower Rd, Ithaca, NY 14853 USA. EM JeanLuc.Jannink@ars.usda.gov FU Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2011-68002-30029]; USDA-ARS [5325-21000-014-00] FX This research was supported by the Agriculture and Food Research Initiative Competitive Grant No. 2011-68002-30029 from the USDA National Institute of Food and Agriculture. Maintenance and further development of T3 by GrainGenes is supported by USDA-ARS project 5325-21000-014-00, "An Integrated Database and Bioinformatics Resource for Small Grains." Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. Upon request, all novel materials described in this publication will be made available in a timely manner for noncommercial research purposes, subject to the requisite permission from any third-party owners of all or parts of the material. Obtaining any permissions will be the responsibility of the requestor. NR 32 TC 0 Z9 0 U1 8 U2 8 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD JUL PY 2016 VL 9 IS 2 DI 10.3835/plantgenome2014.12.0099 PG 10 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA DQ7EC UT WOS:000379368700001 ER PT J AU Chaffin, AS Huang, YF Smith, S Bekele, WA Babiker, E Gnanesh, BN Foresman, BJ Blanchard, SG Jay, JJ Reid, RW Wight, CP Chao, SM Oliver, R Islamovic, E Kolb, FL McCartney, C Fetch, JWM Beattie, AD Bjornstad, A Bonman, JM Langdon, T Howarth, CJ Brouwer, CR Jellen, EN Klos, KE Poland, JA Hsieh, TF Brown, R Jackson, E Schlueter, JA Tinker, NA AF Chaffin, Ashley S. Huang, Yung-Fen Smith, Scott Bekele, Wubishet A. Babiker, Ebrahiem Gnanesh, Belaghihalli N. Foresman, Bradley J. Blanchard, Steven G. Jay, Jeremy J. Reid, Robert W. Wight, Charlene P. Chao, Shiaoman Oliver, Rebekah Islamovic, Emir Kolb, Frederic L. McCartney, Curt Fetch, Jennifer W. Mitchell Beattie, Aaron D. Bjornstad, Asmund Bonman, J. Michael Langdon, Tim Howarth, Catherine J. Brouwer, Cory R. Jellen, Eric N. Klos, Kathy Esvelt Poland, Jesse A. Hsieh, Tzung-Fu Brown, Ryan Jackson, Eric Schlueter, Jessica A. Tinker, Nicholas A. TI A Consensus Map in Cultivated Hexaploid Oat Reveals Conserved Grass Synteny with Substantial Subgenome Rearrangement SO PLANT GENOME LA English DT Article ID QUANTITATIVE TRAIT LOCI; AVENA-SATIVA L.; LINKAGE MAP; CROWN RUST; RESISTANCE; MARKERS; GENOME; CHROMOSOMES; BARLEY AB Hexaploid oat (Avena sativa L., 2n = 6x = 42) is a member of the Poaceae family and has a large genome (similar to 12.5 Gb) containing 21 chromosome pairs from three ancestral genomes. Physical rearrangements among parental genomes have hindered the development of linkage maps in this species. The objective of this work was to develop a single high-density consensus linkage map that is representative of the majority of commonly grown oat varieties. Data from a cDNA-derived single-nucleotide polymorphism (SNP) array and genotyping-by-sequencing (GBS) were collected from the progeny of 12 biparental recombinant inbred line populations derived from 19 parents representing oat germplasm cultivated primarily in North America. Linkage groups from all mapping populations were compared to identify 21 clusters of conserved collinearity. Linkage groups within each cluster were then merged into 21 consensus chromosomes, generating a framework consensus map of 7202 markers spanning 2843 cM. An additional 9678 markers were placed on this map with a lower degree of certainty. Assignment to physical chromosomes with high confidence was made for nine chromosomes. Comparison of homeologous regions among oat chromosomes and matches to orthologous regions of rice (Oryza sativa L.) reveal that the hexaploid oat genome has been highly rearranged relative to its ancestral diploid genomes as a result of frequent translocations among chromosomes. Heterogeneous chromosome rearrangements among populations were also evident, probably accounting for the failure of some linkage groups to match the consensus. This work contributes to a further understanding of the organization and evolution of hexaploid grass genomes. C1 [Chaffin, Ashley S.; Blanchard, Steven G.; Jay, Jeremy J.; Reid, Robert W.; Brouwer, Cory R.; Schlueter, Jessica A.] Univ N Carolina, Dept Bioinformat & Gen, 9201 Univ City Blvd, Charlotte, NC 28223 USA. [Huang, Yung-Fen; Bekele, Wubishet A.; Wight, Charlene P.; Tinker, Nicholas A.] Agr & Agri Food Canada, Ottawa Res & Dev Ctr, CE Farm, 960 Carling Ave,KW Neatby Bldg, Ottawa, ON K1A 0C6, Canada. [Huang, Yung-Fen] Natl Taiwan Univ, Dept Agron, 1,Sect 4,Roosevelt Rd, Taipei 106, Taiwan. [Smith, Scott; Hsieh, Tzung-Fu] N Carolina State Univ, Dept Plant & Microbial Biol, 600 Laureate Way, Raleigh, NC 27695 USA. [Hsieh, Tzung-Fu] N Carolina State Univ, Plants Human Hlth Inst, Kannapolis, NC 28081 USA. [Babiker, Ebrahiem; Islamovic, Emir; Bonman, J. Michael; Klos, Kathy Esvelt] USDA ARS, Small Grains & Potato Germplasm Res Unit, 1691 S 2700 W, Aberdeen, ID 83210 USA. [Gnanesh, Belaghihalli N.] Univ Manitoba, Dept Plant Sci, 66 Dafoe Rd, Winnipeg, MB R3T 2N2, Canada. [Gnanesh, Belaghihalli N.; McCartney, Curt] Agr & Agri Food Canada, 101 Route 100, Morden, MB R6M 1Y5, Canada. [Foresman, Bradley J.; Kolb, Frederic L.] Univ Illinois, Dept Crop Sci, 1102 South Goodwin Ave, Urbana, IL 61801 USA. [Blanchard, Steven G.; Jay, Jeremy J.; Reid, Robert W.; Brouwer, Cory R.] Univ N Carolina, Bioinformat Serv Div, 150 N Res Campus Dr, Kannapolis, NC 28081 USA. [Chao, Shiaoman] USDA ARS, Cereal Crops Res, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. [Oliver, Rebekah] N Dakota State Univ, Dept Plant Sci, POB 6050, Fargo, ND 58108 USA. [Islamovic, Emir] BASF Plant Sci LP, 26 Davis Dr, Res Triangle Pk, NC 27709 USA. [Fetch, Jennifer W. Mitchell] Agr & Agri Food Canada, 2701 Grand Valley Rd, Brandon, MB R7A 5Y3, Canada. [Beattie, Aaron D.] Univ Saskatchewan, Dept Plant Sci, 51 Campus Dr, Saskatoon, SK S7N 5A8, Canada. [Bjornstad, Asmund] Norwegian Univ Life Sci, Dept Plant & Environm Sci, POB 5003, N-1432 As, Norway. [Langdon, Tim; Howarth, Catherine J.] Aberystwyth Univ, Inst Biol Environm & Rural Sci, Aberystwyth SY23 3EE, Dyfed, Wales. [Jellen, Eric N.] Brigham Young Univ, Dept Plant & Wildlife Sci, 5009 LSB, Provo, UT 84602 USA. [Poland, Jesse A.] Kansas State Univ, Dept Plant Pathol, Wheat Genet Resource Ctr, 4024 Throckmorton Plant Sci Ctr, Manhattan, KS 66506 USA. [Poland, Jesse A.] Kansas State Univ, Dept Agron, 4024 Throckmorton Plant Sci Ctr, Manhattan, KS 66506 USA. [Brown, Ryan] United States Patent & Trademark Off, 400 Dulany St, Alexandria, VA 22314 USA. [Jackson, Eric] Gen Mills Crop Sci, 1990 Kimball Ave, Manhattan, KS 66506 USA. RP Schlueter, JA (reprint author), Univ N Carolina, Dept Bioinformat & Gen, 9201 Univ City Blvd, Charlotte, NC 28223 USA.; Tinker, NA (reprint author), Agr & Agri Food Canada, Ottawa Res & Dev Ctr, CE Farm, 960 Carling Ave,KW Neatby Bldg, Ottawa, ON K1A 0C6, Canada. EM jschluet@uncc.edu; nick.tinker@agr.gc.ca OI Poland, Jesse/0000-0002-7856-1399 FU General Mills; North American Millers' Association; Prairie Oat Growers' Association of Canada; USDA-NIFA; Agriculture and Agri-food Canada; Plant Pathways Elucidation Project; National Science Foundation [1444575] FX ASC, Y-FH, and SS contributed equally as first authors; JAS and NAT contributed equally as senior corresponding authors. The authors acknowledge the generous support for this project provided to the Collaborative Oat Research Enterprise by General Mills, the North American Millers' Association, the Prairie Oat Growers' Association of Canada, USDA-NIFA, and Agriculture and Agri-food Canada. We are also grateful to the students and many sponsors of the Plant Pathways Elucidation Project (http://p2ep.org, accessed 9 Mar. 2016). We are indebted to many others, too numerous to mention, who provided excellent advice and technical support to the authors. Financial support to JAS was provided by National Science Foundation Award #1444575. NR 36 TC 7 Z9 7 U1 7 U2 9 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD JUL PY 2016 VL 9 IS 2 DI 10.3835/plantgenome2015.10.0102 PG 21 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA DQ7EC UT WOS:000379368700023 ER PT J AU Chakrabarti, M Dinkins, RD Hunt, AG AF Chakrabarti, Manohar Dinkins, Randy D. Hunt, Arthur G. TI De novo Transcriptome Assembly and Dynamic Spatial Gene Expression Analysis in Red Clover SO PLANT GENOME LA English DT Article ID TRIFOLIUM-PRATENSE L.; ARABIDOPSIS-THALIANA; TARGETED MUTAGENESIS; PROVIDES INSIGHT; DOWN-REGULATION; RAPD MARKERS; SSR MARKERS; GENOME; ANNOTATION; FLAVONOIDS AB Red clover (Trifolium pratense L.) is a cool-season forage legume grown throughout the northeastern United States and is the most widely planted forage legume after alfalfa (Medicago sativa L.). Red clover provides high-value feed to the livestock because of high protein content and easy digestibility. To date, genomic resources for red clover are scarce. In the current study, a de novo transcriptome assembly of red clover was constructed representing different tissue types. The draft assembly consists of 37,565 contigs with N50 and average contig length of 1707 and 1262 bp, respectively. A comparative study with three other legume species displayed a high degree of sequence conservation between red clover and other legumes. The assembled transcriptome was annotated to allow identification of desirable genes. In particular, a genome-wide identification of red clover transcripts encoding putative transcription factors was performed. A comparative gene expression analysis between different tissue types was performed using the assembled transcriptome as the reference, which revealed dynamic gene expression patterns across different tissue types and also identified spatially dynamic gene coexpression clusters. Genes representing tissue-enriched clusters were subjected to gene ontology (GO) enrichment analysis to identify over-represented functional groups. Identification of these tissue-enriched gene coexpression clusters can help in future research focusing on developmental studies across tissues or in biotechnological improvement of red clover. C1 [Chakrabarti, Manohar; Dinkins, Randy D.; Hunt, Arthur G.] Univ Kentucky, Dep Plant & Soil Sci, Lexington, KY 40546 USA. [Dinkins, Randy D.] ARS, USDA, Forage Anim Prod Res Unit, Lexington, KY 40546 USA. RP Dinkins, RD; Hunt, AG (reprint author), Univ Kentucky, Dep Plant & Soil Sci, Lexington, KY 40546 USA.; Dinkins, RD (reprint author), ARS, USDA, Forage Anim Prod Res Unit, Lexington, KY 40546 USA. EM randy.dinkins@ars.usda.gov; aghunt00@uky.edu OI Dinkins, Randy/0000-0002-2127-273X FU USDA-ARS-Forage-Animal Production Research Unit [58-6440-3-005] FX We would like to thank Troy Bass for his excellent support in maintaining red clover plants in the greenhouse and Carol Von Lanken for excellent technical assistance. This work was funded through a specific cooperative agreement with USDA-ARS-Forage-Animal Production Research Unit (58-6440-3-005). NR 66 TC 0 Z9 0 U1 6 U2 10 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD JUL PY 2016 VL 9 IS 2 DI 10.3835/plantgenome2015.06.0048 PG 12 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA DQ7EC UT WOS:000379368700004 ER PT J AU Klos, KE Huang, YF Bekele, WA Obert, DE Babiker, E Beattie, AD Bjornstad, A Bonman, JM Carson, ML Chao, S Gnanesh, BN Griffiths, I Harrison, SA Howarth, CJ Hu, GS Ibrahim, A Islamovic, E Jackson, EW Jannink, JL Kolb, FL McMullen, MS Fetch, JM Murphy, JP Ohm, HW Rines, HW Rossnagel, BG Schlueter, JA Sorrells, ME Wight, CP Yan, WK Tinker, NA AF Klos, Kathy Esvelt Huang, Yung-Fen Bekele, Wubishet A. Obert, Don E. Babiker, Ebrahiem Beattie, Aaron D. Bjornstad, Asmund Bonman, J. Michael Carson, Martin L. Chao, Shiaoman Gnanesh, Belaghihalli N. Griffiths, Irene Harrison, Stephen A. Howarth, Catherine J. Hu, Gongshe Ibrahim, Amir Islamovic, Emir Jackson, Eric W. Jannink, Jean-Luc Kolb, Frederic L. McMullen, Michael S. Fetch, Jennifer Mitchell Murphy, J. Paul Ohm, Herbert W. Rines, Howard W. Rossnagel, Brian G. Schlueter, Jessica A. Sorrells, Mark E. Wight, Charlene P. Yan, Weikai Tinker, Nicholas A. TI Population Genomics Related to Adaptation in Elite Oat Germplasm SO PLANT GENOME LA English DT Article ID BETA-GLUCAN CONCENTRATION; LINKAGE DISEQUILIBRIUM; WIDE ASSOCIATION; WORLDWIDE ORIGIN; FLOWERING TIME; HEADING DATE; WHEAT; MAP; VERNALIZATION; MARKERS AB Six hundred thirty five oat (Avena sativa L.) lines and 4561 single-nucleotide polymorphism (SNP) loci were used to evaluate population structure, linkage disequilibrium (LD), and genotype-phenotype association with heading date. The first five principal components (PCs) accounted for 25.3% of genetic variation. Neither the eigenvalues of the first 25 PCs nor the cross-validation errors from K = 1 to 20 model-based analyses suggested a structured population. However, the PC and K = 2 model-based analyses supported clustering of lines on spring oat vs. southern United States origin, accounting for 16% of genetic variation (p < 0.0001). Single-locus F-statistic (F-ST) in the highest 1% of the distribution suggested linkage groups that may be differentiated between the two population subgroups. Population structure and kinship-corrected LD of r(2) = 0.10 was observed at an average pairwise distance of 0.44 cM (0.71 and 2.64 cM within spring and southern oat, respectively). On most linkage groups LD decay was slower within southern lines than within the spring lines. A notable exception was found on linkage group Mrg28, where LD decay was substantially slower in the spring subpopulation. It is speculated that this may be caused by a heterogeneous translocation event on this chromosome. Association with heading date was most consistent across location-years on linkage groups Mrg02, Mrg12, Mrg13, and Mrg24. C1 [Klos, Kathy Esvelt; Bekele, Wubishet A.; Bonman, J. Michael; Hu, Gongshe] USDA ARS, Small Grains & Potato Germplasm Res Unit, 1691 South 2700 West, Aberdeen, ID 83210 USA. [Huang, Yung-Fen] Natl Taiwan Univ, Dept Agron, Taipei 10764, Taiwan. [Bekele, Wubishet A.; Wight, Charlene P.; Yan, Weikai; Tinker, Nicholas A.] AAFC, ECORC, Ottawa, ON K1A 0C6, Canada. [Beattie, Aaron D.; Rossnagel, Brian G.] Univ Saskatchewan, Ctr Crop Dev, 51 Campus Dr, Saskatoon, SK S7N 5A8, Canada. [Obert, Don E.] Limagrain Cereal Seeds, 4846 East 450 North, Lafayette, IN 47905 USA. [Bjornstad, Asmund] Norwegian Univ Life Sci, Dept Plant & Environm Sci, POB 5003, N-1432 As, Norway. [Carson, Martin L.] USDA ARS, Cereal Dis Lab, 1551 Lindig Ave, St Paul, MN 55108 USA. [Chao, Shiaoman] USDA ARS, Cereal Crops Res Unit, Fargo, ND 58102 USA. [Gnanesh, Belaghihalli N.; Fetch, Jennifer Mitchell] AAFC, Cereal Res Ctr, 101 Route 100, Morden, MB R6M 1Y5, Canada. [Griffiths, Irene; Howarth, Catherine J.] Aberystwyth Univ, Inst Biol Environm & Rural Sci, Aberystwyth SY23 3EE, Dyfed, Wales. [Harrison, Stephen A.] Louisiana State Univ, 221 MB Sturgis, Baton Rouge, LA 70803 USA. [Ibrahim, Amir] Texas A&M Univ, Dept Crop & Soil Sci, 430C Heep Ctr, College Stn, TX 77843 USA. [Islamovic, Emir] BASF, 26 Davis Dr, Res Triangle Pk, NC 27709 USA. [Jackson, Eric W.] Gen Mills Inc, Manhattan, KS 66502 USA. [Jannink, Jean-Luc] USDA ARS, Plant Soil & Nutr Res, Robert W Holley Ctr, Tower Rd, Ithaca, NY 14853 USA. [Kolb, Frederic L.] Univ Illinois, Dept Crop Sci, AE 120 Turner Hall,1102 S Goodwin, Urbana, IL 61801 USA. [McMullen, Michael S.] N Dakota State Univ, Plant Sci, POB 6050, Fargo, ND 58108 USA. [Murphy, J. Paul] N Carolina State Univ, Dept Crop Sci, Campus Box 7620, Raleigh, NC 27695 USA. [Ohm, Herbert W.] Purdue Univ, Dept Agron, 915 West State St, W Lafayette, IN 47907 USA. [Rines, Howard W.] Univ Minnesota, Dept Agron & Plant Genet, 1991 Upper Buford Circle, St Paul, MN 55108 USA. [Schlueter, Jessica A.] Univ N Carolina, Coll Comp & Informat, 9201 Univ City Blvd, Charlotte, NC 28223 USA. [Sorrells, Mark E.] Cornell Univ, Plant Breeding & Genet, 240 Emerson Hall, Ithaca, NY 14853 USA. RP Klos, KE (reprint author), USDA ARS, Small Grains & Potato Germplasm Res Unit, 1691 South 2700 West, Aberdeen, ID 83210 USA. EM Kathy.klos@ars.usda.gov FU USDA; Agriculture and Agri-Food Canada; North American Millers' Association; Prairie Oat Growers' Association FX Funding for this work was provided by the USDA, Agriculture and Agri-Food Canada, the North American Millers' Association, and the Prairie Oat Growers' Association. We thank Robert Campbell, Rachel Redman, and Irene Shackelford for their technical assistance. We also thank Drs. Kyle Gardner, Rick Jellen, Gerard Lazo, Joe Lutz, Jeff Maughan, Curt McCartney, Rebekah Oliver, and Deon Stuthman (deceased) for participating in useful discussion. NR 47 TC 2 Z9 2 U1 8 U2 8 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD JUL PY 2016 VL 9 IS 2 DI 10.3835/plantgenome2015.10.0103 PG 12 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA DQ7EC UT WOS:000379368700024 ER PT J AU Mahoney, LL Sargent, DJ Abebe-Akele, F Wood, DJ Ward, JA Bassil, NV Hancock, JF Folta, KM Davis, TM AF Mahoney, Lise L. Sargent, Daniel J. Abebe-Akele, F. Wood, Dave J. Ward, Judson A. Bassil, Nahla V. Hancock, James F. Folta, Kevin M. Davis, Thomas M. TI A High-Density Linkage Map of the Ancestral Diploid Strawberry, Fragaria iinumae, Constructed with Single Nucleotide Polymorphism Markers from the IStraw90 Array and Genotyping by Sequencing SO PLANT GENOME LA English DT Article ID ROSACEAE; GENOMICS; VESCA AB Fragaria iinumae Makino is recognized as an ancestor of the octoploid strawberry species, which includes the cultivated strawberry, Fragaria xananassa Duchesne ex Rozier. Here we report the construction of the first high-density linkage map for F. iinumae. The F. iinumae linkage map (Fii map) is based on two high-throughput techniques of single nucleotide polymorphism (SNP) genotyping: the IStraw90 Array (hereafter "Array"), and genotyping by sequencing (GBS). The F-2 generation mapping population was derived by selfing F. iinumae hybrid F1D, the product of a cross between two divergent F. iinumae accessions collected from Hokkaido, Japan. The Fii map consists of seven linkage groups (LGs) and has an overall length of 451.7 cM as defined by 496 loci populated by 4173 markers: 3280 from the Array and 893 from GBS. Comparisons with two versions of the Fragaria vesca ssp. vesca L. 'Hawaii 4' pseudo-chromosome (PC) assemblies reveal substantial conservation of synteny and colinearity, yet identified differences that point to possible genomic divergences between F. iinumae and F. vesca, and/or to F. vesca genomic assembly errors. The Fii map provides a basis for anchoring a F. iinumae genome assembly as a prerequisite for constructing a second diploid reference genome for Fragaria. C1 [Mahoney, Lise L.; Sargent, Daniel J.; Davis, Thomas M.] Univ New Hampshire, Dept Biol Sci, Rudman Hall, Durham, NH 03824 USA. [Sargent, Daniel J.] Fdn Edmund Mach, Res & Innovat Ctr, I-38010 San Michele All Adige, Trento, Italy. [Sargent, Daniel J.] Driscolls Genet Ltd, New Rd, East Malling ME19 6BJ, Kent, England. [Abebe-Akele, F.] Univ New Hampshire, Hubbard Ctr Genome Studies, 444 Gregg Hall, Durham, NH 03824 USA. [Ward, Judson A.] Driscolls, Mol Biol Dept, 151 Silliman Rd, Watsonville, CA 95076 USA. [Bassil, Nahla V.] ARS, USDA, Natl Clonal Germplasm Repository, 33447 Peoria Rd, Corvallis, OR 97333 USA. [Hancock, James F.] Michigan State Univ, Dept Hort, 342 Plant & Soil Sci Bldg, E Lansing, MI 48824 USA. [Folta, Kevin M.] Univ Florida, Dept Hort Sci, Gainesville, FL 32611 USA. RP Mahoney, LL (reprint author), Univ New Hampshire, Dept Biol Sci, Rudman Hall, Durham, NH 03824 USA. EM mahoney@unh.edu FU USDA-CSREES NRI Plant Genome Grant [2008-35300-04411]; New Hampshire Agricultural Experiment Station (NHAES); USDA National Inst. of Food and Agriculture Hatch Project [0193637]; Autonomous Province of Trento grant office; Specialty Crop Research Initiative Competitive Grant of the USDA's National Inst. of Food and Agriculture [2009-51181-05808]; USDA-ARS CRIS [2072-21000-044-00D] FX This work was supported in part by the USDA-CSREES NRI Plant Genome Grant 2008-35300-04411 to TMD. Partial funding was provided by the New Hampshire Agricultural Experiment Station (NHAES). This paper is NHAES Scientific Contribution no. 2619. This work was also supported by the USDA National Inst. of Food and Agriculture Hatch Project 0193637 grant to TMD. Fragaria molecular genetics at Fondazione Edmund Mach is sponsored by funding from the Autonomous Province of Trento grant office (to DJS). Funding for "RosBREED: Enabling Marker-Assisted Breeding in Rosaceae," which furnished the genotyping for the initial set of 24 plants, was provided by the Specialty Crop Research Initiative Competitive Grant 2009-51181-05808 of the USDA's National Inst. of Food and Agriculture (to TMD and NVB). Financial support was also provided by the USDA-ARS CRIS Project 2072-21000-044-00D to NVB. We thank Kelley Thomas at the Hubbard Center for Genome Studies and Yilong Yang for their assistance with GBS data handling and analysis. NR 32 TC 0 Z9 0 U1 11 U2 12 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD JUL PY 2016 VL 9 IS 2 DI 10.3835/plantgenome2015.08.0071 PG 14 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA DQ7EC UT WOS:000379368700007 ER PT J AU Mamidi, S Miklas, PN Trapp, J Felicetti, E Grimwood, J Schmutz, J Lee, R McClean, PE AF Mamidi, Sujan Miklas, Phillip N. Trapp, Jennifer Felicetti, Erin Grimwood, Jane Schmutz, Jeremy Lee, Rian McClean, Phillip E. TI Sequence-Based Introgression Mapping Identifies Candidate White Mold Tolerance Genes in Common Bean SO PLANT GENOME LA English DT Article ID RECEPTOR-LIKE PROTEINS; DISEASE-RESISTANCE; SCLEROTINIA-SCLEROTIORUM; PHYSIOLOGICAL RESISTANCE; QTL; GENOME; ARABIDOPSIS; SUSCEPTIBILITY; SUPERFAMILY; INHERITANCE AB White mold, caused by the necrotrophic fungus Sclerotinia sclerotiorum (Lib.) de Bary, is a major disease of common bean (Phaseolus vulgaris L.). WM7.1 and WM8.3 are two quantitative trait loci (QTL) with major effects on tolerance to the pathogen. Advanced backcross populations segregating individually for either of the two QTL, and a recombinant inbred (RI) population segregating for both QTL were used to fine map and confirm the genetic location of the QTL. The QTL intervals were physically mapped using the reference common bean genome sequence, and the physical intervals for each QTL were further confirmed by sequence-based introgression mapping. Using whole-genome sequence data from susceptible and tolerant DNA pools, introgressed regions were identified as those with significantly higher numbers of single-nucleotide polymorphisms (SNPs) relative to the whole genome. By combining the QTL and SNP data, WM7.1 was located to a 660-kb region that contained 41 gene models on the proximal end of chromosome Pv07, while the WM8.3 introgression was narrowed to a 1.36-Mb region containing 70 gene models. The most polymorphic candidate gene in the WM7.1 region encodes a BEACH-domain protein associated with apoptosis. Within the WM8.3 interval, a receptor-like protein with the potential to recognize pathogen effectors was the most polymorphic gene. The use of gene and sequence-based mapping identified two candidate genes whose putative functions are consistent with the current model of Sclerotinia pathogenicity. C1 [Mamidi, Sujan; Lee, Rian; McClean, Phillip E.] North Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Miklas, Phillip N.; Trapp, Jennifer; Felicetti, Erin] ARS, USDA, Grain Legume Genet & Physiol Res Unit, 24106 N Bunn Rd, Prosser, WA 99350 USA. [Grimwood, Jane; Schmutz, Jeremy] HudsonAlpha Inst Biotechnol, Huntsville, AL 35806 USA. [McClean, Phillip E.] North Dakota State Univ, Genom & Bioinformat Program, Fargo, ND 58108 USA. RP McClean, PE (reprint author), North Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA.; McClean, PE (reprint author), North Dakota State Univ, Genom & Bioinformat Program, Fargo, ND 58108 USA. EM phillip.mcclean@ndsu.edu OI mamidi, sujan/0000-0002-3837-6121 FU National Sclerotinia Initiative FX The authors would like to thank Justin Faris, USDA-ARS, Fargo, ND, for a critical review of the manuscript. The authors would also like to acknowledge the financial support to this project from the National Sclerotinia Initiative (http://www.ars.usda.gov/Research/docs.htm?docid=20122). NR 53 TC 0 Z9 0 U1 5 U2 7 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD JUL PY 2016 VL 9 IS 2 DI 10.3835/plantgenome2015.09.0092 PG 11 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA DQ7EC UT WOS:000379368700021 ER PT J AU Migicovsky, Z Gardner, KM Money, D Sawler, J Bloom, JS Moffett, P Chao, CT Schwaninger, H Fazio, G Zhong, GY Myles, S AF Migicovsky, Zoe Gardner, Kyle M. Money, Daniel Sawler, Jason Bloom, Joshua S. Moffett, Peter Chao, C. Thomas Schwaninger, Heidi Fazio, Gennaro Zhong, Gan-Yuan Myles, Sean TI Genome to Phenome Mapping in Apple Using Historical Data SO PLANT GENOME LA English DT Article ID X DOMESTICA BORKH.; ASSISTED SEEDLING SELECTION; NAC TRANSCRIPTION FACTORS; GENETIC ARCHITECTURE; ARABIDOPSIS-THALIANA; ETHYLENE PRODUCTION; NATURAL VARIATION; BREEDING PROGRAM; FRUIT-QUALITY; ASSOCIATION AB Apple (Malus X. domestica Borkh.) is one of the world's most valuable fruit crops. Its large size and long juvenile phase make it a particularly promising candidate for marker-assisted selection (MAS). However, advances in MAS in apple have been limited by a lack of phenotype and genotype data from sufficiently large samples. To establish genotype-phenotype relationships and advance MAS in apple, we extracted over 24,000 phenotype scores from the USDA-Germplasm Resources Information Network (GRIN) database and linked them with over 8000 single nucleotide polymorphisms (SNPs) from 689 apple accessions from the USDA apple germplasm collection clonally preserved in Geneva, NY. We find significant genetic differentiation between Old World and New World cultivars and demonstrate that the genetic structure of the domesticated apple also reflects the time required for ripening. A genome-wide association study (GWAS) of 36 phenotypes confirms the association between fruit color and the MYB1 locus, and we also report a novel association between the transcription factor, NAC18.1, and harvest date and fruit firmness. We demonstrate that harvest time and fruit size can be predicted with relatively high accuracies (r > 0.46) using genomic prediction. Rapid decay of linkage disequilibrium (LD) in apples means millions of SNPs may be required for well-powered GWAS. However, rapid LD decay also promises to enable extremely high resolution mapping of causal variants, which holds great potential for advancing MAS. C1 [Migicovsky, Zoe; Gardner, Kyle M.; Money, Daniel; Sawler, Jason; Myles, Sean] Dalhousie Univ, Fac Agr, Dept Plant & Anim Sci, Truro, NS, Canada. [Sawler, Jason] Anandia Labs, 2259 Lower Mall, Vancouver, BC, Canada. [Bloom, Joshua S.] Univ Calif Los Angeles, Howard Hughes Med Inst, Dept Human Genet, Los Angeles, CA 90024 USA. [Moffett, Peter] Univ Sherbrooke, Dept Biol, Sherbrooke, PQ, Canada. [Chao, C. Thomas; Schwaninger, Heidi; Fazio, Gennaro; Zhong, Gan-Yuan] ARS, USDA, Plant Genet Resources Unit, Geneva, NY USA. RP Myles, S (reprint author), Dalhousie Univ, Fac Agr, Dept Plant & Anim Sci, Truro, NS, Canada.; Zhong, GY (reprint author), ARS, USDA, Plant Genet Resources Unit, Geneva, NY USA. EM GanYuan.Zhong@ars.usda.gov; sean.myles@dal.ca OI Migicovsky, Zoe/0000-0002-3931-1258 FU Canada Research Chairs program; National Sciences and Engineering Research Council of Canada; Genome Canada; Dalhousie University FX This project was enabled through the Cooperative Agreement (58-1910-02-029FN) entitled "Characterization of genomic diversity and evolutionary origins of the USDA-ARS Malus germplasm collection" between the USDA-ARS and Dalhousie University. We thank the people who manage and maintain the USDA apple germplasm collection in Geneva, NY, and also those who made the phenotype data from the apple collection available via the USDA-GRIN database. We would like to acknowledge Angela Baldo, Chris Richards, and Gayle Volk for their contributions to initial discussions of data processing. We would also like to acknowledge Daryl Somers for useful input. This article was written, in part, thanks to funding from the Canada Research Chairs program, the National Sciences and Engineering Research Council of Canada, and Genome Canada. Z.M. was supported in part by a Killam Predoctoral Scholarship from Dalhousie University. NR 83 TC 0 Z9 0 U1 12 U2 18 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD JUL PY 2016 VL 9 IS 2 DI 10.3835/plantgenome2015.11.0113 PG 15 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA DQ7EC UT WOS:000379368700029 ER PT J AU Punnuri, SM Wallace, JG Knoll, JE Hyma, KE Mitchell, SE Buckler, ES Varshney, RK Singh, BP AF Punnuri, Somashekhar M. Wallace, Jason G. Knoll, Joseph E. Hyma, Katie E. Mitchell, Sharon E. Buckler, Edward S. Varshney, Rajeev K. Singh, Bharat P. TI Development of a High-Density Linkage Map and Tagging Leaf Spot Resistance in Pearl Millet Using Genotyping-by-Sequencing Markers SO PLANT GENOME LA English DT Article ID QUANTITATIVE TRAIT LOCI; GRASS PENNISETUM-PURPUREUM; L. R. BR.; STOVER YIELD; GENETIC-MAP; PYRICULARIA-GRISEA; DROUGHT TOLERANCE; DETERMINING GRAIN; REPEAT MARKERS; GLAUCUM AB Pearl millet [Pennisetum glaucum (L.) R. Br; also Cenchrus americanus (L.) Morrone] is an important crop throughout the world but better genomic resources for this species are needed to facilitate crop improvement. Genome mapping studies are a prerequisite for tagging agronomically important traits. Genotyping-by-sequencing (GBS) markers can be used to build high-density linkage maps, even in species lacking a reference genome. A recombinant inbred line (RIL) mapping population was developed from a cross between the lines 'Tift 99D(2)B(1)' and 'Tift 454'. DNA from 186 RILs, the parents, and the F-1 was used for 96-plex ApeKI GBS library development, which was further used for sequencing. The sequencing results showed that the average number of good reads per individual was 2.2 million, the pass filter rate was 88%, and the CV was 43%. High-quality GBS markers were developed with stringent filtering on sequence data from 179 RILs. The reference genetic map developed using 150 RILs contained 16,650 single-nucleotide polymorphisms (SNPs) and 333,567 sequence tags spread across all seven chromosomes. The overall average density of SNP markers was 23.23 SNP/cM in the final map and 1.66 unique linkage bins per cM covering a total genetic distance of 716.7 cM. The linkage map was further validated for its utility by using it in mapping quantitative trait loci (QTLs) for flowering time and resistance to Pyricularia leaf spot [Pyricularia grisea (Cke.) Sacc.]. This map is the densest yet reported for this crop and will be a valuable resource for the pearl millet community. C1 [Punnuri, Somashekhar M.; Singh, Bharat P.] Ft Valley State Univ, Agr Res Stn, 1005 State Univ Dr, Ft Valley, GA 31030 USA. [Wallace, Jason G.] Univ Georgia, Dept Crop & Soil Sci, Athens, GA 30602 USA. [Wallace, Jason G.] Cornell Univ, Inst Genom Divers, Ithaca, NY 14853 USA. [Knoll, Joseph E.] ARS, USDA, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. [Hyma, Katie E.; Mitchell, Sharon E.] Cornell Univ, Inst Biotechnol, Genom Divers Facil, Ithaca, NY 14853 USA. [Buckler, Edward S.] Cornell Univ, USDA, ARS, Inst Genom Divers,Dep Plant Breeding & Genet, Ithaca, NY 14853 USA. [Varshney, Rajeev K.] Int Crops Res Inst Semi Arid Trop, Hyderabad 502324, Telangana, India. RP Punnuri, SM (reprint author), Ft Valley State Univ, Agr Res Stn, 1005 State Univ Dr, Ft Valley, GA 31030 USA. EM punnuris@fvsu.edu RI Punnuri, Somashekhar/D-9195-2016 OI Punnuri, Somashekhar/0000-0002-4542-4160 FU USDA-National Institute of Food and Agriculture [GEOX-2008-02595]; NSF [IOS-1238014]; University of Georgia; USDA-ARS FX We thank Eli Rodgers-Melnick for part of the R code for rippling LGs, the Pearl Millet Genome Sequencing Consortium for use of prepublication contigs and the Genomic Diversity Facility (Cornell University) for helpful advice on GBS analysis. We thank Ms. Chrisdon B. Bonner for helping us to improve the quality of the manuscript. We are also grateful for funding support received from the capacity building project by USDA-National Institute of Food and Agriculture Grant # GEOX-2008-02595, NSF Grant IOS-1238014, the University of Georgia, and the USDA-ARS. The authors declare that they have no competing interests related to the contents of this manuscript. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 88 TC 0 Z9 0 U1 4 U2 7 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD JUL PY 2016 VL 9 IS 2 DI 10.3835/plantgenome2015.10.0106 PG 13 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA DQ7EC UT WOS:000379368700026 ER PT J AU Tang, Y Liu, XL Wang, JB Li, M Wang, QS Tian, F Su, ZB Pan, YC Liu, D Lipka, AE Buckler, ES Zhang, ZW AF Tang, You Liu, Xiaolei Wang, Jiabo Li, Meng Wang, Qishan Tian, Feng Su, Zhongbin Pan, Yuchun Liu, Di Lipka, Alexander E. Buckler, Edward S. Zhang, Zhiwu TI GAPIT Version 2: An Enhanced Integrated Tool for Genomic Association and Prediction SO PLANT GENOME LA English DT Article ID LINEAR MIXED MODELS; WIDE ASSOCIATION; POPULATION-STRUCTURE; SELECTION AB Most human diseases and agriculturally important traits are complex. Dissecting their genetic architecture requires continued development of innovative and powerful statistical methods. Corresponding advances in computing tools are critical to efficiently use these statistical innovations and to enhance and accelerate biomedical and agricultural research and applications. The genome association and prediction integrated tool (GAPIT) was first released in 2012 and became widely used for genome-wide association studies (GWAS) and genomic prediction. The GAPIT implemented computationally efficient statistical methods, including the compressed mixed linear model (CMLM) and genomic prediction by using genomic best linear unbiased prediction (gBLUP). New state-of-the-art statistical methods have now been implemented in a new, enhanced version of GAPIT. These methods include factored spectrally transformed linear mixed models (FaST-LMM), enriched CMLM (ECMLM), FaST-LMM-Select, and settlement of mixed linear models under progressively exclusive relationship (SUPER). The genomic prediction methods implemented in this new release of the GAPIT include gBLUP based on CMLM, ECMLM, and SUPER. Additionally, the GAPIT was updated to improve its existing output display features and to add new data display and evaluation functions, including new graphing options and capabilities, phenotype simulation, power analysis, and cross-validation. These enhancements make the GAPIT a valuable resource for determining appropriate experimental designs and performing GWAS and genomic prediction. The enhanced R-based GAPIT software package uses state-of-the-art methods to conduct GWAS and genomic prediction. The GAPIT also provides new functions for developing experimental designs and creating publication-ready tabular summaries and graphs to improve the efficiency and application of genomic research. C1 [Tang, You; Su, Zhongbin] Northeast Agr Univ, Coll Elect & Informat, Harbin, Peoples R China. [Liu, Xiaolei] Huazhong Agr Univ, Minist Educ, Key Lab Agr Anim Genet Breeding & Reprod, Wuhan, Peoples R China. [Liu, Xiaolei] Huazhong Agr Univ, Coll Anim Sci & Technol, Wuhan, Peoples R China. [Liu, Xiaolei; Buckler, Edward S.] Cornell Univ, Inst Genom Divers, Ithaca, NY 14853 USA. [Wang, Jiabo; Zhang, Zhiwu] North East Agr Univ, Dept Anim Sci & Technol, Harbin, Peoples R China. [Wang, Jiabo; Liu, Di] Heilongjiang Acad Agr Sci, Inst Anim Husb, Harbin, Peoples R China. [Li, Meng] Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China. [Wang, Qishan; Pan, Yuchun] Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai, Peoples R China. [Tian, Feng] China Agr Univ, Natl Maize Improvement Ctr China, Beijing, Peoples R China. [Lipka, Alexander E.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Buckler, Edward S.] ARS, USDA, Ithaca, NY 14853 USA. [Zhang, Zhiwu] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. RP Zhang, ZW (reprint author), North East Agr Univ, Dept Anim Sci & Technol, Harbin, Peoples R China.; Zhang, ZW (reprint author), Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. EM Zhiwu.Zhang@WSU.edu FU NSF [0922493, 1238014]; USDA-ARS; Agricultural Research Center at Washington State University; College of Agricultural, Human, and Natural Resource Sciences; Washington Grain Commission [126593]; National Natural Science Foundation of China [31301748]; China Postdoctoral Science Foundation [2014M551607] FX This study was supported by NSF (0922493 and 1238014), USDA-ARS, an Emerging Research Issues Internal Competitive Grant from the Agricultural Research Center at Washington State University, College of Agricultural, Human, and Natural Resource Sciences, the Endowment and Research Project (No. 126593) from the Washington Grain Commission, the National Natural Science Foundation of China (Grant no. 31301748), and the China Postdoctoral Science Foundation (Grant no. 2014M551607). The authors thank Linda R. Klein for copyediting the manuscript. NR 27 TC 0 Z9 0 U1 17 U2 28 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD JUL PY 2016 VL 9 IS 2 DI 10.3835/plantgenome2015.11.0120 PG 9 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA DQ7EC UT WOS:000379368700032 ER PT J AU Wolfe, MD Rabbi, IY Egesi, C Hamblin, M Kawuki, R Kulakow, P Lozano, R Del Carpio, DP Ramu, P Jannink, JL AF Wolfe, Marnin D. Rabbi, Ismail Y. Egesi, Chiedozie Hamblin, Martha Kawuki, Robert Kulakow, Peter Lozano, Roberto Del Carpio, Dunia Pino Ramu, Punna Jannink, Jean-Luc TI Genome-Wide Association and Prediction Reveals Genetic Architecture of Cassava Mosaic Disease Resistance and Prospects for Rapid Genetic Improvement SO PLANT GENOME LA English DT Article ID MANIHOT-ESCULENTA; MODEL APPROACH; SELECTION; GEMINIVIRUSES; REGRESSION; DEFENSE; PLANTS; AFRICA; WHEAT; POPULATIONS AB Cassava (Manihot esculenta Crantz) is a crucial, under-researched crop feeding millions worldwide, especially in Africa. Cassava mosaic disease (CMD) has plagued production in Africa for over a century. Biparental mapping studies suggest primarily a single major gene mediates resistance. To investigate this genetic architecture, we conducted the first genome-wide association mapping study in cassava with up to 6128 genotyping-by-sequenced African breeding lines and 42,113 reference genome-mapped single-nucleotide polymorphism (SNP) markers. We found a single region on chromosome 8 that accounts for 30 to 66% of genetic resistance in the African cassava germplasm. Thirteen additional regions with small effects were also identified. Further dissection of the major quantitative trait locus (QTL) on chromosome 8 revealed the presence of two possibly epistatic loci and/or multiple resistance alleles, which may account for the difference between moderate and strong disease resistances in the germplasm. Search of potential candidate genes in the major QTL region identified two peroxidases and one thioredoxin. Finally, we found genomic prediction accuracy of 0.53 to 0.58 suggesting that genomic selection (GS) will be effective both for improving resistance in breeding populations and identifying highly resistant clones as varieties. C1 [Wolfe, Marnin D.; Hamblin, Martha; Kawuki, Robert; Del Carpio, Dunia Pino; Ramu, Punna; Jannink, Jean-Luc] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14850 USA. [Rabbi, Ismail Y.; Kulakow, Peter] Int Inst Trop Agr, Ibadan, Oyo, Nigeria. [Egesi, Chiedozie] Natl Root Crops Res Inst, Umudike, Umuahia, Nigeria. [Kawuki, Robert] Natl Crops Resources Res Inst, Namulonge, Uganda. [Jannink, Jean-Luc] ARS, USDA, RW Holley Ctr Agr & Hlth, Ithaca, NY USA. RP Wolfe, MD (reprint author), Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14850 USA. EM wolfemd@gmail.com FU Bill & Melinda Gates Foundation; UKaid [1048542]; CGIAR Research Program on Roots, Tubers and Bananas FX We acknowledge the Bill & Melinda Gates Foundation and UKaid (Grant 1048542; http://www.gatesfoundation.org) and support from the CGIAR Research Program on Roots, Tubers and Bananas (http://www.rtb.cgiar.org). We give special thanks to A.G.O. Dixon for his development of many of the breeding lines and historical data we analyzed. Thanks also to A. I. Smith and technical teams at IITA, NRCRI, and NaCRRI for collection of phenotypic data and to A. Agbona, A. Ogbonna, E. Uba, and R. Mukisa for data curation. This work is dedicated to the memory of Martha Hamblin. NR 59 TC 0 Z9 0 U1 20 U2 23 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD JUL PY 2016 VL 9 IS 2 DI 10.3835/plantgenome2015.11.0118 PG 13 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA DQ7EC UT WOS:000379368700031 ER PT J AU Zhou, L Luo, LH Zuo, JF Yang, LF Zhang, L Guang, XM Niu, Y Jian, JB Geng, QC Liang, LP Song, QJ Dunwell, JM Wu, ZZ Wen, J Liu, YQ Zhang, YM AF Zhou, Ling Luo, Longhai Zuo, Jian-Fang Yang, Linfeng Zhang, Li Guang, Xuanmin Niu, Yuan Jian, Jianbo Geng, Qing-Chun Liang, Liping Song, Qijian Dunwell, Jim M. Wu, Zhenzhen Wen, Jia Liu, Yu-Qin Zhang, Yuan-Ming TI Identification and Validation of Candidate Genes Associated with Domesticated and Improved Traits in Soybean SO PLANT GENOME LA English DT Article ID MULTILOCUS GENOTYPE DATA; GLYCINE-MAX L.; RECOMBINANT INBRED LINES; MADS-BOX GENES; ARABIDOPSIS-THALIANA; FLOWERING-TIME; POPULATION-STRUCTURE; SEED SIZE; CIRCADIAN CLOCK; ABC TRANSPORTER AB Soybean, an important source of vegetable oils and proteins for humans, has undergone significant phenotypic changes during domestication and improvement. However, there is limited knowledge about genes related to these domesticated and improved traits, such as flowering time, seed development, alkaline-salt tolerance, and seed oil content (SOC). In this study, more than 106,000 single nucleotide polymorphisms (SNPs) were identified by restriction site associated DNA sequencing of 14 wild, 153 landrace, and 119 bred soybean accessions, and 198 candidate domestication regions (CDRs) were identified via multiple genetic diversity analyses. Of the 1489 candidate domestication genes (CDGs) within these CDRs, a total of 330 CDGs were related to the above four traits in the domestication, gene ontology (GO) enrichment, gene expression, and pathway analyses. Eighteen, 60, 66, and 10 of the 330 CDGs were significantly associated with the above four traits, respectively. Of 134 trait-associated CDGs, 29 overlapped with previous CDGs, 11 were consistent with candidate genes in previous trait association studies, and 66 were covered by the domesticated and improved quantitative trait loci or their adjacent regions, having six common CDGs, such as one functionally characterized gene Glyma15 g17480 (GmZTL3). Of the 68 seed size (SS) and SOC CDGs, 37 were further confirmed by gene expression analysis. In addition, eight genes were found to be related to artificial selection during modern breeding. Therefore, this study provides an integrated method for efficiently identifying CDGs and valuable information for domestication and genetic research. C1 [Zhou, Ling; Zuo, Jian-Fang; Zhang, Yuan-Ming] Huazhong Agr Univ, Coll Plant Sci & Technol, Stat Genom Lab, Wuhan 430070, Peoples R China. [Zhou, Ling] Jiangsu Acad Agr Sci, Inst Agrobiotechnol, Nanjing 210014, Jiangsu, Peoples R China. [Zhou, Ling; Zhang, Li; Niu, Yuan; Geng, Qing-Chun; Wen, Jia] Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Natl Ctr Soybean Improvement, Nanjing 210095, Jiangsu, Peoples R China. [Luo, Longhai; Yang, Linfeng; Guang, Xuanmin; Jian, Jianbo; Liang, Liping; Wu, Zhenzhen] BGI Shenzhen, Shenzhen 518083, Peoples R China. [Song, Qijian] ARS, Soybean Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. [Dunwell, Jim M.] Univ Reading, Sch Agr Policy & Dev, Reading RG6 6AR, Berks, England. [Liu, Yu-Qin] Linyi Acad Agr Sci, Crop Res Inst, Linyi 276012, Peoples R China. RP Zhang, YM (reprint author), Huazhong Agr Univ, Coll Plant Sci & Technol, Stat Genom Lab, Wuhan 430070, Peoples R China. EM soyzhang@mail.hzau.edu.cn OI Zhang, Yuan-Ming/0000-0003-2317-2190; Dunwell, Jim/0000-0003-2147-665X FU National Natural Science Foundation of China [31571268]; Fundamental Research Funds for the Central Universities [KYT201002]; Huazhong Agricultural University Scientific and Technological Self-innovation Foundation [2014RC020] FX This research was supported by the National Natural Science Foundation of China (31571268), Fundamental Research Funds for the Central Universities (KYT201002), and Huazhong Agricultural University Scientific and Technological Self-innovation Foundation (2014RC020). NR 133 TC 0 Z9 0 U1 7 U2 11 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD JUL PY 2016 VL 9 IS 2 DI 10.3835/plantgenome2015.09.0090 PG 17 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA DQ7EC UT WOS:000379368700019 ER PT J AU Nichols, MH Polyakov, VO Nearing, MA Hernandez, M AF Nichols, Mary H. Polyakov, Viktor O. Nearing, Mark A. Hernandez, Mariano TI Semiarid Watershed Response to Low-Tech Porous Rock Check Dams SO SOIL SCIENCE LA English DT Article DE Check dams; grade stabilization; sediment yield; runoff; channel morphology ID EROSION CONTROL; SEDIMENT YIELD; GULLY CONTROL; ARIZONA; SPAIN AB Rock check dams are used throughout the world to mitigate erosion problems on degraded lands. Many restoration efforts on rangelands in the southwestern United States incorporate such structures; however, their impact on watershed response and channel morphology is not well quantified. In 2008, 37 porous rock structures were built on two small (4.0 and 3.1 ha) instrumented watersheds on an alluvial fan at the base of the Santa Rita Mountains in southern Arizona. Thirty-five years of historical rainfall, runoff, and sediment data are available to compare with 7 years of data collected after check dam construction. In addition, postconstruction measurements of channel geometry and longitudinal channel profiles were compared with preconstruction measurements. The primary impact of the check dams was retention of channel sediment and reduction in channel gradient; however, response varied between the proximal watersheds, with 80% of the check dams on one of the watersheds filled to 100% of their capacity after seven runoff seasons. Precipitation runoff ratios changed after construction, but the change was not persistent after check dams filled to capacity. The contrasting watershed experiences lower sediment yields, and only 20% of the check dams on this watershed was filled to capacity. Within the watersheds, the mean gradient of the channel reach immediately upstream of the structures has been reduced by 35% (from 0.061 to 0.039) and 34% (from 0.071 to 0.047). C1 [Nichols, Mary H.; Polyakov, Viktor O.; Nearing, Mark A.; Hernandez, Mariano] USDA ARS, Southwest Watershed Res Ctr, 2000 E Allen Rd, Tucson, AZ 85719 USA. RP Nichols, MH (reprint author), USDA ARS, Southwest Watershed Res Ctr, 2000 E Allen Rd, Tucson, AZ 85719 USA. EM mary.nichols@ars.usda.gov NR 22 TC 1 Z9 1 U1 9 U2 14 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0038-075X EI 1538-9243 J9 SOIL SCI JI Soil Sci. PD JUL PY 2016 VL 181 IS 7 BP 275 EP 282 DI 10.1097/SS.0000000000000160 PG 8 WC Soil Science SC Agriculture GA DQ8CX UT WOS:000379437800002 ER PT J AU Abdelwahab, OMM Bingner, RL Milillo, F Gentile, F AF Abdelwahab, Ossama Mohamed Mahmoud Bingner, Ronald L. Milillo, Fabio Gentile, Francesco TI Evaluation of Alternative Management Practices With the AnnAGNPS Model in the Carapelle Watershed SO SOIL SCIENCE LA English DT Article DE AnnAGNPS model; soil erosion; sediment load; best management practices ID NONPOINT-SOURCE POLLUTION; TILL FARMING SYSTEMS; SOIL-EROSION; AGRICULTURAL WATERSHEDS; STRATEGIC TILLAGE; SEDIMENT YIELD; RUNOFF; FLOW; IMPLEMENTATION; SIMULATIONS AB The Annualized Agricultural Non-point Source (AnnAGNPS) model can be used to analyze the effects of management practices on sediment loads in agricultural watersheds. The study was performed in a 506 km(2) Mediterranean watershed located in Apulia, Southern Italy, planted with mostly winter wheat (83%) where runoff and sediment loads have been monitored at an in-stream gage. The AnnAGNPS model was used to predict runoff and sediment load without calibration during a 5-year period. On an annual scale, the model showed good prediction capability for runoff (R-2 = 0.8, NSE [Nash and Sutcliffe coefficient of efficiency] = 0.7) and satisfactory results for sediment load (NSE = 0.5, R-2 = 0.5). Based on the current conditions of the watershed as a baseline scenario, the effectiveness of alternative conservation practices applied within the watershed was also evaluated. No-tillage practices applied to the entire cropland area reduced soil erosion within fields by 44% and sediment yield from fields to streams by 20%. Reduced tillage decreased soil erosion, sediment yield, and sediment load at the gage location by 12%, 7%, and 4% respectively. Limiting the placement of alternative practices to the cropland sub-watersheds with the most erosion (Scenarios G and H) proved to be a promising and viable approach to sediment erosion reduction throughout the watershed. In this perspective, areas that produce the most sediment were identified and targeted for replacement of varying levels of cropland with forest, and consequently sediment loads were reduced from 5% to 97%. The effect of vegetated streams and riparian buffers as natural traps that can increase the in situ sediment deposition was also considered. Most, but not all of the scenarios discussed herein could realistically be implemented within the watershed, particularly if there are incentive policies. Even considering the intrinsic uncertainty of modeling results, evaluating these systems with the aid of AnnAGNPS serves as a means to provide reference information and allows watershed conservation planners to compare the impacts of different management scenarios with sustainable agriculture guidelines. C1 [Abdelwahab, Ossama Mohamed Mahmoud; Milillo, Fabio; Gentile, Francesco] Univ Bari, Dept Agr & Environm Sci, Bari, Italy. [Abdelwahab, Ossama Mohamed Mahmoud] Cairo Univ, Fac Agr, Dept Agr Engn, Cairo, Egypt. [Bingner, Ronald L.] ARS, USDA, Natl Sedimentat Lab, Watershed Phys Proc Res Unit, Oxford, MS USA. [Bingner, Ronald L.] ARS, USDA, Natl Sedimentat Lab, Water Qual & Ecol Res Unit, Oxford, MS USA. RP Abdelwahab, OMM (reprint author), Via Amendola 165-A, I-70121 Bari, Italy. EM osama.mahmoud@agr.cu.edu.eg RI feng, yongzhong/F-5090-2012 OI feng, yongzhong/0000-0002-5202-4368 NR 78 TC 2 Z9 2 U1 8 U2 11 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0038-075X EI 1538-9243 J9 SOIL SCI JI Soil Sci. PD JUL PY 2016 VL 181 IS 7 BP 293 EP 305 DI 10.1097/SS.0000000000000162 PG 13 WC Soil Science SC Agriculture GA DQ8CX UT WOS:000379437800004 ER PT J AU Nicklas, T Liu, Y Giovanni, M Jahns, L Tucker, K Laugero, K Bogle, M Chester, D AF Nicklas, Theresa Liu, Yan Giovanni, Maria Jahns, Lisa Tucker, Katherine Laugero, Kevin Bogle, Margaret Chester, Deirdra TI Association between barriers and facilitators to meeting the Dietary Guidelines for Americans and body weight status of caregiver-child dyads: the Healthy Eating and Lifestyle for Total Health Study SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE HEALTH; barriers; facilitators; Dietary Guidelines for Americans; BMI ID CORONARY-ARTERY-DISEASE; VEGETABLE CONSUMPTION; WHOLE-GRAIN; EPIDEMIOLOGIC EVIDENCE; FAMILY RESEMBLANCE; PERCEIVED BARRIERS; UNITED-STATES; RISK-FACTORS; FRUIT; ADHERENCE AB Background: Few Americans meet the Dietary Guidelines for Americans (DGA), yet a large percentage are overweight. Objective: The goal of this research was to examine the association between barriers and facilitators to meeting the DGA and weight in a multisite study. Design: This was a cross-sectional study in which 836 caregiver-child dyads among 3 race/ethnic groups completed the questionnaire across 6 sites participating in the HEALTH (Healthy Eating and Lifestyle for Total Health) Study. Barriers and facilitators to meeting the DGA were assessed by using a validated questionnaire developed specifically for the HEALTH Study. Heights and weights were measured. A series of bivariate regression analyses were conducted to examine the association between caregiver body mass index (BMI) or child BMI z score and the barriers and facilitators to meeting the DGA, adjusting for appropriate covariates in the models. Path analysis was used to examine the relation of caregiver and child barriers and facilitators to their relative BMIs. Results: In children, the BMI z score was associated positively (P < 0.001) with total barriers and negatively (P < 0.001) with total facilitators. In caregivers, BMI was associated positively only with total bathers (P < 0.0001). For each of the 8 MyPyramid components, bathers consistently were associated positively (P < 0.0001) and facilitators were associated negatively (P < 0.001) with BMI z score in children (with the exception of the fruit and physical activity components). For caregivers, most of the barriers to meeting recommendations for meats and beans; solid fats, alcohol, and added sugars; and physical activity components were associated positively (P < 0.01) with BMI. Path analyses show that caregiver facilitators were significantly related to children's facilitators to meeting DGA recommendations (standardized beta: 0.12; P < 0.001). Conclusion: To our knowledge, our findings are among the first to show associations between weight and barriers and/or facilitators to meeting the DGA in a national, large sample of caregiver child dyads. C1 [Nicklas, Theresa; Liu, Yan] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Giovanni, Maria] Calif State Univ Chico, Dept Nutr & Food Sci, Chico, CA 95929 USA. [Jahns, Lisa] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. [Tucker, Katherine] Univ Massachusetts, Dept Clin Lab & Nutr Sci, Lowell, MA 01852 USA. [Laugero, Kevin] Univ Calif Davis, USDA ARS, Western Human Nutr Res Ctr, Obes & Metab Res Unit, Davis, CA 95616 USA. [Bogle, Margaret] USDA ARS, Delta Obes Prevent Res Unit, Dallas, TX USA. [Chester, Deirdra] USDA ARS, Beltsville Human Nutr Res Ctr, Beltsville, MD USA. [Chester, Deirdra] USDA, Natl Inst Food & Agr, Inst Food Safety & Nutr, Washington, DC 20250 USA. RP Nicklas, T (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. EM tnicklas@bcm.edu FU USDA Agricultural Research Service (ARS) [58-3092-5-001]; Dairy Management Incorporated FX Supported by the USDA Agricultural Research Service (ARS) through the specific cooperative agreement 58-3092-5-001. Partial support was also received by Dairy Management Incorporated, which manages the National Dairy Council. NR 58 TC 0 Z9 0 U1 3 U2 3 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JUL PY 2016 VL 104 IS 1 BP 143 EP 154 DI 10.3945/ajcn.115.123372 PG 12 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DQ1RD UT WOS:000378977200019 PM 27194306 ER PT J AU Alzaman, NS Dawson-Hughes, B Nelson, J D'Alessio, D Pittas, AG AF Alzaman, Naweed S. Dawson-Hughes, Bess Nelson, Jason D'Alessio, David Pittas, Anastassios G. TI Vitamin D status of black and white Americans and changes in vitamin D metabolites after varied doses of vitamin D supplementation SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE vitamin D; free vitamin D; vitamin D-binding protein; clinical trial; African American; white; supplementation ID D-BINDING PROTEIN; NUTRITION EXAMINATION SURVEY; RANDOMIZED CONTROLLED-TRIAL; BONE-MINERAL DENSITY; 25-HYDROXYVITAMIN D; AFRICAN-AMERICANS; PARATHYROID-HORMONE; CALCIUM-ABSORPTION; RACIAL-DIFFERENCES; NATIONAL-HEALTH AB Background: Controversy exists over the disparate circulating 25-hydroxyvitamin D [25(OH)D] concentrations between black and white Americans. Objective: We sought to determine whether there are differences in total and directly measured free 25(OH)D concentrations between black and white American adults and how daily supplementation with cholecalciferol changes these concentrations. Design: Cross-sectional and longitudinal analyses were conducted with the use of data from 2 placebo-controlled, randomized trials at 2 academic medical centers in the United States: CaDDM (Calcium and Vitamin D in Type 2 Diabetes) and DDM2 (Vitamin D for Established Type 2 Diabetes). A total of 208 subjects with pre- or well-controlled diabetes with a mean age of 59.1 y and mean body mass index (BMI; in kg/m(2)) of 31.6 were randomly assigned to receive daily cholecalciferol supplementation at 1 of 2 doses (2000 or 4000 IU) or a matching placebo for 16 wk. We measured serum total 25(OH)D, vitamin D binding protein (DBP) by 2 different immunoassays (with the use of monoclonal or polyclonal antibodies), parathyroid hormone, and albumin. Free 25(OH)D concentration was directly measured and calculated. Results: Blacks had lower total 25(OH)D concentrations than whites [adjusted median: 20.3 ng/mL (95% CI: 16.2, 24.5 ng/mL) compared with 26.7 ng/mL (95% CI: 25.2, 28.1 ng/mL), respectively; P = 0.026)], and a higher proportion of blacks had total 25(OH)D concentrations <20 ng/mL (46% compared with 19%, respectively; P < 0.001). Directly measured free 25(OH)D concentrations were lower in blacks than in whites [adjusted median: 4.5 ng/mL (95% CI: 3.7, 5.4 ng/mL) compared with 5.7 ng/mL (95% CI: 5.4, 5.9 ng/mL), respectively; P = 0.044] and were strongly correlated with total 25(OH)D without an effect of race. DBP was lower in blacks when measured by the monoclonal but not the polyclonal antibody immunoassay. Cholecalciferol supplementation increased total and measured free 25(OH)D concentrations proportionally to the dose and without a difference between races. Conclusions: The relation between free and total 25(OH)D did not vary systematically by race in this multiracial population with pre- or well-controlled diabetes. The results need to be replicated in additional cohorts before concluding that the clinical assessment of vitamin D status in blacks and whites should follow a single standard. C1 [Alzaman, Naweed S.; Dawson-Hughes, Bess; Pittas, Anastassios G.] Tufts Med Ctr, Div Endocrinol Diabet & Metab, Boston, MA USA. [Nelson, Jason] Tufts Med Ctr, Predict Analyt & Comparat Effectiveness Ctr, Boston, MA USA. [Alzaman, Naweed S.] Taibah Univ, Coll Med, Dept Internal Med, Medina, Saudi Arabia. [Dawson-Hughes, Bess] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Bone Metab Lab, Boston, MA USA. [D'Alessio, David] Duke Univ, Med Ctr, Div Endocrinol, Durham, NC USA. RP Pittas, AG (reprint author), Tufts Med Ctr, Div Endocrinol Diabet & Metab, Boston, MA USA. EM apittas@tuftsmedicalcenter.org FU National Institute of Diabetes and Digestive and Kidney Disease [R01DK76092]; NIH Office of Dietary Supplements from the National Center for Research Resources [UL1 RR025752]; USDA [58-1950-4-401] FX Supported by research grant R01DK76092 (to AGP) from the National Institute of Diabetes and Digestive and Kidney Disease and the NIH Office of Dietary Supplements, UL1 RR025752 (to Tufts Medical Center) from the National Center for Research Resources, and USDA cooperative agreement 58-1950-4-401 (to BDH). NR 46 TC 3 Z9 3 U1 4 U2 7 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JUL PY 2016 VL 104 IS 1 BP 205 EP 214 DI 10.3945/ajcn.115.129478 PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DQ1RD UT WOS:000378977200026 PM 27194308 ER PT J AU Maggi, M Tourn, E Negri, P Szawarski, N Marconi, A Gallez, L Medici, S Ruffinengo, S Brasesco, C De Feudis, L Quintana, S Sammataro, D Eguaras, M AF Maggi, Matias Tourn, Elian Negri, Pedro Szawarski, Nicolas Marconi, Alfredo Gallez, Liliana Medici, Sandra Ruffinengo, Sergio Brasesco, Constanza De Feudis, Leonardo Quintana, Silvina Sammataro, Diana Eguaras, Martin TI A new formulation of oxalic acid for Varroa destructor control applied in Apis mellifera colonies in the presence of brood SO APIDOLOGIE LA English DT Article DE Varroa destructor; control; oxalic acid; bee brood; Argentina ID BEE COLONIES; FORMIC-ACID; RESISTANCE; HONEY; ARGENTINA; EFFICACY; ACARICIDES; RESIDUES AB An organic product based on oxalic acid was evaluated for use in Varroa control under spring/summer climatic conditions in Argentina. The formulation consists of four strips made of cellulose impregnated with a solution based on oxalid acid. Forty-eight beehives were used to assess the product efficacy. Residues of the product were also tested in honey, bees, and wax. Each trial had respective control groups without oxalic treatment. At the beginning of the experiment, four strips of the formulation were applied to the colonies belonging to the treated group. Falling mites were counted after 7, 14, 21, 28, 35, and 42 days. After the last count, the strips were removed and colonies received two flumethrin strips for 45 days. Falling mites were counted throughout this period. Average efficacy of the organic product was 93.1 % with low variability. This product is an organic treatment designed for Varroa control during brood presence and represents a good alternative to the synthetic treatments. C1 [Maggi, Matias; Negri, Pedro; Szawarski, Nicolas; Medici, Sandra; Brasesco, Constanza; De Feudis, Leonardo; Eguaras, Martin] Univ Nacl Mar del Plata, CIAS, Fac Ciencias Exactas & Nat, Funes 3350, RA-7600 Mar Del Plata, Argentina. [Maggi, Matias; Negri, Pedro; Medici, Sandra; Eguaras, Martin] Consejo Nacl Invest Cient & Tecn, Rivadavia1917,C1033AJ, Buenos Aires, DF, Argentina. [Tourn, Elian; Marconi, Alfredo] Univ Nacl Sur, Dept Agron, Buenos Aires, DF, Argentina. [Tourn, Elian; Marconi, Alfredo] EEA INTA Bordenave, Minist Agr Ganaderia & Pesca Nac, Buenos Aires, DF, Argentina. [Tourn, Elian; Marconi, Alfredo] Cooperat Trabajo Apicola Pampero Ltda, Buenos Aires, DF, Argentina. [Gallez, Liliana] Univ Nacl Sur, LabEA, Dept Agron, RA-8000 Bahia Blanca, Buenos Aires, Argentina. [Ruffinengo, Sergio] Univ Nacl Mar del Plata, Fac Ciencias Agr, Catedra Apicultura, RA-7620 Balcarce, Argentina. [Quintana, Silvina] Fares Taie Inst Anal, Lab Biol Mol, Mar Del Plata, Buenos Aires, Argentina. [Sammataro, Diana] ARS, USDA, Carl Hayden Honey Bee Res Ctr, Tucson, AZ USA. RP Maggi, M (reprint author), Univ Nacl Mar del Plata, CIAS, Fac Ciencias Exactas & Nat, Funes 3350, RA-7600 Mar Del Plata, Argentina. EM biomaggi@gmail.com FU ANPCyT, PICT FX The authors would like to thank the Universidad Nacional de Mar del Plata (UNMDP) and Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET). This research was supported by a grant of ANPCyT, PICT 2011 to M. M. NR 33 TC 1 Z9 1 U1 11 U2 17 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 0044-8435 EI 1297-9678 J9 APIDOLOGIE JI Apidologie PD JUL PY 2016 VL 47 IS 4 BP 596 EP 605 DI 10.1007/s13592-015-0405-7 PG 10 WC Entomology SC Entomology GA DQ1ZR UT WOS:000379000400012 ER PT J AU Islam, N Nagy, A Garrett, WM Shelton, D Cooper, B Nou, XW AF Islam, Nazrul Nagy, Attila Garrett, Wesley M. Shelton, Dan Cooper, Bret Nou, Xiangwu TI Different Cellular Origins and Functions of Extracellular Proteins from Escherichia coli O157:H7 and O104:H4 as Determined by Comparative Proteomic Analysis SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID ENTERICA SEROVAR TYPHIMURIUM; PERIPLASMIC-LEAKY MUTANTS; MASS-SPECTROMETRY DATA; IDENTIFICATION TECHNOLOGY; VIRULENCE FACTORS; OUTBREAK STRAIN; GROUND-BEEF; SEQUENCE; O157-H7; O104/H4 AB Extracellular proteins play important roles in bacterial interactions with the environmental matrices. In this study, we examined the extracellular proteins from Escherichia coli O157:H7 and O104:H4 by tandem mass spectrometry. We identified 500 and 859 proteins from the growth media of E. coli O157:H7 and O104:H4, respectively, including 371 proteins common to both strains. Among proteins that were considered specific to E. coli O157:H7 or present at higher relative abundances in O157:H7 medium, most (57 of 65) had secretion signal sequences in their encoding genes. Noticeably, the proteins included locus of enterocyte effacement (LEE) virulence factors, proteins required for peptidyl-lipoprotein accumulation, and proteins involved in iron scavenging. In contrast, a much smaller proportion of proteins (37 of 150) that were considered specific to O104:H4 or presented at higher relative abundances in O104:H4 medium had signals targeting them for secretion. These proteins included Shiga toxin 2 subunit B and O104:H4 signature proteins, including AAF/1 major fimbrial subunit and serine protease autotransporters. Most of the abundant proteins from the growth medium of E. coli O104:H4 were annotated as having functions in the cytoplasm. We provide evidence that the extensive presence of cytoplasmic proteins in E. coli O104:H4 growth medium was due to biological processes independent of cell lysis, indicating alternative mechanisms for this potent pathogen releasing cytoplasmic contents into the growth milieu, which could play a role in interaction with the environmental matrices, such as pathogenesis and biofilm formation. IMPORTANCE In this study, we compared the extracellular proteins from two of the most prominent foodborne pathogenic E. coli organisms that have caused severe outbreaks in the United States and in Europe. E. coli O157:H7 is a well-studied Shiga toxigenic foodborne pathogen of the enterohemorrhagic pathotype that has caused numerous outbreaks associated with various contaminated foods worldwide. E. coli O104:H4 is a newly emerged Shiga toxigenic foodborne pathogen of the enteroaggregative pathotype that gained notoriety for causing one of the most deadly foodborne outbreaks in Europe in 2011. Comparison of proteins in the growth medium revealed significant differences in the compositions of the extracellular proteins for these two pathogens. These differences may provide valuable information regarding the cellular responses of these pathogens to their environment, including cell survival and pathogenesis. C1 [Islam, Nazrul; Nagy, Attila; Shelton, Dan; Nou, Xiangwu] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. [Garrett, Wesley M.] ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD 20705 USA. [Cooper, Bret] ARS, Soybean Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. [Islam, Nazrul] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Nagy, Attila] NIAID, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA. RP Nou, XW (reprint author), ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA.; Cooper, B (reprint author), ARS, Soybean Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. EM bret.cooper@ars.usda.gov; xiangwu.nou@ars.usda.gov FU USDA-ARS; University of Maryland, College Park, MD FX This research was funded by USDA-ARS and a specific cooperative agreement between USDA-ARS and the University of Maryland, College Park, MD. NR 54 TC 1 Z9 1 U1 8 U2 8 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JUL PY 2016 VL 82 IS 14 BP 4371 EP 4378 DI 10.1128/AEM.00977-16 PG 8 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DQ6WB UT WOS:000379345100031 PM 27208096 ER PT J AU Skyba, O Cullen, D Douglas, CJ Mansfield, SD AF Skyba, Oleksandr Cullen, Dan Douglas, Carl J. Mansfield, Shawn D. TI Gene Expression Patterns of Wood Decay Fungi Postia placenta and Phanerochaete chrysosporium Are Influenced by Wood Substrate Composition during Degradation SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID CELLOBIOSE DEHYDROGENASE; CELLULOSE DEPOLYMERIZATION; POPULUS-TRICHOCARPA; PODOSPORA-ANSERINA; TRANSCRIPTOME; ENZYMES; HYDROLYSIS; GENOME; POLYSACCHARIDES; LIGNINOLYSIS AB Identification of the specific genes and enzymes involved in the fungal degradation of lignocellulosic biomass derived from feedstocks with various compositions is essential to the development of improved bioenergy processes. In order to elucidate the effect of substrate composition on gene expression in wood-rotting fungi, we employed microarrays based on the annotated genomes of the brown- and white-rot fungi, Rhodonia placenta (formerly Postia placenta) and Phanerochaete chrysosporium, respectively. We monitored the expression of genes involved in the enzymatic deconstruction of the cell walls of three 4-year-old Populus trichocarpa (poplar) trees of genotypes with distinct cell wall chemistries, selected from a population of several hundred trees grown in a common garden. The woody substrates were incubated with wood decay fungi for 10, 20, and 30 days. An analysis of transcript abundance in all pairwise comparisons highlighted 64 and 84 differentially expressed genes (> 2-fold, P < 0.05) in P. chrysosporium and P. placenta, respectively. Cross-fungal comparisons also revealed an array of highly differentially expressed genes (> 4-fold, P < 0.01) across different substrates and time points. These results clearly demonstrate that gene expression profiles of P. chrysosporium and P. placenta are influenced by wood substrate composition and the duration of incubation. Many of the significantly expressed genes encode "proteins of unknown function," and determining their role in lignocellulose degradation presents opportunities and challenges for future research. IMPORTANCE This study describes the variation in expression patterns of two wood-degrading fungi (brown-and white-rot fungi) during colonization and incubation on three different naturally occurring poplar substrates of differing chemical compositions, over time. The results clearly show that the two fungi respond differentially to their substrates and that several known and, more interestingly, currently unknown genes are highly misregulated in response to various substrate compositions. These findings highlight the need to characterize several unknown proteins for catalytic function but also as potential candidate proteins to improve the efficiency of enzymatic cocktails to degrade lignocellulosic substrates in industrial applications, such as in a biochemically based bioenergy platform. C1 [Skyba, Oleksandr; Mansfield, Shawn D.] Univ British Columbia, Dept Wood Sci, Vancouver, BC, Canada. [Cullen, Dan] US Forest Serv, USDA, Forest Prod Lab, Madison, WI USA. [Douglas, Carl J.] Univ British Columbia, Dept Bot, Vancouver, BC, Canada. RP Mansfield, SD (reprint author), Univ British Columbia, Dept Wood Sci, Vancouver, BC, Canada. EM shawn.mansfield@ubc.ca FU Genome British Columbia Applied Genomics Innovation Program [103BIO] FX This work was supported by the Genome British Columbia Applied Genomics Innovation Program (Project #103BIO) held by C.J.D and S.D.M. NR 44 TC 2 Z9 2 U1 15 U2 28 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JUL PY 2016 VL 82 IS 14 BP 4387 EP 4400 DI 10.1128/AEM.00134-16 PG 14 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DQ6WB UT WOS:000379345100033 PM 27208101 ER PT J AU Chang, CL Goodman, CL Ringbauer, J Geib, SM Stanley, D AF Chang, Chiou Ling Goodman, Cynthia L. Ringbauer, Joseph Geib, Scott M. Stanley, David TI LARVAL X-RAY IRRADIATION INFLUENCES PROTEIN EXPRESSION IN PUPAE OF THE ORIENTAL FRUIT FLY, BACTROCERA DORSALIS SO ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY LA English DT Article DE radiation; chitin; oriental fruit fly; gene expression ID LINE BCIRL-HZAM1 CELLS; DROSOPHILA-MELANOGASTER; GENE-EXPRESSION; DIPTERA; STRESS; IDENTIFICATION; TEPHRITIDAE; TOLERANCE; DIET AB The sterile insect technique (SIT) was developed to eradicate the new world screwworm from the southern United States and Mexico, and became a component of many area-wide integrated pest management programs, particularly useful in managing tephritid fruit flies. SIT is based on the idea of rearing and sterilizing male pests, originally by ionizing radiation, and then releasing into field, where they compete for and mate with wild females. Mating with sterile males leads to reduced fecundity to lower pest populations. There are concerns with the use and distribution of radioisotopes for SIT programs, which have led to developing X-ray irradiation protocols to sterilize insects. We considered the possibility that X-ray irradiation exerts sublethal impacts aside form sterilizing insects. Such effects may not be directly observable, which led us to the hypothesis that X-ray irradiation in one life stage creates alterations in biological fitness and protein expression in the subsequent stage. We tested our hypothesis by irradiating larvae of Bactrocera dorsalis. There are two major points. One, exposing larvae to X-ray treatments led to reduced adult emergence, fecundity, fertility, and flight capacity from the corresponding pupae and emerged adults. Two, the X-ray treatments led to substantial expression changes in 27 pupal proteins. We assorted the 67 spots representing these proteins into three groups, metabolism, development, and structure. Our interpretation is these X-ray induced changes in biological performance and protein expression indicate their adult counterparts may be disabled in their abilities to successfully compete for and mate wild females in native habitats. (C) 2016 Wiley Periodicals, Inc. C1 [Chang, Chiou Ling; Geib, Scott M.] USDA ARS, Daniel K Inouye US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. [Goodman, Cynthia L.; Ringbauer, Joseph; Stanley, David] USDA ARS, Biol Control Insects Res Lab, Columbia, MO 65205 USA. RP Chang, CL (reprint author), USDA ARS, Daniel K Inouye US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. EM stella.chang@ars.usda.gov NR 34 TC 0 Z9 0 U1 9 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0739-4462 EI 1520-6327 J9 ARCH INSECT BIOCHEM JI Arch. Insect Biochem. Physiol. PD JUL PY 2016 VL 92 IS 3 BP 192 EP 209 DI 10.1002/arch.21330 PG 18 WC Biochemistry & Molecular Biology; Entomology; Physiology SC Biochemistry & Molecular Biology; Entomology; Physiology GA DQ1CE UT WOS:000378937800003 PM 27079560 ER PT J AU Li, MX Sun, XM Cai, HF Sun, YJ Plath, M Li, CJ Lan, XY Lei, CZ Lin, FP Bai, YY Chen, H AF Li, Mingxun Sun, Xiaomei Cai, Hanfang Sun, Yujia Plath, Martin Li, Congjun Lan, Xianyong Lei, Chuzhao Lin, Fengpeng Bai, Yueyu Chen, Hong TI Long non-coding RNA ADNCR suppresses adipogenic differentiation by targeting miR-204 SO BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS LA English DT Article DE Long noncoding RNAs; Ribo-Zero RNA-Seq; Adipogenesis; ADNCR; ceRNA ID COMPETING ENDOGENOUS RNA; ADIPOCYTE DIFFERENTIATION; C/EBP-ALPHA; PPAR-GAMMA; EXPRESSION; CANCER; LNCRNA; SIRT1; TRANSCRIPTS; SPECIFICITY AB Adipogenesis is a complex and precisely orchestrated process mediated by a network of adipogenic regulatory factors. Several studies have highlighted the relevance of lncRNAs in adipocyte differentiation, but the precise molecular mechanism has largely remained elusive. In the present study, we performed Ribo-Zero RNA-Seq to investigate both the poly(A) + and poly(A)-lncRNAs of in vitro cultured bovine preadipocytes and differentiated adipocytes. A stringent set of 2882 lncRNAs was finally identified. A comparison of the lncRNAs expression profiles revealed that 16 lncRNAs are differentially expressed during adipocyte differentiation. We focused on the most downregulated lncRNA, which we named adipocyte differentiation-associated long noncoding RNA (ADNCR). Mechanistically, ADNCR inhibited adipocyte differentiation by functioning as a competing endogenous RNA (ceRNA) for miR-204, thereby augmenting the expression of the miR-204 target gene, SIRT1, which is known to inhibit adipocyte differentiation and adipogenic gene expression by docking with NCoR and SMART to repress PPAR gamma activity. Our data not only provide a valuable genomic resource for the identification of lncRNAs with functional roles in adipocyte differentiation but also reveal new insights into understanding the mechanisms of adipogenic differentiation. (C) 2016 Elsevier B.V. All rights reserved. C1 [Li, Mingxun; Sun, Xiaomei; Cai, Hanfang; Sun, Yujia; Plath, Martin; Lan, Xianyong; Lei, Chuzhao; Chen, Hong] Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China. [Li, Congjun] ARS, USDA, Anim Genom & Improvement Lab, Beltsville, MD USA. [Lin, Fengpeng] Bur Anim Husb Biyang Cty, Biyang 463700, Henan, Peoples R China. [Bai, Yueyu] Anim Hlth Supervis Henan Prov, Zhengzhou 450008, Henan, Peoples R China. RP Bai, YY; Chen, H (reprint author), Northwest A&F Univ, Coll Anim Sci & Technol, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China. EM postbai@sohu.com; chenhong1212@263.net FU Program of National Beef Cattle and Yak Industrial Technology System [CARS-38]; National Natural Science Foundation of China [31272408]; Science and Technology Co-ordinator Innovative Engineering Projects of Shaanxi Province [2015KTCL02-08, 2014KTZB02-02-02-02]; National Development and Reform Commission [2014-2573] FX This study was supported by the Program of National Beef Cattle and Yak Industrial Technology System (no. CARS-38), the National Natural Science Foundation of China (no. 31272408), the Science and Technology Co-ordinator Innovative Engineering Projects of Shaanxi Province (2015KTCL02-08, 2014KTZB02-02-02-02), and the Bio-breeding Capacity-building and Industry-specific Projects from the National Development and Reform Commission (No. 2014-2573). Project of breeding and application of Pinan Cattle. NR 49 TC 5 Z9 5 U1 10 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1874-9399 EI 0006-3002 J9 BBA-GENE REGUL MECH JI Biochim. Biophys. Acta-Gene Regul. Mech. PD JUL PY 2016 VL 1859 IS 7 BP 871 EP 882 DI 10.1016/j.bbagrm.2016.05.003 PG 12 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA DQ1KI UT WOS:000378959500005 PM 27156885 ER PT J AU Selhub, J Rosenberg, IH AF Selhub, Jacob Rosenberg, Irwin H. TI Excessive folic acid intake and relation to adverse health outcome SO BIOCHIMIE LA English DT Review DE Folic acid; Folate; Vitamin B12; Homocysteine; Methylmalonic acid ID PAIR DELETION POLYMORPHISM; KILLER-CELL CYTOTOXICITY; NEURAL-TUBE DEFECTS; DIHYDROFOLATE-REDUCTASE; VITAMIN-B-12 STATUS; UNITED-STATES; COGNITIVE IMPAIRMENT; TOTAL HOMOCYSTEINE; OLDER AMERICANS; FOLATE LEVELS AB The recent increase in the intake of folic acid by the general public through fortified foods and supplements, has raised safety concern based on early reports of adverse health outcome in elderly with low B12 status who took high doses of folic acid. These safety concerns are contrary to the 2015 WHO statement that "high folic acid intake has not reliably been shown to be associated with negative healeffects". In the folic acid post-fortification era, we have shown that in elderly participants in NHANES 1999-2002, high plasma folate level is associated with exacerbation of both clinical (anemia and cognitive impairment) and biochemical (high MMA and high Hcy plasma levels) signs of vitamin B12 deficiency. Adverse clinical outcomes in association with high folate intake were also seen among elderly with low plasma B12 levels from the Framingham Original Cohort and in a study from Australia which combined three elderly cohorts. Relation between high folate and adverse biochemical outcomes were also seen in the Sacramento Area Latino Study on Aging (High Hcy, high MMA and lower TC2) and at an outpatient clinic at Yale University where high folate is associated with higher MMA in the elderly but not in the young. Potential detrimental effects of high folic acid intake may not be limited to the elderly nor to those with B12 deficiency. A study from India linked maternal high RBC folate to increased insulin resistance in offspring. Our study suggested that excessive folic acid intake is associated with lower natural killer cells activity in elderly women. In a recent study we found that the risk for unilateral retinoblastoma in offspring is 4 fold higher in women that are homozygotes for the 19 bp deletion in the DHFR gene and took folic acid supplement during pregnancy. In the elderly this polymorphism is associated with lower memory and executive scores, both being significantly worse in those with high plasma folate. These and other data strongly imply that excessive intake of folic acid is not always safe in certain populations of different age and ethnical/genetic background. (C) 2016 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved. C1 [Selhub, Jacob; Rosenberg, Irwin H.] Tufts Univ, Jean Mayer USDA, Human Nutr Ctr Aging, Boston, MA 02111 USA. RP Selhub, J; Rosenberg, IH (reprint author), Tufts Univ, Jean Mayer USDA, Human Nutr Ctr Aging, Boston, MA 02111 USA. EM jacob.selhub@tufts.edu; irwin.rosenberg@tufts.edu NR 50 TC 4 Z9 4 U1 10 U2 18 PU ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER PI PARIS PA 23 RUE LINOIS, 75724 PARIS, FRANCE SN 0300-9084 EI 1638-6183 J9 BIOCHIMIE JI Biochimie PD JUL PY 2016 VL 126 SI SI BP 71 EP 78 DI 10.1016/j.biochi.2016.04.010 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA DQ1LW UT WOS:000378963500012 PM 27131640 ER PT J AU Balzli, C Lager, K Vincent, A Gauger, P Brockmeier, S Miller, L Richt, JA Ma, WJ Suarez, D Swayne, DE AF Balzli, Charles Lager, Kelly Vincent, Amy Gauger, Phillip Brockmeier, Susan Miller, Laura Richt, Juergen A. Ma, Wenjun Suarez, David Swayne, David E. TI Susceptibility of swine to H5 and H7 low pathogenic avian influenza viruses SO INFLUENZA AND OTHER RESPIRATORY VIRUSES LA English DT Article DE Avian influenza; H5; H7; infection; low pathogenic; swine susceptibility ID A VIRUSES; GENETIC REASSORTMENT; UNITED-STATES; PIGS; TRANSMISSION; INFECTION; RECEPTORS; FERRETS AB BackgroundThe ability of pigs to become infected with low pathogenic avian influenza (LPAI) viruses and then generate mammalian adaptable influenza A viruses is difficult to determine. Yet, it is an important link to understanding any relationship between LPAI virus ecology and possible epidemics among swine and/or humans. ObjectivesAssess susceptibility of pigs to LPAI viruses found within the United States and their direct contact transmission potential. MethodsPigs were inoculated with one of ten H5 or H7 LPAI viruses selected from seven different bird species to test infectivity, virulence, pathogenesis, and potential to transmit virus to contact pigs through histological, RRT-PCR and seroconversion data. ResultsAlthough pigs were susceptible to infection with each of the LPAI viruses, no clinical disease was recognized in any pig. During the acute phase of the infection, minor pulmonary lesions were found in some pigs and one or more pigs in each group were RRT-PCR-positive in the lower respiratory tract, but no virus was detected in upper respiratory tract (negative nasal swabs). Except for one group, one or more pigs in each LPAI group developed antibody. No LPAI viruses transmitted to contact pigs. ConclusionsLPAI strains from various bird populations within the United States are capable of infecting pigs. Although adaptability and transmission of individual strains seem unlikely, the subclinical nature of the infections demonstrates the need to improve sampling and testing methods to more accurately measure incidence of LPAI virus infection in pigs, and their potential role in human-zoonotic LPAI virus dynamics. C1 [Balzli, Charles; Suarez, David; Swayne, David E.] ARS, USDA, US Natl Poultry Res Ctr, Southeastern Poultry Res Lab, Athens, GA USA. [Lager, Kelly; Vincent, Amy; Gauger, Phillip; Brockmeier, Susan; Miller, Laura; Richt, Juergen A.; Ma, Wenjun] ARS, USDA, Natl Anim Dis Ctr, 1920 Dayton Ave,POB 70, Ames, IA 50056 USA. [Gauger, Phillip] Iowa State Univ, Coll Vet Med, Dept Vet Diagnost & Prod Anim Med, Ames, IA USA. [Richt, Juergen A.; Ma, Wenjun] Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA. RP Lager, K (reprint author), ARS, USDA, Natl Anim Dis Ctr, 1920 Dayton Ave,POB 70, Ames, IA 50056 USA. EM kelly.lager@ars.usda.gov NR 24 TC 2 Z9 2 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1750-2640 EI 1750-2659 J9 INFLUENZA OTHER RESP JI Influenza Other Respir. Viruses PD JUL PY 2016 VL 10 IS 4 BP 346 EP 352 DI 10.1111/irv.12386 PG 7 WC Infectious Diseases; Virology SC Infectious Diseases; Virology GA DQ2ZN UT WOS:000379072500012 PM 26946338 ER PT J AU Buechling, A Martin, PH Canham, CD Shepperd, WD Battaglia, MA AF Buechling, Arne Martin, Patrick H. Canham, Charles D. Shepperd, Wayne D. Battaglia, Mike A. TI Climate drivers of seed production in Picea engelmannii and response to warming temperatures in the southern Rocky Mountains SO JOURNAL OF ECOLOGY LA English DT Article DE climate change; life-history trade-offs; masting; maximum likelihood; PRISM; reproductive ecology; TopoWx ID EVOLUTIONARY ECOLOGY; POPULATION SYNCHRONY; MASTING BEHAVIOR; CONE PRODUCTION; UNITED-STATES; FOREST TREES; REPRODUCTION; GROWTH; DYNAMICS; DROUGHT AB Seed production by Picea engelmannii was monitored at 13 sites distributed across a 670m elevation gradient for 40years. Time series of annual seed output was investigated for evidence of masting behaviour and trends in seed abundance over time. We used regression models in a likelihood framework to examine climate effects on seed production for critical periods in the species' reproductive cycle. We rigorously evaluated the performance of two gridded climate data sets, PRISM and TopoWx, before using associated variables as predictors in the seed models. Seed production at these sites does not strictly conform to the classic masting concept. Seed abundance was highly variable over time and strongly synchronized among sites, but mast years could not be objectively identified due to intermediate levels of seed output. Model results indicate that climate conditions across multiple years cumulatively determine reproductive output. High seed rain is associated with elevated summer temperatures in the year that seeds are dispersed, low spring snowfall in the year preceding seed dispersal when buds are initiated, and reduced spring snowfall in a so-called priming year two years prior to seed dispersal. Low spring precipitation putatively increases growing season length and resource accumulation in seed trees. Linear models identified significant positive trends in seed output over time. Anomalous aridity and summer warmth in the latter half of the study period were highly favourable for seed production and were associated with increases in seed abundance.Synthesis. The increases in seed output observed in this study may promote population fitness of P. engelmannii in the face of changing climate regimes and increasing frequencies of fire- and insect-related tree mortality in the Rocky Mountains. Since this species lacks a persistent seed bank, re-colonization of disturbed areas or dispersal to shifting habitats depends on adequate production of seed by surviving trees, which according to these analyses may be moderately enhanced by current climate trends. However, some evidence also indicates that increases in seed output will ultimately be constrained by threshold high temperatures in the seed maturation year. C1 [Buechling, Arne; Martin, Patrick H.] Colorado State Univ, Dept Hort & Landscape Architecture, 1173 Campus Delivery, Ft Collins, CO 80523 USA. [Canham, Charles D.] Cary Inst Ecosyst Studies, Box AB, Millbrook, NY 12545 USA. [Shepperd, Wayne D.; Battaglia, Mike A.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Buechling, A (reprint author), Colorado State Univ, Dept Hort & Landscape Architecture, 1173 Campus Delivery, Ft Collins, CO 80523 USA. EM arne@rams.colostate.edu FU Colorado Forest Restoration Institute FX We gratefully acknowledge Erin Birtwistle, Kevin Bolland and Ben Gannon for assistance with field and laboratory work, Lance Asherin for years of seed collection and laboratory analyses, Kelly Elder for supplying weather station data, and Tony Cheng and the Colorado Forest Restoration Institute for important funding. We thank Lori Wahn and three anonymous referees for helpful comments. We especially thank Maureen Ryan for invaluable support. NR 76 TC 2 Z9 2 U1 21 U2 29 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-0477 EI 1365-2745 J9 J ECOL JI J. Ecol. PD JUL PY 2016 VL 104 IS 4 BP 1051 EP 1062 DI 10.1111/1365-2745.12572 PG 12 WC Plant Sciences; Ecology SC Plant Sciences; Environmental Sciences & Ecology GA DQ2ES UT WOS:000379015400017 ER PT J AU Estep, AS Sanscrainte, ND Becnel, JJ AF Estep, A. S. Sanscrainte, N. D. Becnel, J. J. TI DsRNA-mediated targeting of ribosomal transcripts RPS6 and RPL26 induces long-lasting and significant reductions in fecundity of the vector Aedes aegypti SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Aedes aegypti; Ribosome; dsRNA; siRNA; Oviposition; Fecundity; Trigger; Gene silencing ID DOUBLE-STRANDED-RNA; MOSQUITO VITELLOGENIN; GENE KNOCKDOWN; BLOOD MEAL; INTERFERENCE; EXPRESSION; DROSOPHILA; ANOPHELES; TRANSPORT; ELEGANS AB Ribosomal transcripts produce critical proteins that are involved in most cellular production processes. Targeting ribosomal transcripts has produced mortality in mites and ticks but the effect of ribosomal transcript knockdown has not been thoroughly examined in mosquitoes. We examine the effects of triggers targeting four ribosomal proteins (RP) transcripts. Although no significant mortality was observed after dsRNA microinjection and subsequent blood feeding, significant contrasts were observed on fecundity. Triggers targeting RPS6 and RPL26 effectively reduced gene expression but more importantly, reduced reproductive output by more than 96% and 91% at the first oviposition while triggers targeting RPL1 and RPS2 did not cause a reduction although gene expression was reduced. Significantly reduced fecundity continued through a second oviposition cycle in dsRPS6 and dsRPL26 cohorts, although the effect was not as strong. Relative gene expression levels confirmed specific transcript knockdown up to 20 days post-injection in mosquitoes that did not oviposit or produced reduced clutch sizes. Dissections at 36 h post-blood meal indicated defects in oocyte provisioning. The strong phenotype produced by d5RPS6 allowed us to examine the effects in various tissues as well as the dose response, trigger format, delivery method and trigger specificity in Aedes aegypti. Strong knockdown was observed in the abdomen and the ovaries. Greater than 50 ng of dsRPS6 significantly reduced fecundity but not when delivered in a sugar meal or as an siRNA. Similar bioassays with mutated d5RPS6 triggers indicates that up to three mismatches per possible siRNA are still effective in reducing fecundity. These studies indicate that while active and effective triggers can be developed for vector species, the lack of an efficient delivery method is the biggest barrier to use as a potential control method. Published by Elsevier Ltd. C1 [Estep, A. S.] Naval Air Stn, Navy Entomol Ctr Excellence, Testing & Evaluat Dept, CMAVE Detachment, Jacksonville, FL 32211 USA. [Estep, A. S.; Sanscrainte, N. D.; Becnel, J. J.] USDA ARS, Ctr Med Agr & Vet Entomol, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. RP Estep, AS (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. EM alden.estep@ars.usda.gov OI Estep, Alden/0000-0002-8281-3229 FU USDA; Navy and Marine Corps Public Health Center [WII-141C]; Deployed War Fighters Protection Program FX The authors thank Drs. James Baum, O.P. Perera, and Hanayo Arimoto for critical review of the manuscript. The authors would also like to thank Dr. Jason Puglise, Catherine McDermott, Lucy Li, and Jessica Louton for technical assistance. Funding was provided by the USDA, the WII-141C project of the Navy and Marine Corps Public Health Center, and the Deployed War Fighters Protection Program. The funders had no role in design or direction of the study or development of the manuscript. NR 47 TC 0 Z9 0 U1 2 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 EI 1879-1611 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD JUL PY 2016 VL 90 BP 17 EP 26 DI 10.1016/j.jinsphys.2016.05.001 PG 10 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA DQ3IH UT WOS:000379095500003 PM 27180677 ER PT J AU Eady, M Park, B AF Eady, M. Park, B. TI Classification of Salmonella enterica serotypes with selective bands using visible/NIR hyperspectral microscope images SO JOURNAL OF MICROSCOPY LA English DT Article DE Band selection; hyperspectral microscope; Salmonella; support vector machine ID IMAGING-SYSTEM; BIOSENSORS; IDENTIFICATION; BACTERIA; QUALITY; DIFFERENTIATION; SPECTROSCOPY; SEROGROUPS; PATHOGENS; NETWORKS AB Optical detection of foodborne bacteria such as Salmonella classifies bacteria by analysing spectral data, and has potential for rapid detection. In this experiment hyperspectral microscopy is explored as a means for classifying five Salmonella serotypes. Initially, the microscope collects 89 spectral measurements between 450 and 800 nm. Here, the objective was to develop correct classification of five serotypes with optimal spectral bands selected through multivariate data analysis (MVDA), thus reducing the data processing and storage requirement necessary for practical application in the food industry. An upright digital microscope is equipped with an acousto-optical tuneable filter, electron multiplying charge-coupled device, and metal halide lighting source. Images for each of the five serotypes were collected, and informative bands were identified through a principal component analysis, for four abbreviated spectral ranges containing 3, 7, 12 and 20 spectral bands. The experiment was repeated with an independent repetition and images were collected at each of the reduced band sets, identified by the first repetition. A support vector machine (SVM) was used to classify serotypes. Results showed that with the first repetition, classification accuracy decreased from 99.5% (89 bands) to 84.5% (3 bands), whereas the second repetition showed classification accuracies of 100%, possibly due to a reduction in spectral noise. The support vector machine regression (SVMR) was applied with cross-validation, and had R-2 calibration and validation values >0.922. Although classification accuracies through SVM classification showed that as little as 3 bands were able to classify 100% of the samples, the SVMR shows that the smallest root-mean squared-error values were 0.001 and 0.002 for 20 and 12 bands, respectively, suggesting that the 12 band range collected between 586 and 630 nm is optimal for classifying bacterial serotypes, with only the informative HMI bands selected. Lay Description Optical detection of pathogenic bacteria can offer the potential for early and rapid identification without the need for high cost molecular testing or traditional plating techniques that typically require between several days and a week to complete. Optical characteristics assess bacteria by collecting a spectral fingerprint that is unique to the organism. Here, hyperspectral microscopy is used to detect five serotypes within the Salmonella species on a cellular level. The hyperspectral microscope collects images at 89 wavelengths along the visible and near infrared light spectrums. These images are stacked on top of each other, creating a spectral fingerprint for each cell in the image. The spectral information from the cell is then processed through multivariate data analyses. A drawback of hyperspectral imaging is the large datasets that are accumulated. Theseanalyses reduce the amount of information generated from hyperspectral image cubes to easily interpreted plots and graphs. In addition to classification, the second objective was to reduce the number of wavelengths collected by the hyperspectral microscope to as few as possible, while maintaining high classification accuracy for the Salmonella cells. Images were collected for the five serotype samples, with important spectral bands identified through the multivariate analyses. Then, a second independent repetition of images were collected, but only at the spectral ranges identified as informative. Overall, images for all samples were collected with 89, 20, 12, 7 and 3 spectral bands. Multivariate classification analyses were performed on each repetition, with the first repetition seeing an overall decrease in accuracy as the bands are reduced, from 99.5% (89 bands) to 84.5% (3 bands). Eliminating the spectral bands deemed uninformative showed classification accuracies to be 100% for all reduced variable sets. Using multivariate data analyses to compare similarities between the two repetitions showed the strongest correlation with the reduced spectral range of 586-630 nm, containing 12 measured spectral bands. This, in combination with 100% classification when collecting only the informative spectral bands suggests that accurate classification can be achieved through hyperspectral microscopy within the spectral range of 586-630 nm. This is a 86.5% reduction in the data processing and storage requirement, moving towards application of this detection method. C1 [Eady, M.; Park, B.] ARS, USDA, Russell Res Ctr, POB 5677, Athens, GA 30604 USA. [Eady, M.; Park, B.] Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA. RP Park, B (reprint author), ARS, USDA, Russell Res Ctr, POB 5677, Athens, GA 30604 USA. EM bosoon.park@ars.usda.gov NR 39 TC 0 Z9 0 U1 8 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-2720 EI 1365-2818 J9 J MICROSC-OXFORD JI J. Microsc.. PD JUL PY 2016 VL 263 IS 1 BP 10 EP 19 DI 10.1111/jmi.12368 PG 10 WC Microscopy SC Microscopy GA DQ4II UT WOS:000379167100002 PM 26695128 ER PT J AU Wong, WW Ortiz, CL Stuff, JE Mikhail, C Lathan, D Moore, LA Alejandro, ME Butte, NF Smith, EO AF Wong, William W. Ortiz, Christina L. Stuff, Janice E. Mikhail, Carmen Lathan, Debra Moore, Louis A. Alejandro, Mercedes E. Butte, Nancy F. Smith, Elliot O'Brian TI A Community-based Healthy Living Promotion Program Improved Self-esteem Among Minority Children SO JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION LA English DT Article DE accelerometry; community; dietary habits; minority children; nutrition knowledge; obesity; physical activity; self-esteem ID CHILDHOOD OBESITY PREVENTION; RANDOMIZED CONTROLLED-TRIAL; SUMMER CAMP PROGRAM; BODY-MASS INDEX; QUALITY-OF-LIFE; WEIGHT-LOSS; PHYSICAL-ACTIVITY; ADOLESCENTS; QUESTIONNAIRE; INTERVENTION AB Objectives: Improving self-esteem, dietary habits, and physical activity is essential for long-term success in childhood obesity prevention. The aim is to evaluate the effects of a healthy living promotion program, Healthy Kids-Houston, on BMI, dietary habits, self-esteem, and physical activity among minority children. Methods: The after-school program was implemented at community centers in low-income neighborhoods with close proximity to public schools. The program consisted of 3 6-week sessions. Each week, children attended 2 2-hour sessions. Each 2-hour session in the intervention included 90 minutes of structured physical activities and 30 minutes of nutrition and healthy habit lessons. The control group received typical enrichment programs. Outcomes were measured before the intervention and at the end of each 6-week session. Results: We enrolled 877 children (age 10.2 +/- 0.1 years (mean +/- SE); body mass index z score: 1.49 +/- 0.1; 52.0% boys; 72.6% Hispanic) in the program with 524 children received the intervention at 14 community centers and 353 children served as control at 10 community centers. The intervention led to no improvements in BMI z score (P = 0.78) and dietary habits (P = 0.46). Significant improvements (P <= 0.02) were detected in the amount of exercise that a child perceived to be required to offset a large meal and in several key self-esteem scores. No improvements were detected in physical activities (P >= 0.21). Conclusions: The improvement in some key self-esteem scores and nutrition knowledge may act as a mediator to motivate these children to adopt a healthier lifestyle in the future. C1 [Wong, William W.; Stuff, Janice E.; Alejandro, Mercedes E.; Butte, Nancy F.; Smith, Elliot O'Brian] Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Ortiz, Christina L.; Lathan, Debra; Moore, Louis A.] City Houston, Houston Pk & Recreat Dept, Houston, TX USA. [Mikhail, Carmen] Texas Childrens Hosp, Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. RP Wong, WW (reprint author), USDA ARS Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM wwong@bcm.edu FU National Research Initiative grant from the USDA National Institute of Food and Agriculture Human Nutrition and Obesity Program [2008-55215-18875]; USDA [6250-51000-053] FX The study was supported by the National Research Initiative grant no. 2008-55215-18875 from the USDA National Institute of Food and Agriculture Human Nutrition and Obesity Program with support from the USDA grant no. 6250-51000-053. The contents of this publication do not necessarily reflect the views or policies of the USDA or City of Houston, nor does mention of trade names, commercial products, or organizations imply endorsement. NR 32 TC 0 Z9 0 U1 14 U2 17 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0277-2116 EI 1536-4801 J9 J PEDIATR GASTR NUTR JI J. Pediatr. Gastroenterol. Nutr. PD JUL PY 2016 VL 63 IS 1 BP 106 EP 112 DI 10.1097/MPG.0000000000001088 PG 7 WC Gastroenterology & Hepatology; Nutrition & Dietetics; Pediatrics SC Gastroenterology & Hepatology; Nutrition & Dietetics; Pediatrics GA DP7PX UT WOS:000378692400032 PM 27336592 ER PT J AU Rodriguez, JR Masand, P Kellermayer, R AF Rodriguez, J. Ruben Masand, Prakash Kellermayer, Richard TI Upper Endoscopic Diagnosis of a Jejunocolonic Fistula SO JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION LA English DT Editorial Material C1 [Rodriguez, J. Ruben] Texas Childrens Hosp, Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Sect Pediat Surg,Dept Surg, Houston, TX 77030 USA. [Masand, Prakash] Texas Childrens Hosp, Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Dept Pediat Radiol, Houston, TX 77030 USA. [Kellermayer, Richard] Texas Childrens Hosp, Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Sect Pediat Gastroenterol,Dept Pediat, Houston, TX 77030 USA. RP Kellermayer, R (reprint author), Baylor Coll Med, Sect Pediat Gastroenterol Hepatol & Nutr, 6621 Fannin St,CC1010-00, Houston, TX 77030 USA. EM kellerma@bcm.edu NR 3 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0277-2116 EI 1536-4801 J9 J PEDIATR GASTR NUTR JI J. Pediatr. Gastroenterol. Nutr. PD JUL PY 2016 VL 63 IS 1 BP E14 EP E14 DI 10.1097/MPG.0000000000000517 PG 1 WC Gastroenterology & Hepatology; Nutrition & Dietetics; Pediatrics SC Gastroenterology & Hepatology; Nutrition & Dietetics; Pediatrics GA DP7PX UT WOS:000378692400003 PM 25079482 ER PT J AU Benestan, LM Ferchaud, AL Hohenlohe, PA Garner, BA Naylor, GJP Baums, IB Schwartz, MK Kelley, JL Luikart, G AF Benestan, Laura Marilyn Ferchaud, Anne-Laure Hohenlohe, Paul A. Garner, Brittany A. Naylor, Gavin J. P. Baums, Iliana Brigitta Schwartz, Michael K. Kelley, Joanna L. Luikart, Gordon TI Conservation genomics of natural and managed populations: building a conceptual and practical framework SO MOLECULAR ECOLOGY LA English DT Editorial Material DE conservation genomics; effective population size; genetic population structure; local adaptation; massively parallel sequencing; SNPs discovery and filtering ID GENERATION SEQUENCING DATA; SALMON ONCORHYNCHUS-TSHAWYTSCHA; NON-MODEL ORGANISMS; NEXT-GENERATION; LINKAGE DISEQUILIBRIUM; MARINE POPULATIONS; DUPLICATION EVENT; GENETIC DIVERSITY; PROGRAM STRUCTURE; LOCAL ADAPTATION AB The boom of massive parallel sequencing (MPS) technology and its applications in conservation of natural and managed populations brings new opportunities and challenges to meet the scientific questions that can be addressed. Genomic conservation offers a wide range of approaches and analytical techniques, with their respective strengths and weaknesses that rely on several implicit assumptions. However, finding the most suitable approaches and analysis regarding our scientific question are often difficult and time-consuming. To address this gap, a recent workshop entitled 'ConGen 2015' was held at Montana University in order to bring together the knowledge accumulated in this field and to provide training in conceptual and practical aspects of data analysis applied to the field of conservation and evolutionary genomics. Here, we summarize the expertise yield by each instructor that has led us to consider the importance of keeping in mind the scientific question from sampling to management practices along with the selection of appropriate genomics tools and bioinformatics challenges. C1 [Benestan, Laura Marilyn; Ferchaud, Anne-Laure] Univ Laval, IBIS, Dept Biol, Quebec City, PQ G1V 0A6, Canada. [Hohenlohe, Paul A.] Univ Idaho, Inst Bioinformat & Evolutionary Studies, Moscow, ID 83844 USA. [Garner, Brittany A.; Luikart, Gordon] Univ Montana, Div Biol Sci, Flathead Lake Biol Stn, Fish & Wildlife Genom Grp, Missoula, MT 59812 USA. [Garner, Brittany A.; Luikart, Gordon] Univ Montana, Coll Forestry & Conservat, Wildlife Program, Fish & Wildlife Genom Grp, Missoula, MT 59812 USA. [Naylor, Gavin J. P.] Univ S Carolina, Coll Charleston & Med, Hollings Marine Lab, 331 Ft Johnson Rd, Charleston, SC 29412 USA. [Baums, Iliana Brigitta] Penn State Univ, Dept Biol, Mueller Lab 208, University Pk, PA USA. [Schwartz, Michael K.] USDA Forest Serv, Natl Genom Ctr Wildlife & Fish Conservat, 800 E Beckwith Ave, Missoula, MT 59801 USA. [Kelley, Joanna L.] Washington State Univ, Sch Biol Sci, Pullman, WA 99164 USA. RP Ferchaud, AL (reprint author), Univ Laval, IBIS, Dept Biol, Quebec City, PQ G1V 0A6, Canada. EM annelaureferchaud@gmail.com RI Baums, Iliana/G-6435-2010; OI Baums, Iliana/0000-0001-6463-7308; Kelley, Joanna/0000-0002-7731-605X NR 115 TC 5 Z9 5 U1 33 U2 57 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1083 EI 1365-294X J9 MOL ECOL JI Mol. Ecol. PD JUL PY 2016 VL 25 IS 13 BP 2967 EP 2977 DI 10.1111/mec.13647 PG 11 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA DQ1DT UT WOS:000378942200003 PM 27086132 ER PT J AU Kostka, JE Weston, DJ Glass, JB Lilleskov, EA Shaw, AJ Turetsky, MR AF Kostka, Joel E. Weston, David J. Glass, Jennifer B. Lilleskov, Erik A. Shaw, A. Jonathan Turetsky, Merritt R. TI The Sphagnum microbiome: new insights from an ancient plant lineage SO NEW PHYTOLOGIST LA English DT Review DE bacteria; fungi; methanotroph; microbiome; nitrogen fixation; peatland; plant growth promotion; Sphagnum ID RHIZOSPHERE MICROBIOME; BACTERIAL COMMUNITIES; METHANE PRODUCTION; GLOBAL CHANGE; RAISED BOGS; NITROGEN; MOSSES; CARBON; PEATLANDS; METHANOTROPHS AB Peat mosses of the genus Sphagnum play a major role in global carbon storage and dominate many northern peatland ecosystems, which are currently being subjected to some of the most rapid climate changes on Earth. A rapidly expanding database indicates that a diverse community of microorganisms is intimately associated with Sphagnum, inhabiting the tissues and surface of the plant. Here we summarize the current state of knowledge regarding the Sphagnum microbiome and provide a perspective for future research directions. Although the majority of the microbiome remains uncultivated and its metabolic capabilities uncharacterized, prokaryotes and fungi have the potential to act as mutualists, symbionts, or antagonists of Sphagnum. For example, methanotrophic and nitrogen-fixing bacteria may benefit the plant host by providing up to 20-30% of Sphagnum carbon and nitrogen, respectively. Next-generation sequencing approaches have enabled the detailed characterization of microbiome community composition in peat mosses. However, as with other ecologically or economically important plants, our knowledge of Sphagnum-microbiome associations is in its infancy. In order to attain a predictive understanding of the role of the microbiome in Sphagnum productivity and ecosystem function, the mechanisms of plant-microbiome interactions and the metabolic potential of constituent microbial populations must be revealed. C1 [Kostka, Joel E.; Glass, Jennifer B.] Georgia Inst Technol, Sch Biol Sci, Atlanta, GA 30332 USA. [Kostka, Joel E.; Glass, Jennifer B.] Georgia Inst Technol, Sch Earth & Atmospher Sci, Atlanta, GA 30332 USA. [Weston, David J.] Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA. [Lilleskov, Erik A.] US Forest Serv, No Res Stn, USDA, Houghton, MI 49931 USA. [Shaw, A. Jonathan] Duke Univ, Dept Biol, Durham, NC 27708 USA. [Turetsky, Merritt R.] Univ Guelph, Dept Integrat Biol, Guelph, ON N1G 2W1, Canada. RP Kostka, JE (reprint author), Georgia Inst Technol, Sch Biol Sci, Atlanta, GA 30332 USA.; Kostka, JE (reprint author), Georgia Inst Technol, Sch Earth & Atmospher Sci, Atlanta, GA 30332 USA. EM joel.kostka@biology.gatech.edu FU US Department of Energy, Office of Science, Office of Biological and Environmental Research; Terrestrial Ecosystem Science (TES) Program under US Department of Energy [DE-SC0012088]; US Department of Energy [DE-AC05-00OR22725] FX This review was supported in part by the US Department of Energy, Office of Science, Office of Biological and Environmental Research. J.E.K. was supported by the Terrestrial Ecosystem Science (TES) Program, under US Department of Energy contract # DE-SC0012088. Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the US Department of Energy under contract DE-AC05-00OR22725. NR 61 TC 2 Z9 2 U1 22 U2 41 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD JUL PY 2016 VL 211 IS 1 BP 57 EP 64 DI 10.1111/nph.13993 PG 8 WC Plant Sciences SC Plant Sciences GA DQ4ZE UT WOS:000379212800007 PM 27173909 ER PT J AU Friedman, AN Kim, J Kaiser, S Pedersen, TL Newman, JW Watkins, BA AF Friedman, Alton N. Kim, Jeffrey Kaiser, Shaun Pedersen, Theresa L. Newman, John W. Watkins, Bruce A. TI Association between plasma endocannabinoids and appetite in hemodialysis patients: A pilot study SO NUTRITION RESEARCH LA English DT Article DE Endocannabinoids; Hemodialysis; Mass spectrometry; Omega-3; Omega-6; Polyunsaturated fatty acids ID FOOD-INTAKE; FATTY-ACIDS; BRAIN; OLEOYLETHANOLAMIDE; MOLECULES; ANOREXIA; DISEASE; TOOLS AB Uremia-associated anorexia may be related to altered levels of long chain n-6 and n-3 polyunsaturated fatty acid (PUFA) derived circulating endocannabinoids (EC) and EC-like compounds that are known to mediate appetite. Our study's hypothesis was that such molecules are associated with appetite in patients with end-stage renal disease. A cross-sectional observational study was performed in 20 chronic hemodialysis patients (9 females, 11 males) and 10 healthy female controls in whom appetite was assessed using the Simplified Nutritional Appetite Questionnaire (SNAQ) and blood drawn in the fasting (and when applicable) pre-dialysis state. Blood levels of PUFA and EC were also measured. Higher blood levels of the long chain n-6 fatty acid 20:4n6 (arachidonic acid) and lower levels of the long chain n-3 fatty acid 20:5n3 (eicosapentaenoic acid) were observed in female hemodialysis patients compared to controls. No differences were observed between male and female patients. In female study participants strong correlations between specific EC-like compounds and total SNAQ scores were noted, including with the n-6 PUFA derived linoleoyl ethanolamide (L-EA; rho = -0.60, P < .01) and the n-3 PUFA derived docosahexaenoyl ethanolamide (DH-EA; rho = 0.63, P < .01). The L-EADH-EA ratio was most strongly associated with the SNAQ score (rho = -0.74, P <= .001), and its questions associated with appetite (rho = -0.69, P <= .01) and satiety (rho = -0.81, P <= .001). These findings support a link between circulating EC and appetite in hemodialysis patients. (C) 2016 Elsevier Inc. All rights reserved. C1 [Friedman, Alton N.] Indiana Univ Sch Med, Indianapolis, IN 46202 USA. [Kim, Jeffrey] Univ Calif Davis, Genome & Biomed Sci Facil, Davis, CA 95616 USA. [Kaiser, Shaun] Texas Kidney Inst, Dallas, TX 75230 USA. [Pedersen, Theresa L.; Newman, John W.] ARS, Obes & Metab Res Unit, USDA, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [Newman, John W.] Univ Calif Davis, West Coast Metabol Ctr, Davis, CA 95616 USA. [Newman, John W.; Watkins, Bruce A.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Watkins, Bruce A.] Univ Connecticut, Ctr Aging, Ctr Hlth, Farmington, CT 06030 USA. RP Friedman, AN (reprint author), 550 Univ Blvd,Suite 6100, Indianapolis, IN 46202 USA. EM allfried@iu.edu FU USDA Intramural Projects [5306-51530-019-00D, 2032-5130-022-00D]; West Coast Metabolomics Center Grant NIH [U24 DK097154] FX The study was supported by funds to BAW for the metabolomics collaborations at the University of Connecticut Health Center and Center on Aging. Additional support was provided by the USDA Intramural Projects 5306-51530-019-00D and 2032-5130-022-00D, and the West Coast Metabolomics Center Grant NIH U24 DK097154. The USDA is an equal opportunity provider and employer. The authors have no conflict of interest to report. NR 26 TC 0 Z9 0 U1 2 U2 5 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0271-5317 J9 NUTR RES JI Nutr. Res. PD JUL PY 2016 VL 36 IS 7 BP 658 EP 662 DI 10.1016/j.nutres.2016.03.009 PG 5 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DQ3LI UT WOS:000379103400004 PM 27333956 ER PT J AU Zhu, CHH Gertz, ER Cai, YM Burri, BJ AF Zhu, Chenghao H. Gertz, Erik R. Cai, Yimeng Burri, Betty J. TI Consumption of canned citrus fruit meals increases human plasma beta-cryptoxanthin concentration, whereas lycopene and beta-carotene concentrations did not change in healthy adults SO NUTRITION RESEARCH LA English DT Article DE Carotenoid; Plasma response; Citrus; Human; beta-Cryptoxanthin; Lycopene ID IN-VITRO BIOACCESSIBILITY; SINGLE-NUCLEOTIDE POLYMORPHISMS; VITAMIN-A; TISSUE DISTRIBUTION; GENETIC-VARIANTS; DOUBLE-TRACER; SWEET-POTATO; DIETARY-FAT; BIOAVAILABILITY; METABOLISM AB Several studies suggest that beta-cryptoxanthin has a greater plasma response from its common food sources than other carotenoids such as beta-carotene and lycopene. The hypothesis of this study is that changes in plasma beta-cryptoxanthin concentrations will be greater than changes in plasma beta-carotene or lycopene concentrations even if these carotenoids are fed in a similar food matrix, such as citrus fruit. We tested this hypothesis by measuring changes in plasma concentrations of beta-cryptoxanthin, lycopene, and beta-carotene after feeding measured amounts of canned tangerines and pink grapefruit to healthy nonsmoking adult humans. Volunteers served as their own controls and received both citrus fruit treatments randomly. In the first study, 8 subjects ate single meals of 234304 g of tangerines or 60-540 g of pink grapefruit. The second study compared changes in plasma carotenoid concentration caused by feeding 234 g of tangerines or 540 g of pink grapefruit to 11 subjects. Blood was collected 5 times within 24 hours after each citrus meal. Carotenoid concentrations were analyzed by reversed-phase high-performance liquid chromatography. Plasma beta-cryptoxanthin concentrations increased within 5 hours and then stabilized, remaining high throughout the 24 hours measured. Plasma concentrations of lycopene and beta-carotene did not change. These results show that beta-cryptoxanthin concentrations increased after a citrus fruit meal, but lycopene and beta-carotene concentrations did not change after a similar citrus fruit meal. These results support our hypothesis that changes in plasma beta-cryptoxanthin are greater than changes in plasma lycopene or beta-carotene, even when these carotenoids are fed in a similar food matrix. Published by Elsevier Inc. C1 [Zhu, Chenghao H.; Cai, Yimeng; Burri, Betty J.] Univ Calif Davis, 1 Shields Ave,430 W Hlth Sci Dr, Davis, CA 95616 USA. [Gertz, Erik R.; Burri, Betty J.] ARS, Western Human Nutr Res Ctr, USDA, 430 W Hlth Sci Dr, Davis, CA 95616 USA. RP Burri, BJ (reprint author), ARS, Western Human Nutr Res Ctr, USDA, 430 W Hlth Sci Dr, Davis, CA 95616 USA. EM chzhu@ucdavis.edu; erik.gertz@ars.usda.gov; ymcai@ucdavis.edu; bjburri@ucdavis.edu FU in-house funds of Western Human Nutrition Research Center, United States Department of Agriculture FX We thank Michael La Frano, Ellen Bonnel, Leslie Woodhouse, Evelyn Jenner, and Mary Ellen Rivera for their assistance with recruiting, screening, and analyzing samples from this study. This research was supported by in-house funds of the Western Human Nutrition Research Center, United States Department of Agriculture. NR 55 TC 1 Z9 1 U1 4 U2 6 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0271-5317 J9 NUTR RES JI Nutr. Res. PD JUL PY 2016 VL 36 IS 7 BP 679 EP 688 DI 10.1016/j.nutres.2016.03.005 PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DQ3LI UT WOS:000379103400007 PM 27333959 ER PT J AU Wong, WW Strizich, G Heo, M Heymsfield, SB Himes, JH Rock, CL Gellman, MD Siega-Riz, AM Sotres-Alvarez, D Davis, SM Arredondo, EM Van Horn, L Wylie-Rosett, J Sanchez-Johnsen, L Kaplan, RC Mossavar-Rahmani, Y AF Wong, William W. Strizich, Garrett Heo, Moonseong Heymsfield, Steven B. Himes, John H. Rock, Cheryl L. Gellman, Marc D. Siega-Riz, Anna Maria Sotres-Alvarez, Daniela Davis, Sonia M. Arredondo, Elva M. Van Horn, Linda Wylie-Rosett, Judith Sanchez-Johnsen, Lisa Kaplan, Robert C. Mossavar-Rahmani, Yasmin TI Relationship Between Body Fat and BMI in a US Hispanic Population-Based Cohort Study: Results from HCHS/SOL SO OBESITY LA English DT Article ID MASS INDEX; 4-COMPARTMENT MODEL; RISK-FACTORS; OBESITY; LATINOS; ADULTS; OVERWEIGHT; O-18; ANTHROPOMETRY; DEUTERIUM AB Objective: To evaluate the percentage of body fat (%BF)-BMI relationship, identify %BF levels corresponding to adult BMI cut points, and examine %BF-BMI agreement in a diverse Hispanic/Latino population. Methods: %BF by bioelectrical impedance analysis was corrected against %BF by O-18 dilution in 434 participants of the ancillary Hispanic Community Health Study/Study of Latinos. Corrected %BF was regressed against 1/BMI in the parent study (n = 15,261), fitting models for each age group, by sex, and Hispanic/Latino background; predicted %BF was then computed for each BMI cut point. Results: Bioelectrical impedance analysis underestimated %BF by 8.7 +/- 0.3% in women and 4.6 +/- 0.3% in men (P < 0.0001). The %BF-BMI relationship was nonlinear and linear for 1/BMI. Sex-and age-specific regression parameters between %BF and 1/BMI were consistent across Hispanic/Latino backgrounds (P > 0.05). The precision of the %BF-1/BMI association weakened with increasing age in men but not women. The proportion of participants classified as nonobese by BMI but as having obesity by %BF was generally higher among women and older adults (16.4% in women vs. 12.0% in men aged 50-74 years). Conclusions: %BF was linearly related to 1/BMI with consistent relationship across Hispanic/Latino backgrounds. BMI cut points consistently underestimated the proportion of Hispanics/Latinos with excess adiposity. C1 [Wong, William W.] Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Strizich, Garrett; Heo, Moonseong; Wylie-Rosett, Judith; Kaplan, Robert C.; Mossavar-Rahmani, Yasmin] Albert Einstein Coll Med, Dept Epidemiol & Populat Hlth, Bronx, NY 10467 USA. [Heymsfield, Steven B.] Pennington Biomed Res Ctr, 6400 Perkins Rd, Baton Rouge, LA 70808 USA. [Himes, John H.] Univ Minnesota, Sch Publ Hlth, Div Epidemiol & Community Hlth, Minneapolis, MN USA. [Rock, Cheryl L.] UCSD, Sch Med, Dept Family Med & Publ Hlth, La Jolla, CA USA. [Gellman, Marc D.] Univ Miami, Dept Psychol, Behav Med Res Ctr, POB 248185, Coral Gables, FL 33124 USA. [Siega-Riz, Anna Maria] Univ North Carolina Chapel Hill, Gillings Sch Global Publ Hlth, Dept Epidemiol, Chapel Hill, NC USA. [Siega-Riz, Anna Maria] Univ North Carolina Chapel Hill, Gillings Sch Global Publ Hlth, Dept Nutr, Chapel Hill, NC USA. [Sotres-Alvarez, Daniela; Davis, Sonia M.] Univ North Carolina Chapel Hill, Gillings Sch Global Publ Hlth, Dept Biostat, Collaborat Studies Coordinating Ctr, Chapel Hill, NC USA. [Arredondo, Elva M.] San Diego State Univ, Grad Sch Publ Hlth, San Diego, CA 92182 USA. [Van Horn, Linda] Northwestern Univ, Dept Prevent Med, Feinberg Sch Med, Chicago, IL 60611 USA. [Sanchez-Johnsen, Lisa] Univ Illinois, Coll Med, Dept Psychiat & Surg, Chicago, IL USA. RP Wong, WW (reprint author), Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. EM wwong@bcm.edu RI DeMarcus, Forrest/O-9190-2016; Sotres-Alvarez, Daniela/O-9085-2016; OI Sotres-Alvarez, Daniela/0000-0002-3226-6140; Heymsfield, Steven/0000-0003-1127-9425 FU National Heart, Lung, and Blood Institute (NHLBI) [11414319-R01HL095856]; NHLBI [N01-HC65233]; University of Miami [N01-HC65234]; Albert Einstein College of Medicine [N01-HC65235]; Northwestern University [N01-HC65236]; San Diego State University [N01-HC65237]; HCHS/SOL; National Center for Advancing Translational Sciences at the NIH [UL1 TR001073] FX The Study of Latinos: Nutrition & Physical Activity Assessment Study (SOLNAS) was supported by grant 11414319-R01HL095856 from the National Heart, Lung, and Blood Institute (NHLBI). The Hispanic Community Health Study/Study of Latinos (HCHS/SOL) was carried out as a collaborative study supported by contracts from the NHLBI to the University of North Carolina (N01-HC65233), University of Miami (N01-HC65234), Albert Einstein College of Medicine (N01-HC65235), Northwestern University (N01-HC65236), and San Diego State University (N01-HC65237). The following Institutes/Centers/Offices contribute to the HCHS/SOL through a transfer of funds to the NHLBI: National Institute on Minority Health and Health Disparities, National Institute on Deafness and Other Communication Disorders, National Institute of Dental and Craniofacial Research, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Neurological Disorders and Stroke, NIH Institution-Office of Dietary Supplements. Additional support at the Albert Einstein College of Medicine was provided from the Clinical & Translational Science Award (UL1 TR001073) from the National Center for Advancing Translational Sciences at the NIH. NR 25 TC 0 Z9 0 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1930-7381 EI 1930-739X J9 OBESITY JI Obesity PD JUL PY 2016 VL 24 IS 7 BP 1561 EP 1571 DI 10.1002/oby.21495 PG 11 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA DQ6GT UT WOS:000379303200022 PM 27184359 ER PT J AU Verma, SK Lindsay, DS Rosenthal, BM Dubey, JP AF Verma, Shiv K. Lindsay, David S. Rosenthal, Benjamin M. Dubey, Jitender P. TI Ancient, globally distributed lineage of Sarcocystis from sporocysts of the Eastern rat snake (Pantherophis alleghaniensis) and its relation to neurological sequalae in intermediate hosts SO PARASITOLOGY RESEARCH LA English DT Article DE Sarcocystis; Rat snake; Mice; Host; Bioassay ID THOMAS CRICETIDAE MICROTINAE; PHYLOGENETIC-RELATIONSHIPS; APICOMPLEXA SARCOCYSTIDAE; N. SP; NESBITTI; OUTBREAK; SPP.; INFECTION; MYOSITIS; RACCOONS AB There is an emerging concern that snakes are definitive hosts of certain species of Sarcocystis that cause muscular sarcocystosis in human and non-human primates. Other species of Sarcocystis are known to cycle among snakes and rodents, but have been poorly characterized in the USA and elsewhere. Although neurological sequalae are known for certain species of Sarcocystis, no such neurological symptoms are known to typify parasites that naturally cycle in rodents. Here, sporocysts of a species of Sarcocystis were found in the intestinal contents of a rat snake (Pantherophis alleghaniensis) from Maryland, USA. The sporocysts were orally infective for interferon gamma gene knockout (KO) mice, but not to Swiss Webster outbred mice. The KO mice developed neurological signs, and were necropsied between 33 and 52 days post-inoculation. Only schizonts/merozoites were found, and they were confined to the brain. The predominant lesion was meningoencephalitis characterized by perivascular cuffs, granulomas, and necrosis of the neuropil. The schizonts and merozoites were located in neuropil, and apparently extravascular. Brain homogenates from infected KO mice were infective to KO mice and CV-1 cell line. DNA extracted from the infected mouse brain, and infected cell cultures revealed the highest identity with Sarcocystis species that employ snakes as definitive hosts. This is the first report of Sarcocystis infection in the endangered rat snake (P. alleghaniensis) and the first report of neurological sarcocystosis in mice induced by feeding sporocysts from a snake. These data underscore the likelihood that parasites in this genus that employ snakes as their definitive hosts constitute an ancient, globally distributed monophyletic group. These data also raise the possibility that neurological sequalae may be more common in intermediate hosts of Sarcocystis spp. than has previously been appreciated. C1 [Verma, Shiv K.; Rosenthal, Benjamin M.; Dubey, Jitender P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. [Lindsay, David S.] Virginia Tech, Dept Biomed Sci & Pathol, Virginia Maryland Reg Coll Vet Med, 1410 Prices Fork Rd, Blacksburg, VA 24061 USA. RP Dubey, JP (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. EM Jitender.Dubey@ars.usda.gov OI Rosenthal, Benjamin/0000-0002-0224-3773 NR 22 TC 0 Z9 0 U1 3 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 EI 1432-1955 J9 PARASITOL RES JI Parasitol. Res. PD JUL PY 2016 VL 115 IS 7 BP 2697 EP 2704 DI 10.1007/s00436-016-5086-2 PG 8 WC Parasitology SC Parasitology GA DQ2MO UT WOS:000379036600019 PM 27130321 ER PT J AU Calero-Bernal, R Cerqueira-Cezar, CK Verma, SK Mowery, J Carmena, D Beckmen, K Dubey, JP AF Calero-Bernal, Rafael Cerqueira-Cezar, Camila K. Verma, Shiv Kumar Mowery, Joseph Carmena, David Beckmen, Kimberlee Dubey, Jitender P. TI Sarcocystis arctica (Apicomplexa: Sarcocystidae): ultrastructural description and its new host record, the Alaskan wolf (Canis lupus) SO PARASITOLOGY RESEARCH LA English DT Article DE Alaskan wolf; Canis lupus; Sarcocystis; Ultrastructure; Phylogeny ID MOLECULAR CHARACTERISTICS; N. SP AB Sarcocystis sarcocysts are common in muscles of herbivores but are rare in muscles of carnivores. Here, we report sarcocysts in the muscles of a gray wolf (Canis lupus) from Alaska, USA, for the first time. Sarcocysts extracted from the tongue of the wolf were up to 900 mu m long and slender and appeared to have a relatively thin wall by light microscope. By transmission electron microscopy, the sarcocyst wall most closely resembled "type 9c," and had a wavy parasitophorous vacuolar membrane folded as pleomorphic villar protrusions (vp), with anastomoses of tips. The vp and the ground substance (gs) layer were smooth without tubules or granules. The gs was up to 2.0 mu m thick. The total width of the wall including vp and the gs was 3.5 mu m. The vp were up to 1.5 mu m long. Mature sarcocysts contained numerous bradyzoites and few metrocytes. The bradyzoites were 9.5 mu m long and 1.5 mu m wide, and contained all organelles found in Sarcocystis bradyzoites with at least two rhoptries. Molecular characterization showed the highest identity for 18S rRNA, 28S rRNA, ITS-1, and cox1 sequences of Sarcocystis arctica of the Arctic fox (Vulpes lagopus) from Norway. The ultrastructure of S. arctica from the fox is unknown. Here, we provide ultrastructure of S. arctica from the Alaskan wolf for the first time. The definitive host of S. arctica remains unknown. C1 [Calero-Bernal, Rafael; Cerqueira-Cezar, Camila K.; Verma, Shiv Kumar; Dubey, Jitender P.] ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, USDA, Bldg 1001, Beltsville, MD 20705 USA. [Mowery, Joseph] ARS, Beltsville Agr Res Ctr, Electron & Confocal Microscopy Unit, USDA, Bldg 12, Beltsville, MD 20705 USA. [Carmena, David] Carlos III Hlth Inst, Natl Ctr Microbiol, Parasitol Serv, Ctra Majadahonda Pozuelo Km 2, Madrid 28220, Spain. [Beckmen, Kimberlee] Alaska Dept Fish & Game, Wildlife Hlth & Dis Surveillance Program, Div Wildlife Conservat, 1300 Coll Rd, Fairbanks, AK 99701 USA. RP Dubey, JP (reprint author), ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, USDA, Bldg 1001, Beltsville, MD 20705 USA. EM Jitender.Dubey@ars.usda.gov OI Carmena, David/0000-0002-4015-8553 NR 9 TC 0 Z9 0 U1 3 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 EI 1432-1955 J9 PARASITOL RES JI Parasitol. Res. PD JUL PY 2016 VL 115 IS 7 BP 2893 EP 2897 DI 10.1007/s00436-016-5067-5 PG 5 WC Parasitology SC Parasitology GA DQ2MO UT WOS:000379036600040 PM 27112760 ER PT J AU Egan, JM Sloughter, JM Cardoso, T Trainor, P Wu, K Safford, H Fournier, D AF Egan, Joel M. Sloughter, J. McLean Cardoso, Tamre Trainor, Patrick Wu, Ke Safford, Hugh Fournier, Dave TI Multi-temporal ecological analysis of Jeffrey pine beetle outbreak dynamics within the Lake Tahoe Basin SO POPULATION ECOLOGY LA English DT Article DE Bark beetles; Dendroctonus jeffreyi; Disturbance ecology; Epidemiology; Spatial aggregation; Tree physiology ID TREE MORTALITY; UNITED-STATES; DENDROCTONUS-PONDEROSAE; STAND STRUCTURE; MOUNTAIN; FORESTS; DROUGHT; DISTURBANCES; INFESTATIONS; POPULATIONS AB From 1991 to 1996, Jeffrey pine beetles (Dendroctonus jeffreyi Hopkins) (JPB) caused tree mortality throughout the Lake Tahoe Basin during a severe drought. Census data were collected annually on 10,721 trees to assess patterns of JPB-caused mortality. This represents the most extensive tree-level, spatiotemporal dataset collected to-date documenting bark beetle activity. Our study was an exploratory assessment of characteristics associated with the probability of successful JPB mass-attack (P (JPB)) and group aggregation behavior that occurred throughout various outbreak phases. Numerous characteristics associated with P (JPB) varied by outbreak phase although population pressure and forest density had positive associations during all phases. During the incipient phase, JPBs caused mortality in individual trees and small groups within toeslope topographic positions and P (JPB) had a negative relationship with stem diameter. In the epidemic phase, JPB activity occurred in all topographic positions and caused mortality in spatially expanding clusters. P (JPB) had a curvilinear relationship with tree diameter and a negative relationship with proximity to nearest brood tree. Majority (92-96 %) of mass-attacked trees were within 30 m of a brood tree during the peak epidemic years. During the post-epidemic phase, mortality clusters progressively decreased while dispersal distances between mass-attacked and brood trees increased. Post-epidemic P (JPB) had a negative relationship with stem diameter and mortality was concentrated in the mid and upper-slope topographic positions. Results indicate mortality predictions are reasonable for the epidemic phase but not for incipient and post-epidemic phases. Ecological factors influencing JPB-caused tree mortality, clustered mortality patterns, and transitions from environmental to dynamic determinism are discussed. C1 [Egan, Joel M.] US Forest Serv, Forest Hlth Protect, USDA, Missoula, MT USA. [Sloughter, J. McLean] Seattle Univ, Dept Math, Seattle, WA 98122 USA. [Cardoso, Tamre] TerraStat Consulting Grp, Seattle, WA USA. [Trainor, Patrick] Univ Louisville, Div Cardiovasc Med, Louisville, KY 40292 USA. [Wu, Ke] Univ Montana, Dept Math Sci, Missoula, MT 59812 USA. [Safford, Hugh] US Forest Serv, Pacific Southwest Reg, USDA, Vallejo, CA USA. [Safford, Hugh] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. [Fournier, Dave] US Forest Serv, Lake Tahoe Basin Management Unit, USDA, South Lake Tahoe, CA USA. [Egan, Joel M.] 26 Ft Missoula Rd, Missoula, MT 59804 USA. RP Egan, JM (reprint author), US Forest Serv, Forest Hlth Protect, USDA, Missoula, MT USA.; Egan, JM (reprint author), 26 Ft Missoula Rd, Missoula, MT 59804 USA. EM jegan@fs.fed.us FU U.S. Forest Service, Forest Health Protection through a Standard Technology Development Program grant [R5-2010-05]; Seattle University FX This study could not have occurred without the vision, study area establishment, and monitoring surveys pioneered by John Wenz, retired U.S. Forest Service. This work was funded by the U.S. Forest Service, Forest Health Protection through a Standard Technology Development Program grant (R5-2010-05). Additional funding was provided by Seattle University's 2011 College of Science and Engineering Undergraduate Research Program. This manuscript was improved substantially following comments provided by Russell Parsons and two peers that provided anonymous review. This work could not have been completed without collaboration with the Nevada State Division of Forestry that allowed pest monitoring on state-managed lands. Our special thanks go to Susan Skalski for agreement support, Jim Schmidt for GIS support, Tashia Steele for assistance with coordinate validation, and Martin MacKenzie, Beverly Bulaon, Danny Cluck, Sheri Smith, and Gregg DeNitto for supporting this study. NR 82 TC 1 Z9 1 U1 14 U2 14 PU SPRINGER JAPAN KK PI TOKYO PA CHIYODA FIRST BLDG EAST, 3-8-1 NISHI-KANDA, CHIYODA-KU, TOKYO, 101-0065, JAPAN SN 1438-3896 EI 1438-390X J9 POPUL ECOL JI Popul. Ecol. PD JUL PY 2016 VL 58 IS 3 BP 441 EP 462 DI 10.1007/s10144-016-0545-2 PG 22 WC Ecology SC Environmental Sciences & Ecology GA DQ3DI UT WOS:000379082600010 ER PT J AU Mebratu, YA Smith, KR Agga, GE Tesfaigzi, Y AF Mebratu, Yohannes A. Smith, Kevin R. Agga, Getahun E. Tesfaigzi, Yohannes TI Inflammation and emphysema in cigarette smoke-exposed mice when instilled with poly (I:C) or infected with influenza A or respiratory syncytial viruses SO RESPIRATORY RESEARCH LA English DT Article DE Influenza A virus; Respiratory syncytial virus; Poly (I:C); Emphysema; Mice; Cigarette smoke; Mouse ID OBSTRUCTIVE PULMONARY-DISEASE; SMALL-AIRWAY OBSTRUCTION; IMMUNE-RESPONSES; INNATE IMMUNE; UP-REGULATION; MOUSE MODEL; H1N1 VIRUS; TH17 CELLS; COPD; EXACERBATIONS AB Background: The length of time for cigarette smoke (CS) exposure to cause emphysema in mice is drastically reduced when CS exposure is combined with viral infection. However, the extent of inflammatory responses and lung pathologies of mice exposed to CS and infected with influenza A virus (IAV), respiratory syncytial virus (RSV), or treated with the viral derivative dsRNA (polyinosine-polycytidylic acid [poly (I:C)] have not been compared. Methods: Mice were exposed to CS or filtered air for 4 weeks and received a single dose of vehicle, AV, or RSV infection and extent of inflammation and emphysema was evaluated 14 d later. In another set of experiments, mice were instilled with poly (I:C) twice a week during the third and fourth weeks of CS exposure and immediately analyzed for extent of inflammation and lung pathologies. Results: In CS-exposed mice, inflammation was characterized mainly by macrophages, lymphocytes, and neutrophils after IAV infection, mainly by lymphocytes, and neutrophils after RSV infection, and mainly by lymphocytes and neutrophils after poly (I:C) instillations. Despite increased inflammation, extent of emphysema by poly (I:C) was very mild; but was robust and similar for both IAV and RSV infections with enhanced MMP-12 mRNA expression and TUNEL positivity. Both IAV and RSV infections increased the levels of IL-17, IL-1 beta, IL-12b, IL-18, IL-23a, Ccl-2, Ccl-7 mRNAs in the lungs of CS-exposed mice with IAV causing more increases than RSV. Conclusion: CS-induced inflammatory responses and extent of emphysematous changes differ depending on the type of viral infection. These animal models may be useful to study the mechanisms by which different viruses exacerbate CS-induced inflammation and emphysema. C1 [Mebratu, Yohannes A.; Smith, Kevin R.; Tesfaigzi, Yohannes] Lovelace Resp Res Inst, COPD Program, 2425 Ridgecrest Dr SE, Albuquerque, NM 87108 USA. [Agga, Getahun E.] ARS, USDA, Clay Ctr, NE USA. RP Mebratu, YA (reprint author), Lovelace Resp Res Inst, COPD Program, 2425 Ridgecrest Dr SE, Albuquerque, NM 87108 USA. EM ymebratu@lrri.org NR 77 TC 0 Z9 0 U1 2 U2 2 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1465-993X EI 1465-9921 J9 RESP RES JI Respir. Res. PD JUL 1 PY 2016 VL 17 AR 75 DI 10.1186/s12931-016-0392-x PG 12 WC Respiratory System SC Respiratory System GA DQ5SI UT WOS:000379264900001 PM 27363862 ER PT J AU Swartley, OM Foley, JF Livingston, DP Cullen, JM Elmore, SA AF Swartley, Olivia M. Foley, Julie F. Livingston, David P., III Cullen, John M. Elmore, Susan A. TI Histology Atlas of the Developing Mouse Hepatobiliary Hemolymphatic Vascular System with Emphasis on Embryonic Days 11.5-18.5 and Early Postnatal Development SO TOXICOLOGIC PATHOLOGY LA English DT Article DE vascular development; lymphatic development; hepatobiliary development; portal sinus; embryo; mouse; atlas ID ARYL-HYDROCARBON RECEPTOR; ENDOTHELIAL GROWTH-FACTOR; INTRAHEPATIC BILE-DUCTS; LYMPHATIC-SYSTEM; LIVER ORGANOGENESIS; ALAGILLE-SYNDROME; BILIARY-TRACT; AH RECEPTOR; HUMAN FETUS; ADRENOMEDULLIN AB A critical event in embryo development is the proper formation of the vascular system, of which the hepatobiliary system plays a pivotal role. This has led researchers to use transgenic mice to identify the critical steps involved in developmental disorders associated with the hepatobiliary vascular system. Vascular development is dependent upon normal vasculogenesis, angiogenesis, and the transformation of vessels into their adult counterparts. Any alteration in vascular development has the potential to cause deformities or embryonic death. Numerous publications describe specific stages of vascular development relating to various organs, but a single resource detailing the stage-by-stage development of the vasculature pertaining to the hepatobiliary system has not been available. This comprehensive histology atlas provides hematoxylin & eosin and immunohistochemical-stained sections of the developing mouse blood and lymphatic vasculature with emphasis on the hepatobiliary system between embryonic days (E) 11.5-18.5 and the early postnatal period. Additionally, this atlas includes a 3-dimensional video representation of the E18.5 mouse venous vasculature. One of the most noteworthy findings of this atlas is the identification of the portal sinus within the mouse, which has been erroneously misinterpreted as the ductus venosus in previous publications. Although the primary purpose of this atlas is to identify normal hepatobiliary vascular development, potential embryonic abnormalities are also described. C1 [Swartley, Olivia M.; Cullen, John M.] N Carolina State Univ, Coll Vet Med, Raleigh, NC USA. [Foley, Julie F.; Elmore, Susan A.] NIEHS, Cellular & Mol Pathol Branch, Natl Toxicol Program, NIH, POB 12233, Res Triangle Pk, NC 27709 USA. [Livingston, David P., III] USDA, Washington, DC 20250 USA. [Livingston, David P., III] N Carolina State Univ, Raleigh, NC 27695 USA. RP Elmore, SA (reprint author), NIEHS, POB 12233, Res Triangle Pk, NC 27709 USA. EM elmore@niehs.nih.gov FU Intramural Research Program of the National Institutes of Health (NIH), National Institute of Environmental Health Sciences (NIEHS) FX The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This research was supported (in part) by the Intramural Research Program of the National Institutes of Health (NIH), National Institute of Environmental Health Sciences (NIEHS). NR 58 TC 1 Z9 1 U1 1 U2 4 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0192-6233 EI 1533-1601 J9 TOXICOL PATHOL JI Toxicol. Pathol. PD JUL PY 2016 VL 44 IS 5 BP 705 EP 725 DI 10.1177/0192623316630836 PG 21 WC Pathology; Toxicology SC Pathology; Toxicology GA DQ4LH UT WOS:000379174900008 PM 26961180 ER PT J AU Valles, SM Strong, CA Callcott, AMA AF Valles, Steven M. Strong, Charles A. Callcott, Anne-Marie A. TI Development of a lateral flow immunoassay for rapid field detection of the red imported fire ant, Solenopsis invicta (Hymenoptera: Formicidae) SO ANALYTICAL AND BIOANALYTICAL CHEMISTRY LA English DT Article DE Solenopsis invicta; Solenopsis richteri; Fire ant; Venom; Antibodies; Lateral flow immunoassay ID AMINO-ACID-SEQUENCES; SOL-I-II; VENOM; POPULATIONS; ALLERGEN; FLORIDA AB The red imported fire ant, Solenopsis invicta, is an aggressive, highly invasive pest ant species from South America that has been introduced into North America, Asia, and Australia. Quarantine efforts have been imposed in the USA to minimize further spread of the ant. To aid the quarantine efforts, there remains an acute need for a rapid, field portable method for the identification of these ants. In this report, we describe two novel monoclonal antibodies that specifically bind the S. invicta venom protein 2 produced by S. invicta. Using these monoclonal antibodies we developed a lateral flow immunoassay that provides a rapid and portable method for the identification of S. invicta ants. The lateral flow immunoassay was validated against purified S. invicta venom protein 2 and 33 unique ant species (representing 15 % of the total species and 42 % of the Myrmicinae genera found in Florida), and only S. invicta and the S. invicta/richteri hybrid produced a positive result. These monoclonal antibodies were selective to S. invicta venom protein 2 and did not bind to proteins from congeners (i.e., S. geminata or S. richteri) known to produce a S. invicta venom protein 2 ortholog. This S. invicta lateral flow immunoassay provides a new tool for regulatory agencies in the USA to enforce quarantine protocols and limit the spread of this invasive ant. C1 [Valles, Steven M.; Strong, Charles A.] ARS, Ctr Med Agr & Vet Entomol, USDA, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. [Callcott, Anne-Marie A.] USDA, Anim & Plant Hlth Inspect Serv, Plant Protect & Quarantine, Biloxi, MS 39530 USA. RP Valles, SM (reprint author), ARS, Ctr Med Agr & Vet Entomol, USDA, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. EM steven.valles@ars.usda.gov NR 30 TC 0 Z9 0 U1 7 U2 9 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1618-2642 EI 1618-2650 J9 ANAL BIOANAL CHEM JI Anal. Bioanal. Chem. PD JUL PY 2016 VL 408 IS 17 BP 4693 EP 4703 DI 10.1007/s00216-016-9553-5 PG 11 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA DP8CJ UT WOS:000378725200018 PM 27108280 ER PT J AU Okonkwo, CC Azam, MM Ezeji, TC Qureshi, N AF Okonkwo, C. C. Azam, M. M. Ezeji, T. C. Qureshi, N. TI Enhancing ethanol production from cellulosic sugars using Scheffersomyces (Pichia) stipitis SO BIOPROCESS AND BIOSYSTEMS ENGINEERING LA English DT Article DE Ethanol; CaCO3; CaCl2; Xylose; Mixed cellulosic sugars; Productivity ID FERMENTATION; XYLOSE; CALCIUM; YEASTS; CONVERSION; TOLERANCE; BACTERIA; RECYCLE; STRAINS; ACETONE AB Studies were performed on the effect of CaCO3 and CaCl2 supplementation to fermentation medium for ethanol production from xylose, glucose, or their mixtures using Scheffersomyces (Pichia) stipitis. Both of these chemicals were found to improve maximum ethanol concentration and ethanol productivity. Use of xylose alone resulted in the production of 20.68 +/- 0.44 g L-1 ethanol with a productivity of 0.17 +/- 0.00 g L-1 h(-1), while xylose plus 3 g L-1 CaCO3 resulted in the production of 24.68 +/- 0.75 g L-1 ethanol with a productivity of 0.21 +/- 0.01 g L-1 h(-1). Use of xylose plus glucose in combination with 3 g L-1 CaCO3 resulted in the production of 47.37 +/- 0.55 g L-1 ethanol (aerobic culture), thus resulting in an ethanol productivity of 0.39 +/- 0.00 g L-1 h(-1). These values are 229 % of that achieved in xylose medium. Supplementation of xylose and glucose medium with 0.40 g L-1 CaCl2 resulted in the production of 44.84 +/- 0.28 g L-1 ethanol with a productivity of 0.37 +/- 0.02 g L-1 h(-1). Use of glucose plus 3 g L-1 CaCO3 resulted in the production of 57.39 +/- 1.41 g L-1 ethanol under micro-aerophilic conditions. These results indicate that supplementation of cellulosic sugars in the fermentation mediumwith CaCO3 and CaCl2 would improve economics of ethanol production from agricultural residues. C1 [Okonkwo, C. C.; Ezeji, T. C.] Ohio State Univ, Dept Anim Sci, 305 Gerlaugh Hall,1680 Madison Ave, Wooster, OH 44691 USA. [Okonkwo, C. C.; Ezeji, T. C.] Ohio State Univ, Ohio State Agr Res & Dev Ctr, 305 Gerlaugh Hall,1680 Madison Ave, Wooster, OH 44691 USA. [Azam, M. M.] Indian Inst Rice Res, Crop Improvement Sect, Plant Breeding Unit, Hyderabad 500030, TS, India. [Qureshi, N.] ARS, Bioenergy Res Unit, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. RP Ezeji, TC (reprint author), Ohio State Univ, Dept Anim Sci, 305 Gerlaugh Hall,1680 Madison Ave, Wooster, OH 44691 USA.; Ezeji, TC (reprint author), Ohio State Univ, Ohio State Agr Res & Dev Ctr, 305 Gerlaugh Hall,1680 Madison Ave, Wooster, OH 44691 USA.; Qureshi, N (reprint author), ARS, Bioenergy Res Unit, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Ezeji.1@osu.edu; Nasib.Qureshi@ars.usda.gov RI Ezeji, Thaddeus/D-7171-2015 OI Ezeji, Thaddeus/0000-0002-8384-895X FU Ohio Agricultural Research and Development Center (OARDC); Hatch grant [OHO01333] FX Additional financial support was from the Ohio Agricultural Research and Development Center (OARDC), and the Hatch grant (Project No. OHO01333). NR 24 TC 0 Z9 0 U1 3 U2 4 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1615-7591 EI 1615-7605 J9 BIOPROC BIOSYST ENG JI Bioprocess. Biosyst. Eng. PD JUL PY 2016 VL 39 IS 7 BP 1023 EP 1032 DI 10.1007/s00449-016-1580-2 PG 10 WC Biotechnology & Applied Microbiology; Engineering, Chemical SC Biotechnology & Applied Microbiology; Engineering GA DP5VQ UT WOS:000378566700001 PM 26966011 ER PT J AU McRoberts, RE Vibrans, AC Sannier, C Naesset, E Hansen, MC Walters, BF Lingner, DV AF McRoberts, Ronald E. Vibrans, Alexander C. Sannier, Christophe Naesset, Erik Hansen, Matthew C. Walters, Brian F. Lingner, Debora V. TI Methods for evaluating the utilities of local and global maps for increasing the precision of estimates of subtropical forest area SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE logistic regression model; inference; Santa Catarina; Brazil ID IMAGE-BASED MAPS; STRATIFIED ESTIMATION; LAND-COVER; ACCURACY ASSESSMENT; SATELLITE IMAGERY; INVENTORY; INFERENCE; VARIANCE AB For a study area in the Brazilian state of Santa Catarina, the utilities of local and global forest maps in combination with poststratified and model-assisted estimators for increasing the precision of estimates of forest area were compared. Auxiliary information was in the form of local maps, the recent Global Forest Change map, and combinations of these maps. The poststratified estimators produced estimates of greater precision than the model-assisted regression estimators for maps of categorical variables, but the model assisted estimators produced estimates of greater precision for maps of continuous variables. The Global Forest Change map was the least accurate of all the maps, but it produced estimates of forest area that were similar to those for the other maps and that were more precise than if the map had not been used. Thus, the Global Forest Change map may be an attractive option if local maps are not available or cannot be constructed. The primary contributions of the study are two-fold. First, this is one of the first case studies that rigorously assess the utility of global maps for national estimation. After accumulation of a few more such studies, broader generalizations should be forthcoming. Second, a statistical basis is provided for the previously unexplained greater precision for poststratified estimators than for model-assisted estimators. C1 [McRoberts, Ronald E.; Walters, Brian F.] US Forest Serv, No Res Stn, St Paul, MN USA. [Vibrans, Alexander C.; Lingner, Debora V.] Univ Reg Blumenau, Blumenau, SC, Brazil. [Sannier, Christophe] Syst Informat Reference Spatiale, Villeneuve Dascq, France. [Naesset, Erik] Norwegian Univ Life Sci, Dept Ecol & Nat Resource Management, As, Norway. [Hansen, Matthew C.] Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. RP McRoberts, RE (reprint author), US Forest Serv, No Res Stn, St Paul, MN USA. EM rmcroberts@fs.fed.us FU Fundacao de Amparo a Pesquisa e Inovacao de Santa Catarina (FAPESC); Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) FX The authors thank Fundacao de Amparo a Pesquisa e Inovacao de Santa Catarina (FAPESC) for supporting the IFFSC and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) for the research grant awarded to the second author. The authors also thank Stephen V. Stehman, State University of New York, for discussions related to comparisons of the PSTR and model-assisted estimators. NR 41 TC 1 Z9 1 U1 2 U2 7 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD JUL PY 2016 VL 46 IS 7 BP 924 EP 932 DI 10.1139/cjfr-2016-0064 PG 9 WC Forestry SC Forestry GA DP8YL UT WOS:000378783800004 ER PT J AU Pontius, J Halman, JM Schaberg, PG AF Pontius, Jennifer Halman, Joshua M. Schaberg, Paul G. TI Seventy years of forest growth and community dynamics in an undisturbed northern hardwood forest SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE competition; regeneration; succession; forest inventory; forest dynamics; Bartlett Experimental Forest ID NORTHEASTERN UNITED-STATES; BEECH BARK DISEASE; SUGAR MAPLE; RED SPRUCE; CLIMATE-CHANGE; ACIDIC DEPOSITION; NEW-HAMPSHIRE; NEW-YORK; CARBON SEQUESTRATION; SOIL ACIDIFICATION AB Long-term forest inventories provide a unique opportunity to quantify changes in forest structure and evaluate how changes compare with current stand development models. An examination of a 70 year record at the Bartlett Experimental Forest, New Hampshire, indicated that although species abundances have primarily changed as expected under natural succession, some unexpected results were also detected. This included a significant decline in sugar maple (Acer saccharum Marsh.) abundance driven by reduced regeneration and increases in red spruce (Picea rubens Sarg.) at the expense of sympatric balsam fir (Abies balsamea (L.) Mill.) and hardwoods at upper elevations. In contrast with accepted stand development models, biomass continues to accrue on these mid-to late-successional forests. Importantly, biomass accumulated at even greater rates in recent decades compared with historical norms. These results support evidence that the anthropogenic influences of a changing climate and the legacy of acid deposition may be altering stand dynamics in northeastern forests. C1 [Pontius, Jennifer] Univ Vermont, Rubenstein Sch Environm & Nat Resources, 81 Carrigan Dr, Burlington, VT 05405 USA. [Pontius, Jennifer; Schaberg, Paul G.] US Forest Serv, USDA, No Res Stn, 81 Carrigan Dr, Burlington, VT 05405 USA. [Halman, Joshua M.] Vermont Dept Forests Pk & Recreat, 111 West St, Essex Jct, VT 05452 USA. RP Pontius, J (reprint author), Univ Vermont, Rubenstein Sch Environm & Nat Resources, 81 Carrigan Dr, Burlington, VT 05405 USA. EM jennifer.pontius@uvm.edu FU USDA McIntire-Stennis Program through the wealth of the BEF long-term forest inventory FX This work would not have been possible without the dedicated work of many scientists and technicians at the USDA Forest Service Northern Research Station who meticulously collected, maintained, and shared the long-term inventory data at the BEF. We especially thank Marie Louise Smith, William Leak, Anthony D'Amato, and Richard Hallett for sharing their knowledge of the forest and offering improvements to the manuscript. Preliminary analyses and investigations by Amy Mietkiewicz and students of the University of Vermont Applied Environmental Statistics class were instrumental in directing this investigation. We are grateful for the funding provided by the USDA McIntire-Stennis Program, which allowed the authors time and support in sifting through the wealth of the BEF long-term forest inventory. NR 60 TC 0 Z9 0 U1 16 U2 25 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD JUL PY 2016 VL 46 IS 7 BP 959 EP 967 DI 10.1139/cjfr-2015-0304 PG 9 WC Forestry SC Forestry GA DP8YL UT WOS:000378783800008 ER PT J AU Penaluna, BE Dunham, JB Noakes, DLG AF Penaluna, Brooke E. Dunham, Jason B. Noakes, David L. G. TI Instream cover and shade mediate avian predation on trout in semi-natural streams SO ECOLOGY OF FRESHWATER FISH LA English DT Article DE trout survival; refuge; piscivory; body size; low flow; experiment ID SALMON ONCORHYNCHUS-KISUTCH; AGE-0 RAINBOW-TROUT; PRACTICAL GUIDE; DEPTH; RISK; HABITAT; GROWTH; BIRDS; SIZE; SURVIVAL AB Piscivory by birds can be significant, particularly on fish in small streams and during seasonal low flow when available cover from predators can be limited. Yet, how varying amounts of cover may change the extent of predation mortality from avian predators on fish is not clear. We evaluated size-selective survival of coastal cutthroat trout (Oncorhynchus clarkii clarkii) in replicated semi-natural stream sections. These sections provided high (0.01m(2) of cover per m(2) of stream) or low (0.002m(2) of cover per m(2) of stream) levels of instream cover available to trout and were closed to emigration. Each fish was individually tagged, allowing us to track retention of individuals during the course of the 36-day experiment, which we attributed to survival from predators, because fish had no other way to leave the streams. Although other avian predators may have been active in our system and not detected, the only predator observed was the belted kingfisher Megaceryle alcyon, which is known to prey heavily on fish. In both treatments, trout >20.4cm were not preyed upon indicating an increased ability to prey upon on smaller individuals. Increased availability of cover improved survival of trout by 12% in high relative to low cover stream sections. Trout also survived better in stream sections with greater shade, a factor we could not control in our system. Collectively, these findings indicate that instream cover and shade from avian predators can play an important role in driving survival of fish in small streams or during periods of low flow. C1 [Penaluna, Brooke E.] US Forest Serv, USDA, Pacific NW Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. [Penaluna, Brooke E.; Noakes, David L. G.] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Dunham, Jason B.] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Corvallis Res Grp, Corvallis, OR USA. [Noakes, David L. G.] Oregon State Univ, Oregon Hatchery Res Ctr, Alsea, OR USA. RP Penaluna, BE (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM brooke.penaluna@oregonstate.edu FU Environmental Protection Agency STAR grant; J Frances Allen scholarship from American Fisheries Society; U.S. Geological Survey; Watersheds Research Cooperative; Graduate School at Oregon State University; Department of Fisheries and Wildlife at Oregon State University FX BEP was funded by an Environmental Protection Agency STAR grant, a J Frances Allen scholarship from American Fisheries Society, a grant from the U.S. Geological Survey to Oregon State University, the Watersheds Research Cooperative and various scholarships through the Graduate School and the Department of Fisheries and Wildlife at Oregon State University. We thank Ryan Couture, Joseph O'Neil and Joyce Mahr at the Oregon Hatchery Research Centre. Dave Leer, Doug Bateman, Steve Clark, Ivan Arismendi and others helped during the experiment. Michael Young, Aaron Berger, Dave Wiens, Andrew Hafs and the Editor, Javier Lobon-Cervia, improved this manuscript. Pat Cunningham provided statistical advice. Kathryn Ronnenberg helped with graphical illustrations. Fish collections were authorised by Oregon Department of Fish and Wildlife scientific taking permit #13881 and by Oregon State University Institutional Animal Care and Use Committee proposal #3910. The use of trade or firm names here is for reader information only and does not constitute endorsement of any product or service by the US Government. NR 31 TC 1 Z9 1 U1 11 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0906-6691 EI 1600-0633 J9 ECOL FRESHW FISH JI Ecol. Freshw. Fish PD JUL PY 2016 VL 25 IS 3 BP 405 EP 411 DI 10.1111/eff.12221 PG 7 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA DP6RD UT WOS:000378625100006 ER PT J AU Ebel, ED Williams, MS Cole, D Travis, CC Klontz, KC Golden, NJ Hoekstra, RM AF Ebel, Eric D. Williams, Michael S. Cole, Dana Travis, Curtis C. Klontz, Karl C. Golden, Neal J. Hoekstra, Robert M. TI Comparing Characteristics of Sporadic and Outbreak-Associated Foodborne Illnesses, United States, 2004-2011 SO EMERGING INFECTIOUS DISEASES LA English DT Article ID SURVEILLANCE NETWORK FOODNET; DISEASE OUTBREAKS; SITES AB Outbreak data have been used to estimate the proportion of illnesses attributable to different foods. Applying outbreak based attribution estimates to nonoutbreak foodborne illnesses requires an assumption of similar exposure pathways for outbreak and sporadic illnesses. This assumption cannot be tested, but other comparisons can assess its veracity. Our study compares demographic, clinical, temporal, and geographic characteristics of outbreak and sporadic illnesses from Campylobacter, Escherichia colt 0157, Listeria, and Salmonella bacteria ascertained by the Foodborne Diseases Active Surveillance Network (FoodNet). Differences among FoodNet sites in outbreak and sporadic illnesses might reflect differences in surveillance practices. For Campylobacter, Listeria, and Escherichia colt 0157, outbreak and sporadic illnesses are similar for severity, sex, and age. For Salmonella, outbreak and sporadic illnesses are similar for severity and sex. Nevertheless, the percentage of outbreak illnesses in the youngest age category was lower. Therefore, we do not reject the assumption that outbreak and sporadic illnesses are similar. C1 [Ebel, Eric D.; Williams, Michael S.; Golden, Neal J.] US Dept Agr Dist Columbia, Washington, DC USA. [Cole, Dana; Hoekstra, Robert M.] Ctr Dis Control & Prevent, Atlanta, GA USA. [Travis, Curtis C.] Leidos Inc, Reston, VA USA. [Klontz, Karl C.] Food & Drug Adm, College Pk, MD USA. RP Williams, MS (reprint author), Food Safety & Inspect Serv, Risk Assessment & Analyt Div, Off Publ Hlth Sci, USDA, 2150 Ctr Ave,Bldg D, Ft Collins, CO 80526 USA. EM mike.williams@fsis.usda.gov NR 16 TC 0 Z9 0 U1 3 U2 8 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1080-6040 EI 1080-6059 J9 EMERG INFECT DIS JI Emerg. Infect. Dis PD JUL PY 2016 VL 22 IS 7 BP 1193 EP 1200 DI 10.3201/eid2207.150833 PG 8 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA DP5UR UT WOS:000378563900007 PM 27314510 ER PT J AU Montiel, N Buckley, A Guo, B Kulshreshtha, V VanGeelen, A Hoang, H Rademacher, C Yoon, KJ Lager, K AF Montiel, Nestor Buckley, Alexandra Guo, Baoqing Kulshreshtha, Vikas VanGeelen, Albert Hoang, Hai Rademacher, Christopher Yoon, Kyoung-Jin Lager, Kelly TI Vesicular Disease in 9-Week-Old Pigs Experimentally Infected with Senecavirus A SO EMERGING INFECTIOUS DISEASES LA English DT Article ID MOUTH-DISEASE; COMPLETE GENOME AB Senecavirus A has been infrequently associated with vesicular disease in swine since 1988. However, clinical disease has not been reproduced after experimental infection with this virus. We report vesicular disease in 9-week-old pigs after Sencavirus A infection by the intranasal route under experimental conditions. C1 [Montiel, Nestor; Buckley, Alexandra; Kulshreshtha, Vikas; VanGeelen, Albert; Lager, Kelly] USDA ARS, 1920 Dayton Ave, Ames, IA 50010 USA. [Guo, Baoqing; Hoang, Hai; Rademacher, Christopher; Yoon, Kyoung-Jin] Iowa State Univ, Coll Vet Med, Ames, IA USA. RP Lager, K (reprint author), USDA ARS, Natl Anim Dis Ctr, Virus Prion Res Unit, 1920 Dayton Ave, Ames, IA 50010 USA. EM kelly.lager@ars.usda.gov FU US Department of Energy (DOE); US Department of Agriculture; DOE [DE-AC05-06OR23100] FX This study was supported in part by an appointment to the Agricultural Research Service Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the US Department of Energy (DOE) and the US Department of Agriculture. ORISE is managed by Oak Ridge Associated Universities under DOE contract no. DE-AC05-06OR23100. NR 11 TC 5 Z9 5 U1 1 U2 2 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1080-6040 EI 1080-6059 J9 EMERG INFECT DIS JI Emerg. Infect. Dis PD JUL PY 2016 VL 22 IS 7 BP 1246 EP 1248 DI 10.3201/eid2207.151863 PG 3 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA DP5UR UT WOS:000378563900016 PM 27315363 ER PT J AU Jennelle, CS Carstensen, M Hildebrand, EC Cornicelli, L Wolf, P Grear, DA Ip, HS Vandalen, KK Minicucci, LA AF Jennelle, Christopher S. Carstensen, Michelle Hildebrand, Erik C. Cornicelli, Louis Wolf, Paul Grear, Daniel A. Ip, Hon S. Vandalen, Kaci K. Minicucci, Larissa A. TI Surveillance for Highly Pathogenic Avian Influenza Virus in Wild Birds during Outbreaks in Domestic Poultry, Minnesota, USA, 2015 SO EMERGING INFECTIOUS DISEASES LA English DT Article ID DUCKS AB In 2015, a major outbreak of highly pathogenic avian influenza virus (HPAIV) infection devastated poultry facilities in Minnesota, USA. To understand the potential role of wild birds, we tested 3,139 waterfowl fecal samples and 104 sick and dead birds during March 9 June 4, 2015. HPAIV was isolated from a Cooper's hawk but not from waterfowl fecal samples. C1 [Jennelle, Christopher S.] Minnesota Dept Nat Resources, Wildlife Hlth Program, Forest Lake, MN 55025 USA. [Carstensen, Michelle; Hildebrand, Erik C.; Cornicelli, Louis] Minnesota Dept Nat Resources, 5463-C W Broadway Ave, Forest Lake, MN 55025 USA. [Wolf, Paul] Wildlife Serv, USDA, St Paul, MN USA. [Grear, Daniel A.; Ip, Hon S.] US Geol Survey, Natl Wildlife Hlth Ctr, Madison, WI USA. [Vandalen, Kaci K.] Anim & Plant Hlth Inspect Serv, USDA, Ft Collins, CO USA. [Minicucci, Larissa A.] Univ Minnesota, Coll Vet Med, St Paul, MN 55108 USA. RP Jennelle, CS (reprint author), Minnesota Dept Nat Resources, 5463-C W Broadway Ave, Forest Lake, MN 55025 USA. EM christopher.jennelle@state.mn.us NR 15 TC 0 Z9 0 U1 8 U2 8 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1080-6040 EI 1080-6059 J9 EMERG INFECT DIS JI Emerg. Infect. Dis PD JUL PY 2016 VL 22 IS 7 BP 1278 EP 1282 DI 10.3201/eid2207.152032 PG 5 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA DP5UR UT WOS:000378563900025 PM 27064759 ER PT J AU Lee, DH Bahl, J Torchetti, MK Killian, ML Ip, HS DeLiberto, TJ Swayne, DE AF Lee, Dong-Hun Bahl, Justin Torchetti, Mia Kim Killian, Mary Lea Ip, Hon S. DeLiberto, Thomas J. Swayne, David E. TI Highly Pathogenic Avian Influenza Viruses and Generation of Novel Reassortants, United States, 2014-2015 SO EMERGING INFECTIOUS DISEASES LA English DT Article ID MIGRATORY BIRDS; DUCKS; H5N8 AB Asian highly pathogenic avian influenza A(H5N8) viruses spread into North America in 2014 during autumn bird migration. Complete genome sequencing and phylogenetic analysis of 32 H5 viruses identified novel H5N1, H5N2, and H5N8 viruses that emerged in late 2014 through reassortment with North American low-pathogenicity avian influenza viruses. C1 [Lee, Dong-Hun; Swayne, David E.] USDA ARS, Athens, GA 30613 USA. [Bahl, Justin] Univ Texas Houston, Sch Publ Hlth, Houston, TX USA. [Torchetti, Mia Kim; Killian, Mary Lea] Anim & Plant Hlth Inspect Serv, USDA, Ames, IA USA. [Ip, Hon S.] US Geol Survey, US Dept Interior, Madison, WI USA. [DeLiberto, Thomas J.] Anim & Plant Hlth Inspect Serv, USDA, Ft Collins, CO USA. RP Swayne, DE (reprint author), ARS, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. EM david.swayne@ars.usda.gov NR 15 TC 5 Z9 5 U1 6 U2 10 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1080-6040 EI 1080-6059 J9 EMERG INFECT DIS JI Emerg. Infect. Dis PD JUL PY 2016 VL 22 IS 7 BP 1283 EP 1285 DI 10.3201/eid2207.160048 PG 3 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA DP5UR UT WOS:000378563900026 PM 27314845 ER PT J AU Jaradat, AA AF Jaradat, Abdullah A. TI Evolution of Cuphea PSR23 under cultivation SO EUPHYTICA LA English DT Article DE Oilseed crop; Domestication; Phenotypic integration; Multivariate analysis ID COMMON PRINCIPAL COMPONENTS; SELECTION; TRAITS; YIELD AB A series of experiments were carried out under controlled environments and field conditions (2002-2008) to evaluate populations of the potential oilseed crop Cuphea PSR23 for indicators of evolution under cultivation and to assess its level of domestication in comparison with its wild parents. Cuphea PSR23, a selection with reduced seed shattering, resulted from a cross between two wild species (C. viscosissima and C. lanceolata). Phenotypic traits, singly or in combinations, and nutrient ratios measured on plants and seed of all three species were subjected to a set of multivariate statistical analyses procedures to identify and assess indicators of phenotypic divergence of Cuphea PSR23 from its wild parents over several planting-harvesting-seed selection cycles. Results of this assessment suggested that Cuphea PSR23 is phenotypically distinct from its wild parents (with 84.9 % correct classification in canonical discrimination). Compared to its wild parents, Cuphea PSR23 displayed stronger relationships between plant- and seed-based traits with seed yield; exhibited enhanced multivariate relationships between yield components (expressed as principal components and path analysis coefficients); and had larger oil content stored in larger seeds. However, this phenotypic divergence did not fully separate Cuphea PSR23 from its wild parents as indicated by the level of multivariate trait integration, a few shared principal components, and incomplete discrimination. C1 [Jaradat, Abdullah A.] Univ Minnesota, USDA, ARS, 803 Iowa Ave, Morris, MN 56267 USA. [Jaradat, Abdullah A.] Univ Minnesota, Dept Agron & Plant Genet, 803 Iowa Ave, Morris, MN 56267 USA. RP Jaradat, AA (reprint author), Univ Minnesota, USDA, ARS, 803 Iowa Ave, Morris, MN 56267 USA.; Jaradat, AA (reprint author), Univ Minnesota, Dept Agron & Plant Genet, 803 Iowa Ave, Morris, MN 56267 USA. EM abdullah.jaradat@ars.usda.gov NR 30 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD JUL PY 2016 VL 210 IS 1 BP 41 EP 55 DI 10.1007/s10681-016-1694-2 PG 15 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA DQ0KO UT WOS:000378888200004 ER PT J AU Clark, JS Vose, JM Luce, CH AF Clark, James S. Vose, James M. Luce, Charles H. TI Forest drought as an emerging research priority SO GLOBAL CHANGE BIOLOGY LA English DT Editorial Material DE National Drought Assessment C1 [Clark, James S.] Duke Univ, Nicholas Sch Environm, Durham, NC 27706 USA. [Vose, James M.] US Forest Serv, USDA, Southern Res Stn, Washington, DC 20250 USA. [Luce, Charles H.] US Forest Serv, USDA, Rocky Mt Res Stn, 322 E Front St, Boise, ID 83712 USA. RP Luce, CH (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, 322 E Front St, Boise, ID 83712 USA. EM cluce@fs.fed.us RI Luce, Charles/A-9267-2008 OI Luce, Charles/0000-0002-6938-9662 NR 1 TC 1 Z9 1 U1 2 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD JUL PY 2016 VL 22 IS 7 BP 2317 EP 2317 DI 10.1111/gcb.13252 PG 1 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DP8BD UT WOS:000378722000003 PM 27282321 ER PT J AU Schlesinger, WH Dietze, MC Jackson, RB Phillips, RP Rhoades, CC Rustad, LE Vose, JM AF Schlesinger, William H. Dietze, Michael C. Jackson, Robert B. Phillips, Richard P. Rhoades, Charles C. Rustad, Lindsey E. Vose, James M. TI Forest biogeochemistry in response to drought SO GLOBAL CHANGE BIOLOGY LA English DT Review DE biogeochemistry; carbon cycle; fire; forest management; insect attack; soil biogeochemistry ID MOUNTAIN PINE-BEETLE; WESTERN UNITED-STATES; CANADA BOREAL FORESTS; STAND-REPLACING FIRE; CLIMATE-CHANGE; NORWAY SPRUCE; TREE GROWTH; SOIL RESPIRATION; PRESCRIBED FIRE; ORGANIC-MATTER AB Trees alter their use and allocation of nutrients in response to drought, and changes in soil nutrient cycling and trace gas flux (N2O and CH4) are observed when experimental drought is imposed on forests. In extreme droughts, trees are increasingly susceptible to attack by pests and pathogens, which can lead to major changes in nutrient flux to the soil. Extreme droughts often lead to more common and more intense forest fires, causing dramatic changes in the nutrient storage and loss from forest ecosystems. Changes in the future manifestation of drought will affect carbon uptake and storage in forests, leading to feedbacks to the Earth's climate system. We must improve the recognition of drought in nature, our ability to manage our forests in the face of drought, and the parameterization of drought in earth system models for improved predictions of carbon uptake and storage in the world's forests. C1 [Schlesinger, William H.] Cary Inst Ecosyst Studies, Box AB, Millbrook, NY 12545 USA. [Dietze, Michael C.] Boston Univ, Dept Biol, 5 Cummington Mall, Boston, MA 02215 USA. [Jackson, Robert B.] Stanford Univ, Dept Earth Syst Sci, Y2E2 Bldg,379B, Stanford, CA 94305 USA. [Phillips, Richard P.] Indiana Univ, Dept Biol, 1 E 3rd St, Bloomington, IN 47405 USA. [Rhoades, Charles C.] US Forest Serv, USDA, Rocky Mt Res Stn, 240 West Prospect Rd, Ft Collins, CO 80526 USA. [Rustad, Lindsey E.] US Forest Serv, USDA, No Res Stn, 271 Mast Rd, Durham, NH 03824 USA. [Vose, James M.] N Carolina State Univ, USDA, US Forest Serv, Southern Res Stn, Campus Box 8008, Raleigh, NC 27695 USA. RP Schlesinger, WH (reprint author), Cary Inst Ecosyst Studies, Box AB, Millbrook, NY 12545 USA. EM schlesingerw@caryinstitute.org FU Cary Institute of Ecosystem Studies; USDA-AFRI [2012-00857]; NSF [1354732, 1241894]; DOE FX Supported by the Cary Institute of Ecosystem Studies. RBJ acknowledges additional grant support from USDA-AFRI (#2012-00857), RPP acknowledges support from NSF Ecosystem Studies Program Award #1354732 and from DOE via the Ameriflux Management Program, and MCD acknowledges support from NSF Macrosystems grant 1241894. NR 164 TC 2 Z9 2 U1 38 U2 74 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD JUL PY 2016 VL 22 IS 7 BP 2318 EP 2328 DI 10.1111/gcb.13105 PG 11 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DP8BD UT WOS:000378722000004 PM 26403995 ER PT J AU Clark, JS Iverson, L Woodall, CW Allen, CD Bell, DM Bragg, DC D'Amato, AW Davis, FW Hersh, MH Ibanez, I Jackson, ST Matthews, S Pederson, N Peters, M Schwartz, MW Waring, KM Zimmermann, NE AF Clark, James S. Iverson, Louis Woodall, Christopher W. Allen, Craig D. Bell, David M. Bragg, Don C. D'Amato, Anthony W. Davis, Frank W. Hersh, Michelle H. Ibanez, Ines Jackson, Stephen T. Matthews, Stephen Pederson, Neil Peters, Matthew Schwartz, Mark W. Waring, Kristen M. Zimmermann, Niklaus E. TI The impacts of increasing drought on forest dynamics, structure, and biodiversity in the United States SO GLOBAL CHANGE BIOLOGY LA English DT Review DE climate change; drought; forest dieback; forest management ID PONDEROSA PINE FORESTS; EASTERN NORTH-AMERICA; INTERANNUAL CLIMATE VARIABILITY; SPECIES DISTRIBUTION MODELS; PINYON-JUNIPER WOODLANDS; GLOBAL VEGETATION MODEL; MIXED HARDWOOD FOREST; GREAT-LAKES REGION; HIGH-SEVERITY FIRE; NEW-YORK-STATE AB We synthesize insights from current understanding of drought impacts at stand-to-biogeographic scales, including management options, and we identify challenges to be addressed with new research. Large stand-level shifts underway in western forests already are showing the importance of interactions involving drought, insects, and fire. Diebacks, changes in composition and structure, and shifting range limits are widely observed. In the eastern US, the effects of increasing drought are becoming better understood at the level of individual trees, but this knowledge cannot yet be confidently translated to predictions of changing structure and diversity of forest stands. While eastern forests have not experienced the types of changes seen in western forests in recent decades, they too are vulnerable to drought and could experience significant changes with increased severity, frequency, or duration in drought. Throughout the continental United States, the combination of projected large climate-induced shifts in suitable habitat from modeling studies and limited potential for the rapid migration of tree populations suggests that changing tree and forest biogeography could substantially lag habitat shifts already underway. Forest management practices can partially ameliorate drought impacts through reductions in stand density, selection of drought-tolerant species and genotypes, artificial regeneration, and the development of multistructured stands. However, silvicultural treatments also could exacerbate drought impacts unless implemented with careful attention to site and stand characteristics. Gaps in our understanding should motivate new research on the effects of interactions involving climate and other species at the stand scale and how interactions and multiple responses are represented in models. This assessment indicates that, without a stronger empirical basis for drought impacts at the stand scale, more complex models may provide limited guidance. C1 [Clark, James S.] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Iverson, Louis] US Forest Serv, No Res Stn, 359 Main Rd, Delaware, OH 43015 USA. [Woodall, Christopher W.] US Forest Serv, 1992 Folwell Ave, St Paul, MN 55108 USA. [Allen, Craig D.] US Geol Survey, Ft Collins Sci Ctr, Jemez Mt Field Stn, Los Alamos, NM 87544 USA. [Bell, David M.] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Bragg, Don C.] US Forest Serv, Southern Res Stn, Monticello, AR 71656 USA. [D'Amato, Anthony W.] Univ Vermont, Rubenstein Sch Environm & Nat Resources, 04E Aiken Ctr,81 Carrigan Dr, Burlington, VT 05405 USA. [Davis, Frank W.] Univ Calif Santa Barbara, Bren Sch Environm Sci & Management, Santa Barbara, CA 93106 USA. [Hersh, Michelle H.] Sarah Lawrence Coll, Dept Biol, New York, NY 10708 USA. [Ibanez, Ines] Univ Michigan, Sch Nat Resources & Environm, 2546 Dana Bldg, Ann Arbor, MI 48109 USA. [Jackson, Stephen T.] US Geol Survey, Southwest Climate Sci Ctr, 1064 E Lowell St,POB 210137, Tucson, AZ 85721 USA. [Jackson, Stephen T.] Univ Arizona, Dept Geosci, 1064 E Lowell St,POB 210137, Tucson, AZ 85721 USA. [Matthews, Stephen] Ohio State Univ, Sch Environm & Nat Resources, Columbus, OH 43210 USA. [Pederson, Neil] Harvard Forest, Petersham, MA 01366 USA. [Peters, Matthew] US Forest Serv, No Res Stn, Delaware, OH 43015 USA. [Schwartz, Mark W.] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 93106 USA. [Waring, Kristen M.] No Arizona Univ, Sch Forestry, Flagstaff, AZ 86001 USA. [Zimmermann, Niklaus E.] Swiss Fed Res Inst WSL, Birmensdorf, Switzerland. RP Clark, JS (reprint author), Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. EM jimclark@duke.edu RI Zimmermann, Niklaus/A-4276-2008; Schwartz, Mark/G-1066-2011; Davis, Frank/B-7010-2009; Matthews, Stephen/D-1050-2012 OI Zimmermann, Niklaus/0000-0003-3099-9604; Schwartz, Mark/0000-0002-3739-6542; Davis, Frank/0000-0002-4643-5718; FU Department of Interior Northeast Climate Science Center; [NSF-EF-1550911] FX For helpful comments, we thank David Ackerly and three anonymous reviewers. Support is acknowledged from NSF-EF-1550911 (to JSC) and the Department of Interior Northeast Climate Science Center (to AWD). NR 302 TC 16 Z9 16 U1 64 U2 114 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD JUL PY 2016 VL 22 IS 7 BP 2329 EP 2352 DI 10.1111/gcb.13160 PG 24 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DP8BD UT WOS:000378722000005 PM 26898361 ER PT J AU Littell, JS Peterson, DL Riley, KL Liu, YQ Luce, CH AF Littell, Jeremy S. Peterson, David L. Riley, Karin L. Liu, Yongquiang Luce, Charles H. TI A review of the relationships between drought and forest fire in the United States SO GLOBAL CHANGE BIOLOGY LA English DT Review DE climate change; climate variability; drought; ecological drought; fire; water balance ID PINYON-JUNIPER WOODLANDS; SPRUCE BEETLE OUTBREAK; WESTERN NORTH-AMERICA; CLIMATE-CHANGE; PACIFIC-NORTHWEST; VEGETATION DISTRIBUTION; KENAI PENINSULA; DANGER INDEXES; INTERIOR WEST; SIERRA-NEVADA AB The historical and presettlement relationships between drought and wildfire are well documented in North America, with forest fire occurrence and area clearly increasing in response to drought. There is also evidence that drought interacts with other controls (forest productivity, topography, fire weather, management activities) to affect fire intensity, severity, extent, and frequency. Fire regime characteristics arise across many individual fires at a variety of spatial and temporal scales, so both weather and climate - including short- and long-term droughts - are important and influence several, but not all, aspects of fire regimes. We review relationships between drought and fire regimes in United States forests, fire-related drought metrics and expected changes in fire risk, and implications for fire management under climate change. Collectively, this points to a conceptual model of fire on real landscapes: fire regimes, and how they change through time, are products of fuels and how other factors affect their availability (abundance, arrangement, continuity) and flammability (moisture, chemical composition). Climate, management, and land use all affect availability, flammability, and probability of ignition differently in different parts of North America. From a fire ecology perspective, the concept of drought varies with scale, application, scientific or management objective, and ecosystem. C1 [Littell, Jeremy S.] DOI Alaska Climate Sci Ctr, 4210 Univ Dr, Anchorage, AK 99508 USA. [Peterson, David L.] US Forest Serv, USDA, Pacific NW Res Stn, 400 N 34th St,Suite 201, Seattle, WA 98103 USA. [Riley, Karin L.] US Forest Serv, USDA, Rocky Mt Res Stn, 800 East Beckwith, Missoula, MT 59801 USA. [Liu, Yongquiang] US Forest Serv, USDA, Southern Res Stn, 320 Green St, Athens, GA 30602 USA. [Luce, Charles H.] US Forest Serv, USDA, Rocky Mt Res Stn, 322 East Front St,Suite 401, Boise, ID 83702 USA. RP Littell, JS (reprint author), DOI Alaska Climate Sci Ctr, 4210 Univ Dr, Anchorage, AK 99508 USA. EM jlittell@usgs.gov RI Luce, Charles/A-9267-2008 OI Luce, Charles/0000-0002-6938-9662 NR 155 TC 5 Z9 5 U1 35 U2 53 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD JUL PY 2016 VL 22 IS 7 BP 2353 EP 2369 DI 10.1111/gcb.13275 PG 17 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DP8BD UT WOS:000378722000006 PM 27090489 ER PT J AU Estiarte, M Vicca, S Penuelas, J Bahn, M Beier, C Emmett, BA Fay, PA Hanson, PJ Hasibeder, R Kigel, J Kroel-Dulay, G Larsen, KS Lellei-Kovacs, E Limousin, JM Ogaya, R Ourcival, JM Reinsch, S Sala, OE Schmidt, IK Sternberg, M Tielborger, K Tietema, A Janssens, IA AF Estiarte, Marc Vicca, Sara Penuelas, Josep Bahn, Michael Beier, Claus Emmett, Bridget A. Fay, Philip A. Hanson, Paul J. Hasibeder, Roland Kigel, Jaime Kroel-Dulay, Gyorgy Larsen, Klaus Steenberg Lellei-Kovacs, Eszter Limousin, Jean-Marc Ogaya, Roma Ourcival, Jean-Marc Reinsch, Sabine Sala, Osvaldo E. Schmidt, Inger Kappel Sternberg, Marcelo Tielboerger, Katja Tietema, Albert Janssens, Ivan A. TI Few multiyear precipitation-reduction experiments find ashift in the productivity-precipitation relationship SO GLOBAL CHANGE BIOLOGY LA English DT Article DE aboveground productivity; drought; precipitation; precipitation-reduction experiments; spatial fit; temporal fit ID CARBON-CYCLE MODELS; HOLM OAK FOREST; MANIPULATION EXPERIMENTS; CLIMATE-CHANGE; USE EFFICIENCY; TREE GROWTH; DROUGHT; VARIABILITY; GRASSLAND; MORTALITY AB Well-defined productivity-precipitation relationships of ecosystems are needed as benchmarks for the validation of land models used for future projections. The productivity-precipitation relationship may be studied in two ways: the spatial approach relates differences in productivity to those in precipitation among sites along a precipitation gradient (the spatial fit, with a steeper slope); the temporal approach relates interannual productivity changes to variation in precipitation within sites (the temporal fits, with flatter slopes). Precipitation-reduction experiments in natural ecosystems represent a complement to the fits, because they can reduce precipitation below the natural range and are thus well suited to study potential effects of climate drying. Here, we analyse the effects of dry treatments in eleven multiyear precipitation-manipulation experiments, focusing on changes in the temporal fit. We expected that structural changes in the dry treatments would occur in some experiments, thereby reducing the intercept of the temporal fit and displacing the productivity-precipitation relationship downward the spatial fit. The majority of experiments (72%) showed that dry treatments did not alter the temporal fit. This implies that current temporal fits are to be preferred over the spatial fit to benchmark land-model projections of productivity under future climate within the precipitation ranges covered by the experiments. Moreover, in two experiments, the intercept of the temporal fit unexpectedly increased due to mechanisms that reduced either water loss or nutrient loss. The expected decrease of the intercept was observed in only one experiment, and only when distinguishing between the late and the early phases of the experiment. This implies that we currently do not know at which precipitation-reduction level or at which experimental duration structural changes will start to alter ecosystem productivity. Our study highlights the need for experiments with multiple, including more extreme, dry treatments, to identify the precipitation boundaries within which the current temporal fits remain valid. C1 [Estiarte, Marc; Penuelas, Josep] CSIC, Global Ecol Unit CREAF CSIC UAB, E-08193 Cerdanyola Del Valles, Catalonia, Spain. [Estiarte, Marc; Penuelas, Josep; Ogaya, Roma] CREAF, E-08193 Barcelona, Catalonia, Spain. [Vicca, Sara; Janssens, Ivan A.] Univ Antwerp, Dept Biol, B-2610 Antwerp, Belgium. [Bahn, Michael; Hasibeder, Roland] Univ Innsbruck, Inst Ecol, Sternwarte Str 15, A-6020 Innsbruck, Austria. [Beier, Claus; Larsen, Klaus Steenberg; Schmidt, Inger Kappel] Univ Copenhagen, Dept Geosci & Nat Resource Management, Rolighedsvej 23, DK-1958 Frederiksberg C, Denmark. [Beier, Claus; Reinsch, Sabine] NIVA, Ctr Catchments & Urban Water Res, NO-0349 Oslo, Norway. [Emmett, Bridget A.] Environm Ctr Wales, Ctr Ecol & Hydrol, Bangor LL57 2UW, Gwynedd, Wales. [Fay, Philip A.] USDA ARS, 808 E Blackland Rd, Temple, TX 76502 USA. [Hanson, Paul J.] Oak Ridge Natl Lab, Climate Change Sci Inst, Oak Ridge, TN 37831 USA. [Kigel, Jaime] Hebrew Univ Jerusalem, Inst Plant Sci & Genet, Robert H Smith Fac Agr Food & Environm, IL-76100 Rehovot, Israel. [Kroel-Dulay, Gyorgy; Lellei-Kovacs, Eszter] MTA Ctr Ecol Res, Inst Ecol & Bot, H-2163 Vacratot, Hungary. [Limousin, Jean-Marc; Ourcival, Jean-Marc] Univ Montpellier 3, Univ Montpellier, CNRS, CEFE,UMR5175,EPHE, 1919 Route Mende, F-34293 Montpellier 5, France. [Sala, Osvaldo E.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Sala, Osvaldo E.] Arizona State Univ, Sch Sustainabil, Tempe, AZ 85287 USA. [Sternberg, Marcelo] Tel Aviv Univ, Fac Life Sci, Dept Mol Biol & Ecol Plants, IL-69978 Tel Aviv, Israel. [Tielboerger, Katja] Univ Tubingen, Plant Ecol Grp, Dept Biol, Morgenstelle 3, D-72076 Tubingen, Germany. [Tietema, Albert] Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, POB 94240, NL-1090 GE Amsterdam, Netherlands. RP Estiarte, M (reprint author), CSIC, Global Ecol Unit CREAF CSIC UAB, E-08193 Cerdanyola Del Valles, Catalonia, Spain.; Estiarte, M (reprint author), CREAF, E-08193 Barcelona, Catalonia, Spain. EM m.estiarte@creaf.uab.cat RI Hanson, Paul J./D-8069-2011; Vicca, Sara/I-3637-2012; Bahn, Michael/I-3536-2013; Emmett, Bridget/D-6199-2011; Janssens, Ivan/P-1331-2014; Estiarte, Marc/G-2001-2016; OI Hanson, Paul J./0000-0001-7293-3561; Vicca, Sara/0000-0001-9812-5837; Bahn, Michael/0000-0001-7482-9776; Emmett, Bridget/0000-0002-2713-4389; Janssens, Ivan/0000-0002-5705-1787; Estiarte, Marc/0000-0003-1176-8480; Larsen, Klaus Steenberg/0000-0002-1421-6182; Penuelas, Josep/0000-0002-7215-0150 FU European Community [FP7-ENV-2008-1-226701]; ESF-network CLIMMANI; COST action [5ES1308]; Spanish Government [CGL2013-48074-P]; Catalan Government [SGR 2014-274]; European Research Council [ERC-2013-SyG 610028-IMBALANCE-P]; US National Science Foundation [DEB-1235828, DEB 1354732]; USDA-NIFA [2010-65615-20632]; Israel Ministry of Science and Technology (MOST); German Ministry of Science and Education (BMBF); FP7 (INCREASE) programmes [227628]; Hungarian Scientific Research Fund [OTKA K112576, PD 115637]; Austrian Science Fund-FWF [P22214-B17]; ERA-Net BiodivERsA project REGARDS [FWF-I-1056]; U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research FX This work emerged from the Carbo-Extreme project funded by the European Community's 7th Framework Programme under grant agreement FP7-ENV-2008-1-226701 and has been supported by the ESF-network CLIMMANI and the COST action 5ES1308. ME, JP and RO were supported by the Spanish Government grants CGL2013-48074-P, the Catalan Government grant SGR 2014-274 and the European Research Council grant ERC-2013-SyG 610028-IMBALANCE-P. SV is a postdoctoral fellow of the Research Foundation - Flanders (FWO). OES acknowledges support from the US National Science Foundation DEB-1235828 and DEB 1354732. PAF acknowledges support from USDA-NIFA (2010-65615-20632). MS and JK were supported by the Israel Ministry of Science and Technology (MOST). Research by KT, MS and JK was part of the GLOWA Jordan River project, funded by the German Ministry of Science and Education (BMBF). GK-D and EL-K were supported by the FP7 (INCREASE: 227628) programmes, and by the Hungarian Scientific Research Fund (OTKA K112576 and PD 115637). MB and RH were supported by the Austrian Science Fund-FWF grant P22214-B17 and the ERA-Net BiodivERsA project REGARDS (FWF-I-1056). PJH was supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research. We thank Roberto Molowny for his advice on data treatment. AT thanks Joke Westerveld for assistance with the experiment. NR 46 TC 2 Z9 2 U1 31 U2 52 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD JUL PY 2016 VL 22 IS 7 BP 2570 EP 2581 DI 10.1111/gcb.13269 PG 12 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DP8BD UT WOS:000378722000023 PM 26946322 ER PT J AU Wang, DR Bunce, JA Tomecek, MB Gealy, D McClung, A McCouch, SR Ziska, LH AF Wang, Diane R. Bunce, James A. Tomecek, Martha B. Gealy, David McClung, Anna McCouch, Susan R. Ziska, Lewis H. TI Evidence for divergence of response in Indica, Japonica, and wild rice to high CO2 x temperature interaction SO GLOBAL CHANGE BIOLOGY LA English DT Article DE carbon dioxide; genetic diversity; plasticity; resilience; response; rice; temperature stress ID ATMOSPHERIC CARBON-DIOXIDE; QUANTITATIVE TRAIT LOCI; ORYZA-SATIVA L; ELEVATED CO2; GENOTYPIC VARIATION; AIR-TEMPERATURE; YIELD; GROWTH; ENRICHMENT; CULTIVARS AB High CO2 and high temperature have an antagonistic interaction effect on rice yield potential and present a unique challenge to adapting rice to projected future climates. Understanding how the differences in response to these two abiotic variables are partitioned across rice germplasm accessions may be key to identifying potentially useful sources of resilient alleles for adapting rice to climate change. In this study, we evaluated eleven globally diverse rice accessions under controlled conditions at two carbon dioxide concentrations (400 and 600ppm) and four temperature environments (29 degrees C day/21 degrees C night; 29 degrees C day/21 degrees C night with additional heat stress at anthesis; 34 degrees C day/26 degrees C night; and 34 degrees C day/26 degrees C night with additional heat stress at anthesis) for a suite of traits including five yield components, five growth characteristics, one phenological trait, and four photosynthesis-related measurements. Multivariate analyses of mean trait data from these eight treatments divide our rice panel into two primary groups consistent with the genetic classification of INDICA/INDICA-like and JAPONICA populations. Overall, we find that the productivity of plants grown under elevated [CO2] was more sensitive (negative response) to high temperature stress compared with that of plants grown under ambient [CO2] across this diversity panel. We report differential response to CO2 x temperature interaction for INDICA/INDICA-like and JAPONICA rice accessions and find preliminary evidence for the beneficial introduction of exotic alleles into cultivated rice genomic background. Overall, these results support the idea of using wild or currently unadapted gene pools in rice to enhance breeding efforts to secure future climate change adaptation. C1 [Wang, Diane R.; McCouch, Susan R.] Cornell Univ, Sch Integrated Plant Sci, Sect Plant Breeding & Genet, Ithaca, NY 14850 USA. [Bunce, James A.; Tomecek, Martha B.; Ziska, Lewis H.] USDA ARS, Crop Syst & Global Change Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. [Gealy, David; McClung, Anna] USDA ARS, Dale Bumpers Natl Rice Res Ctr, 2890 HWY 130 E, Stuttgart, AR 72160 USA. RP Ziska, LH (reprint author), USDA ARS, Crop Syst & Global Change Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM l.ziska@ars.usda.gov FU NSF [IOS-1026555]; USDA NIFA [2014-67003-21858]; National Science Foundation [DGE-1144153] FX We thank Daryl Baxam for expert technical assistance. We are grateful for support from NSF #IOS-1026555 and USDA NIFA #2014-67003-21858. DRW is supported by the National Science Foundation Graduate Research Fellowship under Grant #DGE-1144153. NR 33 TC 1 Z9 1 U1 14 U2 27 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD JUL PY 2016 VL 22 IS 7 BP 2620 EP 2632 DI 10.1111/gcb.13279 PG 13 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DP8BD UT WOS:000378722000027 PM 26959982 ER PT J AU Miller, JN VanLoocke, A Gomez-Casanovas, N Bernacchi, CJ AF Miller, Jesse N. VanLoocke, Andy Gomez-Casanovas, Nuria Bernacchi, Carl J. TI Candidate perennial bioenergy grasses have a higher albedo than annual row crops SO GLOBAL CHANGE BIOLOGY BIOENERGY LA English DT Article DE albedo; bioenergy; biofuel crops; corn; land-use change; miscanthus; radiative forcing; snow; soybean; switchgrass ID MISCANTHUS X GIGANTEUS; UNITED-STATES; SURFACE ALBEDO; PANICUM-VIRGATUM; REGIONAL CLIMATE; FOLIAR NITROGEN; BIOFUEL CROPS; IMPACTS; ENERGY; CARBON AB The production of perennial cellulosic feedstocks for bioenergy presents the potential to diversify regional economies and the national energy supply, while also serving as climate regulators' due to a number of biogeochemical and biogeophysical differences relative to row crops. Numerous observational and model-based approaches have investigated biogeochemical trade-offs, such as increased carbon sequestration and increased water use, associated with growing cellulosic feedstocks. A less understood aspect is the biogeophysical changes associated with the difference in albedo (), which could alter the local energy balance and cause local to regional cooling several times larger than that associated with offsetting carbon. Here, we established paired fields of Miscanthusxgiganteus (miscanthus) and Panicum virgatum (switchgrass), two of the leading perennial cellulosic feedstock candidates, and traditional annual row crops in the highly productive Corn-belt'. Our results show that miscanthus did and switchgrass did not have an overall higher than current row crops, but a strong seasonal pattern existed. Both perennials had consistently higher growing season than row crops and winter did not differ. The lack of observed differences in winter , however, masked an interaction between snow cover and species differences, with the perennial species, compared with the row crops, having a higher when snow was absent and a much lower when snow was present. Overall, these changes resulted in an average net reduction in annual absorbed energy of about 5Wm(-2) for switchgrass and about 8Wm(-2) for miscanthus relative to annual crops. Therefore, the conversion from annual row to perennial crops alters the radiative balance of the surface via changes in and could lead to regional cooling. C1 [Miller, Jesse N.; Bernacchi, Carl J.] Univ Illinois, Dept Plant Biol, 1201 W Gregory Dr,196 Madigan Lab, Urbana, IL 61801 USA. [VanLoocke, Andy] ARS, USDA, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. [Gomez-Casanovas, Nuria] Univ Illinois, Inst Genom Biol, Energy Biosci Inst, 1206 W Gregory Dr,Room 1400, Urbana, IL 61801 USA. [VanLoocke, Andy] Iowa State Univ, Dept Agron, Ames, IA USA. RP Bernacchi, CJ (reprint author), 1201 W Gregory Dr,196 ERML, Urbana, IL 61801 USA. EM bernacch@illinois.edu FU Energy Biosciences Institute, University of Illinois at Urbana-Champaign; USDA FX The authors wish to acknowledge Alex Crisel, Robert Nystrom, Aaron Letterly, Kyle Dawson, and Christina Burke for assisting in data collection and equipment maintenance and Marcelo Zeri and Ursula Ruiz-Vera in assisting with various aspects of experimental setup and data interpretation. The authors further acknowledge Gary Letterly and Ron Sloan for providing access to the Dudley Smith Farms. This study was funded by the Energy Biosciences Institute, University of Illinois at Urbana-Champaign and the USDA. NR 61 TC 3 Z9 3 U1 11 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1757-1693 EI 1757-1707 J9 GCB BIOENERGY JI GCB Bioenergy PD JUL PY 2016 VL 8 IS 4 BP 818 EP 825 DI 10.1111/gcbb.12291 PG 8 WC Agronomy; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA DP3ZJ UT WOS:000378434700010 ER PT J AU Ebanyenle, E Burton, AJ Storer, AJ Richter, DL Glaeser, JA AF Ebanyenle, Emmanuel Burton, Andrew J. Storer, Andrew J. Richter, Dana L. Glaeser, Jessie A. TI Elevated tropospheric CO2 and O-3 may not alter initial wood decomposition rate or wood-decaying fungal community composition of Northern Hardwoods SO INTERNATIONAL BIODETERIORATION & BIODEGRADATION LA English DT Article DE Elevated atmospheric CO2; Elevated atmospheric O-3; Wood decomposition; Fungal community; Northern hardwoods ID ATMOSPHERIC CO2; AVAILABILITY; ALLOCATION; GROWTH; FOREST AB We examined the effects of elevated CO2 and/or O-3 on the wood-decaying basidiomycete fungal community and wood decomposition rates at the Aspen Free-Air CO2 and O-3 Enrichment (Aspen FACE) project. Mass loss rates were determined after one year of log decomposition on the soil surface, and wood-decaying basidiomycetes were isolated from decaying wood and identified via DNA sequencing. Aspen (Populus tremuloides Michx.) and birch (Betula papyrifera Marshall) wood differed significantly in wood-decaying basidiomycete fungal communities and decomposition rate. Twelve years of site exposure to elevated CO2 and/or O-3 did not have significant effects on wood-decaying fungal communities. Growth under elevated CO2 and/or O-3 did not produce wood that differed in decay rate from that grown under ambient atmospheric conditions. Similarly, wood decay rate was not altered significantly when decomposition occurred in elevated CO2 and/or O-3 environments. Our results suggest that wood decaying fungal community composition and decomposition rates of northern hardwoods may not be directly affected by elevated tropospheric CO2 and O-3. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Ebanyenle, Emmanuel; Burton, Andrew J.; Storer, Andrew J.; Richter, Dana L.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Ecosyst Sci Ctr, 1400 Townsend Dr, Houghton, MI 49931 USA. [Glaeser, Jessie A.] US Forest Serv, Ctr Forest Mycol Res, No Res Stn, One Gifford Pinchot Dr, Madison, WI 53726 USA. RP Ebanyenle, E (reprint author), CSIR Forestry Res Inst Ghana, KNUST, UPO 63, Kumasi, Ghana. EM eebanyen@mtu.edu; ajburton@mtu.edu; storer@mtu.edu; dlrichte@mtu.edu; jglaeser@fs.fed.us FU US Department of Energy [DE-FG02-95ER62125]; US Forest Service Northern Research Station [10-JV-11242313-051] FX This study was supported by the US Department of Energy (DE-FG02-95ER62125) and the US Forest Service Northern Research Station (10-JV-11242313-051). We thank Becky Bender and Daniel Yeboah for assistance in fungal isolation and wood density measurement and Kyah Norton and John Haight for DNA isolation and fungal identification. We are grateful to the anonymous reviewers for helping in improving the manuscript. NR 24 TC 0 Z9 0 U1 4 U2 4 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0964-8305 EI 1879-0208 J9 INT BIODETER BIODEGR JI Int. Biodeterior. Biodegrad. PD JUL PY 2016 VL 111 BP 74 EP 77 DI 10.1016/j.ibiod.2016.04.026 PG 4 WC Biotechnology & Applied Microbiology; Environmental Sciences SC Biotechnology & Applied Microbiology; Environmental Sciences & Ecology GA DP4LN UT WOS:000378467500009 ER PT J AU Cheng, HN Ford, C Dowd, MK He, ZQ AF Cheng, H. N. Ford, Catrina Dowd, Michael K. He, Zhongqi TI Use of additives to enhance the properties of cottonseed protein as wood adhesives SO INTERNATIONAL JOURNAL OF ADHESION AND ADHESIVES LA English DT Article DE Cottonseed protein; Soy protein; Adhesion; Wood veneer; Protein modifiers ID IMPROVED WATER RESISTANCE; MODIFIED SOY PROTEIN; GUANIDINE-HYDROCHLORIDE; MEAL; PLASTICIZERS; COMPOSITES; PRODUCTS; STRENGTH; IMPROVE; SYSTEMS AB Soy protein is currently being used commercially as a "green" wood adhesive. Previous work in this laboratory has shown that cottonseed protein isolate, tested on maple wood veneer, produced higher adhesive strength and hot water resistance relative to soy protein. In the present study, cottonseed protein and soy protein isolates were tested on different wood types, and cottonseed protein again showed better performance relative to soy protein. Furthermore, the effects of several protein modifiers were evaluated, including amino acids, fatty acids, and other organic molecules with cationic or anionic charges. Aspartic acid, glutamic acid, acetic acid, butyric acid, and adipic acid gave improved performance when included with cottonseed protein isolate whereas no significant effect was observed on soy protein isolate. Both dry adhesive strength and hot water resistance were tested. The enhanced performance observed with these additives provides an additional incentive for the use of cottonseed protein in this application. Published by Elsevier Ltd. C1 [Cheng, H. N.; Ford, Catrina; Dowd, Michael K.; He, Zhongqi] USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. RP Cheng, HN (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM hn.cheng@ars.usda.gov NR 39 TC 1 Z9 1 U1 5 U2 6 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0143-7496 EI 1879-0127 J9 INT J ADHES ADHES JI Int. J. Adhes. Adhes. PD JUL PY 2016 VL 68 BP 156 EP 160 DI 10.1016/j.ijadhadh.2016.02.012 PG 5 WC Engineering, Chemical; Materials Science, Multidisciplinary SC Engineering; Materials Science GA DP4MQ UT WOS:000378470400018 ER PT J AU Cooper, TA Eaglen, SAE Wiggans, GR Jenko, J Huson, HJ Morrice, DR Bichard, M Luff, WGD Woolliams, JA AF Cooper, T. A. Eaglen, S. A. E. Wiggans, G. R. Jenko, J. Huson, H. J. Morrice, D. R. Bichard, M. Luff, W. G. de L. Woolliams, J. A. TI Genomic evaluation, breed identification, and population structure of Guernsey cattle in North America, Great Britain, and the Isle of Guernsey SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE Guernsey; breed determination; genomic evaluation; principal component analysis; population structure ID UNITED-STATES; DAIRY-CATTLE; FERTILITY; BULLS AB As of December 2015, 2,376 Guernsey bulls and cows had genotypes from collaboration between the United States, Canada, the United Kingdom, and the Isle of Guernsey. Of those, 439 bulls and 504 cows had traditional US evaluations, which provided sufficient data to justify investigation of the possible benefits of genomic evaluation for the Guernsey breed. Evaluation accuracy was assessed using a traditional 4-yr cutoff study. Twenty-two traits were analyzed (5 yield traits, 3 functional traits, and 14 conformation traits). Mean reliability gain over that for parent average was 16.8 percentage points across traits, which compares with 8.2. 18.5, 20.0, and 32.6 percentage points reported for Ayrshires, Brown Swiss, Jerseys, and Holsteins, respectively. Highest Guernsey reliability gains were for rump width (44.5 percentage points) and dairy form (40.5 percentage points); lowest gains were for teat length (1.9 percentage points) and rear legs (side view) (2.3 percentage points). Slight reliability losses (1.5 to 4.5 percentage points) were found for udder cleft, final score, and udder depth as well as a larger loss (13.6 percentage points) for fore udder attachment. Twenty-one single nucleotide polymorphisms were identified for Guernsey breed determination and can be used in routine genotype quality control to confirm breed and identify crossbreds. No haplotypes that affect fertility were identified from the current data set. Principal component analysis showed some divergence of US and Isle of Guernsey subpopulations. However, the overlap of US, Canadian, UK, and Isle of Guernsey subpopulations indicated the presence of gene flow, and the similarities in the subpopulations supports a common genomic evaluation system across the regions. C1 [Cooper, T. A.; Wiggans, G. R.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. [Eaglen, S. A. E.; Jenko, J.; Woolliams, J. A.] Univ Edinburgh, Roslin Inst, Royal Dick Sch Vet Studies, Easter Bush EH25 9RG, Midlothian, Scotland. [Huson, H. J.] Cornell Univ, Dept Anim Sci, Ithaca, NY 14853 USA. [Morrice, D. R.] Univ Edinburgh, Edinburgh Genom, Ashworth Labs, Edinburgh EH9 3JT, Midlothian, Scotland. [Bichard, M.] English Guernsey Cattle Soc, Launceston PL15 9DP, Cornwall, England. [Luff, W. G. de L.] World Guernsey Cattle Federat, Castel GY5 7JD, Guernsey, England. RP Wiggans, GR (reprint author), ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. EM george.wiggans@ars.usda.gov FU European Union [289592-Gene2Farm]; USDA Agricultural Research Service [1245-31000-101-00] FX The research leading to these results has received funding from the European Union's Seventh Framework Programme for research, technological development and demonstration under grant agreement no. 289592-Gene2Farm. The authors thank Select Sires (Plain City, OH) and the American Guernsey Association (Columbus, OH) for project development and providing genotypes; the Council on Dairy Cattle Breeding (Bowie, MD) for supplying pedigree, performance, and genotypic data; and S. M. Hubbard (Animal Genomics and Improvement Laboratory, Agricultural Research Service, USDA, Beltsville, MD) for technical manuscript review. T. A. Cooper and G. R. Wiggans were supported by USDA Agricultural Research Service appropriated project 1245-31000-101-00, "Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information." The figure was created using Daniel's XL Toolbox add-in for Excel (version 6.60) by Daniel Kraus (Wurzburg, Germany). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by USDA. NR 24 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD JUL PY 2016 VL 99 IS 7 BP 5508 EP 5515 DI 10.3168/jds.2015-10445 PG 8 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DO7XA UT WOS:000377995100047 PM 27179857 ER PT J AU Bickhart, DM Hutchison, JL Null, DJ VanRaden, PM Cole, JB AF Bickhart, D. M. Hutchison, J. L. Null, D. J. VanRaden, P. M. Cole, J. B. TI Reducing animal sequencing redundancy by preferentially selecting animals with low-frequency haplotypes SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE whole-genome sequencing; redundancy; haplotype; inverse weight selection ID CATTLE; PREDICTIONS; IMPUTATION; ACCURACY; GENOTYPE; COVERAGE; GENOMES AB Many studies leverage targeted whole-genome sequencing (WGS) experiments to identify rare and causal variants within populations. As a natural consequence of their experimental design, many of these surveys tend to sequence redundant haplotype segments due to their high frequency in the base population, and the variants discovered within sequencing data are difficult to phase. We propose a new algorithm, called inverse weight selection (IWS), that preferentially selects individuals based on the cumulative presence of rare frequency haplotypes to maximize the efficiency of WGS surveys. To test the efficacy of this method, we used genotype data from 112,113 registered Holstein bulls derived from the US national dairy database. We demonstrate that IWS is at least 6.8% more efficient than previously published methods in selecting the least number of individuals required to sequence all haplotype segments >= 4% frequency in the US Holstein population. We also suggest that future surveys focus on sequencing homozygous haplotype segments as a first pass to achieve a 50% reduction in cost with an added benefit of phasing variant calls efficiently. Together, this new selection algorithm and experimental design suggestion significantly reduce the overall cost of variant discovery through WGS experiments, making surveys for causal variants influencing disease and production even more efficient. C1 [Bickhart, D. M.; Hutchison, J. L.; Null, D. J.; VanRaden, P. M.; Cole, J. B.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. RP Bickhart, DM (reprint author), ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. EM derek.bickhart@ars.usda.gov RI Cole, John/J-8571-2014; OI Cole, John/0000-0003-1242-4401; Bickhart, Derek/0000-0003-2223-9285 FU Agricultural Research Service of the United States Department of Agriculture (Washington, DC) [1265-31000-096-00]; Agricultural Food Research Initiative (AFRI) from the USDA National Institute of Food and Agriculture (NIFA, Washington, DC) Animal Health Program [2015-67015-22970] FX The Council on Dairy Cattle Breeding (Reynoldsburg, OH) and the Cooperative Dairy DNA Repository (Columbia, MO) are acknowledged for providing data used in this study. The authors were supported by appropriated project 1265-31000-096-00, "Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information," of the Agricultural Research Service of the United States Department of Agriculture (Washington, DC). D. M. B. was also supported by the Agricultural Food Research Initiative (AFRI) competitive grant number: 2015-67015-22970 from the USDA National Institute of Food and Agriculture (NIFA, Washington, DC) Animal Health Program. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 19 TC 0 Z9 0 U1 2 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD JUL PY 2016 VL 99 IS 7 BP 5526 EP 5534 DI 10.3168/jds.2015-10347 PG 9 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DO7XA UT WOS:000377995100049 PM 27085415 ER PT J AU Benjamin, AL Korkmaz, FT Elsasser, TH Kerr, DE AF Benjamin, A. L. Korkmaz, F. T. Elsasser, T. H. Kerr, D. E. TI Neonatal lipopolysaccharide exposure does not diminish the innate immune response to a subsequent lipopolysaccharide challenge in Holstein bull calves SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE early life lipopolysaccharide; inter-animal variation; inflammatory response ID ESCHERICHIA-COLI MASTITIS; MAMMARY EPITHELIAL-CELLS; DERMAL FIBROBLAST RESPONSE; BETWEEN-COW VARIATION; DAIRY-COWS; STAPHYLOCOCCUS-AUREUS; ENDOTOXIN TOLERANCE; INTRAMAMMARY INFECTIONS; PRENATAL EXPOSURE; DNA METHYLATION AB The innate immune response following experimental mastitis is quite variable between individual dairy cattle. An inflammatory response that minimizes collateral damage to the mammary gland while still effectively resolving the infection following pathogen exposure is beneficial to dairy producers. The ability of a lipopolysaccharide (LPS) exposure in early life to generate a low-responding phenotype and thus reduce the inflammatory response to a later-life LPS challenge was investigated in neonatal hull calves. Ten Holstein bull calves were randomly assigned to either an early life LPS (ELL) group (n = 5) or an early life saline (ELS) group (n = 5). At 7 d of age, calves received either LPS or saline, and at 32 d of age, all calves were challenged with an intravenous dose of LPS to determine the effect of the early life treatment (LPS or saline) on the immune response generated toward a subsequent LPS challenge. Dermal fibroblast and monocyte-derived macrophage cultures from each calf were established at age 20 and 27 d, respectively, to model sustained effects from the early life LPS exposure on gene expression and protein production of components within the ITS response pathway. The ELL calves had greater levels of plasma IL-6 and tumor necrosis factor-a than the ELS calves following the early life LPS or saline treatments. However, levels of these 2 immune markers were similar between ELL and ELS calves when both groups were subsequently challenged with LPS. A comparison of the in vitro LPS responses of the ELL and ELS calves revealed similar patterns of protein production and gene expression following an LPS challenge of both dermal fibroblast and monocyte-derived macrophage cultures established from the treatment groups. Whereas an early life exposure to LPS did not result in a dampened inflammatory response toward a later LPS challenge in these neonatal hull calves, the potential that exposure to inflammation or stress in early life or in utero can create an offspring with a low-responding phenotype as an adult is intriguing and has been documented in rodents. Further work is needed to determine if an inflammatory exposure in utero in a dairy animal would result in a low-responding innate immune phenotype. C1 [Benjamin, A. L.; Korkmaz, F. T.; Kerr, D. E.] Univ Vermont, Coll Agr & Life Sci, Dept Anim & Vet Sci, Burlington, VT 05405 USA. [Elsasser, T. H.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. RP Kerr, DE (reprint author), Univ Vermont, Coll Agr & Life Sci, Dept Anim & Vet Sci, Burlington, VT 05405 USA. EM david.kerr@uvm.edu FU USDA-HATCH grant from the Vermont Agricultural Experiment Station [VT-HO1417MS] FX The authors thank Green Mountain Dairy in Sheldon, Vermont, for providing the calves for this study. Additionally, Matt Bodette and Scott Shumway at the Paul Miller Research Center at the University of Vermont transported the calves to the research farm and aided in the set-up and care of the calves during the trial. Last, many thanks are due to the undergraduate students that helped in feeding and caring for the calves, and collecting samples during the experiment. Funding for this research was supported by a USDA-HATCH grant from the Vermont Agricultural Experiment Station (VT-HO1417MS). NR 60 TC 0 Z9 0 U1 6 U2 7 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD JUL PY 2016 VL 99 IS 7 BP 5750 EP 5763 DI 10.3168/jds.2015-10804 PG 14 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DO7XA UT WOS:000377995100072 PM 27108165 ER PT J AU Bougouin, A Leytem, A Dijkstra, J Dungan, RS Kebreab, E AF Bougouin, Adeline Leytem, April Dijkstra, Jan Dungan, Robert S. Kebreab, Ermias TI Nutritional and Environmental Effects on Ammonia Emissions from Dairy Cattle Housing: A Meta-Analysis SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Review ID MILK UREA NITROGEN; GREENHOUSE-GAS EMISSIONS; DIETARY CRUDE PROTEIN; OPEN-LOT DAIRIES; LIVESTOCK BUILDINGS; METHANE EMISSIONS; DEGRADED PROTEIN; MANURE EXCRETION; FREESTALL DAIRY; ENTERIC METHANE AB Nitrogen excreted in dairy manure can be potentially transformed and emitted as NH3, which can create livestock and human respiratory problems and be an indirect source of N2O. The objectives of this study were to: (i) investigate environmental factors influencing NH3 emissions from dairy housing; and (ii) identify key explanatory variables in the NH3 emissions prediction from dairy housing using a meta-analytical approach. Data from 25 studies were used for the preliminary analysis, and data from 10 studies reporting 87 treatment means were used for the meta-analysis. Season and flooring type significantly affected NH3 emissions. For nutritional effect analysis, the between-study variability (heterogeneity) of mean NH3 emission was estimated using random-effect models and had a significant effect (P < 0.01). Therefore, random-effect models were extended to mixed-effect models to explain heterogeneity regarding the available dietary and animal variables. The final mixed-effect model included milk yield, dietary crude protein, and dry matter intake separately, explaining 45.5% of NH3 emissions heterogeneity. A unit increase in milk yield (kg d(-1)) resulted in a 4.9 g cow(-1) d(-1) reduction in NH3 emissions, and a unit increase in dietary crude protein content (%) and dry matter intake (kg d(-1)) resulted in 10.2 and 16.3 g cow(-1) d(-1) increases in NH3 emissions, respectively, in the scope of this study. These results can be further used to help identify mitigation strategies to reduce NH3 emissions from dairy housing by developing predictive models that could determine variables with strong association with NH3 emissions. C1 [Bougouin, Adeline; Dijkstra, Jan] Wageningen Univ, Anim Nutr Grp, Wageningen, Netherlands. [Bougouin, Adeline; Kebreab, Ermias] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA. [Leytem, April; Dungan, Robert S.] USDA ARS, Kimberly, ID 83341 USA. RP Bougouin, A (reprint author), Wageningen Univ, Anim Nutr Grp, Wageningen, Netherlands.; Bougouin, A (reprint author), Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA. EM ad.bougouin@gmail.com RI Dijkstra, Jan/G-7680-2011; Kebreab, Ermias/E-9113-2012 OI Dijkstra, Jan/0000-0003-3728-6885; Kebreab, Ermias/0000-0002-0833-1352 NR 72 TC 1 Z9 1 U1 18 U2 35 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2016 VL 45 IS 4 BP 1123 EP 1132 DI 10.2134/jeq2015.07.0389 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DP9ZJ UT WOS:000378856400001 PM 27380059 ER PT J AU Trabue, SL Kerr, BJ Bearson, BL Hur, M Parkin, T Wurtele, ES Ziemer, CJ AF Trabue, Steven L. Kerr, Brian J. Bearson, Bradley L. Hur, Manhoi Parkin, Timothy Wurtele, Eve S. Ziemer, Cherrie J. TI Microbial Community and Chemical Characteristics of Swine Manure during Maturation SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID GREENHOUSE-GAS EMISSIONS; GROWING-FINISHING PIGS; DIETARY CRUDE PROTEIN; DRIED DISTILLERS GRAINS; STORED PIGGERY WASTES; VOLATILE FATTY-ACIDS; NITROGEN-EXCRETION; ODOROUS COMPOUNDS; AMMONIA EMISSION; HYDROGEN-SULFIDE AB Swine diet formulations have the potential to lower animal emissions, including odor and ammonia (NH3). The purpose of this study was to determine the impact of manure storage duration on manure chemical and microbial properties in swine feeding trials. Three groups of 12 pigs were fed a standard corn-soybean meal diet over a 13-wk period. Urine and feces were collected at each feeding and transferred to 12 manure storage tanks. Manure chemical characteristics and headspace gas concentrations were monitored for NH3, hydrogen sulfide (H2S), volatile fatty acids, phenols, and indoles. Microbial analysis of the stored manure included plate counts, community structure (denaturing gradient gel electrophoresis), and metabolic function (Biolog). All odorants in manure and headspace gas concentrations were significantly (p < 0.01) correlated for length of storage using quadratic equations, peaking after Week 5 for all headspace gases and most manure chemical characteristics. Microbial community structure and metabolic utilization patterns showed continued change throughout the 13-wk trial. Denaturing gradient gel electrophoresis species diversity patterns declined significantly (p < 0.01) with time as substrate utilization declined for sugars and certain amino acids, but functionality increased in the utilization of short chain fatty acids as levels of these compounds increased in manure. Studies to assess the effect of swine diet formulations on manure emissions for odor need to be conducted for a minimum of 5 wk. Efforts to determine the impact of diets on greenhouse gas emissions will require longer periods of study (>13 wk). C1 [Trabue, Steven L.; Kerr, Brian J.; Bearson, Bradley L.; Parkin, Timothy; Ziemer, Cherrie J.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Hur, Manhoi; Wurtele, Eve S.] Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA 50011 USA. RP Trabue, SL (reprint author), USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. EM steven.trabue@ars.usda.gov OI Wurtele, Eve/0000-0003-1552-9495 NR 80 TC 2 Z9 2 U1 6 U2 10 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2016 VL 45 IS 4 BP 1144 EP 1152 DI 10.2134/jeq2015.09.0446 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DP9ZJ UT WOS:000378856400003 PM 27380061 ER PT J AU Miller, DN Spiehs, MJ Varel, VH Woodbury, BL Wells, JE Berry, ED AF Miller, Daniel N. Spiehs, Mindy J. Varel, Vincent H. Woodbury, Bryan L. Wells, James E. Berry, Elaine D. TI Distillers By-Product Cattle Diets Enhance Reduced Sulfur Gas Fluxes from Feedlot Soils and Manures SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID BEEF-CATTLE; ODORANT PRODUCTION; AMMONIA EMISSIONS; ESCHERICHIA-COLI; GRAINS; SOLUBLES; SLURRIES; CHAMBER AB Total reduced sulfur (TRS) emissions from animal feeding operations are a concern with increased feeding of high-sulfur distillers by-products. Three feeding trials were conducted to evaluate feeding wet distillers grain plus solubles (WDGS) on TRS fluxes. Fresh manure was collected three times during Feeding Trial 1 from cattle fed 0, 20, 40, and 60% WDGS. Fluxes of TRS from 40 and 60% WDGS manures were 3- to 13-fold greater than the 0 and 20% WDGS manures during the first two periods. In the final period, TRS flux from 60% WDGS was 5- to 22-fold greater than other WDGS manures. During Feeding Trial 2, 0 and 40% WDGS diets on four dates were compared in feedlot-scale pens. On two dates, fluxes from mixed manure and soil near the feed bunk were 3.5-fold greater from 40% WDGS pens. After removing animals, soil TRS flux decreased 82% over 19 d but remained 50% greater in 40% WDGS pens, principally from the wetter pen edges (1.9-fold greater than the drier central mound). During two cycles of cattle production in Feeding Trial 3, TRS soil fluxes were 0.3- to 4-fold greater over six dates for pens feeding WDGS compared with dry-rolled corn diet and principally from wetter pen edges. Soil TRS flux correlated with % WDGS, total N, total P, manure pack temperature, and surface temperature. Consistent results among these three trials indicate that TRS fluxes increase by two-to fivefold when cattle were fed greater levels of WDGS, but specific manure management practices may help control TRS fluxes. C1 [Miller, Daniel N.] USDA ARS, Agroecosyst Management Res Unit, 354 Filley Hall,UNL East Campus, Lincoln, NE 68583 USA. [Spiehs, Mindy J.; Varel, Vincent H.; Woodbury, Bryan L.; Wells, James E.; Berry, Elaine D.] USDA ARS, US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. RP Miller, DN (reprint author), USDA ARS, Agroecosyst Management Res Unit, 354 Filley Hall,UNL East Campus, Lincoln, NE 68583 USA. EM dan.miller@ars.usda.gov OI Miller, Daniel/0000-0003-3476-487X NR 26 TC 0 Z9 0 U1 1 U2 2 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2016 VL 45 IS 4 BP 1161 EP 1168 DI 10.2134/jeq2015.09.0480 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA DP9ZJ UT WOS:000378856400005 PM 27380063 ER PT J AU Owens, J Clough, TJ Laubach, J Hunt, JE Venterea, RT Phillips, RL AF Owens, Jen Clough, Tim J. Laubach, Johannes Hunt, John E. Venterea, Rodney T. Phillips, Rebecca L. TI Nitrous Oxide Fluxes, Soil Oxygen, and Denitrification Potential of Urine- and Non-Urine-Treated Soil under Different Irrigation Frequencies SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID PASTURE SOIL; NITRIFIER DENITRIFICATION; N2O EMISSIONS; CATTLE URINE; NEW-ZEALAND; FERTILIZATION; COMPACTION; GRASSLAND; CHAMBER; NITRATE AB Despite increased use of irrigation to improve forage quality and quantity for grazing cattle (Bos taurus, Linnaeus), there is a lack of data that assess how irrigation practices influence nitrous oxide (N2O) emissions from urine-affected soils. Irrigation effects on soil oxygen (O-2) availability, a primary controller of N2O fluxes, is poorly understood. It was hypothesized that increased irrigation frequency would result in lower N2O emissions by increasing soil moisture and decreasing soil O-2 concentrations. This would favor more N2O reduction to dinitrogen (N-2). We examined effects of high (3-d) versus low (6-d) irrigation frequency with and without bovine urine addition to pasture. Nitrous oxide fluxes were measured daily for 35 d. Soil O-2, temperature, and water content were continuously measured at multiple depths. Inorganic nitrogen, organic carbon, and soil pH were measured at 6-d intervals. Measurements of denitrification enzyme activity with and without acetylene inhibition were used to infer the N2O/(N2O + N-2) ratio. The N2O/(N2O + N-2) ratio was lower under high-compared with low-frequency irrigation, suggesting greater potential for N2O reduction to N-2 with more frequent irrigation. Although N2O fluxes were increased by urine addition, they were not affected by irrigation frequency. Soil O-2 decreased temporarily after urine deposition, but O-2 dynamics did not explain N2O dynamics. Relative soil gas diffusivity (DP/DO) was a better predictor of N2O fluxes than O-2 concentration. On a freedraining soil, increasing irrigation frequency while providing the same total water volume did not enhance N2O emissions under ruminant urine patches in a grazed pasture. C1 [Owens, Jen; Clough, Tim J.] Lincoln Univ, Dept Soil & Phys Sci, POB 85084, Lincoln 7647, New Zealand. [Laubach, Johannes; Hunt, John E.; Phillips, Rebecca L.] Landcare Res, POB 69040, Lincoln 7640, New Zealand. [Venterea, Rodney T.] Univ Minnesota, USDA ARS, Soil & Water Management Res Unit, St Paul, MN 55108 USA. [Venterea, Rodney T.] Univ Minnesota, Dept Soil & Water & Climate, St Paul, MN 55108 USA. RP Owens, J (reprint author), Lincoln Univ, Dept Soil & Phys Sci, POB 85084, Lincoln 7647, New Zealand. EM jen.owens@lincolnuni.ac.nz OI Phillips, Rebecca/0000-0003-3881-9065 FU CRI Core Funding from New Zealand's Ministry of Business, Innovation and Employment FX This research was undertaken with CRI Core Funding to Landcare Research from New Zealand's Ministry of Business, Innovation and Employment. The authors thank Neil Smith and Greg Curlin for help in the field, the Centre for Environment and Soil Research at Lincoln University for the loaned equipment, and Synlait Farms (now renamed Purata) for allowing access to one of their dairy farms. NR 64 TC 1 Z9 1 U1 19 U2 29 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2016 VL 45 IS 4 BP 1169 EP 1177 DI 10.2134/jeq2015.10.0516 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DP9ZJ UT WOS:000378856400006 PM 27380064 ER PT J AU Venterea, RT Coulter, JA Dolan, MS AF Venterea, Rodney T. Coulter, Jeffrey A. Dolan, Michael S. TI Evaluation of Intensive "4R" Strategies for Decreasing Nitrous Oxide Emissions and Nitrogen Surplus in Rainfed Corn SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID N2O EMISSIONS; FERTILIZER APPLICATION; ANHYDROUS AMMONIA; SPLIT APPLICATION; CROPPING SYSTEMS; CARBON-DIOXIDE; TEXTURED SOIL; MAIZE YIELD; NO-TILL; MANAGEMENT AB The "4R" approach of using the right rate, right source, right timing, and right placement is an accepted framework for increasing crop N use efficiency. However, modifying only one 4R component does not consistently reduce nitrous oxide (N2O) emissions. Our objective was to determine if N fertilizer applied in three split applications (Sp), by itself or combined with changes in N source and rate, could improve N recovery efficiency (NRE) and N surplus (NS) and decrease N2O emissions. Over two corn (Zea mays L.) growing seasons in Minnesota, N2O emissions ranged from 0.6 to 0.9 kg N ha(-1). None of the treatment combinations affected grain yield. Compared with urea applied in a single application at the recommended N rate, Sp by itself did not improve NRE or NS and did not decrease N2O. Combining Sp with urease and nitrification inhibitors and/or a 15% reduction in N rate increased NRE from 57 to >73% and decreased NS by >20 kg N ha(-1). The only treatment that decreased N2O (by 20-53%) was Sp combined with inhibitors and reduced N rate. Emissions of N2O were more strongly correlated with NS calculated from grain N uptake (R-2 = 0.61) compared with whole-plant N uptake (r(2) = 0.39), possibly because most N losses occurred before grain filling. Optimizing both application timing and N source can allow for a moderate reduction in N rate that does not affect grain yield but decreases N2O. Grain-based NS may be a more useful indicator of N2O emissions than whole-plant-based NS. C1 [Venterea, Rodney T.; Dolan, Michael S.] USDA ARS, Soil & Water Management Unit, 1991 Upper Buford Circle, St Paul, MN 55108 USA. [Venterea, Rodney T.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Coulter, Jeffrey A.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. RP Venterea, RT (reprint author), USDA ARS, Soil & Water Management Unit, 1991 Upper Buford Circle, St Paul, MN 55108 USA.; Venterea, RT (reprint author), Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. EM Venterea@umn.edu FU Minnesota Corn Research and Promotion Council FX The authors thank Ms. Libby Mills and Mr. Paxer Vang for assistance with field and lab work and Dr. Greg Schwab and Koch Agronomic Services for providing the SuperU product. This work was supported in part by a grant from the Minnesota Corn Research and Promotion Council. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA or the University of Minnesota. NR 49 TC 1 Z9 1 U1 16 U2 33 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2016 VL 45 IS 4 BP 1186 EP 1195 DI 10.2134/jeq2016.01.0024 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DP9ZJ UT WOS:000378856400008 PM 27380066 ER PT J AU Collick, AS Veith, TL Fuka, DR Kleinman, PJA Buda, AR Weld, JL Bryant, RB Vadas, PA White, MJ Harmel, RD Easton, ZM AF Collick, Amy S. Veith, Tamie L. Fuka, Daniel R. Kleinman, Peter J. A. Buda, Anthony R. Weld, Jennifer L. Bryant, Ray B. Vadas, Peter A. White, Mike J. Harmel, R. Daren Easton, Zachary M. TI Improved Simulation of Edaphic and Manure Phosphorus Loss in SWAT SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID SURFACE-APPLIED MANURES; WATER ASSESSMENT-TOOL; NO-TILL SOILS; POULTRY LITTER; DISSOLVED PHOSPHORUS; RAINFALL INTENSITY; SOURCE AREAS; RUNOFF LOSS; MODEL; MANAGEMENT AB Watershed models such as the Soil Water Assessment Tool (SWAT) and the Agricultural Policy Environmental EXtender (APEX) are widely used to assess the fate and transport of agricultural nutrient management practices on soluble and particulate phosphorus (P) loss in runoff. Soil P-cycling routines used in SWAT2012 revision 586, however, do not simulate the short-term effects of applying a concentrated source of soluble P, such as manure, to the soil surface where it is most vulnerable to runoff. We added a new set of soil P routines to SWAT2012 revision 586 to simulate surface-applied manure at field and subwatershed scales within Mahantango Creek watershed in south-central Pennsylvania. We corroborated the new P routines and standard P routines in two versions of SWAT (conventional SWAT, and a topographically driven variation called TopoSWAT) for a total of four modeling "treatments". All modeling treatments included 5 yr of measured data under field-specific, historical management information. Short-term "wash off" processes resulting from precipitation immediately following surface application of manures were captured with the new P routine whereas the standard routines resulted in losses regardless of manure application. The new routines improved sensitivity to key factors in nutrient management (i.e., timing, rate, method, and form of P application). Only the new P routines indicated decreases in soluble P losses for dairy manure applications at 1, 5, and 10 d before a storm event. The new P routines also resulted in more variable P losses when applying manure versus commercial fertilizer and represented increases in total P losses, as compared with standard P routines, with rate increases in dairy manure application (56,000 to 84,000 L ha(-1)). The new P routines exhibited greater than 50% variation among proportions of organic, particulate, and soluble P corresponding to spreading method. In contrast, proportions of P forms under the standard P routines varied less than 20%. Results suggest similar revisions to other agroecosystem watershed models would be appropriate. C1 [Collick, Amy S.; Veith, Tamie L.; Kleinman, Peter J. A.; Buda, Anthony R.; Bryant, Ray B.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Fuka, Daniel R.; Easton, Zachary M.] Virginia Tech, Dept Biol Syst Engn, Blacksburg, VA 24061 USA. [Weld, Jennifer L.] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [Vadas, Peter A.] USDA ARS, Dairy Forage Res Ctr, Madison, WI 53706 USA. [White, Mike J.; Harmel, R. Daren] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. RP Collick, AS (reprint author), USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. EM amy.collick@ars.usda.gov RI Veith, Tamie/H-4911-2011 FU USDA-NRCS Conservation Innovation Grants (CIG) FX The authors thank the USDA-NRCS Conservation Innovation Grants (CIG) for financial support in part as well as the strong collaborations of the CIG project teams. The authors also thank Gordan Folmar and Jim Richards from USDA-ARS for providing watershed hydrology data and field management information, respectively. NR 54 TC 0 Z9 0 U1 12 U2 16 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2016 VL 45 IS 4 BP 1215 EP 1225 DI 10.2134/jeq2015.03.0135 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA DP9ZJ UT WOS:000378856400011 PM 27380069 ER PT J AU Scudiero, E Lesch, SM Corwin, DL AF Scudiero, Elia Lesch, Scott M. Corwin, Dennis L. TI Validation of Sensor-Directed Spatial Simulated Annealing Soil Sampling Strategy SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID ELECTRICAL-CONDUCTIVITY; CONSTRAINED OPTIMIZATION; ELECTROMAGNETIC INDUCTION; MODEL; SCALE; AGRICULTURE; SALINITY; INFORMATION; VARIABILITY; PREDICTION AB Soil spatial variability has a profound influence on most agronomic and environmental processes at field and landscape scales, including site-specific management, vadose zone hydrology and transport, and soil quality. Mobile sensors are a practical means of mapping spatial variability because their measurements serve as a proxy for many soil properties, provided a sensor-soil calibration is conducted. A viable means of calibrating sensor measurements over soil properties is through linear regression modeling of sensor and target property data. In the present study, two sensor-directed, model-based, sampling scheme delineation methods were compared to validate recent applications of soil apparent electrical conductivity (ECa)-directed spatial simulated annealing against the more established ECa-directed response surface sampling design (RSSD) approach. A 6.8-ha study area near San Jacinto, CA, was surveyed for ECa, and 30 soil sampling locations per sampling strategy were selected. Spatial simulated annealing and RSSD were compared for sensor calibration to a target soil property (i.e., salinity) and for evenness of spatial coverage of the study area, which is beneficial for mapping nontarget soil properties (i.e., those not correlated with ECa). The results indicate that the linear modeling ECa-salinity calibrations obtained from the two sampling schemes provided salinity maps characterized by similar errors. The maps of nontarget soil properties show similar errors across sampling strategies. The Spatial Simulated Annealing methodology is, therefore, validated, and its use in agronomic and environmental soil science applications is justified. C1 [Scudiero, Elia; Corwin, Dennis L.] USDA ARS, US Salin Lab, 450 West Big Springs Rd, Riverside, CA 92507 USA. [Lesch, Scott M.] Riverside Publ Util, 3435 14th St, Riverside, CA 92501 USA. RP Scudiero, E (reprint author), USDA ARS, US Salin Lab, 450 West Big Springs Rd, Riverside, CA 92507 USA. EM scudiero@dmsa.unipd.it OI Scudiero, Elia/0000-0003-4944-721X NR 46 TC 0 Z9 0 U1 5 U2 7 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2016 VL 45 IS 4 BP 1226 EP 1233 DI 10.2134/jeq2015.09.0458 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA DP9ZJ UT WOS:000378856400012 PM 27380070 ER PT J AU Hengen, TJ Sieverding, HL Cole, NA Ham, JM Stone, JJ AF Hengen, Tyler J. Sieverding, Heidi L. Cole, Noel A. Ham, Jay M. Stone, James J. TI Eco-Efficiency Model for Evaluating Feedlot Rations in the Great Plains, United States SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID GREENHOUSE-GAS EMISSIONS; LIFE-CYCLE ASSESSMENT; BEEF-CATTLE PRODUCTION; PRODUCTION SYSTEM; WESTERN CANADA; ENVIRONMENTAL IMPACTS; DAIRY; MITIGATION; METHANE; SUSTAINABILITY AB Environmental impacts attributable to beef feedlot production provide an opportunity for economically linked efficiency optimization. Eco-efficiency models are used to optimize production and processes by connecting and quantifying environmental and economic impacts. An adaptable, objective eco-efficiency model was developed to assess the impacts of dietary rations on beef feedlot environmental and fiscal cost. The hybridized model used California Net Energy System modeling, life cycle assessment, principal component analyses (PCA), and economic analyses. The model approach was based on 38 potential feedlot rations and four transportation scenarios for the US Great Plains for each ration to determine the appropriate weight of each impact. All 152 scenarios were then assessed through a nested PCA to determine the relative contributing weight of each impact and environmental category to the overall system. The PCA output was evaluated using an eco-efficiency model. Results suggest that water, ecosystem, and human health emissions were the primary impact category drivers for feedlot eco-efficiency scoring. Enteric CH4 emissions were the greatest individual contributor to environmental performance (5.7% of the overall assessment), whereas terrestrial ecotoxicity had the lowest overall contribution (0.2% of the overall assessment). A well-balanced ration with mid-range dietary and processing energy requirements yielded the most eco- and environmentally efficient system. Using these results, it is possible to design a beef feed ration that is more economical and environmentally friendly. This methodology can be used to evaluate eco-efficiency and to reduce researcher bias of other complex systems. C1 [Hengen, Tyler J.; Sieverding, Heidi L.; Stone, James J.] South Dakota Sch Mines & Technol, Dept Civil & Environm Engn, Rapid City, SD 57701 USA. [Cole, Noel A.] USDA ARS, Conservat & Prod Res Lab, 2300 Expt Stn Rd, Bushland, TX 79012 USA. [Ham, Jay M.] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80525 USA. RP Stone, JJ (reprint author), South Dakota Sch Mines & Technol, Dept Civil & Environm Engn, Rapid City, SD 57701 USA. EM james.stone@sdsmt.edu FU Colorado State University FX Funding for the project was partially provided by a sustainability collaboration award from Colorado State University. NR 54 TC 0 Z9 0 U1 15 U2 16 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2016 VL 45 IS 4 BP 1234 EP 1242 DI 10.2134/jeq2015.09.0464 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DP9ZJ UT WOS:000378856400013 PM 27380071 ER PT J AU King, SE Osmond, DL Smith, J Burchell, MR Dukes, M Evans, RO Knies, S Kunickis, S AF King, S. E. Osmond, D. L. Smith, J. Burchell, M. R. Dukes, M. Evans, R. O. Knies, S. Kunickis, S. TI Effects of Riparian Buffer Vegetation and Width: A 12-Year Longitudinal Study SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID SHALLOW GROUNDWATER QUALITY; COASTAL-PLAIN; WATER-QUALITY; NITRATE DYNAMICS; NITROGEN REMOVAL; ORGANIC-CARBON; DENITRIFICATION; FOREST; DRAINAGE; AVAILABILITY AB Agricultural contributions of nitrogen are a serious concern for many water resources and have spurred the implementation of riparian buffer zones to reduce groundwater nitrate (NO3). The optimum design for buffers is subject to debate, and there are few long-term studies. The objective of this project was to determine the effectiveness over time (12 yr) of buffer types (trees, switchgrass, fescue, native, and a control) and buffer widths (8 and 15 m) by measuring groundwater NO3-N and dissolved organic carbon (DOC) trends. At the intermediate groundwater depth (1.5-2.1 m), NO3-N reduction effectiveness was 2.5 times greater (46 vs. 16%) for the wider buffer, and, regardless of width, buffer effectiveness increased 0.62% yr(-1). Buffer vegetative type was never statistically significant. In the deep-groundwater depth (2.1-3.5 m), there was no change in NO3-N removal over time, although the statistical interaction of width and vegetative type indicated a wide range of removal rates (19-82%). The DOC concentrations were analyzed at the field/buffer and buffer/stream sampling locations. Depending on location position and groundwater sampling depth, DOC concentrations ranged from 1.6 to 2.8 mg L-1 at Year 0 and increased at a rate of 0.13 to 0.18 mg L-1 yr(-1) but always remained low (<= 5.0 mg L-1). Greater DOC concentrations in the intermediate-depth groundwater did not increase NO3-N removal; redox measurements indicated intermittent reduced soil conditions may have been limiting. This study suggests that riparian buffer width, not vegetation, is more important for NO3-N removal in the middle coastal plain of North Carolina for a newly established buffer. C1 [King, S. E.] Michael Baker Engn, 8000 Regency Pkwy,Suite 600, Cary, NC 27518 USA. [Osmond, D. L.; Knies, S.] NC State Univ, Dept Soil Sci, Box 7619, Raleigh, NC 27695 USA. [Smith, J.] NC State Univ, Dept Stat, Box 8203, Raleigh, NC 27695 USA. [Burchell, M. R.; Evans, R. O.] NC State Univ, Dept Biol & Agr Engn, Box 7625, Raleigh, NC 27695 USA. [Dukes, M.] Univ Florida, Dept Biol & Agr Engn, Box 110570, Gainesville, FL 32611 USA. [Kunickis, S.] USDA, Off Pest Management, 1400 Independence Ave,Rm 3871, Washington, DC 20250 USA. RP Osmond, DL (reprint author), NC State Univ, Dept Soil Sci, Box 7619, Raleigh, NC 27695 USA. EM deanna_osmond@ncsu.edu FU NC Department of Environment and Natural Resources (NCDENR) Conservation Reserve Enhancement Program; USEPA pass-through from NCDENR [319]; Graduate Student Assistance program of the College of Agriculture and Life Sciences at North Carolina State University FX This work was supported by NC Department of Environment and Natural Resources (NCDENR) Conservation Reserve Enhancement Program, USEPA 319 pass-through from NCDENR, and the Graduate Student Assistance program of the College of Agriculture and Life Sciences at North Carolina State University. Wesley Childres of the Soils Department at NC State University provided technical support for groundwater monitoring. NR 51 TC 1 Z9 1 U1 20 U2 28 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2016 VL 45 IS 4 BP 1243 EP 1251 DI 10.2134/jeq2015.06.0321 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DP9ZJ UT WOS:000378856400014 PM 27380072 ER PT J AU Singh, NK Reyes, WM Bernhardt, ES Bhattacharya, R Meyer, JL Knoepp, JD Emanuel, RE AF Singh, Nitin K. Reyes, Wilmer M. Bernhardt, Emily S. Bhattacharya, Ruchi Meyer, Judy L. Knoepp, Jennifer D. Emanuel, Ryan E. TI Hydro-Climatological Influences on Long-Term Dissolved Organic Carbon in a Mountain Stream of the Southeastern United States SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID CLIMATE-CHANGE; FORESTED WATERSHEDS; NEW-YORK; PERSULFATE OXIDATION; HIGH-TEMPERATURE; ACID DEPOSITION; BOREAL LAKES; DOC EXPORTS; PEAT SOILS; TRENDS AB In the past decade, significant increases in surface water dissolved organic carbon (DOC) have been reported for large aquatic ecosystems of the Northern Hemisphere and have been attributed variously to global warming, altered hydrologic conditions, and atmospheric deposition, among other factors. We analyzed a 25-yr DOC record (1988-2012) available for a forested headwater stream in the United States and documented two distinct regimes of stream DOC trends. From 1988 to 2001, annual mean volume-weighted DOC concentration (DOCvw, mg L-1) and annual DOC flux (kg ha(-1) yr(-1)) declined by 34 and 56%, respectively. During 1997 to 2012, the decline in DOCvw and DOC flux increased by 141 and 165%, respectively. Declining DOCvw from 1988 to 2001 corresponded to a decline in growing season runoff, which has the potential to influence mobilization of DOC from uplands to streams. Increasing DOCvw from 1997 to 2012 corresponded to increased precipitation early in the growing season and to an increase in the number and intensity of short-duration fall storms capable of mobilizing long-accrued DOC from forest litter and soils. In contrast, total annual runoff declined throughout the period. Rising air temperature, atmospheric acid deposition, and nitrogen depositions did not offer any plausible explanation for the observed bidirectional annual trends of stream DOCvw. Our study highlights the critical role of long-term datasets and analyses for understanding the impacts of climate change on carbon and water cycles and associated functions of aquatic and terrestrial ecosystems. C1 [Singh, Nitin K.; Reyes, Wilmer M.; Bhattacharya, Ruchi; Emanuel, Ryan E.] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Bernhardt, Emily S.] Duke Univ, Dept Biol, Durham, NC 27708 USA. [Bernhardt, Emily S.] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Meyer, Judy L.] Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. [Knoepp, Jennifer D.] US Forest Serv, Coweeta Hydrol Lab, USDA, Southern Res Stn, Otto, NC 28763 USA. RP Singh, NK (reprint author), N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. EM nksingh01@gmail.com RI Bernhardt, Emily/D-9940-2011; Emanuel, Ryan/C-3796-2012 OI Bernhardt, Emily/0000-0003-3031-621X; Emanuel, Ryan/0000-0002-2166-1698 FU Department of Forestry and Environmental Resources at North Carolina State University; US Department of Interior Southeast Climate Science Center; USDA Forest Service Southern Research Station; NSF [DEB-0218001, DEB-0823293] FX The study was funded in part by the Department of Forestry and Environmental Resources at North Carolina State University. The US Department of Interior Southeast Climate Science Center supported N.K. Singh through a Global Change Fellowship. Additional support was provided by the USDA Forest Service Southern Research Station and by NSF awards DEB-0218001 and DEB-0823293 to the Coweeta Long-Term Ecological Research program. NR 84 TC 1 Z9 1 U1 8 U2 13 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2016 VL 45 IS 4 BP 1286 EP 1295 DI 10.2134/jeq2015.10.0537 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DP9ZJ UT WOS:000378856400019 PM 27380077 ER PT J AU Zhang, XC Liu, BL Liu, B Zhang, GH AF Zhang, X. C. (John) Liu, B. L. Liu, B. Zhang, G. H. TI Quantifying Sediment Provenance Using Multiple Composite Fingerprints in a Small Watershed in Oklahoma SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID SUSPENDED SEDIMENT; RIVER-BASINS; GULLIED CATCHMENT; BANK EROSION; UK; UNCERTAINTY; STREAM; TRANSPORT; SOILS; RATES AB Quantitative information on sediment provenance is needed for improved calibration and validation of process-based soil erosion models. However, sediment source data are often limited due to difficulties in directly measuring source contributions at a watershed scale. Our objectives in this study were to estimate sediment source contributions in a 15-km(2) watershed using analytical solutions to a three end-member mixing model using multiple composite fingerprints and to compare the results with those estimated with a single radionuclide, Cs-137. Surface soil samples were collected from 23 croplands, 19 rangelands, and 26 gully banks in the watershed, and 31 geochemical elements were analyzed for each sample. The elements served as tracers and were screened using statistical tests and range checks. The mean concentrations of all the nonconflict tracer pairs were used in the mixing model to calculate source contributions for the three sources. Results showed that although source contributions were strongly influenced by topography and land use, gully or subsoil erosion was found to be the main source of fine sediment in most subwatersheds. This study demonstrates that estimated source contributions may vary substantially among different composite fingerprints and that the use of multiple composite fingerprints greatly improves accuracy while reducing uncertainty. The source contributions estimated using multiple composite fingerprints agreed well with those estimated with Cs-137, with a correlation coefficient of 0.69 for gully contributions. This good agreement increases our confidence in using the multiple composite fingerprint method to identify sediment provenance in relatively small watersheds. C1 [Zhang, X. C. (John)] Beijing Normal Univ, State Key Lab Earth Surface Proc & Resource Ecol, Beijing, Peoples R China. [Zhang, X. C. (John)] USDA ARS, Grazinglands Res Lab, 7207 W Cheyenne St, El Reno, OK 73036 USA. [Liu, B. L.] Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Gansu, Peoples R China. [Liu, B.] China Inst Water Resources & Hydropower Res, State Key Lab Simulat & Regulat Water Cycle River, Beijing, Peoples R China. RP Liu, BL (reprint author), Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Gansu, Peoples R China. EM liubenli@lzb.ac.cn OI Liu, Benli/0000-0002-7664-5142 FU State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University [2015-KF-09]; National Natural Science Foundation of China [41501008, 51309154]; Key Laboratory of Land Surface Process and Climate Change in Cold and Arid Regions, Chinese Academy of Sciences [LPCC201301] FX This work was partially supported by State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University (No. 2015-KF-09); by the National Natural Science Foundation of China (Grant No. 41501008, 51309154); and by the Key Laboratory of Land Surface Process and Climate Change in Cold and Arid Regions, Chinese Academy of Sciences (LPCC201301). NR 32 TC 1 Z9 1 U1 9 U2 14 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2016 VL 45 IS 4 BP 1296 EP 1302 DI 10.2134/jeq2015.10.0538 PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA DP9ZJ UT WOS:000378856400020 PM 27380078 ER PT J AU Barbarick, KA Ippolito, JA McDaniel, JP AF Barbarick, Kenneth A. Ippolito, James A. McDaniel, Jacob P. TI Path Analyses of Grain P, Zn, Cu, Fe, and Ni in a Biosolids-Amended Dryland Wheat Agroecosystem SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID PHOSPHORUS SORPTION; ORGANIC AMENDMENTS; SOIL AB Land application of biosolids is an effective means of recycling plant nutrients and is the primary method of biosolids reuse endorsed by the USEPA. One issue concerning biosolids application is the extent of the contribution of biosolids-borne plant nutrients to the overall crop concentration and uptake or removal of these nutrients. We studied the effects of biosolids application on wheat (Triticum aestivum L.) grain P, Zn, Cu, Fe, and Ni concentrations and uptake (removal) at two dryland agroecosystem sites from 1993 to 2014. We hypothesized that biosolids would have the greatest impact on wheat grain and uptake compared with ammonium bicarbonate-diethylenetriaminepentaacetic acid (AB-DTPA)-extractable nutrient levels, soil pH, or soil organic C concentrations. We used path analyses in combination with multiple linear regression to differentiate the direct, indirect, and total effects of cumulative biosolids applications, soil AB-DTPA, soil pH, and organic C. Biosolids rates, applied biennially from 1993 to 2014 at the beginning of a wheat-fallow rotation, were 0, 2.24, 4.48, 6.72, 8.96, and 11.2 Mg ha(-1). None of the parameters had significant direct, indirect, or total effects on grain concentrations. Biosolids applications had the greatest positive direct impact compared with AB-DTPA levels, soil pH, or soil organic C on P, Zn, Fe, and Ni uptake (removal), whereas AB-DTPA had the greatest positive direct impact on Cu uptake. Soil AB-DTPA, pH, and organic C directly affected some grain concentrations and cumulative uptake, but no consistent trends were noted. This pathway approach allowed differentiation between causation and simple correlation for the effects of cumulative biosolids applications on wheat P, Zn, Cu, Fe, and Ni cumulative uptake but did not provide these same results for grain concentrations. C1 [Barbarick, Kenneth A.; McDaniel, Jacob P.] Colorado State Univ, Dept Soil & Crop Sci, 200 W Lake St, Ft Collins, CO 80523 USA. [Ippolito, James A.] USDA ARS, NWISRL, 3793 North 3600 East, Kimberly, ID 83341 USA. RP Barbarick, KA (reprint author), Colorado State Univ, Dept Soil & Crop Sci, 200 W Lake St, Ft Collins, CO 80523 USA. EM ken.barbarick@colostate.edu FU Littleton/Englewood Wastewater Treatment Plant; Colorado State Agricultural Experiment Station FX This research was funded by the Littleton/Englewood Wastewater Treatment Plant and the Colorado State Agricultural Experiment Station. NR 29 TC 0 Z9 0 U1 7 U2 7 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2016 VL 45 IS 4 BP 1400 EP 1404 DI 10.2134/jeq2015.06.0303 PG 5 WC Environmental Sciences SC Environmental Sciences & Ecology GA DP9ZJ UT WOS:000378856400032 PM 27380090 ER PT J AU Huang, L Moore, PA Kleinman, PJA Elkin, KR Savin, MC Pote, DH Edwards, DR AF Huang, Lidong Moore, Philip A., Jr. Kleinman, Peter J. A. Elkin, Kyle R. Savin, Mary C. Pote, Daniel H. Edwards, Dwayne R. TI Reducing Phosphorus Runoff and Leaching from Poultry Litter with Alum: Twenty-Year Small Plot and Paired-Watershed Studies SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID CHEMICAL AMENDMENTS; SOIL-PHOSPHORUS; AMMONIA VOLATILIZATION; IRON AMENDMENTS; TREATED LITTER; DRINKING-WATER; AVAILABILITY; SOLUBILITY; SPECIATION; MEHLICH-3 AB Treating poultry litter with alum has been shown to lower ammonia (NH3) emissions and phosphorus (P) runoff losses. Two long-term studies were conducted to assess the effects of alum-treated poultry litter on P availability, leaching, and runoff under pasture conditions. From 1995 to 2015, litter was applied annually in a paired watershed study comparing alum-treated and untreated litter and in a small plot study comparing 13 treatments (an unfertilized control, four rates of alum-treated litter, four rates of untreated litter, and four rates of NH4NO3). In the paired watershed study, total P loads in runoff were 231% higher from pasture receiving untreated litter (1.96 kg P ha(-1)) than from that receiving alum-treated litter (0.85 kg P ha(-1)). In both studies, alum-treated litter resulted in significantly higher Mehlich III P (M3-P) and lower water-extractable P at the soil surface, reflecting greater retention of applied P and lesser availability of that P to runoff or leaching. In soils fertilized with alum-treated litter, M3-P was much higher when analyzed by inductively coupled argon plasma emission spectrometry than by colorimetry, possibly due to the formation of aluminum phytate. Indeed, alum-treated poultry litter leached less P over the 20-yr study: M3-P at 10 to 50 cm was 266% greater in plots fertilized with untreated litter (331 kg M3-P ha(-1)) than with alum-treated litter (124 kg M3-P ha(-1)). This research provides compelling evidence that treating poultry litter with alum provides short-term and long-term benefits to P conservation and water quality. C1 [Huang, Lidong] Nanjing Univ Informat Sci & Technol, Coll Appl Meteorol, Nanjing 210044, Jiangsu, Peoples R China. [Moore, Philip A., Jr.] Univ Arkansas, USDA ARS, Poultry Prod & Prod Safety Res Unit, Plant Sci 115, Fayetteville, AR 72701 USA. [Kleinman, Peter J. A.; Elkin, Kyle R.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Savin, Mary C.] Univ Arkansas, Crops Soils & Environm Sci Dept, Fayetteville, AR 72701 USA. [Pote, Daniel H.] USDA ARS, Small Farms Res Unit, Booneville, AR 72927 USA. [Edwards, Dwayne R.] Univ Kentucky, Biosyst & Agr Engn, Lexington, KY 40546 USA. RP Moore, PA (reprint author), Univ Arkansas, USDA ARS, Poultry Prod & Prod Safety Res Unit, Plant Sci 115, Fayetteville, AR 72701 USA. EM philipm@uark.edu NR 49 TC 0 Z9 0 U1 11 U2 13 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2016 VL 45 IS 4 BP 1413 EP 1420 DI 10.2134/jeq2015.09.0482 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA DP9ZJ UT WOS:000378856400034 PM 27380092 ER PT J AU Moore, PA AF Moore, Philip A., Jr. TI Development of a New Manure Amendment for Reducing Ammonia Volatilization and Phosphorus Runoff from Poultry Litter SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID ALUM-TREATED LITTER; ATMOSPHERIC AMMONIA; CHEMICAL AMENDMENTS; SALMONELLA POPULATIONS; XANES SPECTROSCOPY; BROILER-CHICKENS; SPECIATION; EMISSIONS; SULFATE; SOILS AB Treating poultry litter with alum is a best management practice that reduces phosphorus (P) runoff and ammonia (NH3) emissions. However, alum prices have increased substantially during the past decade. The goal of this research was to develop inexpensive manure amendments that are as effective as alum in reducing NH3 volatilization and P runoff. Sixteen amendments were developed using mixtures of alum mud, bauxite ore, sulfuric acid, liquid alum, and water. Alum mud is the residual left over from alum manufacture when produced by reacting bauxite with sulfuric acid. A laboratory NH3 volatilization study was conducted using 11 treatments: untreated poultry litter, poultry litter treated with liquid or dry alum, or eight new mixtures. All of the litter amendments tested resulted in significantly lower NH3 volatilization than untreated litter. Dry and liquid alum reduced NH3 losses by 86 and 75%, respectively. The eight new litter amendments reduced NH3 losses from 62 to 73% compared with untreated litter, which was not significantly different from liquid alum; the three most effective mixtures were not significantly different from dry alum. Water-extractable P (WEP) was significantly reduced by all of the amendments, three of which resulted in significantly lower WEP than dry alum. The most promising new amendments were mixtures of alum mud, bauxite, and sulfuric acid. The potential impact of these amendments could be enormous because they could be produced for less than half the price of alum while being as effective in reducing NH3 emissions and P runoff. C1 [Moore, Philip A., Jr.] USDA ARS, Poultry Prod & Prod Safety Res Unit, Plant Sci 115, Fayetteville, AR 72701 USA. RP Moore, PA (reprint author), USDA ARS, Poultry Prod & Prod Safety Res Unit, Plant Sci 115, Fayetteville, AR 72701 USA. EM philipm@uark.edu NR 71 TC 1 Z9 1 U1 13 U2 15 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2016 VL 45 IS 4 BP 1421 EP 1429 DI 10.2134/jeq2015.09.0483 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DP9ZJ UT WOS:000378856400035 PM 27380093 ER PT J AU Gonzalez, JM Smith, DR Livingston, S Warnemuende-Pappas, E Zwonitzer, M AF Gonzalez, Javier M. Smith, Douglas R. Livingston, Stan Warnemuende-Pappas, Elizabeth Zwonitzer, Martha TI Blind inlets: conservation practices to reduce herbicide losses from closed depressional areas SO JOURNAL OF SOILS AND SEDIMENTS LA English DT Article DE Blind inlet; Conservation practices; Pesticides; Tile riser; Water quality; Blind inlet ID FILTER STRIPS; ATRAZINE; SOIL; RUNOFF; METOLACHLOR; ADSORPTION; GLYPHOSATE; SORPTION; TRANSPORT; TILLAGE AB Purpose In a 6-year study, we investigated the effectiveness of blind inlets as a conservation practice in reducing pesticide losses compared to tile risers from two closed farmed depressional areas (potholes) in the US Midwest under a 4-year cropping rotation. Materials and methods In two adjacent potholes within the same farm and having similar soils, a conventional tile riser and blind inlet were installed. Each draining practice could be operated independent of each other in order to drain and monitor each depression with either practice. Sampling events (runoff events) were collected from the potholes from 2008 to 2013 using autosamplers. The samples were analyzed for atrazine, metolachlor, 2,4-D, glyphosate, and deethylatrazine. Results and discussion The results of this study demonstrated that the blind inlet reduced analyzed pesticide losses; however, the level of reduction was compound dependent: atrazine (57 %), 2,4-D (58 %), metolachlor (53 %), and glyphosate (11 %). Conclusions Results from this study corroborate previous research findings that blind inlets are an effective conservation practice to reduce discharge and pollutants, including pesticides from farmed pothole surface runoff in the US Midwest. C1 [Gonzalez, Javier M.; Livingston, Stan; Warnemuende-Pappas, Elizabeth] ARS, USDA, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. [Smith, Douglas R.] ARS, USDA, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Zwonitzer, Martha] Texas A&M AgriLife, Lubbock, TX 79403 USA. RP Gonzalez, JM (reprint author), ARS, USDA, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. EM Javier.gonzalez@ars.usda.gov NR 33 TC 0 Z9 0 U1 2 U2 4 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1439-0108 EI 1614-7480 J9 J SOIL SEDIMENT JI J. Soils Sediments PD JUL PY 2016 VL 16 IS 7 BP 1921 EP 1932 DI 10.1007/s11368-016-1362-0 PG 12 WC Environmental Sciences; Soil Science SC Environmental Sciences & Ecology; Agriculture GA DP5PZ UT WOS:000378551000008 ER PT J AU Kenar, JA AF Kenar, James A. TI Wonderful Legacy - New Beginnings SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Editorial Material C1 [Kenar, James A.] ARS, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ, Peoria, IL 61604 USA. RP Kenar, JA (reprint author), ARS, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ, Peoria, IL 61604 USA. EM jim.kenar@ars.usda.gov NR 1 TC 0 Z9 0 U1 2 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD JUL PY 2016 VL 93 IS 7 BP 883 EP 884 DI 10.1007/s11746-016-2858-x PG 2 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DQ0QG UT WOS:000378904400001 ER PT J AU Korlesky, NM Stolp, LJ Kodali, DR Goldschmidt, R Byrdwell, WC AF Korlesky, Nathan M. Stolp, Lucas J. Kodali, Dharma R. Goldschmidt, Robert Byrdwell, William C. TI Extraction and Characterization of Montmorency Sour Cherry (Prunus cerasus L.) Pit Oil SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Prunus cerasus L.; Sour cherry; Fatty acids; XRD; Triacylglycerol; Oxidative stability; Thermal stability; Eleostearic acid; Polymorphism ID IONIZATION MASS-SPECTROMETRY; ALPHA-ELEOSTEARIC ACID; LIQUID-CHROMATOGRAPHY; FATTY-ACIDS; TOCOPHEROL CONTENTS; DIETARY-SUPPLEMENT; KERNEL OILS; VITAMIN-D; SEED OIL; TRIACYLGLYCEROLS AB Montmorency sour cherry (Prunus cerasus L.) pit oil (CPO) was extracted and characterized by various methods including: GC, LC-MS, NMR, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and X-ray powder diffraction (XRD). The oil gave an acid value of 1.45 mg KOH/g, saponification value of 193 mg KOH/g and unsaponifiable matter content of 0.72 %. The oil contained oleic (O) and linoleic (l) acids as the major components with small concentrations of alpha-eleostearic acid (El, 9Z,11E,13E-octadecatrienoic acid) and saturated fatty acid palmitic (P) acid. The CPO contained six major triacyglycerols (TAG), OOO (16.83 %), OLO (16.64 %), LLO (13.20 %), OLP (7.25 %), OOP (6.49 %) and LElL (6.16 %) plus a number of other minor TAG. The TAG containing at least one saturated fatty acid constitute 33 % of the total. The polymorphic behavior of CPO as studied by DSC and XRD confirmed the presence of alpha, beta' and beta crystal forms. The oxidative induction time of CPO was 30.3 min at 130 A degrees C and the thermal decomposition temperature was 352 A degrees C. C1 [Korlesky, Nathan M.; Stolp, Lucas J.; Kodali, Dharma R.] Univ Minnesota, Dept Bioprod & Biosyst Engn, 2004 Folwell Ave, St Paul, MN 55108 USA. [Goldschmidt, Robert; Byrdwell, William C.] ARS, Food Composit & Methods Dev Lab, USDA, BHNRC, 10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Kodali, DR (reprint author), Univ Minnesota, Dept Bioprod & Biosyst Engn, 2004 Folwell Ave, St Paul, MN 55108 USA. EM dkodali@umn.edu FU University of Minnesota's Undergraduate Research Opportunity Program FX This research was partially funded by the University of Minnesota's Undergraduate Research Opportunity Program. We acknowledge Dr. Ramesh Avula for the shipment of sour cherry pits from Cherry Central of Michigan. NR 35 TC 0 Z9 0 U1 6 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD JUL PY 2016 VL 93 IS 7 BP 995 EP 1005 DI 10.1007/s11746-016-2835-4 PG 11 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DQ0QG UT WOS:000378904400013 ER PT J AU Knothe, G Razon, LF Madulid, DA Agoo, EMG de Castro, MEG AF Knothe, Gerhard Razon, Luis F. Madulid, Domingo A. Agoo, Esperanza Maribel G. de Castro, Maria Ellenita G. TI Fatty Acid Profiles of Some Fabaceae Seed Oils SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Centrosema pubescens; Crotalaria mucronata; Clitoria ternatea; Fabaceae; Fatty acid profile; Gas chromatography; Macroptilium lathyroides; Mass spectrometry; Pachyrhizus erosus; Senna alata ID COMPONENT ACIDS AB The fatty acid profiles of six seed oils of the Fabaceae (Leguminosae) family are reported and discussed. These are the seed oils of Centrosema pubescens, Clitoria ternatea, Crotalaria mucronata, Macroptilium lathyroides, Pachyrhizus erosus, and Senna alata. The most common fatty acid in the fatty acid profiles of these oils is linoleic acid with palmitic, stearic, oleic and linolenic acids usually completing the most prominent fatty acids in these species. Long-chain saturated fatty acids were observed in all oils. Centrosema pubescens and Macroptilium lathyroides exhibited the greatest amounts of long-chain saturated fatty acids exceeding the amount of stearic acid in these oils. C. pubescens exhibited slightly more that 6 % C24:0 together with some fatty acids > C25 and M. lathyroides approximately 4 % C22:0 and 3 % C24:0. The results are comparatively discussed to previous data on the fatty acid profiles of Fabaceae species. C1 [Knothe, Gerhard] ARS, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. [Razon, Luis F.] De La Salle Univ, Dept Chem Engn, 2401 Taft Ave, Manila 0922, Philippines. [Madulid, Domingo A.; Agoo, Esperanza Maribel G.; de Castro, Maria Ellenita G.] De La Salle Univ, Dept Biol, 2401 Taft Ave, Manila 0922, Philippines. RP Knothe, G (reprint author), ARS, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM gerhard.knothe@ars.usda.gov FU Philippine Higher Education Network of the Philippines' Commission on Higher Education (CHED-PhERNet) FX The authors thank the Philippine Higher Education Network of the Philippines' Commission on Higher Education (CHED-PhERNet) for a research grant and Kevin R. Steidley of USDA/ARS/NCAUR, for excellent technical assistance. NR 28 TC 1 Z9 1 U1 1 U2 4 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD JUL PY 2016 VL 93 IS 7 BP 1007 EP 1011 DI 10.1007/s11746-016-2845-2 PG 5 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DQ0QG UT WOS:000378904400014 ER PT J AU Stenfeldt, C Eschbaumer, M Rekant, SI Pacheco, JM Smoliga, GR Hartwig, EJ Rodriguez, LL Arzt, J AF Stenfeldt, Carolina Eschbaumer, Michael Rekant, Steven I. Pacheco, Juan M. Smoliga, George R. Hartwig, Ethan J. Rodriguez, Luis L. Arzt, Jonathan TI The Foot-and-Mouth Disease Carrier State Divergence in Cattle SO JOURNAL OF VIROLOGY LA English DT Article ID POLYMERASE CHAIN-REACTION; BUFFALO SYNCERUS CAFFER; KIDNEY-CELL LINE; VIRUS-INFECTION; VIRAL PATHWAYS; SECRETORY IGA; PATHOGENESIS; ANTIBODY; REPLICATION; PERSISTENCE AB The pathogenesis of persistent foot-and-mouth disease virus (FMDV) infection was investigated in 46 cattle that were either naive or had been vaccinated using a recombinant, adenovirus-vectored vaccine 2 weeks before challenge. The prevalence of FMDV persistence was similar in both groups (62% in vaccinated cattle, 67% in nonvaccinated cattle), despite vaccinated cattle having been protected from clinical disease. Analysis of antemortem infection dynamics demonstrated that the subclinical divergence between FMDV carriers and animals that cleared the infection had occurred by 10 days postinfection (dpi) in vaccinated cattle and by 21 dpi in nonvaccinated animals. The anatomic distribution of virus in subclinically infected, vaccinated cattle was restricted to the pharynx throughout both the early and the persistent phases of infection. In nonvaccinated cattle, systemically disseminated virus was cleared from peripheral sites by 10 dpi, while virus selectively persisted within the nasopharynx of a subset of animals. The quantities of viral RNA shed in oropharyngeal fluid during FMDV persistence were similar in vaccinated and nonvaccinated cattle. FMDV structural and nonstructural proteins were localized to follicle-associated epithelium of the dorsal soft palate and dorsal nasopharynx in persistently infected cattle. Host transcriptome analysis of tissue samples processed by laser capture microdissection indicated suppression of antiviral host factors (interferon regulatory factor 7, CXCL10 [gamma interferon-inducible protein 10], gamma interferon, and lambda interferon) in association with persistent FMDV. In contrast, during the transitional phase of infection, the level of expression of IFN-lambda mRNA was higher in follicle-associated epithelium of animals that had cleared the infection. This work provides novel insights into the intricate mechanisms of FMDV persistence and contributes to further understanding of this critical aspect of FMDV pathogenesis. C1 [Stenfeldt, Carolina; Eschbaumer, Michael; Rekant, Steven I.; Pacheco, Juan M.; Smoliga, George R.; Hartwig, Ethan J.; Rodriguez, Luis L.; Arzt, Jonathan] ARS, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, USDA, Greenport, NY 11944 USA. [Stenfeldt, Carolina; Eschbaumer, Michael; Rekant, Steven I.] Oak Ridge Inst Sci & Educ, PIADC Res Participat Program, Oak Ridge, TN 37830 USA. RP Stenfeldt, C; Arzt, J (reprint author), ARS, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, USDA, Greenport, NY 11944 USA.; Stenfeldt, C (reprint author), Oak Ridge Inst Sci & Educ, PIADC Res Participat Program, Oak Ridge, TN 37830 USA. EM Carolina.Stenfeldt@ars.usda.gov; Jonathan.Arzt@ars.usda.gov OI Pacheco, Juan/0000-0001-5477-0201; Arzt, Jonathan/0000-0002-7517-7893 FU USDA | Agricultural Research Service (ARS) (CRIS) [1940-32000-057-00D]; DHS | Science and Technology Directorate (ST) [HSHQPM-13-X-00131] FX This work, including the efforts of Juan M. Pacheco, George R. Smoliga, Ethan J. Hartwig, Luis Rodriguez, and Jonathan Arzt, was funded by USDA | Agricultural Research Service (ARS) (CRIS 1940-32000-057-00D). This work, including the efforts of Carolina Stenfeldt, Michael Eschbaumer, and Steven I. Rekant, was funded by DHS | Science and Technology Directorate (S&T) (HSHQPM-13-X-00131). NR 67 TC 2 Z9 2 U1 3 U2 4 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD JUL PY 2016 VL 90 IS 14 BP 6344 EP 6364 DI 10.1128/JVI.00388-16 PG 21 WC Virology SC Virology GA DP7DG UT WOS:000378658800015 PM 27147736 ER PT J AU Brown, P Juarez, B AF Brown, Patricia Juarez, Bernadette TI A word from USDA and OLAW SO LAB ANIMAL LA English DT Editorial Material C1 [Brown, Patricia] NIH, OLAW, OER, OD,NIH, Bldg 10, Bethesda, MD 20892 USA. [Juarez, Bernadette] APHIS, USDA, AC, Riverdale Pk, MD USA. RP Brown, P (reprint author), NIH, OLAW, OER, OD,NIH, Bldg 10, Bethesda, MD 20892 USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0093-7355 EI 1548-4475 J9 LAB ANIMAL JI Lab Anim. PD JUL PY 2016 VL 45 IS 7 BP 250 EP 250 DI 10.1038/laban.1058 PG 1 WC Veterinary Sciences SC Veterinary Sciences GA DP8OQ UT WOS:000378757900014 PM 27327010 ER PT J AU Durham, MJ AF Durham, Marion J. TI Women in Uniform SO NATURAL HISTORY LA English DT Biographical-Item C1 [Durham, Marion J.] NPS, Salt Lake City, UT USA. [Durham, Marion J.] US Forest Serv, Rocky Mt Forest & Range Expt Stn, Washington, DC USA. RP Durham, MJ (reprint author), US Forest Serv, Rocky Mt Forest & Range Expt Stn, Washington, DC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURAL HISTORY MAGAZINE PI NEW YORK PA 36 WEST 25TH STREET, FIFTH FLOOR, NEW YORK, NY 10010 USA SN 0028-0712 J9 NAT HIST JI Nat. Hist. PD JUL-AUG PY 2016 VL 124 IS 7 BP 37 EP 39 PG 3 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DP5ZF UT WOS:000378576000022 ER PT J AU Hufkens, K Keenan, TF Flanagan, LB Scott, RL Bernacchi, CJ Joo, E Brunsell, NA Verfaillie, J Richardson, AD AF Hufkens, Koen Keenan, Trevor F. Flanagan, Lawrence B. Scott, Russell L. Bernacchi, Carl J. Joo, Eva Brunsell, Nathaniel A. Verfaillie, Joseph Richardson, Andrew D. TI Productivity of North American grasslands is increased under future climate scenarios despite rising aridity SO NATURE CLIMATE CHANGE LA English DT Article ID DIGITAL REPEAT PHOTOGRAPHY; NET ECOSYSTEM PRODUCTIVITY; CARBON-DIOXIDE EXCHANGE; GREAT-PLAINS GRASSLAND; LEAF-AREA INDEX; UNITED-STATES; INTERANNUAL VARIABILITY; SEMIARID GRASSLAND; IMPROVED ALGORITHM; TALLGRASS PRAIRIE AB Grassland productivity is regulated by both temperature and the amount and timing of precipitation(1,2). Future climate change is therefore expected to influence grassland phenology and growth, with consequences for ecosystems and economies. However, the interacting effects of major shifts in temperature and precipitation on grasslands remain poorly understood and existing modelling approaches, although typically complex, do not extrapolate or generalize well and tend to disagree under future scenarios(3,4). Here we explore the potential responses of North American grasslands to climate change using a new, data-informed vegetation-hydrological model, a network of high-frequency ground observations across a wide range of grassland ecosystems and CMIP5 climate projections. Our results suggest widespread and consistent increases in vegetation fractional cover for the current range of grassland ecosystems throughout most of North America, despite the increase in aridity projected across most of our study area. Our analysis indicates a likely future shift of vegetation growth towards both earlier spring emergence and delayed autumn senescence, which would compensate for drought-induced reductions in summer fractional cover and productivity. However, because our model does not include the effects of rising atmospheric CO2 on photosynthesis and water use efficiency(5,6), climate change impacts on grassland productivity may be even larger than our results suggest. Increases in the productivity of North American grasslands over this coming century have implications for agriculture, carbon cycling and vegetation feedbacks to the atmosphere. C1 [Hufkens, Koen; Richardson, Andrew D.] Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA. [Keenan, Trevor F.] Macquarie Univ, Dept Biol Sci, Sydney, NSW 02109, Australia. [Flanagan, Lawrence B.] Univ Lethbridge, Dept Biol Sci, Lethbridge, AB T1K 3M4, Canada. [Scott, Russell L.] ARS, USDA, Southwest Watershed Res Ctr, Tucson, AZ 85719 USA. [Bernacchi, Carl J.; Joo, Eva] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Bernacchi, Carl J.] ARS, USDA, Global Change & Photosynthesis Res, Urbana, IL 61801 USA. [Brunsell, Nathaniel A.] Univ Kansas, Dept Geog & Atmospher Sci, Lawrence, KS 66045 USA. [Verfaillie, Joseph] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. RP Hufkens, K (reprint author), Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA. EM koen.hufkens@gmail.com; arichardson@oeb.harvard.edu RI Keenan, Trevor/B-2744-2010; OI Keenan, Trevor/0000-0002-3347-0258; Hufkens, Koen/0000-0002-5070-8109 FU NSF [EF-1065029]; Macquarie University; Natural Sciences and Engineering Research Council of Canada [RGPIN-2014-05882]; USDA NIFA [2012-02355]; National Science Foundation [IIA-1301789]; National Science Foundation-EPSCoR [NSF-0701906]; OpenDap; Montana Tech of the University of Montana; Stanford University; Carnegie Institution Department of Global Ecology; USDA-ARS; US Department of Energy; NSF Long Term Ecological Research Program (LTER) at the Konza Prairie Biological Station; LTER programme at the Konza Prairie Biological Station [DEB-0823341]; US Department of Energy [DE-AC02-05CH11231] FX The Richardson Lab acknowledges support from the NSF Macrosystems Biology programme (award EF-1065029). T.F.K. acknowledges support from a Macquarie University research fellowship. The Lethbridge ecosystem flux measurements were supported by Discovery grants from the Natural Sciences and Engineering Research Council of Canada to L.B.F. (RGPIN-2014-05882). We thank the World Climate Research Program's Working Group on Coupled Modelling, which is responsible for CMIP, and we thank the climate modelling groups (listed in Supplementary Table 6) for producing and making available their model output. For CMIP the US Department of Energy's Program for Climate Model Diagnosis and Inter-comparison provides coordinating support and led development of software infrastructure in partnership with the Global Organization for Earth System Science Portals. Furthermore, we acknowledge the Downscaled CMIP3 and CMIP5 Climate and Hydrology Projections archive hosted at http://gdo-dcp.ucllnl.org./downscaled_cmip_projections for making their data available. We thank X. Xiao for providing data from the Marena, Oklahoma, site acquired through research grants from the USDA NIFA (Project No. 2012-02355) and National Science Foundation (IIA-1301789). Research at the Continental Divide PhenoCam Site in Butte, Montana is supported by the National Science Foundation-EPSCoR (grant NSF-0701906), OpenDap and Montana Tech of the University of Montana. The Jasper Ridge Biological Preserve is supported by Stanford University and the Carnegie Institution Department of Global Ecology. Research at the Kendall site, Walnut Gulch Experimental Watershed, is funded by the USDA-ARS and the US Department of Energy. Data for PAB01 (Aboveground net primary productivity of tallgrass prairie based on accumulated plant biomass on core LTER watersheds) and climate data (AWE01, APT01) was supported by the NSF Long Term Ecological Research Program (LTER) at the Konza Prairie Biological Station. N.A.B. acknowledges support from the LTER programme at the Konza Prairie Biological Station (DEB-0823341), where the US-Kon Ameriflux site is sponsored by the US Department of Energy under a sub contract from DE-AC02-05CH11231. We thank T. Milliman for maintenance of the PhenoCam data archive, and our PhenoCam collaborators for their efforts in support of this project. NR 48 TC 5 Z9 5 U1 36 U2 49 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1758-678X EI 1758-6798 J9 NAT CLIM CHANGE JI Nat. Clim. Chang. PD JUL PY 2016 VL 6 IS 7 BP 710 EP + DI 10.1038/NCLIMATE2942 PG 7 WC Environmental Sciences; Environmental Studies; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DP6LP UT WOS:000378608900029 ER PT J AU Ebinger, MR Haroldson, MA van Manen, FT Costello, CM Bjornlie, DD Thompson, DJ Gunther, KA Fortin, JK Teisberg, JE Pils, SR White, PJ Cain, SL Cross, PC AF Ebinger, Michael R. Haroldson, Mark A. van Manen, Frank T. Costello, Cecily M. Bjornlie, Daniel D. Thompson, Daniel J. Gunther, Kerry A. Fortin, Jennifer K. Teisberg, Justin E. Pils, Shannon R. White, P. J. Cain, Steven L. Cross, Paul C. TI Detecting grizzly bear use of ungulate carcasses using global positioning system telemetry and activity data SO OECOLOGIA LA English DT Article DE Ursus arctos; GPS Cluster; Prediction; Multinomial model; Carcass visitation ID GREATER YELLOWSTONE ECOSYSTEM; AMERICAN BLACK BEARS; WHITEBARK-PINE; NATIONAL-PARK; BROWN BEARS; RESOURCE SELECTION; HABITAT SELECTION; URSUS-ARCTOS; MOOSE CALVES; KILL RATES AB Global positioning system (GPS) wildlife collars have revolutionized wildlife research. Studies of predation by free-ranging carnivores have particularly benefited from the application of location clustering algorithms to determine when and where predation events occur. These studies have changed our understanding of large carnivore behavior, but the gains have concentrated on obligate carnivores. Facultative carnivores, such as grizzly/brown bears (Ursus arctos), exhibit a variety of behaviors that can lead to the formation of GPS clusters. We combined clustering techniques with field site investigations of grizzly bear GPS locations (n = 732 site investigations; 2004-2011) to produce 174 GPS clusters where documented behavior was partitioned into five classes (large-biomass carcass, small-biomass carcass, old carcass, non-carcass activity, and resting). We used multinomial logistic regression to predict the probability of clusters belonging to each class. Two cross-validation methods-leaving out individual clusters, or leaving out individual bears-showed that correct prediction of bear visitation to large-biomass carcasses was 78-88 %, whereas the false-positive rate was 18-24 %. As a case study, we applied our predictive model to a GPS data set of 266 bear-years in the Greater Yellowstone Ecosystem (2002-2011) and examined trends in carcass visitation during fall hyperphagia (September-October). We identified 1997 spatial GPS clusters, of which 347 were predicted to be large-biomass carcasses. We used the clustered data to develop a carcass visitation index, which varied annually, but more than doubled during the study period. Our study demonstrates the effectiveness and utility of identifying GPS clusters associated with carcass visitation by a facultative carnivore. C1 [Ebinger, Michael R.] Univ Montana, Coll Forestry & Conservat, Univ Hall,Room 309, Missoula, MT 59812 USA. [Ebinger, Michael R.; Haroldson, Mark A.; van Manen, Frank T.; Cross, Paul C.] US Geol Survey, Northern Rocky Mt Sci Ctr, Interagency Grizzly Bear Study Team, 2327 Univ Way,Suite 2, Bozeman, MT 59715 USA. [Ebinger, Michael R.] Montana State Univ, Dept Ecol, Box 173460, Bozeman, MT 59717 USA. [Costello, Cecily M.] Montana Fish Wildlife & Parks, 490 N Meridian Rd, Kalispell, MT 59901 USA. [Bjornlie, Daniel D.; Thompson, Daniel J.] Wyoming Game & Fish Dept, Large Carnivore Sect, 260 Buena Vista, Lander, WY 82520 USA. [Gunther, Kerry A.] Natl Pk Serv, Yellowstone Ctr Resources, Bear Management Off, POB 168, Yellowstone Natl Pk, WY 82190 USA. [Fortin, Jennifer K.; Teisberg, Justin E.] Washington State Univ, Sch Biol Sci, POB 644236, Pullman, WA 99164 USA. [Teisberg, Justin E.] US Fish & Wildlife Serv, Grizzly Bear Recovery Program, Libby, MT 59923 USA. [Pils, Shannon R.] US Forest Serv, Shoshone Natl Forest, 203A Yellowstone Ave, Cody, WY 82414 USA. [White, P. J.] Natl Pk Serv, Yellowstone Ctr Resources, POB 168, Yellowstone Natl Pk, WY 82190 USA. [Cain, Steven L.] Grand Teton Natl Pk, POB 170, Moose, WY 83012 USA. [Cain, Steven L.] Grand Teton Natl Pk Fdn, POB 249, Moose, WY 83012 USA. RP Ebinger, MR (reprint author), Univ Montana, Coll Forestry & Conservat, Univ Hall,Room 309, Missoula, MT 59812 USA.; Ebinger, MR (reprint author), US Geol Survey, Northern Rocky Mt Sci Ctr, Interagency Grizzly Bear Study Team, 2327 Univ Way,Suite 2, Bozeman, MT 59715 USA.; Ebinger, MR (reprint author), Montana State Univ, Dept Ecol, Box 173460, Bozeman, MT 59717 USA. EM mebinger@usgs.gov FU National Park Service, US Fish and Wildlife Service; US Geological survey FX This study was funded in part by the National Park Service, US Fish and Wildlife Service, and the US Geological survey. NR 84 TC 2 Z9 2 U1 12 U2 22 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 EI 1432-1939 J9 OECOLOGIA JI Oecologia PD JUL PY 2016 VL 181 IS 3 BP 695 EP 708 DI 10.1007/s00442-016-3594-5 PG 14 WC Ecology SC Environmental Sciences & Ecology GA DP8ZY UT WOS:000378787700006 PM 26971522 ER PT J AU Long, RA Bowyer, RT Porter, WP Mathewson, P Monteith, KL Findholt, SL Dick, BL Kie, JG AF Long, Ryan A. Bowyer, R. T. Porter, Warren P. Mathewson, Paul Monteith, Kevin L. Findholt, Scott L. Dick, Brian L. Kie, John G. TI Linking habitat selection to fitness-related traits in herbivores: the role of the energy landscape SO OECOLOGIA LA English DT Article DE Behavior; Biophysics; Energetics; Thermoregulation; Ingesta-free body fat ID VAGINAL-IMPLANT TRANSMITTERS; YELLOWSTONE-NATIONAL-PARK; ROCKY-MOUNTAIN ELK; NORTH-AMERICAN ELK; RESOURCE SELECTION; MULE DEER; POPULATION-DYNAMICS; TAILED DEER; BODY-MASS; RED DEER AB Animals may partially overcome environmental constraints on fitness by behaviorally adjusting their exposure to costs and supplies of energy. Few studies, however, have linked spatiotemporal variation in the energy landscape to behaviorally mediated measures of performance that ostensibly influence individual fitness. We hypothesized that strength of selection by North American elk (Cervus elaphus) for areas that reduced costs of thermoregulation and activity, and increased access to high-quality forage, would influence four energetically mediated traits related to fitness: birth mass of young, nutritional condition of adult females at the onset of winter, change in nutritional condition of females between spring and winter, and neonatal survival. We used a biophysical model to map spatiotemporally explicit costs of thermoregulation and activity experienced by elk in a heterogeneous landscape. We then combined model predictions with data on forage characteristics, animal locations, nutritional condition, and mass and survival of young to evaluate behaviorally mediated effects of the energy landscape on fitness-related traits. During spring, when high-quality forage was abundant, female elk that consistently selected low-cost areas before parturition gave birth to larger young than less-selective individuals, and birth mass had a strong, positive influence on probability of survival. As forage quality declined during autumn, however, lactating females that consistently selected the highest quality forage available accrued more fat and entered winter in better condition than less-selective individuals. Results of our study highlight the importance of understanding the dynamic nature of energy landscapes experienced by free-ranging animals. C1 [Long, Ryan A.; Bowyer, R. T.; Kie, John G.] Idaho State Univ, Dept Biol Sci, Pocatello, ID 83209 USA. [Porter, Warren P.; Mathewson, Paul] Univ Wisconsin Madison, Dept Zool, Madison, WI 53796 USA. [Monteith, Kevin L.] Univ Wyoming, Dept Zool & Physiol, Wyoming Cooperat Fish & Wildlife Res Unit, Laramie, WY 82071 USA. [Findholt, Scott L.] Oregon Dept Fish & Wildlife, La Grande, OR 97850 USA. [Dick, Brian L.] US Forest Serv, Pacific NW Res Stn, La Grande, OR 97850 USA. [Long, Ryan A.] Univ Idaho, Dept Fish & Wildlife Sci, Moscow, ID 83844 USA. RP Long, RA (reprint author), Idaho State Univ, Dept Biol Sci, Pocatello, ID 83209 USA.; Long, RA (reprint author), Univ Idaho, Dept Fish & Wildlife Sci, Moscow, ID 83844 USA. EM ralong@uidaho.edu FU US Forest Service; Shikar Safari Club; Idaho State University; STAR Fellowship from the Environmental Protection Agency; GK-12 Fellowship from the National Science Foundation; American Society of Mammalogists Fellowship in Mammalogy FX This research was funded by the US Forest Service, the Shikar Safari Club, and Idaho State University. R. Long was supported by a STAR Fellowship from the Environmental Protection Agency, a GK-12 Fellowship from the National Science Foundation, and the American Society of Mammalogists Fellowship in Mammalogy. We are indebted to R. Kennedy, D. Rea, and M. Vavra of the US Forest Service, B. Johnson of the Oregon Department of Fish and Wildlife, and K. Stewart, C. McKee, D. Walsh, and J. Zweifel of the University of Nevada Reno for providing substantial logistical support during the study. We thank K. Aho for assistance with statistical analyses. W. Porter is an author of Niche Mapper, patented software that may be purchased for independent research or commercial use. The authors declare that they have no conflict of interest. NR 68 TC 2 Z9 2 U1 21 U2 41 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 EI 1432-1939 J9 OECOLOGIA JI Oecologia PD JUL PY 2016 VL 181 IS 3 BP 709 EP 720 DI 10.1007/s00442-016-3604-7 PG 12 WC Ecology SC Environmental Sciences & Ecology GA DP8ZY UT WOS:000378787700007 PM 27003702 ER PT J AU Gregory, JA Shepley-McTaggart, A Umpierrez, M Hurlburt, BK Maleki, SJ Sampson, HA Mayfield, SP Berin, MC AF Gregory, James A. Shepley-McTaggart, Ariel Umpierrez, Michelle Hurlburt, Barry K. Maleki, Soheila J. Sampson, Hugh A. Mayfield, Stephen P. Berin, M. Cecilia TI Immunotherapy using algal-produced Ara h 1 core domain suppresses peanut allergy in mice SO PLANT BIOTECHNOLOGY JOURNAL LA English DT Article DE algae; peanut; allergy; immunotherapy; biotechnology; recombinant protein; Chlamydomonas reinhardtii ID IGE-BINDING EPITOPES; BET V 1; ORAL IMMUNOTHERAPY; MAST-CELLS; FOOD ALLERGY; MURINE MODEL; CHLAMYDOMONAS-REINHARDTII; RECOMBINANT ALLERGENS; INDUCED ANAPHYLAXIS; MUTATIONAL ANALYSIS AB Peanut allergy is an IgE-mediated adverse reaction to a subset of proteins found in peanuts. Immunotherapy aims to desensitize allergic patients through repeated and escalating exposures for several months to years using extracts or flours. The complex mix of proteins and variability between preparations complicates immunotherapy studies. Moreover, peanut immunotherapy is associated with frequent negative side effects and patients are often at risk of allergic reactions once immunotherapy is discontinued. Allergen-specific approaches using recombinant proteins are an attractive alternative because they allow more precise dosing and the opportunity to engineer proteins with improved safety profiles. We tested whether Ara h 1 and Ara h 2, two major peanut allergens, could be produced using chloroplast of the unicellular eukaryotic alga, Chlamydomonas reinhardtii. C. reinhardtii is novel host for producing allergens that is genetically tractable, inexpensive and easy to grow, and is able to produce more complex proteins than bacterial hosts. Compared to the native proteins, algal-produced Ara h 1 core domain and Ara h 2 have a reduced affinity for IgE from peanut-allergic patients. We further found that immunotherapy using algal-produced Ara h 1 core domain confers protection from peanut-induced anaphylaxis in a murine model of peanut allergy. C1 [Gregory, James A.; Shepley-McTaggart, Ariel; Umpierrez, Michelle; Sampson, Hugh A.; Berin, M. Cecilia] Icahn Sch Med Mt Sinai, Dept Pediat, New York, NY 10029 USA. [Gregory, James A.; Shepley-McTaggart, Ariel; Umpierrez, Michelle; Sampson, Hugh A.; Berin, M. Cecilia] Icahn Sch Med Mt Sinai, Inst Immunol, New York, NY 10029 USA. [Hurlburt, Barry K.; Maleki, Soheila J.] ARS, USDA, So Reg Res Ctr, New Orleans, LA USA. [Sampson, Hugh A.; Berin, M. Cecilia] Icahn Sch Med Mt Sinai, Mindich Child Hlth & Dev Inst, New York, NY 10029 USA. [Mayfield, Stephen P.] Univ Calif San Diego, Dept Biol, La Jolla, CA USA. [Berin, M. Cecilia] Icahn Sch Med Mt Sinai, Tisch Canc Inst, New York, NY 10029 USA. RP Gregory, JA (reprint author), Icahn Sch Med Mt Sinai, Dept Pediat, New York, NY 10029 USA.; Gregory, JA (reprint author), Icahn Sch Med Mt Sinai, Inst Immunol, New York, NY 10029 USA. EM james.alan.gregory@gmail.com FU David H. and Julia Koch Research Program in Food Allergy Therapeutics, the National Science Foundation [CBET-1160184] FX This work was supported by grants from the David H. and Julia Koch Research Program in Food Allergy Therapeutics, the National Science Foundation (CBET-1160184). SPM is a founder of Triton Health and Nutrition and has an equity position in that company, which could benefit from this research. NR 57 TC 0 Z9 0 U1 6 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1467-7644 EI 1467-7652 J9 PLANT BIOTECHNOL J JI Plant Biotechnol. J. PD JUL PY 2016 VL 14 IS 7 BP 1541 EP 1550 DI 10.1111/pbi.12515 PG 10 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA DP8II UT WOS:000378741200006 PM 26801740 ER PT J AU Wu, X Li, YX Shi, YS Song, YC Zhang, DF Li, CH Buckler, ES Li, Y Zhang, ZW Wang, TY AF Wu, Xun Li, Yongxiang Shi, Yunsu Song, Yanchun Zhang, Dengfeng Li, Chunhui Buckler, Edward S. Li, Yu Zhang, Zhiwu Wang, Tianyu TI Joint-linkage mapping and GWAS reveal extensive genetic loci that regulate male inflorescence size in maize SO PLANT BIOTECHNOLOGY JOURNAL LA English DT Article DE maize; male inflorescence size; joint-linkage; GWAS; QTLs; candidate gene ID GENOME-WIDE ASSOCIATION; QUANTITATIVE TRAIT LOCI; ZINC-FINGER PROTEIN; TRANSCRIPTION FACTOR; ARABIDOPSIS-THALIANA; TASSEL TRAITS; ARCHITECTURE; POPULATION; ENCODES; LEAF AB Both insufficient and excessive male inflorescence size leads to a reduction in maize yield. Knowledge of the genetic architecture of male inflorescence is essential to achieve the optimum inflorescence size for maize breeding. In this study, we used approximately eight thousand inbreds, including both linkage populations and association populations, to dissect the genetic architecture of male inflorescence. The linkage populations include 25 families developed in the U.S. and 11 families developed in China. Each family contains approximately 200 recombinant inbred lines (RILs). The association populations include approximately 1000 diverse lines from the U.S. and China. All inbreds were genotyped by either sequencing or microarray. Inflorescence size was measured as the tassel primary branch number (TBN) and tassel length (TL). A total of 125 quantitative trait loci (QTLs) were identified (63 for TBN, 62 for TL) through linkage analyses. In addition, 965 quantitative trait nucleotides (QTNs) were identified through genomewide study (GWAS) at a bootstrap posterior probability (BPP) above a 5% threshold. These QTLs/QTNs include 24 known genes that were cloned using mutants, for example Ramosa3 (ra3), Thick tassel dwarf1 (td1), tasselseed2 (ts2), liguleless2 (lg2), ramosa1 (ra1), barren stalk1 (ba1), branch silkless1 (bd1) and tasselseed6 (ts6). The newly identified genes encode a zinc transporter (e.g. GRMZM5G838098 and GRMZM2G047762), the adapt in terminal region protein (e.g. GRMZM5G885628), O-methyl-transferase (e.g. GRMZM2G147491), helix-loop-helix (HLH) DNA-binding proteins (e.g. GRMZM2G414252 and GRMZM2G042895) and an SBP-box protein (e.g. GRMZM2G058588). These results provide extensive genetic information to dissect the genetic architecture of inflorescence size for the improvement of maize yield. C1 [Wu, Xun; Li, Yongxiang; Shi, Yunsu; Song, Yanchun; Zhang, Dengfeng; Li, Chunhui; Li, Yu; Wang, Tianyu] Chinese Acad Agr Sci, Inst Crop Sci, Beijing, Peoples R China. [Buckler, Edward S.] Cornell Univ, Inst Genom Divers, Ithaca, NY 14850 USA. [Buckler, Edward S.] ARS, USDA, Ithaca, NY 14853 USA. [Zhang, Zhiwu] Northeast Agr Univ, Dept Agron, Harbin, Heilongjiang, Peoples R China. [Wu, Xun] Nanchong Acad Agr Sci, Nanchong, Sichuan, Peoples R China. [Zhang, Zhiwu] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. RP Li, Y; Wang, TY (reprint author), Chinese Acad Agr Sci, Inst Crop Sci, Beijing, Peoples R China.; Buckler, ES (reprint author), Cornell Univ, Inst Genom Divers, Ithaca, NY 14850 USA.; Buckler, ES (reprint author), ARS, USDA, Ithaca, NY 14853 USA.; Zhang, ZW (reprint author), Northeast Agr Univ, Dept Agron, Harbin, Heilongjiang, Peoples R China.; Zhang, ZW (reprint author), Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. EM esb33@cornell.edu; liyu03@caas.cn; zhiwu.zhang@wsu.edu; wangtianyu@caas.cn OI Buckler, Edward/0000-0002-3100-371X FU National Natural Science Foundation [91335206]; Ministry of Science and Technology of China [2014CB138200, 2011DFA30450, 2013BAD01B02]; CAAS Innovation Program; US NSF [0820619, 1238014]; USDA-ARS; China Postdoctoral Sciences Foundation [2014M550901] FX This research was supported by the National Natural Science Foundation (91335206), the Ministry of Science and Technology of China (2014CB138200, 2011DFA30450, 2013BAD01B02), the CAAS Innovation Program, the US NSF #0820619, #1238014, the USDA-ARS, and the China Postdoctoral Sciences Foundation (2014M550901). NR 66 TC 1 Z9 1 U1 9 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1467-7644 EI 1467-7652 J9 PLANT BIOTECHNOL J JI Plant Biotechnol. J. PD JUL PY 2016 VL 14 IS 7 BP 1551 EP 1562 DI 10.1111/pbi.12519 PG 12 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA DP8II UT WOS:000378741200007 PM 26801971 ER PT J AU Rosser, TG Griffin, MJ Quiniou, SMA Alberson, NR Woodyard, ET Mischke, CC Greenway, TE Wise, DJ Pote, LM AF Rosser, Thomas G. Griffin, Matt J. Quiniou, Sylvie M. A. Alberson, Neely R. Woodyard, Ethan T. Mischke, Charles C. Greenway, Terrence E. Wise, David J. Pote, Linda M. TI Myxobolus ictiobus n. sp and Myxobolus minutus n. sp (Cnidaria: Myxobolidae) from the gills of the smallmouth buffalo Ictiobus bubalus Rafinesque (Cypriniformes: Catostomidae) SO SYSTEMATIC PARASITOLOGY LA English DT Article ID CHANNEL CATFISH; SALMONID FISHES; 1882 MYXOZOA; LIFE-CYCLE; MYXOSPOREA; DISEASE; DNA; SEQUENCES; SYNOPSIS; BUTSCHLI AB The smallmouth buffalo Ictiobus bubalus Rafinesque (Catostomidae) is native to North American waterways and occasionally grown in pond aquaculture. Species of Myxobolus Butschli, 1882 have been reported from the gills, integument, and intestinal tract of buffalo fish, although there is ambiguity in some host records. In the summer of 2013, thirteen adult smallmouth buffalo were seined from a 0.1-acre (0.04-hectare) experimental research pond at the Thad Cochran National Warmwater Aquaculture Center in Stoneville, Mississippi, USA, and examined for the presence of parasitic infection. Two previously unknown species of Myxobolus were observed parasitising the gills. Plasmodia of the two species differed from each other in both size and shape. Morphologically the two species were distinct from one another and from other Myxobolus spp. previously reported from buffalo fish. Myxospores of Myxobolus ictiobus n. sp. were spherical and measured 12.7-14.5 (13.9 +/- 0.4) A mu m in length and 10.7-13.6 (12.5 +/- 0.7) A mu m in width with a thickness of 10.3-14.8 (12.6 +/- 2.3) A mu m. Polar capsules measured 5.6-7.4 (6.6 +/- 0.4) A mu m in length and 3.7-4.9 (4.5 +/- 0.8) A mu m in width and each contained a coiled polar filament with 5-6 turns. Myxospores of Myxobolus minutus n. sp. were circular in shape and measured 7.4-9.6 (8.6 +/- 0.7) A mu m in length and 7.5-9.9 (8.8 +/- 0.7) A mu m in width with a thickness of 6.5-7.3 (6.7 +/- 0.3) A mu m. Polar capsules measured 3.6-4.9 (4.3 +/- 0.3) A mu m in length and 2.8-3.8 (3.3 +/- 0.3) A mu m and each contained a coiled polar filament with 5-6 turns. Supplemental 18S rRNA gene sequencing identified unique sequences for each isolate. Phylogenetic analysis of 18S rRNA sequences demonstrated a strong clustering of both isolates with other species of Myxobolus from cypriniform fish. C1 [Rosser, Thomas G.; Alberson, Neely R.; Woodyard, Ethan T.; Pote, Linda M.] Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA. [Griffin, Matt J.] Mississippi State Univ, Dept Pathobiol & Populat Med, Coll Vet Med, Mississippi State, MS 39762 USA. [Griffin, Matt J.; Mischke, Charles C.; Greenway, Terrence E.; Wise, David J.] Mississippi State Univ, Thad Cochran Natl Warmwater Aquaculture Ctr, Delta Res & Extens Ctr, Aquat Res & Diagnost Lab, Stoneville, MS 38776 USA. [Quiniou, Sylvie M. A.] USDA ARS, Warmwater Aquaculture Res Unit, Stoneville, MS 38776 USA. [Mischke, Charles C.; Greenway, Terrence E.; Wise, David J.] Mississippi State Univ, Mississippi Agr & Forestry Expt Stn, Mississippi State, MS 39762 USA. RP Griffin, MJ (reprint author), Mississippi State Univ, Dept Pathobiol & Populat Med, Coll Vet Med, Mississippi State, MS 39762 USA.; Griffin, MJ (reprint author), Mississippi State Univ, Thad Cochran Natl Warmwater Aquaculture Ctr, Delta Res & Extens Ctr, Aquat Res & Diagnost Lab, Stoneville, MS 38776 USA. EM matt.griffin@msstate.edu FU National Institute of Food and Agriculture, U.S. Department of Agriculture [MIS-371530]; U.S. Department of Agriculture, Agricultural Research Service-Catfish Health Initiative; Mississippi State University College of Veterinary Medicine; Mississippi Agriculture and Forestry Experiment Station (MAFES) FX This work was supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, Project Number MIS-371530, the U.S. Department of Agriculture, Agricultural Research Service-Catfish Health Initiative, the Mississippi State University College of Veterinary Medicine, and the Mississippi Agriculture and Forestry Experiment Station (MAFES). This is a MAFES publication, J-12756. NR 37 TC 0 Z9 0 U1 2 U2 3 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0165-5752 EI 1573-5192 J9 SYST PARASITOL JI Syst. Parasitol. PD JUL PY 2016 VL 93 IS 6 BP 565 EP 574 DI 10.1007/s11230-016-9636-x PG 10 WC Parasitology SC Parasitology GA DP8XU UT WOS:000378782100004 PM 27307169 ER PT J AU Killmaster, LF Levin, ML AF Killmaster, Lindsay F. Levin, Michael L. TI Isolation and Short-Term Persistence of Ehrlichia ewingii in Cell Culture SO VECTOR-BORNE AND ZOONOTIC DISEASES LA English DT Article DE Cell culture; Ehrlichia ewingii; In vitro propagation ID AMBLYOMMA-AMERICANUM ACARI; GRANULOCYTIC EHRLICHIOSIS; PREVALENCE; AGENT; IXODIDAE; TRANSMISSION; CHAFFEENSIS; USA AB Ehrlichia ewingii is the causative agent of human and canine granulocytic ehrlichiosis. Since its discovery in 1970, little work has been done to characterize the pathogen or study the transmission dynamics due to the inability to grow the agent in vitro. The aim of this study was to assess the suitability of multiple cell lines and media formulations for propagation of E. ewingii in cell culture. In this study, we present an overview of attempts to isolate E. ewingii from the buffy coat of goats naturally infected by Amblyomma americanum ticks, as well as a methodology for maintaining the pathogen for up to 16 weeks in culture. The most promising results were seen with HL-60 cells differentiated by the addition of 1.5% DMSO to the media and supplemented with 8mM l-glutamine. Cultures were passaged multiple times, and fluorescence and morulae were observed by indirect fluorescent antibody test and Diff-Quik staining. It is our hope that this information will provide a foundation for future attempts to propagate and maintain E. ewingii in cell culture. C1 [Killmaster, Lindsay F.; Levin, Michael L.] Ctr Dis Control & Prevent, Rickettsial Zoonoses Branch, US Dept HHS, 1600 Clifton Rd,MS G-13, Atlanta, GA 30333 USA. [Killmaster, Lindsay F.] ARS, SE Poultry Res Lab, USDA, Athens, GA USA. RP Levin, ML (reprint author), Ctr Dis Control & Prevent, Rickettsial Zoonoses Branch, US Dept HHS, 1600 Clifton Rd,MS G-13, Atlanta, GA 30333 USA. EM mlevin@cdc.gov NR 19 TC 0 Z9 0 U1 3 U2 3 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1530-3667 EI 1557-7759 J9 VECTOR-BORNE ZOONOT JI Vector-Borne Zoonotic Dis. PD JUL PY 2016 VL 16 IS 7 BP 445 EP 448 DI 10.1089/vbz.2015.1938 PG 4 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA DP7BL UT WOS:000378653900002 PM 27228133 ER PT J AU Powell, EJ Cunnick, JE Knetter, SM Loving, CL Waide, EH Dekkers, JCM Tuggle, CK AF Powell, Ellis J. Cunnick, Joan E. Knetter, Susan M. Loving, Crystal L. Waide, Emily H. Dekkers, Jack C. M. Tuggle, Christopher K. TI NK cells are intrinsically functional in pigs with Severe Combined Immunodeficiency (SCID) caused by spontaneous mutations in the Artemis gene SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article DE Natural killer; NK cells; SCID; Pig; Innate immunity; Artemis; Perforin ID NATURAL-KILLER-CELLS; IFN-GAMMA PRODUCTION; PERFORIN EXPRESSION; T-CELLS; IL-2; CYTOTOXICITY; DEFICIENCY; PHENOTYPE; IMMUNITY AB We have identified Severe Combined Immunodeficiency (SCID) in a line of Yorkshire pigs at Iowa State University. These SCID pigs lack B-cells and T-cells, but possess Natural Killer (NK) cells. This SCID phenotype is caused by recessive mutations in the Artemis gene. Interestingly, two human tumor cell lines, PANC-1 and A375-SM, survived after injection into these SCID pigs, but, as we demonstrate here, these cells, as well as K562 tumor cells, can be lysed in vitro by NK cells from SCID and non-SCID pigs. NK cells from both SCID and non-SCID pigs required activation in vitro with either recombinant human IL-2 or the combination of recombinant porcine IL-12 and IL-18 to kill tumor targets. We also showed that SCID NK cells could be activated to produce perforin, and perforin production was greatly enhanced in NK cells from both SCID and non-SCID pigs after IL-2 cytokine treatment. While CD16+, CD172- NK cells constituted an average of only 4% in non-SCID pigs, NK cells averaged 27% of the peripheral blood mononuclear cell population in SCID pigs. We found no significant differences in killing activity per NK cell between SCID and non-SCID pigs. We conclude that survival of human cancer cells in these SOD pigs is not due to an intrinsic defect in NK cell killing ability. (C) 2016 Elsevier B.V. All rights reserved. C1 [Powell, Ellis J.; Cunnick, Joan E.; Knetter, Susan M.; Waide, Emily H.; Dekkers, Jack C. M.; Tuggle, Christopher K.] Iowa State Univ, Dept Anim Sci, Ames, IA USA. [Loving, Crystal L.] USDA ARS, Natl Anim Dis Ctr, Food Safety & Enter Pathogens Res Unit, Washington, DC 20250 USA. RP Tuggle, CK (reprint author), Iowa State Univ, Dept Anim Sci, Ames, IA USA. EM cktuggle@iastate.edu FU Iowa State University Office of Vice President for Research; USDA National Needs Fellowship Grant [2012-38420-19286]; NIH [1R24OD019813-01] FX This work was funded by Iowa State University Office of Vice President for Research, and grant 1R24OD019813-01 from NIH. EJP gratefully acknowledges support from a USDA National Needs Fellowship Grant (2012-38420-19286). We thank the Lauren Christian Swine Breeding farm staff and Iowa State University Laboratory Animal Research staff for excellent professional care of the SCID pigs. We thank Dr. Deryl Troyer for the A375-SM and PANC-1 cell lines, and give tremendous thanks to Dr. Martine Schroyen, the ISU Cytometry Flow Facility, and Dr. Shawn Rigby for all the help and advice. We also thank Sam Humphrey for assistance with flow cytometric software. NR 29 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 EI 1873-2534 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD JUL PY 2016 VL 175 BP 1 EP 6 DI 10.1016/j.vetimm.2016.04.008 PG 6 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA DP7IA UT WOS:000378671200001 PM 27269786 ER PT J AU Pandarangga, P Brown, CC Miller, PJ Haddas, R Rehmani, SF Afonso, CL Susta, L AF Pandarangga, P. Brown, C. C. Miller, P. J. Haddas, R. Rehmani, S. F. Afonso, C. L. Susta, L. TI Pathogenesis of New Strains of Newcastle Disease Virus From Israel and Pakistan SO VETERINARY PATHOLOGY LA English DT Article DE avian paramyxovirus serotype 1; Newcastle disease virus; velogenic viscerotropic NDV; chickens; pathogenesis; pathogenicity; subgenotype VIIi; subgenotype XIIIb; fifth NDV panzootic ID GENOTYPES AB In the past few years, Newcastle disease virus (NDV) strains with epizootic characteristics belonging to subgenotypes VIIi and XIIIb emerged in the Middle East and Asia. In this study, 2 NDV strains1 representative of subgenotype VIIi isolated in Israel (Kvuzat/13) and 1 representative of subgenotype XIIIb isolated in Pakistan (Karachi/07)were characterized by intracerebral pathogenicity index and detailed clinicopathologic assessment. The intracerebral pathogenicity index values for Kvuzat/13 and Karachi/07 were 1.89 and 1.85, respectively, classifying these strains as virulent by international standards. In 4-week-old White Leghorn chickens, both strains caused 100% mortality within 4 (Kvuzat/13) and 5 (Karachi/07) days postinfection. Histopathology and immunohistochemistry for NDV nucleoprotein showed that both strains had wide systemic distribution, especially targeting lymphoid organs and mucosa-associated lymphoid tissues in the respiratory and intestinal tracts. Results of the animal experiment confirm that both Kvuzat/13 and Karachi/07 are highly virulent and behaved as velogenic viscerotropic NDV strains. C1 [Pandarangga, P.] Nusa Cendana Univ, Dept Vet Pathol, Kupang, Indonesia. [Pandarangga, P.; Brown, C. C.] Univ Georgia, Coll Vet Med, Dept Vet Pathol, Athens, GA USA. [Miller, P. J.; Afonso, C. L.; Susta, L.] ARS, Southeast Poultry Res Lab, USDA, Athens, GA USA. [Haddas, R.] Kimron Vet Inst, Bet Dagan, Israel. [Rehmani, S. F.] Univ Vet & Anim Sci, Lahore, Pakistan. [Susta, L.] Univ Guelph, Ontario Vet Coll, Dept Pathobiol, Guelph, ON, Canada. RP Susta, L (reprint author), Univ Guelph, Dept Pathobiol, Guelph, ON N1G 2W1, Canada. EM lsusta@uoguelph.ca FU USDA-ARS CRIS [6040-32000-064] FX The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This research was supported by USDA-ARS CRIS 6040-32000-064. NR 10 TC 0 Z9 0 U1 2 U2 3 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0300-9858 EI 1544-2217 J9 VET PATHOL JI Vet. Pathol. PD JUL PY 2016 VL 53 IS 4 BP 792 EP 796 DI 10.1177/0300985815622972 PG 5 WC Pathology; Veterinary Sciences SC Pathology; Veterinary Sciences GA DP7XE UT WOS:000378711700012 PM 26940837 ER PT J AU Callahan, MT Micallef, SA Sharma, M Millner, PD Buchanan, RL AF Callahan, Mary Theresa Micallef, Shirley A. Sharma, Manan Millner, Patricia D. Buchanan, Robert L. TI Metrics Proposed To Prevent the Harvest of Leafy Green Crops Exposed to Floodwater Contaminated with Escherichia coli SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID ENTERICA SEROVAR TYPHIMURIUM; MICROBIAL SAFETY; WASTE-WATER; SOIL; SALMONELLA; SURVIVAL; TRANSPORT; MANURE; PREVALENCE; BACTERIA AB The California Leafy Green Products Handler Marketing Agreement (LGMA) requires leafy green crops within 9 m of the edge of a flooded field not be harvested due to potential contamination (California Leafy Green Products Handler Marketing Board, Commodity Specific Flood Safety Guidelines for the Production and Harvest of Lettuce and Leafy Greens, 2012). Further, previously flooded soils should not be replanted for 60 days. In this study, the suitability of the LGMA metrics for farms in the Mid-Atlantic region of the United States was evaluated. The upper end of a spinach bed (in Beltsville, MD) established on a -5% grade was flooded with water containing 6 log CFU/ml Escherichia coli to model a worst-case scenario of bacterial movement through soil. Escherichia coli prevalence in soil and on foliar tissue was determined by most probable number (MPN) analysis at distances up to 9 m from the edge of the flood for 63 days. While E. coli was quickly detected at the 9-m distance within 1 day in the spring trial and within 3 days in the fall trial, no E. coli was detected on plants outside the flood zone after 14 days. On day 63 for the two trials, E. coli populations in the flood zone soil were higher in the fall than in the spring. Regression analysis predicted that the time required for a 3-log MPN/g (dry weight) decrease in E. coli populations inside the flood zone was within the 60-day LGMA guideline in the spring but would require 90 days in the fall. Overall, data suggest that the current guidelines should be revised to include considerations of field and weather conditions that may promote bacterial movement and survival. C1 [Callahan, Mary Theresa; Buchanan, Robert L.] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Callahan, Mary Theresa; Micallef, Shirley A.] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA. [Micallef, Shirley A.; Buchanan, Robert L.] Univ Maryland, Ctr Food Safety & Secur Syst, College Pk, MD 20742 USA. [Sharma, Manan; Millner, Patricia D.] USDA, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. RP Buchanan, RL (reprint author), Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA.; Buchanan, RL (reprint author), Univ Maryland, Ctr Food Safety & Secur Syst, College Pk, MD 20742 USA. EM rbuchanan@umd.edu FU USDA \ National Institute of Food and Agriculture (NIFA) [2011-51181-30767]; USDA \ Agricultural Research Service (ARS) [8042-32420-005-00] FX This work, including the efforts of Mary Theresa Callahan, Shirley A. Micallef, Manan Sharma, Patricia Millner, and Robert L. Buchanan, was funded by USDA vertical bar National Institute of Food and Agriculture (NIFA) (2011-51181-30767, Developing Scientifically based Consensus Food Safety Metrics for Leafy Greens and Tomatoes). This work, including the efforts of Manan Sharma and Patricia Millner, was funded by USDA vertical bar Agricultural Research Service (ARS) (Project #8042-32420-005-00, Microbial Ecology & Safety of Fresh on-Farm Organically Grown Produce). NR 25 TC 2 Z9 2 U1 5 U2 8 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JUL PY 2016 VL 82 IS 13 BP 3746 EP 3753 DI 10.1128/AEM.00052-16 PG 8 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DO8QP UT WOS:000378048800006 PM 27084022 ER PT J AU Gaskell, J Blanchette, RA Stewart, PE BonDurant, SS Adams, M Sabat, G Kersten, P Cullen, D AF Gaskell, Jill Blanchette, Robert A. Stewart, Philip E. BonDurant, Sandra Splinter Adams, Marie Sabat, Grzegorz Kersten, Phil Cullen, Dan TI Transcriptome and Secretome Analyses of the Wood Decay Fungus Wolfiporia cocos Support Alternative Mechanisms of Lignocellulose Conversion SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID BASIDIOMYCETE PHANEROCHAETE-CHRYSOSPORIUM; BROWN-ROT; POSTIA-PLACENTA; GLOEOPHYLLUM-TRABEUM; DEGRADATION; CELLULOSE; DATABASE; GENOMES; OXIDASE; IRON AB Certain wood decay basidiomycetes, collectively referred to as brown rot fungi, rapidly depolymerize cellulose while leaving behind the bulk of cell wall lignin as a modified residue. The mechanism(s) employed is unclear, but considerable evidence implicates the involvement of diffusible oxidants generated via Fenton-like chemistry. Toward a better understanding of this process, we have examined the transcriptome and secretome of Wolfiporia cocos when cultivated on media containing glucose, purified crystalline cellulose, aspen (Populus grandidentata), or lodgepole pine (Pinus contorta) as the sole carbon source. Compared to the results obtained with glucose, 30, 183, and 207 genes exhibited 4-fold increases in transcript levels in cellulose, aspen, and lodgepole pine, respectively. Mass spectrometry identified peptides corresponding to 64 glycoside hydrolase (GH) proteins, and of these, 17 corresponded to transcripts upregulated on one or both woody substrates. Most of these genes were broadly categorized as hemicellulases or chitinases. Consistent with an important role for hydroxyl radical in cellulose depolymerization, high transcript levels and upregulation were observed for genes involved in iron homeostasis, iron reduction, and extracellular peroxide generation. These patterns of regulation differ markedly from those of the closely related brown rot fungus Postia placenta and expand the number of enzymes potentially involved in the oxidative depolymerization of cellulose. C1 [Gaskell, Jill; Kersten, Phil; Cullen, Dan] USDA, Forest Prod Lab, Madison, WI 53705 USA. [Blanchette, Robert A.] Univ Minnesota, Dept Plant Pathol, St Paul, MN USA. [Stewart, Philip E.] NIAID, Rocky Mt Labs, NIH, Hamilton, MT 59840 USA. [BonDurant, Sandra Splinter; Adams, Marie; Sabat, Grzegorz] Univ Wisconsin, Madison Biotechnol Ctr, Madison, WI USA. RP Cullen, D (reprint author), USDA, Forest Prod Lab, Madison, WI 53705 USA. EM dcullen@wisc.edu FU National Research Initiative of the U.S. Department of Agriculture Cooperative State, Research Education and Extension Service grant [2007 35504 18257] FX This work was supported in part by the National Research Initiative of the U.S. Department of Agriculture Cooperative State, Research Education and Extension Service grant 2007 35504 18257, to D.C. and R.A.B. NR 47 TC 0 Z9 0 U1 7 U2 15 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JUL PY 2016 VL 82 IS 13 BP 3979 EP 3987 DI 10.1128/AEM.00639-16 PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DO8QP UT WOS:000378048800030 PM 27107121 ER PT J AU Lao, F Brown-Brandl, T Stinn, JP Liu, K Teng, G Xin, H AF Lao, F. Brown-Brandl, T. Stinn, J. P. Liu, K. Teng, G. Xin, H. TI Automatic recognition of lactating sow behaviors through depth image processing SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE Animal welfare; Depth image; Image processing; Sow behaviors ID ACCELERATION PATTERNS; THERMAL COMFORT; PIGS; CLASSIFICATION; PERFORMANCE AB Manual observation and classification of animal behaviors is laborious, time-consuming, and of limited ability to process large amount of data. A computer vision-based system was developed that automatically recognizs sow behaviors (lying, sitting, standing, kneeling, feeding, drinking, and shifting) in farrowing crate. The system consisted of a low-cost 3D camera that simultaneously acquires digital and depth images and a software program that detects and identifies the sow's behaviors. This paper describes the computational algorithm for the analysis of depth images and presents its performance in recognizing the sow's behaviors as compared to manual recognition. The images were acquired at 6 s intervals on three days of a 21-day lactation period. Based on analysis of the 6 s interval images, the algorithm had the following accuracy of behavioral classification: 99.9% in lying, 96.4% in sitting, 99.2% in standing, 78.1% in kneeling, 97.4% in feeding, 92.7% in drinking, and 63.9% in transitioning between behaviors. The lower classification accuracy for the transitioning category presumably stemmed from insufficient frequency of the image acquisition which can be readily improved. Hence the reported system provides an effective way to automatically process and classify the sow's behavioral images. This tool is conducive to investigating behavioral responses and time budget of lactating sows and their litters to farrowing crate designs and management practices. (C) 2016 Elsevier B.V. All rights reserved. C1 [Lao, F.] China Agr Univ, Network Ctr, Beijing 100094, Peoples R China. [Brown-Brandl, T.] USDA ARS, Meat Anim Res Ctr, Clay Ctr, NE USA. [Stinn, J. P.] Iowa Select Farms, Iowa Falls, IA USA. [Liu, K.; Xin, H.] Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA USA. [Teng, G.] China Agr Univ, Coll Water Resources & Civil Engn, Beijing 100094, Peoples R China. RP Xin, H (reprint author), Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA USA. EM laofd@cau.edu.cn; hxin@iastate.edu OI Liu, Kai/0000-0002-1556-1068 FU College of Agriculture and Life Sciences at Iowa State University; USDA-ARS Meat Animal Research Center; "Twelfth Five-Year-Plan" for National Science and Technology for Rural Development in China [2014BAD08B05] FX This study was funded in part by the College of Agriculture and Life Sciences at Iowa State University, USDA-ARS Meat Animal Research Center, and the "Twelfth Five-Year-Plan" for National Science and Technology for Rural Development in China (2014BAD08B05) - China. Special thanks go to the staff of the USDA Meat Animal Research Center for their assistance in data collection and animal care. NR 32 TC 0 Z9 1 U1 9 U2 21 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 EI 1872-7107 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD JUL PY 2016 VL 125 BP 56 EP 62 DI 10.1016/j.compag.2016.04.026 PG 7 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA DP0PI UT WOS:000378191300006 ER PT J AU Cleveland, BM Weber, GM AF Cleveland, B. M. Weber, G. M. TI Effects of steroid treatment on growth, nutrient partitioning, and expression of genes related to growth and nutrient metabolism in adult triploid rainbow trout (Oncorhynchus mykiss) SO DOMESTIC ANIMAL ENDOCRINOLOGY LA English DT Article DE Fish; Nutrient partitioning; Fillet yield; Maturation ID FACTOR-BINDING-PROTEINS; MESSENGER-RNA LEVELS; SKELETAL-MUSCLE; FACTOR-I; IGF-I; OREOCHROMIS-MOSSAMBICUS; SALVELINUS-FONTINALIS; TRENBOLONE ACETATE; SEXUAL-MATURATION; SALMO-GAIRDNERII AB The contribution of sex steroids to nutrient partitioning and energy balance during gonad development was studied in rainbow trout. Specifically, 19-mo old triploid (3N) female rainbow trout were fed treatment diets supplemented with estradiol-17 beta (E2), testosterone (T), or dihydrotestosterone at 30-mg steroid/kg diet for a 1-mo period. Growth performance, nutrient partitioning, and expression of genes central to growth and nutrient metabolism were compared with 3N and age-matched diploid (2N) female fish consuming a control diet not supplemented with steroids. Only 2 N fish exhibited active gonad development, with gonad weights increasing from 3.7% to 5.5% of body weight throughout the study, whereas gonad weights in 3N fish remained at 0.03%. Triploid fish consuming dihydrotestosterone exhibited faster specific growth rates than 3N-controls (P < 0.05). Consumption of E2 in 3N fish reduced fillet growth and caused lower fillet yield compared with all other treatment groups (P < 0.05). In contrast, viscera fat gain was not affected by steroid consumption (P > 0.05). Gene transcripts associated with physiological pathways were identified in maturing 2N and E2-treated 3N fish that differed in abundance from 3N control fish (P < 0.05). In liver these mechanisms included the growth hormone/insulin-like growth factor (IGF) axis (igf1, igf2), IGF binding proteins (igfbp1b1, igfbp2b1, igfbp5b1, igfbp6b1), and genes associated with lipid binding and transport (fabp3, fabp4, Ipl, cd36), fatty acid oxidation (cpt1a), and the pparg transcription factor. In muscle, these mechanisms included reductions in myogenic gene expression (fst, myog) and the proteolysis related gene, cathepsin-L, suggesting an E2-induced reduction in the capacity for muscle growth. These findings suggest that increased E2 signaling in the sexually maturing female rainbow trout alters physiological pathways in liver, particularly those related to IGF signaling and lipid metabolism, to partition nutrients away from muscle growth toward support of maturation-related processes. In contrast, the mobilization of viscera lipid stores appear to be mediated less by E2 and more by energy demands associated with gonad development. These findings improve the understanding of how steroids regulate nutrient metabolism to meet the high energy demands associated with gonad development during sexual maturation. Published by Elsevier Inc. C1 [Cleveland, B. M.; Weber, G. M.] ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, Kearneysville, WV 25430 USA. RP Cleveland, BM (reprint author), ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, Kearneysville, WV 25430 USA. EM beth.cleveland@ars.usda.gov NR 68 TC 2 Z9 2 U1 15 U2 18 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0739-7240 EI 1879-0054 J9 DOMEST ANIM ENDOCRIN JI Domest. Anim. Endocrinol. PD JUL PY 2016 VL 56 BP 1 EP 12 DI 10.1016/j.domaniend.2016.01.001 PG 12 WC Agriculture, Dairy & Animal Science; Endocrinology & Metabolism SC Agriculture; Endocrinology & Metabolism GA DP4CU UT WOS:000378443800001 PM 26905215 ER PT J AU Sanchez, NCB Carroll, JA Broadway, PR Hughes, HD Roberts, SL Richeson, JT Schmidt, TB Vann, RC AF Sanchez, N. C. Burdick Carroll, J. A. Broadway, P. R. Hughes, H. D. Roberts, S. L. Richeson, J. T. Schmidt, T. B. Vann, R. C. TI Cattle temperament influences metabolism: metabolic response to glucose tolerance and insulin sensitivity tests in beef steers SO DOMESTIC ANIMAL ENDOCRINOLOGY LA English DT Article DE Cattle; Glucose; Insulin; Insulin sensitivity; Nonesterified fatty acids; Temperament ID FATTY-ACIDS; DAIRY-COWS; NORTHERN QUEENSLAND; RECTAL TEMPERATURE; BODY-TEMPERATURE; FEEDLOT CATTLE; TECHNICAL NOTE; BRAHMAN BULLS; ASSOCIATIONS; CARCASS AB Cattle temperament, defined as the reactivity of cattle to humans or novel environments, can greatly influence several physiological systems in the body, including immunity, stress, and most recently discovered, metabolism. Greater circulating concentrations of nonesterified fatty acids (NEFAs) found in temperamental cattle suggest that temperamental cattle are metabolically different than calm cattle. Further, elevated NEFA concentrations have been reported to influence insulin sensitivity. Therefore, the objective of this study was to determine whether cattle temperament would influence the metabolic response to a glucose tolerance test (GTT) and insulin sensitivity test (IST). Angus-cross steers (16 calm and 15 temperamental; 216 +/- 6 kg BW) were selected based on temperament score measured at weaning. On day 1, steers were moved into indoor stanchions to allow measurement of individual ad libitum feed intake. On day 6, steers were fitted with indwelling rectal temperature probes and jugular catheters. At 9 AM on day 7, steers received the GTT (0.5-mL/kg BW of a 50% dextrose solution), and at 2 PM on day 7, steers received the IST (2.5 IU bovine insulin/kg BW). Blood samples were collected and serum isolated at -60, -45, -30, -15, 0, 10, 20, 30, 45, 60, 90, 120, and 150 min relative to each challenge. Serum was stored at -80 degrees C until analyzed for cortisol, glucose, NEFA, and blood urea nitrogen concentrations. All variables changed over time (P < 0.01). For the duration of the study, temperamental steers maintained greater (P < 0.01) serum NEFA and less (P < 0.01) serum blood urea nitrogen and insulin sensitivity (calculated using Revised Quantitative Insulin Sensitivity Check Index) compared with calm steers. During the GTT, temperamental steers had greater (P < 0.01) serum glucose, yet decreased (P = 0.03) serum insulin and (P < 0.01) serum insulin: serum glucose compared to calm cattle. During the IST, temperamental steers had greater (P < 0.01) serum insulin and a greater (P < 0.01) serum insulin: serum glucose as compared with calm steers. These data demonstrate that differences exist in the manner in which temperamental steers respond to glucose and insulin, potentially a result of elevated serum NEFA concentrations, which may result in changes in utilization and redistribution of energy in temperamental vs calm cattle. Published by Elsevier Inc. C1 [Sanchez, N. C. Burdick; Carroll, J. A.; Broadway, P. R.] ARS, Livestock Issues Res Unit, USDA, Lubbock, TX 79403 USA. [Hughes, H. D.; Roberts, S. L.; Richeson, J. T.] West Texas A&M Univ, Dept Agr Sci, Canyon, TX 79016 USA. [Schmidt, T. B.] Univ Nebraska, Dept Anim Sci, Lincoln, NE 68583 USA. [Vann, R. C.] Mississippi State Univ, MAFES Brown Loam, Raymond, MS 39154 USA. RP Carroll, JA (reprint author), ARS, Livestock Issues Res Unit, USDA, Lubbock, TX 79403 USA. EM jeff.carroll@ars.usda.gov OI Burdick Sanchez, Nicole/0000-0003-0709-871X NR 39 TC 0 Z9 0 U1 9 U2 11 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0739-7240 EI 1879-0054 J9 DOMEST ANIM ENDOCRIN JI Domest. Anim. Endocrinol. PD JUL PY 2016 VL 56 BP 85 EP 95 DI 10.1016/j.domaniend.2016.02.009 PG 11 WC Agriculture, Dairy & Animal Science; Endocrinology & Metabolism SC Agriculture; Endocrinology & Metabolism GA DP4CU UT WOS:000378443800011 ER PT J AU Xu, DH Zhang, QZ Shoemaker, CA Zhang, DH Moreira, GSA AF Xu, De-Hai Zhang, Qi-Zhong Shoemaker, Craig A. Zhang, Dunhua Moreira, Gabriel S. A. TI Molecular immune response of channel catfish immunized with live theronts of Ichthyophthirius multifiliis SO FISH & SHELLFISH IMMUNOLOGY LA English DT Article DE Immunization; Serum antibody; Survival; Channel catfish; Ichthyophthirius multifiliis; Immune-related genes; qPCR ID ICTALURUS-PUNCTATUS RAFINESQUE; ONCORHYNCHUS-MYKISS WALBAUM; TOLL-LIKE RECEPTORS; CYPRINUS-CARPIO L; RAINBOW-TROUT; EXPRESSION PROFILE; ANTIBODY-TITERS; RELEVANT GENES; INFECTION; FOUQUET AB The parasite Ichthyophthirius multifiliis (Ich) has been reported in various freshwater fishes worldwide and results in severe losses to both food and aquarium fish production. The fish surviving natural infections or immunized with live theronts develop strong specific and non-specific immune responses. Little is known about how these immune genes are induced or how they interact and lead to specific immunity against Ichthyophthirius multifiliis in channel catfish Ictalurus punctatus. This study evaluated the differential expression of immune-related genes, including immunoglobulin, immune cell receptor, cytokine, complement factor and toll-like receptors in head kidney from channel catfish at different time points after immunization with live theronts of I. multifiliis. The immunized fish showed significantly higher anti-Ich antibody expressed as immobilization titer and ELISA titer than those of control fish. The vast majority of immunized fish (95%) survived theront challenge. Expression of IgM and IgD heavy chain genes exhibited a rapid increase from 4 hour (h4) to 2 days (d2) post immunization. Expression of immune cell receptor genes (CD4, CD8-alpha, MHC I, MHC II beta, TcR-alpha, and TcR-beta) showed up-regulation from h4 to d6 post immunization, indicating that different immune cells were actively involved in cellular immune response. Cytokine gene expression (IL-1 beta a, IL-1 beta b, IFN-gamma and TNF-alpha) increased rapidly at h4 post immunization and were at an up-regulated level until d2 compared to the bovine serum albumin control. Expression of complement factor and toll-like receptor genes exhibited a rapid increase from h4 to d2 post immunization. Results of this study demonstrated differential expression of genes involved in the specific or non-specific immune response post immunization and that the vaccination against Ich resulted in protection against infection by I. multifiliis. Published by Elsevier Ltd. C1 [Xu, De-Hai; Shoemaker, Craig A.; Zhang, Dunhua] ARS, USDA, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 USA. [Zhang, Qi-Zhong] Jinan Univ, Inst Hydrobiol, Key Lab Eutrophicat & Red Tide Prevent, Guangdong Higher Educ Inst, Guangzhou 510632, Guangdong, Peoples R China. [Moreira, Gabriel S. A.] Univ Sao Paulo, Coll Anim Sci & Food Engn, Parasitol Lab, Av Duque Caxias Norte 225, BR-13635900 Pirassununga, SP, Brazil. RP Xu, DH (reprint author), ARS, USDA, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 USA. EM dehai.xu@ars.usda.gov FU FAPESP [2015/01822-0]; USDA/ARS CRIS Project [6010-32000-026-00D] FX The authors thank Dr. Jason Abernathy, USDA-ARS, Hagerman Fish Culture Experiment Station, Hagerman and Dr. Xing-Jiang Mu, College of Fisheries and Life Science, Shanghai Ocean University for valuable comments and suggestions to improve the manuscript. We gratefully acknowledge the technical assistance of Jana Mladek, Paige Mumma and the management team of the Aquatic Animal Health Research Unit aquatic facility for daily care and management of fish. We are also grateful to the FAPESP for providing a scholarship to Gabriel S. A. Moreira (Proc. no. 2015/01822-0). This research was supported by USDA/ARS CRIS Project #6010-32000-026-00D. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 37 TC 2 Z9 2 U1 8 U2 17 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1050-4648 EI 1095-9947 J9 FISH SHELLFISH IMMUN JI Fish Shellfish Immunol. PD JUL PY 2016 VL 54 BP 86 EP 92 DI 10.1016/j.fsi.2016.03.166 PG 7 WC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences GA DP4FM UT WOS:000378451700011 PM 27044331 ER PT J AU Yun, CH Yin, TJ Shatzer, K Burrin, DG Cui, LW Tu, YF Hu, M AF Yun, Changhong Yin, Taijun Shatzer, Katherine Burrin, Douglas G. Cui, Liwei Tu, Yifan Hu, Ming TI Determination of 7 alpha-OH cholesterol by LC-MS/MS: Application in assessing the activity of CYP7A1 in cholestatic minipigs SO JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES LA English DT Article DE LC-MS/MS; CYP7A1; Cholesterol 7 alpha-hydroxylase activity; 7 alpha-OH cholesterol; Liver microsome ID BILE-ACID SYNTHESIS; TOTAL PARENTERAL-NUTRITION; CHROMATOGRAPHY-MASS-SPECTROMETRY; 7 ALPHA-HYDROXYLASE; 7-ALPHA-HYDROXYLASE ACTIVITY; LIVER-DISEASE; NUCLEAR RECEPTORS; ASSAY; MECHANISMS; 4-BETA-HYDROXYCHOLESTEROL AB An LC-MS/MS method was developed and validated to determine 7 alpha-OH cholesterol in liver microsome. This method was convenient and fast with high specificity and sensitivity. Briefly, a gradient elution was performed on a Synergi polar-C18 column (50 x 4.6 mm i.d., 3 mu m). The mobile phase (consisting of 0.1% HCOOH solution and acetonitrile) eluted in gradient at a flow rate of 1 ml/min. MS detection was operated on APCI (+) mode; the MRM transitions for 7 alpha-OH cholesterol and D7-cholesterol (I.S.) were 385.1 >= 159.1 and 376.4 >= 266.3, respectively. The linear response range of 7 alpha-OH cholesterol was covered from 1.563 to 100.0 ng/ml. All of the validation items meet the requirement of FDA guidance for bioanalytical method validation. This method was applied to enzymatic studies for determination of cholesterol 7alpha-hydroxylation activity catalyzed by CYP7A1 in the cholestatic minipigs liver microsomes. (C) 2016 Elsevier B.V. All rights reserved. C1 [Yun, Changhong; Yin, Taijun; Shatzer, Katherine; Tu, Yifan; Hu, Ming] Univ Houston, Dept Pharmaceut, 1441 Moursund St, Houston, TX 77030 USA. [Burrin, Douglas G.; Cui, Liwei] Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, USDA, 1100 Bates St, Houston, TX 77030 USA. RP Hu, M (reprint author), Univ Houston, Dept Pharmaceut, 1441 Moursund St, Houston, TX 77030 USA. EM mhu@uh.edu FU NIGMS NIH HHS [R01 GM070737] NR 46 TC 0 Z9 0 U1 4 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1570-0232 EI 1873-376X J9 J CHROMATOGR B JI J. Chromatogr. B PD JUL 1 PY 2016 VL 1025 BP 76 EP 82 DI 10.1016/j.jchromb.2016.05.005 PG 7 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA DP3AP UT WOS:000378365100010 PM 27218859 ER PT J AU Chastain, DR Snider, JL Choinski, JS Collins, GD Perry, CD Whitaker, J Grey, TL Sorensen, RB van Iersel, M Byrd, SA Porter, W AF Chastain, Daryl R. Snider, John L. Choinski, John S. Collins, Guy D. Perry, Calvin D. Whitaker, Jared Grey, Timothy L. Sorensen, Ronald B. van Iersel, Marc Byrd, Seth A. Porter, Wesley TI Leaf ontogeny strongly influences photosynthetic tolerance to drought and high temperature in Gossypium hirsutum SO JOURNAL OF PLANT PHYSIOLOGY LA English DT Article DE Gossypium hirsutum; Drought; Heat tolerance; Leaf expansion; Photosynthesisa ID PHOTOSYSTEM-II ACTIVITY; ELECTRON-TRANSPORT; WATER-STRESS; HEAT-STRESS; CLIMATE-CHANGE; MATURE LEAVES; COTTON; FIELD; THERMOSTABILITY; ACTIVATION AB Temperature and drought are major abiotic limitations to crop productivity worldwide. While abiotic stress physiology research has focused primarily on fully expanded leaves, no studies have investigated photosynthetic tolerance to concurrent drought and high temperature during leaf ontogeny. To address this, Gossypium hirsutum plants were exposed to five irrigation treatments, and two different leaf stages were sampled on three dates during an abnormally dry summer. Early in the growing season, ontogenic PSII heat tolerance differences were observed. Photosystem II was more thermotolerant in young leaves than mature leaves. Later in the growing season, no decline in young leaf net photosynthesis (P-N) was observed as leaf temperature increased from 31 to 37 degrees C, as average midday leaf water potential (psi(MD)) declined from -1.25 to -2.03 MPa. In contrast, mature leaf PN declined 66% under the same conditions. Stomatal conductance (g(s)) accounted for 84-98% of variability in leaf temperature, and gs was strongly associated with klimp in mature leaves but not in young leaves. We conclude that young leaves are more photosynthetically tolerant to heat and drought than mature leaves. Elucidating the mechanisms causing these ontogenic differences will likely help mitigate the negative impacts of abiotic stress in the future. (C) 2016 Elsevier GmbH. All rights reserved. C1 [Chastain, Daryl R.] Mississippi State Univ, Plant & Soil Sci, Delta Res & Extens Ctr, POB 197, Stoneville, MS 38776 USA. [Snider, John L.; Grey, Timothy L.; Porter, Wesley] Univ Georgia, Dept Crop & Soil Sci, 115 Coastal Way, Tifton, GA 31794 USA. [Choinski, John S.] Univ Cent Arkansas, Dept Biol, 201 Donaghey, Conway, AR 72035 USA. [Collins, Guy D.] N Carolina State Univ, Upper Coastal Plains Res Stn, Dept Crop Sci, 2811 Nobles Mill Pond Rd, Rocky Mount, NC 27801 USA. [Perry, Calvin D.] Univ Georgia, Coll Agr & Environm Sci, 8207 Georgia 37, Camilla, GA 31730 USA. [Whitaker, Jared] Univ Georgia, Dept Crop & Soil Sci, POB 8112, Gsu Statesboro, GA 30460 USA. [Sorensen, Ronald B.] ARS, USDA, Natl Peanut Res Lab, POB 509, Dawson, GA 39842 USA. [van Iersel, Marc] Univ Georgia, Dept Hort, Athens, GA 30602 USA. [Byrd, Seth A.] Texas A&M AgriLife Extens, Lubbock, TX 79403 USA. RP Chastain, DR (reprint author), Mississippi State Univ, Plant & Soil Sci, Delta Res & Extens Ctr, POB 197, Stoneville, MS 38776 USA. EM daryl.chastain@msstate.edu NR 37 TC 1 Z9 1 U1 11 U2 22 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 0176-1617 EI 1618-1328 J9 J PLANT PHYSIOL JI J. Plant Physiol. PD JUL PY 2016 VL 199 BP 18 EP 28 DI 10.1016/j.jplph.2016.05.003 PG 11 WC Plant Sciences SC Plant Sciences GA DO9DR UT WOS:000378085000003 ER PT J AU Herring, D Chang, S Bard, S Gavey, E AF Herring, David Chang, Sarah Bard, Sasha Gavey, Erica TI Five Years of MyPlate-Looking Back and What's Ahead SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Editorial Material C1 [Herring, David; Chang, Sarah; Bard, Sasha] USDA, Ctr Nutr Policy & Promot, Alexandria, VA USA. [Gavey, Erica] Panum Grp, Bethesda, MD USA. RP Herring, D (reprint author), USDA, Ctr Nutr Policy & Promot, Alexandria, VA USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD JUL PY 2016 VL 116 IS 7 BP 1069 EP 1071 DI 10.1016/j.jand.2016.05.001 PG 3 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DP2OW UT WOS:000378329600003 PM 27343970 ER PT J AU Hansen, CP Schreiber, LA Rumble, MA Millspaugh, JJ Gamo, RS Kehmeier, JW Wojcik, N AF Hansen, Christopher P. Schreiber, Leslie A. Rumble, Mark A. Millspaugh, Joshua J. Gamo, R. Scott Kehmeier, Jon W. Wojcik, Nate TI Microsite Selection and Survival of Greater Sage-Grouse Nests in South-Central Wyoming SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE Centrocercus urophasianus; GPS PTT; greater sage-grouse; microsite; nest; selection; survival; Wyoming ID ENERGY DEVELOPMENT; CENTROCERCUS-UROPHASIANUS; HABITAT SELECTION; VISUAL OBSTRUCTION; RESOURCE SELECTION; SITE SELECTION; EASTERN EDGE; MONO COUNTY; POPULATIONS; CALIFORNIA AB Greater sage-grouse (Centrocercus urophasianus; hereafter, sage-grouse) populations have declined across North America because of degradation of sagebrush (Artemisia spp.) ecosystems; thus, management for sage-grouse habitat is a high priority. Expansion of some energy development has put additional pressures on sage-grouse populations and their habitats. However, little is known about the effects wind energy development may have on sage-grouse populations. Population dynamics of sage-grouse are partially related to nest success, which may be influenced by the quality of nesting habitat. Consequently, knowledge of vegetation and structural characteristics associated with selection and survival of sage-grouse nests would help inform management decisions to improve nest success. As part of a study quantifying greater sage-grouse demographics prior to construction of a wind energy facility, we monitored female sage-grouse equipped with 30-g solar Argos global positioning system platform transmitter terminals during the breeding season in Carbon County, Wyoming to assess selection and survival of nests at nest area (30-m radius), nest patch (5-m radius), and nest bowl scales. Sage-grouse selected nest sites with increased visual obstruction from 22.9-45.7 cm in height at all scales, less forb cover at the nest area, and more sagebrush cover at the nest patch. Survival of nests to 27 days (i.e., incubation period) was 43.3% (95% CI: 31.8-59.1%) in 2011 (n = 49), 33.2% (95% CI: 21.5-51.3%) in 2012 (n = 40), and 25.1% (95% CI: 15.0-42.0%) in 2013 (n = 39). Grass height had a positive association with survival of nests at the nest area and nest patch, canopy cover of bunchgrasses and sagebrush had an interactive effect on survival of nests at the nest patch, and visual obstruction from 0-22.9 cm in height had a positive relationship with survival of nests at the nest bowl. The standardized precipitation-evapotranspiration index (SPEI), an index of drought, from the previous growing season was also negatively correlated with survival of nests. Our results suggest management for nesting sage-grouse should focus on a heterogeneous compilation of tall bunchgrasses > 15 cm and patches of sagebrush cover > 30%. Management for grasses > 15 cm tall should be especially diligent during drought years, given SPEI was associated with nest survival the following year. Although there could be other factors important to selection or survival of nests that we did not evaluate, our results suggest scale-dependent management strategies at the microsite level may not be necessary. We encourage researchers and managers to use our baseline results in a before-after control-impact design with future, post-construction data to determine the potential effects of wind energy development on selection and survival of sage-grouse nests. (C) 2016 The Wildlife Society. C1 [Hansen, Christopher P.; Schreiber, Leslie A.; Millspaugh, Joshua J.] Univ Missouri, Dept Fisheries & Wildlife Sci, 302 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. [Rumble, Mark A.] US Forest Serv, Rocky Mt Res Stn, 8221 South Highway 16, Rapid City, SD 57702 USA. [Gamo, R. Scott] Wyoming Game & Fish Dept, 5400 Bishop Ave, Cheyenne, WY 82006 USA. [Kehmeier, Jon W.; Wojcik, Nate] SWCA Environm Consultants, 295 Interlocken Blvd Suite 300, Broomfied, CO 80021 USA. [Rumble, Mark A.] 1104 North 16th, Silt, CO 81652 USA. RP Millspaugh, JJ (reprint author), Univ Missouri, Dept Fisheries & Wildlife Sci, 302 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. EM millspaughj@missouri.edu FU U.S. Forest Service Rocky Mountain Research Station [10-JV-11221632-215]; Wyoming Game and Fish Department [11-CO-112216-018]; Western Association of Fish and Wildlife Agencies [11-CO-11221632-068, 12-CO-11221632-181]; National Renewable Energy Laboratory; National Fish and Wildlife Foundation; Bureau of Land Management; Power Company of Wyoming LLC [10-CO-11221632-238]; SWCA Environmental Consultants; National Wind Coordinating Collaborative; American Wind Wildlife Institute FX A. L. Fremgen and many field technicians assisted with sage-grouse tagging, tracking, and associated field work, and L. S. Baggett assisted with development and interpretation of statistical analyses. Two anonymous reviewers provided helpful comments that improved the manuscript. The research associated with this publication resulted from research funded, in part, by U.S. Forest Service Rocky Mountain Research Station (10-JV-11221632-215), Wyoming Game and Fish Department (11-CO-112216-018), Western Association of Fish and Wildlife Agencies (11-CO-11221632-068 and 12-CO-11221632-181), National Renewable Energy Laboratory, National Fish and Wildlife Foundation, Bureau of Land Management, Power Company of Wyoming LLC (10-CO-11221632-238), and SWCA Environmental Consultants. The National Wind Coordinating Collaborative and American Wind Wildlife Institute helped to acquire funds for our research program focused on sage-grouse ecology and wind energy development. NR 81 TC 0 Z9 0 U1 18 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD JUL PY 2016 VL 80 IS 5 BP 862 EP 876 DI 10.1002/jwmg.1065 PG 15 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA DP2PS UT WOS:000378332000009 ER PT J AU Trehan, I Manary, MJ AF Trehan, Indi Manary, Mark J. TI An important chapter in the infection-malnutrition story SO LANCET GLOBAL HEALTH LA English DT Editorial Material ID CHILDREN; COTRIMOXAZOLE; TRIAL C1 [Trehan, Indi; Manary, Mark J.] Washington Univ, Dept Pediat, St Louis, MO 63110 USA. [Trehan, Indi] Univ Malawi, Dept Paediat & Child Hlth, Blantyre, Malawi. [Manary, Mark J.] Univ Malawi, Dept Community Hlth, Blantyre, Malawi. [Manary, Mark J.] USDA, Childrens Nutr Res Ctr, Baylor Coll Med, Houston, TX USA. RP Manary, MJ (reprint author), Washington Univ, Dept Pediat, St Louis, MO 63110 USA.; Manary, MJ (reprint author), Univ Malawi, Dept Community Hlth, Blantyre, Malawi.; Manary, MJ (reprint author), USDA, Childrens Nutr Res Ctr, Baylor Coll Med, Houston, TX USA. EM manary@kids.wustl.edu NR 12 TC 0 Z9 0 U1 4 U2 4 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 2214-109X J9 LANCET GLOB HEALTH JI Lancet Glob. Health PD JUL PY 2016 VL 4 IS 7 BP E430 EP E431 DI 10.1016/S2214-109X(16)30110-3 PG 2 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA DP0IG UT WOS:000378172700003 PM 27289200 ER PT J AU Walker, BJ South, PF Ort, DR AF Walker, Berkley J. South, Paul F. Ort, Donald R. TI Physiological evidence for plasticity in glycolate/glycerate transport during photorespiration SO PHOTOSYNTHESIS RESEARCH LA English DT Article DE Photorespiration; Photosynthesis; Quantum efficiency; Abiotic stress; Central metabolism; Photosynthetic models ID CARBOXYLASE OXYGENASE ACTIVATION; GLYCOLATE GLYCERATE TRANSPORTER; TRANSGENIC TOBACCO; CHLOROPHYLL FLUORESCENCE; GAS-EXCHANGE; GLYOXYLATE INHIBITION; ARABIDOPSIS-THALIANA; RUBISCO ACTIVASE; REDUCED AMOUNTS; ABSENCE CAUSES AB Photorespiration recycles fixed carbon following the oxygenation reaction of Ribulose, 1-5, carboxylase oxygenase (Rubisco). The recycling of photorespiratory C2 to C3 intermediates is not perfectly efficient and reduces photosynthesis in C3 plants. Recently, a plastidic glycolate/glycerate transporter (PLGG1) in photorespiration was identified in Arabidopsis thaliana, but it is not known how critical this transporter is for maintaining photorespiratory efficiency. We examined a mutant deficient in PLGG1 (plgg1-1) using modeling, gas exchange, and Rubisco biochemistry. Under low light (under 65 mu mol m(-2) s(-1) PAR), there was no difference in the quantum efficiency of CO2 assimilation or in the photorespiratory CO2 compensation point of plgg1-1, indicating that photorespiration proceeded with wild-type efficiency under sub-saturating light irradiances. Under saturating light irradiance (1200 mu mol m(-2) s(-1) PAR), plgg1-1 showed decreased CO2 assimilation that was explained by decreases in the maximum rate of Rubisco carboxylation and photosynthetic linear electron transport. Decreased rates of Rubisco carboxylation resulted from probable decreases in the Rubisco activation state. These results suggest that glycolate/glycerate transport during photorespiration can proceed in moderate rates through an alternative transport process with wild-type efficiencies. These findings also suggest that decreases in net CO2 assimilation that occur due to disruption to photorespiration can occur by decreases in Rubisco activity and not necessarily decreases in the recycling efficiency of photorespiration. C1 [Walker, Berkley J.; South, Paul F.; Ort, Donald R.] ARS, Global Change & Photosynth Res Unit, USDA, Urbana, IL 61801 USA. [Walker, Berkley J.; South, Paul F.; Ort, Donald R.] Univ Illinois, Carl R Woese Inst Genom Biol, Urbana, IL 61801 USA. [Ort, Donald R.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Walker, Berkley J.] Univ Dusseldorf, Cluster Excellence Plant Sci, Inst Plant Biochem, D-40225 Dusseldorf, Germany. RP Walker, BJ (reprint author), ARS, Global Change & Photosynth Res Unit, USDA, Urbana, IL 61801 USA.; Walker, BJ (reprint author), Univ Illinois, Carl R Woese Inst Genom Biol, Urbana, IL 61801 USA.; Walker, BJ (reprint author), Univ Dusseldorf, Cluster Excellence Plant Sci, Inst Plant Biochem, D-40225 Dusseldorf, Germany. EM walkerb@uni-duesseldorf.de; pfsouth@illinois.edu; d-ort@illinois.edu FU Bill and Melinda Gates Foundation [OPP1060461] FX This research was supported via subcontract by the Bill and Melinda Gates Foundation (OPP1060461) titled 'RIPE-Realizing Increased Photosynthetic Efficiency for Sustainable Increases in Crop Yield.' We thank the SALK institute Genomic Analysis Laboratory for providing the sequence-indexed Arabidopsis t-DNA insertional mutant plgg1-1 and Jessica Ayers, Beau Barber and Elliot Brazil for experimental support. Rubisco activity assays were performed by Cody Jones. NR 45 TC 0 Z9 0 U1 8 U2 18 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0166-8595 EI 1573-5079 J9 PHOTOSYNTH RES JI Photosynth. Res. PD JUL PY 2016 VL 129 IS 1 BP 93 EP 103 DI 10.1007/s11120-016-0277-3 PG 11 WC Plant Sciences SC Plant Sciences GA DP3QX UT WOS:000378411100009 PM 27251551 ER PT J AU Bernal, B McKinley, DC Hungate, BA White, PM Mozdzer, TJ Megonigal, JP AF Bernal, Blanca McKinley, Duncan C. Hungate, Bruce A. White, Paul M. Mozdzer, Thomas J. Megonigal, J. Patrick TI Limits to soil carbon stability; Deep, ancient soil carbon decomposition stimulated by new labile organic inputs SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Alanine; Glucose; Deep carbon; Priming; Global change ID SCRUB-OAK ECOSYSTEM; ELEVATED CO2; MICROBIAL COMMUNITY; AMINO-ACIDS; LITTER DECOMPOSITION; DIOXIDE ENRICHMENT; ENZYMATIC-ACTIVITY; ATMOSPHERIC CO2; GRASSLAND SOIL; CLIMATE-CHANGE AB Carbon (C) buried deep in soil (below 1 m) is often hundreds to thousands of years old, though the stability and sensitivity of this deep C to environmental change are not well understood. We examined the C dynamics in three soil horizons and their responses to changes in substrate availability in a coarse textured sandy spodosol (0.0-0.1, 1.0-13, and 2.7-3.0 m deep). Substrate additions were intended to mimic an increase in root exudates and available inorganic nitrogen (N) that would follow an increase of belowground biomass at depth, as previously found in a long-term CO2 enrichment experiment at this site. We incubated these soils for 60 days with glucose, alanine, and leaf litter, crossed with an inorganic N amendment equivalent to three times ambient concentrations. The organic substrates were isotopically labeled (C-13), allowing us to determine the source of mineralized C and assess the priming effect. Enzyme activity increased as much as 13 times in the two deeper horizons (1.0-1.3, and 2.7-3.0 m) after the addition of the organic substrates, even though the deepest horizon had microbial biomass and microbial phospholipid fatty acids below the level of detection before the experiment. The deepest horizon (2.7-3.0 m) yielded the largest priming response under alanine, indicating that microorganisms in these soil horizons can become active in response to input of organic substrates. Inorganic N amendments significantly decreased the priming effect, suggesting that decomposition may not be N limited. However, alanine (organic N) yielded the highest priming effect at every soil depth, indicating the importance of differentiating effect of organic and inorganic N on decomposition. Distinct priming effects with depth suggest that portions of the soil profile can respond differently to organic inputs. Our findings indicate that the deep soil C pools might be more vulnerable to environmental or anthropogenic change than previously thought, potentially influencing net CO2 exchange estimates between the land and the atmosphere. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Bernal, Blanca; McKinley, Duncan C.; Megonigal, J. Patrick] Smithsonian Environm Res Ctr, POB 28,647 Contees Wharf Rd, Edgewater, MD 21037 USA. [Hungate, Bruce A.] No Arizona Univ, Dept Biol Sci, POB 5640,617 S Beaver St, Flagstaff, AZ 86011 USA. [White, Paul M.] ARS, USDA, Sugarcane Res Unit, 5883 USDA Rd, Houma, LA 70360 USA. [Mozdzer, Thomas J.] Bryn Mawr Coll, Dept Biol, 101 N Merion Ave, Bryn Mawr, PA 19010 USA. RP Bernal, B; Megonigal, JP (reprint author), Smithsonian Environm Res Ctr, POB 28,647 Contees Wharf Rd, Edgewater, MD 21037 USA. EM bernalb@si.edu; dcmckinley@fs.fed.us; bruce.hungate@nau.edu; paul.white@ars.usda.gov; tmozdzer@brynmawr.edu; megonigalp@si.edu OI Mozdzer, Thomas/0000-0002-1053-0967 FU National Science Foundation [DEB-0445324]; Department of Energy TES program [DE-SC0008339]; NASA Kennedy Space Center; US Fish and Wildlife Service, Merritt Island National Wildlife Refuge FX This work was supported by a grant from the National Science Foundation (DEB-0445324) and from the Department of Energy TES program (DE-SC0008339). This project was accomplished with assistance of Maria Ceron-Castilla and Dr. Jason Keller. We acknowledge the help of Dr. Sean McMahon in the data analysis, and the support of NASA Kennedy Space Center, Dynamac 457 Corp. and US Fish and Wildlife Service, Merritt Island National Wildlife Refuge. NR 82 TC 1 Z9 1 U1 36 U2 66 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD JUL PY 2016 VL 98 BP 85 EP 94 DI 10.1016/j.soilbio.2016.04.007 PG 10 WC Soil Science SC Agriculture GA DO8CC UT WOS:000378008900010 ER PT J AU Hall, SJ Silver, WL Timokhin, VI Hammel, KE AF Hall, Steven J. Silver, Whendee L. Timokhin, Vitally. I. Hammel, Kenneth E. TI Iron addition to soil specifically stabilized lignin SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Carbon stabilization; Iron; Lignin; Recalcitrance; Redox; Soil organic matter ID ORGANIC-MATTER FRACTIONS; DECOMPOSITION; TURNOVER; CARBON; DEGRADATION; GOETHITE; DYNAMICS; FIELD; NMR AB The importance of lignin as a recalcitrant constituent of soil organic matter (SOM) remains contested. Associations with iron (Fe) oxides have been proposed to specifically protect lignin from decomposition, but impacts of Fe-lignin interactions on mineralization rates remain unclear. Oxygen (O-2) fluctuations characteristic of humid tropical soils drive reductive Fe dissolution and precipitation, facilitating multiple types of Fe-lignin interactions that could variably decompose or protect lignin. We tested impacts of Fe addition on C-13 methoxyl-labeled lignin mineralization in soils that were exposed to static or fluctuating O-2. Iron addition suppressed lignin mineralization to 21% of controls, regardless of O-2 availability. However, Fe addition had no effect on soil CO2 production, implying that Fe oxides specifically protected lignin methoxyls but not bulk SOM. Iron oxide-lignin interactions represent a specific mechanism for lignin stabilization, linking SOM biochemical composition to turnover via geochemistry. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Hall, Steven J.] Iowa State Univ, Dept Ecol Evolut & Organismal Biol, Ames, IA 50011 USA. [Silver, Whendee L.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Timokhin, Vitally. I.] Univ Wisconsin, Wisconsin Energy Inst, Dept Biochem, Madison, WI 53706 USA. [Hammel, Kenneth E.] US Forest Serv, Madison, WI 53726 USA. [Hammel, Kenneth E.] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. RP Hall, SJ (reprint author), Iowa State Univ, Dept Ecol Evolut & Organismal Biol, Ames, IA 50011 USA. EM stevenjh@lastate.edu RI Hammel, Kenneth/G-1890-2011 OI Hammel, Kenneth/0000-0002-2935-5847 FU NSF [DEB-1457805]; DOE Biological and Environmental Research [DE-SC0006929]; NSF Luquillo Critical Zone Observatory [EAR-0722476]; NSF LTER [DEB-0620910] FX We thank H. Dang, C. Torrens, and the US Forest Service International Institute of Tropical Forestry. NSF grant DEB-1457805 to WLS and SJH, DOE Biological and Environmental Research grant DE-SC0006929 to KEH, and the NSF Luquillo Critical Zone Observatory (EAR-0722476) and LTER (DEB-0620910) supported this work. NR 32 TC 1 Z9 1 U1 22 U2 27 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD JUL PY 2016 VL 98 BP 95 EP 98 DI 10.1016/j.soilbio.2016.04.010 PG 4 WC Soil Science SC Agriculture GA DO8CC UT WOS:000378008900011 ER PT J AU Cheng, HL Lu, CR Yu, JZ Zou, CS Zhang, YP Wang, QL Huang, J Feng, XX Jiang, PF Yang, WC Song, GL AF Cheng, Hailiang Lu, Cairui Yu, John Z. Zou, Changsong Zhang, Youping Wang, Qiaolian Huang, Juan Feng, Xiaoxu Jiang, Pengfei Yang, Wencui Song, Guoli TI Fine mapping and candidate gene analysis of the dominant glandless gene Gl(2)(e) in cotton (Gossypium spp.) SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID BHLH TRANSCRIPTION FACTORS; UPLAND COTTON; PIGMENT GLANDS; JASMONATE RESPONSES; GENOME SEQUENCE; HIRSUTUM-L; ARABIDOPSIS; EXPRESSION; SYNTHASE; ARBOREUM AB Key message Dominant glandless gene Gl(2)(e) was fine-mapped to a 15 kb region containing one candidate gene encoding an MYC transcription factor, sequence and expression level of the gene were analyzed. Abstract Cottonseed product is an excellent source of oil and protein. However, this nutrition source is greatly limited in utilization by the toxic gossypol in pigment glands. It is reported that the Gl(2)(e) gene could effectively inhibit the formation of the pigment glands. Here, three F-2 populations were constructed using two pairs of near isogenic lines (NILs), which differ nearly only by the gland trait, for fine mapping of Gl(2)(e). DNA markers were identified from recently developed cotton genome sequence. The Gl(2)(e) gene was located within a 15-kb genomic interval between two markers CS2 and CS4 on chromosome 12. Only one gene was identified in the genomic interval as the candidate for Gl(2)(e) which encodes a family member of MYC transcription factor with 475-amino acids. Unexpectedly, the results of expression analysis indicated that the MYC gene expresses in glanded lines while almost does not express in glandless lines. These results suggest that the MYC gene probably serves as a vital positive regulator in the organogenesis pathway of pigment gland, and low expression of this gene will not launch the downstream pathway of pigment gland formation. This is the first pigment gland-related gene identification in cotton and will facilitate the research on glandless trait, cotton MYC proteins and low-gossypol cotton breeding. C1 [Cheng, Hailiang; Lu, Cairui; Zou, Changsong; Zhang, Youping; Wang, Qiaolian; Huang, Juan; Feng, Xiaoxu; Jiang, Pengfei; Yang, Wencui; Song, Guoli] Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang 455000, Henan, Peoples R China. [Yu, John Z.] ARS, USDA, Southern Plains Agr Res Ctr, Crop Germplasm Res Unit, 2881 F&B Rd, College Stn, TX 77845 USA. RP Song, GL (reprint author), Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang 455000, Henan, Peoples R China. EM sglzms@163.com FU National Hi-Tech R&D Program of China [2013AA102601]; National Natural Science Foundation of China [31271768] FX The work was supported by grants from the National Hi-Tech R&D Program of China (No. 2013AA102601) and the National Natural Science Foundation of China (No. 31271768). NR 43 TC 0 Z9 0 U1 4 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JUL PY 2016 VL 129 IS 7 BP 1347 EP 1355 DI 10.1007/s00122-016-2707-1 PG 9 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DP7LX UT WOS:000378681800008 PM 27053187 ER PT J AU Rahmatov, M Rouse, MN Nirmala, J Danilova, T Friebe, B Steffenson, BJ Johansson, E AF Rahmatov, Mahbubjon Rouse, Matthew N. Nirmala, Jayaveeramuthu Danilova, Tatiana Friebe, Bernd Steffenson, Brian J. Johansson, Eva TI A new 2DS center dot 2RL Robertsonian translocation transfers stem rust resistance gene Sr59 into wheat SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID F-SP TRITICI; SECALE-CEREALE L.; IN-SITU HYBRIDIZATION; RYE GENOME; DISEASE RESISTANCE; CHROMOSOMAL REARRANGEMENTS; CONFERRING RESISTANCE; HEXAPLOID WHEAT; PCR MARKERS; BREAD WHEAT AB Key message A new stem rust resistance gene Sr59 from Secale cereale was introgressed into wheat as a 2DS center dot 2RL Robertsonian translocation. Abstract Emerging new races of the wheat stem rust pathogen (Puccinia graminis f. sp. tritici), from Africa threaten global wheat (Triticum aestivum L.) production. To broaden the resistance spectrum of wheat to these widely virulent African races, additional resistance genes must be identified from all possible gene pools. From the screening of a collection of wheat-rye (Secale cereale L.) chromosome substitution lines developed at the Swedish University of Agricultural Sciences, we described the line 'SLU238' 2R (2D) as possessing resistance to many races of P. graminis f. sp. tritici, including the widely virulent race TTKSK (isolate synonym Ug99) from Africa. The breakage-fusion mechanism of univalent chromosomes was used to produce a new Robertsonian translocation: T2DS.2RL. Molecular marker analysis and stem rust seedling assays at multiple generations confirmed that the stem rust resistance from 'SLU238' is present on the rye chromosome arm 2RL. Line TA5094 (#101) was derived from 'SLU238' and was found to be homozygous for the T2DS.2RL translocation. The stem rust resistance gene on chromosome 2RL arm was designated as Sr59. Although introgressions of rye chromosome arms into wheat have most often been facilitated by irradiation, this study highlights the utility of the breakage-fusion mechanism for rye chromatin introgression. Sr59 provides an additional asset for wheat improvement to mitigate yield losses caused by stem rust. C1 [Rahmatov, Mahbubjon; Johansson, Eva] Swedish Univ Agr Sci, Dept Plant Breeding, POB 101, S-23053 Alnarp, Sweden. [Rouse, Matthew N.; Nirmala, Jayaveeramuthu] ARS, USDA, Cereal Dis Lab, St Paul, MN 55108 USA. [Rahmatov, Mahbubjon; Rouse, Matthew N.; Steffenson, Brian J.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Danilova, Tatiana; Friebe, Bernd] Kansas State Univ, Dept Plant Pathol, Wheat Genet Resources Ctr, Throckmorton Plant Sci Ctr, Throckmorton Hall, Manhattan, KS 66506 USA. [Rahmatov, Mahbubjon] Tajik Agr Univ, 146 Rudaki Ave, Dushanbe 734017, Tajikistan. RP Rahmatov, M (reprint author), Swedish Univ Agr Sci, Dept Plant Breeding, POB 101, S-23053 Alnarp, Sweden.; Rahmatov, M (reprint author), Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA.; Rahmatov, M (reprint author), Tajik Agr Univ, 146 Rudaki Ave, Dushanbe 734017, Tajikistan. EM mahbubjon@gmail.com RI Rouse, Matthew/G-5474-2011 FU Monsanto's Beachell-Borlaug International Fellowship; Swedish University of Agricultural Sciences; Lieberman-Okinow Endowment at the University of Minnesota; Durable Rust Resistance in Wheat Project; Bill and Melinda Gates Foundation; UK Department for International Development; USDA-ARS [5062-21220-021-00]; USDA-ARS National Plant Disease Recovery System FX Mahbubjon Rahmatov was supported through Monsanto's Beachell-Borlaug International Fellowship and the Swedish University of Agricultural Sciences. We also acknowledge support from the Lieberman-Okinow Endowment at the University of Minnesota (Brian Steffenson), the Durable Rust Resistance in Wheat Project administrated through Cornell University and supported by the Bill and Melinda Gates Foundation and UK Department for International Development (Matthew Rouse and Brian Steffenson); USDA-ARS Appropriated Project 5062-21220-021-00 (Matthew Rouse), and USDA-ARS National Plant Disease Recovery System (Matthew Rouse). We thank Sam Stoxen and Matthew Martin for their technical assistance, and Dr. Viktor Korzun and Dr. Marion Roder for providing the Xrems and Xgwm rye markers. Mention of trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the USDA, and does not imply its approval to the exclusion of other products and vendors that might also be suitable. NR 66 TC 0 Z9 0 U1 5 U2 9 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JUL PY 2016 VL 129 IS 7 BP 1383 EP 1392 DI 10.1007/s00122-016-2710-6 PG 10 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DP7LX UT WOS:000378681800011 PM 27025509 ER PT J AU Basche, AD Kaspar, TC Archontoulis, SV Jaynes, DB Sauer, TJ Parkin, TB Miguez, FE AF Basche, Andrea D. Kaspar, Thomas C. Archontoulis, Sotirios V. Jaynes, Dan B. Sauer, Thomas J. Parkin, Timothy B. Miguez, Fernando E. TI Soil water improvements with the long-term use of a winter rye cover crop SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Midwest Corn Belt; Cover crop; Soil water storage; Plant available water; Water content at field capacity; Plant growth ID CENTRAL UNITED-STATES; CLIMATE-CHANGE; PHYSICAL-PROPERTIES; ORGANIC-MATTER; TILE DRAINAGE; YIELD; SYSTEMS; IMPACTS; CARBON; FIELD AB The Midwestern United States, a region that produces one-third of maize and one-quarter of soybean grain globally, is projected to experience increasing rainfall variability. One approach to mitigate climate impacts is to utilize crop and soil management practices that enhance soil water storage and reduce the risks of flooding as well as drought-induced crop water stress. While some research indicates that a winter cover crop in maize-soybean rotations increases soil water availability, producers continue to be concerned that water use by cover crops will reduce water for a following cash crop. We analyzed continuous in-field soil water measurements from 2008 to 2014 at a Central Iowa research site that has included a winter rye cover crop in a maize-soybean rotation for thirteen years. This period of study included years in the top third of the wettest on record (2008, 2010, 2014) as well as drier years in the bottom third (2012, 2013). We found the cover crop treatment to have significantly higher soil water storage at the 0-30 cm depth from 2012 to 2014 when compared to the no cover crop treatment and in most years greater soil water content on individual days analyzed during the cash crop growing season. We further found that the cover crop significantly increased the field capacity water content by 10-11% and plant available water by 21-22%. Finally, in 2013 and 2014, we measured maize and soybean biomass every 2-3 weeks and did not see treatment differences in crop growth, leaf area or nitrogen uptake. Final crop yields were not statistically different between the cover and no cover crop treatment in any of the seven years of this analysis. This research indicates that the long-term use of a winter rye cover crop can improve soil water dynamics without sacrificing cash crop growth in maize-soybean crop rotations in the Midwestern United States. (C) 2016 The Authors. Published by Elsevier B.V. C1 [Basche, Andrea D.; Archontoulis, Sotirios V.; Miguez, Fernando E.] Iowa State Univ, Dept Agron, Agron Hall, Ames, IA 50011 USA. [Kaspar, Thomas C.; Jaynes, Dan B.; Sauer, Thomas J.; Parkin, Timothy B.] USDA ARS, Natl Lab Agr & Environm, 2150 Pommel Dr, Ames, IA 50011 USA. [Basche, Andrea D.] Union Concerned Scientists Food & Environm Progra, 1825 K St NW,Suite 800, Washington, DC 20006 USA. RP Miguez, FE (reprint author), Iowa State Univ, Dept Agron, Agron Hall, Ames, IA 50011 USA. EM abasche@ucsusa.org; tom.kaspar@ars.usda.gov; sarchont@iastate.edu; dan.jaynes@ars.usda.gov; tom.sauer@ars.usda.gov; tim.parkin@ars.usda.gov; femiguez@iastate.edu FU USDA-NIFA [2011-68002-30190]; Leopold Center for Sustainable Agriculture [E2013-19]; Iowa State Agronomy Department Research Training Fellowship; National Science Foundation [DGE0947929] FX This research is part of a regional collaborative project supported by the USDA-NIFA, Award No. 2011-68002-30190, "Cropping Systems Coordinated Agricultural Project: Climate Change, Mitigation, and Adaptation in Corn-based Cropping Systems." Project Web site: sustainablecorn.org. This research was also financially supported by the Leopold Center for Sustainable Agriculture award number E2013-19, the Iowa State Agronomy Department Research Training Fellowship and the National Science Foundation funded GK-12 program award number DGE0947929. The authors would like to thank Michael Castellano, Lisa Schulte Moore and Andrew Lenssen for reviewing drafts of this manuscript. The authors would also like to thank the following people for their assistance with field management, data collection, and analysis: Keith Kohler, Ben Knutson, Otis Smith, Richard Hartwig, Kevin Jensen, Wendy Borja and Jacob House. NR 77 TC 2 Z9 2 U1 35 U2 59 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD JUL 1 PY 2016 VL 172 BP 40 EP 50 DI 10.1016/j.agwat.2016.04.006 PG 11 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DO4GP UT WOS:000377740100005 ER PT J AU Yakubova, G Kavetskiy, A Prior, SA Torbert, HA AF Yakubova, Galina Kavetskiy, Aleksandr Prior, Stephen A. Torbert, H. Allen TI Application of Geant4 simulation for analysis of soil carbon inelastic neutron scattering measurements SO APPLIED RADIATION AND ISOTOPES LA English DT Article DE Monte-Carlo simulation; Geant4; Inelastic neutron scattering; Neutron generator; Soil carbon analysis ID MONTE-CARLO; SYSTEM; IMPACT AB Inelastic neutron scattering (INS) was applied to determine soil carbon content. Due to non-uniform soil carbon depth distribution, the correlation between INS signals with some soil carbon content parameter is not obvious; however, a proportionality between INS signals and average carbon weight percent in similar to 10 cm layer for any carbon depth profile is demonstrated using Monte-Carlo simulation (Geant4). Comparison of INS and dry combustion measurements confirms this conclusion. Thus, INS measurements give the value of this soil carbon parameter. Published by Elsevier Ltd. C1 [Yakubova, Galina; Kavetskiy, Aleksandr; Prior, Stephen A.; Torbert, H. Allen] USDA ARS, Natl Soil Dynam Lab, 411 South Donahue Dr, Auburn, AL 36832 USA. RP Yakubova, G (reprint author), USDA ARS, Natl Soil Dynam Lab, 411 South Donahue Dr, Auburn, AL 36832 USA. EM galina.yakubova@ars.usda.gov NR 33 TC 0 Z9 0 U1 1 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0969-8043 J9 APPL RADIAT ISOTOPES JI Appl. Radiat. Isot. PD JUL PY 2016 VL 113 BP 33 EP 39 DI 10.1016/j.apradiso.2016.04.013 PG 7 WC Chemistry, Inorganic & Nuclear; Nuclear Science & Technology; Radiology, Nuclear Medicine & Medical Imaging SC Chemistry; Nuclear Science & Technology; Radiology, Nuclear Medicine & Medical Imaging GA DO4FP UT WOS:000377737500007 PM 27124122 ER PT J AU Green, BW Rawles, SD Fuller, SA Beck, BH McEntire, ME AF Green, Bartholomew W. Rawles, Steven D. Fuller, Sidney A. Beck, Benjamin H. McEntire, Matthew E. TI Hypoxia affects performance traits and body composition of juvenile hybrid striped bass (Morone chrysops x M. saxatilis) SO AQUACULTURE RESEARCH LA English DT Article DE dissolved oxygen; hybrid striped bass; hypoxia; Morone; nutrient retention; nutrient utilization ID DISSOLVED-OXYGEN CONCENTRATIONS; COD GADUS-MORHUA; CHANNEL CATFISH; SEA BASS; DICENTRARCHUS-LABRAX; ATLANTIC COD; MESSENGER-RNA; EARTHEN PONDS; BROOK TROUT; FISH PONDS AB Performance traits and body composition of juvenile hybrid striped bass (Morone chrysops x M. saxatilis) in response to hypoxia were evaluated in replicate tanks maintained at constant dissolved oxygen concentrations that averaged 23.0 +/- 2.3%, 39.7 +/- 3.0% and 105.5 +/- 9.5% dissolved oxygen saturation. Fish were fed a commercially formulated feed daily to apparent satiation. Total feed intake and fish growth and yield increased linearly in response to increased dissolved oxygen concentration. Nutrient utilization was reduced significantly only at the greatest level of hypoxia. With the exception of whole body protein content, whole body compositional indices and nutrient retention efficiencies were linearly related to dissolved oxygen concentration. Results demonstrate that as hypoxia becomes more severe, juvenile hybrid striped bass feed intake is reduced, which affects growth and nutrient retention. C1 [Green, Bartholomew W.; Rawles, Steven D.; Fuller, Sidney A.; Beck, Benjamin H.; McEntire, Matthew E.] ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, POB 1050,2955 Highway 130 East, Stuttgart, AR 72160 USA. RP Green, BW (reprint author), ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, POB 1050,2955 Highway 130 East, Stuttgart, AR 72160 USA. EM bart.green@ars.usda.gov FU USDA-ARS [6225-31630-006-00D] FX We thank Greg O'Neal and Rebecca Jacobs for their assistance throughout these studies, and Dr Catherine Childress and Matt Barnett for their assistance during harvest. The authors have no conflict of interest to declare. This study was funded by the USDA-ARS under project number 6225-31630-006-00D. Mention of trade names or commercial products in this article is solely for providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 41 TC 1 Z9 1 U1 10 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1355-557X EI 1365-2109 J9 AQUAC RES JI Aquac. Res. PD JUL PY 2016 VL 47 IS 7 BP 2266 EP 2275 DI 10.1111/are.12678 PG 10 WC Fisheries SC Fisheries GA DO5AD UT WOS:000377795100021 ER PT J AU Vasantha-Srinivasan, P Senthil-Nathan, S Thanigaivel, A Edwin, ES Ponsankar, A Selin-Rani, S Pradeepa, V Sakthi-Bhagavathy, M Kalaivani, K Hunter, WB Duraipandiyan, V Al-Dhabi, NA AF Vasantha-Srinivasan, Prabhakaran Senthil-Nathan, Sengottayan Thanigaivel, Annamalai Edwin, Edward-Sam Ponsankar, Athirstam Selin-Rani, Selvaraj Pradeepa, Venkatraman Sakthi-Bhagavathy, Muthiah Kalaivani, Kandaswamy Hunter, Wayne B. Duraipandiyan, Veeramuthu Al-Dhabi, Naif Abdullah TI Developmental response of Spodoptera litura Fab. to treatments of crude volatile oil from Piper betle L. and evaluation of toxicity to earthworm, Eudrilus eugeniae Kinb. SO CHEMOSPHERE LA English DT Article DE Botanicals; Essential oil; Insect; GC-MS; Toxicity; Mortality; Histology; Earthworm ID LEPIDOPTERA-NOCTUIDAE; EISENIA-FOETIDA; INSECTICIDAL ACTIVITY; BACILLUS-SUBTILIS; CROSS-RESISTANCE; RELATIVE FITNESS; TOBACCO CUTWORM; EXTRACTS; SOIL; NUCLEOPOLYHEDROVIRUS AB Evaluations of biological effects of (Pb-CVO) the crude volatile oil of Piper betle leaves on the tobacco cutworm Spodoptera litura were conducted. Pb-CVO was subjected to GC-MS analysis and twenty vital compounds were isolated from the betel leaf oil. Pb-CVO was tested at four different concentrations (0.25, 0.5, 1.0 and 1.5%) against S. litura. The treated insects exhibited dose depended mortality. The mortality rate was significantly higher at the 1.0 and 1.5% Pb-CVO. The LC50 (Lethal concentration) were observed at 0.48% Pb-CVO. Larval and pupal durations increased in all treatment concentrations (0.25, 03, 0.4 and 0.5%) whereas, pupal weight decreased compared to control. Adult longevity of S. litura was reduced in all treatments but predominantly in the 0.4 and 0.5% Pb-CVO. Correspondingly, mean fecundity rate was reduced at all concentrations compared to control. Histological studies of larvae mid gut profiles of S. litura were severely damaged in 1.0 and 1.5% and showed abnormalities in mid-gut cells with 0.25 and 0.5% Pb-CVO treatments. Earthworm toxicity illustrated that 0.1% of chemical insecticides (monocrotophos and cypermethrin) varied widely in their contact toxicities compared to 0.5 and 1.0% Pb-CVO and control in both contact filter paper and artificial soil test. These findings suggest that twenty essential compounds of betel leaf oil were significant inhibitors of the development and caused behavioral changes of S. litura. Treatment with betel leaf oil at these concentrations had no adverse effect on earthworm populations. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Vasantha-Srinivasan, Prabhakaran; Senthil-Nathan, Sengottayan; Thanigaivel, Annamalai; Edwin, Edward-Sam; Ponsankar, Athirstam; Selin-Rani, Selvaraj; Pradeepa, Venkatraman; Sakthi-Bhagavathy, Muthiah] Manonmaniam Sundaranar Univ, Sri Paramakalyani Ctr Excellence Environm Sci, Div Biopesticides & Environm Toxicol, Tirunelveli 627412, Tamil Nadu, India. [Kalaivani, Kandaswamy] Sri Parasakthi Coll Women, Post Grad & Res Dept Zool, Tirunelveli 627802, Tamil Nadu, India. [Hunter, Wayne B.] USDA, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. [Duraipandiyan, Veeramuthu; Al-Dhabi, Naif Abdullah] King Saud Univ, Coll Sci, Addiriya Chair Environm Studies, Dept Bot & Microbiol, POB 2455, Riyadh 11451, Saudi Arabia. RP Senthil-Nathan, S (reprint author), Manonmaniam Sundaranar Univ, Sri Paramakalyani Ctr Excellence Environm Sci, Div Biopesticides & Environm Toxicol, Tirunelveli 627412, Tamil Nadu, India. EM senthil@msuniv.ac.in OI Senthil Nathan, Sengottayan/0000-0003-0839-2021 FU King Saud University, through Vice Deanship of Research Chairs FX The project was full financially supported by King Saud University, through Vice Deanship of Research Chairs. NR 67 TC 2 Z9 2 U1 6 U2 16 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0045-6535 EI 1879-1298 J9 CHEMOSPHERE JI Chemosphere PD JUL PY 2016 VL 155 BP 336 EP 347 DI 10.1016/j.chemosphere.2016.03.139 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA DO4FB UT WOS:000377736100040 PM 27135695 ER PT J AU Bacha, F Gidding, SS AF Bacha, Fida Gidding, Samuel S. TI Cardiac Abnormalities in Youth with Obesity and Type 2 Diabetes SO CURRENT DIABETES REPORTS LA English DT Review DE Cardiac structure; Cardiac function; Pediatric obesity; Type 2 diabetes ID LEFT-VENTRICULAR MASS; INTIMA-MEDIA THICKNESS; CORONARY-ARTERY CALCIFICATION; IMPAIRED GLUCOSE-TOLERANCE; AMBULATORY BLOOD-PRESSURE; CHILDHOOD RISK-FACTORS; YOUNG-ADULTS CARDIA; LEFT ATRIAL SIZE; HEART-FAILURE; BODY-MASS AB Childhood obesity has been linked to cardiovascular disease (CVD) risk in adulthood. Of great concern is the expected increase in the population's CVD burden in relation to childhood obesity. This is compounded by the risk related to chronic hyperglycemia exposure in youth with type 2 diabetes. We herein provide an overview of the spectrum of early cardiovascular disease manifestation in youth with obesity and type 2 diabetes, in particular abnormalities in cardiac structure and function. Cardiac remodeling and adverse target organ damage is already evident in the pediatric age group in children with obesity and type 2 diabetes. This supports the importance of intensifying obesity prevention efforts and early intervention to treat comorbidities of obesity in the pediatric age group to prevent cardiac events in early adulthood. C1 [Bacha, Fida] Texas Childrens Hosp, Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. [Bacha, Fida] Texas Childrens Hosp, Baylor Coll Med, Div Pediat Endocrinol & Diabet, Houston, TX 77030 USA. [Gidding, Samuel S.] Alfred I DuPont Hosp Children, Nemours Cardiac Ctr, Wilmington, DE USA. RP Bacha, F (reprint author), Texas Childrens Hosp, Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA.; Bacha, F (reprint author), Texas Childrens Hosp, Baylor Coll Med, Div Pediat Endocrinol & Diabet, Houston, TX 77030 USA. EM fbacha@bcm.edu FU USDA/ARS; NIDDK; NHLBI FX The authors report grant funding during the preparation of this manuscript. Dr. Bacha received grant funding from the USDA/ARS and from the NIDDK (as co-investigator in the TODAY study). Dr. Gidding received grant funding from the NHLBI and from the NIDDK (as co-investigator in the TODAY study), and he also developed educational material regarding familial hypercholesterolemia for the American College of Cardiology. NR 112 TC 0 Z9 0 U1 1 U2 5 PU CURRENT MEDICINE GROUP PI PHILADELPHIA PA 400 MARKET STREET, STE 700, PHILADELPHIA, PA 19106 USA SN 1534-4827 EI 1539-0829 J9 CURR DIABETES REP JI Curr. Diabetes Rep. PD JUL PY 2016 VL 16 IS 7 AR 62 DI 10.1007/s11892-016-0750-6 PG 9 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DO7CB UT WOS:000377938700007 PM 27168062 ER PT J AU Ruane, AC Hudson, NI Asseng, S Camarrano, D Ewert, F Martre, P Boote, KJ Thorburn, PJ Aggarwal, PK Angulo, C Basso, B Bertuzzi, P Biernath, C Brisson, N Challinor, AJ Doltra, J Gayler, S Goldberg, R Grant, RF Heng, L Hooker, J Hunt, LA Ingwersen, J Izaurralde, RC Kersebaum, KC Kumar, SN Muller, C Nendel, C O'Leary, G Olesen, JE Osborne, TM Palosuo, T Priesack, E Ripoche, D Rotter, RP Semenov, MA Shcherbak, I Steduto, P Stockle, CO Stratonovitch, P Streck, T Supit, I Tao, FL Travasso, M Waha, K Wallach, D White, JW Wolf, J AF Ruane, Alex C. Hudson, Nicholas I. Asseng, Senthold Camarrano, Davide Ewert, Frank Martre, Pierre Boote, Kenneth J. Thorburn, Peter J. Aggarwal, Pramod K. Angulo, Carlos Basso, Bruno Bertuzzi, Patrick Biernath, Christian Brisson, Nadine Challinor, Andrew J. Doltra, Jordi Gayler, Sebastian Goldberg, Richard Grant, Robert F. Heng, Lee Hooker, Josh Hunt, Leslie A. Ingwersen, Joachim Izaurralde, Roberto C. Kersebaum, Kurt Christian Kumar, Soora Naresh Mueller, Christoph Nendel, Claas O'Leary, Garry Olesen, Jorgen E. Osborne, Torn M. Palosuo, Taru Priesack, Eckart Ripoche, Dominique Roetter, Reimund P. Semenov, Mikhail A. Shcherbak, Iurii Steduto, Pasquale Stoeckle, Claudio O. Stratonovitch, Pierre Streck, Thilo Supit, Iwan Tao, Fulu Travasso, Maria Waha, Katharina Wallach, Daniel White, Jeffrey W. Wolf, Joost TI Multi-wheat-model ensemble responses to interannual climate variability SO ENVIRONMENTAL MODELLING & SOFTWARE LA English DT Article DE Crop modeling; Uncertainty; Multi-model ensemble; Wheat; AgMIP; Climate impacts; Temperature; Precipitation; lnterannual variability ID SIMULATION-MODEL; CROP MODEL; NITROGEN DYNAMICS; WINTER-WHEAT; LARGE-AREA; SYSTEMS SIMULATION; FARMING SYSTEMS; YIELD RESPONSE; GROWTH; WATER AB We compare 27 wheat models' yield responses to interannual climate variability, analyzed at locations in Argentina, Australia, India, and The Netherlands as part of the Agricultural Model Intercomparison and Improvement Project (AgMIP) Wheat Pilot. Each model simulated 1981-2010 grain yield, and we evaluate results against the interannual variability of growing season temperature, precipitation, and solar radiation. The amount of information used for calibration has only a minor effect on most models' climate response, and even small multi-model ensembles prove beneficial. Wheat model clusters reveal common characteristics of yield response to climate; however models rarely share the same cluster at all four sites indicating substantial independence. Only a weak relationship (R-2 <= 0.24) was found between the models' sensitivities to interannual temperature variability and their response to long-term warming, suggesting that additional processes differentiate climate change impacts from observed climate variability analogs and motivating continuing analysis and model development efforts. Published by Elsevier Ltd. C1 [Ruane, Alex C.] NASA, Goddard Inst Space Studies, New York, NY 10025 USA. [Hudson, Nicholas I.; Goldberg, Richard] Columbia Univ, Ctr Climate Syst Res, New York, NY USA. [Asseng, Senthold; Camarrano, Davide; Boote, Kenneth J.] Univ Florida, Agr & Biol Engn Dept, Gainesville, FL USA. [Camarrano, Davide] James Hutton Inst, Invergowrie, Dundee, Scotland. [Ewert, Frank; Angulo, Carlos] Univ Bonn, Inst Crop Sci & Resource Conservat, D-53115 Bonn, Germany. [Martre, Pierre] Natl Inst Agr Res INRA, Genet Divers & Ecophysiol Cereals GDEC UMR1095, F-63100 Clermont Ferrand, France. [Martre, Pierre] INRA, Montpellier SupAgro, LEASE UMR759, F-34060 Montpellier, France. [Thorburn, Peter J.; Waha, Katharina] CSIRO, St Lucia, Qld 4067, Australia. [Aggarwal, Pramod K.] Int Water Management Inst, Consultat Grp Int Agr Res, Res Program Climate Change Agr & Food Secur, New Delhi 110012, India. [Basso, Bruno] Michigan State Univ, Dept Geol Sci, E Lansing, MI 48824 USA. [Basso, Bruno] Michigan State Univ, Kellogg Biol Stn, E Lansing, MI 48824 USA. [Bertuzzi, Patrick; Ripoche, Dominique] INRA, AgroClim US1116, F-84914 Avignon, France. [Biernath, Christian; Priesack, Eckart] German Res Ctr Environm Hlth, Inst Biochem Plant Pathol, Helmholtz Zentrum Munchen, D-85764 Neuherberg, Germany. [Brisson, Nadine] INRA, Agron UMR0211, F-78750 Thiverval Grignon, France. [Brisson, Nadine] AgroParisTech, Agron UMR0211, F-78750 Thiverval Grignon, France. [Challinor, Andrew J.] Univ Leeds, Inst Climate & Atmospher Sci, Sch Earth & Environm, Leeds LS2 9JT, W Yorkshire, England. [Challinor, Andrew J.] Ctr Int Agr Trop, CGIAR ESSP Program Climate Change Agr & Food Secu, Cali 763537, Colombia. [Doltra, Jordi] Cantabrian Agr Res & Training Ctr, Muriedas 39600, Spain. [Gayler, Sebastian; Ingwersen, Joachim; Streck, Thilo] Univ Stuttgart Hohenheim, Inst Soil Sci & Land Evaluat, D-70599 Stuttgart, Germany. [Grant, Robert F.] Univ Alberta, Dept Renewable Resources, Edmonton, AB T6G 2E3, Canada. [Heng, Lee] IAEA, A-1400 Vienna, Austria. [Hooker, Josh] Univ Reading, Sch Agr Policy & Dev, Reading RG6 6AR, Berks, England. [Hunt, Leslie A.] Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada. [Izaurralde, Roberto C.] Univ Maryland, Dept Geog Sci, College Pk, MD 20782 USA. [Ewert, Frank; Kersebaum, Kurt Christian; Nendel, Claas] Leibniz Ctr Agr Landscape Res ZALF, Inst Landscape Syst Anal, D-15374 Muncheberg, Germany. [Kumar, Soora Naresh] Indian Agr Res Inst, Ctr Environm Sci & Climate Resilient Agr, New Delhi 110012, India. [Mueller, Christoph; Waha, Katharina] Potsdam Inst Climate Impact Res, D-14473 Potsdam, Germany. [O'Leary, Garry] Landscape & Water Sci, Dept Primary Ind, Horsham, Vic 3400, Australia. [Olesen, Jorgen E.] Aarhus Univ, Dept Agroecol, DK-8830 Tjele, Denmark. [Osborne, Torn M.] Univ Reading, Dept Meteorol, Natl Ctr Atmospher Sci, Reading RG6 6BB, Berks, England. [Palosuo, Taru; Roetter, Reimund P.; Tao, Fulu] Nat Resources Inst Finland Luke, Environm Impacts Grp, FI-01370 Vantaa, Finland. [Semenov, Mikhail A.; Stratonovitch, Pierre] Rothamsted Res, Computat & Syst Biol Dept, Harpenden AL5 2JQ, Herts, England. [Shcherbak, Iurii] Queensland Univ Technol, Inst Future Environm, Brisbane, Qld 4000, Australia. [Steduto, Pasquale] UN, Food & Agr Org, Rome, Italy. [Stoeckle, Claudio O.] Washington State Univ, Biol Syst Engn, Pullman, WA 99164 USA. [Supit, Iwan] Wageningen Univ, Earth Syst Sci Climate Change & Adapt Land Use &, NL-6700 AA Wageningen, Netherlands. [Tao, Fulu] Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China. [Travasso, Maria] INTA CIRN, Inst Climate & Water, RA-1712 Castelar, Argentina. [Wallach, Daniel] INRA, Agrosyst & Dev Terr UMR1248, F-31326 Castanet Tolosan, France. [White, Jeffrey W.] USDA ARS, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Wolf, Joost] Wageningen Univ, Plant Prod Syst, NL-6700 AA Wageningen, Netherlands. [Roetter, Reimund P.] Univ Gottingen, D-37073 Gottingen, Germany. RP Ruane, AC (reprint author), NASA, Goddard Inst Space Studies, Climate Impacts Grp, 2880 Broadway, New York, NY 10025 USA. EM Alexander.C.Ruane@nasa.gov RI Thorburn, Peter/A-6884-2011; Mueller, Christoph/E-4812-2016; Palosuo, Taru/B-9593-2012; Martre, Pierre/G-5399-2013; Olesen, Jorgen/C-2905-2016; Challinor, Andrew/C-4992-2008; Doltra, Jordi/C-2106-2015; OI Wallach, Daniel/0000-0003-3500-8179; Mueller, Christoph/0000-0002-9491-3550; Palosuo, Taru/0000-0003-4322-3450; Martre, Pierre/0000-0002-7419-6558; Olesen, Jorgen/0000-0002-6639-1273; Challinor, Andrew/0000-0002-8551-6617; Kersebaum, Kurt Christian/0000-0002-3679-8427; Grant, Robert/0000-0002-8890-6231; Boote, Kenneth/0000-0002-1358-5496; Priesack, Eckart/0000-0002-5088-9528 FU USDA grant on behalf of the UK Department for International Development [202108]; NASA Earth Sciences Research Program [281945.02.03.03.96]; NASA Modeling, Analysis, and Prediction Program [509496.02.08.04.24]; US National Institute for Food and Agriculture [2011-68002-30191] FX We acknowledge the efforts of AgMIP Leaders Cynthia Rosenzweig, Jim Jones, John Antle, and Jerry Hatfield in their efforts to initiate the AgMIP Wheat Pilot and encourage explorations such as these. AgMIP coordination and climate activities were funded in part by a USDA grant on behalf of the UK Department for International Development (program code 202108). Support for many European participants in the AgMIP Wheat Pilot was provided by the Modelling European Agriculture with Climate Change for Food Security (MACSUR) knowledge hub within the Joint Research Programming Initiative on Agriculture, Food Security and Climate Change (FACCE-JPI). Alex Ruane's contributions were supported by the NASA Earth Sciences Research Program (#281945.02.03.03.96) and the NASA Modeling, Analysis, and Prediction Program (#509496.02.08.04.24). Participation of Claudio Stockle and Crop-Syst simulations were supported by the project Regional Approaches to Climate Change for Pacific Northwest Agriculture (REACCH-PNA), funded through award #2011-68002-30191 from the US National Institute for Food and Agriculture. NR 107 TC 2 Z9 2 U1 21 U2 39 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1364-8152 EI 1873-6726 J9 ENVIRON MODELL SOFTW JI Environ. Modell. Softw. PD JUL PY 2016 VL 81 BP 86 EP 101 DI 10.1016/j.envsoft.2016.03.008 PG 16 WC Computer Science, Interdisciplinary Applications; Engineering, Environmental; Environmental Sciences SC Computer Science; Engineering; Environmental Sciences & Ecology GA DO4CJ UT WOS:000377729100008 ER PT J AU Vieira, AR Grass, J Fedorka-Cray, PJ Plumblee, JR Tate, H Cole, DJ AF Vieira, A. R. Grass, J. Fedorka-Cray, P. J. Plumblee, J. R. Tate, H. Cole, D. J. TI Attribution of Salmonella enterica serotype Hadar infections using antimicrobial resistance data from two points in the food supply system SO EPIDEMIOLOGY AND INFECTION LA English DT Article DE Analysis of data; foodborne infections; surveillance ID UNITED-STATES; ANIMAL ORIGIN; DISEASE; SURVEILLANCE; COMMODITIES; MODEL AB A challenge to the development of foodborne illness prevention measures is determining the sources of enteric illness. Microbial subtyping source-attribution models attribute illnesses to various sources, requiring data characterizing bacterial isolate subtypes collected from human and food sources. We evaluated the use of antimicrobial resistance data on isolates of Salmonella enterica serotype Hadar, collected from ill humans, food animals, and from retail meats, in two microbial subtyping attribution models. We also compared model results when either antimicrobial resistance or pulsed-field gel electrophoresis (PFGE) patterns were used to subtype isolates. Depending on the subtyping model used, 68-96% of the human infections were attributed to meat and poultry food products. All models yielded similar outcomes, with 86% [95% confidence interval (CI) 80-91] to 91% (95% CI 88-96) of the attributable infections attributed to turkey, and 6% (95% CI 2-10) to 14% (95% CI 8-20) to chicken. Few illnesses (<3%) were attributed to cattle or swine. Results were similar whether the isolates were obtained from food animals during processing or from retail meat products. Our results support the view that microbial subtyping models are a flexible and robust approach for attributing Salmonella Hadar. C1 [Vieira, A. R.; Grass, J.; Cole, D. J.] Ctr Dis Control & Prevent, Enter Dis Epidemiol Branch, Atlanta, GA USA. [Fedorka-Cray, P. J.; Plumblee, J. R.] USDA ARS, Athens, GA 30613 USA. [Tate, H.] US FDA, Silver Spring, MD USA. RP Vieira, AR (reprint author), Ctr Dis Control & Prevent, Enter Dis Epidemiol Branch, Atlanta, GA USA. EM vht8@cdc.gov NR 20 TC 0 Z9 0 U1 5 U2 5 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0950-2688 EI 1469-4409 J9 EPIDEMIOL INFECT JI Epidemiol. Infect. PD JUL PY 2016 VL 144 IS 9 BP 1983 EP 1990 DI 10.1017/S0950268816000066 PG 8 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA DO5JV UT WOS:000377820300023 PM 26838291 ER PT J AU Bolton, MD Ebert, MK Faino, L Rivera-Varas, V de Jonge, R Van de Peer, Y Thomma, BPHJ Secor, GA AF Bolton, Melvin D. Ebert, Malaika K. Faino, Luigi Rivera-Varas, Viviana de Jonge, Ronnie Van de Peer, Yves Thomma, Bart P. H. J. Secor, Gary A. TI RNA-sequencing of Cercospora beticola DMI-sensitive and -resistant isolates after treatment with tetraconazole identifies common and contrasting pathway induction SO FUNGAL GENETICS AND BIOLOGY LA English DT Article DE Ergosterol; Sterol demethylation inhibitor; RTA1; Cell-membrane; Transformation; EC50; Tetraconazole ID 14-ALPHA-DEMETHYLASE GENE CYP51; AZOLE FUNGICIDE SENSITIVITY; SUGAR-BEET; MYCOSPHAERELLA-GRAMINICOLA; SACCHAROMYCES-CEREVISIAE; CANDIDA-ALBICANS; PENICILLIUM-DIGITATUM; PATHOGEN; YEAST; BIOSYNTHESIS AB Cercospora beticola causes Cercospora leaf spot of sugar beet. Cercospora leaf spot management measures often include application of the sterol demethylation inhibitor (DMI) class of fungicides. The reliance on DMIs and the consequent selection pressures imposed by their widespread use has led to the emergence of resistance in C. beticola populations. Insight into the molecular basis of tetraconazole resistance may lead to molecular tools to identify DMI-resistant strains for fungicide resistance management programs. Previous work has shown that expression of the gene encoding the DMI target enzyme (CYP51) is generally higher and inducible in DMI-resistant C beticola field strains. In this study, we extended the molecular basis of DMI resistance in this pathosystem by profiling the transcriptional response of two C. beticola strains contrasting for resistance to tetraconazole. A majority of the genes in the ergosterol biosynthesis pathway were induced to similar levels in both strains with the exception of CbCyp51, which was induced several-fold higher in the DMI-resistant strain. In contrast, a secondary metabolite gene cluster was induced in the resistance strain, but repressed in the sensitive strain. Genes encoding proteins with various cell membrane fortification processes were induced in the resistance strain. Site-directed and ectopic mutants of candidate DMI-resistance genes all resulted in significantly higher EC50 values than the wild type strain, suggesting that the cell wall and/or membrane modified as a result of the transformation process increased resistance to tetraconazole. Taken together, this study identifies important cell membrane components and provides insight into the molecular events underlying DMI resistance in C beticola. Published by Elsevier Inc. C1 [Bolton, Melvin D.; Ebert, Malaika K.] USDA ARS, No Crop Sci Lab, Fargo, ND 58105 USA. [Bolton, Melvin D.; Ebert, Malaika K.; Rivera-Varas, Viviana; Secor, Gary A.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58105 USA. [Ebert, Malaika K.; Faino, Luigi; Thomma, Bart P. H. J.] Wageningen Univ, Lab Phytopathol, NL-6700 AP Wageningen, Netherlands. [de Jonge, Ronnie; Van de Peer, Yves] Univ Ghent VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium. [de Jonge, Ronnie; Van de Peer, Yves] Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9000 Ghent, Belgium. RP Bolton, MD (reprint author), USDA ARS, No Crop Sci Lab, Fargo, ND 58105 USA. EM Melvin.Bolton@ars.usda.gov OI faino, luigi/0000-0002-6807-4191 FU USDA-ARS CRIS [3060-22000-047-00]; Beet Sugar Development Foundation; Sugar beet Research and Education Board of MN and ND FX We thank Keshav Birla and Jon Neubauer (USDA-ARS) for excellent technical assistance. M.D. Bolton is supported by USDA-ARS CRIS project 3060-22000-047-00 and grants from the Beet Sugar Development Foundation and the Sugar beet Research and Education Board of MN and ND. NR 67 TC 0 Z9 0 U1 4 U2 8 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1087-1845 EI 1096-0937 J9 FUNGAL GENET BIOL JI Fungal Genet. Biol. PD JUL PY 2016 VL 92 BP 1 EP 13 DI 10.1016/j.fgb.2016.04.003 PG 13 WC Genetics & Heredity; Mycology SC Genetics & Heredity; Mycology GA DO5SZ UT WOS:000377844400002 PM 27112724 ER PT J AU Im, CH Park, YH Hammel, KE Park, B Kwon, SW Ryu, H Ryu, JS AF Im, Chak Han Park, Young-Hoon Hammel, Kenneth E. Park, Bokyung Kwon, Soon Wook Ryu, Hojin Ryu, Jae-San TI Construction of a genetic linkage map and analysis of quantitative trait loci associated with the agronomically important traits of Pleurotus eryngii SO FUNGAL GENETICS AND BIOLOGY LA English DT Article DE Breeding; Genetic map; Trait marker; Pleurotus eryngii; QTL ID MARKER-ASSISTED SELECTION; AGARICUS-BISPORUS; BUTTON MUSHROOM; MOLECULAR MARKERS; AFLP MARKERS; GROWTH-RATE; MATING-TYPE; CAP COLOR; OSTREATUS; YIELD AB Breeding new strains with improved traits is a long-standing goal of mushroom breeders that can be expedited by marker-assisted selection (MAS). We constructed a genetic linkage map of Pleurotus eryngii based on segregation analysis of markers in postmeiotic monokaryons from KNR2312. In total, 256 loci comprising 226 simple sequence-repeat (SSR) markers, 2 mating-type factors, and 28 insertion/deletion (InDel) markers were mapped. The map consisted of 12 linkage groups (LGs) spanning 1047.8 cM, with an average interval length of 4.09 cM. Four independent populations (Pd3, Pd8, Pd14, and Pd15) derived from crossing between four monokaryons from KNR2532 as a tester strain and 98 monokaryons from KNR2312 were used to characterize quantitative trait loci (QTL) for nine traits such as yield, quality, cap color, and earliness. Using composite interval mapping (CIM), 71 QTLs explaining between 5.82% and 33.17% of the phenotypic variations were identified. Clusters of more than five QTLs for various traits were identified in three genomic regions, on LGs 1, 7 and 9. Regardless of the population, 6 of the 9 traits studied and 18 of the 71 QTLs found in this study were identified in the largest cluster, LG1, in the range from 65.4 to 110.4 cM. The candidate genes for yield encoding transcription factor, signal transduction, mycelial growth and hydrolase are suggested by using manual and computational analysis of genome sequence corresponding to QTL region with the highest likelihood odds (LOD) for yield. The genetic map and the QTLs established in this study will help breeders and geneticists to develop selection markers for agronomically important characteristics of mushrooms and to identify the corresponding genes. (C) 2016 Elsevier Inc. All rights reserved. C1 [Im, Chak Han; Park, Bokyung; Ryu, Jae-San] Gyeongsangnam Do Agr Res & Extens Serv, Environm Friendly Res Div, 570 Daesin Ro, Jinju 52733, South Korea. [Park, Young-Hoon] Pusan Natl Univ, Dept Hort Biosci, Milyang, South Korea. [Hammel, Kenneth E.] US Forest Serv, Madison, WI USA. [Kwon, Soon Wook] Pusan Natl Univ, Dept Plant Biosci, Milyang, South Korea. [Ryu, Hojin] Chungbuk Natl Univ, Dept Biol, Cheongju, South Korea. RP Ryu, JS (reprint author), Gyeongsangnam Do Agr Res & Extens Serv, Environm Friendly Res Div, 570 Daesin Ro, Jinju 52733, South Korea. EM chakhanim@epost.kr; kehammel@facstaff.wisc.edu; coolmush88@gmail.com RI Hammel, Kenneth/G-1890-2011 OI Hammel, Kenneth/0000-0002-2935-5847 FU Golden seed project (IPET), MIFAFF [213003-04-3-SBY20] FX This study was supported by a grant from the Golden seed project (IPET No. 213003-04-3-SBY20), MIFAFF. We are grateful to Dr. Ali Asjad for writing assistance and proof reading this article. NR 56 TC 1 Z9 1 U1 9 U2 13 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1087-1845 EI 1096-0937 J9 FUNGAL GENET BIOL JI Fungal Genet. Biol. PD JUL PY 2016 VL 92 BP 50 EP 64 DI 10.1016/j.fgb.2016.05.002 PG 15 WC Genetics & Heredity; Mycology SC Genetics & Heredity; Mycology GA DO5SZ UT WOS:000377844400007 PM 27166667 ER PT J AU Rautiainen, S Manson, JE Lichtenstein, AH Sesso, HD AF Rautiainen, Susanne Manson, JoAnn E. Lichtenstein, Alice H. Sesso, Howard D. TI Dietary supplements and disease prevention - a global overview SO NATURE REVIEWS ENDOCRINOLOGY LA English DT Review ID RANDOMIZED CONTROLLED-TRIAL; VITAMIN-D SUPPLEMENTATION; PLACEBO-CONTROLLED TRIAL; AGE-RELATED CATARACT; BETA-CAROTENE SUPPLEMENTATION; N-3 FATTY-ACIDS; ORAL MAGNESIUM SUPPLEMENTATION; MILD COGNITIVE IMPAIRMENT; BONE-MINERAL DENSITY; SERVICES TASK-FORCE AB Dietary supplements are widely used and offer the potential to improve health if appropriately targeted to those in need. Inadequate nutrition and micronutrient deficiencies are prevalent conditions that adversely affect global health. Although improvements in diet quality are essential to address these issues, dietary supplements and/or food fortification could help meet requirements for individuals at risk of deficiencies. For example, supplementation with vitamin A and iron in developing countries, where women of reproductive age, infants and children often have deficiencies; with folic acid among women of reproductive age and during pregnancy; with vitamin D among infants and children; and with calcium and vitamin D to ensure bone health among adults aged >= 65 years. Intense debate surrounds the benefits of individual high-dose micronutrient supplementation among well-nourished individuals because the alleged beneficial effects on chronic diseases are not consistently supported. Daily low-dose multivitamin supplementation has been linked to reductions in the incidence of cancer and cataracts, especially among men. Baseline nutrition is an important consideration in supplementation that is likely to modify its effects. Here, we provide a detailed summary of dietary supplements and health outcomes in both developing and developed countries to help guide decisions about dietary supplement recommendations. C1 [Rautiainen, Susanne; Manson, JoAnn E.; Sesso, Howard D.] Harvard Univ, Brigham & Womens Hosp, Dept Med, Div Prevent Med,Sch Med, Boston, MA 02215 USA. [Rautiainen, Susanne] Karolinska Inst, Inst Environm Med, S-17177 Stockholm, Sweden. [Manson, JoAnn E.; Sesso, Howard D.] Harvard Univ, TH Chan Sch Publ Hlth, Dept Epidemiol, Boston, MA 02215 USA. [Lichtenstein, Alice H.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Sesso, Howard D.] Harvard Univ, Brigham & Womens Hosp, Dept Med, Div Aging,Sch Med, Boston, MA 02215 USA. RP Manson, JE (reprint author), Harvard Univ, Brigham & Womens Hosp, Dept Med, Div Prevent Med,Sch Med, Boston, MA 02215 USA.; Manson, JE (reprint author), Harvard Univ, TH Chan Sch Publ Hlth, Dept Epidemiol, Boston, MA 02215 USA. EM jmanson@rics.bwh.harvard.edu FU COFAS 2 Marie Curie Fellowship, Stockholm, Sweden; NIH [HL34594, CA138962, HHSN268201100001C, R01 HL102122]; USDA [1950-51000-072-02S]; USDA/NIFA/AFRI [2011-03389]; AHRQ [HHSA290201200012I] FX S.R. has received funding from COFAS 2 Marie Curie Fellowship, Stockholm, Sweden. J.E.M. has received grant or research support from the NIH (HL34594, CA138962 and HHSN268201100001C) for the for the VITamin D and OmegA-3 TriaL (VITAL) and other research studies. A.H.L has received funding from USDA 1950-51000-072-02S, USDA/NIFA/AFRI 2011-03389 and AHRQ/Contract HHSA290201200012I. H.D.S. has received grant support from the NIH (R01 HL102122) related to work on the VITamin D and OmegA-3 TriaL (VITAL). NR 221 TC 4 Z9 4 U1 17 U2 50 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1759-5029 EI 1759-5037 J9 NAT REV ENDOCRINOL JI Nat. Rev. Endocrinol. PD JUL PY 2016 VL 12 IS 7 BP 407 EP 420 DI 10.1038/nrendo.2016.54 PG 14 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DO6LU UT WOS:000377895800006 PM 27150288 ER PT J AU Dawson-Hughes, B Mitchell, PJ Cooper, C Gordon, CM Rizzoli, R AF Dawson-Hughes, B. Mitchell, P. J. Cooper, C. Gordon, C. M. Rizzoli, R. TI Response to Fenton and Fenton: evidence does not support the alkaline diet SO OSTEOPOROSIS INTERNATIONAL LA English DT Letter ID OLDER MEN; POTASSIUM CITRATE; WOMEN; BICARBONATE; DENSITY; TRIAL C1 [Dawson-Hughes, B.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. [Mitchell, P. J.] Synth Med NZ Ltd, Auckland, New Zealand. [Mitchell, P. J.] Univ Notre Dame Australia, Sydney, NSW, Australia. [Cooper, C.] Univ Oxford, Nuffield Dept Orthopaed, NIHR Musculoskeletal Biomed Res Unit, Oxford, England. [Cooper, C.] Univ Southampton, Southampton Gen Hosp, MRC Lifecourse Epidemiol Unit, Southampton, Hants, England. [Gordon, C. M.] Cincinnati Childrens Hosp, Div Adolescent & Transit Med, Cincinnati, OH USA. [Rizzoli, R.] Univ Hosp Geneva, Div Bone Dis, Geneva, Switzerland. [Rizzoli, R.] Fac Med, Geneva, Switzerland. RP Dawson-Hughes, B (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM Bess.Dawson-Hughes@tufts.edu OI Mitchell, Paul/0000-0002-7439-0369 FU Medical Research Council [MC_UU_12011/1, MC_UP_A620_1014] NR 9 TC 0 Z9 0 U1 3 U2 4 PU SPRINGER LONDON LTD PI LONDON PA 236 GRAYS INN RD, 6TH FLOOR, LONDON WC1X 8HL, ENGLAND SN 0937-941X EI 1433-2965 J9 OSTEOPOROSIS INT JI Osteoporosis Int. PD JUL PY 2016 VL 27 IS 7 BP 2389 EP 2390 DI 10.1007/s00198-016-3505-y PG 2 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DO8AQ UT WOS:000378004800028 PM 26856583 ER PT J AU Hsu, PK Huang, LH Geib, SM Hsu, JC AF Hsu, Po-Kai Huang, Li-Hsin Geib, Scott M. Hsu, Ju-Chun TI Identification of a carboxylesterase associated with resistance to naled in Bactrocera dorsalis (Hendel) SO PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY LA English DT Article DE Naled; Carboxylesterase; Sequestration; Bactrocera dorsalis ID CONFERRING INSECTICIDE RESISTANCE; MYZUS-PERSICAE SULZER; ORIENTAL FRUIT-FLY; PEACH-POTATO APHID; ORGANOPHOSPHORUS INSECTICIDES; ACETYLCHOLINESTERASE GENE; CULEX-QUINQUEFASCIATUS; DIPTERA TEPHRITIDAE; FLIES; MECHANISMS AB Compared to other organophosphate-resistant and-susceptible (S) lines of Bactrocera dorsalis, the carboxylesterase (CBE) BdE5 in the naled-resistant (nal-r) line has been found to possess remarkable quantitative elevation. Our study attempts to identify the role of BdE5 in naled resistance, and we discovered several points of interest Firstly, activity staining on native PAGE revealed that the percentage of flies with intensive BdE5 bands in the nal-r line was substantially higher than in the S line, indicating that the BdE5 band correlates with naled susceptibility. Secondly, in vitro and in vivo inhibition assays showed that BdE5 was inhibited by naled in both lines; under diagnostic doses of naled, the overall extent of inhibition on CBEs was much greater in the S line than in the nal-r line. Thirdly, NanoLC-nanoESi-MS/MS analysis used the NCBI database to identify and annotate BdE5 as an esterase FE4-like (XP_011200445.1) in B. dorsalis. Fourthly, rapid amplification of cDNA ends was used to obtain the 2012-bp full-length BdE5 cDNA, which contained an open reading frame of 1770 bp and encoded a putative protein of 590 amino acid residues. Phylogenetic analysis revealed that BdE5 is a secreted beta-esterase (E Glade) closely related to CG6414 (NP_570089), a CBE in Drosophila melanogaster. Finally, our relative quantification real-time PCR data showed a significant elevation in transcript levels of the BdE5 gene in nal-r line. Our results confirmed that BdE5 is correlated with naled resistance and provides further understanding about the identification and molecular characteristics of BdE5 in B. dorsalis. (C) 2016 Elsevier Inc. All rights reserved. C1 [Hsu, Po-Kai; Hsu, Ju-Chun] Natl Taiwan Univ, Dept Entomol, Taipei 106, Taiwan. [Huang, Li-Hsin] Council Agr, Taiwan Agr Chem & Tox Subst Res Inst, Taichung 413, Taiwan. [Geib, Scott M.] USDA ARS, Trop Crop & Commod Protect Res Unit, Daniel K Inouye Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. RP Hsu, JC (reprint author), Natl Taiwan Univ, Dept Entomol, 1,Sec 4,Roosevelt Rd, Taipei 10617, Taiwan. EM juchun@ntu.edu.tw FU Ministry of Science and Technology (MOST), Republic of China, Taiwan [100-2313-B-002-019-MY3, 101-2321-B-225-001] FX The authors appreciate the help of Mr. K-H Hua in many protein experiments, and Mr. C-Y Yu and Miss C-Y Ho for fly feeding. The authors would like to thank the Ministry of Science and Technology (MOST), Republic of China, Taiwan, for the financial support under the contracts: 100-2313-B-002-019-MY3 and 101-2321-B-225-001. We would also like to acknowledge the Academia Sinica Common Mass Spectrometry Facilities located at the Institute of Biological Chemistry for the protein data analyzed by LTQ-Orbitrap XL hybrid mass spectrometer. NR 54 TC 1 Z9 1 U1 8 U2 18 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0048-3575 EI 1095-9939 J9 PESTIC BIOCHEM PHYS JI Pest. Biochem. Physiol. PD JUL PY 2016 VL 131 BP 24 EP 31 DI 10.1016/j.pestbp.2016.02.001 PG 8 WC Biochemistry & Molecular Biology; Entomology; Physiology SC Biochemistry & Molecular Biology; Entomology; Physiology GA DO6XG UT WOS:000377926200004 PM 27265823 ER PT J AU Irvin, MR Rotroff, DM Aslibekyan, S Zhi, DG Hidalgo, B Motsinger-Reif, A Marvel, S Srinivasasainagendra, V Claas, SA Buse, JB Straka, RJ Ordovas, JM Borecki, IB Guo, XQ Chen, IYD Rotter, JI Wagner, MJ Arnett, DK AF Irvin, Marguerite R. Rotroff, Daniel M. Aslibekyan, Stella Zhi, Degui Hidalgo, Bertha Motsinger-Reif, Alison Marvel, Skylar Srinivasasainagendra, Vinodh Claas, Steven A. Buse, John B. Straka, Robert J. Ordovas, Jose M. Borecki, Ingrid B. Guo, Xiuqing Chen, Ida Y. D. Rotter, Jerome I. Wagner, Michael J. Arnett, Donna K. TI A genome-wide study of lipid response to fenofibrate in Caucasians: a combined analysis of the GOLDN and ACCORD studies SO PHARMACOGENETICS AND GENOMICS LA English DT Article DE cholesterol; dyslipidemia; fenofibrate; genome-wide association study; lipid; lipoprotein; triglyceride ID CORONARY-ARTERY-DISEASE; DENSITY-LIPOPROTEIN CHOLESTEROL; TYPE-2 DIABETES-MELLITUS; OF-FUNCTION MUTATIONS; CARDIOVASCULAR-DISEASE; HEART-DISEASE; PLASMA TRIGLYCERIDE; DIET NETWORK; RISK; ASSOCIATION AB Background Fibrates are commonly prescribed for hypertriglyceridemia, but they also lower LDL cholesterol and increase HDL cholesterol. Large interindividual variations in lipid response suggest that some patients may benefit more than others and genetic studies could help identify such patients. Methods We carried out the first genome-wide association study of lipid response to fenofibrate using data from two well-characterized clinical trials: the Genetics of Lipid Lowering Drugs and Diet Network (GOLDN) Study and the Action to Control Cardiovascular Risk in Diabetes (ACCORD) Study. Genome-wide association study data from both studies were imputed to the 1000 Genomes CEU reference panel (phase 1). Lipid response was modeled as the log ratio of the post-treatment lipid level to the pretreatment level. Linear mixed models (GOLDN, N=813 from 173 families) and linear regression models (ACCORD, N=781) adjusted for pretreatment lipid level, demographic variables, clinical covariates, and ancestry were used to evaluate the association of genetic markers with lipid response. Among Caucasians, the results were combined using inverse-variance weighted fixed-effects meta-analyses. The main findings from the meta-analyses were examined in other ethnic groups from the HyperTG study (N=267 Hispanics) and ACCORD (N=83 Hispanics, 138 African Americans). Results A known lipid locus harboring the pre-B-cell leukemia homeobox 4 (PBX4) gene on chromosome 19 is important for LDL cholesterol response to fenofibrate (smallest P=1.5x10(-8)). The main results replicated with nominal statistical significance in Hispanics from ACCORD (P<0.05). Conclusion Future research should evaluate the usefulness of this locus to refine clinical strategies for lipid-lowering treatments. Copyright (C) 2016 Wolters Kluwer Health, Inc. All rights reserved. C1 [Irvin, Marguerite R.; Aslibekyan, Stella; Hidalgo, Bertha; Claas, Steven A.; Arnett, Donna K.] Univ Alabama Birmingham, Dept Epidemiol, 1530 3rd Ave South, Birmingham, AL 35294 USA. [Zhi, Degui; Srinivasasainagendra, Vinodh] Univ Alabama Birmingham, Dept Biostat, Birmingham, AL 35294 USA. [Rotroff, Daniel M.; Motsinger-Reif, Alison; Marvel, Skylar] N Carolina State Univ, Dept Stat, Raleigh, NC 27695 USA. [Buse, John B.] Univ N Carolina, UAB Biostat, Diabet Res Ctr, Sch Med, Chapel Hill, NC USA. [Wagner, Michael J.] Univ N Carolina, UAB Epidemiol, Ctr Pharmacogen & Individualized Therapy, Chapel Hill, NC USA. [Straka, Robert J.] Univ Minnesota, Dept Expt & Clin Pharmacol, Minneapolis, MN USA. [Ordovas, Jose M.] Tufts Univ, NCState Biostat, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Borecki, Ingrid B.] Washington Univ, Dept Genet, Div Stat Genom, St Louis, MO 63110 USA. [Guo, Xiuqing] Harbor UCLA Med Ctr, Lab Stat & Math Genet, Torrance, CA 90509 USA. [Chen, Ida Y. D.] Harbor UCLA Med Ctr, Lab Biochem Mol Phenotyping & Microarray, Torrance, CA 90509 USA. [Rotter, Jerome I.] Harbor UCLA Med Ctr, Inst Translat Genom & Populat Sci, Torrance, CA 90509 USA. RP Irvin, MR (reprint author), Univ Alabama Birmingham, Dept Epidemiol, 1530 3rd Ave South, Birmingham, AL 35294 USA. EM irvinr@uab.edu OI Claas, Steven/0000-0001-9789-8395 FU National Heart, Lung, and Blood Institute (NHLBI) [R01HL091357]; IAA [Y1-HC-9035, Y1-HC-1010]; NHLBI [RO1 HL110380, R01HL076771]; National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK); National Eye Institute (NEI); National Institute on Aging (NIA); Centers for Disease Control and Prevention (CDC); NCATS [UL1TR000124]; NIDDK [DK063491]; [N01-HC-95178]; [N01-HC-95179]; [N01-HC-95180]; [N01-HC-95181]; [N01-HC-95182]; [N01-HC-95183]; [N01-HC-95184] FX GOLDN was supported by the National Heart, Lung, and Blood Institute (NHLBI) R01HL091357. ACCORD was supported by Contract Nos. N01-HC-95178, N01-HC-95179, N01-HC-95180, N01-HC-95181, N01-HC-95182, N01-HC-95183, N01-HC-95184, IAA #Y1-HC-9035, and IAA #Y1-HC-1010, and grant RO1 HL110380 from the NHLBI, with additional support from the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), the National Eye Institute (NEI), the National Institute on Aging (NIA), and the Centers for Disease Control and Prevention (CDC). HyperTG was supported by NHLBI R01HL076771, with additional support from NCATS UL1TR000124 and NIDDK DK063491. NR 46 TC 0 Z9 0 U1 3 U2 5 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 1744-6872 EI 1744-6880 J9 PHARMACOGENET GENOM JI Pharmacogenet. Genomics PD JUL PY 2016 VL 26 IS 7 BP 324 EP 333 DI 10.1097/FPC.0000000000000219 PG 10 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Pharmacology & Pharmacy SC Biotechnology & Applied Microbiology; Genetics & Heredity; Pharmacology & Pharmacy GA DO3MA UT WOS:000377684500002 PM 27002377 ER PT J AU Schaeffer, SM Nakata, PA AF Schaeffer, Scott M. Nakata, Paul A. TI The expanding footprint of CRISPR/Cas9 in the plant sciences SO PLANT CELL REPORTS LA English DT Review DE Cas9; CRISPR; Crop improvement; Gene editing; Gene knock-in; Functional genomics ID RNA-GUIDED ENDONUCLEASE; TARGETED MUTAGENESIS; GENE-EXPRESSION; CAS SYSTEM; EPIGENETIC REGULATION; IMMUNE-SYSTEM; HUMAN-CELLS; TRANSCRIPTIONAL REGULATION; HOMOLOGOUS RECOMBINATION; CAENORHABDITIS-ELEGANS AB CRISPR/Cas9 has evolved and transformed the field of biology at an unprecedented pace. From the initial purpose of introducing a site specific mutation within a genome of choice, this technology has morphed into enabling a wide array of molecular applications, including site-specific transgene insertion and multiplexing for the simultaneous induction of multiple cleavage events. Efficiency, specificity, and flexibility are key attributes that have solidified CRISPR/Cas9 as the genome-editing tool of choice by scientists from all areas of biology. Within the field of plant biology, several CRISPR/Cas9 technologies, developed in other biological systems, have been successfully implemented to probe plant gene function and to modify specific crop traits. It is anticipated that this trend will persist and lead to the development of new applications and modifications of the CRISPR technology, adding to an ever-expanding collection of genome-editing tools. We envision that these tools will bestow plant researchers with new utilities to alter genome complexity, engineer site-specific integration events, control gene expression, generate transgene-free edited crops, and prevent or cure plant viral disease. The successful implementation of such utilities will represent a new frontier in plant biotechnology. C1 [Schaeffer, Scott M.; Nakata, Paul A.] Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. RP Nakata, PA (reprint author), Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM paul.nakata@ars.usda.gov FU US Department of Agriculture, Agricultural Research Service [58-3092-5-001] FX This work was supported by the US Department of Agriculture, Agricultural Research Service, under Cooperative Agreement Number 58-3092-5-001. NR 156 TC 3 Z9 3 U1 34 U2 66 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0721-7714 EI 1432-203X J9 PLANT CELL REP JI Plant Cell Reports PD JUL PY 2016 VL 35 IS 7 BP 1451 EP 1468 DI 10.1007/s00299-016-1987-x PG 18 WC Plant Sciences SC Plant Sciences GA DO7BD UT WOS:000377936300006 PM 27137209 ER PT J AU Roberts, DP Lakshman, DK McKenna, LF Emche, SE Maul, JE Bauchan, G AF Roberts, Daniel P. Lakshman, Dilip K. McKenna, Laurie F. Emche, Sarah E. Maul, Jude E. Bauchan, Gary TI Seed Treatment with Ethanol Extract of Serratia marcescens is Compatible with Trichoderma Isolates for Control of Damping-off of Cucumber Caused by Pythium ultimum SO PLANT DISEASE LA English DT Article ID AGROBACTERIUM-MEDIATED TRANSFORMATION; BIOLOGICAL-CONTROL; ENTEROBACTER-CLOACAE; BIOCONTROL AGENTS; FLUORESCENT PSEUDOMONAS; PHYTOPHTHORA-CAPSICI; ANTIFUNGAL ACTIVITY; RHIZOCTONIA-SOLANI; PLANT-DISEASES; ROOT-ROT AB Environmentally friendly control measures for soilborne plant pathogens are needed that are effective in different soils when applied alone or as components of an integrated disease control strategy. An ethanol extract of Serratia marcescens N4-5, when applied as a cucumber seed treatment, effectively suppressed damping-off caused by Pythium ultimum in potting mix and in a sandy loam soil. Plant stand associated with this treatment was similar to that of seed treated with the chemical pesticide Thiram in the sandy loam soil. The N4-5 ethanol extract did not consistently provide significant disease control in a loam soil. The N4-5 ethanol extract was compatible with two Trichoderma isolates, not affecting in vitro or in situ colonization of cucumber by these biological control fungi. Control of damping-off of cucumber was never diminished when this ethanol extract was applied as a seed treatment in combination with in-furrow application of the Trichoderma isolates, and disease control was improved in certain instances with these combinations in the loam soil. Data presented here indicate that the N4-5 ethanol extract is compatible with certain beneficial fungi, suggesting that this extract can be used as a component of integrated disease control strategies featuring biological control fungi. C1 [Roberts, Daniel P.; Lakshman, Dilip K.; McKenna, Laurie F.; Emche, Sarah E.; Maul, Jude E.] USDA ARS, Sustainable Agr Syst Lab, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Lakshman, Dilip K.] USDA ARS, Florist & Nursery Plants Res Unit, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Bauchan, Gary] USDA ARS, Electron & Confocal Microscopy Unit, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Roberts, DP (reprint author), USDA ARS, Sustainable Agr Syst Lab, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM dan.roberts@ars.usda.gov NR 73 TC 0 Z9 0 U1 5 U2 20 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2016 VL 100 IS 7 BP 1278 EP 1287 DI 10.1094/PDIS-09-15-1039-RE PG 10 WC Plant Sciences SC Plant Sciences GA DO2WJ UT WOS:000377641400003 ER PT J AU Hansen, ZR Knaus, BJ Tabima, JF Press, CM Judelson, HS Grunwald, NJ Smart, CD AF Hansen, Zachariah R. Knaus, Brian J. Tabima, Javier F. Press, Caroline M. Judelson, Howard S. Grunwald, Niklaus J. Smart, Christine D. TI SNP-Based Differentiation of Phytophthora infestans Clonal Lineages Using Locked Nucleic Acid Probes and High-Resolution Melt Analysis SO PLANT DISEASE LA English DT Article ID POTATO FAMINE PATHOGEN; REAL-TIME PCR; EASTERN UNITED-STATES; LATE BLIGHT; GENOME EVOLUTION; CENTRAL MEXICO; POPULATIONS; DNA; LNA; TOMATO AB Phytophthora infestans, the cause of the devastating late blight disease of potato and tomato, exhibits a clonal reproductive lifestyle in North America. Phenotypes such as fungicide sensitivity and host preference are conserved among individuals within clonal lineages, while substantial phenotypic differences can exist between lineages. Whole P. infestans genomes were aligned and single nucleotide polymorphisms (SNPs) identified as targets for the development of clonal-lineage-specific molecular diagnostic tools. Informative SNPs were used to develop high resolution melt (HRM) assays and locked nucleic acid (LNA) probes to differentiate lineage US-23, the predominant lineage in the Eastern United States for the past several years, from three other U.S. lineages. Three different primer pairs targeting one to three SNPs were capable of separating lineage US-23 from lineages US-8, US-11, and US-24 using HRM analysis. A fourth HRM primer pair targeted a highly variable genomic region containing nine polymorphisms within 63 bp. These primers separated US-23, US-11, and US-8 plus US-24 into three separate groups following HRM analysis but did not separate US-8 from US-24. Additionally, two LNA probes were designed to target a portion of the P. infestans genome containing two SNPs diagnostic for US-23. A single multiplex quantitative polymerase chain reaction assay containing both differentially labeled LNA probes differentiated individuals belonging to lineage US-23 from those belonging to US-8, US-11, and US-24. C1 [Hansen, Zachariah R.] Cornell Univ, Sch Integrat Plant Sci, Plant Pathol & Plant Microbe Biol Sect, NYSAES, Geneva, NY 14456 USA. [Knaus, Brian J.; Press, Caroline M.; Grunwald, Niklaus J.] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97331 USA. [Tabima, Javier F.; Grunwald, Niklaus J.] Oregon State Univ, Bot & Plant Pathol, Corvallis, OR 97331 USA. [Judelson, Howard S.] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. [Grunwald, Niklaus J.; Smart, Christine D.] Cornell Univ, Plant Pathol & Plant Microbe Biol Sect, Sch Integrat Plant Sci, Geneva, NY 14456 USA. RP Hansen, ZR (reprint author), Cornell Univ, Sch Integrat Plant Sci, Plant Pathol & Plant Microbe Biol Sect, NYSAES, Geneva, NY 14456 USA. OI Tabima, Javier/0000-0002-3603-2691; Grunwald, Niklaus/0000-0003-1656-7602 FU United States Department of Agriculture (USDA) [5358-22000-039-00D]; USDA National Institute of Food and Agriculture Grant [2011-68004-30154] FX This work was supported, in part, by the United States Department of Agriculture (USDA) Agricultural Research Service Grant 5358-22000-039-00D (N. J. Grunwald) and USDA National Institute of Food and Agriculture Grant 2011-68004-30154 (H. S. Judelson, C. D. Smart, and N. J. Grunwald). We thank W. Fry (Cornell University) for providing isolates which were sent to his lab for genotyping, and the many researchers and growers across the country who continue to collect and submit isolates. NR 40 TC 0 Z9 0 U1 6 U2 14 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2016 VL 100 IS 7 BP 1297 EP 1306 DI 10.1094/PDIS-11-15-1247-RE PG 10 WC Plant Sciences SC Plant Sciences GA DO2WJ UT WOS:000377641400005 ER PT J AU Torres, SV Vargas, MM Godoy-Lutz, G Porch, TG Beaver, JS AF Torres, Suheidy Valentin Vargas, Maria M. Godoy-Lutz, Graciela Porch, Timothy G. Beaver, James S. TI Isolates of Rhizoctonia solani Can Produce both Web Blight and Root Rot Symptoms in Common Bean (Phaseolus vulgaris L.) SO PLANT DISEASE LA English DT Article ID ANASTOMOSIS GROUP; SUGAR-BEET; MOLECULAR MARKERS; NEW-YORK; RESISTANCE; IDENTIFICATION; SOIL; DIVERSITY; FUNGI; VIRULENCE AB In common bean (Phaseolus vulgaris L.), Rhizoctonia solani Kuhn is an important pathogen causing web blight (WB) in the tropics, and it is also a soilborne pathogen causing root rot (RR) worldwide. This pathogen is a species complex classified into 14 anastomosis groups (AG). AG 1-IA, AG 1-IB, AG 1-IE, AG 1-IF, AG 2-2, and AG 4 have been reported to cause WB of the aboveground structures of the plant, while AG 4 and AG 2-2 have been associated with RR. There is limited information, however, concerning the ability of particular isolates of specific AG to cause both diseases in common bean. Nine R. solani isolates, including three AG 1 and three AG 4 WB isolates and three AG 4 RR isolates collected from both leaves and roots, respectively, of common bean in Puerto Rico, were used to evaluate the response of 12 common bean genotypes to WB inoculated using a detached-leaf method and to RR inoculated using a solution suspension of R. solani mycelia in the greenhouse. All R. solani isolates were able to induce both RR and WB symptoms. RR readings were generally more severe than the WB readings. The RR isolate RR1 (AG 4) produced the most severe RR scores. A few bean lines had mean RR scores <= 4.4 for specific R. solani isolates on a scale of 1 to 9, with 1 representing resistant and 9 highly susceptible. However, all of the bean lines had mean RR scores >= 5.0 when inoculated with the isolates RR1, RR2, and RR3, which were determined to be AG 4 in this study. Significant line isolate interactions were observed for the WB and RR inoculations for the three planting dates, suggesting a differential response of the common bean lines to the pathogen. This genotypic interaction may require bean breeders and pathologists to monitor the virulence patterns of R. solani in specific growing environments, while the compatibility of specific R. solani isolates to both aerial and root tissue needs to be considered for disease control strategies. C1 [Torres, Suheidy Valentin; Vargas, Maria M.] Univ Puerto Rico, Dept Biol, Mayaguez, PR 00681 USA. [Godoy-Lutz, Graciela] Inst Dominicano Invest Agr & Forestales, Estn Expt Arroyo Loro, Km 5,Carretera San Juan Las Matas de Fafan, San Juan De La Maguana, Dominican Rep. [Porch, Timothy G.] USDA ARS, Trop Agr Res Stn, Mayaguez, PR 00680 USA. [Beaver, James S.] Univ Puerto Rico, Dept Agroenvironm Sci, Mayaguez, PR 00681 USA. RP Porch, TG (reprint author), USDA ARS, Trop Agr Res Stn, Mayaguez, PR 00680 USA. EM timothy.porch@ars.usda.gov NR 56 TC 0 Z9 0 U1 5 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2016 VL 100 IS 7 BP 1351 EP 1357 DI 10.1094/PDIS-11-15-1270-RE PG 7 WC Plant Sciences SC Plant Sciences GA DO2WJ UT WOS:000377641400011 ER PT J AU Strausbaugh, CA Wenninger, EJ Eujayl, IA AF Strausbaugh, Carl A. Wenninger, Erik J. Eujayl, Imad A. TI Length of Efficacy for Control of Curly Top in Sugar Beet With Seed and Foliar Insecticides SO PLANT DISEASE LA English DT Article ID BEMISIA-TABACI HOMOPTERA; WESTERN UNITED-STATES; PEACH-POTATO APHID; NEONICOTINOID INSECTICIDES; SYSTEMIC INSECTICIDES; NILAPARVATA-LUGENS; BROWN PLANTHOPPER; MYZUS-PERSICAE; IRAN-VIRUS; RESISTANCE AB Curly top in sugar beet caused by Beet curly top virus (BCTV) is an important yield-limiting disease that can be reduced via neonicotinoid and pyrethroid insecticides. The length of efficacy of these insecticides is poorly understood; therefore, field experiments were conducted with the seed treatment Poncho Beta (clothianidin at 60 g a.i. + beta-cyfluthrin at 8 g a.i. per 100,000 seed) and foliar treatment Asana (esfenvalerate at 55.48 g a.i./ha). A series of four experiments at different locations in the same field were conducted in 2014 and repeated in a neighboring field in 2015, with four treatments (untreated check, Poncho Beta, Asana, and Poncho Beta + Asana) which were arranged in a randomized complete block design with eight replications. To evaluate efficacy, viruliferous (contain BCTV strains) beet leafhoppers were released 8, 9, 10, or 1 1 weeks after planting for each experiment, which corresponded to 1, 2, 3, and 4 weeks after Asana application. Over both years, in 30 of 32 observation dates for treatments with Poncho Beta and 14 of 16 observation dates for Asana, visual curly top ratings decreased an average of 41 and 24%, respectively, with insecticide treatments compared with the untreated check. Over both years, in eight of eight experiments for treatments with Poncho Beta and six of eight experiments for Asana, root yields increased an average of 39 and 32%, respectively, with treatment compared with the untreated check. Over both years, the Poncho Beta treatments increased estimated recoverable sucrose (ERS) yield by 75% compared with the untreated check for weeks 8 and 9. By week 10, only the Poncho Beta + Asana treatment led to increases in ERS in both years, while the influence of increasing host resistance may have made other treatments more difficult to separate. When considering curly top symptoms, root yield, and ERS among all weeks and years, there was a tendency for the insecticides in the Poncho Beta + Asana treatment to complement each other to improve efficacy. C1 [Strausbaugh, Carl A.; Eujayl, Imad A.] USDA ARS, NWISRL, Kimberly, ID 83341 USA. [Wenninger, Erik J.] Univ Idaho, Kimberly Res & Extens Ctr, Kimberly, ID 83341 USA. RP Strausbaugh, CA (reprint author), USDA ARS, NWISRL, Kimberly, ID 83341 USA. EM Carl.Strausbaugh@ars.usda.gov OI Strausbaugh, Carl/0000-0001-6773-1375 FU United States Department of Agriculture Current Research Information System [5368-21220-004-00D]; Amalgamated Sugar Company, LLC; Beet Sugar Development Foundation; Snake River FX These data support the objectives for the United States Department of Agriculture Current Research Information System project 5368-21220-004-00D. We thank the Amalgamated Sugar Company, LLC, Beet Sugar Development Foundation, and Snake River sugar beet growers for supporting our research work; and T. Keeth, D. Kenney, J. Reed, A. Stanzak, and A. Lockner for their technical support efforts. NR 64 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2016 VL 100 IS 7 BP 1364 EP 1370 DI 10.1094/PDIS-02-16-0142-RE PG 7 WC Plant Sciences SC Plant Sciences GA DO2WJ UT WOS:000377641400013 ER PT J AU Mahadevakumar, S Szabo, LJ Eilam, T Anikster, Y Janardhana, GR AF Mahadevakumar, S. Szabo, Les J. Eilam, T. Anikster, Y. Janardhana, G. R. TI A New Rust Disease on Wild Coffee (Psychotria nervosa) Caused by Puccinia mysuruensis sp nov. SO PLANT DISEASE LA English DT Article ID LIFE-CYCLE; FUNGI; IDENTIFICATION; MALVACEARUM; TELIOSPORE AB Psychotria nervosa, commonly called "wild coffee" (Rubiaceae), is an important ethno-medicinal plant in India. In 2010, a new rust disease of P. nervosa was observed in three regions of Mysore District, Karnataka (India), with disease incidence ranging from 58 to 63%.Typical symptoms of the rust disease on wild coffee were prominently visible during the early monsoon season (May to June), with chlorotic spots on the adaxial and black pustules (telia) on the abaxial leaf surface. Telia produced abundant teliospores, which were bicelled, pedicillate, and measured 33 to 45 by 19 to 30 mu m. The germination of teliospores produced a typical metabasidium bearing four basidiospores, each containing two haploid nuclei. Spore stages of the wild coffee rust pathogen were studied using artificially inoculated healthy wild coffee plants with germinated teliospores. Only telia were observed on the inoculated plants, indicating that this rust fungus has an abbreviated microcyclic life cycle that includes only teliospores and basidiospores. Phylogenetic analysis based on internal transcribed spacer and partial large subunit (LSU) sequence data showed that the wild coffee rust pathogen is related to Macruropyxis fraxini, Puccinia bartholomaei, P. choridis, and P. sparganioidis. The herbarium sample of P. psychotriae was examined and was shown to be different with respect to telium size and teliospore dimensions (24 to 32 by 13 to 18 mu m). Therefore, the rust pathogen causing wild coffee rust is a new species, P. mysuruensis sp. nov. C1 [Mahadevakumar, S.; Janardhana, G. R.] Univ Mysore, Dept Studies Bot, Mycol & Phytopathol Lab, Mysuru 570006, Karnataka, India. [Szabo, Les J.] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Szabo, Les J.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Eilam, T.; Anikster, Y.] Tel Aviv Univ, Dept Bot, IL-69978 Ramat Aviv, Israel. [Eilam, T.; Anikster, Y.] Tel Aviv Univ, Inst Cereal Crops Improvement, IL-69978 Ramat Aviv, Israel. RP Janardhana, GR (reprint author), Univ Mysore, Dept Studies Bot, Mycol & Phytopathol Lab, Mysuru 570006, Karnataka, India. EM grjbelur@gmail.com FU Department of Science and Technology, New Delhi, India [IF110422] FX We thank C. Manoharachary, Professor Emeritus, Osmania University, Hyderabad, India and K. R. Sridhar, UGC-BSR Faculty, Mangalore University, Mangalore, India for their help and critical comments; and K. P. Nguyen and A. Nemitz, United States Department of Agriculture-Agricultural Research Service Cereal Disease Laboratory, for technical assistance. S. Mahadevakumar (IF110422) acknowledges the Department of Science and Technology, New Delhi, India, for providing an Inspire Fellowship. NR 36 TC 0 Z9 0 U1 9 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2016 VL 100 IS 7 BP 1371 EP 1378 DI 10.1094/PDIS-07-15-0789-RE PG 8 WC Plant Sciences SC Plant Sciences GA DO2WJ UT WOS:000377641400014 ER PT J AU Newberry, EA Jardini, TM Rubio, I Roberts, PD Babu, B Koike, ST Bouzar, H Goss, EM Jones, JB Bull, CT Paret, ML AF Newberry, E. A. Jardini, T. M. Rubio, I. Roberts, P. D. Babu, B. Koike, S. T. Bouzar, H. Goss, E. M. Jones, J. B. Bull, C. T. Paret, M. L. TI Angular Leaf Spot of Cucurbits is Associated With Genetically Diverse Pseudomonas syringae Strains SO PLANT DISEASE LA English DT Article ID NEIGHBOR-JOINING METHOD; PLANT PATHOGEN; PV. SYRINGAE; HOST-RANGE; VIRULENCE; BLIGHT; CANTALOUPE; EXPRESSION; LACHRYMANS; EVOLUTION AB Angular leaf spot of cucurbits is generally considered to be caused by Pseudomonas syringae pv. lachrymans. It has a worldwide distribution and has been observed to emerge sporadically under humid and wet conditions. Reports of multiple P. syringae pathovars associated with the disease and lack of molecular analysis has left the true diversity of populations in the United States unclear. In this study, we collected 27 P. syringae strains causing foliar lesions and blighting on watermelon, cantaloupe, and squash in Florida, Georgia, and California over several years. Strains were fluorescent on King's medium B agar and displayed the typical phenotypic and biochemical characteristics of P. syringae. P. syringae pv. lachrymans is a member of genomospecies 2. However, the genetic profiles obtained through both MLSA (gyrB, rpoD, gapA, and gltA) and BOX-PCR (BOXA1R) identified 26 of the P. syringae strains to be distributed among three clades within genomospecies 1, and phylogenetically distinct from genomospecies 2 member P. syringae pv. lachrymans. A novel MLSA haplotype of the pathogen common to all states and cucurbit hosts was identified. Considerable genetic diversity among P. syringae strains infecting cucurbits is associated with the same disease, and reflects the larger ecological diversity of P. syringae populations from genomospecies 1. C1 [Newberry, E. A.; Babu, B.; Paret, M. L.] Univ Florida, North Florida Res & Educ Ctr, Quincy, FL 32351 USA. [Jardini, T. M.; Rubio, I.; Bull, C. T.] USDA ARS, Salinas, CA 93905 USA. [Rubio, I.] Calif State Univ, Undergrad Res Opportun Ctr, Seaside, Monterey, CA USA. [Roberts, P. D.] Univ Florida, Southwest Florida Res & Educ Ctr, Immokalee, FL USA. [Koike, S. T.] Univ Calif Cooperat Extens, Salinas, CA USA. [Bouzar, H.] Sakata Seed Amer Inc, Salinas, CA USA. [Jones, J. B.] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA. [Bull, C. T.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. RP Paret, ML (reprint author), Univ Florida, North Florida Res & Educ Ctr, Quincy, FL 32351 USA.; Bull, CT (reprint author), USDA ARS, Salinas, CA 93905 USA.; Bull, CT (reprint author), Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. EM carolee.bull@ars.usda.gov; paret@ufl.edu FU National Science Foundation [1012059]; US Department of Education; US Department of Agriculture; Florida Watermelon Association FX This research was supported by the National Science Foundation Small Business Innovative Research grant 1012059 and in part by the US Department of Education, the US Department of Agriculture, and the Florida Watermelon Association. Thanks to Dr. Ron Gitaitis, Dr. Bhabesh Dhutta, and Dr. David Langston for providing bacterial strains from Georgia and Polly H. Goldman for technical support. NR 46 TC 0 Z9 0 U1 14 U2 16 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2016 VL 100 IS 7 BP 1397 EP 1404 DI 10.1094/PDIS-11-15-1332-RE PG 8 WC Plant Sciences SC Plant Sciences GA DO2WJ UT WOS:000377641400017 ER PT J AU Sharma-Poudyal, D Paulitz, TC du Toit, LJ AF Sharma-Poudyal, Dipak Paulitz, Timothy C. du Toit, Lindsey J. TI Timing of Glyphosate Applications to Wheat Cover Crops to Reduce Onion Stunting Caused by Rhizoctonia solani SO PLANT DISEASE LA English DT Article ID ROOT-ROT; SUGAR-BEET; AG 8; PATHOGENS; HERBICIDE; VIRULENCE; AUSTRALIA; BARLEY; SPP.; SOIL AB Stunting caused by Rhizoctonia spp. is economically important in irrigated onion bulb crops in the semiarid Columbia Basin of Oregon and Washington, where cereal winter cover crops commonly are planted the previous fall to prevent wind erosion of soil. The cover crop is killed with herbicide application just before or shortly after onion seeding, so that the dead rows of cereal plants provide a physical barrier tall enough to protect onion seedlings against wind and sand blasting but not tall enough to shade onion seedlings. However, the cover crop also serves as a green bridge for Rhizoctonia spp. on cereal roots to colonize the onion roots, potentially resulting in severe stunting of onion seedlings. To determine the effect of timing of application of the herbicide glyphosate to reduce this green bridge effect and, subsequently, onion stunting, three herbicide application intervals preceding onion planting were evaluated in a grower's onion field in each of 2012 and 2014 in the Columbia Basin. The wheat cover crop was killed with a glyphosate application 27, 17, and 3 days before onion seeding in 2012 and 19, 10, and 3 days before seeding in 2014. As the interval between herbicide application and onion planting increased from 3 days to 19 and 27 days, the number of patches of stunted onion plants decreased by >= 55%, total area of stunted patches decreased by 54 to 63%, and patch severity index decreased by 59 to 65%. Similarly, the Rhizoctonia solani AG 8 DNA concentration in soil sampled from the dead cover crop rows declined as the interval between glyphosate application and onion seeding increased in the 2012 trial but not in the 2014 trial. R. solani AG 3 and AG 8 DNA concentrations in soil sampled from the cover crop rows were significantly positively correlated with the number of patches of stunted onion plants (r = 0.490 and 0.607 at P = 0.039 and 0.008, respectively), total area of stunted patches (r = 0.496 and 0.659 at P = 0.035 and 0.003, respectively), and patch severity index (r = 0.492 and 0.635 at P = 0.038 and 0.005, respectively) in the 2012 trial; however, these variables were only correlated significantly with R. solani AG 3 DNA concentration in the 2014 trial. Increasing the interval between herbicide application to the cover crop and onion planting provides a practical management tool for stunting in onion bulb crops. C1 [Sharma-Poudyal, Dipak] Washington State Univ, Pullman, WA 99164 USA. [Paulitz, Timothy C.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. [du Toit, Lindsey J.] Washington State Univ, Mt Vernon Northwestern Washington Res & Extens Ct, Mt Vernon, WA 98273 USA. [Sharma-Poudyal, Dipak] Oregon Dept Agr, Plant Hlth Program, Salem, OR 97301 USA. RP du Toit, LJ (reprint author), Washington State Univ, Mt Vernon Northwestern Washington Res & Extens Ct, Mt Vernon, WA 98273 USA. EM dutoit@wsu.edu OI Paulitz, Timothy/0000-0002-8885-3803 FU Washington State Department of Agriculture Specialty Crop Block Grant [K525]; PPNS [0700] FX This research was supported by the Washington State Department of Agriculture Specialty Crop Block Grant Number K525; and PPNS number 0700, Department of Plant Pathology, College of Agricultural, Human, & Natural Resource Sciences, Agricultural Research Center, Hatch Project Number WNP05595, Washington State University, Pullman, WA 99164-6430. NR 37 TC 0 Z9 0 U1 5 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2016 VL 100 IS 7 BP 1474 EP 1481 DI 10.1094/PDIS-10-15-1234-RE PG 8 WC Plant Sciences SC Plant Sciences GA DO2WJ UT WOS:000377641400027 ER PT J AU Kim, YK Kwak, JH Aguilar, CG Xiao, CL AF Kim, Y. K. Kwak, J. H. Aguilar, C. G. Xiao, C. L. TI First Report of Black Rot on Apple Fruit Caused by Diplodia seriata in Washington State. SO PLANT DISEASE LA English DT News Item C1 [Kim, Y. K.; Kwak, J. H.] Pace Int, Wapato, WA 98951 USA. [Aguilar, C. G.] Washington State Univ, TFREC, Wenatchee, WA 98801 USA. [Xiao, C. L.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Kim, YK (reprint author), Pace Int, Wapato, WA 98951 USA. NR 3 TC 0 Z9 0 U1 3 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2016 VL 100 IS 7 BP 1499 EP 1499 DI 10.1094/PDIS-12-15-1463-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DO2WJ UT WOS:000377641400048 ER PT J AU Menard, RD Clarke, SR Fraedrich, SW Harrington, TC AF Menard, R. D. Clarke, S. R. Fraedrich, S. W. Harrington, T. C. TI First Report of Laurel Wilt, Caused by Raffaelea lauricola, on Redbay (Persea borbonia) in Texas. SO PLANT DISEASE LA English DT News Item ID CRYPHONECTRIA-PARASITICA C1 [Menard, R. D.] US Forest Serv, Forest Hlth Protect, Pineville, LA 71360 USA. [Clarke, S. R.] US Forest Serv, Forestry Hlth Protect, Lufkin, TX 75901 USA. [Fraedrich, S. W.] US Forest Serv, Forestry Sci Lab, Athens, GA 30602 USA. [Harrington, T. C.] Iowa State Univ, Dept Plant Pathol & Microbiol, Ames, IA 50011 USA. RP Menard, RD (reprint author), US Forest Serv, Forest Hlth Protect, Pineville, LA 71360 USA. NR 4 TC 0 Z9 0 U1 3 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2016 VL 100 IS 7 BP 1502 EP 1503 DI 10.1094/PDIS-01-16-0038-PDN PG 2 WC Plant Sciences SC Plant Sciences GA DO2WJ UT WOS:000377641400057 ER PT J AU Mollov, D Tahir, MN Wei, C Kaye, C Lockhart, B Comstock, JC Rott, P AF Mollov, D. Tahir, M. N. Wei, C. Kaye, C. Lockhart, B. Comstock, J. C. Rott, P. TI First Report of Sugarcane mosaic virus Infecting Columbus Grass (Sorghum almum) in the United States. SO PLANT DISEASE LA English DT News Item C1 [Mollov, D.; Tahir, M. N.] USDA ARS, Natl Germplasm Resources Lab, Beltsville, MD 20705 USA. [Wei, C.; Rott, P.] Univ Florida, Everglades Res & Educ Ctr, IFAS, Dept Plant Pathol, Belle Glade, FL 33430 USA. [Kaye, C.] US Sugar Corp, Clewiston, FL 33440 USA. [Lockhart, B.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Comstock, J. C.] USDA ARS, Sugarcane Field Stn, Canal Point, FL 33438 USA. RP Mollov, D (reprint author), USDA ARS, Natl Germplasm Resources Lab, Beltsville, MD 20705 USA. NR 4 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2016 VL 100 IS 7 BP 1510 EP 1510 DI 10.1094/PDIS-01-16-0093-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DO2WJ UT WOS:000377641400077 ER PT J AU Chialva, M Zouari, I Salvioli, A Novero, M Vrebalov, J Giovannoni, JJ Bonfante, P AF Chialva, Matteo Zouari, Ines Salvioli, Alessandra Novero, Mara Vrebalov, Julia Giovannoni, James J. Bonfante, Paola TI Gr and hp-1 tomato mutants unveil unprecedented interactions between arbuscular mycorrhizal symbiosis and fruit ripening SO PLANTA LA English DT Article DE Arbuscular mycorrhiza; Ethylene; Light signaling; Ripening mutants; Tomato; Funneliformis mosseae ID TRANSPORTER GENES; ETHYLENE MUTANTS; PLANTS; EXPRESSION; QUALITY; COLONIZATION; ASSOCIATIONS; ARABIDOPSIS; TRANSCRIPTS; COOPERATION AB Systemic responses to an arbuscular mycorrhizal fungus reveal opposite phenological patterns in two tomato ripening mutants depending whether ethylene or light reception is involved. The availability of tomato ripening mutants has revealed many aspects of the genetics behind fleshy fruit ripening, plant hormones and light signal reception. Since previous analyses revealed that arbuscular mycorrhizal symbiosis influences tomato berry ripening, we wanted to test the hypothesis that an interplay might occur between root symbiosis and fruit ripening. With this aim, we screened seven tomato mutants affected in the ripening process for their responsiveness to the arbuscular mycorrhizal fungus Funneliformis mosseae. Following their phenological responses we selected two mutants for a deeper analysis: Green ripe (Gr), deficient in fruit ethylene perception and high-pigment-1 (hp-1), displaying enhanced light signal perception throughout the plant. We investigated the putative interactions between ripening processes, mycorrhizal establishment and systemic effects using biochemical and gene expression tools. Our experiments showed that both mutants, notwithstanding a normal mycorrhizal phenotype at root level, exhibit altered arbuscule functionality. Furthermore, in contrast to wild type, mycorrhization did not lead to a higher phosphate concentration in berries of both mutants. These results suggest that the mutations considered interfere with arbuscular mycorrhiza inducing systemic changes in plant phenology and fruits metabolism. We hypothesize a cross talk mechanism between AM and ripening processes that involves genes related to ethylene and light signaling. C1 [Chialva, Matteo; Zouari, Ines; Salvioli, Alessandra; Novero, Mara; Bonfante, Paola] Univ Turin, Dept Life Sci & Syst Biol, Viale Mattioli 25, I-10125 Turin, Italy. [Vrebalov, Julia; Giovannoni, James J.] Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA. [Giovannoni, James J.] USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY USA. RP Bonfante, P (reprint author), Univ Turin, Dept Life Sci & Syst Biol, Viale Mattioli 25, I-10125 Turin, Italy. EM paola.bonfante@unito.it OI Bonfante, Paola/0000-0003-3576-8530 FU Compagnia di San Paolo; Mycoplant Project (Progetto di Ateneo); Mycoplant Project (CSP); US Department of Agriculture, Agricultural Research Service FX The authors gratefully thank Dr. Agnese Giacomino (Department of Chemistry, University of Turin) for invaluable support during ICP-OES analysis and Dr. Maria Teresa Della Beffa (DBIOS, University of Turin) for maintaining tomato cultures in climatic chambers and greenhouses. We are deeply indebted with Katherine A. Borkovich for the critical reading and language editing of the final manuscript. The PhD fellowship to MC was funded by Compagnia di San Paolo. Research in the Bonfante lab was supported by the Mycoplant Project (Progetto di Ateneo and CSP). Activities in the Giovannoni lab were supported by the US Department of Agriculture, Agricultural Research Service. NR 51 TC 1 Z9 1 U1 8 U2 24 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0032-0935 EI 1432-2048 J9 PLANTA JI Planta PD JUL PY 2016 VL 244 IS 1 BP 155 EP 165 DI 10.1007/s00425-016-2491-9 PG 11 WC Plant Sciences SC Plant Sciences GA DO7BE UT WOS:000377936400011 PM 27002971 ER PT J AU Langendoen, EJ Mendoza, A Abad, JD Tassi, P Wang, DC Ata, R Abderrezzak, KE Hervouet, JM AF Langendoen, Eddy J. Mendoza, Alejandro Abad, Jorge D. Tassi, Pablo Wang, Dongchen Ata, Riadh Abderrezzak, Kamal El Kadi Hervouet, Jean-Michel TI Improved numerical modeling of morphodynamics of rivers with steep banks SO ADVANCES IN WATER RESOURCES LA English DT Article DE Bank erosion; Meander migration; Numerical model; TELEMAC ID COMPOUND MEANDER LOOP; CHANNEL; EROSION; FLOW; SIMULATION; MIGRATION; STABILITY; EVOLUTION; FAILURES; ILLINOIS AB The flow and sediment transport processes near steep streambanks, which are commonly found in meandering, braided, and anastomosing stream systems, exhibit complex patterns that produce intricate interactions between bed and bank morphologic adjustment. Increasingly, multi-dimensional computer models of riverine morphodynamics are used to aid in the study of these processes. A number of depth-averaged two-dimensional models are available to simulate morphologic adjustment of both bed and banks. Unfortunately, these models use overly simplified conceptual models of riverbank erosion, are limited by inflexible structured mesh systems, or are unable to accurately account for the flow and sediment transport adjacent to streambanks of arbitrary geometry. A new, nonlinear model is introduced that resolves these limitations. The model combines the river morphodynamics computer models TELEMAC-2D and SISYPHE of the open source TELEMAC-MASCARET suite of solvers with the bank erosion modules of the CONCEPTS channel evolution computer model. The performance of the new model is evaluated for meander-planform initiation and development. The most important findings are: (1) the model is able to simulate a much greater variety and complexity in meander wavelengths; (2) simulated meander development agrees closely with the unified bar-bend theory of Tubino and Seminara (1990); and (3) the rate of meander planform adjustment is greatly reduced if the wavelength of alternate bars is similar to that of meanders. Published by Elsevier Ltd. C1 [Langendoen, Eddy J.] ARS, USDA, Natl Sedimentat Lab, Oxford, MS USA. [Mendoza, Alejandro; Abad, Jorge D.] Univ Pittsburgh, Dept Civil & Environm Engn, Pittsburgh, PA USA. [Tassi, Pablo; Wang, Dongchen; Ata, Riadh; Abderrezzak, Kamal El Kadi; Hervouet, Jean-Michel] Natl Lab Hydraul & Environm LNHE, EDF R&D, Chatou, France. [Tassi, Pablo; Wang, Dongchen; Ata, Riadh; Abderrezzak, Kamal El Kadi; Hervouet, Jean-Michel] St Venant Lab Hydraul, Chatou, France. [Mendoza, Alejandro] Metropolitan Autonomous Univ, Dept Basic Sci & Engn, Lerma De Villada, Mexico. RP Langendoen, EJ (reprint author), ARS, USDA, Natl Sedimentat Lab, POB 1157, Oxford, MS USA. FU CONACYT-Mexico; EDF RD FX This research was performed within a framework of collaboration between the University of Pittsburgh, the US Department of Agriculture and EDF R&D. We thank CONACYT-Mexico and EDF R&D for financially supporting Dr. Alejandro Mendoza during his post-doctoral period at the University of Pittsburgh. The RVR Meander simulations were performed with help from Christian Frias and Davide Motta. Computational meshes were generated using the software Triangle (http://www.cs.cmu.edu/similar toquake/triangle.html). NR 50 TC 4 Z9 4 U1 8 U2 14 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0309-1708 EI 1872-9657 J9 ADV WATER RESOUR JI Adv. Water Resour. PD JUL PY 2016 VL 93 BP 4 EP 14 DI 10.1016/j.advwatres.2015.04.002 PN A PG 11 WC Water Resources SC Water Resources GA DN7YI UT WOS:000377295700002 ER PT J AU Delgado-Lista, J Perez-Martinez, P Garcia-Rios, A Alcala-Diaz, JF Perez-Caballero, AI Gomez-Delgado, F Fuentes, F Quintana-Navarro, G Lopez-Segura, F Ortiz-Morales, AM Delgado-Casado, N Yubero-Serrano, EM Camargo, A Marin, C Rodriguez-Cantalejo, F Gomez-Luna, P Ordovas, JM Lopez-Miranda, J Perez-Jimenez, F AF Delgado-Lista, Javier Perez-Martinez, Pablo Garcia-Rios, Antonio Alcala-Diaz, Juan F. Perez-Caballero, Ana I. Gomez-Delgado, Francisco Fuentes, Francisco Quintana-Navarro, Gracia Lopez-Segura, Fernando Ortiz-Morales, Ana M. Delgado-Casado, Nieves Yubero-Serrano, Elena M. Camargo, Antonio Marin, Carmen Rodriguez-Cantalejo, Fernando Gomez-Luna, Purificacion Ordovas, Jose M. Lopez-Miranda, Jose Perez-Jimenez, Francisco TI CORonary Diet Intervention with Olive oil and cardiovascular PREVention study (the CORDIOPREV study): Rationale, methods, and baseline characteristics: A clinical trial comparing the efficacy of a Mediterranean diet rich in olive oil versus a low-fat diet on cardiovascular disease in coronary patients SO AMERICAN HEART JOURNAL LA English DT Article ID FOOD-FREQUENCY QUESTIONNAIRE; HEART-DISEASE; RISK-FACTORS; METABOLIC SYNDROME; RANDOMIZED-TRIAL; INFLUENCE TRIGLYCERIDE; ARTERY DISEASE; HEALTH-STATUS; CLOCK GENE; ADHERENCE AB Coronary heart disease (CHD) represents a major global health burden. However, despite the well-known influence that dietary habits exert over the progression of this disease, there are no well-established and scientifically sound dietary approaches to prevent the onset of clinical outcomes in secondary prevention. The objective of the CORonary Diet Intervention with Olive oil and cardiovascular PREVention study (CORDIOPREV study, clinical trials number NCT00924937) is to compare the ability of a Mediterranean diet rich in virgin olive oil versus a low-fat diet to influence the composite incidence of cardiovascular events after 7 years in subjects with documented CHD at baseline. For this purpose, we enrolled 1,002 coronary patients from Spain. Baseline assessment (2009-2012) included detailed interviews and measurements to assess dietary, social, and biological variables. Results of baseline characteristics: The CORDIOPREV study in Spain describes a population with a high body mass index (37.2% overweight and 56.3% obesity) and with a median of low-density lipoprotein cholesterol of 88.5 mg/dL (70.6% of the patients having <100 mg/dL and 20.3% patients <70 mg/dL). A total of 9.6% of the participants were active smokers, and 64.4% were former smokers. Metabolic syndrome was present in 58% of this population. To sum up, we describe here the rationale, methods, and baseline characteristics of the CORDIOPREV study, which will test for the first time the efficacy of a Mediterranean diet rich in extra virgin olive oil as compared with a low-fat diet on the incidence of CHD recurrence in a long-term follow-up study. C1 [Delgado-Lista, Javier; Perez-Martinez, Pablo; Garcia-Rios, Antonio; Alcala-Diaz, Juan F.; Perez-Caballero, Ana I.; Gomez-Delgado, Francisco; Fuentes, Francisco; Quintana-Navarro, Gracia; Lopez-Segura, Fernando; Ortiz-Morales, Ana M.; Delgado-Casado, Nieves; Yubero-Serrano, Elena M.; Camargo, Antonio; Marin, Carmen; Gomez-Luna, Purificacion; Lopez-Miranda, Jose; Perez-Jimenez, Francisco] Univ Cordoba, Hosp Univ Reina Sofia, IMIBIC, Lipid & Atherosclerosis Unit, Cordoba, Spain. [Delgado-Lista, Javier; Perez-Martinez, Pablo; Garcia-Rios, Antonio; Alcala-Diaz, Juan F.; Perez-Caballero, Ana I.; Gomez-Delgado, Francisco; Fuentes, Francisco; Quintana-Navarro, Gracia; Lopez-Segura, Fernando; Ortiz-Morales, Ana M.; Delgado-Casado, Nieves; Yubero-Serrano, Elena M.; Camargo, Antonio; Marin, Carmen; Gomez-Luna, Purificacion; Lopez-Miranda, Jose; Perez-Jimenez, Francisco] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nutr CIBEROBN, Madrid, Spain. [Ordovas, Jose M.] CNIC, Dept Cardiovasc Epidemiol & Populat Genet, Madrid, Spain. [Ordovas, Jose M.] IMDEA Alimentac, Madrid, Spain. [Ordovas, Jose M.] Tufts Univ, Nutr & Genom Lab, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Rodriguez-Cantalejo, Fernando] Hosp Univ Reina Sofia, Biochem Lab, Cordoba 14004, Spain. RP Lopez-Miranda, J; Perez-Jimenez, F (reprint author), Reina Sofia Univ Hosp, IMIBIC Lipids & Atherosclerosis Res Unit, Ave Menendez Pidal S-N, Cordoba 14004, Spain. EM jlopezmir@uco.es; fperezjimenez@uco.es RI Yubero-Serrano, Elena /H-4832-2013 FU NHLBI NIH HHS [R01 HL054776] NR 44 TC 4 Z9 4 U1 11 U2 15 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0002-8703 EI 1097-5330 J9 AM HEART J JI Am. Heart J. PD JUL PY 2016 VL 177 BP 42 EP 50 DI 10.1016/j.ahj.2016.04.011 PG 9 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA DO0MN UT WOS:000377473100007 PM 27297848 ER PT J AU Kurtzman, CP AF Kurtzman, Cletus P. TI Description of Groenewaldozyma gen. nov for placement of Candida auringiensis, Candida salmanticensis and Candida tartarivorans SO ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY LA English DT Article DE Candida; Groenewaldozyma gen. nov.; Phylogenetics; Yeasts ID MULTIGENE PHYLOGENETIC ANALYSIS; WICKERHAMIELLA; F.A.; YEASTS; GENERA; COMBINATIONS; SEQUENCES AB DNA sequence analyses have demonstrated that species of the polyphyletic anamorphic ascomycete genus Candida may be members of described teleomorphic genera, members of the Candida tropicalis clade upon which the genus Candida is circumscribed, or members of isolated clades that represent undescribed genera. From phylogenetic analysis of gene sequences from nuclear large subunit rRNA, mitochondrial small subunit rRNA and cytochrome oxidase II, Candida auringiensis (NRRL Y-17674(T), CBS 6913(T)), Candida salmanticensis (NRRL Y-17090(T), CBS 5121(T)), and Candida tartarivorans (NRRL Y-27291(T), CBS 7955(T)) were shown to be members of an isolated clade and are proposed for reclassification in the genus Groenewaldozyma gen. nov. (MycoBank MB 815817). Neighbouring taxa include species of the Wickerhamiella clade and Candida blankii. C1 [Kurtzman, Cletus P.] ARS, USDA, Mycotoxin Prevent & Appl Microbiol Res Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. RP Kurtzman, CP (reprint author), ARS, USDA, Mycotoxin Prevent & Appl Microbiol Res Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM cletus.kurtzman@ars.usda.gov NR 20 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0003-6072 EI 1572-9699 J9 ANTON LEEUW INT J G JI Antonie Van Leeuwenhoek PD JUL PY 2016 VL 109 IS 7 BP 1041 EP 1045 DI 10.1007/s10482-016-0703-8 PG 5 WC Microbiology SC Microbiology GA DO0SW UT WOS:000377489600014 PM 27142089 ER PT J AU Yates, SR Ashworth, DJ Zheng, W Knuteson, J van Wesenbeeck, IJ AF Yates, S. R. Ashworth, D. J. Zheng, W. Knuteson, J. van Wesenbeeck, I. J. TI Effect of deep injection on field-scale emissions of 1,3-dichloropropene and chloropicrin from bare soil SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Soil fumigation; Emissions; 1,3-dichloropropene; Chloropicrin; Bare soil; Field experiment; Shank injection; Industrial Source Complex Short Term model (ISCST3); CALPUFF dispersion model; Aerodynamic gradient method; Integrated horizontal flux method; Theoretical profile shape method ID METHYL-BROMIDE EMISSIONS; FUMIGANT CHLOROPICRIN; NUMERICAL-SIMULATION; WATER APPLICATION; DIURNAL PATTERN; VOLATILIZATION; FLUX; MOISTURE; MODEL AB Fumigating soil is important for the production of many high-value vegetable, fruit, and tree crops, but fumigants are toxic pesticides with relatively high volatility, which can lead to significant atmospheric emissions. A field experiment was conducted to measure emissions and subsurface diffusion of a mixture of 1,3-dichloropropene (1,3-D) and chloropicrin after shank injection to bare soil at 61 cm depth (i.e., deep injection). Three on-field methods, the aerodynamic (ADM), integrated horizontal flux (IHF), and theoretical profile shape (TPS) methods, were used to obtain fumigant flux density and cumulative emission values. Two air dispersion models (CALPUFF and ISCST3) were also used to back-calculate the flux density using air concentration measurements surrounding the fumigated field. Emissions were continuously measured for 16 days and the daily peak emission rates for the five methods ranged from 13 to 33 mu g m(-2) s(-1) for 1,3-D and 0.22-3.2 mu g m(-2) s(-1) for chloropicrin. Total 1,3-D mass lost to the atmosphere was approximately 23-41 kg ha(-1), or 15-27% of the applied active ingredient and total mass loss of chloropicrin was <2%. Based on the five methods, deep injection reduced total emissions by approximately 2-24% compared to standard fumigation practices where fumigant injection is at 46 cm depth. Given the relatively wide range in emission-reduction percentages, a fumigant diffusion model was used to predict the percentage reduction in emissions by injecting at 61 cm, which yielded a 21% reduction in emissions. Significant reductions in emissions of 1,3-D and chloropicrin are possible by injecting soil fumigants deeper in soil. Published by Elsevier Ltd. C1 [Yates, S. R.; Ashworth, D. J.] USDA ARS, US Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. [Ashworth, D. J.] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Zheng, W.] Univ Illinois, Illinois Sustainable Technol Ctr, Champaign, IL 61820 USA. [Knuteson, J.] FluxExperts LLC, Indianapolis, IN USA. [van Wesenbeeck, I. J.] Dow Agrosci, 9330 Zionsville Rd, Indianapolis, IN 46268 USA. RP Yates, SR (reprint author), USDA ARS, US Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. EM scott.yates@ars.usda.gov FU California Air Resources Board [05-351] FX The use of trade, firm, or corporation names in this research article is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. The authors are grateful to Q. Zhang and J. Jobes for their assistance in preparing and conducting this experiment. Part of the research described was supported by California Air Resources Board (agreement #05-351). NR 48 TC 1 Z9 1 U1 4 U2 8 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD JUL PY 2016 VL 137 BP 135 EP 145 DI 10.1016/j.atmosenv.2016.04.042 PG 11 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DN8HQ UT WOS:000377320200013 ER PT J AU Dubey, MK Janowiak, J Mack, R Elder, P Hoover, K AF Dubey, Manoj K. Janowiak, John Mack, Ron Elder, Peggy Hoover, Kelli TI Comparative study of radio-frequency and microwave heating for phytosanitary treatment of wood SO EUROPEAN JOURNAL OF WOOD AND WOOD PRODUCTS LA English DT Article ID DIELECTRIC-PROPERTIES; RADIO-FREQUENCY; ISPM 15 AB Radio-frequency (RF) and microwave (MW) heating for phytosanitary treatment of green wood in compliance with international standards for phytosanitary measures no. 15 (ISPM-15) were evaluated and compared to assess treatment time, depth of electromagnetic wave (EMW) penetration and heating uniformity. White oak (Quercus alba) cants (48 cm long with cross-section dimensions ranging from 10 x 10 cm(2) to 25 x 25 cm(2)) were heated in a 19 MHz RF or 2.45 GHz MW laboratory oven using an equivalent heating power (3.4 kW). In each specimen, temperature was measured at different depths (distance from the upper face). Specimens were held in the treatment chamber for 2 min after the target temperature of 60 A degrees C was achieved through the profile of the specimen to ensure compliance with the ISPM-15 treatment schedule. Thermal image analyses of treated specimens as well as theoretical depth of penetration for dielectric energy were explored. Wood specimens were also heated using RF at high power (9-11 kW) and results were compared with RF heating at 3.4 kW. For wood with cross-section dimensions of 10 x 10 cm(2) to 15 x 15 cm(2), heating rates for RF and MW were relatively similar. However, above 15 x 15 cm(2), RF heating was more than 40 % faster with greater heating uniformity than MW. The theoretical values derived for depth of penetration and thermal image analyses indicate that RF (19 MHz) penetrates wood more uniformly and is better suited than MW (2.45 GHz) for bulk volume treatments of wood. C1 [Dubey, Manoj K.; Janowiak, John] Penn State Univ, Dept Agr & Biol Engn, Forest Resources Lab 101, University Pk, PA 16802 USA. [Mack, Ron; Elder, Peggy] USDA APHIS PPQ CPHST, Otis Lab, Buzzards Bay, MA 02542 USA. [Hoover, Kelli] Penn State Univ, Dept Entomol, 501 Ag Sci & Ind Bldg, University Pk, PA 16802 USA. RP Janowiak, J (reprint author), Penn State Univ, Dept Agr & Biol Engn, Forest Resources Lab 101, University Pk, PA 16802 USA. EM jjj2@psu.edu FU USDA-Methyl Bromide Transitions Program [2012-51102-20208] FX We thank Ben Wilson of PSC, Inc. for providing technical support for the RF oven, Glenn Blaker for his electrode modeling contributions, and Michael Powell for assistance with securing experimental materials. This study was supported from funding provided by the USDA-Methyl Bromide Transitions Program to Kelli Hoover and John Janowiak ( Grant 2012-51102-20208). NR 30 TC 0 Z9 0 U1 13 U2 17 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0018-3768 EI 1436-736X J9 EUR J WOOD WOOD PROD JI Eur. J. Wood Wood Prod. PD JUL PY 2016 VL 74 IS 4 BP 491 EP 500 DI 10.1007/s00107-016-1025-2 PG 10 WC Forestry; Materials Science, Paper & Wood SC Forestry; Materials Science GA DN8XP UT WOS:000377363500001 ER PT J AU Asadi, A Miller, M Moon, RJ Kalaitzidou, K AF Asadi, A. Miller, M. Moon, R. J. Kalaitzidou, K. TI Improving the interfacial and mechanical properties of short glass fiber/epoxy composites by coating the glass fibers with cellulose nanocrystals SO EXPRESS POLYMER LETTERS LA English DT Article DE polymer composites; nanomaterials; coatings; mechanical properties ID EPOXY COMPOSITES; POLYMER COMPOSITES; MATRIX ADHESION; NANOCOMPOSITES; BEHAVIOR; TENSILE AB In this study, the interfacial and mechanical properties of cellulose nanocrystals (CNC) coated glass fiber/epoxy composites were investigated as a function of the CNC content on the surface of glass fibers (GF). Chopped GF rovings were coated with CNC by immersing the GF in CNC (0-5 wt%) aqueous suspensions. Single fiber fragmentation (SFF) tests showed that the interfacial shear strength (IFSS) increased by similar to 69% in composites produced with CNC coated GF as compared to uncoated GF, suggesting an enhancement of stress transfer across the GF/matrix interface. The role of CNC coatings on the tensile, flexural, and thermo-mechanical properties of the CNC-coated GF/epoxy composites was investigated. Incorporation of 0.17 wt% CNC in the composite resulted in increases of similar to 10% in both elastic modulus and tensile strength, and 40 and 43 % in flexural modulus and strength respectively. In conclusion CNC coatings on GF alter the GF/matrix interface resulting in improvement of the mechanical performance of the corresponding composites. C1 [Asadi, A.; Miller, M.; Kalaitzidou, K.] Georgia Inst Technol, GW Woodruff Sch Mech Engn, Atlanta, GA 30332 USA. [Moon, R. J.] US Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. [Moon, R. J.; Kalaitzidou, K.] Georgia Inst Technol, Sch Mat Sci & Engn, Atlanta, GA 30332 USA. RP Kalaitzidou, K (reprint author), Georgia Inst Technol, GW Woodruff Sch Mech Engn, Atlanta, GA 30332 USA.; Kalaitzidou, K (reprint author), Georgia Inst Technol, Sch Mat Sci & Engn, Atlanta, GA 30332 USA. EM kyriaki.kalaitzidou@me.gatech.edu FU P3 Nano; U.S. Endowment for Forestry and Communities FX This work was supported by funding from P3 Nano and the U.S. Endowment for Forestry and Communities. The authors would like to thank Prof. Jon Colton for providing mechanical testing equipment. NR 36 TC 4 Z9 4 U1 11 U2 29 PU BUDAPEST UNIV TECHNOL & ECON PI BUDAPEST PA DEPT POLYMER ENG, MUEGYETEM RKP 3, BUDAPEST, H-1111, HUNGARY SN 1788-618X J9 EXPRESS POLYM LETT JI Express Polym. Lett. PD JUL PY 2016 VL 10 IS 7 BP 587 EP 597 DI 10.3144/expresspolymlett.2016.54 PG 11 WC Polymer Science SC Polymer Science GA DO0DU UT WOS:000377450400006 ER PT J AU Pan, LQ Lu, RF Zhu, QB Tu, K Cen, HY AF Pan, Leiqing Lu, Renfu Zhu, Qibing Tu, Kang Cen, Haiyan TI Predict Compositions and Mechanical Properties of Sugar Beet Using Hyperspectral Scattering SO FOOD AND BIOPROCESS TECHNOLOGY LA English DT Article DE Hyperspectral scattering; Sugar beet; Moisture content; Soluble solids content; Sucrose content; Mechanical properties ID NEAR-INFRARED SPECTROSCOPY; SOLUBLE SOLIDS CONTENT; APPLE FRUIT FIRMNESS; OPTICAL-PROPERTIES; SUCROSE CONTENT; NONDESTRUCTIVE DETERMINATION; REFLECTANCE SPECTROSCOPY; BIOLOGICAL-MATERIALS; SWNIR SPECTROSCOPY; QUALITY AB Sucrose, soluble solids, and moisture content and mechanical properties are important quality/property attributes of sugar beet. In this study, hyperspectral scattering images for the spectral region of 500-1000 nm were acquired, from which relative mean spectra were calculated. Prediction models were developed using partial least squares regression for both full spectra and selected wavelengths. The results showed that using relative mean spectra gave good predictions for the moisture, soluble solids, and sucrose content of beet slices with the correlations of 0.75-0.88 and the standard errors of prediction of 0.95-1.08 based on full-spectrum partial least squares regression (PLSR) models. PLSR models using wavelength selection with the uninformative variable elimination (UVE) method produced similar prediction accuracy. However, both modeling approaches gave poor predictions for the mechanical properties of beets with the correlation values of 0.46-0.63. The research demonstrated the potential of hyperspectral scattering imaging for measuring quality attributes of sugar beet. C1 [Pan, Leiqing; Tu, Kang] Nanjing Agr Univ, Coll Food Sci & Technol, 1 Weigang Rd, Nanjing 210095, Jiangsu, Peoples R China. [Lu, Renfu] Michigan State Univ, USDA ARS, Sugarbeet & Bean Res Unit, 524 S Shaw Lane,Room 224, E Lansing, MI 48824 USA. [Zhu, Qibing] Jiangnan Univ, Key Lab Adv Proc Control Light Ind, Minist Educ, 1800 Lihu Rd, Wuxi 214122, Peoples R China. [Cen, Haiyan] Michigan State Univ, Dept Food Sci & Human Nutr, E Lansing, MI 48824 USA. [Cen, Haiyan] Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310003, Zhejiang, Peoples R China. RP Pan, LQ (reprint author), Nanjing Agr Univ, Coll Food Sci & Technol, 1 Weigang Rd, Nanjing 210095, Jiangsu, Peoples R China. EM pan_leiqing@njau.edu.cn FU Chinese National Foundation of Natural Science [31101282, 61275155]; Special Fund for Agro-scientific Research in the Public Interest [201303088]; National Key Technology RD Program [2015BAD19B03] FX Authors Leiqing Pan, Qibing Zhu, and Kang Tu acknowledge the financial support from the Chinese National Foundation of Natural Science ( 31101282, 61275155), Special Fund for Agro-scientific Research in the Public Interest ( 201303088), and National Key Technology R&D Program ( 2015BAD19B03). NR 44 TC 1 Z9 1 U1 10 U2 14 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1935-5130 EI 1935-5149 J9 FOOD BIOPROCESS TECH JI Food Bioprocess Technol. PD JUL PY 2016 VL 9 IS 7 BP 1177 EP 1186 DI 10.1007/s11947-016-1710-5 PG 10 WC Food Science & Technology SC Food Science & Technology GA DN9WQ UT WOS:000377431800010 ER PT J AU Lee, CC Kibblewhite, RE Paavola, CD Orts, WJ Wagschal, K AF Lee, Charles C. Kibblewhite, Rena E. Paavola, Chad D. Orts, William J. Wagschal, Kurt TI Production of Glucaric Acid from Hemicellulose Substrate by Rosettasome Enzyme Assemblies SO MOLECULAR BIOTECHNOLOGY LA English DT Article DE Hemicellulose; Lignocellulases; Glucaric acid; Enzyme nanoassembly ID ALPHA-L-ARABINOFURANOSIDASES; PLANT-CELL WALLS; CELLULOSOME; PROTEIN; COMPLEX; DEGRADATION; HYDROLYSIS; MECHANISMS; CANCER; BIND AB Hemicellulose biomass is a complex polymer with many different chemical constituents that can be utilized as industrial feedstocks. These molecules can be released from the polymer and transformed into value-added chemicals through multistep enzymatic pathways. Some bacteria produce cellulosomes which are assemblies composed of lignocellulolytic enzymes tethered to a large protein scaffold. Rosettasomes are artificial engineered ring scaffolds designed to mimic the bacterial cellulosome. Both cellulosomes and rosettasomes have been shown to facilitate much higher rates of biomass hydrolysis compared to the same enzymes free in solution. We investigated whether tethering enzymes involved in both biomass hydrolysis and oxidative transformation to glucaric acid onto a rosettasome scaffold would result in an analogous production enhancement in a combined hydrolysis and bioconversion metabolic pathway. Three different enzymes were used to hydrolyze birchwood hemicellulose and convert the substituents to glucaric acid, a top-12 DOE value added chemical feedstock derived from biomass. It was demonstrated that colocalizing the three different enzymes to the synthetic scaffold resulted in up to 40 % higher levels of product compared to uncomplexed enzymes. C1 [Lee, Charles C.; Kibblewhite, Rena E.; Orts, William J.; Wagschal, Kurt] USDA ARS, Bioprod Res Unit, WRRC, 800 Buchanan St, Albany, CA 94710 USA. [Paavola, Chad D.] NASA, Ames Res Ctr, Moffett Field, CA 94035 USA. RP Lee, CC (reprint author), USDA ARS, Bioprod Res Unit, WRRC, 800 Buchanan St, Albany, CA 94710 USA. EM Charles.Lee@ars.usda.gov FU United States Department of Agriculture [CRIS 2030-41000-054-00]; National Institute of Food and Agriculture [2012-03998] FX We thank Bruce Mackey and Linda Whitehand for consultations on the statistical design of the experiments. This work was supported by the United States Department of Agriculture (CRIS 2030-41000-054-00) and National Institute of Food and Agriculture (Grant 2012-03998). The mention of firm names or trade products does not imply that they are endorsed or recommended by the US Department of Agriculture over other firms or similar products not mentioned. USDA is an equal opportunity provider and employer. NR 42 TC 1 Z9 1 U1 7 U2 12 PU HUMANA PRESS INC PI TOTOWA PA 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA SN 1073-6085 EI 1559-0305 J9 MOL BIOTECHNOL JI Mol. Biotechnol. PD JUL PY 2016 VL 58 IS 7 BP 489 EP 496 DI 10.1007/s12033-016-9945-y PG 8 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA DO0KL UT WOS:000377467700005 PM 27198564 ER PT J AU Wagschal, K Stoller, JR Chan, VJ Lee, CC Grigorescu, AA Jordan, DB AF Wagschal, Kurt Stoller, J. Rose Chan, Victor J. Lee, Charles C. Grigorescu, Arabela A. Jordan, Douglas B. TI Expression and Characterization of Hyperthermostable Exo-polygalacturonase TtGH28 from Thermotoga thermophilus SO MOLECULAR BIOTECHNOLOGY LA English DT Article DE GH28; Polygalacturonase; Hyperthermostable; Substrate inhibition; Product inhibition; Galacturonic acid; Pectin ID CRYSTAL-STRUCTURE; BIOCHEMICAL-CHARACTERIZATION; FUNCTIONAL-PROPERTIES; PATHOGEN INTERACTIONS; GLYCOSIDE HYDROLASE; INHIBITING PROTEINS; PECTATE LYASE; ACID; EXOPOLYGALACTURONASE; CLONING AB D-galacturonic acid is a potential platform chemical comprising the principal component of pectin in the citrus processing waste stream. Several enzyme activities are required for the enzymatic production of galacturonic acid from pectin, including exo- and endo-polygalacturonases. The gene TtGH28 encoding a putative GH28 polygalacturonase from Pseudothermotoga thermarum DSM 5069 (Theth_0397, NCBI# AEH50492.1) was synthesized, expressed in Escherichia coli, and characterized. Alignment of the amino acid sequence of gene product TtGH28 with other GH28 proteins whose structures and details of their catalytic mechanism have been elucidated shows that three catalytic Asp residues and several other key active site residues are strictly conserved. Purified TtGH28 was dimeric and hyperthermostable, with K (t) (0.5) = 86.3 A degrees C. Kinetic parameters for activity on digalacturonic acid, trigalacturonic acid, and polygalacturonic acid were obtained. No substrate inhibition was observed for polygalacturonate, while the K (si) values for the oligogalacturonides were in the low mM range, and K (i) for product galacturonic acid was in the low mu M range. Kinetic modeling of the progress of reaction showed that the enzyme is both fully exo- and fully non-processional. C1 [Wagschal, Kurt; Chan, Victor J.; Lee, Charles C.] USDA ARS, WRRC, 800 Buchanan St, Albany, CA 94710 USA. [Stoller, J. Rose; Jordan, Douglas B.] USDA ARS, NCAUR, 1815 N Univ St, Peoria, IL 61604 USA. [Grigorescu, Arabela A.] Northwestern Univ, Keck Biophys Facil, Evanston, IL 60201 USA. [Grigorescu, Arabela A.] Northwestern Univ, Dept Mol Biosci, Evanston, IL 60201 USA. RP Wagschal, K (reprint author), USDA ARS, WRRC, 800 Buchanan St, Albany, CA 94710 USA.; Jordan, DB (reprint author), USDA ARS, NCAUR, 1815 N Univ St, Peoria, IL 61604 USA. EM kurt.wagschal@ars.usda.gov; douglas.jordan@ars.usda.gov FU United States Department of Agriculture (USDA) [CRIS 5325-41000-049-00]; National Institute of Food and Agriculture [2012-03998]; USDA CRIS [3620-41000-118-00D]; DOE [DE-AC05-06OR23100]; NCI [CCSG P30 CA060553] FX This work was supported by the United States Department of Agriculture (USDA) CRIS 5325-41000-049-00 and the National Institute of Food and Agriculture grant 2012-03998 (V.C., C.C.L., K.W.), and USDA CRIS 3620-41000-118-00D (D.B.J. and J.R.S.). This research was also supported in part by an appointment to the Agricultural Research Service (ARS) Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and the USDA (J.R.S.). ORISE is managed by Oak Ridge Associated Universities (ORAU) under DOE contract number DE-AC05-06OR23100. All opinions expressed in this paper are the authors' and do not necessarily reflect the policies and views of USDA, ARS, DOE, or ORAU/ORISE. The Keck Biophysics Facility was supported in part by NCI grant CCSG P30 CA060553 awarded to the Robert H. Lurie Comprehensive Cancer Center at Northwestern University (A.A.G.). The mention of firm names or trade products does not imply that they are endorsed or recommended by the USDA over other firms or similar products not mentioned. USDA is an equal opportunity provider and employer. NR 62 TC 0 Z9 0 U1 8 U2 10 PU HUMANA PRESS INC PI TOTOWA PA 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA SN 1073-6085 EI 1559-0305 J9 MOL BIOTECHNOL JI Mol. Biotechnol. PD JUL PY 2016 VL 58 IS 7 BP 509 EP 519 DI 10.1007/s12033-016-9948-8 PG 11 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA DO0KL UT WOS:000377467700007 PM 27209035 ER PT J AU Jia, YL Zhou, E Lee, S Bianco, T AF Jia, Yulin Zhou, Erxun Lee, Seonghee Bianco, Tracy TI Coevolutionary Dynamics of Rice Blast Resistance Gene Pi-ta and Magnaporthe oryzae Avirulence Gene AVR-Pita 1 SO PHYTOPATHOLOGY LA English DT Review ID MEDIATED RESISTANCE; NATURAL VARIATION; WILD RELATIVES; DISEASE; GRISEA; LOCUS; EVOLUTION; SELECTION; MARKERS; GENOME AB The Pi-ta gene in rice is effective in preventing infections by Magnaporthe oryzae strains that contain the corresponding avirulence gene, AVR-Pita1. Diverse haplotypes of AVR-Pita1 have been identified from isolates of M. oryzae from rice production areas in the United States and worldwide. DNA sequencing and mapping studies have revealed that AVR-Pita1 is highly unstable, while expression analysis and quantitative resistance loci mapping of the Pi-ta locus revealed complex evolutionary mechanisms of Pi-ta-mediated resistance. Among these studies, several Pi-ta transcripts were identified, most of which are probably derived from alternative splicing and exon skipping, which could produce functional resistance proteins that support a new concept of coevolution of Pi-ta and AVR-Pita1. User-friendly DNA markers for Pi-ta have been developed to support marker-assisted selection, and development of new rice varieties with the Pi-ta markers. Genome-wide association studies revealed a link between Pi-ta-mediated resistance and yield components suggesting that rice has evolved a complicated defense mechanism against the blast fungus. In this review, we detail the current understanding of Pi-ta allelic variation, its linkage with rice productivity, AVR-Pita allelic variation, and the coevolution of Pi-ta and AVR-Pita in Oryza species and M. oryzae populations, respectively. We also review the genetic and molecular basis of Pi-ta and AVR-Pita interaction, and its value in marker-assisted selection and engineering resistance. C1 [Jia, Yulin; Bianco, Tracy] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. [Zhou, Erxun] South China Agr Univ, Dept Plant Pathol, Coll Agr, Guangzhou 510642, Guangdong, Peoples R China. [Lee, Seonghee] Univ Florida, Dept Hort Sci, Gulf Coast Res & Educ Ctr, Inst Food & Agr Sci, Wimauma, FL 33598 USA. RP Jia, YL (reprint author), USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. EM yulin.jia@ars.usda.gov NR 75 TC 1 Z9 1 U1 12 U2 26 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2016 VL 106 IS 7 BP 676 EP 683 DI 10.1094/PHYTO-02-16-0057-RVW PG 8 WC Plant Sciences SC Plant Sciences GA DO2WR UT WOS:000377642200001 PM 27070427 ER PT J AU Yu, XY Armstrong, CM Zhou, MG Duan, YP AF Yu, Xiaoyue Armstrong, Cheryl M. Zhou, Mingguo Duan, Yongping TI Bismerthiazol Inhibits Xanthomonas citri subsp citri Growth and Induces Differential Expression of Citrus Defense-Related Genes SO PHYTOPATHOLOGY LA English DT Article ID SYSTEMIC ACQUIRED-RESISTANCE; PHENYLALANINE AMMONIA-LYASE; ORYZAE PV. ORYZAE; SALICYLIC-ACID; CHALCONE SYNTHASE; PATHOGENIC BACTERIA; SIGNAL-TRANSDUCTION; ARABIDOPSIS; CANKER; BENZOTHIADIAZOLE AB Citrus canker, caused by Xanthomonas citri ssp. citri, is a serious disease that causes substantial economic losses to the citrus industry worldwide. The bactericide bismerthiazol has been used to control rice bacterial blight (X. oryzae pv. oryzae). In this paper, we demonstrate that bismerthiazol can effectively control citrus canker by both inhibiting the growth of X. citri ssp. citri and triggering the plant's host defense response through the expression of several pathogenesis-related genes (PR1, PR2, CHI, and RpRd1) and the nonexpresser of PR genes (NPR1, NPR2, and NPR3) in 'Duncan' grapefruit, especially at early treatment times. In addition, we found that bismerthiazol induced the expression of the marker genes CitCHS and CitCHI in the flavonoid pathway and the PAL1 (phenylalanine ammonia lyase 1) gene in the salicylic acid (SA) biosynthesis pathway at different time points. Moreover, bismerthiazol also induced the expression of the priming defense-associated gene AZI1. Taken together, these results indicate that the induction of the defense response in 'Duncan' grapefruit by bismerthiazol may involve the SA signaling pathway and the priming defense and that bismerthiazol may serve as an alternative to copper bactericides for the control of citrus canker. C1 [Yu, Xiaoyue; Zhou, Mingguo] Nanjing Agr Univ, Nanjing 210095, Jiangsu, Peoples R China. [Yu, Xiaoyue; Armstrong, Cheryl M.; Duan, Yongping] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP Zhou, MG (reprint author), Nanjing Agr Univ, Nanjing 210095, Jiangsu, Peoples R China.; Duan, YP (reprint author), USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. EM mgzhou@njau.edu.cn; YongPing.Duan@ars.usda.gov FU United States Department of Agriculture FX We would like to thank C. Latza and Y. Cui for their excellent technical assistance. Funding was provided by the United States Department of Agriculture. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 67 TC 0 Z9 0 U1 6 U2 14 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2016 VL 106 IS 7 BP 693 EP 701 DI 10.1094/PHYTO-12-15-0328-R PG 9 WC Plant Sciences SC Plant Sciences GA DO2WR UT WOS:000377642200003 PM 26882850 ER PT J AU Louzada, ES Vazquez, OE Braswell, WE Yanev, G Devanaboina, M Kunta, M AF Louzada, Eliezer S. Vazquez, Omar Ed Braswell, W. Evan Yanev, George Devanaboina, Madhavi Kunta, Madhurababu TI Distribution of 'Candidatus Liberibacter asiaticus' Above and Below Ground in Texas Citrus SO PHYTOPATHOLOGY LA English DT Article ID REAL-TIME PCR; QUANTITATIVE DISTRIBUTION; IDENTIFICATION; AMPLIFICATION; DISEASE; PLANTS; TREES AB Detection of 'Candidatus Liberibacter asiaticus' represents one of the most difficult, yet critical, steps of controlling Huanglongbing disease. Efficient detection relies on understanding the underlying distribution of bacteria within trees. To that end, we studied the distribution of 'Ca. L. asiaticus' in leaves of 'Rio Red' grapefruit trees and in roots of 'Valencia' sweet orange trees grafted onto sour orange rootstock. We performed two sets of leaf collection on grapefruit trees; the first a selective sampling targeting symptomatic leaves and their neighbors and the second a systematic collection disregarding symptomology. From uprooted orange trees, we exhaustively sampled fibrous roots. In this study, the presence of 'Ca. L. asiaticus' was detected in leaves using real-time polymerase chain reaction (PCR) targeting the 16S ribosomal gene and in roots using the rpIJ/rpIL ribosomal protein genes and was confirmed with conventional PCR and sequencing of the rpIJ/rpIL gene in both tissues. Among randomly collected leaves, 'Ca. L. asiaticus' was distributed in a patchy fashion. Detection of 'Ca. L. asiaticus' varied with leaf symptomology with symptomatic leaves showing the highest frequency (74%) followed by their neighboring asymptomatic leaves (30%), while randomly distributed asymptomatic leaves had the lowest frequency (20%). Among symptomatic leaves, we found statistically significant differences in mean number of bacterial cells with respect to both increasing distance of the leaf from the trunk and cardinal direction. The titer of 'Ca. L. asiaticus' cells was significantly greater on the north side of trees than on the south and west sides. Moreover, these directions showed different spatial distributions of 'Ca. L. asiaticus' with higher titers near the trunk on the south and west sides as opposed to further from the trunk on the north side. Similarly, we found spatial variation in 'Ca. L. asiaticus' distribution among root samples. 'Ca. L. asiaticus' was detected more frequently and bacterial abundances were higher among horizontally growing roots just under the soil surface (96%) than among deeper vertically growing roots (78%). Bacterial abundance declined slightly with distance from the trunk. These results point to paths of research that will likely prove useful to combating this devastating disease. C1 [Louzada, Eliezer S.; Vazquez, Omar Ed; Kunta, Madhurababu] Texas A&M Univ, Kingsville Citrus Ctr, 312 N Int Blvd, Weslaco, TX 78599 USA. [Braswell, W. Evan] USDA APHIS PPQ CPHST, Mission Lab, Edinburg, TX 78541 USA. [Yanev, George; Devanaboina, Madhavi] Univ Texas Rio Grande Valley, Dept Math, 1201 W Univ Dr, Edinburg, TX 78539 USA. RP Kunta, M (reprint author), Texas A&M Univ, Kingsville Citrus Ctr, 312 N Int Blvd, Weslaco, TX 78599 USA. EM madhura.kunta@tamuk.edu FU United States Department of Agriculture (USDA)-Animal and Plant Health Inspection Service-Center for Plant Health Science and Technology [13-8130-0305-CA]; Texas Agricultural and Mechanical University at Kingsville [13-8130-0305-CA]; NFSR at the MES of Bulgaria [DFNI-I02/17] FX This research was supported by a cooperative agreement 13-8130-0305-CA between the United States Department of Agriculture (USDA)-Animal and Plant Health Inspection Service-Center for Plant Health Science and Technology and Texas Agricultural and Mechanical University at Kingsville. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA, an equal opportunity employer. The forth author was partially supported by the NFSR at the MES of Bulgaria, grant number DFNI-I02/17, while on leave from the Institute of Mathematics and Informatics at the Bulgarian Academy of Sciences. NR 20 TC 0 Z9 0 U1 8 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2016 VL 106 IS 7 BP 702 EP 709 DI 10.1094/PHYTO-01-16-0004-R PG 8 WC Plant Sciences SC Plant Sciences GA DO2WR UT WOS:000377642200004 PM 27050571 ER PT J AU Newcomb, M Rouse, OMN Rouse, MN Szabo, LJ Johnson, J Gale, S Luster, DG Wanyera, R Macharia, G Bhavani, S Hodson, D Patpour, M Hovmoller, MS Fetch, TG Jin, Y AF Newcomb, Maria Olivera, Pablo D. Rouse, Matthew N. Szabo, Les J. Johnson, Jerry Gale, Sam Luster, Douglas G. Wanyera, Ruth Macharia, Godwin Bhavani, Sridhar Hodson, David Patpour, Mehran Hovmoller, Mogens S. Fetch, Thomas G., Jr. Jin, Yue TI Kenyan Isolates of Puccinia graminis f. sp tritici from 2008 to 2014: Virulence to SrTmp in the Ug99 Race Group and Implications for Breeding Programs SO PHYTOPATHOLOGY LA English DT Article ID STEM RUST RESISTANCE; FOOD SECURITY; WHEAT; GENE; TTKS; IDENTIFICATION; EMERGENCE; PATHOGEN; SPREAD; THREAT AB Frequent emergence of new variants in the Puccinia graminis f sp. tritici Ug99 race group in Kenya has made pathogen survey a priority. We analyzed 140 isolates from 78 P graminis f. sp. tritici samples collected in Kenya between 2008 and 2014 and identified six races, including three not detected prior to 2013. Genotypic analysis of 20 isolates from 2013 and 2014 collections showed that the new races TTHST, TTKTK, and TTKTT belong to the Ug99 race group. International advanced breeding lines were evaluated against an isolate of TTKTT (Sr31, Sr24, and SrTnzp virulence) at the seedling stage. From 169 advanced lines from Kenya, 23% of lines with resistance to races TTKSK and TTKST were susceptible to TTKTT and, from two North American regional nurseries, 44 and 91% of resistant lines were susceptible. Three lines with combined resistance genes were developed to facilitate pathogen monitoring and race identification. These results indicate the increasing virulence and variability in the Kenyan P. graminis f. sp. tritici population and reveal vulnerabilities of elite gennplasm to new races. C1 [Newcomb, Maria; Olivera, Pablo D.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Rouse, Matthew N.; Szabo, Les J.; Johnson, Jerry; Gale, Sam; Jin, Yue] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Luster, Douglas G.] USDA ARS, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA. [Wanyera, Ruth; Macharia, Godwin] Kenya Agr & Livestock Res Org, Njoro, Kenya. [Bhavani, Sridhar] Int Maize & Wheat Improvement Ctr CIMMYT Kenya, Nairobi, Kenya. [Hodson, David] CIMMYT Ethiopia, Addis Ababa, Ethiopia. [Patpour, Mehran; Hovmoller, Mogens S.] Aarhus Univ, Dept Agroecol, DK-4200 Slagelse, Denmark. [Fetch, Thomas G., Jr.] Agr & Agri Food Canada, Brandon, MB, Canada. RP Jin, Y (reprint author), Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. EM Yue.Jin@ARS.USDA.GOV RI Rouse, Matthew/G-5474-2011 FU Durable Rust Resistance in Wheat project; Bill and Melinda Gates Foundation; United Kingdom Department for International Development; USDA-ARS National Plant Disease Recovery Plan FX This research received support from the Durable Rust Resistance in Wheat project administrated by Cornell University and funded by the Bill and Melinda Gates Foundation and the United Kingdom Department for International Development, and from the USDA-ARS National Plant Disease Recovery Plan. We thank S. Stoxen, L. Wanschura, M. Carter, and the KALRO pathology team of H. Wanga, P. Kinyanjui, F. Muchika, E. Githinji, L. Wolukao, V. M. Onyango, and E. O. Okello for their technical contributions. Mention of trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the USDA, and does not imply its approval to the exclusion of other products and vendors that might also be suitable. NR 30 TC 3 Z9 3 U1 2 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2016 VL 106 IS 7 BP 729 EP 736 DI 10.1094/PHYTO-12-15-0337-R PG 8 WC Plant Sciences SC Plant Sciences GA DO2WR UT WOS:000377642200007 PM 27019064 ER PT J AU Johnson, RM Grisham, MP Warnke, KZ Maggio, JR AF Johnson, Richard M. Grisham, Michael P. Warnke, Kathryn Z. Maggio, Jeri R. TI Relationship of Soil Properties and Sugarcane Yields to Red Stripe in Louisiana SO PHYTOPATHOLOGY LA English DT Article ID RUST SEVERITY AB Symptoms of red stripe disease caused by Acidovorax avenae subsp. avenae in Louisiana between 1985 and 2010 were limited to the leaf stripe form, which caused no apparent yield loss. During 2010, the more severe top rot form was observed, and a study was initiated to investigate the distribution of red stripe in the field and determine its effects on cane and sugar yields. Soil properties data, red stripe incidence, and sugarcane yields were all highly variable and were not randomly distributed in the field. Combined harvest data showed a negative correlation between yield components and red stripe incidence, with the strongest relationship between sucrose per metric ton and disease incidence. Red stripe incidence was positively correlated with several soil properties, including phosphorus, potassium, zinc, and calcium. Red stripe incidence also was found to increase with increasing nitrogen rate, with the greatest effects in heavy soils. Results also indicated that using red-stripe-infected cane as a seed source can significantly decrease shoot emergence, stalk population, and subsequent cane and sugar yields. These combined data suggest that red stripe disease can exhibit a highly variable rate of infection in commercial sugarcane fields and may also significantly decrease sugar yields. C1 [Johnson, Richard M.; Grisham, Michael P.; Warnke, Kathryn Z.; Maggio, Jeri R.] USDA ARS, Sugarcane Res Unit, 5883 USDA Rd, Houma, LA 70360 USA. RP Johnson, RM (reprint author), USDA ARS, Sugarcane Res Unit, 5883 USDA Rd, Houma, LA 70360 USA. EM richard.johnson@ars.usda.gov FU United States Department of Agriculture-Agricultural Research Service FX This research was supported by funds from the United States Department of Agriculture-Agricultural Research Service. We thank D. Jones, B. King, and J. Rome for assistance in soil sampling and R. Richard for assistance in red stripe monitoring. Mention of a trademark, proprietary product or vendor does not constitute a guarantee or warranty of the product by the United States Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that may also be suitable. NR 18 TC 0 Z9 0 U1 4 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2016 VL 106 IS 7 BP 737 EP 744 DI 10.1094/PHYTO-09-15-0218-R PG 8 WC Plant Sciences SC Plant Sciences GA DO2WR UT WOS:000377642200008 PM 27003508 ER PT J AU Olukolu, BA Tracy, WF Wisser, R De Vries, B Balint-Kurti, PJ AF Olukolu, Bode A. Tracy, William F. Wisser, Randall De Vries, Brian Balint-Kurti, Peter J. TI A Genome-Wide Association Study for Partial Resistance to Maize Common Rust SO PHYTOPATHOLOGY LA English DT Article DE association analysis; disease resistance; linkage disequilibrium; QTL ID QUANTITATIVE TRAIT LOCI; SWEET CORN; LEAF RUST; LINKAGE DISEQUILIBRIUM; GENETIC ARCHITECTURE; DISEASE RESISTANCE; KERNEL COMPOSITION; GERMINATED BARLEY; PUCCINIA-SORGHI; PROTEIN AB Quantitative resistance to maize common rust (causal agent Puccinia sorghi) was assessed in an association mapping population of 274 diverse inbred lines. Resistance to common rust was found to be moderately correlated with resistance to three other diseases and with the severity of the hypersensitive defense response previously assessed in the same population. Using a mixed linear model accounting for the confounding effects of population structure and flowering time, genome-wide association tests were performed based at 246,497 single-nucleotide polymorphism loci. Three loci associated with maize common rust resistance were identified. Candidate genes at each locus had predicted roles, mainly in cell wall modification. Other candidate genes included a resistance gene and a gene with a predicted role in regulating accumulation of reactive oxygen species. C1 [Olukolu, Bode A.; Balint-Kurti, Peter J.] N Carolina State Univ, Dept Plant Pathol, Box 7616, Raleigh, NC 27695 USA. [Olukolu, Bode A.] N Carolina State Univ, Dept Hort, Raleigh, NC 27695 USA. [Tracy, William F.; De Vries, Brian] Univ Wisconsin, Dept Agron, 1575 Linden Dr, Madison, WI 53706 USA. [Wisser, Randall] Univ Delaware, Dept Plant & Soil Sci, Newark, DE 19716 USA. [Balint-Kurti, Peter J.] USDA ARS, Plant Sci Res Unit, Raleigh, NC 27695 USA. RP Olukolu, BA; Balint-Kurti, PJ (reprint author), N Carolina State Univ, Dept Plant Pathol, Box 7616, Raleigh, NC 27695 USA.; Olukolu, BA (reprint author), N Carolina State Univ, Dept Hort, Raleigh, NC 27695 USA.; Balint-Kurti, PJ (reprint author), USDA ARS, Plant Sci Res Unit, Raleigh, NC 27695 USA. EM baolukol@ncsu.edu; peter.balint-kurti@ars.usda.gov FU United States Department of Agriculture Agricultural Research Service; National Science Foundation Plant Genome research program [0822495] FX This work was funded by the United States Department of Agriculture Agricultural Research Service and by the National Science Foundation Plant Genome research program grant number 0822495. We thank M. Goodman and the Maize Genetics Cooperation Stock Center for donating seed; E. Buckler, P. Bradbury, J. Glaubitz, and the Maize Diversity project team for access to the GBS genotype dataset; and J. Holland for helpful discussions. NR 59 TC 3 Z9 3 U1 5 U2 12 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2016 VL 106 IS 7 BP 745 EP 751 DI 10.1094/PHYTO-11-15-0305-R PG 7 WC Plant Sciences SC Plant Sciences GA DO2WR UT WOS:000377642200009 PM 27003507 ER PT J AU Rivera, Y Salgado-Salazar, C Gulya, TJ Crouch, JA AF Rivera, Yazmin Salgado-Salazar, Catalina Gulya, Thomas J. Crouch, Jo Anne TI Newly Emerged Populations of Plasmopara halstedii Infecting Rudbeckia Exhibit Unique Genotypic Profiles and Are Distinct from Sunflower-Infecting Strains SO PHYTOPATHOLOGY LA English DT Article DE microsatellites ID SINGLE NUCLEOTIDE POLYMORPHISMS; DOWNY MILDEW PATHOGENS; SEQUENCE DATA; 1ST REPORT; PHYLOGENETIC-RELATIONSHIPS; MULTIPLE INTRODUCTIONS; MAXIMUM-LIKELIHOOD; GENETIC-ANALYSIS; CAUSAL AGENT; INFERENCE AB The oomycete Plasmopara halstedii emerged at the onset of the 21st century as a destructive new pathogen causing downy mildew disease of ornamental Rudbeckia fulgida (rudbeckia) in the United States. The pathogen is also a significant global problem of sunflower (Helianthus annuus) and is widely regarded as the cause of downy mildew affecting 35 Asteraceae genera. To determine whether rudbeckia and sunflower downy mildew are caused by the same genotypes, population genetic and phylogenetic analyses were performed. A draft genome assembly of a P. halstedii isolate from sunflower was generated and used to design 15 polymorphic simple sequence repeat (SSR) markers. SSRs and two sequenced phylogenetic markers measured differentiation between 232 P. halstedii samples collected from 1883 to 2014. Samples clustered into two main groups, corresponding to host origin. Sunflower-derived samples separated into eight admixed subclusters, and rudbeckia-derived samples further separated into three subclusters. Pre-epidemic rudbeckia samples clustered separately from modern strains. Despite the observed genetic distinction based on host origin, P. halstedii from rudbeckia could infect sunflower, and exhibited the virulence phenotype of race 734. These data indicate that the newly emergent pathogen populations infecting commercial rudbeckia are a different species from sunflower-infecting strains, notwithstanding cross-infectivity, and genetically distinct from pre-epidemic populations infecting native rudbeckia hosts. C1 [Rivera, Yazmin; Salgado-Salazar, Catalina; Crouch, Jo Anne] USDA ARS, Systemat Mycol & Microbiol Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. [Rivera, Yazmin; Salgado-Salazar, Catalina] Rutgers State Univ, Dept Plant Biol & Pathol, 59 Dudley Rd, New Brunswick, NJ 08906 USA. [Gulya, Thomas J.] USDA ARS, Sunflower & Plant Biol Res Unit, 1605 Albrecht Blvd North, Fargo, ND 58102 USA. RP Crouch, JA (reprint author), USDA ARS, Systemat Mycol & Microbiol Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM joanne.crouch@ars.usda.gov OI Crouch, Jo Anne/0000-0001-6886-8090; Salgado Salazar, Catalina/0000-0002-4156-692X FU USDA-APHIS-PPQ Farm Bill grant; USDA-ARS project [8042-22000-279] FX We thank J. Byrne, D. Clement, T. Creswell, M. Daughtrey, S. Jensen, K. Snover-Clift, R. Leatherwood, C. Misar, R. Peterman, L. Pundt, K. Rane, M. Romberg, and A. Windham for providing fresh samples of P. halstedii on sunflower and rudbeckia; N. Saied, C. Misar, and M. Gilley for technical support; S. Sheffer and M. Lewis for the use of the ABI 3730x1 instrument; and the U.S. National Fungus Collection for allowing access to herbarium specimens. We are particularly indebted to A. Gould for her continued partnership and support, and for her ongoing mentorship of Y. Rivera and C. Salgado-Salazar. This research was funded by a 2013 USDA-APHIS-PPQ Farm Bill grant to JAC and USDA-ARS project 8042-22000-279; Y. Rivera and C. Salgado-Salazar were supported by 2013-2015 USDA-APHIS-PPQ Farm Bill grants to J. A. Crouch. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 72 TC 1 Z9 1 U1 4 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2016 VL 106 IS 7 BP 752 EP 761 DI 10.1094/PHYTO-12-15-0335-R PG 10 WC Plant Sciences SC Plant Sciences GA DO2WR UT WOS:000377642200010 PM 27003506 ER PT J AU Madeira, PT Hill, MP Dray, FA Coetzee, JA Paterson, ID Tipping, PW AF Madeira, P. T. Hill, M. P. Dray, F. A., Jr. Coetzee, J. A. Paterson, I. D. Tipping, P. W. TI Molecular identification of Azolla invasions in Africa: The Azolla specialist, Stenopelmus rufinasus proves to be an excellent taxonomist SO SOUTH AFRICAN JOURNAL OF BOTANY LA English DT Article DE Azolla; Biological control; Exotic; Invasive; Molecular taxonomy ID SUCCESSFUL BIOLOGICAL-CONTROL; FILICULOIDES LAM. AZOLLACEAE; 3 NONCODING REGIONS; SOUTH-AFRICA; PHYLOGENETIC-RELATIONSHIPS; LIFE-HISTORY; FERN; AMPLIFICATION; SALVINIACEAE; TEMPERATURE AB Biological control of Azolla filiculoides in South Africa with the Azolla specialist Stenopelmus rufinasus has been highly successful. However, field surveys showed that the agent utilized another Azolla species, thought to be the native Azolla pinnata subsp. africana, which contradicted host specificity trials. It is notoriously difficult to determine Azolla species based on morphology so genetic analyses were required to confirm the identity of the Azolla used by the agent. Extensive sampling was conducted and samples were sequenced at the trnL-trnF and trnG-trnR chloroplastic regions and the nuclear ITS1 region. Current literature reported A. filiculoides as the only Section Azolla species in southern Africa but 24 samples were identified as Azolla cristata, an introduced species within Section Azolla thatwas not used during host specificity trials. A. pinnata subsp. africana was only located at one site in southern Africa, while the alien A. pinnata subsp. asiatica was located at three. What was thought to be A. pinnata subsp. africana was in fact A. cristata, a closer relative of A. filiculoides and a suitable host according to specificity trials. This study confirms that S. rufinasus is a proficient Azolla taxonomist but also supports the use of molecular techniques for resolving taxonomic conundrums. (C) 2016 SAAB. Published by Elsevier B.V. All rights reserved. C1 [Madeira, P. T.; Dray, F. A., Jr.; Tipping, P. W.] ARS, USDA, Invas Plant Res Lab, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. [Hill, M. P.; Coetzee, J. A.; Paterson, I. D.] Rhodes Univ, Dept Zool & Entomol, ZA-6140 Grahamstown, South Africa. RP Hill, MP (reprint author), Rhodes Univ, Dept Zool & Entomol, ZA-6140 Grahamstown, South Africa. EM m.p.hill@ru.ac.za NR 49 TC 1 Z9 1 U1 1 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0254-6299 EI 1727-9321 J9 S AFR J BOT JI S. Afr. J. Bot. PD JUL PY 2016 VL 105 BP 299 EP 305 DI 10.1016/j.sajb.2016.03.007 PG 7 WC Plant Sciences SC Plant Sciences GA DN8AZ UT WOS:000377302600034 ER PT J AU Velazquez-Salinas, L Risatti, GR Holinka, LG O'Donnell, V Carlson, J Alfano, M Rodriguez, LL Carrillo, C Gladue, DP Borca, MV AF Velazquez-Salinas, Lauro Risatti, Guillermo R. Holinka, Lauren G. O'Donnell, Vivian Carlson, Jolene Alfano, Marialexia Rodriguez, Luis. L. Carrillo, Consuelo Gladue, Douglas P. Borca, Manuel V. TI Recoding structural glycoprotein E2 in classical swine fever virus (CSFV) produces complete virus attenuation in swine and protects infected animals against disease SO VIROLOGY LA English DT Article DE Codon usage; Codon de-optimization; Protection; Vaccine development; Classical swine fever virus ID CODON USAGE BIAS; HOG-CHOLERA VIRUS; STRAIN BRESCIA; DINUCLEOTIDE FREQUENCIES; VIRULENCE DETERMINANT; VIRAL VIRULENCE; GENE-EXPRESSION; RNA VIRUSES; PAIR BIAS; DEOPTIMIZATION AB Controlling classical swine fever (CSF) mainly involves vaccination with live attenuated vaccines (LAV). Experimental CSFV LAVs has been lately developed through reverse genetics using several different approaches. Here we present that codon de-optimization in the major CSFV structural glycoprotein E2 coding region, causes virus attenuation in swine. Four different mutated constructs (pCSFm1-pCSFm4) were designed using various mutational approaches based on the genetic background of the highly virulent strain Brescia (BICv). Three of these constructs produced infectious viruses (CSFm2v, CSFm3v, and CSFm4v). Animals infected with CSFm2v presented a reduced and extended viremia but did not display any CSF-related clinical signs. Animals that were infected with CSFm2v were protected against challenge with virulent parental BICv. This is the first report describing the development of an attenuated CSFV experimental vaccine by codon usage de-optimization, and one of the few examples of virus attenuation using this methodology that is assessed in a natural host. Published by Elsevier Inc. C1 [Velazquez-Salinas, Lauro; Holinka, Lauren G.; O'Donnell, Vivian; Carlson, Jolene; Alfano, Marialexia; Rodriguez, Luis. L.; Gladue, Douglas P.; Borca, Manuel V.] ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY 11944 USA. [Risatti, Guillermo R.; O'Donnell, Vivian] Univ Connecticut, Dept Pathobiol & Vet Sci, Storrs, CT 06269 USA. [Carrillo, Consuelo] DHS, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. [Carlson, Jolene] Kansas State Univ, Biosecur Res Inst, Coll Vet Med, Manhattan, KS 66506 USA. [Carlson, Jolene] Kansas State Univ, Dept Diagnost Med & Pathobiol, Coll Vet Med, Manhattan, KS 66506 USA. RP Borca, MV (reprint author), ARS, Plum Isl Anim Dis Ctr, USDA, NAA, POB 848, Greenport, NY 11944 USA. EM lauro.velazquez@ars.usda.gov; guillermo.risatti@uconn.edu; lauren.holinka@ars.usda.gov; vivian.odonnell@ars.usda.gov; jolene.carlson@ars.usda.gov; marialexia.alfano@ars.usda.gov; luis.rodriguez@ars.usda.gov; consuelo.carrillo@aphis.usda.gov; douglas.gladue@ars.usda.gov; manuel.borca@ars.usda.gov OI Gladue, Douglas/0000-0002-7894-0233; Borca, Manuel/0000-0002-0888-1178 FU State of Kansas National Bio and Agro-Defense Facility Fund (NBAF); Institute for Infectious Animal Diseases (IIAD) Career Development Program FX We thank the Plum Island Animal Disease Center Animal Care Unit staff for excellent technical assistance. We particularly like to thank Melanie Prarat for editing the manuscript. We also thank Dr Peter Simmonds for allowing the use of SSE program used for CPB analysis and dinucleotide calculation (Simmonds, 2012). This work was partially supported by the State of Kansas National Bio and Agro-Defense Facility Fund (NBAF) and Institute for Infectious Animal Diseases (IIAD) Career Development Program. NR 52 TC 0 Z9 0 U1 2 U2 10 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD JUL PY 2016 VL 494 BP 178 EP 189 DI 10.1016/j.virol.2016.04.007 PG 12 WC Virology SC Virology GA DN8IA UT WOS:000377321200017 PM 27110709 ER PT J AU Bertran, K Swayne, DE Pantin-Jackwood, MJ Kapczynski, DR Spackman, E Suarez, DL AF Bertran, Kateri Swayne, David E. Pantin-Jackwood, Mary J. Kapczynski, Darrell R. Spackman, Erica Suarez, David L. TI Lack of chicken adaptation of newly emergent Eurasian H5N8 and reassortant H5N2 high pathogenicity avian influenza viruses in the US is consistent with restricted poultry outbreaks in the Pacific flyway during 2014-2015 SO VIROLOGY LA English DT Article DE Avian influenza; Highly pathogenic avian influenza; Glade 2.3.4.4; Infectivity; Pathobiology; Poultry; Wild waterfowl; Transmission ID SOUTH-KOREA; WILD DUCK; BIRDS AB In 2014-2015, the U.S. experienced an unprecedented outbreak of Eurasian Glade 2.3.4.4 H5 highly pathogenic avian influenza (HPAI) virus, initially affecting mainly wild birds and few backyard and commercial poultry premises. To better model the outbreak, the pathogenesis and transmission dynamics of representative Eurasian H5N8 and reassortant H5N2 Glade 2.3.4.4 HPAI viruses detected early in the North American outbreak were investigated in chickens. High mean chicken infectious doses and lack of seroconversion in survivors indicated the viruses were poorly chicken adapted. Pathobiological features were consistent with HPAI virus infection, although the delayed appearance of lesions, longer mean death times, and reduced replication in endothelial cells differed from features of most other Eurasian H5N1 HPAI viruses. Although these initial U.S. H5 HPAI viruses had reduced adaptation and transmissibility in chickens, multi-generational passage in poultry could generate poultry adapted viruses with higher infectivity and transmissibility. (C) 2016 Published by Elsevier Inc. C1 [Bertran, Kateri; Swayne, David E.; Pantin-Jackwood, Mary J.; Kapczynski, Darrell R.; Spackman, Erica; Suarez, David L.] ARS, Exot & Emerging Avian Viral Dis Res Unit, SE Poultry Res Lab, US Natl Poultry Res Ctr,USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. RP Suarez, DL (reprint author), ARS, Exot & Emerging Avian Viral Dis Res Unit, SE Poultry Res Lab, US Natl Poultry Res Ctr,USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. EM kateri.bertran@ars.usda.gov; david.swayne@ars.usda.gov; mary.pantin-jackwood@ars.usda.gov; darrell.kapczynski@ars.usda.gov; erica.spackman@ars.usda.gov; david.suarez@ars.usda.gov FU USDA/ARS CRIS Project [6040-320000-63]; CRIP (Center of Research in Influenza Pathogenesis) an NIAID - Center of Excellence in Influenza Research and Surveillance (CEIRS) [HHSN272201400008C] FX This work was funded by USDA/ARS CRIS Project 6040-320000-63 and by CRIP (Center of Research in Influenza Pathogenesis) an NIAID funded Center of Excellence in Influenza Research and Surveillance (CEIRS, contract HHSN272201400008C). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the USDA or NIH. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The authors gratefully acknowledge Suzanne DeBlois, Kira Moresco, Marisela Rodriguez, Charles Balzli, Diane Smith, Megan Christian, and Mar Costa-Hurtado for their excellent technical assistance. NR 24 TC 5 Z9 5 U1 2 U2 6 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD JUL PY 2016 VL 494 BP 190 EP 197 DI 10.1016/j.virol.2016.04.019 PG 8 WC Virology SC Virology GA DN8IA UT WOS:000377321200018 PM 27110710 ER PT J AU Wraight, SP Filotas, MJ Sanderson, JP AF Wraight, S. P. Filotas, M. J. Sanderson, J. P. TI Comparative efficacy of emulsifiable-oil, wettable-powder, and unformulated-powder preparations of Beauveria bassiana against the melon aphid Aphis gossypii SO BIOCONTROL SCIENCE AND TECHNOLOGY LA English DT Article DE Beauveria bassiana; Aphis gossypii; mycopesticide; bioassay; aqueous formulation; oil formulation ID METARHIZIUM-ANISOPLIAE; ENTOMOPATHOGENIC FUNGI; DIFFERENT FORMULATIONS; SCHISTOCERCA-GREGARIA; TRIATOMA-INFESTANS; MYZUS-PERSICAE; FLAVOVIRIDE; CONIDIA; PERSISTENCE; MYCOINSECTICIDES AB Aphidicidal efficacy of two formulations of Beauveria bassiana strain GHA conidia, an emulsifiable paraffinic oil dispersion (OD) and a clay-based wettable powder (WP), were compared to unformulated conidia (technical material, TC) in laboratory bioassays with adult melon/cotton aphids. For the initial 24 h post-treatment, aphids were incubated under differing humidity conditions: high (100%) versus 'low' (75 +/- 3%) relative humidity (RH), and over the subsequent 6 days, all aphids were incubated under the low RH conditions. Mortality from the OD and TC treatments did not differ significantly under any test conditions, and ANOVA revealed no synergism of B. bassiana activity by the OD carrier ingredients. In contrast, formulation as a WP had a small, but significant, negative impact on B. bassiana efficacy. Mean LC50 across formulations was nearly 6.5-fold higher (3457 vs. 539 conidia/mm(2)) at low vs. high RH. The OD formulation was significantly more effective than the WP, increasing mortality a maximum of 27 percentage points. There were no significant effects of formulation on probit regression slopes. The equivalent slopes and small differences in efficacy of the OD formulation vs. TC preparation support a hypothesis that oils function primarily as spray stickers and spreaders, increasing the efficiency of spray applications. C1 [Wraight, S. P.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Filotas, M. J.] OMAFRA, Simcoe Off, Simcoe, ON, Canada. [Sanderson, J. P.] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. RP Wraight, SP (reprint author), USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM steve.wraight@ars.usda.gov FU USDA, ARS Biological Integrated Pest Management Research Unit [58-1097-4-447]; Cornell University Department of Entomology, Ithaca, New York [SCA] [58-1097-4-447]; USDA, ARS Floriculture and Nursery Research Initiative FX This research was supported in part through a Specific Cooperative Agreement between the USDA, ARS Biological Integrated Pest Management Research Unit and the Cornell University Department of Entomology, Ithaca, New York [SCA #58-1097-4-447] with funding through the USDA, ARS Floriculture and Nursery Research Initiative. NR 51 TC 1 Z9 1 U1 8 U2 24 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND SN 0958-3157 EI 1360-0478 J9 BIOCONTROL SCI TECHN JI Biocontrol Sci. Technol. PD JUL PY 2016 VL 26 IS 7 BP 894 EP 914 DI 10.1080/09583157.2016.1157851 PG 21 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DN4GO UT WOS:000377022100002 ER PT J AU Divilov, K Walker, DR AF Divilov, Konstantin Walker, David R. TI Reaction of Diaporthe longicolla to a strain of Sarocladium kiliense SO BIOCONTROL SCIENCE AND TECHNOLOGY LA English DT Article DE Phomopsis; Acremonium; soybean; biological control; parasitism; mycoparasite ID SOYBEAN SEED-GERMINATION; ACREMONIUM-STRICTUM BCP; CERCOSPORA-KIKUCHII; PHOMOPSIS SP; BORNE FUNGI; STEM BLIGHT; PUERTO-RICO; PHASEOLORUM; INFECTION; DECAY AB Phomopsis seed decay of soybean [Glycine max (L.) Merr.] is a seedborne fungal disease caused by Diaporthe longicolla that causes yield losses and reduced seed quality. Biocontrol of this pathogen by a strain of Sarocladium kiliense isolated from a culture of D. longicolla was investigated in vitro and in vivo. A zone of inhibition formed between the two fungi in vitro, but was poorly sustained, and inhibition of conidial germination of D. longicolla by a culture filtrate of S. kiliense was not significant, though D. longicolla mycelial growth was inhibited by a 25% culture filtrate. Co-inoculation of both fungi failed to reduce seed rot or increase seed germination in greenhouse and growth chamber experiments, respectively. Co-inoculation of both fungi also failed to reduce pycnidial development in colonies of D. longicolla growing on leaf pieces, but soaking of the leaf pieces with S. kiliense conidia for one or three days prior to inoculation with D. longicolla eliminated pycnidial development completely. Although it was not possible to reproduce the parasitism of S. kiliense on D. longicolla under the experimental and environmental conditions used, the potential to use S. kiliense as a protectant biocontrol for soybean fungal pathogens should be further investigated. C1 [Divilov, Konstantin; Walker, David R.] Univ Illinois, Dept Crop Sci, Urbana, IL USA. [Walker, David R.] USDA ARS, Soybean Maize Germplasm Pathol & Genet Res Unit, Urbana, IL USA. RP Walker, DR (reprint author), Univ Illinois, Dept Crop Sci, Urbana, IL USA.; Walker, DR (reprint author), USDA ARS, Soybean Maize Germplasm Pathol & Genet Res Unit, Urbana, IL USA. EM david.walker@ars.usda.gov FU USDA Agricultural Research Service; United Soybean Board: 'Screening germplasm and breeding for resistance to Phomopsis seed decay of soybean' [2261] FX This work was supported by USDA Agricultural Research Service and United Soybean Board: [grant number 2261] 'Screening germplasm and breeding for resistance to Phomopsis seed decay of soybean.' NR 49 TC 0 Z9 0 U1 4 U2 5 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND SN 0958-3157 EI 1360-0478 J9 BIOCONTROL SCI TECHN JI Biocontrol Sci. Technol. PD JUL PY 2016 VL 26 IS 7 BP 938 EP 950 DI 10.1080/09583157.2016.1169509 PG 13 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DN4GO UT WOS:000377022100005 ER PT J AU Hogg, BN Smith, L Moran, PJ Daane, KM AF Hogg, Brian N. Smith, Lincoln Moran, Patrick J. Daane, Kent M. TI Post-establishment assessment of host plant specificity of Arytainilla spartiophila (Hemiptera: Psyllidae), an adventive biological control agent of Scotch broom, Cytisus scoparius SO BIOCONTROL SCIENCE AND TECHNOLOGY LA English DT Article DE Host range; host specificity; Psyllidae; Scotch broom; weed control ID WEED BIOCONTROL; NEW-ZEALAND; NONTARGET PLANTS; NATIVE PLANTS; L WIMMER; RISK; RANGE; ATTACK; FIELD; AUSTRALIA AB Scotch broom, Cytisus scoparius (Fabaceae), is a shrub native to Europe that is invasive in the USA, New Zealand and Australia. The psyllid Arytainilla spartiophila has been purposely introduced to Australia and New Zealand as a biological control agent of C. scoparius, but is an accidental introduction to California. Lupines upinus spp.) are the closest native taxon to Cytisus in North America, and are therefore considered to be at the highest risk for non-target damage. However, because no lupines are native to Australia or New Zealand, only one imported forage species was evaluated during prior host specificity testing. We conducted a laboratory nymphal transfer experiment, a field choice experiment and a field survey to assess risk to three lupine species (Lupinus albifrons, Lupinus bicolor and Lupinus formosus). In the laboratory, 20% of third-lnstar nymphs were able to develop to adulthood on L. formosus but not on the other lupine species, while 40% completed development on C. scoparius. In the field experiment, potted lupine and C. scoparius plants were, placed beside large infested C. scoparius plants; oviposition occurred on all the potted C. scoparius plants, but on none of the lupines. In the field survey, no A. spartiophila eggs or nymphs were found on naturally occurring lupines growing adjacent to infested C. scoparius. The results indicate that A. spartiophiia is not likely to damage or eproduce on lupines in the field. This study provides an example of how field studies can help clarify the host specificity of biological control agents. C1 [Hogg, Brian N.; Daane, Kent M.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, 137 Mulford Hall, Berkeley, CA 94720 USA. [Hogg, Brian N.; Smith, Lincoln; Moran, Patrick J.] USDA ARS, Exot & Invas Weeds Res Unit, Albany, CA USA. RP Hogg, BN (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, 137 Mulford Hall, Berkeley, CA 94720 USA. EM brian.hogg@ars.usda.gov NR 49 TC 0 Z9 0 U1 7 U2 14 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND SN 0958-3157 EI 1360-0478 J9 BIOCONTROL SCI TECHN JI Biocontrol Sci. Technol. PD JUL PY 2016 VL 26 IS 7 BP 995 EP 1008 PG 14 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DN4GO UT WOS:000377022100009 ER PT J AU Libohova, Z Winzeler, HE Lee, B Schoeneberger, PJ Datta, J Owens, PR AF Libohova, Zamir Winzeler, Hans E. Lee, Brad Schoeneberger, Philip J. Datta, Jyotishka Owens, Phillip R. TI Geomorphons: Landform and property predictions in a glacial moraine in Indiana landscapes SO CATENA LA English DT Article DE Soil landscapes; Soil geomorphology; Geomorphons; Digital soil mapping; Soil attribute prediction; Multinomial logistics regression ID SOIL DRAINAGE CLASS; TERRAIN ATTRIBUTES; CLASSIFICATION; ECOSYSTEM; STOCKS; MODEL AB Predicting soil property distribution from a catena in the digital environment has been explored by many researchers with only slightly better than modest results. In this study, the landform recognition algorithm "geomorphons" in the GRASS GIS environment was explored to determine if this landscape model could improve predictions of soil properties. For 74 borings on the Wabash glacial moraine in Wells County, Indiana, measurements were made for: A horizon thickness, depth to chroma 2 features, effervescence, dense glacial till, carbonate concretions, and autochthonous platy structure. A digital elevation model (DEM) generated from light detection and ranging (LiDAR) data was used for the study site. The geomorphons algorithm was used to generate 10 original landforms: "flat", "footslope", "summit", "ridge", "shoulder", "spur", "slope", "hollow", "valley", and "depression" that were aggregated to new landforms coinciding with slope positions: "toeslope", "footslope", "backslope", "shoulder", "summit', and "depression" recognized by soil surveyors. Linear Discriminant Analysis (LDA) and Multinomial Logistics Regression Analysis (MLR) were used to aggregate the measured soil properties into the landform groups. The aggregation of geomorphons groups improved the MRL predictions to 83% accuracy. Also, the aggregation of geomorphons to five landforms to predict soil property distribution on the landscape gave promising results for the low-relief and relatively flat area of northeast Indiana. To test if the true mean value of each soil property for each landform was reliable for generalizing population characteristics, relative standard error (RSE) was calculated as a proportion of standard error to population mean from a bootstrap estimation. The range of RSE values for all soil properties and landforms was between similar to 0.7% and similar to 19%. Since the estimates of the measured soil properties all have RSE values of less than 25%, they can be considered reliable. (C) 2016 Elsevier B.V. All rights reserved. C1 [Libohova, Zamir; Schoeneberger, Philip J.] Nat Resources Conservat Serv, USDA, Natl Soil Survey Ctr, Lincoln, NE 68510 USA. [Winzeler, Hans E.; Owens, Phillip R.] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. [Lee, Brad] Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA. [Datta, Jyotishka] Purdue Univ, Dept Stat, W Lafayette, IN 47907 USA. RP Libohova, Z (reprint author), Nat Resources Conservat Serv, USDA, Natl Soil Survey Ctr, Lincoln, NE 68510 USA. EM zamir.libohova@lin.usda.gov NR 59 TC 0 Z9 0 U1 9 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD JUL PY 2016 VL 142 BP 66 EP 76 DI 10.1016/j.catena.2016.01.002 PG 11 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA DM9VQ UT WOS:000376714200006 ER PT J AU Robichaud, PR Wagenbrenner, JW Pierson, FB Spaeth, KE Ashmun, LE Moffet, CA AF Robichaud, Peter R. Wagenbrenner, Joseph W. Pierson, Fredrick B. Spaeth, Kenneth E. Ashmun, Louise E. Moffet, Corey A. TI Infiltration and interrill erosion rates after a wildfire in western Montana, USA SO CATENA LA English DT Article DE Ground cover; Post-fire recovery; Rainfall simulation; Sediment yield; Soil water repellency; Water drop penetration time ID SOIL-WATER REPELLENCY; COLORADO FRONT RANGE; LAND-USE TYPES; POSTFIRE RUNOFF; RAINFALL SIMULATION; HYDRAULIC CONDUCTIVITY; SEMIARID ENVIRONMENT; HILLSLOPE EROSION; WETTING PATTERNS; SIZE FRACTIONS AB The 2000 Valley Complex wildfire burned in steep montane forests with ash cap soils in western Montana, USA. The effects of high soil burn severity on forest soil hydrologic function were examined using rainfall simulations (100 mm h(-1) for 1 h) on 0.5-m(2) plots. Infiltration rates, sediment yields and sediment concentrations were compared among three treatments: control (unburned and undisturbed); bare (unburned with all surface vegetation, litter, and duff removed prior to each simulation); and burned. Rainfall simulations were done immediately after the fire and repeated in 2001, 2002, and 2005. Soil moisture, water repellency, and understory canopy and ground cover were measured and related to infiltration rates and sediment yields. The unburned forest soil was water repellent at the mineral surface. This surface repellency was no longer detected after it was burned at high severity, but a post-fire water repellent soil layer was observed at 1-2 cm below the surface. The control plots had high ground cover (90% overall), infiltration of 44-48 mm, and very low sediment concentrations (median values of 0.1-0.6 g L-1) and sediment yields (6-54 g m(-2)) for all years despite changes in soil moisture and strong water repellency. The bare and control plots had similar water repellency values, but the interrill erosion in the bare plots was high throughout the study (624-1277 g m(-2)) In the year of the fire, the burned sites had high rates of soil water repellency (88%) and little ground cover (10%). This resulted in low infiltration rates (30 mm), high sediment concentrations (median value 21 g L-1), and high sediment yields (1157 g m(-2)). By 2005, the fire-altered water repellency decreased in occurrence (48%) and severity, and the ground cover increased (42%). This resulted in much greater infiltration (84 mm), lower sediment concentration (median value 0.5 g L-1), and lower sediment yields (15 g m(-2)) on the burned plots. The importance of ground cover for preventing interrill erosion was demonstrated by the very low sediment yields on the control plots as compared to the bare and burned plots. The strength and occurrence of water repellency in both the unburned and burned sites decreased as soil moisture increased; however, strong soil water repellency was detected at the soil surface whenever unburned soils were dry. Fire-altered soil water repellency influenced the infiltration capacity and increased runoff rates immediately after the fire; however, the loss of protective ground cover was a more significant factor for the increased sediment concentrations and sediment yields. Published by Elsevier B.V. C1 [Robichaud, Peter R.; Wagenbrenner, Joseph W.; Ashmun, Louise E.] US Forest Serv, USDA, Rocky Mt Res Stn, 1221 South Main St, Moscow, ID 83843 USA. [Pierson, Fredrick B.; Moffet, Corey A.] ARS, USDA, Northwest Watershed Res Ctr, 800 East Pk Blvd,Plaza 4 Suite 105, Boise, ID 83712 USA. [Spaeth, Kenneth E.] Nat Resources Conservat Serv, USDA, Cent Natl Technol Support Ctr, Ft Worth, TX 76115 USA. [Wagenbrenner, Joseph W.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, 1400 Townsend Dr, Houghton, MI 49931 USA. [Moffet, Corey A.] ARS, USDA, Southern Plains Range Res Stn, 2000 18th St, Woodward, OK 73801 USA. RP Robichaud, PR (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, 1221 South Main St, Moscow, ID 83843 USA. EM probichaud@fs.fed.us; jwwagenb@mtu.edu; fred.pierson@ars.usda.gov; ken.spaeth@ftw.usda.gov; leashmun.work@gmail.com; corey.moffet@ars.usda.gov OI Moffet, Corey/0000-0002-7071-7539 FU U.S. Department of Agriculture (USDA), Forest Service and Department of Interior Joint Fire Science Program [01-3-02-08]; Rocky Mountain Research Station (USDA, Forest Service); Northwest Watershed Research Center (USDA, Agricultural Research Service) FX Funding for this project was provided in part by the U.S. Department of Agriculture (USDA), Forest Service and Department of Interior Joint Fire Science Program grant number 01-3-02-08 and the Rocky Mountain Research Station (USDA, Forest Service) and Northwest Watershed Research Center (USDA, Agricultural Research Service). The dedicated field crews from the Rocky Mountain Research Station and Northwest Watershed Research Center were essential to the success of this research. We thank the Bitterroot National Forest for their administrative and logistical support. NR 101 TC 4 Z9 4 U1 8 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD JUL PY 2016 VL 142 BP 77 EP 88 DI 10.1016/j.catena.2016.01.027 PG 12 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA DM9VQ UT WOS:000376714200007 ER PT J AU Estell, RE Anderson, DM James, DK AF Estell, R. E. Anderson, D. M. James, D. K. TI Defoliation of Flourensia cernua (tarbush) with high-density mixed-species stocking SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Biocontrol; Browsing; Cattle; Goats; Sheep; Shrubs ID SHEEP CONSUMING TOBOSA; LEAF SURFACE; LIVESTOCK HERBIVORY; EWE LAMBS; INGESTION; GRASSLAND; DESERT AB Interest in shrub use by livestock is increasing along with the rising demands placed on rangelands worldwide. Historically, Flourensia cernua (tarbush) has increased in the Chihuahuan Desert but receives limited use by cattle. Cattle, sheep and goats co-grazed eight 0.6 ha tarbush-dominated paddocks during two periods for up to nine days during two consecutive years. Cumulative tarbush defoliation across periods and years averaged 75.6%, with a mean increase of 9.3%/day (P < 0.0001). Defoliation of individual shrubs varied from 5 to 99% in 1989 and 0-100% in 1990, indicating highly variable palatability among individual plants. Sheep lost 2.3-5.5 kg/hd (P < 0.0001) across periods and years when forced to browse tarbush. In 1989, goats gained (P = 0.0345) 0.6 kg/hd in period 1, but the gain in period 2 was not significant (P = 0.2934). During 1990, goats lost 3.1 kg/hd (P = 0.0001) across periods. High-density mixed-species stocking of small areas for short time periods resulted in extensive tarbush use, primarily due to browsing by sheep and goats. Targeted use of tarbush for short time intervals may increase use of this highly nutritious forage and potentially serve as a form of biocontrol for this shrub. Published by Elsevier Ltd. C1 [Estell, R. E.; Anderson, D. M.; James, D. K.] USDA ARS, Jornada Expt Range, Las Cruces, NM 88003 USA. RP Estell, RE (reprint author), USDA ARS, Jornada Expt Range, Las Cruces, NM 88003 USA. EM restell@nmsu.edu NR 23 TC 0 Z9 0 U1 3 U2 3 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 EI 1095-922X J9 J ARID ENVIRON JI J. Arid. Environ. PD JUL PY 2016 VL 130 BP 62 EP 67 DI 10.1016/j.jaridenv.2016.03.013 PG 6 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DN1OB UT WOS:000376834200007 ER PT J AU Kluever, BM Gese, EM AF Kluever, Bryan M. Gese, Eric M. TI Spatial response of coyotes to removal of water availability at anthropogenic water sites SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Canid; Desert; Free water; Guzzler; Home-range ID WESTERN UNITED-STATES; GREAT-BASIN DESERT; HOME-RANGE SIZE; HABITAT SELECTION; KIT FOXES; CANIS-LATRANS; BIGHORN SHEEP; MANAGEMENT; WILDLIFE; CONSERVATION AB Features containing year-round availability of free water (hereafter water sites) and areas affiliated with water sites (i.e., riparian zones) occurring within arid landscapes represent a potential limiting resource for some desert dwelling vertebrates. Little is known about the relationship between water sites and mammalian carnivores. An increase of water sites in portions of the Great Basin Desert in Utah reportedly contributed to an increase in coyote (Canis latrans) abundance. We examined frequency of visitation and spatial affinity of resident coyotes for water sites at the home range scale extent. Visitation to sites with available water averaged 13.0 visitations/season (SD = 13.5) and ranged from zero to 47. We documented no visits to water sites in 16% (10 of 64) of seasonal home-ranges, <5 visits within 39% (25 of 64) of home ranges, and 25% (28 of 113) of coyote home-ranges did not contain a water site. Water sites associated with riparian vegetation experienced higher visitation than guzzlers (no riparian vegetation present). We found no evidence that removal of water influenced home range size or spatial shifting of home range areas. Water sites, especially guzzlers, do not represent a pivotal resource for the coyote population in our study area. (c) 2016 Elsevier Ltd. All rights reserved. C1 [Kluever, Bryan M.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Gese, Eric M.] Utah State Univ, Dept Wildland Resources, USDA,Natl Wildlife Res Ctr, Dept Agr,Anim & Plant Hlth Inspect Serv,Wildlife, Logan, UT 84322 USA. RP Kluever, BM (reprint author), Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. EM klueverb@gmail.com FU Department of Defense, U.S. Army Dugway Proving Ground, Environmental Programs, Dugway, Utah; U.S. Department of Agriculture, Wildlife Services, National Wildlife Research Center, Utah State University, Logan, Utah; Quinney College of Natural Resources, Utah State University, Logan, Utah; TE Inc; American Society of Mammalogists; Utah Chapter of the Wildlife Society; Utah Department of Natural Resources, Division of Wildlife Resources, Salt Lake City, Utah [100609] FX Funding and logistical support provided by the Department of Defense, U.S. Army Dugway Proving Ground, Environmental Programs, Dugway, Utah, and the U.S. Department of Agriculture, Wildlife Services, National Wildlife Research Center, Utah State University, Logan, Utah. Additional funding provided by the Quinney College of Natural Resources, Utah State University, Logan, Utah, T&E Inc, American Society of Mammalogists, the Utah Chapter of the Wildlife Society, and the Endangered Species Mitigation Fund of the Utah Department of Natural Resources, Division of Wildlife Resources, Salt Lake City (100609), Utah. We thank R. Knight for providing continual support for the project and review of the manuscript. We also thank B. Smith, A. Hodge, C. Crawford, L. Card, M. Cent, A. Reyer, C. Hansen, J. Fasig, W. Knowlton, W. Wright, C. Perkins, S. McEachin, J. DeCotis, and M. Richmond for field assistance. NR 77 TC 1 Z9 1 U1 6 U2 14 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 EI 1095-922X J9 J ARID ENVIRON JI J. Arid. Environ. PD JUL PY 2016 VL 130 BP 68 EP 75 DI 10.1016/j.jaridenv.2016.03.009 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DN1OB UT WOS:000376834200008 ER PT J AU Litman, JR Griswold, T Danforth, BN AF Litman, Jessica R. Griswold, Terry Danforth, Bryan N. TI Phylogenetic systematics and a revised generic classification of anthidiine bees (Hymenoptera: Megachilidae) SO MOLECULAR PHYLOGENETICS AND EVOLUTION LA English DT Article DE Megachilidae; Anthidiini; Phylogenetics; Integrative systematics; Nesting behavior ID WESTERN-HEMISPHERE HYMENOPTERA; SOUTHERN AFRICA; SEQUENCE DATA; MIXED MODELS; OLD-WORLD; APOIDEA; STELIS; BIOGEOGRAPHY; INFERENCE; ALIGNMENT AB The bee tribe Anthidiini (Hymenoptera: Megachilidae) is a large, cosmopolitan group of solitary bees that exhibit intriguing nesting behavior. We present the first molecular-based phylogenetic analysis of relationships within Anthidiini using model-based methods and a large, multi-locus dataset (five nuclear genes, 5081 base pairs), as well as a combined analysis using our molecular dataset in conjunction with a previously published morphological matrix. We discuss the evolution of nesting behavior in Anthidiini and the relationship between nesting material and female mandibular morphology. Following an examination of the morphological characters historically used to recognize anthidiine genera, we recommend the use of a molecular-based phylogenetic backbone to define taxonomic groups prior to the assignment of diagnostic morphological characters for these groups. Finally, our results reveal the paraphyly of numerous genera and have significant consequences for anthidiine classification. In order to promote a classification system based on stable, monophyletic clades, we hereby make the following changes to Michener's (2007) classification: The subgenera Afranthidium (Zosteranthidium) Michener and Griswold, 1994, Afranthidium (Branthidium) Pasteels, 1969 and Afranthidium (Immanthidium) Pasteels, 1969 are moved into the genus Pseudoanthidium, thus forming the new combinations Pseudoanthidium (Zosteranthidium), Pseudoanthidium (Branthidium), and Pseudoanthidium (Immanthidium). The genus Neanthidium Pasteels, 1969 is also moved into the genus Pseudoanthidium, thus forming the new combination Pseudoanthidium (Neanthidium). Based on morphological characters shared with our new definition of the genus Pseudoanthidium, the subgenus Afranthidium (Mesanthidiellum) Pasteels, 1969 and the genus Gnathanthidium Pasteels, 1969 are also moved into the genus Pseudoanthidium, thus forming the new combinations Pseudoanthidium (Mesanthidiellum) and Pseudoanthidium (Gnathanthidium). (C) 2016 Elsevier Inc. All rights reserved. C1 [Litman, Jessica R.] Nat Hist Museum Neuchatel, Terreaux 14, CH-2000 Neuchatel, Switzerland. [Griswold, Terry] Utah State Univ, USDA ARS, Bee Biol & Systemat Lab, Logan, UT 84322 USA. [Danforth, Bryan N.] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. RP Litman, JR (reprint author), Nat Hist Museum Neuchatel, Terreaux 14, CH-2000 Neuchatel, Switzerland. EM jessica.litman@unine.ch; terry.griswold@ars.usda.gov; bnd1@cornell.edu FU National Science Foundation [DEB-074299] FX This work was supported by a National Science Foundation grant to B.N. Danforth and T.L. Griswold (DEB-0742998, REVSYS: phylogeny and systematics of the megachilid bees). We thank M. Kuhlmann, C. Monnerat, A. Muller, L. Packer and C. Praz for providing specimens; M. Kuhlmann, L. Packer and C. Praz for helpful correspondence and conversation; and David Genoud and Entomologie/Botanik ETH Zurich for photographs. The authors declare no conflict of interest. NR 78 TC 2 Z9 2 U1 5 U2 12 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1055-7903 EI 1095-9513 J9 MOL PHYLOGENET EVOL JI Mol. Phylogenet. Evol. PD JUL PY 2016 VL 100 BP 183 EP 198 DI 10.1016/j.ympev.2016.03.018 PG 16 WC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity GA DN4RV UT WOS:000377055800016 PM 26988413 ER PT J AU Krishnankutty, SM Dietrich, CH Dai, W Siddappaji, MH AF Krishnankutty, Sindhu M. Dietrich, Christopher H. Dai, Wu Siddappaji, Madhura H. TI Phylogeny and historical biogeography of leafhopper subfamily Iassinae (Hemiptera: Cicadellidae) with a revised tribal classification based on morphological and molecular data SO SYSTEMATIC ENTOMOLOGY LA English DT Article ID SOUTHERN-HEMISPHERE; GENUS; HOMOPTERA; DISPERSAL; MADAGASCAR; PATTERNS; ORIGINS; FAUNA; CICADOMORPHA; VICARIANCE AB Phylogenetic relationships among major lineages of the leafhopper subfamily Iassinae were explored by analysing a dataset of 91 discrete morphological characters and DNA sequence data from nuclear 28SrDNA and histone H3 genes and mitochondrial 12SrDNA. Bayesian, maximum-likelihood and maximum parsimony analyses yielded similar tree topologies that were well resolved with strong branch support except at the base of the tree, resulting in equivocal support for inclusion of Bythoniini as a tribe of Iassinae but strong support for the monophyly of Iassinae (excluding Bythoniini) and most previously recognized iassine tribes. Divergence times for recovered nodes were estimated using a Bayesian relaxed clock method with two fossil calibration points. The results suggest that the deepest divergences coincided with Gondwanan vicariant events but that more recent divergences resulted from long-range dispersal and colonization. Biogeographical analyses suggest that the group most likely has a Neotropical origin. The following changes to the taxonomic classification are proposed: establishment of three new tribes, Batracomorphini trib.n. (based on type genus Batracomorphus Lewis), Hoplojassini trib.n. (based on type genus Hoplojassus Dietrich and including one other South American genus), Lipokrisnini trib.n. (based on type genus Lipokrisna Freytag and including two other endemic Caribbean genera); Krisnini is redefined to include only the Old World genera Krisna and Gessius; Iassini is redefined to include only the type genus and four endemic Afrotropical genera; Bascarrhinus Fowler and Platyhynna Berg, recently treated as genera incertae sedis, are placed in Hyalojassini; Thalattoscopus Kirkaldy is added to the previously monobasic tribe Trocnadini. Iassinae now includes 12 tribes, all of which appear to be monophyletic. Revised morphological diagnoses of the subfamily and each of the included tribes are provided and a key to tribes is also given. This published work has been registered in ZooBank, . C1 [Krishnankutty, Sindhu M.] Xavier Univ, USDA APHIS, Dept Biol, Cincinnati, OH 45207 USA. [Dietrich, Christopher H.; Siddappaji, Madhura H.] Illinois Nat Hist Survey, Champaign, IL 61820 USA. [Dai, Wu] Northwest A&F Univ, Yangling, Peoples R China. RP Dietrich, CH (reprint author), Univ Illinois, Illinois Nat Hist Survey, Prairie Res Inst, 1816 S Oak St, Champaign, IL 61820 USA. EM chdietri@illinois.edu FU National Science Foundation [DEB-9978026, 0529679]; NSF-China [31272343, 31572306]; Herbert Holdsworth Ross Memorial Award from Illinois Natural History Survey; School of Integrative Biology, University of Illinois FX We are grateful to the following individuals and institutions who provided many of the specimens used in this study: Jason Cryan (North Carolina Museum of Natural Sciences), Norman Penny (California Academy of Sciences), Michael Sharkey (University of Kentucky), Terry Erwin (National Museum of Natural History) and Michael Irwin (INHS). We also thank Pedro Lozada, Maria Cigliano and Ines Catalano for facilitating field work in Peru and Argentina. Funding was provided by National Science Foundation (grants DEB-9978026 and 0529679) to C.H. Dietrich; NSF-China grants 31272343 and 31572306 to W. Dai; Herbert Holdsworth Ross Memorial Award from Illinois Natural History Survey and the Clark Research Support Grant from the School of Integrative Biology, University of Illinois to S.K. None of the authors of this manuscript has declared any conflict of interest. NR 66 TC 1 Z9 1 U1 7 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0307-6970 EI 1365-3113 J9 SYST ENTOMOL JI Syst. Entomol. PD JUL PY 2016 VL 41 IS 3 BP 580 EP 595 DI 10.1111/syen.12175 PG 16 WC Evolutionary Biology; Entomology SC Evolutionary Biology; Entomology GA DN6ZG UT WOS:000377224500007 ER PT J AU Schisler, DA Slininger, PJ Olsen, NL AF Schisler, D. A. Slininger, P. J. Olsen, N. L. TI Appraisal of selected osmoprotectants and carriers for formulating Gram-negative biocontrol agents active against Fusarium dry rot on potatoes in storage SO BIOLOGICAL CONTROL LA English DT Article DE Biological control; Gram-negative; High throughput growth kinetics; Carriers; Efficacy; Dry rot ID GLYCINE BETAINE ACCUMULATION; PANTOEA-AGGLOMERANS CPA-2; BIOLOGICAL-CONTROL; PSEUDOMONAS-FLUORESCENS; FIRE BLIGHT; DESICCATION TOLERANCE; POSTHARVEST DISEASES; HEAD BLIGHT; OH 182.9; OSMOADAPTATION AB The production of a dry formulation containing a high titer of viable cells of a Gram-negative biological control agent is a challenging and critically important step in developing the agent into a commercial product. Producing a dry formulation using methods based on air-drying is especially attractive economically. Pseudomonas fluorescens strains S11P12, P22Y05, and S22T04 reduce Fusarium dry rot of potatoes in storage. The relative number of cells of each strain that survived air-drying after 24 h of cell growth in a liquid medium and treatment with carbohydrate-based osmoprotectants was determined using a high-throughput microtiter plate assay. Fructose and trehalose at 20 g L-1 were the most effective osmoprotectants when results were pooled across strains (P <= 0.05, FPLSD) though osmoprotectant influence on cell survival varied between strains. Different grades of diatomaceous earth, perlite, fumed silica and clay were then combined with suspensions of washed cells of each strain. After 15-18 h in an air-drier with relative humidity controlled at 50-60%, diatomaceous earth products Kenite (R) 700 and HYFLO (R) were superior in maintaining cell viability though, in the absence of osmoprotectants, log(10)CFU g (1) of these products dropped by 3 or more log units 24 h after drying. Air-dried products resulting from combining cells suspended in 20 g L (1) trehalose or fructose with Kenite (R) 700 and HYFLO (R) often reduced Fusarium dry rot by > 60% (P <= 0.05) for strains P22Y05 and S22T04 but S11P12 products were not as effective in laboratory assays. In a small pilot-scale test, dry formulations of these strains and the fungicide StadiumTM reduced dry rot decay by approximately 20% and 55%, respectively (P <= 0.05). Published by Elsevier Inc. C1 [Schisler, D. A.; Slininger, P. J.] USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. [Olsen, N. L.] Univ Idaho, Kimberly R&E Ctr, Kimberly, ID 83341 USA. RP Schisler, DA (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM David.Schisler@ars.usda.gov NR 47 TC 0 Z9 0 U1 4 U2 12 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD JUL PY 2016 VL 98 BP 1 EP 10 DI 10.1016/j.biocontrol.2016.03.009 PG 10 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DM5MZ UT WOS:000376394800001 ER PT J AU Kerr, YH Al-Yaari, A Rodriguez-Fernandez, N Parrens, M Molero, B Leroux, D Bircher, S Mahmoodi, A Mialon, A Richaume, P Delwart, S Al Bitar, A Pellarin, T Bindlish, R Jackson, TJ Rudiger, C Waldteufel, P Mecklenburg, S Wigneron, JP AF Kerr, Y. H. Al-Yaari, A. Rodriguez-Fernandez, N. Parrens, M. Molero, B. Leroux, D. Bircher, S. Mahmoodi, A. Mialon, A. Richaume, P. Delwart, S. Al Bitar, A. Pellarin, T. Bindlish, R. Jackson, T. J. Rudiger, C. Waldteufel, P. Mecklenburg, S. Wigneron, J. -P. TI Overview of SMOS performance in terms of global soil moisture monitoring after six years in operation SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE SMOS; Soil moisture; Level 2; Level 3; Neural networks; Retrieval accuracy metrics; Product inter-comparison; Validation ID VEGETATION OPTICAL DEPTH; RETRIEVAL ALGORITHM; WESTERN DENMARK; MICROWAVE EMISSION; DATA SETS; AMSR-E; VALIDATION; MODEL; NETWORK; SCALE AB The Soil Moisture and Ocean Salinity satellite (SMOS) was launched in November 2009 and started delivering data in January 2010. The commissioning phase ended in May 2010. Subsequently, the satellite has been in operation for over six years while the retrieval algorithms from Level 1 (L1) to Level 2 (L2) underwent significant evolutions as knowledge improved. Moreover, other approaches for retrieval at L2 over land were investigated while Level 3 (L3) and Level 4 (L4) were initiated. In this paper, these improvements were assessed by inter comparisons of the current L2 (V620) against the previous version (V551) and new products (using neural networks referred to as SMOS-NN) and 1.3 (referred to as SMOS-L3). In addition, a global evaluation of different SMOS soil moisture (SM) products (SMOS-L2, SMOS-L3, and SMOS-NN) was performed comparing products with those of model simulations and other satellites. Finally, all products were evaluated against in situ measurements of soil moisture (SM). To achieve such a goal a set of metrics to evaluate different satellite products are suggested. The study demonstrated that the V620 shows a significant improvement (including those at L1 improving L2) with respect to the earlier version V551. Results also show that neural network based approaches can often yield excellent results over areas where other products are poor. Finally, global compa'rison indicates that SMOS behaves very well when compared to other sensors/approaches and gives consistent results over all surfaces from very dry (African Sahel, Arizona), to wet (tropical rain forests). RFI (Radio Frequency Interference) is still an issue even though detection has been greatly improved through the significant reduction of RFI sources in several areas of the world. When compared to other satellite products, the analysis shows that SMOS achieves its expected goals and is globally consistent over different eco climate regions from low to high latitudes and throughout the seasons. (C) 2016 Elsevier Inc All rights reserved. C1 [Kerr, Y. H.; Rodriguez-Fernandez, N.; Parrens, M.; Molero, B.; Bircher, S.; Mahmoodi, A.; Mialon, A.; Richaume, P.; Al Bitar, A.] UT3, CNRS, CNES, CESBIO,UMR 5126,IRD, 18 Ave Edouard Belin, F-31401 Toulouse 9, France. [Al-Yaari, A.; Wigneron, J. -P.] INRA, ISPA, Bordeaux, France. [Leroux, D.; Pellarin, T.] Univ Grenoble Alpes, CNRS, LTHE, Grenoble, France. [Delwart, S.; Mecklenburg, S.] ESA, ESRIN, Frascati, Italy. [Bindlish, R.; Jackson, T. J.] USDA ARS, Belstville, MD USA. [Rudiger, C.] Monash Univ, Dept Civil Engn, Clayton, Vic 3168, Australia. [Waldteufel, P.] LATMOS, Paris, France. RP Kerr, YH (reprint author), 18 Ave Edouard Belin, F-31401 Toulouse 9, France. EM yann.kerr@cesbio.cnes.fr RI Al-Yaari, Amen/B-1444-2016; OI Al-Yaari, Amen/0000-0001-7530-6088; Leroux, Delphine/0000-0003-1688-021X; Parrens, Marie/0000-0001-7643-2211 FU CNES FX The authors are very grateful to all the teams dedicating time to collect ground data and to quality check them before making them available. Authors are also very grateful to all data users who provide feedback on data quality through their own Cal Val activities, enabling us to improve our products. The authors also wish to thank the reviewers for their very useful and in depth comments. Finally the authors wish to thank CNES who funds the CATDS activities as well as science through the TOSCA program. NR 98 TC 6 Z9 6 U1 17 U2 32 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD JUL PY 2016 VL 180 SI SI BP 40 EP 63 DI 10.1016/j.rse.2016.02.042 PG 24 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DN1BJ UT WOS:000376801000004 ER PT J AU Molero, B Merlin, O Malbeteau, Y Al Bitar, A Cabot, F Stefan, V Kerr, Y Bacon, S Cosh, MH Bindlish, R Jackson, TJ AF Molero, B. Merlin, O. Malbeteau, Y. Al Bitar, A. Cabot, F. Stefan, V. Kerr, Y. Bacon, S. Cosh, M. H. Bindlish, R. Jackson, T. J. TI SMOS disaggregated soil moisture product at 1 km resolution: Processor overview and first validation results SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Disaggregation; Soil moisture; Processor; SMOS; CATDS; Level 4 ID L-BAND RADIOMETER; AMSR-E; IN-SITU; SOUTHEASTERN AUSTRALIA; PERFORMANCE METRICS; MICROWAVE EMISSION; RADAR OBSERVATIONS; SMAP MISSION; SURFACE; STABILITY AB The SMOS (Soil Moisture and Ocean Salinity) mission provides surface soil moisture (SM) maps at a mean resolution of similar to 50 km. However, agricultural applications (irrigation, crop monitoring) and some hydrological applications (floods and modeling of small basins) require higher resolution SM information. In order to overcome this spatial mismatch, a disaggregation algorithm called Disaggregation based on Physical And Theoretical scale Change (DISPATCH) combines higher-resolution data from optical/thermal sensors with the SM retrieved from microwave sensors like SMOS, producing higher-resolution SM as the output. A DISPATCH-based processor has been implemented for the whole globe (emerged lands) in the Centre Aval de Traitement des Donnees SMOS (CATDS), the French data processing center for SMOS Level 3 products. This new CATDS Level-4 Disaggregation processor (C4DIS) generates SM maps at 1 km resolution. This paper provides an overview of the C4DIS architecture, algorithms and output products. Differences with the original DISPATCH prototype are explained and major processing parameters are presented. The C4DIS SM product is compared against L3 and in situ SM data during a one year period over the Murrumbidgee catchment and the Yanco area (Australia), and during a four and a half year period over the Little Washita and the Walnut Gulch watersheds (USA). The four validation areas represent highly contrasting climate regions with different landscape properties. According to this analysis, the C4DIS SM product improves the spatio-temporal correlation with in situ measurements in the semi-arid regions with substantial SM spatial variability mainly driven by precipitation and irrigation. In sub-humid regions like the Little Washita watershed, the performance of the algorithm is poor except for summer, as result of the weak moisture-evaporation coupling. Disaggregated products do not succeed to have and additional benefit in the Walnut Gulch watershed, which is also semi-arid but with well-drained soils that are likely to cancel the spatial contrast needed by DISPATCH. Although further validation studies are still needed to better assess the performance of DISPATCH in a range of surface and atmospheric conditions, the new C4DIS product is expected to provide satisfying results over regions having medium to high SM spatial variability. (C) 2016 Elsevier Inc. All rights reserved. C1 [Molero, B.; Merlin, O.; Malbeteau, Y.; Al Bitar, A.; Cabot, F.; Stefan, V.; Kerr, Y.; Bacon, S.] Ctr Etud Spatiales Biosphere, Toulouse, France. [Merlin, O.] Univ Cadi Ayyad, Marrakech, Morocco. [Cosh, M. H.; Bindlish, R.; Jackson, T. J.] USDA ARS, Hydrol & Remote Sensing Lab, Washington, DC 20250 USA. RP Molero, B (reprint author), Ctr Etud Spatiales Biosphere, Toulouse, France. EM beatriz.molero@cesbio.cnes.fr RI MALBETEAU, Yoann/E-5597-2017; Molero, Beatriz/O-5111-2014 OI MALBETEAU, Yoann/0000-0002-2964-6409; Molero, Beatriz/0000-0002-2781-8721 FU Australian Research Council [DP0343778, DP0557543]; CRC for Catchment Hydrology; CNES "Terre, Ocean, Surfaces Continentales, Atmosphere" program; French "Agence Nationale de la Recherche" MIXMOD-E project [ANR-13-JS06-0003-01] FX Initial setup and maintenance of the Murrumbidgee monitoring network used in this study was funded by the Australian Research Council (DP0343778, DP0557543) and by the CRC for Catchment Hydrology.; This study was supported by the CNES "Terre, Ocean, Surfaces Continentales, Atmosphere" program and by the French "Agence Nationale de la Recherche" MIXMOD-E project (ANR-13-JS06-0003-01). NR 75 TC 3 Z9 3 U1 15 U2 29 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD JUL PY 2016 VL 180 SI SI BP 361 EP 376 DI 10.1016/j.rse.2016.02.045 PG 16 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DN1BJ UT WOS:000376801000028 ER PT J AU McAllister, S Finney, M AF McAllister, Sara Finney, Mark TI The Effect of Wind on Burning Rate of Wood Cribs SO FIRE TECHNOLOGY LA English DT Article; Proceedings Paper CT Workshop of Fire-Structure Interaction and Large Outdoor Fires Operation Tomodachi: Fire Research CY MAR 16-18, 2015 CL Gaithersburg, MD DE Burning rate; Cribs; Forced ventilation; Wildland fire ID FIRES; VENTILATION AB Wood cribs are often used as ignition sources for room fire tests. A wood crib may also apply to studies of burning rate in wildland fires, because wildland fuel beds are porous and three dimensional. A unique aspect of wildland fires is the ubiquitous presence of wind. However, very little is known about what effect the increased ventilation has on the burning rate of cribs in either the densely- or loosely-packed regime. Experiments were performed with seven cribs designs with a range of porosities and two fuel element sizes: 0.64 cm and 1.27 cm. These cribs were burned in a wind tunnel with wind speeds ranging from 0 m/s to 0.7 m/s. Changes in the observed flame structure and burning patterns with wind are noted and discussed. The effect of wind on the burning rate was seen to depend on the fuel thickness. At the highest wind speed tested, cribs built with the 1.27 cm sticks showed a 6.5% to 61.5% increase in burning rate depending on porosity. Cribs built with the 0.64 cm sticks showed a decrease of 36.7% to 60.6% that was relatively constant with wind speed. Possible mechanisms of these changes are discussed. Future work will include further testing to clarify the causes of these trends. C1 [McAllister, Sara; Finney, Mark] US Forest Serv, USDA, RMRS Missoula Fire Sci Lab, 5775 W US Highway 10, Missoula, MT 59808 USA. RP McAllister, S (reprint author), US Forest Serv, USDA, RMRS Missoula Fire Sci Lab, 5775 W US Highway 10, Missoula, MT 59808 USA. EM smcallister@fs.fed.us FU National Fire Decision Support Center FX The authors wish to thank Sophia Vernholm for her tireless and careful construction of the cribs and Cyle Wold for setting up the data acquisition system. Funding for this work was provided by the National Fire Decision Support Center. NR 17 TC 1 Z9 1 U1 4 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0015-2684 EI 1572-8099 J9 FIRE TECHNOL JI Fire Technol. PD JUL PY 2016 VL 52 IS 4 SI SI BP 1035 EP 1050 DI 10.1007/s10694-015-0536-4 PG 16 WC Engineering, Multidisciplinary; Materials Science, Multidisciplinary SC Engineering; Materials Science GA DM1GJ UT WOS:000376093100006 ER PT J AU Ramsfield, TD Bentz, BJ Faccoli, M Jactel, H Brockerhoff, EG AF Ramsfield, T. D. Bentz, B. J. Faccoli, M. Jactel, H. Brockerhoff, E. G. TI Forest health in a changing world: effects of globalization and climate change on forest insect and pathogen impacts SO FORESTRY LA English DT Article DE biological invasions; climate change; forest resistance; modelling; risk; diagnostics ID IPS-TYPOGRAPHUS COLEOPTERA; EMERALD ASH BORER; RISK ANALYSIS; CEPHALCIA-ARVENSIS; UNITED-STATES; MANAGEMENT; EUROPE; PESTS; ALPS; CURCULIONIDAE AB Forests and trees throughout the world are increasingly affected by factors related to global change. Expanding international trade has facilitated invasions of numerous insects and pathogens into new regions. Many of these invasions have caused substantial forest damage, economic impacts and losses of ecosystem goods and services provided by trees. Climate change is already affecting the geographic distribution of host trees and their associated insects and pathogens, with anticipated increases in pest impacts by both native and invasive pests. Although climate change will benefit many forest insects, changes in thermal conditions may disrupt evolved life history traits and cause phenological mismatches. Individually, the threats posed to forest ecosystems by invasive pests and climate change are serious. Although interactions between these two drivers and their outcomes are poorly understood and hence difficult to predict, it is clear that the cumulative impacts on forest ecosystems will be exacerbated. Here we introduce and synthesize the information in this special issue of Forestry with articles that illustrate the impacts of invasions of insects and pathogens, climate change, forest management and their interactions, as well as methods to predict, assess and mitigate these impacts. Most of these contributions were presented at the XXIV IUFRO World Congress in 2014. C1 [Ramsfield, T. D.] Canadian Forest Serv, No Forestry Ctr, 5320-122 St NW, Edmonton, AB T6H 3S5, Canada. [Bentz, B. J.] USDA Forest Serv, Rocky Mt Res Stn, 860 N 1200 East, Logan, UT 84321 USA. [Faccoli, M.] Univ Padua, Dept Agron Food Nat Resources Animals & Environm, Viale Univ 6, I-35020 Legnaro, PD, Italy. [Jactel, H.] BIOGECO, INRA, UMR1202, Cestas, France. [Brockerhoff, E. G.] Scion New Zealand Forest Res Inst, POB 29237, Christchurch 8540, New Zealand. [Brockerhoff, E. G.] Better Border Biosecur Collaborat, Wellington, New Zealand. RP Ramsfield, TD (reprint author), Canadian Forest Serv, No Forestry Ctr, 5320-122 St NW, Edmonton, AB T6H 3S5, Canada. EM tod.ramsfield@canada.ca FU Canadian Forest Service; United States Department of Agriculture Forest Service; Ministry of Business Innovation and Employment via the Better Border Biosecurity Collaboration [C04X1104] FX The contribution of T.D.R. was funded by the Canadian Forest Service. Contributions by B.J.B. were supported by the United States Department of Agriculture Forest Service. Contributions by E.G.B. were supported by Ministry of Business Innovation and Employment core funding to Scion (contract C04X1104) via the Better Border Biosecurity Collaboration (www.b3nz.org). NR 71 TC 1 Z9 1 U1 38 U2 82 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0015-752X EI 1464-3626 J9 FORESTRY JI Forestry PD JUL PY 2016 VL 89 IS 3 SI SI BP 245 EP 252 DI 10.1093/forestry/cpw018 PG 8 WC Forestry SC Forestry GA DM1SV UT WOS:000376127500001 ER PT J AU Bentz, BJ Duncan, JP Powell, JA AF Bentz, Barbara J. Duncan, Jacob P. Powell, James A. TI Elevational shifts in thermal suitability for mountain pine beetle population growth in a changing climate SO FORESTRY LA English DT Article DE bark beetle; climate change; Dendroctonus ponderosae; demographic model; phenology; vulnerability ID WESTERN UNITED-STATES; DENDROCTONUS-PONDEROSAE COLEOPTERA; BARK BEETLES; SPATIOTEMPORAL PATTERNS; BRITISH-COLUMBIA; DEVELOPMENT TIME; ROCKY-MOUNTAINS; TREE MORTALITY; TEMPERATURE; SCOLYTIDAE AB Future forests are being shaped by changing climate and disturbances. Climate change is causing Large-scale forest declines globally, in addition to distributional shifts of many tree species. Because environmental cues dictate insect seasonality and population success, climate change is also influencing tree-killing bark beetles. The mountain pine beetle, Dendroctonus ponderosae, is a major disturbance in Pinus forests of the western US. Using a mechanistic, phenology-based demographic model driven by downscaled daily temperature data, we describe recent and future spatial and temporal thermal suitability for mountain pine beetle population growth in a topographically complex region. Trends in model-predicted growth rates among Global Climate Models were similar and suggest that, relative to future trends, mountain pine beetle population growth within the past 60 years was most optimal at middle elevations and Least optimal at the Lowest and highest elevations. This trend aligns with observed mountain pine beetle-caused tree mortality that was greatest at middle elevations between 1997 and 2013, as estimated from Aerial Detection Surveys. However, thermal suitability for optimal phenological synchrony was predicted to shift in recent years, and by the end of the century, the best thermal habitats for mountain pine beetle will be at the Lowest and highest elevations. Mechanistic demographic models are valuable tools for modelling future thermal regimes that may be both beneficial and maladaptive for mountain pine beetle population growth and subsequent tree mortality. C1 [Bentz, Barbara J.] USDA Forest Serv, Rocky Mt Res Stn, Logan, UT 84321 USA. [Duncan, Jacob P.; Powell, James A.] Utah State Univ, Dept Math & Stat, Logan, UT 84321 USA. RP Bentz, BJ (reprint author), USDA Forest Serv, Rocky Mt Res Stn, Logan, UT 84321 USA. EM bbentz@fs.fed.us FU USDA Forest Service; Western Environmental Threat Assessment Center; USDA Forest Service Rocky Mountain Research Station; USDA Forest Service, Region 1 Forest Health Protection FX This work was supported by the USDA Forest Service, Western Environmental Threat Assessment Center, USDA Forest Service Rocky Mountain Research Station, and USDA Forest Service, Region 1 Forest Health Protection. NR 69 TC 3 Z9 3 U1 28 U2 59 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0015-752X EI 1464-3626 J9 FORESTRY JI Forestry PD JUL PY 2016 VL 89 IS 3 SI SI BP 271 EP 283 DI 10.1093/forestry/cpv054 PG 13 WC Forestry SC Forestry GA DM1SV UT WOS:000376127500004 ER PT J AU Morin, RS Liebhold, AM AF Morin, Randall S. Liebhold, Andrew M. TI Invasive forest defoliator contributes to the impending downward trend of oak dominance in eastern North America SO FORESTRY LA English DT Article DE Lymantria dispar; gypsy moth; invasive pest; growth and mortality rates ID WHITE-TAILED DEER; GYPSY-MOTH; UNITED-STATES; LYMANTRIA-DISPAR; MORTALITY; IMPACT; JERSEY; FIRE AB Disturbance by non-native insect species can be an important ecological driver shaping Long-term changes in vegetation and plant species composition. While impacts of gypsy moth (Lymatria dispar L.) outbreaks in North American forests have been extensively studied, the results are quite inconsistent, particularly with respect to the amount of tree mortality associated with defoliation. In this study, we integrate geographical data describing historical gypsy moth defoliation with forest inventory data collected by a national forest inventory programme to quantify regional impacts across several million hectares of forest Land in the northeastern US. While observed increases in host tree mortality rates and decreases in growth rates associated with defoliation were expected, the study also indicates that this overstory mortality, coupled with ongoing declines in oak regeneration, will result in a Long-term reduction of oak density in defoliated areas. Eventually, these impacts will Likely contribute to regional shifts in tree species composition and forest succession pathways. Gypsy moth outbreaks thus appear to exacerbate ongoing declines in young oak age dosses in the region. C1 [Morin, Randall S.] USDA Forest Serv, No Res Stn, 11 Campus Blvd,Suite 200, Newtown Sq, PA 19073 USA. [Liebhold, Andrew M.] USDA Forest Serv, No Res Stn, 180 Canfield St, Morgantown, WV 26505 USA. RP Morin, RS (reprint author), USDA Forest Serv, No Res Stn, 11 Campus Blvd,Suite 200, Newtown Sq, PA 19073 USA. EM rsmorin@fs.fed.us NR 48 TC 3 Z9 3 U1 14 U2 37 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0015-752X EI 1464-3626 J9 FORESTRY JI Forestry PD JUL PY 2016 VL 89 IS 3 SI SI BP 284 EP 289 DI 10.1093/forestry/cpv053 PG 6 WC Forestry SC Forestry GA DM1SV UT WOS:000376127500005 ER PT J AU Weiland, JE Littke, WR Browning, JE Edmonds, RL Davis, A Beck, BR Miller, TW AF Weiland, Jerry E. Littke, Willis R. Browning, John E. Edmonds, Robert L. Davis, Anne Beck, Bryan R. Miller, Timothy W. TI Efficacy of reduced rate fumigant alternatives and methyl bromide against soilborne pathogens and weeds in western forest nurseries SO CROP PROTECTION LA English DT Article DE Soil fumigation; Methyl bromide alternatives; Pythium; Fusarium; Weed control; Forest nursery; Chloropicrin; Metam sodium; 1,3-dichloropropene ID DOUGLAS-FIR SEEDLINGS; BIOLOGICAL-CONTROL; FUSARIUM-OXYSPORUM; BIOCONTROL AGENTS; ROOT DISEASE; EPIDEMIOLOGY; SOIL AB Preplant soil fumigation is commonly used to control soilborne pathogens and weeds in forest seedling nurseries of Oregon and Washington. However, lower chemical inputs are desired to meet state and federal application regulations, to minimize buffer zone size requirements, and to help protect the environment. Therefore, the objectives of this research were to evaluate the efficacy of three reduced rate soil fumigants under totally impermeable film (TIF) in managing soilborne diseases and weeds, and to determine if combined applications of up to four biocontrol agents improved soilborne disease control. Reduced rates of methyl bromide, metam sodium, and 1,3-dichloropropene, all applied in combination with chloropicrin, were effective in decreasing soil populations of Pythium and Fusarium as well as the presence of Pythium in root debris from the previous crop. The roots of Douglas-fir (Pseudotsuga menziesii) seedlings transplanted into each fumigant treatment were also colonized less by Pythium and Fusarium than those transplanted into nonfumigated control plots. However, biocontrol treatments were not effective against either pathogen. Weed biomass and weeding times were also significantly reduced by fumigation. Application costs were similar for all three fumigant treatments, but seedling size was largest from the methyl bromide and metam sodium treatments followed by the 1,3-dichloropropene treatment. Based on the results of this study, reduced rates of methyl bromide, metam sodium, and 1,3-dichloropropene show promise in managing soilborne diseases and weeds in forest nurseries. Published by Elsevier Ltd. C1 [Weiland, Jerry E.; Davis, Anne; Beck, Bryan R.] USDA ARS, Corvallis, OR 97330 USA. [Littke, Willis R.; Browning, John E.] Weyerhaeuser Co, Federal Way, WA 98063 USA. [Edmonds, Robert L.] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98105 USA. [Miller, Timothy W.] Washington State Univ, Northwestern Washington Res & Extens Ctr, Mt Vernon, WA 98273 USA. RP Weiland, JE (reprint author), USDA ARS, Corvallis, OR 97330 USA. EM Jerry.Weiland@ars.usda.gov FU USDA-ARS Pacific Area Wide Pest Management Program for Methyl Bromide Alternatives; CRIS [2072-12220-004-00D] FX The authors thank Nathan Johnson with the University of Washington and Carol Larson and Glenn Cattnach with Weyerhaeuser for technical assistance. Financial support was provided by the USDA-ARS Pacific Area Wide Pest Management Program for Methyl Bromide Alternatives and CRIS Project No. 2072-12220-004-00D. NR 24 TC 0 Z9 0 U1 4 U2 15 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD JUL PY 2016 VL 85 BP 57 EP 64 DI 10.1016/j.cropro.2016.03.016 PG 8 WC Agronomy SC Agriculture GA DL7GP UT WOS:000375809300009 ER PT J AU Cheng, HN Ford, C Dowd, MK He, ZQ AF Cheng, H. N. Ford, Catrina Dowd, Michael K. He, Zhongqi TI Soy and cottonseed protein blends as wood adhesives SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Cottonseed protein; Soy protein; Protein blends; Polysaccharide filler; Adhesion; Wood veneer ID SODIUM DODECYL-SULFATE; GUANIDINE-HYDROCHLORIDE; CROSS-LINKING; STRENGTH; WATER; ARGININE; FORMALDEHYDE; COMPOSITES; FIBERBOARD; PRODUCTS AB As an environmentally friendlier alternative to adhesives from petroleum feedstock, soy proteins are currently being formulated as wood adhesives. Cottonseed proteins have also been found to provide good adhesive properties. In at least some cases, cottonseed proteins appear to form greater shear strength and improved hot water resistance compared with soy proteins. In the present study, blends of soy and cottonseed proteins were prepared, and their adhesive properties were found to decrease steadily with increased levels of soy protein in the formulations. In addition, cottonseed- and soy-protein based adhesives were also formulated with xylan, starch, or celluloses to determine the influence of polysaccharide fillers on protein-based adhesive properties. In some cases, adhesive shear strength was retained even when the cottonseed or soy protein was mixed with up to 75% polysaccharide. For cottonseed protein/polysaccharide formulations, hot water adhesive resistance. was retained when the blend contains about 50% polysaccharides. Soy protein formulations and its polysaccharide blends generally exhibited somewhat lower hot water resistance. In view of the ability of cottonseed protein/polysaccharide blends to retain shear strength and hot water resistance properties, these blends may provide an opportunity to decrease the amount of protein used in adhesive formulations, thereby decreasing cost. Published by Elsevier B.V. C1 [Cheng, H. N.; Ford, Catrina; Dowd, Michael K.; He, Zhongqi] ARS, So Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. RP Cheng, HN (reprint author), ARS, So Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM hn.cheng@ars.usda.gov NR 47 TC 3 Z9 3 U1 8 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD JUL PY 2016 VL 85 BP 324 EP 330 DI 10.1016/j.indcrop.2015.12.024 PG 7 WC Agricultural Engineering; Agronomy SC Agriculture GA DJ6ZG UT WOS:000374361100036 ER PT J AU Brito, B Konig, G Cabanne, GS Beascoechea, CP Rodriguez, L Perez, A AF Brito, Barbara Koenig, Guido Cabanne, Gustavo Sebastian Beascoechea, Claudia Perez Rodriguez, Luis Perez, Andres TI Phylogeographic analysis of the 2000-2002 foot-and-mouth disease epidemic in Argentina SO INFECTION GENETICS AND EVOLUTION LA English DT Article DE Foot-and-mouth disease; Viral transmission; Molecular epidemiology; Phylogeography; Argentina ID VIRUS; SEROTYPE; ACCURACY; DYNAMICS; OUTBREAK; MODEL; GENE AB Foot-and-mouth disease (FMD) is a highly transmissible disease of hooved livestock. Although FMD has been eradicated from many countries, economic and social consequences of FMD reintroductions are devastating. After achieving disease eradication, Argentina was affected by a major epidemic in 2000-2002, and within few months, FMD virus spread throughout most of the country and affected >2500 herds. Available records and viral strains allowed us to assess the origins, spread and progression of this FMD epidemic, which remained uncertain. We used whole genome viral sequences and a continuous phylogeographic diffusion approach, which revealed that the viruses that caused the outbreaks spread fast in different directions from a central area in Argentina. The analysis also suggests that the virus that caused the outbreaks in the year 2000 was different from those found during the 2001 epidemic. To estimate if the approximate overall genetic diversity of the virus was related to disease transmission, we reconstructed the viral demographic variation in time using Bayesian Skygrid approach and compared it with the epidemic curve and the within-herd transmission rate and showed that the genetic temporal diversity of the virus was associated with the increasing number of outbreaks in the exponential phase of the epidemic. Results here provide new evidence of how the disease entered and spread throughout the country. We further demonstrate that genetic data collected during a FMD epidemic can be informative indicators of the progression of an ongoing epidemic. (C) 2016 Elsevier B.V. All rights reserved. C1 [Brito, Barbara; Rodriguez, Luis] USDA ARS, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, POB 848, Greenport, NY 11944 USA. [Koenig, Guido; Cabanne, Gustavo Sebastian] INTA, Inst Biotecnol, Buenos Aires, DF, Argentina. [Cabanne, Gustavo Sebastian] Consejo Nacl Invest Cient & Tecn, Museo Argentino Ciencias Natur Bernardino Rivadav, RA-1033 Buenos Aires, DF, Argentina. [Beascoechea, Claudia Perez] DLA, Serv Nacl Sanidad & Calidad Agroalimentaria SENAS, OIE FMD Reference Lab, FMD Virol Dept, Buenos Aires, DF, Argentina. [Perez, Andres] Univ Minnesota, Coll Vet Med, Dept Vet Populat Med, St Paul, MN USA. RP Brito, B (reprint author), Univ Chile, Fac Ciencias Vet & Pecuarias, Dept Prevent Med, Av Santa Rosa, Santiago 11735, Chile. EM barbara.brito.r@gmail.com FU USDA-ARS Project "Developing predictive tools for prevention of Foot-and-Mouth Disease" [58-1940-7-124F]; CEEZAD; MnDrive FX This paper has been funded in part by grants from the USDA-ARS Project "Developing predictive tools for prevention of Foot-and-Mouth Disease" (58-1940-7-124F), CEEZAD, and the MnDrive. NR 31 TC 0 Z9 0 U1 4 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1567-1348 EI 1567-7257 J9 INFECT GENET EVOL JI Infect. Genet. Evol. PD JUL PY 2016 VL 41 BP 93 EP 99 DI 10.1016/j.meegid.2016.03.026 PG 7 WC Infectious Diseases SC Infectious Diseases GA DL5BU UT WOS:000375652800013 PM 27074336 ER PT J AU Nigatu, G AF Nigatu, Getachew TI Assessing the effects of climate change policy on the volatility of carbon prices in reference to the Great Recession SO JOURNAL OF ENVIRONMENTAL ECONOMICS AND POLICY LA English DT Article DE volatility; carbon markets; Great Recession ID GARCH MODELS; MARKETS; DYNAMICS; BUSINESS AB This paper examines the effects of having or not having a climate change policy on the behaviour of carbon price volatility before, within and after the 2008/09 global recession. Our Markov-regime switching model analysis shows that the voluntary carbon market at the Chicago Climate Exchange was in a high-volatile regime within, and two years before, this recession. The mandatory carbon market in the European Climate Exchange was relatively in stable and low-volatile regime over these periods, except at the end of the recession. The voluntary market exhibited more price volatility features than the mandatory one. After the recession, both markets experienced high probabilities of being at low-volatile regimes. Our results suggest that high-volatile regimes were not caused by the recession per se. However, statistical tests show that there were distinct low-and high-volatile regimes during the recession period, indicating that the recession aggravated price volatility of both markets. C1 [Nigatu, Getachew] USDA, Econ Res Serv, ERS, 355 E St SW, Washington, DC 20024 USA. RP Nigatu, G (reprint author), USDA, Econ Res Serv, ERS, 355 E St SW, Washington, DC 20024 USA. EM gsnigatu@ers.usda.gov NR 40 TC 0 Z9 0 U1 2 U2 3 PU ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXFORDSHIRE, ENGLAND SN 2160-6544 EI 2160-6552 J9 J ENVIRON ECON POLIC JI J. Environ. Econ. Policy PD JUL PY 2016 VL 5 IS 2 BP 200 EP 215 DI 10.1080/21606544.2015.1075906 PG 16 WC Environmental Studies SC Environmental Sciences & Ecology GA DK1GM UT WOS:000374660500004 ER PT J AU Ager, AA Day, MA Vogler, K AF Ager, Alan A. Day, Michelle A. Vogler, Kevin TI Production possibility frontiers and socioecological tradeoffs for restoration of fire adapted forests SO JOURNAL OF ENVIRONMENTAL MANAGEMENT LA English DT Article DE Forest restoration; Spatial optimization; Wildfire; Restoration prioritization; Ecosystems services; Restoration tradeoffs ID WILDLAND-URBAN INTERFACE; WESTERN NORTH-AMERICA; ECOSYSTEM SERVICES; PONDEROSA PINE; UNITED-STATES; ECOLOGICAL RESTORATION; LANDSCAPE RESTORATION; FUEL REDUCTION; SPOTTED OWL; MANAGEMENT AB We used spatial optimization to analyze alternative restoration scenarios and quantify tradeoffs for a large, multifaceted restoration program to restore resiliency to forest landscapes in the western US. We specifically examined tradeoffs between provisional ecosystem services, fire protection, and the amelioration of key ecological stressors. The results revealed that attainment of multiple restoration objectives was constrained due to the joint spatial patterns of ecological conditions and socioeconomic values. We also found that current restoration projects are substantially suboptimal, perhaps the result of compromises in the collaborative planning process used by federal planners, or operational constraints on forest management activities. The juxtaposition of ecological settings with human values generated sharp tradeoffs, especially with respect to community wildfire protection versus generating revenue to support restoration and fire protection activities. The analysis and methods can be leveraged by ongoing restoration programs in many ways including: 1) integrated prioritization of restoration activities at multiple scales on public and adjoining private lands, 2) identification and mapping of conflicts between ecological restoration and socioeconomic objectives, 3) measuring the efficiency of ongoing restoration projects compared to the optimal production possibility frontier, 4) consideration of fire transmission among public and private land parcels as a prioritization metric, and 5) finding socially optimal regions along the production frontier as part of collaborative restoration planning. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Ager, Alan A.] US Forest Serv, USDA, Rocky Mt Res Stn, Missoula Fire Sci Lab, 5775 US Highway 10W, Missoula, MT 59808 USA. [Day, Michelle A.] Oregon State Univ, Coll Forestry Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA. [Vogler, Kevin] Oregon State Univ, Coll Forestry Forest Engn Resources & Management, 043 Peavy Hall, Corvallis, OR 97331 USA. RP Ager, AA (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Missoula Fire Sci Lab, 5775 US Highway 10W, Missoula, MT 59808 USA. EM aager@fs.fed.us; michelle.day@oregonstate.edu; kevin.vogler@oregonstate.edu OI Vogler, Kevin/0000-0002-7080-2557 FU USDA Forest Service, Pacific Northwest Region, Portland, Oregon FX We are grateful to Stuart Brittain for providing programming support. We acknowledge Chris Ringo and Melanie Sutton for helping with the development of spatial data layers used in the study. This work was funded by the USDA Forest Service, Pacific Northwest Region, Portland, Oregon. NR 64 TC 1 Z9 1 U1 7 U2 24 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0301-4797 EI 1095-8630 J9 J ENVIRON MANAGE JI J. Environ. Manage. PD JUL 1 PY 2016 VL 176 BP 157 EP 168 DI 10.1016/j.jenvman.2016.01.033 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA DL2XU UT WOS:000375499300017 PM 27033166 ER PT J AU Feda, DM Roberts, AM Roemmich, JN AF Feda, Denise M. Roberts, April M. Roemmich, James N. TI Habituation to a stressor predicts adolescents' adiposity SO ANXIETY STRESS AND COPING LA English DT Article DE Adolescence; body mass index; habituation; obesity; stress ID DIETARY RESTRAINT; FOOD CHOICE; CHILDREN; OBESITY; SCHOOL; REACTIVITY; SNACKING; PAIN AB Background and Objectives: Stress is associated with gains in adiposity. One factor that determines how much stress is experienced is how quickly an adolescent reduces responding (habituates) across repeated stressors. The purpose of this study was to determine the association of body mass index (BMI) percentile and the rate of habituation to a stressor. Design: Thirty-four adolescents completed anthropometric measures and a habituation protocol using a within study design. Methods: The habituation protocol measured the rate of decline in perceived stress and heart rate (HR) across four, two-minute serial subtraction trials. Results: Multivariate linear regression revealed the habituation rate of the HR predicted BMI percentile after adjusting for gender, socioeconomic status, and initial HR (beta = 17.2, p<.04). Conclusions: Slower habituation to a laboratory stressor was associated with greater BMI percentiles in adolescents. C1 [Feda, Denise M.; Roberts, April M.] SUNY Buffalo, Dept Pediat, Buffalo, NY USA. [Roemmich, James N.] USDA, ARS, NPA, Grand Forks Human Nutr Ctr, Grand Forks, ND USA. RP Feda, DM (reprint author), SUNY Buffalo, Dept Pediat, Buffalo, NY USA. EM dmfeda@buffalo.edu FU University at Buffalo under the University at Buffalo Interdisciplinary Research Development Fund FX This work was supported by the University at Buffalo under the University at Buffalo 2020 Interdisciplinary Research Development Fund. The funding source had no involvement in the study design; collection, analysis and interpretation of data; in the writing of the report; or in the decision to submit the article for publication. NR 26 TC 0 Z9 0 U1 2 U2 5 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 1061-5806 EI 1477-2205 J9 ANXIETY STRESS COPIN JI Anxiety Stress Coping PD JUL PY 2016 VL 29 IS 4 BP 457 EP 462 DI 10.1080/10615806.2015.1065318 PG 6 WC Neurosciences; Psychiatry; Psychology, Multidisciplinary SC Neurosciences & Neurology; Psychiatry; Psychology GA DK5KW UT WOS:000374959800009 PM 26133077 ER PT J AU Benitez, MS Taheri, WI Lehman, RM AF Benitez, Maria-Soledad Taheri, Wendy I. Lehman, R. Michael TI Selection of fungi by candidate cover crops SO APPLIED SOIL ECOLOGY LA English DT Article DE Cover crops; Fungal communities; Arbuscular mycorrhizal fungi; Soil health; Pyrosequencing ID ARBUSCULAR MYCORRHIZAL FUNGI; MICROBIAL DIVERSITY; SOIL HEALTH; MICRODOCHIUM-NIVALE; COMMUNITY STRUCTURE; ECOSYSTEM SERVICES; ENDOPHYTIC FUNGI; PLANT-GROWTH; FUSARIUM; IDENTIFICATION AB Diversified cropping systems that incorporate year-round ground cover, are known to maintain healthy soils. Information is available for producers regarding the benefits of specific cover crop species for soil fertility, weed and pest management. Even though it is widely recognized that cover crops promote microbial biomass and activity, the specific responses of particular microbial groups to specific cover crop treatments are yet to be understood. In this work we determined differences in diversity and relative abundance of root endophytic fungi recovered by four potential cover crop species grown in a microberich soil inoculum. For this, a trap culture approach was used, were plants of four species were grown in the greenhouse until maturity in a soil mix originating from a prairie remnant. Root endophytic fungi were surveyed through amplicon sequencing of total root DNA, targeting universal fungal and arbuscular mycorrhizae (AM) - specific ribosomal markers. Each host species studied enriched for different fungal community assemblages, with particular fungal lineages being differentially abundant across hosts. In general, wheat enriched for the greatest diversity of fungi, whereas vetch and clover were best for enrichment of AM fungi and oat for saprotrophs. Specific groups of fungi more abundant in wheat roots included members of the Sordariomycetes, mainly represented by the families Lasiosphaeriaceae, Magnaporthaceae and the order Pleosporales; whereas vetch recovered greater abundance of Hypocreales-like sequences, highly represented by the genus Ilyonectria. Within AM fungi differences in taxa recovery were observed between the orders Glomerales and Diversisporales across hosts, being these groups more abundant in wheat and vetch roots, respectively. From a fungal perspective, the direct benefits of the cover crop species (or species mix) in the cash crop will relate to the life history characteristics of the fungal communities preferentially colonizing each cover crop host. Published by Elsevier B.V. C1 [Benitez, Maria-Soledad; Taheri, Wendy I.; Lehman, R. Michael] USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. RP Benitez, MS (reprint author), North Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. EM Soledad.BenitezPonce@ars.usda.gov RI Benitez, Maria/K-7777-2015 OI Benitez, Maria/0000-0003-2314-1052 FU South Dakota Corn Utilization Council FX We would like to thank the South Dakota Corn Utilization Council for supporting this research, Dr. James D. Bever for donating the AM fungal cultures used as PCR controls in this study and Dr. Rytas Vilgalys for sharing 454-tagged fungal primer aliquots. We thank Kurt Dagel and Shannon Osborne for helpful discussions on agronomic aspects of cover crops. NR 114 TC 0 Z9 0 U1 34 U2 74 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD JUL PY 2016 VL 103 BP 72 EP 82 DI 10.1016/j.apsoil.2016.03.016 PG 11 WC Soil Science SC Agriculture GA DK7RJ UT WOS:000375123100009 ER PT J AU Williams, A Davis, AS Ewing, PM Grandy, AS Kane, DA Koide, RT Mortensen, DA Smith, RG Snapp, SS Spokas, KA Yannarell, AC Jordan, NR AF Williams, Alwyn Davis, Adam S. Ewing, Patrick M. Grandy, A. Stuart Kane, Daniel A. Koide, Roger T. Mortensen, David A. Smith, Richard G. Snapp, Sieglinde S. Spokas, Kurt A. Yannarell, Anthony C. Jordan, Nicholas R. TI A comparison of soil hydrothermal properties in zonal and uniform tillage systems across the US Corn Belt SO GEODERMA LA English DT Article DE Nitrogen synchrony; Soil functional zone management; Soil moisture; Soil temperature ID FILLED PORE-SPACE; POORLY DRAINED SOIL; GROWING DEGREE-DAYS; PHYSICAL-PROPERTIES; RIDGE-TILLAGE; CONSERVATION TILLAGE; WATER-USE; CONVENTIONAL-TILLAGE; SOUTHWESTERN ONTARIO; PLANTING SYSTEMS AB Zonal tillage (e.g. ridge tillage, RT) separates management of row and inter-row positions, while non-zonal tillage (e.g. chisel plough, CP) applies management uniformly across a field. This may have large effects on soil hydro thermal properties, affecting soil processes and crop development. We examined the effects of RT versus CP on soil hydrothermal conditions under maize (Zea mays L) at four sites spanning the US Corn Belt over two growing seasons (2012-2013). We also investigated whether RT, as a result of changes in hydrothermal conditions, could stimulate greater soil nitrogen (N) availability during peak maize N demand. We captured wide variation in soil types and climates, allowing us to generalise tillage effects across a large environmental gradient. Continuous hydrothermal measurements were taken in the centre of row and inter-row positions. Soil cores collected shortly after maize six leaf stage (V6) were analysed for plant-available N and potentially mineralisable N (PMN). We hypothesised: 1) in spring CP and RT both produce warm, dry seedbeds with equivalent accumulations of growing degree days (GDD), but later in season RT holds greater soil moisture, providing better conditions for cover or relay crop establishment; 2) Hydrothermal properties of RT rows are distinct from RT inter-rows, while CP rows and inter-rows are indistinguishable; 3) RT promotes greater soil N mineralisation and availability in crop rows compared with CP. Results largely confirmed all hypotheses. In early spring, rows were drier in RT than CP, and both were similar in warmth (i.e. in accumulated GDD). From V6 to tasselling, CP accumulated more GDD than RT in inter-rows, while row positions remained similar; RT maintained greater soil moisture across both positions. From tasselling to harvest, RT inter-rows held greater soil moisture than CP, but accumulated fewer GDD. Both tillage systems showed zonation of soil moisture between planting and harvest (inter-rows moister than rows); the magnitude of zonation was greatest in RT. Plant-available N and PMN were greater in RT compared with CP at V6, suggesting RT increases synchrony of soil N availability with crop requirements. The results demonstrate that zonal tillage can integrate the seedbed benefits of conventional tillage with increased soil moisture retention across a wide range of climates and soil types. Increased moisture retention may help buffer agricultural systems against drought, and improve seedbed conditions for cover and relay crops in late summer and early autumn, thus potentially improving both sustainability and production in these systems. (C) 2016 The Authors. Published by Elsevier B.V. C1 [Williams, Alwyn; Ewing, Patrick M.; Jordan, Nicholas R.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Davis, Adam S.] USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. [Grandy, A. Stuart; Smith, Richard G.] Univ New Hampshire, Dept Nat Resources & Environm, Durham, NH 03824 USA. [Kane, Daniel A.; Snapp, Sieglinde S.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Koide, Roger T.] Brigham Young Univ, Dept Biol, Provo, UT 84602 USA. [Mortensen, David A.] Penn State Univ, Dept Plant Sci, University Pk, PA 16802 USA. [Spokas, Kurt A.] USDA ARS, Soil & Water Management Unit, St Paul, MN 55108 USA. [Yannarell, Anthony C.] Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA. RP Williams, A (reprint author), Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. EM alwyn.williams@outlook.com RI Spokas, Kurt/F-4839-2016 OI Spokas, Kurt/0000-0002-5049-5959 FU Agriculture and Food Research Initiative (AFRI) Climate Change Mitigation and Adaptation in Agriculture grant from the United States Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA) [2011-67003-30343] FX We would like to thank Vincent Filicetti, Sheri Huerd, Matt Peoples, Martin du Saire and Lee Yang for help in data collection. This project was supported by an Agriculture and Food Research Initiative (AFRI) Climate Change Mitigation and Adaptation in Agriculture grant (2011-67003-30343), from the United States Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA). The use of trade, firm, or corporation names in this paper is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the USDA or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. USDA is an equal opportunity provider and employer. NR 58 TC 0 Z9 0 U1 1 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD JUL 1 PY 2016 VL 273 BP 12 EP 19 DI 10.1016/j.geoderma.2016.03.010 PG 8 WC Soil Science SC Agriculture GA DL0ZJ UT WOS:000375361100002 ER PT J AU Solana, M Teel, J Hojilla-Evangelista, M Bertucco, A Eller, F AF Solana, M. Teel, J. Hojilla-Evangelista, M. Bertucco, A. Eller, F. TI Counter-current carbon dioxide extraction of fat from soy skim SO JOURNAL OF SUPERCRITICAL FLUIDS LA English DT Article DE Carbon dioxide; Counter-current extraction; Soy skim; Fat; Proteins ID SUPERCRITICAL CO2; SOYBEAN OIL; SELECTIVE EXTRACTION; AQUEOUS EXTRACTION; ETHANOL; FRACTIONATION; MIXTURES; HEXANE; SEED AB This research aims to investigate the use of counter-current carbon dioxide extraction method as a means to reduce residual fat in soy skim after the enzyme-assisted aqueous extraction of soybeans. Extractions with liquid CO2 at 25 degrees C and 10.34 MPa and supercritical CO2 at 50 degrees C and 25.16 MPa are compared. The effects of solvent-to-feed ratio (2.5-10), addition of ethanol as a modifier (5% w/w) and introductions of packing in the column are also analyzed. Results show that the highest reduction of fat content is obtained without modifier and with packing in the column at 50 degrees C and 25.16 MPa. At these conditions, the total fat content present in soy skim was reduced from 4.4 to 0.7%, with the protein content practically unaffected. ANOVA was applied to determine effects on fat and protein in soy skim, being the addition of packing in the column the most significant one. The fatty acid profile was also analyzed, with C18:2 being the predominant fatty acid present in all the soy skim samples. Gel electrophoresis indicated that supercritical CO2 settings did not affect the polypeptide band patterns; however, higher pressure, temperature, and CO2 flow significantly increased protein solubility in aqueous media (pH 2-10) compared with un-treated samples. (C) 2016 Elsevier B.V. All rights reserved. C1 [Solana, M.; Bertucco, A.] Univ Padua, Dept Ind Engn, Via Marzolo 9, I-35131 Padua, Italy. [Solana, M.; Teel, J.; Eller, F.] USDA ARS, Natl Ctr Agr Utilizat Res, Funct Foods Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. [Hojilla-Evangelista, M.] USDA ARS, Natl Ctr Agr Utilizat Res, Plant Polymer Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. RP Solana, M (reprint author), Univ Padua, Dept Ind Engn, Via Marzolo 9, I-35131 Padua, Italy.; Solana, M (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, Funct Foods Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM miriam.solanacipres@studenti.unipd.it FU Cassa di Risparmio del Veneto Foundation FX M. Solana gratefully acknowledges the doctoral scholarship for foreign students from Cassa di Risparmio del Veneto Foundation. NR 25 TC 0 Z9 0 U1 7 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0896-8446 EI 1872-8162 J9 J SUPERCRIT FLUID JI J. Supercrit. Fluids PD JUL PY 2016 VL 113 BP 106 EP 111 DI 10.1016/j.supflu.2016.03.022 PG 6 WC Chemistry, Physical; Engineering, Chemical SC Chemistry; Engineering GA DL2YB UT WOS:000375500000015 ER PT J AU Domke, GM Perry, CH Walters, BF Woodall, CW Russell, MB Smith, JE AF Domke, Grant M. Perry, Charles H. Walters, Brian F. Woodall, Christopher W. Russell, Matthew B. Smith, James E. TI Estimating litter carbon stocks on forest land in the United States SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE United Framework Convention on Climate Change; Greenhouse gas; National forest inventory; Forest floor; Carbon budget ID LINKING CLIMATE; SITE INDEX; DECOMPOSITION; SOIL; DYNAMICS; QUALITY; MODEL; RATES; PINE; ECOSYSTEMS AB Forest ecosystems are the largest terrestrial carbon sink on earth, with more than half of their net primary production moving to the soil via the decomposition of litter biomass. Therefore, changes in the litter carbon (C) pool have important implications for global carbon budgets and carbon emissions reduction targets and negotiations. Litter accounts for an estimated 5% of all forest ecosystem carbon stocks worldwide. Given the cost and time required to measure litter attributes, many of the signatory nations to the United Nations Framework Convention on Climate Change report estimates of litter carbon stocks and stock changes using default values from the Intergovernmental Panel on Climate Change or country-specific models. In the United States, the country-specific model used to predict litter C stocks is sensitive to attributes on each plot in the national forest inventory, but these predictions are not associated with the litter samples collected over the last decade in the national forest inventory. Here we present, for the first time, estimates of litter carbon obtained using more than 5000 field measurements from the national forest inventory of the United States. The field-based estimates mark a 44% reduction (2081 +/- 77 Tg) in litter carbon stocks nationally when compared to country-specific model predictions reported in previous United Framework Convention on Climate Change submissions. Our work suggests that Intergovernmental Panel on Climate Change defaults and country-specific models used to estimate litter carbon in temperate forest ecosystems may grossly overestimate the contribution of this pool in national carbon budgets. Published by Elsevier B.V. C1 [Domke, Grant M.; Perry, Charles H.; Walters, Brian F.; Woodall, Christopher W.] US Forest Serv, USDA, No Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. [Russell, Matthew B.] Univ Minnesota, Dept Forest Resources, 1530 Cleveland Ave N, St Paul, MN 55108 USA. [Smith, James E.] US Forest Serv, USDA, No Res Stn, 271 Mast Rd, Durham, NH 03824 USA. RP Domke, GM (reprint author), US Forest Serv, USDA, No Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. EM gmdomke@fs.fed.us OI Domke, Grant/0000-0003-0485-0355; Perry, Charles/0000-0001-9377-5996 NR 54 TC 1 Z9 2 U1 3 U2 31 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD JUL 1 PY 2016 VL 557 BP 469 EP 478 DI 10.1016/j.scitotenv.2016.03.090 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK7VZ UT WOS:000375136200049 PM 27017077 ER PT J AU Moreira, WH Tormena, CA Karlen, DL da Silva, AP Keller, T Betioli, E AF Moreira, Wagner Henrique Tormena, Cassio Antonio Karlen, Douglas L. da Silva, Alvaro Pires Keller, Thomas Betioli, Edner, Jr. TI Seasonal changes in soil physical properties under long-term no-tillage SO SOIL & TILLAGE RESEARCH LA English DT Article DE Soil physical quality; Soil porosity; Soil structure ID LIMITING WATER RANGE; CROP PRODUCTION; BULK-DENSITY; HYDRAULIC CONDUCTIVITY; LOAMY SAND; COMPACTION; QUALITY; RESILIENCE; RETENTION; SYSTEMS AB There is no consensus regarding seasonal changes in soil physical properties within and between rows in long-term no-till (NT) crop production systems. We hypothesized that soil physical properties in a Rhodic Ferralsol under long-term NT differed within and between rows and that these changes are influenced by wetting and drying cycles (WDC). Undisturbed samples were taken within and between crop rows from layers of 0 to 0.10 and 0.10 to 0.20 m depth in September 2010, 2011 and 2012 and March 2012 and 2013. At the first sampling, 40 soil samples were collected within the maize (Zea mays L.) row (R), at interrow (IR) sampling positions, and at an intermediate position (IP) between R and IR. Coordinates for each sampling point were identified so that subsequent samples could be collected from the same location. Soil bulk density (Db), soil water retention curve (WRC), S index, air-entry pressure and pore size distribution were determined. The results confirmed that furrow opening causes significant positive changes in soil physical properties within the crop row and plant growth can be affected by the "confinement" of roots within the R position within long-term NT sites. With each successive sampling, Db decreased and was significantly influenced by recent WDC. The pore size distribution showed larger pores with each successive sampling, providing a higher S index, air-entry pressure, and improved soil physical quality over time. The steady state of soil structural conditions achieved at long term NT can be affected by short term influences related to the crops and weather conditions. However, soil physical properties indicated that a new equilibrium was achieved and that soil under long-term NT may remain physically functional. Our results confirm that soil physical properties under NT are highly dynamic and strongly influenced by (i) soil disturbance caused by furrow opening, (ii) wetting and drying cycles, and (iii) sampling depth. Therefore, we recommend that for quantifying soil physical quality within no-till fields, measurements should be taken within and between crop rows. (C) 2016 Elsevier B.V. All rights reserved. C1 [Moreira, Wagner Henrique; Betioli, Edner, Jr.] Univ Estadual Maringa, Postgrad Program Agron, Av Colombo 5790, BR-87020900 Maringa, Parana, Brazil. [Tormena, Cassio Antonio] Univ Estadual Maringa, Dept Agron, Av Colombo 5790, BR-87020900 Maringa, Parana, Brazil. [Karlen, Douglas L.] ARS, USDA, Natl Lab Agr & Environm, 2110 Univ Blvd, Ames, IA 50011 USA. [da Silva, Alvaro Pires] Univ Sao Paulo, ESALQ, Dept Soil & Plant Nutr, Av Padua Dias 11,Caixa Postal 9, BR-13418900 Sao Paulo, Brazil. [Keller, Thomas] Agroscope, Dept Nat Resources & Agr, Reckenholzstr 191, CH-8046 Zurich, Switzerland. [Keller, Thomas] Swedish Univ Agr Sci, Dept Soil & Environm, Box 7014, SE-75007 Uppsala, Sweden. RP Moreira, WH (reprint author), Univ Estadual Maringa, Postgrad Program Agron, Av Colombo 5790, BR-87020900 Maringa, Parana, Brazil. EM wh.moreira@hotmail.com RI da Silva, Alvaro Pires/C-5973-2012 OI da Silva, Alvaro Pires/0000-0002-1544-7310 FU CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico), Brazilian Agency for Research FX The authors are grateful to CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico), Brazilian Agency for Research, for providing scholarship and research grants. NR 55 TC 2 Z9 2 U1 17 U2 43 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-1987 EI 1879-3444 J9 SOIL TILL RES JI Soil Tillage Res. PD JUL PY 2016 VL 160 BP 53 EP 64 DI 10.1016/j.still.2016.02.007 PG 12 WC Soil Science SC Agriculture GA DK3FJ UT WOS:000374802200006 ER PT J AU Brandon, DL Korn, AM AF Brandon, David L. Korn, Anna M. TI Immunosorbent analysis of toxin contamination in milk and ground beef using IgY-based ELISA SO FOOD AND AGRICULTURAL IMMUNOLOGY LA English DT Article DE ELISA; IgY; ricin; Shiga toxin ID SHIGA-LIKE TOXIN; RIBOSOME-INACTIVATING PROTEINS; EGG-YOLK ANTIBODY; IMMUNO-PCR ASSAY; ESCHERICHIA-COLI; CHICKEN EGG; MONOCLONAL-ANTIBODIES; RICIN CONTAMINATION; FOOD; AGGLUTININS AB Analytical methodology to detect ricin and Shiga toxins (Stx) in food matrices is important for food safety and biosecurity. Monoclonal antibodies (mAbs) that bind each toxin were used for capture in sandwich enzyme-linked immunosorbent assay, and IgY polyclonal antibodies were prepared as detection antibodies. The ricin assay systems, using colorimetric or chemiluminescent substrates, detected native, but not heat-denatured ricin. The lower limit of detection (LOD) was 0.13ngmL(-1) in milk and 0.8ngg(-1) in ground beef. The Stx2 assay systems detected native Stx2, but not heat-denatured Stx2 or Stx1. The LOD was 0.13ngmL(-1) in milk and 0.7ngg(-1) in ground beef. Using a standard 96-well-plate format, the assays can detect less than 1x10(-4) of an estimated lethal oral dose of either toxin in a serving of milk. The IgY detection antibodies for ricin were more heat-stable than mouse polyclonal anti-ricin at 65 degrees C. C1 [Brandon, David L.; Korn, Anna M.] ARS, USDA, Foodborne Toxin Detect & Prevent Res Unit, Western Reg Res Ctr, Albany, CA USA. RP Brandon, DL (reprint author), ARS, USDA, Foodborne Toxin Detect & Prevent Res Unit, Western Reg Res Ctr, Albany, CA USA. EM davidbrandon@post.harvard.edu FU Agricultural Research Service National Research Program 108 [2030-42000-048] FX This work was conducted under Project 2030-42000-048 of Agricultural Research Service National Research Program 108 (Food Safety). NR 48 TC 0 Z9 0 U1 9 U2 16 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0954-0105 EI 1465-3443 J9 FOOD AGR IMMUNOL JI Food Agric. Immunol. PD JUL PY 2016 VL 27 IS 4 BP 496 EP 508 DI 10.1080/09540105.2015.1126809 PG 13 WC Chemistry, Applied; Food Science & Technology; Immunology; Toxicology SC Chemistry; Food Science & Technology; Immunology; Toxicology GA DK1SH UT WOS:000374693800006 ER PT J AU Huang, M Kim, MS Delwiche, SR Chao, K Qin, JW Mo, C Esquerre, C Zhu, QB AF Huang, Min Kim, Moon S. Delwiche, Stephen R. Chao, Kuanglin Qin, Jianwei Mo, Changyeun Esquerre, Carlos Zhu, Qibing TI Quantitative analysis of melamine in milk powders using near-infrared hyperspectral imaging, and band ratio SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Food safety; Hyperspectral imaging; Milk powder; Melamine; Band ratio; Image classification ID CYANURIC ACID; LIQUID-CHROMATOGRAPHY; DAIRY-PRODUCTS; INFANT FORMULA; ANIMAL FEED; SPECTROSCOPY; ADULTERATION; SYSTEM; CATS AB Since 2008, the detection of the adulterant melamine (2,4,6-triamino-1,3,5-triazine) in food products has become the subject of research due to several food safety scares. Near-infrared (NIR) hyperspectral imaging offers great potential for food safety and quality research because it combines the features of vibrational spectroscopy and digital imaging. In this study, NIR hyperspectral imaging was investigated for quantitative evaluation of melamine particles in nonfat and whole milk powders. Melamine was mixed into milk powders in a concentration range of 0.02-1.00% (w/w). A NIR hyperspectral imaging system was used to acquire images (938-1654 nm) of melamine powder, whole milk powder, nonfat milk powder, and mixtures of melamine and each of the milk powders. Two optimal bands (1447 nm and 1466 nm) were selected by a linear correlation algorithm with pure milk and pure melamine. Band ratio (13144711455) images coupled with a single threshold were used to create resultant images to visualize identification and distribution of the melamine adulterant particles in milk powders. The identification results were verified by spectral feature comparison between separated mean spectra of melamine pixels and milk pixels. Linear correlations (r) were found between the number of pixels identified as containing melamine and melamine concentration in nonfat milk and whole milk powders, which were 0.980 and 0.970 or higher, respectively. The study demonstrated that the combination of NIR hyperspectral imaging and simple band ratioing was promising for rapid quantitative analysis of melamine in milk powders. Published by Elsevier Ltd. C1 [Huang, Min; Zhu, Qibing] Jiangnan Univ, Key Lab Adv Proc Control Light Ind, Minist Educ, Wuxi 214122, Peoples R China. [Kim, Moon S.; Chao, Kuanglin; Qin, Jianwei] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. [Delwiche, Stephen R.] ARS, Food Qual Lab, USDA, Bldg 303,BARC East, Beltsville, MD 20705 USA. [Mo, Changyeun] Rural Dev Adm, Natl Acad Agr Sci, Jueonju Si 560500, Jeollabuk Do, South Korea. [Esquerre, Carlos] Univ Dublin, Dept Biosyst Engn, Dublin, Ireland. RP Delwiche, SR (reprint author), ARS, Food Qual Lab, USDA, Bldg 303,BARC East, Beltsville, MD 20705 USA. EM stephen.delwiche@ars.usda.gov RI Esquerre Fernandez, Carlos Alberto/F-2182-2011 OI Esquerre Fernandez, Carlos Alberto/0000-0002-0917-1522 FU USDA-ARS; Agenda Program [PJ009399]; Rural Development Administration, Suwon, Korea; National Natural Science Foundation of China [61271384, 61275155]; 111 Project of China [B12018]; Qing Lan Project FX In addition to base funding from the USDA-ARS, this work was supported by a grant from the Agenda Program (PJ009399), Rural Development Administration, Suwon, Korea, the National Natural Science Foundation of China (Grant no. 61271384, 61275155), the 111 Project (B12018) of China, and the Qing Lan Project. NR 30 TC 2 Z9 2 U1 20 U2 58 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 EI 1873-5770 J9 J FOOD ENG JI J. Food Eng. PD JUL PY 2016 VL 181 BP 10 EP 19 DI 10.1016/j.jfoodeng.2016.02.017 PG 10 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA DJ6XV UT WOS:000374357400002 ER PT J AU Lu, YZ Li, RC Lu, RF AF Lu, Yuzhen Li, Richard Lu, Renfu TI Structured-illumination reflectance imaging (SIRI) for enhanced detection of fresh bruises in apples SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Structured illumination; Reflectance imaging; Apple; Fruit; Defect; Bruise ID FREQUENCY-DOMAIN; TISSUE; MEDIA AB A structured-illumination reflectance imaging (SIRI) system was developed for the detection of fresh bruises in apples. The system consisted of a high-performance digital camera, a broadband DC-regulated light source, and a digital light engine for generating specific patterns of illumination. Sinusoidal patterns of illumination at a series of spatial frequencies were created to illuminate test samples. Experiments were first conducted on a strongly scattering nylon sample embedded with foreign objects at different depths and then on apples of three cultivars with fresh bruises. The acquired images were demodulated into two separate sets of direct component (representing uniform diffuse illumination) and alternating component (or amplitude) images for each spatial frequency. The amplitude images were found to be effective for ascertaining the sub-surface defects, and the detection resolution and depth depended on the spatial frequency of illumination. SIRI technique was able to detect fresh bruises in apples immediately after they were induced, which, otherwise, could be difficult to achieve by conventional uniform illumination-based imaging techniques. The technique is promising for effective detection of defects, like bruise, in fruit. Published by Elsevier B.V. C1 [Lu, Yuzhen; Li, Richard] Michigan State Univ, Dept Biosyst & Agr Engn, E Lansing, MI 48824 USA. [Lu, Renfu] Michigan State Univ, USDA, ARS, E Lansing, MI 48824 USA. RP Lu, RF (reprint author), Michigan State Univ, 524 S Shaw Lane,Room 224, E Lansing, MI 48824 USA. EM luyuzhen@msu.edu; lirichar@msu.edu; renfu.lu@ars.usda.gov NR 14 TC 5 Z9 5 U1 3 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD JUL PY 2016 VL 117 BP 89 EP 93 DI 10.1016/j.postharvbio.2016.02.005 PG 5 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA DI7YJ UT WOS:000373717600011 ER PT J AU Janisiewicz, WJ Nichols, B Bauchan, G Chao, TC Jurick, WM AF Janisiewicz, Wojciech J. Nichols, Breyn Bauchan, Gary Chao, Thomas C. Jurick, Wayne M., II TI Wound responses of wild apples suggest multiple resistance mechanism against blue mold decay SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Wild apple germplasm; Resistance to Penicillium; Postharvest decay ID PENICILLIUM-EXPANSUM; BOTRYTIS-CINEREA; HEAT-TREATMENT; POSTHARVEST DISEASES; BIOLOGICAL-CONTROL; FRUIT FIRMNESS; INFECTION; DIGITATUM; PATHOGEN; DEFENSE AB Blue mold caused primarily by Penicillium expansum and to a lesser extent other Penicillium spp. is the most destructive disease of stored apples in the US and worldwide. It was recently shown that resistance to blue mold exists in wild apple germplasms, Malus sieversii, from Kazakhstan and in other species from different regions maintained as a collection in Geneva, NY. We initiated studies to determine the durability and the mechanism(s) of resistance to P. expansum in select wild apple accessions. Wound responses (up to 96 h in 24 h intervals), affecting P. expansum infection, and related cytological changes were determined in accessions with varying levels of resistance. In general, the more resistant the accession, the quicker the wound response that prevented the fungus from infecting tissue and causing decay. No decay developed on immune apple accessions, even when inoculated immediately after wounding at the inoculum concentration of 10(5) conidia/mL. On a moderately resistant accession, a 24 h interval between wounding and inoculation was sufficient to avert decay. Reactive oxygen species (ROS) were detected at high levels immediately after wounding in the immune as well as susceptible accessions. Callose and lignin/suberin appears to play a minor role in resistance responses. Our results indicate the presence of a high level of durable resistance/immunity in the wild apples which is governed by several mechanism(s). This presents a new challenge for explaining the observed resistance and at the same time creates an opportunity for exploiting these resistant mechanisms in breeding programs to incorporate resistance to fruit decays into commercial cultivars. Published by Elsevier B.V. C1 [Janisiewicz, Wojciech J.; Nichols, Breyn] ARS, Appalachian Fruit Res Stn, USDA, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. [Jurick, Wayne M., II] ARS, Food Qual Lab, Henry A Wallace Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Bauchan, Gary] ARS, Electron & Confocal Microscopy Unit, Henry A Wallace Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Chao, Thomas C.] ARS, Plant Genet Resources Unit, USDA, Geneva, NY 14456 USA. RP Janisiewicz, WJ (reprint author), ARS, Appalachian Fruit Res Stn, USDA, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM Wojciech.Janisiewicz@ars.usda.gov FU USDA [CRIS 8080-22000-009-00D] FX We would like to acknowledge the help of Mr. Bill Srmack in maintaining the collection of wild apple plants and assistance in collecting fruit samples. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA; USDA is an equal opportunity provider and employer. This research was sponsored by USDA CRIS 8080-22000-009-00D. NR 51 TC 0 Z9 0 U1 15 U2 30 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD JUL PY 2016 VL 117 BP 132 EP 140 DI 10.1016/j.postharvbio.2015.12.004 PG 9 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA DI7YJ UT WOS:000373717600017 ER PT J AU Liu, F Majeed, H Antoniou, J Li, Y Ma, Y Yokoyama, W Ma, JG Zhong, F AF Liu, Fei Majeed, Hamid Antoniou, John Li, Yue Ma, Yun Yokoyama, Wallace Ma, Jianguo Zhong, Fang TI Tailoring physical properties of transglutaminase-modified gelatin films by varying drying temperature SO FOOD HYDROCOLLOIDS LA English DT Article DE Gelatin films; Transglutaminase; Cross-linking; Drying temperature; Physical properties ID CROSS-LINKED GELATIN; FISH SKIN GELATIN; MECHANICAL-PROPERTIES; THERMOMECHANICAL PROPERTIES; THERMAL-PROPERTIES; COMPOSITE FILMS; BARRIER; HYDROGELS; LINKING; GELS AB Transglutaminase (TGase)-modified gelatin films containing different levels of physical and chemical networks were prepared by drying films above or below gelation temperature of gelatin. Differences in protein network structure were observed by optical microscopy analysis in freeze-dried film-forming solutions. The relative amount of triple helices decreased when drying temperature increased as observed by X-ray diffraction (XRD) and differential scanning calorimetry (DSC). The addition of TGase slightly inhibited triple helix formation. TGase-modified films exhibited stronger mechanical properties than blank films, and the highest tensile strength was observed in films dried close to the gelation temperature (25 degrees C) and the highest elongation at break above it (35 degrees C). TGase modification enhanced the water resistance and thermal stability of gelatin films by decreasing the water solubility, increasing the glass transition temperature and degradation temperature, respectively, which was further enhanced with increasing drying temperature. The cross-section (SEM) and surface (AFM) microstructure of gelatin films with TGase both appeared more compact and smooth, and improved when temperature increased. The results obtained show that drying temperature may be used to tailor the physical properties of TGase-modified gelatin films for specific applications. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Liu, Fei; Majeed, Hamid; Antoniou, John; Li, Yue; Ma, Yun; Ma, Jianguo; Zhong, Fang] Jiangnan Univ, Sch Food Sci & Technol, Minist Educ, Key Lab Food Colloids & Biotechnol, Wuxi 214122, Peoples R China. [Liu, Fei; Yokoyama, Wallace] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Zhong, F (reprint author), Jiangnan Univ, Sch Food Sci & Technol, Minist Educ, Key Lab Food Colloids & Biotechnol, Wuxi 214122, Peoples R China. EM fzhong@jiangnan.edu.cn FU National 125 Program [2011BAD23B02, 2013AA102207]; National Natural Science Foundation of China [31171686, 31401532, 31571891]; Natural Science Foundation of Jiangsu Province [BK2012556]; 111 Project [B07029, PCSIRT0627, JUSRP11422, JUSRP51507] FX This work was financially supported by National 125 Program 2011BAD23B02, 2013AA102207, National Natural Science Foundation of China 31171686, 31401532 and 31571891, the Natural Science Foundation of Jiangsu Province BK2012556, 111 Project B07029, PCSIRT0627, JUSRP11422 and JUSRP51507. NR 37 TC 3 Z9 3 U1 11 U2 47 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0268-005X EI 1873-7137 J9 FOOD HYDROCOLLOID JI Food Hydrocolloids PD JUL PY 2016 VL 58 BP 20 EP 28 DI 10.1016/j.foodhyd.2016.01.026 PG 9 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DI0EP UT WOS:000373168200003 ER PT J AU Lehtonen, M Teraslahti, S Xu, C Yadav, MP Lampi, AM Mikkonen, KS AF Lehtonen, Mari Teraslahti, Suvi Xu, Chunlin Yadav, Madhav P. Lampi, Anna-Maija Mikkonen, Kirsi S. TI Spruce galactoglucomannans inhibit lipid oxidation in rapeseed oil-in-water emulsions SO FOOD HYDROCOLLOIDS LA English DT Article DE Spruce galactoglucomannans; Carboxymethylation; Emulsion stability; Lipid oxidation ID OLEIC SUNFLOWER TRIACYLGLYCEROLS; ANTIOXIDANT ACTIVITY; NORWAY SPRUCE; THERMAL POLYMERIZATION; PHENOLIC-COMPOUNDS; GAMMA-TOCOPHEROLS; STABILITY; FILMS; GUM; POLYSACCHARIDES AB Oil-in-water (O/W) emulsions are functional and highly significant systems in industrial settings. Their large interfacial area makes them prone to deterioration, including the oxidation of polyunsaturated lipids. Spruce galactoglucomannans (GGM), which are wood biomacromolecules that are abundantly available from emerging forestry biorefineries, were characterized as novel, bio-based, sustainable stabilizers against lipid oxidation in rapeseed O/W emulsions. The formation of hydroperoxides, volatile oxidation products, and polymerized lipids were followed in emulsions during an accelerated storage test at 40 degrees C for two weeks. The free and bound phenolic residues associated with plant polysaccharides were quantified to explain the differences in the oxidation pathways of emulsions stabilized with GGM and their carboxymethyl derivatives (CMGGM) in comparison to the "golden standard" food stabilizer, gum arabic (GA), and a promising alternative, corn fiber gum (CFG). GGM and CMGGM efficiently inhibited and altered the pathways of lipid oxidation of rapeseed oil. GGM showed an exceptional capacity to inhibit lipid oxidation and act as a multifunctional stabilizer, enhancing both the physical and oxidative sility of emulsions. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Lehtonen, Mari; Teraslahti, Suvi; Lampi, Anna-Maija; Mikkonen, Kirsi S.] Univ Helsinki, Dept Food & Environm Sci, POB 27, FIN-00014 Helsinki, Finland. [Xu, Chunlin] Abo Akad Univ, Lab Wood & Paper Chem, Johan Gadolin Proc Chem Ctr, FI-20500 Turku, Finland. [Yadav, Madhav P.] ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. RP Mikkonen, KS (reprint author), Univ Helsinki, Dept Food & Environm Sci, POB 27, FIN-00014 Helsinki, Finland. EM mari.lehtonen@helsinki.fi; suvi.teraslahti@gmail.com; cxu@abo.fi; madhav.yadav@ars.usda.gov; anna-maija.lampi@helsinki.fi; kirsi.s.mikkonen@helsinki.fi RI Xu, Chunlin/H-5224-2012; OI Xu, Chunlin/0000-0003-1860-9669; Mikkonen, Kirsi/0000-0003-4490-5912 FU Academy of Finland [268144] FX The authors gratefully acknowledge the Academy of Finland (Project No. 268144) for its funding. The authors also wish to thank Tuuli Koivumaki for her assistance with the UPLC-DAD-FLD. NR 59 TC 2 Z9 2 U1 7 U2 24 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0268-005X EI 1873-7137 J9 FOOD HYDROCOLLOID JI Food Hydrocolloids PD JUL PY 2016 VL 58 BP 255 EP 266 DI 10.1016/j.foodhyd.2016.03.006 PG 12 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DI0EP UT WOS:000373168200027 ER PT J AU Harde, SM Wang, Z Horne, M Zhu, JY Pan, X AF Harde, S. M. Wang, Z. Horne, M. Zhu, J. Y. Pan, X. TI Microbial lipid production from SPORL-pretreated Douglas fir by Mortierella isabellina SO FUEL LA English DT Article DE Forest residue; Microbial lipid; SPORL pretreatment; Mortierella isabellina; Detoxification ID SINGLE-CELL OIL; ENHANCE ENZYMATIC SACCHARIFICATION; CELLULOSIC ETHANOL-PRODUCTION; YEAST CRYPTOCOCCUS-CURVATUS; OLEAGINOUS YEAST; CORN STOVER; TRICHOSPORON-FERMENTANS; YARROWIA-LIPOLYTICA; LIPOMYCES-STARKEYI; FOREST RESIDUE AB The solid substrate and spent liquor obtained after SPORL pretreatment of Douglas fir residues were evaluated as substrates for the production of intracellular microbial lipid by Mortierella isabellina NRRL 1757. The production of lipid was investigated in a batch fermentation using different strategies viz. separate hydrolysis and fermentation, quasi-simultaneous saccharification and fermentation (Q-SSF), and whole slurry (solid substrate and spent liquor) saccharification and fermentation. The maximum lipid yield (0.21 g/g sugar) was obtained with Q-SSF of the solid substrate. Nutrient supplementation was not necessary for lipid production from SPORL-pretreated Douglas fir. Detoxification of SPORL spent liquor with lime did remove the inhibitors and improved the fermentation for lipid production. The lipid produced had a similar fatty acid compositional profile to those of vegetable oil, suggesting that it could have potential as a substrate for biodiesel production. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Harde, S. M.; Wang, Z.; Horne, M.; Zhu, J. Y.; Pan, X.] Univ Wisconsin, Dept Biol Syst Engn, 460 Henry Mall, Madison, WI 53706 USA. [Zhu, J. Y.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Pan, X (reprint author), Univ Wisconsin, Dept Biol Syst Engn, 460 Henry Mall, Madison, WI 53706 USA. EM xpan@wisc.edu FU WARF (Wisconsin Alumni Research Foundation); Graduate School at the University of Wisconsin-Madison FX This work was supported by WARF (Wisconsin Alumni Research Foundation) and the Graduate School at the University of Wisconsin-Madison. NR 62 TC 1 Z9 1 U1 4 U2 28 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0016-2361 EI 1873-7153 J9 FUEL JI Fuel PD JUL 1 PY 2016 VL 175 BP 64 EP 74 DI 10.1016/j.fuel.2016.02.023 PG 11 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA DG1NB UT WOS:000371833700008 ER PT J AU Katam, R Sakata, K Suravajhala, P Pechan, T Kambiranda, DM Naik, KS Guo, BZ Basha, SM AF Katam, Ramesh Sakata, Katsumi Suravajhala, Prashanth Pechan, Tibor Kambiranda, Devaiah M. Naik, Karamthot Sivasankar Guo, Baozhu Basha, Sheikh M. TI Comparative leaf proteomics of drought-tolerant and -susceptible peanut in response to water stress SO JOURNAL OF PROTEOMICS LA English DT Article DE Drought tolerance; Leaf proteins; Protein-protein interaction; Two-dimensional electrophoresis; Water stress ID PREHARVEST AFLATOXIN CONTAMINATION; ARACHIS-HYPOGAEA L.; GENE-EXPRESSION; OSMOTIC-STRESS; PROTEINS; RICE; LEAVES; PLANTS; WHEAT; IDENTIFICATION AB Water stress (WS) predisposes peanut plants to fungal infection resulting in pre-harvest aflatoxin contamination. Major changes during water stress including oxidative stress, lead to destruction of photosynthetic apparatus and other macromolecules within cells. Two peanut cultivars with diverse drought tolerance characteristics were subjected to WS, and their leaf proteome was compared using two-dimensional electrophoresis complemented with MALDI-TOF/FOF mass spectrometry. Ninety-six protein spots were differentially abundant to water stress in both cultivars that corresponded to 60 non-redundant proteins. Protein interaction prediction analysis suggests that 42 unique proteins showed interactions in tolerant cultivar while 20 showed interactions in the susceptible cultivar, activating other proteins in directed system response networks. Four proteins: glutamine ammonia ligase, chitin class II, actin isoform B, and beta tubulin, involved in metabolism, defense and cellular biogenesis, are unique in tolerant cultivar and showed positive interactions with other proteins. In addition, four proteins: serine/threonine protein phosphate PP1, choline monooxygenase, peroxidase 43, and SNFl-related protein kinase regulatory subunit beta-2, that play a role as cryoprotectants through signal transduction, were induced in drought tolerant cultivar following WS. Eleven interologs of these proteins were found in Arabidopsis interacting with several proteins and it is believed that similar mechanisms/pathways exist in peanut. Significance: Peanuts (Arachis hypogaea L) are a major source of plant protein grown in subtropical and tropical regions of the world. Pre-harvest aflatoxin contamination is a major problem that affects peanut crop yield and food safety. Poor understanding of molecular and cellular mechanisms associated with aflatoxin resistance is largely responsible for the lack of progress in elucidating a process/methodology for reducing aflatoxin contamination in peanuts. Drought perturbs the invasion of the aflatoxin producing fungus and thus affects the quality and yield of peanut. Therefore, more studies involving the effects of drought stress to determine the molecular changes will enhance our understanding of the key metabolic pathways involved in the combined stresses. The changes associated with the biotic and abiotic interactions within the peanut will be used to determine the metabolic pathways involved in the stress tolerance. This research would be beneficial in identifying the tolerant molecular signatures and promoting food safety and consumer health through breeding superior quality peanut cultivars. (C) 2016 Elsevier B.V. All rights reserved. C1 [Katam, Ramesh] Florida A&M Univ, Dept Biol Sci, Tallahassee, FL 32307 USA. [Sakata, Katsumi] Maebashi Inst Technol, Dept Life Sci & Informat, 460-1 Kamisadori, Maebashi, Gumma 3710816, Japan. [Suravajhala, Prashanth] Bioclues Org, Hyderabad, Andhra Pradesh, India. [Pechan, Tibor] Mississippi State Univ, Mississippi Agr & Forestry Expt Stn, Inst Genom Biocomp & Biotechnol, Mississippi State, MS 39759 USA. [Kambiranda, Devaiah M.; Basha, Sheikh M.] Florida A&M Univ, Coll Agr & Food Sci, Tallahassee, FL 32307 USA. [Naik, Karamthot Sivasankar] ANGR Agr Univ, Guntur, AP, India. [Guo, Baozhu] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. RP Katam, R (reprint author), Florida A&M Univ, Dept Biol Sci, Coll Sci & Technol, 1530 MLK Blvd, Tallahassee, FL 32307 USA. EM ramesh.katam@gmail.com OI Suravajhala, Prashanth/0000-0002-8535-278X FU National Science Foundation-Research Experience for Undergraduate Students [1156900]; Faculty Research Award Program [002257]; Japan Society for the Promotion of Science [510187, S-14088] FX RK thankfully acknowledges the financial support from National Science Foundation-Research Experience for Undergraduate Students (1156900) and Faculty Research Award Program (002257) to conduct this research, and to Japan Society for the Promotion of Science (510187, S-14088) to support collaborative efforts. Dr. Barry L Tillman, UF/IFAS, Mariana FL provided peanut germplasm. Ms. Kaylee Castle proof read the manuscript and Ms. Khadijah Weathers assisted in graphics. Authors are thankful to Professor Frank B. Matta, Mississippi State University for providing financial support for Protein sequencing. NR 84 TC 2 Z9 2 U1 14 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1874-3919 EI 1876-7737 J9 J PROTEOMICS JI J. Proteomics PD JUN 30 PY 2016 VL 143 SI SI BP 209 EP 226 DI 10.1016/j.jprot.2016.05.031 PG 18 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA DT1HD UT WOS:000381231400020 PM 27282920 ER PT J AU Wei, L Link, TE Hudak, AT Marshall, JD Kavanagh, KL Abatzoglou, JT Zhou, H Pangle, RE Flerchinger, GN AF Wei, Liang Link, Timothy E. Hudak, Andrew T. Marshall, John D. Kavanagh, Kathleen L. Abatzoglou, John T. Zhou, Hang Pangle, Robert E. Flerchinger, Gerald N. TI Simulated water budget of a small forested watershed in the continental/maritime hydroclimatic region of the United States SO HYDROLOGICAL PROCESSES LA English DT Article DE streamflow; transpiration; LiDAR; sap flux; soil water storage; model test ID MIXED-CONIFER FORESTS; TEMPERATE RAIN-FOREST; RESIDUE-SOIL SYSTEM; SIMULTANEOUS HEAT; PACIFIC-NORTHWEST; ROCKY-MOUNTAINS; MODEL; SURFACE; PRECIPITATION; ELEVATION AB Annual streamflows have decreased across mountain watersheds in the Pacific Northwest of the United States over the last similar to 70years; however, in some watersheds, observed annual flows have increased. Physically based models are useful tools to reveal the combined effects of climate and vegetation on long-term water balances by explicitly simulating the internal watershed hydrological fluxes that affect discharge. We used the physically based Simultaneous Heat and Water (SHAW) model to simulate the inter-annual hydrological dynamics of a 4km(2) watershed in northern Idaho. The model simulates seasonal and annual water balance components including evaporation, transpiration, storage changes, deep drainage, and trends in streamflow. Independent measurements were used to parameterize the model, including forest transpiration, stomatal feedback to vapour pressure, forest properties (height, leaf area index, and biomass), soil properties, soil moisture, snow depth, and snow water equivalent. No calibrations were applied to fit the simulated streamflow to observations. The model reasonably simulated the annual runoff variations during the evaluation period from water year 2004 to 2009, which verified the ability of SHAW to simulate the water budget in this small watershed. The simulations indicated that inter-annual variations in streamflow were driven by variations in precipitation and soil water storage. One key parameterization issue was leaf area index, which strongly influenced interception across the catchment. This approach appears promising to help elucidate the mechanisms responsible for hydrological trends and variations resulting from climate and vegetation changes on small watersheds in the region. Copyright (c) 2015 John Wiley & Sons, Ltd. C1 [Wei, Liang; Link, Timothy E.; Marshall, John D.; Kavanagh, Kathleen L.] Univ Idaho, Dept Forest Rangeland & Fire Sci, Moscow, ID 83844 USA. [Hudak, Andrew T.] US Forest Serv, USDA, Rocky Mt Res Stn, 1221 S Main St, Moscow, ID 83843 USA. [Marshall, John D.] Swedish Agr Univ, Dept Forest Ecol & Management, S-90736 Umea, Sweden. [Kavanagh, Kathleen L.] Dept Ecosyst Sci & Management, Hort & Forest Sci 305, MS 2138, College Stn, TX 77843 USA. [Abatzoglou, John T.; Zhou, Hang] Univ Idaho, Dept Geog, Moscow, ID 83844 USA. [Pangle, Robert E.] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. [Flerchinger, Gerald N.] USDA ARS, Northwest Watershed Res Ctr, 800 Pk Blvd,Suite 105, Boise, ID 83712 USA. [Wei, Liang] Univ Missouri, Dept Forestry, Columbia, MO 65211 USA. RP Wei, L (reprint author), Univ Idaho, Dept Forest Rangeland & Fire Sci, Moscow, ID 83844 USA.; Wei, L (reprint author), Univ Missouri, Dept Forestry, Columbia, MO 65211 USA. EM liangwei@uidaho.edu OI Abatzoglou, John/0000-0001-7599-9750; Wei, Liang/0000-0001-8967-5036 FU U.S. Forest Service Western Wildlands Environmental Threat Assessment Center (WWETAC); NASA New Investigator Program [NNX14AC26G] FX Funding was provided by the U.S. Forest Service Western Wildlands Environmental Threat Assessment Center (WWETAC) and to the University of Idaho through a Joint Venture Agreement (13-JV-11221633-130). Additional support was provided by the NASA New Investigator Program (NNX14AC26G). We thank Robert Denner for providing streamflow data, and Ina Sue Miller, Tom Black, and Charlie Luce for climate data. NR 54 TC 0 Z9 0 U1 12 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD JUN 30 PY 2016 VL 30 IS 13 BP 2000 EP 2013 DI 10.1002/hyp.10769 PG 14 WC Water Resources SC Water Resources GA DR2MW UT WOS:000379739600002 ER PT J AU Rinkevich, FD Margotta, JW Pittman, JM Ottea, JA Healy, KB AF Rinkevich, Frank D. Margotta, Joseph W. Pittman, Jean M. Ottea, James A. Healy, Kristen B. TI Pteridine levels and head weights are correlated with age and colony task in the honey bee, Apis mellifera SO PEERJ LA English DT Article DE Honey bee; Pteridines; Polyethism; Fluorescence ID DIVISION-OF-LABOR; HYPOPHARYNGEAL GLAND; HYMENOPTERA; INSECTS AB Background. The age of an insect strongly influences many aspects of behavior and reproduction. The interaction of age and behavior is epitomized in the temporal polyethism of honey bees in which young adult bees perform nurse and maintenance duties within the colony, while older bees forage for nectar and pollen. Task transition is dynamic and driven by colony needs. However, an abundance of precocious foragers or overage nurses may have detrimental effects on the colony. Additionally, honey bee age affects insecticide sensitivity. Therefore, determining the age of a set of individual honey bees would be an important measurement of colony health. Pteridines are purine-based pigment molecules found in many insect body parts. Pteridine levels correlate well with age, an wild caught insects maybe accurate age by measuring pteridine levels. The relationship between pteridines and age varies with a number of internal and external factors among many species. Thus far, no studies have investigated the relationship of.. pteridines with age in honey bees. Methods. We established single-cohort colonies to obtain age-matched nurse and forager bees. Bees of known ages were also sampled from colonies with normal demographics. Nurses and foragers were collected every 3-5 days for up to 42 days. Heads were removed and weighed before pteridines were purified and analyzed using previously established fluorometric methods. Results. Our analysis showed that pteridine levels significantly increased with age in a linear manner in both single cohort colonies and colonies with normal demography. Pteridine levels were higher in foragers than nurses of the same age in bees from single cohort colonies. Head weight significantly increased with age until approximately 28-days of age and then declined for both nurse and forager bees in single cohort colonies. A similar pattern of head weight in bees from colonies with normal demography was observed but head weight was highest in 8-day old nurse bees and there was no relationship of head weight with age of foragers. Discussion. Although the relationship between pteridine levels and age was significant, variation in the data yielded a +4-day range in age estimation, This allows an unambiguous method to determine whether a bee may be a young nurse or old forager in colonies with altered demographics as in the case of single cohort colonies. Pteridine levels in bees do not correlate with age as well as in other insects. However, most studies used insects reared under tightly controlled laboratory conditions, while we used free-living bees, The dynamics of head weight change with age is likely to be due to growth and atrophy of the hypopharyngeal glands. Taken together, these methods represent a useful tool for assessing the age of an insect. Future studies utilizing these methods will provide a more holistic view of colony health. C1 [Rinkevich, Frank D.; Margotta, Joseph W.; Pittman, Jean M.; Ottea, James A.; Healy, Kristen B.] Louisiana State Univ, Ctr Agr, Dept Entomol, Baton Rouge, LA 70803 USA. [Rinkevich, Frank D.] USDA ARS, Honey Bee Breeding Genet & Physiol Res Lab, Baton Rouge, LA USA. RP Healy, KB (reprint author), Louisiana State Univ, Ctr Agr, Dept Entomol, Baton Rouge, LA 70803 USA. EM KHealy@agcenter.lsu.edu FU USDA [6050-21000-014-03S] FX This research was funded by a USDA grant Project # 6050-21000-014-03S to KBH and JAO. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 25 TC 0 Z9 0 U1 4 U2 4 PU PEERJ INC PI LONDON PA 341-345 OLD ST, THIRD FLR, LONDON, EC1V 9LL, ENGLAND SN 2167-8359 J9 PEERJ JI PeerJ PD JUN 30 PY 2016 VL 4 AR e2155 DI 10.7717/peerj.2155 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DP9DR UT WOS:000378797400003 PM 27413635 ER PT J AU Migicovsky, Z Sawler, J Money, D Eibach, R Miller, AJ Luby, JJ Jamieson, AR Velasco, D von Kintzel, S Warner, J Wuhrer, W Brown, PJ Myles, S AF Migicovsky, Zoe Sawler, Jason Money, Daniel Eibach, Rudolph Miller, Allison J. Luby, James J. Jamieson, Andrew R. Velasco, Dianne von Kintzel, Sven Warner, John Wuhrer, Walter Brown, Patrick J. Myles, Sean TI Genomic ancestry estimation quantifies use of wild species in grape breeding SO BMC GENOMICS LA English DT Article ID VITIS-VINIFERA; GENETIC DIVERSITY; PIERCES DISEASE; FUTURE; WINE; DOMESTICATION; ASSOCIATION; INDUSTRY; ORIGINS; HYBRIDS AB Background: Grapes are one of the world's most valuable crops and most are made into wine. Grapes belong to the genus Vitis, which includes over 60 inter-fertile species. The most common grape cultivars derive their entire ancestry from the species Vitis vinifera, but wild relatives have also been exploited to create hybrid cultivars, often with increased disease resistance. Results: We evaluate the genetic ancestry of some of the most widely grown commercial hybrids from North America and Europe. Using genotyping-by-sequencing (GBS), we generated 2482 SNPs and 56 indels from 7 wild Vitis, 7 V. vinifera, and 64 hybrid cultivars. We used a principal component analysis (PCA) based ancestry estimation procedure and verified its accuracy with both empirical and simulated data. V. vinifera ancestry ranged from 11 % to 76 % across hybrids studied. Approximately one third (22/64) of the hybrids have ancestry estimates consistent with F1 hybridization: they derive half of their ancestry from wild Vitis and half from V. vinifera. Conclusions: Our results suggest that hybrid grape breeding is in its infancy. The distribution of V. vinifera ancestry across hybrids also suggests that backcrosses to wild Vitis species have been more frequent than backcrosses to V. vinifera during hybrid grape breeding. This pattern is unusual in crop breeding, as it is most common to repeatedly backcross to elite, or domesticated, germplasm. We anticipate our method can be extended to facilitate marker-assisted selection in order to introgress beneficial wild Vitis traits, while allowing for offspring with the highest V. vinifera content to be selected at the seedling stage. C1 [Migicovsky, Zoe; Sawler, Jason; Money, Daniel; Myles, Sean] Dalhousie Univ, Fac Agr, Dept Plant & Anim Sci, Truro, NS, Canada. [Sawler, Jason] Anandia Labs, 2259 Lower Mall, Vancouver, BC, Canada. [Eibach, Rudolph] JKI Inst Grapevine Breeding, Geilweilerhof, Germany. [Miller, Allison J.] St Louis Univ, Dept Biol, St Louis, MO 63103 USA. [Luby, James J.] Univ Minnesota, Dept Hort Sci, St Paul, MN 55108 USA. [Jamieson, Andrew R.] Agr & Agri Food Canada, Atlantic Food & Hort Res Ctr, Kentville, NS, Canada. [Velasco, Dianne] USDA ARS, Natl Clonal Germplasm Rep, Davis, CA USA. [von Kintzel, Sven] Jost Vineyards, 48 Vintage Lane, Malagash, NS, Canada. [Warner, John] Warner Vineyards, 391 Thorpe Rd,RR 2, Centreville, NS, Canada. [Wuhrer, Walter] Wuhrer Vineyards, 187 Highway 221,RR 1, Kingston, NS, Canada. [Brown, Patrick J.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. RP Myles, S (reprint author), Dalhousie Univ, Fac Agr, Dept Plant & Anim Sci, Truro, NS, Canada. EM sean.myles@dal.ca OI Migicovsky, Zoe/0000-0002-3931-1258 FU Canada Research Chairs program; National Sciences and Engineering Research Council of Canada; Genome Canada; Killam Predoctoral Scholarship from Dalhousie University FX This article was written, in part, thanks to funding from the Canada Research Chairs program, the National Sciences and Engineering Research Council of Canada and Genome Canada. Z.M. was supported in part by a Killam Predoctoral Scholarship from Dalhousie University. NR 48 TC 1 Z9 1 U1 8 U2 12 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUN 30 PY 2016 VL 17 AR 478 DI 10.1186/s12864-016-2834-8 PG 8 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DQ0MS UT WOS:000378894000001 PM 27357509 ER PT J AU Sang, YM Liu, QF Lee, J Ma, WJ McVey, DS Blecha, F AF Sang, Yongming Liu, Qinfang Lee, Jinhwa Ma, Wenjun McVey, D. Scott Blecha, Frank TI Expansion of amphibian intronless interferons revises the paradigm for interferon evolution and functional diversity SO SCIENTIFIC REPORTS LA English DT Article ID I INTERFERONS; VIRAL-INFECTION; XENOPUS-LAEVIS; SYSTEM; CELLS; FROG; SUSCEPTIBILITY; IDENTIFICATION; PATHOGENICITY; POLYPLOIDY AB Interferons (IFNs) are key cytokines identified in vertebrates and evolutionary dominance of intronless IFN genes in amniotes is a signature event in IFN evolution. For the first time, we show that the emergence and expansion of intronless IFN genes is evident in amphibians, shown by 24-37 intronless IFN genes in each frog species. Amphibian IFNs represent a molecular complex more complicated than those in other vertebrate species, which revises the established model of IFN evolution to facilitate re-inspection of IFN molecular and functional diversity. We identified these intronless amphibian IFNs and their intron-containing progenitors, and functionally characterized constitutive and inductive expression and antimicrobial roles in infections caused by zoonotic pathogens, such as influenza viruses and Listeria monocytogenes. Amphibians, therefore, may serve as overlooked vectors/hosts for zoonotic pathogens, and the amphibian IFN system provides a model to study IFN evolution in molecular and functional diversity in coping with dramatic environmental changes during terrestrial adaption. C1 [Sang, Yongming; Liu, Qinfang; Blecha, Frank] Kansas State Univ, Coll Vet Med, Dept Anat & Physiol, Manhattan, KS 66506 USA. [Lee, Jinhwa; Ma, Wenjun; McVey, D. Scott] Kansas State Univ, Coll Vet Med, Dept Diagnost Med & Pathobiol, Manhattan, KS 66506 USA. [Ma, Wenjun; McVey, D. Scott] ARS, Arthropod Borne Anim Dis Res Unit, Ctr Grain & Anim Hlth Res, USDA, Manhattan, KS USA. RP Sang, YM; Blecha, F (reprint author), Kansas State Univ, Coll Vet Med, Dept Anat & Physiol, Manhattan, KS 66506 USA.; Ma, WJ (reprint author), Kansas State Univ, Coll Vet Med, Dept Diagnost Med & Pathobiol, Manhattan, KS 66506 USA.; Ma, WJ (reprint author), ARS, Arthropod Borne Anim Dis Res Unit, Ctr Grain & Anim Hlth Res, USDA, Manhattan, KS USA. EM ysang@vet.k-state.edu; wma@vet.k-state.edu; Blecha@vet.k-state.edu FU Faculty Development Fund, KSU; Start-up Fund, KSU; USDA NIFA AFRI [2013-67015-21236, 2015-67015-23216] FX This work was mainly supported by the Faculty Development Fund, KSU to Y.S., and Start-up Fund, KSU to W.M.; and in part by USDA NIFA AFRI 2013-67015-21236 and USDA NIFA AFRI 2015-67015-23216. We thank Dr. Barbara Lutjemeier and Ms. Jessica Pearson for their excellent technical support. This is contribution number 16-215-J from the Kansas Agricultural Experiment Station. NR 53 TC 5 Z9 5 U1 1 U2 5 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUN 30 PY 2016 VL 6 AR 29072 DI 10.1038/srep29072 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DP9GD UT WOS:000378803800001 PM 27356970 ER PT J AU Vatanparast, M Shetty, P Chopra, R Doyle, JJ Sathyanarayana, N Egan, AN AF Vatanparast, Mohammad Shetty, Prateek Chopra, Ratan Doyle, Jeff J. Sathyanarayana, N. Egan, Ashley N. TI Transcriptome sequencing and marker development in winged bean (Psophocarpus tetragonolobus; Leguminosae) SO SCIENTIFIC REPORTS LA English DT Article ID TRYPSIN-INHIBITOR FAMILY; CICER-ARIETINUM L.; L DC; MEDICAGO-TRUNCATULA; GENOMICS RESEARCH; GENETIC-MARKERS; ORPHAN LEGUME; SNP DISCOVERY; EST-SSRS; RNA-SEQ AB Winged bean, Psophocarpus tetragonolobus (L.) DC., is similar to soybean in yield and nutritional value but more viable in tropical conditions. Here, we strengthen genetic resources for this orphan crop by producing a de novo transcriptome assembly and annotation of two Sri Lankan accessions (denoted herein as CPP34 [PI 491423] and CPP37 [PI 639033]), developing simple sequence repeat (SSR) markers, and identifying single nucleotide polymorphisms (SNPs) between geographically separated genotypes. A combined assembly based on 804,757 reads from two accessions produced 16,115 contigs with an N50 of 889 bp, over 90% of which has significant sequence similarity to other legumes. Combining contigs with singletons produced 97,241 transcripts. We identified 12,956 SSRs, including 2,594 repeats for which primers were designed and 5,190 high-confidence SNPs between Sri Lankan and Nigerian genotypes. The transcriptomic data sets generated here provide new resources for gene discovery and marker development in this orphan crop, and will be vital for future plant breeding efforts. We also analyzed the soybean trypsin inhibitor (STI) gene family, important plant defense genes, in the context of related legumes and found evidence for radiation of the Kunitz trypsin inhibitor (KTI) gene family within winged bean. C1 [Vatanparast, Mohammad; Egan, Ashley N.] Smithsonian Inst NMNH, Dept Bot, US Natl Herbarium US, 10th & Constitut Ave, Washington, DC 20013 USA. [Shetty, Prateek] Michigan State Univ, Dept Plant Biol, 612 Wilson Rd,Room 166, E Lansing, MI 48824 USA. [Chopra, Ratan] USDA ARS, 3810 4th St, Lubbock, TX 79415 USA. [Doyle, Jeff J.] Cornell Univ, Sch Integrat Plant Sci, Sect Plant Breeding & Genet, 412 Mann Lib, Ithaca, NY 14853 USA. [Sathyanarayana, N.] Sikkim Univ, Dept Bot, 5th Mile, Gangtok 737102, Sikkim, India. RP Egan, AN (reprint author), Smithsonian Inst NMNH, Dept Bot, US Natl Herbarium US, 10th & Constitut Ave, Washington, DC 20013 USA.; Sathyanarayana, N (reprint author), Sikkim Univ, Dept Bot, 5th Mile, Gangtok 737102, Sikkim, India. EM nsathyanarayana@cus.ac.in; egana@si.edu OI CHOPRA, RATAN/0000-0003-2088-3341 FU US National Science Foundation [DEB-0948800]; ANE [DEB-1352217] FX Thanks go to Sue Sherman-Broyles and Jane L. Doyle for help in the greenhouse. Thanks to Matthew Kweskin and Vanessa Gonzalez from the Smithsonian National Museum of Natural History for help with data analysis. Computations were completed on the Smithsonian Institution High Performance Cluster (SI/HPC). This research was supported by grants from the US National Science Foundation to JJD (DEB-0948800) and ANE (DEB-1352217). We acknowledge the support of Sir M Visvesvaraya Institute of Technology (Sir MVIT), Bangalore and Sikkim University, Gangtok for facilities. NR 85 TC 0 Z9 0 U1 14 U2 24 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUN 30 PY 2016 VL 6 DI 10.1038/srep29070 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DP8VL UT WOS:000378775900001 PM 27356763 ER PT J AU Dvorak, CMT Yang, Y Haley, C Sharma, N Murtaugh, MP AF Dvorak, Cheryl M. T. Yang, Yan Haley, Charles Sharma, Nikita Murtaugh, Michael P. TI National reduction in porcine circovirus type 2 prevalence following introduction of vaccination SO VETERINARY MICROBIOLOGY LA English DT Article DE Porcine circovirus 2; Swine; PCV2; Vaccination; Veterinary virology; Epidemiology; Immunology ID MULTISYSTEMIC WASTING SYNDROME; REAL-TIME PCR; INDUCED IMMUNITY; PCV2; PIGS; INFECTION; GENOTYPES; TRANSMISSION; PATHOGENESIS; RESPONSES AB Porcine circovirus type 2 (PCV2), a small, single-stranded circular DNA virus and the causative agent of porcine circovirus associated disease (PCVAD), was first observed in the mid-1990s in pigs with a post-weaning wasting disease. In 2006 the number of PCVAD cases greatly increased, marking it as an important viral pathogen for the United States (US) swine industry. PCV2 vaccines were introduced to the US in 2006 in response to widespread outbreaks of PCVAD. These vaccines were effective in preventing disease, but did not eliminate virus from the animals. In 2006, prior to vaccine use, a study of PCV2 prevalence in pig herds across the US was performed in conjunction with the US National Animal Health Monitoring System. In 2012, 6 years after widespread PCV2 vaccination, this study was repeated. Since the introduction of PCV2 vaccines in 2006, viral presence and viral loads have greatly decreased, and a genotypic shift dominated by PCV2b has occurred. Antibody levels have decreased in the pig population, but approximately 95% of sites continue to be antibody-positive. Widespread vaccination has controlled PCVAD and decreased PCV2 prevalence to the point that viremia is not detected on many sites. Thus, continued vaccination may lead to PCV2 elimination in the national herd over time. (C) 2016 Elsevier B.V. All rights reserved. C1 [Dvorak, Cheryl M. T.; Yang, Yan; Sharma, Nikita; Murtaugh, Michael P.] Univ Minnesota, Dept Vet & Biomed Sci, 1971 Commonwealth Ave, St Paul, MN 55108 USA. [Haley, Charles] USDA APHIS VS, Ctr Epidemiol & Anim Hlth, 2150 Ctr Ave,Bldg B,MS 2E7, Ft Collins, CO USA. RP Dvorak, CMT (reprint author), Univ Minnesota, Dept Vet & Biomed Sci, 1971 Commonwealth Ave, St Paul, MN 55108 USA. EM dvora013@umn.edu; yyan14@gmail.com; charles.a.haley@aphis.usda.gov; sharm131@umn.edu; murta001@umn.edu FU USDA CSRS project [MIN-63-112] FX The authors thank the USDA APHIS personnel, swine producers, and veterinarians whose efforts made this study possible. The research was supported in part by USDA CSRS project number MIN-63-112. NR 22 TC 4 Z9 4 U1 5 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 EI 1873-2542 J9 VET MICROBIOL JI Vet. Microbiol. PD JUN 30 PY 2016 VL 189 BP 86 EP 90 DI 10.1016/j.vetmic.2016.05.002 PG 5 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA DP4FE UT WOS:000378450900012 PM 27259831 ER PT J AU Mound, L Cavalleri, A O'Donnell, C Infante, F Ortiz, A Goldarazena, A AF Mound, Laurence Cavalleri, Adriano O'Donnell, Cheryle Infante, Francisco Ortiz, Antonio Goldarazena, Arturo TI Ambaeolothrips: a new genus of Neotropical Aeolothripidae (Thysanoptera), with observations on the type-species from mango trees in Mexico SO ZOOTAXA LA English DT Article DE new genus; new species; generic classification; character-state variation; predation AB Ambaeolothrips gen. n. is diagnosed for three Neotropical species: the type species romanruizi Ruiz-De la Cruz et al. comb. n. from Mexico, microstriatus Hood comb. n. from Panama, and pampeanus sp. n. from southern Brazil. Variation is discussed among character states that are used in the generic classification of the family Aeolothripidae, including segmentation of the antennae and maxillary palps, sculpture of the metanotum and presence of sternal discal setae. New field observations on the biology of romanruizi indicate that this species is phytophagous in flowers and on leaves, with no evidence of predation on the larvae of other thrips. C1 [Mound, Laurence] CSIRO, Australian Natl Insect Collect, Canberra, ACT, Australia. [Cavalleri, Adriano] Univ Fed Rio Grande, Sao Lourenco Do Sul, Brazil. [O'Donnell, Cheryle] USDA APHIS PPQ NIS, Beltsville, MD USA. [Infante, Francisco; Ortiz, Antonio] El Colegio Frontera Sur ECOSUR, Tapachula, Chiapas, Mexico. [Goldarazena, Arturo] Catholic Univ Louvain, ELIB, Louvain La Neuve, Belgium. RP Mound, L (reprint author), CSIRO, Australian Natl Insect Collect, Canberra, ACT, Australia. EM laurence.mound@csiro.au; cavalleri_adriano@yahoo.com.br; Cheryle.A.ODonnell@aphis.usda.gov; finfante@ecosur.mx; arturo.goldaracena@uclouvain.be RI Cavalleri, Adriano/D-3264-2015; Gesseff, Ednilson/A-3019-2017; Mound, Laurence/A-3967-2009; OI Cavalleri, Adriano/0000-0003-4163-6745; Infante, Francisco/0000-0002-7419-7606 FU El Consejo Nacional de Ciencia y Tecnologia (CONACYT) from Mexico FX The authors thank the El Consejo Nacional de Ciencia y Tecnologia (CONACYT) from Mexico for grant funding to F. Infante, and the U.S. National Museum of Natural History for providing access to type material. We are also grateful for help and advice we received from the editor, David Ouvrard, and two anonymous reviewers. NR 13 TC 0 Z9 0 U1 0 U2 0 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD JUN 30 PY 2016 VL 4132 IS 3 BP 413 EP 421 DI 10.11646/zootaxa.4132.3.9 PG 9 WC Zoology SC Zoology GA DP9XU UT WOS:000378852100009 PM 27395682 ER PT J AU Lopez-Uribe, MM Cane, JH Minckley, RL Danforth, BN AF Lopez-Uribe, Margarita M. Cane, James H. Minckley, Robert L. Danforth, Bryan N. TI Crop domestication facilitated rapid geographical expansion of a specialist pollinator, the squash bee Peponapis pruinosa SO PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Article DE Holocene; Cucurbita spp.; approximate Bayesian computation; demographic inference ID EASTERN NORTH-AMERICA; GIANT RESIN BEE; RANGE EXPANSION; CUCURBITA-PEPO; HONEY-BEE; PHYLOGENETIC-RELATIONSHIPS; HYMENOPTERA-MEGACHILIDAE; GENETIC CONSEQUENCES; MICROSATELLITE DATA; POPULATION HISTORY AB Squash was first domesticated in Mexico and is now found throughout North America (NA) along with Peponapis pruinosa, a pollen specialist bee species of the squash genus Cucurbita. The origin and spread of squash cultivation is well-studied archaeologically and phylogenetically; however, no study has documented how cultivation of this or any other crop has influenced species in mutualistic interactions. We used molecular markers to reconstruct the demographic range expansion and colonization routes of P. pruinosa from its native range into temperate NA. Populations east of the Rocky Mountains expanded from the wild host plant's range in Mexico and were established by a series of founder events. Eastern North America was most likely colonized from squash bee populations in the present-day continental Midwest USA and not from routes that followed the Gulf and Atlantic coasts from Mexico. Populations of P. pruinosa west of the Rockies spread north from the warm deserts much more recently, showing two genetically differentiated populations with no admixture: one in California and the other one in eastern Great Basin. These bees have repeatedly endured severe bottlenecks as they colonized NA, following human spread of their Cucurbita pollen hosts during the Holocene. C1 [Lopez-Uribe, Margarita M.; Danforth, Bryan N.] Cornell Univ, Dept Entomol, Ithaca, NY 14850 USA. [Lopez-Uribe, Margarita M.] North Carolina State Univ, Dept Entomol & Plant Pathol, Raleigh, NC 27695 USA. [Lopez-Uribe, Margarita M.] North Carolina State Univ, Dept Appl Ecol, Raleigh, NC 27695 USA. [Cane, James H.] Utah State Univ, USDA ARS, Bee Biol & Systemat Lab, Logan, UT 84322 USA. [Minckley, Robert L.] Univ Rochester, Dept Biol, Rochester, NY 14627 USA. RP Lopez-Uribe, MM (reprint author), Cornell Univ, Dept Entomol, Ithaca, NY 14850 USA.; Lopez-Uribe, MM (reprint author), North Carolina State Univ, Dept Entomol & Plant Pathol, Raleigh, NC 27695 USA.; Lopez-Uribe, MM (reprint author), North Carolina State Univ, Dept Appl Ecol, Raleigh, NC 27695 USA. EM mmlopezu@ncsu.edu FU Grace Griswold Endowment (Cornell University); National Science Foundation [DEB-0814544, DEB-0742998] FX Funding for this study was provided by grants from the Grace Griswold Endowment (Cornell University to M.M.L.U.), and by awards from the National Science Foundation (DEB-0814544 and DEB-0742998 to B.N.D.). NR 67 TC 0 Z9 0 U1 10 U2 10 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8452 EI 1471-2954 J9 P ROY SOC B-BIOL SCI JI Proc. R. Soc. B-Biol. Sci. PD JUN 29 PY 2016 VL 283 IS 1833 AR 20160443 DI 10.1098/rspb.2016.0443 PG 9 WC Biology; Ecology; Evolutionary Biology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Evolutionary Biology GA DT1MR UT WOS:000381247300020 ER PT J AU Potter, TL Bosch, DD Strickland, TC AF Potter, Thomas L. Bosch, David D. Strickland, Timothy C. TI Field and Laboratory Dissipation of the Herbicide Fomesafen in the Southern Atlantic Coastal Plain (USA) SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE pesticide transport; conservation tillage; strip tillage; conventional tillage; leaching metabolites ID AMARANTH AMARANTHUS-PALMERI; RUNOFF; TILLAGE; PESTICIDES; GEORGIA; IMPACT; WATER; DEGRADATION; TRANSPORT; RESIDUE AB To control weeds with evolved resistance to glyphosate, Southeastern (USA) cotton farmers have increased fomesafen (5-(2-chloro-alpha,alpha,alpha-trifluoro-p-tolyloxy)-N-mesy1-2-nitrobenzamide) use. To refine fomesafen risk assessments, data are needed that describe its dissipation following application to farm fields. In our study, relatively low runoff rates and transport by lateral subsurface flow, <1.0 and 0.15% of applied respectively, were observed. The low runoff rate was linked to postapplication irrigation incorporation and implementation of a common conservation tillage practice. Moderate soil persistence (t(1/2) = 100 days) was indicated in laboratory incubations with surface soil, however, analysis of soil cores from treated plots showed that approximate to 3% of fomesafen applied persisted in subsoil >3 years after application. Findings suggest low potential for fomesafen movement from treated fields, however, the fate of fomesafen that accumulated in subsoil and the identity of degradates are uncertain. Soil and water samples were screened for degradates, but, none were detected. C1 [Potter, Thomas L.; Bosch, David D.; Strickland, Timothy C.] USDA ARS, Southeast Watershed Res Lab, POB 748, Tifton, GA 31793 USA. RP Potter, TL (reprint author), USDA ARS, Southeast Watershed Res Lab, POB 748, Tifton, GA 31793 USA. EM Tom.Potter@ars.usda.gov FU USDA-Agricultural Research Service; University of Georgia Coastal Plain Experiment Station FX The USDA-Agricultural Research Service and University of Georgia Coastal Plain Experiment Station supported this work. NR 40 TC 0 Z9 0 U1 7 U2 12 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 29 PY 2016 VL 64 IS 25 BP 5156 EP 5163 DI 10.1021/acs.jafc.6b01649 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DQ1TY UT WOS:000378984500003 PM 27268304 ER PT J AU Zhang, BC Luo, YG Kanyuck, K Bauchan, G Mowery, J Zavalij, P AF Zhang, Boce Luo, Yaguang Kanyuck, Kelsey Bauchan, Gary Mowery, Joseph Zavalij, Peter TI Development of Metal-Organic Framework for Gaseous Plant Hormone Encapsulation To Manage Ripening of Climacteric Produce SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE metal-organic framework; ethylene; climacteric produce; food waste ID CARBON-DIOXIDE; BANANA FRUIT; ETHYLENE; ZEOLITE; ADSORPTION; ETHEPHON; METHANE; ETHANE; 1-MCP; ACID AB Controlled ripening of climacteric fruits, such as bananas and avocados, is a critical step to provide consumers with high-quality products while reducing postharvest losses. Prior to ripening, these fruits can be stored for an extended period of time but are usually not suitable for consumption. However, once ripening is initiated, they undergo irreversible changes that lead to rapid quality loss and decay if not consumed within a short window of time. Therefore, technologies to slow the ripening process after its onset or to stimulate ripening immediately before consumption are in high demand. In this study, we developed a solid porous metal organic framework (MOP) to encapsulate gaseous ethylene for subsequent release. We evaluated the feasibility of this technology for on-demand stimulated ripening of bananas and avocados. Copper terephthalate (CuTPA) MOF was synthesized via a solvothermal method and loaded with ethylene gas. Its crystalline structure and chemical composition were characterized by X-ray diffraction crystallography, porosity by N-2 and ethylene isotherms, and morphology by electron microscopy. The MOF loaded with ethylene (MOF ethylene) was placed inside sealed containers with preclimacteric bananas and avocados and stored at 16 degrees C. The headspace gas composition and fruit color and texture were monitored periodically. Results showed that this CuTPA MOF is highly porous, with a total pore volume of 0.39 cm(3)/g. A 50 mg portion of MOF ethylene can absorb and release up to 654 mu L/L of ethylene in a 4 L container. MOF ethylene significantly accelerated the ripening-related color and firmness changes of treated bananas and avocados. This result suggests that MOF ethylene technology could be used for postharvest application to stimulate ripening just before the point of consumption. C1 [Zhang, Boce; Luo, Yaguang] ARS, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. [Zhang, Boce; Luo, Yaguang] ARS, Food Qual Lab, Beltsville, MD 20705 USA. [Bauchan, Gary; Mowery, Joseph] ARS, Electron & Confocal Microscope Unit, USDA, Beltsville, MD 20705 USA. [Kanyuck, Kelsey] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Zavalij, Peter] Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA. RP Luo, YG (reprint author), ARS, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA.; Luo, YG (reprint author), ARS, Food Qual Lab, Beltsville, MD 20705 USA. EM yaguang.luo@ars.usda.gov FU USDA-NIFA Specialty Crop Research Initiative Grant [MDW-2010-01165] FX This work was supported by USDA-NIFA Specialty Crop Research Initiative Grant Award No. MDW-2010-01165. The authors acknowledge the support of Dr. John Zielinski at Intertek Chemicals and Pharmaceuticals service to conduct the work of N2/ethylene isotherms, BET, and TOPV measurements. The mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture (USDA). The USDA is an equal opportunity provider and employer. NR 34 TC 0 Z9 0 U1 14 U2 20 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 29 PY 2016 VL 64 IS 25 BP 5164 EP 5170 DI 10.1021/acs.jafc.6b02072 PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DQ1TY UT WOS:000378984500004 PM 27250565 ER PT J AU Lee, S Yeo, IY Sadeghi, AM McCarty, GW Hively, WD Lang, MW AF Lee, Sangchul Yeo, In-Young Sadeghi, Ali M. McCarty, Gregory W. Hively, W. Dean Lang, Megan W. TI Impacts of Watershed Characteristics and Crop Rotations on Winter Cover Crop Nitrate-Nitrogen Uptake Capacity within Agricultural Watersheds in the Chesapeake Bay Region SO PLOS ONE LA English DT Article ID NUTRIENT-UPTAKE EFFICIENCY; MODEL CALIBRATION; UNITED-STATES; SWAT MODEL; QUALITY; SIMULATION; SOIL; MINERALIZATION; RESIDUES; CORN AB The adoption rate of winter cover crops (WCCs) as an effective conservation management practice to help reduce agricultural nutrient loads in the Chesapeake Bay (CB) is increasing. However, the WCC potential for water quality improvement has not been fully realized at the watershed scale. This study was conducted to evaluate the long-term impact of WCCs on hydrology and NO3-N loads in two adjacent watersheds and to identify key management factors that affect the effectiveness of WCCs using the Soil and Water Assessment Tool (SWAT) and statistical methods. Simulation results indicated that WCCs are effective for reducing NO3-N loads and their performance varied based on planting date, species, soil characteristics, and crop rotations. Early-planted WCCs outperformed late-planted WCCs on the reduction of NO3-N loads and early-planted rye (RE) reduced NO3-N loads by similar to 49.3% compared to the baseline (no WCC). The WCCs were more effective in a watershed dominated by well-drained soils with increased reductions in NO3-N fluxes of similar to 2.5 kg N.ha(-1) delivered to streams and similar to 10.1 kg N.ha(-1) leached into groundwater compared to poorly-drained soils. Well-drained agricultural lands had higher transport of NO3-N in the soil profile and groundwater due to increased N leaching. Poorly-drained agricultural lands had lower NO3-N due to extensive drainage ditches and anaerobic soil conditions promoting denitrification. The performance of WCCs varied by crop rotations (i.e., continuous corn and corn-soybean), with increased N uptake following soybean crops due to the increased soil mineral N availability by mineralization of soybean residue compared to corn residue. The WCCs can reduce N leaching where baseline NO3-N loads are high in well-drained soils and/or when residual and mineralized N availability is high due to the cropping practices. The findings suggested that WCC implementation plans should be established in watersheds according to local edaphic and agronomic characteristics for reducing N leaching. C1 [Lee, Sangchul; Yeo, In-Young; Lang, Megan W.] Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. [Yeo, In-Young] Univ Newcastle, Sch Engn, Callaghan, NSW, Australia. [Sadeghi, Ali M.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD USA. [McCarty, Gregory W.; Hively, W. Dean] USGS, Eastern Geog Sci Ctr, Reston, VA USA. RP Yeo, IY (reprint author), Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA.; Yeo, IY (reprint author), Univ Newcastle, Sch Engn, Callaghan, NSW, Australia. EM In-Young.Yeo@newcastle.edu.au FU National Aeronautics and Space Administration Land Cover and Land Use Change Program; United States Department of Agriculture Conservation Effects Assessment Project; U.S. Geological Survey Land Change Science Program; UMD Libraries Open Access Publishing Fund FX This research was supported by National Aeronautics and Space Administration Land Cover and Land Use Change Program (http://lcluc.umd.edu/), United States Department of Agriculture Conservation Effects Assessment Project (http://www.nrcs.usda.gov/wps/portal/nrcs/main/national/technical/nra/ce ap/), U.S. Geological Survey Land Change Science Program (http://www.usgs.gov/climate_landuse/lcs/), and the UMD Libraries Open Access Publishing Fund (http://www.lib.umd.edu/oa/openaccessfund). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 40 TC 1 Z9 1 U1 14 U2 21 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 28 PY 2016 VL 11 IS 6 AR e0157637 DI 10.1371/journal.pone.0157637 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DQ0AH UT WOS:000378858900012 PM 27352119 ER PT J AU Schroeder, H Cronn, R Yanbaev, Y Jennings, T Mader, M Degen, B Kersten, B AF Schroeder, Hilke Cronn, Richard Yanbaev, Yulai Jennings, Tara Mader, Malte Degen, Bernd Kersten, Birgit TI Development of Molecular Markers for Determining Continental Origin of Wood from White Oaks (Quercus L. sect. Quercus) SO PLOS ONE LA English DT Article ID CHLOROPLAST DNA VARIATION; LAND PLANTS; POSTGLACIAL COLONIZATION; PHYLOGENETIC ANALYSIS; IDENTIFICATION; DIFFERENTIATION; SYSTEMATICS; BARCODE; PHYLOGEOGRAPHY; BIOGEOGRAPHY AB To detect and avoid illegal logging of valuable tree species, identification methods for the origin of timber are necessary. We used next-generation sequencing to identify chloroplast genome regions that differentiate the origin of white oaks from the three continents; Asia, Europe, and North America. By using the chloroplast genome of Asian Q. mongolica as a reference, we identified 861 variant sites (672 single nucleotide polymorphisms (SNPs); 189 insertion/deletion (indel) polymorphism) from representative species of three continents (Q. mongolica from Asia; Q. petraea and Q. robur from Europe; Q. alba from North America), and we identified additional chloroplast polymorphisms in pools of 20 individuals each from Q. mongolica (789 variant sites) and Q. robur (346 variant sites). Genome sequences were screened for indels to develop markers that identify continental origin of oak species, and that can be easily evaluated using a variety of detection methods. We identified five indels and one SNP that reliably identify continent-of-origin, based on evaluations of up to 1078 individuals representing 13 white oak species and three continents. Due to the size of length polymorphisms revealed, this marker set can be visualized using capillary electrophoresis or high resolution gel (acrylamide or agarose) electrophoresis. With these markers, we provide the wood trading market with an instrument to comply with the U.S. and European laws that require timber companies to avoid the trade of illegally harvested timber. C1 [Schroeder, Hilke; Mader, Malte; Degen, Bernd; Kersten, Birgit] Thuenen Insitute Forest Genet, Grosshansdorf, Germany. [Cronn, Richard] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Yanbaev, Yulai] Bashkir State Univ, Ufa 450074, Russia. [Jennings, Tara] Oregon State Univ, Bot & Plant Pathol, Corvallis, OR 97331 USA. RP Schroeder, H (reprint author), Thuenen Insitute Forest Genet, Grosshansdorf, Germany. EM hilke.schroeder@thuenen.de RI Degen, Bernd/S-1730-2016; OI Cronn, Richard/0000-0001-5342-3494; Kersten, Birgit/0000-0001-9900-9133 FU German Federal Ministry of Food and Agriculture (BMEL); Deutsche Bundesstiftung Umwelt (DBU); USDA Forest Service International Programs Office; US Agency for International Development; US Department of State FX This project has been financially supported by German Federal Ministry of Food and Agriculture (BMEL) (http://www.bmel.de), the Deutsche Bundesstiftung Umwelt (DBU) (http://www.dbu.de), the USDA Forest Service International Programs Office (http://www.fs.fed.us/about-agency/international-programs), the US Agency for International Development, and US Department of State (http://www.state.gov/s/d/rm/rls/dosstrat/). The funders had no rule in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 58 TC 0 Z9 0 U1 23 U2 30 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 28 PY 2016 VL 11 IS 6 AR e0158221 DI 10.1371/journal.pone.0158221 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DQ0AH UT WOS:000378858900046 PM 27352242 ER PT J AU Wang, LS Kim, C Xu, X Piskurewicz, U Dogra, V Singh, S Mahler, H Apel, K AF Wang, Liangsheng Kim, Chanhong Xu, Xia Piskurewicz, Urszula Dogra, Vivek Singh, Somesh Mahler, Hanno Apel, Klaus TI Singlet oxygen- and EXECUTER1-mediated signaling is initiated in grana margins and depends on the protease FtsH2 SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE singlet oxygen; photosystem II; EXECUTER1; dependent signaling; stress; thylakoid FtsH protease ID PHOTOSYSTEM-II COMPLEX; ARABIDOPSIS-THALIANA; PROTOCHLOROPHYLLIDE OXIDOREDUCTASE; CHLOROPHYLL BIOSYNTHESIS; STRESS RESPONSES; QUALITY-CONTROL; D1 DEGRADATION; IN-VIVO; REPAIR; LIGHT AB Formation of singlet oxygen (O-1(2)) has been implicated with damaging photosystem II (PSII) that needs to undergo continuous repair to maintain photosynthetic electron transport. In addition to its damaging effect, O-1(2) has also been shown to act as a signal that triggers stress acclimation and an enhanced stress resistance. A signaling role of O-1(2) was first documented in the fluorescent (flu) mutant of Arabidopsis. It strictly depends on the chloroplast protein EXECUTER1 (EX1) and happens under nonphotoinhibitory light conditions. Under severe light stress, signaling is initiated independently of EX1 by O-1(2) that is thought to be generated at the acceptor side of active PSII within the core of grana stacks. The results of the present study suggest a second source of O-1(2) formation in grana margins close to the site of chlorophyll synthesis where EX1 is localized and the disassembly of damaged and reassembly of active PSII take place. The initiation of O-1(2) signaling in grana margins depends on EX1 and the ATP-dependent zinc metalloprotease FtsH. As FtsH cleaves also the D1 protein during the disassembly of damaged PSII, EX1- and O-1(2)-mediated signaling seems to be not only spatially but also functionally associated with the repair of PSII. C1 [Wang, Liangsheng; Kim, Chanhong; Xu, Xia; Piskurewicz, Urszula; Apel, Klaus] Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Kim, Chanhong; Dogra, Vivek; Singh, Somesh] Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Ctr Excellence Mol Plant Sci, Shanghai 201602, Peoples R China. [Mahler, Hanno] Swiss Fed Inst Technol, CH-8092 Zurich, Switzerland. [Xu, Xia] Cornell Univ, Grape Genet Res Unit, USDA, ARS, Geneva, NY 14456 USA. [Piskurewicz, Urszula] Univ Geneva, Dept Plant Biol, CH-1211 Geneva, Switzerland. RP Apel, K (reprint author), Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. EM kha24@cornell.edu RI Dogra, vivek/D-9868-2011 OI Dogra, vivek/0000-0003-1853-8274 FU Boyce Thompson Institute; National Institutes of Health [R01-GM085036]; Swiss Federal Institute of Technology; Swiss National Science Foundation; Chinese Academy of Sciences; National Natural Science Foundation of China (NSFC) [31570264] FX We thank Drs. D. Leister, T. Nakagawa, W. Sakamoto, and S. Greiner for providing curt1abcd quadruple mutant seeds and CURT1A antibody, pGWB504 and pGWB510 destination vectors, seeds of the tFtsH2-GFP-expressing line, and antiserum against cytochrome f, respectively; G. Wang and Dr. Y.-M. She for help with mass spectrometry; Drs. B. Grimm and B. Hedtke for providing antisera against glutamyl-tRNA reductase, glutamate-1-semialdehyde aminotransferase, Mg2+-chelatase subunit I, and Mg2+-protoporphyrin IX methyl transferase; Drs. T. Kleine and G. Wanner for efforts to detect EX1 by scanning electron microscopy; Drs. F. Landgraf, Y. Kato, K. v. Wijk, and G. Friso for help and contributions during the initial phase of this work; and S. Puthiyaveetil for information concerning the isolation of grana margins. This research was supported by the Boyce Thompson Institute; National Institutes of Health Grant R01-GM085036; the Swiss Federal Institute of Technology and the Swiss National Science Foundation (K.A.); and the Chinese Academy of Sciences and National Natural Science Foundation of China (NSFC) Grant 31570264 (to C.K.). NR 45 TC 1 Z9 1 U1 3 U2 5 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JUN 28 PY 2016 VL 113 IS 26 BP E3792 EP E3800 DI 10.1073/pnas.1603562113 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DQ2LI UT WOS:000379033400026 PM 27303039 ER PT J AU Yu, LX Liu, XC Boge, W Liu, XP AF Yu, Long-Xi Liu, Xinchun Boge, William Liu, Xiang-Ping TI Genome-Wide Association Study Identifies Loci for Salt Tolerance during Germination in Autotetraploid Alfalfa (Medicago sativa L.) Using Genotyping-by-Sequencing SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE linkage disequilibrium; salt stress; germination; genotyping by sequencing; association mapping ID SEED-GERMINATION; ABIOTIC STRESSES; ABSCISIC-ACID; ARABIDOPSIS; SALINITY; TRUNCATULA; RESPONSES; DROUGHT; NACL; RESISTANCE AB Salinity is one of major abiotic stresses limiting alfalfa (Medicago sativa L.) production in the arid and semi-arid regions in US and other counties. In this study, we used a diverse panel of alfalfa accessions previously described by Zhang et al. (2015) to identify molecular markers associated with salt tolerance during germination using genome-wide association study (GWAS) and genotyping-by-sequencing (GBS). Phenotyping was done by germinating alfalfa seeds under different levels of salt stress. Phenotypic data of adjusted germination rates and SNP markers generated by GBS were used for marker-trait association. Thirty six markers were significantly associated with salt tolerance in at least one level of salt treatments. Alignment of sequence tags to the Medicago truncatula genome revealed genetic locations of the markers on all chromosomes except chromosome 3. Most significant markers were found on chromosomes 1, 2, and 4. BLAST search using the flanking sequences of significant markers identified 14 putative candidate genes linked to 23 significant markers. Most of them were repeatedly identified in two or three salt treatments. Several loci identified in the present study had similar genetic locations to the reported QTL associated with salt tolerance in M. truncatula. A locus identified on chromosome 6 by this study overlapped with that by drought in our previous study. To our knowledge, this is the first report on mapping loci associated with salt tolerance during germination in autotetraploid alfalfa. Further investigation on these loci and their linked genes would provide insight into understanding molecular mechanisms by which salt and drought stresses affect alfalfa growth. Functional markers closely linked to the resistance loci would be useful for MAS to improve alfalfa cultivars with enhanced resistance to drought and salt stresses. C1 [Yu, Long-Xi; Liu, Xinchun; Boge, William; Liu, Xiang-Ping] ARS, USDA, Plant Germplasm Intro Testing & Res, Prosser, WA USA. [Liu, Xinchun] Sichuan Agr Univ, Barley Res Ctr, Coll Agron, Chengdu, Peoples R China. RP Yu, LX (reprint author), ARS, USDA, Plant Germplasm Intro Testing & Res, Prosser, WA USA. EM longxi.yu@ars.usda.gov FU USDA-ARS National Program [5354-21000-015-00D]; USDA-NIFA AFRP fund [2015-70005-24071] FX This work was supported by USDA-ARS National Program Project No. 5354-21000-015-00D and USDA-NIFA AFRP fund (Award No. 2015-70005-24071). NR 45 TC 2 Z9 2 U1 23 U2 34 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JUN 28 PY 2016 VL 7 AR 956 DI 10.3389/fpls.2016.00956 PG 12 WC Plant Sciences SC Plant Sciences GA DP6JX UT WOS:000378604500001 PM 27446182 ER PT J AU Kandpal, LM Lohumi, S Kim, MS Kang, JS Cho, BK AF Kandpal, Lalit Mohan Lohumi, Santosh Kim, Moon S. Kang, Jum-Soon Cho, Byoung-Kwan TI Near-infrared hyperspectral imaging system coupled with multivariate methods to predict viability and vigor in muskmelon seeds SO SENSORS AND ACTUATORS B-CHEMICAL LA English DT Article DE Seed viability; Hyperspectral imaging; Partial least-squares discriminant analysis (PLSDA); Variable selection methods; Germination ability; Muskmelon seeds ID SELECTIVITY RATIO PLOT; PARTIAL LEAST-SQUARES; VARIABLE SELECTION; REFLECTANCE SPECTRA; DISCRIMINATION; REGRESSION; CHEMOMETRICS; SPECTROSCOPY; CALIBRATION; PROFILES AB A near-infrared (NIR) hyperspectral imaging (HSI) system was used to predict viability and vigor (in term of germination periods) in muskmelon seeds. Hyperspectral images of muskmelon seeds were acquired using a NIR push-broom HSI system covering the spectral range of 948-2494 nm. After NIR spectra collection, all seeds underwent a germination test to confirm their viability and vigor. The spectra from seeds with 3 and 5 germination days and nongerminated seeds were further used for development of a classification model of partial least-squares discriminant analysis (PLSDA). Most effective wavelengths were selected using three model-based variable selection methods, i.e., variable important in projection (VIP), selectivity ratio (SR), and significance multivariate correlation (sMC), which selected 23, 18, and 19 optimal variables, respectively, from full set of 208 variables. The selected variables from different waveband selection methods were found genuine and significant for interpreting the prediction results of seed viability and vigor. Subsequently, the PLS-DA model was constructed using individual VIP-, SR-, or sMC-selected variables. The results demonstrated that the PLSDA model developed with the selected optimal variables from the different methods provided comparable results for the calibration set; however, the PLSDA-SR method afforded the highest classification accuracy (94.6%) for a validation set used to determine the viability and vigor of muskmelon seeds. The wavelengths selected by the different methods represents chemical components of the seed and the attribute of germination ability was chosen most often. (c) 2016 Elsevier B.V. All rights reserved. C1 [Kandpal, Lalit Mohan; Lohumi, Santosh; Cho, Byoung-Kwan] Chungnam Natl Univ, Dept Biosyst Machinery Engn, Coll Agr & Life Sci, 99 Daehak Ro, Daejeon 305764, South Korea. [Kim, Moon S.] ARS, Environm Microbial & Food Safety Lab, USDA, BARC East, Powder Mill Rd,Bldg 303, Beltsville, MD 20705 USA. [Kang, Jum-Soon] Pusan Natl Univ, Dept Hort Biosci, Miryang 627706, South Korea. RP Cho, BK (reprint author), Chungnam Natl Univ, Dept Biosyst Machinery Engn, Coll Agr & Life Sci, 99 Daehak Ro, Daejeon 305764, South Korea. EM latitm85@gmail.com; Santosh123@cnu.ac.kr; moon.kim@ars.usda.gov; kangjs@pusan.ac.kr; chobk@cnu.ac.kr FU Golden Seed Project, Ministry of Agriculture, Food and Rural Affairs (MAFRA); Ministry of Oceans and Fisheries (MOF); Rural Development Administration (RDA); Korea Forest Service (KFS); Technology Commercialization Support Program, Ministry of Agriculture, Food and Rural Affairs (MAFRA), Republic of Korea FX This research was supported by Golden Seed Project, Ministry of Agriculture, Food and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries (MOF), Rural Development Administration (RDA) and Korea Forest Service (KFS) and by Technology Commercialization Support Program, Ministry of Agriculture, Food and Rural Affairs (MAFRA), Republic of Korea. NR 52 TC 4 Z9 4 U1 9 U2 36 PU ELSEVIER SCIENCE SA PI LAUSANNE PA PO BOX 564, 1001 LAUSANNE, SWITZERLAND SN 0925-4005 J9 SENSOR ACTUAT B-CHEM JI Sens. Actuator B-Chem. PD JUN 28 PY 2016 VL 229 BP 534 EP 544 DI 10.1016/j.snb.2016.02.015 PG 11 WC Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation SC Chemistry; Electrochemistry; Instruments & Instrumentation GA DH1DU UT WOS:000372525400064 ER PT J AU He, YP Ingudam, S Reed, S Gehring, A Strobaugh, TP Irwin, P AF He, Yiping Ingudam, Shakuntala Reed, Sue Gehring, Andrew Strobaugh, Terence P., Jr. Irwin, Peter TI Study on the mechanism of antibacterial action of magnesium oxide nanoparticles against foodborne pathogens SO JOURNAL OF NANOBIOTECHNOLOGY LA English DT Article DE MgO nanoparticles; Foodborne pathogens; Antimicrobial mechanism; H2O2; Oxidative stress ID OXIDATIVE STRESS RESISTANCE; CAMPYLOBACTER-JEJUNI; MGO NANOPARTICLES; ESCHERICHIA-COLI; LISTERIA-MONOCYTOGENES; HYDROGEN-PEROXIDE; IRON; TOXICITY; SURVIVAL; SPP. AB Background: Magnesium oxide nanoparticles (MgO nanoparticles, with average size of 20 nm) have considerable potential as antimicrobial agents in food safety applications due to their structure, surface properties, and stability. The aim of this work was to investigate the antibacterial effects and mechanism of action of MgO nanoparticles against several important foodborne pathogens. Results: Resazurin (a redox sensitive dye) microplate assay was used for measuring growth inhibition of bacteria treated with MgO nanoparticles. The minimal inhibitory concentrations of MgO nanoparticles to 10(4) colony-forming unit/ml (CFU/ml) of Campylobacter jejuni, Escherichia coli O157: H7, and Salmonella Enteritidis were determined to be 0.5, 1 and 1 mg/ml, respectively. To completely inactivate 10(8-9) CFU/ml bacterial cells in 4 h, a minimal concentration of 2 mg/ml MgO nanoparticles was required for C. jejuni whereas E. coli O157: H7 and Salmonella Enteritidis required at least 8 mg/ml nanoparticles. Scanning electron microscopy examination revealed clear morphological changes and membrane structural damage in the cells treated with MgO nanoparticles. A quantitative real-time PCR combined with ethidium monoazide pretreatment confirmed cell membrane permeability was increased after exposure to the nanoparticles. In a cell free assay, a low level (1.1 mu M) of H2O2 was detected in the nanoparticle suspensions. Consistently, MgO nanoparticles greatly induced the gene expression of KatA, a sole catalase in C. jejuni for breaking down H2O2 to H2O and O-2. Conclusions: MgO nanoparticles have strong antibacterial activity against three important foodborne pathogens. The interaction of nanoparticles with bacterial cells causes cell membrane leakage, induces oxidative stress, and ultimately leads to cell death. C1 [He, Yiping; Reed, Sue; Gehring, Andrew; Strobaugh, Terence P., Jr.; Irwin, Peter] ARS, Mol Characterizat Foodborne Pathogens Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Ingudam, Shakuntala] ICAR Res Complex NEH Reg, Umiam 793103, Meghalaya, India. RP He, YP (reprint author), ARS, Mol Characterizat Foodborne Pathogens Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM yiping.he@ars.usda.gov FU US Department of Agriculture, Agricultural Research Service (USDA-ARS); Department of Biotechnology, the Government of India Ministry of Science and Technology FX This research was supported by the US Department of Agriculture, Agricultural Research Service (USDA-ARS), and the overseas associateship award from the Department of Biotechnology, the Government of India Ministry of Science and Technology. NR 29 TC 1 Z9 1 U1 10 U2 20 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1477-3155 J9 J NANOBIOTECHNOL JI J. Nanobiotechnol. PD JUN 27 PY 2016 VL 14 AR 54 DI 10.1186/s12951-016-0202-0 PG 9 WC Biotechnology & Applied Microbiology; Nanoscience & Nanotechnology SC Biotechnology & Applied Microbiology; Science & Technology - Other Topics GA DQ3QV UT WOS:000379117900001 PM 27349516 ER PT J AU Livingston, KA Chung, M Sawicki, CM Lyle, BJ Wang, DD Roberts, SB McKeown, NM AF Livingston, Kara A. Chung, Mei Sawicki, Caleigh M. Lyle, Barbara J. Wang, Ding Ding Roberts, Susan B. McKeown, Nicola M. TI Development of a Publicly Available, Comprehensive Database of Fiber and Health Outcomes: Rationale and Methods SO PLOS ONE LA English DT Article ID DIETARY FIBER AB Background Dietary fiber is a broad category of compounds historically defined as partially or completely indigestible plant-based carbohydrates and lignin with, more recently, the additional criteria that fibers incorporated into foods as additives should demonstrate functional human health outcomes to receive a fiber classification. Thousands of research studies have been published examining fibers and health outcomes. Objectives (1) Develop a database listing studies testing fiber and physiological health outcomes identified by experts at the Ninth Vahouny Conference; (2) Use evidence mapping methodology to summarize this body of literature. This paper summarizes the rationale, methodology, and resulting database. The database will help both scientists and policy-makers to evaluate evidence linking specific fibers with physiological health outcomes, and identify missing information. Methods To build this database, we conducted a systematic literature search for human intervention studies published in English from 1946 to May 2015. Our search strategy included a broad definition of fiber search terms, as well as search terms for nine physiological health outcomes identified at the Ninth Vahouny Fiber Symposium. Abstracts were screened using a priori defined eligibility criteria and a low threshold for inclusion to minimize the likelihood of rejecting articles of interest. Publications then were reviewed in full text, applying additional a priori defined exclusion criteria. The database was built and published on the Systematic Review Data Repository (SRDR (TM)), a web-based, publicly available application. Conclusions A fiber database was created. This resource will reduce the unnecessary replication of effort in conducting systematic reviews by serving as both a central database archiving PICO ( population, intervention, comparator, outcome) data on published studies and as a searchable tool through which this data can be extracted and updated. C1 [Livingston, Kara A.; Sawicki, Caleigh M.; Roberts, Susan B.; McKeown, Nicola M.] Tufts Univ, USDA, Jean Mayer Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Chung, Mei; Wang, Ding Ding] Tufts Univ, Sch Med, Dept Publ Hlth & Community Med, Nutr Infect Unit, Boston, MA 02111 USA. [Lyle, Barbara J.] Int Life Sci Inst, North Amer Branch, Washington, DC USA. [McKeown, Nicola M.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. RP McKeown, NM (reprint author), Tufts Univ, USDA, Jean Mayer Human Nutr Res Ctr Aging, Boston, MA 02111 USA.; McKeown, NM (reprint author), Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. EM nicola.mckeown@tufts.edu FU International Life Science Institute North America Branch, Technical Committee on Carbohydrates FX This project was supported by a research grant from the International Life Science Institute North America Branch, Technical Committee on Carbohydrates [http://www.ilsi.org/Pages/HomePage.aspx]. Members of the ILSI NA Technical Committee on Carbohydrates, as experts in the field of fiber research, offered insights on fiber terms included in the Medline search and reviewed the manuscript. They had no role in the study design, data collection and analysis, results, decision to publish, or conclusions expressed in the manuscript. NR 25 TC 1 Z9 1 U1 1 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 27 PY 2016 VL 11 IS 6 AR e0156961 DI 10.1371/journal.pone.0156961 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DP9FD UT WOS:000378801200004 PM 27348733 ER PT J AU Al-Naqeb, G Rousova, J Kubatova, A Picklo, MJ AF Al-Naqeb, Ghanya Rousova, Jana Kubatova, Alena Picklo, Matthew J., Sr. TI Pulicaria jaubertii E-Gamal-Eldin reduces triacylglyceride content and modifies cellular antioxidant pathways in 3T3-L1 adipocytes SO CHEMICO-BIOLOGICAL INTERACTIONS LA English DT Article DE 3T3-L1; Pulicaria jaubertii; NQO1; Glutathione; Catechin; Carvotanacetone; Adipocyte ID NAD(P)H-QUINONE OXIDOREDUCTASE-1 NQO1; CHEMICAL-COMPOSITION; EPIGALLOCATECHIN GALLATE; INHIBITS ADIPOGENESIS; INSULIN SENSITIVITY; BIOLOGICAL-ACTIVITY; ADIPOSE-TISSUE; ESSENTIAL OIL; OBESITY; GLUTATHIONE AB Levels of obesity in Middle Eastern countries are increasing. Phytochemicals have anti-obesogenic properties as evidenced by prevention of adipocyte differentiation and blocking triacylglyceride (TG) accumulation. In Yemen, Pulicaria jaubertii E. Gamal-Eldin (PJ) is a food additive and a traditional medicine. We tested the hypothesis that phytochemicals present in PJ inhibit adipocytic responses during differentiation of 3T3-L1 preadipocytes to adipocytes. Methanolic extracts of PJ did not block expression of fatty acid binding protein 4 (FABP4) a marker of differentiation but did inhibit TG accumulation. Treatment of 3T3-L1 preadipocytes increased NADPH: quinone oxidoreductase 1 (NQO1), a suppressor of TG accumulation. Further fractionation of the methanolic PJ extract with hexane and dichloromethane (DCM) demonstrated that bioactivity towards TG reduction and elevated expression of NQO1 and other antioxidant genes (glutamate cysteine ligase catalytic unit, glutathione disulfide reductase, glutathione peroxidase (GPx) 4 resided in the DCM fraction. Activity towards depleting GSH and elevating the expression of catalase and GPx3 were found in the DCM and hexane fractions. Analysis by gas chromatography and liquid chromatography coupled with mass spectrometry demonstrated the presence of catechin-like moieties in the DCM and methanolic fractions and suggest that these components were partially responsible for the bioactivity of these fractions. In summary, our data indicate that fractions derived PJ exhibit anti-adipogenic properties in part through the presence of catechin-like compounds. Published by Elsevier Ireland Ltd. C1 [Al-Naqeb, Ghanya; Picklo, Matthew J., Sr.] USDA ARS, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave North, Grand Forks, ND 58203 USA. [Rousova, Jana; Kubatova, Alena; Picklo, Matthew J., Sr.] Univ N Dakota, Dept Chem, Grand Forks, ND 58202 USA. [Al-Naqeb, Ghanya] Sanaa Univ, Fac Agr, Dept Food Sci & Technol, Sanaa, Yemen. RP Picklo, MJ (reprint author), USDA ARS, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave North, Grand Forks, ND 58203 USA. EM matthew.picklo@ars.usda.gov FU USDA-ARS Projects [5450-51000-048-00D] FX The authors are grateful to Mary Briske-Anderson, Kim Michelson, Gregoire Brian and Dale Christopherson for their technical assistance with this work. Funding was provided through USDA-ARS Projects 5450-51000-048-00D. The U.S. Department of Agriculture, Agricultural Research Service, Plains Area is an equal opportunity/affirmative action employers and all agency services are available without discrimination. Mention of trade names or commercial products in this article is solely for providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 35 TC 0 Z9 0 U1 3 U2 7 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0009-2797 EI 1872-7786 J9 CHEM-BIOL INTERACT JI Chem.-Biol. Interact. PD JUN 25 PY 2016 VL 253 BP 48 EP 59 DI 10.1016/j.cbi.2016.05.013 PG 12 WC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Toxicology SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Toxicology GA DN9BA UT WOS:000377372800006 PM 27163856 ER PT J AU Wiersema, JH Gandhi, KN AF Wiersema, John H. Gandhi, Kanchi N. TI (20-22) Proposals to add Gerard, Fl. Gallo-Prov. 1761, Kramer, Elench. Veg. 1756, and Miller, Gard. Diet. Abr. ed. 4.1754 to the list of suppressed works in Appendix VI SO TAXON LA English DT Editorial Material C1 [Wiersema, John H.] USDA ARS, Natl Germplasm Resources Lab, Beltsville Agr Res Ctr BARC West, Bldg 003, Beltsville, MD 20705 USA. [Gandhi, Kanchi N.] Harvard Univ, Harvard Univ Herbaria, 22 Divin Ave, Cambridge, MA 02138 USA. RP Wiersema, JH (reprint author), USDA ARS, Natl Germplasm Resources Lab, Beltsville Agr Res Ctr BARC West, Bldg 003, Beltsville, MD 20705 USA. EM john.wiersema@ars.usda.gov NR 35 TC 0 Z9 0 U1 0 U2 0 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 EI 1996-8175 J9 TAXON JI Taxon PD JUN 24 PY 2016 VL 65 IS 3 BP 638 EP 639 DI 10.12705/653.23 PG 2 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA DQ5YJ UT WOS:000379280900023 ER PT J AU Wiersema, JH Greuter, W McNeill, J AF Wiersema, John H. Greuter, Werner McNeill, John TI (234-241) Some proposals to resolve problems relating to the conservation or rejection of names, suppression of works, and binding decisions SO TAXON LA English DT Editorial Material C1 [Wiersema, John H.] USDA ARS, Natl Germplasm Resources Lab, Beltsville Agr Res Ctr BARC West, Bldg 003, Beltsville, MD 20705 USA. [Greuter, Werner] Free Univ Berlin, Bot Garten, Konigin Luise Str 6-8, D-14195 Berlin, Germany. [Greuter, Werner] Free Univ Berlin, Bot Museum Berlin, Konigin Luise Str 6-8, D-14195 Berlin, Germany. [Greuter, Werner] Orto Bot, Herbarium Mediterraneum, Via Lincoln 2-A, I-90133 Palermo, Italy. [McNeill, John] Royal Bot Garden, 20A Inverleith Row, Edinburgh EH3 5LR, Midlothian, Scotland. RP Wiersema, JH (reprint author), USDA ARS, Natl Germplasm Resources Lab, Beltsville Agr Res Ctr BARC West, Bldg 003, Beltsville, MD 20705 USA. EM john.wiersema@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 EI 1996-8175 J9 TAXON JI Taxon PD JUN 24 PY 2016 VL 65 IS 3 BP 642 EP 646 DI 10.12705/653.26 PG 5 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA DQ5YJ UT WOS:000379280900026 ER PT J AU Wiersema, JH Greuter, W AF Wiersema, John H. Greuter, Werner TI (262) A proposal concerning the valid publication of suprageneric "autonyms" SO TAXON LA English DT Editorial Material C1 [Wiersema, John H.] USDA ARS, Natl Germplasm Resources Lab, Beltsville Agr Res Ctr BARC West, Bldg 003, Beltsville, MD 20705 USA. [Greuter, Werner] Free Univ Berlin, Bot Garten, Konigin Luise Str 6-8, D-14195 Berlin, Germany. [Greuter, Werner] Free Univ Berlin, Bot Museum Berlin, Konigin Luise Str 6-8, D-14195 Berlin, Germany. [Greuter, Werner] Orto Bot, Herbarium Mediterraneum, Via Lincoln 2-A, I-90133 Palermo, Italy. RP Wiersema, JH (reprint author), USDA ARS, Natl Germplasm Resources Lab, Beltsville Agr Res Ctr BARC West, Bldg 003, Beltsville, MD 20705 USA. EM john.wiersema@ars.usda.gov NR 3 TC 0 Z9 0 U1 0 U2 0 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 EI 1996-8175 J9 TAXON JI Taxon PD JUN 24 PY 2016 VL 65 IS 3 BP 652 EP 652 DI 10.12705/653.33 PG 1 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA DQ5YJ UT WOS:000379280900033 ER PT J AU Knapp, S Turland, NJ Barkworth, ME Barrie, FR Fortunato, RH Gandhi, K Gereau, RE Greuter, W Herendeen, PS Landrum, LR Mabberley, DJ Marhold, K May, TW Moore, G Arce, LR Smith, GF Thiele, K Zhang, L AF Knapp, Sandra Turland, Nicholas J. Barkworth, Mary E. Barrie, Fred R. Fortunato, Renee H. Gandhi, Kanchi Gereau, Roy E. Greuter, Werner Herendeen, Patrick S. Landrum, Leslie R. Mabberley, David J. Marhold, Karol May, Tom W. Moore, Gerry Arce, Lourdes Rico Smith, Gideon F. Thiele, Kevin Zhang, Li CA Special Comm By- TI (286) Proposal to replace Division III of the International Code of Nomenclature for algae, fungi, and plants SO TAXON LA English DT Editorial Material C1 [Knapp, Sandra] Nat Hist Museum, Dept Life Sci, Cromwell Rd, London SW7 5BD, England. [Turland, Nicholas J.] Free Univ Berlin, Bot Garten & Bot Museum Berlin, Konigin Luise Str 6-8, D-14195 Berlin, Germany. [Barkworth, Mary E.] Utah State Univ, Intermt Herbarium, Logan, UT 84322 USA. [Barrie, Fred R.] Missouri Bot Garden, POB 299, St Louis, MO 63166 USA. [Barrie, Fred R.] Field Museum, Dept Sci & Educ, 1400 S Lake Shore Dr, Chicago, IL 60605 USA. [Fortunato, Renee H.] INTA, RA-1686 Buenos Aires, DF, Argentina. [Fortunato, Renee H.] Consejo Nacl Invest Cient & Tecn, Inst Recursos Biol, Nicolas Repetto & Los Reseros S-N, RA-1686 Buenos Aires, DF, Argentina. [Gandhi, Kanchi] Harvard Univ Herbaria, 22 Divin Ave, Cambridge, MA 02138 USA. [Gereau, Roy E.] Missouri Bot Garden, POB 299, St Louis, MO 63166 USA. [Greuter, Werner] Free Univ Berlin, Bot Garten & Bot Museum Berlin, Konigin Luise Str 6-8, D-14195 Berlin, Germany. [Greuter, Werner] Orto Bot Palermo, Via Lincoln 2, Palermo, PA, Italy. [Herendeen, Patrick S.] Chicago Bot Garden, 1000 Lake Cook Rd, Glencoe, IL 60022 USA. [Landrum, Leslie R.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Mabberley, David J.] Univ Oxford, Wadham Coll, Oxford OX1 2JD, England. [Mabberley, David J.] Leiden Univ, Leiden, Netherlands. [Mabberley, David J.] Naturalis Biodivers Ctr, Leiden, Netherlands. [Mabberley, David J.] Macquarie Univ, Dept Biol Sci, Sydney, NSW, Australia. [Mabberley, David J.] Natl Herbarium New South Wales, Mrs Macquaries Rd, Sydney, NSW 2000, Australia. [Marhold, Karol] Slovak Acad Sci, Inst Bot, Bratislava 84523, Slovakia. [Marhold, Karol] Charles Univ Prague, Dept Bot, Fac Sci, Benatska 2, Prague 12801 2, Czech Republic. [May, Tom W.] Royal Bot Gardens Victoria, 100 Birdwood Ave, Melbourne, Vic 3004, Australia. [Moore, Gerry] USDA, Natl Plant Data Team, East Natl Technol Support Ctr, Nat Resources Conservat Serv, 2901 East Gate City Blvd, Greensboro, NC 27401 USA. [Arce, Lourdes Rico] Royal Bot Gardens, SC ID & Naming, Africa & Madagascar Team, Richmond TW9 3AE, Surrey, England. [Smith, Gideon F.] Nelson Mandela Metropolitan Univ, Dept Bot, POB 77000, ZA-6031 Port Elizabeth, South Africa. [Smith, Gideon F.] Univ Coimbra, Dept Ciencias Vida, Ctr Funct Ecol, P-3001455 Coimbra, Portugal. [Thiele, Kevin] Western Australia Herbarium, Dept Pk & Wildlife, Bentley Delivery Ctr, Locked Bag 104, Kensington, WA 6983, Australia. [Thiele, Kevin] Univ Western Australia, Sch Plant Biol, 35 Stirling Highway, Perth, WA 6009, Australia. [Zhang, Li] Shenzhen & Chinese Acad Sci, Fairy Lake Bot Garden, 160 Xianhu Rd, Shenzhen 518004, Guangdong, Peoples R China. RP Knapp, S (reprint author), Nat Hist Museum, Dept Life Sci, Cromwell Rd, London SW7 5BD, England. EM s.knapp@nhm.ac.uk RI Marhold, Karol/B-4699-2011; OI Marhold, Karol/0000-0002-7658-0844; May, Tom/0000-0003-2214-4972 NR 0 TC 2 Z9 2 U1 0 U2 2 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 EI 1996-8175 J9 TAXON JI Taxon PD JUN 24 PY 2016 VL 65 IS 3 BP 661 EP 664 DI 10.12705/653.41 PG 4 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA DQ5YJ UT WOS:000379280900041 ER PT J AU Knapp, S Turland, NJ Barkworth, ME Barrie, FR Fortunato, RH Gandhi, K Gereau, RE Greuter, W Herendeen, PS Landrum, LR Mabberley, DJ Marhold, K May, TW Moore, G Arce, LR Smith, GF Thiele, K Zhang, L AF Knapp, Sandra Turland, Nicholas J. Barkworth, Mary E. Barrie, Fred R. Fortunato, Renee H. Gandhi, Kanchi Gereau, Roy E. Greuter, Werner Herendeen, Patrick S. Landrum, Leslie R. Mabberley, David J. Marhold, Karol May, Tom W. Moore, Gerry Arce, Lourdes Rico Smith, Gideon F. Thiele, Kevin Zhang, Li TI Report of the Special Committee on By-laws for the Nomenclature Section SO TAXON LA English DT Article DE Code; Division III; International Botanical Congress; Nomenclature; Nomenclature Section ID ACACIA MILL.; CONTROVERSY; MELBOURNE AB The Special Committee on By-laws for the Nomenclature Section was established at the XVIII International Botanical Congress (IBC) in Melbourne in 2011, with the mandate to formalize the procedures by which changes to the Code are considered and voted upon by the Nomenclature Section, and to report to the XIX IBC in Shenzhen in 2017. With the wider participation of the community in nomenclature in general, and to address some past contentious issues in particular, the need for a clearer set of rules for management of the Nomenclature Section had surfaced. In light of this, the Committee conducted extensive negotiations and discussions on a wide range of governance topics, which are here summarized in the Committee's report. We propose the adoption of a new and greatly expanded version of Division III of the Code. This report provides the supporting documentation for the proposal that is also published in this issue (Knapp & al. in Taxon 65: 661-664. 2016). It is necessary that the two documents be read alongside each other. C1 [Knapp, Sandra] Nat Hist Museum, Dept Life Sci, Cromwell Rd, London SW7 5BD, England. [Turland, Nicholas J.] Free Univ Berlin, Bot Garten & Bot Museum Berlin, Konigin Luise Str 6-8, D-14195 Berlin, Germany. [Barkworth, Mary E.] Utah State Univ, Intermt Herbarium, Logan, UT 84322 USA. [Barrie, Fred R.] Missouri Bot Garden, POB 299, St Louis, MO 63166 USA. [Barrie, Fred R.] Field Museum, Dept Sci & Educ, 1400 S Lake Shore Dr, Chicago, IL 60605 USA. [Fortunato, Renee H.] INTA, RA-1686 Buenos Aires, DF, Argentina. [Fortunato, Renee H.] Consejo Nacl Invest Cient & Tecn, Inst Recursos Biol, Nicolcis Repetto & Reseros S-N, RA-1686 Buenos Aires, DF, Argentina. [Gandhi, Kanchi] Harvard Univ Herbaria, 22 Divin Ave, Cambridge, MA 02138 USA. [Gereau, Roy E.] Missouri Bot Garden, POB 299, St Louis, MO 63166 USA. [Greuter, Werner] Free Univ Berlin, Bot Garten & Bot Museum Berlin, Konigin Luise Str 6-8, D-14195 Berlin, Germany. [Greuter, Werner] Orto Bot Palermo, Via Lincoln 2, Palermo, PA, Italy. [Herendeen, Patrick S.] Chicago Bot Garden, 1000 Lake Cook Rd, Glencoe, IL 60022 USA. [Landrum, Leslie R.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Mabberley, David J.] Univ Oxford, Wadham Coll, Oxford OX1 2JD, England. [Mabberley, David J.] Leiden Univ, Leiden, Netherlands. [Mabberley, David J.] Naturalis Biodivers Ctr, Leiden, Netherlands. [Mabberley, David J.] Macquarie Univ, Dept Biol Sci, Sydney, NSW, Australia. [Mabberley, David J.] Natl Herbarium New South Wales, Mrs Macquaries Rd, Sydney, NSW 2000, Australia. [Marhold, Karol] Slovak Acad Sci, Inst Bot, Bratislava 84523, Slovakia. [Marhold, Karol] Charles Univ Prague, Dept Bot, Fac Sci, Benatska 2, Prague 12801 2, Czech Republic. [May, Tom W.] Royal Bot Gardens Victoria, 100 Birdwood Ave, Melbourne, Vic 3004, Australia. [Moore, Gerry] USDA, Natl Plant Data Team, East Natl Technol Support Ctr, Nat Resources Conservat Serv, 2901 East Gate City Blvd, Greensboro, NC 27401 USA. [Arce, Lourdes Rico] Royal Bot Gardens, SC ID & Naming, Africa & Madagascar Team, Richmond TW9 3AE, Surrey, England. [Smith, Gideon F.] Nelson Mandela Metropolitan Univ, Dept Bot, POB 77000, ZA-6031 Port Elizabeth, South Africa. [Smith, Gideon F.] Univ Coimbra, Dept Ciencias Vida, Ctr Funct Ecol, P-3001455 Coimbra, Portugal. [Thiele, Kevin] Western Australia Herbarium, Bentley Delivery Ctr, Dept Pk & Wildlife, Locked Bag 104, Kensington, WA, Australia. [Thiele, Kevin] Univ Western Australia, Sch Plant Biol, 35 Stirling Highway, Perth, WA 6009, Australia. [Zhang, Li] Fairy Lake Bot Garden, Shenzhen, Peoples R China. [Zhang, Li] Chinese Acad Sci, 160 Xianhu Rd, Shenzhen 518004, Guangdong, Peoples R China. RP Knapp, S (reprint author), Nat Hist Museum, Dept Life Sci, Cromwell Rd, London SW7 5BD, England. EM s.knapp@nhm.ac.uk RI Marhold, Karol/B-4699-2011; OI Marhold, Karol/0000-0002-7658-0844; May, Tom/0000-0003-2214-4972 NR 18 TC 1 Z9 1 U1 0 U2 1 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 EI 1996-8175 J9 TAXON JI Taxon PD JUN 24 PY 2016 VL 65 IS 3 BP 665 EP 669 DI 10.12705/653.42 PG 5 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA DQ5YJ UT WOS:000379280900042 ER PT J AU Fulton, RW d'Offay, JM Landis, C Miles, DG Smith, RA Saliki, JT Ridpath, JF Confer, AW Neill, JD Eberle, R Clement, TJ Chase, CCL Burge, LJ Payton, ME AF Fulton, R. W. d'Offay, J. M. Landis, C. Miles, D. G. Smith, R. A. Saliki, J. T. Ridpath, J. F. Confer, A. W. Neill, J. D. Eberle, R. Clement, T. J. Chase, C. C. L. Burge, L. J. Payton, M. E. TI Detection and characterization of viruses as field and vaccine strains in feedlot cattle with bovine respiratory disease SO VACCINE LA English DT Article DE Bovine respiratory viruses; MLV vaccines; Diagnostic virology ID VIRAL-DIARRHEA-VIRUS; PERSISTENTLY INFECTED CATTLE; TIME RT-PCR; GENETIC DIVERSITY; DIAGNOSTIC-TESTS; SYNCYTIAL VIRUS; UNITED-STATES; HERPESVIRUS-1; CORONAVIRUS; PATHOGENS AB This study investigated viruses in bovine respiratory disease (BRD) cases in feedlots, including bovine herpesvirus-1 (BoHV-1), bovine viral diarrhea virus (BVDV), bovine respiratory syncytial virus (BRSV), bovine coronaviruses (BoCV) and parainfluenza-3 virus (PI3V). Nasal swabs were collected from 114 cattle on initial BRD treatment. Processing included modified live virus (MLV) vaccination. Seven BRD necropsy cases were included for 121 total cases. Mean number of days on feed before first sample was 14.9 days. Swabs and tissue homogenates were tested by gel based PCR (G-PCR), quantitative-PCR (qPCR) and quantitative real time reverse transcriptase PCR (qRT-PCR) and viral culture. There were 87/114 (76.3%) swabs positive for at least one virus by at least one test. All necropsy cases were positive for at least one virus. Of 121 cases, positives included 18/121 (14.9%) BoHV-1; 19/121 (15.7%) BVDV; 76/121 (62.8%) BoCV; 11/121 (9.1%) BRSV; and 10/121 (8.3%) PI3V. For nasal swabs, G-PCR (5 viruses) detected 44/114 (38.6%); q-PCR and qRT-PCR (4 viruses) detected 81/114 (71.6%); and virus isolation detected 40/114 (35.1%). Most were positive for only one or two tests, but not all three tests. Necropsy cases had positives: 5/7 G-PCR, 5/7 q-PCR and qRT-PCR, and all were positive by cell culture. In some cases, G-PCR and both real time PCR were negative for BoHV-1, BVDV, and PI3V in samples positive by culture. PCR did not differentiate field from vaccines strains of BoHV-1, BVDV, and PI3V. However based on sequencing and analysis, field and vaccine strains of culture positive BoHV-1, BoCV, BVDV, and PI3V, 11/18 (61.1%) of BoHV-1 isolates, 6/17 (35.3%) BVDV isolates, and 1/10 (10.0%) PI3V identified as vaccine. BRSV was only identified by PCR testing. Interpretation of laboratory tests is appropriate as molecular based tests and virus isolation cannot separate field from vaccine strains. Additional testing using sequencing appears appropriate for identifying vaccine strains. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Fulton, R. W.; d'Offay, J. M.; Landis, C.; Confer, A. W.; Eberle, R.; Burge, L. J.] Oklahoma State Univ, Dept Vet Pathobiol, Stillwater, OK 74078 USA. [Miles, D. G.] Vet Res & Consulting Serv, Greeley, CO 80634 USA. [Smith, R. A.] Vet Res & Consulting Serv, Stillwater, OK 74075 USA. [Saliki, J. T.] Univ Georgia, Athens Vet Diagnost Lab, Athens, GA 30602 USA. [Ridpath, J. F.; Neill, J. D.] ARS, USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Clement, T. J.; Chase, C. C. L.] S Dakota State Univ, Dept Vet & Biomed Sci, Brookings, SD 57007 USA. [Payton, M. E.] Oklahoma State Univ, Dept Stat, Stillwater, OK 74078 USA. RP Fulton, RW (reprint author), Oklahoma State Univ, Dept Vet Pathobiol, Stillwater, OK 74078 USA. EM Robert.fulton@okstate.edu FU Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK from Endowed Chair, McCasland Foundation for Food Animal Research support; Wentz Scholars program; Niblack Research program FX This research was supported by the Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK via the research support from the Endowed Chair, McCasland Foundation for Food Animal Research support of Dr. R. Fulton. The Wentz Scholars program and the Niblack Research program supported the undergraduate research of Ms. Casey Landis. NR 37 TC 0 Z9 0 U1 10 U2 11 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X EI 1873-2518 J9 VACCINE JI Vaccine PD JUN 24 PY 2016 VL 34 IS 30 BP 3478 EP 3492 DI 10.1016/j.vaccine.2016.04.020 PG 15 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA DQ1IT UT WOS:000378955400008 PM 27108192 ER PT J AU Kim, HU Lee, KR Shim, D Lee, JH Chen, GQ Hwang, S AF Kim, Hyun Uk Lee, Kyeong-Ryeol Shim, Donghwan Lee, Jeong Hee Chen, Grace Q. Hwang, Seongbin TI Transcriptome analysis and identification of genes associated with omega-3 fatty acid biosynthesis in Perilla frutescens (L.) var. frutescens SO BMC GENOMICS LA English DT Article DE Perilla frutescens; Seed; omega-3 fatty acid; alpha-linolenic acid; Triacylglycerol; Transcriptome ID HYDROXY FATTY-ACID; CHLOROPLAST OMEGA-3 DESATURASE; PLUKENETIA-VOLUBILIS L.; TRANSGENIC ARABIDOPSIS; DIACYLGLYCEROL ACYLTRANSFERASE; ENZYMATIC-SYNTHESIS; DIFFERENTIAL EXPRESSION; MICROSOMAL PREPARATIONS; RICINOLEIC ACID; BRASSICA-RAPA AB Background: Perilla (Perilla frutescens (L.) var frutescens) produces high levels of a-linolenic acid (ALA), a omega-3 fatty acid important to health and development. To uncover key genes involved in fatty acid (FA) and triacylglycerol (TAG) synthesis in perilla, we conducted deep sequencing of cDNAs from developing seeds and leaves for understanding the mechanism underlying ALA and seed TAG biosynthesis. Results: Perilla cultivar Dayudeulkkae contains 66.0 and 56.2 % ALA in seeds and leaves, respectively. Using Illumina HiSeq 2000, we have generated a total of 392 megabases of raw sequences from four mRNA samples of seeds at different developmental stages and one mature leaf sample of Dayudeulkkae. De novo assembly of these sequences revealed 54,079 unique transcripts, of which 32,237 belong to previously annotated genes. Among the annotated genes, 66.5 % (21,429 out of 32,237) showed highest sequences homology with the genes from Mimulus guttatus, a species placed under the same Lamiales order as perilla. Using Arabidopsis acyl-lipid genes as queries, we searched the transcriptome and identified 540 unique perilla genes involved in all known pathways of acyl-lipid metabolism. We characterized the expression profiles of 43 genes involved in FA and TAG synthesis using quantitative PCR. Key genes were identified through sequence and gene expression analyses. Conclusions: This work is the first report on building transcriptomes from perilla seeds. The work also provides the first comprehensive expression profiles for genes involved in seed oil biosynthesis. Bioinformatic analysis indicated that our sequence collection represented a major transcriptomic resource for perilla that added valuable genetic information in order Lamiales. Our results provide critical information not only for studies of the mechanisms involved in ALA synthesis, but also for biotechnological production of ALA in other oilseeds. C1 [Kim, Hyun Uk; Hwang, Seongbin] Sejong Univ, Plant Engn Res Inst, Dept Bioind & Bioresource Engn, Seoul 05006, South Korea. [Lee, Kyeong-Ryeol] Rural Dev Adm, Natl Inst Agr Sci, Dept Agr Biotechnol, Jeonju 54874, South Korea. [Shim, Donghwan] Natl Inst Forest Sci, Dept Forest Genet Resources, Suwon 16631, South Korea. [Lee, Jeong Hee] SEEDERS Inc, Daejeon 34015, South Korea. [Chen, Grace Q.] USDA, ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. RP Kim, HU (reprint author), Sejong Univ, Plant Engn Res Inst, Dept Bioind & Bioresource Engn, Seoul 05006, South Korea. EM hukim64@sejong.ac.kr FU National Institute of Agricultural Science [PJ01007505]; Cooperative Research Program for Agricultural Science & Technology Development (SSAC) by Rural Development Administration (RDA), Republic of Korea [PJ01108101] FX This study was conducted with support from the National Institute of Agricultural Science (project no. PJ01007505) and the Cooperative Research Program for Agricultural Science & Technology Development (SSAC, Project No. PJ01108101) provided by the Rural Development Administration (RDA), Republic of Korea. USDA is an equal opportunity provider and employer. Mention of a specific product name by the United States Department of Agriculture does not constitute an endorsement and does not imply a recommendation over other suitable products. NR 73 TC 2 Z9 2 U1 9 U2 23 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUN 24 PY 2016 VL 17 AR 474 DI 10.1186/s12864-016-2805-0 PG 18 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DQ0MQ UT WOS:000378893800001 PM 27342315 ER PT J AU Huang, XQ Das, A Sahu, BB Srivastava, SK Leandro, LF O'Donnell, K Bhattacharyya, MK AF Huang, Xiaoqiu Das, Anindya Sahu, Binod B. Srivastava, Subodh K. Leandro, Leonor F. O'Donnell, Kerry Bhattacharyya, Madan K. TI Identification of Highly Variable Supernumerary Chromosome Segments in an Asexual Pathogen SO PLOS ONE LA English DT Article ID SUDDEN-DEATH SYNDROME; BEAN ROOT-ROT; COLLETOTRICHUM-GLOEOSPORIOIDES; FUSARIUM-VIRGULIFORME; TRANSPOSABLE ELEMENT; FILAMENTOUS FUNGI; SPECIES COMPLEX; SOUTH-AMERICA; EVOLUTION; CYTOCHROME-P450 AB Supernumerary chromosome segments are known to harbor different transposons from their essential counterparts. The aim of this study was to investigate the role of transposons in the origin and evolution of supernumerary segments in the asexual fungal pathogen Fusarium virguliforme. We compared the genomes of 11 isolates comprising six Fusarium species that cause soybean sudden death syndrome (SDS) or bean root rot (BRR), and identified significant levels of genetic variation in A+T-rich repeat blocks of the essential chromosomes and in A+T-neutral regions of the supernumerary segments. The A+T-rich repeat blocks in the essential chromosomes were highly variable between F. virguliforme and non-F. virguliforme isolates, but were scarcely variable between F. virguliforme isolates. The A+T-neutral regions in the supernumerary segments, however, were highly variable between F. virguliforme isolates, with a statistically significant number (21 standard deviations above the mean) of single nucleotide polymorphisms (SNPs). And supernumerary sequence types and rearrangement patterns of some F. virguliforme isolates were present in an isolate of F. cuneirostrum but not in the other F. virguliforme isolates. The most variable and highly expressed region in the supernumerary segments contained an active DNA transposon that was a most conserved match between F. virguliforme and the unrelated fungus Tolypocladiuminflatum. This transposon was absent from two of the F. virguliforme isolates. Furthermore, transposons in the supernumerary segments of some F. virguliforme isolates were present in non-F. virguliforme isolates, but were absent from the other F. virguliforme isolates. Two supernumerary P450 enzymes were 43% and 57% identical to their essential counterparts. This study has raised the possibility that transposons generate genetic variation in supernumerary chromosome segments by frequent horizontal transfer within and between closely related species. C1 [Huang, Xiaoqiu; Das, Anindya] Iowa State Univ, Dept Comp Sci, Ames, IA USA. [Huang, Xiaoqiu] Iowa State Univ, Inst Plant Sci, Ames, IA USA. [Sahu, Binod B.; Bhattacharyya, Madan K.] Iowa State Univ, Dept Agron, Ames, IA USA. [Srivastava, Subodh K.] Univ Arkansas, Crop Soil & Environm Sci, Fayetteville, AR 72701 USA. [Leandro, Leonor F.] Iowa State Univ, Dept Plant Pathol, Ames, IA USA. [O'Donnell, Kerry] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL USA. [Srivastava, Subodh K.] Clemson Univ, Dept Biochem & Genet, Clemson, SC USA. RP Huang, XQ (reprint author), Iowa State Univ, Dept Comp Sci, Ames, IA USA.; Huang, XQ (reprint author), Iowa State Univ, Inst Plant Sci, Ames, IA USA. EM xqhuang@iastate.edu OI Sahu, Binod Bihari/0000-0002-2151-0654 FU United States Department of Agriculture National Institute of Food and Agriculture; Iowa Soybean Association; Iowa State University; [2013-68004-20374] FX This project was supported by the United States Department of Agriculture National Institute of Food and Agriculture to MKB, LFL and XH (http://nifa.usda.gov and Grant no. 2013-68004-20374); Iowa Soybean Association to MKB (http://www.iasoybeans.com); and Iowa State University to XH (http://www.iastate.edu). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 50 TC 0 Z9 0 U1 2 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 24 PY 2016 VL 11 IS 6 AR e0158183 DI 10.1371/journal.pone.0158183 PG 23 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DP3KZ UT WOS:000378393600037 PM 27341103 ER PT J AU Bell, DM Schlaepfer, DR AF Bell, David M. Schlaepfer, Daniel R. TI On the dangers of model complexity without ecological justification in species distribution modeling SO ECOLOGICAL MODELLING LA English DT Article DE Prediction; Extrapolation; Model fitting; Species distribution modeling; Transferability ID CLIMATE-CHANGE IMPACTS; SPATIAL AUTOCORRELATION; VARIABLE SELECTION; RESPONSE CURVES; UNITED-STATES; RANGE; PREDICTION; SPACE; BIODIVERSITY; VALIDATION AB Although biogeographic patterns are the product of complex ecological processes, the increasing complexity of correlative species distribution models (SDMs) is not always motivated by ecological theory, but by model fit. The validity of model projections, such as shifts in a species' climatic niche, becomes questionable particularly during extrapolations, such as for future no-analog climate conditions. To examine the effects of model complexity on SDM predictive performance, we fit statistical models of varying complexity to simulated species occurrence data arising from data-generating processes that assume differing degrees of distributional symmetry in environmental space, interaction effects, and coverage in climate space. Mismatches between data-generating processes and statistical models (i.e., different functional forms) led to poor predictive performance when extrapolating to new climate-space and greater variation in extrapolated predictions for overly complex models. In contrast, performance issues were not apparent when using independent evaluation data from the training region. These results draw into question the use of highly flexible models for prediction without ecological justification. Published by Elsevier B.V. C1 [Bell, David M.] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Schlaepfer, Daniel R.] Univ Wyoming, Dept Bot, Laramie, WY 82071 USA. [Schlaepfer, Daniel R.] Univ Basel, Sect Conservat Biol, Dept Environm Sci, CH-4003 Basel, Switzerland. RP Bell, DM (reprint author), 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM dmbell@fs.fed.us RI Schlaepfer, Daniel/D-1756-2009 OI Schlaepfer, Daniel/0000-0001-9973-2065 FU NSF Postdoctoral Research Fellowship in Biology [DBI-1202800]; USDA Forest Service; University of Wyoming FX Funding for this work was provided by the NSF Postdoctoral Research Fellowship in Biology DBI-1202800 and the USDA Forest Service. We thank the University of Wyoming, Dr. W.K. Lauenroth, and Dr. J.B. Bradford for their support. This manuscript was improved by the comments of Dr. M.L. Mobley. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of NSF or the USDA Forest Service. NR 66 TC 1 Z9 1 U1 9 U2 29 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3800 EI 1872-7026 J9 ECOL MODEL JI Ecol. Model. PD JUN 24 PY 2016 VL 330 BP 50 EP 59 DI 10.1016/j.ecolmodel.2016.03.012 PG 10 WC Ecology SC Environmental Sciences & Ecology GA DL7MJ UT WOS:000375824500005 ER PT J AU Niu, ZQ Ascher, JS Luo, AR Griswold, T Zhu, CD AF Niu, Ze-Qing Ascher, John S. Luo, A-Rong Griswold, Terry Zhu, Chao-Dong TI Revision of the Anthidiellum Cockerell, 1904 of China (Hymenoptera, Apoidea, Megachilidae, Anthidiini) SO ZOOTAXA LA English DT Article DE Apiformes; taxonomy; combination; China; fauna AB The resin bees of the genus Anthidiellum Cockerell, 1904 are revised for China. Seven species are confirmed to occur in China including five new combinations: A. (Pycnanthidium) carinatum (Wu, 1962) comb. nov., A. (P.) coronum (Wu, 2004a) comb. nov., A. (P.) latipes (Bingham, 1897) comb. nov., A. (Clypanthidium) popovii (Wu, 1962) comb. nov., and A. (Anthidiellum) yunnanensis (Wu, 1962) comb. nov.. These five species had previously been classified as Trachusa (Paraanthidium), which is characterized by much larger-bodied bees (only four species of Trachusa (Paraanthidium) are confirmed to occur in China after this study; others reported in the literature were misplaced to genus). Additionally, Anthidiellum ludingensis Wu, 1993, and Anthidiellum (Anthidiellum) xinjiangensis Wu, 2004b, are removed from Anthidiellum forming the new combinations Pseudoanthidium (Pseudoanthidium) ludingense (Wu, 1993) and P. (P.) xinjiangense (Wu, 2004b), thus extending the range of the genus in China to include Sichuan. Illustrations and a key to known Chinese Anthidiellum species are provided. C1 [Niu, Ze-Qing; Luo, A-Rong; Zhu, Chao-Dong] Chinese Acad Sci, Inst Zool, Key Lab Zool Systemat & Evolut, 1 Beichen West Rd, Beijing 100101, Peoples R China. [Ascher, John S.] Natl Univ Singapore, Dept Biol Sci, 14 Sci Dr 4, Singapore 117543, Singapore. [Griswold, Terry] Utah State Univ, USDA ARS Bee Biol & Systemat Lab, Logan, UT 84322 USA. RP Zhu, CD (reprint author), Chinese Acad Sci, Inst Zool, Key Lab Zool Systemat & Evolut, 1 Beichen West Rd, Beijing 100101, Peoples R China. EM niuzq@ioz.ac.cn; dbsajs@nus.edu.sg; luoar@ioz.ac.cn; terry.griswold@ars.usda.gov; zhucd@ioz.ac.cn FU National Specific Research Funds for Public Benefit Department (Agriculture) [201303108]; NSFC Program [J1211002]; NSFC [31201701]; Key Laboratory of Zoological Systematics and Evolution, Chinese Academy of Sciences [Y229YX5105]; American Museum of Natural History FX This work was supported mainly by the National Specific Research Funds for Public Benefit Department (Agriculture) (Grant No. 201303108) to Ze-Qing NIU, the NSFC Program J1211002, the NSFC (Grant No. 31201701) to A-Rong LUO and the grant (No. Y229YX5105) from Key Laboratory of Zoological Systematics and Evolution, Chinese Academy of Sciences. John S. Ascher is grateful to Robert G. Goelet for his generous support of bee research at the American Museum of Natural History. Terry Grisworld was hosted by A-Rong LUO and her colleagues to study bee collection in the Institute of Zoology, Chinese Academy of Sciences. NR 27 TC 0 Z9 0 U1 1 U2 2 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD JUN 23 PY 2016 VL 4127 IS 2 BP 327 EP 344 DI 10.11646/zootaxa.4127.2.5 PG 18 WC Zoology SC Zoology GA DP5MA UT WOS:000378540400005 PM 27395626 ER PT J AU Pan, YD Schimel, D AF Pan, Yude Schimel, David TI BIOGEOCHEMISTRY Synergy of a warm spring and dry summer SO NATURE LA English DT Editorial Material AB An analysis suggests that high carbon uptake by US land ecosystems during the warm spring of 2012 offset the carbon loss that resulted from severe drought over the summer - and hints that the warm spring could have worsened the drought. C1 [Pan, Yude] US Forest Serv, Res Unit Climate Fire & Carbon Cycle Sci, USDA, Newtown Sq, PA 19073 USA. [Schimel, David] CALTECH, Jet Prop Lab, 4800 Oak Grove Dr, Pasadena, CA 91109 USA. RP Pan, YD (reprint author), US Forest Serv, Res Unit Climate Fire & Carbon Cycle Sci, USDA, Newtown Sq, PA 19073 USA. EM ypan@fs.fed.us NR 6 TC 0 Z9 0 U1 6 U2 13 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0028-0836 EI 1476-4687 J9 NATURE JI Nature PD JUN 23 PY 2016 VL 534 IS 7608 BP 483 EP 484 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DP1SS UT WOS:000378270300039 PM 27309806 ER PT J AU Glenn, DM AF Glenn, D. M. TI Effect of highly processed calcined kaolin residues on apple water use efficiency SO SCIENTIA HORTICULTURAE LA English DT Article DE Malus x domestica; Carbon isotopic discrimination; Photosynthesis; Transpiration; Fruit yield; Biomass; Evapotranspiration; Gas exchange; Radiation use efficiency ID PARTICLE FILM APPLICATION; FRUIT-QUALITY; GAS-EXCHANGE; EMPIRE APPLE; PHOTOSYNTHETIC RATE; SOLAR INJURY; LEAF; YIELD; LEAVES; SUNBURN AB Processed calcined kaolin particle films (PKPF) have been shown to repel some insects and reduce various environmental stresses often resulting in improved yield and quality of horticultural crops. PKPF studies in the literature have various water use efficiency (WUE) responses. The purpose of this study was to evaluate 2 application rates of PKPF (3% and 12%) on the whole tree water use efficiency and compare gas exchange values with carbon isotopic discrimination and seasonal water use efficiency in order to determine if there are consistent effects on WUE in apple and infer the mechanisms of action. Fruit yield and total biomass influence WUE whether WUE is measured directly in whole plant chambers, estimated by biomass/unit evapotranspiration, or Delta C-13. Delta C-13 analysis of WUE generally found that the untreated control had lower values and therefore higher WUE than PKPF treatments. Whole plant gas exchange analysis of WUE generally lacked the sensitivity to identify the treatment differences observed using Delta C-13 analysis and those treatment differences identified were contrary to Delta C-13 analysis. A lysimeter study supported the reduced WUE of PKPF treated plants by demonstrating increased E with a range of PKPF residue amounts. The lysimeter results suggest that for well-watered and presumably non-stressed conditions, gas exchange is limited by leaf temperature and the concomitant leaf-air vapor pressure deficit that influence stomatal conductance. When leaf temperature is reduced by PKPF, the response results in increased stomatal conductance and gas exchange, as measured by increased E. The reduction in WUE by PKPF treatment is balanced by an increase in overall gas exchange and increased yield and quality. Published by Elsevier B.V. C1 [Glenn, D. M.] ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. RP Glenn, DM (reprint author), ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. EM michael.glenn@ars.usda.gov FU Tessenderlo Kerley, Inc. FX The authors wish to thank Tessenderlo Kerley, Inc. for partial financial support of this research. NR 60 TC 0 Z9 0 U1 1 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD JUN 23 PY 2016 VL 205 BP 127 EP 132 DI 10.1016/j.scienta.2016.04.022 PG 6 WC Horticulture SC Agriculture GA DO5PP UT WOS:000377835500018 ER PT J AU Willett, CD Lerch, RN Lin, CH Goyne, KW Leigh, ND Roberts, CA AF Willett, C. D. Lerch, R. N. Lin, C. -H. Goyne, K. W. Leigh, N. D. Roberts, C. A. TI Benzoxazinone-Mediated Triazine Degradation: A Proposed Reaction Mechanism SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE benzoxazinones; atrazine; nonenzymatic atrazine degradation; DIBOA-Glc; hydroxyatrazine; phytochemistry ID TRIPSACUM-DACTYLOIDES; DEFENSE CHEMICALS; CORN SEEDLINGS; ATRAZINE; TRANSFORMATION; HYDROLYSIS; HERBICIDES; CATALYSIS; MISSOURI; SIMAZINE AB The role of benzoxazinones (Bx, 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one) in triazine resistance in plants has been studied for over half a century. In this research, fundamental parameters of the reaction between DIBOA-Glc (2-beta-D-glucopyranosyloxy-4-hydroxy-1,4-benzoxazin-3-one) and atrazine (ATR, 6-chloro-N-ethyl-N'-(1-methylethyl)-1,3,5-triazine-2,4-diamine) were examined. Through a series of experiments employing a variety of chromatographic and spectroscopic techniques, the DIBOA-Glc/ATR reaction was characterized in terms of reactant and product kinetics, stoichiometry, identification of a reaction intermediate, and reaction products formed. Results of these experiments demonstrated that the reaction mechanism proceeds via nucleophilic attack of the hydroxamic acid moiety of DIBOA-Glc at the C-2 position of the triazine ring to form hydroxyatrazine (HA, 2-hydroxy-4-ethylamino-6-isopropylamino-s-triazine), with associated degradation of DIBOA-Glc. Degradation of reactants followed first-order kinetics with a noncatalytic role of DIBOA-Glc. A reaction intermediate was identified as a DIBOA-Glc-HA conjugate, indicating a 1:1 DIBOA-Glc:ATR stoichiometry. Reaction products included HA and Cl-, but definitive identification of DIBOA-Glc reaction product(s) was not attained. With these reaction parameters elucidated, DIBOA-Glc can be evaluated in terms of its potential for a myriad of applications, including its use to address the problem of widespread ATR contamination of soil and water resources. C1 [Willett, C. D.; Goyne, K. W.] Univ Arkansas, Dept Crop Soil & Environm Sci, 1366 West Altheimer Dr, Fayetteville, AR 72704 USA. [Lerch, R. N.] USDA ARS, Cropping Syst & Water Qual Res Unit, 269 Ag Eng Bldg, Columbia, MO 65211 USA. [Lin, C. -H.] Univ Missouri, Dept Forestry, Ctr Agroforestry, 203 ABNR Bldg, Columbia, MO 65211 USA. [Leigh, N. D.] Missouri Univ Sci & Technol, Dept Chem, 142 Schrenk Hall,400 West 11th St, Rolla, MO 65409 USA. [Roberts, C. A.] Univ Missouri, Div Plant Sci, 214D Waters Hall, Columbia, MO 65211 USA. RP Willett, CD (reprint author), Univ Arkansas, Dept Crop Soil & Environm Sci, 1366 West Altheimer Dr, Fayetteville, AR 72704 USA. EM willettc@uark.edu NR 21 TC 0 Z9 0 U1 8 U2 12 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 22 PY 2016 VL 64 IS 24 BP 4858 EP 4865 DI 10.1021/acs.jafc.6b01017 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DP6CK UT WOS:000378584300005 PM 27215133 ER PT J AU Meepagala, KM Estep, AS Becnel, JJ AF Meepagala, Kumudini M. Estep, Alden S. Becnel, James J. TI Larvicidal and Adulticidal Activity of Chroman and Chromene Analogues against Susceptible and Permethrin-Resistant Mosquito Strains SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE mosquitoes; larvicide; repellents; chromenes; chromans; insecticide resistance ID BTI RESISTANCE; FEVER MOSQUITO; PESTICIDES; TOXICITY AB Mosquitoes play a major role as vectors that transmit parasitic and viral diseases worldwide, especially in tropical and subtropical countries. Mosquito borne diseases not only affect humans but they also affect livestock in many parts of the world. They carry diseases that are lethal to dogs and horses. Dog heartworm disease (Dirofilaria immitis) is a parasitic disease spread through mosquitoes. This disease is not limited to dogs, but it can affect other animals and humans as well. Eastern equine encephalitis (EEE) and West Nile virus (WNV) are also mosquito borne diseases that affect the central nervous system of horses and cause severe complications and death. Emergence of resistance among mosquitoes to current pesticides has increased the importance of the search for alternate compounds that are effective and environmentally benign with diverse modes of actions than those that are commercially available. Aedes aegypti mosquitoes are the primary vector for transmission of Zika viral fever, yellow fever, dengue fever, and chikungunya. Mosquito control is currently the best strategy to prevent mosquito borne diseases. There are numerous approaches for control of potentially dangerous mosquito populations. These approaches include the use of adulticides (insecticides), larvicides, and, to a limited extent, the use of repellents. Our previous studies have shown the mosquito repellent activity of chromenes. In the present study, we demonstrate larvicidal and adulticidal activity of chroman and chromene analogues against a permethrin susceptible laboratory strain as well as activity against a permethrin-resistant strain of Aedes aegypti. C1 [Meepagala, Kumudini M.] ARS, NPURU, USDA, POB 8048, University, MS 38677 USA. [Estep, Alden S.; Becnel, James J.] ARS, CMAVE, USDA, MFRU1600-1700 SW 23rd Dr, Gainesville, FL 32608 USA. [Estep, Alden S.] NASJAX, Navy Entomol Ctr Excellence NECE, 937 Child St, Jacksonville, FL 32212 USA. RP Meepagala, KM (reprint author), ARS, NPURU, USDA, POB 8048, University, MS 38677 USA. EM kmeepaga@olemiss.edu FU Deployed War Fighter Protection (DWFP) Research Program of the U.S. Department of Defense through the Armed Forces Pest Management Board FX This study was partly supported by the Deployed War Fighter Protection (DWFP) Research Program of the U.S. Department of Defense through the Armed Forces Pest Management Board. NR 20 TC 0 Z9 0 U1 7 U2 16 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 22 PY 2016 VL 64 IS 24 BP 4914 EP 4920 DI 10.1021/acs.jafc.6b01299 PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DP6CK UT WOS:000378584300012 PM 27249182 ER PT J AU Goyal, RK Fatima, T Topuz, M Bernadec, A Sicher, R Handa, AK Mattoo, AK AF Goyal, Ravinder K. Fatima, Tahira Topuz, Muhamet Bernadec, Anne Sicher, Richard Handa, Avtar K. Mattoo, Autar K. TI Pathogenesis-Related Protein 1b1 (PR1b1) Is a Major Tomato Fruit Protein Responsive to Chilling Temperature and Upregulated in High Polyamine Transgenic Genotypes SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE low temperature response; Solanum lycopersicum; cold responsive genes; methyl jasmonate; PR proteins; genetic engineering; 1-MCP; ethylene ID CONFERS ENHANCED DEHYDRATION; MYB TRANSCRIPTION FACTOR; TOBACCO-MOSAIC-VIRUS; LEGUME COVER CROP; GENE-EXPRESSION; FREEZING TOLERANCE; COLD-ACCLIMATION; ARGININE DECARBOXYLASE; PHYTOPHTHORA-INFESTANS; COMPATIBLE SOLUTES AB Plants execute an array of mechanisms in response to stress which include upregulation of defense related-proteins and changes in specific metabolites. Polyamines - putrescine (Put), spermidine (Spd), and spermine (Spm) - are metabolites commonly found associated with abiotic stresses such as chilling stress. We have generated two transgenic tomato lines (556HO and 579HO) that express yeast S-adenosylmethionine decarboxylase and specifically accumulate Spd and Spm in fruits in comparison to fruits from control (556AZ) plants (Mehta et al., 2002). Tomato fruits undergo chilling injury at temperatures below 13 degrees C. The high Spd and Spm tomato together with the control azygous line were utilized to address role(s) of polyamines in chilling-injury signaling. Exposure to chilling temperature (2 degrees C) led to several-fold increase in the Put content in all the lines. Upon re-warming of the fruits at 20 degrees C, the levels of Spd and Spm increased further in the fruit of the two transgenic lines, the higher levels remaining stable for 15 days after re-warming as compared to the fruit from the control line. Profiling their steady state proteins before and after re-warming highlighted a protein of similar to 14 kD. Using proteomics approach, protein sequencing and immunoblotting, the similar to 14 kD protein was identified as the pathogenesis related protein 1b1 (PR1b1). The PR1b1 protein accumulated transiently in the control fruit whose level was barely detectable at d 15 post-warming while in the fruit from both the 556HO and 579HO transgenic lines PR1b1 abundance increased and remained stable till d 15 post warming. PR1b1 gene transcripts were found low in the control fruit with a visible accumulation only on d 15 post warming; however, in both the transgenic lines it accumulated and increased soon after rewarming being several-fold higher on day 2 while in 556HO line this increase continued until d 6 than the control fruit. The chilling-induced increase in PR1b1 protein seems independent of ethylene and methyl jasmonate signaling but may be linked to salicylic acid. We propose that polyamine-mediated sustained accumulation of PR1b1 protein in post-warmed chilled tomato fruit is a pre-emptive cold stress response and possibly a defense response mechanism related to Cold Stress-Induced Disease Resistance (SIDR) phenomenon. C1 [Goyal, Ravinder K.; Fatima, Tahira; Topuz, Muhamet; Bernadec, Anne; Mattoo, Autar K.] ARS, Sustainable Agr Syst Lab, USDA, Beltsville, MD USA. [Sicher, Richard] USDA ARS, Crop Syst & Global Change Lab, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD USA. [Handa, Avtar K.] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA. [Goyal, Ravinder K.] Lethbridge Res & Dev Ctr, Lethbridge, AB, Canada. [Fatima, Tahira] Univ Western Ontario, London, ON, Canada. [Bernadec, Anne] INRA ENSAT, Toulouse, France. RP Mattoo, AK (reprint author), ARS, Sustainable Agr Syst Lab, USDA, Beltsville, MD USA. EM autar.mattoo@ars.usda.gov NR 103 TC 1 Z9 1 U1 13 U2 19 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JUN 22 PY 2016 VL 7 AR 907 DI 10.3389/fpls.2016.00907 PG 14 WC Plant Sciences SC Plant Sciences GA DP0YI UT WOS:000378216700002 ER PT J AU Jiang, XF Zhang, L Yang, HX Sappington, TW Cheng, YX Luo, LZ AF Jiang, Xingfu Zhang, Lei Yang, Haixia Sappington, Thomas W. Cheng, Yunxia Luo, Li Zhi TI Biocontrol of the oriental armyworm, Mythimna separata, by the tachinid fly Exorista civilis is synergized by Cry1Ab protoxin SO SCIENTIFIC REPORTS LA English DT Article ID BACILLUS-THURINGIENSIS; BT-COTTON; LEPIDOPTERA-NOCTUIDAE; NATURAL ENEMIES; POPULATION-DYNAMICS; DIAMONDBACK MOTH; NORTHERN CHINA; HYMENOPTERA; BRACONIDAE; WALKER AB Tritrophic interactions between Mythimna separata, its tachinid parasite Exorista civilis and the Cry1Ab were examined. Although 6th instar M. separata mortality increased with increasing Cry1Ab concentration, some tolerance was evident. Likewise, parasitization by E. civilis resulted in only 18% host mortality. However, combination of Cry1Ab and E. civilis parasitization resulted in a significant Cry1Ab dose-dependent increase in mortality over that of either alone, including a 50-56% synergistic increase in efficacy at the two concentrations tested. Pupal weight, adult emergence and lifetime fecundity of M. separata derived from larvae surviving both agents were negatively affected. The ability of E. civilis to parasitize and subsequently develop on the host was not adversely influenced by Cry1Ab. Instead, pupation rate increased significantly among host larvae fed 3.125 mu g/g Cry1Ab diet. Overall, our results demonstrate that use of Cry1Ab to control M. separata not only is compatible with the use of the tachinid parasitoid, but that the two methods can act synergistically to manage this destructive pest, provide support for the safety of transgenic Cry1Ab Bt plants in China. This example of two independent pest management strategies acting synergistically against a difficult pest offers a new perspective of broad significance in striving for agricultural sustainability. C1 [Jiang, Xingfu; Zhang, Lei; Yang, Haixia; Cheng, Yunxia; Luo, Li Zhi] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. [Yang, Haixia; Luo, Li Zhi] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China. [Sappington, Thomas W.] Iowa State Univ, Dept Entomol, Genet Lab, USDA ARS Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. RP Jiang, XF (reprint author), Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. EM xfjiang@ippcaas.cn FU National Department of Public Benefit Research Foundation [201403031]; International Science and Technology Cooperation Program of China [2014DFR31250]; National Natural Science Foundation of China [31373947, 31301656, 31000850, 31071677]; Beijing Natural Science Foundation [6142017] FX This work was funded by projects under the National Department of Public Benefit Research Foundation (201403031), International Science and Technology Cooperation Program of China (2014DFR31250), the National Natural Science Foundation of China (No. 31373947, 31301656, 31000850, 31071677) and Beijing Natural Science Foundation (6142017). NR 52 TC 0 Z9 0 U1 10 U2 14 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUN 21 PY 2016 VL 6 AR 26873 DI 10.1038/srep26873 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DP1IU UT WOS:000378244500001 PM 27324469 ER PT J AU Kwon, H Agha, MA Smith, RC Nachman, RJ Marion-Poll, F Pietrantonio, PV AF Kwon, Hyeogsun Agha, Moutaz Ali Smith, Ryan C. Nachman, Ronald J. Marion-Poll, Frederic Pietrantonio, Patricia V. TI Leucokinin mimetic elicits aversive behavior in mosquito Aedes aegypti (L.) and inhibits the sugar taste neuron SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE neuropeptide GPCR; sucrose taste; sensory neuron; chemical target validation; feeding deterrent ID YELLOW-FEVER MOSQUITO; MALPIGHIAN TUBULES; CULISETA-INORNATA; IN-VIVO; ANOPHELES-GAMBIAE; DROSOPHILA; RECEPTOR; SENSILLA; ANALOGS; IDENTIFICATION AB Insect kinins (leucokinins) are multifunctional peptides acting as neurohormones and neurotransmitters. In females of the mosquito vector Aedes aegypti (L.), aedeskinins are known to stimulate fluid secretion from the renal organs (Malpighian tubules) and hindgut contractions by activating a G protein-coupled kinin receptor designated "Aedae-KR." We used protease-resistant kinin analogs 1728, 1729, and 1460 to evaluate their effects on sucrose perception and feeding behavior. In no-choice feeding bioassays (capillary feeder and plate assays), the analog 1728, which contains alpha-amino isobutyric acid, inhibited females from feeding on sucrose. It further induced quick fly-away or walk-away behavior following contact with the tarsi and the mouthparts. Electrophysiological recordings from single long labellar sensilla of the proboscis demonstrated that mixing the analog 1728 at 1 mM with sucrose almost completely inhibited the detection of sucrose. Aedae-KR was immunolocalized in contact chemosensory neurons in prothoracic tarsi and in sensory neurons and accessory cells of long labellar sensilla in the distal labellum. Silencing Aedae-KR by RNAi significantly reduced gene expression and eliminated the feeding-aversion behavior resulting from contact with the analog 1728, thus directly implicating the Aedae-KR in the aversion response. To our knowledge, this is the first report that kinin analogs modulate sucrose perception in any insect. The aversion to feeding elicited by analog 1728 suggests that synthetic molecules targeting the mosquito Aedae-KR in the labellum and tarsi should be investigated for the potential to discover novel feeding deterrents of mosquito vectors. C1 [Kwon, Hyeogsun; Pietrantonio, Patricia V.] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. [Agha, Moutaz Ali; Marion-Poll, Frederic] Univ Paris Saclay, Univ Paris 11, Inst Rech Dev, UMR Evolut Genomes Comportement Ecol,CNRS, F-91198 Gif Sur Yvette, France. [Kwon, Hyeogsun; Smith, Ryan C.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. [Nachman, Ronald J.] USDA, Insect Control & Cotton Dis Res Unit, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. [Marion-Poll, Frederic] Univ Paris Saclay, AgroParisTech, F-75005 Paris, France. RP Pietrantonio, PV (reprint author), Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA.; Marion-Poll, F (reprint author), Univ Paris Saclay, Univ Paris 11, Inst Rech Dev, UMR Evolut Genomes Comportement Ecol,CNRS, F-91198 Gif Sur Yvette, France.; Marion-Poll, F (reprint author), Univ Paris Saclay, AgroParisTech, F-75005 Paris, France. EM frederic.marion-poll@agroparistech.fr; p-pietrantonio@tamu.edu OI Smith, Ryan/0000-0003-0245-2265 FU Texas A&M University Statistics Department; Texas AgriLife Research; National Institute of Food and Agriculture; US Department of Agriculture/Department of Defense Deployed War-Fighter Protection Initiative [6202-22000-029-00D]; French National Research Agency Program DESIRABLE [ANR-12-ALID-0001]; Agricultural Experiment Station at Iowa State University FX We thank Drs. S. Vitha (Texas A&M University Microscopy and Imaging Center); L. Zwiebel for the anti-ORCO 7 antibody used in initial tests; L. Lambrechts (Institut Pasteur, Paris) for the mosquitoes used for electrophysiology; and the Biology Information Technology staff at Iowa State University. Analyses were funded by an award from the Texas A&M University Statistics Department. Drawings were a gift of Ms. H. J. Oh. H.K. was supported by a Vector Biology Grant from Texas AgriLife Research (to P.V.P.). P.V.P. was supported by National Institute of Food and Agriculture-Hatch Act funding. R.J.N. received support from US Department of Agriculture/Department of Defense Deployed War-Fighter Protection Initiative 6202-22000-029-00D. M.A.A. and F.M.-P. were supported by the French National Research Agency Program DESIRABLE ANR-12-ALID-0001. R.C.S. received funds from the Agricultural Experiment Station at Iowa State University. NR 53 TC 1 Z9 1 U1 6 U2 18 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JUN 21 PY 2016 VL 113 IS 25 BP 6880 EP 6885 DI 10.1073/pnas.1520404113 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DP1TN UT WOS:000378272400037 PM 27274056 ER PT J AU Aguirre, MB Diaz-Soltero, H Claps, LE Bottero, AS Triapitsyn, S Hasson, E Logarzo, GA AF Aguirre, M. B. Diaz-Soltero, H. Claps, L. E. Saracho Bottero, A. Triapitsyn, S. Hasson, E. Logarzo, G. A. TI Studies on the Biology of Hypogeococcus pungens (sensu stricto) (Hemiptera: Pseudococcidae) in Argentina to Aid the Identification of the Mealybug Pest of Cactaceae in Puerto Rico SO JOURNAL OF INSECT SCIENCE LA English DT Article DE Biological control; species complex; host race; Alternanthera, cacti ID SCALE INSECTS; REPRODUCTIVE-BIOLOGY; SOUTH-AFRICA; HOMOPTERA; COCCOIDEA; COCCINEA; WEEDS AB Hypogeococcus pungens Granara de Willink, sensu stricto, is a serious pest of cacti in Puerto Rico threating many Caribbean islands. A classical biological control program for H. pungens was initiated for Puerto Rico in 2010 with a survey for natural enemies of H. pungens in its native range of Argentina. Biological differences were observed between populations of H. pungens sampled on Amaranthaceae and Cactaceae. Molecular studies suggested that H. pungens populations from different host plant families are likely a complex of species. Our objective was to study the biology of H. pungens sensu stricto on specimens collected in the same locality and host plant as the holotype [Tucuman Province, Argentina; Alternanthera pungens Kunth (Amaranthaceae)]. We were interested in the reproductive biology of females, longevity and survival of adults, the effect of temperature on the development, and nymph performance (survival and development) on five Cactaceae species. We found that H. pungens s.s. showed marked biological differences from the populations collected on Cactaceae and exported to Australia for the biological control of the cactus Harrisia spp. The main differences were the presence of deuterotoky parthenogenesis and the fact that H. pungens did not attack Cactaceae in the laboratory. Our results provide biological evidence that H. pungens is a species complex. We propose that the population introduced to Australia is neither Hypogeococcus festerianus Lizer y Trelles nor H. pungens, but an undescribed species with three circuli, and that the Hypogeococcus pest of cacti in Puerto Rico is not H. pungens. C1 [Aguirre, M. B.; Logarzo, G. A.] FuEDEI, Simon Bolivar 1559, Buenos Aires, DF, Argentina. [Diaz-Soltero, H.] USDA, 1400 Independence Ave,SW 1154 South Bldg, Washington, DC 20250 USA. [Claps, L. E.; Saracho Bottero, A.] Inst Super Entomol Dr Abraham Willink INSUE, Miguel Lillo 205, RA-4000 San Miguel De Tucuman, Argentina. [Triapitsyn, S.] Univ Calif Riverside, Dept Entomol, Entomol Res Museum, Riverside, CA 92521 USA. [Hasson, E.] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Ecol Genet & Evoluc, Ciudad Univ Pab 2,C1428 EHA Buenos Aires, RA-1053 Buenos Aires, DF, Argentina. RP Aguirre, MB (reprint author), FuEDEI, Simon Bolivar 1559, Buenos Aires, DF, Argentina. EM redbell_@hotmail.com; hilda.diaz-soltero@aphis.usda.gov; luciaclap@gmail.com; andrea_saracho1308@hotmail.com; estebanhasson@yahoo.com.ar; glogarzo@fuedei.org FU USDA Invasive Species Coordination Program FX We thank Mariel Guala for her excellent technical assistance, Dario Ruiz and Maria Moore for help in collection of Pseudococcidae. Special thanks to Fabian Font from FFyB, Herbario Museo de Farmacobotanica Juan Dominguez, University of Buenos Aires for identifying the Cactaceae and Amaranthaceae species used in this study. We also thank Ilya Gavrilov from the Zoological Institute of the Russian Academy of Sciences, St. Petersburg, Russia and Cristina Hernandez for advice in scale reproductive biology. Finally, we thank Arabella Bugliani, Laura Varone, Willie Cabrera Walsh, Alejandro Sosa, and anonymous reviewers for thoughtful comments on the manuscript. This study was supported by the USDA Invasive Species Coordination Program in FY 14. NR 40 TC 0 Z9 0 U1 6 U2 6 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1536-2442 EI 2250-2645 J9 J INSECT SCI JI J Insect Sci. PD JUN 20 PY 2016 VL 16 DI 10.1093/jisesa/iew043 PG 7 WC Entomology SC Entomology GA DQ0DW UT WOS:000378869600007 ER PT J AU Zhu, YC Yao, JX Luttrell, R AF Zhu, Yu-Cheng Yao, Jianxiu Luttrell, Randall TI Identification of Genes Potentially Responsible for extra-Oral Digestion and Overcoming Plant Defense from Salivary Glands of the Tarnished Plant Bug (Hemiptera: Miridae) Using cDNA Sequencing SO JOURNAL OF INSECT SCIENCE LA English DT Article DE Lygus lineolaris; TPB; saliva; salivary gland; cDNA; polygalacturonases; extra-oral digestion; detoxification ID LYGUS-LINEOLARIS HETEROPTERA; PROTEASE INHIBITORS; MOLECULAR-CLONING; MYZUS-PERSICAE; RUSSIAN WHEAT; BT COTTON; APHID; PROTEINS; BIOLOGY; INSECTS AB Saliva is known to play a crucial role in tarnished plant bug (TPB, Lygus lineolaris [Palisot de Beauvois]) feeding. By facilitating the piercing, the enzyme-rich saliva may be used for extra-oral digestion and for overcoming plant defense before the plant fluids are ingested by TPBs. To identify salivary gland genes, mRNA was extracted from salivary glands and cDNA library clones were sequenced. A de novo-assembling of 7,000 Sanger sequences revealed 666 high-quality unique cDNAs with an average size of 624 bp, in which the identities of 347 cDNAs were determined using Blast2GO. Kyoto Encyclopedia of Genes and Genomes analysis indicated that these genes participate in eighteen metabolic pathways. Identifications of large number of enzyme genes in TPB salivary glands evidenced functions for extra-oral digestion and feeding damage mechanism, including 45 polygalacturonase, two a-amylase, one glucosidase, one glycan enzyme, one aminopeptidase, four lipase, and many serine protease cDNAs. The presence of multiple transcripts, multigene members, and high abundance of cell wall degradation enzymes (polygalacturonases) indicated that the enzyme-rich saliva may cause damage to plants by breaking down plant cell walls to make nutrients available for feeding. We also identified genes potentially involved in insect adaptation and detoxifying xenobiotics that may allow insects to overcome plant defense responses, including four glutathione S-transferases, three esterases, one cytochrome P450, and several serine proteases. The gene profiles of TPB salivary glands revealed in this study provides a foundation for further understanding and potential development of novel enzymatic inhibitors, or other RNAi approaches that may interrupt or minimize TPB feeding damage. C1 [Zhu, Yu-Cheng; Yao, Jianxiu; Luttrell, Randall] USDA ARS, Southern Insect Management Res Unit, Stoneville, MS 38776 USA. RP Zhu, YC (reprint author), USDA ARS, Southern Insect Management Res Unit, Stoneville, MS 38776 USA. EM yc.zhu@ars.usda.gov NR 61 TC 1 Z9 1 U1 5 U2 14 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1536-2442 EI 2250-2645 J9 J INSECT SCI JI J Insect Sci. PD JUN 20 PY 2016 VL 16 DI 10.1093/jisesa/iew041 PG 11 WC Entomology SC Entomology GA DQ0DW UT WOS:000378869600009 ER PT J AU Hess, AS Islam, Z Hess, MK Rowland, RRR Lunney, JK Doeschl-Wilson, A Plastow, GS Dekkers, JCM AF Hess, Andrew S. Islam, Zeenath Hess, Melanie K. Rowland, Raymond R. R. Lunney, Joan K. Doeschl-Wilson, Andrea Plastow, Graham S. Dekkers, Jack C. M. TI Comparison of host genetic factors influencing pig response to infection with two North American isolates of porcine reproductive and respiratory syndrome virus SO GENETICS SELECTION EVOLUTION LA English DT Article ID LACTATION CURVE; NEUTRALIZING ANTIBODIES; IMMUNE-RESPONSE; BREEDING VALUES; SYNDROME PRRS; RESISTANCE; SWINE; VIRULENCE; CATTLE; PATHOGENESIS AB Background: Porcine reproductive and respiratory syndrome (PRRS) is one of the most important swine diseases in the world and genetic selection of pigs for increased resistance to PRRS is an attractive method to improve the health status of the swine herd. This study compared phenotypic and genetic responses to infection with one of two genetically distinct type 2 PRRS virus (PRRSV) isolates: NVSL-97-7895 (NVSL) and KS-2006-72109 (KS06), and evaluated whether the single nucleotide polymorphism (SNP) WUR10000125 (WUR) on chromosome 4 that was associated with viral load and weight gain under infection with NVSL also has an effect on response to infection across North American PRRSV isolates. Wood's lactation curve was fitted to repeated viremia measurements to derive five curve characteristics that were evaluated. Results: Infection with NVSL was characterized by reaching a 14 +/- 2 % higher peak viremia (PV) 2.5 +/- 0.6 days earlier (time to peak; TP) than KS06, followed by 36 +/- 1 % faster virus clearance, which occurred 3.9 +/- 0.7 days sooner. Weight gain from 0 to 42 days post-infection (WG) tended to be higher under infection with KS06 than NVSL (3.7 +/- 1.5 kg). Estimates of heritability were moderate for both PRRSV isolates for viral load from 0 to 21 days post-infection (VL) (NVSL: 0.31 +/- 0.06; KS06: 0.51 +/- 0.09) and WG (NVSL: 0.33 +/- 0.06; KS06: 0.31 +/- 0.09). Strong negative genetic correlations were observed between VL and WG for both NVSL (-0.74 +/- 0.10) and KS06 (-0.52 +/- 0.17) infected pigs. Pigs with genotype AB at the WUR SNP had a more desirable phenotype than AA pigs for all traits under infection with NVSL, but only for VL and PV with KS06; effects on other traits were smaller and not significantly different from zero (P > 0.05). Genetic correlations of host response between isolates were strong for VL, WG and PV. Accounting for WUR genotype had little impact on these correlations, suggesting that response to PRRSV infection has a substantial polygenic component that is common between these two isolates. Conclusions: These results suggest that the KS06 PRRSV isolate is less virulent than NVSL but that genetic selection for increased resistance to either of these genetically distinct isolates is expected to increase resistance to the other isolate. C1 [Hess, Andrew S.; Hess, Melanie K.; Dekkers, Jack C. M.] Iowa State Univ, Dept Anim Sci, Ames, IA USA. [Islam, Zeenath; Doeschl-Wilson, Andrea] Univ Edinburgh, Roslin Inst & R D SVS, Edinburgh, Midlothian, Scotland. [Rowland, Raymond R. R.] Kansas State Univ, Coll Vet Med, Manhattan, KS 66506 USA. [Lunney, Joan K.] USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Plastow, Graham S.] Univ Alberta, Edmonton, AB, Canada. RP Dekkers, JCM (reprint author), Iowa State Univ, Dept Anim Sci, Ames, IA USA. EM jdekkers@iastate.edu FU Genome Canada; USDA-NIFA Grant [2013-68004-20362]; National Pork Board [12-061, 14-223] FX This project was funded by Genome Canada, USDA-NIFA Grant 2013-68004-20362 and National Pork Board Grants #12-061 and #14-223. The authors would also like to acknowledge contributions from members of the PRRS Host Genetics Consortium consisting of USDA ARS, Kansas State University, Iowa State University, Michigan State University, Washington State University, Purdue University, University of Nebraska-Lincoln, PIC/Genus, Newsham Choice Genetics, Fast Genetics, Genetiporc, Inc., Genesus, Inc., PigGen Canada, Inc., IDEXX Laboratories, and Tetracore, Inc. Contributions of the late Dr. Steve Bishop of the Roslin Institute are also acknowledged. Steve was involved in the design of the experiments and oversaw the fitting of the Wood's curves to the viremia data and estimation of the Wood's curve parameters. NR 59 TC 2 Z9 2 U1 3 U2 7 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0999-193X EI 1297-9686 J9 GENET SEL EVOL JI Genet. Sel. Evol. PD JUN 20 PY 2016 VL 48 AR 43 DI 10.1186/s12711-016-0222-0 PG 20 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA DP4SQ UT WOS:000378486900001 PM 27324857 ER PT J AU Niu, XW Zhao, XQ Ling, KS Levi, A Sun, YY Fan, M AF Niu, Xiaowei Zhao, Xiaoqiang Ling, Kai-Shu Levi, Amnon Sun, Yuyan Fan, Min TI The FonSIX6 gene acts as an avirulence effector in the Fusarium oxysporum f. sp niveum - watermelon pathosystem SO SCIENTIFIC REPORTS LA English DT Article ID AGROBACTERIUM-MEDIATED TRANSFORMATION; I-3-MEDIATED RESISTANCE; TOMATO; RACES; WILT; VIRULENCE AB When infecting a host plant, the fungus Fusarium oxysporum secretes several effector proteins into the xylem tissue to promote virulence. However, in a host plant with an innate immune system involving analogous resistance proteins, the fungus effector proteins may trigger resistance, rather than promoting virulence. Identity of the effector genes of Fusarium oxysporum f. sp. niveum (Fon) races that affect watermelon (Citrullus lanatus) are currently unknown. In this study, the SIX6 (secreted in xylem protein 6) gene was identified in Fon races 0 and 1 but not in the more virulent Fon race 2. Disrupting the FonSIX6 gene in Fon race 1 did not affect the sporulation or growth rate of the fungus but significantly enhanced Fon virulence in watermelon, suggesting that the mutant. Fon1SIX6 protein allowed evasion of R protein-mediated host resistance. Complementation of the wild-type race 2 (which lacks FonSIX6) with FonSIX6 reduced its virulence. These results provide evidence supporting the hypothesis that FonSIX6 is an avirulence gene. The identification of FonSix6 as an avirulence factor may be a first step in understanding the mechanisms of Fon virulence and resistance in watermelon and further elucidating the role of Six6 in Fusarium-plant interactions. C1 [Niu, Xiaowei; Zhao, Xiaoqiang; Sun, Yuyan; Fan, Min] Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou 310021, Zhejiang, Peoples R China. [Ling, Kai-Shu; Levi, Amnon] USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. RP Fan, M (reprint author), Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou 310021, Zhejiang, Peoples R China. EM fanminfm@sina.com FU National Natural Science Foundation of China [31301787, 31272188]; Natural Science Foundation of Zhejiang Province [LQ13C150004]; Major Science and Technology Project of Zhejiang Province [2012C12903] FX This research was supported by the National Natural Science Foundation of China (31301787) and (31272188), the Natural Science Foundation of Zhejiang Province (LQ13C150004), and the Major Science and Technology Project of Zhejiang Province (2012C12903). NR 26 TC 0 Z9 0 U1 11 U2 16 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUN 20 PY 2016 VL 6 AR 28146 DI 10.1038/srep28146 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DO8KB UT WOS:000378031100001 PM 27320044 ER PT J AU Ridpath, JF Neill, JD AF Ridpath, Julia F. Neill, John D. TI Challenges in Identifying and Determining the Impacts of Infection with Pestiviruses on the Herd Health of Free Ranging Cervid Populations SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE pestivirus; cervids; wildlife diseases; surveillance; sampling ID VIRAL-DIARRHEA-VIRUS; WHITE-TAILED DEER; MOUSEDEER TRAGULUS-JAVANICUS; SCOTTISH RED DEER; ODOCOILEUS-VIRGINIANUS; DISEASE SURVEILLANCE; PERSISTENT INFECTION; HEMORRHAGIC-DISEASE; MULE DEER; CATTLE AB Although most commonly associated with the infection of domestic livestock, the replication of pestiviruses, in particular the two species of bovine viral diarrhea virus (BVDV), occurs in a wide range of free ranging cervids including white-tailed deer, mule deer, fallow deer, elk, red deer, roe deer, eland and mousedeer. While virus isolation and serologic analyses indicate that pestiviruses are circulating in these populations, little is known regarding their impact. The lack of regular surveillance programs, challenges in sampling wild populations, and scarcity of tests and vaccines compound the difficulties in detecting and controlling pestivirus infections in wild cervids. Improved detection rests upon the development and validation of tests specific for use with cervid samples and development and validation of tests that reliably detect emerging pestiviruses. Estimation of impact of pestivirus infections on herd health will require the integration of several disciplines including epidemiology, cervid natural history, veterinary medicine, pathology and microbiology. C1 [Ridpath, Julia F.; Neill, John D.] ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. RP Ridpath, JF (reprint author), ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. EM julia.ridpath@ars.usda.gov NR 58 TC 0 Z9 0 U1 11 U2 11 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD JUN 17 PY 2016 VL 7 AR 921 DI 10.3389/fmicb.2016.00921 PG 7 WC Microbiology SC Microbiology GA DO7KC UT WOS:000377960300001 PM 27379051 ER PT J AU Thiel, V Wood, JM Olsen, MT Tank, M Klatt, CG Ward, DM Bryant, DA AF Thiel, Vera Wood, Jason M. Olsen, Millie T. Tank, Marcus Klatt, Christian G. Ward, David M. Bryant, Donald A. TI The Dark Side of the Mushroom Spring Microbial Mat: Life in the Shadow of Chlorophototrophs. I. Microbial Diversity Based on 16S rRNA Gene Amplicons and Metagenomic Sequencing SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE hot spring; microbial community; microbial diversity; extreme environments; phototrophic bacteria ID YELLOWSTONE-NATIONAL-PARK; CANDIDATUS CHLORACIDOBACTERIUM THERMOPHILUM; GRADIENT GEL-ELECTROPHORESIS; OPERATIONAL TAXONOMIC UNITS; ALGAL-BACTERIAL MATS; ALKALINE HOT-SPRINGS; CYANOBACTERIAL MAT; SP-NOV.; SYNECHOCOCCUS POPULATIONS; MOLECULAR SIGNATURES AB Microbial-mat communities in the effluent channels of Octopus and Mushroom Springs within the Lower Geyser Basin at Yellowstone National Park have been studied for nearly 50 years. The emphasis has mostly focused on the chlorophototrophic bacterial organisms of the phyla Cyanobacteria and Chloroflexi. In contrast, the diversity and metabolic functions of the heterotrophic community in the microoxic/anoxic region of the mat are not well understood. In this study we analyzed the orange-colored undermat of the microbial community of Mushroom Spring using metagenomic and rRNA-amplicon (iTag) analyses. Our analyses disclosed a highly diverse community exhibiting a high degree of unevenness, strongly dominated by a single taxon, the filamentous anoxygenic phototroph, Roseiflexus spp. The second most abundant organisms belonged to the Thermotogae, which have been hypothesized to be a major source of H-2 from fermentation that could enable photomixotrophic metabolism by Chloroflexus and Roseiflexus spp. Other abundant organisms include two members of the Armatimonadetes (OP10); Thermocrinis sp.; and phototrophic and heterotrophic members of the Chloroflexi. Further, an Atribacteria (OP9/JS1) member; a sulfate-reducing Therrnodesulfovibrio sp.; a Planctomycetes member; a member of the EM3 group tentatively affiliated with the Thermotogae, as well as a putative member of the Arrninicenantes (OP8) represented >= 1% of the reads. Archaea were not abundant in the iTag analysis, and no metagenomic bin representing an archaeon was identified. A high microdiversity of 16S rRNA gene sequences was identified for the dominant taxon, Roseiflexus spp. Previous studies demonstrated that highly similar Synechococcus variants in the upper layer of the mats represent ecological species populations with specific ecological adaptations. This study suggests that similar putative ecotypes specifically adapted to different niches occur within the undermat community, particularly for Roseiflexus spp. C1 [Thiel, Vera; Tank, Marcus; Bryant, Donald A.] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA. [Wood, Jason M.; Olsen, Millie T.; Klatt, Christian G.; Ward, David M.] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. [Klatt, Christian G.] Univ Minnesota, ARS, USDA, St Paul, MN 55108 USA. [Bryant, Donald A.] Montana State Univ, Dept Chem & Biochem, Bozeman, MT 59717 USA. [Thiel, Vera; Bryant, Donald A.] Tokyo Metropolitan Univ, Dept Biol Sci, Hachioji, Tokyo, Japan. RP Thiel, V; Bryant, DA (reprint author), Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA.; Bryant, DA (reprint author), Montana State Univ, Dept Chem & Biochem, Bozeman, MT 59717 USA.; Thiel, V; Bryant, DA (reprint author), Tokyo Metropolitan Univ, Dept Biol Sci, Hachioji, Tokyo, Japan. EM vthiel@tmu.ac.jp; dab14@psu.edu FU Division of Chemical Sciences, Geosciences, and Biosciences, Office of Basic Energy Sciences of the Department of Energy [DE-FG02-94ER20137]; NASA [NX09AM87G]; U.S. Department of Energy (DOE), Office of Biological and Environmental Research (BER), BER's Genomic Science Program 395 (GSP); Community Sequencing Program [CSP-411]; Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231] FX This study was partly funded by the Division of Chemical Sciences, Geosciences, and Biosciences, Office of Basic Energy Sciences of the Department of Energy through Grant DE-FG02-94ER20137. DB and DW additionally acknowledge support from the NASA Exobiology program (NX09AM87G). This work was also partly supported by the U.S. Department of Energy (DOE), Office of Biological and Environmental Research (BER), as part of BER's Genomic Science Program 395 (GSP). This contribution originates from the GSP Foundational Scientific Focus Area (FSFA) at the Pacific Northwest National Laboratory (PNNL) under a subcontract to DB. The nucleotide sequencing was performed as part of a Community Sequencing Program (Project CSP-411) and was performed by the U.S. Department of Energy Joint Genome Institute, which is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. NR 118 TC 0 Z9 0 U1 8 U2 16 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD JUN 17 PY 2016 VL 7 AR 919 DI 10.3389/fmicb.2016.00919 PG 25 WC Microbiology SC Microbiology GA DO7KB UT WOS:000377960200001 PM 27379049 ER PT J AU Raaijmakers, JM Mazzola, M AF Raaijmakers, Jos M. Mazzola, Mark TI Soil immune responses Soil microbiomes may be harnessed for plant health SO SCIENCE LA English DT Editorial Material ID RHIZOSPHERE MICROBIOME; FUSARIUM-WILT; SUPPRESSION; POPULATIONS C1 [Raaijmakers, Jos M.] Royal Netherlands Acad Arts & Sci, Netherlands Inst Ecol, Wageningen, Netherlands. [Raaijmakers, Jos M.] Leiden Univ, Inst Biol, Leiden, Netherlands. [Mazzola, Mark] USDA ARS, Wenatchee, WA USA. RP Raaijmakers, JM (reprint author), Royal Netherlands Acad Arts & Sci, Netherlands Inst Ecol, Wageningen, Netherlands.; Raaijmakers, JM (reprint author), Leiden Univ, Inst Biol, Leiden, Netherlands. EM j.raaijmakers@nioo.knaw.nl RI Raaijmakers, Jos/D-1574-2014 OI Raaijmakers, Jos/0000-0003-1608-6614 NR 14 TC 2 Z9 2 U1 28 U2 57 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 EI 1095-9203 J9 SCIENCE JI Science PD JUN 17 PY 2016 VL 352 IS 6292 BP 1392 EP 1393 DI 10.1126/science.aaf3252 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DO7PV UT WOS:000377975400021 PM 27313024 ER PT J AU Chai, BL Tsoi, TV Iwai, S Liu, C Fish, JA Gu, C Johnson, TA Zylstra, G Teppen, BJ Li, H Hashsham, SA Boyd, SA Cole, JR Tiedje, JM AF Chai, Benli Tsoi, Tamara V. Iwai, Shoko Liu, Cun Fish, Jordan A. Gu, Cheng Johnson, Timothy A. Zylstra, Gerben Teppen, Brian J. Li, Hui Hashsham, Syed A. Boyd, Stephen A. Cole, James R. Tiedje, James M. TI Sphingomonas wittichii Strain RW1 Genome-Wide Gene Expression Shifts in Response to Dioxins and Clay SO PLOS ONE LA English DT Article ID DIBENZO-P-DIOXIN; DEPENDENT TRANSPORT; DIOXYGENASE; SYSTEM; BIODEGRADATION; DEGRADATION; METABOLISM; MECHANISMS; MEMBRANE; SEQUENCE AB Sphingomonas wittichii strain RW1 (RW1) is one of the few strains that can grow on dibenzo-p-dioxin (DD). We conducted a transcriptomic study of RW1 using RNA-Seq to outline transcriptional responses to DD, dibenzofuran (DF), and the smectite clay mineral saponite with succinate as carbon source. The ability to grow on DD is rare compared to growth on the chemically similar DF even though the same initial dioxygenase may be involved in oxidation of both substrates. Therefore, we hypothesized the reason for this lies beyond catabolic pathways and may concern genes involved in processes for cell-substrate interactions such as substrate recognition, transport, and detoxification. Compared to succinate (SUC) as control carbon source, DF caused over 240 protein-coding genes to be differentially expressed, whereas more than 300 were differentially expressed with DD. Stress response genes were up-regulated in response to both DD and DF. This effect was stronger with DD than DF, suggesting a higher toxicity of DD compared to DF. Both DD and DF caused changes in expression of genes involved in active cross-membrane transport such as TonB-dependent receptor proteins, but the patterns of change differed between the two substrates. Multiple transcription factor genes also displayed expression patterns distinct to DD and DF growth. DD and DF induced the catechol ortho-and the salicylate/gentisate pathways, respectively. Both DD and DF induced the shared down-stream aliphatic intermediate compound pathway. Clay caused category-wide down-regulation of genes for cell motility and chemotaxis, particularly those involved in the synthesis, assembly and functioning of flagella. This is an environmentally important finding because clay is a major component of soil microbes' microenvironment influencing local chemistry and may serve as a geosorbent for toxic pollutants. Similar to clay, DD and DF also affected expression of genes involved in motility and chemotaxis. C1 [Chai, Benli; Tsoi, Tamara V.; Iwai, Shoko; Fish, Jordan A.; Johnson, Timothy A.; Cole, James R.; Tiedje, James M.] Michigan State Univ, Ctr Microbial Ecol, E Lansing, MI 48824 USA. [Liu, Cun; Johnson, Timothy A.; Teppen, Brian J.; Li, Hui; Boyd, Stephen A.] Michigan State Univ, Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Hashsham, Syed A.] Michigan State Univ, Civil & Environm Engn, E Lansing, MI 48824 USA. [Gu, Cheng] Nanjing Univ, Sch Environm, Nanjing 210008, Jiangsu, Peoples R China. [Zylstra, Gerben] Rutgers State Univ, Dept Biochem & Microbiol, New Brunswick, NJ 08903 USA. [Iwai, Shoko] Second Genome Inc, San Francisco, CA 94080 USA. [Johnson, Timothy A.] USDA ARS, Natl Anim Dis Ctr, Food Safety & Enter Pathogens Res Unit, POB 70, Ames, IA 50010 USA. RP Tiedje, JM (reprint author), Michigan State Univ, Ctr Microbial Ecol, E Lansing, MI 48824 USA. EM tiedjej@msu.edu RI Li, Hui/G-4055-2010 OI Li, Hui/0000-0003-3298-5265 FU National Institute of Environmental Health Sciences grant [5P42 ES004911-18/19] FX This study was supported by the National Institute of Environmental Health Sciences grant 5P42 ES004911-18/19. NR 46 TC 0 Z9 0 U1 6 U2 9 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 16 PY 2016 VL 11 IS 6 AR e0157008 DI 10.1371/journal.pone.0157008 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DO8JO UT WOS:000378029800027 PM 27309357 ER PT J AU Comstock, SS Maleki, SJ Teuber, SS AF Comstock, Sarah S. Maleki, Soheila J. Teuber, Suzanne S. TI Boiling and Frying Peanuts Decreases Soluble Peanut (Arachis Hypogaea) Allergens Ara h 1 and Ara h 2 But Does Not Generate Hypoallergenic Peanuts SO PLOS ONE LA English DT Article ID CROSS-REACTIVITY; ATOPIC-DERMATITIS; COOKING METHODS; PHAGE DISPLAY; IGE-BINDING; CASHEW NUT; PROTEINS; IDENTIFICATION; CHALLENGE; TOLERANCE AB Peanut allergy continues to be a problem in most developed countries of the world. We sought a processing method that would alter allergenic peanut proteins, such that allergen recognition by IgE from allergic individuals would be significantly reduced or eliminated. Such a method would render accidental exposures to trace amounts of peanuts safer. A combination of boiling and frying decreased recovery of Ara h 1 and Ara h 2 at their expected MWs. In contrast, treatment with high pressures under varying temperatures had no effect on protein extraction profiles. Antibodies specific for Ara h 1, Ara h 2, and Ara h 6 bound proteins extracted from raw samples but not in boiled/fried samples. However, pre-incubation of serum with boiled/fried extract removed most raw peanut-reactive IgE from solution, including IgE directed to Ara h 1 and 2. Thus, this method of processing is unlikely to generate a peanut product tolerated by peanut allergic patients. Importantly, variability in individual patients' IgE repertoires may mean that some patients' IgE would bind fewer polypeptides in the sequentially processed seed. C1 [Comstock, Sarah S.; Teuber, Suzanne S.] Univ Calif Davis, Dept Internal Med, Div Rheumatol Allergy & Clin Immunol, Sch Med, Davis, CA 95616 USA. [Maleki, Soheila J.] USDA ARS SRRC, New Orleans, LA USA. [Comstock, Sarah S.] Michigan State Univ, Dept Food Sci & Human Nutr, E Lansing, MI 48824 USA. RP Comstock, SS (reprint author), Univ Calif Davis, Dept Internal Med, Div Rheumatol Allergy & Clin Immunol, Sch Med, Davis, CA 95616 USA.; Comstock, SS (reprint author), Michigan State Univ, Dept Food Sci & Human Nutr, E Lansing, MI 48824 USA. EM comsto37@msu.edu FU Masterfoods Inc.; University of California, Davis FX This research was partially supported by Masterfoods Inc. (SST). The remaining funding was provided by the University of California, Davis. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 47 TC 0 Z9 0 U1 7 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 16 PY 2016 VL 11 IS 6 AR e0157849 DI 10.1371/journal.pone.0157849 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DO8JO UT WOS:000378029800139 PM 27310538 ER PT J AU Hiebert, CW Kolmer, JA McCartney, CA Briggs, J Fetch, T Bariana, H Choulet, F Rouse, MN Spielmeyer, W AF Hiebert, Colin W. Kolmer, James A. McCartney, Curt A. Briggs, Jordan Fetch, Tom Bariana, Harbans Choulet, Frederic Rouse, Matthew N. Spielmeyer, Wolfgang TI Major Gene for Field Stem Rust Resistance Co-Locates with Resistance Gene Sr12 in 'Thatcher' Wheat SO PLOS ONE LA English DT Article ID ADULT-PLANT RESISTANCE; TRITICUM-AESTIVUM L.; F-SP TRITICI; RED SPRING WHEAT; LEAF RUST; BREAD WHEAT; CHROMOSOME 7DL; COMMON WHEAT; RAPID METHOD; RACE TTKS AB Stem rust, caused by Puccinia graminis (Pgt), is a damaging disease of wheat that can be controlled by utilizing effective stem rust resistance genes. ` Thatcher' wheat carries complex resistance to stem rust that is enhanced in the presence of the resistance gene Lr34. The purpose of this study was to examine APR in ` Thatcher' and look for genetic interactions with Lr34. A RIL population was tested for stem rust resistance in field nurseries in Canada, USA, and Kenya. BSA was used to find SNP markers associated with reduced stem rust severity. A major QTL was identified on chromosome 3BL near the centromere in all environments. Seedling testing showed that Sr12 mapped to the same region as the QTL for APR. The SNP markers were physically mapped and the region carrying the resistance was searched for sequences with homology to members of the NB-LRR resistance gene family. SNP marker from one NB-LRR-like sequence, NB-LRR3 co-segregated with Sr12. Two additional populations, including one that lacked Lr34, were tested in field nurseries. NB-LRR3 mapped near the maximum LOD for reduction in stem rust severity in both populations. Lines from a population that segregated for Sr12 and Lr34 were tested for seedling Pgt biomass and infection type, as well as APR to field stem rust which showed an interaction between the genes. We concluded that Sr12, or a gene closely linked to Sr12, was responsible for ` Thatcher'-derived APR in several environments and this resistance was enhanced in the presence of Lr34. C1 [Hiebert, Colin W.; McCartney, Curt A.] Agr & Agri Food Canada, Cereal Res Ctr, Morden, MB, Canada. [Kolmer, James A.; Briggs, Jordan; Rouse, Matthew N.] Univ Minnesota, Dept Plant Pathol, St Paul, MN USA. [Kolmer, James A.; Rouse, Matthew N.] USDA ARS, Cereal Dis Lab, St Paul, MN USA. [Fetch, Tom] Agr & Agri Food Canada, Brandon Res Ctr, Brandon, MB, Canada. [Bariana, Harbans] Univ Sydney, Plant Breeding Inst, Cobbitty, NSW, Australia. [Choulet, Frederic] INRA UMR1095 Genet Divers & Ecophysiol Cereals, Clermont Ferrand, France. [Spielmeyer, Wolfgang] CSIRO, Agr & Food, Canberra, ACT, Australia. RP Spielmeyer, W (reprint author), CSIRO, Agr & Food, Canberra, ACT, Australia. EM Wolfgang.Spielmeyer@csiro.au RI Rouse, Matthew/G-5474-2011 FU USDA-ARS Appropriated Project [5062-21220-021-00]; USDA-ARS National Plant Disease Recovery System; Durable Rust Resistance in Wheat project; Saskatchewan Agriculture Development Fund [20080009]; Agriculture and Agri-Food Canada Growing Forward; Genome Canada; Saskatchewan Ministry of Agriculture,; Western Grain Research Foundation FX The authors thank USDA-ARS Appropriated Project 5062-21220-021-00, USDA-ARS National Plant Disease Recovery System, the Durable Rust Resistance in Wheat project (MNR), Saskatchewan Agriculture Development Fund project #20080009 (CAM), Agriculture and Agri-Food Canada Growing Forward (CWH, TF, CAM), and as part of CTAG, a Genome Prairie project funded by Genome Canada, Saskatchewan Ministry of Agriculture, and Western Grain Research Foundation (CAM) for financial support. This project was also supported through a grant CIM/2007/084 provided by Australian Centre of International Agricultural Research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; The authors thank Mira Popovic, Ghassan Mardli, Tobi Malasiuk, Elaine Martineau, Taye Zegeye, Alison Morgan, Jessica Hyles, and Shideh Mojerlou for excellent technical assistance. The authors thank USDA-ARS Appropriated Project 5062-21220-021-00, USDA-ARS National Plant Disease Recovery System, the Durable Rust Resistance in Wheat project (MNR), Saskatchewan Agriculture Development Fund project #20080009 (CAM), Agriculture and Agri-Food Canada Growing Forward (CWH, TF, CAM), and as part of CTAG, a Genome Prairie project funded by Genome Canada, Saskatchewan Ministry of Agriculture, and Western Grain Research Foundation (CAM) for financial support. This project was also supported through a grant CIM/2007/084 provided by Australian Centre of International Agricultural Research. We also thank Dr Sridhar Bhavani for providing access to stem rust nursery at Njoro, Kenya. NR 49 TC 0 Z9 0 U1 4 U2 11 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 16 PY 2016 VL 11 IS 6 AR e0157029 DI 10.1371/journal.pone.0157029 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DO8JO UT WOS:000378029800029 PM 27309724 ER PT J AU Ge, LQ Xia, T Huang, B Song, QS Zhang, HW Stanley, D Yang, GQ Wu, JC AF Ge, Lin-Quan Xia, Ting Huang, Bo Song, Qi-Sheng Zhang, Hong-Wei Stanley, David Yang, Guo-Qing Wu, Jin-Cai TI Suppressing male spermatogenesis-associated protein 5-like gene expression reduces vitellogenin gene expression and fecundity in Nilaparvata lugens Stal SO SCIENTIFIC REPORTS LA English DT Article ID ACCESSORY-GLAND SUBSTANCES; HEMIPTERA DELPHACIDAE; BROWN PLANTHOPPER; ADULT FEMALES; HOMOPTERA; IDENTIFICATION; SPATA5; DIET AB In our previous study with the brown planthopper (BPH), Nilaparvata lugens, triazophos (tzp) treatments led to substantial up-regulation of a male spermatogenesis-associated protein 5-like gene (NlSPATA5) compared to untreated controls. Mating with tzp-treated males significantly increased fecundity (as numbers of eggs laid), relative to females mated with untreated males. Because SPATA5 acts in mammalian sperm development and is expressed in testes, we posed the hypothesis that NlSPATA5 occurs in BPH seminal fluid and it operates in fecundity via mating. We tested the hypothesis by investigating the influence of suppressing NlSPATA5 expression in BPH males on fecundity. Reduced expression of NlSPATA5 led to decreased male accessory gland protein content and reproductive system development compared to controls. These changes in males led to prolonged pre-oviposition periods and decreased fecundity in females. For both genders, we recorded no difference in the body weight, oviposition periods, and longevity compared to controls. NlSPATA5 suppression in males also led to decreased fat body and ovarian protein content, yeast-like symbionts abundance and ovarian development as well as vitellogenin gene expression in their mating partners. We infer that increased NlSPATA5 expression may be one molecular mechanism of tzp-driven reproduction and population increases in BPH. C1 [Ge, Lin-Quan; Xia, Ting; Huang, Bo; Yang, Guo-Qing; Wu, Jin-Cai] Yangzhou Univ, Sch Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China. [Song, Qi-Sheng; Zhang, Hong-Wei] Univ Missouri, Div Plant Sci, 1-31 Agr Bldg, Columbia, MO 65211 USA. [Stanley, David] ARS, USDA, Biol Control Insects Res Lab, Columbia, MO USA. RP Ge, LQ; Wu, JC (reprint author), Yangzhou Univ, Sch Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China. EM lqge1027@163.com; jincaiwu1952@sina.com FU National Natural Science Foundation of China [31201507]; Natural Science Foundation of Yangzhou [YZ2014039]; Science and Technology innovation of Foundation of Yangzhou University [2015CXJ044]; Natural Science Foundation of the Jiangsu Higher Education Institutions of China [12KJB210006] FX This research was supported in part by the National Natural Science Foundation of China (31201507), by the Natural Science Foundation of Yangzhou (YZ2014039), by the Science and Technology innovation of Foundation of Yangzhou University (2015CXJ044), by the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (12KJB210006). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US. Department of Agriculture. NR 34 TC 0 Z9 0 U1 11 U2 19 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUN 16 PY 2016 VL 6 AR 28111 DI 10.1038/srep28111 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DO9KM UT WOS:000378105200002 PM 27305948 ER PT J AU Thitaram, SN Frank, JF Siragusa, GR Bailey, JS Dargatz, DA Lombard, JE Haley, CA Lyon, SA Fedorka-Cray, PJ AF Thitaram, S. N. Frank, J. F. Siragusa, G. R. Bailey, J. S. Dargatz, D. A. Lombard, J. E. Haley, C. A. Lyon, S. A. Fedorka-Cray, P. J. TI Antimicrobial susceptibility of Clostridium difficile isolated from food animals on farms SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Antimicrobial susceptibility; Clostridium difficile; Swine; Dairy cattle; Beef cattle ID AGAR DILUTION METHOD; IN-VITRO ACTIVITY; ANAEROBIC-BACTERIA; MULTIINSTITUTIONAL OUTBREAK; REDUCED SUSCEPTIBILITY; ANTIBIOTIC-RESISTANCE; MEDICAL-CENTER; PCR RIBOTYPES; RISK-FACTORS; DIARRHEA AB Clostridium difficile is commonly associated with a spectrum of disease in humans referred to as C. difficile-associated disease (CDAD) and use of antimicrobials is considered a risk factor for development of disease in humans. C difficile can also inhabit healthy food animals and transmission to humans is possible. As a result of the complexity and cost of testing, C difficile is rarely tested for antimicrobial susceptibility. A total of 376 C difficile strains (94 each from swine and dairy feces, and 188 from beef cattle feces) were isolated from healthy food animals on farms during studies conducted by the National Animal Health Monitoring System. Using the Etest (AB Biodisk, Solna, Sweden), samples were tested for susceptibility to nine antimicrobials implicated as risk factors for CDAD (linezolid, amoxicillin-clavulanic acid, ampicillin, clindamycin, erythromycin, levofloxacin, metronidazole, rifampicin, and vancomycin). Vancomycin was active against all isolates of C difficile (MIC90 = 3.0 mu g/ml) while almost all isolates (n = 369; 98.1%) were resistant to levofloxacin. With the exception of vancomycin, resistance varied by animal species as follows: linezolid (8.5% resistance among swine versus 2.1 and 1.1% resistance among dairy and beef, respectively), clindamycin (56.4% resistance among swine versus 80% and 90.9% resistance among dairy and beef, respectively), and rifampicin (2.1% and 0% resistance among swine and dairy cattle isolates, respectively versus 143% resistance among beef isolates). Regardless of species, multiple drug resistance was observed most often to combinations of clindamycin and levofloxacin (n = 195; 51.9%) and ampicillin, clindamycin and levofloxacin (n = 41; 10.9%). The reason for the variability of resistance between animal species is unknown and requires further research. Published by Elsevier B.V. C1 [Thitaram, S. N.; Bailey, J. S.; Lyon, S. A.; Fedorka-Cray, P. J.] ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, USDA, 950 Coll Stn Rd, Athens, GA 30605 USA. [Frank, J. F.] Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA. [Siragusa, G. R.] ARS, Poultry Microbiol Safety & Proc Res Unit, USDA, 950 Coll Stn Rd, Athens, GA 30605 USA. Anim & Plant Hlth Inspect Serv, Ctr Epidemiol & Anim Hlth, USDA, 2150 Ctr Ave,Bldg B, Ft Collins, CO 80526 USA. [Siragusa, G. R.] Eurofins Microbiol Labs Inc, 2345 South 10th St, New Berlin, WI 53151 USA. [Bailey, J. S.] BioMerieux Ind Inc, 595 Anglum Rd, Hazelwood, MO 63042 USA. [Lyon, S. A.] Chick Fil A Inc, 5200 Buffington Rd, Atlanta, GA 30349 USA. [Fedorka-Cray, P. J.] N Carolina State Univ, Populat Hlth & Pathobiol Dept, 1060 William Moore Dr, Raleigh, NC 27607 USA. RP Thitaram, SN (reprint author), Food Safety & Inspect Serv, USDA, 950 Coll Stn Rd, Athens, GA 30605 USA. EM sutawee.thitaram@fsis.usda.gov NR 76 TC 3 Z9 3 U1 7 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD JUN 16 PY 2016 VL 227 BP 1 EP 5 DI 10.1016/j.ijfoodmicro.2016.03.017 PG 5 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA DM7NF UT WOS:000376546600001 PM 27043382 ER PT J AU Cote, GL Skory, CD AF Cote, Gregory L. Skory, Christopher D. TI Effect of a single point mutation on the interaction of glucans with a glucansucrase from Leuconostoc mesenteroides NRRL B-1118 SO CARBOHYDRATE RESEARCH LA English DT Article DE Dextran; Dextransucrase; Glucansucrase; Glucosyltransferase; Glucan-binding domain ID STREPTOCOCCUS-MUTANS DEXTRANSUCRASE; GLUCOSYLTRANSFERASE GTF-I; PRIMER DEXTRAN; ORAL STREPTOCOCCI; ACCEPTOR REACTION; MECHANISM; SOBRINUS; REQUIREMENT; EXPRESSION; SUCROSE AB Our previous work showed that substitution of an amino acid that is coupled with the +2 subsite adjacent to the transition stabilizer of a glucansucrase, which produces a water-insoluble glucan, resulted in significant changes in the structures and yields of the water-insoluble glucans produced. We now describe how these changes affect the ability of the glucansucrase to bind to exogenous glucans, and how these glucans can influence the yield, product structures, and kinetics of the mutant glucansucrases. The activity of the wild-type enzyme, with threonine at position 654, is not significantly activated by added dextran, and the yield of water-insoluble glucan from sucrose is only slightly increased by dextran. Mutant T654Y is not affected at all by the addition of dextran. However, several mutant enzymes exhibit markedly lower yields of glucan relative to the wild type; these lower yields can be partially or completely overcome by the addition of water-soluble dextran. Although evidence indicates that the soluble dextran is incorporated into water-insoluble glucan, the increased yields cannot be accounted for solely by incorporation of the dextran into insoluble product. Furthermore, these DsrI mutants are significantly activated by exogenous glucans. The addition of dextran does not markedly change the K-M for sucrose in the mutant enzymes, but does increase the V-max of the reaction. These effects apparently depend on the presence of unbranched sequences of alpha 1 -> 6-linked D-glucose units in the glucan. Published by Elsevier Ltd. C1 [Cote, Gregory L.; Skory, Christopher D.] ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. RP Cote, GL (reprint author), ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM greg.cote@ars.usda.gov FU U.S. Department of Agriculture FX We thank Suzanne Unser and Kristina Glenzinski for their many hours of technical assistance, Dr. Karl Vermillion for his expert advice and assistance pertaining to NMR spectroscopy, and Dr. Joseph Rich for his support and encouragement of our work. This work was supported and carried out by the U.S. Department of Agriculture. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 36 TC 0 Z9 0 U1 5 U2 8 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0008-6215 EI 1873-426X J9 CARBOHYD RES JI Carbohydr. Res. PD JUN 16 PY 2016 VL 428 BP 57 EP 61 DI 10.1016/j.carres.2016.04.010 PG 5 WC Biochemistry & Molecular Biology; Chemistry, Applied; Chemistry, Organic SC Biochemistry & Molecular Biology; Chemistry GA DM3RD UT WOS:000376263000007 PM 27131127 ER PT J AU Llorens, F Thune, K Schmitz, M Ansoleaga, B Frau-Mendez, MA Cramm, M Tahir, W Gotzmann, N Berjaoui, S Carmona, M Silva, CJ Fernandez-Vega, I Zarranz, JJ Zerr, I Ferrer, I AF Llorens, Franc Thuene, Katrin Schmitz, Matthias Ansoleaga, Belen Frau-Mendez, Margalida A. Cramm, Maria Tahir, Waqas Gotzmann, Nadine Berjaoui, Sara Carmona, Margarita Silva, Christopher J. Fernandez-Vega, Ivan Jose Zarranz, Juan Zerr, Inga Ferrer, Isidro TI Identification of new molecular alterations in fatal familial insomnia SO HUMAN MOLECULAR GENETICS LA English DT Article ID CREUTZFELDT-JAKOB-DISEASE; QUAKING-INDUCED CONVERSION; RESISTANT PRION PROTEIN; GENE-EXPRESSION PROFILES; CEREBROSPINAL-FLUID; CLINICAL-FEATURES; BRAIN; MUTATION; PRPSC; GLYCOSYLATION AB Fatal familial insomnia is a rare disease caused by a D178N mutation in combination with methionine (Met) at codon 129 in the mutated allele of PRNP (D178N-129M haplotype). FFI is manifested by sleep disturbances with insomnia, autonomic disorders and spontaneous and evoked myoclonus, among other symptoms. This study describes new neuropathological and biochemical observations in a series of eight patients with FFI. The mediodorsal and anterior nuclei of the thalamus have severe neuronal loss and marked astrocytic gliosis in every case, whereas the entorhinal cortex is variably affected. Spongiform degeneration only occurs in the entorhinal cortex. Synaptic and fine granular proteinase K digestion (PrPres) immunoreactivity is found in the entorhinal cortex but not in the thalamus. Interleukin 6, interleukin 10 receptor alpha subunit, colony stimulating factor 3 receptor and toll-like receptor 7 mRNA expression increases in the thalamus in FFI. PrPc levels are significantly decreased in the thalamus, entorhinal cortex and cerebellum in FFI. This is accompanied by a particular PrPc and PrPres band profile. Altered PrP solubility consistent with significantly reduced PrP levels in the cytoplasmic fraction and increased PrP levels in the insoluble fraction are identified in FFI cases. Amyloid-like deposits are only seen in the entorhinal cortex. The RT-QuIC assay reveals that all the FFI samples of the entorhinal cortex are positive, whereas the thalamus is positive only in three cases and the cerebellumin two cases. The present findings unveil particular neuropathological and neuroinflammatory profiles in FFI and novel characteristics of natural prion protein in FFI, altered PrPres and Scrapie PrP (abnormal and pathogenic PrP) patterns and region-dependent putative capacity of PrP seeding. C1 [Llorens, Franc; Thuene, Katrin; Schmitz, Matthias; Cramm, Maria; Tahir, Waqas; Gotzmann, Nadine; Zerr, Inga] Univ Med Ctr Gottingen, Dept Neurol, D-37075 Gottingen, Germany. [Llorens, Franc; Thuene, Katrin; Schmitz, Matthias; Cramm, Maria; Tahir, Waqas; Gotzmann, Nadine; Zerr, Inga] German Ctr Neurodegenerat Dis DZNE Site Gottingen, D-37075 Gottingen, Germany. [Ansoleaga, Belen; Frau-Mendez, Margalida A.; Berjaoui, Sara; Carmona, Margarita; Ferrer, Isidro] Univ Barcelona, Bellvitge Biomed Res Inst IDIBELL, Bellvitge Univ Hosp, Inst Neuropathol,Serv Pathol Anat, Lhospitalet De Llobregat, Spain. [Ansoleaga, Belen; Frau-Mendez, Margalida A.; Berjaoui, Sara; Carmona, Margarita; Ferrer, Isidro] Biomed Res Ctr Neurodegenerat Dis CIBERNED, Lhospitalet De Llobregat 08907, Spain. [Silva, Christopher J.] USDA, Produce Safety & Microbiol Res Unit, Western Reg Res Ctr, Albany, CA 94710 USA. [Fernandez-Vega, Ivan] Univ Hosp Araba, Dept Pathol, Alava 01009, Spain. [Fernandez-Vega, Ivan] Araba Univ Hosp, Brain Bank, Basque Biobank Res O eHun, Alava 01009, Spain. [Jose Zarranz, Juan] Univ Basque Country, Univ Hosp Cruces, Dept Neurol, Bizkaia 48903, Spain. RP Ferrer, I (reprint author), Bellvitge Univ Hosp, Serv Anat Pathol, Inst Neuropathol, Carrer Feixa Llarga Sn, Lhospitalet De Llobregat 08907, Spain. EM 8082ifa@gmail.com FU European Commission [278486, 222887, FP7-KBBE-2007-2A]; Spanish Ministry of Health, Instituto Carlos III (Fondo de Investigacion Sanitaria [FIS] [PI1100968, FIS PI14/00757]; CIBERNED project BESAD-P; Neurodegenerative Disease Research (JPND-DEMTEST: Biomarker based diagnosis of rapidly progressive dementias-optimization of diagnostic protocols) [01ED1201A]; Red Nacional de priones [AGL2015-71764-REDT- MINECO] FX This study was supported by the Seventh Framework Program of the European Commission (Grant No. 278486 [DEVELAGE project]), the Spanish Ministry of Health, Instituto Carlos III (Fondo de Investigacion Sanitaria [FIS] PI1100968, FIS PI14/00757 and CIBERNED project BESAD-P to I.F.), the European Commission: Protecting the food chain from prions: shaping European priorities through basic and applied research (PRIORITY, No. 222887) Project number: FP7-KBBE-2007-2A, the Neurodegenerative Disease Research (JPND-DEMTEST: Biomarker based diagnosis of rapidly progressive dementias-optimization of diagnostic protocols, 01ED1201A) to I.Z. and the Red Nacional de priones (AGL2015-71764-REDT- MINECO) to I.F., F.L. and I.Z. NR 59 TC 0 Z9 0 U1 3 U2 3 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0964-6906 EI 1460-2083 J9 HUM MOL GENET JI Hum. Mol. Genet. PD JUN 15 PY 2016 VL 25 IS 12 BP 2417 EP 2436 DI 10.1093/hmg/ddw108 PG 20 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA EJ2TA UT WOS:000393062900005 PM 27056979 ER PT J AU He, D Kuhn, D Parida, L AF He, Dan Kuhn, David Parida, Laxmi TI Novel applications of multitask learning and multiple output regression to multiple genetic trait prediction SO BIOINFORMATICS LA English DT Article; Proceedings Paper CT 24th Annual Conference on Intelligent Systems for Molecular Biology (ISMB) CY JUL 08-12, 2016 CL Orlando, FL ID MARKER-ASSISTED SELECTION; GENOMIC SELECTION; QUANTITATIVE TRAITS; RIDGE-REGRESSION; IMPROVEMENT; ACCURACY; LASSO AB Given a set of biallelic molecular markers, such as SNPs, with genotype values encoded numerically on a collection of plant, animal or human samples, the goal of genetic trait prediction is to predict the quantitative trait values by simultaneously modeling all marker effects. Genetic trait prediction is usually represented as linear regression models. In many cases, for the same set of samples and markers, multiple traits are observed. Some of these traits might be correlated with each other. Therefore, modeling all the multiple traits together may improve the prediction accuracy. In this work, we view the multitrait prediction problem from a machine learning angle: as either a multitask learning problem or a multiple output regression problem, depending on whether different traits share the same genotype matrix or not. We then adapted multitask learning algorithms and multiple output regression algorithms to solve the multitrait prediction problem. We proposed a few strategies to improve the least square error of the prediction from these algorithms. Our experiments show thatmodelingmultiple traits together could improve the prediction accuracy for correlated traits. C1 [He, Dan; Parida, Laxmi] IBM TJ Watson Res, Yorktown Hts, NY 10598 USA. [Kuhn, David] USDA ARS, Subtrop Hort Res Stn, 13601 Old Cutler Rd, Miami, FL 33158 USA. RP He, D (reprint author), IBM TJ Watson Res, Yorktown Hts, NY 10598 USA. EM dhe@us.ibm.com NR 32 TC 1 Z9 1 U1 1 U2 2 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1367-4803 EI 1460-2059 J9 BIOINFORMATICS JI Bioinformatics PD JUN 15 PY 2016 VL 32 IS 12 BP 37 EP 43 DI 10.1093/bioinformatics/btw249 PG 7 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Computer Science, Interdisciplinary Applications; Mathematical & Computational Biology; Statistics & Probability SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Computer Science; Mathematical & Computational Biology; Mathematics GA DR2KV UT WOS:000379734300005 ER PT J AU Chagas, BME Mullen, CA Dorado, C Elkasabi, Y Boateng, AA Melo, MAF Ataide, CH AF Chagas, Bruna M. E. Mullen, Charles A. Dorado, Christina Elkasabi, Yaseen Boateng, Akwasi A. Melo, Marcus A. F. Ataide, Carlos H. TI Stable Bio-oil Production from Proteinaceous Cyanobacteria: Tail Gas Reactive Pyrolysis of Spirulina SO INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH LA English DT Article ID PARTHENIUM-ARGENTATUM PYROLYSIS; LOW-LIPID MICROALGAE; FLUIDIZED-BED; LIGNOCELLULOSIC BIOMASS; CATALYTIC PYROLYSIS; SEWAGE-SLUDGE; LIQUEFACTION; FUELS; ARTHROSPIRA; AROMATICS AB Pyrolysis of spirulina, a cyanobacteria with high levels of protein (74 wt %) and low levels of lipid (0.8 wt %) content, has the potential to produce fuels and platform chemicals that differ from those produced from lignocellulosic materials. The yields and product distribution from fluidized-bed pyrolysis of spirulina using the U.S. Department of Agriculture - Agricultural Research Service's tail gas reactive pyrolysis (TGRP) process were evaluated and compared with those produced under an inert atmosphere. Important differences include improved performance of the system when using TGRP along with lower viscosity (77.6-148.5 cP at 27 degrees C) and higher energy content (32.5-33.5 MJ/kg) of the bio-oil compared to conventionally produced liquids. Chemically, the TGRP bio-oils were composed largely of aromatic hydrocarbons, phenols, and nitrogenated compounds. This more stable mixture allowed for distillation of the bio-oil into fractions with higher concentrations of certain platform chemicals including phenolics and nitrogenated compounds such as pyrrole. In addition to the liquid, the biochar and noncondensable gases had improved properties. C1 [Chagas, Bruna M. E.; Melo, Marcus A. F.] Univ Fed Rio Grande do Norte, Dept Engn Quim, BR-59078970 Natal, RN, Brazil. [Chagas, Bruna M. E.; Mullen, Charles A.; Dorado, Christina; Elkasabi, Yaseen; Boateng, Akwasi A.] USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Ataide, Carlos H.] Univ Fed Uberlandia, Fac Engn Quim, Campus Santa Monica,Bloco 1K, BR-38408144 Uberlandia, MG, Brazil. RP Boateng, AA (reprint author), USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM akwasi.boateng@ars.usda.gov FU Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (Capes); USDA-ARS/UFRN NFCA [8072-41000-095-24] FX This research was financially supported by Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (Capes) and performed under the USDA-ARS/UFRN NFCA 8072-41000-095-24. The authors acknowledge the technical support provided by Mr. Craig Einfeldt and thank Dr. Gary Strahan for acquiring and assisting in the analysis of the NMR spectra. NR 34 TC 1 Z9 1 U1 8 U2 10 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0888-5885 J9 IND ENG CHEM RES JI Ind. Eng. Chem. Res. PD JUN 15 PY 2016 VL 55 IS 23 BP 6734 EP 6741 DI 10.1021/acs.iecr.6b00490 PG 8 WC Engineering, Chemical SC Engineering GA DP0QL UT WOS:000378194200020 ER PT J AU Elder, T Berstis, L Beckham, GT Crowley, MF AF Elder, Thomas Berstis, Laura Beckham, Gregg T. Crowley, Michael F. TI Coupling and Reactions of 5-Hydroxyconiferyl Alcohol in Lignin Formation SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE 5-hydroxyconiferyl alcohol; coniferyl alcohol; lignin; benzodioxane; stereochemistry ID BOND-DISSOCIATION ENTHALPIES; PHENETHYL PHENYL ETHER; DENSITY-FUNCTIONAL THEORY; MODEL COMPOUNDS; ALPHA/BETA-SELECTIVITIES; COMPUTATIONAL PREDICTION; PYROLYSIS MECHANISM; BETA-O-4; POLYMERIZATION; BIOSYNTHESIS AB The catechol alcohols, caffeyl and 5-hydroxyconiferyl alcohol, may be incorporated into lignin either naturally or through genetic manipulation. Due to the presence of o-OH groups, these compounds form benzodioxanes, a departure from the interunit connections found in lignins derived from the cinnamyl alcohols. In nature, lignins composed of caffeyl and 5-hydroxyconiferyl alcohol are linear homopolymers and, as such, may have properties that make them amenable for use in value-added products, such as lignin-based carbon fibers. In the current work, results from density functional theory calculations for the reactions of 5-hydroxyconiferyl alcohol, taking stereochemistry into account, are reported. Dehydrogenation and quinone methide formation are found to be thermodynamically favored for 5-hydroxyconiferyl alcohol, over coniferyl alcohol. The comparative energetics of the rearomatization reactions suggest that the formation of the benzodioxane linkage is under kinetic control. Ring-opening reactions of the benzodioxane groups show that the bond dissociation enthalpy of the alpha-O cleavage reaction is lower than that of the beta-O reaction. The catechol lignins represent a novel form of the polymer that may offer new opportunities for bioproducts and genetic targets. C1 [Elder, Thomas] Forest Serv, USDA, Southern Res Stn, 521 Devall Dr, Auburn, AL 36849 USA. [Berstis, Laura; Beckham, Gregg T.] Natl Renewable Energy Lab, Nat Bioenergy Ctr, 15013 Denver West Pkwy, Golden, CO 80401 USA. [Crowley, Michael F.] Natl Renewable Energy Lab, Biosci Ctr, 15013 Denver West Pkwy, Golden, CO 80401 USA. RP Elder, T (reprint author), Forest Serv, USDA, Southern Res Stn, 521 Devall Dr, Auburn, AL 36849 USA. EM telder@fs.fed.us FU US Department of Energy, Bioenergy Technologies Office FX L.B., G.T.B., and M.F.C. acknowledge funding from the US Department of Energy, Bioenergy Technologies Office. NR 41 TC 0 Z9 0 U1 7 U2 9 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 15 PY 2016 VL 64 IS 23 BP 4742 EP 4750 DI 10.1021/acs.jafc.6b02234 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DP0QI UT WOS:000378193900005 PM 27236926 ER PT J AU Khoury, CK Achicanoy, HA Bjorkman, AD Navarro-Racines, C Guarino, L Flores-Palacios, X Engels, JMM Wiersema, JH Dempewolf, H Sotelo, S Ramirez-Villegas, J Castaneda-Alvarez, NP Fowler, C Jarvis, A Rieseberg, LH Struik, PC AF Khoury, Colin K. Achicanoy, Harold A. Bjorkman, Anne D. Navarro-Racines, Carlos Guarino, Luigi Flores-Palacios, Ximena Engels, Johannes M. M. Wiersema, John H. Dempewolf, Hannes Sotelo, Steven Ramirez-Villegas, Julian Castaneda-Alvarez, Nora P. Fowler, Cary Jarvis, Andy Rieseberg, Loren H. Struik, Paul C. TI Origins of food crops connect countries worldwide SO PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Article DE crop diversity; crop domestication; crop improvement; crop origins; food security; plant genetic resources ID PLANT GENETIC-RESOURCES; SECONDARY CENTER; DIVERSITY; SECURITY; CONSERVATION; PRODUCTIVITY; COLLECTION; CENTERS; TRADE AB Research into the origins of food plants has led to the recognition that specific geographical regions around the world have been of particular importance to the development of agricultural crops. Yet the relative contributions of these different regions in the context of current food systems have not been quantified. Here we determine the origins ('primary regions of diversity') of the crops comprising the food supplies and agricultural production of countries worldwide. We estimate the degree to which countries use crops from regions of diversity other than their own ('foreign crops'), and quantify changes in this usage over the past 50 years. Countries are highly interconnected with regard to primary regions of diversity of the crops they cultivate and/or consume. Foreign crops are extensively used in food supplies (68.7% of national food supplies as a global mean are derived from foreign crops) and production systems (69.3% of crops grown are foreign). Foreign crop usage has increased significantly over the past 50 years, including in countries with high indigenous crop diversity. The results provide a novel perspective on the ongoing globalization of food systems worldwide, and bolster evidence for the importance of international collaboration on genetic resource conservation and exchange. C1 [Khoury, Colin K.; Achicanoy, Harold A.; Navarro-Racines, Carlos; Sotelo, Steven; Ramirez-Villegas, Julian; Castaneda-Alvarez, Nora P.; Jarvis, Andy] Int Ctr Trop Agr CIAT, Km 17,Apartado Aereo 6713, Cali 763537, Colombia. [Khoury, Colin K.; Struik, Paul C.] Wageningen Univ, Ctr Crop Syst Anal, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands. [Khoury, Colin K.] ARS, USDA, Natl Lab Genet Resources Preservat, 1111 South Mason St, Ft Collins, CO 80521 USA. [Bjorkman, Anne D.] German Ctr Integrat Biodivers Res iDiv, Leipzig, Germany. [Bjorkman, Anne D.] Univ Edinburgh, Sch Geosci, James Hutton Rd, Edinburgh EH9 3FE, Midlothian, Scotland. [Navarro-Racines, Carlos; Ramirez-Villegas, Julian; Jarvis, Andy] CGIAR Res Program Climate Change Agr & Food Secur, Km 17,Apartado Aereo 6713, Cali 763537, Colombia. [Guarino, Luigi; Dempewolf, Hannes; Fowler, Cary] Global Crop Divers Trust, Pl Vereinten Nationen 7, D-53113 Bonn, Germany. [Flores-Palacios, Ximena] Auckland Univ Technol, 55 Wellesley St E, Auckland 1010, New Zealand. [Engels, Johannes M. M.] Biovers Int, Via Tre Denari 472-A, I-00057 Maccarese, Italy. [Wiersema, John H.] ARS, USDA, Natl Germplasm Res Lab, Bldg 003,BARC West, Beltsville, MD 20705 USA. [Ramirez-Villegas, Julian] Univ Leeds, Sch Earth & Environm, Leeds, W Yorkshire, England. [Castaneda-Alvarez, Nora P.] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England. [Rieseberg, Loren H.] Biodivers Res Ctr, Vancouver, BC V6T 1Z4, Canada. [Rieseberg, Loren H.] Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z4, Canada. [Rieseberg, Loren H.] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA. RP Khoury, CK (reprint author), Int Ctr Trop Agr CIAT, Km 17,Apartado Aereo 6713, Cali 763537, Colombia.; Khoury, CK (reprint author), Wageningen Univ, Ctr Crop Syst Anal, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands.; Khoury, CK (reprint author), ARS, USDA, Natl Lab Genet Resources Preservat, 1111 South Mason St, Ft Collins, CO 80521 USA. EM c.khoury@cgiar.org OI Khoury, Colin K./0000-0001-7893-5744; Rieseberg, Loren/0000-0002-2712-2417; Sotelo, Steven/0000-0002-9621-1456 FU 'Adapting Agriculture to Climate Change: Collecting, Protecting and Preparing Crop Wild Relatives' project - Government of Norway FX Financial support came from the 'Adapting Agriculture to Climate Change: Collecting, Protecting and Preparing Crop Wild Relatives' project, which is supported by the Government of Norway, managed by the Global Crop Diversity Trust with the Millennium Seed Bank of the Royal Botanic Gardens, Kew, UK, and implemented in partnership with national and international genebanks and plant breeding institutes around the world. For further information, see www.cwrdiversity.org. NR 53 TC 2 Z9 2 U1 12 U2 17 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8452 EI 1471-2954 J9 P ROY SOC B-BIOL SCI JI Proc. R. Soc. B-Biol. Sci. PD JUN 15 PY 2016 VL 283 IS 1832 AR 20160792 DI 10.1098/rspb.2016.0792 PG 9 WC Biology; Ecology; Evolutionary Biology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Evolutionary Biology GA DP2KZ UT WOS:000378318700021 ER PT J AU Walter, KS Pepin, KM Webb, CT Gaff, HD Krause, PJ Pitzer, VE Diuk-Wasser, MA AF Walter, Katharine S. Pepin, Kim M. Webb, Colleen T. Gaff, Holly D. Krause, Peter J. Pitzer, Virginia E. Diuk-Wasser, Maria A. TI Invasion of two tick-borne diseases across New England: harnessing human surveillance data to capture underlying ecological invasion processes SO PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Article DE invasion; Lyme disease; babesiosis; tick-borne disease; occupancy model ID VECTOR IXODES-SCAPULARIS; EASTERN UNITED-STATES; HUMAN LYME-DISEASE; BORRELIA-BURGDORFERI; HUMAN BABESIOSIS; RANGE EXPANSION; METAPOPULATION DYNAMICS; INFECTIOUS-DISEASE; NANTUCKET-ISLAND; DAMMINI AB Modelling the spatial spread of vector-borne zoonotic pathogens maintained in enzootic transmission cycles remains a major challenge. The best available spatio-temporal data on pathogen spread often take the form of human disease surveillance data. By applying a classic ecological approach-occupancy modelling-to an epidemiological question of disease spread, we used surveillance data to examine the latent ecological invasion of tick-borne pathogens. Over the last half-century, previously undescribed tick-borne pathogens including the agents of Lyme disease and human babesiosis have rapidly spread across the northeast United States. Despite their epidemiological importance, the mechanisms of tick-borne pathogen invasion and drivers underlying the distinct invasion trajectories of the co-vectored pathogens remain unresolved. Our approach allowed us to estimate the unobserved ecological processes underlying pathogen spread while accounting for imperfect detection of human cases. Our model predicts that tick-borne diseases spread in a diffusion-like manner with occasional long-distance dispersal and that babesiosis spread exhibits strong dependence on Lyme disease. C1 [Walter, Katharine S.; Krause, Peter J.; Pitzer, Virginia E.; Diuk-Wasser, Maria A.] Yale Univ, Sch Publ Hlth, Dept Epidemiol Microbial Dis, 60 Coll St, New Haven, CT 06520 USA. [Pepin, Kim M.] US Anim & Plant Hlth Inspect Serv, USDA, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. [Pepin, Kim M.; Webb, Colleen T.; Pitzer, Virginia E.] NIH, Fogarty Int Ctr, Bldg 10, Bethesda, MD 20892 USA. [Webb, Colleen T.] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Gaff, Holly D.] Old Dominion Univ, Dept Biol Sci, 302a Mills Godwin Bldg, Norfolk, VA 23529 USA. [Diuk-Wasser, Maria A.] Columbia Univ, Dept Ecol Evolut & Environm Biol, 1200 Amsterdam Ave, New York, NY 10027 USA. RP Walter, KS (reprint author), Yale Univ, Sch Publ Hlth, Dept Epidemiol Microbial Dis, 60 Coll St, New Haven, CT 06520 USA. EM katharine.walter@yale.edu OI Pitzer, Virginia/0000-0003-1015-2289 FU NIH pre-doctoral training grant [5T32AI007404-23]; NIH Ruth L. Kirschstein National Research Service Award [F31 AI118233-01A1]; Research and Policy in Disease Dynamics (RAPIDD) programme, Fogarty International Center, NIH; Department of Homeland Security; USDA-APHIS-WS; RAPIDD; Gordon and Laura Gund Foundation; National Institutes of Health, Ecology and Evolution of Infectious Disease Programme [R01 GM105246] FX K.S.W. was supported by the NIH pre-doctoral training grant 5T32AI007404-23 and the NIH Ruth L. Kirschstein National Research Service Award F31 AI118233-01A1. K.M.P. was partly supported by the Research and Policy in Disease Dynamics (RAPIDD) programme, Fogarty International Center, NIH and Department of Homeland Security and partly by USDA-APHIS-WS. C.T.W. and V.E.P. were funded by RAPIDD. P.J.K. was supported by the Gordon and Laura Gund Foundation. M.D.W. was supported by the National Institutes of Health, Ecology and Evolution of Infectious Disease Programme (R01 GM105246). NR 62 TC 0 Z9 0 U1 10 U2 21 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8452 EI 1471-2954 J9 P ROY SOC B-BIOL SCI JI Proc. R. Soc. B-Biol. Sci. PD JUN 15 PY 2016 VL 283 IS 1832 AR 20160834 DI 10.1098/rspb.2016.0834 PG 10 WC Biology; Ecology; Evolutionary Biology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Evolutionary Biology GA DP2KZ UT WOS:000378318700023 ER PT J AU Dubey, JP Calero-Bernal, R Verma, SK Mowery, JD AF Dubey, J. P. Calero-Bernal, R. Verma, S. K. Mowery, J. D. TI Pathology, immunohistochemistry, and ultrastructural findings associated with neurological sarcocystosis in cattle SO VETERINARY PARASITOLOGY LA English DT Article DE Sarcocystosis; Cattle; Meningoencephalitis; Neurons; Schizonts ID ENCEPHALITIS AB Paraffin-embedded blocks of brain of a nine months old bull calf that died of neurological signs in 1982 in Germany were restudied. Numerous schizonts and merozoites were found associated with extensive but focal necrosis and severe meningoencephalitis. Developing stages of schizonts as well as free merozoites were identified. The schizonts were primarily in perivascular areas. Ultrastructurally, schizonts were seen both in capillaries and in extravascular space. Merozoites were often concentrated in adventitial layers of capillaries. Schizonts divided by endopolygeny, the nucleus became multi-lobed, and at the terminal stage nuclear lobes were incorporated into budding merozoites. Individual merozoites were seen in neurons, astrocytes, oligodendrocytes, leukocytes, and vascular endothelial cells. Occasionally merozoites were present in the nucleus of mononuclear cells. Individual merozoites were ovoid, 3-5 x 2-3 mu m in size, and contained a prominent nucleus, numerous micronemes, a conoid, but no rhoptries. Schizonts and merozoites did not react to polyclonal rabbit Neospora caninum, Toxoplasma gondii, and Sarcocystis neurona antibodies but did react to Sarcocystis cruzi antibodies. Because of morphological characteristics and the type of lesions, the parasite was likely due to an unidentified Sarcocystis species, different from S. cruzi. Published by Elsevier B.V. C1 [Dubey, J. P.; Calero-Bernal, R.; Verma, S. K.] ARS, USDA, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, Bldg 1001, Beltsville, MD 20705 USA. [Mowery, J. D.] ARS, USDA, Electron & Confocal Microscopy Unit, Beltsville Agr Res Ctr, Bldg 12, Beltsville, MD 20705 USA. RP Dubey, JP (reprint author), ARS, USDA, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, Bldg 1001, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov NR 14 TC 0 Z9 0 U1 2 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD JUN 15 PY 2016 VL 223 BP 147 EP 152 DI 10.1016/j.vetpar.2016.04.025 PG 6 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA DN8GM UT WOS:000377317200026 PM 27198793 ER PT J AU Zhang, YL Song, CH Sun, G Band, LE McNulty, S Noormets, A Zhang, QF Zhang, ZQ AF Zhang, Yulong Song, Conghe Sun, Ge Band, Lawrence E. McNulty, Steven Noormets, Asko Zhang, Quanfa Zhang, Zhiqiang TI Development of a coupled carbon and water model for estimating global gross primary productivity and evapotranspiration based on eddy flux and remote sensing data SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Gross primary productivity; Evapotranspiration; Light-use efficiency; Water-use efficiency; FLUXNET; MODIS; CCW ID LIGHT-USE EFFICIENCY; NET PRIMARY PRODUCTIVITY; ENERGY-BALANCE CLOSURE; TERRESTRIAL VEGETATION; DIFFUSE-RADIATION; CLIMATE; SATELLITE; FOREST; CO2; BIOSPHERE AB Terrestrial gross primary productivity (GPP) and evapotranspiration (ET) are two key ecosystem fluxes in the global carbon and water cycles. As carbon and water fluxes are inherently linked, knowing one provides information for the other. However, tightly coupled and easy to use ecosystem models are rare and there are still large uncertainties in global carbon and water flux estimates. In this study, we developed a new monthly coupled carbon and water (CCW) model. GPP was estimated based on the light-use efficiency (LUE) theory that considered the effect of diffuse radiation, while ET was modeled based on GPP and water-use efficiency (WUE). We evaluated the non-linear effect of single (GPP(OR)) or combined (GPP(AND)) limitations of temperature and vapor pressure deficit on GPP. We further compared the effects of three types of WUE (i.e., WUE, inherent WUE, and underlying WUE) on ET (i.e., ETWUE, E-IWUE and ETUWUE). CCW was calibrated and validated using global eddy covariance measurement from FLUXNET and remote sensing data from Moderate Resolution Imaging Spectroradiometer (MODIS) from 2000 to 2007. Modeled GPP(AND) and GPP(OR) explained 67.3% and 66.8% of variations of tower-derived GPP, respectively, while ETUWUE, E-IWUE and ETWUE explained 65.7%, 59.9% and 58.1% of tower-measured ET, respectively. Consequently, we chose GPPAND and ETuwuE as the best modeling framework for CCW, and estimated global GPP as 134.2 Pg Cyr(-1) and ET as 57.0 x 10(3) km(3) for vegetated areas in 2001. Global ET estimated by CCW compared favorably with MODIS ET (60.5 x 10(3) km(3)) and ET derived from global precipitation (56.5 x 10(3) km(3)). However, global GPP estimated by CCW was about 19% higher than MODIS GPP (109.0 Pg C yr(-1)). The mean global WUE value estimated by CCW (2.35 g C kg(-1) H2O) was close to the mean tower-based WUE (2.60 g C kg(-1) H2O), but was much higher than the WUE derived from MODIS products (1.80 g C kg(-1) H2O). We concluded that the new simple CCW model provided improved estimates of GPP and ET. The biome-specific parameters derived in this study allow CCW to be further linked with land use change models to project human impacts on terrestrial ecosystem functions. (C) 2016 Elsevier B.V. All rights reserved. C1 [Zhang, Yulong; Song, Conghe; Band, Lawrence E.] Univ N Carolina, Dept Geog, Chapel Hill, NC 27599 USA. [Zhang, Yulong; Band, Lawrence E.] Univ N Carolina, Inst Environm, Chapel Hill, NC 27599 USA. [Song, Conghe] E China Normal Univ, Sch Ecol & Environm Sci, Shanghai 200241, Peoples R China. [Sun, Ge; McNulty, Steven; Noormets, Asko] USDA Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Southern Res Stn, Raleigh, NC 27606 USA. [Zhang, Quanfa] Chinese Acad Sci, Wuhan Bot Garden, Key Lab Aquat Bot & Watershed Ecol, Wuhan 430074, Peoples R China. [Zhang, Zhiqiang] Beijing Forestry Univ, Sch Soil & Water Conservat, Beijing 100083, Peoples R China. RP Zhang, YL; Song, CH (reprint author), Univ N Carolina, Dept Geog, Chapel Hill, NC 27599 USA. EM ylzhang@unc.edu; csong@email.unc.edu RI Zhang, Yulong/K-1104-2015; Zhang, Quanfa/H-1922-2011 OI Zhang, Yulong/0000-0002-4263-8067; FU UNC-USDA Forest Service [14-JV-11330110-045]; National Science Foundation of U.S. [DEB-1313756]; Natural Science Foundation of China [31528004] FX Carboafrica, Koflux, NECC, TCOS-Siberia and AfriFlux (http://www.fluxdata.org/). We appreciate the flux tower Pis who made these data freely available and all the other people who were involved in the tower field work. This work was financially supported by the UNC-USDA Forest Service Joint Venture Agreement (14-JV-11330110-045), National Science Foundation of U.S. (DEB-1313756) and Natural Science Foundation of China (31528004). NR 90 TC 2 Z9 2 U1 25 U2 50 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD JUN 15 PY 2016 VL 223 BP 116 EP 131 DI 10.1016/j.agrformet.2016.04.003 PG 16 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA DN1OJ UT WOS:000376835000011 ER EF