FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Babiker, EM Gordon, TC Chao, S Rouse, MN Wanyera, R Newcomb, M Brown-Guedira, G Pretorius, ZA Bonman, JM AF Babiker, E. M. Gordon, T. C. Chao, S. Rouse, M. N. Wanyera, R. Newcomb, M. Brown-Guedira, G. Pretorius, Z. A. Bonman, J. M. TI Genetic mapping of resistance to the Ug99 race group of Puccinia graminis f. sp tritici in a spring wheat landrace CItr 4311 SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID STEM RUST RESISTANCE; AESTIVUM L.; 1ST REPORT; VIRULENCE; TTKS; IDENTIFICATION; PATHOGEN; AFRICA; LINES; SR31 AB A gene for Ug99 resistance from wheat landrace CItr 4311 was detected on the long arm of chromosome 2B. Wheat landrace CItr 4311 has seedling resistance to stem rust caused by Puccinia graminis f. sp. tritici race TTKSK and field resistance to the Ug99 race group. Parents, F-1 seedlings, 121 doubled haploid (DH) lines, and 124 recombinant inbred lines (RILs) developed from a cross between CItr 4311 and the susceptible line LMPG-6 were evaluated for seedling resistance to race TTKSK. Goodness-of-fit tests indicated that a single dominant gene in CItr 4311 conditioned the TTKSK resistance. The 90 K wheat iSelect SNP platform was used to genotype parents and the DH population. The seedling resistance locus was mapped to the chromosome arm 2BL. Parents and the DH population were evaluated for field resistance in Kenya. One major QTL for the field resistance was consistently detected in the same region on 2BL as the seedling resistance. Using KASP assays, five linked SNP markers were used to verify the result in the 124 RIL, 35 wheat accessions, 46 DH lines from the LMPG-6/PI 165194 cross and F-1 seedlings, and susceptible bulks derived from crosses between six resistant landraces with LMPG-6. Race specificity, mapping results, and haplotype similarity with lines with Sr9h (Gabo 56, Timstein, and PI 670015), support the hypothesis that the Sr gene in CItr 4311 and the landraces is Sr9h. The KASP assays developed in this study will be useful for pyramiding the TTKSK resistance from CItr 4311 with other Sr genes effective against Ug99. C1 [Babiker, E. M.; Gordon, T. C.; Bonman, J. M.] USDA ARS, Small Grains & Potato Germplasm Res Unit, 1691 S 2700 W, Aberdeen, ID 83210 USA. [Chao, S.] USDA ARS, Cereal Crops Res Unit, 1605 Albrecht Blvd, Fargo, ND 58102 USA. [Rouse, M. N.] USDA ARS, Cereal Dis Lab, 1551 Lindig Ave, St Paul, MN 55108 USA. [Wanyera, R.] Kenya Agr & Livestock Res Org, Njoro 20107, Kenya. [Newcomb, M.] Univ Arizona, Sch Plant Sci, Maricopa, AZ 85138 USA. [Brown-Guedira, G.] USDA ARS, Plant Sci Res Unit, Raleigh, NC 27606 USA. [Pretorius, Z. A.] Univ Free State, Dept Plant Sci, ZA-9300 Bloemfontein, South Africa. RP Babiker, EM; Bonman, JM (reprint author), USDA ARS, Small Grains & Potato Germplasm Res Unit, 1691 S 2700 W, Aberdeen, ID 83210 USA. EM Ebrahiem.babiker@ars.usda.gov; Mike.Bonman@ars.usda.gov FU USDA-ARS National Plant Disease Recovery System; USDA-ARS CRIS Project [2050-21000-029-00D]; National Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2011-68002-30029] FX We are very grateful to Sam Stoxen and Jayaveeramuthu Nirmala for their technical assistance. This research was supported by the USDA-ARS National Plant Disease Recovery System, the USDA-ARS CRIS Project 2050-21000-029-00D. This work was supported in part by National Research Initiative Competitive Grant 2011-68002-30029 (Triticeae-CAP) from the USDA National Institute of Food and Agriculture. NR 28 TC 0 Z9 0 U1 17 U2 17 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD NOV PY 2016 VL 129 IS 11 BP 2161 EP 2170 DI 10.1007/s00122-016-2764-5 PG 10 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA EA4FJ UT WOS:000386565400010 PM 27544524 ER PT J AU Hiebert, CW Kassa, MT McCartney, CA You, FM Rouse, MN Fobert, P Fetch, TG AF Hiebert, Colin W. Kassa, Mulualem T. McCartney, Curt A. You, Frank M. Rouse, Matthew N. Fobert, Pierre Fetch, Tom G. TI Genetics and mapping of seedling resistance to Ug99 stem rust in winter wheat cultivar Triumph 64 and differentiation of SrTmp, SrCad, and Sr42 SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID F-SP TRITICI; INFECTION TYPES; RACE TTKS; VIRULENCE; MAP; IDENTIFICATION; MARKERS; LINES AB Resistance to Ug99 stem rust in Triumph 64 was conferred by SrTmp on chromosome arm 6DS and was mapped to the same position as SrCad and Sr42 , however, the three genes show functional differences. Stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is an important disease of wheat that can be controlled by effective stem rust resistance (Sr) genes. The emergence of virulent Pgt races in Africa, namely Ug99 and its variants, has stimulated the search for new Sr genes and genetic characterization of known sources of resistance. Triumph 64 is a winter wheat cultivar that carries gene SrTmp, which confers resistance to Ug99. The goals of this study were to genetically map SrTmp and examine its relationship with other Sr genes occupying a similar chromosome location. A doubled haploid (DH) population from the cross LMPG-6S/Triumph 64 was inoculated with Ug99 at the seedling stage. A single gene conditioning resistance to Ug99 segregated in the population. Genetic mapping with SSR markers placed SrTmp on chromosome arm 6DS in a region similar to SrCad and Sr42. SNP markers developed for SrCad were used to further map SrTmp and were also added to a genetic map of Sr42 using a DH population (LMPG-6S/Norin 40). Three SNP markers that co-segregated with SrTmp also co-segregated with SrCad and Sr42. The SNP markers showed no difference in the map locations of SrTmp, SrCad, and Sr42. Multi-race testing with DH lines from the Triumph 64 and Norin 40 populations and a recombinant inbred-line population from the cross LMPG-6S/AC Cadillac showed that SrTmp, SrCad, and Sr42 confer different spectra of resistance. Markers closely linked to SrTmp are suitable for marker-assisted breeding and germplasm development. C1 [Hiebert, Colin W.; Kassa, Mulualem T.; McCartney, Curt A.; You, Frank M.] Agr & Agri Food Canada, Morden Res & Dev Ctr, 101 Route 100, Morden, MB R6M 1Y5, Canada. [Kassa, Mulualem T.; Fobert, Pierre] CNR, 110 Gymnasium Pl, Saskatoon, SK S7N 0W9, Canada. [Rouse, Matthew N.] Univ Minnesota, USDA ARS, Cereal Dis Lab, Dept Plant Pathol, 1551 Lindig St, St Paul, MN 55108 USA. [Fetch, Tom G.] Agr & Agri Food Canada, Brandon Res & Dev Ctr, Brandon, MB R7A 5Y3, Canada. RP Hiebert, CW (reprint author), Agr & Agri Food Canada, Morden Res & Dev Ctr, 101 Route 100, Morden, MB R6M 1Y5, Canada. EM colin.hiebert@agr.gc.ca FU Agriculture and Agri-Food Canada Growing Forward; AAFC Agriflex project [2521]; AAFC A-base project [J-000006]; Canadian Wheat Alliance; CTAG and CTAG2, Genome Prairie projects - Genome Canada; Saskatchewan Ministry of Agriculture; Western Grain Research Foundation; USDA-ARS [5062-21220-021-00]; USDA-ARS National Plant Disease Recovery System; Durable Rust Resistance in Wheat project FX The authors thank Taye Zegeye, Mira Popovic, Ghassan Mardli, Leslie Bezte, Tobi Malasiuk and Elaine Martineau for excellent technical assistance. We are grateful for the financial support from Agriculture and Agri-Food Canada Growing Forward, AAFC Agriflex project 2521, AAFC A-base project J-000006, the Canadian Wheat Alliance, and as part of CTAG and CTAG2, Genome Prairie projects funded by Genome Canada, Saskatchewan Ministry of Agriculture, and Western Grain Research Foundation. M. Rouse also acknowledges the support from USDA-ARS appropriated project 5062-21220-021-00, USDA-ARS National Plant Disease Recovery System, and the Durable Rust Resistance in Wheat project. Based upon research contributions from the National Research Council of Canada, this publication was given the Contribution Number NRCC# 56224. NR 37 TC 2 Z9 2 U1 5 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD NOV PY 2016 VL 129 IS 11 BP 2171 EP 2177 DI 10.1007/s00122-016-2765-4 PG 7 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA EA4FJ UT WOS:000386565400011 PM 27506534 ER PT J AU Meinzer, FC Woodruff, DR Marias, DE Smith, DD McCulloh, KA Howard, AR Magedman, AL AF Meinzer, Frederick C. Woodruff, David R. Marias, Danielle E. Smith, Duncan D. McCulloh, Katherine A. Howard, Ava R. Magedman, Alicia L. TI Mapping "hydroscapes' along the iso- to anisohydric continuum of stomatal regulation of plant water status SO ECOLOGY LETTERS LA English DT Article DE Anisohydry; drought resistance; isohydry; leaf osmotic potential; leaf turgor; plant traits; plant water potential; stomata ID TURGOR LOSS POINT; PRESSURE-VOLUME RELATIONSHIPS; VINIFERA L. CULTIVARS; HYDRAULIC CONDUCTANCE; DROUGHT TOLERANCE; PSEUDOTSUGA-MENZIESII; EVAPORATIVE DEMAND; SEASONAL TRENDS; SUMMER DROUGHT; FOREST TREES AB The concept of iso- vs. anisohydry has been used to describe the stringency of stomatal regulation of plant water potential (). However, metrics that accurately and consistently quantify species' operating ranges along a continuum of iso- to anisohydry have been elusive. Additionally, most approaches to quantifying iso/anisohydry require labour-intensive measurements during prolonged drought. We evaluated new and previously developed metrics of stringency of stomatal regulation of during soil drying in eight woody species and determined whether easily-determined leaf pressure-volume traits could serve as proxies for their degree of iso- vs. anisohydry. Two metrics of stringency of stomatal control of , (1) a hydroscape' incorporating the landscape of over which stomata control , and (2) the slope of the daily range of as pre-dawn declined, were strongly correlated with each other and with the leaf osmotic potential at full and zero turgor derived from pressure-volume curves. C1 [Meinzer, Frederick C.; Woodruff, David R.] USDA Forest Serv, Pacific Northwest Res Stn, Corvallis, OR 97331 USA. [Marias, Danielle E.; Magedman, Alicia L.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Smith, Duncan D.; McCulloh, Katherine A.] Univ Wisconsin, Dept Bot, Madison, WI 53705 USA. [Howard, Ava R.] Western Oregon Univ, Dept Biol, Monmouth, OR 97361 USA. RP Meinzer, FC (reprint author), USDA Forest Serv, Pacific Northwest Res Stn, Corvallis, OR 97331 USA. EM Rick.Meinzer@oregonstate.edu FU NSF [IOS 11-46746] FX This work was supported by NSF grant IOS 11-46746. We are grateful to the OSU Greenhouse Operations staff members for their assistance with the maintenance of our plant material. NR 49 TC 0 Z9 0 U1 22 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-023X EI 1461-0248 J9 ECOL LETT JI Ecol. Lett. PD NOV PY 2016 VL 19 IS 11 BP 1343 EP 1352 DI 10.1111/ele.12670 PG 10 WC Ecology SC Environmental Sciences & Ecology GA DZ9VL UT WOS:000386228300005 PM 27604411 ER PT J AU Dutta, T Kim, KH Uchimiya, M Kumar, P Das, S Bhattacharya, SS Szulejko, J AF Dutta, Tanushree Kim, Ki-Hyun Uchimiya, Minori Kumar, Pawan Das, Subhasish Bhattacharya, Satya Sundar Szulejko, Jan TI The micro-environmental impact of volatile organic compound emissions from large-scale assemblies of people in a confined space SO ENVIRONMENTAL RESEARCH LA English DT Review DE VOC; Exposure; Recreation; Sports; Football ID AIR-QUALITY; INDOOR AIR; CIGARETTE-SMOKE; ANTHROPOGENIC SOURCES; COMMONWEALTH GAMES; SKIN LIPIDS; VOCS; EXPOSURE; POLLUTION; OZONE AB Large-scale assemblies of people in a confined space can exert significant impacts on the local air chemistry due to human emissions of volatile organics. Variations of air-quality in such small scale can be studied by quantifying fingerprint volatile organic compounds (VOCs) such as acetone, toluene, and isoprene produced during concerts, movie screenings, and sport events (like the Olympics and the World Cup). This review summarizes the extent of VOC accumulation resulting from a large population in a confined area or in a small open area during sporting and other recreational activities. Apart from VOCs emitted directly from human bodies (e.g., perspiration and exhaled breath), those released indirectly from other related sources (e.g., smoking, waste disposal, discharge of food-waste, and use of personal-care products) are also discussed. Although direct and indirect emissions of VOCs from human may constitute <1% of the global atmospheric VOCs budget, unique spatiotemporal variations in VOCs species within a confined space can have unforeseen impacts on the local atmosphere to lead to acute human exposure to harmful pollutants. (C) 2016 Elsevier Inc All rights reserved. C1 [Dutta, Tanushree; Kim, Ki-Hyun; Szulejko, Jan] Hanyang Univ, Dept Civil & Environm Engn, 222 Wangsimni Ro, Seoul 04763, South Korea. [Uchimiya, Minori] USDA ARS, Southern Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [Kumar, Pawan] Indian Inst Technol, Dept Chem Engn, Hauz Khas, New Delhi 11016, India. [Das, Subhasish; Bhattacharya, Satya Sundar] Tezpur Univ, Dept Environm Sci, Soil & Agrobioengn Lab, Napaam 784028, India. RP Kim, KH (reprint author), Hanyang Univ, Dept Civil & Environm Engn, 222 Wangsimni Ro, Seoul 04763, South Korea. EM kkim61@hanyang.ac.kr RI KUMAR, PAWAN /E-5522-2012 OI KUMAR, PAWAN /0000-0003-0712-8763 FU National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology (MEST) [2006-0093848]; Rural Development Administration, Republic of Korea [11010521] FX The corresponding author (KHK) acknowledges support from a National Research Foundation of Korea (NRF) grant funded by the Ministry of Education, Science and Technology (MEST) (No. 2006-0093848) and by the Cooperative Research Program for Agriculture Science & Technology Development (Project title: Study on model development to control odor from hog barn, Project No. 11010521), Rural Development Administration, Republic of Korea. NR 87 TC 0 Z9 0 U1 19 U2 19 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0013-9351 EI 1096-0953 J9 ENVIRON RES JI Environ. Res. PD NOV PY 2016 VL 151 BP 304 EP 312 DI 10.1016/j.envres.2016.08.009 PG 9 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA EA2HO UT WOS:000386413600033 PM 27522568 ER PT J AU Ma, L Yates, SR Ashworth, D AF Ma, Li Yates, Scott R. Ashworth, Daniel TI PARENT AND CONJUGATED ESTROGENS AND PROGESTAGENS IN SURFACE WATER OF THE SANTA ANA RIVER: DETERMINATION, OCCURRENCE, AND RISK ASSESSMENT SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Article DE Hormones; Occurrence; Contamination sources; Risk assessment ID TANDEM MASS-SPECTROMETRY; SOLID-PHASE EXTRACTION; ENDOCRINE DISRUPTING CHEMICALS; SEWAGE-TREATMENT WORKS; WASTE-WATER; STEROID-HORMONES; ACTIVATED-SLUDGE; TREATMENT PLANTS; DRINKING-WATER; PHARMACEUTICAL RESIDUES AB The present study investigated the occurrence of 13 parent and conjugated estrogens and progestagens in surface water of the Santa Ana River. With the exception of the synthetic hormones 17 alpha-ethynylestradiol and mestranol, other compounds were detected at least twice at 10 representative sites, with the ubiquitous estrone (E1) and 17 beta-estradiol-3-sulfate as the dominant compounds quantified (0.24-6.37 ng/L and 0.49-9.25 ng/L, respectively). Sites near dairy farms exhibited high levels of conjugates, whereas those close to a sewage treatment plant (STP) effluent outlet displayed relatively high concentrations of E1. Principle component analysis coupled with multiple linear regression revealed dairy farms and the STP as the 2 significant contamination sources, accounting for 69.9% and 31.1% of the total hormone burden, respectively. Risk assessment results suggested E1 and 17 beta-estradiol (E2) as the 2 hormones with the largest risks to aquatic organisms, and which combined, contributed >90% of the total estrogenicity. Most of the sites investigated showed that E1 and E2 posed a medium risk (0.1 < risk quotient < 1), whereas each induced a high risk (risk quotient > 1) at sites severely impacted by the STP and dairy farms. These results suggest that river health would benefit from effective treatment of waste at the STP and dairy farms prior to discharge. (C) 2016 SETAC C1 [Ma, Li; Ashworth, Daniel] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Ma, Li; Yates, Scott R.; Ashworth, Daniel] ARS, Contaminant Fate & Transport Unit, Salin Lab, USDA, Riverside, CA 92503 USA. RP Ma, L (reprint author), Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA.; Ma, L (reprint author), ARS, Contaminant Fate & Transport Unit, Salin Lab, USDA, Riverside, CA 92503 USA. EM li.ma@ars.usda.gov NR 69 TC 0 Z9 0 U1 12 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 EI 1552-8618 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD NOV PY 2016 VL 35 IS 11 BP 2657 EP 2664 DI 10.1002/etc.3447 PG 8 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA EA2XU UT WOS:000386461000003 PM 27061433 ER PT J AU Cammarano, D Rotter, RP Asseng, S Ewert, F Wallach, D Martre, P Hatfield, JL Jones, JW Rosenzweig, C Ruane, AC Boote, KJ Thorburn, PJ Kersebaum, KC Aggarwal, PK Angulo, C Basso, B Bertuzzi, P Biernath, C Brisson, N Challinor, AJ Doltra, J Gayler, S Goldberg, R Heng, L Hooker, J Hunt, LA Ingwersen, J Izaurralde, RC Muller, C Kumar, SN Nendel, C O'Leary, GJ Olesen, JE Osborne, TM Palosuo, T Priesack, E Ripoche, D Semenov, MA Shcherbak, I Steduto, P Stockle, CO Stratonovitch, P Streck, T Supit, I Tao, F Travasso, M Waha, K White, JW Wolf, J AF Cammarano, Davide Rotter, Reimund P. Asseng, Senthold Ewert, Frank Wallach, Daniel Martre, Pierre Hatfield, Jerry L. Jones, James W. Rosenzweig, Cynthia Ruane, Alex C. Boote, Kenneth J. Thorburn, Peter J. Kersebaum, Kurt Christian Aggarwal, Pramod K. Angulo, Carlos Basso, Bruno Bertuzzi, Patrick Biernath, Christian Brisson, Nadine Challinor, Andrew J. Doltra, Jordi Gayler, Sebastian Goldberg, Richie Heng, Lee Hooker, Josh Hunt, Leslie A. Ingwersen, Joachim Izaurralde, Roberto C. Mueller, Christoph Kumar, Soora Naresh Nendel, Claas O'Leary, Garry J. Olesen, Jorgen E. Osborne, Tom M. Palosuo, Taru Priesack, Eckart Ripoche, Dominique Semenov, Mikhail A. Shcherbak, Iurii Steduto, Pasquale Stockle, Claudio O. Stratonovitch, Pierre Streck, Thilo Supit, Iwan Tao, Fulu Travasso, Maria Waha, Katharina White, Jeffrey W. Wolf, Joost TI Uncertainty of wheat water use: Simulated patterns and sensitivity to temperature and CO2 SO FIELD CROPS RESEARCH LA English DT Article DE Multi-model simulation; Transpiration efficiency; Water use; Uncertainty; Sensitivity ID CLIMATE-CHANGE; USE EFFICIENCY; CROP YIELD; POTENTIAL EVAPOTRANSPIRATION; ATMOSPHERIC CO2; FOOD SECURITY; ELEVATED CO2; IMPACTS; PRODUCTIVITY; MODELS AB Projected global warming and population growth will reduce future water availability for agriculture. Thus, it is essential to increase the efficiency in using water to ensure crop productivity. Quantifying crop water use (WU; i.e. actual evapotranspiration) is a critical step towards this goal. Here, sixteen wheat simulation models were used to quantify sources of model uncertainty and to estimate the relative changes and variability between models for simulated WU, water use efficiency (WUE, WU per unit of grain dry mass produced), transpiration efficiency (T-eff, transpiration per kg of unit of grain yield dry mass produced), grain yield, crop transpiration and soil evaporation at increased temperatures and elevated atmospheric carbon dioxide concentrations ([CO2]). The greatest uncertainty in simulating water use, potential evapotranspiration, crop transpiration and soil evaporation was due to differences in how crop transpiration was modelled and accounted for 50% of the total variability among models. The simulation results for the sensitivity to temperature indicated that crop WU will decline with increasing temperature due to reduced growing seasons. The uncertainties in simulated crop WU, and in particularly due to uncertainties in simulating crop transpiration, were greater under conditions of increased temperatures and with high temperatures in combination with elevated atmospheric [CO2] concentrations. Hence the simulation of crop WU, and in particularly crop transpiration under higher temperature, needs to be improved and evaluated with field measurements before models can be used to simulate climate change impacts on future crop water demand. (C) 2016 Elsevier B.V. All rights reserved. C1 [Cammarano, Davide; Asseng, Senthold; Jones, James W.; Boote, Kenneth J.] Univ Florida, Agr & Biol Engn Dept, Gainesville, FL 32611 USA. [Rotter, Reimund P.; Palosuo, Taru; Tao, Fulu] Nat Resources Inst Finland Luke, FI-00790 Helsinki, Finland. [Ewert, Frank; Angulo, Carlos] Univ Bonn, Inst Crop Sci & Resource Conservat INRES, D-53115 Bonn, Germany. [Wallach, Daniel] INRA, Agrosyst & Dev Terr UMR1248, F-31326 Castanet Tolosan, France. [Martre, Pierre] INRA, Genet Divers & Ecophysiol Cereals GDEC UMR1095, F-63100 Clermont Ferrand, France. [Martre, Pierre] Univ Blaise Pascal, GDEC UMR1095, F-63170 Clermont Ferrand, France. [Hatfield, Jerry L.] Natl Lab Agr & Environm, Ames, IA 50011 USA. [Rosenzweig, Cynthia; Ruane, Alex C.; Goldberg, Richie] NASA, Goddard Inst Space Studies, New York, NY 10025 USA. [Thorburn, Peter J.] CSIRO, Ecosyst Sci, Dutton Pk, Qld 4102, Australia. [Kersebaum, Kurt Christian; Nendel, Claas] Leibniz Ctr Agr Landscape Res, Inst Landscape Syst Anal, D-15374 Muncheberg, Germany. [Aggarwal, Pramod K.] CIMMYT, CGIAR Res Program Climate Change Agr & Food Secur, New Delhi 110012, India. [Basso, Bruno] Michigan State Univ, Dept Geol Sci, E Lansing, MI 48824 USA. [Basso, Bruno] Michigan State Univ, Kellogg Biol Stn, E Lansing, MI 48824 USA. [Bertuzzi, Patrick; Ripoche, Dominique] INRA, AgroClim US1116, F-84914 Avignon, France. [Biernath, Christian; Priesack, Eckart] Helmholtz Zentrum Munchen, German Res Ctr Environm Hlth, Inst Soil Ecol, D-85764 Neuherberg, Germany. [Brisson, Nadine] INRA, Agron UMR0211, F-78750 Thiverval Grignon, France. [Brisson, Nadine] AgroParisTech, Agron UMR0211, F-78750 Thiverval Grignon, France. [Challinor, Andrew J.] Univ Leeds, Inst Climate & Atmospher Sci, Sch Earth & Environm, Leeds LS2 9JT, W Yorkshire, England. [Challinor, Andrew J.] CIAT, CGIAR ESSP Program Climate Change Agr & Food Secu, Cali 6713, Colombia. [Doltra, Jordi] Cantabrian Agr Res & Training Ctr CIFA, Muriedas 39600, Spain. [Gayler, Sebastian] Univ Tubingen, Water & Earth Syst Sci Competence Cluster, D-72074 Tubingen, Germany. [Heng, Lee] IAEA, A-1400 Vienna, Austria. [Hooker, Josh] Univ Reading, Sch Agr Policy & Dev, Reading RG6 6AR, Berks, England. [Hooker, Josh] Joint Res Ctr, Via Enrico Fermi 2749, I-21027 Ispra, Italy. [Hunt, Leslie A.] Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada. [Ingwersen, Joachim; Streck, Thilo] Univ Hohenheim, Inst Soil Sci & Land Evaluat, D-70599 Stuttgart, Germany. [Izaurralde, Roberto C.] Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. [Izaurralde, Roberto C.] Texas A&M Univ, Texas A&M AgriLife Res & Extens Ctr, Temple, TX 76502 USA. [Mueller, Christoph; Waha, Katharina] Potsdam Inst Climate Impact Res, D-14473 Potsdam, Germany. [Kumar, Soora Naresh] Indian Agr Res Inst, Ctr Environm Sci & Climate Resilient Agr, New Delhi 110012, India. [O'Leary, Garry J.] Dept Econ Dev Jobs Transport & Resources, Landscape & Water Sci, Horsham, Vic 3400, Australia. [Olesen, Jorgen E.] Aarhus Univ, Dept Agroecol, DK-8830 Tjele, Denmark. [Osborne, Tom M.] Univ Reading, Dept Meteorol, Natl Ctr Atmospher Sci, Reading RG6 6BB, Berks, England. [Semenov, Mikhail A.; Stratonovitch, Pierre] Rothamsted Res, Computat & Syst Biol Dept, Harpenden AL5 2JQ, Herts, England. [Steduto, Pasquale] Food & Agr Org United Nations FAO, Rome, Italy. [Shcherbak, Iurii; Stockle, Claudio O.] Washington State Univ, Biol Syst Engn, Pullman, WA 99164 USA. [Supit, Iwan] Wageningen Univ, Water Syt & Global Change Grp, NL-6700 AP Wageningen, Netherlands. [Tao, Fulu] Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China. [Travasso, Maria] INTA CIRN, Inst Climate & Water, RA-1712 Castelar, Argentina. [White, Jeffrey W.] USDA ARS, ALARC, Maricopa, AZ USA. [Wolf, Joost] Wageningen Univ, Plant Prod Syst, NL-6700 AA Wageningen 37, Netherlands. [Rotter, Reimund P.] Univ Gottingen, Dept Crop Sci, Gottingen, Germany. [Ewert, Frank] Leibniz Ctr Agr Landscape Res ZALF, D-15374 Muncheberg, Germany. [Cammarano, Davide] James Hutton Inst, Dundee DD2 5DA, Scotland. [Martre, Pierre] INRA, Montpellier SupAgro, Lab Ecophysiol Plantes Stress Environm UMR759, F-34060 Montpellier, France. [Waha, Katharina] CSIRO, Agr, 306 Carmody Rd, St Lucia, Qld 4067, Australia. RP Cammarano, D (reprint author), Univ Florida, Agr & Biol Engn Dept, Gainesville, FL 32611 USA.; Cammarano, D (reprint author), James Hutton Inst, Dundee DD2 5DA, Scotland. EM Davide.Cammarano@hutton.ac.uk RI Palosuo, Taru/B-9593-2012; Thorburn, Peter/A-6884-2011; Doltra, Jordi/C-2106-2015; Challinor, Andrew/C-4992-2008 OI Palosuo, Taru/0000-0003-4322-3450; Challinor, Andrew/0000-0002-8551-6617 FU Ministry of Science, Research and Arts of Baden-Wurttemberg [AZ Zu 33-721.3-2]; Helmholtz Center for Environmental Research, Leipzig; European FACCE MACSUR project through the Finnish Ministry of Agriculture and Forestry; INRA Environment and Agronomy Division; framework of JPI FACCE MACSUR project through the INRA Metaprogram on the Adaptation of Agriculture and Forests to Climate Change; German Federal Office for Agriculture and Food; FACCE MACSUR [2812ERA147]; COST [ES1106]; KULUNDA [01LL0905L]; FACCE MACSUR through the German Federal Ministry of Education and Research (BMBF) [031A103B]; project of Regional Approaches to Climate Change for Pacific Northwest Agriculture (REACCH-PNA) from National Institute for Food and Agriculture [2011-68002-30191] FX We thank the anonymous referees for the valuable comments and suggestions that helped improve the manuscript. S.G. was supported by a grant from the Ministry of Science, Research and Arts of Baden-Wurttemberg (AZ Zu 33-721.3-2) and the Helmholtz Center for Environmental Research, Leipzig (UFZ); R.P.R., T.P. and F.T. were supported by funds from the European FACCE MACSUR project through the Finnish Ministry of Agriculture and Forestry; P.M., P.B., N.B. and D.R. were supported by INRA Environment and Agronomy Division and by the funding within the framework of JPI FACCE MACSUR project through the INRA Metaprogram on the Adaptation of Agriculture and Forests to Climate Change; K.C.K. and C.N. received support from the German Federal Office for Agriculture and Food with FACCE MACSUR (2812ERA147) and from COST ES1106; C.M. acknowledges financial support from the KULUNDA project (01LL0905L) and the FACCE MACSUR project (031A103B) funded through the German Federal Ministry of Education and Research (BMBF); C.O.S. was supported by the project of Regional Approaches to Climate Change for Pacific Northwest Agriculture (REACCH-PNA) funded through award #2011-68002-30191 from the National Institute for Food and Agriculture. This work has been carried out under the framework of the Agricultural Model Inter comparison and Improvment Project (AgMIP). NR 67 TC 2 Z9 2 U1 48 U2 48 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4290 EI 1872-6852 J9 FIELD CROP RES JI Field Crop. Res. PD NOV PY 2016 VL 198 BP 80 EP 92 DI 10.1016/j.fcr.2016.08.015 PG 13 WC Agronomy SC Agriculture GA EA2FX UT WOS:000386409300009 ER PT J AU Kalsbeek, A Veenstra, J Westra, J Disselkoen, C Smith, C Tintle, N AF Kalsbeek, Anya Veenstra, Jenna Westra, Jason Disselkoen, Craig Smith, Caren Tintle, Nathan TI Genome-Wide Interaction Study of Red-Blood Cell Fatty Acids on Inflammatory Biomarkers in the Framingham Heart Study SO GENETIC EPIDEMIOLOGY LA English DT Meeting Abstract CT Annual Meeting of the International-Genetic-Epidemiology-Society CY OCT 24-26, 2016 CL Toronto, CANADA SP Int Genet Epidemiol Soc C1 [Kalsbeek, Anya; Veenstra, Jenna; Westra, Jason; Disselkoen, Craig; Tintle, Nathan] Dordt Coll, Dept Math Stat & Comp Sci, Sioux Ctr, IA USA. [Smith, Caren] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0741-0395 EI 1098-2272 J9 GENET EPIDEMIOL JI Genet. Epidemiol. PD NOV PY 2016 VL 40 IS 7 MA 97 BP 644 EP 644 PG 1 WC Genetics & Heredity; Mathematical & Computational Biology SC Genetics & Heredity; Mathematical & Computational Biology GA DZ7HF UT WOS:000386034800103 ER PT J AU Grant, MR Tymon, LS Helms, GL Thomashow, LS Keller, CK Harsh, JB AF Grant, M. R. Tymon, L. S. Helms, G. L. Thomashow, L. S. Keller, C. Kent Harsh, J. B. TI Biofilm adaptation to iron availability in the presence of biotite and consequences for chemical weathering SO GEOBIOLOGY LA English DT Article ID PSEUDOMONAS-AERUGINOSA; MICROBIAL POLYSACCHARIDES; BACTERIAL COMMUNITIES; FELDSPAR DISSOLUTION; NMR-SPECTROSCOPY; INHIBITION; CORROSION; MINERALS; SURFACES; METALS AB Bacteria in nature often live within biofilms, exopolymeric matrices that provide a favorable environment that can differ markedly from their surroundings. Biofilms have been found growing on mineral surfaces and are expected to play a role in weathering those surfaces, but a clear understanding of how environmental factors, such as trace-nutrient limitation, influence this role is lacking. Here, we examine biofilm development by Pseudomonas putida in media either deficient or sufficient in Fe during growth on biotite, an Fe rich mineral, or on glass. We hypothesized that the bacteria would respond to Fe deficiency by enhancing biotite dissolution and by the formation of binding sites to inhibit Fe leaching from the system. Glass coupons acted as a no-Fe control to investigate whether biofilm response depended on the presence of Fe in the supporting solid. Biofilms grown on biotite, as compared to glass, had significantly greater biofilm biomass, specific numbers of viable cells (SNVC), and biofilm cation concentrations of K, Mg, and Fe, and these differences were greater when Fe was deficient in the medium. Scanning electron microscopy (SEM) confirmed that biofilm growth altered the biotite surface, smoothing the rough, jagged edges of channels scratched by hand on the biotite, and dissolving away small, easy-to-access particles scattered across the planar surface. High-resolution magic angle spinning proton nuclear magnetic resonance (HRMAS H-1 NMR) spectroscopy showed that, in the Fe-deficient medium, the relative amount of polysaccharide nearly doubled relative to that in biofilms grown in the medium amended with Fe. The results imply that the bacteria responded to the Fe deficiency by obtaining Fe from biotite and used the biofilm matrix to enhance weathering and as a sink for released cation nutrients. These results demonstrate one mechanism by which biofilms may help soil microbes overcome nutrient deficiencies in oligotrophic systems. C1 [Grant, M. R.; Harsh, J. B.] Washington State Univ, Dept Crops & Soil Sci, Pullman, WA 99164 USA. [Tymon, L. S.] Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83843 USA. [Helms, G. L.] Washington State Univ, Nucl Magnet Resonance Spect Ctr, Pullman, WA 99164 USA. [Thomashow, L. S.] Washington State Univ, Root Dis & Biol Control Res Unit, USDA ARS, Pullman, WA 99164 USA. [Keller, C. Kent] Washington State Univ, Sch Environm, Pullman, WA 99164 USA. RP Grant, MR (reprint author), Washington State Univ, Dept Crops & Soil Sci, Pullman, WA 99164 USA. EM michael.grant@wsu.edu RI Harsh, James/C-7455-2014 OI Harsh, James/0000-0002-0177-3342 FU NSF/EAR grant [0952399] FX Thanks are due to Jeff Boyle and Dr. Scott Boroughs both at Washington State University for their analytical assistance and to Dr. Tarah Sullivan for her careful critique of this manuscript. This research was financially supported by NSF/EAR grant No. 0952399 to C. Kent Keller and collaborators. Thanks are also due to the staff at the Washington State University Franceschi Microscopy and Imaging Center for their help using the SEM. I would also like to thank my soil chemistry and physics laboratory group at Washington State University who helped guide me in my journey to make this project a reality. NR 80 TC 0 Z9 0 U1 14 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1472-4677 EI 1472-4669 J9 GEOBIOLOGY JI Geobiology PD NOV PY 2016 VL 14 IS 6 BP 588 EP 598 DI 10.1111/gbi.12187 PG 11 WC Biology; Environmental Sciences; Geosciences, Multidisciplinary SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Geology GA DZ7EF UT WOS:000386026800005 PM 27384343 ER PT J AU Jenerette, GD Clarke, LW Avolio, ML Pataki, DE Gillespie, TW Pincetl, S Nowak, DJ Hutyra, LR McHale, M McFadden, JP Alonzo, M AF Jenerette, G. Darrel Clarke, Lorraine W. Avolio, Meghan L. Pataki, Diane E. Gillespie, Thomas W. Pincetl, Stephanie Nowak, Dave J. Hutyra, Lucy R. McHale, Melissa McFadden, Joseph P. Alonzo, Michael TI Climate tolerances and trait choices shape continental patterns of urban tree biodiversity SO GLOBAL ECOLOGY AND BIOGEOGRAPHY LA English DT Article DE Biodiversity; ecosystem service; environmental filter; macroecology; tree; plant trait; urban ID SPECIES RICHNESS; PLANT DIVERSITY; LOS-ANGELES; CITIES; URBANIZATION; FLORAS; FORESTS; GARDENS; HOMOGENIZATION; COMMUNITIES AB AimWe propose and test a climate tolerance and trait choice hypothesis of urban macroecological variation in which strong filtering associated with low winter temperatures restricts urban biodiversity while weak filtering associated with warmer temperatures and irrigation allows dispersal of species from a global source pool, thereby increasing urban biodiversity. LocationTwenty cities across the USA and Canada. MethodsWe examined variation in tree community taxonomic diversity, origins and production of an aesthetic ecosystem service trait in a cross-section of urban field surveys. We correlated urban tree community composition indicators with a key climate restriction, namely mean minimum winter temperature, and evaluated alternative possible drivers: precipitation, summer maximum temperature, population size and the percentage of adults with a college education. ResultsSpecies accumulation curves differed substantially among cities, with observed richness varying from 22 to 122 species. Similarities in tree communities decreased exponentially with increases in climatic differences. Ordination of tree communities showed strong separation among cities with component axes correlated with minimum winter temperature and annual precipitation. Variation among urban tree communities in richness, origins and the provisioning of an aesthetic ecosystem service were all correlated with minimum winter temperature. Main conclusionsThe urban climate tolerance and trait choice hypothesis provides a coherent mechanism to explain the large variation among urban tree communities resulting from an interacting environment, species and human decisions. Reconciling the feedbacks between human decision making and biophysical limitations provides a foundation for an urban ecological theory that can better understand and predict the dynamics of other linked biotic communities, associated ecosystem dynamics and resulting services provided to urban residents. C1 [Jenerette, G. Darrel; Clarke, Lorraine W.] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92512 USA. [Avolio, Meghan L.; Pataki, Diane E.] Univ Utah, Dept Biol, Salt Lake City, UT 84112 USA. [Gillespie, Thomas W.; Pincetl, Stephanie] Univ Calif Los Angeles, Dept Geog, Los Angeles, CA 90095 USA. [Nowak, Dave J.] US Forest Serv, Northern Res Stn, Syracuse, NY 13210 USA. [Hutyra, Lucy R.] Boston Univ, Dept Earth & Environm, Boston, MA 02215 USA. [McHale, Melissa] North Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [McFadden, Joseph P.; Alonzo, Michael] Univ Calif Santa Barbara, Dept Geog, Santa Barbara, CA 93106 USA. [Clarke, Lorraine W.] Univ Dist Columbia, Washington, DC USA. [Avolio, Meghan L.] Natl Socioenvironm Synth Ctr, Annapolis, MD USA. RP Jenerette, GD (reprint author), Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. EM darrel.jenerette@ucr.edu FU National Science Foundation [DEB-0919006, DEB-0919381, DEB-0918905, GEO-0814692, BCS-1026865, DEB-1149471, BCS-0948914]; USFS; National Aeronautic and Space Administration [NNG04GN80G]; USDA FX This work was supported by the National Science Foundation through DEB-0919006; DEB-0919381; DEB-0918905; GEO-0814692; BCS-1026865; DEB-1149471; BCS-0948914; USFS, National Aeronautic and Space Administration through NNG04GN80G, and USDA. We appreciate the help of the many research teams who helped collect the tree sample data. We thank Jeff Diez, Karl Evans, Sharon Hall and Julie Ripplinger for insightful discussions and comments on our manuscript and Jun Yang for readily sharing data. NR 60 TC 0 Z9 0 U1 12 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1466-822X EI 1466-8238 J9 GLOBAL ECOL BIOGEOGR JI Glob. Ecol. Biogeogr. PD NOV PY 2016 VL 25 IS 11 BP 1367 EP 1376 DI 10.1111/geb.12499 PG 10 WC Ecology; Geography, Physical SC Environmental Sciences & Ecology; Physical Geography GA EA1WJ UT WOS:000386382500009 ER PT J AU O'Donnell, K Sutton, DA Wiederhold, N Robert, VARG Crous, PW Geiser, DM AF O'Donnell, Kerry Sutton, Deanna A. Wiederhold, Nathan Robert, Vincent A. R. G. Crous, Pedro W. Geiser, David M. TI Veterinary Fusarioses within the United States SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID SOLANI SPECIES COMPLEX; MOLECULAR PHYLOGENETIC DIVERSITY; DNA-SEQUENCE DATABASE; CONTACT-LENS; INFECTIONS; MYCOSIS; MEMBERS; ORIGIN; FUNGUS; COMMON AB Multilocus DNA sequence data were used to assess the genetic diversity and evolutionary relationships of 67 Fusarium strains from veterinary sources, most of which were from the United States. Molecular phylogenetic analyses revealed that the strains comprised 23 phylogenetically distinct species, all but two of which were previously known to infect humans, distributed among eight species complexes. The majority of the veterinary isolates (47/67 = 70.1%) were nested within the Fusarium solani species complex (FSSC), and these included 8 phylospecies and 33 unique 3-locus sequence types (STs). Three of the FSSC species (Fusarium falciforme, Fusarium keratoplasticum, and Fusarium sp. FSSC 12) accounted for four-fifths of the veterinary strains (38/47) and STs (27/33) within this clade. Most of the F. falciforme strains (12/15) were recovered from equine keratitis infections; however, strains of F. keratoplasticum and Fusarium sp. FSSC 12 were mostly (25/27) isolated from marine vertebrates and invertebrates. Our sampling suggests that the Fusarium incarnatum-equiseti species complex (FIESC), with eight mycoses-associated species, may represent the second most important clade of veterinary relevance within Fusarium. Six of the multilocus STs within the FSSC (3+4-eee, 1-b, 12-a, 12-b, 12-f, and 12-h) and one each within the FIESC (1-a) and the Fusarium oxysporum species complex (ST-33) were widespread geographically, including three STs with transoceanic disjunctions. In conclusion, fusaria associated with veterinary mycoses are phylogenetically diverse and typically can only be identified to the species level using DNA sequence data from portions of one or more informative genes. C1 [O'Donnell, Kerry] ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, USDA, Peoria, IL USA. [Sutton, Deanna A.; Wiederhold, Nathan] Univ Texas Hlth Sci Ctr San Antonio, Dept Pathol, San Antonio, TX 78229 USA. [Robert, Vincent A. R. G.; Crous, Pedro W.] CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands. [Geiser, David M.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. RP O'Donnell, K (reprint author), ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, USDA, Peoria, IL USA. EM kerry.odonnell@ars.usda.gov NR 41 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 EI 1098-660X J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD NOV PY 2016 VL 54 IS 11 BP 2813 EP 2819 DI 10.1128/JCM.01607-16 PG 7 WC Microbiology SC Microbiology GA DZ8ID UT WOS:000386113200027 PM 27605713 ER PT J AU Taylor, MK Lankau, RA Wurzburger, N AF Taylor, Melanie K. Lankau, Richard A. Wurzburger, Nina TI Mycorrhizal associations of trees have different indirect effects on organic matter decomposition SO JOURNAL OF ECOLOGY LA English DT Article DE arbuscular mycorrhizas; biogeochemistry; carbon; decomposition; ectomycorrhizas; nitrogen; plant-soil relationship; soil organic matter ID LITTER DECOMPOSITION; CARBON SEQUESTRATION; ECTOMYCORRHIZAL COLONIZATION; ACQUISITION STRATEGIES; ROOT DECOMPOSITION; TEMPERATE FOREST; BOREAL FOREST; SOIL; FUNGI; NITROGEN AB Organic matter decomposition is the main process by which carbon (C) is lost from terrestrial ecosystems, and mycorrhizal associations of plants (i.e. arbuscular mycorrhizas (AM) and ectomycorrhizas (ECM)) may have different indirect effects on this loss pathway. AM and ECM plants differ in the soil decomposers they promote and the quality of litter they produce, which may result in different patterns of organic matter decomposition, and hence, soil C loss. To determine how mycorrhizal associations indirectly affect decomposer activity, we collected soils and litters from four AM and four ECM tree species from a mixed-deciduous temperate forest for a field and laboratory study. We first characterized insitu patterns in soil chemistry and soil microbial biomass among these eight tree species. We then conducted a microcosm experiment with mineral soils, leaf litter and fine roots originating from these tree species, where we reciprocally crossed litters and soils, and quantified the rate of heterotrophic respiration over a 140-day laboratory incubation. In natural forest conditions, AM tree soils contained lower total C and microbial biomass C:N relative to ECM tree soils. In our microcosm experiment, AM soils supported greater heterotrophic respiration than did ECM soils. The addition of AM litter stimulated respiration more than did ECM litter, owing to the lower C:N of AM litter. Matching the mycorrhizal identity of litter and soil resulted in a difference in total respiration, such that combinations of AM litters with AM soils lost more C than did combinations of ECM litters with ECM soils.Synthesis. Our findings demonstrate that AM and ECM trees have differing indirect effects on soil decomposer activity through the decomposers they cultivate and/or the quality of organic matter they produce. Mycorrhizal differences in litter quality accentuate these effects on soil C loss and may explain patterns in soil C dynamics in terrestrial ecosystems. C1 [Taylor, Melanie K.; Wurzburger, Nina] Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. [Lankau, Richard A.] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA. [Taylor, Melanie K.] USDA Forest Serv, So Res Stn, Athens, GA 30602 USA. RP Wurzburger, N (reprint author), Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. EM ninawurz@uga.edu NR 51 TC 2 Z9 2 U1 57 U2 57 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-0477 EI 1365-2745 J9 J ECOL JI J. Ecol. PD NOV PY 2016 VL 104 IS 6 BP 1576 EP 1584 DI 10.1111/1365-2745.12629 PG 9 WC Plant Sciences; Ecology SC Plant Sciences; Environmental Sciences & Ecology GA DZ5PY UT WOS:000385915200007 ER PT J AU Waller, LP Callaway, RM Klironomos, JN Ortega, YK Maron, JL AF Waller, Lauren P. Callaway, Ragan M. Klironomos, John N. Ortega, Yvette K. Maron, John L. TI Reduced mycorrhizal responsiveness leads to increased competitive tolerance in an invasive exotic plant SO JOURNAL OF ECOLOGY LA English DT Article DE Arbuscular mycorrhizal fungi; Centaurea solstitialis; competition; invasion; mycorrhizal responsiveness; Stipa pulchra ID VESICULAR-ARBUSCULAR MYCORRHIZAE; TALLGRASS PRAIRIE; GROWTH-RESPONSES; SOIL DISTURBANCE; CARBON TRANSFER; C-14 TRANSFER; FUNGI; EVOLUTION; DIVERSITY; PHOSPHORUS AB Arbuscular mycorrhizal (AM) fungi can exert a powerful influence on the outcome of plant-plant competition. Since some exotic plants interact differently with soil biota such as AM fungi in their new range, range-based shifts in AM responsiveness could shift competitive interactions between exotic and resident plants, although this remains poorly studied. We explored whether genotypes of the annual exotic Centaurea solstitialis (yellow starthistle), collected from populations across the native and non-native ranges, differed in responsiveness to AM fungi in the introduced range and whether range-based differences in mycorrhizal responsiveness affected how strongly C. solstitialis tolerated competition with the North American native bunchgrass, Stipa pulchra. Grown alone, C. solstitialis from both ranges derived only weak benefits from AM fungi. However, association with AM fungi was costly to plants when grown in competition with S. pulchra. The magnitude of the suppressive effect of AM fungi was greater for genotypes from native versus introduced populations.Synthesis. Many exotic invasive species are known to associate weakly with AM fungi, which may be beneficial in disturbed habitats where competition for resources is low. Our results indicate that reduced mycorrhizal associations may also benefit invaders in a competitive environment. Centaurea solstitialis were more strongly suppressed by established S. pulchra plants in the presence versus absence of AM fungi, but exotic genotypes were less suppressed than native genotypes. This suggests that AM fungi may contribute to invasion resistance in established native communities, but range-based shifts in the way exotic genotypes respond to AM fungal partners may counter such biotic resistance. C1 [Waller, Lauren P.; Callaway, Ragan M.; Maron, John L.] Univ Montana, Div Biol Sci, 32 Campus Dr,HS 104, Missoula, MT USA. [Callaway, Ragan M.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Callaway, Ragan M.] Univ Montana, Inst Ecosyst, Missoula, MT 59812 USA. [Klironomos, John N.] Univ British Columbia Okanagan, Dept Biol, Kelowna, BC V1V 1V7, Canada. [Ortega, Yvette K.] US Dept Agr Forest Serv, Rocky Mt Res Stn, Missoula, MT 59081 USA. RP Waller, LP (reprint author), Lincoln Univ, Bioprotect Res Ctr, POB 84, Lincoln 7647, New Zealand. EM lauren.waller@lincoln.ac.nz FU NSF [DEB 0614406]; NSF EPSCoR Track-1 [EPS-1101342] FX This work was funded by an NSF grant DEB 0614406 to JLM and RMC. RMC thanks the NSF EPSCoR Track-1 EPS-1101342 (INSTEP 3) for support. We thank Jose Hierro and Dani Montesinos for help collecting seeds and Giles Thelen and Jill Gebhart Wood for help collecting soil. Tyler Albrethsen, Whitney Warren and David Hooper provided support in the glasshouse. We are very grateful to Drs Ylva Lekberg, Anna Sala and three previous anonymous referees whose helpful comments improved the manuscript. The authors have no conflicts of interest to declare. NR 68 TC 0 Z9 0 U1 39 U2 39 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-0477 EI 1365-2745 J9 J ECOL JI J. Ecol. PD NOV PY 2016 VL 104 IS 6 BP 1599 EP 1607 DI 10.1111/1365-2745.12641 PG 9 WC Plant Sciences; Ecology SC Plant Sciences; Environmental Sciences & Ecology GA DZ5PY UT WOS:000385915200009 ER PT J AU Alshaya, A Hunt, J Rowlands, R AF Alshaya, A. Hunt, J. Rowlands, R. TI Stresses and Strains in Thick Perforated Orthotropic Plates SO JOURNAL OF ENGINEERING MECHANICS LA English DT Article DE Holes; Thick plates; Three-dimensional stresses; Three-dimensional strains; Constraint factors; Orthotropy; Sitka Spruce; Carbon; epoxy composite ID ELASTIC PLATE; HOLE; FIELD AB Stress and strain concentrations and in-plane and out-of-plane stress constraint factors associated with a circular hole in thick, loaded orthotropic composite plates are determined by three-dimensional finite element method. The plate has essentially infinite in-plane geometry but finite thickness. Results for Sitka spruce wood are emphasized, although some for carbon-epoxy composites are included. While some results are similar to those for isotropy, there are significant consequences due to material orthotropy. Maximum stress and strain concentration factors occur at midplane for thin plates but closer to the external traction-free surfaces for thick plates. These factors decrease as the plate surface is approached and reach lower values unrepresentative of the maximum values. Differences between the midplane and/or maximum and surface stress or strain concentration factors in Sitka spruce, range from 8% if the wood grain is parallel to the vertically applied load to 15% when the grain is perpendicular to the load. These values exceed those typically reported for isotropic materials. Stress and strain concentration factors tend to differ in magnitude from each other. The combination of high local stresses and directional strength dependency of orthotropic materials can be particularly important. That maximum stress and/or strain concentrations in thick plates occur on other than the external plate surfaces where they are most readily measured is technically significant. The E11/E22 ratio in Sitka Spruce exceeds that in the carbon composite by 60%. However, when loading parallel to the strong/stiff directions, the plane-stress tensile stress concentration factors of the two materials are comparable to each other. (C) 2016 American Society of Civil Engineers. C1 [Alshaya, A.; Rowlands, R.] Univ Wisconsin, Madison, WI 53706 USA. [Hunt, J.] USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Rowlands, R (reprint author), Univ Wisconsin, Madison, WI 53706 USA. EM alshaya@wisc.edu; jfhunt@fs.fed.us; rowlands@engr.wisc.edu FU Kuwait University graduate student scholarship FX The authors thank Andrew Mikkelson, UW-Madison, for technical discussions and help in the finite element modeling. Abdullah Alshaya was supported by a Kuwait University graduate student scholarship. NR 20 TC 0 Z9 0 U1 6 U2 6 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9399 EI 1943-7889 J9 J ENG MECH JI J. Eng. Mech. PD NOV PY 2016 VL 142 IS 11 AR 04016082 DI 10.1061/(ASCE)EM.1943-7889.0001138 PG 10 WC Engineering, Mechanical SC Engineering GA EA1OU UT WOS:000386362500001 ER PT J AU Goldstein, A Foti, R Montalto, F AF Goldstein, Alisha Foti, Romano Montalto, Franco TI Effect of Spatial Resolution in Modeling Stormwater Runoff for an Urban Block SO JOURNAL OF HYDROLOGIC ENGINEERING LA English DT Article ID MULTIOBJECTIVE CALIBRATION; AGGREGATION AB This study assesses the role of spatial resolution in hydrologic and hydraulic models by means of two models developed at different resolutions for an urban block using the U.S. Environmental Protection Agency's stormwater management model (SWMM). The first is a high-resolution model, in which distinct elements are represented as individual hydrologic response units (HRU). The second is a low-resolution model, in which the entire block is represented as a single HRU. Flow observations during five storms were used to calibrate the models, while a separate set of six storms was used to assess their performance. The high-resolution model was found to systematically underestimate peak flows, whereas the performance of the low-resolution model was found to be dependent on the type of storm. Overall, the two models displayed similar accuracy according to most metrics, thus justifying modeling at high resolution only in those cases where a detailed representation is necessary. C1 [Goldstein, Alisha] DC Dept Energy & Environm, 1200 First St,NE 5th Floor, Washington, DC 20052 USA. [Goldstein, Alisha; Foti, Romano; Montalto, Franco] Drexel Univ, Dept Civil Architectural & Environm Engn, Curtis Hall Room 251,3141 Chestnut St, Philadelphia, PA 19104 USA. [Montalto, Franco] US Forest Serv, New York City Urban Field Stn, Bayside, NY 11359 USA. RP Montalto, F (reprint author), Drexel Univ, Dept Civil Architectural & Environm Engn, Curtis Hall Room 251,3141 Chestnut St, Philadelphia, PA 19104 USA.; Montalto, F (reprint author), US Forest Serv, New York City Urban Field Stn, Bayside, NY 11359 USA. EM aeg2107@gmail.com; rf376@drexel.edu; Fam26@drexel.edu FU New York State Office of the Attorney General FX The authors would like to acknowledge Omar Khader and Kim DiGiovanni for conducting the initial site visits, collecting all the observed data and devising a preliminary model. Much gratitude is given to Hugh Gordon who assisted with the program code. The authors also would like to extend special thanks to New York State Office of the Attorney General for funding the research and the following co-collaborators: Youth Ministries for Peace and Justice, NYC Department of Environmental Protection, NYC Department of Parks and Recreation and HydroQual, Inc. NR 12 TC 0 Z9 0 U1 14 U2 14 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 1084-0699 EI 1943-5584 J9 J HYDROL ENG JI J. Hydrol. Eng. PD NOV PY 2016 VL 21 IS 11 AR 06016009 DI 10.1061/(ASCE)HE.1943-5584.0001377 PG 5 WC Engineering, Civil; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA EA1QR UT WOS:000386367400002 ER PT J AU Bautista, E Warrick, AW Schlegel, JL Thorp, KR Hunsaker, DJ AF Bautista, E. Warrick, A. W. Schlegel, J. L. Thorp, K. R. Hunsaker, D. J. TI Approximate Furrow Infiltration Model for Time-Variable Ponding Depth SO JOURNAL OF IRRIGATION AND DRAINAGE ENGINEERING LA English DT Article ID 3-DIMENSIONAL ANALYSIS; IRRIGATION ADVANCE; DISC INFILTROMETER; SOLUTE TRANSPORT; WATER; FLOW; SOILS; SIMULATION; EQUATION; SURFACE AB A methodology is proposed for estimating furrow infiltration under time-variable ponding depth. The methodology approximates the solution to the two-dimensional Richards equation, and is a modification of a procedure that was originally proposed for computing infiltration under constant ponding depth. Two computational approaches were developed and tested using several combinations of soil hydraulic properties, furrow geometry, and flow depth variations. Both methods yielded solutions of reasonable and similar accuracy relative to numerical solutions of the two-dimensional Richards equation. The analysis also showed that the accuracy of the approximate model varies mostly as a function of soil hydraulic properties. The accuracy of the approximate solution can be improved with calibration. Two calibration methods were examined, one assuming that the calibration parameter varies with depth, and the other assuming a constant value. The analysis showed that latter approach, in combination with one of the proposed computational methods, reproduced the Richards equation solution more accurately. This means that a unique calibration parameter can be developed for the particular soil and geometric configuration conditions, and applied to different patterns of ponding depth variation. (C) 2016 American Society of Civil Engineers. C1 [Bautista, E.; Schlegel, J. L.; Thorp, K. R.; Hunsaker, D. J.] ARS, US Arid Land Agr Res Ctr, USDA, 21881N Cardon Ln, Maricopa, AZ 85138 USA. [Warrick, A. W.] Univ Arizona, Dept Soil Water & Environm Sci, Tucson, AZ 85721 USA. RP Bautista, E (reprint author), ARS, US Arid Land Agr Res Ctr, USDA, 21881N Cardon Ln, Maricopa, AZ 85138 USA. EM Eduardo.Bautista@ars.usda.gov; aww@cals.arizona.edu; James.Schlegel@ars.usda.gov; Kelly.Thorp@ars.usda.gov; Doug.Hunsaker@ars.usda.gov NR 32 TC 0 Z9 0 U1 1 U2 1 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9437 EI 1943-4774 J9 J IRRIG DRAIN ENG JI J. Irrig. Drainage Eng-ASCE PD NOV PY 2016 VL 142 IS 11 AR 04016045 DI 10.1061/(ASCE)IR.1943-4774.0001057 PG 11 WC Agricultural Engineering; Engineering, Civil; Water Resources SC Agriculture; Engineering; Water Resources GA EA1MZ UT WOS:000386357700003 ER PT J AU Henry, CG Hirsh, SL Anders, MM Vories, ED Reba, ML Watkins, KB Hardke, JT AF Henry, C. G. Hirsh, S. L. Anders, M. M. Vories, E. D. Reba, M. L. Watkins, K. B. Hardke, J. T. TI Annual Irrigation Water Use for Arkansas Rice Production SO JOURNAL OF IRRIGATION AND DRAINAGE ENGINEERING LA English DT Article DE Contour-levee rice irrigation; Multiple inlet rice irrigation; Straight-levee rice irrigation; Zero-grade rice AB This study investigated rice irrigation water use in the University of Arkansas Rice Research Verification Program between the years of 2003 and 2012. Annual irrigation water use averaged 763mm (30.0in.) over 10years. A significant (40%) water savings was found for rice grown using a zero-grade irrigation system [486mm (19.1in.)] compared to contour-levee or straight-levee systems. No differences in irrigation water use were found between contour-levee systems [814mm (32.1in.)] and straight-levee systems [822mm (32.4in.)]. Surface water irrigators used significantly less irrigation water [624mm (24.6in.)] than groundwater well fields [786mm (30.7in.)] and cost US$77.51 less per hectare (US$31.37 less per acre) to operate. Diesel pumps cost significantly more [$88.38/acre ($218.29/ha)] to operate than electric pumps [$58.69/acre (145.96/ha)] (P=0.02), although fields that used diesel as an energy source averaged 152mm (6.0in.) less irrigation water than electric pumps. Arkansas producers implementing the multiple-inlet water delivery practice on contour-levee or straight-levee systems, irrespective of soil type, did not realize a water savings but did realize improved yields. These results are in contrast to those in earlier studies and suggest that an educational effort may be necessary if the field research-proven multiple-inlet water savings are to be realized. C1 [Henry, C. G.] Univ Arkansas, Rice Res & Extens Ctr, Biol & Agr Engn, 2900 Hwy 130 East, Stuttgart, AR 72160 USA. [Hirsh, S. L.] Univ Maryland, Dept Environm Sci & Technol, 1443 Anim Sci Bldg, College Pk, MD 20742 USA. [Anders, M. M.] Univ Arkansas, Rice Res & Extens Ctr, Crop Soil & Environm Sci, 2900 Hwy 130 East, Stuttgart, AR 72160 USA. [Vories, E. D.] Univ Missouri, Delta Ctr, USDA, ARS, POB 160,147 State Highway T, Portageville, MO 63873 USA. [Reba, M. L.] ARS, USDA, Delta Water Management Res Unit, 504 Univ Loop East, Jonesboro, AR 72401 USA. [Watkins, K. B.] Univ Arkansas, Dept Agr Econ, Rice Res & Extens Ctr, 2900 Hwy 130 East, Stuttgart, AR 72160 USA. [Hardke, J. T.] Univ Arkansas, Rice Res & Extens Ctr, Soil & Environm Sci, 2900 Hwy 130 East, Stuttgart, AR 72160 USA. RP Henry, CG (reprint author), Univ Arkansas, Rice Res & Extens Ctr, Biol & Agr Engn, 2900 Hwy 130 East, Stuttgart, AR 72160 USA. EM cghenry@uark.edu; mmanders@uark.edu; Earl.Vories@ars.usda.gov; michele.reba@ars.usda.gov; kbwatkins@uark.edu; jhardke@uaex.edu FU Arkansas Rice Research and Promotion Board FX The authors would like to thank Phil Tacker and all the past verification coordinators and faculty project leaders throughout the history of the RRVP program for their efforts in collecting these data. These data would not have been available without the long-term financial support of the Arkansas Rice Research and Promotion Board. NR 7 TC 0 Z9 0 U1 6 U2 6 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9437 EI 1943-4774 J9 J IRRIG DRAIN ENG JI J. Irrig. Drainage Eng-ASCE PD NOV PY 2016 VL 142 IS 11 AR 05016006 DI 10.1061/(ASCE)IR.1943-4774.0001068 PG 5 WC Agricultural Engineering; Engineering, Civil; Water Resources SC Agriculture; Engineering; Water Resources GA EA1MZ UT WOS:000386357700005 ER PT J AU Johnson, ET Dowd, PF AF Johnson, Eric T. Dowd, Patrick F. TI A quantitative method for determining relative colonization rates of maize callus by Fusarium graminearum for resistance gene evaluations SO JOURNAL OF MICROBIOLOGICAL METHODS LA English DT Article DE Maize; Mycotoxin; Callus; Pathogen resistance; Peroxidase ID INSECT RESISTANCE; TISSUE-CULTURE; EXPRESSION; ROT; PLANTS AB A quantitative PCR method was developed for detecting Fusarium graminearum growing in maize callus. Fungal DNA was detected 12 h after inoculation (detection limit, 0.2 pg) and was correlated with visual ratings. The method effectively quantified fungal growth in callus overexpressing a peroxidase gene conferring fungal resistance. Published by Elsevier B.V. C1 [Johnson, Eric T.; Dowd, Patrick F.] USDA ARS, Crop Bioprotect Res, 1815 N Univ St, Peoria, IL 61604 USA. RP Johnson, ET (reprint author), USDA ARS, Crop Bioprotect Res, 1815 N Univ St, Peoria, IL 61604 USA. EM eric.johnson2@ars.usda.gov FU ARS project [5010-42000-047-00] FX The mention of trade names or commercial products in this paper is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture over other firms or similar products not mentioned. USDA is an equal opportunity provider and employer. This research was supported by funds from ARS project 5010-42000-047-00. NR 18 TC 0 Z9 0 U1 6 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-7012 EI 1872-8359 J9 J MICROBIOL METH JI J. Microbiol. Methods PD NOV PY 2016 VL 130 BP 73 EP 75 DI 10.1016/j.mimet.2016.08.026 PG 3 WC Biochemical Research Methods; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA EA2EZ UT WOS:000386406900011 PM 27577788 ER PT J AU Kiszonas, AM Fuerst, EP Talbert, L Sherman, J Morris, CF AF Kiszonas, Alecia M. Fuerst, E. Patrick Talbert, Luther Sherman, Jamie Morris, Craig F. TI Effect of wheat (Triticum aestivum L.) seed color and hardness genes on the consumption preference of the house mouse (Mus musculus L.) SO MAMMALIA LA English DT Article DE feeding preference; grain hardness; house mouse; seed color; wheat grain ID FOOD PREFERENCES; SPRING WHEAT; GRAIN HARDNESS; MICE; RED; KERNEL; PUROINDOLINES; REGISTRATION; VARIETIES; ENDOSPERM AB Wheat (Triticum aestivum L.) seeds are a primary food of the house mouse (Mus musculus L.) in agricultural environments. Behavioral aspects of seed selection and consumption have not been fully characterized, especially with regards to seed traits that mice select for or against. The present study used a common strain of mice (C57BL/6J) to evaluate the effect of seed coat pigmentation and hardness (texture) on wheat seed selection and consumption. Wheat grains with contrasting traits were presented to mice in binary mixtures, similar to "two-choice" tests. Mice preferred wheat grain with fewer Red pigmentation genes (similar to avoided grain with a greater number of Red genes). The mice were sensitive to small differences in grain texture, with softer texture consistently preferred. In summary, both seed color (pigmentation) and texture influence the selective consumption of wheat grain by C57BL/6J mice. Although the preference for softer texture and less pigmented grain is clear, other aspects of behavioral selection and consumption preference will require further study. C1 [Kiszonas, Alecia M.; Fuerst, E. Patrick; Morris, Craig F.] Washington State Univ, USDA ARS, Western Wheat Qual Lab, E-202 Food Qual Bldg, Pullman, WA 99164 USA. [Talbert, Luther; Sherman, Jamie] Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. RP Morris, CF (reprint author), Washington State Univ, USDA ARS, Western Wheat Qual Lab, E-202 Food Qual Bldg, Pullman, WA 99164 USA. EM morrisc@wsu.edu FU Ardent Mills LLC FX Caleb James performed the mouse feeding trials and data collection; his keen observations were invaluable to the success of this research. The staff of the WSU Vivarium are thanked. Stacey Sykes and Shawna Vogl assisted in the preparation and submission of the manuscript. Partial funding was provided by Ardent Mills LLC. NR 31 TC 0 Z9 0 U1 4 U2 4 PU WALTER DE GRUYTER GMBH PI BERLIN PA GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY SN 0025-1461 EI 1864-1547 J9 MAMMALIA JI Mammalia PD NOV PY 2016 VL 80 IS 6 BP 655 EP 662 DI 10.1515/mammalia-2015-0123 PG 8 WC Zoology SC Zoology GA EA1EU UT WOS:000386336100008 ER PT J AU von Rein, I Kayler, ZE Premke, K Gessler, A AF von Rein, I. Kayler, Z. E. Premke, K. Gessler, A. TI Desiccation of sediments affects assimilate transport within aquatic plants and carbon transfer to microorganisms SO PLANT BIOLOGY LA English DT Article DE Aquatic plant-sediment-microorganism carbon continuum; (CO2)-C-13 pulse labelling; drought stress; non-structural carbon compounds (NSCs); phospholipid fatty acids (PLFAs); Phragmites australis; stable isotopes; Typha latifolia ID COMMUNITY COMPOSITION; MICROBIAL BIOMASS; PHRAGMITES-AUSTRALIS; ISOTOPIC COMPOSITION; EXPERIMENTAL DROUGHT; TEMPERATE GRASSLAND; RICINUS-COMMUNIS; SUMMER DROUGHT; LAKE-SEDIMENTS; SOIL-MOISTURE AB With the projected increase in drought duration and intensity in future, small water bodies, and especially the terrestrial-aquatic interfaces, will be subjected to longer dry periods with desiccation of the sediment. Drought effects on the plant-sediment microorganism carbon continuum may disrupt the tight linkage between plants and microbes which governs sediment carbon and nutrient cycling, thus having a potential negative impact on carbon sequestration of small freshwater ecosystems. However, research on drought effects on the plant-sediment carbon transfer in aquatic ecosystems is scarce. We therefore exposed two emergent aquatic macrophytes, Phragmites australis and Typha latifolia, to a month-long summer drought in a mesocosm experiment. We followed the fate of carbon from leaves to sediment microbial communities with (CO2)-C-13 pulse labelling and microbial phospholipid-derived fatty acid (PLFA) analysis. We found that drought reduced the total amount of carbon allocated to stem tissues but did not delay the transport. We also observed an increase in accumulation of C-13-labelled sugars in roots and found a reduced incorporation of C-13 into the PLFAs of sediment microorganisms. Drought induced a switch in plant carbon allocation priorities, where stems received less new assimilates leading to reduced starch reserves whilst roots were prioritised with new assimilates, suggesting their use for osmoregulation. There were indications that the reduced carbon transfer from roots to microorganisms was due to the reduction of microbial activity via direct drought effects rather than to a decrease in root exudation or exudate availability. C1 [von Rein, I.; Kayler, Z. E.; Premke, K.; Gessler, A.] Leibniz Ctr Agr Landscape Res ZALF, Inst Landscape Biogeochem, Eberswalder Str 84, D-15374 Muncheberg, Germany. [Kayler, Z. E.] US Forest Serv, USDA, No Res Stn, Lawrence Livermore Natl Lab, Livermore, CA USA. [Premke, K.] Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Chem Analyt & Biogeochem, Berlin, Germany. [Gessler, A.] Berlin Brandenburg Inst Adv Biodivers Res BBIB, Berlin, Germany. [Gessler, A.] Swiss Fed Inst Forest Snow & Landscape Res WSL, Birmensdorf, Switzerland. RP von Rein, I (reprint author), Leibniz Ctr Agr Landscape Res ZALF, Inst Landscape Biogeochem, Eberswalder Str 84, D-15374 Muncheberg, Germany. EM isabell.rein@zalf.de RI Gessler, Arthur/C-7121-2008 OI Gessler, Arthur/0000-0002-1910-9589 FU German Research Council (DFG); Swiss National Science Foundation (SNF) FX Special thanks go to all those who helped with the harvests and sample preparations: Petra Lange, Monika Roth, Kennedy Kweku Kasta, Darline Krebel, Marco Heyde, Kai Nitzsche, Sasa Zavadlav and Marcus Fahle. We are grateful to Matthias Saurer from the PSI for his fast response and help with isotope analyses. We also want to thank Grit von der Waydbrink for her technical assistance in sugar analyses and Susanne Remus for her help in isotope analyses. AG acknowledges financial support from the German Research Council (DFG) and from the Swiss National Science Foundation (SNF). NR 68 TC 0 Z9 0 U1 15 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1435-8603 EI 1438-8677 J9 PLANT BIOLOGY JI Plant Biol. PD NOV PY 2016 VL 18 IS 6 BP 947 EP 961 DI 10.1111/plb.12486 PG 15 WC Plant Sciences SC Plant Sciences GA EA1KG UT WOS:000386350400009 PM 27465780 ER PT J AU Sandhu, D Atkinson, T Noll, A Johnson, C Espinosa, K Boelter, J Abel, S Dhatt, BK Barta, T Singsaas, E Sepsenwol, S Goggi, AS Palmer, RG AF Sandhu, Devinder Atkinson, Taylor Noll, Andrea Johnson, Callie Espinosa, Katherine Boelter, Jessica Abel, Stephanie Dhatt, Balpreet K. Barta, Terese Singsaas, Eric Sepsenwol, Sol Goggi, A. Susana Palmer, Reid G. TI Soybean proteins GmTic110 and GmPsbP are crucial for chloroplast development and function SO PLANT SCIENCE LA English DT Article DE Soybean; Chlorophyll-deficient; Photosynthesis; Tic110; PsbP ID CHLOROPHYLL-DEFICIENT MUTANT; IMPORT MACHINERY; OXYGEN EVOLUTION; PHOTOSYSTEM-II; HIGHER-PLANTS; BINDING-SITE; GLYCINE-MAX; GENE; LINKAGE; IDENTIFICATION AB We have identified a viable-yellow and a lethal-yellow chlorophyll-deficient mutant in soybean. Segregation patterns suggested single-gene recessive inheritance for each mutant. The viable- and lethal-yellow plants showed significant reduction of chlorophyll a and b. Photochemical energy conversion efficiency and photochemical reflectance index were reduced in the viable-yellow plants relative to the wild type, whereas the lethal-yellow plants showed no electron transport activity. The viable-yellow plants displayed reduced thylakoid stacking, while the lethal-yellow plants exhibited failure of proplastid differentiation into normal chloroplasts with grana. Genetic analysis revealed recessive epistatic interaction between the viable- and the lethal-yellow genes. The viable-yellow gene was mapped to a 58 kb region on chromosome 2 that contained seven predicted genes. A frame shift mutation, due to a single base deletion in Glyma.02g233700, resulted in an early stop codon. Glyma.02g233700 encodes a translocon in the inner membrane of chloroplast (GmTic110) that plays a critical role in plastid biogenesis. The lethal-yellow gene was mapped to an 83 kb region on chromosome 3 that contained 13 predicted genes. Based on the annotated functions, we sequenced three potential candidate genes. A single base insertion in the second exon of Glyma.03G230300 resulted in a truncated protein. Glyma.03G230300 encodes for GmPsbP, an extrinsic protein of Photosystem II that is critical for oxygen evolution during photosynthesis. GmTic110 and GmPsbP displayed highly reduced expression in the viable- and lethal-yellow mutants, respectively. The yellow phenotypes in the viable- and lethal-yellow mutants were due to the loss of function of GmTic110 or GmPsbP resulting in photooxidative stress. Published by Elsevier Ireland Ltd. C1 [Sandhu, Devinder] USDA ARS, US Salin Lab, Riverside, CA 92507 USA. [Atkinson, Taylor; Noll, Andrea; Johnson, Callie; Boelter, Jessica; Abel, Stephanie; Dhatt, Balpreet K.; Barta, Terese; Singsaas, Eric; Sepsenwol, Sol] Univ Wisconsin, Dept Biol, Stevens Point, WI 54481 USA. [Espinosa, Katherine; Goggi, A. Susana; Palmer, Reid G.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Dhatt, Balpreet K.] Punjab Agr Univ, Dept Plant Breeding & Genet, Ludhiana, Punjab, India. [Singsaas, Eric] Univ Minnesota, Nat Resources Res Inst, Duluth, MN 55811 USA. RP Sandhu, D (reprint author), 450 W Big Springs Rd, Riverside, CA 92507 USA. EM Devinder.sandhu@ars.usda.gov; tayatkinson25@gmail.com; anoll226@gmail.com; Callie.Johnson@landmark.coop; kt.espinosa67@gmail.com; jboelt92@gmail.com; sabel728@gmail.com; dhattbalpreet03@gmail.com; tbarta@uwsp.edu; esingsaa@d.umn.edu; ssepsenw@uwsp.edu; susana@iastate.edu FU UWSP Undergraduate Education Initiative; UWSP Student Research Funds; University Personnel Development Committee funds; American Seed Trade Association's Seed Research Fund FX This research project was supported by the UWSP Undergraduate Education Initiative, the UWSP Student Research Funds and University Personnel Development Committee funds. Dr. Katherine Espinosa's graduate research was supported by the American Seed Trade Association's Seed Research Fund. Authors thank Dr. Manju Pudussery for helping with expression analysis and Dr. Jorge Ferreira for taking pictures of the plants. NR 45 TC 0 Z9 0 U1 5 U2 5 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0168-9452 J9 PLANT SCI JI Plant Sci. PD NOV PY 2016 VL 252 BP 76 EP 87 DI 10.1016/j.plantsci.2016.07.006 PG 12 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA EA2GE UT WOS:000386410000008 PM 27717480 ER PT J AU Patwa, A Ambrose, RPK Casada, ME AF Patwa, Abhay Ambrose, R. P. Kingsly Casada, Mark. E. TI Discrete element method as an approach to model the wheat milling process SO POWDER TECHNOLOGY LA English DT Article ID RHEOLOGICAL PROPERTIES; COMPRESSIVE STRENGTH; DEM; GRAINS; ENDOSPERM; PARTICLE; COMMINUTION; BREAKAGE; MOISTURE AB It is a well-known phenomenon that break-release, particle size, and size distribution of wheat milling are functions of machine operational parameters and grain properties. Due to the non-uniform characteristics and properties of wheat kernels, the kernel physical and mechanical properties affect the size reduction process. This research was the first to test the functionality of the discrete element method (DEM) to simulate the 1st break wheat roller milling process. DEM models of 1st break wheat milling were developed by incorporating the bonded particle model along with the spherical-shaped and kernel-shaped particle models. The models simulated hard red winter (HRW) wheat milling at 12%, 14%, and 16% moisture content and was validated using lab scale milling trials. The spherical-shaped approach simulated the size reduction of a spherical cluster of mono-sized particles with uniform bond strength throughout the kernel. At 16% moisture content, this spherical-shaped kernel model resulted in an average particle size of 438 mu m with a deviation of prediction of 177%. The prediction error was reduced to 144% with a mean PSD of 372 mu m by modifying the shear modulus and coefficient of restitution values. With the kernel-shaped model, a bonded cluster resembling a wheat kernel in shape and size was used with a random distribution of particle bond strengths in the kernel. Even though the model predicted a 1st break particle size of 413 mu m, which had a deviation of 139% from the lab scale milling results, the model satisfactorily predicted the variation in particle size distribution from 1st break milling with moisture content. (C) 2016 Elsevier B.V. All rights reserved. C1 [Patwa, Abhay] Mennel Milling Co, Fostoria, OH 44830 USA. [Ambrose, R. P. Kingsly] Purdue Univ, Dept Agr & Biol Engn, 225 S Univ St, W Lafayette, IN 47907 USA. [Casada, Mark. E.] ARS, USDA, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. RP Ambrose, RPK (reprint author), Purdue Univ, Dept Agr & Biol Engn, 225 S Univ St, W Lafayette, IN 47907 USA. EM rambrose@purdue.edu NR 34 TC 0 Z9 0 U1 13 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0032-5910 EI 1873-328X J9 POWDER TECHNOL JI Powder Technol. PD NOV PY 2016 VL 302 BP 350 EP 356 DI 10.1016/j.powtec.2016.08.052 PG 7 WC Engineering, Chemical SC Engineering GA EA2KF UT WOS:000386420500034 ER PT J AU Wang, ZS Erb, AM Schaaf, CB Sun, QS Liu, Y Yang, Y Shuai, YM Casey, KA Roman, MO AF Wang, Zhuosen Erb, Angela M. Schaaf, Crystal B. Sun, Qingsong Liu, Yan Yang, Yun Shuai, Yanmin Casey, Kimberly A. Roman, Miguel O. TI Early spring post-fire snow albedo dynamics in high latitude boreal forests using Landsat-8 OLI data SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Landsat-8 snow albedo; Post-fire recovery; Albedo heterogeneity and dynamics ID LAND-COVER DATABASE; BURN SEVERITY; INTERIOR ALASKA; BLACK SPRUCE; SURFACE REFLECTANCE; ENERGY-EXCHANGE; PRODUCT MCD43A; CLIMATE-CHANGE; CLOUD SHADOW; IN-SITU AB Taking advantage of the improved radiometric resolution of Landsat-8 OLI which, unlike previous Landsat sensors, does not saturate over snow, the progress of fire recovery progress at the landscape scale (<100 m) is examined. High quality Landsat-8 albedo retrievals can now capture the true reflective and layered character of snow cover over a full range of land surface conditions and vegetation densities. This new capability particularly improves the assessment of post-fire vegetation dynamics across low- to high-burn severity gradients in Arctic and boreal regions in the early spring, when the albedos during recovery show the greatest variation. We use 30 m resolution Landsat-8 surface reflectances with concurrent coarser resolution (500 m) MODIS high quality full inversion surface Bidirectional Reflectance Distribution Functions (BRDF) products to produce higher resolution values of surface albedo. The high resolution full expression shortwave blue sky albedo product performs well with an overall RMSE of 0.0267 between tower and satellite measures under both snow-free and snow-covered conditions. While the importance of post-fire albedo recovery can be discerned from the MODIS albedo product at regional and global scales, our study addresses the particular importance of early spring post-fire albedo recovery at the landscape scale by considering the significant spatial heterogeneity of burn severity, and the impact of snow on the early spring albedo of various vegetation recovery types. We found that variations in early spring albedo within a single MODIS gridded pixel can be larger than 0.6. Since the frequency and severity of wildfires in Arctic and boreal systems is expected to increase in the coming decades, the dynamics of albedo in response to these rapid surface changes will increasingly impact the energy balance and contribute to other climate processes and physical feedback mechanisms. Surface radiation products derived from Landsat-8 data will thus play an important role in characterizing the carbon cycle and ecosystem processes of high latitude systems. (C) 2016 Elsevier Inc. All rights reserved. C1 [Wang, Zhuosen; Casey, Kimberly A.; Roman, Miguel O.] NASA, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. [Wang, Zhuosen; Erb, Angela M.; Schaaf, Crystal B.; Sun, Qingsong; Liu, Yan; Shuai, Yanmin] Univ Massachusetts, Sch Environm, Boston, MA 02125 USA. [Wang, Zhuosen] Goddard Space Flight Ctr, Greenbelt, MD USA. [Yang, Yun] ARS, USDA, Beltsville, MD USA. [Casey, Kimberly A.] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20742 USA. RP Wang, ZS (reprint author), NASA, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA.; Erb, AM (reprint author), Univ Massachusetts, Sch Environm, Boston, MA 02125 USA. EM zhuosen.wang@nasa.gov; Angela.Erb001@umb.edu OI Sun, Qingsong/0000-0002-7710-2123 FU NASA [NNX14A173G]; USGS [G12PC00072]; Office of Science (BER), US Department of Energy through the Ameriflux Management Project FX This research was supported by NASA awards NNX14A173G and USGS award G12PC00072. The MODIS data were obtained from the NASA Distributed Active Archive Centers (DAACs). The Landsat data were obtained from the USGS Earth Resources Observation and Science (EROS) Center Science Processing Architecture (ESPA). Field albedo measurements were downloaded from NOAA SURFRAD. We gratefully acknowledge Dr. Adrian Rocha from University of Notre Dame for providing ground albedo measurements at the Imnavait site, Dr. David Y. Hollinger from USDA Forest Service Northern Research Station, Durham, NH, USA for providing ground albedo measurements at the Howland West site and Dr. Kimberly Novick and Dr. Benjamin Sulman from Indiana University for providing ground albedo measurements at the Morgan Monroe State Forest site which was supported primarily by the Office of Science (BER), US Department of Energy through the Ameriflux Management Project. NR 91 TC 0 Z9 0 U1 15 U2 15 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD NOV PY 2016 VL 185 SI SI BP 71 EP 83 DI 10.1016/j.rse.2016.02.059 PG 13 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA EA1AY UT WOS:000386321900007 ER PT J AU Semmens, KA Anderson, MC Kustas, WP Gao, F Alfieri, JG McKee, L Prueger, JH Hain, CR Cammalleri, C Yang, Y Xia, T Sanchez, L Alsina, MM Velez, M AF Semmens, Kathryn A. Anderson, Martha C. Kustas, William P. Gao, Feng Alfieri, Joseph G. McKee, Lynn Prueger, John H. Hain, Christopher R. Cammalleri, Carmelo Yang, Yun Xia, Ting Sanchez, Luis Alsina, Maria Mar Velez, Monica TI Monitoring daily evapotranspiration over two California vineyards using Landsat 8 in a multi-sensor data fusion approach SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Landsat 8; Evapotranspiration; Vineyard; MODIS; Fusion; Energy fluxes ID SURFACE-ENERGY FLUXES; MAPPING DAILY EVAPOTRANSPIRATION; SPATIAL-RESOLUTION; MODEL PERFORMANCE; SATELLITE IMAGERY; 2-SOURCE MODEL; WATER-CONTENT; TIME-SERIES; SCALES; BALANCE AB California's Central Valley grows a significant fraction of grapes used for wine production in the United States. With increasing vineyard acreage, reduced water availability in much of California, and competing water use interests, it is critical to be able to monitor regional water use and evapotranspiration (ET) over large areas, but also in detail at individual field scales to improve water management within these viticulture production systems. This can be achieved by integrating remote sensing data from multiple satellite systems with different spatiotemporal characteristics. In this research, we evaluate the utility of a multi-scale system for monitoring ET as applied over two vineyard sites near Lodi, California during the 2013 growing season, leading into the drought in early 2014. The system employs a multi-sensor satellite data fusion methodology (STARFM: Spatial and Temporal Adaptive Reflective Fusion Model) combined with a multi-scale ET retrieval algorithm based on the Two Source Energy Balance (TSEB) land-surface representation to compute daily ET at 30 m resolution. In this system, TSEB is run using thermal band imagery from the Geostationary Environmental Operational Satellites (GOES; 4 km spatial resolution, hourly temporal sampling), the Moderate Resolution Imaging Spectroradiometer (MODIS) data (1 km resolution, daily acquisition) and the new Landsat 8 satellite (sharpened to 30 m resolution, similar to 16 day acquisition). Estimates of daily ET generated in two neighboring fields of Pinot noir vines of different age agree with ground-based flux measurements acquired in-field during most of the 2013 season with relative mean absolute errors on the order of 19-23% (root mean square errors of approximately 1 mm d(-1)), reducing to 14-20% at the weekly timestep relevant for irrigation management (similar to 5 mm wk(-1)). A model overestimation of ET in the early season was detected in the younger vineyard, perhaps relating to an inter-row grass cover crop. Spatial patterns of cumulative ET generally correspond to measured yield maps and indicate areas of variable crop moisture, soil condition, and yield within the vineyards that could require adaptive management. The results suggest that multi-sensor remote sensing observations provide a unique means for monitoring crop water use and soil moisture status at field-scales over extended growing regions, and may have value in supporting operational water management decisions in vineyards and other high value crops. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license. C1 [Semmens, Kathryn A.; Anderson, Martha C.; Kustas, William P.; Gao, Feng; Alfieri, Joseph G.; McKee, Lynn; Yang, Yun] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Prueger, John H.] USDA ARS, Natl Lab Agr & Environm, Ames, IA USA. [Hain, Christopher R.] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20742 USA. [Cammalleri, Carmelo] European Commiss, Joint Res Ctr, Inst Environm & Sustainabil, Climate Risk Management Unit, Ispra, Italy. [Xia, Ting] Tsinghua Univ, Beijing, Peoples R China. [Sanchez, Luis; Alsina, Maria Mar] E&J Gallo Winery Viticulture Res, Modesto, CA USA. [Velez, Monica] Univ Puerto Rico Rio Piedras, USDA, Hispan Serving Inst Natl Program, San Juan, PR USA. RP Semmens, KA (reprint author), USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. EM kalese@gmail.com RI Anderson, Martha/C-1720-2015 OI Anderson, Martha/0000-0003-0748-5525 FU USDA Natural Resources Career Track-Puerto Rico/USDA Hispanic Serving Institutions National Program-Preparing Students For Career Paths With The USDA Forest Service By Linking Student Success With Experiential Learning Opportunities; Ernest and Julio Gallo Winery FX We would like to acknowledge the support for the internship of Monica Velez from the USDA Natural Resources Career Track-Puerto Rico/USDA Hispanic Serving Institutions National Program-Preparing Students For Career Paths With The USDA Forest Service By Linking Student Success With Experiential Learning Opportunities. Funding provided by Ernest and Julio Gallo Winery made possible the acquisition and processing of the high resolution aircraft imagery used in this study. In addition we would like to thank the staff of Viticulture, Chemistry and Enology Division of Ernest and Julio Gallo Winery, in particular Nick Dokooozlian, Luis Sanchez, and Martin Mendez-Costabel who made possible the collection of the field validation data. The US Department of Agriculture (USDA) prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable, sex, marital status, familial status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal, or because all or part of an individual's income is derived from any public assistance program. (Not all prohibited bases apply to all programs.) Persons with disabilities who require alternative means for communication of program information (Braille, large print, audiotape, etc.) should contact USDA's TARGET Center at (202) 720-2600 (voice and TDD). To file a complaint of discrimination, write to USDA, Director, Office of Civil Rights, 1400 Independence Avenue, S.W., Washington, D.C. 20250-9410, or call (800) 795-3272 (voice) or (202) 720-6382 (TDD). USDA is an equal opportunity provider and employer. NR 65 TC 6 Z9 6 U1 33 U2 33 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD NOV PY 2016 VL 185 SI SI BP 155 EP 170 DI 10.1016/j.rse.2015.10.025 PG 16 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA EA1AY UT WOS:000386321900014 ER PT J AU Huntington, J McGwire, K Morton, C Snyder, K Peterson, S Erickson, T Niswonger, R Carroll, R Smith, G Allen, R AF Huntington, Justin McGwire, Kenneth Morton, Charles Snyder, Keirith Peterson, Sarah Erickson, Tyler Niswonger, Richard Carroll, Rosemary Smith, Guy Allen, Richard TI Assessing the role of climate and resource management on groundwater dependent ecosystem changes in arid environments with the Landsat archive SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Landsat; NDVI; Cross-sensor calibration; Groundwater dependent ecosystems; Riparian restoration; Groundwater pumping; Phreatophytes; Complementary relationship; Evaporative demand ID WATER-TABLE DRAWDOWN; GREATER SAGE-GROUSE; RIPARIAN VEGETATION; SURFACE REFLECTANCE; FOREST DISTURBANCE; DETECTING TRENDS; EVAPOTRANSPIRATION; MODEL; BASIN; RESTORATION AB Groundwater dependent ecosystems (GDEs) rely on near-surface groundwater. These systems are receiving more attention with rising air temperature, prolonged drought, and where groundwater pumping captures natural groundwater discharge for anthropogenic use. Phreatophyte shrublands, meadows, and riparian areas are GDEs that provide critical habitat for many sensitive species, especially in arid and semi-arid environments. While GDEs are vital for ecosystem services and function, their long-term (i.e. similar to 30 years) spatial and temporal variability is poorly understood with respect to local and regional scale climate, groundwater, and rangeland management. In this work, we compute time series of NDVI derived from sensors of the Landsat TM, ETM +, and OLI lineage for assessing GDEs in a variety of land and water management contexts. Changes in vegetation vigor based on climate, groundwater availability, and land management in arid landscapes are detectable with Landsat. However, the effective quantification of these ecosystem changes can be undermined if changes in spectral bandwidths between different Landsat sensors introduce biases in derived vegetation indices, and if climate, and land and water management histories are not well understood. The objective of this work is to 1) use the Landsat 8 under-fly dataset to quantify differences in spectral reflectance and NDVI between Landsat 7 ETM + and Landsat 8 OLI for a range of vegetation communities in arid and semiarid regions of the southwestern United States, and 2) demonstrate the value of 30-year historical vegetation index and climate datasets for assessing GDEs. Specific study areas were chosen to represent a range of GDEs and environmental conditions important for three scenarios: baseline monitoring of vegetation and climate, riparian restoration, and groundwater level changes. Google's Earth Engine cloud computing and environmental monitoring platform is used to rapidly access and analyze the Landsat archive along with downscaled North American Land Data Assimilation System gridded meteorological data, which are used for both atmospheric correction and correlation analysis. Results from the cross-sensor comparison indicate a benefit from the application of a consistent atmospheric correction method, and that NDVI derived from Landsat 7 and 8 are very similar within the study area. Results from continuous Landsat time series analysis clearly illustrate that there are strong correlations between changes in vegetation vigor, precipitation, evaporative demand, depth to groundwater, and riparian restoration. Trends in summer NDVI associated with riparian restoration and groundwater level changes were found to be statistically significant, and interannual summer NDVI was found to be moderately correlated to interannual water-year precipitation for baseline study sites. Results clearly highlight the complementary relationship between water-year PPT, NDVI, and evaporative demand, and are consistent with regional vegetation index and complementary relationship studies. This work is supporting land and water managers for evaluation of GDEs with respect to climate, groundwater, and resource management. (C) 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license. C1 [Huntington, Justin; McGwire, Kenneth; Morton, Charles; Carroll, Rosemary; Smith, Guy] Desert Res Inst, Reno, NV USA. [Huntington, Justin] Western Reg Climate Ctr, Reno, NV 89512 USA. [Snyder, Keirith] USDA ARS, Reno, NV USA. [Peterson, Sarah] Bur Land Management, Reno, NV USA. [Erickson, Tyler] Google Inc, Mountain View, CA USA. [Niswonger, Richard] US Geol Survey, 345 Middlefield Rd, Menlo Pk, CA 94025 USA. [Allen, Richard] Univ Idaho, Kimberly, ID USA. RP Huntington, J (reprint author), Western Reg Climate Ctr, Reno, NV 89512 USA. EM justin.huntington@dri.edu OI Smith, Guy/0000-0002-4579-8752 FU U.S. Bureau of Land Management [L13AC00169]; U.S. Department of Agriculture Agricultural Research Service [59-5370-3-001]; Desert Research Institute Maki Endowment; U.S. Geological Survey Landsat Science Team; Department of the Interior Northwest Climate Science Center grant; Google Earth Engine faculty research grant FX Funding for this study was provided by the U.S. Bureau of Land Management grant #L13AC00169, U.S. Department of Agriculture Agricultural Research Service grant #59-5370-3-001, Desert Research Institute Maki Endowment, U.S. Geological Survey 2012-2017 Landsat Science Team, Department of the Interior Northwest Climate Science Center grant to evaluate new strategies for addressing climate change impacts on water and ecosystems in the Great Basin, and a Google Earth Engine faculty research grant. ESPA Landsat Surface Reflectance products used were courtesy of the U.S. Geological Survey. The authors would like to thank Stephen Maples of the U.S. Geological Survey, and four anonymous reviewers for their thoughtful comments and suggestions. They greatly improved the manuscript. NR 70 TC 2 Z9 2 U1 27 U2 27 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD NOV PY 2016 VL 185 SI SI BP 186 EP 197 DI 10.1016/j.rse.2016.07.004 PG 12 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA EA1AY UT WOS:000386321900016 ER PT J AU Schroeder, W Oliva, P Giglio, L Quayle, B Lorenz, E Morelli, F AF Schroeder, Wilfrid Oliva, Patricia Giglio, Louis Quayle, Brad Lorenz, Eckehard Morelli, Fabiano TI Active fire detection using Landsat-8/OLI data SO REMOTE SENSING OF ENVIRONMENT LA English DT Article ID WILDLAND-URBAN INTERFACE; WESTERN UNITED-STATES; RADIATIVE ENERGY; ETM PLUS; MODIS; PRODUCT; VALIDATION; SYSTEM; ASTER; ALGORITHM AB The gradual increase in Landsat-class data availability creates new opportunities for fire science and management applications that require higher-fidelity information about biomass burning, improving upon existing coarser spatial resolution (>= 1 km) satellite active fire data sets. Targeting those enhanced capabilities we describe an active fire detection algorithm for use with Landsat-8 Operational Land Imager (OLI) daytime and nighttime data. The approach builds on the fire-sensitive short-wave infrared channel 7 complemented by visible and near-infrared channel 1-6 data (daytime only), while also expanding on the use of multi-temporal analysis to improve pixel classification results. Despite frequent saturation of OLI's fire-affected pixels, which includes radiometric artifacts resulting from folding of digital numbers, our initial assessment based on visual image analysis indicated high algorithm fidelity across a wide range of biomass burning scenarios, gas flares and active volcanoes. Additional field data verification confirmed the sensor's and algorithm's ability to resolve fires of significantly small areas compared to current operational satellite fire products. Commission errors were greatly reduced with the addition of multi-temporal analysis tests applied toco-located pixels, averaging less than 0.2% globally. Because of its overall quality, Landsat-8/OLI active fire data could become part of a network of emerging earth observation systems providing enhanced spatial and temporal coverage of biomass burning at global scales. (C) 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license. C1 [Schroeder, Wilfrid; Oliva, Patricia; Giglio, Louis] Univ Maryland, Dept Geog Sci, 2181 LeFrak Hall, College Pk, MD 20742 USA. [Quayle, Brad] US Forest Serv, USDA, Remote Sensing Applicat Ctr, Salt Lake City, UT USA. [Lorenz, Eckehard] German Aerosp Ctr, Inst Opt Sensor Syst, Berlin, Germany. [Morelli, Fabiano] Brazilian Inst Space Res, Sao Jose Dos Campos, Brazil. RP Schroeder, W (reprint author), Univ Maryland, Dept Geog Sci, 2181 LeFrak Hall, College Pk, MD 20742 USA. EM wschroed@umd.edu RI Schroeder, Wilfrid/F-6738-2010 FU NASA Applied Sciences Program [NNX12AQ87G]; USDA Forest Service Remote Sensing Applications Center; GOES-R Algorithm Working Group at NOAA's National Environmental Satellite, Data, and Information Service FX We thank DLR, Erik Borg, and Doris Klein for hosting and sharing data from the small biomass burning experiment in Demmin/Germany on 17 August 2013. We also thank INPE for hosting the small biomass burning experiment on 19 January 2015 in Cachoeira Paulista/Brazil, and Andre Lima for sharing field data. Funding support for this study was provided by the NASA Applied Sciences Program (grant #NNX12AQ87G), the USDA Forest Service Remote Sensing Applications Center, and the GOES-R Algorithm Working Group at NOAA's National Environmental Satellite, Data, and Information Service. We thank four anonymous reviewers for their comments. NR 48 TC 2 Z9 2 U1 19 U2 19 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD NOV PY 2016 VL 185 SI SI BP 210 EP 220 DI 10.1016/j.rse.2015.08.032 PG 11 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA EA1AY UT WOS:000386321900018 ER PT J AU Wulder, MA White, JC Loveland, TR Woodcock, CE Belward, AS Cohen, WB Fosnight, EA Shaw, J Masek, JG Roy, DP AF Wulder, Michael A. White, Joanne C. Loveland, Thomas R. Woodcock, Curtis E. Belward, Alan S. Cohen, Warren B. Fosnight, Eugene A. Shaw, Jerad Masek, Jeffrey G. Roy, David P. TI The global Landsat archive: Status, consolidation, and direction SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Landsat Global Archive Consolidation; LGAC; Satellite; Monitoring; Landsat ID TERM ACQUISITION PLAN; COVER; RESOLUTION; MISSION; IMAGERY; AVAILABILITY; CONTINUITY; SATELLITE; ACCESS; RECORD AB New and previously unimaginable Landsat applications have been fostered by a policy change in 2008 that made analysis-ready Landsat data free and open access. Since 1972, Landsat has been collecting images of the Earth, with the early years of the program constrained by onboard satellite and ground systems, as well as limitations across the range of required computing, networking, and storage capabilities. Rather than robust on-satellite storage for transmission via high bandwidth downlink to a centralized storage and distribution facility as with Landsat-8, a network of receiving stations, one operated by the U.S. government, the other operated by a community of International Cooperators (ICs), were utilized. ICs paid a fee for the right to receive and distribute Landsat data and over time, more Landsat data was held outside the archive of the United State Geological Survey (USGS) than was held inside, much of it unique. Recognizing the critical value of these data, the USGS began a Landsat Global Archive Consolidation (LGAC) initiative in 2010 to bring these data into a single, universally accessible, centralized global archive, housed at the Earth Resources Observation and Science (EROS) Center in Sioux Falls, South Dakota. The primary LGAC goals are to inventory the data held by ICs, acquire the data, and ingest and apply standard ground station processing to generate an LIT analysis-ready product. As of January 1, 2015 there were 5,532,454 images in the USGS archive. LGAC has contributed approximately 3.2 million of those images, more than doubling the original USGS archive holdings. Moreover, an additional 23 million images have been identified to date through the LGAC initiative and are in the process of being added to the archive. The impact of LGAC is significant and, in terms of images in the collection, analogous to that of having had two additional Landsat-5 missions. As a result of LGAC, there are regions of the globe that now have markedly improved Landsat data coverage, resulting in an enhanced capacity for mapping, monitoring change, and capturing historic conditions. Although future missions can be planned and implemented, the past cannot be revisited, undetscoring the value and enhanced significance of historical Landsat data and the LGAC initiative. The aim of this paper is to report the current status of the global USGS Landsat archive, document the existing and anticipated contributions of LGAC to the archive, and characterize the current acquisitions of Landsat-7 and Landsat-8. Landsat-8 is adding data to the archive at an unprecedented rate as nearly all terrestrial images are now collected. We also offer key lessons learned so far from the LGAC initiative, plus insights regarding other critical elements of the Landsat program looking forward, such as acquisition, continuity, temporal revisit, and the importance of continuing to operationalize the Landsat program. Crown Copyright (C) 2015 Published by Elsevier Inc All rights reserved. C1 [Wulder, Michael A.; White, Joanne C.] Nat Resources Canada, Canadian Forest Serv, Pacific Forestry Ctr, 506 West Burnside Rd, Victoria, BC V8Z 1M5, Canada. [Loveland, Thomas R.; Fosnight, Eugene A.] US Geol Survey, Earth Resources Observat & Sci EROS Ctr, 47914 252nd St, Sioux Falls, SD 57198 USA. [Woodcock, Curtis E.] Boston Univ, Dept Earth & Environm, Boston, MA 02215 USA. [Belward, Alan S.] European Commiss, Joint Res Ctr, Inst Environm & Sustainabil, I-20133 Milan, VA, Italy. [Cohen, Warren B.] US Forest Serv, Forestry Sci Lab, USDA, Corvallis, OR 97331 USA. [Shaw, Jerad] Stinger Ghaffarian Technol, Earth Resources Observat & Sci EROS Ctr, 47914 252nd St, Sioux Falls, SD 57198 USA. [Masek, Jeffrey G.] NASA, Biospher Sci Lab, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. [Roy, David P.] South Dakota State Univ, Geospatial Sci Ctr Excellence, Brookings, SD 57007 USA. RP Wulder, MA (reprint author), Nat Resources Canada, Canadian Forest Serv, Pacific Forestry Ctr, 506 West Burnside Rd, Victoria, BC V8Z 1M5, Canada. EM mike.wulder@canada.ca OI White, Joanne/0000-0003-4674-0373; Wulder, Michael/0000-0002-6942-1896 FU Canadian Space Agency (CSA) Government Related Initiatives Program (GRIP); Canadian Forest Service (CFS) of Natural Resources Canada FX This communication has been made possible and enriched by the ongoing discussions and deliberations of the USGS/NASA Landsat Science Team (https://landsat.usgs.gov/science_Landsat_Science_Team.php). The participation of Wulder and White was supported as part of the "National Terrestrial Ecosystem Monitoring System (NTEMS): Timely and detailed national cross-sector monitoring for Canada" project jointly funded by the Canadian Space Agency (CSA) Government Related Initiatives Program (GRIP) and the Canadian Forest Service (CFS) of Natural Resources Canada. We greatly appreciate the time committed and the insightful comments made by three anonymous reviewers that helped us to improve the manuscript. NR 46 TC 11 Z9 11 U1 10 U2 10 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD NOV PY 2016 VL 185 SI SI BP 271 EP 283 DI 10.1016/j.rse.2015.11.032 PG 13 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA EA1AY UT WOS:000386321900023 ER PT J AU Rodrigues, WP Machado, JA da Silva, JR Figueiredo, FAMMD Ferraz, TM Ferreira, LS Bezerra, LBD de Abreu, DP Bernado, WD Passos, LC de Sousa, EF Glenn, DM Ramalho, JC Campostrini, E AF Rodrigues, Weverton Pereira Machado Filho, Jose Altino da Silva, Jefferson Rangel Moura de Assis Figueiredo, Fabio Afonso Mazzei Ferraz, Tiago Massi Ferreira, Luciene Souza da Silva Bezerra, Luan Baritiello de Abreu, Deivisson Pelegrino Bernado, Wallace de Paula Passos, Leticia Cespom de Sousa, Elias Fernandes Glenn, David Michael Ramalho, Jose Cochicho Campostrini, Eliemar TI Whole-canopy gas exchanges in Coffea sp is affected by supra-optimal temperature and light distribution within the canopy: The insights from an improved multi-chamber system SO SCIENTIA HORTICULTURAE LA English DT Article DE Climate change; Heat stress; Photosynthesis; Whole plant; Coffee tree ID CLIMATE-CHANGE; STOMATAL CONDUCTANCE; NET PHOTOSYNTHESIS; PLANT RESPIRATION; PARTICLE FILMS; ARABICA; LEAF; CO2; YIELD; FIELD AB Whole-canopy gas exchange provides a more accurate measurement of CO2 assimilation (A(c)) and transpiration (E-c) compared to single-leaf. The objectives of this study were to evaluate whole-canopy gas exchange of 2 coffee species: Coffea arabica cv. Catuai Amarelo-Catuai and Coffea canephora cv. Emcapa 8111 Clone 02-Clone 02 during two different seasons varying in temperature. Six plants >1-year-old of each species were grown in pots (100L) in a greenhouse. Soil moisture was maintained at field capacity. Data were continuously collected for 10 days during spring (September 2014 moderate temperatures) and summer (February 2015 with high temperature) and micrometeorological variables were monitored inside the greenhouse. Catuai had a lower area leaf and higher branch angle resulting in greater light distribution within the canopy that contributed to higher net photosynthesis and transpiration as compared to Clone 02 in both seasons. Catuai had reduced whole-canopy CO2 assimilation and transpiration during summer, mainly linked to reduced whole-canopy conductance. However, Clone 02 had similar whole-canopy CO2 assimilation and transpiration values in both seasons. Despite reduced gas exchange for Catuali during summer, the relationship between A(c) and E-c indicated that Catual had a greater instantaneous WUEC than Clone 02 during spring and there were no differences between species during summer. The dense canopy architecture of Clone 02 limited whole-canopy gas exchange due to poor light distribution within the canopy whereas Catual had better canopy light distribution resulting in higher photosynthetic rates than Clone 02 during spring. Additionally, the architecture had a negative effect when Catual experiences increased temperature that reduced whole-canopy conductance. High temperatures may limit the growth and production of coffee plants in tropical areas. (C) 2016 Elsevier B.V. All rights reserved. C1 [Rodrigues, Weverton Pereira; Machado Filho, Jose Altino; da Silva, Jefferson Rangel; Ferreira, Luciene Souza; da Silva Bezerra, Luan Baritiello; de Abreu, Deivisson Pelegrino; Bernado, Wallace de Paula; Passos, Leticia Cespom; de Sousa, Elias Fernandes; Campostrini, Eliemar] UENF, Ctr Ciencias & Tecnol Agr, Setor Fisiol Vegetal, BR-28013602 Darcy Ribeiro, RJ, Brazil. [Machado Filho, Jose Altino] Inst Capixaba Pesquisa Assistencia Tecn & Extensa, BR-29052010 Vitoria, ES, Brazil. [Moura de Assis Figueiredo, Fabio Afonso Mazzei; Ferraz, Tiago Massi] Univ Estadual Maranhao, Ctr Estudos Super Imperatriz, Rua GodofredoViana 1300, BR-65901480 Imperatriz, MA, Brazil. [Glenn, David Michael] ARS, USDA, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. [Ramalho, Jose Cochicho] ULisboa, Grp Interacoes Planta Ambiente & Biodiversidade P, Dept Recursos Nat Ambiente & Terr DRAT, Linking Landscape Environm Agr & Food LEAF,ISA, Ave Replibl, P-2784505 Oeiras, Portugal. [Ramalho, Jose Cochicho] UNL, FCT, GeoBioTec, P-2829516 Caparica, Portugal. RP Campostrini, E (reprint author), UENF, Ctr Ciencias & Tecnol Agr, Setor Fisiol Vegetal, BR-28013602 Darcy Ribeiro, RJ, Brazil. EM campostenator@gmail.com RI Rodrigues, Weverton/H-4296-2016; OI Rodrigues, Weverton/0000-0002-9137-474X; Campostrini, Eliemar/0000-0002-1329-1084; Ramalho, Jose/0000-0002-7639-7214 FU Fundacao Carlos Chagas de Apoio a Pesquisa do Estado do Rio de Janeiro (FAPERJ); Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) FX The authors wish to thank Dr. F.M. DaMatta for helpful discussions and critical reading of the manuscript. Funding for this research has been provided through grants from Fundacao Carlos Chagas de Apoio a Pesquisa do Estado do Rio de Janeiro (FAPERJ) to W.P.R. Financial support has been provided from Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq). NR 55 TC 0 Z9 0 U1 10 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD NOV 1 PY 2016 VL 211 BP 194 EP 202 DI 10.1016/j.scienta.2016.08.022 PG 9 WC Horticulture SC Agriculture GA EA2GC UT WOS:000386409800024 ER PT J AU Negron, JF McMillin, J Sieg, CH Fowler, JF Allen, KK Wadleigh, LL Anhold, JA Gibson, KE AF Negron, Jose F. McMillin, Joel Sieg, Carolyn H. Fowler, James F. Allen, Kurt K. Wadleigh, Linda L. Anhold, John A. Gibson, Ken E. TI Variables associated with the occurrence of Ips beetles, red turpentine beetle and wood borers in live and dead ponderosa pines with post-fire injury SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Dendroctonus valens; fire injury; Ips beetles; Ips spp.; Pinus ponderosa; wood borers ID FUEL-REDUCTION TREATMENTS; MORTALITY FOLLOWING FIRE; TREE MORTALITY; BARK BEETLES; NORTHERN ARIZONA; PRESCRIBED FIRE; SIERRA-NEVADA; CONIFER MORTALITY; PINUS-PONDEROSA; EASTERN OREGON AB 1 Recently, wildfires and prescribed burning have become more frequent in conifer forests of western North America. 2 Most studies examining the impacts of insects on trees with post-fire injury have focused on contributions to tree mortality. Few studies have examined fire-caused injuries to estimate the probability of attack by insects. Scant data quantifying insect associations with one another, or with live and dead fire-injured trees, are available. 3 We examined live and dead trees with varying levels of fire injury in wildfires in Colorado, Montana, Arizona and the Black Hills aiming to determine fire injury associated with insect infestation, co-occurrence between insects and insect association with live and dead fire-injured trees. 4 Bole scorch height estimated the likelihood of attack by Ips spp. Diameter at breast height, bole scorch height and crown scorch height estimated the likelihood of attack by Dendroctonus valens LeConte. Diameter at breast height and bole scorch height estimated the likelihood of attack by wood borers. 5 Ips spp., Dendroctonus valens and wood borers were associated with one another. Ips spp. beetles and wood borers were associated with dead fire-injured trees, whereas D. valens was often associated with live fire-injured trees. 6 Focusing on certain fire-caused injuries may identify trees targeted by Ips spp. beetles, Dendroctonus valens and wood borers. C1 [Negron, Jose F.] US Forest Serv, USDA, Rocky Mt Res Stn, 240 West Prospect, Ft Collins, CO 80526 USA. [McMillin, Joel] US Forest Serv, USDA, Forest Hlth Protect, Reg 4,1249 South Vinnell Way,Suite 200, Boise, ID 83709 USA. [Sieg, Carolyn H.; Fowler, James F.] US Forest Serv, USDA, Rocky Mt Res Stn, 2500 South Pine Knoll Dr, Flagstaff, AZ 86001 USA. [Allen, Kurt K.] US Forest Serv, USDA, Forest Hlth Protect, Reg 2,8221 South Highway 16, Rapid City, SD 57702 USA. [Wadleigh, Linda L.] US Forest Serv, USDA, Fire Management, Reg 3,1824 South Thompson St, Flagstaff, AZ 86001 USA. [Anhold, John A.] US Forest Serv, USDA, Forest Hlth Protect, Reg 3,2500 South Pine Knoll Dr, Flagstaff, AZ 86001 USA. [Gibson, Ken E.] US Forest Serv, USDA, Forest Hlth Protect, Reg 1,200 East Broadway, Missoula, MT 59801 USA. RP Negron, JF (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, 240 West Prospect, Ft Collins, CO 80526 USA. EM jnegron@fs.fed.us FU USDA FX Funding for this research was provided by USDA. Forest Service, Forest Health Protection, Special Technology Development Program, Project R2-2001-01, Region 1 Forest Health Protection, Region 2 Forest Health Management, Region 3 Forest Health Protection and the Rocky Mountain Research Station. We are grateful to Chad Hoffman, Noah Barstatis, Amy Uhlenhopp, John Popp, Dan Long, Denise Hardesty, Kathy Sullivan, Cassie McCraw, Laura Kaye, Matt Jedra, Brian Howell, Steve McKelvey, Kelly Williams, Joleen Atencio, Stacy Marlatt, Jason Watkins and Kelly Mogab for assisting with the data collection and entry. We thank Dave Turner, Laurie Porth and Scott Baggett for statistical support. The thoughtful reviews by three anonymous reviewers substantially improved previous versions of the manuscript. NR 70 TC 0 Z9 0 U1 6 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD NOV PY 2016 VL 18 IS 4 BP 313 EP 326 DI 10.1111/afe.12163 PG 14 WC Entomology SC Entomology GA DZ1SI UT WOS:000385619600001 ER PT J AU Haavik, LJ Dodds, KJ Ryan, K Allison, JD AF Haavik, Laurel J. Dodds, Kevin J. Ryan, Kathleen Allison, Jeremy D. TI Evidence that the availability of suitable pine limits non-native Sirex noctilio in Ontario SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Bottom-up effects; invasive species; natural enemies; population dynamics; top-down effects; wood borer ID BORER COLEOPTERA BUPRESTIDAE; BRONZE BIRCH BORER; WOODWASPS HYMENOPTERA-SIRICIDAE; NORTHEASTERN UNITED-STATES; BIOLOGICAL-CONTROL; NORTH-AMERICA; BEETLE COLEOPTERA; NATURAL ENEMIES; ECOLOGY; MANAGEMENT AB 1 Natural controls may not be effective in limiting non-native species that invade a new environment. Alternatively, factors present in the new environment can prevent an invader from becoming a serious pest. 2 We investigated whether existing natural enemies or the availability of susceptible and suitable host plants limited an established, non-native species Sirex noctilio F. (Siricidae: Hymenoptera). 3 Using several pine forests throughout Ontario, we examined the relationships between parasitism and S. noctilio activity, as well as S. noctilio activity and the availability of susceptible and suitable pine. We also evaluated parasitism at two points in time (5-7 years apart). 4 We found correlative evidence indicating that the availability of suitable hosts limited the S. noctilio population in Ontario. Sirex noctilio killed more pine when more pine was available, especially suppressed (highly suitable) pine. 5 More co-dominant (healthy) pines were attacked but not killed by S. noctilio at sites with more S. noctilio activity, indicating that higher densities of S. noctilio were needed to overwhelm healthy pines. 6 The role of parasitoids was less clear. Parasitism did not vary consistently with the amount of S. noctilio activity at sites. The two groups of parasitoids, Rhyssa and Ibalia, likely responded differently to presence of S. noctilio. C1 [Haavik, Laurel J.; Allison, Jeremy D.] Nat Resources Canada Canadian Forest Serv, Great Lakes Forestry Ctr, 1219 Queen St E, Sault Ste Marie, ON P6A 2E5, Canada. [Dodds, Kevin J.] US Forest Serv, USDA, Forest Hlth Protect, 271 Mast Rd, Durham, NH 03824 USA. [Ryan, Kathleen; Allison, Jeremy D.] Univ Toronto, Fac Forestry, 33 Willcocks St, Toronto, ON M5S 3B3, Canada. [Haavik, Laurel J.] Univ Kansas, Dept Ecol & Evolutionary Biol, 1200 Sunnyside Ave, Lawrence, KS 66046 USA. [Ryan, Kathleen] Silv Econ Ltd, 913 Southwind Court, Newmarket, ON L3Y 6J1, Canada. RP Haavik, LJ (reprint author), Nat Resources Canada Canadian Forest Serv, Great Lakes Forestry Ctr, 1219 Queen St E, Sault Ste Marie, ON P6A 2E5, Canada.; Haavik, LJ (reprint author), Univ Kansas, Dept Ecol & Evolutionary Biol, 1200 Sunnyside Ave, Lawrence, KS 66046 USA. EM ljhaavik@gmail.com OI Haavik, Laurel/0000-0002-7749-9095 FU Natural Resources Canada; Ontario Ministry of Natural Resources; USDA Forest Service-Forest Health Protection FX We thank Ronald Fournier for producing Fig. 1; Sarah Drabble, Reg Nott, Nick Boyonoski, Sarah Crispell, Katelyn Kotska, Chanel Fall, Cydney Mihell, Brett Marshall, Shannon Escasa and Elder Batista for field and laboratory assistance; Al Foley for access to the Ontario Tree Seed Facility; and four anonymous reviewers for their helpful comments on the manuscript. This work was funded in part by Natural Resources Canada, The Ontario Ministry of Natural Resources and the USDA Forest Service-Forest Health Protection. NR 60 TC 0 Z9 0 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD NOV PY 2016 VL 18 IS 4 BP 357 EP 366 DI 10.1111/afe.12167 PG 10 WC Entomology SC Entomology GA DZ1SI UT WOS:000385619600005 ER PT J AU Lopez-Martinez, V Sanchez-Martinez, G Jimenez-Garcia, D Perez-De la O, NB Coleman, TW AF Lopez-Martinez, Victor Sanchez-Martinez, Guillermo Jimenez-Garcia, Daniel Perez-De la O, Nidia B. Coleman, Tom W. TI Environmental suitability for Agrilus auroguttatus (Coleoptera: Buprestidae) in Mexico using MaxEnt and database records of four Quercus (Fagaceae) species SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Ecological niche; exotic wood borer; goldspotted oak borer; Mexican biogeographical provinces ID GOLDSPOTTED OAK BORER; SOUTHERN CALIFORNIA; FLORISTIC COMPOSITION; COMMUNITY STRUCTURE; COXALIS WATERHOUSE; DISTRIBUTIONS; WOODLANDS; PERFORMANCE; AGRIFOLIA; FORESTS AB 1 The goldspotted oak borer Agrilus auroguttatus Schaeffer is an invasive pest of three Quercus species in southern California, U.S.A. As a native and potentially indigenous exotic species, its distribution and potential damage is largely unknown in Mexico. 2 We used the maximum entropy algorithm to determine the bioclimatic variables that may explain the distribution of A. auroguttatus, as well as its interaction with the distribution of known hosts in Mexico. 3 Our model calculated high suitability for A. auroguttatus in the biogeographical provinces of California, the northern and southern areas of Sierra Madre Occidental and the northwestern area of the Mexican Plateau; moderate suitability was projected in the Baja California, Sonora and Sierra Madre Occidental provinces. Potential distribution of A. auroguttatus was overlapping with native ranges of Quercus agrifolia, Quercus emoryi Torrey and Quercus hypoleucoides A. Camus. Precipitation of the coldest quarter (23.6%), isothermality (23.5%), precipitation seasonality (17.3%) and mean temperature of the coldest quarter (11.2%) contributed most to explaining its distribution in Mexico. 4 The geographical interaction (i.e. the likelihood that the habitat would be suitable for coexistence) of A. auroguttatus with its hosts in Mexico was projected in the California province (46 382.60 km(2), probably as an exotic species) and the northwestern areas of Mexican Plateau and Sierra Madre Occidental provinces (74 013.45 km(2), probably as native). 5 Determining the areas of environmental suitability for A. auroguttatus and its host distribution can focus ground surveys, contribute to management efforts and assist in the development of risk assessments for Mexico. C1 [Lopez-Martinez, Victor; Perez-De la O, Nidia B.] Univ Autonoma Estado Morelos, Fac Ciencias Agr, Ave Chamilpa 1001, Cuernavaca 62209, Morelos, Mexico. [Sanchez-Martinez, Guillermo] INIFAP CIRNOC Campo Expt Pabellon, Programa Invest Sanidad Forestal & Agr, Km 32-5 Ctra Aguascalientes Zacatecas, Pabellon De Arteaga 20670, Mexico. [Jimenez-Garcia, Daniel] Benemerita Univ Autonoma Puebla, Inst Ciencias, Ctr Agroecol & Ambiente, 12 Sul 6301, Col San Manuel 72570, Puebla, Mexico. [Coleman, Tom W.] US Forest Serv, USDA, Forest Hlth Protect, 602 S Tippecanoe Ave, San Bernardino, CA 92408 USA. RP Lopez-Martinez, V (reprint author), Univ Autonoma Estado Morelos, Fac Ciencias Agr, Ave Chamilpa 1001, Cuernavaca 62209, Morelos, Mexico. EM victor.lopez@uaem.mx FU Programa de Fortalecimiento de la Calidad en Instituciones Educativas (PROFOCIES); Programa para el Desarrollo Profesional Docente en Educacion Superior [DSA/103.5/16/2609]; CONACYT [260517]; USDA Forest Service, Forest Health Protection, Pacific Southwest Region FX This work was partially supported by the Programa de Fortalecimiento de la Calidad en Instituciones Educativas (PROFOCIES 2014) and Programa para el Desarrollo Profesional Docente en Educacion Superior (DSA/103.5/16/2609). Nidia Belgica Perez-De la O received a graduate grant (CONACYT No. 260517). T. W. Coleman was supported by USDA Forest Service, Forest Health Protection, Pacific Southwest Region. NR 73 TC 0 Z9 0 U1 21 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD NOV PY 2016 VL 18 IS 4 BP 409 EP 418 DI 10.1111/afe.12174 PG 10 WC Entomology SC Entomology GA DZ1SI UT WOS:000385619600010 ER PT J AU Hergert, GW Margheim, JF Pavlista, AD Martin, DL Isbell, TA Supalla, RJ AF Hergert, G. W. Margheim, J. F. Pavlista, A. D. Martin, D. L. Isbell, T. A. Supalla, R. J. TI Irrigation response and water productivity of deficit to fully irrigated spring camelina SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Biodiesel; Limited irrigation; High plains aquifer; Ground; Water decline ID LIMITED IRRIGATION; CROPPING SYSTEMS; WESTERN NEBRASKA; OILSEED CROPS; HIGH-PLAINS; SATIVA OIL; CANOLA; GROWTH; YIELD; FUEL AB Camelina [Camelina sativa L Crantz] is an oil seed crop that could be adapted to the northern High Plains of the USA as a biofuel crop. Decreased ground water allocations in Nebraska necessitated determining the impact of limited irrigation on camelina. The objective of this research was to determine the effects of a range of irrigation levels on camelina yield, oil content, soil water changes and water productivity. The study was conducted for four growing seasons at two locations in western Nebraska. One site had a sandy soil whereas the other was a silt loam. Camelina was planted in early to mid-April. Cumulative irrigation treatments were 0, 100, 200, and 300 mm with the highest rate adjusted to be non-ET limiting. Camelina extracted soil water from 0.9 to 1 m depths which was shallower than canola. It showed significant response to irrigation during dry years but no response in above-average precipitation years. A water use efficiency of 7.0 kg mm(-1) with 125 mm ETc required to produce the first unit of seed yield was shown. Camelina seed yield ranged from 428 to 2867 kg ha(-1) with 187 and 536 mm of cumulative ET. In 2007 and 2008 camelina exhibited peak values in water consumed at 9-10 weeks after planting. Deficit irrigation reduced ET and yield plus accelerated maturity in those years. Oil content was increased by irrigation during drier years with no effect with high growing season precipitation. Oil content ranged from 25 to 50% depending on year, irrigation level and disease. Downey mildew significantly reduced oil content during 2009. Deficit irrigated camelina could be an alternative biofuel crop for this region but further genetic improvement would enhance its competiveness with spring canola. (C) 2016 Elsevier B.V. All rights reserved. C1 [Hergert, G. W.; Margheim, J. F.; Pavlista, A. D.] Univ Nebraska Lincoln, PREC, Scottsbluff, NE 69361 USA. [Martin, D. L.; Supalla, R. J.] Univ Nebraska, Lincoln, NE 68583 USA. [Isbell, T. A.] USDA ARS, Peoria, IL 61604 USA. RP Hergert, GW (reprint author), Univ Nebraska Lincoln, PREC, Scottsbluff, NE 69361 USA. EM ghergert1@unl.edu OI Hergert, Gary/0000-0001-6944-6199 FU USDA Risk Management Agency FX Funding for this project was provided by the USDA Risk Management Agency under several contracts. Without their support we could not have completed this research. NR 50 TC 0 Z9 0 U1 10 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD NOV PY 2016 VL 177 BP 46 EP 53 DI 10.1016/j.agwat.2016.06.009 PG 8 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DY7PY UT WOS:000385322900005 ER PT J AU Moriasi, DN King, KW Bosch, DD Bjorneberg, DL Teet, S Guzman, JA Williams, MR AF Moriasi, Daniel N. King, Kevin W. Bosch, David D. Bjorneberg, Dave L. Teet, Stephen Guzman, Jorge A. Williams, Mark R. TI Framework to parameterize and validate APEX to support deployment of the nutrient tracking tool SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Agricultural policy environmental eXtender (APEX); Nutrient tracking tool (NTT); Sensitivity analysis; Uncertainty analysis; Water quality; Water quantity ID WATER-QUALITY MODELS; AGRICULTURAL POLICY/ENVIRONMENTAL EXTENDER; MANAGEMENT STRATEGIES; HYDROLOGIC-MODELS; CALIBRATION; UNCERTAINTY; SENSITIVITY; PRODUCTIVITY; SIMULATION; GUIDELINES AB The Agricultural Policy Environmental eXtender (APEX) model is the scientific basis for the Nutrient Tracking Tool (Nu). NTT is an enhanced version of the Nitrogen Trading Tool, a user-friendly web-based computer program originally developed by the USDA. NTT was developed to estimate reductions in nutrient losses to the environment associated with alternative practices. The relatively easy access and ease with which the interface can be used has provided opportunities to demonstrate NTT in locations throughout the country; however, the absence of a clearly defined, consistent approach to parameterization and validation has raised questions over the reliability and consistency of simulated results. In this study: guidelines for parameterization and validation of APEX were developed based on literature review findings and the authors' experience; and a case study was provided to illustrate how the developed guidelines are applied. The developed guidelines are in the form of recommendations covering essential phases of model simulation studies as well as a clear interpretation of model performance evaluation criteria thresholds and model simulation performance results. These guidelines were successfully applied in the central Ohio case study. The most sensitive water yield parameters and their respective reasonable range of values were determined. Simulated monthly and annual water yield values were within 5% and 15% of observed values during the calibration and validation periods, respectively. Overall, the developed guidelines together with the illustrative case study example are intended to serve as the framework to parameterize and validate APEX to support nation-wide deployment of NIT. This framework can be easily modified and used in additional APEX and other modeling studies. Published by Elsevier B.V. C1 [Moriasi, Daniel N.; Teet, Stephen] USDA ARS, Grazinglands Res Lab, 7207 W Cheyenne St, El Reno, OK 73036 USA. [King, Kevin W.; Williams, Mark R.] USDA ARS, Soil Drainage Res Lab, Columbus, OH 43210 USA. [Bosch, David D.] USDA ARS, Southeast Watershed Res, Tifton, GA 31793 USA. [Bjorneberg, Dave L.] USDA ARS, Northwest Irrigat & Soils Res Lab, Kimberly, ID 83341 USA. [Guzman, Jorge A.] Waterborne Environm Inc, Champaign, IL 61820 USA. RP Moriasi, DN (reprint author), USDA ARS, Grazinglands Res Lab, 7207 W Cheyenne St, El Reno, OK 73036 USA. EM daniel.moriasi@ars.usda.gov FU USDA-OEM FX Funding for this study was provided by USDA-OEM. NR 57 TC 0 Z9 0 U1 6 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD NOV PY 2016 VL 177 BP 146 EP 164 DI 10.1016/j.agwat.2016.07.009 PG 19 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DY7PY UT WOS:000385322900014 ER PT J AU Anapalli, SS Ahuja, LR Gowda, PH Ma, LW Marek, G Evett, SR Howell, TA AF Anapalli, Saseendran S. Ahuja, Lajpat R. Gowda, Prasanna H. Ma, Liwang Marek, Gary Evett, Steven R. Howell, Terry A. TI Simulation of crop evapotranspiration and crop coefficients with data in weighing lysimeters SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Evapotranspiration; Crop coefficient; Lysimeter; Agricultural system model; Irrigation; Water management ID CENTRAL GREAT-PLAINS; CLIMATE-CHANGE; MODEL; WATER; IRRIGATION; RZWQM2; AGRICULTURE; ACCURACY; SYSTEM; MAIZE AB Accurate quantification of crop evapotranspiration (ET) is critical to optimizing irrigation water productivity, especially, in the semiarid regions of the world where limited rainfall is supplemented by irrigation for crop production. In this context, cropping system models are potential tools for predicting ET or crop water requirements in agriculture across soils and climates and assist in developing decision support tools for irrigation. The objective of this study was to evaluate the accuracy of RZWQM2 simulated ET for fully irrigated silage (2006 and 2007) and grain corn (1990) against measured crop water use and soil evaporation with large weighing lysimeters in the Texas High Plains. An extended Shuttleworth and Wallace method was used to estimate potential crop ET (E and T) demand in RZWQM2. The Nimah and Hanks approach was used for crop water uptake and Richard's Equation for soil water redistribution modeling. Simulations of biomass, leaf area index, soil water storage, and ET were reasonably close to the measured data. Root Mean Squared Deviation (RMSD) for corn biomass was between 1 and 2.1 MT ha(-1), LAI between 0.33 and 0.88, water in the soil between 2 and 2.9 cm for a 190 cm soil profile, and actual daily crop ET between 1.0 to 1.5 mm across the three years of measured data. Arithmetic mean deviation (MD) for ET ranged from -0.10 to 0.40 mm. Fallow soil evaporation before and after corn planting was simulated within MD of -0.03-0.003 mm. The crop coefficients (Kc) calculated with measured ET and the short grass or alfalfa crop reference ET methods varied from year to year. The Kc values obtained by using the simulated ET and alfalfa reference ET were close to Kc values using measured ET, within RMSD of 0.17, and could be used to obtain long-term average Kc values for scheduling irrigation. Published by Elsevier B.V. C1 [Anapalli, Saseendran S.] USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. [Ahuja, Lajpat R.; Ma, Liwang] USDA ARS, Agr Syst Res Unit, Ft Collins, CO 80522 USA. [Gowda, Prasanna H.] USDA ARS, Forage & Livestock Prod Res Unit, El Reno, OK USA. [Marek, Gary; Evett, Steven R.] USDA ARS, CPRL, 2300 Expt Stn Rd, Bushland, TX 79012 USA. [Howell, Terry A.] USDA ARS, POB 5649, Lago Vista, TX USA. RP Anapalli, SS (reprint author), USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. EM Saseendransa@hotmail.com FU USDA-Agricultural Research Service, Kansas State University; Texas A&M AgriLife Research, Texas A&M AgriLife Extension Service, Texas Tech University; West Texas AM University FX This research was supported in part by the Ogallala Aquifer Program, a consortium between USDA-Agricultural Research Service, Kansas State University, Texas A&M AgriLife Research, Texas A&M AgriLife Extension Service, Texas Tech University, and West Texas A&M University. NR 43 TC 0 Z9 0 U1 19 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD NOV PY 2016 VL 177 BP 274 EP 283 DI 10.1016/j.agwat.2016.08.009 PG 10 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DY7PY UT WOS:000385322900026 ER PT J AU Zhang, BB Feng, G Read, JJ Kong, XB Ouyang, Y Adeli, A Jenkins, JN AF Zhang, Bangbang Feng, Gary Read, John J. Kong, Xiangbin Ouyang, Ying Adeli, Ardeshir Jenkins, Johnie N. TI Simulating soybean productivity under rainfed conditions for major soil types using APEX model in East Central Mississippi SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Simulating; APEX model; Soybean productivity; Rainfed condition; Yield difference; Irrigation management ID CROP PRODUCTION; YIELD GAPS; ZEA-MAYS; WATER; IRRIGATION; MAIZE; MANAGEMENT; DRY; WHEAT; WET AB Knowledge of soybean yield constraints under rainfed conditions on major soil types in East Central Mississippi would assist growers in the region to effectively utilize the benefits of water/irrigation management. The objectives of this study were to use the Agricultural Policy/Environmental eXtender (APEX) agro-ecosystem model to simulate rainfed soybean grain yield (GY) for nine major soils during 14 years (2002-2015) and then to evaluate selected model inputs/outputs in relation to irrigation management that may decrease difference in simulated GY among the different soils. Values for GY ranged broadly from 2.24 to 6.14 Mg ha(-1) across soils and years, giving a maximum yield difference of 3.90 Mg ha(-1). For the average GY of nine soils, the range was from 3.52 to 5.42 Mg ha(-1) over 14 years. Averaged across 14 years, GY ranged from 3.66 to 4.90 Mg ha(-1) across the nine soils and was affected by difference in soil texture (clay and sand percentages) and soil available water content (AWC). Simulations revealed relatively high water stress during the R4, R5 and R6 stages of plant development (early-to mid-fruit development), suggesting great potential to enhance soybean yield if some irrigation is provided during these critical water stress periods; whereas, the potential was accordingly less in the normal and wet years. Results indicated installing irrigation on Griffith, Sumter or Demopolis soils would have a large impact through increased crop productivity and yield stability. (C) 2016 Elsevier B.V. All rights reserved. C1 [Zhang, Bangbang; Kong, Xiangbin] China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China. [Zhang, Bangbang; Kong, Xiangbin] Natl Minist Land & Resources, Lab Farmland Qual Monitoring & Control, Beijing 100193, Peoples R China. [Feng, Gary; Read, John J.; Adeli, Ardeshir; Jenkins, Johnie N.] ARS, Genet & Sustainable Agr Res Unit, USDA, Starkville, MS 39762 USA. [Ouyang, Ying] US Forest Serv, Ctr Bottomland Hardwoods Res, USDA, Starkville, MS 39762 USA. RP Kong, XB (reprint author), China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China. EM kxb@cau.edu.cn FU National Social Science Foundation of China [14AZD031]; Beijing Natural Science Foundation, China [8151001]; Missisippi Soybean Promotion Board [62-2015]; China Scholarship Council [201406350040] FX This work was completed in Genetics and Sustainable Agriculture Research Unit, USDA-ARs as a visiting Ph.D candidate sponsered by China Scholarship Council (No. 201406350040). This work was supported by National Social Science Foundation of China (Grant No. 14AZD031), Beijing Natural Science Foundation, China (Grant No. 8151001), and Missisippi Soybean Promotion Board (62-2015). We especially thank Dr. Luca Doro for his help in APEX model application and Dr. Zhen Sui for her assistance in statistical analysis. NR 54 TC 1 Z9 1 U1 12 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD NOV PY 2016 VL 177 BP 379 EP 391 DI 10.1016/j.agwat.2016.08.022 PG 13 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DY7PY UT WOS:000385322900037 ER PT J AU Han, M Zhang, HH DeJonge, KC Comas, LH Trout, TJ AF Han, Ming Zhang, Huihui DeJonge, Kendall C. Comas, Louise H. Trout, Thomas J. TI Estimating maize water stress by standard deviation of canopy temperature in thermal imagery SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Maize; Water stress; Canopy temperature standard deviation; Thermal imagery; Deficit irrigation ID INFRARED THERMOMETRY; LEAF TEMPERATURES; INDEX; IRRIGATION; INDICATOR; TRANSPIRATION; VARIABILITY; RADIATION; TREES; CORN AB A new crop water stress indicator, standard deviation of canopy temperature within a thermal image (CTSD), was developed to monitor crop water status. In this study, thermal imagery was taken from maize (Zea mays L.) under various levels of deficit irrigation at different crop growing stages. The Expectation Maximization algorithm was used to estimate the canopy temperature distribution from thermal imagery under a range of crop coverage and water stress conditions. Soil water deficit (SWD), leaf water potential (psi), stomatal conductance, and other crop water stress indices were used to evaluate the CTSD. We found that the temperature differences between sunlit and shaded parts of the canopy would increase with larger canopy resistance in the sunlit part of the crop canopy. The CTSD well described impact of irrigation events (timing and depth) on crop water stress. All water stress measurements showed statistically significant relationship with CTSD. Although CTSD is not sensitive to small changes in water stress, the result suggests that the canopy temperature standard deviation could be used as a water stress indicator. This index has strong application potential because it only relies on the canopy temperature itself, and is easy to calculate. Moreover, it may also be applied to high resolution thermal imagery from other remote sensing platforms, such as unmanned aerial vehicles. Published by Elsevier B.V. C1 [Han, Ming; Zhang, Huihui; DeJonge, Kendall C.; Comas, Louise H.; Trout, Thomas J.] USDA ARS, Water Management & Syst Res Unit, 2150 Ctr Ave,Bldg D,Suite 320, Ft Collins, CO 80526 USA. [Han, Ming] Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80526 USA. RP Zhang, HH (reprint author), USDA ARS, Water Management & Syst Res Unit, 2150 Ctr Ave,Bldg D,Suite 320, Ft Collins, CO 80526 USA. EM Huihui.Zhang@ars.usda.gov OI Zhang, Huihui/0000-0002-9781-6086; Trout, Thomas/0000-0003-1896-9170 NR 32 TC 0 Z9 0 U1 14 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD NOV PY 2016 VL 177 BP 400 EP 409 DI 10.1016/j.agwat.2016.08.031 PG 10 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DY7PY UT WOS:000385322900039 ER PT J AU Zhang, BB Feng, G Kong, XB Lal, R Ouyang, Y Adeli, A Jenkins, JN AF Zhang, Bangbang Feng, Gary Kong, Xiangbin Lal, Rattan Ouyang, Ying Adeli, Ardeshir Jenkins, Johnie N. TI Simulating yield potential by irrigation and yield gap of rainfed soybean using APEX model in a humid region SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Soybean; Yield potential; Yield gap; Irrigation timing and amount; Irrigation cost-return analysis; APEX model ID WATER-USE EFFICIENCY; US CORN-BELT; CROP YIELD; DEFICIT IRRIGATION; PLANTING DATE; SEED YIELD; CLAY SOIL; MANAGEMENT; EVAPORATION; GROWTH AB Soybean is generally grown under rainfed conditions in a humid region, Mississippi, USA. In order to determine how much maximum yield could be increased by irrigation, it is crucial to investigate the yield potential (Y-p) without any drought stress and yield gap (Y-g, between Y-p and rainfed yield (Y-w)). Further, it is also important to determine the amount of irrigation water needed to alleviate any drought stress during the entire growing season, and conduct cost-return analysis for irrigated soybean. Therefore, the objectives of this study were to: (1) simulate Y-p, analyze Y-g and determine the irrigation timing and amount needed to achieve Y-p for soybean using the Agricultural Policy/Environmental eXtender (APEX) model; and (2) compute water use efficiency (WUE), irrigation water use efficiency (I-WUE) and conduct the cost-return analysis on irrigation events. Simulated Y-p of soybean without water stress for nine soil types from 2002 to 2014 ranged from 4.47 to 6.51 Mg ha(-1), and was strongly correlated with accumulative solar radiation during the growing season (R-2 = 0.71, P <= 0.01). The Y-g in dry years was much higher than that in normal and wet years, with average Y-g of 1.58, 0.60 and 0.71 Mg ha(-1) for dry, normal and wet years, respectively. Griffith, Sumter and Demopolis soils had the highest average Y-g over 13 years, ranging from 1.37 to 1.60 Mg ha(-1). The average irrigation amount was 308, 192 and 157 mm in dry, normal and wet years, respectively. The average irrigation amount was 75 mm from R1 to R8 stages. The WUE of nine soil types from 2002 to 2014, under non-limiting water conditions, ranged from 9.5 to 13.8 kg ha(-1) mm(-1). The magnitude of Y-g was the principle factor affecting I-WUE among nine soil types. The average I-WUE over 13 years ranged from 1.8 to 7.8 kg ha(-1) mm(-1) for nine soil types. Compared with a rainfed condition, average net return of irrigated soybean increased by 93 $ ha(-1) (dollar per hectare) among nine soil types from 2002 to 2014. The average net return increased by 195, 58 and 70 $ ha(-1) for dry, normal and wet years, respectively. (C) 2016 Elsevier B.V. All rights reserved. C1 [Zhang, Bangbang; Kong, Xiangbin] China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China. [Zhang, Bangbang; Kong, Xiangbin] Natl Minist Land & Resources, Key Lab Farmland Qual Monitoring & Control, Beijing 100193, Peoples R China. [Feng, Gary; Adeli, Ardeshir; Jenkins, Johnie N.] ARS, Genet & Sustainable Agr Res Unit, USDA, Starkville, MS 39762 USA. [Lal, Rattan] Ohio State Univ, Sch Environm & Nat Resources, Carbon Management & Sequestrat Ctr, Columbus, OH 43210 USA. [Ouyang, Ying] US Forest Serv, Ctr Bottomland Hardwoods Res, USDA, Starkville, MS 39762 USA. RP Kong, XB (reprint author), China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China. EM kxb@cau.edu.cn FU Chinese Scholar Council [201406350040]; National Social Science Foundation of China [14AZD031]; Natural Science Foundation of Beijing, China [8151001]; Mississippi Soybean Promotion Board [62-2015] FX This work was completed in Genetics and Sustainable Agriculture Research Unit, USDA-ARS as a visiting Ph.D candidate sponsored by Chinese Scholar Council (No. 201406350040). This work was supported by National Social Science Foundation of China (Grant No. 14AZD031), Natural Science Foundation of Beijing, China (Grant No. 8151001), and Mississippi Soybean Promotion Board (62-2015). We especially thank Luca Doro for his help in APEX model application and Joshua H. Holtcamp for his lab work measuring soil and crop parameters. NR 73 TC 0 Z9 0 U1 13 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD NOV PY 2016 VL 177 BP 440 EP 453 DI 10.1016/j.agwat.2016.08.029 PG 14 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DY7PY UT WOS:000385322900043 ER PT J AU Good, C Marancik, DP Welch, TJ May, T Davidson, J Summerfelt, S AF Good, Christopher Marancik, David P. Welch, Timothy J. May, Travis Davidson, John Summerfelt, Steven TI Systemic granuloma observed in Atlantic salmon Salmo salar raised to market size in a freshwater recirculation aquaculture system SO AQUACULTURE RESEARCH LA English DT Article DE Atlantic salmon; systemic granuloma; histopathology; recirculation aquaculture ID BLOOD-CHEMISTRY; RAINBOW-TROUT; FISH; AGENT C1 [Good, Christopher; May, Travis; Davidson, John; Summerfelt, Steven] Conservat Fund, Inst Freshwater, 1098 Turner Rd, Shepherdstown, WV 25443 USA. [Marancik, David P.; Welch, Timothy J.] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV USA. [Marancik, David P.] Fish Vet Grp, 350 Commercial St, Portland, ME 04101 USA. RP Good, C (reprint author), Conservat Fund, Inst Freshwater, 1098 Turner Rd, Shepherdstown, WV 25443 USA. EM c.good@freshwaterinstitute.org FU Gordon and Betty Moore Foundation; Atlantic Salmon Federation; USDA Agricultural Research Service (ARS) [59-1930-5-510] FX We are grateful to the Gordon and Betty Moore Foundation and the Atlantic Salmon Federation for providing funding for the overall salmon grow-out trial, as well as the USDA Agricultural Research Service (ARS) that supported this independent study under Agreement No. 59-1930-5-510. Our gratitude is extended to Dr Gregory Wiens of the USDA-ARS-NCCCWA for critical review of the manuscript. Appreciation is also extended to Ms Jennifer Lipscomb (USDA-ARS-NCCCWA) for conducting the molecular analyses. The authors do not have any conflicts of interest to declare. Experimental protocols described are in compliance with the Animal Welfare Act (9CFR), and were approved by our Institutional Animal Care and Use Committee (IACUC). Use of trade names does not imply endorsement by the US Government. NR 18 TC 0 Z9 0 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1355-557X EI 1365-2109 J9 AQUAC RES JI Aquac. Res. PD NOV PY 2016 VL 47 IS 11 BP 3679 EP 3683 DI 10.1111/are.12790 PG 5 WC Fisheries SC Fisheries GA DZ1SZ UT WOS:000385621700031 ER PT J AU Lin, WC Li, XJ Dai, ZK Zhang, XH Chang, S Zhao, P Zhang, HM Chen, F Xie, QM AF Lin, Wencheng Li, Xinjian Dai, Zhenkai Zhang, Xinheng Chang, Shuang Zhao, Peng Zhang, Huanmin Chen, Feng Xie, Qingmei TI Molecular epidemiology of J-subgroup avian leukosis virus isolated from meat-type chickens in southern China between 2013 and 2014 SO ARCHIVES OF VIROLOGY LA English DT Article ID POLYMERASE-CHAIN-REACTION; COMPLETE PROVIRAL GENOME; SEQUENCE-ANALYSIS; UNITED-STATES; LAYER FLOCKS; HEMANGIOMA; FIELD; HPRS-103; ELEMENT; STRAIN AB Members of avian leukosis virus subgroup J (ALV-J) cause various diseases associated with tumor formation and decreased fertility, resulting in major economic losses in the poultry industry worldwide. To assess the status of ALV-J infection in meat-type chickens in southern China, the molecular epidemiology of ALV-J strains was investigated. A total of 265 clinical samples collected from southern China from 2013 to 2014 were investigated in this study for the presence of ALV-J, which resulted in 12 virus isolates. Phylogenetic analysis showed that 91.7 % (11/12) of the ALV-J isolates have possessed high homology to Chinese layer isolates and belong to one subgroup. One of the ALV isolates (designated GD1411-1) was relatively closely related to the ALV-J broiler isolates, indicating that the GD1411-1 isolate might be a transition strain. Several unique nucleotide substitutions in gp85 and the U3 region were detected in all 12 ALV-J isolates. This study provides some interesting information on the molecular characterization of ALV-J isolates. These findings will be beneficial for understanding of the pathogenic mechanism of ALV-J infection. C1 [Lin, Wencheng; Li, Xinjian; Dai, Zhenkai; Zhang, Xinheng; Chen, Feng; Xie, Qingmei] South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Key Lab Chicken Genet Breeding & Reprod,Minist Ed, Guangzhou 510642, Guangdong, Peoples R China. [Chang, Shuang; Zhao, Peng] Shandong Agr Univ, Coll Vet Med, Tai An 271018, Shandong, Peoples R China. [Zhang, Huanmin] ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. [Lin, Wencheng; Chen, Feng; Xie, Qingmei] Key Lab Anim Hlth Aquaculture & Environm Control, Guangzhou 510642, Guangdong, Peoples R China. [Lin, Wencheng; Xie, Qingmei] Southe China Collaborat Innovat Ctr Poultry Dis C, Guangzhou 510642, Guangdong, Peoples R China. RP Xie, QM (reprint author), South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Key Lab Chicken Genet Breeding & Reprod,Minist Ed, Guangzhou 510642, Guangdong, Peoples R China.; Xie, QM (reprint author), Key Lab Anim Hlth Aquaculture & Environm Control, Guangzhou 510642, Guangdong, Peoples R China.; Xie, QM (reprint author), Southe China Collaborat Innovat Ctr Poultry Dis C, Guangzhou 510642, Guangdong, Peoples R China. EM qmx@scau.edu.cn FU Natural Science Foundation of Guangdong Province [S2013030013313]; National Natural Science Foundation of China [31472217]; Guangdong Province Science and Technology Plan Project [2012B020306002, 2012B091100078] FX This study was supported by the Natural Science Foundation of Guangdong Province (Grant No. S2013030013313), National Natural Science Foundation of China (Grant No. 31472217) and Guangdong Province Science and Technology Plan Project (Grant Nos. 2012B020306002, 2012B091100078). NR 24 TC 0 Z9 0 U1 2 U2 2 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD NOV PY 2016 VL 161 IS 11 BP 3039 EP 3046 DI 10.1007/s00705-016-3003-8 PG 8 WC Virology SC Virology GA DY6QF UT WOS:000385252100009 PM 27503348 ER PT J AU Seo, EY Lim, S Hammond, J Moon, JS Lim, HS AF Seo, Eun-Young Lim, Seungmo Hammond, John Moon, Jae Sun Lim, Hyoun-Sub TI Complete genome sequence of a novel potyvirus, callistephus mottle virus, identified in Callistephus chinensis SO ARCHIVES OF VIROLOGY LA English DT Article ID GENE AB The complete genomic sequence of a novel putative member of the genus Potyvirus was detected from Callistephus chinensis (China aster) in South Korea. The genomic RNA consists of 9,859 nucleotides (excluding the 3' poly(A) tail) and contains the typical open reading frame of potyviruses, encoding a putative large polyprotein of 3,154 amino acids. The Callistephus virus is most closely related to plum pox virus and members of the ApVY subgroup which showed 50-52 % polyprotein amino acid sequence identity. These results suggest that the Callistephus virus is a novel member of the genus Potyvirus, tentatively named "callistephus mottle virus" (CalMV). C1 [Seo, Eun-Young; Lim, Hyoun-Sub] Chungnam Natl Univ, Dept Appl Biol, Daejeon 305764, South Korea. [Lim, Seungmo; Moon, Jae Sun] Korea Res Inst Biosci & Biotechnol, Mol Biofarming Res Ctr, Daejeon 305806, South Korea. [Lim, Seungmo; Moon, Jae Sun] Univ Sci & Technol, Biosyst & Bioengn Program, Daejeon 305350, South Korea. [Hammond, John] USDA ARS, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA. RP Lim, HS (reprint author), Chungnam Natl Univ, Dept Appl Biol, Daejeon 305764, South Korea.; Moon, JS (reprint author), Korea Res Inst Biosci & Biotechnol, Mol Biofarming Res Ctr, Daejeon 305806, South Korea.; Moon, JS (reprint author), Univ Sci & Technol, Biosyst & Bioengn Program, Daejeon 305350, South Korea. EM jsmoon@kribb.re.kr; hyounlim@cnu.ac.kr FU Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ011113] FX This research was supported by a Grant from the Next-Generation BioGreen 21 Program (PJ011113), Rural Development Administration, Republic of Korea. NR 10 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD NOV PY 2016 VL 161 IS 11 BP 3281 EP 3283 DI 10.1007/s00705-016-3001-x PG 3 WC Virology SC Virology GA DY6QF UT WOS:000385252100034 PM 27480416 ER PT J AU Pan, ZL Johnson, DA Wei, ZJ Ma, L Rong, YP AF Pan, Zhanlei Johnson, Douglas A. Wei, Zhijun Ma, Lei Rong, Yuping TI Non-growing season soil CO2 efflux patterns in five land-use types in northern China SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Grazed steppe; Leymus chinensis pasture; Annual cropland; Carbon dioxide; Inter-annual variability ID CARBON-DIOXIDE FLUXES; INTERANNUAL VARIABILITY; AGROPASTORAL REGION; INNER-MONGOLIA; RESPIRATION; GRASSLANDS; EXCHANGE; ECOSYSTEM; CLIMATE; STEPPE AB Heavy grazing and unsuitable farming practices have led to grassland degradation in northern China. This study examined soil CO2 efflux (Fc) from five land-use types during the non-growing season on the southeastern edge of the Mongolian Plateau in China. The land-use types included three native vegetation steppes subjected to differing stocking rates [ungrazed (UG), moderately grazed (MG) and heavily grazed (HG)], a fertilized annual cropland (CL) and a perennial pasture (PP) used for haying and winter grazing. Values of Fc were measured at 3-day to 2-week intervals during the non-growing season in two contrasting hydrological years (2012-13 and 2013-14) using closed chambers. The Fc during 1 Oct. 2013 to 30 April 2014 averaged 475 mg C m(-2) for all sites compared to a significantly (P < 0.05) lower Fc (102 mg C m(-2)) during 1 Oct. 2012 to 30 April 2013. The seasonal Fc patterns followed the same trend during the two non-growing seasons with greater Fc observed in the autumn and spring freeze-thaw periods compared to the winter permanently frozen period, which accounted for 4.8% of accumulated total non-growing season Fc. The heavily grazed site showed less soil CO2 efflux compared to UG, MG, PP and CL land-use types due to a larger reduction in gross primary productivity (GPP) compared to ecosystem respiration. Grazing reduced Fc by 23% for MG and 32% for HG compared to UG. Soil CO2 efflux from the PP land-use type, which was grazed during the non-growing season, was 23% greater than that from the UG and CL land-use types. Air temperature during the non-growing season was the main factor controlling soil CO2 efflux (R-2 = 0.40, P < 0.001), although soil water content also played a role. Precipitation received during the growing season had a large legacy effect on Fc. Annual weather variation overshadowed the influence of land-use types on Fc. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Pan, Zhanlei; Wei, Zhijun] Inner Mongolia Agr Univ, Coll Ecol & Environm Sci, Huhot 010018, Peoples R China. [Pan, Zhanlei; Ma, Lei; Rong, Yuping] China Agr Univ, Anim Sci & Technol Coll, Beijing Key Lab Grassland Sci, Beijing 100193, Peoples R China. [Johnson, Douglas A.] Utah State Univ, USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA. [Ma, Lei] Chinese Acad Sci, Inst Atmospher Phys, State Key Lab Atmospher Boundary Layer Phys & Atm, Beijing 100029, Peoples R China. RP Rong, YP (reprint author), China Agr Univ, Anim Sci & Technol Coll, Beijing Key Lab Grassland Sci, Beijing 100193, Peoples R China. EM rongyuping@cau.edu.cn FU National Natural Science Foundation of China [31472136]; National Forage Production System Project in China [CARS-35]; National Key Basic Research Program of China [2014CB138805] FX Research was funded by the National Natural Science Foundation of China (31472136), National Forage Production System Project (CARS-35) in China and National Key Basic Research Program of China (2014CB138805). NR 43 TC 0 Z9 0 U1 25 U2 25 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD NOV PY 2016 VL 144 BP 160 EP 167 DI 10.1016/j.atmosenv.2016.08.085 PG 8 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DY7RD UT WOS:000385326100016 ER PT J AU Watkins, BA Kim, J Kenny, A Pedersen, TL Pappan, KL Newman, JW AF Watkins, Bruce A. Kim, Jeffrey Kenny, Anne Pedersen, Theresa L. Pappan, Kirk L. Newman, John W. TI Circulating levels of endocannabinoids and oxylipins altered by dietary lipids in older women are likely associated with previously identified gene targets SO BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS LA English DT Article DE Postmenopausal women; Endocannabinoids; Oxylipins; Global metabolites ID POLYUNSATURATED FATTY-ACIDS; CORONARY-HEART-DISEASE; POSTMENOPAUSAL WOMEN; HUMAN-PLATELETS; OMEGA-3-FATTY-ACID INTAKE; DOCOSAHEXAENOIC ACIDS; FISH-OIL; METABOLISM; SUPPLEMENTATION; METABOLOMICS AB Postmenopausal women (PMW) report marginal n - 3 PUFA intakes and are at risk of chronic diseases associated with the skeletal, muscular, neuroendocrine, and cardiovascular systems. How n - 3 PUFA affect the amounts of endocannabinoids (ECs) and oxylipins (OLs) of metabolic and physiologic importance in PMW is not clear. Based on our recent findings that dietary n - 3 PUFA alter gene targets of the EC system and lower pro-inflammatory OL we proceeded to characterize these actions in blood of PMW. Our aim was to determine levels of the ECs, OLs, and global metabolites (GM) in white PMW (75 +/- 7 y), randomized in a double-masked manner, from baseline to 6 mo after receiving a fish oil supplement of n - 3 PUFA (720 mg 20:5n3 + 480 mg 22:6n3/d, n = 20) or placebo (1.8 g oleic acid/d, n = 20). ECs and OLs in serum were determined by UPLC-MS/MS and GM by GC-MS and LC-MS/MS. Plasma 20:5n3 and 22:6n3 levels increased in PMW given fish oil. EC n - 6 acyl-ethanolamides,arachidonate-derived diols were decreased and 20:5n3 and 22:6n3 diols, epoxides, and alcohols were increased in PMW given fish oil. GM analysis revealed that n - 3 PUFA supplementation increased renal steroid hormone and proteolytic metabolite levels in PMW. Herein, we confirm that gene targets of the EC system, previously found as modifiable by n - 3 PUFA result in changes in the levels of ECs and OLs in PMW. This study shows phenotypic responses (in levels) to n - 3 PUFA supplementation in PMW and increases of n - 3 acyl-ethanolamide and n - 3-derived OL of clinical considerations in aging. (C) 2016 Elsevier B.V. All rights reserved. C1 [Watkins, Bruce A.; Newman, John W.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Watkins, Bruce A.; Kenny, Anne] Univ Connecticut, Ctr Hlth, Ctr Aging, Farmington, CT USA. [Kim, Jeffrey] Univ Calif Davis, Genome & Biomed Sci Facil, Davis, CA 95616 USA. [Pedersen, Theresa L.; Newman, John W.] ARS, USDA, WHNRC, Davis, CA USA. [Pappan, Kirk L.] Metabolon Inc, Durham, NC USA. RP Watkins, BA (reprint author), Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. EM baw@purdue.edu; jefkim@ucdavis.edu; kenny@uchc.edu; Theresa.Pedersen@ARS.USDA.GOV; KPappan@metabolon.com; John.Newman@ARS.USDA.GOV FU University of Connecticut; UCHC, Center on Aging; USDA [5306-51530-019-00D]; West Coast Metabolomics Center [NIH U24 DK097154] FX Watkins organized the research, performed the FAME analyses and did most of the writing; Kenny collected samples and supervised the human study; Kim conducted the analyses of endocannabinoids and oxylipins under the supervision of Pedersen and Newman; Pappan performed the global metabolite analyses and data analysis was performed by Newman, Watkins and Pappan. All authors reviewed and help revise the manuscript. This research was supported by funds to BAW for the metabolomics collaborations at the University of Connecticut and the UCHC, Center on Aging. Additional support was provided by the USDA Intramural Projects 5306-51530-019-00D and the West Coast Metabolomics Center Grant NIH U24 DK097154. The USDA is an equal opportunity provider and employer. NR 57 TC 0 Z9 0 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1388-1981 EI 0006-3002 J9 BBA-MOL CELL BIOL L JI Biochim. Biophys. Acta Mol. Cell Biol. Lipids PD NOV PY 2016 VL 1861 IS 11 BP 1693 EP 1704 DI 10.1016/j.bbalip.2016.07.007 PG 12 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA DY7RA UT WOS:000385325800011 PM 27452639 ER PT J AU Wilhelm, C Harrison, O Schmitt, V Pelletier, M Spencer, S Urban, J Siegel, R Belkaid, Y AF Wilhelm, C. Harrison, O. Schmitt, V. Pelletier, M. Spencer, S. Urban, J. Siegel, R. Belkaid, Y. TI FATTY ACID METABOLISM SUSTAINS ILC2 MEDIATED BARRIER IMMUNITY DURING INFECTION AND MALNUTRITION SO CYTOKINE LA English DT Meeting Abstract CT 4th Annual Meeting of The International-Cytokine-and-Interferon-Society (ICIS) CY OCT 16-19, 2016 CL San Francisco, CA SP Int Cytokine & Interferon Soc C1 [Wilhelm, C.; Schmitt, V.] Univ Bonn, Bonn, Germany. [Harrison, O.; Spencer, S.; Siegel, R.; Belkaid, Y.] NIH, Bldg 10, Bethesda, MD 20892 USA. [Pelletier, M.] Univ Laval, Quebec City, PQ, Canada. [Urban, J.] USDA, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1043-4666 EI 1096-0023 J9 CYTOKINE JI Cytokine PD NOV PY 2016 VL 87 MA 3P214 BP 152 EP 152 PG 1 WC Biochemistry & Molecular Biology; Cell Biology; Immunology SC Biochemistry & Molecular Biology; Cell Biology; Immunology GA DY7PH UT WOS:000385321100247 ER PT J AU Echeverria-Londono, S Newbold, T Hudson, LN Contu, S Hill, SLL Lysenko, I Arbelaez-Cortes, E Armbrecht, I Boekhout, T Cabra-Garcia, J Dominguez-Haydar, Y Nates-Parra, G Gutierrez-Lamus, DL Higuera, D Isaacs-Cubides, PJ Lopez-Quintero, CA Martinez, E Miranda-Esquivel, DR Navarro-Iriarte, LE Noriega, JA Otavo, SE Parra-H, A Poveda, K Ramirez-Pinilla, MP Rey-Velasco, JC Rosselli, L Smith-Pardo, AH Urbina-Cardona, JN Purvis, A AF Echeverria-Londono, Susy Newbold, Tim Hudson, Lawrence N. Contu, Sara Hill, Samantha L. L. Lysenko, Igor Arbelaez-Cortes, Enrique Armbrecht, Inge Boekhout, Teun Cabra-Garcia, Jimmy Dominguez-Haydar, Yamileth Nates-Parra, Guiomar Gutierrez-Lamus, Doris L. Higuera, Diego Johanna Isaacs-Cubides, Paola Lopez-Quintero, Carlos A. Martinez, Eliana Rafael Miranda-Esquivel, Daniel Eduardo Navarro-Iriarte, Luis Ari Noriega, Jorge Eduardo Otavo, Samuel Parra-H, Alejandro Poveda, Katja Ramirez-Pinilla, Martha P. Carlos Rey-Velasco, Juan Rosselli, Loreta Smith-Pardo, Allan H. Nicolas Urbina-Cardona, Jose Purvis, Andy TI Modelling and projecting the response of local assemblage composition to land use change across Colombia SO DIVERSITY AND DISTRIBUTIONS LA English DT Article DE community composition; GLMM; hindcast; land use impact; Neotropics; PREDICTS project; representative concentration pathways ID TROPICAL RAIN-FORESTS; BIOTIC HOMOGENIZATION; SECONDARY FORESTS; BIODIVERSITY LOSS; TERRESTRIAL BIODIVERSITY; GLOBAL BIODIVERSITY; SPECIES RICHNESS; MASS EXTINCTION; HABITAT LOSS; CONSERVATION AB Aim Understanding the impact of land use change within assemblages is fundamental to mitigation policies at local and regional scale. Here, we aim to quantify how site-level terrestrial assemblages are responding to land use change in Colombia a mega-diverse country and to project future biodiversity under different scenarios of land use change associated with climate change policies. Location Colombia (northern South America). Methods We collated original biodiversity data from 17 publications (285 sites) that examined how human impact affects terrestrial biodiversity in Colombia. From each site we estimated compositional intactness (i.e. compositional similarity to undisturbed sites). We fitted generalized linear mixed-effects models to estimate how these measures of local biodiversity vary across land use habitats. Using space-for-time substitution, we applied our estimates to hindcast biodiversity changes since 1500 and project future changes under climate change policies of the four representative concentration pathways (RCPs). Results Assemblages in urban, cropland and pasture sites were compositionally very different from those in primary vegetation. We infer that average compositional intactness has been reduced by 18% across Colombia to date, with strong regional variation. The best RCP scenario for future biodiversity is GCAM-RCP4.5, a path that favours the expansion of secondary forests under a strong carbon market; while the worst is MESSAGE-RCP8.5, 'the business-as-usual' scenario. Main conclusions Land use change has driven an increasing change in the composition of ecological assemblages in Colombia. By 2095, the implementation of carbon markets policy of climate change from GCAM-RCP4.5 could mitigate these changes in community composition. In contrast, the business-as-usual scenario MESSAGE-RCP8.5 predicts a steep community change placing the quality of ecosystems at risk. C1 [Echeverria-Londono, Susy; Hudson, Lawrence N.; Contu, Sara; Hill, Samantha L. L.; Purvis, Andy] Nat Hist Museum, Dept Life Sci, Cromwell Rd, London SW7 5BD, England. [Echeverria-Londono, Susy; Lysenko, Igor; Purvis, Andy] Imperial Coll London, Dept Life Sci, Buckhurst Rd, Ascot SL5 7QN, Berks, England. [Newbold, Tim; Hill, Samantha L. L.] United Nations Environm Programme World Conservat, 219 Huntingdon Rd, Cambridge CB3 0DL, England. [Newbold, Tim] UCL, Dept Genet Evolut & Environm, Ctr Biodivers & Environm Res, Gower St, London WC1E 6BT, England. [Arbelaez-Cortes, Enrique] Inst Invest Recursos Biol Alexander von Humboldt, Colecc Tejidos, Km 17 Carretera Cali Palmira CIAT, Valle Del Cauca, Colombia. [Arbelaez-Cortes, Enrique] Univ Ind Santander, Grp Estudios Biodiversidad, Escuela Biol, Bucaramanga, Colombia. [Armbrecht, Inge] Univ Valle, Dept Biol, Apartado Aereo 25360, Cali, Colombia. [Boekhout, Teun] CBS Fungal Biodivers Ctr CBS KNAW, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands. [Cabra-Garcia, Jimmy] Univ Sao Paulo, Inst Biociencias, Dept Zool, BR-05508090 Sao Paulo, SP, Brazil. [Cabra-Garcia, Jimmy] Univ Valle, Dept Biol, Secc Entomol, Grp Invest Biol Ecol & Manejo Hormigas, Cali 25360, Colombia. [Dominguez-Haydar, Yamileth] Univ Atlantico, Programa Biol, Km 7 Via Puerto Colombia, Atlantico, Colombia. [Nates-Parra, Guiomar] Univ Nacl Cordoba, Dept Biol, Carrera 6 76-103, Monteria, Colombia. [Gutierrez-Lamus, Doris L.] Inst Amazonico Invest Cient Sinchi, Grp Fauna Amazon, Leticia, Colombia. [Higuera, Diego] Corp Sentido Nat, Carrera 70H 122 A-98,Apartamento 101, Bogota, DC, Colombia. [Johanna Isaacs-Cubides, Paola] Pontificia Univ Javeriana, Lab Herpetol, Bogota, DC, Colombia. [Lopez-Quintero, Carlos A.] Univ Antioquia, Inst Biol, Lab TEHO, POB 1226,Calle 67 N 53-108, Medellin, Colombia. [Martinez, Eliana] Univ Nacl Colombia, Dept Biol, Bogota 7945, DC, Colombia. [Rafael Miranda-Esquivel, Daniel; Carlos Rey-Velasco, Juan] Univ Ind Santander, Escuela Biol, Colecc Entomol, Lab Sistemat & Biogeog, Bucaramanga, Colombia. [Eduardo Navarro-Iriarte, Luis] Univ Magdalena, San Andres De Sotavento, Colombia. [Ari Noriega, Jorge] Univ Los Andes, Lab Zool & Ecol Acuat LAZOEA, Bogota, DC, Colombia. [Eduardo Otavo, Samuel] Univ Concepcion, Fac Ciencias Forestales, Lab Ecol Paisaje, Concepcion, Chile. [Parra-H, Alejandro] Corp Gest Serv Ecosistem Polinizac & Abejas SEPyA, Bogota, DC, Colombia. [Parra-H, Alejandro] Univ Nacl Colombia, Dept Biol, Lab Invest Abejas LABUN, Carrera 45 26-85, Bogota, DC, Colombia. [Poveda, Katja] Cornell Univ, Dept Entomol, 4126 Comstock Hall, Ithaca, NY 14853 USA. [Ramirez-Pinilla, Martha P.] Univ Ind Santander, Colecc Herpetol, Bucaramanga, Colombia. [Ramirez-Pinilla, Martha P.] Univ Ind Santander, Grp Estudios Biodiversidad, Escuela Biol, Lab Biol Reprod Vertebrados, Bucaramanga, Colombia. [Carlos Rey-Velasco, Juan] Inst Invest Recursos Biol Alexander von Humboldt, Calle 28 N 15-09, Bogota, DC, Colombia. [Rosselli, Loreta] Univ Ciencias Aplicadas & Ambientales, Fac Ciencias Ambientales, Bogota, DC, Colombia. [Smith-Pardo, Allan H.] USDA, San Francisco, CA USA. [Smith-Pardo, Allan H.] Univ Nacl Colombia, Sede Medellin UNALMED, Fac Ciencias, Medellin, Colombia. [Nicolas Urbina-Cardona, Jose] Pontificia Univ Javeriana, Dept Ecol & Terr, Bogota, DC, Colombia. RP Echeverria-Londono, S (reprint author), Nat Hist Museum, Dept Life Sci, Cromwell Rd, London SW7 5BD, England. EM susye@nhm.ac.uk RI Newbold, Tim/D-1524-2012; Purvis, Andy/A-7529-2008; OI Newbold, Tim/0000-0001-7361-0051; Purvis, Andy/0000-0002-8609-6204; Echeverria-londono, Susy/0000-0002-0038-146X FU UK Natural Environment Research Council (NERC) [NE/J011193/2]; PREDICTS project; Colciencias (Administrative department of Science, Technology and Innovation of Colombia) FX We thank three anonymous referees and the associate editor Piero Visconti for greatly improving the content and framing of this article. We thank Shai Meiri for sharing the reptile species richness information, and the IMAGE, HYDE, MESSAGE and GCAM teams, especially Allison Thomson, for sharing additional data from their integrated assessment models. This study was supported by the UK Natural Environment Research Council (NERC, grant number: NE/J011193/2), who fund the PREDICTS project (http://www.predicts.org.uk/), and Colciencias (Administrative department of Science, Technology and Innovation of Colombia) who fund S.E.-L.'s PhD programme. PREDICTS is endorsed by the GEO BON. This is a contribution from the Imperial College Grand Challenges in Ecosystems and the Environment Initiative. NR 77 TC 1 Z9 1 U1 15 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1366-9516 EI 1472-4642 J9 DIVERS DISTRIB JI Divers. Distrib. PD NOV PY 2016 VL 22 IS 11 BP 1099 EP 1111 DI 10.1111/ddi.12478 PG 13 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DZ0TJ UT WOS:000385552300003 ER PT J AU Dile, YT Daggupati, P George, C Srinivasan, R Arnold, J AF Dile, Yihun T. Daggupati, Prasad George, Chris Srinivasan, Raghavan Arnold, Jeff TI Introducing a new open source GIS user interface for the SWAT model SO ENVIRONMENTAL MODELLING & SOFTWARE LA English DT Article DE Open source software; QGIS; QSWAT; SWAT; Gumera watershed; Ethiopia ID DOWNSTREAM ECOSYSTEM SERVICES; MESSAGE-PASSING INTERFACE; WATER-QUALITY; UNCERTAINTY; FLOW; OPPORTUNITIES; CALIBRATION AB The Soil and Water Assessment Tool (SWAT) model is a robust watershed modeling tool. It typically uses the ArcSWAT interface to create its inputs. ArcSWAT is public domain software which works in the licensed ArcGIS environment. The aim of this paper was to develop an open source user interface for the SWAT model. The interface, QSWAT, is written in the Python programming language and uses various functionalities of the open source geographic information system, QGIS. The current interface performs similar functions to ArcSWAT, but with additional enhanced features such as merging small subbasins and static and dynamic visualization of outputs. The interface is demonstrated through a case study in the Gumera watershed in the Lake Tana basin of Ethiopia, where it showed a successful performance. QSWAT will be a valuable tool for the SWAT scientific community, with improved availability and functionality compared with other options for creating SWAT models. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Dile, Yihun T.; Srinivasan, Raghavan] Texas A&M Univ, Spatial Sci Lab, Ecosyst Sci & Management Dept, College Stn, TX 77801 USA. [Daggupati, Prasad] Univ Guelph, Sch Engn, Guelph, ON, Canada. [George, Chris] United Nations Univ, Int Inst Software Technol, Macau, Peoples R China. [Arnold, Jeff] USDA ARS, Grassland Soil & Water Res Lab, 808 East Blackland Rd, Temple, TX 76502 USA. RP Dile, YT (reprint author), Texas A&M Univ, Spatial Sci Lab, Ecosyst Sci & Management Dept, College Stn, TX 77801 USA. EM yihundile@tamu.edu RI Daggupati, Prasad/D-8886-2017; OI Daggupati, Prasad/0000-0002-7044-3435; Srinivasan, Raghavan/0000-0001-8375-6038; Dile, Yihun/0000-0002-3419-2018 FU Feed the Future through the U.S. Agency for International Development [AID-OAA-A-13-0005] FX This publication was partly made possible through support provided by Feed the Future through the U.S. Agency for International Development, under the terms of Contract No.AID-OAA-A-13-0005. The opinions expressed herein are those of the authors and do not necessarily reflect the views of the U.S. Agency for International Development. We thank the Ethiopian National Meteorological Services Agency and the Ethiopian Ministry of Water, Energy, and Irrigation for providing us with observed weather and streamflow data, respectively. Comments from three anonymous reviewers truly helped to improve the manuscript. We appreciate them. NR 44 TC 0 Z9 0 U1 37 U2 37 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1364-8152 EI 1873-6726 J9 ENVIRON MODELL SOFTW JI Environ. Modell. Softw. PD NOV PY 2016 VL 85 BP 129 EP 138 DI 10.1016/j.envsoft.2016.08.004 PG 10 WC Computer Science, Interdisciplinary Applications; Engineering, Environmental; Environmental Sciences SC Computer Science; Engineering; Environmental Sciences & Ecology GA DZ1JX UT WOS:000385595800010 ER PT J AU Confalonieri, R Bregaglio, S Adam, M Ruget, F Li, T Hasegawa, T Yin, XY Zhu, Y Boote, K Buis, S Fumoto, T Gaydon, D Lafarge, T Marcaida, M Nakagawa, H Ruane, AC Singh, B Singh, U Tang, L Tao, FL Fugice, J Yoshida, H Zhang, Z Wilson, LT Baker, J Yang, YB Masutomi, Y Wallach, D Acutis, M Bouman, B AF Confalonieri, Roberto Bregaglio, Simone Adam, Myriam Ruget, Francoise Li, Tao Hasegawa, Toshihiro Yin, Xinyou Zhu, Yan Boote, Kenneth Buis, Samuel Fumoto, Tamon Gaydon, Donald Lafarge, Tanguy Marcaida, Manuel Nakagawa, Hiroshi Ruane, Alex C. Singh, Balwinder Singh, Upendra Tang, Liang Tao, Fulu Fugice, Job Yoshida, Hiroe Zhang, Zhao Wilson, Lloyd T. Baker, Jeff Yang, Yubin Masutomi, Yuji Wallach, Daniel Acutis, Marco Bouman, Bas TI A taxonomy-based approach to shed light on the babel of mathematical models for rice simulation SO ENVIRONMENTAL MODELLING & SOFTWARE LA English DT Article DE Model classification; Model parameterisation; Model ensemble; Model structure; Rice; Uncertainty ID CROP MODELS; SENSITIVITY-ANALYSIS; CLIMATE-CHANGE; CALIBRATION; YIELD; WHEAT; UNCERTAINTY; WATER; PLASTICITY; EVOLUTION AB For most biophysical domains, differences in model structures are seldom quantified. Here, we used a taxonomy-based approach to characterise thirteen rice models. Classification keys and binary attributes for each key were identified, and models were categorised into five clusters using a binary similarity measure and the unweighted pair-group method with arithmetic mean. Principal component analysis was performed on model outputs at four sites. Results indicated that (i) differences in structure often resulted in similar predictions and (ii) similar structures can lead to large differences in model outputs. User subjectivity during calibration may have hidden expected relationships between model structure and behaviour. This explanation, if confirmed, highlights the need for shared protocols to reduce the degrees of freedom during calibration, and to limit, in turn, the risk that user subjectivity influences model performance. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Confalonieri, Roberto; Bregaglio, Simone; Acutis, Marco] Univ Milan, Cassandra Lab, I-20122 Milan, Italy. [Adam, Myriam; Lafarge, Tanguy] CIRAD, UMR AGAP, F-34398 Montpellier, France. [Ruget, Francoise; Buis, Samuel] INRA, EMMAH UMR1114, F-84914 Avignon, France. [Li, Tao; Marcaida, Manuel; Bouman, Bas] Int Rice Res Inst, Los Banos, Philippines. [Hasegawa, Toshihiro; Fumoto, Tamon] Natl Inst Agroenvironm Sci, Tsukuba, Ibaraki, Japan. [Yin, Xinyou] Wageningen Univ, Ctr Crop Syst Anal, NL-6700 AP Wageningen, Netherlands. [Zhu, Yan; Tang, Liang] Nanjing Agr Univ, Natl Engn & Technol Ctr Informat Agr, Jiangsu Key Lab Informat Agr, Jiangsu Collaborat Innovat Ctr Modern Crop Prod, Nanjing, Jiangsu, Peoples R China. [Boote, Kenneth] Univ Florida, Gainesville, FL USA. [Gaydon, Donald] CSIRO Agr & Food, Brisbane, Qld, Australia. [Nakagawa, Hiroshi; Yoshida, Hiroe] Natl Agr & Food Res Org, Tsukuba, Ibaraki, Japan. [Ruane, Alex C.] NASA, Goddard Inst Space Studies, New York, NY USA. [Singh, Upendra; Fugice, Job] Int Fertilizer Dev Ctr, Muscle Shoals, AL 35662 USA. [Tao, Fulu] Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100864, Peoples R China. [Zhang, Zhao] Beijing Normal Univ, State Key Lab Earth Surface Proc & Resource Ecol, Beijing 100875, Peoples R China. [Wilson, Lloyd T.; Yang, Yubin] Texas A&M AgriLife Res & Extens Ctr, Beaumont, TX USA. [Baker, Jeff] ARS, USDA, Big Spring, TX USA. [Masutomi, Yuji] Ibaraki Univ, Coll Agr, Mito, Ibaraki, Japan. [Wallach, Daniel] INRA, UMR1248, Agrosyst & Dev Terr, F-31326 Castanet Tolosan, France. [Singh, Balwinder] CIMMYT, CG Block,NASC Complex, New Delhi 110012, India. [Tao, Fulu] Nat Resources Inst Finland Luke, Vantaa 01301, Finland. [Li, Tao] AgMIP Rice Team, Tsukuba, Ibaraki, Japan. RP Confalonieri, R (reprint author), Univ Milan, Cassandra Lab, I-20122 Milan, Italy. EM roberto.confalonieri@unimi.it RI Gaydon, Donald /F-4608-2012; OI Wallach, Daniel/0000-0003-3500-8179; Hasegawa, Toshihiro/0000-0001-8501-5612; , Balwinder-Singh/0000-0002-6715-2207 FU National Science Foundation of China [31561143003]; Academy of Finland, PLUMES project [277403]; MAFF; Global Environment Research of MOE, Japan [S-10-2]; National High Tech Research and Development Program of China [2013AA100404]; EC-FP7 [613817] FX FT is supported by the National Science Foundation of China (31561143003) and the Academy of Finland, PLUMES project (277403). TH's participation was supported by MAFF and the Global Environment Research (S-10-2) of MOE, Japan. YZ's work was supported by the National High Tech Research and Development Program of China (2013AA100404). RC was partly supported by EC-FP7 under Grant Agreement No. 613817 (MODEXTREME). Nanjing Agricultural University, International Rice Research Institute, Ministry of Agriculture, Forestry and Fisheries and Ministry of the Environment of Japan, and Cassandra lab. of the University of Milan supported the annual research planning meetings of the AgMIP Rice Team. USDA is an equal opportunity provider and employer. NR 56 TC 0 Z9 0 U1 4 U2 4 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1364-8152 EI 1873-6726 J9 ENVIRON MODELL SOFTW JI Environ. Modell. Softw. PD NOV PY 2016 VL 85 BP 332 EP 341 DI 10.1016/j.envsoft.2016.09.007 PG 10 WC Computer Science, Interdisciplinary Applications; Engineering, Environmental; Environmental Sciences SC Computer Science; Engineering; Environmental Sciences & Ecology GA DZ1JX UT WOS:000385595800024 ER PT J AU Bello, NM Kramer, M Tempelman, RJ Stroup, WW St-Pierre, NR Craig, BA Young, LJ Gbur, EE AF Bello, Nora M. Kramer, Matthew Tempelman, Robert J. Stroup, Walter W. St-Pierre, Normand R. Craig, Bruce A. Young, Linda J. Gbur, Edward E. TI Short communication: On recognizing the proper experimental unit in animal studies in the dairy sciences SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE experimental unit; replication; observational unit; hierarchical data structure; pen ID POLICY AB Sound design of experiments combined with proper implementation of appropriate statistical methods for data analysis are critical for producing meaningful scientific results that are both replicable and reproducible. This communication addresses specific aspects of design and analysis of experiments relevant to the dairy sciences and, in so doing, responds to recent concerns raised in a letter to the editor of the Journal of Dairy Science regarding journal policy for research publications on pen-based animal studies. We further elaborate on points raised, rectify interpretation of important concepts, and show how aspects of statistical inference and elicitation of research conclusions are affected. C1 [Bello, Nora M.] Kansas State Univ, Dept Stat, Manhattan, KS 66506 USA. [Kramer, Matthew] ARS, USDA, Beltsville, MD 20705 USA. [Tempelman, Robert J.] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. [Stroup, Walter W.] Univ Nebraska, Dept Stat, Lincoln, NE 68583 USA. [St-Pierre, Normand R.] Ohio State Univ, Dept Anim Sci, Columbus, OH 43210 USA. [Craig, Bruce A.] Purdue Univ, Dept Stat, W Lafayette, IN 47907 USA. [Young, Linda J.] Natl Agr Stat Serv, Washington, DC 20250 USA. [Gbur, Edward E.] Univ Arkansas, Agr Stat Lab, Fayetteville, AR 72701 USA. RP Bello, NM (reprint author), Kansas State Univ, Dept Stat, Manhattan, KS 66506 USA. EM nbello@ksu.edu RI Craig, Bruce/D-5797-2017 OI Craig, Bruce/0000-0001-9346-467X NR 16 TC 1 Z9 1 U1 10 U2 10 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD NOV PY 2016 VL 99 IS 11 BP 8871 EP 8879 DI 10.3168/jds.2016-11516 PG 9 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DZ1PQ UT WOS:000385610700034 PM 27614832 ER PT J AU Krueger, LA Beitz, DC Humphrey, SB Stabel, JR AF Krueger, L. A. Beitz, D. C. Humphrey, S. B. Stabel, J. R. TI Gamma delta T cells are early responders to Mycobacterium avium ssp paratuberculosis in colostrum-replete Holstein calves SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE dairy calf; colostrum; vitamins; cellular immunity ID SUBSP PARATUBERCULOSIS; IMMUNE-RESPONSE; DAIRY CALVES; MILK; INFECTION; GROWTH; HAPTOGLOBIN; LACTATION; INDUCTION; REPLACER AB Peripheral blood mononuclear cells (PBMC) and mesenteric node lymphocytes (MNL) were obtained from 30 calves that were assigned randomly at birth to 1 of 6 treatment groups with 5 calves per treatment in a 14-d study: (1) colostrum-deprived (CD), no vitamins; (2) colostrum-replacer (CR), no vitamins; (3) CR, vitamin A; (4) CR, vitamin D-3; (5) CR, vitamin E; (6) CR, vitamins A, D-3, E. Calves were injected with appropriate vitamin supplements and fed pasteurized whole milk (CD calves) or fractionated colostrum-replacer (CR calves) at birth. Thereafter, all calves were fed pasteurized whole milk fortified with vitamins according to treatment group. Calves were orally inoculated with 10(8) cfu of Mycobacterium avium ssp. paratuberculosis (MAP) on d 1 and 3. The PBMC and MNL harvested on d 13 were analyzed by flow cytometry as fresh cells, after 3-d culture with phytohemagglutinin (PHA), and after 6-d culture with a whole-cell sonicate of MAP (MPS). Peripheral gamma delta T cells were a predominant lymphocyte subset in neonatal calves, with a decreased percentage noted in CD calves compared with CR calves. As well, CD25 expression was higher in gamma delta T cells compared with other cell subsets, regardless of treatment group. Stimulation of PBMC with PHA resulted in increased CD4(+) and CD8(+) subsets, whereas MNL response was dominated by expansion of B-cell subpopulations. Stimulation with PHA and MPS decreased the relative abundance of PBMC gamma delta T cells, but MNL gamma delta T cells increased upon stimulation with MPS. These results identify gamma delta T cells as key early responders to intracellular infection in neonatal calves and suggest that colostrum may be an important mediator of this response. C1 [Krueger, L. A.; Beitz, D. C.; Stabel, J. R.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. [Krueger, L. A.; Stabel, J. R.] ARS, Infect Bacterial Dis Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Humphrey, S. B.] ARS, Microscopy Serv, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. RP Stabel, JR (reprint author), Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA.; Stabel, JR (reprint author), ARS, Infect Bacterial Dis Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. EM judy.stabel@ars.usda.gov NR 25 TC 0 Z9 0 U1 2 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD NOV PY 2016 VL 99 IS 11 BP 9040 EP 9050 DI 10.3168/jds.2016-11144 PG 11 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DZ1PQ UT WOS:000385610700049 PM 27614838 ER PT J AU Niederwerder, MC Nietfeld, JC Bai, JF Peddireddi, L Breazeale, B Anderson, J Kerrigan, MA An, BY Oberst, RD Crawford, K Lager, KM Madson, DM Rowland, RRR Anderson, GA Hesse, RA AF Niederwerder, Megan C. Nietfeld, Jerome C. Bai, Jianfa Peddireddi, Lalitha Breazeale, Barbara Anderson, Joe Kerrigan, Maureen A. An, Baoyan Oberst, Richard D. Crawford, Kimberly Lager, Kelly M. Madson, Darin M. Rowland, Raymond R. R. Anderson, Gary A. Hesse, Richard A. TI Tissue localization, shedding, virus carriage, antibody response, and aerosol transmission of Porcine epidemic diarrhea virus following inoculation of 4-week-old feeder pigs SO JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION LA English DT Article DE Coronavirus; diarrhea; Porcine epidemic diarrhea virus; swine; transmission ID UNITED-STATES; INFECTION AB We determined tissue localization, shedding patterns, virus carriage, antibody response, and aerosol transmission of Porcine epidemic diarrhea virus (PEDV) following inoculation of 4-week-old feeder pigs. Thirty-three pigs were randomly assigned to 1 of 3 groups for the 42-day study: inoculated (group A; n = 23), contact transmission (group B; n = 5), and aerosol transmission (group C; n = 5). Contact transmission occurred rapidly to group B pigs whereas productive aerosol transmission failed to occur to group C pigs. Emesis was the first clinical sign noted at 3 days postinoculation (dpi) followed by mild to moderate diarrhea lasting 5 more days. Real-time PCR detected PEDV in fecal and nasal swabs, oral fluids, serum, and gastrointestinal and lymphoid tissues. Shedding occurred primarily during the first 2 weeks postinoculation, peaking at 5-6 dpi; however, some pigs had PEDV nucleic acid detected in swabs collected at 21 and 28 dpi. Antibody titers were measurable between 14 and 42 dpi. Although feces and intestines collected at 42 dpi were PEDV negative by PCR and immunohistochemistry, respectively, small intestines from 70% of group A pigs were PCR positive. Although disease was relatively mild and transient in this age group, the results demonstrate that 4-week-old pigs are productively infected and can sustain virus replication for several weeks. Long-term shedding of PEDV in subclinically affected pigs should be considered an important source for PEDV transmission. C1 [Niederwerder, Megan C.; Nietfeld, Jerome C.; Bai, Jianfa; Peddireddi, Lalitha; Kerrigan, Maureen A.; Oberst, Richard D.; Rowland, Raymond R. R.; Anderson, Gary A.; Hesse, Richard A.] Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, 1800 Denison Ave, Manhattan, KS 66506 USA. [Niederwerder, Megan C.; Nietfeld, Jerome C.; Bai, Jianfa; Peddireddi, Lalitha; Breazeale, Barbara; Anderson, Joe; An, Baoyan; Oberst, Richard D.; Anderson, Gary A.; Hesse, Richard A.] Kansas State Univ, Coll Vet Med, Kansas State Vet Diagnost Lab, 1800 Denison Ave, Manhattan, KS 66506 USA. [Crawford, Kimberly; Lager, Kelly M.] USDA ARS, Natl Anim Dis Ctr, POB 70, Ames, IA 50010 USA. [Madson, Darin M.] Iowa State Univ, Dept Vet Diagnost & Prod Anim Med, Ames, IA USA. RP Niederwerder, MC (reprint author), Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, 1800 Denison Ave, Manhattan, KS 66506 USA.; Niederwerder, MC (reprint author), Kansas State Univ, Coll Vet Med, Kansas State Vet Diagnost Lab, 1800 Denison Ave, Manhattan, KS 66506 USA. EM mniederwerder@vet.k-state.edu FU National Pork Board [13-228] FX The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by the National Pork Board award number 13-228. NR 13 TC 0 Z9 0 U1 6 U2 6 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1040-6387 EI 1943-4936 J9 J VET DIAGN INVEST JI J. Vet. Diagn. Invest. PD NOV PY 2016 VL 28 IS 6 BP 671 EP 678 DI 10.1177/1040638716663251 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA DZ4CM UT WOS:000385805400009 PM 27698163 ER PT J AU Pantin-Jackwood, MJ Costa-Hurtado, M Shepherd, E DeJesus, E Smith, D Spackman, E Kapczynski, DR Suarez, DL Stallknecht, DE Swayne, DE AF Pantin-Jackwood, Mary J. Costa-Hurtado, Mar Shepherd, Eric DeJesus, Eric Smith, Diane Spackman, Erica Kapczynski, Darrell R. Suarez, David L. Stallknecht, David E. Swayne, David E. TI Pathogenicity and Transmission of H5 and H7 Highly Pathogenic Avian Influenza Viruses in Mallards SO JOURNAL OF VIROLOGY LA English DT Article ID DUCKS ANAS-PLATYRHYNCHOS; WILD BIRDS; EXPERIMENTAL-INFECTION; MIGRATORY BIRDS; DOMESTIC DUCKS; A VIRUSES; NATURAL INFECTION; POULTRY OUTBREAKS; CAIRINA-MOSCHATA; MUSCOVY DUCKS AB Wild aquatic birds have been associated with the intercontinental spread of H5 subtype highly pathogenic avian influenza (HPAI) viruses of the A/goose/Guangdong/1/96 (Gs/GD) lineage during 2005, 2010, and 2014, but dispersion by wild waterfowl has not been implicated with spread of other HPAI viruses. To better understand why Gs/GD H5 HPAI viruses infect and transmit more efficiently in waterfowl than other HPAI viruses, groups of mallard ducks were challenged with one of 14 different H5 and H7 HPAI viruses, including a Gs/GD lineage H5N1 (clade 2.2) virus from Mongolia, part of the 2005 dispersion, and the H5N8 and H5N2 index HPAI viruses (clade 2.3.4.4) from the United States, part of the 2014 dispersion. All virus-inoculated ducks and contact exposed ducks became infected and shed moderate to high titers of the viruses, with the exception that mallards were resistant to Ck/Pennsylvania/83 and Ck/Queretaro/95 H5N2 HPAI virus infection. Clinical signs were only observed in ducks challenged with the H5N1 2005 virus, which all died, and with the H5N8 and H5N2 2014 viruses, which had decreased weight gain and fever. These three viruses were also shed in higher titers by the ducks, which could facilitate virus transmission and spread. This study highlights the possible role of wild waterfowl in the spread of HPAI viruses. C1 [Pantin-Jackwood, Mary J.; Costa-Hurtado, Mar; Shepherd, Eric; DeJesus, Eric; Smith, Diane; Spackman, Erica; Kapczynski, Darrell R.; Suarez, David L.; Swayne, David E.] ARS, Exot & Emerging Avian Viral Dis Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr,USDA, Athens, GA 30605 USA. [Stallknecht, David E.] Univ Georgia, Southeastern Cooperat Wildlife Dis Study, Athens, GA 30602 USA. [Costa-Hurtado, Mar] Univ Montreal, Fac Vet Med, Res Grp Infect Dis Prod Anim, St Hyacinthe, PQ, Canada. [Costa-Hurtado, Mar] Univ Montreal, Fac Vet Med, Swine & Poultry Infect Dis Res Ctr, St Hyacinthe, PQ, Canada. [Spackman, Erica] Univ Georgia, Coll Vet Med, Poultry Diagnost & Res Ctr, Athens, GA USA. RP Pantin-Jackwood, MJ (reprint author), ARS, Exot & Emerging Avian Viral Dis Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr,USDA, Athens, GA 30605 USA. EM Mary.Pantin-Jackwood@ars.usda.gov RI Costa-Hurtado, Mar/R-1496-2016 OI Costa-Hurtado, Mar/0000-0003-4194-0253 FU USDA/ARS CRIS project [6612-32000-063-00D]; Center for Research on Influenza Pathogenesis (CRIP); NIAID [HHSN272201400008C] FX This research was supported by USDA/ARS CRIS project 6612-32000-063-00D and by the Center for Research on Influenza Pathogenesis (CRIP) and the NIAID-funded Center of Excellence in Influenza Research and Surveillance (CEIRS; contract HHSN272201400008C). NR 77 TC 3 Z9 3 U1 13 U2 13 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD NOV PY 2016 VL 90 IS 21 BP 9967 EP 9982 DI 10.1128/JVI.01165-16 PG 16 WC Virology SC Virology GA DZ0JW UT WOS:000385525700035 PM 27558429 ER PT J AU Asztalos, IB Gleason, JA Sever, S Gedik, R Asztalos, BF Horvath, KV Dansinger, ML Lamon-Fava, S Schaefer, EJ AF Asztalos, Ivor B. Gleason, Joi A. Sever, Sakine Gedik, Reyhan Asztalos, Bela F. Horvath, Katalin V. Dansinger, Michael L. Lamon-Fava, Stefania Schaefer, Ernst J. TI Effects of eicosapentaenoic acid and docosahexaenoic acid on cardiovascular disease risk factors: a randomized clinical trial SO METABOLISM-CLINICAL AND EXPERIMENTAL LA English DT Article DE Omega-3 fatty acids; Fish oils; Triglycerides; Lp-PLA(2) ID CORONARY-ARTERY-DISEASE; N-3 FATTY-ACIDS; PHOSPHOLIPASE A(2); HEART-DISEASE; HYPERCHOLESTEROLEMIC PATIENTS; PRIMARY PREVENTION; ICOSAPENT ETHYL; RICH OIL; LIPOPROTEIN; PLASMA AB Background. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), the primary omega-3 fatty acids in fish oil, have been shown to reduce cardiovascular disease (CVD) risk. Objective. This study aimed to examine the independent effects of EPA and DHA on lipid and apolipoprotein levels, as well as on inflammatory biomarkers of CVD risk, using doses often used in the general population. Design. A blinded, randomized 6-week trial was performed in 121 healthy, normolipidemic subjects who received olive oil placebo 6 g/d, EPA 600 mg/d, EPA 1800 mg/d, or DHA 600 mg/d. The EPA was derived from genetically modified yeast. Results. The subjects tolerated the supplements well with no safety issues; and the expected treatment-specific increases in plasma EPA and DHA levels were observed. Compared to placebo, the DHA group had significant decreases in postprandial triglyceride (TG) concentrations (-20%, -52.2 mg/dL, P = 0.03), significant increases in fasting and postprandial low-density lipoprotein cholesterol (LDL-C) (+18.4%, 17.1 mg/dL, P = 0.001), with no significant changes in inflammatory biomarkers. No significant effects were observed in the EPA 600 mg/d group. The high-dose EPA group had significant decreases in lipoprotein-associated phospholipase A2 concentrations (Lp-PLA(2)) (-14.1%, -21.4 ng/mL, P = 0.003). Conclusions. The beneficial effects of EPA 1800 mg/d on CVD risk reduction may relate in part to the lowering of Lp-PLA(2) withbut adversely affecting LDL-C. In contrast, DHA decreased postprandial TG, but raised LDL-C. Our observations indicate that these dietary fatty acids have divergent effects on cardiovascular risk markers. (C) 2016 Published by Elsevier Inc. C1 [Asztalos, Ivor B.; Gleason, Joi A.; Sever, Sakine; Gedik, Reyhan; Dansinger, Michael L.; Schaefer, Ernst J.] Cardiovasc Res Associates, Boston, MA USA. [Asztalos, Ivor B.; Gleason, Joi A.; Asztalos, Bela F.; Horvath, Katalin V.; Dansinger, Michael L.; Lamon-Fava, Stefania; Schaefer, Ernst J.] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer USDA, Cardiovasc Nutr Lab, 711 Washington St, Boston, MA 02111 USA. [Dansinger, Michael L.] Tufts Med Ctr, Div Endocrinol & Metab, Boston, MA USA. [Asztalos, Ivor B.] Childrens Hosp Philadelphia, Philadelphia, PA 19104 USA. RP Schaefer, EJ (reprint author), Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer USDA, Cardiovasc Nutr Lab, 711 Washington St, Boston, MA 02111 USA. EM emst.schaefer@tufts.edu FU NHLBI NIH HHS [P50 HL083813] NR 48 TC 0 Z9 0 U1 9 U2 9 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0026-0495 EI 1532-8600 J9 METABOLISM JI Metab.-Clin. Exp. PD NOV PY 2016 VL 65 IS 11 BP 1636 EP 1645 DI 10.1016/j.metabol.2016.07.010 PG 10 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DZ6TB UT WOS:000385993700007 PM 27733252 ER PT J AU Shine, MB Yang, JW El-Habbak, M Nagyabhyru, P Fu, DQ Navarre, D Ghabrial, S Kachroo, P Kachroo, A AF Shine, M. B. Yang, Jung-Wook El-Habbak, Mohamed Nagyabhyru, Padmaja Fu, Da-Qi Navarre, Duroy Ghabrial, Said Kachroo, Pradeep Kachroo, Aardra TI Cooperative functioning between phenylalanine ammonia lyase and isochorismate synthase activities contributes to salicylic acid biosynthesis in soybean SO NEW PHYTOLOGIST LA English DT Article DE isochorismate synthase (ICS); pathogen defense; phenylalanine ammonia lyase (PAL); salicylic acid (SA); soybean (Glycine max) ID SYSTEMIC ACQUIRED-RESISTANCE; RACE-SPECIFIC RESISTANCE; SYRINGAE PV GLYCINEA; PSEUDOMONAS-SYRINGAE; PHYTOPHTHORA RESISTANCE; PERONOSPORA-PARASITICA; AVIRULENCE GENE; PLANT DEFENSE; BENZOIC-ACID; AMINO-ACID AB Salicylic acid (SA), an essential regulator of plant defense, is derived from chorismate via either the phenylalanine ammonia lyase (PAL) or the isochorismate synthase (ICS) catalyzed steps. The ICS pathway is thought to be the primary contributor of defense-related SA, at least in Arabidopsis. We investigated the relative contributions of PAL and ICS to defense-related SA accumulation in soybean (Glycine max). Soybean plants silenced for five PAL isoforms or two ICS isoforms were analyzed for SA concentrations and SA-derived defense responses to the hemibiotrophic pathogens Pseudomonas syringae and Phytophthora sojae. We show that, unlike in Arabidopsis, PAL and ICS pathways are equally important for pathogen-induced SA biosynthesis in soybean. Knock-down of either pathway shuts down SA biosynthesis and abrogates pathogen resistance. Moreover, unlike in Arabidopsis, pathogen infection is associated with the suppression of ICS gene expression. Pathogen-induced biosynthesis of SA via the PAL pathway correlates inversely with phenylalanine concentrations. Although infections with either virulent or avirulent strains of the pathogens increase SA concentrations, resistance protein-mediated response to avirulent P.sojae strains may function in an SA-independent manner. These results show that PAL- and ICS-catalyzed reactions function cooperatively in soybean defense and highlight the importance of PAL in pathogen-induced SA biosynthesis. C1 [Shine, M. B.; Yang, Jung-Wook; El-Habbak, Mohamed; Nagyabhyru, Padmaja; Fu, Da-Qi; Ghabrial, Said; Kachroo, Pradeep; Kachroo, Aardra] Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA. [Navarre, Duroy] Washington State Univ, Dept Plant Pathol, USDA, ARS, Prosser, WA 99350 USA. RP Kachroo, A (reprint author), Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA. EM apkach2@uky.edu FU Kentucky Soybean Promotion Board; Kentucky Agricultural Experiment Station [16-12-049] FX We thank Adam Bogdanove and Massimo Delledone (providing Psg strains), Scott Abney (providing P.sojae isolates), Todd Pfeiffer (for help with generating the soybean seed stock) and Amy Crume (for management of the plant growth facilities) for their generous help. This work was supported by grants from the Kentucky Soybean Promotion Board. The information reported in this article (no. 16-12-049) is part of a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. NR 55 TC 0 Z9 0 U1 21 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD NOV PY 2016 VL 212 IS 3 BP 627 EP 636 DI 10.1111/nph.14078 PG 10 WC Plant Sciences SC Plant Sciences GA DZ4AE UT WOS:000385797800012 PM 27411159 ER PT J AU Matsubayashi, M Minoura, C Kimura, S Tani, H Furuya, M Lillehoj, HS Matsuda, H Takenaka, S Hatta, T Tsuji, N Sasai, K AF Matsubayashi, Makoto Minoura, Chisa Kimura, Shintaro Tani, Hiroyuki Furuya, Masaru Lillehoj, Hyun S. Matsuda, Haruo Takenaka, Shigeo Hatta, Takeshi Tsuji, Naotoshi Sasai, Kazumi TI Identification of Eimeria acervulina conoid antigen using chicken monoclonal antibody SO PARASITOLOGY RESEARCH LA English DT Article DE Chicken monoclonal antibody; Conoid; Eimeria acervulina; Elongation factor-1 alpha ID HOST-CELL INVASION; CRYPTOSPORIDIUM-PARVUM; APICAL ORGANELLES; IN-VITRO; SPOROZOITES; PROTEIN; TRANSHYDROGENASE; CYTOSKELETON; APICOMPLEXA; TOXOPLASMA AB In the poultry industry, Eimeria spp. is one of the important pathogens which cause significant economic losses. We have previously generated a chicken monoclonal antibody (mAb), 6D-12-G10, with specificity for an antigen located in the apical cytoskeleton of Eimeria acervulina and with cross-reactive among Apicomplexan parasites, including other Eimeria spp., Toxoplasma, Neospora, and Cryptosporidium spp. Furthermore, the protein of Cryptosporidium parvum recognized by the 6D-12-G10 has been identified as elongation factor-1 alpha (EF-1 alpha). In the present study, to identify the target molecule of E. acervulina by the mAb, we performed two-dimensional Western blotting analysis. Finally, we found two positive molecules which are identified as EF-1 alpha and a related protein. Our previous finding using C. parvum and the results in this study suggest that EF-1 alpha could be associated with the invasion facilitated by the cytoskeleton at the apical region of zoites. C1 [Matsubayashi, Makoto; Minoura, Chisa; Kimura, Shintaro; Tani, Hiroyuki; Furuya, Masaru; Takenaka, Shigeo; Sasai, Kazumi] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Div Vet Sci, 1-58 Rinku Orai Kita, Izumisano, Osaka 5988531, Japan. [Lillehoj, Hyun S.] ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD 20705 USA. [Matsuda, Haruo] Hiroshima Univ, Grad Sch Biosphere Sci, Dept Mol & Appl Biosci, Immunobiol Lab, 1-4-4 Kagamiyama, Hiroshima 7398528, Japan. [Hatta, Takeshi; Tsuji, Naotoshi] Kitasato Univ, Dept Parasitol, Sch Med, Minami Ku, 1-15-1 Kitasato, Sagamihara, Kanagawa 2520374, Japan. RP Sasai, K (reprint author), Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Div Vet Sci, 1-58 Rinku Orai Kita, Izumisano, Osaka 5988531, Japan. EM ksasai@vet.osakafu-u.ac.jp FU Ministry of Education, Culture, Sports, and Science [23580445, 25450436] FX We are grateful to Dr. Y. Fukuta, from the APRO Life Science Institute, Inc., for the LC-MS/MS analyses. This study was supported in part by the Grants-in-aid for Scientific Research 23580445 (to K. S., H. T., M. F., and M. M.) and 25450436 (to M. M.) from the Ministry of Education, Culture, Sports, and Science. NR 25 TC 0 Z9 0 U1 3 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 EI 1432-1955 J9 PARASITOL RES JI Parasitol. Res. PD NOV PY 2016 VL 115 IS 11 BP 4123 EP 4128 DI 10.1007/s00436-016-5185-0 PG 6 WC Parasitology SC Parasitology GA DY5LU UT WOS:000385142100006 PM 27384177 ER PT J AU Vemmer, M Schumann, M Beitzen-Heineke, W French, BW Vidal, S Patel, AV AF Vemmer, Marina Schumann, Mario Beitzen-Heineke, Wilhelm French, Bryan W. Vidal, Stefan Patel, Anant V. TI Development of a CO2-releasing coformulation based on starch, Saccharomyces cerevisiae and Beauveria bassiana attractive towards western corn rootworm larvae SO PEST MANAGEMENT SCIENCE LA English DT Article DE carbon dioxide; encapsulation; alginate; attract-and-kill; Diabrotica virgifera virgifera ID WIREWORMS COLEOPTERA-ELATERIDAE; CARBON-DIOXIDE; HOST LOCATION; FEEDING STIMULANTS; CHRYSOMELIDAE LARVAE; BEHAVIORAL-RESPONSES; INOCULANT CARRIERS; ETHANOL-PRODUCTION; KILL STRATEGY; PLANT-GROWTH AB BACKGROUNDCO(2) is known as an attractant for many soil-dwelling pests. To implement an attract-and-kill strategy for soil pest control, CO2-emitting formulations need to be developed. The aim of the present work was to develop a slow-release bead system in order to bridge the gap between application and hatching of western corn rootworm larvae. RESULTSWe compared different Ca-alginate beads containing Saccharomyces cerevisiae for their potential to release CO2 over a period of several weeks. The addition of starch improved CO2 release, resulting in significantly higher CO2 concentrations in soil for at least 4 weeks. The missing amylase activity was compensated for either by microorganisms present in the soil or by coencapsulation of Beauveria bassiana. Formulations containing S. cerevisiae, starch and B. bassiana were attractive for western corn rootworm larvae within the first 4 h following exposure; however, when considering the whole testing period, the maize root systems remained more attractive for the larvae. CONCLUSIONCoencapsulation of S. cerevisiae, starch and B. bassiana is a promising approach for the development of attractive formulations for soil applications. For biological control strategies, the attractiveness needs to be increased by phagostimuli to extend contact between larvae and the entomopathogenic fungus growing out of these formulations. (c) 2016 Society of Chemical Industry C1 [Vemmer, Marina; Patel, Anant V.] Univ Appl Sci, Fac Engn & Math, Fermentat & Formulat Biol & Chem, Interakt 1, D-33619 Bielefeld, Germany. [Schumann, Mario; Vidal, Stefan] Univ Gottingen, Dept Crop Sci, Agr Entomol, Gottingen, Germany. [Beitzen-Heineke, Wilhelm] BIOCARE Gesell Biol Schutzmittel mbH, Einbeck, Germany. [French, Bryan W.] ARS, North Cent Agr Res Lab, USDA, Brookings, SD USA. RP Patel, AV (reprint author), Univ Appl Sci, Fac Engn & Math, Fermentat & Formulat Biol & Chem, Interakt 1, D-33619 Bielefeld, Germany. EM anant.patel@fh-bielefeld.de NR 58 TC 2 Z9 2 U1 13 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD NOV PY 2016 VL 72 IS 11 BP 2136 EP 2145 DI 10.1002/ps.4245 PG 10 WC Agronomy; Entomology SC Agriculture; Entomology GA DZ2IK UT WOS:000385665300015 PM 26834068 ER PT J AU Fayette, J Raid, R Roberts, PD Jones, JB Pernezny, K Bull, CT Goss, EM AF Fayette, Joubert Raid, Richard Roberts, Pamela D. Jones, Jeffiey B. Pernezny, Ken Bull, Carolee T. Goss, Erica M. TI Multilocus Sequence Typing of Strains of Bacterial Spot of Lettuce Collected in the United States SO PHYTOPATHOLOGY LA English DT Article ID CAMPESTRIS PV.-VITIANS; PATHOGEN PSEUDOMONAS-SYRINGAE; LEAF-SPOT; PLANT PATHOGEN; STATISTICAL TESTS; GENETIC DIVERSITY; DNA POLYMORPHISM; 1ST REPORT; XANTHOMONAS; RECOMBINATION AB Studies on genetic diversity and recombination in bacterial pathogens are providing a better understanding of the mechanisms shaping bacterial diversity, which can affect disease control. Xanthomonas campestris pv. vitians, causal agent of bacterial leaf spot of lettuce, is a threat to the worldwide lettuce industry. We examined the genetic variation within a sample of 83 strains from California, Florida, and Ohio using multilocus sequence typing of six housekeeping genes, totaling 2.7 kb. Additionally, polymorphism in two virulence-related genes, hrpB2 and a putative glycosyl hydrolase, were examined. Based on housekeeping genes, we found three genetic groups of strains that were all able to induce the disease. These included strains collected from weeds and irrigation water that had haplotypes identical to strains from diseased lettuce. High linkage disequilibrium across the sequenced loci indicates that the pathogen is predominantly clonal but recombination has contributed to the observed sequence variation. Although there was significant genetic variation in X. campestris pv. vitians within and among sampled states, identical haplotypes were observed across all three states. This finding suggests that seedborne inoculum may contribute to the diversity of X. campestris pv. vitians in the United States. Knowledge of the genetic structure of the pathogen may be used for developing resistant lettuce varieties. C1 [Fayette, Joubert; Raid, Richard; Pernezny, Ken] Univ Florida, Everglades Res & Educ Ctr, Inst Food & Agr Sci, Belle Glade, FL 33430 USA. [Roberts, Pamela D.] Univ Florida, IFAS, Southwest Florida Res & Educ Ctr, Immokalee, FL USA. [Fayette, Joubert; Raid, Richard; Roberts, Pamela D.; Jones, Jeffiey B.; Pernezny, Ken; Goss, Erica M.] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA. [Bull, Carolee T.] USDA ARS, Pacific West Area, 1636 E Alisal St, Salinas, CA USA. [Goss, Erica M.] Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32611 USA. [Bull, Carolee T.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. RP Goss, EM (reprint author), Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA.; Goss, EM (reprint author), Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32611 USA. EM emgoss@ufl.edu FU University of Florida Institute of Food and Agricultural Sciences Everglades Research and Education Center's Emeritus Faculty Research Endowment FX We thank J. Barak and S. Miller for kindly providing the strains from California and Ohio and anonymous reviewers whose comments improved the manuscript. This research was supported by the University of Florida Institute of Food and Agricultural Sciences Everglades Research and Education Center's Emeritus Faculty Research Endowment. NR 53 TC 0 Z9 0 U1 2 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD NOV PY 2016 VL 106 IS 11 BP 1262 EP 1269 DI 10.1094/PHYTO-11-15-0302-R PG 8 WC Plant Sciences SC Plant Sciences GA DZ5KI UT WOS:000385900600003 PM 27359264 ER PT J AU Choudhury, RA Koike, ST Fox, AD Anchieta, A Subbarao, KV Klosterman, SJ McRoberts, N AF Choudhury, R. A. Koike, S. T. Fox, A. D. Anchieta, A. Subbarao, K. V. Klosterman, S. J. McRoberts, N. TI Season-Long Dynamics of Spinach Downy Mildew Determined by Spore Trapping and Disease Incidence SO PHYTOPATHOLOGY LA English DT Article ID PSEUDOPERONOSPORA-CUBENSIS SPORANGIA; BOTRYTIS LEAF-BLIGHT; PERONOSPORA-EFFUSA; AIRBORNE CONIDIA; ERYSIPHE-NECATOR; BREMIA-LACTUCAE; PLANT-DISEASE; REAL-TIME; INFORMATION; PATHOGENS AB Peronospora effusa is an obligate oomycete that causes downy mildew of spinach. Downy mildew threatens sustainable production of fresh market organic spinach in California, and routine fungicide sprays are often necessary for conventional production. In this study, airborne P. effusa spores were collected using rotating arm impaction spore trap samplers at four sites in the Salinas Valley between late January and early June in 2013 and 2014. Levels of P effusa DNA were determined by a species-specific quantitative polymerase chain reaction assay. Peronospora effusa was detected prior to and during the growing season in both years. Nonlinear time series analyses on the data suggested that the within-season dynamics of P. effusa airborne inoculum are characterized by a mixture of chaotic, deterministic, and stochastic features, with successive data points somewhat predictable from the previous values in the series. Analyses of concentrations of airborne P. effusa suggest both an exponential increase in concentration over the course of the season and oscillations around the increasing average value that had season-specific periodicity around 30, 45, and 75 days, values that are close to whole multiples of the combined pathogen latent and infectious periods. Each unit increase in temperature was correlated with 1.7 to 6% increased odds of an increase in DNA copy numbers, while each unit decrease in wind speed was correlated with 4 to 12.7% increased odds of an increase in DNA copy numbers. Disease incidence was correlated with airborne P. effusa levels and weather variables, and a receiver operating characteristic curve analysis suggested that P. effusa DNA copy numbers determined from the spore traps nine days prior to disease rating could predict disease incidence. C1 [Choudhury, R. A.; Subbarao, K. V.; McRoberts, N.] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Koike, S. T.] Univ Calif Cooperat Extens, 1432 Abbott St, Salinas, CA 93901 USA. [Fox, A. D.] Fox Weather LLC, Fortuna, CA 95540 USA. [Anchieta, A.; Klosterman, S. J.] USDA ARS, 1636 E Alisal St, Salinas, CA 93905 USA. RP McRoberts, N (reprint author), Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA.; Klosterman, SJ (reprint author), USDA ARS, 1636 E Alisal St, Salinas, CA 93905 USA. EM steve.klosterman@ars.usda.gov; nmcroberts@ucdavis.edu OI Subbarao, Krishna/0000-0002-2075-1835 FU California Leafy Greens Research Program (CLGRP); California Department of Agriculture Specialty Crop block grant program [SCB14043] FX We thank the California Leafy Greens Research Program (CLGRP) and the California Department of Agriculture Specialty Crop block grant program (number SCB14043) for funding this research. We thank W. Mahaffee (USDA-ARS, Corvallis, OR) for providing some spore trap materials and advice and L. Ochoa (USDA-ARS, Salinas, CA) and R. Pena (Hartnell College, Salinas, CA) for collecting spore trap samples. We also thank two anonymous reviewers for their helpful suggestions and advice. NR 44 TC 0 Z9 0 U1 7 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD NOV PY 2016 VL 106 IS 11 BP 1311 EP 1318 DI 10.1094/PHYTO-12-15-0333-R PG 8 WC Plant Sciences SC Plant Sciences GA DZ5KI UT WOS:000385900600009 PM 27442537 ER PT J AU Wang, YW Lu, HJ Hu, JG AF Wang, Yunwen Lu, Huangjun Hu, Jinguo TI Molecular Mapping of High Resistance to Bacterial Leaf Spot in Lettuce PI 358001-1 SO PHYTOPATHOLOGY LA English DT Article ID LACTUCA-SATIVA L.; GENETIC-LINKAGE MAP; DISEASE RESISTANCE; DOWNY MILDEW; HIGH-DENSITY; SSR MARKERS; CULTIVARS; ASSOCIATION; INHERITANCE; GERMPLASM AB Lettuce (Lactuca sativa L.) is a diploid (2n = 18) with a genome size of 2,600 Mbp, and belongs to the family Compositae. Bacterial leaf spot (BLS), caused by Xanthomonas campestris pv. vitians, is a major disease of lettuce worldwide. Leaf lettuce PI 358001-1 has been characterized as an accession highly resistant to BLS and has white seed. In order to understand inheritance of the high resistance in this germplasm line, an F-3 population consisting of 163 families was developed from the cross PI 358001-1 x 'Tall Guzmaine' (a susceptible Romaine lettuce variety with black seed). The segregation ratio of reaction to disease by seedling inoculation with X. campestris pv. vitians L7 strain in the F-3 families was shown to be 32:82:48 homozygous resistant/heterozygous/homozygous susceptible, fitting to 1:2:1 (n = 162, chi(2) = 3.19, P = 0.20). The segregation ratio of seed color by checking F-2 plants was 122:41 black/white, fitting to 3:1 (n = 163, chi(2) = 0.002, P = 0.96). The results indicated that both BLS resistance and seed color were inherited as a dominant gene mode. A genetic linkage map based on 124 randomly selected F-2 plants was developed to enable molecular mapping of the BLS resistance and the seed color trait. In total, 199 markers, comprising 176 amplified fragment length polymorphisms, 16 simple-sequence repeats, 5 resistant gene candidate markers, and 2 cleaved amplified polymorphic sequences (CAPS) markers were assigned to six linkage groups. The dominant resistance gene to BLS (Xcvr) was mapped on linkage group 2 and the gene locus y for seed color was identified on linkage group 5. Due to the nature of a single gene inheritance, the high-resistance gene should be readily transferred to adapted lettuce cultivars to battle against the devastating disease of lettuce. C1 [Wang, Yunwen; Lu, Huangjun] Univ Florida, Everglades Res & Educ Ctr, Inst Food & Agr Sci, Belle Glade, FL 33430 USA. [Hu, Jinguo] Washington State Univ, USDA ARS, Western Reg Plant Intro Stn, Pullman, WA 99164 USA. RP Wang, YW; Lu, HJ (reprint author), Univ Florida, Everglades Res & Educ Ctr, Inst Food & Agr Sci, Belle Glade, FL 33430 USA. EM ywwang@ufl.edu; hjlu@ufl.edu FU Florida Specialty Crop Block Grant Program; USDA [00114165]; CRIS [FLA-BGL-005105] FX We thank P. McCord, M. Kanaan, and K. McCorkle at the United States Department of Agriculture (USDA) Agricultural Research Service U.S. Sugar Cane Field Station for providing facilities for DNA quality measurement and sample loading; J. Todd for computer software assistance; and A. Carroll, Ph.D. student R. Kandel, and internship undergraduate student G. Faroutine for lab and greenhouse assistance. This work was supported by grants from Florida Specialty Crop Block Grant Program, USDA grant number 00114165, and the CRIS project number FLA-BGL-005105 to H. Lu. NR 42 TC 0 Z9 0 U1 5 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD NOV PY 2016 VL 106 IS 11 BP 1319 EP 1325 DI 10.1094/PHYTO-09-15-0238-R PG 7 WC Plant Sciences SC Plant Sciences GA DZ5KI UT WOS:000385900600010 PM 27454703 ER PT J AU Klos, KE Gordon, T Bregitzer, P Hayes, P Chen, XM del Blanco, IA Fisk, S Bonman, JM AF Klos, K. Esvelt Gordon, T. Bregitzer, P. Hayes, P. Chen, X. M. del Blanco, I. A. Fisk, S. Bonman, J. M. TI Barley Stripe Rust Resistance QTL: Development and Validation of SNP Markers for Resistance to Puccinia striiformis f. sp hordei SO PHYTOPATHOLOGY LA English DT Article ID NORTH-AMERICA; REGISTRATION; DISEASE; GENES; INHERITANCE; POPULATION; VIRULENCE; STRESS; WHEAT; BURST AB Quantitative trait loci (QTL) for barley stripe rust resistance were mapped in recombinant inbred lines (RIL) from a 'Lenetah' x 'Grannelose Zweizeilige' (GZ) cross. GZ is known for a major seedling resistance QTL on chromosome 4H but linked markers suitable for marker-assisted selection have not been developed. This study identified the 4H QTL (log of the likelihood [LOD] = 15.94 at 97.19 centimorgans [cM]), and additional QTL on chromosomes 4H and 6H (LOD = 5.39 at 72.7 cM and 4.24 at 34.46 cM, respectively). A QTL on chromosome 7H (LOD = 2.04 at 81.07 cM) was suggested. All resistance alleles were derived from GZ. Evaluations of adult plant response in Corvallis, OR in 2013 and 2015 provided evidence of QTL at the same positions. However, the minor QTL on 4H was not statistically significant in either location/year, while the 7H QTL was significant in both. The single-nucleotide polymorphism markers flanking the resistance QTL were validated in RIL from a '95SR316A' x GZ cross for their ability to predict seedling resistance. In 95SR316A x GZ, 91 to 92% of RIL with GZ alleles at the major 4H QTL and at least one other were resistant to moderate in reaction. In these populations, at least two QTL were required to transfer the barley stripe rust resistance from GZ. C1 [Klos, K. Esvelt; Gordon, T.; Bregitzer, P.; Bonman, J. M.] ARS, USDA, Aberdeen, ID 83210 USA. [Hayes, P.; Fisk, S.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Chen, X. M.] USDA ARS, Pullman, WA 99164 USA. [del Blanco, I. A.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. RP Klos, KE (reprint author), ARS, USDA, Aberdeen, ID 83210 USA. EM Kathy.klos@ars.usda.gov FU United States Department of Agriculture Agricultural Research Service [2050-21000-031-00] FX This work was supported by the United States Department of Agriculture Agricultural Research Service, Project Number 2050-21000-031-00. We thank R. Campbell and I. Shakelford for their technical assistance and J. Dubcovsky for his contribution to planning for field evaluations. NR 38 TC 0 Z9 0 U1 18 U2 18 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD NOV PY 2016 VL 106 IS 11 BP 1344 EP 1351 DI 10.1094/PHYTO-09-15-0225-R PG 8 WC Plant Sciences SC Plant Sciences GA DZ5KI UT WOS:000385900600013 ER PT J AU Nirmala, J Chao, S Olivera, P Babiker, EM Abeyo, B Tadesse, Z Imtiaz, M Talbert, L Blake, NK Akhunov, E Pumphrey, MO Jin, Y Rouse, MN AF Nirmala, Jayaveeramuthu Chao, Shiaoman Olivera, Pablo Babiker, Ebrahiem M. Abeyo, Bekele Tadesse, Zerihun Imtiaz, Muhammad Talbert, Luther Blake, Nancy K. Akhunov, Eduard Pumphrey, Michael O. Jin, Yue Rouse, Matthew N. TI Markers Linked to Wheat Stem Rust Resistance Gene Sr11 Effective to Puccinia graminis f. sp tritici Race TKTTF SO PHYTOPATHOLOGY LA English DT Article ID VIRULENCE; UG99; GERMPLASM; ETHIOPIA; SPREAD; TTKS AB Wheat stem rust, caused by Puccinia graminis f. sp. tritici, can cause severe yield losses on susceptible wheat varieties and cultivars. Although stem rust can be controlled by the use of genetic resistance, population dynamics of P. graminis f. sp. tritici can frequently lead to defeat of wheat stem rust resistance genes. P. graminis f. sp. tritici race TKTTF caused a severe epidemic in Ethiopia on Ug99-resistant 'Digalu' in 2013 and 2014. The gene Sr11 confers resistance to race TKTTF and is present in 'Gabo 56'. We identified seven single-nucleotide polymorphism (SNP) markers linked to Sr11 from a cross between Gabo 56 and 'Chinese Spring' exploiting a 90K Infinium iSelect Custom beadchip. Five SNP markers were validated on a 'Berkut/'Scalavatis' population that segregated for Sill, using KBioscience competitive allele-specific polymerase chain reaction (KASP) assays. Two of the SNP markers, KASP_6BL_IWB10724 and ICASP_6BL_IWB72471, were predictive of Sr11 among wheat genetic stocks, cultivars, and breeding lines from North America, Ethiopia, and Pakistan. These markers can be utilized to select for Sr11 in wheat breeding and to detect the presence of Sr11 in uncharacterized germplasm. C1 [Nirmala, Jayaveeramuthu; Jin, Yue; Rouse, Matthew N.] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Chao, Shiaoman] USDA ARS, Cereal Crops Res, Fargo, ND 58102 USA. [Olivera, Pablo; Jin, Yue; Rouse, Matthew N.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Babiker, Ebrahiem M.] USDA ARS, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. [Abeyo, Bekele] Int Maize & Wheat Improvement Ctr CIMMYT Ethiopia, Addis Ababa, Ethiopia. [Tadesse, Zerihun] Kulumsa Agr Res Ctr, Ethiopian Inst Agr Res, Kulumsa, Ethiopia. [Imtiaz, Muhammad] CIMMYT Pakistan, Islamabad, Pakistan. [Talbert, Luther; Blake, Nancy K.] Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. [Akhunov, Eduard] Kansas State Univ, Dept Plant Pathol, Throckmorton Hall, Manhattan, KS 66506 USA. [Pumphrey, Michael O.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. RP Rouse, MN (reprint author), USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA.; Rouse, MN (reprint author), Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. EM matthew.rouse@ars.usda.gov FU USDA-ARS Appropriated Project [5062-21220-021-00]; USDA-ARS National Plant Disease Recovery System; USDA-ARS Pakistan Wheat Production Enhancement Program; USAID Feed the Future; Durable Rust Resistance in Wheat project; National Research Initiative Competitive Grant (Triticeae-CAP) from the USDA National Institute of Food and Agriculture [2011-68002-30029] FX Funding for this research was provided by USDA-ARS Appropriated Project 5062-21220-021-00, USDA-ARS National Plant Disease Recovery System, USDA-ARS Pakistan Wheat Production Enhancement Program, USAID Feed the Future, the Durable Rust Resistance in Wheat project, and the National Research Initiative Competitive Grant 2011-68002-30029 (Triticeae-CAP) from the USDA National Institute of Food and Agriculture. We thank the University of Minnesota Supercomputing Institute for computational support. Mention of a trademark, vendor, or proprietary product does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other product or vendors that also may be suitable. NR 36 TC 0 Z9 0 U1 10 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD NOV PY 2016 VL 106 IS 11 BP 1352 EP 1358 DI 10.1094/PHYTO-04-16-0165-R PG 7 WC Plant Sciences SC Plant Sciences GA DZ5KI UT WOS:000385900600014 PM 27359266 ER PT J AU Knaus, BJ Tabima, JF Davis, CE Judelson, HS Grunwald, NJ AF Knaus, B. J. Tabima, J. F. Davis, C. E. Judelson, H. S. Grunwald, N. J. TI Genomic Analyses of Dominant US Clonal Lineages of Phytophthora infestans Reveals a Shared Common Ancestry for Clonal Lineages US11 and US18 and a Lack of Recently Shared Ancestry Among All Other US Lineages SO PHYTOPATHOLOGY LA English DT Article ID IRISH POTATO FAMINE; EASTERN UNITED-STATES; DNA-SEQUENCING DATA; OAK DEATH PATHOGEN; POPULATION-STRUCTURE; CENTRAL MEXICO; PHYLOGENETIC ANALYSIS; SEXUAL REPRODUCTION; GENETIC DIVERSITY; PLANT PATHOGEN AB Populations of the potato and tomato late-blight pathogen Phytophthora infestans are well known for emerging as novel clonal lineages. These successions of dominant clones have historically been named US1 through US24, in order of appearance, since their first characterization using molecular markers. Hypothetically, these lineages can emerge through divergence from other U.S. lineages, recombination among lineages, or as novel, independent lineages originating outside the United States. We tested for the presence of phylogenetic relationships among U.S. lineages using a population of 31 whole-genome sequences, including dominant U.S. clonal lineages as well as available samples from global populations. We analyzed ancestry of the whole mitochondrial genome and samples of nuclear loci, including supercontigs 1.1 and 1.5 as well as several previously characterized coding regions. We found support for a shared ancestry among lineages US11 and US18 from the mitochondrial genome as well as from one nuclear haplotype on each supercontig analyzed. The other nuclear haplotype from each sample assorted independently, indicating an independent ancestry. We found no support for emergence of any other of the U.S. lineages from a common ancestor shared with the other U.S. lineages. Each of the U.S. clonal lineages fit a model where populations of new clonal lineages emerge via migration from a source population that is sexual in nature and potentially located in central Mexico or elsewhere. This work provides novel insights into patterns of emergence of clonal lineages in plant pathogen genomes. C1 [Knaus, B. J.; Grunwald, N. J.] USDA ARS, Hort Crop Res Unit, Corvallis, OR 97330 USA. [Tabima, J. F.; Grunwald, N. J.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Davis, C. E.; Judelson, H. S.] Univ Calif Riverside, Dept Plant Pathol, Riverside, CA 92521 USA. RP Grunwald, NJ (reprint author), USDA ARS, Hort Crop Res Unit, Corvallis, OR 97330 USA.; Grunwald, NJ (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM Nik.Grunwald@ars.usda.gov OI Grunwald, Niklaus/0000-0003-1656-7602 FU United States Department of Agriculture (USDA) Agricultural Research Service Grant [5358-22000-039-00D]; USDA National Institute of Food and Agriculture Grant [2011-68004-30154] FX We thank three anonymous reviewers and the senior editor for many great suggestions that significantly improved this manuscript; J. Ristaino and B. Fry for sharing previously characterized isolates of U.S. clonal lineages; K. Fairchild, M. Larsen, and C. Press for maintenance of the cultures and general technical support; and the Center for Genome Research and Biocomputing at Oregon State University for access to their high-performance computing facility, genome sequencing, and bioinformatic support. This research is supported, in part, by United States Department of Agriculture (USDA) Agricultural Research Service Grant 5358-22000-039-00D and USDA National Institute of Food and Agriculture Grant 2011-68004-30154 (to N. J. Grunwald and H. S. Judelson). NR 78 TC 1 Z9 1 U1 8 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD NOV PY 2016 VL 106 IS 11 BP 1393 EP 1403 DI 10.1094/PHYTO-10-15-0279-R PG 11 WC Plant Sciences SC Plant Sciences GA DZ5KI UT WOS:000385900600019 PM 27348344 ER PT J AU Kunjeti, SG Anchieta, A Martin, FN Choi, YJ Thines, M Michelmore, RW Koike, ST Tsuchida, C Mahaffee, W Subbarao, KV Klosteiman, SJ AF Kunjeti, Sridhara G. Anchieta, Amy Martin, Frank N. Choi, Young-Joon Thines, Marco Michelmore, Richard W. Koike, Steven T. Tsuchida, Cayla Mahaffee, Walt Subbarao, Krishna V. Klosteiman, Steven J. TI Detection and Quantification of Bremia lactucae by Spore Trapping and Quantitative PCR SO PHYTOPATHOLOGY LA English DT Article ID POLYMERASE-CHAIN-REACTION; LETTUCE DOWNY MILDEW; BOTRYTIS LEAF-BLIGHT; MOLECULAR-DETECTION; AIRBORNE INOCULUM; PHYLOGENETIC-RELATIONSHIPS; PYRENOPEZIZA-BRASSICAE; OILSEED RAPE; PATHOGENS; POPULATIONS AB Bremia lactucae is an obligate, oomycete pathogen of lettuce that causes leaf chlorosis and necrosis and adversely affects marketability. The disease has been managed with a combination of host resistance and fungicide applications with success over the years. Fungicide applications are routinely made under the assumption that inoculum is always present during favorable environmental conditions. This approach often leads to fungicide resistance in B. lactucae populations. Detection and quantification of airborne B. lactucae near lettuce crops provides an estimation of the inoculum load, enabling more judicious timing of fungicide applications. We developed a quantitative polymerase chain reaction (qPCR)-based assay using a target sequence in mitochondrial DNA for specific detection of B. lactucae. Validation using amplicon sequencing of DNA from 83 geographically diverse isolates, representing 14 Bremia spp., confirmed that the primers developed for the TaqMan assays are species specific and only amplify templates from B. lactucae. DNA from a single sporangium could be detected at a quantification cycle (C-q) value of 32, and C-q values >35 were considered to be nonspecific. The coefficient of determination (R-2) for regression between sporangial density derived from flow cytometry and Cq values derived from the qPCR was 0.86. The assay was deployed using spore traps in the Salinas Valley, where nearly half of U.S. lettuce is produced. The deployment of this sensitive B. lactucae-specific assay resulted in the detection of the pathogen during the 2-week lettuce-free period as well as during the cropping season. These results demonstrate that this assay will be useful for quantifying inoculum load in and around the lettuce fields for the purpose of timing fungicide applications based on inoculum load. C1 [Kunjeti, Sridhara G.; Subbarao, Krishna V.] Univ Calif Davis, Dept Plant Pathol, 1636 E Alisal St, Salinas, CA 93901 USA. [Anchieta, Amy; Martin, Frank N.; Klosteiman, Steven J.] ARS, USDA, 1636 E Alisal St, Salinas, CA 93905 USA. [Choi, Young-Joon] Kunsan Natl Univ, Dept Biol, Gunsan 54150, South Korea. [Choi, Young-Joon; Thines, Marco] Senckenberg Gesellscharft Nat Forsch, Biodivers & Climate Res Ctr BiK F, D-60325 Frankfurt, Germany. [Choi, Young-Joon; Thines, Marco] Goethe Univ Frankfurt, Dept Biol Sci, Inst Ecol Evolut & Divers, D-60325 Frankfurt, Germany. [Michelmore, Richard W.; Tsuchida, Cayla] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA. [Michelmore, Richard W.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Koike, Steven T.] Univ Calif Cooperat Extens, Salinas, CA USA. [Tsuchida, Cayla] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Mahaffee, Walt] ARS, USDA, Corvallis, OR USA. RP Klosteiman, SJ (reprint author), ARS, USDA, 1636 E Alisal St, Salinas, CA 93905 USA. EM Steve.Klosterman@ars.usda.gov OI Subbarao, Krishna/0000-0002-2075-1835 FU California Leafy Greens Research Program; California Department of Food and Agriculture [SCB14043] FX We acknowledge funding from the California Leafy Greens Research Program and the California Department of Food and Agriculture (grant SCB14043). We thank B. McLaughlin and J. E. Van Dyke for expertise and guidance in sorting sporangia using the BD influx instrument at the University of California-Davis Flow Cytometry Shared Resources; R. Pena, L. Ochoa, and F. Wong (USDA-ARS, Salinas, CA) for collecting spore trap samples; A. I. Putman for insightful review of the manuscript; and B. Smith for help in data analysis. The mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture (USDA). USDA is an equal opportunity provider and employer. NR 62 TC 0 Z9 0 U1 8 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD NOV PY 2016 VL 106 IS 11 BP 1426 EP 1437 DI 10.1094/PHYTO-03-16-0143-R PG 12 WC Plant Sciences SC Plant Sciences GA DZ5KI UT WOS:000385900600022 PM 27392175 ER PT J AU Diaz-Lara, A Martin, RR AF Diaz-Lara, Alfredo Martin, Robert R. TI Blueberry fruit drop-associated virus: A New Member of the Family Caulimoviridae Isolated From Blueberry Exhibiting Fruit-Drop Symptoms SO PLANT DISEASE LA English DT Article ID ROLLING-CIRCLE AMPLIFICATION; PLANT; SEQUENCE; PROTEIN; PARARETROVIRUS; PROMOTER; DATABASE; SEARCH; GENOME AB This study describes the nucleotide sequence and genome organization of a new DNA virus isolated from 'Bluecrop' blueberry plants exhibiting fruit-drop symptoms and named Blueberry fruit drop-associated virus (BFDaV). Blueberry fruit drop disease was first detected in blueberry plants in British Columbia, Canada in the late 1990s, and in a single field in northern Washington state in the United States in 2012. Infected bushes abort nearly 100% of their fruit about three weeks prior to harvest, when the berries are about 3 to 5 mm in diameter. At harvest, the affected plants appear taller than healthy ones as there is no fruit weighing down the branches. The virus was amplified from diseased material using rolling circle amplification, followed by enzyme digestion, cloning, and sequencing. The full genome of BFDaV is 9,850 bp in length and contains a single open reading frame, encoding for a polyprotein, and a large non coding region. Based on the genome size and organization and phylogenetics, BFDaV is proposed as a new and the largest member of family Caulimoviridae. Finally, in mapping part of a field with fruit-drop symptoms, there was a nearly perfect correlation between the presence of the virus and fruit-drop symptoms. C1 [Diaz-Lara, Alfredo] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Martin, Robert R.] USDA ARS, Hort Crops Res Unit, Corvallis, OR 97330 USA. RP Diaz-Lara, A (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM diazlara@oregonstate.edu FU United States Department of Agriculture Agricultural Research Service; Oregon Blueberry Commission; Washington Blueberry Commission; BC Blueberry Industry Development Council; National Clean Plant Network; APHIS Plant Pest and Disease Management and Disaster Prevention Program FX This work was funded by the United States Department of Agriculture Agricultural Research Service, Oregon Blueberry Commission, Washington Blueberry Commission, BC Blueberry Industry Development Council, National Clean Plant Network, and APHIS Plant Pest and Disease Management and Disaster Prevention Program. We thank D. F. Quito-Avila from the Centro de Investigaciones Biotecnologicas del Ecuador-ESPOL (Guayaquil, Ecuador) for helpful discussions. NR 24 TC 0 Z9 0 U1 4 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD NOV PY 2016 VL 100 IS 11 BP 2211 EP 2214 DI 10.1094/PDIS-06-16-0792-RE PG 4 WC Plant Sciences SC Plant Sciences GA DZ1OX UT WOS:000385608800004 ER PT J AU Yang, D Zhang, J Wu, MD Chen, WD Li, GQ Yang, L AF Yang, Dan Zhang, Jing Wu, Mingde Chen, Weidong Li, Guoqing Yang, Long TI Characterization of the Mycelial Compatibility Groups and Mating Type Alleles in Populations of Sclerotinia minor in Central China SO PLANT DISEASE LA English DT Article ID FUNGAL PLANT PATHOGEN; GENETIC DIVERSITY; FIELD POPULATIONS; LETTUCE DROP; 1ST REPORT; MARKERS; CANOLA; HOSTS; DIFFERENTIATION; HYPOVIRULENCE AB Ninety-five single-sclerotium isolates were obtained from lettuce and weeds in three counties in central China. They were identified belonging to Sclerotinia minor based on colony morphology and the S. minor-specific DNA marker. Mycelial compatibility groups (MCGs) and the mating type (MAT) alleles in these isolates were determined using the methods of paired cultures and specific PCR, respectively, and the MCG data were used to calculate Shannon's H index (H) and Simpson index (S), thereby evaluating diversity of S. minor. Eight MCGs (MCG1 to MCG8) and two MAT alleles (Inv+, Inv-) were identified in these isolates. Low diversity was detected for the total 95 isolates (H = 1.748, S = 0.786). Isolates of different MCGs or with different MAT alleles did not significantly differ (P > 0.05) in mycelial growth rate on potato dextrose agar (PDA, 20 degrees C) or lesion diameter on lettuce leaves (20 degrees C), but slightly differed in the number of sclerotia produced on PDA (20 degrees C). Furthermore, this study reported five new host plants of S. minor in China, including Capsella bursapastoris, Oenanthe javanica, Fragaria gracilis, Ranunculus ternatus, and Salvia plebeia, and identified three hypovirulent isolates. These results broaden our understanding about the population biology of S. minor. C1 [Yang, Dan; Zhang, Jing; Wu, Mingde; Li, Guoqing; Yang, Long] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China. [Yang, Dan; Zhang, Jing; Wu, Mingde; Li, Guoqing; Yang, Long] Huazhong Agr Univ, Hubei Key Lab Plant Pathol, Wuhan 430070, Peoples R China. [Chen, Weidong] Washington State Univ, USDA, ARS, Pullman, WA 99164 USA. RP Yang, L (reprint author), Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China.; Yang, L (reprint author), Huazhong Agr Univ, Hubei Key Lab Plant Pathol, Wuhan 430070, Peoples R China. EM yanglong@mail.hzau.edu.cn FU Fundamental Research Funds for the Central Universities [2662015PY040]; National Natural Science Foundation of China [31272084, 31471813]; China Agriculture Research System [CARS-13] FX This study was financially supported by the Fundamental Research Funds for the Central Universities (Grant No. 2662015PY040), the National Natural Science Foundation of China (Grant Nos. 31272084, 31471813), and the earmarked fund for China Agriculture Research System (Grant No. CARS-13). Kind appreciations are due to Dr. Barbara Shew of North Carolina State University for providing S. minor isolates. NR 44 TC 0 Z9 0 U1 7 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD NOV PY 2016 VL 100 IS 11 BP 2313 EP 2318 DI 10.1094/PDIS-12-15-1453-RE PG 6 WC Plant Sciences SC Plant Sciences GA DZ1OX UT WOS:000385608800018 ER PT J AU Fraedrich, SW Harrington, TC McDaniel, BA Best, GS AF Fraedrich, S. W. Harrington, T. C. McDaniel, B. A. Best, G. S. TI First Report of Laurel Wilt, Caused by Raffaelea lauricola, on Spicebush (Lindera benzoin) in South Carolina. SO PLANT DISEASE LA English DT News Item ID REDBAY AMBROSIA BEETLE C1 [Fraedrich, S. W.; Best, G. S.] US Forest Serv, Forestry Sci Lab, Athens, GA 30602 USA. [Harrington, T. C.] Iowa State Univ, Dept Plant Pathol & Microbiol, Ames, IA 50011 USA. [McDaniel, B. A.] Recreat & Pk Dept, Sumter, SC USA. RP Fraedrich, SW (reprint author), US Forest Serv, Forestry Sci Lab, Athens, GA 30602 USA. NR 5 TC 0 Z9 0 U1 4 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD NOV PY 2016 VL 100 IS 11 BP 2330 EP 2331 DI 10.1094/PDIS-05-16-0674-PDN PG 2 WC Plant Sciences SC Plant Sciences GA DZ1OX UT WOS:000385608800050 ER PT J AU Malaker, PK Banna, NCD Tiwari, TP Collis, WJ Duveiller, E Singh, PK Joshi, AK Singh, RP Braun, HJ Peterson, GL Pedley, KF Farman, ML Valent, B AF Malaker, P. K. Banna, N. C. D. Tiwari, T. P. Collis, W. J. Duveiller, E. Singh, P. K. Joshi, A. K. Singh, R. P. Braun, H. -J. Peterson, G. L. Pedley, K. F. Farman, M. L. Valent, B. TI First Report of Wheat Blast Caused by Magnaporthe otyzae Pathotype triticum in Bangladesh. SO PLANT DISEASE LA English DT News Item C1 [Malaker, P. K.; Banna, N. C. D.] Bangladesh Agr Res Inst, Wheat Res Ctr, Nashipur, Dinajpur, Bangladesh. [Tiwari, T. P.; Collis, W. J.] House 10B,Rd 53,Gulshan 2,POB 6057, Dhaka 1213, Gulshan, Bangladesh. [Duveiller, E.; Singh, P. K.; Joshi, A. K.; Singh, R. P.; Braun, H. -J.] Int Maize & Wheat Improvement Ctr CIMMYT, Mexico City 06600, DF, Mexico. [Peterson, G. L.; Pedley, K. F.] ARS, USDA, FDWSRU, Ft Detrick, MD 21702 USA. [Farman, M. L.] Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA. [Valent, B.] Kansas State Univ, Throckmorton Plant Sci Ctr, Dept Plant Pathol, Manhattan, KS 66506 USA. RP Malaker, PK (reprint author), Bangladesh Agr Res Inst, Wheat Res Ctr, Nashipur, Dinajpur, Bangladesh. NR 2 TC 2 Z9 2 U1 8 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD NOV PY 2016 VL 100 IS 11 BP 2330 EP 2330 DI 10.1094/PDIS-05-16-0666-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DZ1OX UT WOS:000385608800049 ER PT J AU Olatinwo, R Barton, C Fraedrich, SW Johnson, W Hwang, J AF Olatinwo, R. Barton, C. Fraedrich, S. W. Johnson, W. Hwang, J. TI First Report of Laurel Wilt, Caused by Raffaelea lauricola, on Sassafras (Sassafras albidum) in Arkansas. SO PLANT DISEASE LA English DT News Item ID SOUTHEASTERN UNITED-STATES; CURCULIONIDAE; SCOLYTINAE; COLEOPTERA; DISEASE; REDBAY C1 [Olatinwo, R.] US Forest Serv, USDA, Southern Res Stn, Pineville, LA 71360 USA. [Barton, C.] Arkansas Forestry Commiss, Little Rock, AR 72204 USA. [Fraedrich, S. W.] US Forest Serv, Forestry Sci Lab, Athens, GA 30602 USA. [Johnson, W.; Hwang, J.] US Forest Serv, USDA, Forest Hlth Protect, Pineville, LA 71360 USA. RP Olatinwo, R (reprint author), US Forest Serv, USDA, Southern Res Stn, Pineville, LA 71360 USA. NR 5 TC 1 Z9 1 U1 2 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD NOV PY 2016 VL 100 IS 11 BP 2331 EP 2332 DI 10.1094/PDIS-05-16-0697-PDN PG 2 WC Plant Sciences SC Plant Sciences GA DZ1OX UT WOS:000385608800052 ER PT J AU Fichtner, EJ Browne, GT Mortaz, M Ferguson, L Blomquist, CL AF Fichtner, E. J. Browne, G. T. Mortaz, M. Ferguson, L. Blomquist, C. L. TI First Report of Root Rot Caused by Phytopythium helicoides on Pistachio Rootstock in California. SO PLANT DISEASE LA English DT News Item C1 [Fichtner, E. J.] Univ Calif Cooperat Extens, Tulare Co, Tulare, CA 93274 USA. [Browne, G. T.] Univ Calif Davis, Dept Plant Pathol, USDA ARS, CPGRU, Davis, CA 95616 USA. [Mortaz, M.; Ferguson, L.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Blomquist, C. L.] CDFA, PPDC, Sacramento, CA 95832 USA. RP Fichtner, EJ (reprint author), Univ Calif Cooperat Extens, Tulare Co, Tulare, CA 93274 USA. NR 5 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD NOV PY 2016 VL 100 IS 11 BP 2337 EP 2337 DI 10.1094/PDIS-02-15-1424-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DZ1OX UT WOS:000385608800066 ER PT J AU Tan, SH Talibov, THO Krueger, RR Bodaghi, S Dang, T Chao, YY Greer, G Vidalakis, G AF Tan, S. H. Talibov, T. H. O. Krueger, R. R. Bodaghi, S. Dang, T. Chao, Y. Y. Greer, G. Vidalakis, G. TI First Report of Citrus Exocortis Viroid and Two Citrus Variants of the Hop Stunt Viroid on Lemon in Azerbaijan. SO PLANT DISEASE LA English DT News Item C1 [Tan, S. H.; Bodaghi, S.; Dang, T.; Chao, Y. Y.; Greer, G.; Vidalakis, G.] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. [Talibov, T. H. O.] Bioresources Inst, Nakhichivan Sect, Nakichivan, Azerbaijan. [Krueger, R. R.] ARS, USDA, Natl Clonal Germplasm Repository Citrus & Dates, Riverside, CA USA. RP Tan, SH (reprint author), Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. NR 5 TC 0 Z9 0 U1 2 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD NOV PY 2016 VL 100 IS 11 BP 2341 EP 2341 DI 10.1094/PDIS-05-16-0761-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DZ1OX UT WOS:000385608800076 ER PT J AU Heidari, M Wang, D Delekta, P Sun, SH AF Heidari, Mohammad Wang, Dan Delekta, Phillip Sun, Shuhong TI Marek's disease virus immunosuppression alters host cellular responses and immune gene expression in the skin of infected chickens SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article DE Marek's disease; Immunosuppression; Feather follicle epithelium; Adhesion molecules; CD8 glycoprotein; Cytokines ID IN-VIVO REPLICATION; RIBONUCLEOTIDE REDUCTASE; VACCINATED CHICKENS; VIRULENT MDV; T-CELLS; HERPESVIRUS; IDENTIFICATION; FEATHERS; PCR; TRANSFORMATION AB Marek's disease virus (MDV), a highly cell-associated lymphotropic alpha-herpesvirus, is the causative agent of Marek's disease (MD) in domestic chickens. MDV replicates in chicken lymphocytes and establishes a latent infection within CD4(+) T cells. The latently infected CD4(+) T cells carry the virus to visceral organs, peripheral nerves, and feather follicle epithelium (FFE). FFE is the only anatomical site where infectious enveloped cell-free virus particles are produced and disseminated into the environment. This study investigated the immunological responses and mechanism of viral-induced immunosuppression and immune evasion in the FFE. Strong viral replication and lack of a significant number of cytotoxic T lymphocytes (CTL) in the infected tissues was prominent. Although the overall gene expression pattern was suggestive of a Th1 type immune response, the expression levels of several key immune genes were down regulated in the infected tissues. The mechanism of MDV-induced immunosuppression appears to be through inhibition of CTL function due to down regulation of CD8 glycoprotein and/or blocking of CTL migration due to decrease expression of cell adhesion molecules. Published by Elsevier B.V. C1 [Heidari, Mohammad; Delekta, Phillip] ARS, USDA, Avian Dis & Oncol Lab, 4279 East Mt Hope Rd, E Lansing, MI 48823 USA. [Wang, Dan; Sun, Shuhong] Shandong Agr Univ, Coll Anim Sci & Vet Med, Tai An, Shandong, Peoples R China. RP Heidari, M (reprint author), ARS, USDA, Avian Dis & Oncol Lab, 4279 East Mt Hope Rd, E Lansing, MI 48823 USA. EM mohammad.heidari@ars.usda.gov NR 41 TC 0 Z9 0 U1 3 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 EI 1873-2534 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD NOV 1 PY 2016 VL 180 BP 21 EP 28 DI 10.1016/j.vetimm.2016.08.013 PG 8 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA DY7SA UT WOS:000385328400004 PM 27692091 ER PT J AU Palmer, MV Thacker, TC Waters, WR AF Palmer, Mitchell V. Thacker, Tyler C. Waters, W. Ray TI Multinucleated giant cell cytokine expression in pulmonary granulomas of cattle experimentally infected with Mycobacterium bovis SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article DE Cytokine; Giant cell; Granuloma; Mycobacterium bovis; RNAScope (R) ID IN-SITU HYBRIDIZATION; GENE-EXPRESSION; TUBERCULOSIS; PROGRESSION; APOPTOSIS; DISEASE; TISSUES; LESIONS; MICE; RNA AB Regardless of host, pathogenic mycobacteria of the Mycobacterium tuberculosis complex such as Mycobacterium bovis, induce a characteristic lesion known as a granuloma, tubercle or tuberculoid granuloma. Granulomas represent a distinct host response to chronic antigenic stimuli, such as foreign bodies, certain bacterial components, or persistent pathogens such as M. bovis. Granulomas are composed of specific cell types including epithelioid macrophages, lymphocytes and a morphologically distinctive cell type, the multinucleated giant cell. Multinucleated giant cells are formed by the fusion of multiple macrophages; however, their function remains unclear. In humans, giant cells in tuberculous granulomas have been shown to express various cytokines, chemokines and enzymes important to the formation and maintenance of the granuloma. The objective of this study was to quantitatively assess multinucleated giant cell cytokine expression in bovine tuberculoid granulomas; focusing on cytokines of suspected relevance to bovine tuberculosis. Using calves experimentally infected with M. bovis, in situ cytokine expression was quantitatively assessed using RNAScope (R) for the following cytokines TNF-alpha, IFN-gamma, TGF-beta, IL-17A and IL-10. Multinucleated giant cells in bovine tuberculoid granulomas expressed all examined cytokines to varying degrees, with differential expression of TGF-beta, IL-17A and IL-10 in giant cells from early versus late stage granulomas. There was a modest, positive correlation between the level of cytokine expression and cell size or number of nuclei. These results suggest that multinucleated giant cells are active participants within bovine tuberculoid granulomas, contributing to the cytokine milieu necessary to form and maintain granulomas. Published by Elsevier B.V. C1 [Palmer, Mitchell V.; Thacker, Tyler C.; Waters, W. Ray] ARS, Infect Bacterial Dis Livestock Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. RP Palmer, MV (reprint author), Natl Anim Dis Ctr, 1920 Dayton Ave, Ames, IA 50010 USA. EM mitchell.palmer@ars.usda.gov FU US Department of Agriculture, Agricultural Research Service Project (CRIS) [3625-32000-104] FX The authors thank A. Lasley, S. Zimmerman, H. Trenkamp and the animal care staff at NADC for excellent technical support and animal care. USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this manuscript is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. Research funds were provided by the US Department of Agriculture, Agricultural Research Service Project (CRIS# 3625-32000-104). NR 38 TC 0 Z9 0 U1 7 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 EI 1873-2534 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD NOV 1 PY 2016 VL 180 BP 34 EP 39 DI 10.1016/j.vetimm.2016.08.015 PG 6 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA DY7SA UT WOS:000385328400006 PM 27692093 ER PT J AU Moore, SJ Smith, JD Greenlee, MHW Nicholson, EM Richt, JA Greenlee, JJ AF Moore, S. J. Smith, J. D. Greenlee, M. H. West Nicholson, E. M. Richt, J. A. Greenlee, J. J. TI Comparison of Two US Sheep Scrapie Isolates Supports Identification as Separate Strains SO VETERINARY PATHOLOGY LA English DT Article DE intranasal administration; prion diseases; prion protein; PRNP; PrPSc proteins; scrapie; sheep; transmissible spongiform encephalopathies ID ABNORMAL PRION PROTEIN; GENETICALLY SUSCEPTIBLE SHEEP; CHRONIC WASTING DISEASE; IN-VITRO CONVERSION; NATURAL SCRAPIE; INCUBATION-TIME; SUFFOLK SHEEP; EXPERIMENTAL TRANSMISSION; PRP ACCUMULATION; INFECTED SHEEP AB Scrapie is a naturally occurring transmissible spongiform encephalopathy of sheep and goats. There are different strains of sheep scrapie that are associated with unique molecular, transmission, and phenotype characteristics. However, in the United States, very little is known about the potential presence of scrapie strains. Scrapie strain and PRNP genotype could both affect susceptibility, potential for transmission, incubation period (IP), and control measures required for eliminating scrapie from a flock. The investigators evaluated 2 US scrapie isolates, No. 13-7 and x124, after intranasal inoculation to compare clinical signs, IPs, spongiform lesions, and patterns of PrPSc deposition in sheep with scrapie-susceptible PRNP genotypes (QQ171). After inoculation with x124, susceptibility and IP were associated with valine at codon 136 (V136) of the prion protein: VV136 sheep had short IPs (6.9 months), those in AV136 sheep were 11.9 months, and AA136 sheep did not develop scrapie. All No. 13-7 inoculated sheep developed scrapie, with IPs of 20.1 months for AA136 sheep, 22.8 months for AV136 sheep, and 26.7 months for VV136 sheep. Patterns of immunoreactivity in the brain were influenced by inoculum isolate and host genotype. Differences in PrPSc profiles versus isolate were most striking when examining brains from sheep with the VV136 genotype. Inoculation into C57BL/6 mice resulted in markedly different attack rates (90.5% for x124 and 5.9% for No. 13-7). Taken together, these data demonstrate that No. 13-7 and x124 represent 2 distinct strains of scrapie with different IPs, genotype susceptibilities, and PrPSc deposition profiles. C1 [Moore, S. J.; Smith, J. D.; Nicholson, E. M.; Richt, J. A.; Greenlee, J. J.] ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. [Greenlee, M. H. West] Iowa State Univ, Dept Biomed Sci, Coll Vet Med, Ames, IA USA. [Smith, J. D.] Iowa State Univ, Dept Vet Pathol, Coll Vet Med, Ames, IA USA. [Richt, J. A.] Kansas State Univ, Coll Vet Med, Manhattan, KS 66506 USA. RP Greenlee, JJ (reprint author), ARS, Natl Anim Dis Ctr, USDA, 1920 Dayton Ave,POB 70, Ames, IA 50010 USA. EM justin.greenlee@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service FX This research was funded in its entirety by congressionally appropriated funds to the United States Department of Agriculture, Agricultural Research Service. The funders of the work did not influence study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 43 TC 0 Z9 0 U1 2 U2 2 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0300-9858 EI 1544-2217 J9 VET PATHOL JI Vet. Pathol. PD NOV PY 2016 VL 53 IS 6 BP 1187 EP 1196 DI 10.1177/0300985816629712 PG 10 WC Pathology; Veterinary Sciences SC Pathology; Veterinary Sciences GA DZ4HQ UT WOS:000385819700011 PM 26936223 ER PT J AU Sun, Y Bai, GY Wang, YX Zhang, YY Pan, J Cheng, WM Feng, XL Li, H Ma, CC Ruan, WB Shapiro-Ilan, DI AF Sun, Yang Bai, Guang-ying Wang, Ying-Xiao Zhang, Yuan-yuan Pan, Jie Cheng, Wei-min Feng, Xiao-ling Li, Hui Ma, Cheng-cang Ruan, Wei-bin Shapiro-Ilan, David I. TI The impact of Cu, Zn and Cr salts on the relationship between insect and plant parasitic nematodes: A reduction in biocontrol efficacy SO APPLIED SOIL ECOLOGY LA English DT Article DE Biocontrol; Meloidogyne incognita; Mortality; Soil health; Beneficial nematode ID HEAVY-METAL CONTAMINATION; ROOT-KNOT NEMATODES; SOIL FOOD-WEB; ENTOMOPATHOGENIC NEMATODES; MELOIDOGYNE-INCOGNITA; HETERORHABDITIS-BACTERIOPHORA; STEINERNEMA-CARPOCAPSAE; LABORATORY CONDITIONS; BIOLOGICAL-CONTROL; CADMIUM ACCUMULATION AB Accumulation and potential health risks of toxic elements are associated with excessive fertilizer and manure applications in vegetable production systems. The goal of the present study was to explore the effects of potential toxic elements on the function of a cohort of beneficial organisms, specifically, the insect parasitic nematodes known as entomopathogenic nematodes. We assessed the impact of heavy metal accumulation on entomopathogenic nematodes (Steinernema carpocapsae and Heterorhabditis bacteriophora) and plant parasitic nematodes (Meloidogyne incognita). Genearlly, pretreatment with 1.5 mg Cu/L, 50 mg Zn/L or 7.5 mg Cr/L solution for 24 h caused direct mortality of infective juvenile S. carpocapsae and H. bacteriophora nematodes, and lowered virulence of H. bacteriophora against Galleria mellonella. Interestingly, we found that 24 h exposure to 4.5 mg Cu/L, 50 mg Zn/L or 7.5 mg Cr/L solution already caused higher mortality in H. bacteriophora than M. incognita in a laboratory assay. Moreover, in a potted plant experiment, pretreatment of H. bacteriophora with Cu, Zn or Cr salts resulted in increased penetration of M. incognita into cucumber roots compared with H. bacteriophora that were not treated (thus reducing biocontrol efficacy). Pretreatment of S. carpocapsae with Cu also caused increased penetration of M. incognita into cucumber roots; however, none of the Zn and Cr pretreatment concentrations exposed to S. carpocapsae resulted in increased M. incognita root penetration, and lower concentrations of these elements caused decreased penetration (enhancing biocontrol efficacy). Our results indicate that heavy metals can have a direct negative effect on entomopathogenic nematodes (via direct mortality) and also an indirect effect by disrupting biocontrol efficacy against plant parasitic nematodes, but in some cases low concentration may stimulate biocontrol activity. High levels of heavy metal accumulation in greenhouse vegetable production systems could substantially influence nematode community structure and impact other soil biota as well. (C) 2016 Elsevier B.V. All rights reserved. C1 [Sun, Yang; Bai, Guang-ying; Wang, Ying-Xiao; Zhang, Yuan-yuan; Feng, Xiao-ling; Ruan, Wei-bin] Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China. [Pan, Jie] Tianjin Inst Agr Resources & Environm Sci, Tianjin 300192, Peoples R China. [Cheng, Wei-min] Minist Agr, Agroenvironm Protect Inst, Tianjin 300191, Peoples R China. [Li, Hui] Inst Agr Qual Stand & Testing Technol, Tianjin 300381, Peoples R China. [Ma, Cheng-cang] Tianjin Normal Univ, Coll Life Sci, Tianjin 300387, Peoples R China. [Shapiro-Ilan, David I.] USDA ARS, Southeastern Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. RP Ruan, WB (reprint author), Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China. EM ruanweibin2004@hotmail.com FU Special Fund for Agroscientific Research in the Public Interest [201103018]; 111 project [B08011]; Natural Science Foundation of China [31170412, 31470495]; Fundamental Research Funds for the Central Universities FX This work was jointly supported by the Special Fund for Agroscientific Research in the Public Interest (201103018) and 111 project (B08011), Natural Science Foundation of China (31170412 and 31470495) and Fundamental Research Funds for the Central Universities. We gratefully acknowledge the anonymous reviewers for their valuable comments on the manuscript. NR 66 TC 0 Z9 0 U1 26 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD NOV PY 2016 VL 107 BP 108 EP 115 DI 10.1016/j.apsoi1.2016.05.015 PG 8 WC Soil Science SC Agriculture GA DY1MZ UT WOS:000384860400011 ER PT J AU Zhu, SS Vivanco, JM Manter, DK AF Zhu, Shusheng Vivanco, Jorge M. Manter, Daniel K. TI Nitrogen fertilizer rate affects root exudation, the rhizosphere microbiome and nitrogen-use-efficiency of maize SO APPLIED SOIL ECOLOGY LA English DT Article DE Nitrogen-use-efficiency; Maize; Microbial ecology; Fertilizer; N immobilization ID BACTERIAL COMMUNITY STRUCTURE; 16S RIBOSOMAL-RNA; DIAZOTROPHIC BACTERIA; NUTRITIONAL-STATUS; SOIL MICROBIOME; PLANT; DENITRIFICATION; SUGARCANE; SEQUENCES; FIXATION AB The composition and function of microbial communities in the rhizosphere of crops have been linked to edaphic factors and root exudate composition. We examined the effect of N fertilizer (urea) rate on maize root exudation, the associated rhizosphere microbial community, and nitrogen-use-efficiency. Increasing N rate had a significant effect on root exudate quantity and composition. Specifically, the total abundance of sugars, sugar alcohols, and phenolics was positively correlated with N rate (p < 0.005). Similarly, the abundance of rhizosphere bacteria (16 S rRNA copies g(-1) soil FW) was enhanced with increasing N rate. Using PICRUSt, we also explored the metagenomic contribution of bacterial OTUs to the abundance of N cycle-related genes in-the maize rhizosphere. On a relative abundance basis, the nitrifying-(pmoA-amoA) and denitrifying-genes (nirK and nosZ) were significantly influenced by N rate (p <0.05); whereas, the nitrogen fixing (nifD and nifH) arid urease (ureC) genes were not influenced by N rate (p > 0.05). However, on a total abundance basis (gene copies g(-1) soil FW) all N-cycle genes increased significantly with increasing N rate (p < 0.05). Percent N recovery from both soil and fertilizer sources showed a curvilinear response that was highest at intermediate N rates; whereas, fertilizer N lost from the system increased significantly at the two highest N rates (p < 0.05). In summary, our results show high N rates increase both root exudation and the abundance of soil bacteria, which may help explain the decline in fertilizer-use efficiency and loss of N from the system at higher N rates. Published by Elsevier B.V. C1 [Zhu, Shusheng] Yunnan Agr Univ, Key Lab Agrobiodivers & Pest Management, Educ Minist China, Kunming 650201, Yunnan, Peoples R China. [Vivanco, Jorge M.] Colorado State Univ, Deptarment Hort & Landscape Architecture, Ft Collins, CO 80525 USA. [Manter, Daniel K.] ARS, Soil Plant Nutrient Res, USDA, Ft Collins, CO 80526 USA. RP Manter, DK (reprint author), ARS, Soil Plant Nutrient Res, USDA, Ft Collins, CO 80526 USA. EM daniel.manter@ars.usda.gov FU National Science Foundation of China [31260447]; "973" program from the Ministry of Science and Technology of China [2011CB100400] FX This study was partially supported by the National Science Foundation of China (31260447) and the "973" program from the Ministry of Science and Technology of China (2011CB100400). NR 69 TC 1 Z9 1 U1 83 U2 83 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD NOV PY 2016 VL 107 BP 324 EP 333 DI 10.1016/j.apsoi1.2016.07.009 PG 10 WC Soil Science SC Agriculture GA DY1MZ UT WOS:000384860400035 ER PT J AU Bera, T Collins, HP Alva, AK Purakayastha, TJ Patra, AK AF Bera, T. Collins, H. P. Alva, A. K. Purakayastha, T. J. Patra, A. K. TI Biochar and manure effluent effects on soil biochemical properties under corn production SO APPLIED SOIL ECOLOGY LA English DT Article DE Biochar; Manure; Biochemical properties; Soil quality ID MICROBIAL BIOMASS; TEMPERATE SOILS; BLACK CARBON; LOAM SOIL; QUALITY; AMENDMENT; MANAGEMENT; PYROLYSIS; EMISSIONS; PEANUT AB Biochar (BC), an aromatic carbon (C) rich compound has been used to sequester C in terrestrial ecosystems. Biochar has also been shown to improve soil fertility and crop productivity when applied to soil. Biochemical properties of soil are sensitive parameters that indicate the change in soil processes that result from soil management practices. Major knowledge gaps exist on the long term effects of biochar addition in cropped field soils. Therefore, a field experiment (3 yrs) was conducted on a Warden silt loam soil to investigate the changes in soil biochemical properties following three years of corn (Zea mays L.) production with applications of biochar (BC), fertilizer (NPK) or dairy manure (DE). The NPK or DE treatments were applied annually, while BC was applied only the first year. Organic carbon (TOC), total organic nitrogen (TON), TOC to TON ratio (CNR), soil microbial biomass carbon (MBC), Metabolic quotient (MQ), potential mineralizable nitrogen (PMN), fluorescein diacetate hydrolysis activity (FDA), acid phophatase activity (ACP), alkaline phosphatase activity (ALP), beta-glucosidase activity, urease activity (URA), and soil pH were evaluated at 0-15 and 15-30 cm depths after the 3rd year of corn production. A soil quality index equation was also developed with linear scoring functions. NPK or DE with BC application increased soil pH, TOC, MBC, FDA, CN ratio, ALP and URA while decreased MQ and ACP activity. Biochar had little effect on N dynamics in the soil. Application of biochar to both NPK and DE treatments improved the soil quality index as compared to that without biochar. (C) 2016 Elsevier B.V. All rights reserved. C1 [Bera, T.; Collins, H. P.; Alva, A. K.] USDA ARS, Vegetable & Forage Crops Res Unit, 24106 N Bunn Rd, Prosser, WA 99350 USA. [Collins, H. P.] USDA ARS, Grassland Soil & Water Lab, Temple, TX 76502 USA. [Alva, A. K.] USDA ARS, Agr Syst Res Unit, Sidney, MT 59270 USA. [Bera, T.; Purakayastha, T. J.; Patra, A. K.] ICAR Indian Agr Res Inst, Div Soil Sci & Agr Chem, New Delhi 110012, India. [Patra, A. K.] ICAR Indian Inst Soil Sci, Bhopal 462038, MP, India. RP Bera, T (reprint author), USDA ARS, Vegetable & Forage Crops Res Unit, 24106 N Bunn Rd, Prosser, WA 99350 USA. EM tanumoy.soil@gmail.com FU Fulbright Commission FX This project was conducted as part of the Fulbright-Nehru Doctoral and Professional Research Fellowship to Bera. We gratefully acknowledge the financial support from Fulbright Commission. Bera is also thankful to ICAR and IARI for permitting him to peruse the work in USA during his Ph.D. Bill Boge and Rebeca Cochran are acknowledged for their help in laboratory with the analysis of samples. Finally, we thank the editors and anonymous reviewers of Applied Soil Ecology Journal for their apt comments to improve this publication. NR 63 TC 0 Z9 0 U1 35 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD NOV PY 2016 VL 107 BP 360 EP 367 DI 10.1016/j.apsoil.2016.07.011 PG 8 WC Soil Science SC Agriculture GA DY1MZ UT WOS:000384860400038 ER PT J AU Callaham, MA Snyder, BA James, SW Oberg, ET AF Callaham, Mac A., Jr. Snyder, Bruce A. James, Samuel W. Oberg, Erik T. TI Evidence for ongoing introduction of non-native earthworms in the Washington, DC metropolitan area SO BIOLOGICAL INVASIONS LA English DT Article DE Earthworm invasion; Soil macroinvertebrates; Soil disturbance; Native species conservation; Public land management ID NORTH-AMERICA; EXOTIC EARTHWORMS; BOREAL FORESTS; INVASION; LUMBRICIDAE; OLIGOCHAETA; ACANTHODRILIDAE; MEGASCOLECIDAE; COMPETITION; MOUNTAINS AB Earthworm introductions and invasions are ongoing, with significant consequences for ecological characteristics and function where populations of invasive species reach high densities. In North America the influx of people, goods and materials to coastal cities has long been recognized to be related to introduction and establishment of many different invasive organisms. We conducted surveys for soil invertebrates in the Washington, DC area along the Potomac River corridor to examine the influence of historic soil profile disrupting disturbances on the composition of soil invertebrate communities. Here we report three earthworm taxa that either (1) had never been previously reported in North America (Lumbricidae: Helodrilus oculatus), (2) had never been reported from "wild" caught samples in forested soils (Lumbricidae: Eisenia fetida), or (3) represented a notable range expansion for an introduced species (Lumbricidae: Murchieona muldali). All three species reported here have attributes that give reason for concern over their expansion into North American soils, not least of which is their potential for competitive interactions with the remaining native earthworm species. C1 [Callaham, Mac A., Jr.] US Forest Serv, Ctr Forest Disturbance Sci, USDA, Southern Res Stn, Athens, GA 30602 USA. [Snyder, Bruce A.] Kansas State Univ, Div Biol, Ackert Hall, Manhattan, KS 66506 USA. [James, Samuel W.] Univ Iowa, Dept Biol, Iowa City, IA 52242 USA. [Oberg, Erik T.] Natl Pk Serv, Yellowstone Natl Pk, WY USA. RP Callaham, MA (reprint author), US Forest Serv, Ctr Forest Disturbance Sci, USDA, Southern Res Stn, Athens, GA 30602 USA. EM mcallaham@fs.fed.us NR 24 TC 0 Z9 1 U1 13 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD NOV PY 2016 VL 18 IS 11 BP 3133 EP 3136 DI 10.1007/s10530-016-1230-2 PG 4 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DY5RN UT WOS:000385159900007 ER PT J AU Pinto, SM Ortega, YK AF Pinto, Sarah M. Ortega, Yvette K. TI Native species richness buffers invader impact in undisturbed but not disturbed grassland assemblages SO BIOLOGICAL INVASIONS LA English DT Article DE Diversity; Drought; Exotic plants; Fire; Invasion ID KNAPWEED CENTAUREA-MACULOSA; PLANT DIVERSITY; COMMUNITY INVASIBILITY; PRESCRIBED FIRE; INVASIVE PLANT; BIODIVERSITY; ECOSYSTEMS; PRODUCTIVITY; COMPETITION; MANAGEMENT AB Many systems are prone to both exotic plant invasion and frequent natural disturbances. Native species richness can buffer the effects of invasion or disturbance when imposed in isolation, but it is largely unknown whether richness provides substantial resistance against invader impact in the face of disturbance. We experimentally examined how disturbance (drought/burning) influenced the impact of three exotic invaders (Centaurea stoebe, Linaria dalmatica, or Potentilla recta) on native abundance across a gradient of species richness, using previously constructed grassland assemblages. We found that invaders had higher cover in experimentally disturbed plots than in undisturbed plots across all levels of native species richness. Although exotic species varied in cover, all three invaders had significant impacts on native cover in disturbed plots. Regardless of disturbance, however, invader cover diminished with increasing richness. Invader impacts on native cover also diminished at higher richness levels, but only in undisturbed plots. In disturbed plots, invaders strongly impacted native cover across all richness levels, as disturbance favoured invaders over native species. By examining these ecological processes concurrently, we found that disturbance exacerbated invader impacts on native abundance. Although diversity provided a buffering effect against invader impact without disturbance, the combination of invasion and disturbance markedly depressed native abundance, even in high richness assemblages. C1 [Pinto, Sarah M.] Univ Montana, Dept Organismal Biol & Ecol, Missoula, MT 59812 USA. [Ortega, Yvette K.] US Forest Serv, USDA, Rocky Mt Res Stn, 800 E Beckwith Ave, Missoula, MT 59801 USA. RP Pinto, SM (reprint author), Univ Montana, Dept Organismal Biol & Ecol, Missoula, MT 59812 USA. EM sarahmariannepinto@gmail.com FU National Sciences and Engineering Research Council of Canada (NSERC PGS-D) FX We thank John Maron, Marilyn Marler, Ray Callaway, Dean Pearson, Winsor Lowe, Lisa Eby, and Dan Atwater for their extensive guidance and feedback. We thank many field assistants for their work on this project especially Jeremy Amberson, Katie Holder, Natalie Shapiro, and Crista Clark. We gratefully acknowledge the support of the National Sciences and Engineering Research Council of Canada (NSERC PGS-D). NR 61 TC 0 Z9 0 U1 44 U2 44 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD NOV PY 2016 VL 18 IS 11 BP 3193 EP 3204 DI 10.1007/s10530-016-1208-0 PG 12 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DY5RN UT WOS:000385159900012 ER PT J AU Porensky, LM Blumenthal, DM AF Porensky, Lauren M. Blumenthal, Dana M. TI Historical wildfires do not promote cheatgrass invasion in a western Great Plains steppe SO BIOLOGICAL INVASIONS LA English DT Article DE Bromus arvensis; Fire regime; Great Basin; Shrub-grass interactions; Thunder Basin National Grassland; Winter precipitation ID BROMUS-TECTORUM INVASION; MIXED-GRASS PRAIRIE; BURNING JAPANESE BROME; CLIMATE-CHANGE; BIOLOGICAL INVASIONS; NORTH-AMERICA; POPULATION-DYNAMICS; TALLGRASS PRAIRIE; PLANT COMMUNITY; SUMMER FIRE AB Plant invasion and wildfire are often tightly linked. Invasive grasses, in particular, can severely alter ecosystems by increasing fire frequency and intensity. In western North America, positive feedbacks between wildfire and Bromus tectorum (cheatgrass) invasion have contributed to widespread plant community conversion. Impacts of conversion include reduced biodiversity, wildlife habitat, and livestock weight gains, as well as increased costs associated with fire-fighting and ecosystem restoration. While B. tectorum has been studied intensively in the Intermountain West, it is unclear whether fire-invasion feedback cycles observed in the Great Basin operate similarly in the western Great Plains, where annual bromes coexist with fire-adapted native species. In a shrub-grass ecotone in northeastern Wyoming, we asked how wildfires have influenced B. tectorum and its congener, B. arvensis, and whether the effects of wildfire on annual bromes varied based on landscape context. We sampled annual bromes along 142 transects associated with 28 historical wildfires (2-26 years since fire). Both brome species were equally likely to occur in burned and unburned sites. Cover of B. tectorum was lower in burned sites. Soil texture, cover of other plant species, slope, and aspect were strongly associated with annual brome abundance. In the western Great Plains, single wildfires do not appear to promote B. tectorum invasion. However, the effects of repeated fires on invasion in this system remain unclear. Our findings stress that relationships between fire and plant invasion are governed not by invader identity alone but by ecosystem-specific interactions among invaders, fire regimes, and resident species. C1 [Porensky, Lauren M.; Blumenthal, Dana M.] USDA ARS, Rangeland Resources Res Unit, 1701 Ctr Ave, Ft Collins, CO 80526 USA. RP Porensky, LM (reprint author), USDA ARS, Rangeland Resources Res Unit, 1701 Ctr Ave, Ft Collins, CO 80526 USA. EM lauren.porensky@ars.usda.gov FU USDA-ARS FX Many thanks to the Thunder Basin Grassland Prairie Ecosystem Association, the US Forest Service Douglas Ranger District, cooperating private land owners, and Justin Derner for facilitating this research. William Armstrong helped to implement the stratified random sampling design. Thanks to Skye Greenler, Megan Gordon, Catherine Estep, Nickolas Dufek and Kevin Mueller for collecting data. David Augustine, Brian Anacker, Elizabeth Leger, Julie Kray, Eugene Schupp, and three anonymous reviewers provided assistance with the manuscript. Funding is from USDA-ARS. NR 69 TC 0 Z9 0 U1 28 U2 28 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD NOV PY 2016 VL 18 IS 11 BP 3333 EP 3349 DI 10.1007/s10530-016-1225-z PG 17 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DY5RN UT WOS:000385159900022 ER PT J AU Renye, JA Somkuti, GA Garabal, JI Steinberg, DH AF Renye, J. A., Jr. Somkuti, G. A. Garabal, J. I. Steinberg, D. H. TI Bacteriocin production by Streptococcus thermophilus in complex growth media SO BIOTECHNOLOGY LETTERS LA English DT Article DE Antimicrobial activity; Bacteriocin; Growth medium; Quorum sensing; Streptococcus thermophilus ID INHIBITORY SPECTRUM; MEDIUM COMPONENTS; SBT1277; PEPTIDE; STRAIN; BOZA AB To test if the production of bacteriocins by Streptococcus thermophilus is influenced when grown in various complex media commonly used for the culturing of lactic acid bacteria. Forty-one strains of S. thermophilus were screened for the production of bacteriocins in tryptone/yeast extract/lactose (TYL), M17-lactose (M17L), M17-glucose (M17G) and MRS media. Two strains, ST144 and ST145, were identified as novel bacteriocin producers, with constitutive production observed only in M17G. Strains ST110, ST114 and ST134 constitutively produced bacteriocins in all growth media but ST114 required growth in MRS for its antimicrobial activity to persist in a 24 h culture. The addition of a synthetic quorum sensing peptide (BlpC) induced bacteriocin production by ST106 in all media tested; and by ST118 in TYL and M17L. Strain ST109, which constitutively produced a bacteriocin in TYL and M17 broths, required BlpC induction when grown in MRS. Real-time PCR analysis showed that the natural expression of blpC in ST109 was lower when grown in MRS, suggesting that something in medium interfered with the blp quorum sensing system. As the choice of growth medium influences both bacteriocin production and peptide stability, several types of production media should be tested when screening for novel bacteriocin-producing strains of S. thermophilus. C1 [Renye, J. A., Jr.; Somkuti, G. A.; Steinberg, D. H.] ARS, Dairy & Funct Food Res Unit, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Garabal, J. I.] Agr Res Ctr Mabegondo CIAM, Xunta De Galicia 15318, Abegondo, Spain. RP Renye, JA (reprint author), ARS, Dairy & Funct Food Res Unit, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM john.renye@ars.usda.gov NR 24 TC 0 Z9 0 U1 13 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0141-5492 EI 1573-6776 J9 BIOTECHNOL LETT JI Biotechnol. Lett. PD NOV PY 2016 VL 38 IS 11 BP 1947 EP 1954 DI 10.1007/s10529-016-2184-2 PG 8 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DY5ZN UT WOS:000385184600015 PM 27515777 ER PT J AU Hubka, V Novakova, A Peterson, SW Frisvad, JC Sklenar, F Matsuzawa, T Kubatova, A Kolarik, M AF Hubka, Vit Novakova, Alena Peterson, Stephen W. Frisvad, Jens C. Sklenar, Frantisek Matsuzawa, Tetsuhiro Kubatova, Alena Kolarik, Miroslav TI A reappraisal of Aspergillus section Nidulantes with descriptions of two new sterigmatocystin-producing species SO PLANT SYSTEMATICS AND EVOLUTION LA English DT Article DE Aspergillus nidulans; Codon usage; Emericella; Mycotoxins; Paralogous genes; Subgeneric classification of Aspergillus ID EGYPTIAN DESERT SOIL; INVASIVE ASPERGILLOSIS; EMERICELLA-QUADRILINEATA; TRANSPLANT RECIPIENTS; PHYLOGENETIC ANALYSIS; GENUS ASPERGILLUS; DNA-SEQUENCES; AFLATOXIN B-1; SYNOPTIC KEY; SP NOV. AB Aspergillus section Nidulantes is a speciose group of microscopic fungi whose species are important in indoor air quality, food spoilage, mycotoxin production and human pathogenicity. We assembled as many species from the section as possible with either type specimens or protologues for analysis. DNA sequences were obtained from up to four loci to conduct phylogenetic analysis of sect. Nidulantes and other species in subgenus Nidulantes. Comparisons of phenetic characters were made. Our goal was to recognize monophyletic taxa for section Nidulantes as well as the other taxa in the subgenus. Phylogenetic analysis indicated that sections Versicolores and Aenei formed subclades in the larger clade containing the type species of section Nidulantes. In order to maintain monophyly in section Nidulantes, we synonymized sects. Versicolores and Aenei with sect. Nidulantes. In place of sections, we have identified six subclades in sect. Nidulantes that also have shared phenetic character sets that allow for easier morphological identification of species. Earlier reports showed that standard beta-tubulin primers can occasionally amplify the tubC gene rather than the benA gene they were designed for. We show that codon usage analysis can easily distinguish between these paralogues, and we provide primer pair options to allow benA amplifications. Two new sect. Nidulantes species are described from European caves, A. croceus (Spain) and A. askiburgiensis (Czech Republic). The analysis of exometabolites showed that both species are able to produce the mycotoxin sterigmatocystin and some additional compounds. C1 [Hubka, Vit; Sklenar, Frantisek; Kubatova, Alena; Kolarik, Miroslav] Charles Univ Prague, Dept Bot, Fac Sci, Benatska 2, Prague 12801 2, Czech Republic. [Hubka, Vit; Novakova, Alena; Sklenar, Frantisek; Kolarik, Miroslav] Inst Microbiol AS CR, Vvi, Videnska 1083, Prague 14220 4, Czech Republic. [Peterson, Stephen W.] ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 North Univ St, Peoria, IL 61604 USA. [Frisvad, Jens C.] Tech Univ Denmark, DTU Syst Biol, Bldg 221, DK-2800 Lyngby, Denmark. [Matsuzawa, Tetsuhiro] Nagasaki Univ, 1-1-1 Manabino, Nagoya, Nagasaki 8512195, Japan. RP Hubka, V (reprint author), Charles Univ Prague, Dept Bot, Fac Sci, Benatska 2, Prague 12801 2, Czech Republic.; Hubka, V (reprint author), Inst Microbiol AS CR, Vvi, Videnska 1083, Prague 14220 4, Czech Republic. EM hubka@biomed.cas.cz RI Kubatova, Alena/D-7918-2016; Kolarik, Miroslav/A-1680-2008 OI Kubatova, Alena/0000-0002-2837-1087; Kolarik, Miroslav/0000-0003-4016-0335 FU Ministry of Education, Youth and Sports of the Czech Republic (SVV project); project "BIOCEV-Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University" from the European Regional Development Fund [CZ.1.05/1.1.00/02.0109]; Charles University Grant Agency [GAUK 1130214]; Czech Science Foundation [P506-12-1064] FX This research was supported by the Ministry of Education, Youth and Sports of the Czech Republic (SVV project) and by the project "BIOCEV-Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University" (CZ.1.05/1.1.00/02.0109) from the European Regional Development Fund. Molecular genetic analyses were supported by the projects of Charles University Grant Agency (GAUK 1130214). The survey in European caves was supported through a grant from the Czech Science Foundation (Grant No. P506-12-1064). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. Prof Karol Marhold's consultation on nomenclatural issues is gratefully acknowledged. We thank Dr M. Chudickova and A. Gabrielova, for their invaluable assistance in laboratory, and Dr Z. Jurjevic for valuable comments. NR 123 TC 2 Z9 2 U1 13 U2 13 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0378-2697 EI 1615-6110 J9 PLANT SYST EVOL JI Plant Syst. Evol. PD NOV PY 2016 VL 302 IS 9 BP 1267 EP 1299 DI 10.1007/s00606-016-1331-5 PG 33 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA DY6BX UT WOS:000385192100008 ER PT J AU Stenger, DC Krugner, R Nouri, S Ferriol, I Falk, BW Sisterson, MS AF Stenger, Drake C. Krugner, Rodrigo Nouri, Shahideh Ferriol, Inmaculada Falk, Bryce W. Sisterson, Mark S. TI Sequence polymorphism in an insect RNA virus field population: A snapshot from a single point in space and time reveals stochastic differences among and within individual hosts SO VIROLOGY LA English DT Article DE Virus microevolution; Mutant spectrum; Dicistrovirus ID GLASSY-WINGED SHARPSHOOTER; VESICULAR STOMATITIS-VIRUS; STREAK-MOSAIC-VIRUS; WEST-NILE-VIRUS; HOMALODISCA-VITRIPENNIS; GENETIC-VARIATION; EVOLUTIONARY GENETICS; TEMPORAL DYNAMICS; BLUETONGUE VIRUS; MUTATION AB Population structure of Homalodisca coagulata Virus-1 (HoCV-1) among and within field-collected insects sampled from a single point in space and time was examined. Polymorphism in complete consensus sequences among single-insect isolates was dominated by synonymous substitutions. The mutant spectrum of the C2 helicase region within each single-insect isolate was unique and dominated by nonsynonymous singletons. Bootstrapping was used to correct the within-isolate nonsynonymous:synonymous arithmetic ratio (N:S) for RT-PCR error, yielding an N:S value similar to one log-unit greater than that of consensus sequences. Probability of all possible single-base substitutions for the C2 region predicted N:S values within 95% confidence limits of the corrected within-isolate N:S when the only constraint imposed was viral polymerase error bias for transitions over transversions. These results indicate that bottlenecks coupled with strong negative/purifying selection drive consensus sequences toward neutral sequence space, and that most polymorphism within single-insect isolates is composed of newly-minted mutations sampled prior to selection. Published by Elsevier Inc. C1 [Stenger, Drake C.; Krugner, Rodrigo; Sisterson, Mark S.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. [Nouri, Shahideh; Ferriol, Inmaculada; Falk, Bryce W.] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. RP Stenger, DC (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM drake.stenger@ars.usda.gov FU USDA-ARS [2034-22000-010-00D] FX We thank Kunbo Zhang and Tera L. Pitman for technical support. D.C. Stenger expresses gratitude to Roy French for a decade of insightful discussions on virus evolution that greatly influenced experimental design and analysis of the data presented here. Funding for this research was from USDA-ARS appropriated research project 2034-22000-010-00D. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not constitute endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 44 TC 0 Z9 0 U1 2 U2 2 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD NOV PY 2016 VL 498 BP 209 EP 217 DI 10.1016/j.virol.2016.08.026 PG 9 WC Virology SC Virology GA DY1RV UT WOS:000384873000022 PM 27598532 ER PT J AU Mascarin, GM Jaronski, ST AF Mascarin, Gabriel Moura Jaronski, Stefan T. TI The production and uses of Beauveria bassiana as a microbial insecticide SO WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY LA English DT Review DE Mycoinsecticides; Fermentation; Pests; Formulation; Blastospores; Conidia; White muscardine; Biocontrol ID METARHIZIUM-FLAVOVIRIDE CONIDIA; WEEVIL COSMOPOLITES-SORDIDUS; LYGUS-LINEOLARIS HETEROPTERA; BIOLOGICAL-CONTROL AGENTS; ASIAN LONGHORNED BEETLE; OIL-BASED FORMULATION; ENTOMOPATHOGENIC FUNGI; BEMISIA-TABACI; ENDOPHYTIC COLONIZATION; LIQUID CULTURE AB Among invertebrate fungal pathogens, Beauveria bassiana has assumed a key role in management of numerous arthropod agricultural, veterinary and forestry pests. Beauveria is typically deployed in one or more inundative applications of large numbers of aerial conidia in dry or liquid formulations, in a chemical paradigm. Mass production is mainly practiced by solid-state fermentation to yield hydrophobic aerial conidia, which remain the principal active ingredient of mycoinsecticides. More robust and cost-effective fermentation and formulation downstream platforms are imperative for its overall commercialization by industry. Hence, where economics allow, submerged liquid fermentation provides alternative method to produce effective and stable propagules that can be easily formulated as dry stable preparations. Formulation also continues to be a bottleneck in the development of stable and effective commercial Beauveria-mycoinsecticides in many countries, although good commercial formulations do exist. Future research on improving fermentation and formulation technologies coupled with the selection of multi-stress tolerant and virulent strains is needed to catalyze the widespread acceptance and usefulness of this fungus as a cost-effective mycoinsecticide. The role of Beauveria as one tool among many in integrated pest management, rather than a stand-alone management approach, needs to be better developed across the range of crop systems. Here, we provide an overview of mass-production and formulation strategies, updated list of registered commercial products, major biocontrol programs and ecological aspects affecting the use of Beauveria as a mycoinsecticide. C1 [Mascarin, Gabriel Moura] EMBRAPA Rice & Beans, Rod GO-462,Km 12, BR-75375000 St Antonio De Goias, Go, Brazil. [Jaronski, Stefan T.] ARS, USDA, Pest Management Res Unit, Northern Plains Agr Res Lab, 1500 N Cent Ave, Sidney, MT 59270 USA. RP Mascarin, GM (reprint author), EMBRAPA Rice & Beans, Rod GO-462,Km 12, BR-75375000 St Antonio De Goias, Go, Brazil. EM gmmascar@gmail.com RI Mascarin, Gabriel/E-4646-2015 OI Mascarin, Gabriel/0000-0003-3517-6826 FU EMBRAPA [02.11.07.005.00.00, 02.13.12.003.00.00] FX We thank Mark A. Jackson (USDA, Peoria) and anonymous reviewers for their valuable comments and suggestions. This work was partly supported by EMBRAPA's research Grants No 02.11.07.005.00.00 and 02.13.12.003.00.00. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 158 TC 0 Z9 0 U1 48 U2 48 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0959-3993 EI 1573-0972 J9 WORLD J MICROB BIOT JI World J. Microbiol. Biotechnol. PD NOV PY 2016 VL 32 IS 11 AR 177 DI 10.1007/s11274-016-2131-3 PG 26 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DY5NB UT WOS:000385145400004 PM 27628337 ER PT J AU Mills, NJ Beers, EH Shearer, PW Unruh, TR Amarasekare, KG AF Mills, Nicholas J. Beers, Elizabeth H. Shearer, Peter W. Unruh, Thomas R. Amarasekare, Kaushalya G. TI Comparative analysis of pesticide effects on natural enemies in western orchards: A synthesis of laboratory bioassay data SO BIOLOGICAL CONTROL LA English DT Article DE Aphelinus mali; Chrysoperla carnea; Deraeocoris brevis; Galendromus occidentalis; Hippodamia convergens; Trioxys pallidus ID ECOLOGICAL RISK-ASSESSMENT; TABLE RESPONSE EXPERIMENTS; POPULATION-GROWTH RATE; PEST-MANAGEMENT; LADY BEETLE; LAMBDA-CYHALOTHRIN; BIOLOGICAL-CONTROL; BIOCONTROL AGENTS; INSECTICIDES; EXPOSURE AB Pesticides are commonly used for pest management in apple, pear and walnut orchards in the western U.S. and may disrupt biological control of secondary pests in these crops. A comparative analysis was made of results obtained from a series of laboratory bioassays of acute mortality and life table response experiments to estimate lethal and sublethal effects of eight pesticides on seven natural enemy species through use of stage-structured population models. Even though a number of the pesticides tested were reduced-risk products, all of them with the exception of copper plus mancozeb and chlorantraniliprole, caused more than 80% acute mortality of at least one life stage of at least one of the natural enemy species at a full field-rate concentration and could thus be considered moderately harmful according to the International Organization for Biological Control classification for laboratory bioassays. Important sublethal effects included reductions in daily fecundity and egg fertility. From integration of the lethal and sublethal effects in matrix models, the mean of the estimated intrinsic rates of increase for natural enemy species was negative for exposure to cyantraniliprole, lambda-cyhalothrin and spinetoram, but positive and not significantly different from the control for exposure to chlorantraniliprole, copper plus mancozeb, novaluron, and sulfur. For comparisons among pesticides, there appears to be considerable variation in response among natural enemy species that can only be represented effectively from a full life table response experiment and a population-level endpoint, whereas among natural enemy species, their population-level response to the range of pesticides tested could frequently be represented by acute adult mortality alone. (C) 2016 Elsevier Inc. All rights reserved. C1 [Mills, Nicholas J.] Univ Calif Berkeley, Dept Environm Sci & Policy Management, Mulford Hall, Berkeley, CA 94720 USA. [Beers, Elizabeth H.] Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. [Shearer, Peter W.; Amarasekare, Kaushalya G.] Oregon State Univ, Mid Columbia Agr Res & Extens Ctr, 3005 Expt Stn Dr, Hood River, OR 97031 USA. [Unruh, Thomas R.] USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. RP Mills, NJ (reprint author), Univ Calif Berkeley, Dept Environm Sci & Policy Management, Mulford Hall, Berkeley, CA 94720 USA. EM nmills@berkeley.edu FU USDA-SCRI grant [2008-04854]; Washington Tree Fruit Research Commission; California Walnut Board; pear growers in Hood River (Oregon) FX This research was supported by USDA-SCRI grant 2008-04854 and matching funds from the Washington Tree Fruit Research Commission, California Walnut Board and pear growers in Hood River (Oregon). We thank DuPont Crop Protection, Dow Agro Sciences LLC, Chemtura AgroSolutions, Syngenta LLC and Micro Flo Company LLC for providing the pesticides used for this study. The research personnel and technical support of the laboratories from the University of California at Berkeley, Oregon State University, Washington State University and USDA-ARS Yakima are gratefully acknowledged. NR 56 TC 11 Z9 11 U1 25 U2 25 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD NOV PY 2016 VL 102 BP 17 EP 25 DI 10.1016/j.biocontrol.2015.05.006 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DW8XG UT WOS:000383938200003 ER PT J AU Bigot-Clivot, A Ladeiro, MP Lepoutre, A Bastien, F Bonnard, I Dubey, JP Villena, I Aubert, D Geffard, O Francois, A Geffard, A AF Bigot-Clivot, Aurelie Ladeiro, Melissa Palos Lepoutre, Alexandra Bastien, Fanny Bonnard, Isabelle Dubey, Jitender P. Villena, Isabelle Aubert, Dominique Geffard, Olivier Francois, Adeline Geffard, Alain TI Bioaccumulation of Toxoplasma and Cryptosporidium by the freshwater crustacean Gammarus fossarum: Involvement in biomonitoring surveys and trophic transfer SO ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY LA English DT Article DE Protozoa; Oocysts; Gammarids; Molecular detection ID MUSSELS DREISSENA-POLYMORPHA; OYSTERS CRASSOSTREA-VIRGINICA; DIGESTIVE ENZYME-ACTIVITIES; POLYMERASE-CHAIN-REACTION; CLAMS CORBICULA-FLUMINEA; MARINE BIVALVE SHELLFISH; ENHYDRA-LUTRIS-NEREIS; LONG-TERM SURVIVAL; REAL-TIME PCR; GONDII OOCYSTS AB The protozoa Toxoplasma gondii and Cryptosporidium parvum are public health priorities because their oocysts can persist in recreational, surface, drinking, river, and sea water sources for a long time. To evaluate the capacity of the freshwater crustacean Gammarus fossarum to accumulate T. gondii and C. parvum oocysts, gammarids were exposed to 200, 2000 or 20,000 oocysts per gammarid and per day for 21 days followed by 5 days of depuration. C parvum DNA was detected by qPCR in G. fossarum in only one out of four pools for the highest concentration and after 14 days of exposure, and T. gondii DNA was detected after 7 days of exposure to the two highest concentrations. Our results document the capacity of G. fossarum to accumulate T gondii in its tissues proportionally to the ambient concentration; the maximum number of oocysts was detected in gammarid tissues after exposure to 20,000 oocysts per day. Mean values of 3.26 ( +/- 3), 21.71 ( +/- 15.18), and 17.41 ( +/- 10.89) oocysts were detected in gammarids after 7, 14, and 21 days, respectively, and after 5 days of depuration, I gondii oocysts were still present in gammarid tissues. These results show for the first time that a freshwater crustacean can bioaccumulate T. gondii oocysts, suggesting that G. fossarum is a potential effective bioindicator of protozoan contamination in biomonitoring studies. Moreover, due to its key position in freshwater food webs, G. fossarum could also play a role in the trophic transfer of protozoa. (C) 2016 Elsevier Inc. All rights reserved. C1 [Bigot-Clivot, Aurelie; Ladeiro, Melissa Palos; Lepoutre, Alexandra; Bastien, Fanny; Bonnard, Isabelle; Geffard, Alain] Univ Reims, Unite Stress Environm & BIOSurveillance Milieux A, UMR SEBIO I 02, Reims, France. [Villena, Isabelle; Aubert, Dominique] Hop Maison Blanche Reims, SFR CAP Sante FED 4231, EA 3800, Lab Parasitol Mycol, Reims, France. [Dubey, Jitender P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Geffard, Olivier; Francois, Adeline] Inst Natl Rech Sci & Technol Environm & Agr, Unite Rech Milieux Aquat Ecol & Pollut, Ecotoxicol, Villeurbanne, France. RP Bigot-Clivot, A (reprint author), Univ Reims, Unite Stress Environm & BIOSurveillance Milieux A, UMR SEBIO I 02, Reims, France. EM aurelie.bigot@univ-reims.fr FU Agence nationale de securite sanitaire de l'alimentation, de l'environnement et du travail [2014/1/009 INQUASAN] FX The study was supported by the "Agence nationale de securite sanitaire de l'alimentation, de l'environnement et du travail" (projet no 2014/1/009 INQUASAN). NR 50 TC 0 Z9 0 U1 12 U2 14 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0147-6513 EI 1090-2414 J9 ECOTOX ENVIRON SAFE JI Ecotox. Environ. Safe. PD NOV PY 2016 VL 133 BP 188 EP 194 DI 10.1016/j.ecoenv.2016.07.006 PG 7 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DX5DA UT WOS:000384398800021 PM 27454203 ER PT J AU Ponsankar, A Vasantha-Srinivasan, P Senthil-Nathan, S Thanigaivel, A Edwin, ES Selin-Rani, S Kalaivani, K Hunter, WB Alessandro, RT Abdel-Megeed, A Paik, CH Duraipandiyan, V Al-Dhabi, NA AF Ponsankar, Athirstam Vasantha-Srinivasan, Prabhakaran Senthil-Nathan, Sengottayan Thanigaivel, Annamalai Edwin, Edward-Sam Selin-Rani, Selvaraj Kalaivani, Kandaswamy Hunter, Wayne B. Alessandro, Rocco T. Abdel-Megeed, Ahmed Paik, Chae-Hoon Duraipandiyan, Veeramuthu Al-Dhabi, Naif Abdullah TI Target and non-target toxicity of botanical insecticide derived from Couroupita guianensis L. flower against generalist herbivore, Spodoptera litura Fab. and an earthworm, Eisenia foetida Savigny SO ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY LA English DT Article DE Cannonball flower; Tropical armyworm secondary metabolites; Octacosane larval mortality; Food consumption; Earthworm; Histology; Longevity ID ANOPHELES-STEPHENSI; LITTORALIS LEP.; ESSENTIAL OIL; NOCTUIDAE; ANTIFEEDANT; EXTRACTS; GROWTH; LARVAE; LEPIDOPTERA; HUBNER AB Botanical insecticides may provide alternatives to synthetic insecticides for controlling Spodoptera litura (F.) and they are target specific, biodegradable, and harmless to mammals. Eight natural chemical compounds with larvicidal activity were identified from fraction F-6 of C. guianensis flower extract Probit analysis of 95% confidence level exposed an LC50 of 223 ppm against S. litura third instar larvae. The growth and development of S. titura was affected in sub-lethal concentrations of fraction F-6 (50, 100, 150 and 200 ppm) compared to controls. Similarly nutritional indices values decreased significantly compared to controls. Fraction F-6 also damaged the gut epithelial layer and brush border membrane (BBM). This study also resolved the effects of toxicity to non-target earthworm treated with fraction F-6 and chemical pesticides (monotrophos and cypermethrin) and the results showed that fraction F-6 had no harmful effect on E. fetida. Further, fraction F-6 was eluted and sub fractions F-6c (50 ppm) showed high mortality against S. litura third instar larvae. Octacosane from fraction F-6c was established and confirmed using IR spectrum and HPLC. The time of retention of fraction F-6c was confirmed with the octacosane standard. Fraction F-6 of C guianensis extract caused dose-dependent mortality towards S. litura Octacosane in fraction F-6c was establish to be the prominent chemical compound associated with causing mortality but other compounds present in the fraction F-6 were shown to be associated with changes in development of S. litura at low dosages. S. litura at low dosage. Therefore, these findings suggest that octacosane may be one of the major insecticidal compounds affecting S. litura survival. (C) 2016 Elsevier Inc. All rights reserved. C1 [Ponsankar, Athirstam; Vasantha-Srinivasan, Prabhakaran; Senthil-Nathan, Sengottayan; Thanigaivel, Annamalai; Edwin, Edward-Sam; Selin-Rani, Selvaraj] Manonmaniam Sundaranar Univ, Sri Paramakalyani Ctr Excellence Environm Sci, Div Biopesticides & Environm Toxicol, Tirunelveli 627412, Tamil Nadu, India. [Kalaivani, Kandaswamy] Sri Parasakthi Coll Women, Post Grad & Res Dept Zool, Tirunelveli 627802, Tamil Nadu, India. [Hunter, Wayne B.] USDA, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. [Alessandro, Rocco T.] Treasure Coast Chem Consultants LLC, 107 Lakes End Dr,Apt B, Ft Pierce, FL 34982 USA. [Abdel-Megeed, Ahmed] Univ Alexandria, Fac Agr, Dept Plant Protect, POB 21531, Alexandria 21526, Egypt. [Paik, Chae-Hoon] Natl Inst Crop Sci, Planning & Coordinat Div, 181 Hyeoksin Ro, Wanju Gun 565851, Jeollabuk Do, South Korea. [Duraipandiyan, Veeramuthu; Al-Dhabi, Naif Abdullah] King Saud Univ, Coll Sci, Environm Studies, Dept Bot & Microbiol, POB 2455, Riyadh 11451, Saudi Arabia. RP Senthil-Nathan, S (reprint author), Manonmaniam Sundaranar Univ, Sri Paramakalyani Ctr Excellence Environm Sci, Div Biopesticides & Environm Toxicol, Tirunelveli 627412, Tamil Nadu, India. EM senthil@msuniv.ac.in RI Veeramuthu, Duraipandiyan/D-9924-2012; OI Veeramuthu, Duraipandiyan/0000-0002-2107-3503; Abdel-Megeed, Ahmed/0000-0002-9777-4808 FU King Saud University FX This research was full financially supported by the King Saud University, through Vice Deanship of Research Chairs. NR 54 TC 0 Z9 0 U1 10 U2 10 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0147-6513 EI 1090-2414 J9 ECOTOX ENVIRON SAFE JI Ecotox. Environ. Safe. PD NOV PY 2016 VL 133 BP 260 EP 270 DI 10.1016/j.ecoenv.2016.06.043 PG 11 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DX5DA UT WOS:000384398800030 PM 27476000 ER PT J AU Maynard, JJ Karl, JW Browning, DM AF Maynard, Jonathan J. Karl, Jason W. Browning, Dawn M. TI Effect of spatial image support in detecting long-term vegetation change from satellite time-series SO LANDSCAPE ECOLOGY LA English DT Article DE Time series; MODIS; Landsat; Image support; Arid ecosystems; Breaks for additive season and trend ID LANDSAT SURFACE REFLECTANCE; STRUCTURAL-CHANGE MODELS; FIRE RISK-ASSESSMENT; REMOTE-SENSING DATA; HERBACEOUS BIOMASS; NATIONAL-PARK; NOAA-AVHRR; ECOSYSTEM; NDVI; RANGELANDS AB Arid rangelands have been severely degraded over the past century. Multi-temporal remote sensing techniques are ideally suited to detect significant changes in ecosystem state; however, considerable uncertainty exists regarding the effects of changing image resolution on their ability to detect ecologically meaningful change from satellite time-series. (1) Assess the effects of image resolution in detecting landscape spatial heterogeneity. (2) Compare and evaluate the efficacy of coarse (MODIS) and moderate (Landsat) resolution satellite time-series for detecting ecosystem change. Using long-term (similar to 12 year) vegetation monitoring data from grassland and shrubland sites in southern New Mexico, USA, we evaluated the effects of changing image support using MODIS (250-m) and Landsat (30-m) time-series in modeling and detecting significant changes in vegetation using time-series decomposition techniques. Within our study ecosystem, landscape-scale (> 20-m) spatial heterogeneity was low, resulting in a similar ability to detect vegetation changes across both satellite sensors and levels of spatial image support. While both Landsat and MODIS imagery were effective in modeling temporal dynamics in vegetation structure and composition, MODIS was more strongly correlated to biomass due to its cleaner (i.e., fewer artifacts/data gaps) 16-day temporal signal. The optimization of spatial/temporal scale is critical in ensuring adequate detection of change. While the results presented in this study are likely specific to arid shrub-grassland ecosystems, the approach presented here is generally applicable. Future analysis is needed in other ecosystems to assess how scaling relationships will change under different vegetation communities that range in their degree of landscape heterogeneity. C1 [Maynard, Jonathan J.; Karl, Jason W.; Browning, Dawn M.] New Mexico State Univ, USDA ARS, Jornada Expt Range, MSC 3JER, POB 30003, Las Cruces, NM 88003 USA. RP Maynard, JJ (reprint author), New Mexico State Univ, USDA ARS, Jornada Expt Range, MSC 3JER, POB 30003, Las Cruces, NM 88003 USA. EM jmaynard@nmsu.edu NR 48 TC 0 Z9 0 U1 19 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD NOV PY 2016 VL 31 IS 9 BP 2045 EP 2062 DI 10.1007/s10980-016-0381-y PG 18 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DX5RB UT WOS:000384438000010 ER PT J AU Levi, MR Bestelmeyer, BT AF Levi, Matthew R. Bestelmeyer, Brandon T. TI Biophysical influences on the spatial distribution of fire in the desert grassland region of the southwestern USA SO LANDSCAPE ECOLOGY LA English DT Article DE Fire probability; Ecological sites; Soil properties; Desert grasslands; Chihuahuan Desert; Madrean Archipelago; Multi-model inference ID WESTERN UNITED-STATES; NORTHERN CHIHUAHUAN DESERT; VEGETATION DYNAMICS; WILDFIRE IGNITIONS; PRESCRIBED FIRE; SONORAN DESERT; MOJAVE DESERT; CLIMATE; RESTORATION; MODELS AB Fire is an important driver of ecological processes in semiarid systems and serves a vital role in shrub-grass interactions. In desert grasslands of the southwestern US, the loss of fire has been implicated as a primary cause of shrub encroachment. Where fires can currently be re-introduced given past state changes and recent restoration actions, however, is unknown and controversial. Our objective was to evaluate the interactive effects of climate, urban development, and topo-edaphic properties on fire distribution in the desert grassland region of the southwestern United States. We characterized the spatial distribution of fire in the Chihuahuan Desert and Madrean Archipelago ecoregions and investigated the influence of soil properties and ecological site groups compared to other commonly used biophysical variables using multi-model inference. Soil-landscape properties significantly influenced the spatial distribution of fire ignitions. Fine-textured bottomland ecological site classes experienced more fires than expected in contrast to upland sites with coarse soil textures and high fragment content that experienced fewer fire ignitions than expected. Influences of mean annual precipitation, distance to road/rail, soil available water holding capacity (AWHC) and topographic variables varied between ecoregions and political jurisdictions and by fire season. AWHC explained more variability of fire ignitions in the Madrean Archipelago compared to the Chihuahuan Desert. Understanding the spatiotemporal distribution of recent fires in desert grasslands is needed to manage fire and predict responses to climate change. The use of landscape units such as ecological sites presents an opportunity to improve predictions at management scales. C1 [Levi, Matthew R.; Bestelmeyer, Brandon T.] New Mexico State Univ, USDA ARS Jornada Expt Range, MSC 3JER, Box 30003, Las Cruces, NM 88003 USA. RP Levi, MR (reprint author), New Mexico State Univ, USDA ARS Jornada Expt Range, MSC 3JER, Box 30003, Las Cruces, NM 88003 USA. EM mrlevi21@nmsu.edu FU USDA ARS Postdoctoral Research Associate Program; NSF Long-Term Ecological Research Program [DEB0080412] FX This work was supported by the USDA ARS Postdoctoral Research Associate Program and the NSF Long-Term Ecological Research Program (DEB0080412). We would like to acknowledge C. Stripling for sharing data from the Texas Forest Service database, S. Schrader for help with collecting and processing spatial data for environmental covariates, B. Cade for suggestions regarding model averaging and D. James for help with R code. NR 58 TC 0 Z9 0 U1 19 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD NOV PY 2016 VL 31 IS 9 BP 2079 EP 2095 DI 10.1007/s10980-016-0383-9 PG 17 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DX5RB UT WOS:000384438000012 ER PT J AU Prasad, AM Iverson, LR Matthews, SN Peters, MP AF Prasad, Anantha M. Iverson, Louis R. Matthews, Stephen N. Peters, Matthew P. TI A multistage decision support framework to guide tree species management under climate change via habitat suitability and colonization models, and a knowledge-based scoring system SO LANDSCAPE ECOLOGY LA English DT Article DE Decision support system; Tree habitat suitability model; Tree species migration model; Tree species distribution model; Forest management; Climate change ID EASTERN UNITED-STATES; RANGE SHIFTS; SEED DISPERSAL; GLOBAL CHANGE; POPULATION BIOLOGY; SCALE; DISTRIBUTIONS; UNCERTAINTY; CHALLENGES; FORESTS AB No single model can capture the complex species range dynamics under changing climates-hence the need for a combination approach that addresses management concerns. A multistage approach is illustrated to manage forested landscapes under climate change. We combine a tree species habitat model-DISTRIB II, a species colonization model-SHIFT, and knowledge-based scoring system-MODFACs, to illustrate a decision support framework. Using shortleaf pine (Pinus echinata) and sugar maple (Acer saccharum) as examples, we project suitable habitats under two future climate change scenarios (harsh, Hadley RCP8.5 and mild CCSM RCP4.5 at similar to 2100) at a resolution of 10 km and assess the colonization likelihood of the projected suitable habitats at a 1 km resolution; and score biological and disturbance factors for interpreting modeled outcomes. Shortleaf pine shows increased habitat northward by 2100, especially under the harsh scenario of climate change, and with higher possibility of natural migration confined to a narrow region close to the current species range boundary. Sugar maple shows decreased habitat and has negligible possibility of migration within the US due to a large portion of its range being north of the US border. Combination of suitable habitats with colonization likelihoods also allows for identification of potential locations appropriate for assisted migration, should that be deemed feasible. The combination of these multiple components using diverse approaches leads to tools and products that may help managers make management decisions in the face of a changing climate. C1 [Prasad, Anantha M.; Iverson, Louis R.; Peters, Matthew P.] USDA Forest Serv, Northern Res Stn, 359 Main Rd, Delaware, OH 43015 USA. [Matthews, Stephen N.] Ohio State Univ, Sch Environm & Nat Resources, 2021 Coffey Rd, Columbus, OH 43210 USA. RP Prasad, AM (reprint author), USDA Forest Serv, Northern Res Stn, 359 Main Rd, Delaware, OH 43015 USA. EM aprasad@fs.fed.us RI Matthews, Stephen/D-1050-2012 FU Northern Research Station, USDA Forest Service FX Thanks to the Northern Research Station, USDA Forest Service, for funding, and external reviewers Maria Janowiak and Laura Leites for their help in improving the manuscript. We declare no conflict of interest to the best of our knowledge. NR 71 TC 0 Z9 0 U1 19 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD NOV PY 2016 VL 31 IS 9 BP 2187 EP 2204 DI 10.1007/s10980-016-0369-7 PG 18 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DX5RB UT WOS:000384438000019 ER PT J AU Proestou, DA Vinyard, BT Corbett, RJ Piesz, J Allen, SK Small, JM Li, C Liu, M DeBrosse, G Guo, XM Rawson, P Gomez-Chiarri, M AF Proestou, Dina A. Vinyard, Bryan T. Corbett, Ryan J. Piesz, Jessica Allen, Standish K., Jr. Small, Jessica M. Li, Cui Liu, Ming DeBrosse, Gregory Guo, Ximing Rawson, Paul Gomez-Chiarri, Marta TI Performance of selectively-bred lines of eastern oyster, Crassostrea virginica, across eastern US estuaries SO AQUACULTURE LA English DT Article DE Crassostrea virginica; Performance; Selection; G x E interaction; Disease; Aquaculture; Oyster; Breeding ID HAPLOSPORIDIUM-NELSONI MSX; QUANTITATIVE PCR ASSAY; ENVIRONMENT INTERACTIONS; DISEASE RESISTANCE; PERKINSUS-MARINUS; DERMO DISEASE; DELAWARE BAY; MORTALITY; GROWTH; SURVIVAL AB Eastern oyster, Crassostrea virginica, aquaculture has expanded greatly in recent years, but further growth of the industry is constrained by disease-related losses. Oyster breeding programs supporting the oyster aquaculture industry along the east coast of the US have targeted resistance to three prominent diseases: MSX, Dermo, and ROD, caused by Haplosporidium nelsoni, Perkinsus marinus, and Roseovarius crassostreae respectively. Consequently, selected oyster lines possess some level of resistance and/or tolerance but the extent to which these lines, derived from various programs, perform across diverse growing environments used by industry has not been tested. The performance of six selected eastern oyster lines was evaluated at five sites along the east coast of the US (Maine to Virginia) to 1) identify differences in performance among lines at each site, and 2) identify lines that perform well across all sites. Performance measures included growth, mortality, and yield over a 15-month evaluation period. During unusually high mortality events, subsets of oysters were processed for disease diagnosis. Growth trajectories were similar among lines within a site, but varied significantly across sites (78% of random variance explained). Oysters grown in Rhode Island were largest while oysters grown in Maine were smallest at the end of the study. Mortality varied greatly among lines at each site as well as among sites. Line x site interaction explained 61% of the total random variance in the mortality data. In Maine, extensive mortality was observed early in the year for all lines, coincident with increased ROD prevalence. In New Jersey and Virginia, unusually high mortality was evident in the UMFS, Clinton, and NEH-RI lines during the final months of the experiment when the prevalence of both Dermo and MSX were 100% and <50%, respectively. NEH, DEBY, and hANA lines were less affected, demonstrating that lines selected to perform better in their native site surpassed those selected outside the area. Despite large and significant line x site interaction effects for mortality and yield, NEH, DEBY, and hANA performance was above average across all sites. These findings have important implications for oyster breeding strategies and industry practices. Statement of relevance: First to evaluate multiple oyster lines across diverse sites. Published by Elsevier B.V. C1 [Proestou, Dina A.] ARS, USDA, Natl Cold Water Marine Aquaculture Ctr, 469 CBLS,120 Flagg Rd, Kingston, RI 02881 USA. [Vinyard, Bryan T.] ARS, USDA, Northeast Area Stat Grp, Bldg 005,Room 126,10300 Baltimore Ave, Beltsville, MD 20705 USA. [Corbett, Ryan J.; Piesz, Jessica; Gomez-Chiarri, Marta] Dept Fisheries Anim & Vet Sci, 170 CBLS,120 Flagg Rd, Kingston, RI 02881 USA. [Allen, Standish K., Jr.; Small, Jessica M.] Coll William & Mary, Virginia Inst Marine Sci, Rt 1208 Greate Rd, Gloucester Point, VA 23062 USA. [Li, Cui; Liu, Ming; DeBrosse, Gregory; Guo, Ximing] Rutgers State Univ, Dept Marine & Coastal Sci, Haskin Shellfish Res Lab, 6959 Miller Ave, Port Norris, NJ 08349 USA. [Rawson, Paul] Univ Maine, 5751 Murray Hall, Orono, ME 04469 USA. RP Proestou, DA (reprint author), ARS, USDA, Natl Cold Water Marine Aquaculture Ctr, 469 CBLS,120 Flagg Rd, Kingston, RI 02881 USA. EM dina.proestou@ars.usda.gov; bryan.vinyard@ars.usda.gov; corbett214@uri.edu; piesz.jessica@gmail.com; ska@vims.edu; jamoss@vims.edu; cuili@hsrl.rutgers; mliu@hsrl.rutgers.edu; debrosse@rci.rutgers.edu; xguo@hsrl.rutgers.edu; prawson@maine.edu; gomezchi@uri.edu FU USDA ARS CRIS project [80303100000300D] FX The authors thank Chris Davis (Pequamid Oyster Co.), Russell Blank (Rome Point Oysters), and Rob Krause (Ninigret Oyster Farm) for providing space at their aquaculture leases in ME, RIBay, and RIPond. Additional assistance was provided by hatchery and field crews at the University of Maine and the Virginia Institute of Marine Science, and several undergraduate students at the University of Rhode Island. Funding for this project was obtained through the USDA ARS CRIS project # 80303100000300D. NR 56 TC 1 Z9 1 U1 20 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD NOV 1 PY 2016 VL 464 BP 17 EP 27 DI 10.1016/j.aquaculture.2016.06.012 PG 11 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA DW0YT UT WOS:000383370300003 ER PT J AU Evenhuis, JP Mohammed, H LaPatra, SE Welch, TJ Arias, CR AF Evenhuis, Jason P. Mohammed, Haitham LaPatra, Scott E. Welch, Timothy J. Arias, Covadonga R. TI Virulence and molecular variation of Flavobacterium columnare affecting rainbow trout in Idaho, USA SO AQUACULTURE LA English DT Article DE Flavobacterium columnare; Genomovar I; AFLP; Bacterial challenge ID CATFISH ICTALURUS-PUNCTATUS; FLEXIBACTER-COLUMNARIS; ONCORHYNCHUS-MYKISS; FISH; GENOMOVAR; ASSOCIATION AB Columnaris disease, caused by Flavobacterium columnare, is an emerging problem in the rainbow trout (Oncorhynchus mykiss) aquaculture industry of Idaho. All F. columnare isolates taken from disease outbreaks in the rain bow trout producing region of southern Idaho, and for rainbow trout, are all genomovar I. Virulence phenotyping of 11 genomovar I and 1 genomovar I/II isolates, taken from 6 different farms, suggests significant variation in virulence toward rainbow trout with the most virulent strains having an LD50 of 1 x 10(6) CFU/ml(-1) when using a waterborne challengemodel. The least virulent strain required 8 x 10(6) CFU/ml(-1) to reach the LD50. AFLP fingerprinting of these strains and the resulting phylogenetic tree show that all of the strains tested had a percent similarity of 75% or higher, save one, the MS-FC-4 strain had <60% identity to all of the other strains tested. These results suggest strains of F. columnare isolated from disease outbreaks in rainbow trout production have a higher degree of diversity than previously thought. Published by Elsevier B.V. C1 [Evenhuis, Jason P.; Welch, Timothy J.] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, 11861 Leetown Rd, Kearneysville, WV 25430 USA. [Mohammed, Haitham] Assiut Univ, Dept Anim Med, Fac Vet Med, Assiut 71526, Egypt. [LaPatra, Scott E.] Clear Springs Foods Inc, Div Res, Buhl, ID 83316 USA. [Arias, Covadonga R.] Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, 203 Swingle Hall, Auburn, AL 36849 USA. RP Evenhuis, JP (reprint author), USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, 11861 Leetown Rd, Kearneysville, WV 25430 USA. EM jason.evenhuis@ars.usda.gov FU USDA/ARS CRIS [8082-32000-006] FX The authors thank Ryan Lipscomb, Clayton Birkett, James Kincaid and Travis Moreland for expert technical assistance, and Carol A. Evenhuis for critical comments on the manuscript. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. This work was supported by USDA/ARS CRIS 8082-32000-006, USDA is an equal opportunity provider and employer. NR 23 TC 1 Z9 1 U1 6 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD NOV 1 PY 2016 VL 464 BP 106 EP 110 DI 10.1016/j.aquaculture.2016.06.017 PG 5 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA DW0YT UT WOS:000383370300014 ER PT J AU Schroeter, JC Peterson, BC Small, BC AF Schroeter, Julie C. Peterson, Brian C. Small, Brian C. TI Development of a multitissue microfluidic array for assessing changes in gene expression associated with channel catfish appetite, growth, metabolism, and intestinal health SO AQUACULTURE LA English DT Article DE Channel catfish; Gene expression; Array; Microfluidic ID COMPLEMENTARY DEOXYRIBONUCLEIC-ACID; ORANGE-SPOTTED GROUPER; FOOD-INTAKE; NEUROPEPTIDE-Y; MESSENGER-RNA; MOLECULAR CHARACTERIZATION; EPINEPHELUS-COIOIDES; ICTALURUS-PUNCTATUS; HORMONE-SECRETION; SPARUS-AURATA AB Large-scale, gene expression profiling methods allow for high throughput analysis of physiological pathways at a fraction of the cost of individual gene expression analysis. Systems, such as the Fluidigm quantitative PCR array described here, can provide powerful assessments of the effects of diet, environment, and management on physiological pathways affecting production parameters. A targeted microfluidic PCR array was designed and validated, for channel catfish (Ictalurus punctatus) representing key pathways involved in appetite, growth, metabolism, and intestinal inflammation for their potential to provide insight into the effects of diet and dietary supplements on these important physiological processes regulating feed efficiency and growth. With few exceptions, PCR primers were designed from Ictaluridae gene sequences published in GenBank. PCR amplicons from primers designed outside of Ictaluridae were sequenced to verify gene identity. All target gene primers were initially validated via conventional real-time qPCR (RT-qPCR). Combined hypothalamus/pituitary, hepatic, stomach, and intestinal tissue were used validate a 48.48 microfluidic PCR array to analyze multitissue gene expression. Use of the Fluidigm array resulted in reliable cycle threshold levels (Ct), efficiencies (E), and quality threshold scores (QS) for all but eight genes examined. Of the potential reference genes included in the panel, alpha-tubulin (TUBA) had a high QS, E, and acceptable Ct. The high throughput application of this technology, relative to conventional RT-qPCR, for assessing dietary effects on these pathways is demonstrated. Development of this targeted multi-tissue microfluidic array paves the way for the rapid evaluation of regulatory pathways in response to alternative feeding strategies, dietary formulations, and supplementation, as well as environmental and management effects for improving channel catfish culture and validates a cost-effective, dynamic, gene expression platform for use with other cultured fishes. (C) 2016 Elsevier B.V. All rights reserved. C1 [Schroeter, Julie C.] Southern Illinois Univ, Dept Anim Sci Food & Nutr, Ctr Fisheries Aquaculture & Aquat Sci, Carbondale, IL 62901 USA. [Small, Brian C.] Univ Idaho, Inst Aquaculture Res, Dept Fish & Wildlife Sci, Hagerman, ID 83332 USA. [Peterson, Brian C.] USDA ARS, Warmwater Aquaculture Res Unit, Stoneville, MS 38776 USA. RP Small, BC (reprint author), Univ Idaho, Inst Aquaculture Res, Dept Fish & Wildlife Sci, Hagerman, ID 83332 USA. EM bcsmall@uidaho.edu NR 45 TC 0 Z9 0 U1 11 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD NOV 1 PY 2016 VL 464 BP 213 EP 221 DI 10.1016/j.aquaculture.2016.06.036 PG 9 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA DW0YT UT WOS:000383370300030 ER PT J AU Anderson, AD Alam, MS Watanabe, WO Carroll, PM Wedegaertner, TC Dowd, MK AF Anderson, A. D. Alam, M. S. Watanabe, W. O. Carroll, P. M. Wedegaertner, T. C. Dowd, M. K. TI Full replacement of menhaden fish meal protein by low-gossypol cottonseed flour protein in the diet of juvenile black sea bass Centropristis striata SO AQUACULTURE LA English DT Article DE Black Sea bass; Gossypol; Cottonseed meal; Growth; Feed utilization; Tissue amino acids and fatty acids; Digestibility ID TROUT ONCORHYNCHUS-MYKISS; FATTY-ACID-COMPOSITION; SOYBEAN-MEAL; RAINBOW-TROUT; GLANDLESS COTTONSEED; CHANNEL CATFISH; SPARUS-AURATA; DIGESTIBILITY COEFFICIENTS; APPARENT DIGESTIBILITY; TRACHINOTUS-CAROLINUS AB Eight isonitrogenous (46% crude protein) and isolipidic (14% crude lipid) diets were formulated for juvenile black sea bass Centropristis striata to replace menhaden fish meal (FM) protein (59.5% CP) by three cottonseed meal (CSM) proteins: a CSM prepared from glandless seed (GCSM, 50.4% CP), a CSM that had been solvent extracted with acidic ethanol to remove the gossypol (SCSM, 53.8% CP), and a CSM prepared from regular (glanded) cottonseed (RCSM, 45% CP). Three diets replaced 50, 75 and 100% of FM protein with GCSM, and three diets replaced 50, 75 and 100% of FM protein with SCSM. One diet replaced 100% FM protein with RCSM protein. A control diet (0% CSM) was formulated with high FM protein and other practical protein sources. L-methionine and L-lysine were supplemented to the diets to equal the control diet. Fifteen fish were stocked in each of twenty-four 75-L tanks, and each test diet was fed to triplicate groups of fish (mean initial weight = 7.7 +/- 0.10 g) for 56 days at 22-24 degrees C, 32-35 salinity, and ambient photoperiod conditions. Fish were fed twice per day to apparent satiation. Final (day 56) mean body weight (BW, 24.16-31.62 g), body weight gain (BWG, 211.2-311.3%), specific growth rate (SGR, 1.70-2.18%/d), and feed intake (FI, 0.37-0.43 g/fish/d) were not significantly different (P > 0.05) among the control, GCSM or SCSM treatments, but were reduced (P < 0.05) for the 100% RCSM treatment (20.09 g, 159.5%, 1.45%/d, and 0.25 g/fish/d, respectively). Lower palatability of the RCSM diet was attributable to the anti-nutrient compound gossypol. No significant differences in survival (84.1-97.8%), feed conversion ratio (FCR, 1.05-1.33), protein efficiency ratio (PER, 1.62-2.08), or whole body protein or lipid composition were observed among the fish fed the low-gossypol diets. Gossypol (25.9 mg/kg) was only detectable in the livers of fish fed the high-gossypol RCSM diet. Fish whole body essential amino acid compositions did not differ significantly among treatments. Whole body n - 3 PUFAs decreased, while n-6 PUFAs increased with increasing CSM protein in the diets. The apparent digestibility coefficient of protein was high (83.1-87.1%) for all treatments. For juvenile black sea bass, 75% FM in the diet can be replaced with low-gossypol CSM protein prepared by solvent-extraction, and 100% of FM can be replaced with low-gossypol CSM protein prepared from glandless seed with no adverse effects on survival, growth or feed utilization. Statement of relevance: Cotton seed flour is an inexpensive agricultural by-product. The results demonstrate successful replacement of fish meal protein with genetically-improved cottonseed flour protein in the diet of black sea bass. These findings may substantially lower aquafeed costs (a key operational cost) for fin fish growout operations to boost profitability and accelerate expansion of commercial production of black sea bass and other fin-fish species. (C) 2016 Elsevier B.V. All rights reserved. C1 [Anderson, A. D.; Alam, M. S.; Watanabe, W. O.; Carroll, P. M.] Univ North Carolina Wilmington, Ctr Marine Sci, Aquaculture Program, 601 S Coll Rd, Wilmington, NC 28403 USA. [Wedegaertner, T. C.] Cotton Inc, 6399 Weston Pkwy, Cary, NC 27513 USA. [Dowd, M. K.] USDA ARS, New Orleans, LA USA. RP Watanabe, WO (reprint author), Univ North Carolina Wilmington, Ctr Marine Sci, Aquaculture Program, 601 S Coll Rd, Wilmington, NC 28403 USA. EM watanabew@uncw.edu FU Cotton Inc. [13-806] FX This research was supported by Cotton Inc. (Agreement No. 13-806). We thank Dr. Pamela J. Seaton for helpful advice and Macklin Corum, James Hill, Robert Farnell, Jen Gabel, Tyler Gibson, Casey Kelly and Matthew Dawson for technical assistance. NR 67 TC 0 Z9 0 U1 11 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD NOV 1 PY 2016 VL 464 BP 618 EP 628 DI 10.1016/j.aquaculture.2016.08.006 PG 11 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA DW0YT UT WOS:000383370300079 ER PT J AU Jones, VP Mills, NJ Brunner, JF Horton, DR Beers, EH Unruh, TR Shearer, PW Goldberger, JR Castagnoli, S Lehrer, N Milickzy, E Steffan, SA Amarasekare, KG Chambers, U Gadino, AN Gallardo, RK Jones, WE AF Jones, Vincent P. Mills, Nicholas J. Brunner, Jay F. Horton, David R. Beers, Elizabeth H. Unruh, Thomas R. Shearer, Peter W. Goldberger, Jessica R. Castagnoli, Steve Lehrer, Nadine Milickzy, Eugene Steffan, Shawn A. Amarasekare, Kaushalya G. Chambers, Ute Gadino, Angela N. Gallardo, R. Karina Jones, Wendy E. TI From planning to execution to the future: An overview of a concerted effort to enhance biological control in apple, pear, and walnut orchards in the western US SO BIOLOGICAL CONTROL LA English DT Editorial Material DE Conservation biological control; Plant volatiles; Pesticide effects on natural enemies; IPM decision-making; Economic analysis; Outreach ID INDUCED PLANT VOLATILES; BENEFICIAL INSECTS; FIELD-EVALUATION; ATTRACTANTS AB We embarked on a large project designed to help enhance biological control in apple, pear and walnut orchards in the western U.S., where management programs were in the midst of a transition from older organo-phosphate insecticides to mating disruption and newer reduced-risk insecticides. A "pesticide replacement therapy" approach resulted in unstable management programs with unpredictable outbreaks of spider mites and aphids. Our project was designed to provide growers and pest managers with information on the effects of newer pesticide chemistries on a suite of representative natural enemies in both the laboratory and field, potential of new monitoring tools using herbivore-induced plant volatiles and floral volatiles, phenology of the key natural enemy species, economic consequences of using an enhanced biological control program, and value of an outreach program to get project outcomes into the hands of decision-makers. We present an overview of both the successes and failures of the project and of new projects that have spun off from this project to further enhance biological control in our systems in the near future. (C) 2016 Elsevier Inc. All rights reserved. C1 [Jones, Vincent P.; Brunner, Jay F.; Beers, Elizabeth H.; Lehrer, Nadine; Steffan, Shawn A.; Chambers, Ute; Gadino, Angela N.; Jones, Wendy E.] Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. [Mills, Nicholas J.] Univ Calif Berkeley, Dept Environm Sci & Policy Management, Berkeley, CA 94720 USA. [Horton, David R.; Unruh, Thomas R.; Milickzy, Eugene] USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. [Shearer, Peter W.; Castagnoli, Steve; Amarasekare, Kaushalya G.] Oregon State Univ, Mid Columbia Agr Res & Extens Ctr, 3005 Expt Stn Dr, Hood River, OR 97031 USA. [Goldberger, Jessica R.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Gallardo, R. Karina] Washington State Univ, Puyallup Res & Extens Ctr, Sch Econ Sci, Puyallup, WA 98371 USA. [Steffan, Shawn A.] Univ Wisconsin, Vegetable Crops Res Unit, USDA ARS, 1575 Linden Dr, Madison, WI 53706 USA. [Lehrer, Nadine] Chatham Univ, Sch Sustainabil, Pittsburgh, PA 15232 USA. [Amarasekare, Kaushalya G.] Tennessee State Univ, Dept Agr & Environm Sci, Room 202P,Farrell Westbrook Hall, Nashville, TN 37209 USA. RP Jones, VP (reprint author), Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. EM vpjones@wsu.edu NR 30 TC 4 Z9 4 U1 38 U2 38 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD NOV PY 2016 VL 102 BP 1 EP 6 DI 10.1016/j.biocontrol.2016.03.013 PG 6 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DW8XG UT WOS:000383938200001 ER PT J AU Beers, EH Horton, DR Miliczky, E AF Beers, Elizabeth H. Horton, David R. Miliczky, Eugene TI Pesticides used against Cydia pomonella disrupt biological control of secondary pests of apple SO BIOLOGICAL CONTROL LA English DT Article DE Eriosoma lanigerum; Aphelinus mali; Syrphidae; Neuroptera ID ERIOSOMA-LANIGERUM HAUSMANN; APHID HEMIPTERA APHIDIDAE; OCCIDENTALIS ACARI PHYTOSEIIDAE; REDUCED-RISK INSECTICIDES; INDUCED PLANT VOLATILES; GALENDROMUS-OCCIDENTALIS; ORCHARD PESTICIDES; BENEFICIAL INSECTS; WASHINGTON-STATE; SYNTHETIC PYRETHROIDS AB The effects of codling moth (Cydia pomonella (Linnaeus)) (Lepidoptera: Tortricidae) management programs on secondary pests of apple were examined from 2008 to 2011 in five replicated large-plot trials. The orchards were chosen for a history of Eriosoma lanigerum (Hausmann) (Hemiptera: Aphididae) and tetranychid mite outbreaks. Programs covered the first, second, or both generations of C. pomonella, and employed some of the newer insecticides for comparison to older materials. Increased levels of E. lanigerum were consistently associated with the use of spinetoram and novaluron throughout the tests. The mechanism of disruption was not completely clear, but suppression of Forficula auricularia Linnaeus (Dermaptera: Forficulidae) and Aphelinus mali (Haldeman) (Hymenoptera: Aphelinidae) were associated with the use of these materials. Methoxyfenozide, chlorantraniliprole, and azinphos-methyl did not disrupt biological control of tetranychid mites or aphids, and negative effects on natural enemies were infrequent. Lambda-cyhalothrin provided direct control of aphid species, thus reduced levels of natural enemies could also be ascribed to lack of prey. The effect of some of the insecticide applications made during the first generation were evident for many months after the application in terms of pest and natural enemy populations; however, attempts to mitigate disruptive effects by shifting the use of materials to the second generation of C. pomonella were not successful. The effect on biological control of tetranychid mites was less consistent, although some detrimental effects (increased pest or decreased predatory mite levels) were associated with spinetoram, novaluron, and lambda-cyhalothrin. The difficulties in sampling natural enemies and determining their impact made pest outbreaks the most reliable indicator of pesticide-induced disruption.(C) 2016 Elsevier Inc. All rights reserved. C1 [Beers, Elizabeth H.] Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. [Horton, David R.; Miliczky, Eugene] USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. RP Beers, EH (reprint author), Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. EM ebeers@wsu.edu; david.horton@ars.usda.gov; Gene.Miliczky@ars.usda.gov FU USDA Specialty Crop Research Initiative [2008-04854]; Washington Tree Fruit Research Commission [CP08-805] FX The authors gratefully acknowledge the technical help of Peter Smytheman, Bruce Greenfield, Allie Bock, Alix Whitener, Kaitlin Parsons, Natasza Lorentz, Kylie Martin, Alyssa White, Denise Burnett, Mattie Warner and David Gutierrez in pest and natural enemy sampling. These experiments were funded in part by USDA Specialty Crop Research Initiative Proposal Number 2008-04854 and the Washington Tree Fruit Research Commission (#CP08-805). NR 53 TC 4 Z9 4 U1 24 U2 24 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD NOV PY 2016 VL 102 BP 35 EP 43 DI 10.1016/1biocontrol.2016.05.009 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DW8XG UT WOS:000383938200005 ER PT J AU Beers, EH Mills, NJ Shearer, PW Horton, DR Milickzy, ER Amarasekare, KG Gontijo, LM AF Beers, Elizabeth H. Mills, Nicholas J. Shearer, Peter W. Horton, David R. Milickzy, Eugene R. Amarasekare, Kaushalya G. Gontijo, Lessando M. TI Nontarget effects of orchard pesticides on natural enemies: Lessons from the field and laboratory SO BIOLOGICAL CONTROL LA English DT Article DE Nontarget effects; Biological control; Tetranychidae; Aphididae; Pear psylla ID WORKING GROUP PESTICIDES; BENEFICIAL ORGANISMS; TESTING PROGRAM; BIOLOGICAL-CONTROL; APPLE; INSECTICIDES; TOXICITY; INSECTS; ARTHROPODS; HEMIPTERA AB The nontarget effects of insecticide programs used to control codling moth, Cydia pomonella L. (Lepidoptera: Tortricidae), were studied in large-plot field trials in apples, pears, and walnuts in the western United States. We assessed the health of the natural enemy community by sampling the abundance of natural enemies and by monitoring for outbreaks of secondary pests. The insecticides used in the field tests overlapped those tested in laboratory bioassays. Using these parallel lab and field studies, we examined two hypotheses: 1) pesticides found to have negative effects on natural enemy fitness in laboratory bioassays will predict reductions in natural enemy densities in the field, and 2) reductions in natural enemy densities in the field will result in outbreaks of secondary pests. We found only one clear instance, Forficula auricularia (L.) (Dermaptera: Forficulidae), where laboratory results documenting negative effects corresponded to a significant reduction in field studies (apple). This same instance was the only case where a reduction in a natural enemy population was associated with a significantly increased density of a secondary pest, Eriosoma lanigerum (Hausmann) (Hemiptera: Aphididae). There were several instances where secondary pest outbreaks were associated with unchanged or even increased natural enemy densities. The limited number of field trials, variability in field trial conditions among years and sites, duration of the negative effect relative to sampling interval, sampling efficiency, plot size/inter-plot movement, and compensation by other natural enemies attracted to a large host/prey resource may all have contributed to the poor predictive success. Overall, the laboratory bioassays predicted a far greater negative impact than was found in the field trials. (C) 2016 Elsevier Inc. All rights reserved. C1 [Beers, Elizabeth H.; Gontijo, Lessando M.] Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. [Mills, Nicholas J.] Univ Calif Berkeley, Dept Environm Sci & Policy Management, Berkeley, CA 94720 USA. [Shearer, Peter W.; Amarasekare, Kaushalya G.] Oregon State Univ, Mid Columbia Agr Res & Extens Ctr, 3005 Expt Stn Dr, Hood River, OR 97031 USA. [Horton, David R.; Milickzy, Eugene R.] USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. [Shearer, Peter W.] 1523 Lincoln St Unit 200, Hood River, OR 97031 USA. [Amarasekare, Kaushalya G.] Tennessee State Univ, Dept Agr & Environm Sci, Room 202 P Farrell Westbrook Hall, Nashville, TN 37209 USA. [Gontijo, Lessando M.] Univ Fed Vicosa, Campus Florestal, BR-35690000 Florestal, MG, Brazil. RP Beers, EH (reprint author), Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. EM ebeers@wsu.edu; nmills@berkeley.edu; shearpw@gmail.com; david.horton@ars.usda.gov; kamarase@tnstate.edu; lessandomg@gmail.com FU USDA Specialty Crop Research Initiative [2008-04854]; Washington Tree Fruit Research Commission FX The authors gratefully acknowledge the technical help of Peter Smytheman and Bruce Greenfield, as well as undergraduates Allie Bock, Alix Crilly, Kaitlin Parsons, Natasza Lorentz, Kylie Martin, Alyssa White, Denise Burnett, Mattie Warner and David Gutierrez in pest and natural enemy sampling. We thank Chris Sater for assistance in proofreading and graphics preparation. These experiments were funded in part by USDA Specialty Crop Research Initiative Proposal Number 2008-04854 and the Washington Tree Fruit Research Commission. NR 41 TC 8 Z9 8 U1 15 U2 15 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD NOV PY 2016 VL 102 BP 44 EP 52 DI 10.1016/j.biocontrol.2016.04.010 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DW8XG UT WOS:000383938200006 ER PT J AU Jones, VP Horton, DR Mills, NJ Unruh, TR Baker, CC Melton, TD Milickzy, E Steffan, SA Shearer, PW Amarasekare, KG AF Jones, Vincent P. Horton, David R. Mills, Nicholas J. Unruh, Thomas R. Baker, Callie C. Melton, Tawnee D. Milickzy, Eugene Steffan, Shawn A. Shearer, Peter W. Amarasekare, Kaushalya G. TI Evaluating plant volatiles for monitoring natural enemies in apple, pear and walnut orchards SO BIOLOGICAL CONTROL LA English DT Article DE Herbivore induced plant volatiles; Floral volatiles; Natural enemy monitoring ID IPM DECISION-MAKING; BENEFICIAL INSECTS; METHYL SALICYLATE; GREEN LACEWINGS; SAMPLING PLANS; CARNIVOROUS ARTHROPODS; BIOLOGICAL-CONTROL; FIELD-EVALUATION; CHRYSOPERLA; CHRYSOPIDAE AB The ability to estimate natural enemy abundance is crucial to the integration of biological control into IPM programs. Traditional sampling approaches for natural enemies are few and most are inefficient, but recent studies suggest attraction of natural enemies to plant volatiles may be a useful proxy for direct sampling. We evaluated various combinations of herbivore-induced plant volatiles and floral volatiles as monitoring tools for natural enemies found in apple, pear, and walnut orchards in California, Oregon, and Washington. In 2010 we used a full factorial experimental designs to evaluate lures for all combinations of acetic acid (AA), acetophenone (AP), phenylacetaldehyde (PAA) and 2-phenylethanol (PE). Of nine natural enemy taxa analyzed, we found syrphid flies responded strongly to PE, but combining AA with PE attenuated trap catch and combining PAA to PE eliminated the activity of PE. Chlysoperla spp. (Chrysopidae) responded strongly to most of the individual compounds and the various interactions between the components allowed multiple ways to achieve roughly the same trap catch. All of the hymenopteran taxa collected responded strongly to PAA, and PAA containing lures were nearly always a component of the top eight lures. A smaller factorial experiment testing all possible combinations of AA, PAA and methyl salicylate (MS) showed that single component AA or MS lures were generally not significantly different than the controls for all taxa tested, but for the hymenopteran taxa, traps baited with MS+PAA performed the best or were not significantly different than the best performing lure. A 2011 trial was conducted to test the influence of the addition of AA and/or MS on previously tested lures. Combining AA or MS with other lures, improved the capture of Chrysoperla spp.; Scaeva pyrastri (L.) (Syrphidae) capture was enhanced when MS was used with PE; and PE was attractive to the three syrphid flies, anysoperla spp., and the parasitoid Aphelinus mall (Haldeman) (Aphelinidae). The differential responses to various blends exhibited among taxa show that combinations of plant volatiles can be chosen to increase specificity of attraction to a few taxa or increase the number of species attracted. This flexibility should add to the general value and breadth of use of plant volatile monitoring lures. (C) 2015 Elsevier Inc. All rights reserved. C1 [Jones, Vincent P.; Baker, Callie C.; Melton, Tawnee D.; Steffan, Shawn A.] Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. [Horton, David R.; Unruh, Thomas R.; Milickzy, Eugene] USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. [Mills, Nicholas J.] Univ Calif Berkeley, Dept Environm Sci & Policy Management, Berkeley, CA 94720 USA. [Shearer, Peter W.; Amarasekare, Kaushalya G.] Oregon State Univ, Mid Columbia Agr Res & Extens Ctr, 3005 Expt Stn Dr, Hood River, OR 97031 USA. RP Jones, VP (reprint author), Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. EM vpjones@wsu.edu FU USDA-Specialty Crop Research Initiative [2008-04854]; Washington Tree Fruit Research Commission; California Walnut Board FX This work was supported in part by USDA-Specialty Crop Research Initiative grant 2008-04854, and grants from the Washington Tree Fruit Research Commission (to VPJ), California Walnut Board (to NJM). We thank Merilee Bayer, Deb Broers, Preston Brown, Lisa Fernandez, Aviva Goldmann, Kodi Jaspers, Laura Jones, Kevi Mace-Hill, Stacey McDonald, Francisco De La Rosa, Teah Smith, for technical support. NR 50 TC 7 Z9 7 U1 31 U2 31 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD NOV PY 2016 VL 102 BP 53 EP 65 DI 10.1016/j.biocontrol.2015.03.009 PG 13 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DW8XG UT WOS:000383938200007 ER PT J AU Mills, NJ Jones, VP Baker, CC Melton, TD Steffan, SA Unruh, TR Horton, DR Shearer, PW Amarasekare, KG Milickzy, E AF Mills, Nicholas J. Jones, Vincent P. Baker, Callie C. Melton, Tawnee D. Steffan, Shawn A. Unruh, Thomas R. Horton, David R. Shearer, Peter W. Amarasekare, Kaushalya G. Milickzy, Eugene TI Using plant volatile traps to estimate the diversity of natural enemy communities in orchard ecosystems SO BIOLOGICAL CONTROL LA English DT Article DE Apple; Evenness; Parasitoid; Pear; Predator; Richness; Walnut ID CONSERVATION BIOLOGICAL-CONTROL; ZEALAND APPLE ORCHARDS; PEST-MANAGEMENT; CARABIDS COLEOPTERA; BIODIVERSITY; EVENNESS; ARTHROPODS; PREDATORS; SERVICES; IMPACT AB In this study we used sticky traps baited with plant volatile lures to monitor the biodiversity of natural enemies in orchard ecosystems in the western U.S. We compared the diversity of predator genera from season total trap catches in 37 different orchards (apple, cherry, pear and walnut) over a two-year period (2010-2011) using standardized Hill number biodiversity indices and community similarity profiles. For a subset of 23 of these orchards we were also able to monitor the change in biodiversity of predator genera over the full growing season in the different orchard crops. A total of 37,854 individuals from 31 different genera of foliage-active generalist predators were collected from all orchards combined. Mean sample coverage was high (0.98) and richness, diversity and evenness differed between crops in 2010, but not in 2011. There was more than 90% similarity in the richness of predator genera among crops and among orchards within crops, but a greater level of differentiation was observed among orchards when variation in their relative abundance and dominance in the communities was taken into account. There was a consistent rise in predator generic richness and diversity through the season in both years for apple, cherry and pear orchards, but in walnut orchards, a steep rise from March to May was followed by a decline through the rest of the season. In an additional component of the study, the species level similarity of predator and parasitoid communities was analyzed for total season trap catch data from six walnut orchards. The rarefied species richness of parasitoids was much greater than that for predators, although the diversity, evenness and dominance of the parasitoid species varied considerably among orchards. The results from this study highlight the fact that natural enemy communities in orchard ecosystems can be effectively monitored using plant volatile traps, and that these communities are surprisingly diverse despite frequent disturbance from pest management intervention. (C) 2016 Elsevier Inc. All rights reserved. C1 [Mills, Nicholas J.] Univ Calif Berkeley, Dept Environm Sci & Policy Management, Berkeley, CA 94720 USA. [Jones, Vincent P.; Baker, Callie C.; Melton, Tawnee D.; Steffan, Shawn A.] Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. [Unruh, Thomas R.; Horton, David R.; Milickzy, Eugene] USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. [Shearer, Peter W.; Amarasekare, Kaushalya G.] Oregon State Univ, Mid Columbia Agr Res & Extens Ctr, 3005 Expt Stn Dr, Hood River, OR 97031 USA. [Amarasekare, Kaushalya G.] Tennessee State Univ, Dept Agr & Environm Sci, Room 202P,Farrell Westbrook Hall, Nashville, TN 37209 USA. RP Mills, NJ (reprint author), Univ Calif Berkeley, Dept Environm Sci & Policy Management, Berkeley, CA 94720 USA. EM nmills@berkeley.edu FU USDA-SCRI grant [2008-04854]; Washington Tree Fruit Research Commission; California Walnut Board; pear growers in Hood River (Oregon) FX This research was supported by USDA-SCRI grant 2008-04854 and matching funds from the Washington Tree Fruit Research Commission, California Walnut Board and pear growers in Hood River (Oregon). The research personnel and technical support of the laboratories from the University of California at Berkeley, Oregon State University, Washington State University and USDA-ARS Yakima are gratefully acknowledged. NR 70 TC 2 Z9 2 U1 20 U2 20 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD NOV PY 2016 VL 102 BP 66 EP 76 DI 10.1016/j.biocontrol.2016.05.001 PG 11 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DW8XG UT WOS:000383938200008 ER PT J AU Jones, VP Horton, DR Mills, NJ Unruh, TR Miliczky, E Shearer, PW Amarasekare, KG Baker, CC Melton, TD AF Jones, Vincent P. Horton, David R. Mills, Nicholas J. Unruh, Thomas R. Miliczky, Eugene Shearer, Peter W. Amarasekare, Kaushalya G. Baker, Callie C. Melton, Tawnee D. TI Using plant volatile traps to develop phenology models for natural enemies: An example using Chrysopa nigricornis (Burmeister) (Neuroptera: Chrysopidae) SO BIOLOGICAL CONTROL LA English DT Article DE Chrysopa nigricornis; Phenology model; Herbivore-induced plant volatiles; Apple; Walnut; Pear; Sweet cherry ID CODLING MOTH LEPIDOPTERA; BENEFICIAL INSECTS; METHYL SALICYLATE; CENTRAL WASHINGTON; FIELD-EVALUATION; PEST-MANAGEMENT; ATTRACTANTS; TEMPERATURE; PREDATORS; LACEWINGS AB A model predicting phenology of adult Chrysopa nigricornis (Burmeister) (Neuroptera: Chrysopidae) in orchards was developed from field (trapping) data supplemented with development data collected under laboratory conditions. Lower and upper thresholds of 10.1 degrees C and 29.9 degrees C, respectively, were estimated from published and unpublished laboratory observations, and were used to develop a phenology model. Season-long field data were collected using white delta traps that had been baited with squalene, a volatile shown previously to be highly attractive to C. nigricornis. The model was developed from data collected in three Washington apple orchards, and was validated using independent data sets collected from apple, sweet cherry, pear, and walnut orchards over a 2-4 year period across a much wider geographic region. We found that the mean absolute deviation across all crops and years was 39.7 +/- 1.2 day-degrees (DD), or 4.4 +/- 0.14 days. Populations of C nigricornis from walnut orchards in California emerged 105 DD later than those in Oregon and Washington, thus requiring correction of average time of first trap catch in California to synchronize models. The ability to use a single model across multiple crops, different prey species and abundances, and different pesticide regimes demonstrates that phenology models for generalist predators may have broader application to IPM programs in other cropping systems. (C) 2015 Elsevier Inc. All rights reserved. C1 [Jones, Vincent P.; Baker, Callie C.; Melton, Tawnee D.] Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. [Horton, David R.; Unruh, Thomas R.; Miliczky, Eugene] USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. [Mills, Nicholas J.] Univ Calif Berkeley, Dept Environm Sci & Policy Management, Berkeley, CA 94720 USA. [Shearer, Peter W.] Oregon State Univ, Mid Columbia Agr Res & Extens Ctr, 3005 Expt Stn Dr, Hood River, OR 97031 USA. [Amarasekare, Kaushalya G.] Tennessee State Univ, Dept Agr & Environm Sci, Room 202 P Farrell Westbrook Hall, Nashville, TN 37209 USA. RP Jones, VP (reprint author), Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. EM vpjones@wsu.edu; kamarase@tnstate.edu FU USDA-Specialty Crop Research Initiative [2008-04854]; Washington Tree Fruit Research Commission; California Walnut Board FX This work was supported in part by USDA-Specialty Crop Research Initiative Grant 2008-04854, and Grants from the Washington Tree Fruit Research Commission (to VPJ), California Walnut Board (to NJM). We thank Merilee Bayer, Deb Broers, Preston Brown, Lisa Fernandez, Aviva Goldmann, Kodi Jaspers, Laura Jones, Kevi Mace-Hill, Stacey McDonald, Francisco De La Rosa, Teah Smith, and Amanda Borel for technical support. NR 47 TC 5 Z9 5 U1 9 U2 9 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD NOV PY 2016 VL 102 BP 77 EP 84 DI 10.1016/j.biocontrol.2014.12.012 PG 8 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DW8XG UT WOS:000383938200009 ER PT J AU Unruh, TR Miliczky, ER Horton, DR Thomsen-Archer, K Rehfield-Ray, L Jones, VP AF Unruh, Thomas R. Miliczky, Eugene R. Horton, David R. Thomsen-Archer, Kelly Rehfield-Ray, Linda Jones, Vincent P. TI Gut content analysis of arthropod predators of codling moth in Washington apple orchards SO BIOLOGICAL CONTROL LA English DT Article DE Cydia pomonella; Predatory arthropods; Polymerase chain reaction; Conservation biological control ID POLYMERASE-CHAIN-REACTION; DETECTABILITY HALF-LIFE; COLORADO POTATO BEETLE; GENERALIST PREDATORS; BIOLOGICAL-CONTROL; CYDIA-POMONELLA; DIRECT-PCR; FORFICULA-AURICULARIA; LACANOBIA-SUBJUNCTA; MOLECULAR ANALYSIS AB Codling moth, Cydia pomonella (L.), is the key pest of pome fruits in many temperate areas of North America, Eurasia, South Africa, South America and Australia. Many predatory arthropods species are found in organic apple orchards of central Washington; here we use PCR-based gut content analysis of arthropod predators to identify predators that attack codling moth. Predators were sampled from tree canopies, tree trunks and from the understory and were homogenized in a lysis buffer to provide a template for Direct PCR. PCR showed 8.9% of 2591 predators had preyed on C. pomonella. Spiders, including 25 genera from 15 families, two carabid beetle species (Pterostichus melanarius (Illiger) and Harpalus penn-sylvanicus DeGeer) and the European earwig (Forficula auricularia [L.]) represented 87% of predator specimens analyzed and were 8.2%, 8.3% and 14.7% positive for C pomonella. PCR products from 38% of predators that appeared positive for C. pomonella COI were sequenced; all showed 99% or more similarity to C. pomonella COI sequences in GenBank. Digestion rates of adult earwigs fed on mature codling moth larvae showed a detection half-life of 3.7 days; half-life from the fecal pellets from the same earwigs was 3.6 days. When fed mature codling moth larvae, the carabid P. melanarius showed a digestion half-life of 3.14 days. Identification of the key predators of C. pomonella can guide the use of selective insecticides and the conservation of these natural enemies, enhancing biological control and supporting stable IPM programs in pome fruit orchards in the western USA. Published by Elsevier Inc. C1 [Unruh, Thomas R.; Miliczky, Eugene R.; Horton, David R.; Thomsen-Archer, Kelly; Rehfield-Ray, Linda] USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. [Jones, Vincent P.] Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. RP Unruh, TR (reprint author), USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM thomas.unruh@ars.usda.gov FU Washington Tree Fruit Research Commission; USDA Specialty Crop Research Initiative FX This manuscript was greatly improved by editorial and analytical suggestions provided by Nicholas Mills of the University of California at Berkeley. Editorial suggestions by James Hagler, USDA, ARS, Maricopa AZ and Marshal W.Johnson, University of California, Riverside, emeritus, and three anonymous reviewers also greatly helped improve the manuscript. Funding by the Washington Tree Fruit Research Commission and the USDA Specialty Crop Research Initiative made the study possible. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 84 TC 2 Z9 2 U1 29 U2 29 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD NOV PY 2016 VL 102 BP 85 EP 92 DI 10.1016/j.biocontrol.2016.05.014 PG 8 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DW8XG UT WOS:000383938200010 ER PT J AU Marwarha, G Claycombe, K Schommer, J Collins, D Ghribi, O AF Marwarha, Gurdeep Claycombe, Kate Schommer, Jared Collins, David Ghribi, Othman TI Palmitate-induced Endoplasmic Reticulum stress and subsequent C/EBP alpha Homologous Protein activation attenuates leptin and Insulin-like growth factor 1 expression in the brain SO CELLULAR SIGNALLING LA English DT Article DE Endoplasmic Reticulum stress; Leptin; Insulin-like growth factor 1; Palmitic acid; CCAAT Enhancer Binding Protein alpha; C/EBP Homologous Protein ID ADULT HIPPOCAMPAL NEUROGENESIS; DOMINANT-NEGATIVE INHIBITOR; DIETARY FATTY-ACIDS; ALZHEIMERS-DISEASE; FACTOR-I; HUNTINGTONS-DISEASE; TAU PHOSPHORYLATION; MITOCHONDRIAL-FUNCTION; TRANSCRIPTION FACTORS; PARKINSONS-DISEASE AB The peptide hormones Insulin-like growth factor-1 (IGF1) and leptin mediate a myriad of biological effects - both in the peripheral and central nervous systems. The transcription of these two hormones is regulated by the transcription factor C/EBP alpha, which in turn is negatively regulated by the transcription factor C/EBP Homologous Protein (CHOP), a specific marker of endoplasmic reticulum (ER) stress. In the peripheral system, disturbances in leptin and IGF-1 levels are implicated in a variety of metabolic diseases including obesity, diabetes, atherosclerosis and cardiovascular diseases. Current research suggests a positive correlation between consumption of diets rich in saturated free fatty acids (sFFA) and metabolic diseases. Induction of ER stress and subsequent dysregulation in the expression levels of leptin and IGF-1 have been shown to mediate sFFA-induced metabolic diseases in the peripheral system. Palmitic acid (palmitate), the most commonly consumed sFFA, has been shown to be up taken by the brain, where it may promote neurodegeneration. However, the extent to which palmitate induces ER stress in the brain and attenuates leptin and IGF1 expression has not been determined. We fed C57BL/6J mice a palmitate-enriched diet and determined effects on the expression levels of leptin and IGF1 in the hippo campus and cortex. We further determined the extent to which ER stress and subsequent CHOP activation mediate the palmitate effects on the transcription of leptin and IGF1. We demonstrate that palmitate induces ER stress and decreases leptin and IGF1 expression by inducing the expression of CHOP. The molecular chaperone 4-phenylbutyric acid (4-PBA), an inhibitor of ER stress, precludes the palmitate-evoked down-regulation of leptin and IGF1 expression. Furthermore, the activation of CHOP in response to ER stress is pivotal in the attenuation of leptin and IGF1 expression as knocking-down CHOP in mice or in SH-SY5Y and Neuro-2a (N2a) cells rescues the palmitate-induced mitigation in leptin and IGF1 expression. Our study implicates for the first time ER stress-induced CHOP activation in the brain as a mechanistic link in the palmitate-induced negative regulation of leptin and IGF1, two neurotrophic cytokines that play an indispensable role in the mammalian brain. (C) 2016 Elsevier Inc. All rights reserved. C1 [Marwarha, Gurdeep; Schommer, Jared; Collins, David; Ghribi, Othman] Univ North Dakota, Sch Med & Hlth Sci, Dept Biomed Sci, Grand Forks, ND 58203 USA. [Claycombe, Kate] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58203 USA. RP Ghribi, O (reprint author), Univ North Dakota, Dept Biomed Sci, Sch Med & Hlth Sci, 501 North Columbia Rd, Grand Forks, ND 58202 USA. EM othman.ghribi@med.und.edu OI MARWARHA, GURDEEP/0000-0001-5556-0003 FU National Institutes of Health [R01AG0145264] FX This work was supported by National Institutes of Health, Grant # R01AG0145264, to Dr. Othman Ghribi. NR 70 TC 0 Z9 0 U1 12 U2 12 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0898-6568 EI 1873-3913 J9 CELL SIGNAL JI Cell. Signal. PD NOV PY 2016 VL 28 IS 11 BP 1789 EP 1805 DI 10.1016/j.cellsig.2016.08.012 PG 17 WC Cell Biology SC Cell Biology GA DW8YU UT WOS:000383942200018 PM 27555288 ER PT J AU Kim, S Williams, A Kiniry, JR Hawkes, CV AF Kim, Sumin Williams, Amber Kiniry, James R. Hawkes, Christine V. TI Simulating diverse native C-4 perennial grasses with varying rainfall SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Forage; Rainfall; ALMANAC; Plant growth; Perennial grass ID PLANT-COMMUNITIES; NORTH-AMERICAN; WATER-USE; PRECIPITATION; PRODUCTIVITY; SWITCHGRASS; EFFICIENCY; RESPONSES; LIVESTOCK; BIOMASS AB Rainfall is recognized as a major factor affecting the rate of plant growth development. The impact of changes in amount and variability of rainfall on growth and production of different forage grasses needs to be quantified to determine how climate change can impact rangelands. Comparative studies to evaluate the growth of several perennial forage species at different rainfall rates will provide useful information by identifying forage management strategies under various rainfall scenarios. In this study, the combination of rainfall changes and soil types on the plant growth of 10 perennial forage species was investigated with both the experimental methods, using rainout shelters, and with the numerical methods using the plant growth simulation model, ALMANAC. Overall, most species significantly increased basal diameter and height as rainfall increased. Like measured volume, simulated yields for all species generally increased as rainfall increased. But, large volume and yield increases were only observed between 350 and 850 mm/yr. Simulating all species growing together competing agrees relatively well with observed plant volumes at low rainfall treatment, while simulating all species growing separately was slightly biased towards overestimation on low rainfall effect. Both simulations agree relatively well with observed plant volume at high rainfall treatment. Published by Elsevier Ltd. This is an open access article under the CC BY license. C1 [Kim, Sumin] Oak Ridge Inst Sci & Educ, Oak Ridge, TN 37831 USA. [Williams, Amber; Kiniry, James R.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Hawkes, Christine V.] Univ Texas Austin, Sect Integrat Biol, Austin, TX 78712 USA. RP Kiniry, JR (reprint author), USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA.; Hawkes, CV (reprint author), Univ Texas Austin, Sect Integrat Biol, Austin, TX 78712 USA. EM Jim.Kiniry@ARS.USDA.GOV; chawkes@austin.utexas.edu OI Williams, Amber/0000-0001-6898-8543 FU Department of Energy (DOE) [08-SC-NICCR-1071]; Interagency Reimbursable Agreement [60-3098-5-006]; U.S. Department of Energy; U.S. Department of Agriculture, Agricultural Research Service Agreement [60-3098-5-002] FX We are grateful to Nick Johnson, Elise Worchel, Hannah Giauque, Lauren Taranow, Gabe Miller, and other members of the Hawkes lab who assisted with data collection. We also thank Stephanie Silvia, Civil Engineering department at University of Texas at San Antonio, who did some of the preliminary analyses. Funding for the shelter experiment was provided by the Department of Energy (DOE) #08-SC-NICCR-1071 to CVH. This work was funded by Interagency Reimbursable Agreement #60-3098-5-006. This work was also supported in part by an appointment to Agricultural Research Service administrated by Oak Ridge Institute for Science and Education through interagency agreement between U.S. Department of Energy and U.S. Department of Agriculture, Agricultural Research Service Agreement #60-3098-5-002. NR 50 TC 0 Z9 0 U1 6 U2 6 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 EI 1095-922X J9 J ARID ENVIRON JI J. Arid. Environ. PD NOV PY 2016 VL 134 BP 97 EP 103 DI 10.1016/j.jaridenv.2016.07.004 PG 7 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DW8WH UT WOS:000383935700013 ER PT J AU Edwin, ES Vasantha-Srinivasan, P Senthil-Nathan, S Thanigaivel, A Ponsankar, A Pradeepa, V Selin-Rani, S Kalaivani, K Hunter, WB Abdel-Megeed, A Duraipandiyan, V Al-Dhabi, NA AF Edwin, Edward-Sam Vasantha-Srinivasan, Prabhakaran Senthil-Nathan, Sengottayan Thanigaivel, Annamalai Ponsankar, Athirstam Pradeepa, Venkatraman Selin-Rani, Selvaraj Kalaivani, Kandaswamy Hunter, Wayne B. Abdel-Megeed, Ahmed Duraipandiyan, Veeramuthu Al-Dhabi, Naif Abdullah TI Anti-dengue efficacy of bioactive andrographolide from Andrographis paniculata (Lamiales: Acanthaceae) against the primary dengue vector Aedes aegypti (Diptera: Culicidae) SO ACTA TROPICA LA English DT Article DE Dengue; Bioactive; RP-HPLC; Repellent; Mortality; Enzyme; Histology ID CULEX-QUINQUEFASCIATUS SAY.; ESSENTIAL OILS; ENZYME-ACTIVITIES; IN-VITRO; MOSQUITO; EXTRACT; PLANTS; LARVAE; INSECTICIDE; REPELLENT AB The current study investigated the toxic effect of the leaf extract compound andrographolide from Andrographis paniculata (Burm.f) against the dengue vector Ae. aegypti. GC-MS analysis revealed that andrographolide was recognized as the major chemical constituent with the prominent peak area compared with other compounds. All isolated toxic compounds were purified and confirmed through RP-HPLC against chemical standards. The larvicidal assays established at 25 ppm of bioactive compound against the treated instars of Ae. Aegypti showed prominent mortality compared to other treated concentrations. The percent mortality of larvae was directly proportional to concentration. The lethal concentration (LC50) was observed at 12 ppm treatment concentration. The bioactive andrographolide considerably reduced the detoxifying enzyme regulations of alpha- and beta- carboxylesterases. In contrast, the levels of GST and CYP450 significantly increase in a dose dependent manner. The andrographolide also showed strong oviposition deterrence effects at the sub-lethal dose of 12 ppm. Similarly, the mean number of eggs were also significantly reduced in a dose dependent manner. At the concentration of 12 ppm the effective percentage of repellency was greater than 90% with a protection time of 15-210 min, compared with control. The histopathology study displayed that larvae treated with bioactive andrographolide had cytopathic effects in the midgut epithelium compared with the control. The present study established that bioactive andrographolide served as a potential useful for dengue vector management. (C) 2016 Elsevier B.V. All rights reserved. C1 [Edwin, Edward-Sam; Vasantha-Srinivasan, Prabhakaran; Senthil-Nathan, Sengottayan; Thanigaivel, Annamalai; Ponsankar, Athirstam; Pradeepa, Venkatraman; Selin-Rani, Selvaraj] Manonmaniam Sundaranar Univ Alwarkurichi, Sri Paramakalyani Ctr Excellence Environm Sci, Div Biopesticides & Environm Toxicol, Tirunelveli 627412, Tamil Nadu, India. [Kalaivani, Kandaswamy] Sri Parasakthi Coll Women, Dept Zool, Post Grad & Res, Tirunelveli 627802, Tamil Nadu, India. [Hunter, Wayne B.] USDA, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. [Abdel-Megeed, Ahmed] Alexandria Univ, Saba Basha, Fac Agr, Dept Plant Protect, POB 21531, Alexandria 21526, Egypt. [Edwin, Edward-Sam; Duraipandiyan, Veeramuthu; Al-Dhabi, Naif Abdullah] King Saud Univ, Coll Sci, Addiriyah Chair Environm Studies, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia. RP Senthil-Nathan, S (reprint author), Manonmaniam Sundaranar Univ Alwarkurichi, Sri Paramakalyani Ctr Excellence Environm Sci, Div Biopesticides & Environm Toxicol, Tirunelveli 627412, Tamil Nadu, India. EM senthil@msuniv.ac.in RI Veeramuthu, Duraipandiyan/D-9924-2012; OI Veeramuthu, Duraipandiyan/0000-0002-2107-3503; Abdel-Megeed, Ahmed/0000-0002-9777-4808 FU King Saud University; Vice Deanship of Research Chair FX The project was full financially supported by King Saud University, through Vice Deanship of Research Chair. NR 56 TC 0 Z9 0 U1 16 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0001-706X EI 1873-6254 J9 ACTA TROP JI Acta Trop. PD NOV PY 2016 VL 163 BP 167 EP 178 DI 10.1016/j.actatropica.2016.07.00 PG 12 WC Parasitology; Tropical Medicine SC Parasitology; Tropical Medicine GA DW3FO UT WOS:000383527700025 PM 27443607 ER PT J AU Garcia-Fuentes, PA Kennedy, PL Ferreira, GFC AF Garcia-Fuentes, Pablo A. Kennedy, P. Lynn Ferreira, Gustavo F. C. TI US foreign direct investment in Latin America and the Caribbean: a case of remittances and market size SO APPLIED ECONOMICS LA English DT Article DE Foreign direct investment; remittances; market size; United States; Latin America and the Caribbean ID EXCHANGE-RATES; ECONOMETRIC-ANALYSIS; DEVELOPING-COUNTRIES; DETERMINANTS; TRADE; GROWTH; FLOWS; TIME AB This article investigates the effect of remittances on U.S. foreign direct investment (FDI) flows to Latin America and the Caribbean (LAC). It covers 26 countries for the period 1983-2010. The results show a positive and significant impact of remittances on U.S. FDI flows. However, this effect depends upon the level of gross domestic product (GDP) per capita of the host country. On average, the results show that increasing remittances by one standard deviation increases U.S. FDI flows by 0.44 percent a year. Also, host country demand positively affects U.S. FDI flows, which supports the market size hypothesis. C1 [Garcia-Fuentes, Pablo A.] Midwestern State Univ, Dillard Coll Business Adm 215, Dept Econ, Wichita Falls, TX 76308 USA. [Kennedy, P. Lynn] Louisiana State Univ, Dept 0gr Econ & Agribusiness, Baton Rouge, LA 70803 USA. [Ferreira, Gustavo F. C.] USDA, Econ Res Serv, Crops Branch, Washington, DC 20002 USA. RP Ferreira, GFC (reprint author), USDA, Econ Res Serv, Crops Branch, Washington, DC 20002 USA. EM gustavo.ferreira@ers.usda.gov NR 49 TC 0 Z9 0 U1 15 U2 15 PU ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD PI ABINGDON PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0003-6846 EI 1466-4283 J9 APPL ECON JI Appl. Econ. PD NOV PY 2016 VL 48 IS 51 BP 5008 EP 5021 DI 10.1080/00036846.2016.1170931 PG 14 WC Economics SC Business & Economics GA DW2AB UT WOS:000383444200007 ER PT J AU Itkin, D Goldfus, H Monger, HC AF Itkin, Danny Goldfus, Haim Monger, H. Curtis TI Human induced calcretisation in anthropogenic soils and sediments: Field observations and micromorphology in a Mediterranean climatic zone, Israel SO CATENA LA English DT Article DE Calcrete; Pedogenic carbonate; Calcrete cement; Geoarchaeology; Soil micromorphology ID CALCIUM-OXALATE; CALCRETE FORMATION; CARBONATE; QUATERNARY; PRECIPITATION; ECOSYSTEM; PLANTS; BIOMINERALIZATION; TRANSFORMATIONS; MINERALIZATION AB For over a century, the study of pedogenic calcrete end its formation focuses on natural processes, disregarding the possible role of humans in its formation. Here we present field and micromorphological evidence from archaeological and modern sites in the eastern Mediterranean region (Israel) that indicate that some specific calcretes are human induced. We demonstrate that anthropogenic affected calcrete differs from 'natural' (non-human induced) calcrete. We show that the occurrence of the human induced calcrete is spatially limited and is associated with both macro- and micro-scale remnants, and occasionally high content of organic material. We propose an operative definition for anthropogenic calcretisation as the interference of humans with a component(s) that influence the formation of calcrete. We assume that if present-day calcretised environments will retain their calcretisation prone conditions for the next 5-10 millennia, a distinct calcretic morphology could become a significant terrestrial formation. This discovery can be particularly useful for evaluating past human impact on the environment, adding a new archaeological set of research tools that should further be developed. (C) 2016 Elsevier B.V. All rights reserved. C1 [Itkin, Danny; Goldfus, Haim] Ben Gurion Univ Negev, Dept Bible Archaeol & Ancient Near East, Beer Sheva, Israel. [Monger, H. Curtis] USDA, Natl Soil Survey Ctr, Lincoln, NE USA. RP Itkin, D (reprint author), Ben Gurion Univ Negev, Dept Bible Archaeol & Ancient Near East, Beer Sheva, Israel. EM danitkin@gmail.com; hgoldfus@bgu.ac.il; curtis.monger@lin.usda.gov OI Itkin, Danny/0000-0002-1732-6950 NR 112 TC 0 Z9 0 U1 12 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD NOV PY 2016 VL 146 BP 48 EP 61 DI 10.1016/j.catena.2016.06.025 PG 14 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA DW8VK UT WOS:000383933400005 ER PT J AU Brevik, EC Homburg, JA Miller, BA Fenton, TE Doolittle, JA Indorante, SJ AF Brevik, Eric C. Homburg, Jeffrey A. Miller, Bradley A. Fenton, Thomas E. Doolittle, James A. Indorante, Samuel J. TI Selected highlights in American soil science history from the 1980s to the mid-2010s SO CATENA LA English DT Article DE Soil science history; Pedoarchaeology; Digital soil mapping; Soil and the environment; Geophysical instrumentation; Remote sensing ID PREHISTORIC AGRICULTURAL TERRACES; GROUND-PENETRATING RADAR; UNITED-STATES; CARBON SEQUESTRATION; ORGANIC-CARBON; ELECTRICAL-CONDUCTIVITY; NEW-MEXICO; SURVEY INFORMATION; CLIMATE-CHANGE; SUBAQUEOUS SOILS AB The beginning of the 20th century through the 1970s were good times for soil science in the USA, with relatively strong funding and overall growth in the profession. However, the soil science discipline in the USAhit hard times in the 1980s and 1990s. Federal funding for soil survey work began to decline as did student numbers in university programs and membership in the Soil Science Society of America (SSSA). Despite this, there were still many positive advances within soil science in theUSA during these two decades. There was an increased use of geophysical instrumentation, remote sensing, geographic information systems (GIS), and global positioning systems (GPS), and research began in digital soil mapping, all of which lead to better understanding of the spatial distribution and variability of soils. Many NRCS soil products were put online, making them widely available to the general public, the use of soil knowledge was expanded into new areas such as archaeology and environmental work, and historic connections to geology were re-established. While expansion into new areas required soil science to evolve as a field, separating the discipline to an extent from its agricultural roots, it also helped reinvigorate the discipline. As we move through the early parts of the 21st century, student numbers are increasing in university soil science programs and membership in SSSA is at an all-time high. Digital soil mapping is being incorporated into the National Cooperative Soil Survey, and,the impact of humans on the soil system is being fully recognized. The importance of soils is being recognized by events such as the United Nations declaration of 2015 as the "International Year of Soils". The expansion of soils into new areas and widening recognition of the importance of soils gives the field hope for a bright future in the USA. (C) 2016 Elsevier B.V. All rights reserved. C1 [Brevik, Eric C.] Dickinson State Univ, Dept Nat Sci, Dickinson, ND 58601 USA. [Homburg, Jeffrey A.] Stat Res Inc, Tucson, AZ USA. [Homburg, Jeffrey A.] Univ Arizona, Sch Anthropol, Tucson, AZ USA. [Miller, Bradley A.; Fenton, Thomas E.] Iowa State Univ, Dept Agron, Ames, IA USA. [Miller, Bradley A.] Leibniz Ctr Agr Landscape Res ZALF eV, Inst Soil Landscape Res, Eberswalder Str 84, D-15374 Muncheberg, Germany. [Doolittle, James A.] USDA NRCS NSSC, 11 Campus Blvd,Suite 200, Newtown Sq, PA USA. [Indorante, Samuel J.] USDA NRCS, Du Quoin, IL USA. RP Brevik, EC (reprint author), Dickinson State Univ, Dept Nat Sci, Dickinson, ND 58601 USA. EM Eric.Brevik@dickinsonstate.edu; jhomburg@sricrm.com; millerba@iastate.edu; tefenton@iastate.edu; sjindorante@siu.edu FU National Science Foundation [IIA-1355466] FX The authors wish to acknowledge the influence of Dan H. Yaalon in growing the study of soil science history. The early seeds for this project were planted in part during conversations between E. Brevik and D. Yaalon. We thank Pam Thomas (USDA-NRCS) for supplying Federal soil survey budget information and Jon Sandor for review of an early draft and helpful comments that improved the paper. We also thank Johan Bouma and an anonymous reviewer for their helpful comments on the manuscript. E.C. Brevik was partially supported by the National Science Foundation under Grant Number IIA-1355466 during this project. NR 284 TC 0 Z9 0 U1 38 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD NOV PY 2016 VL 146 BP 128 EP 146 DI 10.1016/j.catena.2016.06.021 PG 19 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA DW8VK UT WOS:000383933400012 ER PT J AU Brevik, EC Richter, DD Verrecchia, EP Ryan, J Poch, RM Crouvi, O Sauer, D Waroszewski, J Solleiro-Rebolledo, E Monger, C Ottner, F Targulian, V AF Brevik, Eric C. Richter, Daniel deB Verrecchia, Eric P. Ryan, John Poch, Rosa M. Crouvi, Onn Sauer, Daniela Waroszewski, Jaroslaw Solleiro-Rebolledo, Elizabeth Monger, Curtis Ottner, Franz Targulian, Victor TI The influence of Dan H. Yaalon: His impact on people SO CATENA LA English DT Article DE Pedology; Paleosols; Soil science history; Aeolian processes; Scientific contributions; Scientific influence ID REG SOILS; DESERT; LOESS; MODEL; DUST AB There are many ways to measure the accomplishments of a scientist, such as papers and books published, citations, and prestige of journals published in, but we rarely gather information that allows us to evaluate the variety of influences that individual scientists have on the careers of others. Dan H. Yaalon was one of the most accomplished pedologists of the 20th and early 21st centuries. In this paper we gather personal testimonials of the impact Yaalon had on scientists working in the areas of interest to Yaalon. This allows us to qualitatively evaluate the impact of an influential scientist on his field through the ways that he influenced the career choices of others. We found that this influence occurred through 1) personal, face-to-face contact, 2) written correspondence between Yaalon and other scientists, both through letters and through email, and 3) Yaalon's written record itself, without any direct, personal interaction between Yaalon and the other scientists. This influence took place over multiple generations of scientists, ranging from individuals who are currently retired to those who are just now in the very early stages of their scientific careers. The Yaalon example makes clear how successful scientists make significant contributions to their fields of interest well beyond those indicated by commonly used measures. (C) 2015 Elsevier B.V. All rights reserved. C1 [Brevik, Eric C.] Dickinson State Univ, Dept Nat Sci, Dickinson, ND 58601 USA. [Richter, Daniel deB] Duke Univ, Nicholas Sch Environm, Durham, NC USA. [Verrecchia, Eric P.] Univ Lausanne, Fac Geosci & Environm, Inst Earth Surface Dynam, CH-1015 Lausanne, Switzerland. [Ryan, John] Carrigataha, Cahir, Tipperary, Ireland. [Ryan, John] Amer Univ Beirut, Beirut, Lebanon. [Ryan, John] ICARDA, Aleppo, Syria. [Poch, Rosa M.] Univ Lleida, Dept Medi Ambient & Ciencies Sol, Lleida 25198, Spain. [Crouvi, Onn] Geol Survey Israel, Jerusalem, Israel. [Sauer, Daniela] Univ Gottingen, Inst Geog, Gottingen, Germany. [Waroszewski, Jaroslaw] Wroclaw Univ Environm & Life Sci, Inst Soil Sci & Environm Protect, Wroclaw, Poland. [Solleiro-Rebolledo, Elizabeth] Univ Nacl Autonoma Mexico, Mexico City, DF, Mexico. [Monger, Curtis] USDA NRCS, Soil Sci Div, Lincoln, NE USA. [Monger, Curtis] New Mexico State Univ, Las Cruces, NM 88003 USA. [Ottner, Franz] Univ Nat Resources & Life Sci, Vienna Inst Appl Geol, Vienna, Austria. [Targulian, Victor] Russian Acad Sci, Inst Geog, Moscow, Russia. RP Brevik, EC (reprint author), Dickinson State Univ, Dept Nat Sci, Dickinson, ND 58601 USA. EM Eric.Brevik@dickinsonstate.edu; drichter@duke.edu; Eric.Verrecchia@unil.ch; ryanjohn1944@gmail.com; rosa.poch@macs.udl.cat; crouvi@gsi.gov.il; Daniela.Sauer@geo.uni-goettingen.de; jaroslaw.waroszewski@gmail.com; solleiro@geologia.unam.mx; Curtis.Monger@lin.usda.gov; franz.ottner@boku.ac.at; votargulian@gmail.com RI Poch, Rosa/O-2357-2014; Waroszewski, Jaroslaw/A-1300-2017 OI Poch, Rosa/0000-0001-8639-4204; NR 36 TC 2 Z9 2 U1 11 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD NOV PY 2016 VL 146 BP 147 EP 154 DI 10.1016/j.catena.2015.10.012 PG 8 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA DW8VK UT WOS:000383933400013 ER PT J AU Acebes-Doria, AL Leskey, TC Bergh, JC AF Acebes-Doria, Angelita L. Leskey, Tracy C. Bergh, J. Christopher TI Injury to apples and peaches at harvest from feeding by Halyomorpha halys (Stal) (Hemiptera: Pentatomidae) nymphs early and late in the season SO CROP PROTECTION LA English DT Article DE Tree fruit; Brown marmorated stink bug; Malus domestica; Prunus persica ID MARMORATED STINK BUG; TREE FRUIT; HETEROPTERA; ORCHARDS; ATTRACTION; BEHAVIOR AB Halyomorpha halys (Stal) (Hemiptera: Pentatomidae) is an invasive species that has become an important orchard pest in the Mid-Atlantic region of the US. Adults and nymphs feed on tree fruit. Feeding injury from adults has been characterized but the injury from nymphs has not been examined systematically. Since the four plant-feeding instars of H. halys (second through fifth) differ substantially in size, it is plausible that the effects of their feeding on fruit injury and injury expression may differ among them. We compared feeding injury at harvest from young nymphs (second plus third instars), older nymphs (fourth plus fifth instars), and adults that were caged on 'Smoothee Golden' apples and 'Redhaven' peaches in early June (peach and apple), late July (peach), and late August (apple). Individual apples and peaches were caged at fruit set and assigned to the following treatments (n = 28/treatment): 1) control (no H. halys), 2) young nymphs or 3) adults early in the season, and 4) young nymphs, 5) older nymphs or 6) adults later in the season. Fruit in each treatment were exposed to 3-4 young nymphs, two older nymphs or 1-2 adults placed in the cages for 96 h and evaluated for external and internal feeding injury within 36 h after harvest. No injury was recorded from unexposed peaches or apples. The percentage of injured fruit and number of injuries per fruit varied significantly among the exposed treatments. Early season feeding by young nymphs yielded the least injury to peaches and apples. In apples, the highest percentage of injured fruit and number of injuries per fruit were caused by late season feeding by adults. In peaches, early season adult feeding produced the highest percentage of injured fruit and injuries per fruit. More internal than external injury was recorded on peach and no such difference was observed on apple. The implications of these findings on H. halys management in fruit orchards are discussed. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Acebes-Doria, Angelita L.; Bergh, J. Christopher] Virginia Tech, Alson H Smith Jr Agr Res & Extens Ctr, Winchester, VA 22602 USA. [Leskey, Tracy C.] USDA ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. RP Acebes-Doria, AL (reprint author), Virginia Tech AHSAREC, 595 Laurel Grove Rd, Winchester, VA 22602 USA. EM aacebes@vt.edu FU National Institute of Food and Agriculture, U.S. Department of Agriculture [2011-51181-30937] FX We sincerely grateful to Jean Engelman, Eliezer Doria and the 2015 summer interns, Sophia Dutterer, Rachel Wilk, Jason Chou and Brendan Yarbrough for their excellent technical assistance. This material is based upon work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award number #2011-51181-30937. NR 27 TC 0 Z9 0 U1 15 U2 15 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD NOV PY 2016 VL 89 BP 58 EP 65 DI 10.1016/j.cropro.2016.06.022 PG 8 WC Agronomy SC Agriculture GA DW7JG UT WOS:000383826400009 ER PT J AU Anderson, A Slootmaker, C Harper, E Holderieath, J Shwiff, SA AF Anderson, Aaron Slootmaker, Chris Harper, Erin Holderieath, Jason Shwiff, Stephanie A. TI Economic estimates of feral swine damage and control in 11 US states SO CROP PROTECTION LA English DT Article DE Feral swine; Invasive species; Crop damage; Economics; Survey ID CONSEQUENCES; RANGE AB We report the results of one of the most comprehensive surveys on feral swine (Sus scrofa) damage and control in 11 US states (Alabama, Arkansas, California, Florida, Georgia, Louisiana, Mississippi, Missouri, North Carolina, South Carolina and Texas). The survey was distributed by the USDA National Agricultural Statistical Service in the summer of 2015 to a sample of producers of corn (Zea mays), soybeans (Glycine max), wheat (Triticum), rice (Oryza sativa), peanuts (Arachis hypogaea), and sorghum (Sorghum bicolor) in the 11-state region. Producers that failed to respond to the initial mailing received multiple follow-up phone calls in an attempt to minimize non-response bias, and a total of 4377 responses were obtained. Findings indicate that damage can be substantial. The highest yield loss estimates occur in peanut and corn production in the Southeast and Texas. Control efforts are common, and producers incur considerable costs from shooting and trapping efforts. Extrapolating crop damage estimates to the state level in 10 states with reportable damage yields an estimated crop loss of $190 million. Though large, this number likely represents only a small fraction of the total damage by feral swine in the 10 states because it only includes crop damage to six crops. We hope findings from this survey will help guide control efforts and research, as well as serve as a benchmark against which the effectiveness of future control efforts can be measured. Published by Elsevier Ltd. C1 [Anderson, Aaron; Slootmaker, Chris; Harper, Erin; Holderieath, Jason; Shwiff, Stephanie A.] USDA APHIS WS, Natl Wildlife Res Ctr, 4101 Laporte Ave, Ft Collins, CO 80521 USA. RP Anderson, A (reprint author), USDA APHIS WS, Natl Wildlife Res Ctr, 4101 Laporte Ave, Ft Collins, CO 80521 USA. EM aaron.m.anderson@aphis.usda.gov NR 14 TC 0 Z9 0 U1 19 U2 19 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD NOV PY 2016 VL 89 BP 89 EP 94 DI 10.1016/j.cropro.2016.06.023 PG 6 WC Agronomy SC Agriculture GA DW7JG UT WOS:000383826400013 ER PT J AU Accinelli, C Abbas, HK Little, NS Kotowicz, JK Mencarelli, M Shier, WT AF Accinelli, Cesare Abbas, Harried K. Little, Nathan S. Kotowicz, Jeremy K. Mencarelli, Mariangela Shier, W. Thomas TI A liquid bioplastic formulation for film coating of agronomic seeds SO CROP PROTECTION LA English DT Article DE Biodegradable plastic; Seed treatment; Maize; Oilseed rape; Pesticides; Trichoderma ID ASPERGILLUS-FLAVUS; TRICHODERMA-HARZIANUM; BIOCONTROL; GERMINATION; STRAIN; CORN AB Interest in industrial and domestic applications of biodegradable plastics from renewable sources is increasing, but their use in agriculture is still limited (e.g., mulching films, plant pots, and plant clips). However, a sprayable liquid bioplastic formulation was recently evaluated for application of microbial biocontrol agents to agricultural and horticultural crops. In this study bioplastic formulation has been evaluated for use hi film-coating seeds of two agronomic species, corn and canola. Bioplastic seed coating was achieved using procedures and equipment designed for commercial polymer film-coating of tablets. Germination of both species was unaffected by the thin bioplastic coating. Bioplastic coatings containing spores of the plant-growth promoting fungus, Trichoderma harzianum, significantly stimulated the growth of corn and canola seeds. In corn seedlings, shoot and root lengths were 29% and 44% longer, respectively, in seeds coated with T harzianum-containing bioplastic than in uncoated seeds. Similarly, in canola seedlings shoot and root lengths were 19% and 20% longer, respectively, in seeds coated with T harzianum-containing bioplastic than in uncoated seeds. In a paper-roll assay, including T harzianum spores prevented reduced germination caused by a mixture of the insecticide imidacloprid and the fungicide metalaxyl-M in bioplastic seed coatings. The adhesive and plastic properties of bioplastic reduced dust-off from bioplastic-coated seeds by 96% in corn and 99% in canola compared to seeds coated with a commercial polymer. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Accinelli, Cesare; Mencarelli, Mariangela] Alma Mater Studiorum Univ Bologna, Dept Agr Sci, I-40127 Bologna, Italy. [Abbas, Harried K.; Kotowicz, Jeremy K.] USDA ARS, Biol Control Pests Res Unit, Stoneville, MS 38776 USA. [Little, Nathan S.] USDA ARS, Southern Insect Management Res Unit, Stoneville, MS 38776 USA. [Shier, W. Thomas] Univ Minnesota, Coll Pharm, Dept Med Chem, Minneapolis, MN 55455 USA. RP Accinelli, C (reprint author), Alma Mater Studiorum Univ Bologna, Dept Agr Sci, I-40127 Bologna, Italy. EM cesare.accinelli@unibo.it NR 29 TC 0 Z9 0 U1 31 U2 31 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD NOV PY 2016 VL 89 BP 123 EP 128 DI 10.1016/j.cropro.2016.07.010 PG 6 WC Agronomy SC Agriculture GA DW7JG UT WOS:000383826400018 ER PT J AU Cai, H Wang, LC Yang, ZX Wan, QL Wei, W Davis, RE Zhao, Y AF Cai, H. Wang, L. C. Yang, Z. X. Wan, Q. L. Wei, W. Davis, R. E. Zhao, Y. TI Evidence for the role of an invasive weed in widespread occurrence of phytoplasma diseases in diverse vegetable crops: Implications from lineage-specific molecular markers SO CROP PROTECTION LA English DT Article DE Phytoplasma; Subgroup 16SrII-A; Parthenium weed; Immunodominant membrane protein ID 16S RIBOSOMAL-RNA; IMMUNODOMINANT MEMBRANE-PROTEIN; ONION YELLOWS PHYTOPLASMA; CANDIDATUS-PHYTOPLASMA; WITCHES-BROOM; RFLP ANALYSIS; GENES; AURANTIFOLIA; DETERMINES; MOLLICUTES AB During the period from 2011 to 2013, several plant diseases repeatedly occurred in vegetable crops grown in Yuanmou County, Yunnan Province, China. Affected plants included cowpea, sword bean, string bean, tomato, lettuce, and water spinach. The diseased plants exhibited symptoms of witches'-broom growth and floral deformations, linking each disease to phytoplasmal infection. Phylogenetic and virtual RFLP analyses of the phytoplasmal 16S rRNA gene sequences amplified from DNA of diseased plants revealed that all of the individual strains present in the diverse vegetable plants were affiliated with a single 'Candidatus Phytoplasma' species ('Ca. Phytoplasma aurantifolia') and a single ribosomal subgroup (16SrII-A). While presence of subgroup 16SrII-A phytoplasma in this geographic region was reported previously, such widespread infections in diverse plant hosts are unveiled for the first time in this study. In pursuing the source of the infections, we found that areas surrounding the affected vegetable fields were extensively invaded by parthenium weeds (Parthenium hysterophorus); and many of the weed plants exhibited abnormal morphologies that were suspicious of, and later diagnosed with, phytoplasmal infections. Results from genotyping of 16S rRNA and lineage-specific immunodominant membrane protein genes revealed that the vegetable-infecting phytoplasmas and the parthenium weed phytoplasma belong to the same genetic lineage. The findings indicate that parthenium weed poses a substantial risk as a reservoir of phytoplasmal infection of nearby agricultural crops in the geographic region since the ecosystems of Yuanmou are insect-rich, and parthenium weed is known to attract diverse leafhoppers. Further studies are warranted to assess the impact of farmland invasions by the noxious weed and to devise practical measures for improved weed control. Published by Elsevier Ltd. C1 [Cai, H.; Wan, Q. L.] Yunnan Agr Univ, Key Lab Plant Pathol, Kunming 650201, Peoples R China. [Wang, L. C.; Wan, Q. L.] Yuxi Normal Univ, Coll Resources & Environm, Yuxi 653100, Peoples R China. [Yang, Z. X.] Yunnan Acad Agr Sci, Inst Trop Ecoagr Sci, Yuanmou 651300, Peoples R China. [Wei, W.; Davis, R. E.; Zhao, Y.] ARS, Mol Plant Pathol Lab, USDA, Beltsville, MD 20705 USA. RP Zhao, Y (reprint author), ARS, Mol Plant Pathol Lab, USDA, Beltsville, MD 20705 USA. EM yan.zhao@ars.usda.gov FU Program for Innovative Research Team (in Science and Technology) in University of Yunnan Province, IRTSTYN [2014-2022]; National Natural Science Foundation of China [31060239]; U.S. Department of Agriculture, Agricultural Research Service [1245-22000-282-00D] FX This research was supported by the Program for Innovative Research Team (in Science and Technology) in University of Yunnan Province, IRTSTYN (2014-2022), the National Natural Science Foundation of China (Grant number 31060239) and the U.S. Department of Agriculture, Agricultural Research Service (Project number1245-22000-282-00D). NR 43 TC 0 Z9 0 U1 25 U2 25 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD NOV PY 2016 VL 89 BP 193 EP 201 DI 10.1016/j.cropro.2016.07.025 PG 9 WC Agronomy SC Agriculture GA DW7JG UT WOS:000383826400026 ER PT J AU Maffei, HM Filip, GM Grulke, NE Oblinger, BW Margolis, EQ Chadwick, KL AF Maffei, Helen M. Filip, Gregory M. Grulke, Nancy E. Oblinger, Brent W. Margolis, Ellis Q. Chadwick, Kristen L. TI Pruning high-value Douglas-fir can reduce dwarf mistletoe severity and increase longevity in Central Oregon SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Pseudotsuga menziesii var. menziesii; Arceuthobium douglasii; Pruning; Tree rings; Normalized Difference Vegetation Index (NDVI); Oregon ID WESTERN SPRUCE BUDWORM; GROWTH; ARCEUTHOBIUM; MORTALITY; LEAF AB Mid- to very large-sized Douglas-fir (Pseudotsuga menzieseii var. menziesii) that were lightly- to moderately-infected by dwarf mistletoe (Arceuthobium douglasii) were analyzed over a 14-year period to evaluate whether mechanical pruning could eradicate mistletoe (or at least delay the onset of severe infection) without significantly affecting tree vitality and by inference, longevity. Immediate and long-term pruning effects on mistletoe infection severity were assessed by comparing pruned trees (n = 173) to unpruned trees (n = 55) with respect to: (1) percentage of trees with no visible infections 14 years post-pruning, (2) Broom Volume Rating (BVR), and (3) rate of BVR increase 14 years post pruning. Vitality/longevity (compared with unpruned trees) was assessed using six indicators: (1) tree survival, (2) the development of severe infections, (3) the development of dead tops, (4) tree-ring width indices, (5) Normalized Difference Vegetation Index (NDVI) from high-resolution multi-spectral imagery, and (6) live-crown ratio (LCR) and increment. Twenty-four percent of the pruned trees remained free of mistletoe 14 years post-pruning. Pruning is most likely to successfully eradicate mistletoe in lightly infected trees (BVR 1 or 2) without infected neighbors. Pruning significantly decreased mean BVR in the pruned versus the unpruned trees. However, the subsequent average rate of intensification (1.3-1.5 BVR per decade) was not affected, implying that a single pruning provides 14 years respite in the progression of infection levels. Post-pruning infection intensification was slower on dominant and co-dominants than on intermediate or suppressed trees. The success of mistletoe eradication via pruning and need for follow-up pruning should be evaluated no sooner than 14 years after pruning to allow for the development of detectable brooms. Based on six indicators, foliage from witches brooms contribute little to long-term tree vitality since removal appears to have little effect on resources available for tree growth and maintenance. In the severely pruned trees, tree-ring width was reduced for several years post-pruning, but then compensated with larger ring width in later years. Both NDVI and LCR increment were significantly higher for the pruned trees than the control trees, while the development of severe infections and/or dead tops was significantly (5x and 3 x) higher for the controls. If possible, multiple indicators of tree vitality should be evaluated. Pruning can be worthwhile even if all the mistletoe is not removed, because mistletoe intensification is delayed. The impact of removing the brooms seems to be minimal, and post-pruning crowns had greater NDVI values. Published by Elsevier B.V. C1 [Maffei, Helen M.; Oblinger, Brent W.] US Forest Serv, USDA, 63095 Deschutes Market Rd, Bend, OR 97701 USA. [Filip, Gregory M.] US Forest Serv, USDA, Pacific Northwest Reg Off, 1220 SW Third Ave, Portland, OR 97204 USA. [Grulke, Nancy E.] US Forest Serv, USDA, Western Wildland Environm Threat Assessment Ctr, 3160 NE 3rd St, Prineville, OR 97754 USA. [Margolis, Ellis Q.] US Geol Survey, Ft Collins Sci Ctr, Jemez Mt Field Stn, 301 Dinosaur Trail, Santa Fe, NM 87508 USA. [Chadwick, Kristen L.] US Forest Serv, USDA, 16400 Champion Way, Sandy, OR 97055 USA. RP Maffei, HM (reprint author), US Forest Serv, USDA, 63095 Deschutes Market Rd, Bend, OR 97701 USA. EM hmaffei@fs.fed.us NR 35 TC 0 Z9 0 U1 10 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD NOV 1 PY 2016 VL 379 BP 11 EP 19 DI 10.1016/j.foreco.2016.07.014 PG 9 WC Forestry SC Forestry GA DW7FM UT WOS:000383816600002 ER PT J AU Bradley, JC Will, RE Stewart, JF Nelson, CD Guldin, JM AF Bradley, J. C. Will, R. E. Stewart, J. F. Nelson, C. D. Guldin, J. M. TI Post-fire resprouting of shortleaf pine is facilitated by a morphological trait but fire eliminates shortleaf x loblolly pine hybrid seedlings SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Basal crook; Fire; Pinus echinata; Pinus taeda; Resprouting; Shortleaf x loblolly pine hybrid ID SHAPING PLANT TRAITS; CLIMATE AB Concurrent with fire exclusion, shortleaf pine x loblolly pine hybrids have increased throughout the southeastern USA and may threaten the genetic integrity of shortleaf pine. Historically, fire favored short leaf pine over other southern pine species, especially loblolly pine, with which it shares a broad sympatric natural range. Shortleaf pine seedlings have a morphological adaptation (basal crook) that lowers dormant buds to the soil surface where they are presumably protected from fire to facilitate resprouting after topkill. To evaluate this presumption, we tested (1) the functional role of the basal crook in protecting dormant buds by measuring resprouting after exposing the lower stem of shortleaf pine to fire and protecting from fire the lower stem (and dormant buds) of loblolly pine (which lacks a basal crook) and (2) the occurrence and importance of the basal crook morphological adaptation in Fl shortleaf x loblolly pine seedlings by comparing morphology and post-fire resprouting of the hybrids to both parents. Fire exposure of shortleaf pine seedling dormant buds caused seedling mortality, while protecting loblolly pine dormant buds facilitated resprouting. Hybrid pines have basal crooks intermediate to the strong crook of shortleaf pine and loblolly pine's non-crooked stem. Fire top-killed loblolly pine and short leaf x loblolly pine seedlings, and they did not resprout, while 57% of shortleaf pine resprouted after top kill during two dormant season and one growing season burns. This highly significant difference shows that the basal crook is important for shortleaf pine resprouting after topkill by fire. Fire is an important tool to reduce shortleaf x loblolly pine hybrids, and to provide a competitive advantage to shortleaf pine relative to loblolly pine, for ecosystem restoration and maintaining the genetic integrity of shortleaf pine. (C) 2016 Elsevier B.V. All rights reserved. C1 [Bradley, J. C.; Will, R. E.] Oklahoma State Univ, Dept Nat Resources Ecol & Management, 008C Agr Hall, Stillwater, OK 74078 USA. [Stewart, J. F.] Oklahoma State Univ, Dept Integrat Biol, Stillwater, OK 74078 USA. [Nelson, C. D.] US Forest Serv, USDA, Southern Res Stn, Southern Inst Forest Genet, 23332 Success Rd, Saucier, MS 39574 USA. [Nelson, C. D.] Univ Kentucky, Forest Hlth Res & Educ Ctr, 208 TP Cooper Hall, Lexington, KY 40546 USA. [Guldin, J. M.] US Forest Serv, USDA, Southern Res Stn, 607 Reserve St, Hot Springs, AR 71902 USA. RP Will, RE (reprint author), Oklahoma State Univ, Dept Nat Resources Ecol & Management, 008C Agr Hall, Stillwater, OK 74078 USA. EM rodney.will@okstate.edu RI Will, Rodney/G-8111-2011 FU Oklahoma Agricultural Experimental Station; McIntire-Stennis project [OKL0 2929]; USDA Forest Service Cooperative Agreement [14-CA-11330126-104] FX We would like to thank the Oklahoma Agricultural Experimental Station, McIntire-Stennis project OKL0 2929, and USDA Forest Service Cooperative Agreement 14-CA-11330126-104 for providing funding for this research. We are deeply grateful for the assistance from Bob Heinemann and the staff at the Kiamichi Forestry Research Center, including Cassandra Meek, Randy Holeman, Dennis Wilson, Glenda Robinson, and Keith Anderson. We would also like to thank Professor Emeritus Charles Tauer for making the shortleaf x loblolly crosses and initiating this line of research. We also appreciated the help of Duncan Wilson, Adam Maggard, Dipesh KC, Lays Francisco, Rodrigo Carvalhais, and Casey Ausmus at various crucial points NR 26 TC 0 Z9 0 U1 7 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD NOV 1 PY 2016 VL 379 BP 146 EP 152 DI 10.1016/j.foreco.2016.08.016 PG 7 WC Forestry SC Forestry GA DW7FM UT WOS:000383816600015 ER PT J AU Moser, WK Fan, ZF Hansen, MH Crosby, MK Fan, SX AF Moser, W. Keith Fan, Zhaofei Hansen, Mark H. Crosby, Michael K. Fan, Shirley X. TI Invasibility of three major non-native invasive shrubs and associated factors in Upper Midwest US forest lands SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Invasion potential; Exotic species; FIA ID PLANT INVASIONS; UNITED-STATES; PATTERNS; CONSERVATION; MANAGEMENT AB We used non-native invasive plant data from the US Forest Service's Forest Inventory and Analysis (FIA) program, spatial statistical methods, and the space (cover class)-for-time approach to quantify the invasion potential and success ("invasibility") of three major invasive shrubs (multiflora rose, non-native bush honeysuckles, and common buckthorn) in broadly classified forest-type gtoups in seven Upper Midwest states. Smoothed maps of presence and cover percent showed a strong clustering pattern for all three invasive shrubs despite their different ranges. The species are clustered around major cities or urban areas (e.g., Chicago, Illinois, and Des Moines, Iowa), indicating the potential role humans played in their invasion and spread on the landscape and throughout the Midwest. Conditional inference tree (ctree) models further quantified the significant factors contributing to the observed regional patterns: for distribution of multiflora rose, percentage of forest cover in the county (measuring human disturbance intensity) and stand density index; for distribution of common buckthorn, distance to major highways. Non-native bush honeysuckles were not associated with any disturbance and site/stand variables except for latitude and longitude. The infested FIA plots by cover class were positively associated in space, signifying a concentric-like spread trend from previously infested sites (hot spots) to surrounding areas. By forest-type groups or as a whole, the three species spread slowly at earlier stages, but recently have increased significantly in presence/expansion. Oak/hickory and elm/ash/cottonwood forests were more susceptible to the three invasive shrubs compared to other forests. We recommend that resource managers and planners prescribe control and mitigation treatments for non-native invasive plants by forest types and spatial locations close to highways and residences. Published by Elsevier B.V. C1 [Moser, W. Keith] US Forest Serv, USDA, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. [Fan, Zhaofei; Fan, Shirley X.] Auburn Univ, Auburn, AL 36849 USA. [Crosby, Michael K.] Shorter Univ, Rome, GA USA. [Hansen, Mark H.] US Forest Serv, USDA, Northern Res Stn, St Paul, MN USA. RP Moser, WK (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. EM wkmoser@fs.fed.us FU US Forest Service, Forest Health Protection, Environmental Monitoring program; US Forest Service; Northern Research Station; Forest Inventory and Analysis program; Mississippi State University; Department of Forestry FX The authors thank the US Forest Service, Forest Health Protection, Environmental Monitoring program; the US Forest Service, Northern Research Station, Forest Inventory and Analysis program; and the Mississippi State University, Department of Forestry for their support of this study. Dr. Mark D. Nelson, US Forest Service, Northern Research Station, Forest Inventory and Analysis program, provided valuable assistance with spatially joining FIA plots and other features. Benjamin Bird, Cynthia Moser and two anonymous reviewers of earlier versions of this manuscript offered valuable comments. NR 40 TC 0 Z9 0 U1 67 U2 67 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD NOV 1 PY 2016 VL 379 BP 195 EP 205 DI 10.1016/j.foreco.2016.07.043 PG 11 WC Forestry SC Forestry GA DW7FM UT WOS:000383816600020 ER PT J AU Bell, DM Gray, AN AF Bell, David M. Gray, Andrew N. TI Assessing intra- and inter-regional climate effects on Douglas-fir biomass dynamics in Oregon and Washington, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Climate; Douglas-fir; Forest inventory; Forest structure; Forest succession; Pacific Northwest; Pseudotsuga menziesii ID NEAREST-NEIGHBOR IMPUTATION; PACIFIC-NORTHWEST; UNITED-STATES; FOREST; COMMUNITIES; SUCCESSION; MODELS; GROWTH; DISTURBANCES; ECOSYSTEMS AB While ecological succession shapes contemporary forest structure and dynamics, other factors like forest structure (dense vs. sparse canopies) and climate may alter structural trajectories. To assess potential sources of variation in structural trajectories, we examined proportional biomass change for a regionally dominant tree species, Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco), across vegetation zones representing broad gradients in precipitation and temperature with 3510 forest inventory plots in Oregon and Washington, USA. We found that P. menziesii biomass change decreased with P. menziesii biomass stocks and increased with P. menziesii density, remaining positive in older stands only in the wet and warm vegetation zone. Within two of the vegetation zones, biomass change was greatest in warm and wet environments. In dry vegetation zones, positive P. menziesii biomass change responses to initial canopy cover and canopy cover change (i.e., increases with cover loss and decreases with cover gain) indicated shifts in forest structure. Variation in P. menziesii biomass dynamics within and between vegetation zones imply multi-scale climatic controls on forest structural trajectories for P. menziesii and highlight the potential for continued atmospheric carbon sequestration in warm and wet forests of the Pacific Northwest for both young and old forests, given that future climatic conditions support similar forest dynamics. Published by Elsevier B.V. C1 [Bell, David M.; Gray, Andrew N.] US Forest Serv, USDA, Pacific Northwest Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. RP Bell, DM (reprint author), 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM dmbell@fs.fed.us; agray01@fs.fed.us FU USDA Forest Service Pacific Northwest Research Station FX This research was funded by the USDA Forest Service Pacific Northwest Research Station. We thank Matthew Gregory for extracting canopy cover estimates for each plot from the GNN data. We thank Matthew Reilly, Dan Binkley, and two anonymous reviewers for providing comments on the manuscript. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the United States government. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the USDA Forest Service. NR 46 TC 0 Z9 0 U1 15 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD NOV 1 PY 2016 VL 379 BP 281 EP 287 DI 10.1016/j.foreco.2016.07.023 PG 7 WC Forestry SC Forestry GA DW7FM UT WOS:000383816600029 ER PT J AU Yen, H White, MJ Arnold, JG Keitzer, SC Johnson, MVV Atwood, JD Daggupati, P Herbert, ME Sowa, SP Ludsin, SA Robertson, DM Srinivasan, R Rewa, CA AF Yen, Haw White, Michael J. Arnold, Jeffrey G. Keitzer, S. Conor Johnson, Mari-Vaughn V. Atwood, Jay D. Daggupati, Prasad Herbert, Matthew E. Sowa, Scott P. Ludsin, Stuart A. Robertson, Dale M. Srinivasan, Raghavan Rewa, Charles A. TI Western Lake Erie Basin: Soft-data-constrained, NHDPIus resolution watershed modeling and exploration of applicable conservation scenarios SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Lake Erie; NHDPIus model; Soft data; Conservation practice; Swat-SAS; Model calibration ID DEPOSITED FINE SEDIMENT; FRESH-WATER; SWAT MODEL; INPUT DATA; MANAGEMENT-PRACTICES; DISSOLVED NUTRIENTS; MULTIPLE STRESSORS; OUTPUT UNCERTAINTY; MISSISSIPPI RIVER; ASSESSMENT-TOOL AB Complex watershed simulation models are powerful tools that can help scientists and policy-makers address challenging topics, such as land use management and water security. In the Western Lake Erie Basin (WLEB), complex hydrological models have been applied at various scales W help describe relationships between land use and water, nutrient, and sediment dynamics. This manuscript evaluated the capacity of the current Soil and Water Assessment Tool (SWAT) to predict hydrological and water quality processes within WLEB at the finest resolution watershed boundary unit (NHDPIus) along with the current conditions and conservation scenarios. The process based SWAT model was capable of the fine-scale computation and complex routing used in this project, as indicated by measured data at five gaging stations. The level of detail required for fine-scale spatial simulation made the use of both hard and soft data necessary in model calibration, alongside other model adaptations. Limitations to the model's predictive capacity were due to a paucity of data in the region at the NHDPIus scale rather than due to SWAT functionality. Results of treatment scenarios demonstrate variable effects of structural practices and nutrient management on sediment and nutrient loss dynamics. Targeting treatment to acres with critical outstanding conservation needs provides the largest return on investment in terms of nutrient loss reduction per dollar spent, relative to treating acres with lower inherent nutrient loss vulnerabilities. Importantly, this research raises considerations about use of models to guide land management decisions at very fine spatial scales. Decision makers using these results should be aware of data limitations that hinder fine-scale model interpretation. (C) 2016 Elsevier B.V. All rights reserved. C1 [Yen, Haw] Texas A&M Agrilife Res, Blackland Res & Extens Ctr, 720 East Blackland Rd, Temple, TX 76502 USA. [White, Michael J.; Arnold, Jeffrey G.] USDA ARS, Grassland Soil & Water Res Lab, 808 East Blackland Rd, Temple, TX 76502 USA. [Keitzer, S. Conor; Ludsin, Stuart A.] Ohio State Univ, Dept Evolut Ecol & Organismal Biol, Aquat Ecol Lab, 1314 Kinnear Rd, Columbus, OH 43212 USA. [Johnson, Mari-Vaughn V.; Atwood, Jay D.] USDA, Grassland Soil & Water Res Lab, Resources Assessment Div, NRCS, 808 East Blackland Rd, Temple, TX 76502 USA. [Daggupati, Prasad; Srinivasan, Raghavan] Texas A&M Univ, Spatial Sci Lab, Suite 221,1500 Res Plaza, College Stn, TX 77843 USA. [Herbert, Matthew E.; Sowa, Scott P.] Michigan Field Off, Nat Conservancy, 101 East Grand River Ave, Lansing, MI 48906 USA. [Robertson, Dale M.] US Geol Survey, Wisconsin Water Sci Ctr, 8505 Res Way, Middleton, WI 53562 USA. [Rewa, Charles A.] USDA, Resources Assessment Div, NRCS, George Washington Carver Ctr, Beltsville, MD 20705 USA. RP Yen, H (reprint author), Texas A&M Agrilife Res, Blackland Res & Extens Ctr, 720 East Blackland Rd, Temple, TX 76502 USA. EM haw.yen@gmail.com RI Srinivasan, R/D-3937-2009; Daggupati, Prasad/D-8886-2017 OI Daggupati, Prasad/0000-0002-7044-3435 FU United States Department of Agriculture - Natural Resources Conservation Service (USDA-NRCS) Conservation Effects Assessment Project (CEAP) - Wildlife and Cropland [68-7482-12-504] FX This project was funded by grants from the United States Department of Agriculture - Natural Resources Conservation Service (USDA-NRCS) Conservation Effects Assessment Project (CEAP) - Wildlife and Cropland components (Cooperative Agreement: 68-7482-12-504). USDA is an equal opportunity employer and provider! NR 93 TC 4 Z9 4 U1 24 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD NOV 1 PY 2016 VL 569 BP 1265 EP 1281 DI 10.1016/j.scitotenv.2016.06.202 PG 17 WC Environmental Sciences SC Environmental Sciences & Ecology GA DU5RM UT WOS:000382269000122 PM 27387796 ER PT J AU Lichtenberg, E Page, ET AF Lichtenberg, Erik Page, Elina Tselepidakis TI Prevalence and cost of on-farm produce safety measures in the Mid-Atlantic SO FOOD CONTROL LA English DT Article DE Food safety; Produce safety; Food safety modernization act; Tomatoes; Leafy greens; Compliance cost; Regulatory burden; Mid-Atlantic; Vegetable growers ID GOOD AGRICULTURAL PRACTICES; FOOD; SCALE AB We use data from a survey of leafy green and tomato growers in the Mid-Atlantic region to investigate the prevalence and cost of produce safety practices required under the Produce Rule of the Food Safety Modernization Act. Majorities of our respondents currently employ most of the food safety practices that will be required under the Produce Rule. But the Produce Rule will nevertheless require changes on the part of some growers. We find that farm size has a statistically and quantitatively negligible relationship with the use of most produce safety practices except for the sampling and testing of water, soil amendments, and product samples. Contrary to the theoretical literature suggesting that traceability increases incentives to take precautionary measures, we find little evidence that the use of produce safety practices is correlated with any marketing channel. We do find that all of these practices exhibit substantial increasing returns to scale, implying that the burden of complying with the provisions of the Produce Rule is much lower for large operations than small ones. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Lichtenberg, Erik] Univ Maryland, Dept Agr & Resource Econ, College Pk, MD 20742 USA. [Page, Elina Tselepidakis] USDA, Econ Res Serv, Washington, DC 20250 USA. RP Lichtenberg, E (reprint author), Univ Maryland, Dept Agr & Resource Econ, College Pk, MD 20742 USA. EM elichten@umd.edu OI Lichtenberg, Erik/0000-0003-0619-6254 FU United States Department of Agriculture/National Institute of Food and Agriculture Specialty Crop Research Initiative Grant [2011-5118130767] FX This research was supported by United States Department of Agriculture/National Institute of Food and Agriculture Specialty Crop Research Initiative Grant 2011-5118130767 to the University of Maryland, College Park. The views expressed are the authors' and may not be attributed to the Economic Research Service or the U.S. Department of Agriculture. NR 16 TC 0 Z9 0 U1 4 U2 9 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD NOV PY 2016 VL 69 BP 315 EP 323 DI 10.1016/j.foodcont.2016.04.054 PG 9 WC Food Science & Technology SC Food Science & Technology GA DQ5XM UT WOS:000379278600042 ER PT J AU Cabanillas, B Crespo, JF Maleki, SJ Rodriguez, J Novak, N AF Cabanillas, Beatriz Crespo, Jesus F. Maleki, Soheila J. Rodriguez, Julia Novak, Natalija TI Pin p 1 is a major allergen in pine nut and the first food allergen described in the plant group of gymnosperms SO FOOD CHEMISTRY LA English DT Article DE Albumin; Basophil activation test; Pin p 1; Pine nut allergy; Tree nut allergy ID PROTEINS; PEPTIDES; PEANUT AB This study aimed to report the complete sequence of a 2S albumin purified from pine nut and to analyze its allergenic properties. Individual recognition of this protein by serum IgE from pine nut-allergic patients was assessed. IgE cross-linking capacity was analyzed in a basophil activation test. Inhibition of IgE-binding and stability to heating was also assessed. The complete nucleotide sequence was obtained and a phylogenetic study was carried out. 2S albumin from pine nut (registered as Pin p 1.0101) was recognized by IgE of 75% of sera. The allergen was heat-stable and had a robust capacity to inhibit IgEbinding to whole pine nut extract. The IgE cross-linking capacity of Pin p 1 on basophils was also demonstrated. Despite the low homology of Pin p 1 sequence with other allergenic 2S albumins from angiosperms, Pin p 1 contains the typical skeleton of 8 cysteine residues, important for its a-helixes enriched structure. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Cabanillas, Beatriz; Novak, Natalija] Univ Bonn, Med Ctr, Dept Dermatol & Allergy, Sigmund Freud Str 25, D-53127 Bonn, Germany. [Crespo, Jesus F.; Rodriguez, Julia] Hosp Univ 12 Octubre, Serv Alergia, Inst Invest Hosp 12 Octubre I 12, Ave Cordoba S-N, Madrid 28041, Spain. [Maleki, Soheila J.] ARS, USDA, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA USA. RP Cabanillas, B (reprint author), Univ Bonn, Med Ctr, Dept Dermatol & Allergy, Sigmund Freud Str 25, D-53127 Bonn, Germany. EM Beatriz.Cabanillas@ukb.uni-bonn.de FU Cluster of Excellence ImmunoSensation of the German Research Foundation (DFG) (Germany); Christine Kuhne Stiftung CK-CARE; BONFOR Grant of the University of Bonn (Germany) FX The study was supported by Cluster of Excellence ImmunoSensation of the German Research Foundation (DFG) (Germany), the Christine Kuhne Stiftung CK-CARE and a BONFOR Grant of the University of Bonn (Germany). NR 20 TC 0 Z9 0 U1 18 U2 29 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD NOV 1 PY 2016 VL 210 BP 70 EP 77 DI 10.1016/j.foodchem.2016.04.068 PG 8 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA DM2FT UT WOS:000376163100010 PM 27211622 ER PT J AU Qin, YL Qiu, XQ Zhu, JY AF Qin, Yanlin Qiu, Xueqing Zhu, J. Y. TI Understanding Longitudinal Wood Fiber Ultra-structure for Producing Cellulose Nanofibrils Using Disk Milling with Diluted Acid Prehydrolysis SO SCIENTIFIC REPORTS LA English DT Article ID ENZYMATIC SACCHARIFICATION; MICROFIBRILLATED CELLULOSE; ENDOGLUCANASE TREATMENT; TRANSPARENT FILMS; LODGEPOLE PINE; HYDROLYSIS; PULP; MICROFLUIDIZATION; HOMOGENIZATION; LIGNOCELLULOSE AB Here we used dilute oxalic acid to pretreat a kraft bleached Eucalyptus pulp (BEP) fibers to facilitate mechanical fibrillation in producing cellulose nanofibrils using disk milling with substantial mechanical energy savings. We successfully applied a reaction kinetics based combined hydrolysis factor (CHFX) as a severity factor to quantitatively control xylan dissolution and BEP fibril deploymerization. More importantly, we were able to accurately predict the degree of polymerization (DP) of disk-milled fibrils using CHFX and milling time or milling energy consumption. Experimentally determined ratio of fibril DP and number mean fibril height (diameter d), DP/d, an aspect ratio measurer, were independent of the processing conditions. Therefore, we hypothesize that cellulose have a longitudinal hierarchical structure as in the lateral direction. Acid hydrolysis and milling did not substantially cut the "natural" chain length of cellulose fibrils. This cellulose longitudinal hierarchical model provides support for using weak acid hydrolysis in the production of cellulose nanofibrils with substantially reduced energy input without negatively affecting fibril mechanical strength. C1 [Qin, Yanlin] Guangdong Univ Technol, Sch Chem Engn & Light Ind, Guangzhou, Guangdong, Peoples R China. [Qiu, Xueqing] South China Univ Technol, Sch Chem & Chem Engn, Guangzhou, Guangdong, Peoples R China. [Qin, Yanlin; Zhu, J. Y.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Zhu, JY (reprint author), US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. EM jzhu@fs.fed.us FU USDA Agriculture and Food Research Initiative (AFRI) Competitive Grant [2011-67009-20056]; Chinese Scholarship Council (CSC); International Science and Technology Cooperation Program of China (ISTCP) [2013DFA41670]; National Natural Science Foundation of China [21576106] FX Financial support for this work included USDA Agriculture and Food Research Initiative (AFRI) Competitive Grant (No. 2011-67009-20056), Chinese Scholarship Council (CSC), and the International Science and Technology Cooperation Program of China (ISTCP): 2013DFA41670, National Natural Science Foundation of China (21576106). We also acknowledge Ms. Lixuan Yu, a graduate student at South China University for her help on some AFM imaging work. NR 45 TC 2 Z9 2 U1 7 U2 7 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD OCT 31 PY 2016 VL 6 AR 35602 DI 10.1038/srep35602 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EA3TD UT WOS:000386526900001 PM 27796325 ER PT J AU Wang, J Peng, YD He, C Wei, BY Liang, YS Yang, HL Wang, Z Stanley, D Song, QS AF Wang, Juan Peng, Yuan-De He, Chao Wei, Bao-Yang Liang, Yun-Shan Yang, Hui-Lin Wang, Zhi Stanley, David Song, Qi-Sheng TI Cry1Ab-expressing rice did not influence expression of fecundity-related genes in the wolf spider Pardosa pseudoannulata SO GENE LA English DT Article DE Cry1Ab protein; Vitellin; Transcriptome; P. pseudoannulata ID NILAPARVATA-LUGENS STAL; INDICA BASMATI RICE; TRANSGENIC BT RICE; CHRYSOPERLA-SINICA; BROWN PLANTHOPPER; FIELD-EVALUATION; RNA-SEQ; PROTEIN; TOOL; RESISTANCE AB The impact of Bacillus thuringiensis (Bt) toxin proteins on non-target predatory arthropods is not well understood at the cellular and molecular levels. Here, we investigated the potential effects of Cry1Ab expressing rice on fecundity of the wolf spider, Pardosa pseudoannulata, and some of the underlying molecular mechanisms. The results indicated that brown planthoppers (BPHs) reared on Cry1Ab-expressing rice accumulated the Cry toxin and that reproductive parameters (pre-oviposition period, post-oviposition stage, number of eggs, and egg hatching rate) of the spiders that consumed BPHs reared on Bt rice were not different from those that consumed BPHs reared on the non-Bt control rice. The accumulated Cry1Ab did not influence several vitellin (Vt) parameters, including stored energy and amino acid composition, during one generation. We considered the possibility that the Cry toxins exert their influence on beneficial predators via more subtle effects detectable at the molecular level in terms of gene expression. This led us to transcriptome analysis to detect differentially expressed genes in the ovaries of spiders exposed to dietary Cry1Ab and their counterpart control spiders. Eight genes, associated with vitellogenesis, vitellogenin receptor activity, and vitellin membrane formation were not differentially expressed between ovaries from the treated and control spiders, confirmed by qPCR analysis. We infer that dietary Cry1Ab expressing rice does not influence fecundity, nor expression levels of Vt-associated genes in P. pseudoannulata. (C) 2016 Published by Elsevier B.V. C1 [Wang, Juan; Wei, Bao-Yang; Liang, Yun-Shan; Yang, Hui-Lin; Wang, Zhi] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China. [Peng, Yuan-De] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China. [He, Chao] Cent S Univ, Longping Coll, Changsha 410001, Hunan, Peoples R China. [Stanley, David] ARS, USDA, Biol Control Insects Res Lab, Columbia, MO 65203 USA. [Song, Qi-Sheng] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. RP Wang, Z (reprint author), Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China. EM wangzhisipder@hotmail.com; SongQ@missouri.edu FU National Natural Science Foundation of China [31071943, 31272339]; Science Research Fund of the Education Department of Hunan Province [10 A054]; Agricultural Science and Technology Innovation Program of China [CAAS-ASTIP-2016-IBFC] FX This work was supported by the National Natural Science Foundation of China (No. 31071943, 31272339), the Science Research Fund of the Education Department of Hunan Province (No. 10 A054), and the Agricultural Science and Technology Innovation Program of China (No. CAAS-ASTIP-2016-IBFC). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. All programs and services of the U.S. Department of Agriculture are offered on a nondiscriminatory basis without regard to race, color, national origin, religion, sex, age, marital status, or handicap. NR 43 TC 0 Z9 0 U1 22 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 EI 1879-0038 J9 GENE JI Gene PD OCT 30 PY 2016 VL 592 IS 1 BP 1 EP 7 DI 10.1016/j.gene.2016.07.041 PG 7 WC Genetics & Heredity SC Genetics & Heredity GA DW0AZ UT WOS:000383304600001 PM 27452121 ER PT J AU Oblath, EA Isbell, TA Berhow, MA Allen, B Archer, D Brown, J Gesch, RW Hatfield, JL Jabro, JD Kiniry, JR Long, DS AF Oblath, Emily A. Isbell, Terry A. Berhow, Mark A. Allen, Brett Archer, David Brown, Jack Gesch, Russell W. Hatfield, Jerry L. Jabro, Jalal D. Kiniry, James R. Long, Daniel S. TI Development of near-infrared spectroscopy calibrations to measure quality characteristics in intact Brassicaceae germplasm SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Brassicaceae; Near-infrared spectroscopy; Intact seed; Oil; Total glucosinolates; Chlorophyll ID FATTY-ACID-COMPOSITION; REFLECTANCE SPECTROSCOPY; OIL CONTENT; RAPESEED QUALITY; SINGLE SEEDS; NAPUS L.; GLUCOSINOLATE; PROTEIN; CROPS; COMPONENTS AB Determining seed quality parameters is an integral part of cultivar improvement and germplasm screening. However, quality tests are often time consuming, seed destructive, and can require large seed samples. This study describes the development of near-infrared spectroscopy (NIRS) calibrations to measure moisture, oil, fatty acid profile, nitrogen, glucosinolate, and chlorophyll content in six species from the Brassicaceae family. Rapeseed and similar oilseeds are potential feedstocks for producing hydrotreated renewable jet fuel. Screening samples with NIRS would allow cultivars with desirable characteristics to be quickly identified. A total of 367 samples of six species (Brassica napus, Brassica carinata, Brassica juncea, Brassica rapa, Sinapis alba, and Camelina saliva) were scanned with NIRS. Global calibrations for all six species were developed using modified partial least squares regression with reference values obtained through wet chemistry techniques. Comparing predicted values to reference data, the coefficients of determination (r(2)) and ratios of performance to deviation (RPD) varied, with some calibrations performing better than others. The calibration equations for seed oil content (r(2) = 0.98, RPD = 7.3) and nitrogen (r(2) = 0.98, RPD = 5.3) performed very well while the equations for seed moisture (r(2) = 0.93, RPD = 3.8) and total glucosinolate content (r(2) = 0.92, RPD = 2.3) were more qualitative. Large variation was observed for chlorophyll content (0-390 mg/kg) so two calibration equations were developed, one for the higher and one for the lower range of values. When combined, these calibrations also showed very good performance (r(2) = 0.99, RPD = 14). The performance of the calibrations for the fatty acids was more varied, with some performing very well, such as the calibration for C-18:3 (r(2) = 0.99, RPD = 9.9), and others, such as C-22:0 (r(2) = 0.69, RPD = 1.9), showing poor correlation. (C) 2016 Published by Elsevier B.V. C1 [Oblath, Emily A.; Isbell, Terry A.; Berhow, Mark A.] USDA ARS, NCAUR, 1815 N Univ St, Peoria, IL 61604 USA. [Allen, Brett; Jabro, Jalal D.] USDA ARS, NPARL, Sidney, MT 59270 USA. [Archer, David] USDA ARS, NGPRL, Mandan, ND 58554 USA. [Brown, Jack] Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83844 USA. [Gesch, Russell W.] USDA ARS, NCSCRL, Morris, MN 56267 USA. [Hatfield, Jerry L.] USDA ARS, NLAE, Ames, IA 50011 USA. [Kiniry, James R.] USDA ARS, GSWRL, Temple, TX 76502 USA. [Long, Daniel S.] USDA ARS, CPCRC, Pendleton, OR 97801 USA. RP Oblath, EA (reprint author), USDA ARS, NCAUR, 1815 N Univ St, Peoria, IL 61604 USA. EM emily.oblath@ars.usda.gov; terry.isbell@ars.usda.gov; mark.berhow@ars.usda.gov; brett.allen@ars.usda.gov; david.archer@ars.usda.gov; jbrown@uidaho.edu; russ.gesch@ars.usda.gov; jerry.hatfield@ars.usda.gov; jay.jabro@ars.usda.gov; jim.kiniry@ars.usda.gov; dan.long@ars.usda.gov FU USDA National Institute of Food and Agriculture (NIFA) [2012-10008-19727] FX The authors would like to thank Linda K. Manthey for technical assistance. This work was supported by Research Grant Award No. 2012-10008-19727 from the USDA National Institute of Food and Agriculture (NIFA). Seed for B. carinata genotypes was provided under an unfunded cooperative agreement with Agrisoma Biosciences, Inc. NR 34 TC 0 Z9 0 U1 18 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD OCT 30 PY 2016 VL 89 BP 52 EP 58 DI 10.1016/j.indcrop.2016.03.022 PG 7 WC Agricultural Engineering; Agronomy SC Agriculture GA DT1GZ UT WOS:000381231000008 ER PT J AU Hagler, JR Tassone, EE Coffelt, TA Lipka, AE AF Hagler, James R. Tassone, Erica E. Coffelt, Terry A. Lipka, Alexander E. TI Host plant preference of Lygus hesperus exposed to three desert-adapted industrial crops SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Centrapalus pauciflorus; Parthenium argretatum; Physaria fendleri; Choice test; Oviposition preference; Feeding preference ID ALFALFA TRAP CROPS; ORGANIC STRAWBERRIES; VERNONIA-GALAMENSIS; MIRIDAE; COTTON; HEMIPTERA; BUG; HETEROPTERA; ABSCISSION; MOVEMENT AB The desert-adapted crops vernonia (Centrapalus pauciflorus), lesquerella (Physaria fendleri), and camelina (Camelina sativa) are being grown in the arid southwestern USA as potential feedstock for biofuel and/or other environmentally friendly products. A plant feeding choice test was conducted to determine the relative attractiveness of these three "new" crops to a possible insect pest, Lygus hesperus Knight. Adult L. hesperus were readily observed feeding or resting on the flowering structures of each plant type, but they were seen most often on vernonia and least often on camelina. Lygus hesperus readily deposited their eggs on each plant species, but again, the greatest amount of egg deposition was found on vernonia and the least on camelina. These studies indicate that L. hesperus might pose a threat to the production of these new crops. Moreover, the commercial expansion of these crops could significantly alter the population dynamics of the existing arthropod community. New challenges for managing this pest during regional crop production changes are discussed. Published by Elsevier B.V. C1 [Hagler, James R.; Tassone, Erica E.; Coffelt, Terry A.] USDA ARS, Arid Land Agr Res Ctr, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. [Lipka, Alexander E.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. RP Hagler, JR (reprint author), USDA ARS, Arid Land Agr Res Ctr, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. EM james.hagler@ars.usda.gov NR 31 TC 0 Z9 0 U1 15 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD OCT 30 PY 2016 VL 89 BP 363 EP 367 DI 10.1016/j.indcrop.2016.05.041 PG 5 WC Agricultural Engineering; Agronomy SC Agriculture GA DT1GZ UT WOS:000381231000046 ER PT J AU Schillinger, WF Werner, SJ AF Schillinger, William F. Werner, Scott J. TI Horned lark damage to pre-emerged canola seedlings SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Biodiesel; Eremophilia alperestis; Horned lark; Canola seedlings; Crop damage ID CROPS AB Winter canola (Brassica napus L.) is considered the most promising domestically-produced oilseed feedstock for biodiesel production and for diversifying wheat (Triticum aestivum L.)-based cropping systems in the Inland Pacific Northwest, USA. Winter canola field experiments conducted in east-central Washington were completely destroyed, and commercial fields were damaged or destroyed, over several years by large flocks of horned larks (Eremophilia alperestis L) that ate the cotyledon leaves of pre-emerged and newly-emerged seedlings. Numerous control methods were attempted in field experiments, including laying bird netting over the entire experiment, placement of a life-size predator decoy in a field experiment, loud propane-powered cannon blasts, and mixing garlic with canola seed before planting followed by spraying garlic water on the soil surface. None of the attempted control methods were successful. This is the first report in the literature of horned lark damage to pre-emerged and newly-emerged canola seedlings. We discuss questions relevant to our novel account as well as potential abatement using falcons and non-toxic chemical repellents for the protection of industrial canola crops associated with horned lark depredation. (C) 2016 Elsevier B.V. All rights reserved. C1 [Schillinger, William F.] Washington State Univ, Dept Crop & Soil Sci, Dryland Res Stn, 781 E Expt Stn Rd, Lind, WA 99341 USA. [Werner, Scott J.] USDA APHIS Wildlife Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. RP Schillinger, WF (reprint author), Washington State Univ, Dept Crop & Soil Sci, Dryland Res Stn, 781 E Expt Stn Rd, Lind, WA 99341 USA. EM william.schillinger@wsu.edu FU WSU Agricultural Research Center through Hatch Project [0250]; Washington State Legislature FX Support was provided by the WSU Agricultural Research Center through Hatch Project 0250 and by the WSU Oilseed Cropping Systems Project through direct funding from the Washington State Legislature. The authors thank WSU research technicians John Jacobsen, Bruce Sauer, Steve Schofstoll, and Brian Fode for their excellent assistance with field experiments. NR 12 TC 0 Z9 0 U1 10 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD OCT 30 PY 2016 VL 89 BP 465 EP 467 DI 10.1016/j.indcrop.2016.05.045 PG 3 WC Agricultural Engineering; Agronomy SC Agriculture GA DT1GZ UT WOS:000381231000058 ER PT J AU Day, K Waite, LL Alonso, A Irvin, MR Zhi, D Thibeault, KS Aslibekyan, S Hidalgo, B Borecki, IB Ordovas, JM Arnett, DK Tiwari, HK Absher, DM AF Day, Kenneth Waite, Lindsay L. Alonso, Arnald Irvin, Marguerite R. Zhi, Degui Thibeault, Krista S. Aslibekyan, Stella Hidalgo, Bertha Borecki, Ingrid B. Ordovas, Jose M. Arnett, Donna K. Tiwari, Hemant K. Absher, Devin M. TI Heritable DNA Methylation in CD4(+) Cells among Complex Families Displays Genetic and Non-Genetic Effects SO PLOS ONE LA English DT Article ID TRANSGENERATIONAL EPIGENETIC INHERITANCE; ALLELE-SPECIFIC METHYLATION; EPIGENOME-WIDE ASSOCIATION; QUANTITATIVE TRAIT LOCUS; LIPID-LOWERING DRUGS; HUMAN LIFE-COURSE; DIET NETWORK; FENOFIBRATE TREATMENT; GERMLINE EPIGENOME; MULTIPLE TISSUES AB DNA methylation at CpG sites is both heritable and influenced by environment, but the relative contributions of each to DNA methylation levels are unclear. We conducted a heritability analysis of CpG methylation in human CD4+ cells across 975 individuals from 163 families in the Genetics of Lipid-lowering Drugs and Diet Network (GOLDN). Based on a broad-sense heritability (H-2) value threshold of 0.4, we identified 20,575 highly heritable CpGs among the 174,445 most variable autosomal CpGs (SD > 0.02). Tests for associations of heritable CpGs with genotype at 2,145,360 SNPs using 717 of 975 individuals showed that similar to 74% were cis-meQTLs (< 1 Mb away from the CpG), 6% of CpGs exhibited trans-meQTL associations (> 1 Mb away from the CpG or located on a different chromosome), and 20% of CpGs showed no strong significant associations with genotype (based on a p-value threshold of 1e-7). Genes proximal to the genotype independent heritable CpGs were enriched for functional terms related to regulation of T cell activation. These CpGs were also among those that distinguished T cells from other blood cell lineages. Compared to genes proximal to meQTL-associated heritable CpGs, genotype independent heritable CpGs were moderately enriched in the same genomic regions that escape erasure during primordial germ cell development and could carry potential for generational transmission. C1 [Day, Kenneth; Waite, Lindsay L.; Alonso, Arnald; Thibeault, Krista S.; Absher, Devin M.] HudsonAlpha Inst Biotechnol, Huntsville, AL 35806 USA. [Waite, Lindsay L.; Zhi, Degui; Hidalgo, Bertha; Tiwari, Hemant K.] Univ Alabama Birmingham, Sch Publ Hlth, Dept Biostat, Birmingham, AL 35294 USA. [Irvin, Marguerite R.; Aslibekyan, Stella; Arnett, Donna K.] Univ Alabama Birmingham, Sch Publ Hlth, Dept Epidemiol, Birmingham, AL 35294 USA. [Borecki, Ingrid B.] Washington Univ, Div Stat Genom, St Louis, MO USA. [Ordovas, Jose M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Ordovas, Jose M.] IMDEA Food, Madrid, Spain. [Alonso, Arnald] Vall dHebron Hosp Res Inst, Rheumatol Res Grp, Barcelona, Spain. [Arnett, Donna K.] Univ Kentucky, Coll Publ Hlth, Lexington, KY USA. RP Absher, DM (reprint author), HudsonAlpha Inst Biotechnol, Huntsville, AL 35806 USA. EM dabsher@hudsonalpha.org FU National Institutes of Health National Heart, Blood and Lung Institute [R01 HL104135-01]; U.S. Public Health Service Resource Grant from the National Center for Research Resources [RR03655] FX This work was supported by the National Institutes of Health National Heart, Blood and Lung Institute (R01 HL104135-01: http://www.nhbi.nih.gov) to DMA and DKA. Some of the results in this paper were obtained by using the software package S.A.G.E., which was supported by a U.S. Public Health Service Resource Grant (RR03655) from the National Center for Research Resources. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 75 TC 0 Z9 0 U1 0 U2 0 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD OCT 28 PY 2016 VL 11 IS 10 DI 10.1371/journal.pone.0165488 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EE3YA UT WOS:000389537000024 ER PT J AU Schicker, RS Rossow, J Eckel, S Fisher, N Bidol, S Tatham, L Matthews-Greer, J Sohner, K Bowman, AS Avrill, J Forshey, T Blanton, L Davis, CT Schiltz, J Skorupski, S Berman, L Jang, YH Bresee, JS Lindstrom, S Trock, SC Wentworth, D Fry, AM de Fijter, S Signs, K DiOrio, M Olsen, SJ Biggerstaff, M AF Schicker, Rebekah S. Rossow, John Eckel, Seth Fisher, Nicolas Bidol, Sally Tatham, Lilith Matthews-Greer, Janice Sohner, Kevin Bowman, Andrew S. Avrill, James Forshey, Tony Blanton, Lenee Davis, C. Todd Schiltz, John Skorupski, Susan Berman, LaShondra Jang, Yunho Bresee, Joseph S. Lindstrom, Stephen Trock, Susan C. Wentworth, David Fry, Alicia M. de Fijter, Sietske Signs, Kimberly DiOrio, Mary Olsen, Sonja J. Biggerstaff, Matthew TI Outbreak of Influenza A(H3N2) Variant Virus Infections Among Persons Attending Agricultural Fairs Housing Infected Swine - Michigan and Ohio, July-August 2016 SO MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT LA English DT Article ID UNITED-STATES; PIGS; H3N2 C1 [Schicker, Rebekah S.] CDC, Epidem Intelligence Serv, Atlanta, GA 30333 USA. [Schicker, Rebekah S.; Rossow, John; Blanton, Lenee; Davis, C. Todd; Berman, LaShondra; Jang, Yunho; Bresee, Joseph S.; Lindstrom, Stephen; Trock, Susan C.; Wentworth, David; Fry, Alicia M.; Olsen, Sonja J.; Biggerstaff, Matthew] CDC, Influenza Div, Natl Ctr Immunizat & Resp Dis, Atlanta, GA 30333 USA. [Rossow, John] CDC, Epidemiol Elective Program, Div Sci Educ & Profess Dev, Ctr Surveillance Epidemiol & Lab Serv, Atlanta, GA 30333 USA. [Eckel, Seth; Bidol, Sally; Matthews-Greer, Janice; Signs, Kimberly] Michigan Dept Hlth & Human Serv, Lansing, MI USA. [Fisher, Nicolas; Tatham, Lilith; Sohner, Kevin; de Fijter, Sietske; DiOrio, Mary] Ohio Dept Hlth, Columbus, OH 43266 USA. [Bowman, Andrew S.] Ohio State Univ, Coll Vet Med, Columbus, OH 43210 USA. [Avrill, James] Michigan Dept Agr & Rural Dev, Anim Ind Div, Lansing, MI USA. [Forshey, Tony] Ohio Dept Agr, Div Anim Hlth, Reynoldsburg, OH USA. [Schiltz, John] USDA, Natl Vet Serv Labs, Washington, DC 20250 USA. [Skorupski, Susan] USDA, Anim & Plant Hlth Inspect Serv, Washington, DC 20250 USA. RP Schicker, RS (reprint author), CDC, Epidem Intelligence Serv, Atlanta, GA 30333 USA.; Schicker, RS (reprint author), CDC, Influenza Div, Natl Ctr Immunizat & Resp Dis, Atlanta, GA 30333 USA. EM rschicker@cdc.gov NR 9 TC 1 Z9 1 U1 0 U2 0 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 0149-2195 EI 1545-861X J9 MMWR-MORBID MORTAL W JI MMWR-Morb. Mortal. Wkly. Rep. PD OCT 28 PY 2016 VL 65 IS 42 BP 1157 EP 1160 PG 4 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA EA5EZ UT WOS:000386643500001 PM 27787493 ER PT J AU Arbizu, CI Ellison, SL Senalik, D Simon, PW Spooner, DM AF Arbizu, Carlos I. Ellison, Shelby L. Senalik, Douglas Simon, Philipp W. Spooner, David M. TI Genotyping-by-sequencing provides the discriminating power to investigate the subspecies of Daucus carota (Apiaceae) SO BMC EVOLUTIONARY BIOLOGY LA English DT Article DE Carrot; Daucus carota; Genotyping-by-sequencing (GBS); Phylogeny; Single nucleotide polymorphisms (SNPs) ID FRAGMENT-LENGTH-POLYMORPHISMS; GUTTATUS COMPLEX APIACEAE; MISSING DATA; POPULATION-STRUCTURE; GENETIC-STRUCTURE; PHYLOGENETIC ANALYSIS; MITOCHONDRIAL GENOMES; INFERRING PHYLOGENY; MOLECULAR PHYLOGENY; L. GERMPLASM AB Background: The majority of the subspecies of Daucus carota have not yet been discriminated clearly by various molecular or morphological methods and hence their phylogeny and classification remains unresolved. Recent studies using 94 nuclear orthologs and morphological characters, and studies employing other molecular approaches were unable to distinguish clearly many of the subspecies. Fertile intercrosses among traditionally recognized subspecies are well documented. We here explore the utility of single nucleotide polymorphisms (SNPs) generated by genotyping-by-sequencing (GBS) to serve as an effective molecular method to discriminate the subspecies of the D. carota complex. Results: We used GBS to obtain SNPs covering all nine Daucus carota chromosomes from 162 accessions of Daucus and two related genera. To study Daucus phylogeny, we scored a total of 10,814 or 38,920 SNPs with a maximum of 10 or 30 % missing data, respectively. To investigate the subspecies of D. carota, we employed two data sets including 150 accessions: (i) rate of missing data 10 % with a total of 18,565 SNPs, and (ii) rate of missing data 30 %, totaling 43,713 SNPs. Consistent with prior results, the topology of both data sets separated species with 2n = 18 chromosome from all other species. Our results place all cultivated carrots (D. carota subsp. sativus) in a single clade. The wild members of D. carota from central Asia were on a clade with eastern members of subsp. sativus. The other subspecies of D. carota were in four clades associated with geographic groups: (1) the Balkan Peninsula and the Middle East, (2) North America and Europe, (3) North Africa exclusive of Morocco, and (4) the Iberian Peninsula and Morocco. Daucus carota subsp. maximus was discriminated, but neither it, nor subsp. gummifer (defined in a broad sense) are monophyletic. Conclusions: Our study suggests that (1) the morphotypes identified as D. carota subspecies gummifer (as currently broadly circumscribed), all confined to areas near the Atlantic Ocean and the western Mediterranean Sea, have separate origins from sympatric members of other subspecies of D. carota, (2) D. carota subsp. maximus, on two clades with some accessions of subsp. carota, can be distinguished from each other but only with poor morphological support, (3) D. carota subsp. capillifolius, well distinguished morphologically, is an apospecies relative to North African populations of D. carota subsp. carota, (4) the eastern cultivated carrots have origins closer to wild carrots from central Asia than to western cultivated carrots, and (5) large SNP data sets are suitable for species-level phylogenetic studies in Daucus. C1 [Arbizu, Carlos I.; Ellison, Shelby L.; Senalik, Douglas; Simon, Philipp W.; Spooner, David M.] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Senalik, Douglas; Simon, Philipp W.; Spooner, David M.] Univ Wisconsin, USDA ARS, Vegetable Crops Res Unit, 1575 Linden Dr, Madison, WI 53706 USA. RP Spooner, DM (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA.; Spooner, DM (reprint author), Univ Wisconsin, USDA ARS, Vegetable Crops Res Unit, 1575 Linden Dr, Madison, WI 53706 USA. EM david.spooner@ars.usda.gov FU USDA Agricultural Research Service; National Council of Science and Technology of Peru (Concytec) FX This work is supported by the USDA Agricultural Research Service. C. Arbizu is partly funded by the National Council of Science and Technology of Peru (Concytec). NR 127 TC 0 Z9 0 U1 13 U2 13 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2148 J9 BMC EVOL BIOL JI BMC Evol. Biol. PD OCT 28 PY 2016 VL 16 AR 234 DI 10.1186/s12862-016-0806-x PG 16 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA EA0XS UT WOS:000386313500002 PM 27793080 ER PT J AU Brocca, L Pellarin, T Crow, WT Ciabatta, L Massari, C Ryu, D Su, CH Rudiger, C Kerr, Y AF Brocca, Luca Pellarin, Thierry Crow, Wade T. Ciabatta, Luca Massari, Christian Ryu, Dongryeol Su, Chun-Hsu Rudiger, Christoph Kerr, Yann TI Rainfall estimation by inverting SMOS soil moisture estimates: A comparison of different methods over Australia SO JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES LA English DT Article DE soil moisture; rainfall; remote sensing; SMOS ID TIME SATELLITE PRECIPITATION; SOUTHEAST AUSTRALIA; ANALYSIS TMPA; LAND-SURFACE; DATA-SETS; AMSR-E; SCALE; ASSIMILATION; RETRIEVALS; PRODUCTS AB Remote sensing of soil moisture has reached a level of maturity and accuracy for which the retrieved products can be used to improve hydrological and meteorological applications. In this study, the soil moisture product from the Soil Moisture and Ocean Salinity (SMOS) satellite is used for improving satellite rainfall estimates obtained from the Tropical Rainfall Measuring Mission multisatellite precipitation analysis product (TMPA) using three different bottom up techniques: SM2RAIN, Soil Moisture Analysis Rainfall Tool, and Antecedent Precipitation Index Modification. The implementation of these techniques aims at improving the well-known top down rainfall estimate derived from TMPA products (version 7) available in near real time. Ground observations provided by the Australian Water Availability Project are considered as a separate validation data set. The three algorithms are calibrated against the gauge-corrected TMPA reanalysis product, 3B42, and used for adjusting the TMPA real-time product, 3B42RT, using SMOS soil moisture data. The study area covers the entire Australian continent, and the analysis period ranges from January 2010 to November 2013. Results show that all the SMOS-based rainfall products improve the performance of 3B42RT, even at daily time scale (differently from previous investigations). The major improvements are obtained in terms of estimation of accumulated rainfall with a reduction of the root-mean-square error of more than 25%. Also, in terms of temporal dynamic (correlation) and rainfall detection (categorical scores) the SMOS-based products provide slightly better results with respect to 3B42RT, even though the relative performance between the methods is not always the same. The strengths and weaknesses of each algorithm and the spatial variability of their performances are identified in order to indicate the ways forward for this promising research activity. Results show that the integration of bottom up and top down approaches has the potential to improve the quality of near-real-time rainfall estimates from remote sensing in the near future. C1 [Brocca, Luca; Ciabatta, Luca; Massari, Christian] CNR, Res Inst Geohydrol Protect, Perugia, Italy. [Pellarin, Thierry] Univ Grenoble Alpes, CNRS, LTHE, Grenoble, France. [Crow, Wade T.] Hydrol & Remote Sensing Lab, USDA ARS, Beltsville, MD USA. [Ryu, Dongryeol; Su, Chun-Hsu] Univ Melbourne, Dept Infrastruct Engn, Melbourne, Vic, Australia. [Rudiger, Christoph] Monash Univ, Dept Civil Engn, Clayton, Vic, Australia. [Kerr, Yann] CNRS, CNES, IRD, Ctr Etudes Spati Biosphere UPS, Toulouse, France. RP Brocca, L (reprint author), CNR, Res Inst Geohydrol Protect, Perugia, Italy. EM luca.brocca@irpi.cnr.it RI Brocca, Luca/F-2854-2010 OI Brocca, Luca/0000-0002-9080-260X FU European Space Agency SMOS+rainfall project [4000114738/15/I-SBo]; Centre National d'Etudes Spatiales TOSCA FX We would like to acknowledge the Australian Bureau of Meteorology for providing daily rainfall data (accessed online via http://www.bom.gov.au/jsp/awap/rain/archive_recal.jsp), the support of the European Space Agency SMOS+rainfall project (contract 4000114738/15/I-SBo), and of the Centre National d'Etudes Spatiales TOSCA. SMOS L3SM product was provided by the Centre Aval de Traitement des Donnees SMOS (CATDS), developed by CNES in collaboration with CESBIO and IFREMER. All the input data sets used in the paper are available for free through public websites. The four rainfall products estimated/corrected by using SMOS soil moisture data are available upon request from the corresponding author. NR 56 TC 1 Z9 1 U1 8 U2 8 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-897X EI 2169-8996 J9 J GEOPHYS RES-ATMOS JI J. Geophys. Res.-Atmos. PD OCT 27 PY 2016 VL 121 IS 20 BP 12062 EP 12079 DI 10.1002/2016JD025382 PG 18 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA EC7CJ UT WOS:000388293100034 ER PT J AU Spackman, E Cardona, C Munoz-Aguayo, J Fleming, S AF Spackman, Erica Cardona, Carol Munoz-Aguayo, Jeannette Fleming, Susan TI Successes and Short Comings in Four Years of an International External Quality Assurance Program for Animal Influenza Surveillance SO PLoS One LA English DT Article ID LINKED-IMMUNOSORBENT-ASSAY; VIRUS; PROFICIENCY; ANTIBODIES; DIAGNOSIS AB The US National institutes of Health-Centers of Excellence for Influenza Research and Surveillance is a research consortium that funds numerous labs worldwide to conduct influenza A surveillance in diverse animal species. There is no harmonization of testing procedures among these labs; therefore an external quality assurance (EQA) program was implemented to evaluate testing accuracy among labs in the program in 2012. Accurate detection of novel influenza A variants is crucial because of the broad host range and potentially high virulence of the virus in diverse species. Two molecular detection sample sets and 2 serology sample sets (one with avian origin isolates, and one with mammalian origin isolates each) were made available at approximately six month intervals. Participating labs tested the material in accordance with their own protocols. During a five year period a total of 41 labs from 23 countries ordered a total of 132 avian molecular, 121 mammalian molecular and 90 serology sample sets. Testing was completed by 111 individuals. Detection of type A influenza by RT-PCR was reliable with a pass rate (80% or greater agreement with expected results) of 86.6% for avian and 86.2% for mammalian origin isolates. However, identification of subtype by RT-PCR was relatively poor with 54.1% and 75.9% accuracy for avian and mammalian influenza isolates respectively. Serological testing had an overall pass rate of 86.9% and 22/23 labs used commercial ELISA kits. Based on the results of this EQA program six labs modified their procedures to improve accuracy and one lab identified an unknown equipment problem. These data represent the successful implementation of an international EQA program for an infectious disease; insights into the logistics and test design are also discussed. C1 [Spackman, Erica] ARS, Exot & Emerging Avian Viral Dis Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr,USDA, Athens, GA 30605 USA. [Cardona, Carol; Munoz-Aguayo, Jeannette; Fleming, Susan] Univ Minnesota, Dept Vet & Biomed Sci, St Paul, MN 55108 USA. RP Spackman, E (reprint author), ARS, Exot & Emerging Avian Viral Dis Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr,USDA, Athens, GA 30605 USA. EM Erica.Spackman@ars.usda.gov FU US Department of Agriculture, ARS CRIS Project [6040-32000-063-00D]; National Institute of Allergy and Infectious Diseases, National Institutes of Health [AAI12004-001-00001]; Minnesota Center for Excellence in Influenza Research and Surveillance [HHSN266200700007] FX Support was provided by US Department of Agriculture, ARS CRIS Project 6040-32000-063-00D and by federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, under IAA No. AAI12004-001-00001 and the Minnesota Center for Excellence in Influenza Research and Surveillance HHSN266200700007. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; Support was provided by US Department of Agriculture, ARS CRIS Project 6040-32000063-00D and by federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, under IAA No. AAI12004-001-00001 and the Minnesota Center for Excellence in Influenza Research and Surveillance HHSN266200700007. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. NR 16 TC 0 Z9 0 U1 1 U2 1 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD OCT 27 PY 2016 VL 11 IS 10 AR e0164261 DI 10.1371/journal.pone.0164261 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EE4VY UT WOS:000389604900015 PM 27788155 ER PT J AU Paneru, B Al-Tobasei, R Palti, Y Wiens, GD Salem, M AF Paneru, Bam Al-Tobasei, Rafet Palti, Yniv Wiens, Gregory D. Salem, Mohamed TI Differential expression of long non-coding RNAs in three genetic lines of rainbow trout in response to infection with Flavobacterium psychrophilum SO SCIENTIFIC REPORTS LA English DT Article ID NEUTROPHIL ELASTASE; ONCORHYNCHUS-MYKISS; INNATE IMMUNITY; UP-REGULATION; CUTTING EDGE; IN-VIVO; ACTIVATION; CELLS; PATHWAY; PROTEIN AB Bacterial cold-water disease caused by Flavobacterium psychrophilum is one of the major causes of mortality of salmonids. Three genetic lines of rainbow trout designated as ARS-Fp-R (resistant), ARS-Fp-C (control) and ARS-Fp-S (susceptible) have significant differences in survival rate following F. psychrophilum infection. Previous study identified transcriptome differences of immune-relevant protein-coding genes at basal and post infection levels among these genetic lines. Using RNA-Seq approach, we quantified differentially expressed (DE) long non-coding RNAs (lncRNAs) in response to F. psychrophilum challenge in these genetic lines. Pairwise comparison between genetic lines and different infection statuses identified 556 DE lncRNAs. A positive correlation existed between the number of the differentially regulated lncRNAs and that of the protein-coding genes. Several lncRNAs showed strong positive and negative expression correlation with their overlapped, neighboring and distant immune related protein-coding genes including complement components, cytokines, chemokines and several signaling molecules involved in immunity. The correlated expressions and genome-wide co-localization suggested that some lncRNAs may be involved in regulating immune-relevant protein-coding genes. This study provides the first evidence of lncRNA-mediated regulation of the anti-bacterial immune response in a commercially important aquaculture species and will likely help developing new genetic markers for rainbow trout disease resistance. C1 [Paneru, Bam; Salem, Mohamed] Middle Tennessee State Univ, Dept Biol, Murfreesboro, TN 37132 USA. [Paneru, Bam; Salem, Mohamed] Middle Tennessee State Univ, Mol Biosci Program, Murfreesboro, TN 37132 USA. [Al-Tobasei, Rafet; Salem, Mohamed] Middle Tennessee State Univ, Computat Sci Program, Murfreesboro, TN 37132 USA. [Palti, Yniv; Wiens, Gregory D.] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV 25430 USA. RP Salem, M (reprint author), Middle Tennessee State Univ, Dept Biol, Murfreesboro, TN 37132 USA.; Salem, M (reprint author), Middle Tennessee State Univ, Mol Biosci Program, Murfreesboro, TN 37132 USA.; Salem, M (reprint author), Middle Tennessee State Univ, Computat Sci Program, Murfreesboro, TN 37132 USA. EM Mohamed.salem@mtsu.edu FU Agricultural Research Service CRIS Project "Integrated Approaches for Improving Aquatic Animal Health in Cool and Cold Water Aquaculture" [1930-32000-005]; CRIS Project "Utilization of Genomics for Improving Production Traits in Cool and Cold Water Aquaculture" [1930-32000-009]; Agriculture and Food Research Initiative from the USDA National Institute of Food and Agriculture [2012-67015-30217, 2014-67015-21602] FX We acknowledge technical contributions from Travis Moreland and Joel Caren. This work was supported by Agricultural Research Service CRIS Project 1930-32000-005 "Integrated Approaches for Improving Aquatic Animal Health in Cool and Cold Water Aquaculture," CRIS Project 1930-32000-009 "Utilization of Genomics for Improving Production Traits in Cool and Cold Water Aquaculture" and Agriculture and Food Research Initiative competitive grants no. 2012-67015-30217 (to Gregory D. Wiens) and No. 2014-67015-21602 (to Mohamed Salem) from the USDA National Institute of Food and Agriculture. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US, Department of Agriculture. USDA is an equal opportunity employer. NR 66 TC 2 Z9 2 U1 7 U2 7 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD OCT 27 PY 2016 VL 6 AR 36032 DI 10.1038/srep36032 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EA0XK UT WOS:000386312000001 PM 27786264 ER PT J AU Lerch, AP Pfammatter, JA Bentz, BJ Raffa, KF AF Lerch, Andrew P. Pfammatter, Jesse A. Bentz, Barbara J. Raffa, Kenneth F. TI Mountain Pine Beetle Dynamics and Reproductive Success in Post-Fire Lodgepole and Ponderosa Pine Forests in Northeastern Utah SO PLOS ONE LA English DT Article ID FIRE SURROGATE TREATMENTS; BARK BEETLE; TREE MORTALITY; PRESCRIBED FIRE; SPATIOTEMPORAL PATTERNS; NORTHERN ARIZONA; ROCKY-MOUNTAINS; RESIN DEFENSES; ATTACKS; OUTBREAKS AB Fire injury can increase tree susceptibility to some bark beetles (Curculionidae, Scolytinae), but whether wildfires can trigger outbreaks of species such as mountain pine beetle (Den-droctonus ponderosae Hopkins) is not well understood. We monitored 1173 lodgepole (Pinus contorta var. latifolia Doug.) and 599 ponderosa (Pinus ponderosa Doug. ex Law) pines for three years post-wildfire in the Uinta Mountains of northeastern Utah in an area with locally endemic mountain pine beetle. We examined how the degree and type of fire injury influenced beetle attacks, brood production, and subsequent tree mortality, and related these to beetle population changes over time. Mountain pine beetle population levels were high the first two post-fire years in lodgepole pine, and then declined. In ponderosa pine, populations declined each year after initial post-fire sampling. Compared to trees with strip or failed attacks, mass attacks occurred on trees with greater fire injury, in both species. Overall, a higher degree of damage to crowns and boles was associated with higher attack rates in ponderosa pines, but additional injury was more likely to decrease attack rates in lodgepole pines. In lodgepole pine, attacks were initially concentrated on fireinjured trees, but during subsequent years beetles attacked substantial numbers of uninjured trees. In ponderosa pine, attacks were primarily on injured trees each year, although these stands were more heavily burned and had few uninjured trees. In total, 46% of all lodgepole and 56% of ponderosa pines underwent some degree of attack. Adult brood emergence within caged bole sections decreased with increasing bole char in lodgepole pine but increased in ponderosa pine, however these relationships did not scale to whole trees. Mountain pine beetle populations in both tree species four years post-fire were substantially lower than the year after fire, and wildfire did not result in population outbreaks. C1 [Lerch, Andrew P.; Raffa, Kenneth F.] Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA. [Pfammatter, Jesse A.] Univ Wisconsin, Dept Neurosci, Madison, WI USA. [Bentz, Barbara J.] USDA, Forest Serv, Rocky Mt Res Stn, Logan, UT USA. RP Lerch, AP (reprint author), Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA. EM lerch.andrew@gmail.com FU USDA Forest Service, State and Private Forestry, Forest Health Monitoring [INT-F-08-01] FX USDA Forest Service, State and Private Forestry, Forest Health Monitoring, INT-F-08-01; BJB KFR. NR 50 TC 0 Z9 0 U1 3 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD OCT 26 PY 2016 VL 11 IS 10 AR e0164738 DI 10.1371/journal.pone.0164738 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EE4VE UT WOS:000389602800047 PM 27783632 ER PT J AU Ravi, S Sharratt, BS Li, JR Olshevski, S Meng, ZJ Zhang, JG AF Ravi, Sujith Sharratt, Brenton S. Li, Junran Olshevski, Stuart Meng, Zhongju Zhang, Jianguo TI Particulate matter emissions from biochar-amended soils as a potential tradeoff to the negative emission potential SO SCIENTIFIC REPORTS LA English DT Article ID THRESHOLD FRICTION VELOCITY; CLIMATE-CHANGE; CARBON; REMOVAL; PARTICLES; BIOFILTER; BLACK; WIND AB Novel carbon sequestration strategies such as large-scale land application of biochar may provide sustainable pathways to increase the terrestrial storage of carbon. Biochar has a long residence time in the soil and hence comprehensive studies are urgently needed to quantify the environmental impacts of large-scale biochar application. In particular, black carbon emissions from soils amended with biochar may counteract the negative emission potential due to the impacts on air quality, climate, and biogeochemical cycles. We investigated, using wind tunnel experiments, the particulate matter emission potential of a sand and two agriculturally important soils amended with different concentrations of biochar, in comparison to control soils. Our results indicate that biochar application considerably increases particulate emissions possibly by two mechanisms-the accelerated emission of fine biochar particles and the generation and emission of fine biochar particles resulting from abrasion of large biochar particles by sand grains. Our study highlights the importance of considering the background soil properties (e.g., texture) and geomorphological processes (e.g., aeolian transport) for biochar-based carbon sequestration programs. C1 [Ravi, Sujith; Olshevski, Stuart] Temple Univ, Dept Earth & Environm Sci, Philadelphia, PA 19122 USA. [Sharratt, Brenton S.; Meng, Zhongju; Zhang, Jianguo] USDA ARS, Northwest Sustainable Agroecosyst Res, Pullman, WA 99164 USA. [Li, Junran] Univ Tulsa, Dept Geosci, Tulsa, OK 74104 USA. [Meng, Zhongju] Inner Mongolia Agr Univ, Coll Desert Sci & Engn, Hohhot 010019, Inner Mongolia, Peoples R China. [Zhang, Jianguo] Northwest A&F Univ, Coll Nat Resources & Environm, Xianyang 712100, Shaanxi, Peoples R China. RP Ravi, S (reprint author), Temple Univ, Dept Earth & Environm Sci, Philadelphia, PA 19122 USA. EM sravi@temple.edu FU US National Science Foundation [EAR-1451518] FX S.R. and J.L. acknowledge partial support from the US National Science Foundation (EAR-1451518). *Identification of experimental apparatus or company sources is for information purposes only and does not imply endorsement by ARS, USDA. NR 34 TC 0 Z9 0 U1 14 U2 14 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD OCT 26 PY 2016 VL 6 AR 35984 DI 10.1038/srep35984 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ8HS UT WOS:000386112100001 PM 27782159 ER PT J AU Gilligan, T Huemer, P Wiesmair, B AF Gilligan, Todd Huemer, Peter Wiesmair, Benjamin TI Different continents, same species? Resolving the taxonomy of some Holarctic Ancylis Hubner (Lepidoptera: Tortricidae) SO ZOOTAXA LA English DT Article DE christiandiana; DNA barcoding; Enarmoniini; oregonensis; saliana ID GENITALIA; EVOLUTION AB Several species of Ancylis related to A. unguicella (Linnaeus) and A. geminana (Donovan) have been presumed by previous authors to be Holarctic. However, difficulty in identifying genitalic characters to define and separate these taxa has brought into question their true distribution and led to inconsistencies in their taxonomic treatment in Europe and North America. Here we use a combination of DNA barcode sequence data and morphology to resolve these taxonomic differences, determine the actual geographic range of these taxa, and describe three new species. In the A. unguicella group, only A. unguicella and A. uncella (Denis & Schiffermuller) are Holarctic in distribution. In the A. geminana group, none of the taxa are Holarctic in their distribution. Three species are described as new: A. christiandiana Huemer and Wiesmair, sp.n. (Austria, Germany); A. oregonensis Gilligan and Huemer, sp.n. (USA: Oregon); and A. saliana Gilligan and Huemer, sp.n. (USA: Florida). In addition, Ancylis carbonana Heinrich, syn.n., is synonymized with A. uncella; A. cuspidana (Treitschke), syn.rev., is synonymized with A. diminutana (Haworth); and A. diminuatana Kearfott, stat.rev., and A. subarcuana (Douglas), stat.rev., are raised from synonymy. C1 [Gilligan, Todd] APHIS, Identificat Technol Program, USDA, PPQ,S&T, 2301 Res Blvd,Suite 108, Ft Collins, CO 80526 USA. [Huemer, Peter; Wiesmair, Benjamin] Tiroler Landesmuseen Betriebgsges mbH, Nat Wissensch Sammlungen, Feldstr 11a, A-6020 Innsbruck, Austria. RP Gilligan, T (reprint author), APHIS, Identificat Technol Program, USDA, PPQ,S&T, 2301 Res Blvd,Suite 108, Ft Collins, CO 80526 USA. EM todd.m.gilligan@aphis.usda.gov; p.huemer@tiroler-landesmuseen.at; b.wiesmair@tiroler-landesmuseen.at FU Government of Canada; Ontario Ministry of Research and Innovation; NSERC; Promotion of Educational Policies, University and Research Department of the Autonomous Province of Bolzano-South Tyrol; Austrian Federal Ministry of Science, Research and Economics FX We are particularly grateful to Paul Hebert and his team at the Canadian Centre for DNA Barcoding (Guelph, Canada) who's sequencing work was enabled by funding from the Government of Canada to Genome Canada through the Ontario Genomics Institute. We are also grateful to the Ontario Ministry of Research and Innovation and to NSERC for their support of the BOLD informatics platform. PH is furthermore indebted to the Promotion of Educational Policies, University and Research Department of the Autonomous Province of Bolzano-South Tyrol for helping to fund the project "Genetic biodiversity archive-DNA barcoding of Lepidoptera of the central Alpine region (South, East and North Tyrol)", and to the Austrian Federal Ministry of Science, Research and Economics for funds received in the framework of ABOL (Austrian Barcode of Life). NR 47 TC 0 Z9 0 U1 2 U2 2 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD OCT 26 PY 2016 VL 4178 IS 3 BP 347 EP 370 DI 10.11646/zootaxa.4178.3.3 PG 24 WC Zoology SC Zoology GA DZ6YE UT WOS:000386007900003 PM 27811713 ER PT J AU Clarke, CR Hayes, BW Runde, BJ Markel, E Swingle, BM Vinatzer, BA AF Clarke, Christopher R. Hayes, Byron W. Runde, Brendan J. Markel, Eric Swingle, Bryan M. Vinatzer, Boris A. TI Comparative genomics of Pseudomonas syringae pathovar tomato reveals novel chemotaxis pathways associated with motility and plant pathogenicity SO PEERJ LA English DT Article DE Chemotaxis; Flagellin; Swimming motility; CheA; Swarming motility; Twitching motility; DC3000; Pto ID PV. TABACI 6605; TWITCHING MOTILITY; IV PILI; BIOFILM FORMATION; RHODOBACTER-SPHAEROIDES; BACTERIAL CHEMOTAXIS; SIGNAL-TRANSDUCTION; ROOT COLONIZATION; GENE-CLUSTER; CHEY GENES AB The majority of bacterial foliar plant pathogens must invade the apoplast of host plants through points of ingress, such as stomata or wounds, to replicate to high population density and cause disease. How pathogens navigate plant surfaces to locate invasion sites remains poorly understood. Many bacteria use chemical-directed regulation of flagellar rotation, a process known as chernotaxis, to move towards favorable environmental conditions. Chernotactic sensing of the plant surface is a potential mechanism through which foliar plant pathogens home in on wounds or stomata, but chernotactic systems in foliar plant pathogens are not well characterized. Comparative genomics of the plant pathogen Pseudomonas syringae pathovar tomato (Pto) implicated annotated chemotwds genes in the recent adaptations of one Pto lineage. We therefore characterized the chemosensory system of Pto. The Pto genome contains two primary chemotaxis gene clusters, che1 and che2. The che2 cluster is flanked by flagellar biosynthesis genes and similar to the canonical chemotwds gene clusters of other bacteria based on sequence and synteny. Disruption of the primary phosphorelay kinase gene of the che2 cluster, cheA2, eliminated all swimming and surface motility at 21 degrees C but not 28 degrees C for Pto. The chel cluster is located next to Type IV pili biosynthesis genes but disruption of cheA1 has no observable effect on twitching motility for Pto. Disruption of cheA2 also alters in planta fitness of the pathogen with strains lacking functional cheA2 being less fit in host plants but more fit in a non-host interaction. C1 [Clarke, Christopher R.; Hayes, Byron W.; Runde, Brendan J.; Vinatzer, Boris A.] Virginia Tech, Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. [Markel, Eric; Swingle, Bryan M.] USDA, Emerging Pests & Pathogens Res Unit, Robert W Holley Ctr Agr & Hlth, Ithaca, NY USA. [Swingle, Bryan M.] Sch Integrat Plant, Plant Pathol & Plant Microbe Biol Sect, Ithaca, NY USA. RP Clarke, CR (reprint author), Virginia Tech, Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. EM gtg681r@vt.edu FU NSF [IOS-0746501, IOS-1354215]; Virginia Agricultural Experiment Station; Hatch Program of the National Institute of Food and Agriculture, US Department of Agriculture; National Institute of Food and Agriculture postdoctoral research fellowship [2015-67012-22821] FX This work was supported by NSF IOS-0746501 and IOS-1354215 to BAV. Funding to BAV was also provided in part by the Virginia Agricultural Experiment Station and the Hatch Program of the National Institute of Food and Agriculture, US Department of Agriculture. CRC is supported by a National Institute of Food and Agriculture postdoctoral research fellowship (2015-67012-22821). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 58 TC 0 Z9 0 U1 10 U2 10 PU PEERJ INC PI LONDON PA 341-345 OLD ST, THIRD FLR, LONDON, EC1V 9LL, ENGLAND SN 2167-8359 J9 PEERJ JI PeerJ PD OCT 25 PY 2016 VL 4 AR e2570 DI 10.7717/peerj.2570 PG 24 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EA2AB UT WOS:000386392300002 PM 27812402 ER PT J AU Ramey, AM Spackman, E Kim-Torchetti, M DeLiberto, TJ AF Ramey, Andrew M. Spackman, Erica Kim-Torchetti, Mia DeLiberto, Thomas J. TI Weak support for disappearance and restricted emergence/persistence of highly pathogenic influenza A in North American waterfowl SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Letter ID WILD BIRDS; SOUTH-KOREA; VIRUSES; H5N8 C1 [Ramey, Andrew M.] US Geol Survey, Alaska Sci Ctr, Anchorage, AK 99508 USA. [Spackman, Erica] ARS, Southeast Poultry Res Lab, USDA, Athens, GA 30605 USA. [Kim-Torchetti, Mia] Vet Serv, Natl Vet Serv Labs, Anim & Plant Hlth Inspect Serv, USDA, Ames, IA 50010 USA. [DeLiberto, Thomas J.] Wildlife Serv, Natl Wildlife Res Ctr, Anim & Plant Hlth Inspect Serv, USDA, Ft Collins, CO 80521 USA. RP Ramey, AM (reprint author), US Geol Survey, Alaska Sci Ctr, Anchorage, AK 99508 USA. EM aramey@usgs.gov NR 10 TC 1 Z9 1 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD OCT 25 PY 2016 VL 113 IS 43 BP E6551 EP E6552 DI 10.1073/pnas.1614530113 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ7ZI UT WOS:000386087100003 PM 27791026 ER PT J AU Kushanov, FN Pepper, AE Yu, JZ Buriev, ZT Shermatov, SE Saha, S Ulloa, M Jenkins, JN Abdukarimov, A Abdurakhmonov, IY AF Kushanov, Fakhriddin N. Pepper, Alan E. Yu, John Z. Buriev, Zabardast T. Shermatov, Shukhrat E. Saha, Sukumar Ulloa, Mauricio Jenkins, Johnie N. Abdukarimov, Abdusattor Abdurakhmonov, Ibrokhim Y. TI Development, genetic mapping and QTL association of cotton PHYA, PHYB, and HY5-specific CAPS and dCAPS markers SO BMC GENETICS LA English DT Article DE Cotton; Phytochromes; CAPS and dCAPS; Linkage mapping; Fiber QTLs ID SINGLE NUCLEOTIDE POLYMORPHISMS; GOSSYPIUM-HIRSUTUM L.; B-BOX PROTEIN; ARABIDOPSIS-THALIANA; TETRAPLOID COTTON; MOLECULAR MARKERS; FIBER QUALITY; PHYTOCHROME B; UPLAND COTTON; EXPRESSION AB Background: Among SNP markers that become increasingly valuable in molecular breeding of crop plants are the CAPS and dCAPS markers derived from the genes of interest. To date, the number of such gene-based markers is small in polyploid crop plants such as allotetraploid cotton that has A-and D-sub-genomes. The objective of this study was to develop and map new CAPS and dCAPS markers for cotton developmental-regulatory genes that are important in plant breeding programs. Results: Gossypium hirsutum and G. barbadense, are the two cultivated allotetraploid cotton species. These have distinct fiber quality and other agronomic traits. Using comparative sequence analysis of characterized GSTs of the PHYA1, PHYB, and HY5 genes of G. hirsutum and G. barbadense one PHYA1-specific Mbo I/Dpn II CAPS, one PHYB-specific Alu I dCAPS, and one HY5-specific Hinf I dCAPS cotton markers were developed. These markers have successfully differentiated the two allotetraploid genomes (AD(1) and AD(2)) when tested in parental genotypes of 'Texas Marker-1' ('TM-1'), 'Pima 3-79' and their F-1 hybrids. The genetic mapping and chromosome substitution line-based deletion analyses revealed that PHYA1 gene is located in A-sub-genome chromosome 11, PHYB gene is in A-sub-genome chromosome 10, and HY5 gene is in D-sub-genome chromosome 24, on the reference 'TM-1' x 'Pima 3-79' RIL genetic map. Further, it was found that genetic linkage map regions containing phytochrome and HY5-specific markers were associated with major fiber quality and flowering time traits in previously published QTL mapping studies. Conclusion: This study detailed the genome mapping of three cotton phytochrome genes with newly developed CAPS and dCAPS markers. The proximity of these loci to fiber quality and other cotton QTL was demonstrated in two A-subgenome and one D-subgenome chromosomes. These candidate gene markers will be valuable for marker-assisted selection (MAS) programs to rapidly introgress G. barbadense phytochromes and/or HY5 gene (s) into G. hirsutum cotton genotypes or vice versa. C1 [Kushanov, Fakhriddin N.; Buriev, Zabardast T.; Shermatov, Shukhrat E.; Abdukarimov, Abdusattor; Abdurakhmonov, Ibrokhim Y.] Acad Sci Uzbek, Ctr Genom & Bioinformat, Univ St 2, Qibray Region 111215, Uzbekistan. [Pepper, Alan E.] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA. [Yu, John Z.] USDA ARS, Southern Plains Agr Res Ctr, 2881 F&B Rd, College Stn, TX 77843 USA. [Saha, Sukumar; Jenkins, Johnie N.] USDA ARS, Crop Sci Res Lab, Mississippi State, MS 39762 USA. [Ulloa, Mauricio] USDA ARS, Plant Stress & Germplasm Dev Res, 3810 4th St, Lubbock, TX 79415 USA. RP Abdurakhmonov, IY (reprint author), Acad Sci Uzbek, Ctr Genom & Bioinformat, Univ St 2, Qibray Region 111215, Uzbekistan. EM genomics@uzsci.net; ibrokhim.abdurakhmonov@genomics.uz FU Office of International Research Programs of the United States Department of Agriculture (USDA) under the USDA-Former Soviet Union [P121/P121B]; Academy of science of Uzbekistan [FA-F5-T030] FX This research was funded by the Office of International Research Programs of the United States Department of Agriculture (USDA) under the USDA-Former Soviet Union cooperation programs with the research Grant of P121/P121B to IYA, SS and AEP. The research is also funded by Academy of science of Uzbekistan with the local research grant of FA-F5-T030. NR 59 TC 0 Z9 0 U1 10 U2 10 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2156 J9 BMC GENET JI BMC Genet. PD OCT 24 PY 2016 VL 17 AR 141 DI 10.1186/s12863-016-0448-4 PG 11 WC Genetics & Heredity SC Genetics & Heredity GA DZ6OS UT WOS:000385982400001 PM 27776497 ER PT J AU Tillman, PG Cottrell, TE AF Tillman, P. Glynn Cottrell, Ted E. TI Density and Egg Parasitism of Stink Bugs (Hemiptera:Pentatomidae) in Elderberry and Dispersal Into Crops SO JOURNAL OF INSECT SCIENCE LA English DT Article DE Noncrop host plant; dispersal; cotton; Anastatus; Trissolcus ID HETEROPTERA-PENTATOMIDAE; HEMIPTERA-PENTATOMIDAE; ASHMEAD HYMENOPTERA; EUSCHISTUS-SERVUS; SOUTH-CAROLINA; PECAN ORCHARDS; FIELD EDGES; COTTON; PHEROMONE; AGGREGATION AB Chinavia hilaris (Say), Euschistus servus (Say), Euschistus tristigmus (Say), and Thyanta custator custator (F.) (Hemiptera: Pentatomidae) are serious pests of crops in the southeastern United States but little is known concerning their dispersal from noncrop hosts in woodlands into crops. This 2-yr study was conducted to investigate whether elderberry [Sambucus nigra subsp. canadensis (L.) R. Bolli] in woodlands serves as a source of stink bugs dispersing into adjacent crops and to examine parasitism of C. hilaris and E. servus eggs on this plant. Elderberry was a reproductive host for each of the four stink bug species; females oviposited on plants with subsequent nymphs feeding on elderberry and developing into adults. Anastatus mirabilis (Walsh & Riley) (Hymenoptera: Eupelmidae), Anastatus reduvii (Howard), and Trissolcus edessae Fouts (Hymenoptera: Scelionidae) were prevalent egg parasitoids of C. hilaris but A. reduvii was the prevalent parasitoid of E. servus. Newly developed stink bug adults were first detected on elderberry around mid-July. Then in late July and early August, as elderberry fruit senesced and cotton bolls became available, stink bugs began dispersing from elderberry into cotton based on recapture of stink bugs on cotton that had previously been marked on elderberry. In addition, in 2015, density of C. hilaris, E. servus, and E. tristigmus was higher in cotton with elderberry than in cotton without it. Over the study, economic threshold was reached for four of seven cotton fields. Elimination of elderberry in woodlands adjacent to cotton may be a viable management tactic for control of stink bugs in cotton. C1 [Tillman, P. Glynn] ARS, USDA, Crop Protect & Management Res Lab, 2747 Davis Rd, Tifton, GA 31793 USA. [Cottrell, Ted E.] Agr Res Serv, USDA, Southeastern Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. RP Tillman, PG (reprint author), ARS, USDA, Crop Protect & Management Res Lab, 2747 Davis Rd, Tifton, GA 31793 USA. EM Glynn.Tillman@ars.usda.gov; Ted.Cottrell@ars.usda.gov NR 41 TC 0 Z9 0 U1 7 U2 7 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1536-2442 EI 2250-2645 J9 J INSECT SCI JI J Insect Sci. PD OCT 23 PY 2016 VL 16 AR 106 DI 10.1093/jisesa/iew091 PG 14 WC Entomology SC Entomology GA EA6EP UT WOS:000386719300002 ER PT J AU Lin, CY Jakes, JE Donohoe, BS Ciesielski, PN Yang, HB Gleber, SC Vogt, S Ding, SY Peer, WA Murphy, AS McCann, MC Himmel, ME Tucker, MP Wei, H AF Lin, Chien-Yuan Jakes, Joseph E. Donohoe, Bryon S. Ciesielski, Peter N. Yang, Haibing Gleber, Sophie-Charlotte Vogt, Stefan Ding, Shi-You Peer, Wendy A. Murphy, Angus S. McCann, Maureen C. Himmel, Michael E. Tucker, Melvin P. Wei, Hui TI Directed plant cell-wall accumulation of iron: embedding co-catalyst for efficient biomass conversion SO BIOTECHNOLOGY FOR BIOFUELS LA English DT Article DE Ferritin; Iron co-catalyst; Iron accumulation; Transgenic Arabidopsis; Biomass; High-throughput hot-water pretreatment; Saccharification; Sugar release; Perls' Prussian blue staining; X-ray fluorescence microscopy ID RAY-FLUORESCENCE MICROSCOPY; DILUTE-ACID PRETREATMENT; SOYBEAN FERRITIN GENE; HOMEOSTASIS ALTERATION; ARABIDOPSIS MUTANT; LIGNIN COMPOSITION; ESCHERICHIA-COLI; EPITHELIAL-CELLS; BINDING-PROTEIN; CICER-ARIETINUM AB Background: Plant lignocellulosic biomass is an abundant, renewable feedstock for the production of biobased fuels and chemicals. Previously, we showed that iron can act as a co-catalyst to improve the deconstruction of lignocellulosic biomass. However, directly adding iron catalysts into biomass prior to pretreatment is diffusion limited, and increases the cost of biorefinery operations. Recently, we developed a new strategy for expressing iron-storage protein ferritin intracellularly to accumulate iron as a catalyst for the downstream deconstruction of lignocellulosic biomass. In this study, we extend this approach by fusing the heterologous ferritin gene with a signal peptide for secretion into Arabidopsis cell walls (referred to here as FerEX). Results: The transgenic Arabidopsis plants. FerEX. accumulated iron under both normal and iron-fertilized growth conditions; under the latter (iron-fertilized) condition, FerEX transgenic plants showed an increase in plant height and dry weight by 12 and 18 %, respectively, compared with the empty vector control plants. The SDS- and native-PAGE separation of cell-wall protein extracts followed by Western blot analyses confirmed the extracellular expression of ferritin in FerEX plants. Meanwhile, Perls' Prussian blue staining and X-ray fluorescence microscopy (XFM) maps revealed iron depositions in both the secondary and compound middle lamellae cell-wall layers, as well as in some of the corner compound middle lamella in FerEX. Remarkably, their harvested biomasses showed enhanced pretreatability and digestibility, releasing, respectively, 21 % more glucose and 34 % more xylose than the empty vector control plants. These values are significantly higher than those of our recently obtained ferritin intracellularly expressed plants. Conclusions: This study demonstrated that extracellular expression of ferritin in Arabidopsis can produce plants with increased growth and iron accumulation, and reduced thermal and enzymatic recalcitrance. The results are attributed to the intimate colocation of the iron co-catalyst and the cellulose and hemicellulose within the plant cell-wall region, supporting the genetic modification strategy for incorporating conversion catalysts into energy crops prior to harvesting or processing at the biorefinery. C1 [Lin, Chien-Yuan; Donohoe, Bryon S.; Ciesielski, Peter N.; Ding, Shi-You; Himmel, Michael E.; Wei, Hui] Natl Renewable Energy Lab, Biosci Ctr, Golden, CO 80401 USA. [Jakes, Joseph E.] US Forest Serv, Forest Biopolymer Sci & Engn, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Yang, Haibing; McCann, Maureen C.] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. [Gleber, Sophie-Charlotte; Vogt, Stefan] Argonne Natl Lab, Adv Photon Source, Xray Sci Div, Argonne, IL 60439 USA. [Peer, Wendy A.] Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA. [Murphy, Angus S.] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA. [Tucker, Melvin P.] Natl Renewable Energy Lab, Natl Bioenergy Ctr, Golden, CO 80401 USA. [Ding, Shi-You] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. RP Wei, H (reprint author), Natl Renewable Energy Lab, Biosci Ctr, Golden, CO 80401 USA.; Tucker, MP (reprint author), Natl Renewable Energy Lab, Natl Bioenergy Ctr, Golden, CO 80401 USA. EM Melvin.Tucker@nrel.gov; Hui.Wei@nrel.gov FU Center for Direct Catalytic Conversion of Biomass to Biofuels (C3Bio); Energy Frontier Research Center - US Department of Energy, the Office of Science, at the Office of Basic Energy Sciences [DE-SC0000997]; US Department of Energy [DE-AC36-08-GO28308]; US Department of Energy, the Office of Science, at Office of Basic Energy Sciences [W-31-109-Eng-38]; USDA PECASE award FX This work was supported by the Center for Direct Catalytic Conversion of Biomass to Biofuels (C3Bio), an Energy Frontier Research Center funded by the US Department of Energy, the Office of Science, at the Office of Basic Energy Sciences under Award Number DE-SC0000997. The National Renewable Energy Laboratory (NREL) is operated for the US Department of Energy under Contract No. DE-AC36-08-GO28308. The use of Advanced Photon Source facilities was supported by the US Department of Energy, the Office of Science, at Office of Basic Energy Sciences under contract number W-31-109-Eng-38. JEJ acknowledges funding from 2011 USDA PECASE award. NR 65 TC 0 Z9 0 U1 8 U2 8 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1754-6834 J9 BIOTECHNOL BIOFUELS JI Biotechnol. Biofuels PD OCT 21 PY 2016 VL 9 AR 225 DI 10.1186/s13068-016-0639-2 PG 15 WC Biotechnology & Applied Microbiology; Energy & Fuels SC Biotechnology & Applied Microbiology; Energy & Fuels GA DZ8BA UT WOS:000386092200002 PM 27777626 ER PT J AU Jiang, Y Li, CY Takeda, F AF Jiang, Yu Li, Changying Takeda, Fumiomi TI Nondestructive Detection and Quantification of Blueberry Bruising using Near-infrared (NIR) Hyperspectral Reflectance Imaging SO SCIENTIFIC REPORTS LA English DT Article ID APPLES; CLASSIFICATION; FIRMNESS; QUALITY; FRUIT; TIME AB Currently, blueberry bruising is evaluated by either human visual/tactile inspection or firmness measurement instruments. These methods are destructive, time-consuming, and subjective. The goal of this paper was to develop a non-destructive approach for blueberry bruising detection and quantification. Experiments were conducted on 300 samples of southern highbush blueberry (Camellia, Rebel, and Star) and on 1500 samples of northern highbush blueberry (Bluecrop, Jersey, and Liberty) for hyperspectral imaging analysis, firmness measurement, and human evaluation. An algorithm was developed to automatically calculate a bruise ratio index (ratio of bruised to whole fruit area) for bruise quantification. The spectra of bruised and healthy tissues were statistically separated and the separation was independent of cultivars. Support vector machine (SVM) classification of the spectra from the regions of interest (ROIs) achieved over 94%, 92%, and 96% accuracy on the training set, independent testing set, and combined set, respectively. The statistical results showed that the bruise ratio index was equivalent to the measured firmness but better than the predicted firmness in regard to effectiveness of bruise quantification, and the bruise ratio index had a strong correlation with human assessment (R2 = 0.78 - 0.83). Therefore, the proposed approach and the bruise ratio index are effective to non-destructively detect and quantify blueberry bruising. C1 [Jiang, Yu; Li, Changying] Univ Georgia, Coll Engn, Athens, GA 30602 USA. [Takeda, Fumiomi] USDA ARS, Appalachian Fruit Res Stn, 45 Wiltshire Rd, Kearneysville, WV 25430 USA. RP Li, CY (reprint author), Univ Georgia, Coll Engn, Athens, GA 30602 USA. EM cyli@uga.edu FU USDA National Institute of Food and Agriculture Specialty Crop Research Initiative [2014-51181-22383] FX This study is supported by the USDA National Institute of Food and Agriculture Specialty Crop Research Initiative (Award No: 2014-51181-22383). The authors gratefully thank Ms. Renee Holland (University of Georgia Extension), and Mr. David Trinka and Mr. Rolland Groenick (Michigan Blueberry Growers) for their assistance in collecting blueberry samples used in this study, as well as Mr. Jesse Kuzy and Dr. Randy Beaudry's group (Mr. Patrick Abeli, Dr. Nihad Alsmairat, Mr. George Guimares, and Ms. Rosella Briano) for their assistance in conducting the experiments. The authors also especially thank Ms. Megan Lusher (UGA), Dr. Ann Rose and Mr. Jedediah Smith (USDA ARS) for providing an excellent technical assistance. NR 28 TC 0 Z9 0 U1 13 U2 13 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD OCT 21 PY 2016 VL 6 AR 35679 DI 10.1038/srep35679 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ4LX UT WOS:000385831300002 PM 27767050 ER PT J AU Krivan, V Lewis, M Bentz, BJ Bewick, S Lenhart, SM Liebhold, A AF Krivan, Vlastimil Lewis, Mark Bentz, Barbara J. Bewick, Sharon Lenhart, Suzanne M. Liebhold, Andrew TI A dynamical model for bark beetle outbreaks SO JOURNAL OF THEORETICAL BIOLOGY LA English DT Article DE Bistability; Bark beetle; Dendroctonus ponderosae; Dispersal; Filippov solution; Hysteresis; Population dynamics; Stability; SI models ID MOUNTAIN PINE-BEETLE; WESTERN UNITED-STATES; DENDROCTONUS-PONDEROSAE; SPATIOTEMPORAL PATTERNS; NATURAL DISTURBANCES; POPULATION-DYNAMICS; SPATIAL-PATTERNS; RANGE EXPANSION; FOREST; INSECT AB Tree-killing bark beetles are major disturbance agents affecting coniferous forest ecosystems. The role of environmental conditions on driving beetle outbreaks is becoming increasingly important as global climatic change alters environmental factors, such as drought stress, that, in turn, govern tree resistance. Furthermore, dynamics between beetles and trees are highly nonlinear, due to complex aggregation behaviors exhibited by beetles attacking trees. Models have a role to play in helping unravel the effects of variable tree resistance and beetle aggregation on bark beetle outbreaks. In this article we develop a new mathematical model for bark beetle outbreaks using an analogy with epidemiological models. Because the model operates on several distinct time scales, singular perturbation methods are used to simplify the model. The result is a dynamical system that tracks populations of uninfested and infested trees. A limiting case of the model is a discontinuous function of state variables, leading to solutions in the Filippov sense. The model assumes an extensive seed-bank so that tree recruitment is possible even if trees go extinct. Two scenarios are considered for immigration of new beetles. The first is a single tree stand with beetles immigrating from outside while the second considers two forest stands with beetle dispersal between them. For the seed-bank driven recruitment rate, when beetle immigration is low, the forest stand recovers to a beetle-free state. At high beetle immigration rates beetle populations approach an endemic equilibrium state. At intermediate immigration rates, the model predicts bistability as the forest can be in either of the two equilibrium states: a healthy forest, or a forest with an endemic beetle population. The model bistability leads to hysteresis. Interactions between two stands show how a less resistant stand of trees may provide an initial toe-hold for the invasion, which later leads to a regional beetle outbreak in the resistant stand. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Krivan, Vlastimil] Acad Sci Czech Republic, Inst Entomol, Ctr Biol, Branisovska 31, Ceske Budejovice 37005, Czech Republic. [Krivan, Vlastimil] Univ South Bohemia, Fac Sci, Branisovska 1760, Ceske Budejovice 37005, Czech Republic. [Lewis, Mark] Univ Alberta, Dept Math & Stat Sci, Edmonton, AB T6G 2G1, Canada. [Bentz, Barbara J.] USFS Rocky Mt, Res Stn, 860 N 1200 East, Logan, UT 84321 USA. [Bewick, Sharon; Lenhart, Suzanne M.] Natl Inst Math & Biol Synth, 1534 White Ave, Knoxville, TN 37996 USA. [Liebhold, Andrew] USDA Forest Serv, 180 Canfield St, Morgantown, WV 26505 USA. RP Krivan, V (reprint author), Acad Sci Czech Republic, Inst Entomol, Ctr Biol, Branisovska 31, Ceske Budejovice 37005, Czech Republic. EM vlastimil.krivan@gmail.com; mark.lewis@ualberta.ca; bbentz@fs.fed.us; sharon.bewick@gmail.com; lenhart@math.utk.edu; aliebhold@fs.fed.us FU National Science Foundation; U.S. Department of Homeland Security; U.S. Department of Agriculture through NSF Award [EF-0832858]; The University of Tennessee, Knoxville; Institute of Entomology [RVO:60077344]; NSERC Discovery and Accelerator grants; Canada Research Chair; Killam Fellowship; Natural Science and Engineering Research Council of Canada [NET GP 434810-12] FX This work was conducted as a part of the Forest Insect Pests Working Group at the National Institute for Mathematical and Biological Synthesis, sponsored by the National Science Foundation, the U.S. Department of Homeland Security, and the U.S. Department of Agriculture through NSF Award #EF-0832858, with additional support from The University of Tennessee, Knoxville. V. K. acknowledges support provided by the Institute of Entomology (RVO:60077344). M.A.L. gratefully acknowledges support from NSERC Discovery and Accelerator grants, a Canada Research Chair and a Killam Fellowship. This research was also supported by a grant to MAL from the Natural Science and Engineering Research Council of Canada (grant no. NET GP 434810-12) to the TRIA Network, with contributions from Alberta Agriculture and Forestry, Foothills Research Institute, Manitoba Conservation and Water Stewardship, Natural Resources Canada Canadian Forest Service, Northwest Territories Environment and Natural Resources, Ontario Ministry of Natural Resources and Forestry, Saskatchewan Ministry of Environment, West Fraser and Weyerhaeuser. NR 48 TC 0 Z9 0 U1 44 U2 44 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0022-5193 EI 1095-8541 J9 J THEOR BIOL JI J. Theor. Biol. PD OCT 21 PY 2016 VL 407 BP 25 EP 37 DI 10.1016/j.jtbi.2016.07.009 PG 13 WC Biology; Mathematical & Computational Biology SC Life Sciences & Biomedicine - Other Topics; Mathematical & Computational Biology GA DV7JI UT WOS:000383111800003 PM 27396358 ER PT J AU Park, KT ElNaggar, MM Abdellrazeq, GS Bannantine, JP Mack, V Fry, LM Davis, WC AF Park, Kun Taek ElNaggar, Mahmoud M. Abdellrazeq, Gaber S. Bannantine, John P. Mack, Victoria Fry, Lindsay M. Davis, William C. TI Phenotype and Function of CD209(+) Bovine Blood Dendritic Cells, Monocyte-Derived-Dendritic Cells and Monocyte-Derived Macrophages SO PLOS ONE LA English DT Article ID AVIUM SUBSP PARATUBERCULOSIS; RESPIRATORY SYNCYTIAL VIRUS; DC-SIGN; IMMUNE-RESPONSES; IDENTIFICATION; SUBPOPULATIONS; PATHOGENESIS; RECEPTOR; MUTANTS; ANTIGEN AB Phylogenic comparisons of the mononuclear phagocyte system (MPS) of humans and mice demonstrate phenotypic divergence of dendritic cell (DC) subsets that play similar roles in innate and adaptive immunity. Although differing in phenotype, DC can be classified into four groups according to ontogeny and function: conventional DC (cDC1 and cDC2), plasmacytoid DC (pDC), and monocyte derived DC (MoDC). DC of Artiodactyla (pigs and ruminants) can also be sub-classified using this system, allowing direct functional and phenotypic comparison of MoDC and other DC subsets trafficking in blood (bDC). Because of the high volume of blood collections required to study DC, cattle offer the best opportunity to further our understanding of bDC and MoDC function in an outbred large animal species. As reported here, phenotyping DC using a monoclonal antibody (mAb) to CD209 revealed CD209 is expressed on the major myeloid population of DC present in blood and MoDC, providing a phenotypic link between these two subsets. Additionally, the present study demonstrates that CD209 is also expressed on monocyte derived macrophages (MoF). Functional analysis revealed each of these populations can take up and process antigens (Ags), present them to CD4 and CD8 T cells, and elicit a T-cell recall response. Thus, bDC, MoDC, and MoF pulsed with pathogens or candidate vaccine antigens can be used to study factors that modulate DC-driven T-cell priming and differentiation ex vivo. C1 [Park, Kun Taek; ElNaggar, Mahmoud M.; Abdellrazeq, Gaber S.; Mack, Victoria; Fry, Lindsay M.; Davis, William C.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Park, Kun Taek] Seoul Natl Univ, Dept Vet Microbiol, Coll Vet Med, Seoul 151742, South Korea. [ElNaggar, Mahmoud M.; Abdellrazeq, Gaber S.] Univ Alexandria, Fac Vet Med, Dept Microbiol, Alexandria, Egypt. [Bannantine, John P.] ARS, USDA, Natl Anim Dis Ctr, Ames, IA USA. [Fry, Lindsay M.] ARS, USDA, Anim Dis Res Unit, Pullman, WA 99164 USA. RP Davis, WC (reprint author), Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. EM davisw@vetmed.wsu.edu RI Abdellrazeq, Gaber/C-7667-2015 OI Abdellrazeq, Gaber/0000-0002-8677-911X FU Washington State University Monoclonal Antibody Center; Egyptian government; USAID FX This study was supported by the Washington State University Monoclonal Antibody Center (http://vmp.vetmed.wsu.edu/resources/ monoclonal-antibody center). Mahmoud M. ElNaggar was supported in part by a fellowship from the Egyptian government. Gaber S. Abdellrazeq was supported by a fellowship from the USAID. NR 33 TC 1 Z9 1 U1 3 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD OCT 20 PY 2016 VL 11 IS 10 AR e0165247 DI 10.1371/journal.pone.0165247 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ9NZ UT WOS:000386204500140 PM 27764236 ER PT J AU Li, XR Zhang, FM Coates, B Zhang, YH Zhou, XG Cheng, DF AF Li, Xiangrui Zhang, Fangmei Coates, Brad Zhang, Yunhui Zhou, Xuguo Cheng, Dengfa TI Comparative profiling of microRNAs in the winged and wingless English grain aphid, Sitobion avenae (F.) (Homoptera: Aphididae) SO SCIENTIFIC REPORTS LA English DT Article ID SILKWORM BOMBYX-MORI; GENE-EXPRESSION; IMAGINAL DISCS; PEA APHID; DROSOPHILA; RNA; IDENTIFICATION; GROWTH; MORPHS; TRANSCRIPTION AB MicroRNAs (miRNAs) are short single-stranded non-coding RNAs that regulate gene expression, particularly during development. In this study, 345 miRNAs were identified from the English green aphid, Sitobion avenae (F.), of which 168 were conserved and 177 were S. avenae-specific. Quantitative comparison of miRNA expression levels indicated that 16 and 12 miRNAs were significantly up-regulated in winged and wingless S. avenae small RNA libraries, respectively. Differential expression of these miRNAs was confirmed by real-time quantitative RT-PCR validation. The putative transcript targets for these candidate miRNAs were predicted based on sequences from a model species Drosophila melanogaster and four aphid species Acyrthosiphon pisum, Myzus persicae, Toxoptera citricida, and Aphis gosspii. Gene Ontology and KEGG pathway analyses shed light on the potential functions of these miRNAs in the regulation of genes involved in the metabolism, development and wing polyphenism of S. avenae. C1 [Li, Xiangrui; Zhang, Fangmei; Zhang, Yunhui; Cheng, Dengfa] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. [Zhang, Fangmei] Xinyang Agr & Forestry Univ, Xinyang 464000, Henan, Peoples R China. [Coates, Brad] USDA ARS, USDA, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Zhou, Xuguo] Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA. RP Cheng, DF (reprint author), Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China.; Zhou, XG (reprint author), Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA. EM xuguozhou@uky.edu; dfcheng@ippcaas.cn FU National Natural Science Foundation of China [31301659]; National "Twelfth Five-Year" Plan for Science & Technology Support Development Program of China [2012BAD19B04]; China Agriculture Research System [CARS-3]; project of the Kentucky Agricultural Experiment Station [16-08-098] FX The authors are grateful to anonymous reviewers and editor for their constructive criticisms. This research was supported by the National Natural Science Foundation of China (Award number: 31301659), National "Twelfth Five-Year" Plan for Science & Technology Support Development Program of China (Award number: 2012BAD19B04), China Agriculture Research System (Award number: CARS-3). The information reported in this paper (No. 16-08-098) is part of a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 64 TC 0 Z9 1 U1 13 U2 13 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD OCT 20 PY 2016 VL 6 AR 35668 DI 10.1038/srep35668 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ9MU UT WOS:000386200300001 PM 27762301 ER PT J AU de Dios, VR Gessler, A Ferrio, JP Alday, JG Bahn, M del Castillo, J Devidal, S Garcia-Munoz, S Kayler, Z Landais, D Martin-Gomez, P Milcu, A Piel, C Pirhofer-Walzl, K Ravel, O Salekin, S Tissue, DT Tjoelker, MG Voltas, J Roy, J AF Resco de Dios, Victor Gessler, Arthur Pedro Ferrio, Juan Alday, Josu G. Bahn, Michael del Castillo, Jorge Devidal, Sebastien Garcia-Munoz, Sonia Kayler, Zachary Landais, Damien Martin-Gomez, Paula Milcu, Alexandru Piel, Clement Pirhofer-Walzl, Karin Ravel, Olivier Salekin, Serajis Tissue, David T. Tjoelker, Mark G. Voltas, Jordi Roy, Jacques TI Circadian rhythms have significant effects on leaf-to-canopy scale gas exchange under field conditions SO GIGASCIENCE LA English DT Article DE Circadian clock; Ecological memory; Net ecosystem exchange; Scaling; Stomatal conductance models; Photosynthesis; Transpiration ID CARBON-DIOXIDE EXCHANGE; STOMATAL CONDUCTANCE; WATER-USE; PHOTOSYNTHESIS; TRANSPIRATION; GROWTH; PLANTS; ASSIMILATION; ARABIDOPSIS; ATMOSPHERE AB Background: Molecular clocks drive oscillations in leaf photosynthesis, stomatal conductance, and other cell and leaf-level processes over similar to 24 h under controlled laboratory conditions. The influence of such circadian regulation over whole-canopy fluxes remains uncertain; diurnal CO2 and H2O vapor flux dynamics in the field are currently interpreted as resulting almost exclusively from direct physiological responses to variations in light, temperature and other environmental factors. We tested whether circadian regulation would affect plant and canopy gas exchange at the Montpellier European Ecotron. Canopy and leaf-level fluxes were constantly monitored under field-like environmental conditions, and under constant environmental conditions (no variation in temperature, radiation, or other environmental cues). Results: We show direct experimental evidence at canopy scales of the circadian regulation of daytime gas exchange: 20-79 % of the daily variation range in CO2 and H2O fluxes occurred under circadian entrainment in canopies of an annual herb (bean) and of a perennial shrub (cotton). We also observed that considering circadian regulation improved performance by 8-17 % in commonly used stomatal conductance models. Conclusions: Our results show that circadian controls affect diurnal CO2 and H2O flux patterns in entire canopies in field-like conditions, and its consideration significantly improves model performance. Circadian controls act as a 'memory' of the past conditions experienced by the plant, which synchronizes metabolism across entire plant canopies. C1 [Resco de Dios, Victor; Pedro Ferrio, Juan; Alday, Josu G.; del Castillo, Jorge; Martin-Gomez, Paula; Voltas, Jordi] Univ Lleida, Agrotecn Ctr, Dept Crop & Forest Sci, Lleida 25198, Spain. [Gessler, Arthur] Swiss Fed Inst Forest Snow & Landscape Res, Long Term Forest Ecosyst Res, CH-8903 Birmensdorf, Switzerland. [Gessler, Arthur; Kayler, Zachary; Pirhofer-Walzl, Karin] Leibniz Ctr Agr Landscape Res, Inst Landscape Biogeochem, D-15374 Muncheberg, Germany. [Pedro Ferrio, Juan] Univ Concepcion, Fac Ciencias Nat & Oceanog, Dept Bot, Casilla 160-C, Concepcion, Chile. [Alday, Josu G.] Univ Liverpool, Sch Environm Sci, Liverpool L69 3GP, Merseyside, England. [Bahn, Michael] Univ Innsbruck, Inst Ecol, A-6020 Innsbruck, Austria. [Devidal, Sebastien; Landais, Damien; Milcu, Alexandru; Piel, Clement; Ravel, Olivier; Roy, Jacques] CNRS, Ecotron Europeen Montpellier, UPS 3248, Campus Baillarguet, F-34980 Montferrier Sur Lez, France. [Garcia-Munoz, Sonia] Inst Madrileno Invest & Desarrollo Rural Agr & Al, Madrid 28800, Spain. [Kayler, Zachary] US Forest Serv, USDA, Northern Res Stn, Lawrence Livermore Natl Lab, Livermore, CA 94550 USA. [Milcu, Alexandru] Univ Montpellier, Univ Paul Valery, CNRS, Ctr Ecol Fonct & Evolut,Ecole Prat Hautes Etud,UM, F-34293 Montpellier 5, France. [Pirhofer-Walzl, Karin] Free Univ Berlin, Inst Biol Plant Ecol, D-14195 Berlin, Germany. [Pirhofer-Walzl, Karin] Berlin Brandenburg Inst Adv Biodivers Res, D-14195 Berlin, Germany. [Salekin, Serajis] Univ Lleida, Erasmus Mundus Master Mediterranean Forestry & Na, Lleida 25198, Spain. [Salekin, Serajis] Univ Canterbury, Coll Engn, Sch Forestry, Christchurch 8140, New Zealand. [Tissue, David T.; Tjoelker, Mark G.] Univ Western Sydney, Hawkesbury Inst Environm, Richmond, NSW 2753, Australia. RP de Dios, VR (reprint author), Univ Lleida, Agrotecn Ctr, Dept Crop & Forest Sci, Lleida 25198, Spain. EM v.rescodedios@gmail.com RI Gessler, Arthur/C-7121-2008; Ferrio, Juan Pedro/A-5748-2008; Tjoelker, Mark/M-2413-2016; Alday, Josu G./F-6175-2010; Resco de Dios, Victor/G-5555-2014 OI Gessler, Arthur/0000-0002-1910-9589; Ferrio, Juan Pedro/0000-0001-5904-7821; Tjoelker, Mark/0000-0003-4607-5238; Alday, Josu G./0000-0001-7510-8655; Resco de Dios, Victor/0000-0002-5721-1656 FU Ramon y Cajal fellowships [RYC-2012-10970, RYC-2008-02050]; Leibniz Centre for Agricultural Landscape Research; Western Sydney University's Hawkesbury Institute for the Environment; Spanish Government [AGL2015-69151-R] FX This study benefited from the CNRS human and technical resources allocated to the Research Infrastructure Ecotrons, as well as from the state allocation 'Investissement d'Avenir' ANR-11-INBS-0001; ExpeER Transnational Access program; Ramon y Cajal fellowships (RYC-2012-10970 to VRD and RYC-2008-02050 to JPF); the Erasmus Mundus Master Course Mediterranean Forestry and Natural Resources Management (MEDfOR); and internal grants from the Leibniz Centre for Agricultural Landscape Research to AG, and from the Western Sydney University's Hawkesbury Institute for the Environment and the Spanish Government (AGL2015-69151-R) to VRD. NR 56 TC 0 Z9 0 U1 21 U2 21 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 2047-217X J9 GIGASCIENCE JI GigaScience PD OCT 20 PY 2016 VL 5 AR 43 DI 10.1186/s13742-016-0149-y PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ1ZH UT WOS:000385640200001 ER PT J AU Xu, GL Singh, SK Reddy, VR Barnaby, JY Sicher, RC Li, T AF Xu, Guangli Singh, Shardendu K. Reddy, Vangimalla R. Barnaby, Jinyoung Y. Sicher, Richard C. Li, Tian TI Soybean grown under elevated CO2 benefits more under low temperature than high temperature stress: Varying response of photosynthetic limitations, leaf metabolites, growth, and seed yield SO JOURNAL OF PLANT PHYSIOLOGY LA English DT Article DE Carbohydrate; Organic acids; Over compensation; Photosynthetic limitation; Quantum yield; Starch ID CARBON-DIOXIDE CONCENTRATION; MESOPHYLL CONDUCTANCE; ELECTRON-TRANSPORT; CHLOROPHYLL FLUORESCENCE; OXIDATIVE STRESS; GAS-EXCHANGE; PLANT-GROWTH; IN-VIVO; ENRICHMENT; RUBISCO AB To evaluate the combined effect of temperature and CO2 on photosynthetic processes, leaf metabolites and growth, soybean was grown under a controlled environment at low (22/18 degrees C, LT), optimum (28/24 degrees C, OT) and high (36/32 degrees C HT) temperatures under ambient (400 mu mol mol(-1); aCO(2)) or elevated (800 mu mol mol(-1); eCO(2)) CO2 concentrations during the reproductive stage. In general, the rate of photosynthesis (A), stomatal (g(s)) and mesophyll (g(m)) conductance, quantum yield of photosystem II, rates of maximum carboxylation (V-Cmax), and electron transport (J) increased with temperature across CO2 levels. However, compared with OT, the percentage increases in these parameters at HT were lower than the observed decline at LT. The photosynthetic limitation at LT and OT was primarily caused by photo-biochemical processes (49-58%, L-b) followed by stomatal (27-32%, L-s) and mesophyll (15-19%, L-m) limitations. However, at HT, it was primarily caused by L-s (41%) followed by L-b (33%) and L-m (26%). The dominance of L-b at LT and OT was associated with the accumulation of non-structural carbohydrates (e.g., starch) and several organic acids, whereas this accumulation did not occur at HT, indicating increased metabolic activities. Compared with OT, biomass and seed yield declined more at HT than at LT. The eCO(2) treatment compensated for the temperature-stress effects on biomass but only partially compensated for the effects on seed yield, especially at HT. Photosynthetic downregulation at eCO(2) was possibly due to the accumulation of non-structural carbohydrates and the decrease in g(s) and A(std) (standard A measured at 400 mu mol mol(-1) sub-stomatal CO2 concentration), as well as the lack of CO2 effect on g(m), V-Cmax,V- and J, and photosynthetic limitation. Thus, the photosynthetic limitation was temperature-dependent and was primarily influenced by the alteration in photo-biochemical processes and metabolic activities. Despite the inconsistent response of photosynthesis (or biomass accumulation) and seed yield, eCO(2) tended to fully or partially compensate for the adverse effect of the respective LT and HT stresses under well-watered and sufficient nutrient conditions. (C) 2016 Elsevier GmbH. All rights reserved. C1 [Xu, Guangli; Singh, Shardendu K.; Reddy, Vangimalla R.; Barnaby, Jinyoung Y.; Sicher, Richard C.] ARS, Crop Syst & Global Change Lab, USDA, Bldg 001,Rm 342,BARC West,10300 Baltimore Ave, Beltsville, MD 20705 USA. [Xu, Guangli; Li, Tian] Sichuan Agr Univ, Coll Agron, Chengdu, Peoples R China. [Singh, Shardendu K.] Univ Maryland, Wye Res & Educ Ctr, College Pk, MD 20742 USA. RP Singh, SK (reprint author), ARS, Crop Syst & Global Change Lab, USDA, Bldg 001,Rm 342,BARC West,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM shardendu.singh@ars.usda.gov NR 61 TC 0 Z9 0 U1 30 U2 30 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 0176-1617 EI 1618-1328 J9 J PLANT PHYSIOL JI J. Plant Physiol. PD OCT 20 PY 2016 VL 205 BP 20 EP 32 DI 10.1016/j.jplph.2016.08.003 PG 13 WC Plant Sciences SC Plant Sciences GA DZ4VL UT WOS:000385858600003 PM 27589223 ER PT J AU Yangcheng, H Belamkar, V Cannon, SB Jane, JL AF Yangcheng, Hanyu Belamkar, Vikas Cannon, Steven B. Jane, Jay-lin TI Characterization and development mechanism of Apios americana tuber starch SO CARBOHYDRATE POLYMERS LA English DT Article DE Apios; Mother tuber; Child tuber; Starch; Double thermal-transitiona ID PASTING PROPERTIES; MAIZE STARCH; GELATINIZATION; MEDIKUS; GROWTH; BARLEY; ACIDS AB Apios americana is a wild legume-bearing plant with edible tubers. Domestication of Apios is in progress because of the superior nutritional value and health benefits of the tuber. Objectives of this study were to: (1) characterize physicochemical properties of the Apios tuber starch; and (2) understand differences in starch structures and properties between the mother (seed) and child (progeny) tubers and the mechanism of starch development. Granules of the Apios tuber starch displayed ellipsoidal, rod, or kidney shape with diameter ranges of 1-30 mu m. The mother tuber starches displayed greater percentage crystallinity, larger gelatinization enthalpy-changes, longer branch-chain lengths of amylopectin, and lower pasting viscosity than their counterpart child tuber starches. The mother tuber starch of Apios 2127 displayed distinct two peaks of gelatinization, which were attributed to starch granules located at different regions of the tuber having different structures and properties. The mother tuber displayed more active starch biosynthesis in the periphery than in the center of the tuber. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Yangcheng, Hanyu; Jane, Jay-lin] Iowa State Univ, Dept Food Sci & Human Nutr, Ames, IA 50011 USA. [Belamkar, Vikas] Iowa State Univ, Interdept Genet, Ames, IA 50011 USA. [Cannon, Steven B.] Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Cannon, Steven B.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. RP Jane, JL (reprint author), Iowa State Univ, Dept Food Sci & Human Nutr, Ames, IA 50011 USA. EM jjane@iastate.edu NR 29 TC 1 Z9 1 U1 6 U2 6 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD OCT 20 PY 2016 VL 151 BP 198 EP 205 DI 10.1016/j.carbpol.2016.05.062 PG 8 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA DS7DV UT WOS:000380943900022 PM 27474558 ER PT J AU Liu, YQ Wisniewski, M Kennedy, JF Jiang, YS Tang, JM Liu, J AF Liu, Yiqing Wisniewski, Michael Kennedy, John F. Jiang, Yusong Tang, Jianmin Liu, Jia TI Chitosan and oligochitosan enhance ginger (Zingiber officinale Roscoe) resistance to rhizome rot caused by Fusarium oxysporum in storage SO CARBOHYDRATE POLYMERS LA English DT Article DE Chitosan; Oligochitosan; Ginger rhizome; Defense response; Fusarium oxysporum ID POSTHARVEST DISEASES; FRUIT; ANTHRACNOSE; DEFENSE; QUALITY; DERIVATIVES; ANTIFUNGAL; PATHOGENS; RESPONSES; COATINGS AB The ability of chitosan and oligochitosan to enhance ginger (Zingiber officinale) resistance to rhizome rot caused by Fusarium oxysporum in storage was investigated. Both chitosan and oligochitosan at 1 and 5 g/L significantly inhibited rhizome rot, with the best control at 5 g/L. Chitosan and oligochitosan applied at 5 g/L also reduced weight loss, measured as a decrease in fresh weight, but did not affect soluble solids content or titratable acidity of rhizomes. The two compounds applied at 5 g/L induced beta-1,3-glucanase and phenylalanine ammonia-lyase enzyme activity and the transcript levels of their coding genes, as well as the total phenolic compounds in rhizome tissues. Therefore, the ability of chitosan and oligochitosan to reduce rot in stored rhizomes may be associated with their ability to induce defense responses in ginger. These results have practical implications for the application of chitosan and oligochitosan to harvested ginger rhizomes to reduce postharvest losses. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Liu, Yiqing; Jiang, Yusong; Tang, Jianmin; Liu, Jia] Chongqing Univ Arts & Sci, Coll Forestry & Life Sci, Inst Special Plants, Yongchuan 402160, Peoples R China. [Liu, Yiqing; Jiang, Yusong; Tang, Jianmin; Liu, Jia] Collabrat Innovatat Ctr Special Plant Ind Chongqi, Chongqing Engn Res Ctr Special Plant Seedlings, Garden & Flower Engn Res Ctr Chongqing Coll, Yongchuan 402160, Peoples R China. [Wisniewski, Michael] USDA ARS, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. [Kennedy, John F.] Chembiotech Labs, Kyrewood House, Tenbury Wells WR15 8SG, Worcs, England. RP Liu, J (reprint author), Chongqing Univ Arts & Sci, Coll Forestry & Life Sci, Inst Special Plants, Yongchuan 402160, Peoples R China. EM jialiu1983@163.com FU Scientific Research Fund of the Chongqing Education Commission [KJZH14216]; Scientific Research Fund of the Chongqing Science & Technology Commission [cstc2013jcyjA80035]; National Natural Science Foundation of China [31501273]; Chongqing Key Discipline Construction of Forestry FX This work was supported by the Scientific Research Fund of the Chongqing Education Commission (KJZH14216), the Scientific Research Fund of the Chongqing Science & Technology Commission (cstc2013jcyjA80035), National Natural Science Foundation of China (31501273), and the Chongqing Key Discipline Construction of Forestry. NR 39 TC 0 Z9 0 U1 20 U2 23 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD OCT 20 PY 2016 VL 151 BP 474 EP 479 DI 10.1016/j.carbpol.2016.05.103 PG 6 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA DS7DV UT WOS:000380943900055 PM 27474591 ER PT J AU Xie, JL Hse, CY De Hoop, CF Hu, TX Qi, JQ Shupe, TF AF Xie, Jiulong Hse, Chung-Yun De Hoop, Cornelis F. Hu, Tingxing Qi, Jinqiu Shupe, Todd F. TI Isolation and characterization of cellulose nanofibers from bamboo using microwave liquefaction combined with chemical treatment and ultrasonication SO CARBOHYDRATE POLYMERS LA English DT Article DE Cellulose nanofibers; Microwave liquefaction; Bamboo; Ultrasonic nanofibrillation ID MECHANICAL-PROPERTIES; ENZYMATIC-HYDROLYSIS; REINFORCING AGENT; WHEAT-STRAW; BANANA PEEL; NANOCRYSTALS; COMPOSITES; WOOD; EXTRACTION; COMPONENTS AB Cellulose nanofibers were successfully isolated from bamboo using microwave liquefaction combined with chemical treatment and ultrasonic nanofibrillation processes. The microwave liquefaction could eliminate almost all the lignin in bamboo, resulting in high cellulose content residues within 7 min, and the cellulose enriched residues could be readily purified by subsequent chemical treatments with lower chemical charging and quickly. The results of wet chemistry analyses, SEM images, and FTIR and X-ray spectra indicated the combination of microwave liquefaction and chemical treatment was significantly efficient in removing non-cellulosic compounds. Ultrasonication was used to separate the nanofibrils from the purified residues to extract nanofibers. The TEM images confirmed the presence of elementary fibrils, nano-sized fibril bundles, and aggregated fibril bundles. As evidenced by the TGA analysis, cellulose nanofibers isolated by this novel technique had high thermal stability indicating that the isolated nanofibers could possibly be applied as reinforcing elements in biomaterials. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Xie, Jiulong; Hu, Tingxing; Qi, Jinqiu] Sichuan Agr Univ, Coll Forestry, Chengdu 611130, Sichuan, Peoples R China. [Hse, Chung-Yun] US Forest Serv, Southern Res Stn, USDA, Pineville, LA 71360 USA. [Xie, Jiulong; De Hoop, Cornelis F.; Shupe, Todd F.] Louisiana State Univ, Ctr Agr, Sch Renewable Nat Resource, Baton Rouge, LA 70803 USA. RP Qi, JQ (reprint author), Sichuan Agr Univ, Coll Forestry, Chengdu 611130, Sichuan, Peoples R China. EM qijinqiu2005@aliyun.com NR 57 TC 2 Z9 2 U1 42 U2 51 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD OCT 20 PY 2016 VL 151 BP 725 EP 734 DI 10.1016/j.carbpol.2016.06.011 PG 10 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA DS7DV UT WOS:000380943900083 PM 27474619 ER PT J AU Bilbao-Sainz, C Chiou, BS Valenzuela-Medina, D Imam, SH Vega-Galvez, A Orts, WJ AF Bilbao-Sainz, Cristina Chiou, Bor-Sen Valenzuela-Medina, Diana Imam, Syed H. Vega-Galvez, Antonio Orts, William J. TI Biopolymer films to control fusarium dry rot and their application to preserve potato tubers SO JOURNAL OF APPLIED POLYMER SCIENCE LA English DT Article DE chitosan; fludioxonil; Fusarium solani; potatoes; sodium alginate ID EDIBLE CHITOSAN FILMS; DIFFERENT MOLECULAR-WEIGHTS; ANTIMICROBIAL ACTIVITY; LISTERIA-MONOCYTOGENES; FOODBORNE PATHOGENS; PHYSICAL-PROPERTIES; COMPOSITE FILMS; ESSENTIAL OIL; NANOPARTICLES; NATAMYCIN AB Films were cast using sodium alginate (NaAlg), high molecular weight (HMW) chitosan, and low molecular weight (LMW) chitosan as film forming biopolymers. Fludioxonil (Fl) at 1% concentration was used as fungicide. Thermal stability, mechanical, and water sorption properties of the films were examined. The effects of films on the Fusarium solani colony radial growth were evaluated in vitro and in potato tubers. Results showed that chitosan films were more thermally stable and less hydrophilic than alginate films. Addition of fluodioxonil to the films significantly reduced the film strength and increased the elongation at break as well as the film stiffness. In vitro studies showed that when fludioxonil was added to the formulation, NaAlg and Chitosan-LMW films had significantly higher antifungal activity (Fungistatic index = 56%) than Chitosan-HMW films (Fungistatic index = 50%). In vivo studies showed that Chitosan-LMW-1% Fl films delay the mycelial growth of F. solani in tubers kept at 25 degrees C for 2 weeks. (C) 2016 Wiley Periodicals, Inc. C1 [Bilbao-Sainz, Cristina; Chiou, Bor-Sen; Valenzuela-Medina, Diana; Imam, Syed H.; Orts, William J.] ARS, Bioprod Chem & Engn, USDA, WRRC, Albany, CA 94710 USA. [Vega-Galvez, Antonio] Univ La Serena, Dept Food Engn, Av Raul Bitran 1305, La Serena, Chile. RP Bilbao-Sainz, C (reprint author), ARS, Bioprod Chem & Engn, USDA, WRRC, Albany, CA 94710 USA. EM cristina.bilbao@ars.usda.gov RI Vega-Galvez, Antonio /F-1089-2015 NR 36 TC 0 Z9 0 U1 12 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8995 EI 1097-4628 J9 J APPL POLYM SCI JI J. Appl. Polym. Sci. PD OCT 20 PY 2016 VL 133 IS 40 AR 44017 DI 10.1002/app.44017 PG 6 WC Polymer Science SC Polymer Science GA DS4AZ UT WOS:000380725100004 ER PT J AU Rasmussen-Ivey, CR Hossain, MJ Odom, SE Terhune, JS Hemstreet, WG Shoemaker, CA Zhang, DH Xu, DH Griffin, MJ Liu, YJ Figueras, MJ Santos, SR Newton, JC Liles, MR AF Rasmussen-Ivey, Cody R. Hossain, Mohammad J. Odom, Sara E. Terhune, Jeffery S. Hemstreet, William G. Shoemaker, Craig A. Zhang, Dunhua Xu, De-Hai Griffin, Matt J. Liu, Yong-Jie Figueras, Maria J. Santos, Scott R. Newton, Joseph C. Liles, Mark R. TI Classification of a Hypervirulent Aeromonas hydrophila Pathotype Responsible for Epidemic Outbreaks in Warm-Water Fishes SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE Aeromonas hydrophila; pathogenesis; comparative genomics; emerging disease; bacteria; catfish; carp ID VI SECRETION SYSTEM; MOLECULAR CHARACTERIZATION; PHYLOGENETIC ANALYSIS; BACTERIAL GENOMES; DISEASE OUTBREAKS; SP-NOV.; SEQUENCE; AQUACULTURE; ANNOTATION; COMPONENTS AB Lineages of hypervirulent Aeromonas hydrophila (vAh) are the cause of persistent outbreaks of motile Aeromonas septicemia in warm water fishes worldwide. Over the last decade, this virulent lineage of A. hydrophila has resulted in annual losses of millions of tons of farmed carp and catfish in the People's Republic of China and the United States (US). Multiple lines of evidence indicate US catfish and Asian carp isolates of A. hydrophila affiliated with sequence type 251 (ST251) share a recent common ancestor. To address the genomic context for the putative intercontinental transfer and subsequent geographic spread of this pathogen, we conducted a core genome phylogenetic analysis on 61 Aeromonas spp. genomes, of which 40 were affiliated with A. hydrophila, with 26 identified as epidemic strains. Phylogenetic analyses indicate all ST251 strains form a coherent lineage affiliated with A. hydrophila. Within this lineage, conserved genetic loci unique to A. hydrophila were identified, with some genes present in consistently higher copy numbers than in non-epidemic A. hydrophila isolates. In addition, results from analyses of representative ST251 isolates support the conclusion that multiple lineages are present within US vAh isolated from Mississippi, whereas vAh isolated from Alabama appear clonal. This is the first report of genomic heterogeneity within US vAh isolates, with some Mississippi isolates showing closer affiliation with the Asian grass carp isolate ZC1 than other vAh isolated in the US. To evaluate the biological significance of the identified heterogeneity, comparative disease challenges were conducted with representatives of different vAh genotypes. These studies revealed that isolate ZC1 yielded significantly lower mortality in channel catfish, relative to Alabama and Mississippi vAh isolates. Like other Asian vAh isolates, the ZC1 lineage contains all core genes for a complete type VI secretion system (T6SS). In contrast, more virulent US isolates retain only remnants of the T6SS (clpB, hcp, vgrG, and vasH) which may have functional implications. Collectively, these results characterize a hypervirulent A. hydrophila pathotype that affects farmed fish on multiple continents. C1 [Rasmussen-Ivey, Cody R.; Hossain, Mohammad J.; Odom, Sara E.; Santos, Scott R.; Liles, Mark R.] Auburn Univ, Dept Biol Sci, Auburn, AL 36849 USA. [Terhune, Jeffery S.] Sch Fisheries Aquaculture & Aquat Sci, Auburn, AL USA. [Hemstreet, William G.] Alabama Fish Farming Ctr, Greensboro, AL USA. [Shoemaker, Craig A.; Zhang, Dunhua; Xu, De-Hai] ARS, Aquat Anim Hlth Res Unit, USDA, Auburn, AL USA. [Griffin, Matt J.] Mississippi State Univ, Coll Vet Med, Thad Cochran Natl Warmwater Aquaculture Ctr, Stoneville, MS USA. [Liu, Yong-Jie] Nanjing Agr Univ, Coll Vet Med, Nanjing, Jiangsu, Peoples R China. [Figueras, Maria J.] Univ Rovira & Virgili, Dept Ciencias Med Basicas, Fac Med & Ciencias Salud, IISPV, Reus, Spain. [Newton, Joseph C.] Auburn Univ, Dept Pathobiol, Auburn, AL 36849 USA. RP Liles, MR (reprint author), Auburn Univ, Dept Biol Sci, Auburn, AL 36849 USA.; Newton, JC (reprint author), Auburn Univ, Dept Pathobiol, Auburn, AL 36849 USA. EM newtojc@aubum.edu; lilesma@auburn.edu RI Santos, Scott/A-7472-2009 FU United States Department of Agriculture's Agriculture and Food Research Initiative [2013-67015-21313, MIS-371530]; USDA-ARS [6010-32000-026-00D]; Catfish Health Initiative; Aquavalens from the European Union Seventh Framework Program [311816]; Spanish Ministry of Science and Innovation [AGL2011-30461-C02-02, JPIW2013-095-CO3] FX This work was supported by a grant from the United States Department of Agriculture's Agriculture and Food Research Initiative (Projects #2013-67015-21313 and #MIS-371530) and the USDA-ARS (CRIS Project No. 6010-32000-026-00D and the Catfish Health Initiative). MF thanks the support by the projects: Aquavalens from the European Union Seventh Framework Program (FP7/2007-2013) under Grant agreement No.: 311816, and by projects from Spanish Ministry of Science and Innovation: AGL2011-30461-C02-02 and JPIW2013-095-CO3. NR 69 TC 1 Z9 1 U1 2 U2 2 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD OCT 18 PY 2016 VL 7 AR 1615 DI 10.3389/fmicb.2016.01615 PG 16 WC Microbiology SC Microbiology GA ED0JS UT WOS:000388529400001 PM 27803692 ER PT J AU Zhang, XH Yan, ZQ Li, XJ Lin, WC Dai, ZK Yan, YM Lu, PP Chen, WG Zhang, HM Chen, F Ma, JY Xie, QM AF Zhang, Xinheng Yan, Zhuanqiang Li, Xinjian Lin, Wencheng Dai, Zhenkai Yan, Yiming Lu, Piaopiao Chen, Weiguo Zhang, Huanmin Chen, Feng Ma, Jingyun Xie, Qingmei TI GADD45 beta, an anti-tumor gene, inhibits avian leukosis virus subgroup J replication in chickens SO ONCOTARGET LA English DT Article DE ALV-J; RNA-Seq; differentially expressed genes; GADD45 beta; viral replication ID RNA-SEQ; MYELOID LEUKOSIS; HPRS-103 STRAIN; MAREKS-DISEASE; LAYER FLOCKS; EXPRESSION; INTEGRATION; INFECTION; IDENTIFICATION; APOPTOSIS AB Avian leukosis virus subgroup J (ALV-J) is a retroviruses that induces neoplasia, hepatomegaly, immunosuppression and poor performance in chickens. The tumorigenic and pathogenic mechanisms of ALV-J remain a hot topic. To explore anti-tumor genes that promote resistance to ALV-J infection in chickens, we bred ALV-J resistant and susceptible chickens (F3 generation). RNA-sequencing (RNA-Seq) of liver tissue from the ALV-J resistant and susceptible chickens identified 216 differentially expressed genes; 88 of those genes were up-regulated in the ALV-J resistant chickens (compared to the susceptible ones). We screened for significantly up-regulated genes (P < 0.01) of interest in the ALV-J resistant chickens, based on their involvement in biological signaling pathways. Functional analyses showed that overexpression of GADD45 beta inhibited ALV-J replication. GADD45 beta could enhance defense against ALV-J infection and may be used as a molecular marker to identify ALV-J infections. C1 [Zhang, Xinheng; Yan, Zhuanqiang; Li, Xinjian; Lin, Wencheng; Dai, Zhenkai; Yan, Yiming; Lu, Piaopiao; Chen, Weiguo; Chen, Feng; Ma, Jingyun; Xie, Qingmei] South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China. [Zhang, Xinheng; Yan, Zhuanqiang; Li, Xinjian; Lin, Wencheng; Dai, Zhenkai; Yan, Yiming; Lu, Piaopiao; Chen, Weiguo; Chen, Feng; Ma, Jingyun; Xie, Qingmei] Minist Agr, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China. [Zhang, Xinheng; Yan, Zhuanqiang; Li, Xinjian; Lin, Wencheng; Dai, Zhenkai; Yan, Yiming; Lu, Piaopiao; Chen, Weiguo; Chen, Feng; Ma, Jingyun; Xie, Qingmei] Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangzhou 510642, Guangdong, Peoples R China. [Zhang, Xinheng; Li, Xinjian; Lin, Wencheng; Dai, Zhenkai; Yan, Yiming; Lu, Piaopiao; Chen, Weiguo; Chen, Feng; Ma, Jingyun; Xie, Qingmei] Key Lab Anim Hlth Aquaculture & Environm Control, Guangzhou 510642, Guangdong, Peoples R China. [Lin, Wencheng; Chen, Weiguo; Chen, Feng; Xie, Qingmei] South China Collaborat Innovat Ctr Poultry Dis Co, Guangzhou 510642, Guangdong, Peoples R China. [Zhang, Huanmin] ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. RP Xie, QM (reprint author), South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China.; Xie, QM (reprint author), Minist Agr, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China.; Xie, QM (reprint author), Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangzhou 510642, Guangdong, Peoples R China.; Xie, QM (reprint author), Key Lab Anim Hlth Aquaculture & Environm Control, Guangzhou 510642, Guangdong, Peoples R China.; Xie, QM (reprint author), South China Collaborat Innovat Ctr Poultry Dis Co, Guangzhou 510642, Guangdong, Peoples R China. EM qmx@scau.edu.cn FU Natural Science Foundation of Guangdong Province [S2013030013313]; National Natural Science Foundation of China [31472217]; Guangdong Province Science and Technology Plan Project [2012B020306002, 2012B091100078] FX This study was supported by the Natural Science Foundation of Guangdong Province (Grant No. S2013030013313), National Natural Science Foundation of China (Grant No. 31472217) and Guangdong Province Science and Technology Plan Project (Grant Nos. 2012B020306002, 2012B091100078). NR 44 TC 0 Z9 0 U1 2 U2 2 PU IMPACT JOURNALS LLC PI ALBANY PA 6211 TIPTON HOUSE, STE 6, ALBANY, NY 12203 USA SN 1949-2553 J9 ONCOTARGET JI Oncotarget PD OCT 18 PY 2016 VL 7 IS 42 BP 68883 EP 68893 DI 10.18632/oncotarget.12027 PG 11 WC Oncology; Cell Biology SC Oncology; Cell Biology GA EB5UT UT WOS:000387446800091 PM 27655697 ER PT J AU Sezen, UU Barney, JN Atwater, DZ Pederson, GA Pederson, JF Chandler, JM Cox, TS Cox, S Dotray, P Kopec, D Smith, SE Schroeder, J Wright, SD Jiao, YN Kong, WQ Goff, V Auckland, S Rainville, LK Pierce, GJ Lemke, C Compton, R Phillips, C Kerr, A Mettler, M Paterson, AH AF Sezen, U. Uzay Barney, Jacob N. Atwater, Daniel Z. Pederson, Gary A. Pederson, Jeffrey F. Chandler, J. Mike Cox, T. Stan Cox, Sheila Dotray, Peter Kopec, David Smith, Steven E. Schroeder, Jill Wright, Steven D. Jiao, Yuannian Kong, Wenqian Goff, Valorie Auckland, Susan Rainville, Lisa K. Pierce, Gary J. Lemke, Cornelia Compton, Rosana Phillips, Christine Kerr, Alexandra Mettler, Matthew Paterson, Andrew H. TI Multi-Phase US Spread and Habitat Switching of a Post-Columbian Invasive, Sorghum halepense SO PLOS ONE LA English DT Article ID POPULATION-STRUCTURE; GENETIC-VARIATION; ADAPTIVE EVOLUTION; JOHNSON GRASS; L PERS; PLANT; RANGE; PROGRAM; GENOME AB Johnsongrass (Sorghum halepense) is a striking example of a post-Columbian founder event. This natural experiment within ecological time-scales provides a unique opportunity for understanding patterns of continent-wide genetic diversity following range expansion. Microsatellite markers were used for population genetic analyses including leaf-optimized Neighbor-Joining tree, pairwise FST, mismatch analysis, principle coordinate analysis, Tajima's D, Fu's F and Bayesian clusterings of population structure. Evidence indicates two geographically distant introductions of divergent genotypes, which spread across much of the US in <200 years. Based on geophylogeny, gene flow patterns can be inferred to have involved five phases. Centers of genetic diversity have shifted from two introduction sites separated by similar to 2000 miles toward the middle of the range, consistent with admixture between genotypes from the respective introductions. Genotyping provides evidence for a 'habitat switch' from agricultural to non-agricultural systems and may contribute to both Johnsongrass ubiquity and aggressiveness. Despite lower and more structured diversity at the invasion front, Johnsongrass continues to advance northward into cooler and drier habitats. Association genetic approaches may permit identification of alleles contributing to the habitat switch or other traits important to weed/invasive management and/or crop improvement. C1 [Sezen, U. Uzay; Kong, Wenqian; Goff, Valorie; Auckland, Susan; Rainville, Lisa K.; Pierce, Gary J.; Lemke, Cornelia; Compton, Rosana; Phillips, Christine; Kerr, Alexandra; Mettler, Matthew; Paterson, Andrew H.] Plant Genome Mapping Lab, Athens, GA 30602 USA. [Barney, Jacob N.; Atwater, Daniel Z.] Virginia Tech, Blacksburg, VA USA. [Pederson, Gary A.] ARS, USDA, PGRCU, Griffin, GA USA. [Pederson, Jeffrey F.] ARS, USDA, Lincoln, NE USA. [Pederson, Jeffrey F.] Univ Nebraska Lincoln, Lincoln, NE USA. [Chandler, J. Mike] Texas A&M Univ, College Stn, TX USA. [Cox, T. Stan; Cox, Sheila] Land Inst, Salina, KS USA. [Dotray, Peter] Texas Tech Univ, Lubbock, TX 79409 USA. [Kopec, David; Smith, Steven E.] Univ Arizona, Tucson, AZ USA. [Schroeder, Jill] New Mexico State Univ, Las Cruces, NM 88003 USA. [Wright, Steven D.] Univ Calif Davis, Davis, CA 95616 USA. [Jiao, Yuannian] Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China. RP Paterson, AH (reprint author), Plant Genome Mapping Lab, Athens, GA 30602 USA. EM paterson@uga.edu OI Barney, Jacob/0000-0003-2949-5003 FU United States Department of Agriculture Biotechnology Risk Assessment Program [08-39211-19568, 2012-33522-19790]; United States Department of Agriculture Controlling Weedy and Invasive Plants program [2013-67013-21306] FX Funding was provided by the United States Department of Agriculture Biotechnology Risk Assessment Program (08-39211-19568 to AHP, 2012-33522-19790 to AHP and TSC), and the United States Department of Agriculture Controlling Weedy and Invasive Plants program (2013-67013-21306 to JNB and AHP). NR 46 TC 0 Z9 0 U1 0 U2 0 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD OCT 18 PY 2016 VL 11 IS 10 AR e0164584 DI 10.1371/journal.pone.0164584 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ9NV UT WOS:000386203800023 PM 27755565 ER PT J AU Li, YQ Liu, C Cui, Y Walse, SS Olver, R Zilberman, D Mitch, WA AF Li, Yuanqing Liu, Chong Cui, Yi Walse, Spencer S. Olver, Ryan Zilberman, David Mitch, William A. TI Development of an Activated Carbon-Based Electrode for the Capture and Rapid Electrolytic Reductive Debromination of Methyl Bromide from Postharvest Fumigations SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID DISINFECTION BY-PRODUCTS; CHLORINATED HYDROCARBONS; ELECTROCHEMICAL REACTOR; HALOGENATED FUMIGANTS; DEHALOGENATION; WATER; TRANSFORMATION; DECOMPOSITION; REGENERATION; THIOSULFATE AB Due to concerns surrounding its ozone depletion potential, there is a need for technologies to capture and destroy methyl bromide (CH3Br) emissions from postharvest fumigations applied to control agricultural pests. Previously, we described a system in which CH3Br fumes vented from fumigation chambers could be captured by granular activated carbon (GAC). The GAC was converted to a cathode by submergence in a high ionic strength solution and connection to the electrical grid, resulting in reductive debromination of the sorbed CH3Br. The GAC bed was drained and dried for reuse to capture and destroy CH3Br fumes from the next fumigation. However, the loose GAC particles and slow kinetics of this primitive electrode necessitated improvements. Here, we report the development of a cathode containing a thin layer of small GAC particles coating carbon cloth as a current distributor. Combining the high sorption potential of GAC for CH3Br with the conductivity of the carbon cloth current distributor, the cathode significantly lowered the total cell resistance and achieved 96% reductive debromination of CH3Br sorbed at 30% by weight to the GAC within IS h at -1 V applied potential vs standard hydrogen electrode, a time scale and efficiency suitable for postharvest fumigations. The cathode exhibited stable performance over 50 CH3Br capture and destruction cycles. Initial cost estimates indicate that this technique could treat CH3Br fumes at similar to$5/kg, roughly one-third of the cost of current alternatives. C1 [Li, Yuanqing; Mitch, William A.] Stanford Univ, Dept Civil & Environm Engn, 473 Via Ortega, Stanford, CA 94305 USA. [Liu, Chong; Cui, Yi] Stanford Univ, Dept Mat Sci & Engn, McCullough Bldg, Stanford, CA 94305 USA. [Walse, Spencer S.] ARS, San Joaquin Valley Agr Sci Ctr, USDA, 9611 South Riverbend Ave, Parlier, CA 93648 USA. [Olver, Ryan; Zilberman, David] Univ Calif Berkeley, Dept Agr & Resource Econ, Giannini Hall, Berkeley, CA 94720 USA. RP Mitch, WA (reprint author), Stanford Univ, Dept Civil & Environm Engn, 473 Via Ortega, Stanford, CA 94305 USA. EM wamitch@stanford.edu FU USDA Agricultural Research Service; USDA Foreign Agricultural Service; California Dried Plum Board under the Technical Assistance for Specialty Crops program [2010-19] FX This research was funded by the USDA Agricultural Research Service as well as the USDA Foreign Agricultural Service and the California Dried Plum Board under the Technical Assistance for Specialty Crops program, agreement #2010-19. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. USDA is an equal opportunity provider and employer. NR 28 TC 0 Z9 0 U1 10 U2 10 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD OCT 18 PY 2016 VL 50 IS 20 BP 11200 EP 11208 DI 10.1021/acs.est.6b03489 PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DZ5MW UT WOS:000385907200049 PM 27611209 ER PT J AU Lu, XL Kracher, B Saur, IML Bauer, S Ellwood, SR Wise, R Yaeno, T Maekawa, T Schulze-Lefert, P AF Lu, Xunli Kracher, Barbara Saur, Isabel M. L. Bauer, Saskia Ellwood, Simon R. Wise, Roger Yaeno, Takashi Maekawa, Takaki Schulze-Lefert, Paul TI Allelic barley MLA immune receptors recognize sequence-unrelated avirulence effectors of the powdery mildew pathogen SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE avirulence effectors; association analysis; R genes; plant-microbe interactions; powdery mildew ID F-SP HORDEI; INTEGRATIVE GENOMICS VIEWER; DISEASE RESISTANCE GENES; MAGNAPORTHE-ORYZAE; PLANT-PATHOGENS; NLR FUNCTIONS; EVOLUTION; WHEAT; LOCUS; CONSERVATION AB Disease-resistance genes encoding intracellular nucleotide-binding domain and leucine-rich repeat proteins (NLRs) are key components of the plant innate immune system and typically detect the presence of isolate-specific avirulence (AVR) effectors from pathogens. NLR genes define the fastest-evolving gene family of flowering plants and are often arranged in gene clusters containing multiple paralogs, contributing to copy number and allele-specific NLR variation within a host species. Barley mildew resistance locus a (Mla) has been subject to extensive functional diversification, resulting in allelic resistance specificities each recognizing a cognate, but largely unidentified, AVR(a) gene of the powdery mildew fungus, Blumeria graminis f. sp. hordei (Bgh). We applied a transcriptome-wide association study among 17 Bgh isolates containing different AVR(a) genes and identified AVR(a1) and AVR(a13), encoding candidate-secreted effectors recognized by Mla1 and Mla13 alleles, respectively. Transient expression of the effector genes in barley leaves or protoplasts was sufficient to trigger Mla1 or Mla13 allele-specific cell death, a hallmark of NLR receptor-mediated immunity. AVR(a1) and AVR(a13) are phylogenetically unrelated, demonstrating that certain allelic MLA receptors evolved to recognize sequence-unrelated effectors. They are ancient effectors because corresponding loci are present in wheat powdery mildew. AVRA1 recognition by barley MLA1 is retained in transgenic Arabidopsis, indicating that AVR(A1) directly binds MLA1 or that its recognition involves an evolutionarily conserved host target of AVR(A1). Furthermore, analysis of transcriptome-wide sequence variation among the Bgh isolates provides evidence for Bgh population structure that is partially linked to geographic isolation. C1 [Lu, Xunli; Kracher, Barbara; Saur, Isabel M. L.; Bauer, Saskia; Maekawa, Takaki; Schulze-Lefert, Paul] Max Planck Inst Plant Breeding Res, Dept Plant Microbe Interact, D-50829 Cologne, Germany. [Ellwood, Simon R.] Curtin Univ, Dept Environm & Agr, Ctr Crop & Dis Management, Bentley, WA 6102, Australia. [Wise, Roger] ARS, Corn Insects & Crop Genet Res, USDA, Ames, IA 50011 USA. [Wise, Roger] Iowa State Univ, Ctr Plant Responses Environm Stresses, Dept Plant Pathol & Microbiol, Ames, IA 50011 USA. [Yaeno, Takashi] Ehime Univ, Fac Agr, Matsuyama, Ehime 7908566, Japan. RP Maekawa, T; Schulze-Lefert, P (reprint author), Max Planck Inst Plant Breeding Res, Dept Plant Microbe Interact, D-50829 Cologne, Germany. EM maekawa@mpipz.mpg.de; schlef@mpipz.mpg.de FU Max-Planck Society; German Research Foundation in the Collaborative Research Centre [SFB670]; Grains Research AMP; Development Corporation [CUR00017, CUR00023 P8]; JSPS KAKENHI Grant Inamori Foundation [JP16K07618] FX We thank L. Chartrain and J. Brown (John Innes Centre) for providing the Bgh isolates DH14, CC1, CC52, CC66, CC88, CC107, and CC148; M. F. Lyngkjaer (University of Copenhagen) for Bgh isolate B103; R. Oliver (Curtin University) for the Bgh isolates Aby, Art, and Will; H. Hisano (Okayama University) for the Bgh isolate OU14; N. Yamaoka (Ehime University) for the Bgh isolate RACE1; and M. Moscou (The Sainsbury Laboratory) for providing the Manchuria NIL lines. The ZmUbi-LUC plasmid, pIPKb002, and R4pGWB4-stop-HSP were kindly provided by R. Panstruga (RWTH Aachen University), J. Kumlehn (Leibniz Institute of Plant Genetics and Crop Plant Research), and N. Mitsuda (National Institute of Advanced Industrial Science and Technology), respectively. We thank C. Pedersen (University of Copenhagen) for helpful suggestions. We also thank S. Haigis and M. Yoshikawa-Maekawa for maintaining the Bgh isolates; P. Kochner for technical assistance; and the Max Planck Genome Centre Cologne for RNA-seq. This work was supported by the Max-Planck Society (X.L., B.K., I. M. L. S., S.B. and P. S.-L.); German Research Foundation in the Collaborative Research Centre Grant SFB670 (to B.K., T.M., and P. S.-L.); Grains Research & Development Corporation Projects CUR00017 and CUR00023 P8 (to S.R.E.); and the JSPS KAKENHI Grant JP16K07618 Inamori Foundation (to T.Y.). NR 80 TC 2 Z9 2 U1 20 U2 20 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD OCT 18 PY 2016 VL 113 IS 42 BP E6486 EP E6495 DI 10.1073/pnas.1612947113 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ1PN UT WOS:000385610400022 PM 27702901 ER PT J AU Maggioli, MF Palmer, MV Thacker, TC Vordermeier, HM McGill, JL Whelan, AO Larsen, MH Jacobs, WR Waters, WR AF Maggioli, Mayara F. Palmer, Mitchell V. Thacker, Tyler C. Vordermeier, Hans Martin McGill, Jodi L. Whelan, Adam O. Larsen, Michelle H. Jacobs, William R., Jr. Waters, W. Ray TI Increased TNF-alpha/IFN-gamma/IL-2 and Decreased TNF-alpha/IFN-gamma Production by Central Memory T Cells Are Associated with Protective Responses against Bovine Tuberculosis Following BCG Vaccination SO FRONTIERS IN IMMUNOLOGY LA English DT Article DE polyfunctional T cells; central memory T cells; bovine tuberculosis; calf model ID CALMETTE-GUERIN VACCINATION; MYCOBACTERIUM-BOVIS; HISTORICAL PERSPECTIVES; ANTIGENS ESAT-6; INFECTED CATTLE; FUSION PROTEIN; EFFECTOR; VACCINES; GENERATION; DIFFERENTIATION AB Central memory T cell (Tcm) and polyfunctional CD4 T cell responses contribute to vaccine-elicited protection with both human and bovine tuberculosis (TB); however, their combined role in protective immunity to TB is unclear. To address this question, we evaluated polyfunctional cytokine responses by CD4 T cell effector/memory populations from bacille Calmette-Guerin (BCG) vaccinated and non-vaccinated calves by flow cytometry prior to and after aerosol challenge with virulent Mycobacterium bovis. Polyfunctional cytokine expression patterns in the response by Tcm, effector memory, and effector T cell subsets were similar between BCG-vaccinated and M. bovis-infected calves, only differing in magnitude (i.e., infected > vaccinated). BCG vaccination, however, did alter the kinetics of the ensuing response to virulent M. bovis infection. Early after challenge (3 weeks post-infection), non-vaccinates had greater antigen-specific interferon-7 (IFN-7)/tumor necrosis factor-alpha (TNIF-alpha) and lesser IFN-7/TNF-alpha/IL-2 responses by Tcm cells than did vaccinated animals. Importantly, these differences were also associated with mycobacterial burden upon necropsy. Polyfunctional responses to ESAT-6:CFP10 (antigens not synthesized by BCG strains) were detected in memory subsets, as well as in effector cells, as early as 3 weeks after challenge. These findings suggest that cell fate divergence may occur early after antigen priming in the response to bovine TB and that memory and effector T cells may expand concurrently during the initial phase of the immune response. In summary, robust IFN-gamma/TNIF-alpha response by Tom cells is associated with greater mycobacterial burden, while IFN-7/INF-alpha/IL-2 response by Tcm cells are indicative of a protective response to bovine TB. C1 [Maggioli, Mayara F.; Palmer, Mitchell V.; Thacker, Tyler C.; Waters, W. Ray] Natl Anim Dis Ctr, Infect Bacterial Dis Livestock Res Unit, Ames, IA 50010 USA. [Maggioli, Mayara F.] Iowa State Univ, Coll Vet Med, Dept Vet Pathol, Imbio, Ames, IA 50011 USA. [Vordermeier, Hans Martin] Anim & Plant Hlth Agcy, TB Res Grp, Addlestone, Surrey, England. [McGill, Jodi L.] Kansas State Univ, Coll Vet Med, Dept Diagnost Med & Pathol, Manhattan, KS 66506 USA. [Whelan, Adam O.] Def Sci & Technol Lab, Porton Down, Wilts, England. [Larsen, Michelle H.; Jacobs, William R., Jr.] Albert Einstein Coll Med, Dept Microbiol & Immunol, Bronx, NY 10467 USA. RP Maggioli, MF (reprint author), Natl Anim Dis Ctr, Infect Bacterial Dis Livestock Res Unit, Ames, IA 50010 USA.; Maggioli, MF (reprint author), Iowa State Univ, Coll Vet Med, Dept Vet Pathol, Imbio, Ames, IA 50011 USA. EM mayaramaggioli@gmail.com RI Vordermeier, H Martin/C-6936-2011; APHA, Staff publications/E-6082-2010 FU AFRI Competitive Grant [2011-67015-30736]; NIFA-USDA-NIH program Dual Purpose; Dual Benefit Research in Biomedicine and Agriculture Using Agriculturally Important Domestic Animals FX This study was partially supported by AFRI Competitive Grant #2011-67015-30736 to WW and MP from the NIFA-USDA-NIH program Dual Purpose with Dual Benefit Research in Biomedicine and Agriculture Using Agriculturally Important Domestic Animals. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. NR 85 TC 0 Z9 0 U1 6 U2 6 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-3224 J9 FRONT IMMUNOL JI Front. Immunol. PD OCT 17 PY 2016 VL 7 AR 421 DI 10.3389/fimmu.2016.00421 PG 18 WC Immunology SC Immunology GA DY8XX UT WOS:000385416800002 PM 27799930 ER PT J AU Chiu, CJ Chang, ML Taylor, A AF Chiu, Chung-Jung Chang, Min-Lee Taylor, Allen TI Associations between Periodontal Microbiota and Death Rates SO SCIENTIFIC REPORTS LA English DT Article ID NUTRITION EXAMINATION SURVEY; ALL-CAUSE MORTALITY; NATIONAL-HEALTH; PORPHYROMONAS-GINGIVALIS; CARDIOVASCULAR-DISEASE; DIABETES-MELLITUS; OXIDATIVE STRESS; SERUM ANTIBODY; ORAL HYGIENE; NHANES-III AB It is conceived that specific combinations of periodontal bacteria are associated with risk for the various forms of periodontitis. We hypothesized that such specificity is also related to human cause-specific death rates. We tested this hypothesis in a representative sample of the US population followed for a mean duration of 11 years and found that two specific patterns of 21 serum antibodies against periodontal bacteria were significantly associated with increased all-cause and/or diabetes-related mortalities. These data suggested that specific combinations of periodontal bacteria, even without inducing clinically significant periodontitis, may have a significant impact on human cause-specific death rates. Our findings implied that increased disease and mortality risk could be transmittable via the transfer of oral microbiota, and that developing personalized strategies and maintaining healthy oral microbiota beyond protection against periodontitis would be important to manage the risk. C1 [Chiu, Chung-Jung; Chang, Min-Lee; Taylor, Allen] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Chiu, CJ (reprint author), Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM CJ.Chiu@tufts.edu FU National Institutes of Health [RO1EY021826, RO1EY013250, RO1EY021212]; United States Department of Agriculture [1950-5100-060-01A] FX Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views or policies of the U.S. Department of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government. Financial support for this project has been provided by the RO1EY021826 (C.-J.C.), RO1EY013250 and RO1EY021212 (A.T.) from the National Institutes of Health, and the United States Department of Agriculture under agreements, 1950-5100-060-01A. We thank Yu-Hsuan Chiu for her help with proofreading this manuscript. NR 53 TC 0 Z9 0 U1 4 U2 4 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD OCT 17 PY 2016 VL 6 AR 35428 DI 10.1038/srep35428 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DY9LI UT WOS:000385454800001 PM 27748442 ER PT J AU Ren, JS Murphy, G Fan, JH Dawsey, SM Taylor, PR Selhub, J Qiao, YL Abnet, CC AF Ren, Jiansong Murphy, Gwen Fan, Jinhu Dawsey, Sanford M. Taylor, Philip R. Selhub, Jacob Qiao, Youlin Abnet, Christian C. TI Prospective study of serum B vitamins levels and oesophageal and gastric cancers in China SO SCIENTIFIC REPORTS LA English DT Article ID NUTRITION INTERVENTION; GENERAL-POPULATION; DIETARY-FOLATE; HUMAN-PLASMA; RISK; RIBOFLAVIN; COHORT; SUPPLEMENTATION; TRIAL; HOMOCYSTEINE AB B vitamins play an essential role in DNA synthesis and methylation, and may protect against oesophageal and gastric cancers. In this case-cohort study, subjects were enrolled from the General Population Nutrition Intervention Trial in Linxian, China. Subjects included 498 oesophageal squamous cell carcinomas (OSCCs), 255 gastric cardia adenocarcinomas (GCAs), and an age-and sex-matched sub-cohort of 947 individuals. Baseline serum riboflavin, pyridoxal phosphate (PLP), folate, vitamin B12, and flavin mononucleotide (FMN) were measured for all subjects. We estimated the associations with Cox proportional hazard models, with adjustment for potential confounders. Compared to those in the lowest quartile of serum riboflavin, those in the highest had a 44% lower risk of OSCC (HR: 0.56, 95% CI: 0.41 to 0.75). Serum vitamin B12 as a continuous variable was observed to be significantly inversely associated with OSCC (HR: 0.95, 95% CI: 0.89 to 1.01, P for score test = 0.041). Higher serum FMN levels were significantly associated with increased risk of OSCC (HR: 1.08, 95% CI: 1.01 to 1.16) and GCA (HR: 1.09, 95% CI: 1.00 to 1.20). Our study prompted that B vitamins have the potential role as chemopreventive agents for upper gastrointestinal cancers. C1 [Ren, Jiansong] Chinese Acad Med Sci, Natl Canc Ctr, Canc Hosp, Program Off Canc Screening Urban China, Beijing, Peoples R China. [Ren, Jiansong; Fan, Jinhu; Qiao, Youlin] Peking Union Med Coll, Beijing, Peoples R China. [Ren, Jiansong; Murphy, Gwen; Dawsey, Sanford M.; Taylor, Philip R.; Abnet, Christian C.] NCI, Div Canc Epidemiol & Genet, NIH, Bethesda, MD 20892 USA. [Fan, Jinhu; Qiao, Youlin] Chinese Acad Med Sci, Canc Hosp, Natl Canc Ctr, Dept Epidemiol, Beijing, Peoples R China. [Selhub, Jacob] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Qiao, YL (reprint author), Peking Union Med Coll, Beijing, Peoples R China.; Abnet, CC (reprint author), NCI, Div Canc Epidemiol & Genet, NIH, Bethesda, MD 20892 USA.; Qiao, YL (reprint author), Chinese Acad Med Sci, Canc Hosp, Natl Canc Ctr, Dept Epidemiol, Beijing, Peoples R China. EM qiaoy@cicams.ac.cn; abnetc@mail.nih.gov RI Abnet, Christian/C-4111-2015 OI Abnet, Christian/0000-0002-3008-7843 FU Intramural Research Program of the Division of Cancer Epidemiology and Genetics of the National Cancer Institute, NIH; NIH [N01-SC-91030, HHSN261200477001C]; National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences FX This work was supported in part by the Intramural Research Program of the Division of Cancer Epidemiology and Genetics of the National Cancer Institute, NIH, and in part by NIH contracts N01-SC-91030 and HHSN261200477001C with the National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences. NR 27 TC 0 Z9 0 U1 3 U2 3 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD OCT 17 PY 2016 VL 6 AR 35281 DI 10.1038/srep35281 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DY8CI UT WOS:000385355700002 PM 27748414 ER PT J AU Mukhopadhyay, S Sokorai, K Ukuku, D Fan, XT Juneja, V Sites, J Cassidy, J AF Mukhopadhyay, Sudarsan Sokorai, Kimberly Ukuku, Dike Fan, Xuetong Juneja, Vijay Sites, Joseph Cassidy, Jennifer TI Inactivation of Salmonella enterica and Listeria monocytogenes in cantaloupe puree by high hydrostatic pressure with/without added ascorbic acid SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Cantaloupe puree; High hydrostatic pressure processing; Salmonella enterica; Listeria monocytogenes; Antimicrobial ID ESCHERICHIA-COLI O157H7; FRESH-CUT PIECES; STRAWBERRY PUREE; FRUIT JUICES; SUBLETHAL INJURY; CHERRY TOMATO; STORAGE; APPLE; SURVIVAL; RIND AB The objective of this research was to evaluate and develop a method for inactivation of Salmonella enterica and Listeria monocytogenes in cantaloupe puree (CP) by high hydrostatic pressure (HHP). Cantaloupe being the most netted varieties of melons presents a greater risk of pathogen transmission. Freshly prepared CP with or without 0.1% ascorbic acid (AA) was inoculated with a bacterial cocktail composed of a three serotype mixture of S. enterica (S. Poona, S. Newport H1275 and S. Stanley H0558) and a mixture of three strains of L. monocytogenes (Scott A, 43256 and 51742) to a population of ca. 10(8) CFU/g. Double sealed and double bagged inoculated CP (ca. 5 g) were pressure treated at 300,400 and 500 MPa at 8 degrees C and 15 degrees C for 5 min. Data indicated increased inactivation of both Salmonella and Listeria spp. with higher pressure. Log reduction for CP at 300 MPa, 8 degrees C for 5 min was 2.4 +/- 0.2 and 1.6 +/- 0.5 log CFU/g for Salmonella and Listeria, respectively. Survivability of the pathogens was significantly compromised at 400 MPa and 8 degrees C, inactivating 4.5 +/- 0.3 log CFU/g of Salmonella and 3.0 +/- 0.4 log CFU/g of Listeria spp. Complete inactivation of the pathogens in the puree (log reduction >6.7 log CFU/g), with or without AA, was achieved when the pressure was further increased to 500 MPa, except that for Listeria containing no AA at 8 degrees C. Listeria presented higher resistance to pressure treatment compared to Salmonella spp. Initial temperatures (8 and 15 degrees C) had no significant influence on Salmonella log reductions. Log reduction of pathogens increased but not significantly with increase of temperature. AA did not show any significant antimicrobial activity. Viable counts were about 0.2-0.4 log CFU/g less in presence of 0.1% AA. These data validate that HHP can be used as an effective method for decontamination of cantaloupe puree. Published by Elsevier B.V. C1 [Mukhopadhyay, Sudarsan; Sokorai, Kimberly; Fan, Xuetong; Juneja, Vijay] ARS, Residue Chem & Predict Microbiol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Ukuku, Dike; Sites, Joseph; Cassidy, Jennifer] ARS, Food Safety Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Mukhopadhyay, S (reprint author), ARS, Residue Chem & Predict Microbiol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM Sudarsan.mukhopadhyay@ars.usda.gov; Kimberly.sokorai@ars.usda.gov; Dike.ukuku@ars.usda.gov; Xuetong.fan@ars.usda.gov; Vijay.juneja@ars.usda.gov; Joseph.sites@ars.usda.gov; jennifer.cassidy@ars.usda.gov FU USDA through ARS National Program [108]; ARS CRIS project [1935-41000-092] FX The authors thank Jia Zhen for the help with high pressure experiments and Dr. John Phillips for assistance with statistical data analysis. This study was funded by the USDA through ARS National Program 108, ARS CRIS project 1935-41000-092. NR 59 TC 1 Z9 1 U1 20 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD OCT 17 PY 2016 VL 235 BP 77 EP 84 DI 10.1016/j.ijfoodmicro.2016.07.007 PG 8 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA DW7IB UT WOS:000383823300010 PM 27441819 ER PT J AU Sayols-Baixeras, S Subirana, I Lluis-Ganella, C Civeira, F Roquer, J Do, AN Absher, D Cenarro, A Munoz, D Soriano-Tarraga, C Jimenez-Conde, J Ordovas, JM Senti, M Aslibekyan, S Marrugat, J Arnett, DK Elosua, R AF Sayols-Baixeras, S. Subirana, I. Lluis-Ganella, C. Civeira, F. Roquer, J. Do, A. N. Absher, D. Cenarro, A. Munoz, D. Soriano-Tarraga, C. Jimenez-Conde, J. Ordovas, J. M. Senti, M. Aslibekyan, S. Marrugat, J. Arnett, D. K. Elosua, R. TI Identification and validation of seven new loci showing differential DNA methylation related to serum lipid profile: an epigenome-wide approach. The REGICOR study SO HUMAN MOLECULAR GENETICS LA English DT Article ID FAMILIAL COMBINED HYPERLIPIDEMIA; DENSITY-LIPOPROTEIN CHOLESTEROL; THIOREDOXIN INTERACTING PROTEIN; BODY-MASS INDEX; WAIST CIRCUMFERENCE; DIABETES-MELLITUS; LOWERING DRUGS; GENE PROMOTER; DIET NETWORK; BLOOD-LIPIDS AB Lipid traits (total, low-density and high-density lipoprotein cholesterol, and triglycerides) are risk factors for cardiovascular disease. DNA methylation is not only an inherited but also modifiable epigenetic mark that has been related to cardiovascular risk factors. Our aim was to identify loci showing differential DNA methylation related to serum lipid levels. Blood DNA methylation was assessed using the Illumina Human Methylation 450 BeadChip. A two-stage epigenome-wide association study was performed, with a discovery sample in the REGICOR study (n = 645) and validation in the Framingham Offspring Study (n = 2,542). Fourteen CpG sites located in nine genes (SREBF1, SREBF2, PHOSPHO1, SYNGAP1, ABCG1, CPT1A, MYLIP, TXNIP and SLC7A11) and 2 intergenic regions showed differential methylation in association with lipid traits. Six of these genes and 1 intergenic region were new discoveries showing differential methylation related to total cholesterol (SREBF2), HDLcholesterol (PHOSPHO1, SYNGAP1 and an intergenic region in chromosome 2) and triglycerides (MYLIP, TXNIP and SLC7A11). These CpGs explained 0.7%, 9.5% and 18.9% of the variability of total cholesterol, HDL cholesterol and triglycerides in the Framingham Offspring Study, respectively. The expression of the genes SREBF2 and SREBF1 was inversely associated with methylation of their corresponding CpGs (P-value = 0.0042 and 0.0045, respectively) in participants of the GOLDN study ( n = 98). In turn, SREBF1 expression was directly associated with HDL cholesterol ( P-value = 0.0429). Genetic variants in SREBF1, PHOSPHO1, ABCG1 and CPT1A were also associated with lipid profile. Further research is warranted to functionally validate these new loci and assess the causality of new and established associations between these differentially methylated loci and lipid metabolism. C1 [Sayols-Baixeras, S.; Subirana, I.; Lluis-Ganella, C.; Marrugat, J.; Elosua, R.] Hosp del Mar, Med Res Inst, IMIM, Cardiovasc Epidemiol & Genet Res Grp, Barcelona, Catalonia, Spain. [Sayols-Baixeras, S.] UPF, Barcelona, Catalonia, Spain. [Subirana, I.] CIBER Epidemiol & Publ Hlth, Barcelona, Catalonia, Spain. [Civeira, F.; Cenarro, A.] Hosp Univ Miguel Servet, Inst Invest Sanitaria Aragon, Unidad Clin & Invest Lipidos & Arteriosclerosis, Zaragoza, Spain. [Roquer, J.; Soriano-Tarraga, C.; Jimenez-Conde, J.] Hosp del Mar, Med Res Inst, IMIM, Neurovasc Res Grp,Dept Neurol, Barcelona 08003, Catalonia, Spain. [Do, A. N.; Aslibekyan, S.] Univ Alabama Birmingham, Dept Epidemiol, Birmingham, AL USA. [Absher, D.] Hudson Alpha Inst Biotechnol, Huntsville, AL USA. [Munoz, D.] Hosp del Mar, Med Res Inst, IMIM, Cardiovasc Risk & Nutr Res Grp, Barcelona, Catalonia, Spain. [Ordovas, J. M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Senti, M.] Pompeu Fabra Univ, Dept Expt & Hlth Sci, Barcelona, Catalonia, Spain. [Arnett, D. K.] Univ Kentucky, Coll Publ Hlth, Deans Off, Lexington, KY USA. RP Elosua, R (reprint author), Hosp del Mar, IMIM, Med Res Inst, Dr Aiguader 80, Barcelona 08003, Catalonia, Spain. EM relosua@imim.es FU Agencia de Gestio Ajuts Universitaris de Recerca [2014 SGR 240]; Spanish Ministry of Economy through the Carlos III Health Institute [ISCIII-FIS-FEDER-ERDF PI12-00232, PI12-01238, PI11-01801, PI08-1327, PI05-1251, PI05-1297, PI02-0471, FIS99/0013-01, FIS96/0026-01, FIS93/0568, FIS92/0009-05]; Red de Investigacion Cardiovascular [RD12/0042/0013, RD12/0042/0020, RD12/0042/0055, RD12/0042/0061]; Instituto de Salud Carlos III FEDER [IFI14/00007]; Daniel Bravo Andreu Private Foundation; US National Institute of Health (NIH)/National Heart, Lung and Blood Institutes [R01HL104135, U01HL72524] FX This work was supported by the following sources: Agencia de Gestio Ajuts Universitaris de Recerca [2014 SGR 240]; the Spanish Ministry of Economy through the Carlos III Health Institute [ISCIII-FIS-FEDER-ERDF PI12-00232, PI12-01238, PI11-01801, PI08-1327, PI05-1251, PI05-1297, PI02-0471, FIS99/0013-01, FIS96/0026-01, FIS93/0568, FIS92/0009-05], and the Red de Investigacion Cardiovascular [RD12/0042/0013, RD12/0042/0020, RD12/0042/0055, RD12/0042/0061]. S.S-B. was funded by a contract from Instituto de Salud Carlos III FEDER [IFI14/00007] and Daniel Bravo Andreu Private Foundation.; GOLDN: The GOLDN study (AND, DA, JO, SA, DKA) was funded by the US National Institute of Health (NIH)/National Heart, Lung and Blood Institutes (http://www.nhlbi.nih.gov) grants R01HL104135 and U01HL72524. NR 52 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0964-6906 EI 1460-2083 J9 HUM MOL GENET JI Hum. Mol. Genet. PD OCT 15 PY 2016 VL 25 IS 20 BP 4556 EP 4565 DI 10.1093/hmg/ddw285 PG 10 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA EN1ZM UT WOS:000395809100016 PM 28173150 ER PT J AU Dubey, JP Brown, J Ternent, M Verma, SK Hill, DE Cerqueira-Cezar, CK Kwok, OCH Calero-Bernal, R Humphreys, JG AF Dubey, Jitender P. Brown, Justin Ternent, Mark Verma, Shiv K. Hill, Dolores E. Cerqueira-Cezar, Camila K. Kwok, Oliver C. H. Calero-Bernal, Rafael Humphreys, Jan G. TI Seroepidemiologic study on the prevalence of Toxoplasma gondii and Trichinella spp. infections in black bears (Ursus americanus) in Pennsylvania, USA SO VETERINARY PARASITOLOGY LA English DT Article DE Toxoplasma gondii; Trichinella spp.; ELISA; Modified agglutination test; Epidemiology; Black bear (Ursus americanus) ID UNITED-STATES; SEROLOGIC TESTS; TRICHINOSIS; MURRELLI; CALIFORNIA; SPIRALIS; WILDLIFE AB The protozoan Toxoplasma gondii and the metazoan Trichinella spp. infect virtually all warm-blooded animals, including birds, humans, livestock, and marine mammals. Both parasitic infections can cause serious illness in human beings and can be acquired by ingesting under-cooked meat harboring infective stages. Approximately 3500 black bears (Ursus americanus) are legally-harvested each year in Pennsylvania, USA during the November hunting season. Among animals found infected with T. gondii, the prevalence of T. gondii is the highest among black bears in the USA; however, little is currently known of epidemiology of toxoplasmosis in this host species. Serum samples were collected during the winters of 2015 and 2016 from adult female bears and their nursing cubs or yearlings while they were still in their dens. Additionally, archived sera from bear samples collected throughout the year, including hunter-harvested bears in November and trapped bears in the summer, were serologically tested. Antibodies to T. gondii were assayed by the modified agglutination test (MAT, cut-off 1:25) and antibodies to Trichinella spp. were assayed using a commercial enzyme-linked immunosorbent assay (ELISA). Overall, T. gondii antibodies were found in 87.6% (206/235) of adults, and 44.1% (30/68) of yearlings. In March 2015/2016 sampling, antibodies to T. gondii were found in 94% (30/32) adult female bears while in their den. Antibodies were detected in 5% (3/66) of the nursing cubs in the dens of these sows. One positive cub had a MAT titer of 1:160 and two were positive at the 1:25 dilution but not at 1:50. The adult females of these cubs had MAT titers ranging from 1:400 to 1:3200. Antibodies to Trichinella spp. were found in 3% (6/181) of adults and 3.6% (1/28) of yearlings; these 7 bears were also seropositive for T. gondii. No antibodies to Trichinella spp. were detected in the sera of 44 nursing cubs tested. The finding of T. gondii antibodies in only 3 of 66 cubs, and higher antibody titers in their respective sows indicates that the colostrally-acquired antibodies wane to undetectable levels by 8-10 weeks, while the cubs are still in the den. The results indicate that there is no transplacental transmission of T. gondii, that antibodies acquired from colostrum are largely undetectable by the time cubs emerge from the den, and nearly that 50% of bears acquire infection postnatally by 10 months of age. This is the first report of disappearance of transcolostral antibodies of any infection in bears. (C) 2016 Published by Elsevier B.V. C1 [Dubey, Jitender P.; Verma, Shiv K.; Hill, Dolores E.; Cerqueira-Cezar, Camila K.; Kwok, Oliver C. H.; Calero-Bernal, Rafael] USDA ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Brown, Justin; Ternent, Mark] Penn Game Commiss, 2001 Elmerton Ave, Harrisburg, PA 17110 USA. [Humphreys, Jan G.] Indiana Univ Penn, Dept Biol, Indiana, PA 15705 USA. RP Dubey, JP (reprint author), USDA, BARC, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov NR 30 TC 0 Z9 0 U1 3 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD OCT 15 PY 2016 VL 229 BP 76 EP 80 DI 10.1016/j.vetpar.2016.09.013 PG 5 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA ED0RI UT WOS:000388549200013 PM 27809983 ER PT J AU Tuo, WB Li, L Lv, YJ Carrillo, J Brown, D Davis, WC Song, JZ Zarlenga, D Xiao, ZG AF Tuo, Wenbin Li, Lei Lv, Yingjun Carrillo, Jose Brown, Dedra Davis, William C. Song, Jiuzhou Zarlenga, Dante Xiao, Zhengguo TI Abomasal mucosal immune responses of cattle with limited or continuous exposure to pasture-borne gastrointestinal nematode parasite infection SO VETERINARY PARASITOLOGY LA English DT Article DE No pasture exposure (NPE); Limited pasture exposure (LPE); Continuous pasture exposure (CPE); Cattle; Gastrointestinal nematode parasite; Ostertagia ostertagi; B cells; T cells ID OSTERTAGIA-OSTERTAGI INFECTION; FATTY-ACID PROFILES; GRAIN-FED CATTLE; RED MEAT; FED BEEF; QUALITY; CALVES; GRASS; RESISTANCE; RUMINANTS AB It has been well documented that cattle raised on pasture are slow in weight gain when compared to those fed with grain. Inflammation in the digestive system commonly caused by pasture-transmitted gastrointestinal (GI) nematode parasites that could negatively impact feed conversion has never been compared in cattle raised with no pasture exposure (NPE, uninfected), limited pasture exposure (LPE, exposure until weaning), or continuous pasture exposure (CPE, life time exposure). In the present study, the abomasal mucosa] immune responses and inflammation of LPE and CPE cattle were investigated. Our results indicate that CPE cattle displayed inflamed abomasa with enlarged draining lymph nodes, the presence of Ostertagia ostertagi larvae and higher levels of Ostertagia-specific antibodies in circulation. The level of B cells was elevated in the abomasal mucosa in the presence (nodular) or absence (non-nodular) of Ostertagia-specific pathology, where B cells were 4-fold higher in the nodular mucosa. Foxp3(+) CD4T cells were also noticeably elevated in both the abomasal mucosa and blood, but were only slightly higher in non-nodular mucosa than in the nodular mucosa of CPE animals. In contrast, LPE animals presented no enlargement of abomasal draining lymph nodes and exhibited little to no immune cell infiltration in the abomasal mucosa. Further, CPE animals had higher numbers of mucosal mast cells when compared to LPE animals, though mucosal mast cells were high in all animals. Overall, CPE cattle displayed significantly higher levels of inflammation and pathology in their abomasa and may explain in part slowed weight gain relative to LPE animals. The results of this study emphasize the need for GI nematode parasite control in CPE animals and development and application of vaccines which are compatible with the organic cattle production system. Published by Elsevier B.V. C1 [Tuo, Wenbin; Brown, Dedra; Zarlenga, Dante] USDA ARS, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Li, Lei; Lv, Yingjun; Carrillo, Jose; Song, Jiuzhou; Xiao, Zhengguo] Univ Maryland, Dept Avian & Anim Sci, College Pk, MD 20742 USA. [Davis, William C.] Washington State Univ, Dept Vet Microbiol & Pathobiol, Pullman, WA 99164 USA. [Brown, Dedra] Texas A&M Univ, Dept Anim Rangeland & Wildlife Sci, 700 Univ Blvd, Kingsville, TX 78363 USA. RP Tuo, WB (reprint author), USDA ARS, Anim Parasit Dis Lab, Beltsville, MD 20705 USA.; Xiao, ZG (reprint author), Univ Maryland, Dept Avian & Anim Sci, College Pk, MD 20742 USA. EM Wenbin.tuo@ars.usda.gov; xiao0028@umd.edu FU USDA/ARS; USDA NIFA [GRANT11885997]; University of Maryland; STEP UP/USDA Project of the Texas A&M University System FX The authors are grateful to Mr. Eliseo Miramontes and Ms. Marsha Campbell of USDA/ARS for excellent technical assistance. Research was supported by funds from USDA/ARS (WT, DZ), USDA NIFA GRANT11885997 (ZX), and USDA/ARS Specific Cooperative Agreement with University of Maryland (WT, ZX). Ms. Dedra Brown was a summer student and supported by the STEP UP/USDA Project of the Texas A&M University System. Authors are grateful to the inspiring discussions with and/or critical reviews by Drs. Raymond Fetterer and Benjamin Rosenthal of APDL/BARC, USDA/ARS. NR 42 TC 0 Z9 0 U1 4 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD OCT 15 PY 2016 VL 229 BP 118 EP 125 DI 10.1016/j.vetpar.2016.10.005 PG 8 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA ED0RI UT WOS:000388549200020 PM 27809966 ER PT J AU Payabyab, EC Balasubramaniam, S Edgerly, M Velarde, M Merino, MJ Venkatesan, AM Leuva, H Litman, T Bates, SE Fojo, T AF Payabyab, Eden C. Balasubramaniam, Sanjeeve Edgerly, Maureen Velarde, Margarita Merino, Maria J. Venkatesan, Aradhana M. Leuva, Harshraj Litman, Thomas Bates, Susan E. Fojo, Tito TI Adrenocortical Cancer: A Molecularly Complex Disease Where Surgery Matters SO CLINICAL CANCER RESEARCH LA English DT Article ID ADRENAL-CORTICAL CARCINOMA; LONG-TERM SURVIVAL; LAPAROSCOPIC ADRENALECTOMY; GENOMIC CHARACTERIZATION; ONCOLOGIC OUTCOMES; ADJUVANT MITOTANE; PHASE-II; STAGE-I; THERAPY; TUMORS AB The development of new therapies has lagged behind for rare cancers without defined therapeutic targets. Adrenocortical cancer is no exception. Mitotane, an older agent considered "adrenolytic," is used both to control symptoms in advanced disease and as adjuvant therapy after surgical resection. Molecular characterization of adrenocortical cancer has deepened our understanding of this genetically complex disease while identifying subgroups whose importance remains to be determined. Unfortunately, such studies have yet to demonstrate a therapeutic target for drug development, and to date, no targeted therapy has achieved meaningful outcomes. Consequently, first-line therapy for metastatic disease remains a combination regimen of etoposide, doxorubicin, and cisplatinum established in a randomized clinical trial. In addition to evaluating recent studies in adrenocortical cancer, we raise one critical clinical issue-the risk of peritoneal dissemination following laparoscopic resection of adrenocortical cancer. In a retrospective case series of 267 patients referred to the NCI for the treatment of recurrent or advanced adrenocortical cancer, we found extensive peritoneal dissemination in 25 of the 45 patients (55.6%) who had undergone laparoscopic resection, compared with only 7 of the 222 patients (3%) who had undergone an open resection (P < 0.0001). Although this has been debated in the literature, our data argue for an end to laparoscopic resection of adrenocortical cancers to avoid peritoneal dissemination, a complication of laparoscopy that is uniformly fatal. (C) 2016 AACR. C1 [Payabyab, Eden C.] NCI, Surg Branch, NIH, Bldg 10, Bethesda, MD 20892 USA. [Payabyab, Eden C.] NCI, Thorac & GI Oncol Branch, NIH, Bethesda, MD 20892 USA. [Balasubramaniam, Sanjeeve] USDA, Div Oncol Prod 1, OHOP, CDER, Silver Spring, MD USA. [Edgerly, Maureen; Velarde, Margarita] NCI, Med Oncol Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Merino, Maria J.] NCI, Pathol Lab, Ctr Canc Res, NIH, Bldg 10, Bethesda, MD 20892 USA. [Venkatesan, Aradhana M.] Univ Texas MD Anderson Canc Ctr, Dept Diagnost Radiol, Div Diagnost Imaging, Houston, TX 77030 USA. [Leuva, Harshraj; Bates, Susan E.; Fojo, Tito] James J Peters Vet Adm Med Ctr, Bronx, NY USA. [Litman, Thomas] Univ Copenhagen, Med Biostat, Copenhagen, Denmark. [Bates, Susan E.; Fojo, Tito] Columbia Univ, Dept Med, Med Ctr, Div Med Oncol, New York, NY 10032 USA. RP Fojo, T (reprint author), Columbia Univ, Dept Med, Med Ctr, Div Med Oncol, New York, NY 10032 USA.; Fojo, T (reprint author), James J Peters VA Med Ctr, Bronx, NY 10468 USA. EM atf2116@cumc.columbia.edu OI Balasubramaniam, Sanjeeve/0000-0002-0643-2117 NR 57 TC 2 Z9 2 U1 2 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 1078-0432 EI 1557-3265 J9 CLIN CANCER RES JI Clin. Cancer Res. PD OCT 15 PY 2016 VL 22 IS 20 BP 4989 EP 5000 DI 10.1158/1078-0432.CCR-16-1570 PG 12 WC Oncology SC Oncology GA DZ1WP UT WOS:000385632700007 PM 27742785 ER PT J AU Schell, CJ Young, JK Lonsdorf, EV Mateo, JM Santymire, RM AF Schell, Christopher J. Young, Julie K. Lonsdorf, Elizabeth V. Mateo, Jill M. Santymire, Rachel M. TI Olfactory attractants and parity affect prenatal androgens and territoriality of coyote breeding pairs SO PHYSIOLOGY & BEHAVIOR LA English DT Article DE Androgens; Coyotes; Glucocorticoids; Parity; Personality; Repeatability ID AFRICAN WILD DOGS; CONSISTENT INDIVIDUAL-DIFFERENCES; FEMALE ZEBRA FINCHES; COTTON-TOP TAMARINS; MALE HOUSE SPARROWS; CANIS-LATRANS; GLUCOCORTICOID LEVELS; TESTOSTERONE LEVELS; SOCIAL-DOMINANCE; ETHIOPIAN WOLVES AB Hormones are fundamental mediators of personality traits intimately linked with reproductive success. Hence, alterations to endocrine factors may dramatically affect individual behavior that has subsequent fitness consequences. Yet it is unclear how hormonal or behavioral traits change with environmental stressors or over multiple reproductive opportunities, particularly for biparental fauna. To simulate an environmental stressor, we exposed captive coyote (Canis latrans) pairs to novel coyote odor attractants (i.e. commercial scent lures) mid gestation to influence territorial behaviors, fecal glucocorticoid (FGMs) and fecal androgen metabolites (FAMs). In addition, we observed coyote pairs as first-time and experienced breeders to assess the influence of parity on our measures. Treatment pairs received the odors four times over a 20-day period, while control pairs received water. Odor-treated pairs scent-marked (e.g. urinated, ground scratched) and investigated odors more frequently than control pairs, and had higher FAMs when odors were provided. Pairs had higher FAMs as first-time versus experienced breeders, indicating that parity also affected androgen production during gestation. Moreover, repeatability in scent-marking behaviors corresponded with FGMs and FAMs, implying that coyote territoriality during gestation is underpinned by individually-specific hormone profiles. Our results suggest coyote androgens during gestation are sensitive to conspecific olfactory stimuli and prior breeding experience. Consequently, fluctuations in social or other environmental stimuli as well as increasing parity may acutely affect coyote traits essential to reproductive success. (C) 2016 Elsevier Inc All rights reserved. C1 [Schell, Christopher J.; Mateo, Jill M.; Santymire, Rachel M.] Univ Chicago, Comm Evolutionary Biol, 1025 E 57th St,Culver Hall 402, Chicago, IL 60637 USA. [Young, Julie K.] Utah State Univ, USDA WS NWRC, Predator Res Facil, Dept Wildland Resources, USU BNR 163, Logan, UT 84322 USA. [Lonsdorf, Elizabeth V.] Franklin & Marshall Coll, Dept Psychol, POB 3003, Lancaster, PA 17603 USA. [Santymire, Rachel M.] Lincoln Pk Zoo, Dept Conservat & Sci, 2001 N Clark St, Chicago, IL 60614 USA. RP Schell, CJ (reprint author), USDA APHIS WS NWRC, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM cjschell@colostate.edu OI Schell, Christopher/0000-0002-2073-9852 FU University of Chicago Hinds Fund; GAANN Fellowship; United Negro College Fund (UNCF)-Merck Fellowship; National Science Foundation (NSF); U.S. Department of Agriculture Wildlife Services-NWRC FX We would like to thank B. Patterson and T. Price for extensive and valuable feedback on this manuscript, as well as members of the Mateo and Santymire laboratories. This study would not have been possible without the input, time, and animal expertise of S. Brummer, J. Schultz, M. Davis, E. Stevenson, and other staff at the Wildlife Services National Wildlife Research Center's Predator Research Facility in Millville, UT, USA. Special thanks to D. Schell, B. Flack, and other volunteers for providing data collection assistance, behavioral observations, and laboratory analyses. Additional thanks to the University of Chicago Hinds Fund and GAANN Fellowship, the United Negro College Fund (UNCF)-Merck Fellowship, the National Science Foundation (NSF), and U.S. Department of Agriculture Wildlife Services-NWRC as funding sources for this endeavor. This work was performed with the approval of the Institutional Animal Care and Use Committees (IACUC) at the University of Chicago (protocol no. 72184), the NWRC (protocol no. QA-1818), and the Lincoln Park Zoo Research Committee. Mention of companies or commercial products does not imply recommendation or endorsement by USDA over others not mentioned. USDA neither guarantees nor warrants the standard of any product mentioned. Product names are mentioned solely to report factually on available data and to provide specific information. NR 114 TC 0 Z9 0 U1 17 U2 17 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0031-9384 J9 PHYSIOL BEHAV JI Physiol. Behav. PD OCT 15 PY 2016 VL 165 BP 43 EP 54 DI 10.1016/j.physbeh.2016.06.038 PG 12 WC Psychology, Biological; Behavioral Sciences SC Psychology; Behavioral Sciences GA DY0LS UT WOS:000384788900007 PM 27378509 ER PT J AU Krishnan, S Tryon, RR Horn, WF Welch, L Keim, NL AF Krishnan, Sridevi Tryon, Rebecca R. Horn, William F. Welch, Lucas Keim, Nancy L. TI Estradiol, SHBG and leptin interplay with food craving and intake across the menstrual cycle SO PHYSIOLOGY & BEHAVIOR LA English DT Article DE Leptin; Estradiol; Food intake; Sex hormones; Craving ID HORMONE-BINDING GLOBULIN; BODY-WEIGHT; ENERGY-BALANCE; FEMALE RATS; WOMEN; ESTROGEN; PREMENOPAUSAL; PROGESTERONE; DETERMINANTS; PREFERENCES AB Objective: To understand the association between ovarian hormones, non-acute satiety hormones and craving calorie dense foods in the luteal phase. Methods: 17 premenopausal women, mean age 23.2 y, mean BMI 22.4 kg/m(2) with regular menstrual cycles were studied during late follicular (FP) and luteal phases (LP). Estradiol, progesterone, DHEAS, SHBG, insulin and leptin, were measured in fasting samples. The validated' Food Craving Inventory was used to record the types of foods volunteers habitually ate - rich in fat, carbohydrate or sweet taste, as well as craved during the LP of their menstrual cycle. Results: Estradiol was inversely associated with leptin in FP (r = 0.62, p = 0.01). Leptin was inversely associated with habitual intake of sweet foods, in both phases (FP: r = 0.64, p = 0.01; LP: r = 0.63, p = 0.01). SHBG in LP was positively associated with craving sweet and carbohydrate rich foods. Hierarchical cluster analysis revealed two groups of women, one with high estradiol, high estradiol/leptin ratio, high sweet and carbohydrate cravings (p < 0.05); the other group had lower estradiol, lower estradiol/leptin ratio, and reported less craving. Conclusions: The estradiol-leptin axis may be a determinant of luteal phase craving and habitual food intake in menstruating women. Published by Elsevier Inc. C1 [Krishnan, Sridevi; Tryon, Rebecca R.; Welch, Lucas; Keim, Nancy L.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Horn, William F.; Keim, Nancy L.] ARS, USDA, Western Human Nutr Res Ctr, Obes & Metab Res Unit, 430 West Hlth Sci Dr, Davis, CA 95616 USA. RP Keim, NL (reprint author), ARS, USDA, Western Human Nutr Res Ctr, 430 West Hlth Sci Dr, Davis, CA 95616 USA. FU USDA Project [5306-51530-019-00D, 2032-51530-022-00D]; UC Davis FX This study was funded by USDA Project 5306-51530-019-00D, 2032-51530-022-00D, and Jastro Shields award from UC Davis. The U.S. Department of Agriculture is an equal opportunity provider and employer. NR 42 TC 0 Z9 0 U1 7 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0031-9384 J9 PHYSIOL BEHAV JI Physiol. Behav. PD OCT 15 PY 2016 VL 165 BP 304 EP 312 DI 10.1016/j.physbeh.2016.08.010 PG 9 WC Psychology, Biological; Behavioral Sciences SC Psychology; Behavioral Sciences GA DY0LS UT WOS:000384788900039 PM 27527001 ER PT J AU McRoberts, RE Chen, Q Domke, GM Stahl, G Saarela, S Westfall, JA AF McRoberts, Ronald E. Chen, Qi Domke, Grant M. Stahl, Goran Saarela, Svetlana Westfall, James A. TI Hybrid estimators for mean aboveground carbon per unit area SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Allometric model; Uncertainty analysis; Wood density; Carbon content ID NATIONAL FOREST INVENTORY; BIOMASS EXPANSION FACTORS; LIDAR-ASSISTED ESTIMATION; INDIVIDUAL-TREE VOLUME; WOOD DENSITY; FIELD-MEASUREMENTS; MEASUREMENT ERRORS; MODEL PREDICTIONS; TROPICAL FORESTS; HEDMARK COUNTY AB Carbon accounting is at the heart of efforts to mitigate the effects of climate change. One approach for estimating population parameters for live tree stem carbon entails three primary steps: (1) construction of an individual tree, allometric carbon model, (2) application of the model to tree-level data for a probability sample of plots, and (3) use of a probability-based (design-based) estimator of mean carbon per unit area for a population of interest. Compliance with the IPCC good practice guidance requires satisfaction of two criteria, one related to minimizing bias and one related to minimizing uncertainty. For this carbon estimation procedure, the portion of uncertainty attributed to the variance of the probability based estimator of the population mean using the plot-level predictions is usually correctly estimated, but the portion attributed to the variance of the allometric model estimator is usually ignored. The result is that the total variance of the population mean estimator cannot be asserted to comply with the IPCC good practice criteria because not only is it not minimized, it is not even correctly estimated. Within the framework of what is coming to be characterized as hybrid inference, model-based inferential methods were used to estimate the variance of the tree-level allometric model estimator which was then propagated through to the variance of the probability-based estimator of mean carbon per unit area. This combined estimator, consisting of a model-based estimator used to predict a variable for a probability sample of a population followed by a probability-estimator of the population total or mean using the sample predictions, is characterized as a hybrid estimator. For this study, two probability-based estimators of the mean were considered, simple random sampling estimators and model-assisted regression estimators that used airborne laser scanning (ALS) data as auxiliary information. The variance of the allometric model estimator incorporated variances of distributions of diameter and height measurement errors, covariances of model parameter estimators, model residual variance, and variances of distributions of wood densities and carbon content proportions. The novel features of the study included the hybrid inferential framework, consideration of six sources of uncertainty including the variances of distributions of wood densities and carbon content proportions, use of ALS data with model-assisted regression estimators of the population mean, and use of confidence intervals for the population mean as the basis for comparisons rather than intermediate products such as model prediction accuracy. The primary conclusions were that the variance of the allometric model estimator was negligible or marginally negligible relative to the variance of the probability estimator when using species-specific allometric models and simple random sampling estimators, but non-negligible when using species-specific models and model-assisted regression estimators and when using a nonspecific model with either estimator. Published by Elsevier B.V. C1 [McRoberts, Ronald E.; Domke, Grant M.] US Forest Serv, Northern Res Stn, St Paul, MN USA. [Chen, Qi] Univ Hawaii Manoa, Dept Geog, Honolulu, HI 96822 USA. [Stahl, Goran; Saarela, Svetlana] Swedish Univ Agr Sci, Umea, Sweden. [Westfall, James A.] US Forest Serv, Northern Res Stn, Newtown Sq, PA USA. RP McRoberts, RE (reprint author), US Forest Serv, Northern Res Stn, St Paul, MN USA. EM rmcroberts@fs.fed.us OI Saarela, Svetlana/0000-0002-9044-7249; Domke, Grant/0000-0003-0485-0355 NR 57 TC 2 Z9 2 U1 11 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD OCT 15 PY 2016 VL 378 BP 44 EP 56 DI 10.1016/j.foreco.2016.07.007 PG 13 WC Forestry SC Forestry GA DX5AS UT WOS:000384392800005 ER PT J AU Cowan, AD Smith, JE Fitzgerald, SA AF Cowan, Ariel D. Smith, Jane E. Fitzgerald, Stephen A. TI Recovering lost ground: Effects of soil burn intensity on nutrients and ectomycorrhiza communities of ponderosa pine seedlings SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Ectomycorrhizal fungi; Fire; Soil nutrients; Ponderosa pine; Large downed wood; Burn severity ID FINE-SCALE DISTRIBUTION; COARSE WOODY DEBRIS; MAZAMA CRATER LAKE; FUNGAL COMMUNITIES; PRESCRIBED-FIRE; CENTRAL OREGON; FOREST SOILS; ENHANCED SPECIFICITY; RESISTANT PROPAGULES; MURICATA SEEDLINGS AB Fuel accumulation and climate shifts are predicted to increase the frequency of high-severity fires in ponderosa pine (Pinus ponderosa) forests of central Oregon. The combustion of fuels containing large downed wood can result in intense soil heating, alteration of soil properties, and mortality of microbes. Previous studies show ectomycorrhizal fungi (EMF) improve ponderosa seedling establishment after fire but did not compare EMF communities at different levels of soil burn intensity in a field setting. For this study, soil burn intensity effects on nutrients and EMF communities were compared at Pringle Falls Experimental Forest, La Pine, Oregon. Twelve replicate sites were used, each with three treatments: high intensity soil burn from large downed wood combustion (HB), low intensity soil burn (LB), and unburned control (UB). Temperatures lethal to fungi were detected at 0-cm, 5-cm, and 10-cm depths in HB soils and 0-cm depth in LB soils. Ponderosa pine seedlings planted post-burn were harvested after four months for EMF root tip analysis. We found: (a) greater differences in soil properties and nutrients in HB soils compared to LB and UB soils; (b) no differences in EMF richness and diversity among treatments; (c) weak differences in community composition based on relative abundance between UB and either burn treatments; and (d) EMF composition in HB and LB treatments correlated with soil carbon and organic matter contents. These results support the hypothesis that the combustion of large downed wood can alter the soil environment directly beneath it. However, an EMF community similar to LB soils recolonized HB soils within one growing season. Community results from both burn treatments suggest an increase in patchy spatial distribution of EMF. We hypothesize that quick initiation of EMF recolonization is possible depending on the size of high intensity burn patches, proximity of low and unburned soil, and survival of nearby hosts. The importance of incorporating mixed fire effects in fuel management practices will help to provide EMF refugia for ponderosa pine forest regeneration. (C) Published by Elsevier B.V. C1 [Cowan, Ariel D.] Oregon State Univ, Coll Forestry, Dept Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA. [Smith, Jane E.] US Forest Serv, USDA, Pacific Northwest Res Stn, Forestry Sci Lab, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. [Fitzgerald, Stephen A.] Oregon State Univ, Dept Forest Engn Resources & Management, Coll Forestry, 280 Peavy Hall, Corvallis, OR 97331 USA. RP Smith, JE (reprint author), US Forest Serv, USDA, Pacific Northwest Res Stn, Forestry Sci Lab, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM jsmith01@fed.fs.us FU USDA Forest Service Pacific Northwest Research Station FX The authors would like to thank Daniel Luoma, Carol Shestak, Debbie Dumroese, Joanne Tirocke, Dan Mikowski, Loretta Ellenburg, Brett Morrissette, Mike J. Vernon, Tara Jennings, Doni McKay, Joyce Eberhart, and Rich Cronn for their assistance in methodology, training, and site setup. Thank you to Alex Enna, the Bend and Redmond fire crews for making the prescribed burn possible. Also, many thanks to Lisa Ganio, Ariel Muldoon, and Greg Brenner for their statistical advice. We greatly appreciate Lucas J. Longway, Ben Hart, Maria O. Garcia, Joseph Cagle, and Elizabeth Bowman for their help with data collection and moral support. Thank you to Chris Dunn and Melanie Jones for offering experienced advice. Additionally, we would like to thank Jeff Hatten, Daniel Luoma, Tom Horton, and anonymous reviewers for reviewing this manuscript. Lastly, we are grateful for the funding support provided by the USDA Forest Service Pacific Northwest Research Station. Mention of trade or company names does not constitute an endorsement by the US Department of Agriculture. NR 108 TC 1 Z9 1 U1 38 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD OCT 15 PY 2016 VL 378 BP 160 EP 172 DI 10.1016/j.foreco.2016.07.030 PG 13 WC Forestry SC Forestry GA DX5AS UT WOS:000384392800015 ER PT J AU Ritchie, MW AF Ritchie, Martin W. TI Multi-scale reference conditions in an interior pine-dominated landscape in northeastern California SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Stand density; Biomass; Crown area; Ponderosa pine ID FOREST STRUCTURE; RESTORATION ECOLOGY; SOUTHERN CASCADES; SPATIAL-PATTERNS; PONDEROSA; USA; FIRE; VARIABILITY; STANDS AB A plot-based census was conducted of trees >8.9 cm in breast height diameter in a 4000 ha forest in northeastern California in 1933 and 1934, prior to any harvest activity. The trees were tallied by size class and species on contiguous plots specified to be 1.01 ha in size, although some plots had a forested area less than this specification due to natural openings in the forest. In general, variability in all metrics declined as scale increased across a range from 1 ha to 244 ha, although much of this variability was in the tails; the inner quartile range appeared to be more stable, particularly so for crown area. Although metrics were derived at a smaller scale (<1 ha) from partial plots, these may be unreliable due to the study design and possible confounding factors. The landscape distribution of tree sizes was bimodal and skew positive with a maximum tree size of 189.2 cm. However locally the distribution of tree sizes was more ragged and variable in shape. Species distribution appears to have shifted in a direction away from pine dominance in the years since the census was conducted. Historically, these stands were approximately 86 percent pine by basal area, with some variation depending on slope position. The stem density was dominated by young, thrifty-mature trees of good or moderate vigor but basal area and crown area was dominated by mature or over-mature trees with moderate or poor vigor. Crown area tended to be low with 90 percent of observations between 13 and 35 percent at the 1 ha scale. Published by Elsevier B.V. C1 [Ritchie, Martin W.] US Forest Serv, Pacific Southwest Res Stn, 3644 Avtech Pkwy, Redding, CA 96002 USA. RP Ritchie, MW (reprint author), US Forest Serv, Pacific Southwest Res Stn, 3644 Avtech Pkwy, Redding, CA 96002 USA. EM mritchie@fs.fed.us FU U.S. Forest Service, Pacific Southwest Research Station FX This work was funded internally by the U.S. Forest Service, Pacific Southwest Research Station. NR 39 TC 0 Z9 0 U1 10 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD OCT 15 PY 2016 VL 378 BP 233 EP 243 DI 10.1016/j.foreco.2016.07.017 PG 11 WC Forestry SC Forestry GA DX5AS UT WOS:000384392800022 ER PT J AU Schlink, SN Lager, KM Brockmeier, SL Loving, CL Miller, LC Vorwald, AC Yang, HC Kehrli, ME Faaberg, KS AF Schlink, Sarah N. Lager, Kelly M. Brockmeier, Susan L. Loving, Crystal L. Miller, Laura C. Vorwald, Ann C. Yang, Han-Chun Kehrli, Marcus E., Jr. Faaberg, Kay S. TI Enhancement of innate immunity with granulocyte colony-stimulating factor did not mitigate disease in pigs infected with a highly pathogenic Chinese PRRSV strain SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article DE HP-PRRSV; Neutrophils; G-CSF; Swine ID RESPIRATORY SYNDROME VIRUS; SINGLE-ADMINISTRATION PEGFILGRASTIM; UNITED-STATES; MYCOPLASMA-HYOPNEUMONIAE; DAILY FILGRASTIM; DAIRY-COWS; G-CSF; EMERGENCE; NEUTROPHILS; BOVINE AB Porcine reproductive and respiratory syndrome virus (PRRSV) is responsible for one of the most economically important diseases in swine worldwide. It causes reproductive failure in sows and pneumonia in pigs that predisposes them to secondary bacterial infections. Methods to control PRRSV and/or limit secondary bacterial infections are desired to reduce the impact of this virus on animal health. Neutrophils play a major role in combatting infection; they can act as phagocytes as well as produce and release lytic enzymes that have potent antimicrobial effects leading to the destruction and clearance of bacterial pathogens. Granulocyte-colony stimulating factor (G-CSF) is a cytokine that controls the production, differentiation and function of granulocytes (including neutrophils) from the bone marrow. Recent work from our laboratory has shown that encoding porcine G-CSF in a replication-defective adenovirus (Ad5-G-CSF) and delivering a single dose to pigs induced a neutrophilia lasting more than two weeks. As secondary bacterial infection is a common occurrence following PRRSV infection, particularly following challenge with highly pathogenic (HP)-PRRSV, the aim of the current study was to evaluate the effectiveness of a single prophylactic dose of adenovirus-encoded G-CSF to mitigate secondary bacterial disease associated with HP-PRRSV infection. Administration of Ad5-G-CSF induced a significant neutrophilia as expected. However, between 1 and 2 days following HP-PRRSV challenge the number of circulating neutrophils decreased dramatically in the HP-PRRSV infected group, but not the non-infected Ad5-G-CSF group. Ad5-G-CSF administration induced monocytosis as well, which was also reduced by HP-PRRSV challenge. There was no difference in the progression of disease between the Ad5-G-CSF and Ad5-empty groups following HP-PRRSV challenge, with pneumonia and systemic bacterial infection occurring in both treatment groups. Given the impact of HP-PRRSV infection on the neutrophilia induced by the Ad5-G-CSF administration, additional studies are warranted to evaluate the timing of Ad5-G-CSF induced neutrophilia and multiple G-CSF inoculations on protection against secondary bacterial infection following PRRSV infection. Nevertheless, this study may provide insight into the pathogenesis of HP-PRRSV. Published by Elsevier B.V. C1 [Schlink, Sarah N.; Lager, Kelly M.; Brockmeier, Susan L.; Loving, Crystal L.; Miller, Laura C.; Vorwald, Ann C.; Kehrli, Marcus E., Jr.; Faaberg, Kay S.] ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, USDA, Mailstop 2S-209,1920 Dayton Ave, Ames, IA 50010 USA. [Yang, Han-Chun] China Agr Univ, Beijing, Peoples R China. [Schlink, Sarah N.] Sinclair Res Ctr LLC, Columbia, MO USA. RP Lager, KM (reprint author), ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, USDA, Mailstop 2S-209,1920 Dayton Ave, Ames, IA 50010 USA. EM kelly.lager@ars.usda.gov OI Miller, Laura/0000-0002-8946-9416 NR 37 TC 0 Z9 0 U1 11 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 EI 1873-2534 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD OCT 15 PY 2016 VL 179 BP 70 EP 76 DI 10.1016/j.vetimm.2016.08.007 PG 7 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA DV9YO UT WOS:000383298300010 PM 27590428 ER PT J AU Hsu, YM Bytnerowicz, A Fenn, ME Percy, KE AF Hsu, Yu-Mei Bytnerowicz, Andrzej Fenn, Mark E. Percy, Kevin E. TI Atmospheric dry deposition of sulfur and nitrogen in the Athabasca Oil Sands Region, Alberta, Canada SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Passive samplers; Atmospheric dry deposition; Nitrogen; Sulfur; Multi-layer inference model; Throughfall measurement ID PASSIVE SAMPLING SYSTEM; AMBIENT NITRIC-ACID; CANOPY INTERACTIONS; MULTILAYER MODEL; FORESTS; AMMONIA; AIR; ECOSYSTEMS; POLLUTANTS; PARTICLES AB Due to the potential ecological effects on terrestrial and aquatic ecosystems from atmospheric deposition in the Athabasca Oil Sands Region (AOSR), Alberta, Canada, this study was implemented to estimate atmospheric nitrogen (N) and sulfur (S) inputs. Passive samplers were used to measure ambient concentrations of ammonia (NH3), nitrogen dioxide (NO2), nitric acid,Mitrous acid (HNO3/HONO), and sulfur dioxide (SO2) in the AOSR. Concentrations of NO2 and SO2 in winter were higher than those in summer, while seasonal differences of NH3 and HNO3, HONO showed an opposite trend, with higher values in summer. Concentrations of NH3, NO2 and SO2 were high close to the emission sources (oil sands operations and urban areas). NH3 concentrations were also elevated in the southern portion of the domain indicating possible agricultural and urban emission sources to the southwest. HNO3, an oxidation endpoint, showed wider ranges of concentrations and a larger spatial extent. Concentrations of NH3, NO2, HNO3/HONO and SO2 from passive measurements and their monthly deposition velocities calculated by a multi-layer inference model (MLM) were used to calculate dry deposition of N and S. NH3 contributed the largest fraction of deposited N across the network, ranging between 0.70-1.25 kg N ha(-1) yr(-1), HNO3/HONO deposition ranged between 0.30-0.90 kg N ha(-1) yr(-1), and NO2 deposition between 0.03-0.70 kg N ha(-1) yr(-1). During the modeled period, average dry deposition of the inorganic gaseous N species ranged between 1.03 and 2.85 kg N ha(-1) yr(-1) and SO4-S deposition ranged between 026 and 2.04 kg ha(-1) yr(-1). Comparisons with co measured ion exchange resin throughfall data (8.51 kg S ha(-1) yr(-1)) indicate that modeled dry deposition combined with measured wet deposition (137 kg S ha(-1) yr(-1)) underestimated S deposition. Gas phase NH3 (71%) and HNO3 plus NO2 (79%) dry deposition fluxes dominated the total deposition of NH4-N and NO3-N, respectively. (C) 2016 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). C1 [Hsu, Yu-Mei; Percy, Kevin E.] Wood Buffalo Environm Assoc, 100-330 Thickwood Blvd, Ft McMurray, AB T9K 1Y1, Canada. [Bytnerowicz, Andrzej; Fenn, Mark E.] US Forest Serv, USDA, Pacific Southwest Res Stn, 4955 Canyon Crest Dr, Riverside, CA 92507 USA. RP Hsu, YM (reprint author), Wood Buffalo Environm Assoc, 100-330 Thickwood Blvd, Ft McMurray, AB T9K 1Y1, Canada. EM yhsu@wbea.org FU WBEA FX The authors thank WBEA for providing funding in support of this work. The content and opinions expressed by the authors in this study do not necessarily reflect the views of the WBEA or of the WBEA membership. The authors would also like to thank Kristofer Siriunas for creating isopleth maps and Thomas Clair, David Spink, Sunny Cho, and Carla Davidson for their valuable comments and suggestions. NR 46 TC 2 Z9 2 U1 37 U2 37 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD OCT 15 PY 2016 VL 568 BP 285 EP 295 DI 10.1016/j.scitotenv.2016.05.205 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA DU5NK UT WOS:000382258300033 PM 27295600 ER PT J AU Sulman, BN Roman, DT Scanlon, TM Wang, LX Novick, KA AF Sulman, Benjamin N. Roman, D. Tyler Scanlon, Todd M. Wang, Lixin Novick, Kimberly A. TI Comparing methods for partitioning a decade of carbon dioxide and water vapor fluxes in a temperate forest SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE CO2 flux; Ecohydrology; Eddy covariance; Evapotranspiration; Flux partitioning; Water use efficiency ID NET ECOSYSTEM EXCHANGE; EDDY-COVARIANCE MEASUREMENTS; NORTHERN HARDWOOD FOREST; DECIDUOUS FOREST; USE EFFICIENCY; ENERGY FLUXES; UNITED-STATES; GAS-EXCHANGE; SAP FLOW; CO2 AB The eddy covariance (EC) method is routinely used to measure net ecosystem fluxes of carbon dioxide (CO2) and evapotranspiration (ET) in terrestrial ecosystems. It is often desirable to partition CO2 flux into gross primary production (GPP) and ecosystem respiration (RE), and to partition ET into evaporation and transpiration. We applied multiple partitioning methods, including the recently-developed flux variance similarity (FVS) partitioning method, to a ten-year record of ET and CO2 fluxes measured using EC at Morgan Monroe State Forest, a temperate, deciduous forest located in south-central Indiana, USA. While the FVS method has previously been demonstrated in croplands and grasslands, this is the first evaluation of the method in a forest. CO2 fluxes were partitioned using nonlinear regressions, FVS, and sub-canopy EC measurements. ET was partitioned using FVS and sub-canopy EC measurements, and sub-canopy potential evapotranspiration was calculated as an additional constraint on forest floor evaporation. Leaf gas exchange measurements were used to parameterize a model of water use efficiency (WUE) necessary for the FVS method. Scaled leaf gas exchange measurements also provided additional independent estimates of GPP and transpiration. There was good agreement among partitioning methods for transpiration and GPP, which also agreed well with scaled leaf gas exchange measurements. There was higher variability among methods for RE and evaporation. The sub-canopy flux method yielded lower estimates of evaporation and RE than FVS and lower estimates of RE than the nonlinear regression method, likely due to the exclusion of flux sources within the canopy but above the top of the sub-canopy tower for the sub-canopy flux method. Based on a sensitivity test, FVS flux partitioning was moderately sensitive to errors in WUE values, and underestimates of WUE significantly reduced the rate at which the algorithm was able to produce a physically valid solution. FVS partitioning has unique potential for retroactive ET partitioning at EC sites, because it relies on the same continuous measurements as EC and does not require additional specialized equipment. FVS also has advantages for partitioning CO2 fluxes, since it does not rely on the mechanistic assumptions necessary for the commonly used nonlinear regression technique. (C) 2016 Elsevier B.V. All rights reserved. C1 [Sulman, Benjamin N.; Roman, D. Tyler; Novick, Kimberly A.] Indiana Univ, Sch Publ & Environm Affairs, 702 N Walnut Grove Ave, Bloomington, IN 47405 USA. [Sulman, Benjamin N.] Indiana Univ, Dept Biol, 1001 E 3rd St, Bloomington, IN 47405 USA. [Sulman, Benjamin N.] Princeton Univ, Dept Geosci, Program Atmospher & Ocean Sci, 300 Forrestal Rd, Princeton, NJ 08544 USA. [Roman, D. Tyler] Forest Serv, USDA, Northern Res Stn, 1831 Hwy 169 E, Grand Rapids, MN 55744 USA. [Scanlon, Todd M.] Univ Virginia, Dept Environm Sci, 291 McCormick Rd, Charlottesville, VA 22904 USA. [Wang, Lixin] IUPUI, Dept Earth Sci, 723 W Michigan St, Indianapolis, IN 46202 USA. RP Sulman, BN (reprint author), Princeton Univ, Dept Geosci, Program Atmospher & Ocean Sci, 300 Forrestal Rd, Princeton, NJ 08544 USA. EM bsulman@princeton.edu FU Indiana University Collaborative Research Grant Program; Ameriflux Management Project; US Department of Energy FX Special thanks to R.P. Phillips, E.R. Brzostek, S. Scott, D. Dragoni, H.P. Schmid, and S. Grimmond for contributions to the long data record at the site. This work was funded by the Indiana University Collaborative Research Grant Program and the Ameriflux Management Project (managed by Lawrence Berkeley National Laboratory with support from the US Department of Energy). Additional computing support was provided by the Indiana University Geography Department. Thanks to the two anonymous reviewers for their helpful comments and suggestions. NR 54 TC 2 Z9 2 U1 21 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD OCT 15 PY 2016 VL 226 BP 229 EP 245 DI 10.1016/j.agrformet.2016.06.002 PG 17 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA DS2KK UT WOS:000380598200019 ER PT J AU Lycett, SJ Bodewes, R Pohlmann, A Banks, J Banyai, K Boni, MF Bouwstra, R Breed, AC Brown, IH Chen, HL Dan, A DeLiberto, TJ Diep, N Gilbert, M Hill, S Ip, HS Ke, CW Kida, H Killian, ML Koopmans, MP Kwon, JH Lee, DH Lee, YJ Lu, L Monne, I Pasick, J Pybus, OG Rambaut, A Robinson, TP Sakoda, Y Zohari, S Song, CS Swayne, DE Torchetti, MK Tsai, HJ Fouchier, RAM Beer, M Woolhouse, M Kuiken, T AF Lycett, Samantha J. Bodewes, Rogier Pohlmann, Anne Banks, Jill Banyai, Krisztian Boni, Maciej F. Bouwstra, Ruth Breed, Andrew C. Brown, Ian H. Chen, Hualan Dan, Adam DeLiberto, Thomas J. Diep, Nguyen Gilbert, Marius Hill, Sarah Ip, Hon S. Ke, Chang Wen Kida, Hiroshi Killian, Mary Lea Koopmans, Marion P. Kwon, Jung-Hoon Lee, Dong-Hun Lee, Youn Jeong Lu, Lu Monne, Isabella Pasick, John Pybus, Oliver G. Rambaut, Andrew Robinson, Timothy P. Sakoda, Yoshihiro Zohari, Siamak Song, Chang-Seon Swayne, David E. Torchetti, Mia Kim Tsai, Hsiang-Jung Fouchier, Ron A. M. Beer, Martin Woolhouse, Mark Kuiken, Thijs CA Global Consortium H5N8 Related TI Role for migratory wild birds in the global spread of avian influenza H5N8 SO SCIENCE LA English DT Article ID VIRUSES AB Avian influenza viruses affect both poultry production and public health. A subtype H5N8 (clade 2.3.4.4) virus, following an outbreak in poultry in South Korea in January 2014, rapidly spread worldwide in 2014-2015. Our analysis of H5N8 viral sequences, epidemiological investigations, waterfowl migration, and poultry trade showed that long-distance migratory birds can play a major role in the global spread of avian influenza viruses. Further, we found that the hemagglutinin of clade 2.3.4.4 virus was remarkably promiscuous, creating reassortants with multiple neuraminidase subtypes. Improving our understanding of the circumpolar circulation of avian influenza viruses in migratory waterfowl will help to provide early warning of threats from avian influenza to poultry, and potentially human, health. C1 [Lycett, Samantha J.] Univ Edinburgh, Roslin Inst, Roslin EH25 9RG, Midlothian, Scotland. [Bodewes, Rogier] Univ Utrecht, Fac Vet Med, Dept Farm Anim Hlth, NL-3584 CL Utrecht, Netherlands. [Pohlmann, Anne; Beer, Martin] Friedrich Loeffler Inst, Inst Diagnost Virol, D-17493 Greifswald, Germany. [Banks, Jill; Brown, Ian H.] Anim & Plant Hlth Agcy, Virol Dept, Woodham Lane, Addlestone KT15 3NB, Surrey, England. [Banyai, Krisztian] Hungarian Acad Sci, Agr Res Ctr, Inst Vet Med Res, H-1143 Budapest, Hungary. [Boni, Maciej F.] Univ Oxford, Nuffield Dept Med, Ctr Trop Med, Oxford OX3 7FZ, England. [Boni, Maciej F.; Diep, Nguyen] Univ Oxford, Clin Res Unit, Wellcome Trust Major Overseas Programme, Ho Chi Minh City, Vietnam. [Bouwstra, Ruth] Wageningen Univ & Res Ctr, Cent Vet Inst, Dept Virol, NL-8221 RA Lelystad, Netherlands. [Bouwstra, Ruth] Anim Hlth Serv, NL-7400 AA Deventer, Netherlands. [Breed, Andrew C.] Anim & Plant Hlth Agcy, Dept Epidemiol Sci, Woodham Lane, Addlestone KT15 3NB, Surrey, England. [Chen, Hualan] Chinese Acad Agr Sci, Harbin Vet Res Inst, Harbin 150001, Peoples R China. [Dan, Adam] Natl Food Chain Safety Off, Vet Diagnost Directorate, H-1149 Budapest, Hungary. [DeLiberto, Thomas J.] Wildlife Serv, Natl Wildlife Res Ctr, USDA, Ft Collins, CO 80521 USA. [Gilbert, Marius] Univ Libre Bruxelles, Spatial Epidemiol Lab SpELL, B-1050 Brussels, Belgium. [Gilbert, Marius] Fonds Natl Rech Sci, B-1000 Brussels, Belgium. [Hill, Sarah; Pybus, Oliver G.] Univ Oxford, Dept Zool, Oxford OX1 3PS, England. [Ip, Hon S.] US Geol Survey, Wildlife Dis Diagnost Labs Branch, Natl Wildlife Hlth Ctr, Madison, WI 53711 USA. [Ke, Chang Wen] Ctr Dis Control & Prevent Guangdong Prov, Inst Microbiol, Guangzhou 511430, Guangdong, Peoples R China. [Kida, Hiroshi] Hokkaido Univ, Res Ctr Zoonosis Control, Sapporo, Hokkaido 0010020, Japan. [Killian, Mary Lea; Torchetti, Mia Kim] Vet Serv, Natl Vet Serv Labs, USDA, Ames, IA 50010 USA. [Koopmans, Marion P.; Fouchier, Ron A. M.; Kuiken, Thijs] Erasmus Univ, Dept Virosci, Med Ctr, NL-3015 CN Rotterdam, Netherlands. [Kwon, Jung-Hoon; Song, Chang-Seon] Konkuk Univ, Coll Vet Med, Avian Dis Lab, Seoul 143701, South Korea. [Lee, Dong-Hun; Swayne, David E.] USDA, Southeast Poultry Res Lab, Athens, GA 30605 USA. [Lee, Youn Jeong] Anim & Plant Quarantine Agcy, Avian Dis Div, Gimcheon, South Korea. [Lu, Lu; Rambaut, Andrew; Woolhouse, Mark] Univ Edinburgh, Ctr Immun Infect & Evolut, Edinburgh EH9 3FL, Midlothian, Scotland. [Monne, Isabella] Ist Zooprofilatt Sperimentale Venezie, Res & Innovat Dept, I-1035020 Padua, Italy. [Pasick, John] Canadian Food Inspect Agcy, Natl Ctr Foreign Anim Dis, Winnipeg, MB R3E 3M4, Canada. [Pasick, John] Canadian Food Inspect Agcy, Guelph, ON N1G 4S9, Canada. [Robinson, Timothy P.] Int Livestock Res Inst ILRI, Livestock Syst & Environm LSE, POB 30709, Nairobi 00100, Kenya. [Sakoda, Yoshihiro] Hokkaido Univ, Grad Sch Vet Med, Sapporo, Hokkaido 0600818, Japan. [Zohari, Siamak] Natl Vet Inst, Dept Virol Immunobiol & Parasitol, SE-75189 Uppsala, Sweden. [Tsai, Hsiang-Jung] Council Agr, Anim Hlth Res Inst, New Taipei 25158, Taiwan. RP Kuiken, T (reprint author), Erasmus Univ, Dept Virosci, Med Ctr, NL-3015 CN Rotterdam, Netherlands. EM t.kuiken@erasmusmc.nl RI Brown, Ian/E-1119-2011; Dan, Adam/G-6018-2012; OI Dan, Adam/0000-0001-7849-7721; Hill, Sarah/0000-0002-2995-2596; Lycett, Samantha/0000-0003-3159-596X; Banyai, Krisztian/0000-0002-6270-1772; Zohari, Siamak/0000-0002-0017-4233 FU European Commission [643476]; European Commission FP7 program [278433]; U.S. Geological Survey Ecosystems Mission Area; National Institutes of Health [1R01AI101028-02A1]; United Kingdom Research Council Environmental and Social Ecology of Human Infectious Diseases UrbanZoo program [G1100783/1]; Biotechnology and Biological Sciences Research Council (BBSRC) Zoonoses in Livestock in Kenya ZooLinK programs [BB/L019019/1]; CGIAR Research Programme on Agriculture for Nutrition and Health (A4NH); Canadian Food Inspection Agency; Hungarian Academy of Sciences Lendulet (Momentum) program; Wellcome Trust [093724/B/10/Z]; University of Edinburgh Chancellor's Fellowship scheme; Roslin Institute BBSRC strategic program [BB/J004227/1]; Centre of Expertise in Animal Disease Outbreaks (EPIC) FX This study was financially supported by the European Commission H2020 program under contract number 643476 (www.compare-europe.eu) (to A.P., J.B., A.B., I.B., M.P.K., A.R., R.A.M.F., M.B., M.W., and T.K.), European Commission FP7 program under contract number 278433 (PREDEMICS) (to A.R.), the U.S. Geological Survey Ecosystems Mission Area (to H.S.I.), National Institutes of Health grant number 1R01AI101028-02A1 (to M.G.), United Kingdom Research Council Environmental and Social Ecology of Human Infectious Diseases UrbanZoo program (G1100783/1), Biotechnology and Biological Sciences Research Council (BBSRC) Zoonoses in Livestock in Kenya ZooLinK (BB/L019019/1) programs (to T.P.R. and M.W.), CGIAR Research Programme on Agriculture for Nutrition and Health (A4NH) (to T.P.R.), Canadian Food Inspection Agency (to J.P.), Hungarian Academy of Sciences Lendulet (Momentum) program (to K.B.) and the Wellcome Trust (grant number 093724/B/10/Z) (to M.W. and A.R.). S.J.L. is supported by the University of Edinburgh Chancellor's Fellowship scheme, the Roslin Institute BBSRC strategic program grant (BB/J004227/1), and the Centre of Expertise in Animal Disease Outbreaks (EPIC). We gratefully acknowledge the originating laboratories, where specimens were first obtained, and the submitting laboratories, where sequence data were generated and submitted to the EpiFlu Database of the Global Initiative on Sharing All Influenza Data (GISAID), on which this research is based. All contributors of data may be contacted directly via the GISAID website (http://platform.gisaid.org). The accession numbers (GenBank, GISAID, and/or workset identification numbers) of all genetic sequences used in this study are provided in table S9 and are accessible from the website of GISAID (http://platform.gisaid.org). We acknowledge Y. Berhane and T. Hisanaga for sequencing the Canadian virus isolates and G. Koch for his technical advice on the poultry outbreaks in the Netherlands. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. Any use of trade products or firm names is for descriptive purposes and does not imply endorsement by the U.S. government. NR 13 TC 2 Z9 2 U1 22 U2 22 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 EI 1095-9203 J9 SCIENCE JI Science PD OCT 14 PY 2016 VL 354 IS 6309 BP 213 EP 217 DI 10.1126/science.aaf8852 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EC0UN UT WOS:000387816500041 ER PT J AU Labourel, A Crouch, LI Bras, JLA Jackson, A Rogowski, A Gray, J Yadav, MP Henrissat, B Fontes, CMGA Gilbert, HJ Najmudin, S Basle, A Cuskin, F AF Labourel, Aurore Crouch, Lucy I. Bras, Joana L. A. Jackson, Adam Rogowski, Artur Gray, Joseph Yadav, Madhav P. Henrissat, Bernard Fontes, Carlos M. G. A. Gilbert, Harry J. Najmudin, Shabir Basle, Arnaud Cuskin, Fiona TI The Mechanism by Which Arabinoxylanases Can Recognize Highly Decorated Xylans SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article DE cellulosome; crystallography; enzyme kinetics; enzyme mechanism; glycoside hydrolase ID CARBOHYDRATE-BINDING MODULES; CLOSTRIDIUM-THERMOCELLUM; POLYSACCHARIDE RECOGNITION; SUBSTRATE-SPECIFICITY; CRYSTAL-STRUCTURES; DEGRADATION; DECONSTRUCTION; CELLULOSOMES; BIOCHEMISTRY; REFINEMENT AB The enzymatic degradation of plant cell walls is an important biological process of increasing environmental and industrial significance. Xylan, a major component of the plant cell wall, consists of a backbone of -1,4-xylose (Xylp) units that are often decorated with arabinofuranose (Araf) side chains. A large penta-modular enzyme, CtXyl5A, was shown previously to specifically target arabinoxylans. The mechanism of substrate recognition displayed by the enzyme, however, remains unclear. Here we report the crystal structure of the arabinoxylanase and the enzyme in complex with ligands. The data showed that four of the protein modules adopt a rigid structure, which stabilizes the catalytic domain. The C-terminal non-catalytic carbohydrate binding module could not be observed in the crystal structure, suggesting positional flexibility. The structure of the enzyme in complex with Xylp--1,4-Xylp--1,4-Xylp-[-1,3-Araf]--1,4-Xylp showed that the Araf decoration linked O-3 to the xylose in the active site is located in the pocket (-2* subsite) that abuts onto the catalytic center. The -2* subsite can also bind to Xylp and Arap, explaining why the enzyme can utilize xylose and arabinose as specificity determinants. Alanine substitution of Glu(68), Tyr(92), or Asn(139), which interact with arabinose and xylose side chains at the -2* subsite, abrogates catalytic activity. Distal to the active site, the xylan backbone makes limited apolar contacts with the enzyme, and the hydroxyls are solvent-exposed. This explains why CtXyl5A is capable of hydrolyzing xylans that are extensively decorated and that are recalcitrant to classic endo-xylanase attack. C1 [Labourel, Aurore; Crouch, Lucy I.; Jackson, Adam; Rogowski, Artur; Gray, Joseph; Gilbert, Harry J.; Basle, Arnaud; Cuskin, Fiona] Newcastle Univ, Inst Cell & Mol Biosci, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England. [Bras, Joana L. A.; Fontes, Carlos M. G. A.; Najmudin, Shabir] Univ Lisbon, CIISA Fac Med Vet, Polo Univ Alto Ajuda, Ave Univ Tecn, P-1300477 Lisbon, Portugal. [Bras, Joana L. A.; Fontes, Carlos M. G. A.] NZYTech Genes & Enzymes, P-1649038 Lisbon, Portugal. [Yadav, Madhav P.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Henrissat, Bernard] Aix Marseille Univ, CNRS, UMR7857, Architecture & Fonct Macromol Biol, F-13288 Marseille, France. [Henrissat, Bernard] INRA, Architecture & Fonct Macromol Biol USC1408, F-13288 Marseille, France. [Henrissat, Bernard] King Abdulaziz Univ, Dept Biol Sci, Jedda 21589, Saudi Arabia. RP Basle, A; Cuskin, F (reprint author), Newcastle Univ, Inst Cell & Mol Biosci, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England.; Najmudin, S (reprint author), Univ Lisbon, CIISA Fac Med Vet, Polo Univ Alto Ajuda, Ave Univ Tecn, P-1300477 Lisbon, Portugal. EM shabir@fmv.ulosboa.pt; arnaud.basle@ncl.uk; Fiona.cuskin@newcastle.ac.uk RI CIISA, FMV/D-2242-2016; Fac Sci, KAU, Biol Sci Dept/L-4228-2013 FU European Research Council [322820]; Biotechnology and Biological Research Council [BB/K020358/1, BB/K001949/1]; Wellcome Trust [RES/0120/7613]; Agence Nationale de la Recherche [ANR 12-BIME-0006-01]; Fundacao para a Ciencia e Tecnologia [PTDC/BIAPRO/103980/2008, PTDC/BIAMIC/5947/2014] FX This work was supported in part by European Research Council Grant 322820 (to H. J. G. and B. H.), Biotechnology and Biological Research Council Grants BB/K020358/1 and BB/K001949/1 (to H. J. G.), Wellcome Trust Grant RES/0120/7613 (to H. J. G.), Agence Nationale de la Recherche Grant ANR 12-BIME-0006-01 (to B. H.), and Fundacao para a Ciencia e Tecnologia Grants PTDC/BIAPRO/103980/2008 and PTDC/BIAMIC/5947/2014 (to C. M. G. A. F.). The authors declare that they have no conflicts of interest with the contents of this article. NR 49 TC 0 Z9 0 U1 11 U2 11 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 EI 1083-351X J9 J BIOL CHEM JI J. Biol. Chem. PD OCT 14 PY 2016 VL 291 IS 42 BP 22149 EP 22159 DI 10.1074/jbc.M116.743948 PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EA1DL UT WOS:000386332600028 PM 27531750 ER PT J AU Menendez-Bravo, S Roulet, J Sabatini, M Comba, S Dunn, R Gramajo, H Arabolaza, A AF Menendez-Bravo, Simon Roulet, Julia Sabatini, Mart-N Comba, Santiago Dunn, Robert Gramajo, Hugo Arabolaza, Ana TI High cell density production of multimethyl-branched long-chain esters in Escherichia coli and determination of their physicochemical properties SO BIOTECHNOLOGY FOR BIOFUELS LA English DT Article DE Escherichia coli; Wax production; Metabolic engineering; Fed-batch fermentation; Oleochemicals ID JOJOBA OIL; HETEROLOGOUS PRODUCTION; RENEWABLE RESOURCES; VEGETABLE-OILS; BASE-STOCKS; FATTY-ACIDS; WAX ESTERS; BIOSYNTHESIS; EXPRESSION; BIOFUELS AB Background: Microbial synthesis of oleochemicals derived from native fatty acid (FA) metabolism has presented significant advances in recent years. Even so, native FA biosynthetic pathways often provide a narrow variety of usually linear hydrocarbons, thus yielding end products with limited structural diversity. To overcome this limitation, we took advantage of a polyketide synthase-based system from Mycobacterium tuberculosis and developed an Escherichia coli platform with the capacity to synthesize multimethyl-branched long-chain esters (MBE) with novel chemical structures. Results: With the aim to initiate the characterization of these novel waxy compounds, here, we describe the chassis optimization of the MBE producer E. coli strain for an up-scaled oil production. By carrying out systematic metabolic engineering, we improved the final titer to 138.1 +/- 5.3 mg MBE L-1 in batch cultures. Fed-batch microbial fermentation process was also optimized achieving a maximum yield of 790.2 +/- 6.9 mg MBE L-1 with a volumetric productivity of 15.8 +/- 1.1 mg MBE (L h)(-1). Purified MBE oil was subjected to various physicochemical analyses, including differential scanning calorimetry (DSC) and pressurized-differential scanning calorimetry (P-DSC) studies. Conclusions: The analysis of the pour point, DSC, and P-DSC data obtained showed that bacterial MBE possess improved cold flow properties than several plant oils and some chemically modified derivatives, while exhibiting high oxidation stability at elevated temperatures. These encouraging data indicate that the presence of multiple methyl branches in these novel esters, indeed, conferred favorable properties which are superior to those of linear esters. C1 [Menendez-Bravo, Simon; Roulet, Julia; Sabatini, Mart-N; Comba, Santiago; Gramajo, Hugo; Arabolaza, Ana] Univ Nacl Rosario, Fac Ciencias Bioquim & Farmaceut, Consejo Nacl Invest Cient & Tecn, Microbiol Div,IBR Inst Biol Mol & Celular Rosario, RA-2000 Rosario, Santa Fe, Argentina. [Dunn, Robert] ARS, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. RP Arabolaza, A (reprint author), Univ Nacl Rosario, Fac Ciencias Bioquim & Farmaceut, Consejo Nacl Invest Cient & Tecn, Microbiol Div,IBR Inst Biol Mol & Celular Rosario, RA-2000 Rosario, Santa Fe, Argentina. EM arabolaza@ibr-conicet.gov.ar FU Y-TEC/ANPCyT Grant [PID 2013-0042]; ANPCyT Grant [PICT 2013-1981] FX This work was supported by Y-TEC/ANPCyT Grant PID 2013-0042 to HG. PID 2013-0042 belongs to the Industrial Biotechnology Program of YPF-Tecnologia. This work was also supported by ANPCyT Grant PICT 2013-1981 to AA. NR 49 TC 0 Z9 0 U1 5 U2 5 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1754-6834 J9 BIOTECHNOL BIOFUELS JI Biotechnol. Biofuels PD OCT 14 PY 2016 VL 9 AR 215 DI 10.1186/s13068-016-0631-x PG 14 WC Biotechnology & Applied Microbiology; Energy & Fuels SC Biotechnology & Applied Microbiology; Energy & Fuels GA DZ1EA UT WOS:000385580500001 PM 27757170 ER PT J AU Brown, AMV Wasala, SK Howe, DK Peetz, AB Zasada, IA Denver, DR AF Brown, Amanda M. V. Wasala, Sulochana K. Howe, Dana K. Peetz, Amy B. Zasada, Inga A. Denver, Dee R. TI Genomic evidence for plant-parasitic nematodes as the earliest Wolbachia hosts SO SCIENTIFIC REPORTS LA English DT Article ID LATERAL GENE-TRANSFER; CYTOPLASMIC INCOMPATIBILITY; MAXIMUM-LIKELIHOOD; SOFTWARE PACKAGE; ET-AL.; ENDOSYMBIONT; EVOLUTION; BACTERIA; BIOLOGY; EXPRESSION AB Wolbachia, one of the most widespread endosymbionts, is a target for biological control of mosquito-borne diseases (malaria and dengue virus), and antibiotic elimination of infectious filarial nematodes. We sequenced and analyzed the genome of a new Wolbachia strain (wPpe) in the plant-parasitic nematode Pratylenchus penetrans. Phylogenomic analyses placed wPpe as the earliest diverging Wolbachia, suggesting two evolutionary invasions into nematodes. The next branches comprised strains in sap-feeding insects, suggesting Wolbachia may have first evolved as a nutritional mutualist. Genome size, protein content, %GC, and repetitive DNA allied wPpe with mutualistic Wolbachia, whereas gene repertoire analyses placed it between parasite (A, B) and mutualist (C, D, F) groups. Conservation of iron metabolism genes across Wolbachia suggests iron homeostasis as a potential factor in its success. This study enhances our understanding of this globally pandemic endosymbiont, highlighting genetic patterns associated with host changes. Combined with future work on this strain, these genomic data could help provide potential new targets for plant-parasitic nematode control. C1 [Brown, Amanda M. V.; Wasala, Sulochana K.; Howe, Dana K.; Denver, Dee R.] Oregon State Univ, Dept Integrat Biol, 3029 Cordley Hall, Corvallis, OR 97331 USA. [Peetz, Amy B.; Zasada, Inga A.] USDA ARS, Hort Crops Res Lab, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. RP Brown, AMV (reprint author), Oregon State Univ, Dept Integrat Biol, 3029 Cordley Hall, Corvallis, OR 97331 USA. EM browaman@oregonstate.edu FU United States Department of Agriculture grant [SCA 58-5358-3-026] FX We thank Anne-Marie Girard and Mark Dasenko at the Center for Genome Research and Bioinformatics (CGRB) at Oregon State University for assistance with confocal microscopy and sequencing. We also thank Wendy Phillips at the USDA-Agricultural Research Service for valuable discussions of this work and for assistance with nematode collection. We thank John Werren and Amanda Dolen for providing positive control Wolbachia group A + B DNA. This work was supported by the United States Department of Agriculture grant SCA 58-5358-3-026. NR 87 TC 0 Z9 0 U1 27 U2 27 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD OCT 13 PY 2016 VL 6 AR 34955 DI 10.1038/srep34955 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DY9OK UT WOS:000385463500001 PM 27734894 ER PT J AU Tingley, MW Ruiz-Gutierrez, V Wilkerson, RL Howell, CA Siegel, RB AF Tingley, Morgan W. Ruiz-Gutierrez, Viviana Wilkerson, Robert L. Howell, Christine A. Siegel, Rodney B. TI Pyrodiversity promotes avian diversity over the decade following forest fire SO PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Article DE bird diversity; post-fire ecosystems; time since fire; mixed-severity fire; hierarchical Bayesian; occupancy ID MIXED-CONIFER FORESTS; SIERRA-NEVADA; BIODIVERSITY CONSERVATION; SPECIES-DIVERSITY; ANT COMMUNITIES; SEVERITY; WILDFIRE; REGIMES; USA; MANAGEMENT AB An emerging hypothesis in fire ecology is that pyrodiversity increases species diversity. We test whether pyrodiversity-defined as the standard deviation of fire severity-increases avian biodiversity at two spatial scales, and whether and how this relationship may change in the decade following fire. We use a dynamic Bayesian community model applied to a multi-year dataset of bird surveys at 1106 points sampled across 97 fires in montane California. Our results provide strong support for a positive relationship between pyrodiversity and bird diversity. This relationship interacts with time since fire, with pyrodiversity having a greater effect on biodiversity at 10 years post-fire than at 1 year post-fire. Immediately after fires, patches of differing burn severities hold similar bird communities, but over the ensuing decade, bird assemblages within patches of contrasting severities differentiate. When evaluated at the scale of individual fires, fires with a greater heterogeneity of burn severities hold substantially more species. High spatial heterogeneity in severity, sometimes called 'mixed-severity fire', is a natural part of wildfire regimes in western North America, but may be jeopardized by climate change and a legacy of fire suppression. Forest management that encourages mixed-severity fire may be critical for sustaining biodiversity across fire-prone landscapes. C1 [Tingley, Morgan W.] Univ Connecticut, Ecol & Evolutionary Biol, 75 North Eagleville Rd,Unit 3043, Storrs, CT 06269 USA. [Tingley, Morgan W.; Wilkerson, Robert L.; Siegel, Rodney B.] Inst Bird Populat, POB 1346, Point Reyes Stn, CA 94956 USA. [Ruiz-Gutierrez, Viviana] Cornell Lab Ornithol, 159 Sapsucker Woods Rd, Ithaca, NY 14850 USA. [Howell, Christine A.] US Forest Serv, USDA, Pacific Southwest Reg, 1323 Club Dr, Vallejo, CA 94592 USA. [Howell, Christine A.] US Forest Serv, USDA, Pacific Southwest Reg Stn, 800 Buchanan St, Albany, CA 94710 USA. RP Tingley, MW (reprint author), Univ Connecticut, Ecol & Evolutionary Biol, 75 North Eagleville Rd,Unit 3043, Storrs, CT 06269 USA.; Tingley, MW (reprint author), Inst Bird Populat, POB 1346, Point Reyes Stn, CA 94956 USA. EM morgan.tingley@uconn.edu FU Pacific Southwest Region of the USDA Forest Service and Environment Now FX This project was supported financially by the Pacific Southwest Region of the USDA Forest Service and Environment Now. NR 63 TC 0 Z9 0 U1 19 U2 19 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8452 EI 1471-2954 J9 P ROY SOC B-BIOL SCI JI Proc. R. Soc. B-Biol. Sci. PD OCT 12 PY 2016 VL 283 IS 1840 AR 20161703 DI 10.1098/rspb.2016.1703 PG 9 WC Biology; Ecology; Evolutionary Biology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Evolutionary Biology GA EA3GN UT WOS:000386490000014 ER PT J AU Stephens, JL Dinger, EC Alexander, JD Mohren, SR Ralph, CJ Sarr, DA AF Stephens, Jaime L. Dinger, Eric C. Alexander, John D. Mohren, Sean R. Ralph, C. John Sarr, Daniel A. TI Bird Communities and Environmental Correlates in Southern Oregon and Northern California, USA SO PLOS ONE LA English DT Article ID KLAMATH-SISKIYOU ECOREGION; UNITED-STATES; FOREST; SCALE; CONSERVATION; BIODIVERSITY; HABITAT; PRECIPITATION; BIOGEOGRAPHY; ECOSYSTEMS AB We examined avian community ecology in the Klamath Ecoregion and determined that individual bird species co-exist spatially to form 29 statistically distinguishable bird groups. We identified climate, geography, and vegetation metrics that are correlated with these 29 bird groups at three scales: Klamath Ecoregion, vegetation formation (agriculture, conifer, mixed conifer/hardwood, shrubland), and National Park Service unit. Two climate variables (breeding season mean temperature and temperature range) and one geography variable (elevation) were correlated at all scales, suggesting that for some vegetation formations and park units there is sufficient variation in climate and geography to be an important driver of bird communities, a level of variation we expected only at the broader scale. We found vegetation to be important at all scales, with coarse metrics (environmental site potential and existing vegetation formation) meaningful across all scales and structural vegetation patterns (e.g. succession, disturbance) important only at the scale of vegetation formation or park unit. Additionally, we examined how well six National Park Service units represent bird communities in the broader Klamath Ecoregion. Park units are inclusive of most bird communities with the exception of the oak woodland community; mature conifer forests are well represented, primarily associated with conifer canopy and lacking multi-layered structure. Identifying environmental factors that shape bird communities at three scales within this region is important; such insights can inform local and regional land management decisions necessary to ensure bird conservation in this globally significant region. C1 [Stephens, Jaime L.; Alexander, John D.; Ralph, C. John] Klamath Bird Observ, Ashland, OR 97520 USA. [Dinger, Eric C.; Mohren, Sean R.; Sarr, Daniel A.] Klamath Network, Natl Pk Serv, Ashland, OR USA. [Mohren, Sean R.] Crater Lake Natl Pk, Crater Lake, OR USA. [Ralph, C. John] USDA Forest Serv, Pacific Southwest Res Stn, Redwood Sci Lab, Arcata, CA USA. RP Stephens, JL (reprint author), Klamath Bird Observ, Ashland, OR 97520 USA. EM jlh@klamathbird.org OI Stephens, Jaime/0000-0002-4929-5263 FU United States Department of Interior National Park Service [P13AC01404] FX Funding for this paper was provided to Klamath Bird Observatory by the United States Department of Interior National Park Service through Task Agreement P13AC01404. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the opinions or policies of the U.S. Government. Mention of trade names or commercial products does not constitute their endorsement by the U.S. Government. NR 59 TC 0 Z9 0 U1 5 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD OCT 12 PY 2016 VL 11 IS 10 AR e0163906 DI 10.1371/journal.pone.0163906 PG 24 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ0CQ UT WOS:000385505300033 PM 27732625 ER PT J AU Guo, DL Xi, FF Yu, YH Zhang, XY Zhang, GH Zhong, GY AF Guo, Da-Long Xi, Fei-Fei Yu, Yi-He Zhang, Xiao-Yu Zhang, Guo-Hai Zhong, Gan-Yuan TI Comparative RNA-Seq profiling of berry development between table grape 'Kyoho' and its early-ripening mutant 'Fengzao' SO BMC GENOMICS LA English DT Article DE Grape; Early ripening; RNA-Seq; Bud mutant; Kyoho ID CLASS IV CHITINASE; FRUIT; GENE; IDENTIFICATION; EXPRESSION; REVEALS; CALCIUM; SYSTEM; STRESS; ROS AB Background: Early ripening is an important desirable attribute for fruit crops. 'Kyoho' is a popular table grape cultivar in many Asian countries. 'Fengzao' is a bud mutant of 'Kyoho' and ripens nearly 30 days earlier than 'Kyoho'. To identify genes controlling early fruit development and ripening in 'Fengzao', RNA-Seq profiles of the two cultivars were compared at 8 different berry developmental stages in both berry peel and flesh tissues. Methods: RNA-Seq profiling of berry development between 'Kyoho' and 'Fenzhao' were obtained using the Illumina HiSeq system and analyzed using various statistical methods. Expression patterns of several selected genes were validated using qRT-PCR. Results: About 447 millions of RNA-Seq sequences were generated from 40 RNA libraries covering various different berry developmental stages of 'Fengzao' and 'Kyoho'. These sequences were mapped to 23,178 and 22,982 genes in the flesh and peel tissues, respectively. While most genes in 'Fengzao' and 'Kyoho' shared similar expression patterns over different berry developmental stages, there were many genes whose expression were detected only in 'Fengzao' or 'Kyoho'. We observed 10 genes in flesh tissue and 22 genes in peel tissue were differentially expressed at FDR <= 0.05 when the mean expression of 'Fengzao' and 'Kyoho' were compared. The most noticeable one was VIT_214s0030g00950 (a superoxide dismutase gene). This ROS related gene showed lower expression levels in 'Fengzao' than 'Kyoho' in both peel and flesh tissues across various berry developmental stages with the only exception at veraison. VIT_200s0238g00060 (TMV resistance protein n-like) and VIT_213s0067g01100 (disease resistance protein at3g14460-like) were the two other noticeable genes which were found differentially expressed between the two cultivars in both peel and flesh tissues. GO functional category and KEGG enrichment analysis of DEGs indicated that gene activities related to stress and ROS were altered between the two cultivars in both flesh and peel tissues. Several differentially expressed genes of interest were successfully validated using qRT-PCR. Conclusions: Comparative profiling analysis revealed a few dozens of genes which were differentially expressed in the developing berries of 'Kyoho' and its early ripening mutant 'Fengzao'. Further analysis of these differentially expressed genes suggested that gene activities related to ROS and pathogenesis were likely involved in contributing to the early ripening in 'Fengzao'. C1 [Guo, Da-Long; Xi, Fei-Fei; Yu, Yi-He; Zhang, Xiao-Yu; Zhang, Guo-Hai] Henan Univ Sci & Technol, Coll Forestry, Luoyang 471003, Henan Province, Peoples R China. [Guo, Da-Long; Zhong, Gan-Yuan] USDA ARS, Grape Genet Res Unit, Geneva, NY 14456 USA. RP Zhong, GY (reprint author), USDA ARS, Grape Genet Res Unit, Geneva, NY 14456 USA. EM ganyuan.zhong@ars.usda.gov OI Guo, Da-Long/0000-0001-5379-5255 FU Natural Science Foundation of China (NSFC) [31372026, 31672106]; Plan for Scientific Innovation Talent of Henan Province [164100510006]; Program for Science&Technology Innovation Talents in the University of Henan Province [13HASTIT004]; Innovative Research Team in Henan University of Science & Technology, China [2015TTD003] FX This work was supported by Natural Science Foundation of China (NSFC: 31372026, 31672106), Plan for Scientific Innovation Talent of Henan Province (164100510006), Program for Science&Technology Innovation Talents in the University of Henan Province (13HASTIT004) and Innovative Research Team in Henan University of Science & Technology (2015TTD003), China. The RNA-Seq work was carried out at the USDA-Agricultural Research Service (ARS), Grape Genetics research Unit, Geneva, New York, USA. NR 59 TC 0 Z9 0 U1 8 U2 8 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD OCT 12 PY 2016 VL 17 AR 795 DI 10.1186/s12864-016-3051-1 PG 17 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DZ0EN UT WOS:000385510400003 PM 27729006 ER PT J AU Leisso, RS Gapper, NE Mattheis, JP Sullivan, NL Watkins, CB Giovannoni, JJ Schaffer, RJ Johnston, JW Hanrahan, I Hertog, MLATM Nicolai, BM Rudell, DR AF Leisso, Rachel S. Gapper, Nigel E. Mattheis, James P. Sullivan, Nathanael L. Watkins, Christopher B. Giovannoni, James J. Schaffer, Robert J. Johnston, Jason W. Hanrahan, Ines Hertog, Maarten L. A. T. M. Nicolai, Bart M. Rudell, David R. TI Gene expression and metabolism preceding soft scald, a chilling injury of 'Honeycrisp' apple fruit SO BMC GENOMICS LA English DT Article DE Malus x domestica Borkh; Apples; Transcriptomics; Metabolomics; Chilling injury; Soft scald ID ALPHA-FARNESENE SYNTHASE; X DOMESTICA BORKH.; SUPERFICIAL SCALD; ISOPRENOID BIOSYNTHESIS; ANTIOXIDANT TREATMENT; CONTROLLED-ATMOSPHERE; STORAGE-TEMPERATURE; FUNCTIONAL-DISORDER; PLASTIDIAL PATHWAYS; OXIDATION-PRODUCTS AB Background: 'Honeycrisp' is an apple cultivar that is susceptible to soft scald, a chilling injury expressed as necrotic patches on the peel. Improved understanding of metabolism associated with the disorder would improve our understanding of soft scald and contribute to developing more effective management strategies for apple storage. It was expected that specific gene expression and specific metabolite levels in the peel would be linked with soft scald risk at harvest and/or specific time points during cold storage. Results: Fruit from nine 'Honeycrisp' apple orchards that would eventually develop different incidences of soft scald between 4 and 8 weeks of cold air storage were used to contrast and determine differential transcriptomic and metabolomic changes during storage. Untargeted metabolic profiling revealed changes in a number of distinct pathways preceding and concurrent with soft scald symptom development, including elevated gamma-aminobutryic acid (GABA), 1-hexanol, acylated steryl glycosides, and free rho-coumaryl acyl esters. At harvest, levels of sesquiterpenoid and triterpenoid acyl esters were relatively higher in peel of fruit that did not later develop the disorder. RNA-seq driven gene expression profiling highlighted possible involvement of genes and associated metabolic processes with soft scald development. These included elevated expression of genes involved in lipid peroxidation and phenolic metabolism in fruit with soft scald, and isoprenoid/brassinosteroid metabolism in fruit that did not develop soft scald. Expression of other stress-related genes in fruit that developed soft scald included chlorophyll catabolism, cell wall loosening, and lipid transport while superoxide dismutases were up-regulated in fruit that did not develop the disorder. Conclusions: This study delineates the sequential transcriptomic and metabolomic changes preceding soft scald symptom development. Changes were differential depending on susceptibility of fruit to the disorder and could be attributed to key stress related and mediating pathways. C1 [Leisso, Rachel S.; Mattheis, James P.; Sullivan, Nathanael L.; Rudell, David R.] ARS, USDA, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. [Gapper, Nigel E.; Watkins, Christopher B.] Cornell Univ, Hort Sect, Sch Plant Sci, Ithaca, NY 14853 USA. [Gapper, Nigel E.] AgroFresh Solut Inc, 130 Technol Ctr Way Wenatchee, Wenatchee, WA 98801 USA. [Giovannoni, James J.] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Giovannoni, James J.] ARS, USDA, Plant Soil & Nutr Lab, Ithaca, NY 14853 USA. [Schaffer, Robert J.] Plant & Food Res Ltd, New Zealand Inst, Auckland, New Zealand. [Johnston, Jason W.] Plant & Food Res Ltd, New Zealand Inst, Havelock North, New Zealand. [Hanrahan, Ines] Washington Tree Fruit Res Commiss, Wenatchee, WA USA. [Hertog, Maarten L. A. T. M.; Nicolai, Bart M.] Katholieke Univ Leuven, BIOSYST MeBioS, Heverlee, Belgium. RP Rudell, DR (reprint author), ARS, USDA, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. EM David.Rudell@ars.usda.gov FU USDA Specialty Crops Research Initiative [2010-51181-21446]; Washington Tree Fruit Research Commission; AgroFresh, Inc. FX This research was supported by the USDA Specialty Crops Research Initiative (Project number 2010-51181-21446), the Washington Tree Fruit Research Commission, and AgroFresh, Inc. NR 87 TC 0 Z9 0 U1 21 U2 21 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD OCT 12 PY 2016 VL 17 AR 798 DI 10.1186/s12864-016-3019-1 PG 23 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DZ0EN UT WOS:000385510400006 PM 27733113 ER PT J AU Lin, M Zhang, DD Liu, SB Zhang, GR Yu, JM Fritz, AK Bai, GH AF Lin, Meng Zhang, Dadong Liu, Shubing Zhang, Guorong Yu, Jianming Fritz, Allan K. Bai, Guihua TI Genome-wide association analysis on pre-harvest sprouting resistance and grain color in US winter wheat SO BMC GENOMICS LA English DT Article DE Triticum aestivum; pre-harvest sprouting resistance; grain color; genome- wide association studies ID QUANTITATIVE TRAIT LOCI; TRITICUM-AESTIVUM L.; SEED COAT COLOR; LINKAGE DISEQUILIBRIUM; POPULATION-STRUCTURE; GENETIC COMPONENTS; HEXAPLOID WHEAT; DORMANCY; DIVERSITY; QTL AB Background: Pre-harvest sprouting (PHS) in wheat can cause substantial reduction in grain yield and end-use quality. Grain color (GC) together with other components affect PHS resistance. Several quantitative trait loci (QTL) have been reported for PHS resistance, and two of them on chromosome 3AS (TaPHS1) and 4A have been cloned. Methods: To determine genetic architecture of PHS and GC and genetic relationships of the two traits, a genome-wide association study (GWAS) was conducted by evaluating a panel of 185 U.S. elite breeding lines and cultivars for sprouting rates of wheat spikes and GC in both greenhouse and field experiments. The panel was genotyped using the wheat 9K and 90K single nucleotide polymorphism (SNP) arrays. Results: Four QTL for GC on four chromosomes and 12 QTL for PHS resistance on 10 chromosomes were identified in at least two experiments. QTL for PHS resistance showed varied effects under different environments, and those on chromosomes 3AS, 3AL, 3B, 4AL and 7A were the more frequently identified QTL. The common QTL for GC and PHS resistance were identified on the long arms of the chromosome 3A and 3D. Conclusions: Wheat grain color is regulated by the three known genes on group 3 chromosomes and additional genes from other chromosomes. These grain color genes showed significant effects on PHS resistance in some environments. However, several other QTL that did not affect grain color also played a significant role on PHS resistance. Therefore, it is possible to breed PHS-resistant white wheat by pyramiding these non-color related QTL. C1 [Lin, Meng; Zhang, Dadong; Liu, Shubing; Zhang, Guorong; Fritz, Allan K.; Bai, Guihua] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Yu, Jianming] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Bai, Guihua] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. RP Bai, GH (reprint author), Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA.; Bai, GH (reprint author), USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. EM gbai@ksu.edu FU National Research Initiative Competitive Grants CAP Project from the USDA National Institute of Food and Agriculture [2011-68002-30029] FX This study was partially funded by the National Research Initiative Competitive Grants CAP Project (2011-68002-30029) from the USDA National Institute of Food and Agriculture. NR 62 TC 1 Z9 1 U1 19 U2 19 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD OCT 12 PY 2016 VL 17 AR 794 DI 10.1186/s12864-016-3148-6 PG 16 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DZ0EN UT WOS:000385510400002 PM 27729004 ER PT J AU Cheng, TYD Shelver, WL Hong, CC McCann, SE Davis, W Zhang, YL Ambrosone, CB Smith, DJ AF Cheng, Ting-Yuan David Shelver, Weilin L. Hong, Chi-Chen McCann, Susan E. Davis, Warren Zhang, Yali Ambrosone, Christine B. Smith, David J. TI Urinary Excretion of the beta-Adrenergic Feed Additives Ractopamine and Zilpaterol in Breast and Lung Cancer Patients SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE beta(2)-adrenergic agonists; ractopamine; zilpaterol; urine; cancer; meat consumption ID TISSUE RESIDUES; HEALTH CONCERNS; AGONISTS; CLENBUTEROL; LIVESTOCK; PERFORMANCE; METABOLISM; ANIMALS; AGENT; WATER AB beta(2)-Adrenergic agonists (beta-agonists) have been legally used in the U.S. for almost two decades to increase lean muscle mass in meat animals. Despite a cardiotoxic effect after high-dose exposure, there has been limited research on human beta-agonist exposures related to meat consumption. We quantified urinary concentrations of ractopamine and zilpaterol, two FDA approved beta-agonist feed additives, and examined the extent to which the concentrations were associated with estimated usual meat intake levels. Overnight urine samples from 324 newly diagnosed breast cancer patients and spot urine samples from 46 lung cancer patients at the time of diagnosis, prior to treatment, were collected during 2006-2010 and 2014-2015, respectively. Urinary ractopamine and zilpaterol concentrations were measured by LC-MS/MS. Ractopamine and zilpaterol, respectively, were detected in 8.1% and 3.0% of the urine samples collected (n = 370). Only 1.1% (n = 4) of the urine samples had zilpaterol concentrations above the limit of quantification, with the mean value of 0.07 ng/mL in urine. The presence of detectable ractopamine and zilpaterol levels were not associated with meat consumption estimated from a food frequency questionnaire, including total meat (P = 0.13 and 0.74, respectively), total red meat (P = 0.72 and 0.74), unprocessed red meat (P = 0.74 and 0.73), processed red meat (P = 0.72 and 0.15), and poultry intake (P = 0.67 for ractopamine). Our data suggest that the amount of meat-related exposure of beta-agonists was low. C1 [Cheng, Ting-Yuan David; Hong, Chi-Chen; McCann, Susan E.; Davis, Warren; Zhang, Yali; Ambrosone, Christine B.] Roswell Pk Canc Inst, Dept Canc Prevent & Control, Elm & Carlton St, Buffalo, NY 14263 USA. [Shelver, Weilin L.; Smith, David J.] USDA ARS, Biosci Res Lab, Fargo, ND 58103 USA. RP Cheng, TYD (reprint author), Roswell Pk Canc Inst, Dept Canc Prevent & Control, Elm & Carlton St, Buffalo, NY 14263 USA. EM david.cheng@roswellpark.org FU Susan G. Komen Breast Cancer Foundation; Breast Cancer Research Foundation [BCTR104906]; U.S. Army Medical Research and Materiel Command [DoD W81XWH0610401]; RPCI Alliance Foundation; RPCI's Cancer Center Support Grant from the National Cancer Institute [P30CA016056] FX The Women's Health after Breast Cancer (ABC) Study was supported by Susan G. Komen Breast Cancer Foundation. (BCTR104906), Breast Cancer Research Foundation, the U.S. Army Medical Research and Materiel Command (DoD W81XWH0610401), and RPCI Alliance Foundation. DBBR is supported by RPCI's Cancer Center Support Grant from the National Cancer Institute (P30CA016056). NR 45 TC 0 Z9 0 U1 4 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD OCT 12 PY 2016 VL 64 IS 40 BP 7632 EP 7639 DI 10.1021/acs.jafc.6b02723 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DY9QJ UT WOS:000385469900026 PM 27641640 ER PT J AU Reyes-Fox, M Steltzer, H LeCain, DR McMaster, GS AF Reyes-Fox, Melissa Steltzer, Heidi LeCain, Daniel R. McMaster, Gregory S. TI Five years of phenology observations from a mixed-grass prairie exposed to warming and elevated CO2 SO SCIENTIFIC DATA LA English DT Article; Data Paper ID CARBON-DIOXIDE; ATMOSPHERIC CO2; GLOBAL CHANGE; ENRICHMENT; TEMPERATURE; RESPONSES; ECOSYSTEM; GROWTH AB Atmospheric CO2 concentrations have been steadily increasing since the Industrial Era and contribute to concurrent increases in global temperatures. Many observational studies suggest climate warming alone contributes to a longer growing season. To determine the relative effect of warming on plant phenology, we investigated the individual and joint effects of warming and CO2 enrichment on a mixed-grass prairie plant community by following the development of six common grassland species and recording four major life history events. Our data support that, in a semi-arid system, while warming advances leaf emergence and flower production, it also expedites seed maturation and senescence at the species level. However, the additive effect can be an overall lengthening of the growing and reproductive seasons since CO2 enrichment, particularly when combined with warming, contributed to a longer growing season by delaying plant maturation and senescence. Fostering synthesis across multiple phenology datasets and identifying key factors affecting plant phenology will be vital for understanding regional plant community responses to climate change. C1 [Reyes-Fox, Melissa] USDA ARS, Soil Plant Nutrient Res Unit, Ft Collins, CO 80526 USA. [Reyes-Fox, Melissa; McMaster, Gregory S.] Northern Plains Area, Ft Collins, CO 80526 USA. [Steltzer, Heidi] Ft Lewis Coll, Dept Biol, Durango, CO 81301 USA. [LeCain, Daniel R.] USDA ARS, Rangeland Resources Res Unit, Ft Collins, CO 80526 USA. [McMaster, Gregory S.] USDA ARS, Agr Syst Res Unit, Ft Collins, CO 80526 USA. RP Reyes-Fox, M (reprint author), USDA ARS, Soil Plant Nutrient Res Unit, Ft Collins, CO 80526 USA.; Reyes-Fox, M (reprint author), Northern Plains Area, Ft Collins, CO 80526 USA.; Steltzer, H (reprint author), Ft Lewis Coll, Dept Biol, Durango, CO 81301 USA. EM melissa.reyes@ars.usda.gov; steltzer_h@fortlewis.edu NR 26 TC 0 Z9 0 U1 6 U2 6 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2052-4463 J9 SCI DATA JI Sci. Data PD OCT 11 PY 2016 VL 3 AR UNSP 160088 DI 10.1038/sdata.2016.88 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EF3ON UT WOS:000390234100002 PM 27727235 ER PT J AU Belamkar, V Farmer, AD Weeks, NT Kalberer, SR Blackmon, WJ Cannon, SB AF Belamkar, Vikas Farmer, Andrew D. Weeks, Nathan T. Kalberer, Scott R. Blackmon, William J. Cannon, Steven B. TI Genomics-assisted characterization of a breeding collection of Apios americana, an edible tuberous legume SO SCIENTIFIC REPORTS LA English DT Article ID REDUCED REPRESENTATION LIBRARIES; SINGLE NUCLEOTIDE POLYMORPHISM; THROUGHPUT SNP DISCOVERY; ASSOCIATION; GENERATION; SEQUENCE; QUINOA; TUBERIZATION; LEGUMINOSAE; EXPRESSION AB For species with potential as new crops, rapid improvement may be facilitated by new genomic methods. Apios (Apios americana Medik.), once a staple food source of Native American Indians, produces protein-rich tubers, tolerates a wide range of soils, and symbiotically fixes nitrogen. We report the first high-quality de novo transcriptome assembly, an expression atlas, and a set of 58,154 SNP and 39,609 gene expression markers (GEMs) for characterization of a breeding collection. Both SNPs and GEMs identify six genotypic clusters in the collection. Transcripts mapped to the Phaseolus vulgaris genome-another phaseoloid legume with the same chromosome number-provide provisional genetic locations for 46,852 SNPs. Linkage disequilibrium decays within 10 kb (based on the provisional genetic locations), consistent with outcrossing reproduction. SNPs and GEMs identify more than 21 marker-trait associations for at least 11 traits. This study demonstrates a holistic approach for mining plant collections to accelerate crop improvement. C1 [Belamkar, Vikas] Iowa State Univ, Interdept Genet, Ames, IA 50011 USA. [Belamkar, Vikas; Cannon, Steven B.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Farmer, Andrew D.] Natl Ctr Genome Resources, Santa Fe, NM 87505 USA. [Weeks, Nathan T.; Kalberer, Scott R.; Cannon, Steven B.] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Blackmon, William J.] Louisiana State Univ, Ctr Agr, Louisiana Agr Expt Stn, Dept Hort, Baton Rouge, LA 70803 USA. [Belamkar, Vikas] Univ Nebraska Lincoln, Dept Agron & Hort, Lincoln, NE 68583 USA. [Blackmon, William J.] 5097 Studley Rd, Mechanicsville, VA 23116 USA. RP Cannon, SB (reprint author), Iowa State Univ, Dept Agron, Ames, IA 50011 USA.; Cannon, SB (reprint author), USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. EM steven.cannon@ars.usda.gov NR 61 TC 0 Z9 0 U1 6 U2 6 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD OCT 10 PY 2016 VL 6 AR 34908 DI 10.1038/srep34908 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DY5NT UT WOS:000385147200001 PM 27721469 ER PT J AU Glenn, DM AF Glenn, D. M. TI Dry matter partitioning and photosynthetic response to biennial bearing and freeze damage in 'Empire' apple SO SCIENTIA HORTICULTURAE LA English DT Article DE Malus domestica; Radiation use efficiency; Gas exchange; Fruit size; Leaf:fruit ratio ID CROP LOAD; GAS-EXCHANGE; FRUIT SIZE; LEAF PHOTOSYNTHESIS; VEGETATIVE GROWTH; DARK RESPIRATION; DELICIOUS APPLE; RETURN BLOOM; TREES; CANOPY AB Biennial cropping is typically characterized as having 'ON' and 'OFF' years in which the 'ON' year produces an excessive crop load and the 'OFF' year has a very small crop load. Frost damage during the bloom period or winter freeze damage is another means of reducing the crop load that can initiate biennial bearing in an otherwise consistent cropping system. The objectives were to determine what effect single and multiple year crop loss had on the current and subsequent year's dry matter partitioning and photosynthesis. Dry matter partitioning and whole tree photosynthesis (A) were being measured in biennial bearing 'Empire' apple trees when 2 consecutive frosts occurred following an 'OFF' year. This provided an opportunity to evaluate two cycles of conventional biennial bearing and the effect of 3 years of suppressed crop load on dry matter partitioning and whole tree photosynthesis. Frost events reduced fruit dry weight to levels equivalent to the 'OFF' years but the 'SUPPRESSED' years (1 'OFF' and 1 or 2 frost years) reduced partitioning to wood dry matter and leaf + fruit more than the 'OFF' years while increasing the fruit dry matter partitioning compared to the 'OFF' years despite similar fruit yield and crop load. The 'ON' years had the highest fruit dry weight and% dry weight partitioned to fruit and leaf + fruit. Conversely, the 'ON' year had the least leaf area index (LAI) and% dry weight partitioned to wood and leaf tissue. There were no differences in canopy area, however, the 'ON' years had a significantly reduced LAI. Radiation use efficiency RUE; Annual biomass (kg)/PAR [(MegaMoles/growing season) x canopy area (m(2))] was linearly correlated with the% dry mass partitioned to fruit and negatively correlated with LAI. For a specified LAI, the 'ON' year had a greater RUE than the 'OFF' or 'SUPPRESSED' years and there were no differences between 'OFF' and 'SUPPRESSED' years. Analysis of daily photosynthesis (A) indicated that all three treatments were significantly different. The 'ON' years had the highest mean daily A (3.8 mu mol/m(2)/s), 'SUPPRESSED' years were intermediate (3.0 mu mol/m(2)/s) and the 'OFF' years had the lowest A (2.5 mu mol/m(2)/s). It appears that in years when fruiting is suppressed more than a single year, dry matter partitioning to wood is also suppressed and a great partitioning occurs to the remaining fruit and leaf tissue. This may be a response to a continued deficit of the hormonal complex supplied by fruit as they develop. Published by Elsevier B.V. C1 [Glenn, D. M.] USDA, ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. RP Glenn, DM (reprint author), USDA, ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM michael.glenn@ars.usda.gov NR 37 TC 0 Z9 0 U1 10 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD OCT 10 PY 2016 VL 210 BP 1 EP 5 DI 10.1016/j.scienta.2016.06.042 PG 5 WC Horticulture SC Agriculture GA DX4YW UT WOS:000384388000001 ER PT J AU Cruz, JL Alves, AAC LeCain, DR Ellis, DD Morgan, JA AF Cruz, Jailson L. Alves, Alfredo A. C. LeCain, Daniel R. Ellis, David D. Morgan, Jack A. TI Elevated CO2 concentrations alleviate the inhibitory effect of drought on physiology and growth of cassava plants SO SCIENTIA HORTICULTURAE LA English DT Article DE Water deficit; Gas exchange; Water-use efficiency; Transpiration; Biomass ID ATMOSPHERIC CARBON-DIOXIDE; WATER-USE EFFICIENCY; FOREST FACE SITES; CLIMATE-CHANGE; GAS-EXCHANGE; PHOTOSYNTHETIC LIMITATIONS; MEDITERRANEAN PLANTS; MANIHOT-ESCULENTA; TEMPERATE FOREST; FOOD SECURITY AB Due to the rise in anthropogenic greenhouse gas concentrations, the earth's climate is expected to change, with precipitation being reduced in some areas resulting in growth-limiting drought and, as a consequence, reduced plant productivity. We investigated the physiological and growth responses of cassava (Manihot esculenta Crantz) to approximate present-day ambient (390 mu L L-1) and elevated (750 mu L L-1) atmospheric CO2 concentrations under well-watered and water deficit conditions, aiming at understanding how cassava would face those problems. Water deficits led to reductions in the Leaf Elongation Rate of plants grown at ambient as well as CO2-enriched concentrations. However, plants grown at 750 mu L L-1 of CO2 maintained leaf growth two days longer than plants grown at 390 mu L L-1. Three Days After Withholding Water (DAWW), photosynthesis and stomatal conductance were-reduced in plants grown under ambient CO2, while in plants under an elevated CO2 concentration, these physiological functions remained similar to that of control plants grown under good water availability. Five DAWW plants grown with 750 mu L L-1 continued to have enhanced gas exchange compared with plants grown under 390 mu L L-1. Under drought stress, the instantaneous transpiration efficiency was always greatest for plants grown under elevated CO2, The positive response of elevated CO2 levels on total dry mass was 61% in the water-stressed plants and only 20% for the plants grown under good water availability. Stomatal limitation was an important factor reducing CO2 assimilation in cassava growing under drought conditions. 2016 Elsevier B.V. All rights reserved. C1 [Cruz, Jailson L.; Alves, Alfredo A. C.] Embrapa Mandioca & Fruticultura, Cruz Das Almas, BA, Brazil. [LeCain, Daniel R.; Morgan, Jack A.] USDA ARS, Crops Res Lab, 1701 Ctr Ave, Ft Collins, CO 80526 USA. [Ellis, David D.] Int Potato Ctr, Ave La Molina 1895, Lima, Peru. RP Cruz, JL (reprint author), Embrapa Mandioca & Fruticultura, Cruz Das Almas, BA, Brazil. EM jailson.cruz@embrapa.br FU United States Department of Agriculture/USDA-ARS; Brazilian Agricultural Research Corporation (Embrapa) FX This research was partially supported by United States Department of Agriculture/USDA-ARS and Brazilian Agricultural Research Corporation (Embrapa). The authors gratefully acknowledge Deborah P. Cruz and Maria Claudia Pelacani for kind help in growing plants. Also, thanks to Leonardo Zebral Rodrigues by providing in vitro plantlets. NR 62 TC 0 Z9 0 U1 64 U2 64 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD OCT 10 PY 2016 VL 210 BP 122 EP 129 DI 10.1016/j.scienta.2016.07.012 PG 8 WC Horticulture SC Agriculture GA DX4YW UT WOS:000384388000015 ER PT J AU Zhou, Y Xu, LY Bickhart, DM Hay, EA Schroeder, SG Connor, EE Alexander, LJ Sonstegard, TS Van Tassell, CP Chen, H Liu, GE AF Zhou, Yang Xu, Lingyang Bickhart, Derek M. Hay, El Hamidi abdel Schroeder, Steven G. Connor, Erin E. Alexander, Leeson J. Sonstegard, Tad S. Van Tassell, Curtis P. Chen, Hong Liu, George E. TI Reduced representation bisulphite sequencing of ten bovine somatic tissues reveals DNA methylation patterns and their impacts on gene expression SO BMC GENOMICS LA English DT Article DE Cattle genome; Somatic tissue; DNA methylation; RRBS (reduced representation bisulphite sequencing) ID GENOME-WIDE ANALYSIS; GERM-CELL DEVELOPMENT; EMBRYONIC STEM-CELLS; NON-CPG METHYLATION; ARABIDOPSIS; SEQ; TRANSCRIPTION; ANNOTATION; EVOLUTION; PROFILES AB Background: As a major epigenetic component, DNA methylation plays important functions in individual development and various diseases. DNA methylation has been well studied in human and model organisms, but only limited data exist in economically important animals like cattle. Results: Using reduced representation bisulphite sequencing (RRBS), we obtained single-base-resolution maps of bovine DNA methylation from ten somatic tissues. In total, we evaluated 1,868,049 cytosines in CG-enriched regions. While we found slightly low methylation levels (29.87 to 38.06 %) in cattle, the methylation contexts (CGs and non-CGs) of cattle showed similar methylation patterns to other species. Non-CG methylation was detected but methylation levels in somatic tissues were significantly lower than in pluripotent cells. To study the potential function of the methylation, we detected 10,794 differentially methylated cytosines (DMCs) and 836 differentially methylated CG islands (DMIs). Further analyses in the same tissues revealed many DMCs (including non-CGs) and DMIs, which were highly correlated with the expression of genes involved in tissue development. Conclusions: In summary, our study provides a baseline dataset and essential information for DNA methylation profiles of cattle. C1 [Zhou, Yang; Chen, Hong] Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Agr Mol Biol, Yangling 712100, Shaanxi, Peoples R China. [Zhou, Yang; Bickhart, Derek M.; Schroeder, Steven G.; Connor, Erin E.; Van Tassell, Curtis P.; Liu, George E.] USDA ARS, Anim Genom & Improvement Lab, BARC, Bldg 306,Room 111,BARC East, Beltsville, MD 20705 USA. [Xu, Lingyang] Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China. [Hay, El Hamidi abdel; Alexander, Leeson J.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. [Sonstegard, Tad S.] Recombinetics Inc, St Paul, MN 55108 USA. RP Chen, H (reprint author), Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Agr Mol Biol, Yangling 712100, Shaanxi, Peoples R China.; Liu, GE (reprint author), USDA ARS, Anim Genom & Improvement Lab, BARC, Bldg 306,Room 111,BARC East, Beltsville, MD 20705 USA. EM chenhong1212@263.net; George.Liu@ars.usda.gov FU AFRI grant from the USDA National Institute of Food and Agriculture (NIFA) [2011-67015-30183, 2013-67015-20951] FX This work was supported in part by AFRI grant numbers 2011-67015-30183 and 2013-67015-20951 from the USDA National Institute of Food and Agriculture (NIFA) Animal Genome and Reproduction Programs. NR 59 TC 0 Z9 0 U1 2 U2 2 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD OCT 6 PY 2016 VL 17 AR 779 DI 10.1186/s12864-016-3116-1 PG 11 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DY3HW UT WOS:000384982700001 PM 27716143 ER PT J AU Abdelfattah, A Wisniewski, M Droby, S Schena, L AF Abdelfattah, Ahmed Wisniewski, Michael Droby, Samir Schena, Leonardo TI Spatial and compositional variation in the fungal communities of organic and conventionally grown apple fruit at the consumer point-of-purchase SO HORTICULTURE RESEARCH LA English DT Article ID SCANNING-ELECTRON-MICROSCOPY; CORE ROT; METSCHNIKOWIA-PULCHERRIMA; ACREMONIUM-ALTERNATUM; POPULATION-DYNAMICS; ENDOPHYTIC FUNGUS; PENICILLIUM SPP.; TABLE GRAPES; BROWN SPOT; MOLDY-CORE AB The fungal diversity in harvested apples from organic or conventional management practices was analyzed in different fruit locations (stem end, calyx end, peel, and wounded flesh) shortly after fruit purchase (T1) and after 2 weeks of storage (T5). A total of 5,760,162 high-quality fungal sequences were recovered and assigned to 8,504 Operational Taxonomic Units. Members of the phylum Ascomycota were dominant in all samples and accounted for 91.6% of the total number of detected sequences. This was followed by Basidiomycota (8%), Chytridiomycota (0.1%), and unidentified fungi (0.3%). Alpha and beta diversity analyses revealed the presence of significantly different fungal populations in the investigated fruit parts. Among detected fungi, the genus Penicillium prevailed in the peel and in the wounded flesh while Alternaria spp. prevailed in the calyx and stem end samples that included apple core tissues. Several taxonomic units that appear to be closely related to pathogenic fungi associated with secondary human infections were present in peel and wounds. Moreover, significantly different populations were revealed in organic and conventional apples and this result was consistent in all investigated fruit parts (calyx end, peel, stem end, and wounded flesh). Several unique taxa were exclusively detected in organic apples suggesting that management practices may have been a contributing factor in determining the taxa present. In contrast, little differences were revealed in the two assessment times (T1 and T5). Results of the present study represent an advancement of the current knowledge on the fungal microbiota in collected fruit tissues of apple. C1 [Abdelfattah, Ahmed; Schena, Leonardo] Univ Mediterranea Reggio Calabria, Dipartimento Agr, I-89124 Calabria, Italy. [Wisniewski, Michael] ARS, USDA, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. [Droby, Samir] ARO, Volcani Ctr, Dept Postharvest & Food Sci, 68 HaMccabim Rd, IL-7505101 Rishon Leziyyon, Israel. RP Schena, L (reprint author), Univ Mediterranea Reggio Calabria, Dipartimento Agr, I-89124 Calabria, Italy.; Wisniewski, M (reprint author), ARS, USDA, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM Michael.Wisniewski@ars.usda.gov; lschena@unirc.it OI Abdelfattah, Ahmed/0000-0001-6090-7200 NR 91 TC 0 Z9 0 U1 11 U2 11 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2052-7276 J9 HORTIC RES-ENGLAND JI Hortic. Res.-England PD OCT 5 PY 2016 VL 3 AR 16047 DI 10.1038/hortres.2016.47 PG 12 WC Plant Sciences; Horticulture SC Plant Sciences; Agriculture GA DY9FJ UT WOS:000385438800001 PM 27766161 ER PT J AU Girard, AL Castell-Perez, ME Bean, SR Adrianos, SL Awika, JM AF Girard, Audrey L. Castell-Perez, M. Elena Bean, Scott R. Adrianos, Sherry L. Awika, Joseph M. TI Effect of Condensed Tannin Profile on Wheat Flour Dough Rheology SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE tannin; proanthocyanidin; wheat gluten; dough rheology ID IN-VITRO STARCH; GLUTEN PROTEINS; PHENOLIC-COMPOUNDS; MIXING PROPERTIES; TORTILLA QUALITY; BREAD QUALITY; DIGESTIBILITY; PROCYANIDIN; SPECIFICITY; REDUCTION AB Proanthocyanidins (PA) cross-link proteins and could expand wheat gluten functionality; however, how the PA MW or gluten profile affect these interactions is unknown. Effect of PA MW profile (sorghum versus grape seed PA) on dough rheology of high versus low insoluble polymeric protein (IPP) wheat flour was evaluated using mixograph, large (TA.XT2i) and small (HAAKE Rheostress 6000) deformation rheometry. Sorghum PA (93% polymeric) more effectively (p < 0.05) strengthened both glutens than grape seed PA (45% polymeric), without reducing gluten extensibility. These effects were higher in low IPP (weak gluten) flour, e.g., sorghum PA doubled IPP, increased mix time by 75%, dough elasticity by 82%, and peak angle by 17 degrees versus control. Grape seed PA increased IPP by 75% and elasticity by 36%, but reduced peak angle by 15 degrees, indicating reduced mixing tolerance. Sorghum PA, but not grape seed PA, increased (p < 0.05) all above parameters in high IPP dough. Polymeric PA more effectively strengthened gluten than oligomeric PA, likely via more efficient protein cross-linking to overcome strong antioxidant effect of PA. High MW PA may be useful natural gluten strengtheners for diverse applications. C1 [Girard, Audrey L.; Awika, Joseph M.] Texas A&M Univ, Dept Soil & Crop Sci, 2474 TAMU, College Stn, TX 77843 USA. [Girard, Audrey L.; Awika, Joseph M.] Texas A&M Univ, Dept Food Sci & Nutr, College Stn, TX 77843 USA. [Castell-Perez, M. Elena] Texas A&M Univ, Dept Biol & Agr Engn, College Stn, TX 77843 USA. [Bean, Scott R.; Adrianos, Sherry L.] USDA ARS, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. RP Awika, JM (reprint author), Texas A&M Univ, Dept Soil & Crop Sci, 2474 TAMU, College Stn, TX 77843 USA.; Awika, JM (reprint author), Texas A&M Univ, Dept Food Sci & Nutr, College Stn, TX 77843 USA. EM awika@tamu.edu NR 37 TC 0 Z9 0 U1 17 U2 17 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD OCT 5 PY 2016 VL 64 IS 39 BP 7348 EP 7356 DI 10.1021/acs.jafc.6b02601 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DY2VZ UT WOS:000384951500006 PM 27616442 ER PT J AU Beaudart, C McCloskey, E Bruyere, O Cesari, M Rolland, Y Rizzoli, R de Carvalho, IA Thiyagarajan, JA Bautmans, I Bertiere, MC Brandi, ML Al-Daghri, NM Burlet, N Cavalier, E Cerreta, F Cherubini, A Fielding, R Gielen, E Landi, F Petermans, J Reginster, JY Visser, M Kanis, J Cooper, C AF Beaudart, Charlotte McCloskey, Eugene Bruyere, Olivier Cesari, Matteo Rolland, Yves Rizzoli, Rene de Carvalho, Islene Araujo Thiyagarajan, Jotheeswaran Amuthavalli Bautmans, Ivan Bertiere, Marie-Claude Brandi, Maria Luisa Al-Daghri, Nasser M. Burlet, Nansa Cavalier, Etienne Cerreta, Francesca Cherubini, Antonio Fielding, Roger Gielen, Evelien Landi, Francesco Petermans, Jean Reginster, Jean-Yves Visser, Marjolein Kanis, John Cooper, Cyrus TI Sarcopenia in daily practice: assessment and management SO BMC GERIATRICS LA English DT Article DE Sarcopenia; Daily practice; Assessment; Management; Tools ID X-RAY ABSORPTIOMETRY; ANTHROPOMETRIC PREDICTION EQUATIONS; BIOELECTRICAL-IMPEDANCE ANALYSIS; APPENDICULAR SKELETAL-MUSCLE; DWELLING OLDER-PEOPLE; LEAN SOFT-TISSUE; OSTEOARTHRITIS ESCEO; PHYSICAL PERFORMANCE; EUROPEAN-SOCIETY; ECONOMIC-ASPECTS AB Background: Sarcopenia is increasingly recognized as a correlate of ageing and is associated with increased likelihood of adverse outcomes including falls, fractures, frailty and mortality. Several tools have been recommended to assess muscle mass, muscle strength and physical performance in clinical trials. Whilst these tools have proven to be accurate and reliable in investigational settings, many are not easily applied to daily practice. Methods: This paper is based on literature reviews performed by members of the European Society for Clinical and Economic Aspects of Osteoporosis and Osteoarthritis (ESCEO) working group on frailty and sarcopenia. Face-to-face meetings were afterwards organized for the whole group to make amendments and discuss further recommendations. Results: This paper proposes some user-friendly and inexpensive methods that can be used to assess sarcopenia in real-life settings. Healthcare providers, particularly in primary care, should consider an assessment of sarcopenia in individuals at increased risk; suggested tools for assessing risk include the Red Flag Method, the SARC-F questionnaire, the SMI method or different prediction equations. Management of sarcopenia should primarily be patient centered and involve the combination of both resistance and endurance based activity programmes with or without dietary interventions. Development of a number of pharmacological interventions is also in progress. Conclusions: Assessment of sarcopenia in individuals with risk factors, symptoms and/or conditions exposing them to the risk of disability will become particularly important in the near future. C1 [Beaudart, Charlotte; Bruyere, Olivier; Burlet, Nansa; Reginster, Jean-Yves] Univ Liege, Dept Publ Hlth Epidemiol & Hlth Econ, Ave Hippocrate 13,CHU B23, B-4000 Liege, Belgium. [McCloskey, Eugene; Kanis, John] Univ Sheffield, Ctr Metab Bone Dis, Sheffield, S Yorkshire, England. [McCloskey, Eugene] MRC & Arthrit Res UK Ctr Integrated Res Musculosk, London, England. [Cesari, Matteo] Univ Hosp Toulouse, Gerontopole, Toulouse, France. [Cesari, Matteo] Univ Toulouse 3, INSERM, UMR1027, Toulouse, France. [Rolland, Yves] Univ Toulouse 3, CHU Purpan, Gerontopole Toulouse, Toulouse, France. [Rizzoli, Rene] Univ Hosp Geneva, Fac Med, Serv Bone Dis, Geneva, Switzerland. [de Carvalho, Islene Araujo; Thiyagarajan, Jotheeswaran Amuthavalli] WHO, Geneva, Switzerland. [Bautmans, Ivan] Vrije Univ Brussel, Gerontol & Frailty Ageing Res Dept, Brussels, Belgium. [Bertiere, Marie-Claude] Ctr Rech & Informat Nutritionnelles, Paris, France. [Brandi, Maria Luisa] Univ Florence, Dept Surg & Translat Med, Viale Pieraccini 6, I-59139 Florence, Italy. [Al-Daghri, Nasser M.] King Saud Univ, Coll Sci, Dept Biochem, Prince Mutaib Chair Biomarkers Osteoporosis, Riyadh 11451, Saudi Arabia. [Cavalier, Etienne] Univ Liege, CHU Sart Tilman, Dept Clin Chem, Bat B35, B-4000 Liege, Belgium. [Cerreta, Francesca] Human Med Res & Dev Support Div, Sci Advice, London, England. [Cherubini, Antonio] IRCCS INRCA, Geriatr & Geriatr Emergency Care, Ancona, Italy. [Fielding, Roger] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. [Gielen, Evelien] Katholieke Univ Leuven, Dept Geriatr Med, Leuven, Belgium. [Landi, Francesco] Catholic Univ Sacred Heart Rome, Dept Geriatr Neurosci & Orthoped, Milan, Italy. [Petermans, Jean] CHU Sart Tilman, Dept Geriatr, Bat B35, B-4000 Liege, Belgium. [Visser, Marjolein] Vrije Univ Amsterdam, Dept Hlth Sci, Amsterdam, Netherlands. [Visser, Marjolein] Vrije Univ Amsterdam, Med Ctr, Dept Nutr & Dietet, Internal Med, Amsterdam, Netherlands. [Kanis, John] Catholic Univ Australia, Inst Hlth & Aging, Melbourne, Vic, Australia. [Cooper, Cyrus] Univ Southampton, MRC Lifecourse Epidemiol Unit, Southampton, Hants, England. [Cooper, Cyrus] Univ Oxford, NIHR Musculoskeletal Biomed Res Unit, Oxford, England. RP Beaudart, C (reprint author), Univ Liege, Dept Publ Hlth Epidemiol & Hlth Econ, Ave Hippocrate 13,CHU B23, B-4000 Liege, Belgium. EM c.beaudart@ulg.ac.be RI Cesari, Matteo/A-4649-2008 OI Cesari, Matteo/0000-0002-0348-3664 FU Medical Research Council [MC_UP_A620_1014, MC_U147585819, MC_UU_12011/2, MC_UP_A620_1015, MC_UU_12011/1] NR 83 TC 1 Z9 1 U1 20 U2 20 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2318 J9 BMC GERIATR JI BMC Geriatr. PD OCT 5 PY 2016 VL 16 AR 170 DI 10.1186/s12877-016-0349-4 PG 10 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA DX9FS UT WOS:000384699600001 PM 27716195 ER PT J AU Margolis, LM Rivas, DA Berrone, M Ezzyat, Y Young, AJ McClung, JP Fielding, RA Pasiakos, SM AF Margolis, Lee M. Rivas, Donato A. Berrone, Maria Ezzyat, Yassine Young, Andrew J. McClung, James P. Fielding, Roger A. Pasiakos, Stefan M. TI Prolonged Calorie Restriction Downregulates Skeletal Muscle mTORC1 Signaling Independent of Dietary Protein Intake and Associated microRNA Expression SO FRONTIERS IN PHYSIOLOGY LA English DT Article DE muscle protein content; energy deficit; rpS6; miR-99; miR-100 ID RESISTANCE EXERCISE; SHORT-TERM; TRANSPORTER EXPRESSION; HYPERTROPHY; REPRESSION; INGESTION; INCREASE; LEUCINE; TARGET; BONE AB Short-term (5-10 days) calorie restriction (CR) downregulates muscle protein synthesis, with consumption of a high protein-based diet attenuating this decline. Benefit of increase protein intake is believed to be due to maintenance of amino acid-mediated anabolic signaling through the mechanistic target of rapamycin complex 1 (mTORC1), however, there is limited evidence to support this contention. The purpose of this investigation was to determine the effects of prolonged CR and high protein diets on skeletal muscle mTORC1 signaling and expression of associated microRNA (miR). Twelve-week old male Sprague Dawley rats consumed ad libitum (AL) or calorie restricted (CR; 40%) adequate (10%, AIN-93M) or high (32%) protein milk-based diets for 16 weeks. Body composition was determined using dual energy X-ray absorptiometry and muscle protein content was calculated from muscle homogenate protein concentrations expressed relative to fat-free mass to estimate protein content. Western blot and RT-qPCR were used to determine mTORC1 signaling and mRNA and miR expression in fasted mixed gastrocnemius. Independent of dietary protein intake, muscle protein content was 38% lower (P < 0.05) in CR compared to AL. Phosphorylation and total Akt, mTOR, rpS6, and p70S6K were lower (P < 0.05) in CR vs. AL, and total rpS6 was associated with muscle protein content (r = 0.64, r(2) = 0.36). Skeletal muscle miR expression was not altered by either energy or protein intake. This study provides evidence that chronic CR attenuates muscle protein content by downregulating mTORC1 signaling. This response is independent of skeletal muscle miR and dietary protein. C1 [Margolis, Lee M.; Rivas, Donato A.; Berrone, Maria; Ezzyat, Yassine; Fielding, Roger A.] Tufts Univ, Jean Mayer Human Nutr Res Ctr Aging, USDA, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. [Margolis, Lee M.; Young, Andrew J.; McClung, James P.; Pasiakos, Stefan M.] US Army, Mil Nutr Div, Environm Med Res Inst, Natick, MA 01760 USA. RP Pasiakos, SM (reprint author), US Army, Mil Nutr Div, Environm Med Res Inst, Natick, MA 01760 USA. EM stefan.m.pasiakos.civ@mail.mil RI Pasiakos, Stefan/E-6295-2014; OI Pasiakos, Stefan/0000-0002-5378-5820; Rivas, Donato/0000-0002-4500-6233; , Lee/0000-0002-0652-1304 FU US Army Medical Research and Material Command; Dairy Research Institute; USDA [58-1950-4-003]; T32 NIDDK training grant [5T32DK062032-23]; NIA [KAG047247A-A1] FX This material is based on the work supported by the US Army Medical Research and Material Command, the Dairy Research Institute, and the USDA under agreement No. 58-1950-4-003. LM was supported by the T32 NIDDK training grant # 5T32DK062032-23 and DR was supported by the NIA K01 award # KAG047247A-A1. NR 35 TC 1 Z9 1 U1 9 U2 9 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-042X J9 FRONT PHYSIOL JI Front. Physiol. PD OCT 5 PY 2016 VL 7 AR 445 DI 10.3389/fphys.2016.00445 PG 11 WC Physiology SC Physiology GA DX9CD UT WOS:000384690000001 PM 27761114 ER PT J AU Sundaram, S Yan, L AF Sundaram, Sneha Yan, Lin TI Dietary energy restriction reduces high-fat diet-enhanced metastasis of Lewis lung carcinoma in mice SO ONCOTARGET LA English DT Article DE energy restriction; high-fat diet; Lewis lung carcinoma; metastasis; mice ID BREAST-CANCER; TUMOR-GROWTH; RADICAL PROSTATECTOMY; WEIGHT-LOSS; OBESITY; OVERWEIGHT; INFLAMMATION; RECURRENCE; EXERCISE; IMPACT AB The objective of this study was to determine whether a reduction in energy intake ameliorated the high-fat diet-enhanced spontaneous metastasis of Lewis lung carcinoma in mice. Male C57BL/6 mice were fed the AIN93G diet, a high-fat diet or a high-fat diet with a 5% restriction of the intake. Energy restriction reduced body adiposity and body weight, but maintained growth similar to mice fed the AIN93G diet. The high-fat diet significantly increased the number and size (cross-sectional area and volume) of metastases formed in lungs. Restricted feeding reduced the number of metastases by 23%, metastatic cross-sectional area by 32% and volume by 45% compared to the high-fat diet. The high-fat diet elevated plasma concentrations of proinflammatory cytokines (monocyte chemotactic protein-1, plasminogen activator inhibitor-1, leptin), angiogenic factors (vascular endothelial growth factor, tissue inhibitor of metalloproteinase-1) and insulin. Restricted feeding significantly reduced the high-fat diet-induced elevations in plasma concentrations of proinflammatory cytokines, angiogenic factors and insulin. These results demonstrated that a reduction in diet intake by 5% reduced high-fat diet-enhanced metastasis, which may be associated with the mitigation of adiposity and down-regulation of cancer-promoting proinflammatory cytokines and angiogenic factors. C1 [Sundaram, Sneha; Yan, Lin] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. RP Yan, L (reprint author), ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. EM lin.yan@ars.usda.gov FU U.S. Department of Agriculture, Agriculture Research Service [3062-51000-050-00D] FX Funding for this work was provided by the U.S. Department of Agriculture, Agriculture Research Service, Research Project 3062-51000-050-00D. NR 34 TC 0 Z9 0 U1 1 U2 1 PU IMPACT JOURNALS LLC PI ALBANY PA 6211 TIPTON HOUSE, STE 6, ALBANY, NY 12203 USA SN 1949-2553 J9 ONCOTARGET JI Oncotarget PD OCT 4 PY 2016 VL 7 IS 40 BP 65669 EP 65675 DI 10.18632/oncotarget.11598 PG 7 WC Oncology; Cell Biology SC Oncology; Cell Biology GA EB3QQ UT WOS:000387281000087 PM 27582541 ER PT J AU Lupolova, N Dallman, TJ Matthews, L Bono, JL Gally, DL AF Lupolova, Nadejda Dallman, Timothy J. Matthews, Louise Bono, James L. Gally, David L. TI Support vector machine applied to predict the zoonotic potential of E-coli O157 cattle isolates SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE machine learning; zoonosis; Shiga toxin; E. coli; cattle ID HEMOLYTIC-UREMIC SYNDROME; PREVALENCE; SEROGROUPS; VIRULENCE; OUTBREAKS; SCOTLAND; O145; O111; O103; O26 AB Sequence analyses of pathogen genomes facilitate the tracking of disease outbreaks and allow relationships between strains to be reconstructed and virulence factors to be identified. However, these methods are generally used after an outbreak has happened. Here, we show that support vector machine analysis of bovine E. coli O157 isolate sequences can be applied to predict their zoonotic potential, identifying cattle strains more likely to be a serious threat to human health. Notably, only a minor subset (less than 10%) of bovine E. coli O157 isolates analyzed in our datasets were predicted to have the potential to cause human disease; this is despite the fact that the majority are within previously defined pathogenic lineages I or I/II and encode key virulence factors. The predictive capacity was retained when tested across datasets. The major differences between human and bovine E. coli O157 isolates were due to the relative abundances of hundreds of predicted prophage proteins. This finding has profound implications for public health management of disease because interventions in cattle, such a vaccination, can be targeted at herds carrying strains of high zoonotic potential. Machine-learning approaches should be applied broadly to further our understanding of pathogen biology. C1 [Lupolova, Nadejda; Gally, David L.] Univ Edinburgh, Roslin Inst, Div Immun & Infect, Roslin EH25 9RG, Midlothian, Scotland. [Lupolova, Nadejda; Gally, David L.] Univ Edinburgh, Royal Dick Sch Vet Studies, Roslin EH25 9RG, Midlothian, Scotland. [Dallman, Timothy J.] Natl Infect Serv, Publ Hlth England, London NW9 5EQ, England. [Matthews, Louise] Univ Glasgow, Coll Med Vet & Life Sci, Inst Biodivers Anim Hlth & Comparat Med, Glasgow G12 8QQ, Lanark, Scotland. [Bono, James L.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Gally, DL (reprint author), Univ Edinburgh, Roslin Inst, Div Immun & Infect, Roslin EH25 9RG, Midlothian, Scotland.; Gally, DL (reprint author), Univ Edinburgh, Royal Dick Sch Vet Studies, Roslin EH25 9RG, Midlothian, Scotland. EM dgally@ed.ac.uk OI Matthews, Louise/0000-0003-4420-8367 FU Food Standards Scotland and the Food Standards Agency Grant [FS101055]; University of Edinburgh studentship; core Biotechnology and Biological Sciences Research Council strategic programme [BB/J004227/1]; National Institute for Health Research Health Protection Research Unit in Gastrointestinal Infections at the University of Liverpool; Public Health England; University of East Anglia; University of Oxford; Institute of Food Research FX We would like to acknowledge the value of human and bovine E. coli O157 sequence data available from previous published studies, especially work from the Wellcome Trust IPRAVE consortium, Public Health England, and the Scottish E. coli reference laboratory. This work was supported by Food Standards Scotland and the Food Standards Agency Grant FS101055 (to D.L.G., T.J.D., and L.M.), which has allowed the continuation of significant EHEC O157 research in the UK. This research was also supported by a University of Edinburgh studentship (N.L.) and core Biotechnology and Biological Sciences Research Council strategic programme Grant BB/J004227/1 (to D.L.G.). T.J.D. was funded by the National Institute for Health Research Health Protection Research Unit in Gastrointestinal Infections at the University of Liverpool in partnership with Public Health England, University of East Anglia, University of Oxford, and the Institute of Food Research. NR 30 TC 2 Z9 2 U1 9 U2 9 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD OCT 4 PY 2016 VL 113 IS 40 BP 11312 EP 11317 DI 10.1073/pnas.1606567113 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DX6XI UT WOS:000384528900075 PM 27647883 ER PT J AU Yahia, N Brown, CA Rapley, M Chung, M AF Yahia, Najat Brown, Carrie A. Rapley, Melyssa Chung, Mei TI Level of nutrition knowledge and its association with fat consumption among college students SO BMC PUBLIC HEALTH LA English DT Article DE Nutrition education; Fat consumption; Nutritional knowledge; University students ID AMERICAN-HEART-ASSOCIATION; UNIVERSITY-STUDENTS; GENDER-DIFFERENCES; PHYSICAL-ACTIVITY; FOOD CHOICE; BODY-WEIGHT; DISEASE; STROKE; FRUIT; PERCEPTION AB Background: Intake of saturated fat, trans fat, and cholesterol has been associated with increased risk of coronary heart disease. The aim of this study was to explore whether increased nutrition knowledge is associated with a reduction in the consumption of unhealthy fats in a sample of university students. Methods: A sample of 231 students, with a mean age of 20 years, was recruited from university campus during spring 2012. Students completed a validated questionnaire related to students' demographic, nutrition knowledge, and daily fat consumption. Weight, height, and waist circumference were measured. Data were analyzed using one-way ANOVA, chi-square, and student's t-test. Results: Results indicate that female students have greater nutrition knowledge than male students (the mean nutrition score for women was 5 points higher than that of men (P = 0.01)). Nutrition knowledge was negatively correlated with fat and cholesterol intake. Students who consumed more than 35 % calories from fat or >300 mg of cholesterol daily had lower mean nutrition scores than those students with lower fat or cholesterol intake (8 points lower and 7.9 points lower, respectively). Using linear regression for nutrition scores on estimated saturated fat intake and cholesterol intake (controlling for gender, height, weight, age, and dieting), nutrition scores were negatively associated with saturated fat intake (-0.15, P <0.0001) and cholesterol intake (-1.38, P <0.0001). Conclusion: Students with greater nutritional knowledge consumed less unhealthy fats and cholesterol. This finding magnifies the role of nutrition education as a potential tool in health campaigns to promote healthy eating patterns among college students. Results of this pilot study can inform the design of future nutrition education intervention studies to assess the efficacy of nutrition knowledge on pattern of fat consumption among college students. C1 [Yahia, Najat; Rapley, Melyssa] Cent Michigan Univ, Dept Human Environm Studies, Wightman 108, Mt Pleasant, MI 48859 USA. [Brown, Carrie A.] Tufts Univ, Jean Mayer USDA Human Nutr Ctr Aging, Boston, MA 02111 USA. [Chung, Mei] Tufts Univ, Dept Publ Hlth & Family Med, Sch Med, 136 Harrison Ave,Jaharis 264, Boston, MA 02111 USA. [Brown, Carrie A.] Boston Univ, Dept Biostat, 801 Massachusetts Ave, Boston, MA 02118 USA. RP Yahia, N (reprint author), Cent Michigan Univ, Dept Human Environm Studies, Wightman 108, Mt Pleasant, MI 48859 USA. EM yahia1n@cmich.edu FU Faculty Research and Creative Endeavors (FRCE) Premier Display grant at CMU FX This work was partially supported by the Faculty Research and Creative Endeavors (FRCE) Premier Display grant at CMU. NR 54 TC 0 Z9 0 U1 35 U2 35 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2458 J9 BMC PUBLIC HEALTH JI BMC Public Health PD OCT 4 PY 2016 VL 16 AR 1047 DI 10.1186/s12889-016-3728-z PG 10 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA DX9FW UT WOS:000384700000003 PM 27716127 ER PT J AU Sharma, T Dreyer, I Kochian, L Pineros, MA AF Sharma, Tripti Dreyer, Ingo Kochian, Leon Pineros, Miguel A. TI The ALMT Family of Organic Acid Transporters in Plants and Their Involvement in Detoxification and Nutrient Security SO FRONTIERS IN PLANT SCIENCE LA English DT Review DE anion channel; ALMT; aluminum tolerance; nutrient transport; malate transport; citrate transport; review ID ACTIVATED MALATE TRANSPORTER; DEPENDENT ANION CHANNEL; WHEAT TRITICUM-AESTIVUM; ARABIDOPSIS HYPOCOTYL CELLS; ALUMINUM TOLERANCE; GUARD-CELLS; PLASMA-MEMBRANE; MALIC-ACID; STOMATAL MOVEMENT; CHLORIDE CHANNELS AB About a decade ago, members of a new protein family of anion channels were discovered on the basis of their ability to confer on plants the tolerance toward toxic aluminum ions in the soil. The efflux of Al3+-chelating malate anions through these channels is stimulated by external Al3+ ions. This feature of a few proteins determined the name of the entire protein family as Aluminum-activated Malate Transporters (ALMT). Meanwhile, after several years of research, it is known that the physiological roles of ALMTs go far beyond Al-detoxification. In this review article we summarize the current knowledge on this transporter family and assess their involvement in diverse physiological processes. C1 [Sharma, Tripti; Dreyer, Ingo] Univ Talca, Ctr Bioinformat & Simulac Mol, Fac Ingn, Talca, Chile. [Kochian, Leon; Pineros, Miguel A.] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Dreyer, I (reprint author), Univ Talca, Ctr Bioinformat & Simulac Mol, Fac Ingn, Talca, Chile.; Pineros, MA (reprint author), Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM idreyer@utalca.cl; map25@cornell.edu RI Dreyer, Ingo/N-4747-2014; OI Pineros, Miguel/0000-0002-7166-1848 FU FONDECYT of the Comision Nacional Cientifica y Tecnologica of Chile [1150054]; National Science Foundation [NSF/IOS-1444435] FX This work was supported by the FONDECYT grant No 1150054 of the Comision Nacional Cientifica y Tecnologica of Chile (ID and TS) and National Science Foundation award NSF/IOS-1444435 (MP and LK). NR 89 TC 1 Z9 2 U1 26 U2 26 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD OCT 4 PY 2016 VL 7 AR 1488 DI 10.3389/fpls.2016.01488 PG 12 WC Plant Sciences SC Plant Sciences GA DX9CW UT WOS:000384692200001 PM 27757118 ER PT J AU Weller, TJ Castle, KT Liechti, F Hein, CD Schirmacher, MR Cryan, PM AF Weller, Theodore J. Castle, Kevin T. Liechti, Felix Hein, Cris D. Schirmacher, Michael R. Cryan, Paul M. TI First Direct Evidence of Long-distance Seasonal Movements and Hibernation in a Migratory Bat SO SCIENTIFIC REPORTS LA English DT Article ID TORPOR; ENERGETICS; TELEMETRY; LASIURUS; SONGBIRD; TAGS AB Understanding of migration in small bats has been constrained by limitations of techniques that were labor-intensive, provided coarse levels of resolution, or were limited to population-level inferences. Knowledge of movements and behaviors of individual bats have been unknowable because of limitations in size of tracking devices and methods to attach them for long periods. We used sutures to attach miniature global positioning system (GPS) tags and data loggers that recorded light levels, activity, and temperature to male hoary bats (Lasiurus cinereus). Results from recovered GPS tags illustrated profound differences among movement patterns by individuals, including one that completed a > 1000 km round-trip journey during October 2014. Data loggers allowed us to record sub-hourly patterns of activity and torpor use, in one case over a period of 224 days that spanned an entire winter. In this latter bat, we documented 5 torpor bouts that lasted >= 16 days and a flightless period that lasted 40 nights. These first uses of miniature tags on small bats allowed us to discover that male hoary bats can make multi-directional movements during the migratory season and sometimes hibernate for an entire winter. C1 [Weller, Theodore J.] US Forest Serv, USDA, Pacific Southwest Res Stn, 1700 Bayview Dr, Arcata, CA 95521 USA. [Castle, Kevin T.] Wildlife Vet Consulting, 840 Sundance Dr, Livermore, CO 80536 USA. [Liechti, Felix] Swiss Ornithol Inst, Seerose 1, CH-6204 Sempach, Switzerland. [Hein, Cris D.; Schirmacher, Michael R.] Bat Conservat Int, POB 162603, Austin, TX 78716 USA. [Cryan, Paul M.] US Geol Survey, Ft Collins Sci Ctr, 2150 Ctr Ave,Bldg C, Ft Collins, CO 80526 USA. RP Weller, TJ (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, 1700 Bayview Dr, Arcata, CA 95521 USA. EM tweller@fs.fed.us OI Cryan, Paul/0000-0002-2915-8894 FU USDA Forest Service Pacific Southwest Research Station; U.S. Geological Survey, Fort Collins Science Center FX Funding for this project was provided by the USDA Forest Service Pacific Southwest Research Station and U.S. Geological Survey, Fort Collins Science Center. We are grateful to the California State Parks, North Coast Redwood District for granting us access and permission to conduct this study. We thank A. Brokaw, J. Clerc, T. Dewey, B. Fahey, M. Lau, C. Long, M. McKenzie, S. Mendia, M. Parker, K. Southall, V. Stover, J. Szewczak, and C. Zurek for invaluable assistance in the field. J. Baldwin prepared the figure on arousal times. Special thanks to R. Diehl for sacrificing his chance to deploy experimental data loggers on birds and instead contributing them to advancement of knowledge regarding the other class of extant flying vertebrates. Comments from B. Zielinski and J. Wolfe improved the manuscript. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 54 TC 0 Z9 0 U1 24 U2 24 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD OCT 4 PY 2016 VL 6 AR 34585 DI 10.1038/srep34585 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DX7DI UT WOS:000384546200001 PM 27698492 ER PT J AU Sainju, UM Lenssen, AW Allen, BL Stevens, WB Jabro, JD AF Sainju, Upendra M. Lenssen, Andrew W. Allen, Brett L. Stevens, William B. Jabro, Jalal D. TI Nitrogen balance in response to dryland crop rotations and cultural practices SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Cropping system; Management practice; Nitrogen input; Nitrogen output; Nitrogen budget; Soil total nitrogen ID SOIL-NITROGEN; LIVESTOCK SYSTEMS; SEMIARID MONTANA; N-FERTILIZATION; FOOD-PRODUCTION; TILLAGE; SEQUENCE; AGROECOSYSTEMS; LOSSES; CARBON AB Nitrogen balance provides a measure of agroecosystem performance and environmental sustainability by taking into accounts of N inputs and outputs and N retention in the soil. The objective of this study was to evaluate N balance based on N inputs and outputs and soil N sequestration after 7 yr in response to five dryland crop rotations (two 4-yr stacked and two 4-yr alternate-year rotations and one monocropping) and two cultural practices arranged in a split-plot design in the northern Great Plains, USA. Stacked rotations were durum (Triticum turgidum L.)-durum-canola (Brassica napus L)-pea (Pisum sativum L) (D-D-C-P) and durum-durum-flax (Linum usitadssimum L.)-pea (D-D-F-P). Alternate-year rotations were durum-canola-durum-pea (D-C-D-P) and durum-flax-durum-pea (D-F-D-P). Monocroppping was continuous durum (CD). Cultural practices were traditional (conventional till, recommended seed rate, broadcast N fertilization, and reduced stubble height) and ecological (no-till, increased seed rate, banded N fertilization, and increased stubble height). Total annual N input due to N fertilization, pea N fixation, atmospheric N deposition, crop seed N, and nonsymbiotic N fixation was lower in CD than other crop rotations, regardless of cultural practices. Total N output due to crop grain N removal and N losses due to denitrification, volatilization, plant senescence, N leaching, gaseous N (NO.) emissions, and surface runoff was lower in traditional CD and D-F-D-P than traditional D-C-D-P and ecological D-C-D-P, D-D-C-P, and D-F-D-P. Nitrogen sequestration rate at 0-125 cm from 2005 to 2011 ranged from 40 kg N ha(-1) yr(-1) for ecological D-D-F-P to 52 kg N ha(-1) yr(-1) for ecological CD. Nitrogen balance ranged from -39 to -36 kg N ha(-1) yr(-1) with CD compared to 9-25 kg N ha(-1) yr(-1) with other crop rotations in both cultural practices. Because of reduced reliance on external N inputs and increased grain N removal, N flow, and N surplus, crop rotations with legumes, nonlegumes, and oilseed crops in the rotation can be productive and environmentally sustainable compared with monocropping, regardless of cultural practices. Published by Elsevier B.V. C1 [Sainju, Upendra M.; Allen, Brett L.; Stevens, William B.; Jabro, Jalal D.] USDA ARS, Northern Plains Agr Res Lab, 1500 North Cent Ave, Sidney, MT 59270 USA. [Lenssen, Andrew W.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. RP Sainju, UM (reprint author), USDA ARS, Northern Plains Agr Res Lab, 1500 North Cent Ave, Sidney, MT 59270 USA. EM upendra.sainju@ars.usda.gov OI Sainju, Upendra/0000-0001-6943-733X NR 38 TC 0 Z9 0 U1 14 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD OCT 3 PY 2016 VL 233 BP 25 EP 32 DI 10.1016/j.agee.2016.08.023 PG 8 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA ED7YK UT WOS:000389088400003 ER PT J AU Ashworth, AJ Weiss, SA Keyser, PD Allen, FL Tyler, DD Taylor, A Beamer, KP West, CP Pote, DH AF Ashworth, A. J. Weiss, S. A. Keyser, P. D. Allen, F. L. Tyler, D. D. Taylor, A. Beamer, K. P. West, C. P. Pote, D. H. TI Switchgrass composition and yield response to alternative soil amendments under intensified heat and drought conditions SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Review DE Biochar; Legume intercropping; Alternative soil amendments; Climatic intensification; Feedstock composition; Plant adaptation ID CROPPING SYSTEMS; UNITED-STATES; COVER CROPS; BIOCHAR; MANAGEMENT; FEEDSTOCK; DYNAMICS AB Switchgrass (Panicum virgatum L.) and guinea grass (Panicum maximum Jacq.) have been proposed as sustainable alternatives to fossil fuels in subtropical and tropical environments, respectively; although still requiring non-renewable inputs, notably, fertilizer-nitrogen (N). Furthermore, climatic intensification forecasts suggest southeastern USA may emulate more tropical or subtropical growing conditions resulting in altered N dynamics and plant physiology. Objectives were to determine: (i) effects of biochar (1 and 2 Mg ha(-1)), two intercropped legumes [sunn hemp (Crotaiaria juncea L.) and pigeon pea (Cajanus cajan L) intercrops] versus inorganic N [67 kg ha-land 0 kg ha(-1)] on feedstock and soil characteristics and biomass yield; (ii) how feedstock composition is affected over three harvest dates; and, (iii) switchgrass adaptation to more extreme (tropical) growing conditions. For both species, yield and feedstock composition were influenced by harvest timing (P < 0.05), whereas soil amendments influenced composition to a lesser extent over the duration of this study (P > 0.05). In general, initial harvests had greater digestible 5- and 6-carbon sugars and N, P, and K tissue levels, whereas in subsequent harvests, higher acid- and neutral-detergent fiber levels were observed, suggesting lesser potential fermentability. Desired feedstock characteristics can therefore be manipulated by harvest timing. Yield results suggest pigeon pea and sunn hemp intercrops, and biochar (1 Mg ha(-1)) may result in equivalent yields as N fertilizer per harvest (P < 0.05). However, the 2 Mg ha(-1) biochar rate in the tropics adversely impacted yields, perhaps due to N immobilization by biochar. Switchgrass adaptation and competitiveness was moderate (5-30% weed cover) under an intensified climate. Growth can therefore be maintained under a stochastic climate due to its C-4 pathway and competitive growth on marginal soils. Published by Elsevier B.V. C1 [Ashworth, A. J.; Pote, D. H.] USDA ARS, Bumpers Small Farms Res Ctr, 6883 S Hwy 23, Booneville, AR 72927 USA. [Allen, F. L.] Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA. [Keyser, P. D.] Univ Tennessee, Dept Forestry Wildlife & Fisheries, Knoxville, TN 37996 USA. [Weiss, S. A.; Beamer, K. P.] Univ Virgin Isl, Agr Expt Stn, St Croix, VI 00850 USA. [Tyler, D. D.] Univ Tennessee, Biosyst Engn & Soil Sci, Knoxville, TN 37996 USA. [Taylor, A.] Univ Tennessee, Ctr Renewable Carbon, Knoxville, TN 37996 USA. [West, C. P.] Texas Tech Univ, Dept Plant & Soil Sci, Lubbock, TX 79410 USA. RP Ashworth, AJ (reprint author), USDA ARS, Bumpers Small Farms Res Ctr, 6883 S Hwy 23, Booneville, AR 72927 USA. EM Amanda.Ashworth@ars.usda.gov OI Ashworth, Amanda/0000-0002-3218-8939 FU Southeastern Sun Grant Center; U.S. Department of Transportation Research and Innovative Technology Administration [DTOS59-07-G-00050] FX Support for this research was provided by a grant from the Southeastern Sun Grant Center with funds provided by the U.S. Department of Transportation Research and Innovative Technology Administration (DTOS59-07-G-00050). In addition, authors thank the Agricultural Research and Education Centers in St. Croix, USVI and in Greenville, TN (Rob Ellis) and Knoxville, TN (Lee Ellis) for their help in collecting data and making this research possible. Lastly, gratitude is extended to the laboratory of Dr. Samy Sadaka at the University of Arkansas for the production of biochars used in field investigations. NR 36 TC 0 Z9 0 U1 5 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD OCT 3 PY 2016 VL 233 BP 415 EP 424 DI 10.1016/j.agee.2016.09.041 PG 10 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA ED7YK UT WOS:000389088400044 ER PT J AU Cobb, AB Wilson, GWT Goad, CL Bean, SR Kaufman, RC Herald, TJ Wilson, JD AF Cobb, Adam B. Wilson, Gail W. T. Goad, Carla L. Bean, Scott R. Kaufman, Rhett C. Herald, Thomas J. Wilson, Jeff D. TI The role of arbuscular mycorrhizal fungi in grain production and nutrition of sorghum genotypes: Enhancing sustainability through plant-microbial partnership SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Mycorrhizal fungi; Sorghum genotypes; Grain nutrition ID TALLGRASS PRAIRIE; WHEAT CULTIVARS; PHOSPHORUS; COLONIZATION; DOMESTICATION; COMMUNITIES; SYSTEMS; BICOLOR; STARCH AB The responsiveness of 3 landrace (open-pollinated) sorghum cultivars and 3 modern (commercial) hybrids to arbuscular mycorrhizal (AM) fungi and commercial fertilizers were assessed. Sorghum genotypes were selected to represent a range of sorghum breeding development. This comparison was conducted to link grain production and quality with crop nutrition strategies (AM symbiosis versus fertility amendments). The open-pollinated cultivars produced an average of 206% more vegetative biomass and 285% more grain per plant, compared to commercial hybrids when grown with AM fungi and no fertilization. Furthermore, the average protein production of open-pollinated cultivars was 320% greater, compared to hybrids grown under the same low-fertility conditions. Percent AM root colonization was 149% greater in open-pollinated cultivars compared to hybrids, and across all genotypes, AM colonization was significantly correlated with total grain mineral content. Sorghum cultivars were significantly more responsive to mycorrhizal symbiosis than commercial hybrids for nutrient uptake and subsequent grain production and quality, while hybrids were significantly more responsive to fertilization. (C) 2016 Elsevier B.V. All rights reserved. C1 [Cobb, Adam B.; Wilson, Gail W. T.; Goad, Carla L.] Oklahoma State Univ, 008C AGH, Stillwater, OK 74078 USA. [Bean, Scott R.; Kaufman, Rhett C.; Herald, Thomas J.; Wilson, Jeff D.] USDA ARS, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. [Kaufman, Rhett C.] Bayer CropSci, 925 Cty Rd 378, Beaver Crossing, NE 68313 USA. RP Cobb, AB (reprint author), Oklahoma State Univ, 008C AGH, Stillwater, OK 74078 USA. EM abcobb@okstate.edu; gail.wilson@okstate.edu; carla.goad@okstate.edu; scott.bean@ars.usda.gov; rhett.kaufman@bayer.com; tom.herald@ars.usda.gov; jeff.d.wilson@ars.usda.gov FU Oklahoma State University, Department of Natural Resource Ecology and Management; Oklahoma Agriculture Experiment Station, Stillwater, Oklahoma, USA; USDA-ARS, Center for Grain and Animal Health Research, Manhattan, Kansas, USA FX This work was supported by Oklahoma State University, Department of Natural Resource Ecology and Management, and the Oklahoma Agriculture Experiment Station, Stillwater, Oklahoma, USA. Partial support received by USDA-ARS, Center for Grain and Animal Health Research, Manhattan, Kansas, USA. USDA collaborators conducted laboratory analysis, and interpretation. OSU collaborators were responsible for conducting the study, data collection, writing, and statistical analysis. All collaborators were involved in study design and editorial review of the article. NR 51 TC 0 Z9 0 U1 21 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD OCT 3 PY 2016 VL 233 BP 432 EP 440 DI 10.1016/j.agee.2016.09.024 PG 9 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA ED7YK UT WOS:000389088400046 ER PT J AU Haley, BJ Kim, SW Pettengill, J Luo, Y Karns, JS Van Kessel, JAS AF Haley, Bradd J. Kim, Seon Woo Pettengill, James Luo, Yan Karns, Jeffrey S. Van Kessel, Jo Ann S. TI Genomic and Evolutionary Analysis of Two Salmonella enterica Serovar Kentucky Sequence Types Isolated from Bovine and Poultry Sources in North America SO PLOS ONE LA English DT Article ID FIELD GEL-ELECTROPHORESIS; ANTIMICROBIAL RESISTANCE; POPULATION-STRUCTURE; EFFECTOR PROTEINS; VIBRIO-CHOLERAE; HEMOLYSIN-III; DAIRY FARM; SEROTYPES; TYPHIMURIUM; PREVALENCE AB Salmonella enterica subsp. enterica serovar Kentucky is frequently isolated from healthy poultry and dairy cows and is occasionally isolated from people with clinical disease. A genomic analysis of 119 isolates collected in the United States from dairy cows, ground beef, poultry and poultry products, and human clinical cases was conducted. Results of the analysis demonstrated that the majority of poultry and bovine-associated S. Kentucky were sequence type (ST) 152. Several bovine-associated (n = 3) and food product isolates (n = 3) collected from the United States and the majority of human clinical isolates were ST198, a sequence type that is frequently isolated from poultry and occasionally from human clinical cases in Northern Africa, Europe and Southeast Asia. A phylogenetic analysis indicated that both STs are more closely related to other Salmonella serovars than they are to each other. Additionally, there was strong evidence of an evolutionary divergence between the poultry-associated and bovine-associated ST152 isolates that was due to polymorphisms in four core genome genes. The ST198 isolates recovered from dairy farms in the United States were phylogenetically distinct from those collected from human clinical cases with 66 core genome SNPs differentiating the two groups, but more isolates are needed to determine the significance of this distinction. Identification of S. Kentucky ST198 from dairy animals in the United States suggests that the presence of this pathogen should be monitored in food-producing animals. C1 [Haley, Bradd J.; Kim, Seon Woo; Karns, Jeffrey S.; Van Kessel, Jo Ann S.] ARS, Environm Microbial & Food Safety Lab, Beltsville Area Res Ctr, USDA, Beltsville, MD 20705 USA. [Pettengill, James; Luo, Yan] US FDA, Off Analyt & Outreach, Ctr Food Safety & Appl Nutr, College Pk, MD USA. RP Van Kessel, JAS (reprint author), ARS, Environm Microbial & Food Safety Lab, Beltsville Area Res Ctr, USDA, Beltsville, MD 20705 USA. EM joann.vankessel@ars.usda.gov FU internal USDA/ARS research funding FX This project was supported by internal USDA/ARS research funding.; We gratefully acknowledge Jakeitha Sonnier for laboratory assistance. This project was supported by internal USDA/ARS research funding. The mention of a trade name, proprietary product, or specific equipment does not constitute guarantee or warranty by the USDA and does not imply approval to the exclusion of other products that might be suitable. NR 62 TC 0 Z9 0 U1 1 U2 1 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD OCT 3 PY 2016 VL 11 IS 10 AR e0161225 DI 10.1371/journal.pone.0161225 PG 36 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ0TR UT WOS:000385553100008 PM 27695032 ER PT J AU Li, W Katin-Grazzini, L Krishnan, S Thammina, C El-Tanbouly, R Yer, H Merewitz, E Guillard, K Inguagiato, J McAvoy, RJ Liu, ZR Li, Y AF Li, Wei Katin-Grazzini, Lorenzo Krishnan, Sanalkumar Thammina, Chandra El-Tanbouly, Rania Yer, Huseyin Merewitz, Emily Guillard, Karl Inguagiato, John McAvoy, Richard J. Liu, Zongrang Li, Yi TI A Novel Two-Step Method for Screening Shade Tolerant Mutant Plants via Dwarfism SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE mutation breeding; screening method; dwarfism; gibberellins; shade tolerance ID PERENNIAL RYEGRASS; TRINEXAPAC-ETHYL; TALL FESCUE; LIGHT; BERMUDAGRASS; RESPONSES; GROWTH; TURFGRASS; DROUGHT; STRESS AB When subjected to shade, plants undergo rapid shoot elongation, which often makes them more prone to disease and mechanical damage. Shade-tolerant plants can be difficult to breed; however, they offer a substantial benefit over other varieties in low light areas. Although perennial ryegrass (Lolium perenne L.) is a popular species of turf grasses because of their good appearance and fast establishment, the plant normally does not perform well under shade conditions. It has been reported that, in turfgrass, induced dwarfism can enhance shade tolerance. Here we describe a two-step procedure for isolating shade tolerant mutants of perennial ryegrass by first screening for dominant dwarf mutants, and then screening dwarf plants for shade tolerance. The two-step screening process to isolate shade tolerant mutants can be done efficiently with limited space at early seedling stages, which enables quick and efficient isolation of shade tolerant mutants, and thus facilitates development of shade tolerant new cultivars of turfgrasses. Using the method, we isolated 136 dwarf mutants from 300,000 mutagenized seeds, with 65 being shade tolerant (0.022%). When screened directly for shade tolerance, we recovered only four mutants from a population of 150,000 (0.003%) mutagenized seeds. One shade tolerant mutant, shadow-1, was characterized in detail. In addition to dwarfism, shadow-1 and its sexual progeny displayed high degrees of tolerance to both natural and artificial shade. We showed that endogenous gibberellin (GA) content in shadow-1 was higher than wild-type controls, and shadow-1 was also partially GA insensitive. Our novel, simple and effective two-step screening method should be applicable to breeding shade tolerant cultivars of turfgrasses, ground covers, and other economically important crop plants that can be used under canopies of existing vegetation to increase productivity per unit area of land. C1 [Li, Wei; Katin-Grazzini, Lorenzo; Thammina, Chandra; El-Tanbouly, Rania; Yer, Huseyin; Guillard, Karl; Inguagiato, John; McAvoy, Richard J.; Li, Yi] Univ Connecticut, Dept Plant Sci & Landscape Architecture, Storrs, CT 06269 USA. [Krishnan, Sanalkumar; Merewitz, Emily] Michigan State Univ, Dept Crop Sci, E Lansing, MI 48824 USA. [El-Tanbouly, Rania] Univ Alexandria, Fac Agr, Dept Floriculture Omamental Hort & Landscape Gard, Alexandria, Egypt. [Liu, Zongrang] ARS, Appalachian Fruit Res Stn, USDA, Kearneysville, WV USA. RP Li, Y (reprint author), Univ Connecticut, Dept Plant Sci & Landscape Architecture, Storrs, CT 06269 USA. EM yi.li@uconn.edu FU Storrs Agricultural Experiment Station FX We thank the personnel at the University of Massachusetts for their kind assistance in gamma-ray irradiation of seed materials. We also thank Mr. Steve Olsen and his crew at the University of Connecticut, Plant Science Research and Education Facility for their help in growing and maintaining mutant plant populations. This project is financially supported by the Storrs Agricultural Experiment Station to YL. NR 40 TC 0 Z9 0 U1 16 U2 16 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD OCT 3 PY 2016 VL 7 AR 1495 DI 10.3389/fpls.2016.01495 PG 12 WC Plant Sciences SC Plant Sciences GA DX6OV UT WOS:000384503900001 PM 27752260 ER PT J AU Nyarko, E Kniel, KE Millner, PD Luo, YG Handy, ET Reynnells, R East, C Sharma, M AF Nyarko, Esmond Kniel, Kalmia E. Millner, Patricia D. Luo, Yaguang Handy, Eric T. Reynnells, Russell East, Cheryl Sharma, Manan TI Survival and growth of Listeria monocytogenes on whole cantaloupes is dependent on site of contamination and storage temperature SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Listeria monocytogenes; Listeriosis; United States (US); Cantaloupes; Multistate; Outbreak ID FRESH-CUT CANTALOUPE; UNITED-STATES; MULTISTATE OUTBREAK; PREVENTION; SURFACES; QUALITY; PIECES; RIND AB Whole cantaloupes (Cucumis melo L.), marketed as 'Rocky Ford', were implicated in a large multi-state outbreak of listeriosis in the United States in 2011; however, survival and growth of Listeria monocytogenes on whole cantaloupes remains relatively unexplored. The research presented here evaluated three different storage temperatures, two sites of contamination of cantaloupes, and two cantaloupe varieties to determine their effect on the survival of L. monocytogenes. 'Athena' and 'Rocky Ford' cantaloupe cultivars were grown in soil and harvested, and individual melons subsequently received a multi-strain inoculum of L. monocytogenes (6 log CFU/melon), which were then stored at 4 degrees C, 10 degrees C, and 25 degrees C. Changes in L monocytogenes populations on the rinds and stem scars of cantaloupes stored at each temperature were determined at selected times for up to 15 days. An analysis of variance revealed that inoculation site and storage temperature significantly affected survival of L monocytogenes on cantaloupes during storage (p < 0.05), but cultivar did not influence L monocytogenes (p > 0.05). Populations of L monocytogenes on stem scars of cantaloupes stored at 25 degrees C increased by 1-2 log CFU/melon on day 1, and were significantly greater than those on cantaloupes stored at 4 degrees C or 10 degrees C (p < 0.05), which remained constant or increased by approximately 03 log CFU/melon, respectively, over the same time period. A decrease of 2-5 log CFU/melon of L. monocytogenes occurred on the rinds of cantaloupes during storage by day 7, and were not significantly different at the three different storage temperatures (p > 0.05). In trials performed in rind juice extracts, populations of L. monocytogenes decreased by 3 log CFU/mL when stored at 25 degrees C by day 3, but grew by 3-4 log CFU/mL when stored at 4 degrees C over 7 days. Overall, site of contamination and storage temperature influenced the survival of L. monocytogenes on cantaloupes more than cantaloupe cultivar type. Published by Elsevier B.V. C1 [Nyarko, Esmond; Kniel, Kalmia E.] Univ Delaware, Dept Anim & Food Sci, 044 Townsend Hall 531 S Coll Ave, Newark, DE 19716 USA. [Millner, Patricia D.; Luo, Yaguang; Handy, Eric T.; Reynnells, Russell; East, Cheryl; Sharma, Manan] United States Dept Agr, Agr Res Serv, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. RP Sharma, M (reprint author), United States Dept Agr, Agr Res Serv, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. EM esmond.nyarko@ars.usda.gov; kniel@udel.edu; pat.millner@ars.usda.gov; yaguang.luo@ars.usda.gov; eric.handy@ars.usda.gov; cheryl.east@ars.usda.gov; manan.sharma@ars.usda.gov FU FDA [224-11-2046]; USDA ARS [224-11-2046, 58-1245-4-111, 8042-32420-005-00]; University of Delaware [58-1245-4-111] FX The authors would like to thank Patrick Spanninger and Dr. Gordon Johnson at the University of Delaware, Newark and Georgetown, Delaware; Kate White, Dave Clark, and Richard Stonebraker at the Environmental Microbial and Food Safety Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, Maryland; and Yi Chen, Dumitru Macarisin, Michael Mahovic, Annemarie Buchholz, and Mickey Parish at the Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration (U.S. FDA) for their assistance with this study. This study was funded by Inter-Agency Agreement 224-11-2046 between FDA and USDA ARS, Specific Cooperative Agreement 58-1245-4-111 between USDA ARS and University of Delaware, and USDA ARS Project number 8042-32420-005-00 "Microbial Ecology and Safety of Fresh Produce". NR 22 TC 2 Z9 2 U1 10 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD OCT 3 PY 2016 VL 234 BP 65 EP 70 DI 10.1016/j.ijfoodmicro.2016.06.030 PG 6 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA DV0IE UT WOS:000382600400009 PM 27376678 ER PT J AU Sabo, R Yermakov, A Law, CT Elhajjar, R AF Sabo, Ronald Yermakov, Aleksey Law, Chiu Tai Elhajjar, Rani TI Nanocellulose-Enabled Electronics, Energy Harvesting Devices, Smart Materials and Sensors: A Review SO JOURNAL OF RENEWABLE MATERIALS LA English DT Review DE Cellulose nanofibrils; cellulose nanocrystals; flexible electronics; photovoltaics; batteries; magnetostrictive composites ID OPTICALLY TRANSPARENT COMPOSITES; LI-ION BATTERIES; BACTERIAL CELLULOSE; THIN-FILMS; TERFENOL-D; MICROFIBRILLATED CELLULOSE; CARBON NANOTUBE; MAGNETOSTRICTIVE ACTUATORS; BIOMEDICAL APPLICATIONS; FLEXIBLE ELECTRONICS AB Cellulose nanomaterials have a number of interesting and unique properties that make them well-suited for use in electronics applications such as energy harvesting devices, actuators and sensors. Cellulose nanofibrils and nanocrystals have good mechanical properties, high transparency, and low coefficient of thermal expansion, among other properties that facilitate both active and inactive roles in electronics and related devices. For example, these nanomaterials have been demonstrated to operate as substrates for flexible electronics and displays, to improve the efficiency of photovoltaics, to work as a component of magnetostrictive composites and to act as a suitable lithium ion battery separator membrane. A discussion and overview of additional potential applications and of previously published research using cellulose nanomaterials for these advanced applications is provided in this article. The concept of using cellulose nanofibrils in stimuli-responsive materials is illustrated with highlights of preliminary results from magnetostrictive nanocellulose membranes actuated using magnetic fields. C1 [Sabo, Ronald] US Forest Serv, USDA, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. [Yermakov, Aleksey] Univ Wisconsin, Dept Mech Engn, Milwaukee, WI 53211 USA. [Law, Chiu Tai] Univ Wisconsin, Dept Elect Engn & Comp Sci, Milwaukee, WI 53211 USA. [Elhajjar, Rani] Univ Wisconsin, Dept Civil & Environm Engn, Milwaukee, WI 53211 USA. RP Sabo, R (reprint author), US Forest Serv, USDA, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM rsabo@fs.fed.us NR 134 TC 0 Z9 0 U1 2 U2 2 PU SCRIVENER PUBLISHING LLC PI BEVERLY PA 100 CUMMINGS CENTER, SUITE 541J, BEVERLY, MA 01915-6106 USA SN 2164-6325 EI 2164-6341 J9 J RENEW MATER JI J. Renew. Mater. PD OCT PY 2016 VL 4 IS 5 BP 297 EP 312 DI 10.7569/JRM.2016.634114 PG 16 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Materials Science, Composites; Polymer Science SC Science & Technology - Other Topics; Materials Science; Polymer Science GA EL6RX UT WOS:000394750900001 ER PT J AU Lara, PN Plets, M Tangen, C Gertz, E Vogelzang, NJ Quinn, DI Thompson, I van Loan, M AF Lara, P. N. Plets, M. Tangen, C. Gertz, E. Vogelzang, N. J. Quinn, D. I. Thompson, I. van Loan, M. TI Bone biomarkers and overall survival (OS) in men with castrate resistant prostate cancer (CRPC) and skeletal metastases: Updated results from SWOG 0421, a phase III trial of docetaxel +/- atrasentan SO ANNALS OF ONCOLOGY LA English DT Meeting Abstract CT 41st Congress of the European-Society-for-Medical-Oncology (ESMO) CY OCT 07-11, 2016 CL Copenhagen, DENMARK SP European Soc Med Oncol C1 [Lara, P. N.] Univ Calif Davis, Ctr Canc, Internal Med Hematol Oncol, Sacramento, CA 95817 USA. [Plets, M.; Tangen, C.] Fred Hutchinson Canc Res Ctr, Stat, 1124 Columbia St, Seattle, WA 98104 USA. [Gertz, E.; van Loan, M.] Univ Calif Davis, USDA, Davis, CA 95616 USA. [Vogelzang, N. J.] Comprehens Canc Crts NV, US Oncol Res, Med Oncol, Las Vegas, NV USA. [Quinn, D. I.] Univ Southern Calif, Norris Comprehens Canc Ctr, Med, Los Angeles, CA USA. [Thompson, I.] Univ Texas Hlth Sci Ctr San Antonio, Urol, San Antonio, TX 78229 USA. FU National Cancer Institute (USA) FX National Cancer Institute (USA) NR 0 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0923-7534 EI 1569-8041 J9 ANN ONCOL JI Ann. Oncol. PD OCT 1 PY 2016 VL 27 SU 6 MA 754P DI 10.1093/annonc/mdw372.38 PG 1 WC Oncology SC Oncology GA EK4QS UT WOS:000393912500751 ER PT J AU Preece, JE Moersfelder, J AF Preece, John E. Moersfelder, Jeff TI Pomegranate: The Grainy Apple SO JOURNAL OF THE AMERICAN POMOLOGICAL SOCIETY LA English DT Article DE Punica granatum; fruit quality; germplasm C1 [Preece, John E.; Moersfelder, Jeff] Univ Calif Davis, USDA ARS, Natl Clonal Germplasm Repository, One Shields Ave, Davis, CA 95616 USA. RP Preece, JE (reprint author), Univ Calif Davis, USDA ARS, Natl Clonal Germplasm Repository, One Shields Ave, Davis, CA 95616 USA. EM John.Preece@ars.usda.gov NR 23 TC 0 Z9 0 U1 1 U2 1 PU AMER POMOLOGICAL SOC PI UNIVERSITY PK PA 102 TYSON BUILDING, UNIVERSITY PK, PA 16802 USA SN 1527-3741 J9 J AM POMOL SOC JI J. Amer. Pomolog. Soc. PD OCT PY 2016 VL 70 IS 4 BP 187 EP 193 PG 7 WC Agronomy; Horticulture SC Agriculture GA EJ9ZE UT WOS:000393585300003 ER PT J AU Kaur, N Hasegawa, DK Ling, KS Wintermantel, WM AF Kaur, Navneet Hasegawa, Daniel K. Ling, Kai-Shu Wintermantel, William M. TI Application of Genomics for Understanding Plant Virus-Insect Vector Interactions and Insect Vector Control SO PHYTOPATHOLOGY LA English DT Article ID WHITEFLY BEMISIA-TABACI; INFECTIOUS-YELLOWS-VIRUS; DEEP SEQUENCING ANALYSIS; TOMATO-CHLOROSIS-VIRUS; LEAF-CURL-VIRUS; RNA INTERFERENCE; FRANKLINIELLA-OCCIDENTALIS; SPECIES COMPLEX; AEDES-AEGYPTI; COAT PROTEIN AB The relationships between plant viruses and their vectors have evolved over the millennia, and yet, studies on viruses began <150 years ago and investigations into the virus and vector interactions even more recently. The advent of next generation sequencing, including rapid genome and transcriptome analysis, methods for evaluation of small RNAs, and the related disciplines of proteomics and metabolomics offer a significant shift in the ability to elucidate molecular mechanisms involved in virus infection and transmission by insect vectors. Genomic technologies offer an unprecedented opportunity to examine the response of insect vectors to the presence of ingested viruses through gene expression changes and altered biochemical pathways. This review focuses on the interactions between viruses and their whitefly or thrips vectors and on potential applications of genomics-driven control of the insect vectors. Recent studies have evaluated gene expression in vectors during feeding on plants infected with begomoviruses, criniviruses, and tospoviruses, which exhibit very different types of virus-vector interactions. These studies demonstrate the advantages of genomics and the potential complementary studies that rapidly advance our understanding of the biology of virus transmission by insect vectors and offer additional opportunities to design novel genetic strategies to manage insect vectors and the viruses they transmit. C1 [Kaur, Navneet; Wintermantel, William M.] USDA ARS, Crop Improvement & Protect Res, Salinas, CA 93905 USA. [Hasegawa, Daniel K.; Ling, Kai-Shu] USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. [Hasegawa, Daniel K.] Cornell Univ, Boyce Thompson Inst, Ithaca, NY 14853 USA. RP Wintermantel, WM (reprint author), USDA ARS, Crop Improvement & Protect Res, Salinas, CA 93905 USA. EM bill.wintermantel@ars.usda.gov FU USDA-ARS Area-Wide project grant; U.S. Agency for International Development (USAID) Feed-the-Future program [58-0210-3-012] FX This work was supported in part by a USDA-ARS Area-Wide project grant to K.-S. Ling and W. M. Wintermantel, and a grant from the U.S. Agency for International Development (USAID) Feed-the-Future program (58-0210-3-012) coordinated by the USDA-ARS Office of International Research Programs. NR 108 TC 1 Z9 1 U1 4 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD OCT PY 2016 VL 106 IS 10 BP 1213 EP 1222 DI 10.1094/PHYTO-02-16-0111-FI PG 10 WC Plant Sciences SC Plant Sciences GA DW7GV UT WOS:000383820100016 ER PT J AU Clemons, C Pilla, S AF Clemons, Craig Pilla, Srikanth TI Introduction to the Special Issue on Nanocellulose Composites SO JOURNAL OF RENEWABLE MATERIALS LA English DT Editorial Material C1 [Clemons, Craig] US Forest Serv, USDA, Forest Prod Lab, Washington, DC 20250 USA. [Pilla, Srikanth] Clemson Univ, Clemson, SC 29631 USA. RP Clemons, C (reprint author), US Forest Serv, USDA, Forest Prod Lab, Washington, DC 20250 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SCRIVENER PUBLISHING LLC PI BEVERLY PA 100 CUMMINGS CENTER, SUITE 541J, BEVERLY, MA 01915-6106 USA SN 2164-6325 EI 2164-6341 J9 J RENEW MATER JI J. Renew. Mater. PD OCT PY 2016 VL 4 IS 5 BP 295 EP 296 DI 10.7569/JRM.2016.634133 PG 2 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Materials Science, Composites; Polymer Science SC Science & Technology - Other Topics; Materials Science; Polymer Science GA EJ3SH UT WOS:000393131400001 ER PT J AU Stark, NM AF Stark, Nicole M. TI Opportunities for Cellulose Nanomaterials in Packaging Films: A Review and Future Trends SO JOURNAL OF RENEWABLE MATERIALS LA English DT Review DE Cellulose nanocrystals (CNCs); cellulose nanofibrils (CNFs); cellulose nanomaterials; packaging; films; composites; mechanical properties; barrier properties; water vapor transmission rate; oxygen transmission rate; modification ID WATER-VAPOR BARRIER; MICROFIBRILLATED CELLULOSE; NANOCOMPOSITE FILMS; POLY(LACTIC ACID); NANOCRYSTALLINE CELLULOSE; POSIDONIA-OCEANICA; PLA NANOCOMPOSITES; OPTICAL-PROPERTIES; POLYVINYL-ALCOHOL; COMPOSITE FILMS AB Performance requirements for packaging films may include barrier properties, transparency, flexibility, and tensile strength. Conventional packaging materials, such as plastic films and laminates, are typically made from petroleum-based polymers. Currently, there is a drive to develop sustainable packaging materials. These alternative materials must be able to be manufactured economically and on a commercial scale, exhibit barrier properties and transparency, and provide adequate mechanical performance. As a biobased, renewable material, cellulose nanomaterials (CNs) are ideally suited to be used in sustainable packaging applications. CNs include cellulose nanocrystals (CNCs) and cellulose nanofibrils (CNFs) and each can provide benefit to packaging films. Manufactured CNF films can be used as packaging films or laminates and have been shown to have good strength properties and good barrier properties, particularly at low humidity. Both CNCs and CNFs can be added to other polymers to improve strength and barrier properties. The flexibility of CNs to be used in a variety of ways in packaging applications has resulted in considerable attention and research activity. This article summarizes the current applicability for CNs in packaging films and discusses the future trends and opportunities for these materials. C1 [Stark, Nicole M.] US Forest Serv, USDA, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. RP Stark, NM (reprint author), US Forest Serv, USDA, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. EM nstark@fs.fed.us NR 96 TC 0 Z9 0 U1 8 U2 8 PU SCRIVENER PUBLISHING LLC PI BEVERLY PA 100 CUMMINGS CENTER, SUITE 541J, BEVERLY, MA 01915-6106 USA SN 2164-6325 EI 2164-6341 J9 J RENEW MATER JI J. Renew. Mater. PD OCT PY 2016 VL 4 IS 5 BP 313 EP 326 DI 10.7569/JRM.2016.634115 PG 14 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Materials Science, Composites; Polymer Science SC Science & Technology - Other Topics; Materials Science; Polymer Science GA EJ3SH UT WOS:000393131400003 ER PT J AU Clemons, C AF Clemons, Craig TI Nanocellulose in Spun Continuous Fibers: A Review and Future Outlook SO JOURNAL OF RENEWABLE MATERIALS LA English DT Review DE Nanocellulose; cellulose nanofibrils; cellulose nanocrystals; fiber; spinning ID REGENERATED CELLULOSE FIBERS; POLYVINYL-ALCOHOL; ELECTROSPUN NANOFIBERS; REINFORCED COMPOSITES; POLY(ETHYLENE OXIDE); POLY(VINYL ALCOHOL); NANOCRYSTALS; NANOCOMPOSITES; WHISKERS; NANOWHISKERS AB Continuous fibers are commonly manufactured for a wide variety of uses such as filters, textiles, and composites. For example, most fibrous reinforcements (e.g., carbon fiber, glass fiber) for advanced composites are continuous fibers or yarns, fabrics, and preforms made from them. This allows broad flexibility in design and manufacturing approaches by controlling fiber orientation and architecture. However, there has been growing interest in preparing continuous fibers from biobased materials such as plants. Of particular recent interest are nanocelluloses, which are projected to be less expensive than many other nanomaterials and have the potential to be produced in large volumes. They also have an impressive strength-to-weight ratio and have so far shown few environmental, health, and safety concerns in their unmodified state. However, efficient and effective use of nanocellulose in continuous fibers is challenging and a variety of approaches have been explored in which nanocellulose dispersions are either spun directly or in combination with polymers. Methods such as wet spinning, dry spinning, melt spinning, and electrospinning have been investigated. To better understand the body of knowledge of this new and growing area, various approaches are reviewed and a perspective on what the future holds is provided. C1 [Clemons, Craig] US Forest Serv, USDA, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. RP Clemons, C (reprint author), US Forest Serv, USDA, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. EM cclemons@fs.fed.us NR 71 TC 1 Z9 1 U1 12 U2 12 PU SCRIVENER PUBLISHING LLC PI BEVERLY PA 100 CUMMINGS CENTER, SUITE 541J, BEVERLY, MA 01915-6106 USA SN 2164-6325 EI 2164-6341 J9 J RENEW MATER JI J. Renew. Mater. PD OCT PY 2016 VL 4 IS 5 BP 327 EP 339 DI 10.7569/JRM.2016.634112 PG 13 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Materials Science, Composites; Polymer Science SC Science & Technology - Other Topics; Materials Science; Polymer Science GA EJ3SH UT WOS:000393131400004 ER PT J AU Eguiguren-Velepucha, PA Chamba, JAM Mendoza, NAA Ojeda-Luna, TL Samaniego-Rojas, NS Furniss, MJ Howe, C Mendoza, ZHA AF Alexander Eguiguren-Velepucha, Paul Maita Chamba, Juan Armando Aguirre Mendoza, Nikolay Arturo Lizbeth Ojeda-Luna, Tatiana Soledad Samaniego-Rojas, Natalia Furniss, Michael J. Howe, Carol Aguirre Mendoza, Zhofre Huberto TI Tropical ecosystems vulnerability to climate change in southern Ecuador SO TROPICAL CONSERVATION SCIENCE LA English DT Article DE biodiversity; drivers of change; exposure; sensitivity; adaptive capacity ID CHANGE IMPACTS; BIODIVERSITY; CONSERVATION; DISTRIBUTIONS; COMMUNITIES; PREDICTIONS; CHALLENGES; MODELS; FUTURE; GROWTH AB Tropical ecosystems are among the most vulnerable to climate change. Understanding climate impacts on these ecosystems is a primary challenge for policy makers, ecologists, and conservationists today. We analyzed the vulnerability of ecosystems in a very heterogeneous tropical region in southern Ecuador, selected because of its exceptional biodiversity and its ecosystem services provided to people of southern Ecuador and northern Peru. The vulnerability assessment focused on three components: exposure, sensitivity, and adaptive capacity. For the first two components, we identified stressors or drivers of change that negatively influence ecosystems. For the third component, we identified existing and potential buffers that reduce impacts. This process was developed in workshops and by expert elicitation. Representative Concentration Pathway (RCP) scenarios were used, considering RCP 2.6 and RCP 8.5 for a time horizon to 2050. Under the RCP 2.6 scenario, the components of overall vulnerability in the southern region of Ecuador showed very low to moderate vulnerability for most areas, particularly in semi-deciduous forest ecosystems, Amazon semi-deciduous forest, Amazon rainforest, and mangrove forests. These areas had high vulnerability under the RCP 8.5 scenario. A variety of conservation strategies (e.g., protected areas) were shown to increase the adaptive capacity of ecosystems and reduce their vulnerability. We therefore recommend improving these conservation initiatives in ecosystems like dry forests, where the greatest vulnerability is evident. C1 [Alexander Eguiguren-Velepucha, Paul; Maita Chamba, Juan Armando; Aguirre Mendoza, Nikolay Arturo; Lizbeth Ojeda-Luna, Tatiana; Soledad Samaniego-Rojas, Natalia] Univ Nacl Loja, Biodivers & Ecosyst Serv Res Program, EC-110103 Loja, Ecuador. [Furniss, Michael J.] Humboldt State Univ, Dept Environm Engn, Arcata, CA 95521 USA. [Furniss, Michael J.; Howe, Carol] US Forest Serv, USDA, Washington, DC 20250 USA. [Aguirre Mendoza, Zhofre Huberto] Univ Nacl Loja, Herbarium Loja Reinaldo Espinosa, Loja, Ecuador. RP Eguiguren-Velepucha, PA (reprint author), Univ Nacl Loja, Biodivers & Ecosyst Serv Res Program, EC-110103 Loja, Ecuador. EM pauleguiguren@gmail.com FU United States Agency for International Development FX We thank Universidad Nacional de Loja, the United States Forest Service for the scientific advisor; and the United States Agency for International Development for the financial support. NR 80 TC 0 Z9 0 U1 5 U2 5 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1940-0829 J9 TROP CONSERV SCI JI Trop. Conserv. Sci. PD OCT-DEC PY 2016 VL 9 IS 4 DI 10.1177/1940082916668007 PG 17 WC Biodiversity Conservation SC Biodiversity & Conservation GA EJ4SS UT WOS:000393207800004 ER PT J AU Burley, JD Bytnerowicz, A Buhler, M Zielinska, B Schweizer, D Cisneros, R Schilling, S Varela, JC McDaniel, M Horn, M Dulen, D AF Burley, Joel D. Bytnerowicz, Andrzej Buhler, Monica Zielinska, Barbara Schweizer, Donald Cisneros, Ricardo Schilling, Susan Varela, Jennifer Chapman McDaniel, Mark Horn, Michelle Dulen, Deanna TI Air Quality at Devils Postpile National Monument, Sierra Nevada Mountains, California, USA SO AEROSOL AND AIR QUALITY RESEARCH LA English DT Article DE Ozone; Passive samplers; PM2.5; VOC; Nitrogen oxides ID SURFACE OZONE; PARTICULATE MATTER; DRY DEPOSITION; WILDLAND FIRE; VARIABILITY; ENVIRONMENT; POLLUTION; FORESTS; DESERT; HEALTH AB Ambient concentrations of O-3, PM2.5, NH3, NO, NO2, HNO3, SO2 and VOCs were measured at Devils Postpile National Monument (DEPO) during the summer seasons of 2013 and 2014. The measurements were impacted by the Aspen and Rim Fires in 2013, and the French and King Fires in 2014. While O-3 concentrations were not discernibly perturbed by the fire events, the 70 ppb threshold (8-hour average) corresponding to both the current California Ambient Air Quality Standard (CAAQS) and the new National Ambient Air Quality Standard (NAAQS) was exceeded on five days during 2013, and on 16 days during 2014. The older NAAQS of 75 ppb (8-hour average) was exceeded once in 2013, and six times in 2014. Exceedances of the CAAQS or NAAQS occurred when background sources of O-3 were augmented by regional-scale transport, at higher altitudes, of polluted air masses that had passed through the San Joaquin Valley before arriving at the DEPO site. The 2013 Aspen Fire elevated PM2.5 to a maximum hourly concentration of 214 mu g m(-3) and a maximum 24 h mean of 92.7 mu g m(-3), and resulted in 13 exceedances of the 35 mu g m(-3) (24 h average) NAAQS for PM2.5. The 2013 Rim Fire increased PM2.5 to a maximum hourly concentration of 132 mu g m(-3) and a maximum 24 h mean of 69.6 mu g m(-3), and resulted in two exceedances of the 24 h NAAQS. Concentrations of NH3 increased during all fires, as did those of NO2 during the Aspen and Rim Fires. Concentrations of benzene increased substantially during the French Fire. C1 [Burley, Joel D.; Varela, Jennifer Chapman] St Marys Coll Calif, Dept Chem, Moraga, CA 94575 USA. [Bytnerowicz, Andrzej; Schilling, Susan] USDA Forest Serv, PSW Res Stn, Riverside, CA 92507 USA. [Buhler, Monica; Dulen, Deanna] Devils Postpile Natl Monument, Mammoth Lakes, CA 93546 USA. [Zielinska, Barbara; McDaniel, Mark] Desert Res Inst, Reno, NV 89512 USA. [Schweizer, Donald; Cisneros, Ricardo] Univ Calif, Merced, CA 95340 USA. [Horn, Michelle] Tech Univ Munich, D-85354 Freising Weihenstephan, Germany. RP Burley, JD (reprint author), St Marys Coll Calif, Dept Chem, Moraga, CA 94575 USA. EM jburley@stmarys-ca.edu FU USDA Forest Service Pacific Southwest Research Station; National Park Service FX This study was funded by the USDA Forest Service Pacific Southwest Research Station and the National Park Service. We thank Diane Alexander, Reginald King, Mark Williams, Diana Lopez and Pegdwende Kologo for preparing and analyzing passive samplers, and Catherine Fong and Andrew Osborne for passive sampler deployment and changes. The authors gratefully acknowledge the NOAA Air Resources Laboratory (ARL) for the provision of the HYSPLIT transport and dispersion model and/or READY website (http://www.ready.noaa.gov) used in this publication. NR 52 TC 0 Z9 0 U1 0 U2 0 PU TAIWAN ASSOC AEROSOL RES-TAAR PI TAICHUNG COUNTY PA CHAOYANG UNIV TECH, DEPT ENV ENG & MGMT, PROD CTR AAQR, NO 168, JIFONG E RD, WUFONG TOWNSHIP, TAICHUNG COUNTY, 41349, TAIWAN SN 1680-8584 EI 2071-1409 J9 AEROSOL AIR QUAL RES JI Aerosol Air Qual. Res. PD OCT PY 2016 VL 16 IS 10 BP 2315 EP 2332 DI 10.4209/aaqr.2016.02.0069 PG 18 WC Environmental Sciences SC Environmental Sciences & Ecology GA EI2GK UT WOS:000392304700001 ER PT J AU Kesoju, SR Boydston, RA Greene, SL AF Kesoju, Sandya R. Boydston, Rick A. Greene, Stephanie L. TI Effect of Synthetic Auxin Herbicides on Seed Development and Viability in Genetically Engineered Glyphosate-Resistant Alfalfa SO WEED TECHNOLOGY LA English DT Article DE Adventitious presence; feral; gene flow; genetically engineered; glyphosate resistant; synthetic auxin herbicides ID MEDICAGO-SATIVA L.; NO-TILLAGE CORN; GENE FLOW; MANAGEMENT; FECUNDITY; ECOLOGY; PROGENY; TRAITS; GROWTH; 2,4-D AB Feral populations of cultivated crops have the potential to function as bridges and reservoirs that contribute to the unwanted movement of novel genetically engineered (GE) traits. Recognizing that feral alfalfa has the potential to lower genetic purity in alfalfa seed production fields when it is growing in the vicinity of foraging pollinators in alfalfa seed fields, industry has established production standards to control feral plants. However, with the commercialization of GE glyphosate-resistant (GR) alfalfa and the need to support the coexistence of both GE and conventional production, effective methods to control transgenic feral alfalfa need to be developed. Therefore, a study was conducted in 2012, 2013, and 2014 to determine the effect of several synthetic auxin herbicides on seed development in GR alfalfa. GR alfalfa, var. Genuity (R44BD16), was treated with dicamba, 2,4-D, triclopyr, and aminopyralid when alfalfa plants contained green seed pods. Two weeks after herbicide application, plants were harvested, air dried, and seed yield, seed germination, and seedling emergence from the soil were determined. In 2013, dicamba, triclopyr, and 2,4-D decreased alfalfa seed yield per plant compared wih nontreated plants, whereas in 2014, all four herbicides decreased alfalfa seed yield per plant 24 to 49% (by weight) compared with nontreated plants. The same trend was evident in 2012, but seed yield was variable and was not significantly different among treatments. Seed germination averaged 43, 50, and 72% in 2012, 2013, and 2014, respectively, and was not affected by the four herbicides applied at early pod-fill stage. However, seeds harvested from plants treated with dicamba, 2,4-D, and triclopyr often produced deformed and abnormal seedlings, and when planted in soil, frequently failed to emerge. The combined effects of dicamba, 2,4-D, and triclopyr in reducing seed yield, seedling emergence, and seedling growth could contribute to managing feral alfalfa populations. C1 [Kesoju, Sandya R.] Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. [Boydston, Rick A.] ARS, Legume Genet & Physiol Res Unit, USDA, Prosser, WA 99350 USA. [Greene, Stephanie L.] ARS, Plant & Anim Genet Resources Preservat Unit, USDA, Ft Collins, CO 80521 USA. RP Kesoju, SR (reprint author), Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. EM sandya.kesoju@wsu.edu FU U.S. Department of Agriculture-National Institute of Food and Agriculture [2011-33522-30733] FX The authors are grateful to Biotechnology Risk Assessment Grant Program # 2011-33522-30733 from the U.S. Department of Agriculture-National Institute of Food and Agriculture for funding of this research. We thank Treva Anderson and Encarnacion Rivera for their technical assistance and Bernardo Chaves-Cordoba for statistical analysis of the data. NR 42 TC 0 Z9 0 U1 1 U2 1 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0890-037X EI 1550-2740 J9 WEED TECHNOL JI Weed Technol. PD OCT-DEC PY 2016 VL 30 IS 4 BP 860 EP 868 DI 10.1614/WT-D-16-00045.1 PG 9 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA EI4EZ UT WOS:000392447200004 ER PT J AU Solaiman, DKY Ashby, RD Crocker, NV AF Solaiman, Daniel K. Y. Ashby, Richard D. Crocker, Nicole V. TI Genetic construction of recombinant Pseudomonas chlororaphis for improved glycerol utilization SO BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY LA English DT Article DE Glycerol metabolism; Biodegradable polymer; Biosurfactant ID AERUGINOSA; METABOLISM AB This study is to use genetic engineering to improve the glycerol metabolic capability of Pseudomonas chlororaphis which is capable of producing commercially valuable biodegradable poly(hydroxyalkanoate) (PHA) biopolymers and biosurfactant rhamnolipids (RLs). In the study, the glycerol uptake facilitator or aquaglyceroporin gene (glpF) and the glycerol kinase (g1pK) gene were PCR-cloned from E. coli, inserted into a shuttle vector pBS29P2-gfp, and expressed in P. chlororaphis by a Pseudomonas promoter P2. The P. chlororaphis recombinants were then tested for cell growth and glycerol metabolism in chemically defined medium containing 0.5% and 1.0% (v/v) glycerol. The simultaneous expression of glpF and glpK resulted in a shorter lag time for cell growth and a more immediate glycerol consumption by P. chlororaphis. In conclusion, the recombinant P. chlororaphis that grows more efficiently in glycerol is expected to improve the technoeconomics of PHA and RL production using the surplus bioglycerol byproduct stream from biodiesel production. (C) 2016 Published by Elsevier Ltd. C1 [Solaiman, Daniel K. Y.; Ashby, Richard D.; Crocker, Nicole V.] ARS, Eastem Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. RP Solaiman, DKY (reprint author), ARS, Eastem Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM dan.solaiman@ars.usda.gov NR 15 TC 0 Z9 0 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1878-8181 J9 BIOCATAL AGRIC BIOTE JI Biocatal. Agric. Biotechnol. PD OCT PY 2016 VL 8 BP 45 EP 49 DI 10.1016/j.bcab.2016.08.001 PG 5 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA EG2NP UT WOS:000390880700008 ER PT J AU Qu, WC Robert, CAM Erb, M Hibbard, BE Paven, M Gleede, T Riehl, B Kersting, L Cankaya, AS Kunert, AT Xu, Y Schueller, MJ Shea, C Alexoff, D Lee, SJ Fowler, JS Ferrieri, RA AF Qu, Wenchao Robert, Christelle A. M. Erb, Matthias Hibbard, Bruce E. Paven, Maxim Gleede, Tassilo Riehl, Barbara Kersting, Lena Cankaya, Aylin S. Kunert, Anna T. Xu, Youwen Schueller, Michael J. Shea, Colleen Alexoff, David Lee, So Jeong Fowler, Joanna S. Ferrieri, Richard A. TI Dynamic Precision Phenotyping Reveals Mechanism of Crop Tolerance to Root Herbivory SO PLANT PHYSIOLOGY LA English DT Article ID WESTERN CORN-ROOTWORM; AUXIN BIOSYNTHESIS; RAPID CHANGES; ARABIDOPSIS-THALIANA; CARBON TRANSPORT; MICROPET R4; ACID; PLANTS; TRYPTOPHAN; DAMAGE AB The western corn rootworm (WCR; Diabrotica virgifera virgifera LeConte) is a major pest of maize (Zea mays) that is well adapted to most crop management strategies. Breeding for tolerance is a promising alternative to combat WCR but is currently constrained by a lack of physiological understanding and phenotyping tools. We developed dynamic precision phenotyping approaches using C-11 with positron emission tomography, root autoradiography, and radiometabolite flux analysis to understand maize tolerance to WCR. Our results reveal that WCR attack induces specific patterns of lateral root growth that are associated with a shift in auxin biosynthesis from indole-3-pyruvic acid to indole-3-acetonitrile. WCR attack also increases transport of newly synthesized amino acids to the roots, including the accumulation of Gln. Finally, the regrowth zones of WCR-attacked roots show an increase in Gln turnover, which strongly correlates with the induction of indole-3-acetonitrile-dependent auxin biosynthesis. In summary, our findings identify local changes in the auxin biosynthesis flux network as a promising marker for induced WCR tolerance. C1 [Qu, Wenchao; Xu, Youwen; Schueller, Michael J.; Shea, Colleen; Alexoff, David; Lee, So Jeong; Fowler, Joanna S.; Ferrieri, Richard A.] Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA. [Robert, Christelle A. M.; Erb, Matthias] Univ Bern, Inst Plant Sci, Biot Interact, CH-3013 Bern, Switzerland. [Hibbard, Bruce E.] Univ Missouri, USDA ARS, Plant Genet Res Unit, Columbia, MO 65211 USA. [Paven, Maxim; Gleede, Tassilo; Riehl, Barbara; Kersting, Lena; Cankaya, Aylin S.; Kunert, Anna T.] Johannes Gutenberg Univ Mainz, Inst Kernchem, D-55128 Mainz, Germany. [Qu, Wenchao] Cornell Univ, Weill Cornell Med, New York, NY 10065 USA. [Paven, Maxim] Max Planck Inst Polymer Res, Ackermannweg 10, D-55128 Mainz, Germany. [Kunert, Anna T.] Max Planck Inst Chem, Hahn Meitner Weg 1, D-55128 Mainz, Germany. [Ferrieri, Richard A.] Univ Missouri, Dept Chem, MURR, 1513 Res Pk Dr, Columbia, MO 65211 USA. [Lee, So Jeong] Univ Michigan, Dept Radiol, Med Ctr Dr, 1500 E Med Ctr Dr, Ann Arbor, MI 48109 USA. RP Ferrieri, RA (reprint author), Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA.; Ferrieri, RA (reprint author), Univ Missouri, Dept Chem, MURR, 1513 Res Pk Dr, Columbia, MO 65211 USA. EM ferrierir@missouri.edu FU United States Department of Energy, Office of Biological and Environmental Research [DE-AC02-98CH10886]; German Academic Exchange Service (Deutscher Akademischer Austauschdienst, DAAD) Bonn; Marie Curie Intra-European Fellowship program; Swiss National Foundation Fellowship program; United States Government FX This article has been authored by Brookhaven Science Associates, LLC, under contract number DE-AC02-98CH10886 with the United States Department of Energy, Office of Biological and Environmental Research. Additional support was provided by the German Academic Exchange Service (Deutscher Akademischer Austauschdienst, DAAD) Bonn, the Marie Curie Intra-European Fellowship program, and the Swiss National Foundation Fellowship program. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a nonexclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. NR 54 TC 1 Z9 1 U1 8 U2 8 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD OCT PY 2016 VL 172 IS 2 BP 776 EP 788 DI 10.1104/pp.16.00735 PG 13 WC Plant Sciences SC Plant Sciences GA EG6II UT WOS:000391147700013 PM 27406166 ER PT J AU Bo, KL Wang, H Pan, YP Behera, TK Pandey, S Wen, CL Wang, YH Simon, PW Li, YH Chen, JF Weng, YQ AF Bo, Kailiang Wang, Hui Pan, Yupeng Behera, Tusar K. Pandey, Sudhakar Wen, Changlong Wang, Yuhui Simon, Philipp W. Li, Yuhong Chen, Jinfeng Weng, Yiqun TI SHORT HYPOCOTYL1 Encodes a SMARCA3-Like Chromatin Remodeling Factor Regulating Elongation SO PLANT PHYSIOLOGY LA English DT Article ID TRANSCRIPTION FACTOR HY5; LIGHT SIGNAL-TRANSDUCTION; B PHOTORECEPTOR UVR8; SOLAR ULTRAVIOLET-RADIATION; SYNTHASE GENE-EXPRESSION; CUCUMBER CUCUMIS-SATIVUS; QUANTITATIVE TRAIT LOCI; ARABIDOPSIS-THALIANA; BLUE-LIGHT; HYPOCOTYL GROWTH AB In Arabidopsis (Arabidopsis thaliana), the UVR8-mediated signaling pathway is employed to attain UVB protection and acclimation to deal with low-dosage UVB (LDUVB)-induced stresses. Here, we identified SHORT HYPOCOTYL1 (SH1) in cucumber (Cucumis sativus), which regulates LDUVB-dependent hypocotyl elongation by modulating the UVR8 signaling pathway. We showed that hypocotyl elongation in cucumbers carrying the recessive sh1 allele was LDUVB insensitive and that Sh1 encoded a human SMARCA3-like chromatin remodeling factor. The allele frequency and distribution pattern at this locus among natural populations supported the wild cucumber origin of sh1 for local adaptation, which was under selection during domestication. The cultivated cucumber carries predominantly the Sh1 allele; the sh1 allele is nearly fixed in the semiwild Xishuangbanna cucumber, and the wild cucumber population is largely at Hardy-Weinberg equilibrium for the two alleles. The SH1 protein sequence was highly conserved among eukaryotic organisms, but its regulation of hypocotyl elongation in cucumber seems to be a novel function. While Sh1 expression was inhibited by LDUVB, its transcript abundance was highly correlated with hypocotyl elongation rate and the expression level of cell-elongation-related genes. Expression profiling of key regulators in the UVR8 signaling pathway revealed significant differential expression of CsHY5 between two near isogenic lines of Sh1. Sh1 and CsHY5 acted antagonistically at transcriptional level. A working model was proposed in which Sh1 regulates LDUVB-dependent hypocotyl elongation in cucumber through changing the chromatin states and thus the accessibility of CsHY5 in the UVR8 signaling pathway to promoters of LDUVB-responsive genes for hypocotyl elongation. C1 [Bo, Kailiang; Pan, Yupeng; Wang, Yuhui; Simon, Philipp W.; Weng, Yiqun] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Bo, Kailiang; Chen, Jinfeng] Nanjing Agr Univ, Hort Coll, Nanjing 210095, Jiangsu, Peoples R China. [Wang, Hui; Pan, Yupeng; Li, Yuhong] Northwest A&F Univ, Hort Coll, Yangling 712100, Peoples R China. [Behera, Tusar K.] Indian Agr Res Inst, Div Vegetable Sci, New Delhi 10012, India. [Pandey, Sudhakar] Indian Inst Vegetable Res, Indian Council Agr Res, Div Crop Improvement, Varanasi 221305, Uttar Pradesh, India. [Wen, Changlong] Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China. [Wen, Changlong] Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Beijing 100097, Peoples R China. [Simon, Philipp W.; Weng, Yiqun] ARS, Vegetable Crops Res Unit, USDA, Madison, WI 53706 USA. RP Weng, YQ (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA.; Chen, JF (reprint author), Nanjing Agr Univ, Hort Coll, Nanjing 210095, Jiangsu, Peoples R China.; Weng, YQ (reprint author), ARS, Vegetable Crops Res Unit, USDA, Madison, WI 53706 USA. EM jfchen@njau.edu.cn; yiqun.weng@wisc.edu FU United States Department of Agriculture Specialty Crop Research Initiative grant (SCRI) [2011-51181-30661]; National Natural Science Foundation of China [31171955, 31471891, 31430075] FX This work was supported by a United States Department of Agriculture Specialty Crop Research Initiative grant (SCRI, project no. 2011-51181-30661) to Y.We. The work in Y.L.'s lab was supported by the National Natural Science Foundation of China (31171955 and 31471891). J.C.'s work was supported by the National Natural Science Foundation of China (31430075). NR 108 TC 0 Z9 0 U1 3 U2 3 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD OCT PY 2016 VL 172 IS 2 BP 1273 EP 1292 DI 10.1104/pp.16.00501 PG 20 WC Plant Sciences SC Plant Sciences GA EG6II UT WOS:000391147700049 PM 27559036 ER PT J AU Neto, TDN Gardner, D Hallwass, F Leite, AJM de Almeida, CG Silva, LN Roque, AD de Bitencourt, FG Barbosa, EG Tasca, T Macedo, AJ de Almeida, MV Giordani, RB AF Negreiros Neto, Themistocles da Silva Gardner, Dale Hallwass, Fernando Matias Leite, Ana Jessica de Almeida, Camila Guimaraes Silva, Laura Nunes Roque, Alan de Araujo de Bitencourt, Fernanda Gobbi Barbosa, Euzebio Guimaraes Tasca, Tiana Macedo, Alexandre Jose de Almeida, Mauro Vieira Giordani, Raquel Brandt TI Activity of pyrrolizidine alkaloids against biofilm formation and Trichomonas vaginalis SO BIOMEDICINE & PHARMACOTHERAPY LA English DT Article DE Usaramine; Monocrotaline; Pyrrolizidine alkaloids; Biological activity ID BRAZILIAN SEMIARID REGION; BACTERIAL BIOFILMS; DOUBLE-BLIND; INFECTIONS; VIRULENCE; OINTMENT; SYMPHYTI; EFFICACY; CAATINGA AB Crotalaria genus belongs to the subfamily Papilionoideae comprising about 600 species spread throughout tropical, neotropical and subtropical regions. In this study, seeds of Crolatalaria pallida were used to the isolation of usaramine, a pyrrolizidine alkaloid. Thus, Pseudomonas aeruginosa and Staphylococcus epidermidis were utilized as strains to test some activities of this alkaloid, such as antibiofilm and antibacterial. Meanwhile, monocrotaline obtained from Crotalaria retusa seeds, was used as the starting material for synthesis of necine base derivatives with anti-Trichomonas vaginalis potential. Alkaloids were characterized by 1D and 2D NMR techniques and GC-MS analysis. Usaramine demonstrated a highlighted antibiofilm activity against S. epidermidis by reducing more than 50% of biofilm formation without killing the bacteria, thus it could be assumed as a prototype for the development of new antibiofilm molecules for pharmaceutical and industrial purposes. Monocrotaline activity against T. vaginalis was evaluated and results indicated inhibition of 80% on parasite growth at 1 mg/mL, in addition, neither cytotoxicity against vaginal epithelial cells nor hemolytic activity were observed. On the other hand, retronecine showed no anti-T. vaginalis activity while azido-retronecine was more active than monocrotaline killing 85% of the parasites at 1 mg/mL. In conclusion, pyrrolizidine alkaloids are suggested as promising prototypes for new drugs especially for topical use. (C) 2016 Elsevier Masson SAS. All rights reserved. C1 [Negreiros Neto, Themistocles da Silva; Matias Leite, Ana Jessica; Barbosa, Euzebio Guimaraes; Giordani, Raquel Brandt] Univ Fed Rio Grande do Norte, Dept Farm, Gustavo Cordeiro de Faria SN, BR-59010180 Natal, RN, Brazil. [Gardner, Dale] ARS, USDA, Poisonous Plant Res Lab, 1150 East 1400 North, Logan, UT 84341 USA. [Hallwass, Fernando] Univ Fed Pernambuco, Dept Quim Fundamental, Ave Prof Moraes Rego 1235, BR-50670901 Recife, PE, Brazil. [de Almeida, Camila Guimaraes; de Almeida, Mauro Vieira] Univ Fed Juiz de Fora, Inst Ciencias Exatas, Dept Quim, Campus Martelos, BR-36036330 Juiz De Fora, MG, Brazil. [Silva, Laura Nunes; Macedo, Alexandre Jose] Univ Fed Rio Grande do Sul, Ctr Biotecnol, Ave Ipiranga 2752, BR-90610000 Porto Alegre, RS, Brazil. [Silva, Laura Nunes; Macedo, Alexandre Jose] Univ Fed Rio Grande do Sul, Fac Farm, Ave Ipiranga 2752, BR-90610000 Porto Alegre, RS, Brazil. [Roque, Alan de Araujo] Univ Fed Rio Grande do Norte, Herbario UFRN, Campus Univ Lagoa Nova, BR-59078970 Natal, RN, Brazil. [de Bitencourt, Fernanda Gobbi; Tasca, Tiana] Univ Fed Rio Grande do Sul, Fac Farm, Lab Pesquisa Parasitol, Ave Ipiranga 2752, BR-90610000 Porto Alegre, RS, Brazil. RP Giordani, RB (reprint author), Univ Fed Rio Grande do Norte, Dept Farm, Gustavo Cordeiro de Faria SN, BR-59010180 Natal, RN, Brazil. EM theminegreiros@yahoo.com.br; dale.gardner@ars.usda.gov; hallwass@ufpe.br; ana@analeite.org; camilagdealmeida@gmail.com; lauransilva@gmail.com; alan.ufrn@gmail.com; fe_gobbi@hotmail.com; euzebiobg@gmail.com; tiana.tasca@ufrgs.br; alexandre.macedo@ufrgs.br; mauro.almeida@ufjf.edu.br; raquebg@hotmail.com FU Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil; CNPq (Brazil) [471209/2011-2]; FAPEMIG; NANOBIOTEC-Brazil program from CAPES; FAPERGS; CNPq [307447/2014-6] FX T.S.N.N. acknowledgethe Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil, for research fellowship. This work was supported by a CNPq (Brazil) grant [471209/2011-2] awarded to R.B.G., FAPEMIG and by the NANOBIOTEC-Brazil program from CAPES and FAPERGS awarded to A.J.M. T.T. thanks CNPq for researcher fellowship (grant 307447/2014-6). NR 35 TC 0 Z9 0 U1 3 U2 3 PU ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER PI PARIS PA 23 RUE LINOIS, 75724 PARIS, FRANCE SN 0753-3322 EI 1950-6007 J9 BIOMED PHARMACOTHER JI Biomed. Pharmacother. PD OCT PY 2016 VL 83 BP 323 EP 329 DI 10.1016/j.biopha.2016.06.033 PG 7 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA EF6IC UT WOS:000390433400040 ER PT J AU Njoroge, AW Affognon, H Mutungi, C Rohde, B Richter, U Hensel, O Mankin, RW AF Njoroge, A. W. Affognon, H. Mutungi, C. Rohde, B. Richter, U. Hensel, O. Mankin, R. W. TI Frequency and time pattern differences in acoustic signals produced by Prostephanus truncatus (Horn) (Coleoptera: Bostrichidae) and Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae) in stored maize SO JOURNAL OF STORED PRODUCTS RESEARCH LA English DT Article DE Detection; Sensor; Pest management; Postharvest ID RHYNCHOPHORUS-FERRUGINEUS; GRAIN; INFESTATION; ORYZAE; PESTS; WHEAT; DRYOPHTHORIDAE; DETECTABILITY; OVIPOSITION; POPULATIONS AB Frequency spectra and timing patterns of brief, 1-10 ms broadband sound impulses produced by movement and feeding activities of Prostephanus truncatus and Sitophilus zeamais last instars and adults in maize were investigated to find spectral and temporal pattern information useful for distinguishing among these species and stages. The impulse spectra were categorized into five different types of frequency patterns (profiles), designated Broadband, HighF, MidF1, MidF2 and LowF to indicate differences in their peak energies and broadness of frequency range. Groups (trains) of three or more closely spaced impulses, termed bursts, were observed to occur frequently in all recordings, as has been reported for sounds produced by other insects. Mean rates of bursts, mean counts of impulses per burst, and mean rates of impulses in bursts were calculated and compared among the two species and stages. The counts of broadband and MidF2 impulses per burst and the rates of broadband and MidF2 impulses in bursts were significantly different for adult than for 4th instar S. zeamais and either stage of P. truncatus. These findings can be useful in developing an acoustic sensor system for automated detection of hidden insects including P. truncatus and S. zeamais in bulk storage warehouses. The findings are discussed in relation to different movement and feeding behavior patterns that have been identified in these important pests. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Njoroge, A. W.; Mutungi, C.] Int Ctr Insect Physiol & Ecol, POB 30772, Nairobi 00100, Kenya. [Njoroge, A. W.; Richter, U.; Hensel, O.] Univ Kassel, Fac Agr Engn, Nordbahnhofstr 1a, D-37213 Witzenhausen, Germany. [Affognon, H.] Int Crops Res Inst Semi Arid Trop, BP 320, Bamako, Mali. [Rohde, B.] Univ Florida, Dept Elect & Comp Engn, Gainesville, FL 32611 USA. [Mankin, R. W.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Njoroge, AW (reprint author), POB 30772, Nairobi 00100, Kenya. EM anjoroge@icipe.org NR 43 TC 1 Z9 1 U1 2 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-474X EI 1879-1212 J9 J STORED PROD RES JI J. Stored Prod. Res. PD OCT PY 2016 VL 69 BP 31 EP 40 DI 10.1016/j.jspr.2016.06.005 PG 10 WC Entomology SC Entomology GA EF7MY UT WOS:000390514900006 ER PT J AU Yee, WL Goughnour, RB AF Yee, Wee L. Goughnour, Robert B. TI Peach is an occasional host for Rhagoletis pomonella (Walsh, 1867) (Diptera: Tephritidae) larvae in western Washington state, USA SO PAN-PACIFIC ENTOMOLOGIST LA English DT Article DE apple maggot; Prunus persica; Malus domestica ID CHERRY FRUIT-FLY; APPLE MAGGOT; PLANT USE AB Peach, Prunus persica (L.) Batsch (Rosaceae), has been reported to be a host of the apple maggot fly, Rhagoletis pomonella (Walsh, 1867) (Diptera: Tephritidae), an important quarantine pest of apple (Malus domestica Borkhausen) (Rosaceae) in the western U.S.A. However, all reports of peach as a host appear unreliable. Here, we tested the hypothesis that apple maggot does not utilize peaches in western Washington state. In 2013, unbaited sticky yellow rectangle traps were hung in a peach orchard similar to 50 m from neglected apple trees in Vancouver, Washington. During 2013-2015, peaches in the orchard were collected, peaches were hung in infested apple trees, and peaches were exposed to flies in the laboratory. In 2013, 24 flies were caught on 200 traps in the peach orchard, but no larvae emerged from 4,243 'Alberta' or 'Red-haven' peaches collected under similar to 200 trees in 2013 and 2014 or from peaches hung in apple trees in 2013. In 2015, however, 24 apple maggot larvae emerged from 804 `Starfire' or `PF 23' peaches, specifically from collections under two of 13 trees. In addition, in one of four laboratory tests, 11 larvae emerged from 'Redhaven' peaches exposed to apple maggot flies. Contrary to our prediction, results definitively show that peach can be an occasional developmental host for apple maggot larvae in the field and that apple maggot flies can be successfully reared using peach as a host in the laboratory. C1 [Yee, Wee L.] USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. [Goughnour, Robert B.] Washington State Univ, CAHNRS Extens, Vancouver, WA 98665 USA. RP Yee, WL (reprint author), USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM wee.yee@ars.usda.gov; Robert.Goughnour@clark.wa.gov FU USDA-Foreign Agricultural Service FX We thank Doug Stienbarger at Washington State University Vancouver for providing research facilities, Peter Chapman for taking the photographs of the puparia, Gene Miliczky for obtaining walnut husk fly puparia, Michael Klaus (Washington State Department of Agriculture) and Juan Rull (PROIMI Biotecnologia-CONICET, LIEMEN-Division Control Biologico de Plagas, Tucuman, Argentina) for reviewing earlier drafts of the manuscript, Brad Sinclair (Canadian National Collection of Insects and Ottawa Plant Laboratory - Entomology, Canadian Food Inspection Agency, Ottawa, Ontario, Canada) and an anonymous reviewer for valuable comments on the manuscript, and the USDA-Foreign Agricultural Service for partially funding this work. NR 27 TC 0 Z9 0 U1 1 U2 1 PU PACIFIC COAST ENTOMOL SOC PI SAN FRANCISCO PA C/O CALIFORNIA ACADEMY SCIENCES, 875 HOWARD STREET, SAN FRANCISCO, CA 94103-3009 USA SN 0031-0603 EI 2162-0237 J9 PAN-PAC ENTOMOL JI Pan-Pacific Entomol. PD OCT PY 2016 VL 92 IS 4 BP 189 EP 199 PG 11 WC Entomology SC Entomology GA EG3XD UT WOS:000390977100002 ER PT J AU Pratt, PD Herdocia, K Valentin, V Makinson, J Purcell, MF Mattison, E Rayamajhi, MB Moran, P Raghu, S AF Pratt, P. D. Herdocia, K. Valentin, V. Makinson, J. Purcell, M. F. Mattison, E. Rayamajhi, M. B. Moran, P. Raghu, S. TI Development rate, consumption, and host fidelity of Neostauropus alternus (Walker, 1855) (Lepidoptera: Notodontidae) SO PAN-PACIFIC ENTOMOLOGIST LA English DT Article DE Host use; weed biological control; assimilation; Rhodomyrtus tomentosa ID RHODOMYRTUS-TOMENTOSA MYRTACEAE; WHEAT CURL MITE; BIOLOGICAL-CONTROL; COMPLEX; WEED; SPECIATION; INSECTS; ACARI AB The Asian shrub Rhodomyrtus tomentosa (Aiton) Hasskarl (Myrtaceae) is an invasive weed in Florida and Hawaii, U.S.A. Surveys for natural enemies of this exotic shrub in Hong Kong, China resulted in the development of a laboratory colony and host range testing of Neostauropus alternus (Walker, 1855) as a potential biological control agent of R. tomentosa. Twelve critical test plant species were presented to larvae of N alternus. Complete development was limited to R. tomentosa, the ornamentally important Myrtus communis Linneaus (Myrtaceae), and the Florida natives Myrcianthes fragrans (Small) (K.A. Wilson) (Myrtaceae) and Morella cerifera (Linneaus) Small (Myricaceae). Total development time was more than ten days faster with R tomentosa versus M. communis and M. cerifera, with the latter species requiring an extra larval instar to reach the pupal stage. Consumption rates were similar among R. tomentosa and M. cerifera, but due to the longer development time, larvae consumed two-fold more leaf material on M. cerifera. Despite an apparent larval survival and development preference for R. tomentosa, it is clear that the physiological host range of N alternus includes M. cerifera and M. communis. The generalized feeding patterns exhibited in this research indicate that additional resources dedicated to the development of N alternus as a biological control agent of R. tomentosa in Florida are unwarranted. C1 [Pratt, P. D.; Moran, P.] USDA ARS, Exot & Invas Weeds Res Unit, 800 Buchanan St, Albany, CA 94710 USA. [Herdocia, K.; Valentin, V.] St Thomas Univ, Dept Biol, 16401 NW 37th Ave, Miami Gardens, FL 33054 USA. [Makinson, J.; Purcell, M. F.; Raghu, S.] USDA ARS, Australian Biol Control Lab, CSIRO, GPO Box 2583, Brisbane, Qld 4001, Australia. [Mattison, E.; Rayamajhi, M. B.] USDA ARS, Invas Plant Res Lab, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. RP Pratt, PD (reprint author), USDA ARS, Exot & Invas Weeds Res Unit, 800 Buchanan St, Albany, CA 94710 USA. EM Paul.Pratt@ars.usda.gov; kherdocia@stu.edu; vvalentin@stu.edu; Makinson@csiro.au; Matthew.Purcell@csiro.au; Elizabeth.Mattison@ars.usda.gov; Min.Raymajhi@ars.usda.gov; Patrick.Moran@ars.usda.gov; Raghu.Sathyamurthy@csiro.au FU St. Thomas University; USDA-HSI FX We thank two anonymous reviewers for comments on earlier versions of the manuscript. The authors thank the support of St. Thomas University and the USDA-HSI funded grant consortium Florida-Caribbean Consortium for Agriculture Education, its staff, and the USDA agency directors and supervisors. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity employer and provider. NR 34 TC 0 Z9 0 U1 2 U2 2 PU PACIFIC COAST ENTOMOL SOC PI SAN FRANCISCO PA C/O CALIFORNIA ACADEMY SCIENCES, 875 HOWARD STREET, SAN FRANCISCO, CA 94103-3009 USA SN 0031-0603 EI 2162-0237 J9 PAN-PAC ENTOMOL JI Pan-Pacific Entomol. PD OCT PY 2016 VL 92 IS 4 BP 200 EP 209 PG 10 WC Entomology SC Entomology GA EG3XD UT WOS:000390977100003 ER PT J AU Carvalho, LB Duke, SO Messa, JR Costa, FR Bianco, S AF Carvalho, L. B. Duke, S. O. Messa, J. R. Costa, F. R. Bianco, S. TI PLANT GROWTH RESPONSES OF APPLE AND PEAR TREES TO DOSES OF GLYPHOSATE SO PLANTA DANINHA LA English DT Article DE N-phosphonomethyl-glycine; Malus domestica; Pyrus communis; spray drift ID RYEGRASS LOLIUM-MULTIFLORUM; EPICUTICULAR WAX; RESISTANCE; TOLERANCE; MECHANISMS; ROOTSTOCKS; CULTIVARS; BIOTYPES; ARABICA; CROPS AB Glyphosate is commonly used for intra-row weed management in perennial plantations, where unintended crop exposure to this herbicide can cause growth reduction. The objective of this research was to analyze the initial plant growth behavior of young apple and pear plants exposed to glyphosate. Glyphosate was sprayed on 2-year-old 'Gala' apple and 'Abbe Fetel' pear plants at doses from 18 to 720 g per hectare of acid equivalent (a.e.). The plant height of neither species was not significantly reduced (less than 1%) by any glyphosate dose at 240 days after spraying, whereas the stem diameter and the dry mass of stem and leaves were reduced by 720 g a.e. ha(-1). The glyphosate dose required to reduce the aboveground dry mass by 50% was 162 and 148 g a.e. ha-1 for apple and pear, respectively. Aboveground dry mass was reduced 2% and 6% for apple and pear plants, respectively, at 720 g a.e. ha(-1). Hormesis was not observed in either species at doses down to 18 g a.e. ha(-1). Both species showed low susceptibility to glyphosate; however apple was less susceptible than pear. C1 [Carvalho, L. B.; Messa, J. R.; Costa, F. R.] Univ Estado Santa Catarina, Lages, SC, Brazil. [Duke, S. O.] Univ Mississippi, USDA, Oxford, MS USA. [Bianco, S.] Univ Estadual Paulista, Jaboticabal, SP, Brazil. RP Carvalho, LB (reprint author), Univ Estado Santa Catarina, Lages, SC, Brazil. EM lbcarvalho@dracena.unesp.br FU CNPq (PIBIC/CNPq Scholarship Program) from the Brazilian Government; CAPES (Master Scholarship Program) from the Brazilian Government FX The authors thank CNPq (PIBIC/CNPq Scholarship Program) and CAPES (Master Scholarship Program) from the Brazilian Government for supporting this research. NR 42 TC 0 Z9 0 U1 9 U2 9 PU UNIV FEDERAL VICOSA PI VICOSA PA CAIXA POSTAL 270, VICOSA, MG CEP 36571-00, BRAZIL SN 0100-8358 EI 1806-9681 J9 PLANTA DANINHA JI Planta Daninha PD OCT-DEC PY 2016 VL 34 IS 4 BP 815 EP 822 DI 10.1590/S0100-83582016340400022 PG 8 WC Plant Sciences SC Plant Sciences GA EF6JE UT WOS:000390436500022 ER PT J AU Miyazawa, Y Dudley, BD Hughes, RF Vandemark, J Cordell, S Nullet, MA Ostertag, R Giambelluca, TW AF Miyazawa, Yoshiyuki Dudley, Bruce D. Hughes, R. Flint Vandemark, Joshua Cordell, Susan Nullet, Michael A. Ostertag, Rebecca Giambelluca, Thomas W. TI Non-native tree in a dry coastal area in Hawai'i has high transpiration but restricts water use despite phreatophytic trait SO ECOHYDROLOGY LA English DT Article DE coastal groundwater; carbon assimilation; invasive species; sap flow; stomatal control ID SAP FLOW MEASUREMENTS; STOMATAL CONDUCTANCE; PHOTOSYNTHETIC CAPACITY; EUCALYPTUS-GLOBULUS; SEASONAL-VARIATION; CO2 ASSIMILATION; PROSOPIS-PALLIDA; HYDRAULIC LIMITS; VEGETATION TYPES; CARBON-DIOXIDE AB In arid systems, phreatophytes are often among the most effective invaders because of their capacity to access and exploit groundwater resources otherwise unavailable to native vegetation. On Hawai'i Island, a non-native phreatophyte, Prosopis pallida (kiawe), has invaded extensive dry lowland areas following its introduction in the 19th century. To better understand the influence of this invader on the host ecosystem, and vice versa, we determined transpiration characteristics of P. pallida by monitoring sap flux of mature individuals over an 18-month period on the arid leeward coast of Hawai'i Island. Daily sap flux increased with increasing atmospheric vapour pressure deficit (D) and exhibited no clear response related to rainfall events or prolonged drought. Annual transpiration (i.e. 308 mm) was 80% higher than rainfall. Stomatal conductance decreased with increasing vapour pressure deficits more slowly than the theoretical stomatal conductance-D relationship that assumes stomatal regulation of leaf water potential above a critical level. While daily peak stomatal conductance was relatively high, it nonetheless appeared limited by high intrinsic water use efficiency (net photosynthetic rate per stomatal conductance), suggesting a constraint to unlimited groundwater absorption. In this young volcanic environment where rainfall is low and quite episodic, the success of this species likely stems from its anisohydric strategy even at the risk of cavitation, and it is altering the hydrological cycling of the areas where it is dominant. Copyright (C) 2015 John Wiley & Sons, Ltd. C1 [Miyazawa, Yoshiyuki; Nullet, Michael A.; Giambelluca, Thomas W.] Univ Hawaii Manoa, Dept Geog, Honolulu, HI 96822 USA. [Miyazawa, Yoshiyuki] Kyushu Univ, Res Inst East Asia Environm, 744 Motooka, Fukuoka 8190395, Japan. [Dudley, Bruce D.; Hughes, R. Flint; Vandemark, Joshua; Cordell, Susan] US Forest Serv, Inst Pacific Isl Forestry, USDA, 60 Nowelo St, Hilo, HI 96720 USA. [Dudley, Bruce D.; Vandemark, Joshua; Ostertag, Rebecca] Univ Hawaii, Dept Biol, 200 W Kawili St, Hilo, HI 96720 USA. [Dudley, Bruce D.] Natl Inst Water & Atmospher Res, 10 Kyle St, Christchurch, New Zealand. RP Miyazawa, Y (reprint author), Kyushu Univ, Res Inst East Asia Environm, 744 Motooka, Fukuoka 8190395, Japan. EM sclerophyll@gmail.com OI Giambelluca, Thomas/0000-0002-6798-3780 FU NSF - Hawai'i EPSCoR [EPS-0903833]; USDA Forest Service Pacific Southwest Research Station; JSPS [20-7278]; Excellent Young Researchers Overseas Visit Program for research at the University of Hawai'i at Manoa FX We thank Dr Creighton M. Litton (University of Hawai'i at Manoa) for allowing us to use instruments for field measurements, Hui Aloha Kiholo community group for their support of this research and the Hawai'i Department of Land and Natural Resources staff for providing access to Kiholo State Park. This study was supported by funding from NSF - Hawai'i EPSCoR grant no. EPS-0903833 and received in-kind support from the USDA Forest Service Pacific Southwest Research Station. This study was partly supported by the JSPS to Y. Miyazawa (20-7278) and Excellent Young Researchers Overseas Visit Program for research at the University of Hawai'i at Manoa. NR 64 TC 0 Z9 0 U1 4 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1936-0584 EI 1936-0592 J9 ECOHYDROLOGY JI Ecohydrology PD OCT PY 2016 VL 9 IS 7 BP 1166 EP 1176 DI 10.1002/eco.1715 PG 11 WC Ecology; Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA EF0NF UT WOS:000390021700003 ER PT J AU Lopez-Martinez, G Meagher, RL Jeffers, LA Bailey, WD Hahn, DA AF Lopez-Martinez, Giancarlo Meagher, Robert L. Jeffers, Laura A. Bailey, Woodward D. Hahn, Daniel A. TI Low oxygen atmosphere enhances post-irradiation survival of Trichoplusia ni (Lepidoptera: Noctuidae) SO FLORIDA ENTOMOLOGIST LA English DT Article DE cross-tolerance; fertility; oxygen-effect; phytosanitary irradiation; radiotolerance ID FLY DIPTERA TEPHRITIDAE; IONIZING-RADIATION; FRUIT-FLY; PHYTOSANITARY TREATMENTS; GAMMA-IRRADIATION; INDUCED STERILITY; MOTH LEPIDOPTERA; NITROGEN; MALES; PUPAE AB Phytosanitary irradiation (PI) is increasingly being used for disinfesting fresh commodities from insect pests of quarantine concern. The development of generic doses of irradiation that can be applied to broadly control all pests within a particular family, order, or even all insects across commodities and packaging types could facilitate greater use of PI. Many commodities are stored in controlled or modified atmospheres that are low in oxygen to preserve commodity quality and extend shelf life, but low-oxygen environments have been shown to affect radiotolerance in some insects and more work is needed to understand the impact of this oxygen-effect on radiotolerance for both the development of generic doses and to increase the acceptance of irradiation as a treatment by the fresh commodity industry. Here we show that irradiation of cabbage looper, Trichoplusia ni (Hubner) (Lepidoptera: Noctuidae), in anoxic atmospheres (0 kPa aPO(2)) increases radiotolerance compared to irradiation in normoxic atmospheres (21 kPa aPO(2)) last instar larvae, true pupae and mature pupae (pharate adults) when considering the emergence of healthy-looking, viable adults as the critical metric. Of the 3 stages irradiated, the last instar larvae were most susceptible to irradiation, followed by pupae, with pharate adults 24-48 h prior to emergence (sometimes referred to as late pupae) being the most tolerant stage. When pharate adults were irradiated in anoxia, healthy-looking adults emerged at absorbed doses of 784-789 Gy whereas no healthy-looking adults emerged at the same doses in normoxia. Effects of anoxia on reproduction of irradiated female pharate adults were subtle. A few F-1 larvae hatched at doses estimated to be 585-591 Gy, suggesting that more work is needed to determine whether 400 Gy is an adequate generic dose to control late pupae of T. ni. C1 [Lopez-Martinez, Giancarlo; Hahn, Daniel A.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Lopez-Martinez, Giancarlo] New Mexico State Univ, Dept Biol, Las Cruces, NM 88003 USA. [Meagher, Robert L.] USDA ARS, CMAVF, Gainesville, FL 32608 USA. [Jeffers, Laura A.] USDA, APHIS, PPQ Ctr Plant Hlth Sci & Technol, Raleigh, NC USA. [Bailey, Woodward D.] USDA, APHIS, PPQ Subtrop Quarantine Inspect, Coral Gables, FL USA. RP Lopez-Martinez, G (reprint author), Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA.; Lopez-Martinez, G (reprint author), New Mexico State Univ, Dept Biol, Las Cruces, NM 88003 USA. EM gclopez@nmsu.edu FU FAO/IAEA Coordinated Research Project on Development of Generic Irradiation Doses for Quarantine Treatments [D62008]; USDA-APHIS [13-8130-0159-CA]; Florida Agricultural Experiment Station FX This work was part of the FAO/IAEA Coordinated Research Project D62008 on Development of Generic Irradiation Doses for Quarantine Treatments. The research was funded by USDA-APHIS 13-8130-0159-CA to DAH and supported by the Florida Agricultural Experiment Station. The authors wish to thank Carl Gillis from the Florida Department of Agriculture and Consumer Services (FDACS-DPI) for providing us irradiation assistance and Andrea Beam USDA-APHIS-Miami and Catriona Condon UFL for help with alanine dosimetry. We thank Amy Rowley for rearing assistance and advice on the construction of the mating cages, Lyle Buss for taking photographs of our treated moths, and Sabrina White and Theodore Cogley for help in setup, husbandry of treated animals, and data collection. An IAEA/FAO Coordinated Research Project on Development of Generic Irradiation Doses for Quarantine Treatments supported this work intellectually. Our research has benefited greatly from advice and support from members of the above group, and additional comments on this manuscript from Andrew Parker and Guy Hallman were most appreciated. NR 32 TC 1 Z9 1 U1 2 U2 2 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD OCT PY 2016 VL 99 SI 2 BP 24 EP 33 PG 10 WC Entomology SC Entomology GA EF2HF UT WOS:000390144700004 ER PT J AU Hallman, GJ Chapa, DL AF Hallman, Guy J. Chapa, Deanna L. TI Phytosanitary Irradiation of Diaphorina citri (Hemiptera: Liviidae) SO FLORIDA ENTOMOLOGIST LA English DT Article DE citrus greening disease; huanglongbing; acute lethal dose; prevent disease transmission; radiotolerance; sterilizing dose ID DISEASE AB Asian citrus psyllid (Diaphorina citri Kuwayama; Hemiptera: Liviidae) adults reared on orange jasmine (Murraya paniculata (L.) Jack; Sapindales: Rutaceae) were evaluated for acute mortality and prevention of reproduction when adult insects were irradiated for phytosanitary purposes. At 2 kGy all adults (n = 60) were dead by 72 h post irradiation. A dose of 150 Gy did not prevent oviposition (n = 1,200 adults), but no nymphs were observed to emerge from the eggs. At the next lowest dose (100 Gy) some nymphs developed. These results indicate that a minimum PI treatment dose significantly greater than 1.5 kGy but less than 2 kGy is required for total mortality within 72 h and the minimum PI treatment dose to prevent egg hatch is greater than 100 Gy, but less than 150 Gy. C1 [Hallman, Guy J.] ARS, USDA, Ctr Grain & Anim Hlth Res, Stored Prod Insect Res Unit, 1515 Coll Ave, Manhattan, KS 66502 USA. [Chapa, Deanna L.] ARS, USDA, Anim Parasit Dis Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Hallman, GJ (reprint author), ARS, USDA, Ctr Grain & Anim Hlth Res, Stored Prod Insect Res Unit, 1515 Coll Ave, Manhattan, KS 66502 USA. EM G.J.Hallman@iaea.org FU FAO/IAEA Coordinated Research Project [D62008] FX Maura Rodriguez, Roberto Rivas, Faith Banda, Elvia Landero, Yadira Blanco and Marc Villalobos formerly of USDA-ARS, Weslaco, Texas are thanked for technical help. USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. This work was part of the FAO/IAEA Coordinated Research Project D62008 on Development of Generic Irradiation Doses for Quarantine Treatments. NR 14 TC 3 Z9 3 U1 0 U2 0 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD OCT PY 2016 VL 99 SI 2 BP 150 EP 152 PG 3 WC Entomology SC Entomology GA EF2HF UT WOS:000390144700024 ER PT J AU Hallman, GJ AF Hallman, Guy J. TI Phytosanitary irradiation of the invasive herbivorous terrestrial snail Cornu aspersum (Stylommatophora: Helicidae) SO FLORIDA ENTOMOLOGIST LA English DT Article DE escargot; invasive species; prevention of reproduction; radiosensitivity; regulated articles ID AUSTRALORBIS GLABRATUS; POPULATION KINETICS; IONIZING-RADIATION; EXPOSURE; AGE AB Phytosanitary measures are needed to prevent the transport of snails and slugs during trade in regulated articles. Ionizing radiation is increasing in use as a phytosanitary treatment against insect pests. Irradiation has the potential to be a treatment against snails and slugs, but little research has been done on radiosensitivity of terrestrial herbivorous gastropods. Adult Cornu aspersum (Muller) (Stylommatophora: Helicidae) were subjected to ionizing radiation at 25-100 Gy. Irradiation decreased the number of eggs laid, and those laid by adults irradiated with >= 75 Gy did not hatch. This research suggests that gastropod reproduction can be prevented with relatively low doses of radiation. A phytosanitary irradiation dose for C. aspersum should also serve to allow export of live snail species as food to areas that quarantine against them. C1 [Hallman, Guy J.] ARS, USDA, Ctr Grain & Anim Hlth Res, Stored Prod Insect Res Unit, 1515 Coll Ave, Manhattan, KS 66502 USA. [Hallman, Guy J.] Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Lab, Vienna, Austria. RP Hallman, GJ (reprint author), ARS, USDA, Ctr Grain & Anim Hlth Res, Stored Prod Insect Res Unit, 1515 Coll Ave, Manhattan, KS 66502 USA.; Hallman, GJ (reprint author), Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Lab, Vienna, Austria. EM G.J.Hallman@iaea.org FU FAO/IAEA Coordinated Research Project [D62008] FX Beth Grafton-Cardwell and Janet McLain, Univ. of California, Kearney, are thanked for providing C. aspersum. Crissy Gutierrez and Maura Rodriguez, formerly with USDA-ARS, Weslaco, TX, are thanked for technical support. John Diaz-Montano, USDA-ARS, Manhattan, KS, is thanked for correcting the resumen. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. USDA is an equal opportunity provider and employer. This work was part of the FAO/IAEA Coordinated Research Project D62008 on Development of Generic Irradiation Doses for Quarantine Treatments. NR 15 TC 1 Z9 1 U1 5 U2 5 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD OCT PY 2016 VL 99 SI 2 BP 156 EP 158 PG 3 WC Entomology SC Entomology GA EF2HF UT WOS:000390144700026 ER PT J AU Hallman, GJ AF Hallman, Guy J. TI Phytosanitary Irradiation of Heliothis virescens and Helicoverpa zea (Lepidoptera: Noctuidae) SO FLORIDA ENTOMOLOGIST LA English DT Article DE radiation; quarantine treatment; tobacco budworm; corn earworm ID IONIZING-RADIATION; QUARANTINE TREATMENT; TORTRICIDAE; DIPTERA; AMBIENT; LARVAE; EGGS AB Phytosanitary irradiation is being increasingly used as a phytosanitary treatment to disinfest fresh commodities of invasive species. Optimum irradiation treatment doses for a number of invasive species groups, such as the family Noctuidae, are required. This research was designed to provide data for developing an optimum generic irradiation dose suitable against all eggs and larvae of the Noctuidae. Last instars (the most radiotolerant stage normally found on exported commodities) of 2 noctuid species were studied, i.e., Heliothis virescens (F.) and Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae). There was no significant difference in emergence of normal-looking adults from H. virescens last instars reared on either a natural or an artificial host, i.e., mature pods of Phaseolus vulgaris L. (Fabales: Fabaceae) or a meridic diet, respectively. Two laboratory colonies of H. virescens were studied, one appeared to be more radiotolerant than the other and it was used for large-scale confirmatory testing of a putative phytosanitary irradiation dose treatment. A total of 14,366 last instars were irradiated with 150 Gy (actual measured dose distribution was 145-166 Gy) with none emerging as normal-looking adults. A total of 2,197 last instar H. zea reared on meridic diet were irradiated with the same dose and no normal-looking adults emerged. This research indicates that a treatment dose of approximately 150 Gy is sufficient as a phytosanitary treatment for these 2 noctuid species and adds to the body of research evidence that a generic dose treatment of 250 Gy is adequate to control eggs and larvae of Lepidoptera that infest fresh commodities. C1 [Hallman, Guy J.] ARS, USDA, Weslaco, TX 78596 USA. [Hallman, Guy J.] Vienna Int Ctr, Joint FAO IAEA Programme, Nucl Tech Food & Agr, Vienna, Austria. RP Hallman, GJ (reprint author), ARS, USDA, Weslaco, TX 78596 USA.; Hallman, GJ (reprint author), Vienna Int Ctr, Joint FAO IAEA Programme, Nucl Tech Food & Agr, Vienna, Austria. EM G.J.Hallman@iaea.org FU FAO/IAEA [D62008] FX Carlos Blanco, USDA-APHIS, is thanked for providing insects when he was formerly employed by USDA-ARS in Stoneville, Mississippi, USA. This work was part of the FAO/IAEA Coordinated Research Project D62008 on Development of Generic Irradiation Doses for Quarantine Treatments. NR 22 TC 2 Z9 2 U1 0 U2 0 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD OCT PY 2016 VL 99 SI 2 BP 178 EP 181 PG 4 WC Entomology SC Entomology GA EF2HF UT WOS:000390144700030 ER PT J AU Hallman, GJ Legaspi, JC Darmawi AF Hallman, Guy J. Legaspi, Jesusa Crisostomo Darmawi TI Phytosanitary irradiation of Diatraea saccharalis, D. grandiosella, and Eoreuma loftini (Lepidoptera: Crambidae) SO FLORIDA ENTOMOLOGIST LA English DT Article DE generic treatment dose; ionizing radiation; quarantine treatment; invasive species; phytosanitation; Diaprepes abbreviatus ID GY AB Phytosanitary irradiation (PI) is increasingly being used to disinfest horticultural commodities of invasive quarantine pests. Most disinfestation is done with generic treatments, where the same dose is applied against a group of pest species and/or commodities. The current generic treatment used by the Animal and Plant Health Inspection Service of the United States Department of Agriculture (USDA-APHIS) against Class Insecta-i.e., 400 Gy-does not include pupae of any species of Lepidoptera. More data are needed to develop a generic dose for those Lepidoptera that pupate on or within shipped commodities, such as Crambidae species. Tests were done with the crambids Diatraea grandiosella Dyar, D. saccharalis (F.), and Eoreuma loftini (Dyar) (Lepidoptera: Crambidae) to seek doses that would provide quarantine security no only against late pupae but also against last instar larvae, and that would possibly contribute to the development of a generic PI dose that is less than the current 400 Gy. The results of this research support proposed generic doses of 250 and 400 Gy for larvae and pupae of Lepidoptera species, respectively, and suggest lower specific doses for the individual insect species. C1 [Hallman, Guy J.] IAEA, Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Lab, Vienna, Austria. [Legaspi, Jesusa Crisostomo] Florida A&M Univ, USDA, ARS, CMAVE,Ctr Biol Control, 6383 Mahan Dr, Tallahassee, FL 32608 USA. [Darmawi] CAIR BATAN, Jakarta, Indonesia. RP Hallman, GJ (reprint author), IAEA, Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Lab, Vienna, Austria. EM g.j.hallman@iaea.org FU FAO/IAEA Coordinated Research Project on Development of Generic Irradiation Doses for Quarantine Treatments [D62008] FX This work was part of the FAO/IAEA Coordinated Research Project D62008 on Development of Generic Irradiation Doses for Quarantine Treatments. NR 15 TC 0 Z9 0 U1 0 U2 0 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD OCT PY 2016 VL 99 SI 2 BP 182 EP 185 PG 4 WC Entomology SC Entomology GA EF2HF UT WOS:000390144700031 ER PT J AU Marshall, CM Tartaglio, V Duarte, M Harmon, FG AF Marshall, Carine M. Tartaglio, Virginia Duarte, Maritza Harmon, Frank G. TI The Arabidopsis sickle Mutant Exhibits Altered Circadian Clock Responses to Cool Temperatures and Temperature-Dependent Alternative Splicing SO PLANT CELL LA English DT Article ID MESSENGER-RNA DECAY; TRANSCRIPTION FACTOR; HIGH-THROUGHPUT; PLANT; PROTEIN; GENES; THALIANA; NONSENSE; EXPRESSION; RHYTHMS AB The circadian clock allows plants to anticipate and respond to daily changes in ambient temperature. Mechanisms establishing the timing of circadian rhythms in Arabidopsis thaliana through temperature entrainment remain unclear. Also incompletely understood is the temperature compensation mechanism that maintains consistent period length within a range of ambient temperatures. A genetic screen for Arabidopsis mutants affecting temperature regulation of the PSEUDO-RESPONSE REGULATOR7 promoter yielded a novel allele of the SICKLE (SIC) gene. This mutant, sic-3, and the existing sic-1 mutant both exhibit low-amplitude or arrhythmic expression of core circadian clock genes under cool ambient temperature cycles, but not under light-dark entrainment. sic mutants also lengthen free running period in a manner consistent with impaired temperature compensation. sic mutant alleles accumulate LATE ELONGATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK ASSOCIATED1 (CCA1) splice variants, among other alternatively spliced transcripts, which is exacerbated by cool temperatures. The cca1-1 lhy-20 double mutant is epistatic to sic-3, indicating the LHY and CCA1 splice variants are needed for sic-3 circadian clock phenotypes. It is not expected that SIC is directly involved in the circadian clock mechanism; instead, SIC likely contributes to pre-mRNA metabolism, and the splice variants that accumulate in sic mutants likely affect the circadian clock response to cool ambient temperature. C1 [Marshall, Carine M.; Tartaglio, Virginia; Duarte, Maritza; Harmon, Frank G.] USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA. [Marshall, Carine M.; Tartaglio, Virginia; Duarte, Maritza; Harmon, Frank G.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. RP Harmon, FG (reprint author), USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA.; Harmon, FG (reprint author), Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. EM fharmon@berkeley.edu OI Marshall, Carine/0000-0001-7178-4664 FU USDA [2030-21000-039-00D]; National Science Foundation [IOS1238048]; University of California, Berkeley FX We are thank C. Robertson McClung (Dartmouth College) for the ProPRR7: LUC reporter line, Bryan Thines (University of California, Berkeley) for creating the EMS-mutagenized population, Michael Covington (University of California, Davis) for sharing his polar plot script for R, and Michael Koontz (University of California, Davis) for help modifying the polar plot script. We thank Riva Bruenn (University of California, Berkeley) for her advice and experience in phylogenetic analysis. We thank Mark Mullan, Cody Schaaf, and Samantha Nguyen for research assistance. We thank University of California, Berkeley Summer Undergraduate Fellowships/Rose Hills for supporting Mark Mullan. We thank Claire Bendix, Emma Kovak, Dominica Rohozinski, Dominik Brilhaus, and Achim Werner for valuable feedback on early drafts of the manuscript. This work is supported by the USDA (2030-21000-039-00D to F.G.H.) and by the National Science Foundation (IOS1238048 to F.G.H.). NR 89 TC 3 Z9 3 U1 7 U2 7 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD OCT PY 2016 VL 28 IS 10 BP 2560 EP 2575 DI 10.1105/tpc.16.00223 PG 16 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA EF2DR UT WOS:000390135400019 PM 27624757 ER PT J AU Kumar, S Kempinski, C Zhuang, X Norris, A Mafu, S Zi, JC Bell, SA Nybo, SE Kinison, SE Jiang, ZD Goklany, S Linscott, KB Chen, XL Jia, QD Brown, SD Bowman, JL Babbitt, PC Peters, RJ Chen, F Chappell, J AF Kumar, Santosh Kempinski, Chase Zhuang, Xun Norris, Ayla Mafu, Sibongile Zi, Jiachen Bell, Stephen A. Nybo, Stephen Eric Kinison, Scott E. Jiang, Zuodong Goklany, Sheba Linscott, Kristin B. Chen, Xinlu Jia, Qidong Brown, Shoshana D. Bowman, John L. Babbitt, Patricia C. Peters, Reuben J. Chen, Feng Chappell, Joe TI Molecular Diversity of Terpene Synthases in the Liverwort Marchantia polymorpha SO PLANT CELL LA English DT Article ID ENT-KAURENE SYNTHASE; MOSS PHYSCOMITRELLA-PATENS; BIFUNCTIONAL DITERPENE CYCLASE; COPALYL DIPHOSPHATE SYNTHASE; FUNCTION LINKAGE DATABASE; PRE-STEADY-STATE; LAND PLANTS; FUNCTIONAL-CHARACTERIZATION; GIBBERELLIN BIOSYNTHESIS; NATURAL-PRODUCTS AB Marchantia polymorpha is a basal terrestrial land plant, which like most liverworts accumulates structurally diverse terpenes believed to serve in deterring disease and herbivory. Previous studies have suggested that the mevalonate and methylerythritol phosphate pathways, present in evolutionarily diverged plants, are also operative in liverworts. However, the genes and enzymes responsible for the chemical diversity of terpenes have yet to be described. In this study, we resorted to a HMMER search tool to identify 17 putative terpene synthase genes from M. polymorpha transcriptomes. Functional characterization identified four diterpene synthase genes phylogenetically related to those found in diverged plants and nine rather unusual monoterpene and sesquiterpene synthase-like genes. The presence of separate monofunctional diterpene synthases for ent-copalyl diphosphate and ent-kaurene biosynthesis is similar to orthologs found in vascular plants, pushing the date of the underlying gene duplication and neofunctionalization of the ancestral diterpene synthase gene family to >400 million years ago. By contrast, the mono-and sesquiterpene synthases represent a distinct class of enzymes, not related to previously described plant terpene synthases and only distantly so to microbial-type terpene synthases. The absence of a Mg2+ binding, aspartate-rich, DDXXD motif places these enzymes in a noncanonical family of terpene synthases. C1 [Kumar, Santosh; Kempinski, Chase; Zhuang, Xun; Bell, Stephen A.; Nybo, Stephen Eric; Kinison, Scott E.; Jiang, Zuodong; Goklany, Sheba; Linscott, Kristin B.; Chappell, Joe] Univ Kentucky, Plant Biol Program, Lexington, KY 40536 USA. [Kumar, Santosh; Kempinski, Chase; Zhuang, Xun; Bell, Stephen A.; Nybo, Stephen Eric; Kinison, Scott E.; Jiang, Zuodong; Goklany, Sheba; Linscott, Kristin B.; Chappell, Joe] Univ Kentucky, Dept Pharmaceut Sci, Lexington, KY 40536 USA. [Norris, Ayla; Jia, Qidong; Chen, Feng] Univ Tennessee, Gradaute Sch Genome Sci & Technol, Knoxville, TN 37996 USA. [Mafu, Sibongile; Zi, Jiachen; Peters, Reuben J.] Iowa State Univ, Roy J Carver Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA. [Chen, Xinlu; Chen, Feng] Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA. [Brown, Shoshana D.; Babbitt, Patricia C.] Univ Calif San Francisco, Calif Inst Quantitat Biosci, Dept Bioengn, San Francisco, CA 94158 USA. [Brown, Shoshana D.; Babbitt, Patricia C.] Univ Calif San Francisco, Calif Inst Quantitat Biosci, Dept Therapeut Sci & Pharmaceut Chem, San Francisco, CA 94158 USA. [Bowman, John L.] Monash Univ, Sch Biol Sci, Melbourne, Vic 3800, Australia. [Zhuang, Xun] Sandia Natl Labs, Livermore, CA 94551 USA. [Norris, Ayla] USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. [Mafu, Sibongile] Univ Calif Davis, Dept Plant Biol, Davis, CA 95616 USA. [Zi, Jiachen] Jinan Univ, Biotechnol Inst Chinese Mat Med, Guangzhou 510632, Guangdong, Peoples R China. [Bell, Stephen A.] Univ Utah, Dept Med Chem, Salt Lake City, UT 84112 USA. [Nybo, Stephen Eric] Ferris State Univ, Med Chem, Coll Pharm, Big Rapids, MI 49307 USA. [Goklany, Sheba] Arizona State Univ, Sch Engn Matter Transport & Energy, Tempe, AZ 85287 USA. RP Chappell, J (reprint author), Univ Kentucky, Plant Biol Program, Lexington, KY 40536 USA.; Chappell, J (reprint author), Univ Kentucky, Dept Pharmaceut Sci, Lexington, KY 40536 USA. EM chappell@uky.edu RI Peters, Reuben/D-1196-2010; OI Peters, Reuben/0000-0003-4691-8477; Chen, Xinlu/0000-0002-7560-6125 FU National Institutes of Health [1RC2GM092521, GM076324, GM60595]; University of Tennessee Institute of Agriculture; Australian Research Council [DP160100892]; Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231] FX We thank Tobias G. Kollner and Jonathan Gershenzon for their contributions in the early stage of this study. This work was supported, in part, by Grants 1RC2GM092521 from the National Institutes of Health to JC, GM076324 from the National Institutes of Health to RJP, an Innovation Grant from the University of Tennessee Institute of Agriculture to F.C., GM60595 from the National Institutes of Health to P.C.B., and DP160100892 from the Australian Research Council to J.L.B. We also thank our colleagues at Joint Genome Institute for access to the M. polymorpha genome sequence information, work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, supported by the Office of Science of the U.S. Department of Energy under Contract DE-AC02-05CH11231. NR 88 TC 0 Z9 0 U1 10 U2 10 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD OCT PY 2016 VL 28 IS 10 BP 2632 EP 2650 DI 10.1105/tpc.16.00062 PG 19 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA EF2DR UT WOS:000390135400024 PM 27650333 ER PT J AU Richter, A Schaff, C Zhang, ZW Lipka, AE Tian, F Kollner, TG Schnee, C Preiss, S Irmisch, S Jander, G Boland, W Gershenzon, J Buckler, ES Degenhardt, J AF Richter, Annett Schaff, Claudia Zhang, Zhiwu Lipka, Alexander E. Tian, Feng Kollner, Tobias G. Schnee, Christiane Preiss, Susanne Irmisch, Sandra Jander, Georg Boland, Willhelm Gershenzon, Jonathan Buckler, Edward S. Degenhardt, Jorg TI Characterization of Biosynthetic Pathways for the Production of the Volatile Homoterpenes DMNT and TMTT in Zea mays SO PLANT CELL LA English DT Article ID ASSOCIATION MAPPING POPULATION; GENOME-WIDE ASSOCIATION; INDUCED PLANT VOLATILES; ISOPRENOID BIOSYNTHESIS; FARNESYL DIPHOSPHATE; GENETIC ARCHITECTURE; MEDICAGO-TRUNCATULA; HERBIVORE ENEMIES; CHEMICAL ECOLOGY; INDIRECT DEFENSE AB Plant volatiles not only have multiple defense functions against herbivores, fungi, and bacteria, but also have been implicated in signaling within the plant and toward other organisms. Elucidating the function of individual plant volatiles will require more knowledge of their biosynthesis and regulation in response to external stimuli. By exploiting the variation of herbivore-induced volatiles among 26 maize (Zea mays) inbred lines, we conducted a nested association mapping and genome-wide association study (GWAS) to identify a set of quantitative trait loci (QTLs) for investigating the pathways of volatile terpene production. The most significant identified QTL affects the emission of (E)-nerolidol, linalool, and the two homoterpenes (E)-3,8-dimethyl-1,4,7-nonatriene (DMNT) and (E, E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene (TMTT). GWAS associated a single nucleotide polymorphism in the promoter of the gene encoding the terpene synthase TPS2 with this QTL. Biochemical characterization of TPS2 verified that this plastid-localized enzyme forms linalool, (E)-nerolidol, and (E, E)-geranyllinalool. The subsequent conversion of (E)-nerolidol into DMNT maps to a P450 monooxygenase, CYP92C5, which is capable of converting nerolidol into DMNT by oxidative degradation. A QTL influencing TMTT accumulation corresponds to a similar monooxygenase, CYP92C6, which is specific for the conversion of (E, E)-geranyllinalool to TMTT. The DMNT biosynthetic pathway and both monooxygenases are distinct from those previously characterized for DMNT and TMTT synthesis in Arabidopsis thaliana, suggesting independent evolution of these enzymatic activities. C1 [Richter, Annett; Schaff, Claudia; Preiss, Susanne; Degenhardt, Jorg] Martin Luther Univ Halle Wittenberg, Inst Pharm, D-06120 Halle, Germany. [Zhang, Zhiwu; Lipka, Alexander E.; Tian, Feng; Buckler, Edward S.] Cornell Univ, Inst Genom Divers, Ithaca, NY 14853 USA. [Kollner, Tobias G.; Schnee, Christiane; Irmisch, Sandra; Boland, Willhelm; Gershenzon, Jonathan] Max Planck Inst Chem Ecol, D-07745 Jena, Germany. [Richter, Annett; Jander, Georg] Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Buckler, Edward S.] USDA ARS, Ithaca, NY 14853 USA. [Zhang, Zhiwu] Washington State Univ, Dept Crops & Soil Sci, Pullman, WA 99164 USA. [Lipka, Alexander E.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Tian, Feng] China Agr Univ, Natl Maize Improvement Ctr China, Beijing 100193, Peoples R China. [Irmisch, Sandra] Univ British Columbia, Vancouver, BC V6T 1Z4, Canada. RP Degenhardt, J (reprint author), Martin Luther Univ Halle Wittenberg, Inst Pharm, D-06120 Halle, Germany. EM joerg.degenhardt@pharmazie.uni-halle.de RI Gershenzon, Jonathan/K-1331-2013; Boland, Wilhelm/K-7762-2012; OI Gershenzon, Jonathan/0000-0002-1812-1551; Kollner, Tobias/0000-0002-7037-904X FU Collaborative Research Center 648 of the German Research Foundation (DFG); U.S. National Science Foundation [IOS-0820619, IOS-1238014, IOS-1139329, IOS-1339237]; USDA-Agricultural Research Service FX Wethank Jens Wurlitzer for plant collection, Michael Gore for processing of samples from the field, and Peter Lindemann for help with enzyme characterization. Wealso thank KWS Seeds for maize B73 seeds. A.R. and J.D. were supported by project B7 of the Collaborative Research Center 648 of the German Research Foundation (DFG). Germplasm, field, and mapping analysis were supported by grants from the U.S. National Science Foundation (IOS-0820619 and IOS-1238014) and the USDA-Agricultural Research Service. G.J. was supported by U.S. National Science Foundation Awards IOS-1139329 and IOS-1339237. NR 78 TC 1 Z9 1 U1 9 U2 9 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD OCT PY 2016 VL 28 IS 10 BP 2651 EP 2665 DI 10.1105/tpc.15.00919 PG 15 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA EF2DR UT WOS:000390135400025 PM 27662898 ER PT J AU Djanibekov, U Villamor, GB Dzhakypbekova, K Chamberlain, J Xu, JC AF Djanibekov, Utkur Villamor, Grace B. Dzhakypbekova, Klara Chamberlain, James Xu, Jianchu TI Adoption of Sustainable Land Uses in Post-Soviet Central Asia: The Case for Agroforestry SO SUSTAINABILITY LA English DT Article DE agroforestry; coevolution; sustainable land use; socio-ecological systems; transition countries ID AMU-DARYA; CLIMATE-CHANGE; UZBEKISTAN; SYSTEMS; PRODUCTIVITY; AGRICULTURE; PERFORMANCE; GOVERNANCE; SERVICES; IMPACTS AB We examine constraints and opportunities to enhance adoption of agroforestry for ecosystem and livelihood improvement in post-Soviet economies, using Central Asian countries as examples. Using a coevolutionary socio-ecological systems framework, we describe how development efforts, especially agricultural policies, under centrally planned regimes and under transition to market economies have changed environmental conditions, and how they affect peoples' welfare. We then discuss agroforestry as a sustainable land use practice to address these issues. We present regional-specific and -suitable agroforestry practices, and discuss their potential. We found that legacies from the previous period of central planning shape current land uses, institutions, infrastructure and decisions of farmers, and constrain development of agroforestry. By identifying opportunities and constraints of agroforestry, we provide recommendations for enhancing the use of agroforestry in Central Asia. C1 [Djanibekov, Utkur] Univ Bonn, Inst Food & Resource Econ ILR, Meckenheimer Allee 174, D-53115 Bonn, Germany. [Villamor, Grace B.] Univ Bonn, Ctr Dev Res ZEF, Walter Flex Str 3, D-53113 Bonn, Germany. [Dzhakypbekova, Klara] Univ Bonn, Agr Sci & Resource Management Trop & Subtrop, Nussallee 1, D-53115 Bonn, Germany. [Chamberlain, James] US Forest Serv, Southern Res Stn, FIA, 1710 Res Ctr Dr, Blacksburg, VA 24060 USA. [Xu, Jianchu] Kunming Inst Bot, World Agroforestry Ctr East & Cent Asia, Kunming 650201, Peoples R China. RP Djanibekov, U (reprint author), Univ Bonn, Inst Food & Resource Econ ILR, Meckenheimer Allee 174, D-53115 Bonn, Germany. EM u.djanibekov@ilr.uni-bonn.de; gracev@uni-bonn.de; klara.dzhakypbekova@uni-bonn.de; jchamberlain@fs.fed.us; j.c.xu@cgiar.org NR 94 TC 0 Z9 0 U1 3 U2 3 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2071-1050 J9 SUSTAINABILITY-BASEL JI Sustainability PD OCT PY 2016 VL 8 IS 10 AR 1030 DI 10.3390/su8101030 PG 16 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Environmental Sciences; Environmental Studies SC Science & Technology - Other Topics; Environmental Sciences & Ecology GA EE1BE UT WOS:000389314600070 ER PT J AU Tani, M Horvath, KV Lamarche, B Couture, P Burnett, JR Schaefer, EJ Asztalos, BF AF Tani, Mariko Horvath, Katalin V. Lamarche, Benoit Couture, Patrick Burnett, John R. Schaefer, Ernst J. Asztalos, Bela F. TI High-density lipoprotein subpopulation profiles in lipoprotein lipase and hepatic lipase deficiency SO ATHEROSCLEROSIS LA English DT Article DE Apolipoproteins; Lipoprotein metabolism; HDL particles; HDL remodeling; Reverse cholesterol transport ID APOLIPOPROTEIN-A-I; CORONARY-ARTERY-DISEASE; HDL SUBPOPULATIONS; APOA-I; ENDOTHELIAL LIPASE; HEART-DISEASE; METABOLISM; HUMANS; PLASMA; CHOLESTEROL AB Background and aims: Our aim was to gain insight into the role that lipoprotein lipase (LPL) and hepatic lipase (HL) plays in HDL metabolism and to better understand LPL- and HL-deficiency states. Methods: We examined the apolipoprotein (apo) A-I-, A-II-, A-IV-, C-I-, C-III-, and E-containing HDL subpopulation profiles, assessed by native 2-dimensional gel-electrophoresis and immunoblotting, in 6 homozygous and 11 heterozygous LPL- deficient, 6 homozygous and 4 heterozygous HL-deficient, and 50 control subjects. Results: LPL- deficient homozygotes had marked hypertriglyceridemia and significant decreases in LDL-C, HDL-C, and apoA-I. Their apoA-I-containing HDL subpopulation profile was shifted toward small HDL particles compared to controls. HL-deficient homozygotes had moderate hypertriglyceridemia, modest increases in LDL-C and HDL-C level, but normal apoA-I concentration. HL-deficient homozygotes had a unique distribution of apoA-I-containing HDL particles. The normally apoA-I: A-II, intermediate-size (alpha-2 and alpha-3) particles were significantly decreased, while the normally apoA-I only ( very large alpha-1, small alpha-4, and very small pre beta-1) particles were significantly elevated. In contrast to control subjects, the very large alpha-1 particles of HL-deficient homozygotes were enriched in apoA-II. Homozygous LPL- and HL-deficient subjects also had abnormal distributions of apo C-I, C-III, and E in HDL particles. Values for all measured parameters in LPL- and HL-deficient heterozygotes were closer to values measured in controls than in homozygotes. Conclusions: Our data are consistent with the concept that LPL is important for the maturation of small discoidal HDL particles into large spherical HDL particles, while HL is important for HDL remodeling of very large HDL particles into intermediate-size HDL particles. (C) 2016 Elsevier Ireland Ltd. All rights reserved. C1 [Tani, Mariko; Horvath, Katalin V.; Schaefer, Ernst J.; Asztalos, Bela F.] Tufts Univ, Human Nutr Res Ctr Aging, Cardiovasc Nutr Lab, Boston, MA 02111 USA. [Lamarche, Benoit; Couture, Patrick] Univ Laval, Inst Nutraceut & Funct Foods, Quebec City, PQ, Canada. [Burnett, John R.] Univ Western Australia, Royal Perth & Fiona Stanley Hosp Network, Path West Lab Med, Dept Clin Biochem, Perth, WA, Australia. [Burnett, John R.] Univ Western Australia, Sch Med & Pharmacol, Perth, WA, Australia. RP Asztalos, BF (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr, Res Ctr Aging, Cardiovasc Nutr Lab, 711 Washington St, Boston, MA 02111 USA. EM bela.asztalos@tufts.edu FU National Heart, Lung, and Blood Institute [HL117933]; US Department of Agriculture [53-3K06-5-10]; Royal Perth Hospital Medical Research Foundation FX This research was supported by grant (HL117933) from the National Heart, Lung, and Blood Institute to BFA and by contract (53-3K06-5-10) from the US Department of Agriculture to EJS. JRB was supported by a Practitioner Fellowship from the Royal Perth Hospital Medical Research Foundation. NR 38 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0021-9150 EI 1879-1484 J9 ATHEROSCLEROSIS JI Atherosclerosis PD OCT PY 2016 VL 253 BP 7 EP 14 DI 10.1016/j.atherosclerosis.2016.08.014 PG 8 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA EE2GG UT WOS:000389401200003 PM 27573733 ER PT J AU Yubero-Serrano, EM Delgado-Lista, J Alcala-Diaz, JF Garcia-Rios, A Perez-Caballero, AI Blanco-Rojo, R Gomez-Delgado, F Marin, C Tinahones, FJ Caballero, J Ordovas, JM van Ommen, B Perez-Jimenez, F Perez-Martinez, P Lopez-Miranda, J AF Yubero-Serrano, Elena M. Delgado-Lista, Javier Alcala-Diaz, Juan F. Garcia-Rios, Antonio Perez-Caballero, Ana I. Blanco-Rojo, Ruth Gomez-Delgado, Francisco Marin, Carmen Tinahones, Francisco J. Caballero, Javier Ordovas, Jose M. van Ommen, Ben Perez-Jimenez, Francisco Perez-Martinez, Pablo Lopez-Miranda, Jose TI A dysregulation of glucose metabolism control is associated with carotid atherosclerosis in patients with coronary heart disease (CORDIOPREV-DIAB study) SO ATHEROSCLEROSIS LA English DT Article DE Coronary heart disease; Intima media thickness; Glycemic control; OGTT; Posprandial glycemia; Age ID INTIMA-MEDIA THICKNESS; PRIOR MYOCARDIAL-INFARCTION; FASTING PLASMA-GLUCOSE; CARDIOVASCULAR-DISEASE; INSULIN-RESISTANCE; ARTERY INTIMA; SUBCLINICAL ATHEROSCLEROSIS; GLYCEMIC CONTROL; RISK-FACTORS; LIFE-STYLE AB Background and aims: Patients with coexisting coronary heart disease (CHD) and type 2 diabetes mellitus (T2DM) are at high risk of cardiovascular recurrence, however, it is not well established whether they exhibit an increased intima-media thickness of both common carotid arteries (IMT-CC). Furthermore, whether this relationship is inherent to T2DM or depends on glycemic control has not been tested in large cohorts. Our aim was to determine whether clinical categories and/or analytical markers of glucose metabolism control were associated with IMT-CC in CHD patients. Methods: 1002 patients aged 20-75 years, categorized into normal glucose tolerance (NGT), impaired fasting glucose (IFG), impaired glucose tolerance (IGT) or T2DM, underwent an oral glucose tolerance test (OGTT) and an IMT-CC measurement. Results: IMT-CC was higher in T2DM patients with HbA1c > 6.5% compared to T2DM patients with HbA1c < 6.5% (p - 0.001), patients with IFG or IGT (p < 0.001) and NGT (p < 0.001). When age was considered, IMT-CC was influenced by glucose metabolism control only in e patients with age < 61 years (p < 0.01). In a multiple linear regression analysis, glucose concentration at 120 min, but not other OGTT time-points appeared as a significant independent contributor of IMT-CC (p < 0.001). Moreover, a multiple logistic regression and the area under curve (AUC) of the ROC curve analysis showed a predictive power of glucose at 120 min to detect those CHD patients at the highest risk, defined as IMT-CC >= 0.7 mm (R-2 = 0.221; AUC = 0.761). Conclusions: Our results highlight the importance of properly controlling glucose metabolism in CHD patients, in younger populations in particular, providing an easy way of categorizing patients with an increased IMT-CC. Moreover, glucose concentration at 120 min could contribute to CVD risk and its determination could be used as a predictive tool to identify those CHD patients at the highest risk. (C) 2016 Elsevier Ireland Ltd. All rights reserved. C1 [Yubero-Serrano, Elena M.; Delgado-Lista, Javier; Alcala-Diaz, Juan F.; Garcia-Rios, Antonio; Perez-Caballero, Ana I.; Blanco-Rojo, Ruth; Gomez-Delgado, Francisco; Marin, Carmen; Tinahones, Francisco J.; Perez-Jimenez, Francisco; Perez-Martinez, Pablo; Lopez-Miranda, Jose] Univ Cordoba, Reina Sofia Univ Hosp, Maimonides Biomed Res Inst Cordoba IMIBIC, Lipids & Atherosclerosis Unit, E-14071 Cordoba, Spain. [Yubero-Serrano, Elena M.; Delgado-Lista, Javier; Alcala-Diaz, Juan F.; Garcia-Rios, Antonio; Perez-Caballero, Ana I.; Blanco-Rojo, Ruth; Gomez-Delgado, Francisco; Marin, Carmen; Perez-Jimenez, Francisco; Perez-Martinez, Pablo; Lopez-Miranda, Jose] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nutr CIBEROBN, Madrid, Spain. [Tinahones, Francisco J.] Univ Malaga, Virgen de la Victoria Hosp, Biomed Res Inst Malaga IBIMA, Malaga, Spain. [Caballero, Javier] Reina Sofia Univ Hosp, Clin Anal Serv, Cordoba, Spain. [Ordovas, Jose M.] Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA. [van Ommen, Ben] TNO, Zeist, Netherlands. RP Delgado-Lista, J; Lopez-Miranda, J (reprint author), Reina Sofia Univ Hosp, IMIBIC, Lipids & Atherosclerosis Res Unit, Avda Menendez Pidal S-N, Cordoba 14004, Spain. EM delgadolista@gmail.com; jlopezmir@uco.es RI Yubero-Serrano, Elena /H-4832-2013; OI Blanco-Rojo, Ruth/0000-0002-6159-3597; Gomez Delgado, Francisco/0000-0002-0216-2084 FU Fundacion Patrimonio Comunal Olivarero; Junta de Andalucia (Consejeria de Salud, Consejeria de Agricultura y Pesca, Consejeria de Innovacion, Ciencia y Empresa); Diputaciones de Jaen y Cordoba; Centro de Excelencia en Investigacion sobre Aceite de Oliva y Salud; Ministerio de Medio Ambiente, MedioRural y Marino, Gobierno de Espana; Consejeria de Salud, Junta de Andalucia [PI0193/09, PI-0252/09, PI-0058/10]; Proyecto de Excelencia, Consejeria de Economia, Innovacion, Ciencia y Empleo [CVI-7450]; Merck Serono and Fundacion; Ministerio de Ciencia e Innovacion [AGL2009-122270]; Ministerio de Economia y Competitividad [AGL2012/39615, FIS PI10/01041, FIS PI13/00023]; Instituto de Salud Carlos III (ISCIII) of Spain; Directorate General for Assessment and Promotion of Research; EU's European Regional Development Fund (FEDER); European Community [289511]; ISCIII (Programa Rio-Hortega) FX The CordioPrev study is supported by the Fundacion Patrimonio Comunal Olivarero, by Junta de Andalucia (Consejeria de Salud, Consejeria de Agricultura y Pesca, Consejeria de Innovacion, Ciencia y Empresa), Diputaciones de Jaen y Cordoba, Centro de Excelencia en Investigacion sobre Aceite de Oliva y Salud and Ministerio de Medio Ambiente, MedioRural y Marino, Gobierno de Espana. The CordioPrev study also received research grants from Consejeria de Salud, Junta de Andalucia (PI0193/09 to J L-M, PI-0252/09 to J D-L, and PI-0058/10 to P P-M); Proyecto de Excelencia, Consejeria de Economia, Innovacion, Ciencia y Empleo (CVI-7450 to J L-M); Merck Serono and Fundacion 2000 (Clinical research in Cardiometabolic to P P-M), and from the Ministerio de Ciencia e Innovacion (AGL2009-122270 to J L-M); Ministerio de Economia y Competitividad (AGL2012/39615 to J L-M, FIS PI10/01041 to P P-M, FIS PI13/00023 to J D-L), integrated into the framework of the National Plan for Scientific Research, Technological Development and Innovation 2008-2011 and 2013-2016, co-financed by the Instituto de Salud Carlos III (ISCIII) of Spain and also by the Directorate General for Assessment and Promotion of Research and the EU's European Regional Development Fund (FEDER). It was also partly supported by a Research Grant from the European Community (NUTRITECH European Integrated Project-289511). F.G.D. is supported by a research contract of ISCIII (Programa Rio-Hortega). The CIBEROBN is an initiative of the ISCIII. The contents of this paper are not intended for any commercial use. NR 55 TC 1 Z9 1 U1 2 U2 2 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0021-9150 EI 1879-1484 J9 ATHEROSCLEROSIS JI Atherosclerosis PD OCT PY 2016 VL 253 BP 178 EP 185 DI 10.1016/j.atherosclerosis.2016.07.903 PG 8 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA EE2GG UT WOS:000389401200027 PM 27451413 ER PT J AU El-Sabaawi, RW Frauendorf, TC Marques, PS Mackenzie, RA Manna, LR Mazzoni, R Phillip, DAT Warbanski, ML Zandona, E AF El-Sabaawi, Rana W. Frauendorf, Therese C. Marques, Piata S. Mackenzie, Richard A. Manna, Luisa R. Mazzoni, Rosana Phillip, Dawn A. T. Warbanski, Misha L. Zandona, Eugenia TI Biodiversity and ecosystem risks arising from using guppies to control mosquitoes SO BIOLOGY LETTERS LA English DT Article DE dengue; ecosystem service; invasion; malaria; Zika ID LIFE-HISTORY EVOLUTION; POECILIA-RETICULATA; TRINIDADIAN GUPPIES; RIVULUS-HARTII; LARVAE; WATER; ADAPTATION; STREAMS; SPREAD; FISH AB Deploying mosquito predators such as the guppy (Poecilia reticulata) into bodies of water where mosquitoes breed is a common strategy for limiting the spread of disease-carrying mosquitoes. Here, we draw on studies from epidemiology, conservation, ecology and evolution to show that the evidence for the effectiveness of guppies in controlling mosquitoes is weak, that the chances of accidental guppy introduction into local ecosystems are large, and that guppies can easily establish populations and damage these aquatic ecosystems. We highlight several knowledge and implementation gaps, and urge that this approach is either abandoned in favour of more effective strategies or that it is used much more rigorously. Controlling mosquitoes does not need to come at the expense of freshwater biodiversity. C1 [El-Sabaawi, Rana W.; Frauendorf, Therese C.; Marques, Piata S.; Warbanski, Misha L.] Univ Victoria, Dept Biol, Stn CSC, POB 1700, Victoria, BC V8W 2Y2, Canada. [Mackenzie, Richard A.] USDA Forest Serv, Inst Pacific Isl, Pacific Southwest Res Stn, Hilo, HI 96720 USA. [Manna, Luisa R.; Mazzoni, Rosana; Zandona, Eugenia] Univ Estado Rio de Janeiro, Dept Ecol, Rua Sao Francisco Xavier 524, BR-20550900 Rio De Janeiro, Brazil. [Phillip, Dawn A. T.] Univ West Indies, Dept Life Sci, St Augustine, Trinid & Tobago. RP El-Sabaawi, RW (reprint author), Univ Victoria, Dept Biol, Stn CSC, POB 1700, Victoria, BC V8W 2Y2, Canada. EM rana@uvic.ca FU IDRC/AUCC; CNPq; Science without Borders (CAPES) fellowship; University of Victoria FX We have received no funding for this study. Past and ongoing work on guppies in our groups has been funded by a number of sources (IDRC/AUCC, CNPq, etc) that are acknowledged in the primary papers we cite in this article. PSM is supported by a Science without Borders (CAPES) fellowship. TCF is supported by funding from the University of Victoria. NR 44 TC 0 Z9 0 U1 14 U2 14 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 1744-9561 EI 1744-957X J9 BIOL LETTERS JI Biol. Lett. PD OCT 1 PY 2016 VL 12 IS 10 AR 20160590 DI 10.1098/rsbl.2016.0590 PG 4 WC Biology; Ecology; Evolutionary Biology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Evolutionary Biology GA EE5HM UT WOS:000389636800011 ER PT J AU Gill, TA Li, J Saenger, M Scofield, SR AF Gill, T. A. Li, J. Saenger, M. Scofield, S. R. TI Thymol-based submicron emulsions exhibit antifungal activity against Fusarium graminearum and inhibit Fusarium head blight in wheat SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE antifungal; biocide; Fusarium graminearum; thymol; wheat ID ESSENTIAL OILS; ANTIMICROBIAL EFFICACY; DEOXYNIVALENOL ACCUMULATION; MYCOTOXIN PRODUCTION; MEMBRANE INTEGRITY; PHENOLIC-COMPOUNDS; F-GRAMINEARUM; FUNGI; NANOEMULSIONS; RESISTANCE AB AimsFusarium graminearum is a very destructive fungal pathogen that leads to Fusarium head blight (FHB) in wheat, a disease which costs growers millions of dollars annually both in crop losses and in remediation efforts. Current countermeasures include the deployment of wheat varieties with some resistance to FHB in conjunction with timed fungicide treatments. In this article, we introduce a fungicide based on thymol, a naturally occurring plant phenolic derived from essential oils. To overcome the hydrophobicity of thymol, the thymol active was incorporated into a low-surfactant submicron emulsion with and without a carrier oil. Methods and ResultsThe minimum fungicidal concentration of F.graminearum was found to be both 002% for thymol emulsions with and without an oil component. Time-to-kill experiments showed that thymol emulsions were able to inactivate F.graminearum in as little as 10s at concentrations above 006%. Spraying the thymol emulsions (similar to 01% range) on the wheat variety Bobwhite demonstrated significant reductions in FHB infection rate (number of infected spikelets). However, with 05% thymol, the wheat heads exhibited premature senescence. Transmission and scanning electron micrographs suggest that the mechanism of antifungal action is membrane mediated, as conidia exposed to thymol showed complete organelle disorganization and evidence of lipid emulsification. ConclusionThe collective experimental data suggest that thymol emulsions may be an effective naturally derived alternative to the current thymol treatments, and chemical fungicides in ameliorating FHB. Significance and Impact of the StudyThis is the first thymol-derived nanoemulsion particles resuspended into water and not DMSO, exhibiting the same antibacterial/antifungal activity as previously described thymol and thyme oil treatments. This drastically reduces the environmental footprint thymol will leave if utilized as a fungicide treatment on field crops. C1 [Gill, T. A.; Scofield, S. R.] Purdue Univ, USDA ARS, Coll Agr, Dept Agron, W Lafayette, IN 47907 USA. [Li, J.; Saenger, M.] Purdue Univ, Coll Vet Med, Dept Basic Med Sci, W Lafayette, IN 47907 USA. RP Gill, TA (reprint author), Univ Florida, Dept Entomol, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. EM tagill@ufl.edu NR 47 TC 0 Z9 0 U1 7 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 EI 1365-2672 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD OCT PY 2016 VL 121 IS 4 BP 1103 EP 1116 DI 10.1111/jam.13195 PG 14 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DW3XG UT WOS:000383575800019 PM 27253757 ER PT J AU Spurgeon, DW Brent, CS AF Spurgeon, D. W. Brent, C. S. TI Development, Survival, and Hatching Periodicity of Lygus hesperus (Hemiptera: Miridae) Eggs under Constant and Variable Temperatures SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE Lygus hesperus; western tarnished plant bug; thermoperiod; development ID WATER-STRESS; CANOPY TEMPERATURE; COTTON; INDICATOR; NYMPHS; IRRIGATION; INSECTS; BUG AB The microclimate within western cotton (Gossypium spp.) seems favorable for the western tarnished plant bug (Lygus hesperus Knight) (Hemiptera: Miridae), provided adequate soil moisture is available. Diminishing water supplies and increasing costs in the West will likely change irrigation practices and induce at least periodically unfavorable conditions for L. hesperus. Knowledge of L. hesperus thermal ecology has been limited to constant temperatures for which relevance to variable temperature environments is unknown. Eggs of L. hesperus were reared under low (mean = 15 degrees C), medium (mean = 22 degrees C), or high (mean = 29 degrees C) constant (+/-<0.5 degrees C) or diurnally fluctuating (+/- 8 degrees C) temperatures. Developmental time and survival were similar between constant and variable regimes at the medium temperature. In contrast, variable low temperatures hastened egg development and increased survival, whereas variable high temperatures delayed development and reduced survival compared with constant regimes. Within the studied temperature range, the relationship between temperature and egg developmental rate was linear for constant temperatures, but a quadratic term was needed to describe this relationship under variable temperatures. Under medium variable temperatures, egg hatch was disproportionately high during the warmest period of the day (1300-1900 h) compared with the constant regime. Differences between regimes were less pronounced at high temperatures, except for the conspicuous absence of hatch between 1300 and 1900 h in the variable regime when temperatures were always >32 degrees C. These results indicate the limited utility of constant temperature data for understanding L. hesperus thermal ecology and provide baseline information to better plan and interpret applied studies of L. hesperus thermal ecology. C1 [Spurgeon, D. W.; Brent, C. S.] ARS, USDA, Pest Management & Biocontrol Res Unit, 21881 N Cardon Lane, Maricopa, AZ 85138 USA. RP Spurgeon, DW (reprint author), ARS, USDA, Pest Management & Biocontrol Res Unit, 21881 N Cardon Lane, Maricopa, AZ 85138 USA. EM dale.spurgeon@ars.usda.gov NR 35 TC 0 Z9 0 U1 5 U2 5 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD OCT PY 2016 VL 51 IS 4 BP 292 EP 304 PG 13 WC Entomology SC Entomology GA EE4GT UT WOS:000389561000003 ER PT J AU Suh, CPC Spurgeon, DW AF Suh, C. P. -C. Spurgeon, D. W. TI Continued Pheromone Release by Boll Weevils (Coleoptera: Curculionidae) Following Host Removal SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE boll weevil; pheromone; traps; cotton; food removal C1 [Suh, C. P. -C.; Spurgeon, D. W.] USDA ARS, Insect Control & Cotton Dis Res Unit, 2771 F&B Rd, College Stn, TX 77845 USA. [Spurgeon, D. W.] USDA ARS, Pest Management & Biocontrol Res Unit, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. RP Suh, CPC (reprint author), USDA ARS, Insect Control & Cotton Dis Res Unit, 2771 F&B Rd, College Stn, TX 77845 USA. EM charles.suh@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD OCT PY 2016 VL 51 IS 4 BP 332 EP 335 PG 4 WC Entomology SC Entomology GA EE4GT UT WOS:000389561000009 ER PT J AU Petersen, MK Muscha, JM Mulliniks, JT Roberts, AJ AF Petersen, M. K. Muscha, J. M. Mulliniks, J. T. Roberts, A. J. TI Water temperature impacts water consumption by range cattle in winter SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE in vitro; neutral detergent fiber disappearance; range cows; rumen temperature; water intake; water temperature ID FEED-INTAKE; COWS; RUMEN; SHEEP; SNOW AB Water consumption and DMI have been found to be positively correlated, and both may interact with ingestion of cold water or grazed frozen forage due to transitory reductions in the temperature of ruminal contents. The hypothesis underpinning the study explores the potential that cows provided warm drinking water would have increased in situ NDF and OM disappearances and a more stable rumen temperature, drink more water, and lose less BW during the winter. This hypothesis was tested in 3 experiments. In Exp. 1, ruminal extrusa (93.1% DM, 90.2% OM, 81.1% NDF [DM], and 4.9% CP [DM]) were randomly allocated to 1 of 5 in vitro incubation temperatures. In 2 independent trials, temperatures evaluated were 39, 37, or 35 degrees C (trial 1) and 39, 33, or 31 degrees C (trial 2). In Exp. 2, 4 pregnant rumen cannulated cows grazing in January were fitted with Kahne (KB1000) temperature continuous recording boluses for 22 d. Two grazed in a paddock provided cold water (8.2 degrees C) and 2 in a paddock provided warm water (31.1 degrees C). Two in situ trials were conducted placing 6 in situ bags containing 2 g of winter range ruminal extrusa in each of the 4 ruminally cannulated cows and incubating bags for 72 h for measurement of NDF disappearance. In Exp. 3, 6 paddocks (n = 3/water treatment) were grazed by 10 to 13 pregnant crossbred Angus cows from December through February across 3 yr from 2009 to 2012. Water intake per paddock was measured daily and ambient temperature was recorded. Motion-activated cameras were used to determine the time of day water was consumed and the number of cow appearances at water. In Exp. 1, rate and total gas production (P < 0.05) and NDF disappearance (P < 0.001) at 48 h was reduced by each incubation temperature below 39 degrees C. In Exp. 2, ruminal temperature for cows supplied with warm water dropped below 38 degrees C 1.5% of the time whereas ruminal temperature for cows provided cold water dropped below 38 degrees C 9.4% of the time (P < 0.01). Drinking water temperature did not alter in situ OM or NDF disappearance. In Exp. 3, cows with access to warm water consumed 30% (P < 0.05) more water than cows provided cold water. In this study, there were energetic costs to range cows proportional to consumption of water at temperatures less than body temperature. The magnitude of these costs were found to be less than the heat increment because no improvement to BW gain, BCS change, or calf birth weight were found for cows consuming warmed water. C1 [Petersen, M. K.; Muscha, J. M.; Roberts, A. J.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. [Mulliniks, J. T.] Univ Tennessee, Dept Anim Sci, Crossville, TN 38571 USA. RP Petersen, MK (reprint author), USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. EM Mark.Petersen@ars.usda.gov NR 23 TC 0 Z9 0 U1 3 U2 3 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD OCT PY 2016 VL 94 IS 10 BP 4297 EP 4306 DI 10.2527/jas2015-0155 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA ED6GZ UT WOS:000388955400025 PM 27898880 ER PT J AU Walter, LJ Cole, NA Jennings, JS Hutcheson, JP Meyer, BE Schmitz, AN Reed, DD Lawrence, TE AF Walter, L. J. Cole, N. A. Jennings, J. S. Hutcheson, J. P. Meyer, B. E. Schmitz, A. N. Reed, D. D. Lawrence, T. E. TI The effect of zilpaterol hydrochloride supplementation on energy metabolism and nitrogen and carbon retention of steers fed at maintenance and fasting intake levels SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE beef; energy metabolism; nutrient digestibility; zilpaterol hydrochloride ID BETA-ADRENERGIC AGONISTS; WET DISTILLERS GRAINS; CORN-BASED DIETS; CARCASS CHARACTERISTICS; METHANE EMISSIONS; FEEDLOT CATTLE; PROTEIN-TURNOVER; FINISHING DIET; MEAT QUALITY; BEEF STEERS AB An indirect calorimetry trial examined energy metabolism, apparent nutrient digestibility, C retention (CR), and N retention (NR) of cattle supplemented with zilpaterol hydrochloride (ZH). Beef steers (n = 20; 463 +/- 14 kg) blocked (n = 5) by weight and source were individually fed and adapted to maintenance energy intake for 21 d before allotment to ZH (90 mg/steerd) or no beta(2)-adrenergic agonist treatment (control [CONT]) for 20 d (455 +/- 14 kg at the start of treatment). Respiration chambers = 4 were used to quantify heat production (HP) during maintenance (d 12 to 16 of the ZH period) and fasting heat production (FHP; d 19 to 20 of ZH period; total 4 d of fast). Steers were harvested after a 6-d ZH withdrawal and carcasses were graded 24 h after harvest. Control cattle lost more BW (P < 0.01; 9 kg for CONT and 2 kg for ZH-treated) during maintenance whereas the BW loss of ZH-treated steers was greater (P < 0.01; 9 kg for ZH-treated and vs. 4 kg, for CONT) during FHP; no differences (P >= 0.76) were detected for G: F, ADG, and end BW. No differences in DMI, apparent nutrient digestibility, O-2 consumption, or CH4 production (P >= 0.12) were detected; however, ZH-treated cattle had greater CO2 production during maintenance (P = 0.04; 23.6 L/kgBW for ZH-treated and 22.4 L/kg BW0.75 for CONT). Digestible energy and ME did not differ (P >= 0.19); however, urinary energy was greater (P = 0.05; 0.091 Mcal for CONT and 0.074 Mcal for ZH-treated) in CONT cattle. Steers treated with ZH tended to have greater HP (P = 0.09; 12.44 Mcal for ZH-treated and 11.69 Mcal for CONT), but the effect was reduced on a BW0.75 basis (P = 0.12; 0.126 Mcal/kg BW0.75 for ZH-treated and 0.120 Mcal/kg BW0.75 for CONT vs. 0.120 Mcal/kg BW0.75). No treatment difference in FHP was observed (P >= 0.32) although CO2 production (L/steer) increased with ZH treatment (P = 0.04; 1,423 L/steer for ZH-treated and 1,338 L/steer for CONT). Control cattle excreted more (P = 0.05) N in urine (39.8 g/d for CONT and 32.4 g/d for ZH-treated); therefore, NR (P = 0.07; 22.14 g/d for ZH-treated and 14.12 g/d for CONT steers) tended to be greater for ZH-fed steers. Steers treated with ZH lost more C via CO2 (P = 0.04; 1,036.9 g/d for ZH-treated and 974.3 g/d for CONT) although total CR did not differ (P >= 0.23). Empty BW, HCW, and harvest yields (g/kg empty BW) were not different (P >= 0.13), whereas ZH increased dressed yield (P = 0.02; 62.12 % for ZH-treated and 60.65% for CONT) and LM area (P = 0.02; 77.81 cm(2) for ZH-treated and vs. 70.90 cm(2) for CONT). Separable carcass lean and actual skeletal muscle protein (SMP) were increased with ZH (P <= 0.04; 201.6 and 41.2 kg, respectively for ZH-treated and 196.0 and 38.4 kg, respectively for CONT). Results from this trial indicate that ZH treatment increased (P = 0.03) SMP and tended (P >= 0.07) to increase NR and modify HP during maintenance by increasing CO2 production. C1 [Walter, L. J.; Schmitz, A. N.; Reed, D. D.; Lawrence, T. E.] West Texas A&M Univ, Beef Carcass Res Ctr, Dept Agr Sci, Canyon, TX 79016 USA. [Cole, N. A.; Meyer, B. E.] USDA ARS, CPRL, Bushland, TX 79012 USA. [Jennings, J. S.] Texas A&M AgriLife Res, Amarillo, TX 79106 USA. [Hutcheson, J. P.] Merck Anim Hlth, Summit, NJ 07901 USA. RP Lawrence, TE (reprint author), West Texas A&M Univ, Beef Carcass Res Ctr, Dept Agr Sci, Canyon, TX 79016 USA. EM tlawrence@wtamu.edu FU Merck Animal Health FX Financial support for this project was provided by Merck Animal Health. J. P. Hutcheson is employed by Merck Animal Health. NR 40 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD OCT PY 2016 VL 94 IS 10 BP 4401 EP 4414 DI 10.2527/jas2016-0612 PG 14 WC Agriculture, Dairy & Animal Science SC Agriculture GA ED6GZ UT WOS:000388955400035 PM 27898856 ER PT J AU Overholt, MF Arkfeld, EK Mohrhauser, DA King, DA Wheeler, TL Dilger, AC Shackelford, SD Boler, DD AF Overholt, M. F. Arkfeld, E. K. Mohrhauser, D. A. King, D. A. Wheeler, T. L. Dilger, A. C. Shackelford, S. D. Boler, D. D. TI Comparison of variability in pork carcass composition and quality between barrows and gilts SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE barrow; composition; gilt; quality; variability ID DISTILLERS DRIED GRAINS; IMMUNOLOGICAL CASTRATION IMPROVEST; FINISHING PIGS; SENSORY CHARACTERISTICS; GROWTH-PERFORMANCE; FAT QUALITY; RACTOPAMINE HYDROCHLORIDE; BELLY CHARACTERISTICS; MEAT QUALITY; SEX AB Pigs (n = 8,042) raised in 8 different barns representing 2 seasons (cold and hot) and 2 production focuses (lean growth and meat quality) were used to characterize variability of carcass composition and quality traits between barrows and gilts. Data were collected on 7,684 pigs at the abattoir. Carcass characteristics, subjective loin quality, and fresh ham face color (muscles) were measured on a targeted 100% of carcasses. Fresh belly characteristics, boneless loin weight, instrumental loin color, and ultimate loin pH measurements were collected from 50% of the carcasses each slaughter day. Adipose tissue iodine value (IV), 30-min loin pH, LM slice shear force, and fresh ham muscle characteristic measurements were recorded on 10% of carcasses each slaughter day. Data were analyzed using the MIXED procedure of SAS as a 1-way ANOVA in a randomized complete block design with 2 levels (barrows and gilts). Barn (block), marketing group, production focus, and season were random variables. A 2-variance model was fit using the REPEATED statement of the MIXED procedure, grouped by sex for analysis of least squares means. Homogeneity of variance was tested on raw data using Levene's test of the GLM procedure. Hot carcass weight of pigs (94.6 kg) in this study was similar to U. S. industry average HCW (93.1 kg). Therefore, these data are representative of typical U. S. pork carcasses. There was no difference (P >= 0.09) in variability of HCW or loin depth between barrow and gilt carcasses. Back fat depth and estimated carcass lean were more variable (P <= 0.0001) and IV was less variable (P = 0.05) in carcasses from barrows than in carcasses from gilts. Fresh belly weight and thickness were more variable (P <= 0.01) for bellies of barrows than bellies of gilts, but there was no difference in variability for belly length, width, or flop distance (P >= 0.06). Fresh loin subjective color was less variable (P < 0.01) and subjective marbling was more variable (P < 0.0001) in loins from barrows than in those from gilts, but there were no differences (P >= 0.08) in variability for any other loin traits or fresh ham traits. Overall, traits associated with carcass fatness, including back fat depth, belly thickness, and marbling, but not IV, were more variable in carcasses from barrows than in carcasses from gilts, whereas minimal differences in variability existed between carcasses of barrows and carcasses of gilts for traits associated with carcass muscling and lean quality. C1 [Overholt, M. F.; Arkfeld, E. K.; Dilger, A. C.; Boler, D. D.] Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA. [Mohrhauser, D. A.] Smithfield Foods, Denison, IA 51442 USA. [King, D. A.; Wheeler, T. L.; Shackelford, S. D.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Boler, DD (reprint author), Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA. EM dboler2@illinois.edu FU National Pork Board FX Funding, wholly or in part, was provided by The National Pork Board. NR 35 TC 1 Z9 1 U1 8 U2 8 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD OCT PY 2016 VL 94 IS 10 BP 4415 EP 4426 DI 10.2527/jas2016-0702 PG 12 WC Agriculture, Dairy & Animal Science SC Agriculture GA ED6GZ UT WOS:000388955400036 PM 27898864 ER PT J AU Fombong, AT Mutunga, JM Teal, PEA Torto, B AF Fombong, Ayuka T. Mutunga, Jacqueline M. Teal, Peter E. A. Torto, Baldwyn TI Behavioral Evidence for Olfactory-Based Location of Honeybee Colonies by the Scarab Oplostomus haroldi SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Coleoptera; Scarabaeidae; Large hive beetle; Esters; Honeybee; Kairomone; Cetoniid ID BEETLE AETHINA-TUMIDA; APIS-MELLIFERA; COLEOPTERA SCARABAEIDAE; LABORATORY CONDITIONS; PACHNODA-INTERRUPTA; RECEPTOR NEURONS; VOLATILES; NITIDULIDAE; DIVERSITY; PHEROMONE AB The Afro-tropical scarab Oplostomus haroldi (Witte) is a pest of honeybees in East Africa with little information available on its chemical ecology. Recently, we identified a female-produced contact sex pheromone, (Z)-9-pentacosene, from the cuticular lipids that attracted males. Here, we investigated the kairomonal basis of host location in O. haroldi. We used coupled gas chromatography/electroantennographic detection (GC/EAD) and GC/mass spectrometry to identify antennally-active compounds from volatiles collected from honeybee colonies. Antennae of both sexes of the beetle consistently detected seven components, which were identified as 3-hydroxy-2-butanone, 2,3-butanediol, butyl acetate, isopentyl acetate, butyl butyrate, hexyl acetate, and methyl benzoate. In olfactometer bioassays, both sexes responded to the full seven-component synthetic blend over solvent controls, but chose honeybee colony odors over the blend. These findings suggest that the seven compounds are components of a kairomone from honeybee colonies used by O. haroldi. C1 [Fombong, Ayuka T.; Mutunga, Jacqueline M.; Torto, Baldwyn] Int Ctr Insect Physiol & Ecol Icipe, POB 30772-00100, Nairobi, Kenya. [Teal, Peter E. A.] USDA ARS, CMAVE, 1600-1700 SW23rd Dr, Gainesville, FL 32608 USA. RP Torto, B (reprint author), Int Ctr Insect Physiol & Ecol Icipe, POB 30772-00100, Nairobi, Kenya. EM btorto@icipe.org FU German Academic Exchange Service (DAAD) from United States Department of Agriculture - Agricultural Research Service (USDA-ARS) [SCA-586615-7-119F] FX The authors are grateful to Dr. D. Salifu for statistical advice; R. Herisolo for the olfactometer drawing; J. Kilonzo, J. Ngang'a, and A Mwajeve for field assistance with bee odors, frame, and beetle collections from the apiary. The authors are grateful to two anonymous reviewers for comments on an earlier version of the manuscript. The German Academic Exchange Service (DAAD) funded ATF while project funding came from the United States Department of Agriculture - Agricultural Research Service (USDA-ARS Project No. SCA-586615-7-119F). NR 33 TC 0 Z9 0 U1 5 U2 5 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 EI 1573-1561 J9 J CHEM ECOL JI J. Chem. Ecol. PD OCT PY 2016 VL 42 IS 10 BP 1063 EP 1069 DI 10.1007/s10886-016-0748-1 PG 7 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA ED4HB UT WOS:000388807500009 PM 27534749 ER PT J AU Goodrich, BA Waring, KM Kolb, TE AF Goodrich, Betsy A. Waring, Kristen M. Kolb, Thomas E. TI Genetic variation in Pinus strobiformis growth and drought tolerance from southwestern US populations SO TREE PHYSIOLOGY LA English DT Article DE adaptive traits; carbon isotope ratio; five-needle pine; southwestern white pine ID CLIMATE-CHANGE; BLISTER RUST; DOUGLAS-FIR; TREE POPULATIONS; NORTH-AMERICA; GAS-EXCHANGE; FOREST; RESPONSES; PONDEROSA; SEEDLINGS AB The persistence of some tree species is threatened by combinations of novel abiotic and biotic stressors. To examine the hypothesis that Pinus strobiformis Engelm., a tree threatened by an invasive forest pathogen and a changing climate, exhibits intraspecific genetic variation in adaptive traits, we conducted a common garden study of seedlings at one location with two watering regimes using 24 populations. Four key findings emerged: (i) growth and physiological traits were low to moderately differentiated among populations but differentiation was high for some traits in water-stressed populations; (ii) seedlings from warmer climates grew larger, had higher stomatal density and were more water-use efficient (as measured by the carbon isotope ratio) than populations from colder climates; (iii) seedlings from the northern edge of the species' distribution had lower water-use efficiency, higher stomatal conductance, slower growth and longer survival in a lethal drought experiment compared with seedlings from more southern populations; and (iv) based on non-metric multidimensional scaling analyses, populations clustered into southern and northern groups, which did not correspond to current seed transfer zones. Our discovery of a clinal geographic pattern of genetic variation in adaptive traits of P. strobiformis seedlings will be useful in developing strategies to maintain the species during ongoing climate change and in the face of an invasive pathogen. C1 [Goodrich, Betsy A.; Waring, Kristen M.; Kolb, Thomas E.] No Arizona Univ, Sch Forestry, Flagstaff, AZ 86011 USA. [Goodrich, Betsy A.] US Forest Serv, Forest Hlth Protect, USDA, Wenatchee Forestry Sci Lab, 1133 N Western Ave, Wenatchee, WA 98801 USA. RP Goodrich, BA (reprint author), No Arizona Univ, Sch Forestry, Flagstaff, AZ 86011 USA.; Goodrich, BA (reprint author), US Forest Serv, Forest Hlth Protect, USDA, Wenatchee Forestry Sci Lab, 1133 N Western Ave, Wenatchee, WA 98801 USA. EM agoodrich@fs.fed.us FU McIntire-Stennis; State of Arizona, a Genes to Environment Fellowship through NAU Department of Biology; USDA USFS Forest Health Protection FX Funding for this project was provided by McIntire-Stennis appropriations to NAU and the State of Arizona, a Genes to Environment Fellowship through NAU Department of Biology, and the USDA USFS Forest Health Protection. NR 62 TC 0 Z9 0 U1 8 U2 8 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0829-318X EI 1758-4469 J9 TREE PHYSIOL JI Tree Physiol. PD OCT PY 2016 VL 36 IS 10 BP 1219 EP 1235 DI 10.1093/treephys/tpw052 PG 17 WC Forestry SC Forestry GA ED6CK UT WOS:000388942100004 PM 27344065 ER PT J AU Royo, AA Kramer, DW Miller, KV Nibbelink, NP Stout, SL AF Royo, Alejandro A. Kramer, David W. Miller, Karl V. Nibbelink, Nathan P. Stout, Susan L. TI The canary in the coal mine: Sprouts as a rapid indicator of browse impact in managed forests SO ECOLOGICAL INDICATORS LA English DT Article DE Odocoileus virginianus; Deer; Herbivory; Stump sprout; Disturbance ID WHITE-TAILED DEER; EASTERN DECIDUOUS FOREST; PELLET-GROUP COUNTS; ECOLOGICAL IMPACTS; HERBIVORE DENSITY; SHADE TOLERANCE; TREE GROWTH; VEGETATION; REGENERATION; DISTURBANCE AB Forest managers are frequently confronted with sustaining vegetation diversity and structure in landscapes experiencing high ungulate browsing pressure. Often, managers monitor browse damage and risk to plant communities using vegetation as indicators (i.e., phytoindicators). Although useful, the efficacy of traditional phytoindicators is sometimes hampered by limited distribution and abundance, variable browse susceptibility, and lagged responses. In contrast, sprouts possess traits which make them readily available and attractive to browsers, yet fairly resilient to tissue loss. Here, we experimentally evaluate whether hardwood tree stump sprouts are effective and sensitive phytoindicators of deer browse pressure. We measured sprout abundance and height in fenced and unfenced plots at 17 shelterwood harvested sites scattered across a 6500 km(2) region where deer densities varied by nearly an order of magnitude. We found browsing did not alter the proportion of stumps sprouting and sprout density; however, browse pressure varied among the four most abundant species. Acer rubrum and Acer saccharum were heavily browsed, although browse pressure on A. saccharum decreased in areas with greater canopy openness. Fagus grandifolia and Prunus serotina were less preferred. Differences in palatability altered size hierarchies. Averaged across all species, browsing reduced sprout height by 39%, relative to protected sprouts. Under ambient browsing, P. serotina was 60-100% taller than other species and significantly taller than A. saccharum and F. grandifolia. However, within fences A. saccharum and A. rubrum doubled in size, relative to browsed individuals, and were as tall as P. serotina. Deer impact on sprout height within unfenced forest stands was negatively correlated with estimated deer densities (R-2=0.46). Thus, we suggest sprout surveys can provide a measure of impact across much larger areas. Our results demonstrate that sprouts, particularly those of Acer species, offer an abundant, easily measured, and reliable indicator of browse pressure. Moreover, browse impacts on sprouts emerged before impacts were detected on seedling abundance, height, or biomass. We argue sprouts can warn of imminent browse risk to seedlings (and perhaps non-woody vegetation) and thereby allow managers to take actions to mitigate or avert losses to the regenerating seedling cohort. Published by Elsevier Ltd. C1 [Royo, Alejandro A.; Stout, Susan L.] USDA Forest Serv, Northern Res Stn, POB 267, Irvine, PA 16329 USA. [Kramer, David W.; Miller, Karl V.; Nibbelink, Nathan P.] Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. RP Royo, AA (reprint author), USDA Forest Serv, Northern Res Stn, POB 267, Irvine, PA 16329 USA. EM aroyo@fs.fed.us FU USDA Forest Service Northern Research Station; University of Georgia Warnell School of Forestry and Natural Resources; USDA-AFRI Award [12-IA-11242302-093] FX This work was supported by the USDA Forest Service Northern Research Station, the University of Georgia Warnell School of Forestry and Natural Resources, and USDA-AFRI Award #12-IA-11242302-093. We are indebted to the Allegheny National Forest, the Pennsylvania Bureau of Forestry, Bradford Water Authority, Forest Investment Associates, Generations Forestry, Hancock Forest Management, Landvest, and Kane Hardwoods for field sites. We thank Charles Vandever for assisting with data collection and Todd Ristau, Scott Stoleson, Patricia Raymond, Tara Keyser, and two anonymous reviewers for valuable comments on prior versions of this manuscript. NR 77 TC 0 Z9 0 U1 6 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1470-160X EI 1872-7034 J9 ECOL INDIC JI Ecol. Indic. PD OCT PY 2016 VL 69 BP 269 EP 275 DI 10.1016/j.ecolind.2016.04.030 PG 7 WC Biodiversity Conservation; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA ED3YT UT WOS:000388785100027 ER PT J AU Williamson, JC Bestelmeyer, BT McClaran, MP Robinett, D Briske, DD Wu, XB Fernandez-Gimenez, ME AF Williamson, Jeb C. Bestelmeyer, Brandon T. McClaran, Mitchel P. Robinett, Dan Briske, David D. Wu, X. Ben Fernandez-Gimenez, Maria E. TI Can ecological land classification increase the utility of vegetation monitoring data? SO ECOLOGICAL INDICATORS LA English DT Article DE Biological invasion; Land classification; Monitoring; Soil texture; Time series; Vegetation indicator ID TRANSITION MODELS; DESERT GRASSLAND; SOIL TEXTURE; DYNAMICS; ENCROACHMENT; MANAGEMENT; RANGELANDS; SITES; AUSTRALIA; ECOSYSTEM AB Vegetation dynamics in rangelands and other ecosystems are known to be mediated by topoedaphic properties. Vegetation monitoring programs, however, often do not consider the impact of soils and other sources of landscape heterogeneity on the temporal patterns observed. Ecological sites (ES) comprise a land classification system based on soil, topographic, and climate variations that can be readily applied by land managers to classify topoedaphic properties at monitoring locations. We used a long-term (>40y) vegetation record from southeastern Arizona, USA to test the utility of an ES classification for refining interpretations of monitoring data in an area of relatively subtle soil differences. We focused on two phenomena important to rangeland management in the southeastern Arizona region: expansion of the native tree velvet mesquite (Prosopis velutina Woot.) and spread of the introduced perennial grass Lehmann lovegrass (Eragrostis lehmanniana Nees). Specifically, we sought to determine if a quantitative, ES-specific analysis of the long-term record would (1) improve detection of changes in plant species having heightened ecological or management importance and (2) further clarify topoedaphic effects on vegetation trajectories. We found that ES class membership was a significant factor explaining spatiotemporal variation in velvet mesquite canopy cover, Lehmann lovegrass basal cover, and Lehmann lovegrass density measurements. In addition, we observed that the potential magnitude of velvet mesquite and Lehmann lovegrass increases varied substantially among ES classes. Our study brings attention to a practical land management tool that might be called upon to increase the effectiveness of vegetation-based indicators of ecosystem change. Published by Elsevier Ltd. C1 [Williamson, Jeb C.; Bestelmeyer, Brandon T.] New Mexico State Univ, USDA ARS Jornada Expt Range, MSC 3JER,Box 30003, Las Cruces, NM 88003 USA. [McClaran, Mitchel P.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ USA. [Robinett, Dan] Robinett Rangeland Resources LLC, Elgin, AZ USA. [Briske, David D.; Wu, X. Ben] Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX USA. [Fernandez-Gimenez, Maria E.] Colorado State Univ, Dept Forest & Rangeland Stewardship, Ft Collins, CO 80523 USA. RP Williamson, JC (reprint author), New Mexico State Univ, USDA ARS Jornada Expt Range, MSC 3JER,Box 30003, Las Cruces, NM 88003 USA. EM jcwill@nmsu.edu FU USDA CSREES [2007-38415-18637, 2008-51130-19567] FX This research was supported in part by USDA CSREES grants 2007-38415-18637 and 2008-51130-19567. NR 49 TC 1 Z9 1 U1 5 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1470-160X EI 1872-7034 J9 ECOL INDIC JI Ecol. Indic. PD OCT PY 2016 VL 69 BP 657 EP 666 DI 10.1016/j.ecolind.2016.05.030 PG 10 WC Biodiversity Conservation; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA ED3YT UT WOS:000388785100064 ER PT J AU Clifford, JC Buchanan, A Vining, O Kidarsa, TA Chang, JH McPhail, KL Loper, JE AF Clifford, Jennifer C. Buchanan, Alex Vining, Oliver Kidarsa, Teresa A. Chang, Jeff H. McPhail, Kerry L. Loper, Joyce E. TI Phloroglucinol functions as an intracellular and intercellular chemical messenger influencing gene expression in Pseudomonas protegens SO ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID ANTIFUNGAL METABOLITE PRODUCTION; SIGMA-FACTOR SIGMA(S); PYTHIUM DAMPING-OFF; FLUORESCENS PF-5; BIOLOGICAL-CONTROL; ESCHERICHIA-COLI; PYOLUTEORIN PRODUCTION; ANTIBIOTIC PRODUCTION; CELL COMMUNICATION; COTTON SEEDLINGS AB Bacteria can be both highly communicative and highly competitive in natural habitats and antibiotics are thought to play a role in both of these processes. The soil bacterium Pseudomonas protegens Pf-5 produces a spectrum of antibiotics, two of which, pyoluteorin and 2,4-diacetylphloroglucinol (DAPG), function in intracellular and intercellular communication, both as autoinducers of their own production. Here, we demonstrate that phloroglucinol, an intermediate in DAPG biosynthesis, can serve as an intercellular signal influencing the expression of pyoluteorin biosynthesis genes, the production of pyoluteorin, and inhibition of Pythium ultimum, a phytopathogenic oomycete sensitive to pyoluteorin. Through analysis of RNAseq data sets, we show that phloroglucinol had broad effects on the transcriptome of Pf-5, significantly altering the transcription of more than two hundred genes. The effects of nanomolar versus micromolar concentrations of phloroglucinol differed both quantitatively and qualitatively, influencing the expression of distinct sets of genes or having opposite effects on transcript abundance of certain genes. Therefore, our results support the concept of hormesis, a phenomenon associated with signalling molecules that elicit distinct responses at different concentrations. Phloroglucinol is the first example of an intermediate of antibiotic biosynthesis that functions as a chemical messenger influencing gene expression in P. protegens. C1 [Clifford, Jennifer C.; Kidarsa, Teresa A.; Loper, Joyce E.] ARS, USDA, Hort Crops Res Lab, Corvallis, OR 97330 USA. [Buchanan, Alex; Chang, Jeff H.; Loper, Joyce E.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Buchanan, Alex; Chang, Jeff H.; Loper, Joyce E.] Oregon State Univ, Ctr Genome Res & Biocomp, Corvallis, OR 97331 USA. [Vining, Oliver; McPhail, Kerry L.] Oregon State Univ, Coll Pharm, Corvallis, OR 97331 USA. RP Loper, JE (reprint author), ARS, USDA, Hort Crops Res Lab, Corvallis, OR 97330 USA.; Loper, JE (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA.; Loper, JE (reprint author), Oregon State Univ, Ctr Genome Res & Biocomp, Corvallis, OR 97331 USA. EM joyce.loper@ars.usda.gov OI Loper, Joyce/0000-0003-3501-5969 FU National Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2011-67019-30192] FX We are grateful to Jeff Kimbrel, Allison Creason, Brenda Shaffer, Ashley Bixenstein and Marcella Henkels for their assistance; Jerry Weiland for advice on the assays evaluating inhibition of Pythium ultimum; and Mark Desenko, Matthew Peterson and Chris Sullivan of the Center for Genomics Research and Biocomputing at Oregon State University for sequencing expertise and services. This project was supported by National Research Initiative Competitive Grant 2011-67019-30192 from the USDA National Institute of Food and Agriculture. NR 73 TC 2 Z9 2 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1462-2912 EI 1462-2920 J9 ENVIRON MICROBIOL JI Environ. Microbiol. PD OCT PY 2016 VL 18 IS 10 SI SI BP 3296 EP 3308 DI 10.1111/1462-2920.13043 PG 13 WC Microbiology SC Microbiology GA ED1NW UT WOS:000388613200009 PM 26337778 ER PT J AU Song, CX Kidarsa, TA van de Mortel, JE Loper, JE Raaijmakers, JM AF Song, Chunxu Kidarsa, Teresa A. van de Mortel, Judith E. Loper, Joyce E. Raaijmakers, Jos M. TI Living on the edge: emergence of spontaneous gac mutations in Pseudomonas protegens during swarming motility SO ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID FLUORESCENS PF-5; CELL-DIFFERENTIATION; PROTEUS-MIRABILIS; PATHOGENIC BACTERIA; MYXOCOCCUS-XANTHUS; PHASE VARIATION; AERUGINOSA; MUTANTS; COLONIZATION; RECOGNITION AB Swarming motility is a flagella-driven multicellular behaviour that allows bacteria to colonize new niches and escape competition. Here, we investigated the evolution of specific mutations in the GacS/GacA two-component regulatory system in swarming colonies of Pseudomonas protegens Pf-5. Experimental evolution assays showed that repeated rounds of swarming by wildtype Pf-5 drives the accumulation of gacS/gacA spontaneous mutants on the swarming edge. These mutants cannot swarm on their own because they lack production of the biosurfactant orfamide A, but they do co-swarm with orfamide-producing wildtype Pf-5. These co-swarming assays further demonstrated that DgacA mutant cells indeed predominate on the edge and that initial DgacA: wildtype Pf-5 ratios of at least 2: 1 lead to a collapse of the swarming colony. Subsequent whole-genome transcriptome analyses revealed that genes associated with motility, resource acquisition, chemotaxis and efflux were significantly upregulated in DgacA mutant on swarming medium. Moreover, transmission electron microscopy showed that DgacA mutant cells were longer and more flagellated than wildtype cells, which may explain their predominance on the swarming edge. We postulate that adaptive evolution through point mutations is a common feature of range-expanding microbial populations and that the putative fitness benefits of these mutations during dispersal of bacteria into new territories are frequency-dependent. C1 [Song, Chunxu; Raaijmakers, Jos M.] Netherlands Inst Ecol, Dept Microbial Ecol, Droevendaalsesteeg 10, NL-6708 PB Wageningen, Netherlands. [Song, Chunxu; van de Mortel, Judith E.] Wageningen Univ, Lab Phytopathol, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands. [Kidarsa, Teresa A.; Loper, Joyce E.] ARS, USDA, Corvallis, OR 97330 USA. [Raaijmakers, Jos M.] Leiden Univ, Microbial Biotechnol Dept, Inst Biol IBL, Leiden, Netherlands. [van de Mortel, Judith E.] HAS Univ Appl Sci, NL-5911 KJ Venlo, Netherlands. RP Raaijmakers, JM (reprint author), Netherlands Inst Ecol, Dept Microbial Ecol, Droevendaalsesteeg 10, NL-6708 PB Wageningen, Netherlands.; Raaijmakers, JM (reprint author), Leiden Univ, Microbial Biotechnol Dept, Inst Biol IBL, Leiden, Netherlands. EM j.raaijmakers@nioo.knaw.nl RI Raaijmakers, Jos/D-1574-2014; OI Raaijmakers, Jos/0000-0003-1608-6614; KNAW, NIOO-KNAW/0000-0002-3835-159X; Loper, Joyce/0000-0003-3501-5969 FU Graduate School of Experimental Plant Sciences (EPS); Organisation for Economic Cooperation and Development (OECD) FX We are very grateful to the Graduate School of Experimental Plant Sciences (EPS) and the Organisation for Economic Cooperation and Development (OECD) for partially financing this project. We thank the Dutch Genomics Service & Support Provider for conducting the microarray analysis. We thank Marcel Giesbers (Wageningen Electron Microscopy Centre, The Netherlands) for conducting the transmission electron microscopy, Ester Dekkers (Phytopathology Department, Wageningen University) for genetically characterizing the mutations in the spontaneous mutants, Stephen Heeb and Dieter Haas for providing pME6000, and Dr. Irene de Bruijn, Dr. Daniel Roozen and Dr. Arjan de Visser for critically reading the manuscript and excellent suggestions. This publication is no. 6041 of The Netherlands Institute of Ecology (NIOO-KNAW). NR 46 TC 0 Z9 0 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1462-2912 EI 1462-2920 J9 ENVIRON MICROBIOL JI Environ. Microbiol. PD OCT PY 2016 VL 18 IS 10 SI SI BP 3453 EP 3465 DI 10.1111/1462-2920.13288 PG 13 WC Microbiology SC Microbiology GA ED1NW UT WOS:000388613200019 PM 26945503 ER PT J AU Loper, JE Henkels, MD Rangel, LI Olcott, MH Walker, FL Bond, KL Kidarsa, TA Hesse, CN Sneh, B Stockwell, VO Taylor, BJ AF Loper, Joyce E. Henkels, Marcella D. Rangel, Lorena I. Olcott, Marika H. Walker, Francesca L. Bond, Kise L. Kidarsa, Teresa A. Hesse, Cedar N. Sneh, Baruch Stockwell, Virginia O. Taylor, Barbara J. TI Rhizoxin analogs, orfamide A and chitinase production contribute to the toxicity of Pseudomonas protegens strain Pf-5 to Drosophila melanogaster SO ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID ANTIFUNGAL METABOLITE PRODUCTION; PLANT-ASSOCIATED PSEUDOMONADS; FLUORESCENS PF-5; BIOLOGICAL-CONTROL; GENE-CLUSTER; PERITROPHIC MATRIX; COTTON SEEDLINGS; INSECT TOXIN; DAMPING-OFF; IDENTIFICATION AB Pseudomonas protegens strain Pf-5 is a soil bacterium that was first described for its capacity to suppress plant diseases and has since been shown to be lethal to certain insects. Among these is the common fruit fly Drosophila melanogaster, a well-established model organism for studies evaluating the molecular and cellular basis of the immune response to bacterial challenge. Pf-5 produces the insect toxin FitD, but a Delta fitD mutant of Pf-5 retained full toxicity against D. melanogaster in a noninvasive feeding assay, indicating that FitD is not a major determinant of Pf-5's oral toxicity against this insect. Pf-5 also produces a broad spectrum of exoenzymes and natural products with antibiotic activity, whereas a mutant with a deletion in the global regulatory gene gacA produces none of these exoproducts and also lacks toxicity to D. melanogaster. In this study, we made use of a panel of Pf-5 mutants having single or multiple mutations in the biosynthetic gene clusters for seven natural products and two exoenzymes that are produced by the bacterium under the control of gacA. Our results demonstrate that the production of rhizoxin analogs, orfamide A, and chitinase are required for full oral toxicity of Pf-5 against D. melanogaster, with rhizoxins being the primary determinant. C1 [Loper, Joyce E.; Henkels, Marcella D.; Kidarsa, Teresa A.; Hesse, Cedar N.] ARS, USDA, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. [Loper, Joyce E.; Henkels, Marcella D.; Rangel, Lorena I.; Kidarsa, Teresa A.; Stockwell, Virginia O.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Olcott, Marika H.; Walker, Francesca L.; Bond, Kise L.; Taylor, Barbara J.] Oregon State Univ, Dept Integrat Biol, Corvallis, OR 97331 USA. [Sneh, Baruch] Tel Aviv Univ, Dept Mol Biol & Ecol Plants, George S Wise Fac Life Sci, Tel Aviv, Israel. RP Loper, JE (reprint author), ARS, USDA, 3420 NW Orchard Ave, Corvallis, OR 97330 USA.; Loper, JE (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM loperj@science.oregonstate.edu OI Loper, Joyce/0000-0003-3501-5969 FU Agriculture and Food Research Initiative Competitive Grants from the United States Department of Agriculture National Institute of Food and Agriculture [2006-35319-17427, 2011-67019-30192] FX We gratefully acknowledge the assistance of Brenda Shaffer, Ashley Bixenstein and Max Kohen in carrying out the experiments described herein. We also thank Donald Kobayashi of Rutgers, the State University of New Jersey, for providing a chiC mutant of Pf-5 for this study. This work was supported by Agriculture and Food Research Initiative Competitive Grants 2006-35319-17427 and 2011-67019-30192 from the United States Department of Agriculture National Institute of Food and Agriculture. NR 69 TC 3 Z9 3 U1 1 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1462-2912 EI 1462-2920 J9 ENVIRON MICROBIOL JI Environ. Microbiol. PD OCT PY 2016 VL 18 IS 10 SI SI BP 3509 EP 3521 DI 10.1111/1462-2920.13369 PG 13 WC Microbiology SC Microbiology GA ED1NW UT WOS:000388613200023 PM 27130686 ER PT J AU Barnett, K Parks, SA Miller, C Naughton, HT AF Barnett, Kevin Parks, Sean A. Miller, Carol Naughton, Helen T. TI Beyond Fuel Treatment Effectiveness: Characterizing Interactions between Fire and Treatments in the US SO FORESTS LA English DT Article DE encounter rate; treatment maintenance; treatment longevity; MTBS; LANDFIRE; wildland fire ID WILDLAND-URBAN INTERFACE; WESTERN UNITED-STATES; SOUTHERN SIERRA-NEVADA; MIXED-CONIFER FOREST; BURNED AREA; WILDFIRE; CALIFORNIA; LANDSCAPE; SEVERITY; PROBABILITY AB In the United States, fuel reduction treatments are a standard land management tool to restore the structure and composition of forests that have been degraded by past management. Although treatments can have multiple purposes, their principal objective is to create landscape conditions where wildland fire can be safely managed to help achieve long-term land management goals. One critique is that fuel treatment benefits are unlikely to transpire due to the low probability that treated areas will be burned by a subsequent fire within a treatment's lifespan, but little quantitative information exists to corroborate this argument. We summarized the frequency, extent, and geographic variation of fire and fuel treatment interactions on federal lands within the conterminous United States (CONUS). We also assessed how the encounters between fuel treatments and fires varied with treatment size, treatment age, and number of times treated. Overall, 6.8% of treatment units evaluated were encountered by a subsequent fire during the study period, though this rate varied among ecoregions across the CONUS. Larger treatment units were more likely to be encountered by a fire, and treatment units were most frequently burned within one year of the most recent treatment, the latter of which is likely because of ongoing maintenance of existing treatments. Our results highlight the need to identify and prioritize additional opportunities to reduce fuel loading and fire risk on the millions of hectares of federal lands in the CONUS that are in need of restoration. C1 [Barnett, Kevin; Naughton, Helen T.] Univ Montana, Dept Econ, Missoula, MT 59701 USA. [Parks, Sean A.; Miller, Carol] US Forest Serv, Aldo Leopold Wilderness Res Inst, Rocky Mt Res Stn, USDA, Missoula, MT 59801 USA. RP Barnett, K (reprint author), Univ Montana, Dept Econ, Missoula, MT 59701 USA. EM kevin.barnett@umontana.edu; sean_parks@fs.fed.us; cmiller04@fs.fed.us; helen.naughton@mso.umt.edu FU Joint Fire Science Program [14-5-01-25] FX We thank two anonymous reviewers for thoughtful comments that significantly improved the manuscript. Funding for this research was provided by the Joint Fire Science Program under Project 14-5-01-25. NR 55 TC 0 Z9 0 U1 3 U2 3 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD OCT PY 2016 VL 7 IS 10 AR 237 DI 10.3390/f7100237 PG 12 WC Forestry SC Forestry GA ED2JQ UT WOS:000388670800028 ER PT J AU Dang, M Zhang, T Hu, YH Zhou, HJ Woeste, KE Zhao, P AF Dang, Meng Zhang, Tian Hu, Yiheng Zhou, Huijuan Woeste, Keith E. Zhao, Peng TI De Novo Assembly and Characterization of Bud, Leaf and Flowers Transcriptome from Juglans Regia L. for the Identification and Characterization of New EST-SSRs SO FORESTS LA English DT Article DE microsatellites; transcriptome; next-generation sequencing; genetic diversity; English walnut ID RNA-SEQ DATA; DATA PROVIDES INSIGHT; MICROSATELLITE MARKERS; GENETIC DIVERSITY; DROUGHT RESPONSE; WALNUT CULTIVARS; ANNOTATION; DISCOVERY; SOFTWARE; PROGRAM AB Persian walnut (Juglans regia L.), valued for both its nut and wood, is an ecologically important temperate tree species native to the mountainous regions of central Asia. Despite its importance, there are still few transcriptomic resources in public databases for J. regia, limiting gene discovery and breeding. Here, more than 49.9 million sequencing reads were generated using Illumina sequencing technology in the characterization of the transcriptome of four J. regia organs (bud, leaf, female flowers, and male flowers). De novo assembly yielded 117,229 unigenes with an N50 of 1955 bp. Based on sequence similarity searches against known proteins, a total of 20,413 (17.41%) genes were identified and annotated. A set of 27,584 unigenes with SSR (simple sequence repeats) motifs were identified as potential molecular markers, and a sample of 77 of these EST-SSRs (express sequence tags) were further evaluated to validate their amplification and assess their polymorphism. Next, we developed 39 polymorphic microsatellite markers to screen 88 Persian walnut individuals collected from 11 populations. These markers and transcriptomic resources will be useful for future studies of population genetic structure, evolutionary ecology, and breeding of Persian walnut and other Juglans species. C1 [Dang, Meng; Zhang, Tian; Hu, Yiheng; Zhou, Huijuan; Zhao, Peng] Northwest Univ, Key Lab Resource Biol & Biotechnol Western China, Minist Educ, Coll Life Sci, Xian 710069, Shanxi, Peoples R China. [Woeste, Keith E.] Purdue Univ, USDA, Forest Serv, HTIRC,Dept Forestry & Nat Resources, 715 West State St, W Lafayette, IN 47907 USA. RP Zhao, P (reprint author), Northwest Univ, Key Lab Resource Biol & Biotechnol Western China, Minist Educ, Coll Life Sci, Xian 710069, Shanxi, Peoples R China.; Woeste, KE (reprint author), Purdue Univ, USDA, Forest Serv, HTIRC,Dept Forestry & Nat Resources, 715 West State St, W Lafayette, IN 47907 USA. EM 15339260798@163.com; 13239166791@163.com; berlin@stumail.nwu.edu.cn; nandehuturen@163.com; woeste@purdue.edu; pengzhao@nwu.edu.cn FU National Natural Science Foundation of China [31200500, 41471038, J1210063]; Changjiang Scholars and Innovative Research Team in University [IRT1174]; Program for Excellent Young Academic Backbones - Northwest University [338050070]; Northwest University Training Programs of Innovation and Entrepreneurship for Undergraduates [2015159, 2016171] FX The authors wish to thank Jia Yang, Li Feng, Hailong Xia, Qiang Zhang, and Tao Zhou for sample collection. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that also may be suitable. This work was supported by the National Natural Science Foundation of China (Grant No. 31200500; Grant No. 41471038; Grant No. J1210063), Changjiang Scholars and Innovative Research Team in University (No. IRT1174), the Program for Excellent Young Academic Backbones funding by Northwest University (Grant No. 338050070), and the Northwest University Training Programs of Innovation and Entrepreneurship for Undergraduates (Grant No. 2015159 and Grand No. 2016171). NR 61 TC 0 Z9 0 U1 13 U2 13 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD OCT PY 2016 VL 7 IS 10 AR 247 DI 10.3390/f7100247 PG 17 WC Forestry SC Forestry GA ED2JQ UT WOS:000388670800037 ER PT J AU Marschall, JM Stambaugh, MC Jones, BC Guyette, RP Brose, PH Dey, DC AF Marschall, Joseph M. Stambaugh, Michael C. Jones, Benjamin C. Guyette, Richard P. Brose, Patrick H. Dey, Daniel C. TI Fire Regimes of Remnant Pitch Pine Communities in the Ridge and Valley Region of Central Pennsylvania, USA SO FORESTS LA English DT Article DE Pennsylvania; dendrochronology; fire scars; fire severity; humans ID EASTERN UNITED-STATES; NORTH-CENTRAL PENNSYLVANIA; LAND-USE; AMERICAN FORESTS; PRESCRIBED FIRE; OAK FORESTS; HISTORY; CLIMATE; MOUNTAINS; DYNAMICS AB Many fire-adapted ecosystems in the northeastern U.S. are converting to fire-intolerant vegetation communities due to fire suppression in the 20th century. Prescribed fire and other vegetation management activities that increase resilience and resistance to global changes are increasingly being implemented, particularly on public lands. For many fire-dependent communities, there is little quantitative data describing historical fire regime attributes such as frequency, severity, and seasonality, or how these varied through time. Where available, fire-scarred live and remnant trees, including stumps and snags, offer valuable insights into historical fire regimes through tree-ring and fire-scar analyses. In this study, we dated fire scars from 66 trees at two sites in the Ridge and Valley Province of the Appalachian Mountains in central Pennsylvania, and described fire frequency, severity, and seasonality from the mid-17th century to 2013. Fires were historically frequent, of low to moderate severity, occurred mostly during the dormant season, and were influenced by aspect and topography. The current extended fire-free interval is unprecedented in the previous 250-300 years at both sites. C1 [Marschall, Joseph M.; Stambaugh, Michael C.; Guyette, Richard P.] Univ Missouri, Sch Nat Resources, Missouri Tree Ring Lab, 203 ABNR Bldg, Columbia, MO 65211 USA. [Jones, Benjamin C.] Penn Game Commiss, Habitat Planning & Dev Div, Harrisburg, PA 17110 USA. [Brose, Patrick H.; Dey, Daniel C.] US Forest Serv, Northern Res Stn, Newtown Sq, PA 19073 USA. RP Marschall, JM (reprint author), Univ Missouri, Sch Nat Resources, Missouri Tree Ring Lab, 203 ABNR Bldg, Columbia, MO 65211 USA. EM marschallj@missouri.edu; stambaughm@missouri.edu; benjjones@pa.gov; guyetter@missouri.edu; pbrose@fs.fed.us; ddey@fs.fed.us FU U.S. Forest Service, Northern Research Station; Pennsylvania Game Commission FX Identification of study sites was supported by the U.S. Forest Service, Northern Research Station. This research was funded by the Pennsylvania Game Commission. We thank David Matthew Bourscheidt for data collection assistance in the field, Erin Abadir for help identifying cultural and land-use periods, and Chris Bobryk for cartography assistance. We are grateful for contributions from two anonymous reviewers, which improved the manuscript. NR 80 TC 0 Z9 0 U1 6 U2 6 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD OCT PY 2016 VL 7 IS 10 AR 224 DI 10.3390/f7100224 PG 16 WC Forestry SC Forestry GA ED2JQ UT WOS:000388670800015 ER PT J AU Corson-Rikert, HA Wondzell, SM Haggerty, R Santelmann, MV AF Corson-Rikert, Hayley A. Wondzell, Steven M. Haggerty, Roy Santelmann, Mary V. TI Carbon dynamics in the hyporheic zone of a headwater mountain stream in the Cascade Mountains, Oregon SO WATER RESOURCES RESEARCH LA English DT Article DE hyporheic; dissolved inorganic carbon; dissolved organic carbon ID DISSOLVED ORGANIC-CARBON; VERTICAL HYDROLOGIC EXCHANGE; INORGANIC CARBON; WATER CHEMISTRY; METABOLISM; FLUXES; FOREST; FLOW; SOIL; GROUNDWATER AB We investigated carbon dynamics in the hyporheic zone of a steep, forested, headwater catchment western Oregon, USA. Water samples were collected monthly from the stream and a well network during base flow periods. We examined the potential for mixing of different source waters to explain concentrations of DOC and DIC. We did not find convincing evidence that either inputs of deep groundwater or lateral inputs of shallow soil water influenced carbon dynamics. Rather, carbon dynamics appeared to be controlled by local processes in the hyporheic zone and overlying riparian soils. DOC concentrations were low in stream water (0.04-0.09 mM), and decreased with nominal travel time through the hyporheic zone (0.02-0.04 mM lost over 100 h). Conversely, stream water DIC concentrations were much greater than DOC (0.35-0.7 mM) and increased with nominal travel time through the hyporheic zone (0.2-0.4 mM gained over 100 h). DOC in stream water could only account for 10% of the observed increase in DIC. In situ metabolic processing of buried particulate organic matter as well as advection of CO2 from the vadose zone likely accounted for the remaining 90% of the increase in DIC. Overall, the hyporheic zone was a source of DIC to the stream. We suggest that, in mountain stream networks, hyporheic exchange facilitates the transformation of particulate organic carbon buried in floodplains and transports the DIC that is produced back to the stream where it can be evaded to the atmosphere. C1 [Corson-Rikert, Hayley A.; Haggerty, Roy; Santelmann, Mary V.] Oregon State Univ, Coll Earth Ocean & Atmospher Sci, Corvallis, OR USA. [Wondzell, Steven M.] US Forest Serv, Pacific Northwest Res Stn, Corvallis, OR USA. RP Corson-Rikert, HA (reprint author), Oregon State Univ, Coll Earth Ocean & Atmospher Sci, Corvallis, OR USA. EM hacorrik@gmail.com RI Haggerty, Roy/A-5863-2009 FU U.S. Forest Service Pacific Northwest Research Station; Water Resources Graduate Program; Oregon State University Provost's Distinguished Fellowship; National Science Foundation [EAR 1417603]; National Science Foundation's Long-Term Ecological Research Program [DEB 0823380]; Oregon State University; [10-JV-11261991-055-PNW] FX Following publication, all data will be made available through the H.J. Andrews Experimental Forest data catalog, which can be accessed via the following url: http://andrewsforest.oregonstate.edu/lter/data.cfm?frameURL=8. We also cited unpublished data collected by Serchan and Pennington. Satish Serchan can be reached at satish.serchan@gmail.com, and Robert Pennington can be reached at robertopennington@gmail.com. Direct support for this work was provided by agreement 10-JV-11261991-055-PNW. Additional funding and support for this research was provided by the U.S. Forest Service Pacific Northwest Research Station, the Water Resources Graduate Program, the Oregon State University Provost's Distinguished Fellowship, and the National Science Foundation (EAR 1417603). Data and research facilities were provided by the H.J. Andrews Experimental Forest research program, which is funded by the National Science Foundation's Long-Term Ecological Research Program (DEB 0823380), the U.S. Forest Service Pacific Northwest Research Station, and Oregon State University. We thank Nick Dosch for help with fieldwork, Lisa Ganio for help with data analysis, Noah Schmadel and Adam Ward who provided analyses of watershed drainage areas and UAA, both the H.J. Andrews staff and the LTER research network for data and field support, and the IWW Collaboratory and Kathy Motter for laboratory equipment and training, as well as technical support. NR 66 TC 0 Z9 0 U1 14 U2 14 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD OCT PY 2016 VL 52 IS 10 BP 7556 EP 7576 DI 10.1002/2016WR019303 PG 21 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA EC9VW UT WOS:000388493400002 ER PT J AU Miller, MP Boyer, EW McKnight, DM Brown, MG Gabor, RS Hunsaker, CT Iavorivska, L Inamdar, S Johnson, DW Kaplan, LA Lin, H McDowell, WH Perdrial, JN AF Miller, Matthew P. Boyer, Elizabeth W. McKnight, Diane M. Brown, Michael G. Gabor, Rachel S. Hunsaker, Carolyn T. Iavorivska, Lidiia Inamdar, Shreeram Johnson, Dale W. Kaplan, Louis A. Lin, Henry McDowell, William H. Perdrial, Julia N. TI Variation of organic matter quantity and quality in streams at Critical Zone Observatory watersheds SO WATER RESOURCES RESEARCH LA English DT Article DE DOM; critical zone; atmospheric deposition; fluorescence ID NORTHEASTERN UNITED-STATES; ECOSYSTEM METABOLISM; CHEMICAL-COMPOSITION; ACID DEPOSITION; CARBON-CYCLE; FULVIC-ACIDS; SOILS; CATCHMENT; LAKES; BIODEGRADABILITY AB The quantity and chemical composition of dissolved organic matter (DOM) in surface waters influence ecosystem processes and anthropogenic use of freshwater. However, despite the importance of understanding spatial and temporal patterns in DOM, measures of DOM quality are not routinely included as part of large-scale ecosystem monitoring programs and variations in analytical procedures can introduce artifacts. In this study, we used consistent sampling and analytical methods to meet the objective of defining variability in DOM quantity and quality and other measures of water quality in streamflow issuing from small forested watersheds located within five Critical Zone Observatory sites representing contrasting environmental conditions. Results show distinct separations among sites as a function of water quality constituents. Relationships among rates of atmospheric deposition, water quality conditions, and stream DOM quantity and quality are consistent with the notion that areas with relatively high rates of atmospheric nitrogen and sulfur deposition and high concentrations of divalent cations result in selective transport of DOM derived from microbial sources, including in-stream microbial phototrophs. We suggest that the critical zone as a whole strongly influences the origin, composition, and fate of DOM in streams. This study highlights the value of consistent DOM characterization methods included as part of long-term monitoring programs for improving our understanding of interactions among ecosystem processes as controls on DOM biogeochemistry. C1 [Miller, Matthew P.; Boyer, Elizabeth W.; Brown, Michael G.; Iavorivska, Lidiia; Lin, Henry] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [Miller, Matthew P.] US Geol Survey, Utah Water Sci Ctr, Salt Lake City, UT USA. [McKnight, Diane M.; Gabor, Rachel S.] Univ Colorado, Dept Civil Environm & Architectural Engn, Boulder, CO 80309 USA. [Hunsaker, Carolyn T.] US Forest Serv, USDA, Pacific Southwest Res Stn, Fresno, CA USA. [Inamdar, Shreeram] Univ Delaware, Dept Plant & Soil Sci, Newark, DE 19717 USA. [Johnson, Dale W.] Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89557 USA. [Kaplan, Louis A.] Stroud Water Res Ctr, Avondale, PA USA. [McDowell, William H.] Univ New Hampshire, Dept Nat Resources & Environm, Durham, NH 03824 USA. [Perdrial, Julia N.] Univ Vermont, Dept Geol, Burlington, VT USA. RP Miller, MP (reprint author), Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA.; Miller, MP (reprint author), US Geol Survey, Utah Water Sci Ctr, Salt Lake City, UT USA. EM mamiller@usgs.gov RI McDowell, William/E-9767-2010; Lin, Henry/E-8234-2011; OI McDowell, William/0000-0002-8739-9047; Iavorivska, Lidiia/0000-0002-6224-6271 FU National Science Foundation [Southern Sierra: EAR-0725097, Boulder: EAR-0724960, Shale Hills: EAR-0725019, EAR12-39285, EAR-1331726, Christina River EAR-0724971, EAR-0809205, Luquillo: EAR-0722476, EAR-1331841] FX We thank Eric Parrish, Matt Meadows, Shatrughan Singh, John Bithorn, Jeff Grimm, and Tim White for technical assistance. Helpful comments on an earlier draft of this manuscript were provided by Douglas Burns and three anonymous reviewers. This work was supported in part by the National Science Foundation (Southern Sierra: EAR-0725097; Boulder: EAR-0724960; Shale Hills: EAR-0725019, EAR12-39285, EAR-1331726; Christina River EAR-0724971, EAR-0809205; Luquillo: EAR-0722476, EAR-1331841). All data from this paper are included here as Supporting Information. NR 83 TC 0 Z9 0 U1 12 U2 12 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD OCT PY 2016 VL 52 IS 10 BP 8202 EP 8216 DI 10.1002/2016WR018970 PG 15 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA EC9VW UT WOS:000388493400038 ER PT J AU Hernandez-Garcia, AD Columbus, DA Manjarin, R Nguyen, HV Suryawan, A Orellana, RA Davis, TA AF Hernandez-Garcia, Adriana D. Columbus, Daniel A. Manjarin, Rodrigo Nguyen, Hanh V. Suryawan, Agus Orellana, Renan A. Davis, Teresa A. TI Leucine supplementation stimulates protein synthesis and reduces degradation signal activation in muscle of newborn pigs during acute endotoxemia SO AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM LA English DT Article DE leucine; skeletal muscle; protein synthesis; protein degradation; autophagy ID AMINO-ACID AVAILABILITY; PEDIATRIC SEVERE SEPSIS; SKELETAL-MUSCLE; NEONATAL PIGS; TRANSLATION INITIATION; DIFFERENTIAL REGULATION; POSTABSORPTIVE RATS; MAMMALIAN TARGET; IGF-I; INSULIN AB Sepsis disrupts skeletal muscle proteostasis and mitigates the anabolic response to leucine (Leu) in muscle of mature animals. We have shown that Leu stimulates muscle protein synthesis (PS) in healthy neonatal piglets. To determine if supplemental Leu can stimulate PS and reduce protein degradation (PD) signaling in neonatal muscle during endotoxemia, overnight-fasted neonatal pigs were infused for 8 h with LPS or saline while plasma amino acids, glucose, and insulin were maintained at fasting levels during pancreatic-substrate clamps. Leu or saline was infused during the last hour. Markers of PS and PD were determined in skeletal muscle. Compared with controls, Leu increased PS in longissimus dorsi (LD), gastrocnemius, and soleus muscles. LPS decreased PS in these three muscles by 36%, 28%, and 38%, but Leu antagonized that reduction by increasing PS by 84%, 81%, and 83%, respectively, when supplemented to LPS. Leu increased eukaryotic translation initiation factor (eIF)3b-raptor interactions, eIF4E-binding protein-1, and S6 kinase 1 phosphorylation as well as eIF4E-eIF4G complex formation in LD, gastrocnemius, and soleus muscles of control and LPS-treated pigs. In LD muscle, LPS increased the light chain (LC)3-II-to-LC3 ratio and muscle-specific RING finger (MuRF-1) abundance but not atrogin-1 abundance or AMP-activated protein kinase-alpha phosphorylation. Leu supplementation to LPS-treated pigs reduced the LC3-II-to-LC3 ratio, MuRF-1 abundance, and AMP-activated protein kinase-alpha phosphorylation compared with LPS alone. In conclusion, parenteral Leu supplementation attenuates the LPS-induced reduction in PS by stimulating mammalian target of rapamycin complex 1-dependent translation and may reduce PD by attenuating autophagy-lysosome and MuRF-1 signaling in neonatal skeletal muscle. C1 [Hernandez-Garcia, Adriana D.; Columbus, Daniel A.; Manjarin, Rodrigo; Nguyen, Hanh V.; Suryawan, Agus; Orellana, Renan A.; Davis, Teresa A.] USDA ARS, Childrens Nutr Res Ctr, Houston, TX USA. [Hernandez-Garcia, Adriana D.; Orellana, Renan A.] Baylor Coll Med, Dept Pediat, Crit Care Sect, Houston, TX 77030 USA. RP Davis, TA (reprint author), Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM tdavis@bcm.edu FU National Institutes of Health [HD-072891, AR-044474, HD-085573]; USDA NIFA [2013-67015-20438]; USDA CRIS [6250-51000-055] FX This work is a publication of the Children's Nutrition Research Center, United States Department of Agriculture (USDA)/Agricultural Research Service, and Department of Pediatrics, Baylor College of Medicine. Financial support for this project was provided by National Institutes of Health Grants HD-072891 (to T. A. Davis), AR-044474 (to T. A. Davis), and HD-085573 (to T. A. Davis) as well as USDA NIFA 2013-67015-20438 (to T. A. Davis) and USDA CRIS 6250-51000-055 (to T. A. Davis). NR 53 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1849 EI 1522-1555 J9 AM J PHYSIOL-ENDOC M JI Am. J. Physiol.-Endocrinol. Metab. PD OCT 1 PY 2016 VL 311 IS 4 BP E791 EP E801 DI 10.1152/ajpendo.00217.2016 PG 11 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA EC9IB UT WOS:000388457000015 PM 27624100 ER PT J AU Broadhurst, CL Schmidt, WF Kim, MS Nguyen, JK Qin, JW Chao, KL Bauchan, GL Shelton, DR AF Broadhurst, C. Leigh Schmidt, Walter F. Kim, Moon S. Nguyen, Julie K. Qin, Jianwei Chao, Kuanglin Bauchan, Gary L. Shelton, Daniel R. TI Continuous gradient temperature Raman spectroscopy of N-6DPA and DHA from-100 to 20 degrees C SO CHEMISTRY AND PHYSICS OF LIPIDS LA English DT Article DE Gradient temperature Raman spectroscopy; n-6DPA; DHA; n-6 Docosapentaenoic acid; Docosahexaenoic acid; Raman spectroscopy ID DOCOSAHEXAENOIC ACID; DOCOSAPENTAENOIC ACID; BRAIN-DEVELOPMENT; OLEIC-ACID; DPA N-3; OMEGA-3-FATTY-ACIDS; CHOLESTEROL; EVOLUTION; SPECTRA; RETINA AB One of the great unanswered questions with respect to biological science in general is the absolute necessity of docosahexaenoic acid (DHA, 22:6n-3) in fast signal processing tissues. N-6 docosapentaenoic acid (n-6DPA, 22:5n-6), with just one less double bond, group, is fairly abundant in terrestrial food chains yet cannot substitute for DHA. Gradient temperature Raman spectroscopy (GTRS) applies the temperature gradients utilized in differential scanning calorimetry (DSC) to Raman spectroscopy, providing a straightforward technique to identify molecular rearrangements that occur near and at phase transitions. Herein we apply GTRS and DSC to n-6DPA and DHA from -100 to 20 degrees C. 20 Mb three-dimensional data arrays with 0.2 degrees C increments and first/second derivatives allowed complete assignment of solid, liquid and transition state vibrational modes, including low intensity/frequency vibrations that cannot be readily analyzed with conventional Raman. N-6DPA and DHA show significant spectral changes with premelting (-33 and -60 degrees C, respectively) and melting (-27 and -44 degrees C, respectively). The CH2-(HC=CH)-CH2 moieties are not identical in the second half of the DHA and DPA structures. DPA has bending (1450 cm(-1)) over almost the entire temperature range. In contrast, DHA contains major CH2 twisting (1265 cm(-1)) with no noticeable CH2 bending, consistent with a flat helical structure with a small pitch. Further modeling of neuronal membrane phospholipids must take into account torsion present in the DHA structure, which essential in determining whether the lipid chain is configured more parallel or perpendicular to the hydrophilic head group. (C) 2016 Published by Elsevier Ireland Ltd. C1 [Broadhurst, C. Leigh; Schmidt, Walter F.; Kim, Moon S.; Nguyen, Julie K.; Qin, Jianwei; Chao, Kuanglin; Shelton, Daniel R.] USDA ARS, Environm Microbiol & Food Safety Lab, Bldg 161 Rm 116,10300 Baltimore Ave, Beltsville, MD 20705 USA. [Broadhurst, C. Leigh] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Bauchan, Gary L.] USDA ARS, Electron & Confocal Microscopy Unit, Inst Plant Sci, Beltsville, MD USA. RP Broadhurst, CL (reprint author), USDA ARS, Environm Microbiol & Food Safety Lab, Bldg 161 Rm 116,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM leigh.broadhurst@ars.usda.gov NR 33 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0009-3084 EI 1873-2941 J9 CHEM PHYS LIPIDS JI Chem. Phys. Lipids PD OCT PY 2016 VL 200 BP 1 EP 10 DI 10.1016/j.chemphyslip.2016.06.003 PG 10 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA EC5DI UT WOS:000388152900001 PM 27326703 ER PT J AU Asiimwe, P Ellsworth, PC Naranjo, SE AF Asiimwe, Peter Ellsworth, Peter C. Naranjo, Steven E. TI Natural enemy impacts on Bemisia tabaci (MEAM1) dominate plant quality effects in the cotton system SO ECOLOGICAL ENTOMOLOGY LA English DT Article DE Arthropod predators; biological control; life tables; parasitoids; plant stress; whiteflies ID BOTTOM-UP FORCES; LIFE-TABLE ANALYSIS; ARGENTIFOLII HOMOPTERA ALEYRODIDAE; CONTEMPORANEOUS MORTALITY FACTORS; TOP-DOWN CONTROL; INSECT POPULATIONS; PARASITOID WASP; RELATIVE ROLES; WATER-STRESS; KEY FACTORS AB 1. Plant quality (bottom-up effects) and natural enemies (top-down effects) affect herbivore performance. Furthermore, plant quality can also influence the impact of natural enemies. 2. Lower plant quality through reduced irrigation increased the abundance of the cryptic species from the Bemisia tabaci complex [hereafter B. tabaci Middle East Asia Minor 1 (MEAM1)], but not its natural enemies on cotton. It was therefore predicted that lower plant quality would diminish the impact of natural enemies in regulating this herbivore. 3. Over three cotton seasons, plant quality was manipulated via differential irrigation and natural enemy abundance with insecticides. Life tables were used to evaluate the impact of these factors on mortality of immature B. tabaci (MEAM1) over nine generations. 4. Mortality of B. tabaci (MEAM1) was consistently affected by natural enemies but not by plant quality. This pattern was driven by high levels of sucking predation, which was the primary (key) factor associated with changes in immature mortality across all irrigation and natural enemy treatments. Dislodgement (chewing predation and weather) and parasitism contributed as key factors in some cases. Analyses also showed that elimination of sucking predation and dislodgement would have the greatest effect on overall mortality. 5. The top-down effects of natural enemies had dominant effects on populations of B. tabaci (MEAM1) relative to the bottom-up effects of plant quality. Effects were primarily due to native generalist arthropod predators and not more host-specific aphelinid parasitoids. The findings of this study demonstrate the important role of arthropod predators in population suppression and validate the importance of conservation biological control in this system for effective pest control. C1 [Asiimwe, Peter; Ellsworth, Peter C.] Univ Arizona, Maricopa Agr Ctr, Dept Entomol, Maricopa, AZ USA. [Naranjo, Steven E.] USDA ARS, Arid Land Agr Res Ctr, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. [Asiimwe, Peter] Monsanto Co, 800 N Lindbergh Blvd, St Louis, MO 63167 USA. RP Naranjo, SE (reprint author), USDA ARS, Arid Land Agr Res Ctr, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. EM naranjo@ars.usda.gov OI Ellsworth, Peter/0000-0002-2485-0830 FU Maricopa Agricultural Center; Arizona Pest Management Center; USDA-National Institute for Food and Agriculture Extension IPM program; USDA-ARS; Cotton Incorporated; Arizona Cotton Growers Association; Cotton Foundations FX We thank Y. Carriere, G. Wang and R. Kaggwa for helpful comments on earlier drafts of this manuscript. Ed Martin provided guidance on establishing the irrigation regimes. M. Stefanek, V. Barkley, G. Castro, F. Bojorquez, B. Stuart, J. Trejo, A. Slade, L. Rodarte and A. Sonoqui provided expert field and laboratory technical assistance. We also appreciate the support of the Maricopa Agricultural Center, the Arizona Pest Management Center including a grant from the USDA-National Institute for Food and Agriculture Extension IPM program, the USDA-ARS, Cotton Incorporated, the Arizona Cotton Growers Association, and the Cotton Foundations. NR 91 TC 0 Z9 0 U1 13 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0307-6946 EI 1365-2311 J9 ECOL ENTOMOL JI Ecol. Entomol. PD OCT PY 2016 VL 41 IS 5 BP 642 EP 652 DI 10.1111/een.12340 PG 11 WC Entomology SC Entomology GA EC7HU UT WOS:000388308600016 ER PT J AU Swift, SM Rowley, DT Young, C Franks, A Hyman, P Donovan, DM AF Swift, Steven M. Rowley, D. Treva Young, Carly Franks, Ashley Hyman, Paul Donovan, David M. TI The endolysin from the Enterococcus faecalis bacteriophage VD13 and conditions stimulating its lytic activity SO FEMS MICROBIOLOGY LETTERS LA English DT Article DE VD13; endolysin; Enterococcus faecalis; peptidoglycan hydrolase ID LEVEL GLYCOPEPTIDE RESISTANCE; COMPLETE GENOME SEQUENCE; WALL BINDING DOMAIN; CRYSTAL-STRUCTURE; CLOSTRIDIUM-PERFRINGENS; ANIMAL HUSBANDRY; FECAL SAMPLES; FAECIUM; POULTRY; LYSIS AB Bacteriophages produce endolysins (peptidoglycan hydrolases) to lyse the host cell from within and release nascent bacteriophage particles. Recombinant endolysins can lyse Gram-positive bacteria when added exogenously. As a potential alternative antimicrobial, we cloned and expressed the enterococcal VD13 bacteriophage endolysin. VD13 endolysin has a CHAP catalytic domain with 92% identity with the bacteriophage IME-EF1 endolysin. The predicted size of VD13 endolysin is similar to 27 kDa as verified by SDS-PAGE. The VD13 endolysin lyses Enterococcus faecalis strains, but not E. faecium or other non-enterococci. VD13 endolysin has activity from pH 4 to pH 8, with peak activity at pH 5, and exhibits greater activity in the presence of calcium. Optimum activity at pH 5 occurs in the absence of NaCl. VD13 endolysin, in ammonium acetate (C2H3O2NH4) calcium chloride (CaCl2) buffer pH 5, is stimulated to higher activity upon heating at temperatures up to 65 degrees C for 30 min, whereas activity is lost upon heating to 42 degrees C, in pH 7 buffer. C1 [Swift, Steven M.; Rowley, D. Treva; Donovan, David M.] ARS, Anim Biosci & Biotechnol Lab, NEA, USDA, 10300 Baltimore Ave,Bldg 230 Barc East, Beltsville, MD 20705 USA. [Young, Carly; Franks, Ashley; Hyman, Paul] Ashland Univ, Biol & Toxicol Dept, Ashland, OH 44805 USA. RP Donovan, DM (reprint author), ARS, Anim Biosci & Biotechnol Lab, NEA, USDA, 10300 Baltimore Ave,Bldg 230 Barc East, Beltsville, MD 20705 USA. EM david.donovan@ars.usda.gov FU Choose Ohio First; AU Provost Student Research grant; BARD Grant [IS-4573-12R] FX Carly Young was partly supported by Choose Ohio First funding and an AU Provost Student Research grant. D. Treva Rowley was supported by BARD Grant no. IS-4573-12R to DD. NOTE: Mention of trade names or commercial products is solely for providing specific information and does not imply recommendation or endorsement by the ARS, USDA. NR 39 TC 0 Z9 0 U1 3 U2 3 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0378-1097 EI 1574-6968 J9 FEMS MICROBIOL LETT JI FEMS Microbiol. Lett. PD OCT PY 2016 VL 363 IS 19 AR fnw216 DI 10.1093/femsle/fnw216 PG 8 WC Microbiology SC Microbiology GA EC8HI UT WOS:000388381800008 ER PT J AU Bowman, KD Faulkner, L Kesinger, M AF Bowman, Kim D. Faulkner, Lynn Kesinger, Mike TI New Citrus Rootstocks Released by USDA 2001-2010: Field Performance and Nursery Characteristics SO HORTSCIENCE LA English DT Article DE orange; grapefruit; mandarin; lemon; lime; huanglongbing; Citrus tristeza virus; phytophthora; diaprepes; disease; cold; cultivar; US-802; US-812; US-897; US-942 ID SINENSIS L. OSBECK; LIBERIBACTER ASIATICUS; VALENCIA ORANGE; CENTRAL FLORIDA; TOLERANCE; HYBRIDS; BLIGHT; TREE AB Four new citrus rootstocks developed by USDA, ARS and released between 2001 and 2010 have gained considerable commercial popularity in Florida and have been used for propagation of more than 2 million trees over the last 2 years. Results from three new field trials are presented comparing these rootstocks with other important rootstocks, and discussion is presented to summarize field performance in these trials and from numerous other sources. The rootstocks vary widely in their effect on tree vigor. When used as a rootstock for sweet orange, 'US-802' typically supports strong vigor and development of a large tree, 'US-812' and 'US-942' provide moderate vigor, and 'US-897' induces a relatively dwarf tree. Other characteristics and attributes of the four rootstocks are also discussed, including effects on cropping, fruit quality, disease and pest resistance, and tolerance of abiotic factors. Of special relevance, relative tolerance of these rootstocks to huanglongbing (HLB) disease can be surmised from a combination of data from several sources, with 'US-942' and 'US-802' typically providing the best yields per tree in trials containing many rootstocks and affected by HLB. 'US-812' and 'US-897' have appeared to have most utility in locations where HLB is not present or effectively managed, or where trees are being planted at high density to optimize production per unit area. To facilitate continuing expansion of commercial use, information on seed production, and the relative ease of nursery propagation is also presented. C1 [Bowman, Kim D.; Faulkner, Lynn] ARS, US Hort Res Lab, USDA, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. [Kesinger, Mike] Florida Dept Agr & Consumer Serv, DPI, Winter Haven, FL 33881 USA. RP Bowman, KD (reprint author), ARS, US Hort Res Lab, USDA, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM kim.bowman@ars.usda.gov FU Florida Citrus Production Research Advisory Council; Florida Citrus Research and Development Foundation FX This research was supported in part by grants from the Florida Citrus Production Research Advisory Council and Florida Citrus Research and Development Foundation. NR 32 TC 0 Z9 0 U1 12 U2 12 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD OCT PY 2016 VL 51 IS 10 BP 1208 EP 1214 DI 10.21273/HORTSC10970-16 PG 7 WC Horticulture SC Agriculture GA EC5NZ UT WOS:000388184100001 ER PT J AU Stover, E Shatters, RG Gruber, B Kumar, P Moore, GA AF Stover, Ed Shatters, Robert G., Jr. Gruber, Barrett Kumar, Prem Moore, Gloria A. TI Influence of Photoperiod Duration and Phloem Disruption through Scoring on Growth, Disease Symptoms, and Bacterial Titer in Citrus Graft Inoculated with Candidatus Liberibacter asiaticus SO HORTSCIENCE LA English DT Article DE citrus greening; disease resistance; HLB; huanglongbing; disease tolerance; stress interaction; SPAD ID GREENING-DISEASE; ROUGH LEMON; INFECTION; GENOTYPES; THAILAND; FLORIDA AB Plants inoculated with the huanglongbing (HLB)-associated bacterium, Candidatus Liberibacter asiaticus (CLas) typically must be monitored for 8-10 months to identify differences in susceptibility between genotypes. Continuous light is reported to accelerate development of HLB symptoms and field observations suggest that trees girdled by tags or tree ties showed greater symptoms. Therefore, an experiment was conducted assessing HLB susceptibility as influenced by light/dark periods of 12 hours: 12 hours and 24 hours: 0 hours, in combination with scoring tree trunks to disrupt phloem. Sixty trees of each of three citrus genotypes ('Kuharske', previously shown to be HLB resistant; rough lemon, previously shown to be HLB tolerant; and 'Valencia', highly HLB susceptible) were bud grafted using two CLas-infected buds (rough lemon and citron) per tree on 26Mar. 2012, and were placed in controlled growth rooms (one 12 hour light: 12 hour dark and one constant light) on 4 June 2012. Ten trees of each genotype in each growth room were scored 10 cm above the soil (cutting through the bark but not the wood) with a knife on 18 July 2012 and the scoring was repeated at the same scoring wounds on 30 Aug. 2012. Trees were removed from growth rooms on 12 Dec. 2012 and subsequently maintained in a greenhouse. At two to three month intervals between June 2012 and May 2013, HLB symptoms and stem diameter at 5 cm above the soil were assessed, and three leaves per tree were collected for quantitative polymerase chain reaction (qPCR) determination of CLas titer. Six months after inoculation and 3 months following imposition of treatments, the 'Valencia' scored in the 12 hour light: 12 hour dark regime, the 'Valencia' non scored trees in 24 hours of light and the 'Kuharske' scored trees in 24 hours of light displayed higher CLas titers than most other trees. After an additional two months, both scored and non-scored trees of all three genotypes in 24 hours of light had significantly elevated CLas titers compared with trees in 12 hour light: 12 hour dark regime, but within most treatments all three genotypes had titers which were not statistically different from each other. Growth of 'Kuharske' and rough lemon was enhanced; whereas 'Valencia' growth was reduced when graft-inoculated plants were maintained in continuous light. Scoring enhanced early CLas development in 'Kuharske' when combined with continuous light, had no effect in rough lemon, and showed inconsistent effects in 'Valencia'. Although continuous lighting enhanced disease progression, it did not reveal differences in HLB susceptibility. C1 [Stover, Ed; Shatters, Robert G., Jr.] ARS, US Hort Res Lab, USDA, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. [Gruber, Barrett; Kumar, Prem] Univ Florida, Indian River Res & Educ Ctr, 2199 South Rock Rd, Ft Pierce, FL 34945 USA. [Moore, Gloria A.] Univ Florida, Dept Hort Sci, 1111 Fifield Hall,Box 110690, Gainesville, FL 32611 USA. RP Stover, E (reprint author), ARS, US Hort Res Lab, USDA, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM ed.stover@ars.usda.gov FU USDA/APHIS FX This project was funded in part through USDA/APHIS. NR 25 TC 0 Z9 0 U1 6 U2 6 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD OCT PY 2016 VL 51 IS 10 BP 1215 EP 1219 DI 10.21273/HORTSCI11084-16 PG 5 WC Horticulture SC Agriculture GA EC5NZ UT WOS:000388184100002 ER PT J AU Reeve, AL Skinkis, PA Vance, AJ Lee, J Tarara, JM AF Reeve, Alison L. Skinkis, Patricia A. Vance, Amanda J. Lee, Jungmin Tarara, Julie M. TI Vineyard Floor Management Influences 'Pinot noir' Vine Growth and Productivity More than Cluster Thinning SO HORTSCIENCE LA English DT Article DE Vitis vinifera; nitrogen; solar radiation environment; cover crop; photoassimilation; cool climate viticulture ID VITIS-VINIFERA L.; REGULATED DEFICIT-IRRIGATION; SAUVIGNON BLANC GRAPEVINES; LEAF GAS-EXCHANGE; FRUIT COMPOSITION; CROP LOAD; COVER CROP; CABERNET-SAUVIGNON; WINE QUALITY; WATER-USE AB Vigor and crop level management are important practices for premium wine grape production. The implications of crop thinning 'Pinot noir'(Vitis vinifera L.) vines of varying vigor were investigated in the Willamette Valley of Oregon in 2011 to 2013 to better understand the relationship between canopy size and yield within the framework of a cool-climate, premium production wine grape vineyard. To manipulate vigor, a competitive grass cover crop (Festuca rubra L.) was grown in both (Grass), alternating (Alternate), or neither side of the flanking alleyways (Tilled). Vines within each vineyard floor treatment had two crop levels applied, including cluster thinning to one cluster per shoot (Half Crop) or no crop thinning (Full Crop). Grass treatment had reduced leaf area and leaf nitrogen (N) concentrations during all years compared with Tilled treatments. Leaf photosynthesis was also lower in Grass treatments despite more light in the canopy interior. Grass treatments had lower yield than Tilled treatments in 2 of 3 years and lower yeast assimilable nitrogen (YAN) concentrations in fruit every year. There was limited impact of floor treatments on total soluble solids (TSS) and pH. Reduced yields through cluster thinning had limited impact on vegetative growth but increased TSS and pH, in 2 of 3 years. There were few floor management by crop level interactions in any year. Grass effectively reduced vegetative growth to moderate vigor levels with cane weights between 20 and 40 g. Using a competitive grass cover crop may be an effective strategy to reduce excessive vine growth and require less labor in canopy management and crop thinning without compromising basic fruit ripeness, although YAN levels need to be monitored. C1 [Reeve, Alison L.; Skinkis, Patricia A.; Vance, Amanda J.] Oregon State Univ, Dept Hort, 4017 ALS Bldg, Corvallis, OR 97331 USA. [Lee, Jungmin] ARS, USDA, Hort Crops Res Unit Corvallis OR Worksite, 29603 U 1 Lane, Parma, ID 83660 USA. [Tarara, Julie M.] Ste Michelle Wine Estates, 660 Frontier Rd, Prosser, WA 99350 USA. RP Skinkis, PA (reprint author), Oregon State Univ, Dept Hort, 4017 ALS Bldg, Corvallis, OR 97331 USA. EM patricia.skinkis@oregonstate.edu FU Northwest Center for Small Fruits Research (NCSFR); USDA-ARS CRIS (Current Research Information System) project [2072-21000-047-00D]; Oregon Wine Board FX This research project was led by PI (Skinkis) and two sequential graduate students (Vance 2011) and Reeve (2012 onward). Project co-PIs (Tarara and Lee) provided expertise in methodology and data interpretation and partial funding. This project was supported, in part, by funds from the Northwest Center for Small Fruits Research (NCSFR), USDA-ARS CRIS (Current Research Information System) project number 2072-21000-047-00D, and the Oregon Wine Board. The authors greatly appreciate Rob Schultz, Bill Stoller, and others of Stoller Family Estate Vineyards (Dayton, OR) for their cooperation in conducting this research on-site. NR 77 TC 0 Z9 0 U1 8 U2 8 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD OCT PY 2016 VL 51 IS 10 BP 1233 EP 1244 DI 10.21273/HORTSC10998-16 PG 12 WC Horticulture SC Agriculture GA EC5NZ UT WOS:000388184100005 ER PT J AU Tong, CBS Chang, HY Boldt, JK Ma, YZB DeEll, JR Moran, RE Bourgeois, G Plouffe, D AF Tong, Cindy B. S. Chang, Hsueh-Yuan Boldt, Jennifer K. Ma, Yizhou B. DeEll, Jennifer R. Moran, Renae E. Bourgeois, Gaetan Plouffe, Dominique TI Diffuse Flesh Browning in 'Honeycrisp' Apple Fruit is Associated with Low Temperatures during Fruit Growth SO HORTSCIENCE LA English DT Article DE Malus xdomestica; disorder; storage; sugars; weather ID STORAGE DISORDERS; MINERAL-COMPOSITION; HARVEST MATURITY; SOFT SCALD; BITTER PIT; QUALITY; SPRAYS; ANTIOXIDANT; PHOSPHORUS; ATMOSPHERE AB Multiple types of flesh browning can occur as storage disorders in 'Honeycrisp' apple (Malus x domestica Borkh.) fruit. Predicting its occurrence is hindered by differing definitions of the types of browning, incomplete understanding of their etiologies, and difficulty in assessing harvest maturity of 'Honeycrisp' fruit. In 2013, of 'Honeycrisp' fruit grown, harvested over multiple weeks, and stored in Maine, Minnesota, Ontario, and Quebec, only the Quebec fruit developed diffuse flesh browning. A detailed comparison showed that the Quebec fruit differed in size, but not in other quality attributes, from fruit of the other locations. The Quebec fruit experienced lower temperatures during active fruit growth and were increasing in cell size up to harvest. Analyses of climate data from 2009 to 2015 indicated that accumulated growing degree-days (GDD) 50-60 day after full bloom (DAFB) could account for 31% of the variation in diffuse flesh browning, and seasonal GDD <500 are associated with a greater likelihood of injury. Fruit that exhibited diffuse flesh browning had higher magnesium and lower fructose levels than unaffected fruit. As these measurements were made after browning was assessed, the timing of the onset of these characteristics in relation to browning cannot be determined. C1 [Tong, Cindy B. S.; Chang, Hsueh-Yuan; Boldt, Jennifer K.; Ma, Yizhou B.] Univ Minnesota, Dept Hort Sci, 1970 Folwell Ave, St Paul, MN 55108 USA. [DeEll, Jennifer R.] Ontario Minist Agr Food & Rural Affairs, Simcoe, ON N3Y 4N5, Canada. [Moran, Renae E.] Univ Maine, Dept Plant Soil & Environm Sci, POB 179, Monmouth, ME 04259 USA. [Bourgeois, Gaetan; Plouffe, Dominique] Agr & Agri Food Canada, Hort Res & Dev Ctr, 430 Blvd Gouin, St Jean, PQ J3B 3E6, Canada. [Boldt, Jennifer K.] ARS, USDA, Greenhouse Prod Res Grp, 2801 W Bancroft St,Mail Stop 604, Toledo, OH 43606 USA. RP Tong, CBS (reprint author), Univ Minnesota, Dept Hort Sci, 1970 Folwell Ave, St Paul, MN 55108 USA. EM c-tong@umn.edu FU Minnesota Agricultural Experiment Station for MN [21-065]; University of Minnesota Undergraduate Research Opportunity Program; Maine Agricultural and Forest Experiment Station Project [ME031404] FX This project received funding from the Minnesota Agricultural Experiment Station for MN 21-065, University of Minnesota Undergraduate Research Opportunity Program, and Maine Agricultural and Forest Experiment Station Project ME031404. NR 33 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD OCT PY 2016 VL 51 IS 10 BP 1256 EP 1264 DI 10.21273/HORTSCI11179-16 PG 9 WC Horticulture SC Agriculture GA EC5NZ UT WOS:000388184100008 ER PT J AU Shock, CC Feibert, EBG Rivera, A Saunders, LD Shaw, N Kilkenny, FF AF Shock, Clinton C. Feibert, Erik B. G. Rivera, Alicia Saunders, Lamont D. Shaw, Nancy Kilkenny, Francis F. TI Irrigation Requirements for Seed Production of Five Lomatium Species in a Semiarid Environment SO HORTSCIENCE LA English DT Article DE subsurface drip irrigation; medicinal plant; rangeland restoration ID FERTILIZATION; ESTABLISHMENT; DISSECTUM; GROWTH; OREGON AB Seeds of native plants are needed for rangeland restoration in the Intermountain West. Many of these plants are rarely cultivated and relatively little is known about the cultural practices required for their seed production. Irrigation trials were conducted for five perennial Lomatium species over multiple years. Lomatium species grown at the Oregon State University Malheur Experiment Station, Ontario, OR received 0, 100, or 200 mm of irrigation per year. Seed yield responses to irrigation were evaluated by linear and quadratic regression. In general, seed yields from the three species grown for 10 years responded linearly or quadratically to irrigation. To improve the accuracy of estimated irrigation water requirements, regressions were also run on seed yield responses to irrigation plus precipitation during the previous spring; spring and winter; and spring, winter, and fall. Over multiple years, Lomatium dissectum (Nutt.) Mathias & Constance and L. triternatum (Pursh) J.M. Coult. & Rose seed yields were best estimated by a quadratic response to irrigation plus spring precipitation with highest yields at 243 and 255 mm, respectively. Lomatium grayi (J.M. Coult. & Rose) J.M. Coult. & Rose seed yields were best estimated by a quadratic response to irrigation plus precipitation during the fall, winter, and spring with highest yields at 358 mm. Two of the Lomatium species were grown for the last 6 years. The seed yields of L. nudicaule (Pursh) J.M. Coult. & Rose did not respond to irrigation. Seed yields of Lomatium suksdorfii (S. Watson) J.M. Coult. & Rose responded linearly to irrigation in 2015. C1 [Shock, Clinton C.; Feibert, Erik B. G.; Rivera, Alicia; Saunders, Lamont D.] Oregon State Univ, Malheur Expt Stn, 595 Onion Ave, Ontario, OR 97914 USA. [Shaw, Nancy; Kilkenny, Francis F.] US Forest Serv, Rocky Mt Res Stn, 322 E Front St,Suite 401, Boise, ID 83702 USA. RP Shock, CC (reprint author), Oregon State Univ, Malheur Expt Stn, 595 Onion Ave, Ontario, OR 97914 USA. EM clinton.shock@oregonstate.edu FU USDA Forest Service; Rocky Mountain Research Station's Great Basin Native Plant Project; USDI Bureau of Land Management; Oregon State University; Malheur County Education Service District; USDA National Institute of Food and Agriculture [2015-31100-06041, 2015-31200-06041] FX This project was partially funded by the USDA Forest Service, Rocky Mountain Research Station's Great Basin Native Plant Project, USDI Bureau of Land Management, Oregon State University, Malheur County Education Service District, and by formula grants 2015-31100-06041 and 2015-31200-06041 from the USDA National Institute of Food and Agriculture. NR 21 TC 0 Z9 0 U1 7 U2 7 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD OCT PY 2016 VL 51 IS 10 BP 1270 EP 1277 DI 10.21273/HORTSCI11150-16 PG 8 WC Horticulture SC Agriculture GA EC5NZ UT WOS:000388184100010 ER PT J AU Hugie, KL Watkins, E AF Hugie, Kari L. Watkins, Eric TI Performance of Low-input Turfgrass Species as Affected by Mowing and Nitrogen Fertilization in Minnesota SO HORTSCIENCE LA English DT Article DE hard fescue; prairie junegrass; tufted hairgrass; colonial bentgrass; nitrogen fertilizer; mowing ID JUNEGRASS GERMPLASM ACCESSIONS; MANAGEMENT; QUALITY; GRASSES; RESPONSES AB In Minnesota, most lawns and higher cut turfgrass areas consist primarily of species such as kentucky bluegrass (Poa pratensis L.) and perennial ryegrass (Lolium perenne L.) that require significant management inputs such as frequent mowing and nitrogen fertility. Several studies have shown that other species have the potential to be used more widely on home lawns in Minnesota; however, little is known about the management requirements of these species. In this study, we evaluated the performance of several alternative grass species under varying mowing and nitrogen fertility regimes at two sites in Minnesota in 2010 and 2011. Hard fescue [Festuca trachyphylla (Hackel) Krajina] showed the most consistent performance across management regimes, seasons, and locations. Colonial bentgrass (Agrostis tenuis Sibth.) showed good spring and fall turf quality, but suffered from excess thatch development and disease incidence. 'Barkoel' prairie junegrass [Koeleria macrantha (Ledeb.) Schult] maintained acceptable turf cover throughout the trial, whereas unimproved native prairie junegrass populations did poorly regardless of management level. Tufted hairgrass [Deschampsia cespitosa (L.) P. Beauv.] did not perform consistently in the trial due to summer stress. Our results show that hard fescue, colonial bentgrass, and 'Barkoel' prairie junegrass performed well regardless of mowing height or fertility treatment and could be used to a greater degree as low-input turfgrasses in Minnesota. C1 [Hugie, Kari L.] ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, 2611 West Lucas St, Florence, SC 29501 USA. [Watkins, Eric] Univ Minnesota, Dept Hort Sci, 1970 Folwell Ave, St Paul, MN 55108 USA. RP Watkins, E (reprint author), Univ Minnesota, Dept Hort Sci, 1970 Folwell Ave, St Paul, MN 55108 USA. EM ewatkins@umn.edu FU Cooperative State Research, Education, and Extension Service, U.S. Department of Agriculture [2009-34381-20044] FX This material is based on research supported by the Cooperative State Research, Education, and Extension Service, U.S. Department of Agriculture, under agreement no. 2009-34381-20044. NR 31 TC 0 Z9 0 U1 4 U2 4 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD OCT PY 2016 VL 51 IS 10 BP 1278 EP 1286 DI 10.21273/HORTSCI11007-16 PG 9 WC Horticulture SC Agriculture GA EC5NZ UT WOS:000388184100011 ER PT J AU Miao, H Zhang, SP Wang, M Wang, Y Weng, YQ Gu, XF AF Miao, Han Zhang, Shengping Wang, Min Wang, Ye Weng, Yiqun Gu, Xingfang TI Fine Mapping of Virescent Leaf Gene v-1 in Cucumber (Cucumis sativus L.) SO INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES LA English DT Article DE cucumber; virescent leaf; chloroplast development; map-based cloning ID BURROWS-WHEELER TRANSFORM; ARABIDOPSIS-THALIANA; CHLOROPLAST DEVELOPMENT; CHLOROPHYLL BIOSYNTHESIS; MUTANT REVEALS; NUCLEAR GENE; RICE; PROTEIN; GENOME; WHEAT AB Leaf color mutants are common in higher plants that can be used as markers in crop breeding or as an important tool in understanding regulatory mechanisms in chlorophyll biosynthesis and chloroplast development. In virescent leaf mutants, young leaves are yellow in color, which gradually return to normal green when the seedlings grow large. In the present study, we conducted phenotypic characterization and genetic mapping of the cucumber virescent leaf mutant 9110Gt conferred by the v-1 locus. Total chlorophyll and carotenoid content in 9110Gt was reduced by 44% and 21%, respectively, as compared with its wild type parental line 9110G. Electron microscopic investigation revealed fewer chloroplasts per cell and thylakoids per chloroplast in 9110Gt than in 9110G. Fine genetic mapping allowed for the assignment of the v-1 locus to a 50.4 kb genomic DNA region in chromosome 6 with two flanking markers that were 0.14 and 0.16 cM away from v-1, respectively. Multiple lines of evidence supported CsaCNGCs as the only candidate gene for the v-1 locus, which encoded a cyclic-nucleotide-gated ion channel protein. A single nucleotide change in the promoter region of v-1 seemed to be associated with the virescent color change in 9110Gt. Real-time PCR revealed significantly lower expression of CsaCNGCs in the true leaves of 9110Gt than in 9110G. This was the first report that connected the CsaCNGCs gene to virescent leaf color change, which provided a useful tool to establish linkages among virescent leaf color change, chloroplast development, chlorophyll biosynthesis, and the functions of the CsaCNGCs gene. C1 [Miao, Han; Zhang, Shengping; Wang, Min; Wang, Ye; Gu, Xingfang] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China. [Miao, Han; Weng, Yiqun] Univ Wisconsin, Dept Hort, USDA ARS Vegetable Crops Res Unit, Madison, WI 53706 USA. RP Gu, XF (reprint author), Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China.; Weng, YQ (reprint author), Univ Wisconsin, Dept Hort, USDA ARS Vegetable Crops Res Unit, Madison, WI 53706 USA. EM miaohan@caas.cn; zhangshengping@caas.cn; w.jsun@163.com; wangye@caas.cn; yiqun.weng@ars.usda.gov; guxingfang@mail.caas.cn FU China Scholarship Council; Natural Science Foundation of China [31101549]; China Agriculture Research System [CARS-25]; Science and Technology Innovation Program of the Chinese Academy of Agricultural Science (CAAS-ASTIP-IVFCAAS); Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, China FX The authors thank Caiqing Xu and Kristin Haider for technical assistance and Graham Collins (Roseworthy Campus of the University of Adelaide in South Australia) for revision of the manuscript. HM's work in YW's laboratory was partially funded by the China Scholarship Council. This research was supported by the Natural Science Foundation of China (31101549), China Agriculture Research System (CARS-25), Science and Technology Innovation Program of the Chinese Academy of Agricultural Science (CAAS-ASTIP-IVFCAAS), and the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, China. NR 66 TC 0 Z9 0 U1 5 U2 5 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1422-0067 J9 INT J MOL SCI JI Int. J. Mol. Sci. PD OCT PY 2016 VL 17 IS 10 AR 1602 DI 10.3390/ijms17101602 PG 12 WC Biochemistry & Molecular Biology; Chemistry, Multidisciplinary SC Biochemistry & Molecular Biology; Chemistry GA EC0DO UT WOS:000387768300182 ER PT J AU Rinella, MJ Espeland, EK Moffatt, BJ AF Rinella, Matthew J. Espeland, Erin K. Moffatt, Bruce J. TI Studying long-term, large-scale grassland restoration outcomes to improve seeding methods and reveal knowledge gaps SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE adaptive management; Agropvron cristatum; Bromus tectorum; compositional data analysis; crested wheatgrass; Great Plains; rangeland; reclamation; seed rate; shrubs ID ADAPTIVE-MANAGEMENT; PERENNIAL GRASSES; PLANT-COMMUNITIES; ECOLOGICAL RESTORATION; POPULATION-DYNAMICS; MODELING APPROACH; COMPETITION; ESTABLISHMENT; RANGELAND; FERTILIZATION AB 1. Studies are increasingly investigating effects of large-scale management activities on grassland restoration outcomes. These studies are providing useful comparisons among currently used management strategies, but not the novel strategies needed to rapidly improve restoration efforts. Here we illustrate how managing restoration projects adaptively can allow promising management innovations to be identified and tested. 2. We studied 327 Great Plains fields seeded after coal mining. We modelled plant responses to management strategies to identify the most effective previously used strategies for constraining weeds and establishing desired plants. Then, we used the model to predict responses to new strategies our analysis identified as potentially more effective. 3. Where established, the weed crested wheatgrass (Agropyron cristatum L.) increased through time, indicating a need to manage establishment of this grass. Seeding particular grasses reduced annual weed cover, and because these grasses appeared to become similarly abundant whether sown at low or high rates, low rates could likely be safely used to reduce seeding costs. More importantly, lower than average grass seed rates increased cover of shrubs, the plants most difficult to restore to many grassland ecosystems. After identifying grass seed rates as a driver, we formulated model predictions for rates below the range managers typically use. These predictions require testing but indicated atypically low grass seed rates would further increase shrubs without hindering long-term grass stand development. 4. Synthesis and applications. Designing management around empirically based predictions is a logical next step towards improving ecological restoration efforts. Our predictions are that reducing grass seed rates to atypically low levels will boost shrubs without compromising grasses. Because these predictions derive from the fitted model, they represent quantitative hypotheses based on current understanding of the system. Generating data needed to test and update these hypotheses will require monitoring responses to shifts in management, specifically shifts to lower grass seed rates. A paucity of data for confronting hypotheses has been a major sticking point hindering adaptive management of most natural resources, but this need not he the case with degraded grasslands, because ongoing restoration efforts around the globe are providing continuous opportunities to monitor and manage processes regulating grassland restoration outcomes. C1 [Rinella, Matthew J.] USDA ARS, Rangeland Ecol, Ft Keogh Livestock & Range Res Lab, 243 Ft Keogh Rd, Miles City, MT 59301 USA. [Espeland, Erin K.] USDA ARS, Pest Management Unit, Northern Plains Agr Res Lab, Sidney, MT USA. [Moffatt, Bruce J.] Oregon State Univ, Dept Soil Sci, Corvallis, OR 97331 USA. RP Rinella, MJ (reprint author), USDA ARS, Rangeland Ecol, Ft Keogh Livestock & Range Res Lab, 243 Ft Keogh Rd, Miles City, MT 59301 USA. EM Matt.Rinella@ars.usda.gov NR 66 TC 0 Z9 0 U1 15 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD OCT PY 2016 VL 53 IS 5 BP 1565 EP 1574 DI 10.1111/1365-2664.12722 PG 10 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA EC0FE UT WOS:000387773000028 ER PT J AU Livingston, AC Varner, JM Jules, ES Kane, JM Arguello, LA AF Livingston, Amy C. Varner, J. Morgan Jules, Erik S. Kane, Jeffrey M. Arguello, Leonel A. TI Prescribed fire and conifer removal promote positive understorey vegetation responses in oak woodlands SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE conifer encroachment; ecological restoration; non-metric multidimensional scaling; non-native species; oak woodlands; Oregon white oak; prescribed fire; Pseudotsuga menziesii; Quercus garryana; understorey vegetation ID WOODY PLANT ENCROACHMENT; PACIFIC-NORTHWEST; SPECIES RICHNESS; MESIC GRASSLAND; WESTERN OREGON; FORT-LEWIS; SAVANNA; WASHINGTON; PRAIRIES; FOREST AB 1. Fire-prone woodlands and savannas world-wide face management challenges resulting from fire exclusion and subsequent encroachment of fire sensitivetrees. In the Pacific Northwest (USA), Quercus garryana oak woodlands and savannas are threatened by encroachment from the native conifer Pseudotsuga menziesii in the absence of fire. 2. In the Bald Hills of Redwood National Park (California, USA), prescribed fire and conifer removal have been used to restore encroached woodlands. We examined the effects of encroachment and restoration on understorey vegetation, comparing four treatments: prescribed fire, prescribed fire and conifer removal, conifer removal, and encroached (control). 3. Treatments including prescribed fire had the greatest native species richness. These two treatments also had the greatest non-native species richness, at both the site level and the treatment level. Woodlands treated with conifer removal and no prescribed lire were intermediate in species richness and diversity compared to burned treatments and encroached woodlands. Encroached woodlands had diminished richness and diversity compared to all restoration treatments. Non-metric multidimensional scaling (NMS) ordination demonstrated that conifer basal area, conifer litter and fine wood were associated with low species richness and diversity and that elevation and thatch were associated with higher species richness and diversity. Indicator species analysis identified that most native species and non-native species were associated with burned woodlands that were never encroached. 4. Synthesis and applications. Our results suggest that both prescribed fire and conifer removal have benefits for understorey plant communities, increasing species richness, diversity and cover in oak woodlands and shifting understorey communities from forest-associated species to more woodland-associated species. Restoration of remnant Quercus garryana oak woodlands is complicated by the persistence and abundance of non-native herbaceous plants. C1 [Livingston, Amy C.; Varner, J. Morgan; Kane, Jeffrey M.] Humboldt State Univ, Dept Forestry & Wildland Resources, Wildland Fire Lab, Arcata, CA 95521 USA. [Varner, J. Morgan] US Forest Serv, USDA, Pacific Northwest Res Stn, Pacific Wildland Fire Sci Lab, Seattle, WA 98103 USA. [Jules, Erik S.] Humboldt State Univ, Dept Biol Sci, Arcata, CA 95521 USA. [Arguello, Leonel A.] Redwood Natl Park, Orick, CA 95555 USA. RP Livingston, AC (reprint author), Trinity Cty Resource Conservat Dist, POB 1450, Weaverville, CA 96093 USA. EM acl15@humboldt.edu FU USDA McIntire-Stennis Program; University of California Agriculture and Natural Resources Program FX Funding was provided by the USDA McIntire-Stennis Program and the University of California Agriculture and Natural Resources Program. Advice on statistical analysis was provided by R. Van Kirk. Discussions with E. Engber, S. Samuels, J. McClelland, E. Asarian and J. Deshais improved this work. K. Eldridge, P. Nguyen, A. Lottes, N. Hancock, E. Nichols, A. Shackelford, K. Belief, A. Geritz, L. Lascheck and M. Schriver assisted with data collection. The paper benefitted from thoughtful comments and criticisms provided by anonymous reviewers. NR 60 TC 0 Z9 0 U1 19 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD OCT PY 2016 VL 53 IS 5 BP 1604 EP 1612 DI 10.1111/1365-2664.12703 PG 9 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA EC0FE UT WOS:000387773000032 ER PT J AU Cameron, RG Chau, HK Manthey, JA AF Cameron, Randall G. Chau, Hoa K. Manthey, John A. TI Continuous process for enhanced release and recovery of pectic hydrocolloids and phenolics from citrus biomass SO JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY LA English DT Article DE citrus; pectin; phenolics; steam-explosion; agricultural waste valorization ID SUPERCRITICAL-FLUID EXTRACTION; SIMULTANEOUS SACCHARIFICATION; ORANGE PEEL; WASTE; ACID; FERMENTATION; PRETREATMENT; LIMONOIDS; ETHANOL AB BACKGROUND: During the 2012/2013 harvest season the Florida citrus juice industry produced 0.55x10(6) metric tons (MT) of dried citrus pellets from citrus processing waste (CPW). The citrus pellets were marketed as a low value animal feed which typically sold for $0.22 per kg or less. Yet, this biomass also contained nearly 110 000MT of pectic hydrocolloids and 25 000MT of flavonoids and related phenolics as potential value-added co-products. A continuous process was explored to release and recover these co-products in a manner that enables their desired functionalities. RESULTS: Injecting steam into the flow of citrus peel through a pipe at 150 degrees C released pectic hydrocolloids and phenolic compounds, as well as several other classes of secondary metabolites from the waste biomass. The resulting steam-exploded peel was washed with water and pectic hydrocolloids and several major classes of phenolic compounds were recovered in the wash water. Over two seasons an average of 72% of the pectic hydrocolloids were recovered. In addition, 41.1% of the polymethoxylated flavones, 11.4% of the flavanone glycosides, 85.2% of the limonoids and nearly 100% of hydroxycinnamates were recovered with the water wash. CONCLUSION: The continuous steam treatment of citrus processing waste provides an enhanced, environmentally friendly method for release and recovery of valuable pectic hydrocolloids and phenolic compounds. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. C1 [Cameron, Randall G.; Manthey, John A.] ARS, USDA, US Hort Res Lab, Citrus & Other Subtrop Prod Res Unit, Ft Pierce, FL 34952 USA. [Chau, Hoa K.] ARS, USDA, Eastern Reg Res Ctr, Dairy & Funct Foods Res Unit, Wyndmoor, PA 19038 USA. RP Cameron, RG (reprint author), ARS, USDA, US Hort Res Lab, Citrus & Other Subtrop Prod Res Unit, Ft Pierce, FL 34952 USA. EM Randall.cameron@ars.usda.gov NR 42 TC 0 Z9 0 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0268-2575 EI 1097-4660 J9 J CHEM TECHNOL BIOT JI J. Chem. Technol. Biotechnol. PD OCT PY 2016 VL 91 IS 10 BP 2597 EP 2606 DI 10.1002/jctb.4854 PG 10 WC Biotechnology & Applied Microbiology; Chemistry, Multidisciplinary; Engineering, Environmental; Engineering, Chemical SC Biotechnology & Applied Microbiology; Chemistry; Engineering GA EC7BX UT WOS:000388291900006 ER PT J AU Castro, P Lewers, KS AF Castro, P. Lewers, K. S. TI Identification of quantitative trait loci (QTL) for fruit-quality traits and number of weeks of flowering in the cultivated strawberry SO MOLECULAR BREEDING LA English DT Article DE Fragaria x ananassa; Flowering habit; Fruit-quality traits; QTL analysis; Molecular markers ID OCTOPLOID FRAGARIA-VIRGINIANA; ASCORBIC-ACID CONTENT; X-ANANASSA DUCH; DIPLOID FRAGARIA; DAY-NEUTRALITY; LINKAGE MAPS; XANANASSA DUCH.; SEX-CHROMOSOME; HUMAN HEALTH; INHERITANCE AB Fruit quality and repeat flowering are two major foci of several strawberry breeding programs. The identification of quantitative trait loci (QTL) and molecular markers linked to these traits could improve breeding efficiency. In this work, an F1 population derived from the cross 'Delmarvel' 9 'Selva' was used to develop a genetic linkage map for QTL analyses of fruit-quality traits and number of weeks of flowering. Some QTL for fruit-quality traits were identified on the same homoeologous groups found in previous studies, supporting trait association in multiple genetic backgrounds and utility in multiple breeding programs. None of the QTL for soluble solids colocated with a QTL for titratable acids, and, although the total soluble solid contents were significantly and positively correlated with titratable acids, the correlation coefficient value of 0.2452 and independence of QTL indicate that selection for high soluble solids can be practiced independently of selection for low acidity. One genomic region associated with the total number of weeks of flowering was identified quantitatively on LG IV-S-1. The most significant marker, FxaACAO2I8C-145S, explained 43.3 % of the phenotypic variation. The repeat-flowering trait, scored qualitatively, mapped to the same region as the QTL. Dominance of the repeat-flowering allele was demonstrated by the determination that the repeat-flowering parent was heterozygous. This genomic region appears to be the same region identified in multiple mapping populations and testing environments. Markers linked in multiple populations and testing environments to fruit-quality traits and repeat flowering should be tested widely for use in marker-assisted breeding. C1 [Castro, P.; Lewers, K. S.] ARS, USDA, Genet Improvement Fruits & Vegetables Lab, Bldg 010A BARC West,10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Lewers, KS (reprint author), ARS, USDA, Genet Improvement Fruits & Vegetables Lab, Bldg 010A BARC West,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Kim.Lewers@ars.usda.gov FU USDA-ARS [8042-21220-254-00] FX This project was funded by USDA-ARS Project 8042-21220-254-00. The authors wish to thank Drs. Stan Hokanson and Sarah Nourse who generated the population before moving on to other research opportunities, Dr. Show Wang who collected the anthocyanin, phenolics and ORAC data before retiring and passing on, and the many former USDA-ARS technicians and undergraduate students who worked on this population at some point while associated with the project. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture or any of the other agencies involved in this research. NR 69 TC 0 Z9 0 U1 6 U2 6 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD OCT PY 2016 VL 36 IS 10 AR 138 DI 10.1007/s11032-016-0559-7 PG 19 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA EC5YA UT WOS:000388212000003 ER PT J AU Pankratov, I McQuinn, R Schwartz, J Bar, E Fei, ZJ Lewinsohn, E Zamir, D Giovannoni, JJ Hirschberg, J AF Pankratov, Ilya McQuinn, Ryan Schwartz, Jochanan Bar, Einat Fei, Zhangjun Lewinsohn, Efraim Zamir, Dani Giovannoni, James J. Hirschberg, Joseph TI Fruit carotenoid-deficient mutants in tomato reveal a function of the plastidial isopentenyl diphosphate isomerase (IDI1) in carotenoid biosynthesis SO PLANT JOURNAL LA English DT Article DE isoprenoid biosynthesis; carotenoids; fruit development; Solanum lycopersicum; plastid; de-etiolation ID METHYLERYTHRITOL PHOSPHATE-PATHWAY; ISOPRENOID BIOSYNTHESIS; ARABIDOPSIS-THALIANA; PHYTOENE SYNTHASE; BACILLUS-SUBTILIS; ESCHERICHIA-COLI; MEVALONATE PATHWAY; HIGHER-PLANTS; SWEET BASIL; GENE AB Isoprenoids consist of a large class of compounds that are present in all living organisms. They are derived from the 5C building blocks isopentenyl diphosphate (IDP) and its isomer dimethylallyl diphosphate (DMADP). In plants, IDP is synthesized in the cytoplasm from mevalonic acid via the MVA pathway, and in plastids from 2-C-methyl-D-erythritol-4-phosphate through the MEP pathway. The enzyme IDP isomerase (IDI) catalyzes the interconversion between IDP and DMADP. Most plants contain two IDI enzymes, the functions of which are characteristically compartmentalized in the cells. Carotenoids are isoprenoids that play essential roles in photosynthesis and provide colors to flowers and fruits. They are synthesized in the plastids via the MEP pathway. Fruits of Solanum lycopersicum (tomato) accumulate high levels of the red carotene lycopene. We have identified mutations in tomato that reduce overall carotenoid accumulation in fruits. Four alleles of a locus named FRUIT CAROTENOID DEFICIENT 1 (fcd1) were characterized. Map-based cloning of fcd1 indicated that this gene encodes the plastidial enzyme IDI1. Lack of IDI1 reduced the concentration of carotenoids in fruits, flowers and cotyledons, but not in mature leaves. These results indicate that the plastidial IDI plays an important function in carotenoid biosynthesis, thus highlighting its role in optimizing the ratio between IDP and DMADP as precursors for different downstream isoprenoid pathways. C1 [Pankratov, Ilya; Schwartz, Jochanan; Hirschberg, Joseph] Hebrew Univ Jerusalem, Alexander Silberman Inst Life Sci, Dept Genet, IL-91904 Jerusalem, Israel. [McQuinn, Ryan; Fei, Zhangjun; Giovannoni, James J.] Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA. [McQuinn, Ryan; Giovannoni, James J.] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Bar, Einat; Lewinsohn, Efraim] Agr Res Org, Inst Field & Vegetable Crops, Newe Yaar Res Ctr, POB 1021, Ramat Yishay, Israel. [Zamir, Dani] Hebrew Univ Jerusalem, Robert H Smith Inst Plant Sci & Genet, Fac Agr, IL-91904 Jerusalem, Israel. RP Hirschberg, J (reprint author), Hebrew Univ Jerusalem, Alexander Silberman Inst Life Sci, Dept Genet, IL-91904 Jerusalem, Israel. EM hirschu@mail.huji.ac.il FU Israel Science Foundation [1685/09]; EU-FP7 METAPRO [244348] FX This research was supported by grants 1685/09, from the Israel Science Foundation, and EU-FP7 METAPRO 244348. NR 72 TC 0 Z9 0 U1 14 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0960-7412 EI 1365-313X J9 PLANT J JI Plant J. PD OCT PY 2016 VL 88 IS 1 BP 82 EP 94 DI 10.1111/tpj.13232 PG 13 WC Plant Sciences SC Plant Sciences GA EC9CS UT WOS:000388442100007 PM 27288653 ER PT J AU Schreiner, RP AF Schreiner, R. Paul TI Nutrient Uptake and Distribution in Young Pinot noir Grapevines over Two Seasons SO AMERICAN JOURNAL OF ENOLOGY AND VITICULTURE LA English DT Article DE biomass; micronutrients; nitrogen; nutrient reserves; phosphorus; potassium ID THOMPSON SEEDLESS GRAPEVINES; VITIS-VINIFERA L.; DRY-MATTER; ACCUMULATION; NITROGEN; GROWTH; EFFICIENCY; BERRIES; ROOTS; YIELD AB The seasonal timing of biomass and nutrient distribution among different vine organs was determined over two growing seasons in four-year-old Pinot noir grapevines grown in field microplots. Vines were fertilized in spring, and biomass and nutrient contents of nine separate vine parts were measured at six phenological stages (budbreak, bloom, veraison, harvest, leaf fall, and dormancy) each year. The uptake and distribution of four micronutrients-boron (B), zinc (Zn), Manganese (Mn), and copper (Cu)-was determined for the first time in field-grown grapevines. Whole-vine nitrogen (N) uptake was maximal early in the season, with most N uptake occurring before bloom. Uptake of phosphorus (P) and sulfur (S) was also early compared to other nutrients, with similar quantities of these elements taken up between budbreak and bloom and between bloom and veraison. All other macronutrients-potassium (K), calcium (Ca), and magnesium (Mg)-and micronutrients (B, Zn, Mn, Cu) had peak uptake between bloom and veraison. Remobilization of nutrients from permanent vine structures helped supply early season canopy needs for N, K, and S. More N was remobilized from reserves and this lasted until veraison, supplying similar to 35% of the canopy N between budbreak and veraison. Remobilization of K and S occurred only until bloom and contributed similar to 30% of the canopy increase in these elements up to that time. The small root fraction was the main source of remobilized nutrients for N, K, and S. A net annual gain of nutrients in the permanent vine parts occurred for P, K, Ca, Mg, Zn, and Cu. The quantities of each nutrient required by young Pinot noir grapevines carrying a typical crop yield for the region are presented and discussed. C1 [Schreiner, R. Paul] ARS, USDA, Hort Crops Res Lab, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. RP Schreiner, RP (reprint author), ARS, USDA, Hort Crops Res Lab, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. EM paul.schreiner@ars.usda.gov NR 31 TC 0 Z9 0 U1 7 U2 7 PU AMER SOC ENOLOGY VITICULTURE PI DAVIS PA PO BOX 1855, DAVIS, CA 95617-1855 USA SN 0002-9254 EI 1943-7749 J9 AM J ENOL VITICULT JI Am. J. Enol. Vitic. PD OCT PY 2016 VL 67 IS 4 BP 436 EP 448 DI 10.5344/ajev.2016.16019 PG 13 WC Biotechnology & Applied Microbiology; Food Science & Technology; Horticulture SC Biotechnology & Applied Microbiology; Food Science & Technology; Agriculture GA EC4KF UT WOS:000388097000009 ER PT J AU Tognella, MMP Soares, MLG Cuevas, E Medina, E AF Tognella, M. M. P. Soares, M. L. G. Cuevas, E. Medina, E. TI Heterogeneity of elemental composition and natural abundance of stables isotopes of C and N in soils and leaves of mangroves at their southernmost West Atlantic range SO BRAZILIAN JOURNAL OF BIOLOGY LA English DT Article DE mangroves; soils; leaves; nutrients; stable isotopes; southwestern Atlantic latitudinal limit ID RHIZOPHORA-MANGLE; NUTRIENT RELATIONS; ORGANIC-CARBON; RIVER ESTUARY; SEPETIBA BAY; HEAVY-METALS; PUERTO-RICO; VARIABILITY; FORESTS; FLORIDA AB Mangrove communities were selected in the state of Santa Catarina, Brazil, near their southernmost limit of distribution, to study mineral nutrient relation in soils and plants. Communities included three true mangrove species, Rhizophora mangle, Laguncularia racemosa and Avicennia germinans, and two associated species, the fern Acrostichum danaeifolium, and the grass Spartina densiflora. The sites included communities in the lower Rio Tavares near Florianopolis city, Sonho beach near Palhoca city, and the Santo Antonio lagoon. These sites included a full range of mangroves under humid climate where winter temperatures, instead of salinity, may be the main factor regulating their productive capacity and species composition. Soil salinity was determined by the concentration of soluble Na, and soil C and N were linearly correlated indicating their association in organic matter. Tavares site showed higher specific conductivity, and concentrations of Na and Mg in the soil layer below 40 cm depth, indicating larger influence of marine water. Isotopic signature of C increased with soil depth suggesting that microorganisms decomposing organic matter are releasing C-13 depleted CO2. Nitrogen isotopic signature decreased with soil depth, indicating enrichment in N-15 possibly as a result of denitrification in the upper soil layers. Mineral elements in leaf tissues showed A. schaueriana with higher concentrations of N, P, Na, K, Cu, Zn, and Na/Ca ratio. Spartina densiflora was characterized by the lowest N and K concentrations, and the highest concentrations of A1 and Fe. Rhizophora mangle and L. racemosa had the highest Ca concentrations. Carbon isotopic signatures identified S. densiflora as a C4 plant, and A. schaueriana as the mangrove species occupying comparatively more water stressed microsites than the rest. Leaf nitrogen isotopic signatures were positive, in correspondence with the soil values. The results support the hypothesis that sites sampled were comparatively fertile, because sediment transport through fresh water run-off is predominant in humid coasts, and therefore plants were not limited by nutrient supply, nor particularly stressed by soil salinity. C1 [Tognella, M. M. P.] UFES, Ctr Univ Norte Espirito Santo CEUNES, Dept Ciencias Agr & Biol, Lab Gestao Manguezal GEMA, Rod BR 101 Norte,Km 60, BR-29934430 Sao Mateus, ES, Brazil. [Soares, M. L. G.] Univ Estado Rio De Janeiro, Nucleo Estudos Manguezais NEMA, Dept Oceanog Biol, Fac Oceanog FAOC, Rua Sao Francisco Xavier,524,Sala 4023-E, BR-22420020 Rio De Janeiro, RJ, Brazil. [Cuevas, E.] Univ Puerto Rico UPR Rio Piedras, Dept Biol, Fac Nat Sci, Ctr Appl Trop Ecol & Conservat, Facundo Bueso Bldg,301-A,POB 23360, San Juan, PR 00931 USA. [Medina, E.] IVIC, Ctr Ecol, Carretera Panamer,Km 11,Edo Miranda,Aptdo 20632, Caracas 1020A, Venezuela. [Medina, E.] USDA Forest Serv, Int Inst Trop Forestry, 1201,Calle Ceiba,Jardin Bot Sur, San Juan, PR 00926 USA. RP Medina, E (reprint author), IVIC, Ctr Ecol, Carretera Panamer,Km 11,Edo Miranda,Aptdo 20632, Caracas 1020A, Venezuela.; Medina, E (reprint author), USDA Forest Serv, Int Inst Trop Forestry, 1201,Calle Ceiba,Jardin Bot Sur, San Juan, PR 00926 USA. EM medinage@gmail.com NR 41 TC 0 Z9 0 U1 13 U2 13 PU INT INST ECOLOGY PI SAO CARLOS PA RUA BENTO CARLOS, 750 - CENTRO, SAO CARLOS, SP 00000, BRAZIL SN 1519-6984 EI 1678-4375 J9 BRAZ J BIOL JI Braz. J. Biol. PD OCT-DEC PY 2016 VL 76 IS 4 BP 994 EP 1003 DI 10.1590/1519-6984.05915 PG 10 WC Biology SC Life Sciences & Biomedicine - Other Topics GA EB8QU UT WOS:000387656700024 PM 27191466 ER PT J AU Santiago, S Roll, DM Ray, C Williams, C Moravcik, P Knopf, A AF Santiago, Sergio Roll, Deborah M. Ray, Chittaranjan Williams, Clinton Moravcik, Philip Knopf, Allan TI Effects of soil moisture depletion on vegetable crop uptake of pharmaceuticals and personal care products (PPCPs) SO ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH LA English DT Article DE Pharmaceuticals and personal care products; PPCPs; Soil moisture depletion; Crop uptake ID WATER TREATMENT PLANTS; ENDOCRINE DISRUPTING COMPOUNDS; ANTIBIOTIC-RESISTANCE GENES; SEWAGE-TREATMENT PLANT; MUNICIPAL WASTE-WATER; RECYCLED WATER; ORGANIC-COMPOUNDS; TERRESTRIAL ENVIRONMENT; AQUATIC CONTAMINATION; RECLAIMED WATER AB Agricultural crops have a long history of being irrigated with recycled wastewater (RW). However, its use on vegetable crops has been of concern due to the potential prevalence of microcontaminants, such as pharmaceuticals and personal care products (PPCPs) in the latter, which represents a possible health hazard to consumers. We investigated the uptake of three PPCPs (atenolol, diclofenac, and ofloxacin), at three different concentrations in irrigation water (0.5, 5, and 25 mu g L-1) in relation to three varying volumetric soil moisture depletion levels of 14 % (-4.26 kPa), 10 % (-8.66 kPa), and 7 % (-18.37 kPa) by various vegetable crop species. Experiments were conducted in a split-split block completely randomized design. PPCPs were extracted using a developed method of accelerated solvent extraction and solid phase extraction and analyzed via liquid chromatography mass spectrometry (LCMS). Results indicate that all treated crops were capable of PPCP uptake at nanogram per gram concentrations independent of the applied soil moisture depletion levels and PPCP concentrations. Ofloxacin was the chemical with the highest uptake amounts, followed by atenolol and then diclofenac. Although the results were not statistically significant, higher concentrations of PPCPs were detected in plants maintained under higher soil moisture levels of 14 % (-4.26 kPa). C1 [Santiago, Sergio; Ray, Chittaranjan] Univ Hawaii, Dept Civil & Environm Engn, Honolulu, HI 96822 USA. [Santiago, Sergio; Ray, Chittaranjan; Moravcik, Philip] Univ Hawaii, Water Resources Res Ctr, Honolulu, HI 96822 USA. [Roll, Deborah M.; Williams, Clinton; Knopf, Allan] ARS, USDA, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Ray, Chittaranjan] Nebraska Water Ctr, Nebraska Innovat Campus, Lincoln, NE 68588 USA. RP Ray, C (reprint author), Univ Hawaii, Dept Civil & Environm Engn, Honolulu, HI 96822 USA.; Ray, C (reprint author), Univ Hawaii, Water Resources Res Ctr, Honolulu, HI 96822 USA.; Ray, C (reprint author), Nebraska Water Ctr, Nebraska Innovat Campus, Lincoln, NE 68588 USA. EM cray@nebraska.edu FU US Department of Agriculture (USDA) Agriculture and Food Research Initiative (AFRI) Grant Accession [224076, HAWR-2010-03561] FX This study was funded by the US Department of Agriculture (USDA) Agriculture and Food Research Initiative (AFRI) Grant Accession No. 224076 (Project No. HAWR-2010-03561) and received institutional support from the University of Hawaii at Manoa, College of Tropical Agriculture and Human Resources (CTAHR). The authors would like to thank Travis Idol, Kheng Cheah, Theodore Radovich, and Halina Zaleski for their substantial contributions throughout the project. NR 102 TC 0 Z9 0 U1 15 U2 15 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0944-1344 EI 1614-7499 J9 ENVIRON SCI POLLUT R JI Environ. Sci. Pollut. Res. PD OCT PY 2016 VL 23 IS 20 BP 20257 EP 20268 DI 10.1007/s11356-016-7194-z PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA EB7VM UT WOS:000387599100019 PM 27447471 ER PT J AU Klick, J Yang, WQ Lee, JC Bruck, DJ AF Klick, Jimmy Yang, Wei Q. Lee, Jana C. Bruck, Denny J. TI Reduced spray programs for Drosophila suzukii management in berry crops SO INTERNATIONAL JOURNAL OF PEST MANAGEMENT LA English DT Article DE Border spray; alternate row middle spray; blueberry; raspberry; natural enemies; fruit knockdown ID SPOTTED-WING DROSOPHILA; COMMERCIAL APPLE ORCHARDS; DIPTERA DROSOPHILIDAE; PLUM CURCULIO; WEEVIL COLEOPTERA; PEST-MANAGEMENT; BORDER SPRAYS; FLIES DIPTERA; CODLING MOTH; INSECTICIDES AB Since the arrival of Drosophila suzukii M. (Diptera: Drosophilidae), field applications of broad-spectrum insecticides have increased in berry crop production to protect susceptible fruits from infestation. Field studies were conducted from 2011 to 2013 to determine whether alternate row middle or border spray programs could manage D. suzukii as well as complete sprays and have less of an impact on non-target arthropods. Four raspberry sites and one blueberry site were alternate row middle and border sprayed, respectively. Adult and larva counts of D. suzukii were compared to conventional complete spray programs. Non-target arthropods were evaluated 7 d post-harvest. In both reduced spray trials, no differences in mean adult numbers and larvae of D. suzukii were detected between treatments. Mean counts immediately after sprays (i.e. 3-12 d after treatment) were also similar in complete and alternate row sprays. Both reduced spray strategies had significantly more Stethorus spp.; additionally, alternate row sprays had significantly more Psyllobora spp. These reduced pesticide strategies are additional tools to consider in D. suzukii IPM programs that can reduce the area sprayed, application time, and input costs while conserving natural enemies. C1 [Klick, Jimmy] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Yang, Wei Q.] Oregon State Univ, Dept Hort, North Willamette Res & Extens Ctr, Aurora, OR USA. [Lee, Jana C.; Bruck, Denny J.] USDA ARS, Hort Crops Res Unit, Corvallis, OR USA. [Klick, Jimmy] Driscoll Strawberry Associates, Oxnard, CA 93033 USA. [Bruck, Denny J.] DuPont Pioneer, Johnston, IA USA. RP Klick, J (reprint author), Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA.; Klick, J (reprint author), Driscoll Strawberry Associates, Oxnard, CA 93033 USA. EM jimmy.klick@driscolls.com FU Oregon State University Agricultural Research Foundation [ARF 4748A]; Oregon Blueberry Commission; WSARE [GW13-014]; USDA SCRI [2010-51181-21167]; USDA CRIS [5358-22000-037-00D] FX Funding was provided by Oregon State University Agricultural Research Foundation [grant number ARF 4748A]; Oregon Blueberry Commission; WSARE [grant number GW13-014]; USDA SCRI [grant number 2010-51181-21167]; and USDA CRIS [grant number 5358-22000-037-00D]. NR 45 TC 0 Z9 0 U1 15 U2 15 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND SN 0967-0874 EI 1366-5863 J9 INT J PEST MANAGE JI Int. J. Pest Manage. PD OCT-DEC PY 2016 VL 62 IS 4 BP 368 EP 377 DI 10.1080/09670874.2016.1222105 PG 10 WC Entomology SC Entomology GA DZ3WY UT WOS:000385781500014 ER PT J AU Li, F Zhuang, H Qiao, WW Zhang, JH Wang, YL AF Li, Feng Zhuang, Hong Qiao, Weiwei Zhang, Jianhao Wang, Yongli TI Effect of partial substitution of NaCl by KCl on physicochemical properties, biogenic amines and N-nitrosamines during ripening and storage of dry-cured bacon SO JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE LA English DT Article DE Dry-cured bacon; Sodium chloride; Potassium chloride; Physicochemical property; Biogenic amines; Nitrosodimethylamine ID PARTIAL SODIUM REPLACEMENT; MEAT-PRODUCTS; FERMENTED SAUSAGES; SENSORY PROPERTIES; POTASSIUM; CHLORIDE; SALTS; MICROBIOLOGY; PROTEOLYSIS; MAGNESIUM AB Effects of three salting treatments (Formulation II: 80 % NaCl + 20 % KCl; Formulation III: 60 % NaCl + 40 % KCl and Formulation IV: 40 % NaCl + 60 % KCl) on physicochemical properties, residual nitrite, N-nitrosamines and biogenic amines were compared with those of control bacons (Formulation I: 100 % NaCl) during processing and storage. Results showed that there were no significant differences among treatments for moisture, pH, and total volatile basic nitrogen (TVBN) content in dry-cured bacons during processing. The replacement of 40 % or less NaCl by KCl had no negative effects on the sensory quality of bacons during processing. Formulation III significantly reduced putrescine, cadaverine and histamine contents and enhanced nitrite residues compared with the control. After 12-day ripening and during storage, the substitution of NaCl by 60 % KCl significantly increased the N-nitrosodimethylamine (NDMA) content than the control. Principal component analysis showed that there were positive correlations between TVBN, biogenic amines (putrescine, cadaverine, histamine and tyramine) and NDMA, and negative correlation between NDMA and nitrite. These findings suggested the partial substitution of NaCl by KCl could be utilized for producing reduced-sodium dry-cured bacons to improve safety of finished products. C1 [Li, Feng; Wang, Yongli] Shandong Agr Univ, Coll Food Sci & Engn, 61 Daizong St, Tai An 271018, Shandong, Peoples R China. [Zhuang, Hong] ARS, Qual & Safety Assessment Res Unit, USDA, Athens, GA 30605 USA. [Qiao, Weiwei; Zhang, Jianhao; Wang, Yongli] Nanjing Agr Univ, Coll Food Sci & Technol, 1 Weigang Rd, Nanjing 210095, Jiangsu, Peoples R China. RP Wang, YL (reprint author), Shandong Agr Univ, Coll Food Sci & Engn, 61 Daizong St, Tai An 271018, Shandong, Peoples R China.; Wang, YL (reprint author), Nanjing Agr Univ, Coll Food Sci & Technol, 1 Weigang Rd, Nanjing 210095, Jiangsu, Peoples R China. EM wylily@sdau.edu.cn FU National Key Technology R&D Program in the 12th Five Year Plan of China [2012BAD28B01]; Science and Technology Foundation in Higher Education of Shandong Province [J16LE18] FX This study was supported by the National Key Technology R&D Program in the 12th Five Year Plan of China (2012BAD28B01) and Science and Technology Foundation in Higher Education of Shandong Province (J16LE18). NR 36 TC 0 Z9 0 U1 5 U2 5 PU SPRINGER INDIA PI NEW DELHI PA 7TH FLOOR, VIJAYA BUILDING, 17, BARAKHAMBA ROAD, NEW DELHI, 110 001, INDIA SN 0022-1155 EI 0975-8402 J9 J FOOD SCI TECH MYS JI J. Food Sci. Technol.-Mysore PD OCT PY 2016 VL 53 IS 10 BP 3795 EP 3805 DI 10.1007/s13197-016-2366-x PG 11 WC Food Science & Technology SC Food Science & Technology GA EB8TB UT WOS:000387662800018 PM 28017995 ER PT J AU Lu, AS Baranowski, T Hong, SL Buday, R Thompson, D Beltran, A Dadabhoy, HR Chen, TA AF Lu, Amy Shirong Baranowski, Tom Hong, S. Lee Buday, Richard Thompson, Debbe Beltran, Alicia Dadabhoy, Hafza Razak Chen, Tzu-An TI The Narrative Impact of Active Video Games on Physical Activity Among Children: A Feasibility Study SO JOURNAL OF MEDICAL INTERNET RESEARCH LA English DT Article DE video games; narration; behavior; child health; child obesity; motivation ID CHILDHOOD OBESITY PREVENTION; ENERGY-EXPENDITURE; BODY-COMPOSITION; HEALTH; INTERVENTIONS; ADOLESCENTS; PREVALENCE; MULTIMEDIA; ADULTHOOD; INSULIN AB Background: Active video games (AVGs) capable of inducing physical activity offer an innovative approach to combating childhood obesity. Unfortunately, children's AVG game play decreases quickly, underscoring the need to identify novel methods for player engagement. Narratives have been demonstrated to influence behaviors. Objective: The objective of this study was to test the hypothesis that a narrative would motivate increased AVG play, though a feasibility study that investigated the motivational effect of adding a previously developed narrative cutscene to an originally nonnarrative AVG, Nintendo Wii Sports Resort: Swordplay Showdown. Methods: A total of 40 overweight and obese 8-to 11-year-olds equally divided by sex played the AVG. Half (n=20) were randomly assigned to a narrative group that watched the narrative cutscene before game play. The other half played the game without watching it. Results: Children in the narrative group had significantly (P<.05) more steps per 10-second period (mean 3.2, SD 0.7) and overall (mean 523, SD 203) during game play compared with the nonnarrative group (10-second period: mean 2.7, SD 0.7; overall: mean 366, SD 172). Conclusions: The AVG with narrative induced increased physical activity. Additional research is needed to understand the mechanisms through which narrative increases physical activity during AVG game play. C1 [Lu, Amy Shirong] Northeastern Univ, Dept Commun Studies, Coll Arts Media & Design, 360 Huntington Ave,204 Lake Hall, Boston, MA 02115 USA. [Lu, Amy Shirong] Northeastern Univ, Dept Hlth Sci, Bouve Coll Hlth Sci, Boston, MA 02115 USA. [Baranowski, Tom; Thompson, Debbe; Beltran, Alicia; Dadabhoy, Hafza Razak] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Hong, S. Lee] Informat Control Co, Columbus, OH USA. [Buday, Richard] Archimage Inc, Houston, TX USA. [Chen, Tzu-An] Univ Texas Hlth Sci Ctr Houston, McGovern Med Sch, Houston, TX 77030 USA. RP Lu, AS (reprint author), Northeastern Univ, Dept Commun Studies, Coll Arts Media & Design, 360 Huntington Ave,204 Lake Hall, Boston, MA 02115 USA. EM a.lu@northeastern.edu OI Baranowski, Tom/0000-0002-0653-2222; Lu, Amy Shirong/0000-0002-8230-9049 FU National Cancer Institute [1R21CA158917-01A1]; US Department of Agriculture's Agricultural Research Service [58-6250-0-008] FX Leia Butler, Leopoldo Castillo, Robert Dolezal, Sakina Kazmi, and Kimberlyn Shirrels helped with data collection in the narrative development stage. This project was funded by a grant from the National Cancer Institute (1R21CA158917-01A1), "The Narrative Impact of Active Video Games on Physical Activity," and federal funds from the US Department of Agriculture's Agricultural Research Service under Cooperative Agreement No. 58-6250-0-008. NR 66 TC 0 Z9 0 U1 14 U2 14 PU JMIR PUBLICATIONS, INC PI TORONTO PA 59 WINNERS CIRCLE, TORONTO, ON M4L 3Y7, CANADA SN 1438-8871 J9 J MED INTERNET RES JI J. Med. Internet Res. PD OCT PY 2016 VL 18 IS 10 BP 101 EP 110 AR e272 DI 10.2196/jmir.6538 PG 10 WC Health Care Sciences & Services; Medical Informatics SC Health Care Sciences & Services; Medical Informatics GA EC2WT UT WOS:000387985700008 PM 27742605 ER PT J AU Roemmich, JN AF Roemmich, James N. TI Height-Adjustable Desks: Energy Expenditure, Liking, and Preference of Sitting and Standing SO JOURNAL OF PHYSICAL ACTIVITY & HEALTH LA English DT Article DE posture; word processing; workplace; workstation; liking ID PHYSICAL-ACTIVITY; NONEXERCISE ACTIVITY; METABOLIC SYNDROME; OBESITY; WORK; EPIDEMIC; BEHAVIOR; TIME AB Background: Breaking periods of sitting with standing may prevent chronic diseases and increase energy expenditure (EE). Sit-to-stand height adjustable desks may promote workplace standing, but workers have to be willing to stand for portions of the workday. Methods: For studies 1 and 2, EE was measured during word processing while sitting in a chair and while standing. Subjects scored their liking of each posture and time they would be willing to work in each posture during an 8-hour workday. Study 2 included an intervention of replacing subjects' sitting desks with a height adjustable desk. Liking of and willingness to work in each posture were measured before and after the 12-month intervention. Results: EE was 7.5 kcal/h greater when standing than when sitting. Subjects liked sitting more than standing in study 1. In study 2, liking of postures did not differ or change across 12 months use of height adjustable desks. Perceived willingness to stand decreased from 4.5 h/d at baseline to 3.4 h/d after 12 months. Conclusions: Standing rather than sitting increased EE by 7.5 kcal/h. Use of a height adjustable desk for 12 months did not alter the hedonic value of standing or sitting, which is promising for long-term increases in standing. C1 [Roemmich, James N.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. RP Roemmich, JN (reprint author), USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. EM james.roemmich@ars.usda.gov FU USDA/Agricultural Research Service [USDA 5450-51000-049-00D, USDA 5450-51000-051-00D] FX The author thanks Joley Beeler, BS, for assistance with writing of the manuscript. Parts of this research were supported by the USDA/Agricultural Research Service, USDA 5450-51000-049-00D and USDA 5450-51000-051-00D. The contents of this publication do not necessarily reflect the views or policies of the USDA or the Agricultural Research Service, nor does mention of trade names, commercial products, or organizations imply endorsement from the US government. No financial disclosures were reported by the authors of this paper. This study is registered at www.clinicaltrials.gov (NCT01787643). NR 29 TC 0 Z9 0 U1 5 U2 5 PU HUMAN KINETICS PUBL INC PI CHAMPAIGN PA 1607 N MARKET ST, PO BOX 5076, CHAMPAIGN, IL 61820-2200 USA SN 1543-3080 EI 1543-5474 J9 J PHYS ACT HEALTH JI J. Phys. Act. Health PD OCT PY 2016 VL 13 IS 10 BP 1094 EP 1099 DI 10.1123/jpah.2015-0397 PG 6 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA EB8ID UT WOS:000387633800010 PM 27256708 ER PT J AU Yu, XQ Li, XR Guo, TT Zhu, CS Wu, YY Mitchell, SE Roozeboom, KL Wang, DH Wang, ML Pederson, GA Tesso, TT Schnable, PS Bernardo, R Yu, JM AF Yu, Xiaoqing Li, Xianran Guo, Tingting Zhu, Chengsong Wu, Yuye Mitchell, Sharon E. Roozeboom, Kraig L. Wang, Donghai Wang, Ming Li Pederson, Gary A. Tesso, Tesfaye T. Schnable, Patrick S. Bernardo, Rex Yu, Jianming TI Genomic prediction contributing to a promising global strategy to turbocharge gene banks SO NATURE PLANTS LA English DT Article ID QUANTITATIVE TRAITS; COMPLEX TRAITS; SELECTION; PRODUCTIVITY; ENVIRONMENT; REGRESSION; MARKERS AB The 7.4 million plant accessions in gene banks are largely underutilized due to various resource constraints, but current genomic and analytic technologies are enabling us to mine this natural heritage. Here we report a proof-of-concept study to integrate genomic prediction into a broad germplasm evaluation process. First, a set of 962 biomass sorghum accessions were chosen as a reference set by germplasm curators. With high throughput genotyping-by-sequencing (GBS), we genetically characterized this reference set with 340,496 single nucleotide polymorphisms (SNPs). A set of 299 accessions was selected as the training set to represent the overall diversity of the reference set, and we phenotypically characterized the training set for biomass yield and other related traits. Cross-validation with multiple analytical methods using the data of this training set indicated high prediction accuracy for biomass yield. Empirical experiments with a 200-accession validation set chosen from the reference set confirmed high prediction accuracy. The potential to apply the prediction model to broader genetic contexts was also examined with an independent population. Detailed analyses on prediction reliability provided new insights into strategy optimization. The success of this project illustrates that a global, cost-effective strategy may be designed to assess the vast amount of valuable germplasm archived in 1,750 gene banks. C1 [Yu, Xiaoqing; Li, Xianran; Guo, Tingting; Zhu, Chengsong; Schnable, Patrick S.; Yu, Jianming] Iowa State Univ, Ames, IA 50011 USA. [Wu, Yuye; Roozeboom, Kraig L.; Wang, Donghai; Tesso, Tesfaye T.] Kansas State Univ, Manhattan, KS 66506 USA. [Mitchell, Sharon E.] Cornell Univ, Ithaca, NY 14853 USA. [Wang, Ming Li; Pederson, Gary A.] ARS, USDA, Griffin, GA 30223 USA. [Bernardo, Rex] Univ Minnesota, St Paul, MN 55108 USA. RP Yu, JM (reprint author), Iowa State Univ, Ames, IA 50011 USA. EM jmyu@iastate.edu FU Agriculture and Food Research Initiative competitive grant from the USDA National Institute of Food and Agriculture [2011-03587]; National Science Foundation [IOS-1238142]; Kansas State University Center for Sorghum Improvement; Iowa State University Raymond F. Baker Center for Plant Breeding; Iowa State University Plant Science Institute FX This work was supported by the Agriculture and Food Research Initiative competitive grant (2011-03587) from the USDA National Institute of Food and Agriculture, by the National Science Foundation grant IOS-1238142, by the Kansas State University Center for Sorghum Improvement, by the Iowa State University Raymond F. Baker Center for Plant Breeding and by the Iowa State University Plant Science Institute. We appreciate K. Mayfield, L. Lambright and S. Staggenborg from Chromatin for conducting experiments at Lubbock, Texas. NR 50 TC 1 Z9 1 U1 13 U2 13 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2055-026X EI 2055-0278 J9 NAT PLANTS JI Nat. Plants PD OCT PY 2016 VL 2 IS 10 AR 16150 DI 10.1038/NPLANTS.2016.150 PG 7 WC Plant Sciences SC Plant Sciences GA EB4AW UT WOS:000387312400003 PM 27694945 ER PT J AU Henry, TJ Howard, SZ AF Henry, Thomas J. Howard, Samuel Z. TI REVISION OF THE NEOTROPICAL PLANT BUG GENUS SINERVUS STAL (HETEROPTERA: MIRIDAE: BRYOCORINAE: ECCRITOTARSINI), WITH THE DESCRIPTION OF FOUR NEW SPECIES AND A CLOSELY RELATED NEW GENUS SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Article DE Insecta; Hemiptera; Miridae; Bryocorinae; new genus; new species; new combinations; Neotropics AB The eccritotarsine plant bug genus Sinervus Stal is revised and the species S. apicalis, n. sp. from Honduras and S. cunealis, n. sp. and S. egeri, n. sp. from Brazil are described, bringing the total species in the genus to 11. Sinervus and the nine previously known species are diagnosed and the three new species are described. The genus Sinervaspartus, n. gen. is described to accommodate Spartacus discovittatus Carvalho, new combination (as the type species), Sinervus espartacoides Carvalho and Gomes, new combination, Spartacus venezuelanus Carvalho, new combination, and Sinervaspartus marginalis, n. sp. from Peru. Color dorsal images for all species, male genitalia for the new species, selected SEM micrographs, and keys to help distinguish Sinervus and Sinervaspartus and all included species are provided. Relationships with the New World genus Spartacus Distant and certain Old World genera are discussed. C1 [Henry, Thomas J.] ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, POB 37012, Washington, DC 20013 USA. [Howard, Samuel Z.] Smithsonian Inst, Natl Museum Nat Hist, Dept Entomol, Washington, DC 20013 USA. [Howard, Samuel Z.] 42 Castro St,Apt 5, San Francisco, CA 94114 USA. RP Henry, TJ (reprint author), ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, POB 37012, Washington, DC 20013 USA. EM thomas.henry@ars.usda.gov NR 21 TC 0 Z9 0 U1 0 U2 0 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD OCT PY 2016 VL 118 IS 4 BP 533 EP 554 DI 10.4289/0013-8797.118.4.533 PG 22 WC Entomology SC Entomology GA EB9SX UT WOS:000387738500005 ER PT J AU Smith, DR Dolan, AC AF Smith, David R. Dolan, Amelia C. TI A NEW SPECIES OF HUCKLEBERRY SAWFLY OF THE FORMER TRIBE PRISTOLINI (HYMENOPTERA: TENTHREDINIDAE) FROM MONTANA SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Article DE Symphyta; Nematinae; Pristolini; Melastola; Pristola; fruit feeders AB Pristiphora fructicola Smith and Dolan, n. sp., is described from Montana. It would belong to the former genus Melastola Wong of the former tribe Pristolini, recently synonymized with Pristiphora Latreille. Adults were numerous around Vaccinium globulare Rydberg (Ericaceae), the presumed host, where larvae feed in the fruits. The species is distinguished from the other two species previously placed in Melastola, P. resinicolor (Marlatt) and P. ferruginosa (Wong) (n. comb.). Several new distribution records and host plant associations are given for the species of "Pristolini." C1 [Smith, David R.] ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, POB 37012,MRC 168, Washington, DC 20013 USA. [Dolan, Amelia C.] Montana State Univ, Montana Entomol Collect, Marsh Labs, Room 50,POB 173145, Bozeman, MT 59717 USA. RP Smith, DR (reprint author), ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, POB 37012,MRC 168, Washington, DC 20013 USA. EM sawfly2@aol.com; amelia.clare1229@gmail.com FU Montana Department of Agriculture's Specialty Crop Block Grant Program FX We thank Chris Grinter, Illinois Natural History Survey, Champaign, IL, for loan of the type slide of Melastola ferruginosa and Derek Sikes, UAM, for loan of specimens. Michael Ivie, Montana State University, assisted in the determination of this species and loaned specimens for study. Paloma Amaral assisted with field work. This work was conducted with funding to Michael Ivie from a grant by the Montana Department of Agriculture's Specialty Crop Block Grant Program titled "Huckleberry Challenges: Pollinator Mysteries, Pests and New Invasive Threats." Work in the Custer Gallatin National Forest was conducted with permission of Mary C. Erickson, Forest Supervisor, U. S. National Forest Service, Bozeman. This is a contribution of the Montana Agricultural Experiment Station. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer. NR 7 TC 0 Z9 0 U1 1 U2 1 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD OCT PY 2016 VL 118 IS 4 BP 594 EP 601 DI 10.4289/0013-8797.118.4.594 PG 8 WC Entomology SC Entomology GA EB9SX UT WOS:000387738500010 ER PT J AU Smith, DR Strazanac, JS AF Smith, David R. Strazanac, John S. TI ANNOTATED LIST OF WEST VIRGINIA SAWFLIES (HYMENOPTERA: SYMPHYTA) SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Article DE species list; Xyelidae; Pamphiliidae; Pergidae; Argidae; Cimbicidae; Diprionidae; Tenthredinidae; Siricidae; Xiphydriidae; Orussidae; Cephidae ID EASTERN UNITED-STATES; QUERCUSCOCCINEAE DYAR HYMENOPTERA; NEMATUS PANZER HYMENOPTERA; NORTH-AMERICA; CENTRAL KENTUCKY; HOST PLANTS; TENTHREDINIDAE; GENUS; WASHINGTON; ARGIDAE AB Records for 281 species of sawflies in 11 families are listed for West Virginia. Results are based on extensive Malaise trap collections at several sites in Hardy, Tucker, and Pocohantas counties and data from museum specimens. County records and recorded host plants are given for each species. C1 [Smith, David R.] ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, POB 37012,MRC 168, Washington, DC 20013 USA. [Strazanac, John S.] West Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA. RP Smith, DR (reprint author), ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, POB 37012,MRC 168, Washington, DC 20013 USA. EM sawfly2@aol.com; fritzstraz@gmail.com NR 73 TC 0 Z9 0 U1 0 U2 0 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD OCT PY 2016 VL 118 IS 4 BP 602 EP 616 DI 10.4289/0013-8797.118.4.602 PG 15 WC Entomology SC Entomology GA EB9SX UT WOS:000387738500011 ER PT J AU Adaime, R de Jesus-Barros, CR Uramoto, K Norrbom, AL Zucchi, RA AF Adaime, Ricardo de Jesus-Barros, Cristiane Ramos Uramoto, Keiko Norrbom, Allen L. Zucchi, Roberto Antonio TI First Record of Anastrepha zacharyi Norrbom (Diptera, Tephritidae) in Brazil, and Notes on its Host Plant and Parasitoid SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Editorial Material ID HYMENOPTERA; STATE C1 [Adaime, Ricardo; de Jesus-Barros, Cristiane Ramos] Embrapa Amapa, BR-68903419 Macapa, Amapa, Brazil. [Uramoto, Keiko; Zucchi, Roberto Antonio] Univ Sao Paulo, Dept Entomol & Acarol, Escola Super Agr Luiz de Queiroz, BR-13418900 Sao Paulo, Brazil. [Norrbom, Allen L.] ARS, Systemat Entomol Lab, USDA, Smithsonian Inst, POB 37012,MRC 168, Washington, DC 20013 USA. RP Adaime, R (reprint author), Embrapa Amapa, BR-68903419 Macapa, Amapa, Brazil. EM ricardo.adaime@embrapa.br NR 15 TC 0 Z9 0 U1 2 U2 2 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD OCT PY 2016 VL 118 IS 4 BP 636 EP 640 DI 10.4289/0013-8797.118.4.636 PG 5 WC Entomology SC Entomology GA EB9SX UT WOS:000387738500014 ER PT J AU Karlen, SD Zhang, CC Peck, ML Smith, RA Padmakshan, D Helmich, KE Free, HCA Lee, S Smith, BG Lu, FC Sedbrook, JC Sibout, R Grabber, JH Runge, TM Mysore, KS Harris, PJ Bartley, LE Ralph, J AF Karlen, Steven D. Zhang, Chengcheng Peck, Matthew L. Smith, Rebecca A. Padmakshan, Dharshana Helmich, Kate E. Free, Heather C. A. Lee, Seonghee Smith, Bronwen G. Lu, Fachuang Sedbrook, John C. Sibout, Richard Grabber, John H. Runge, Troy M. Mysore, Kirankumar S. Harris, Philip J. Bartley, Laura E. Ralph, John TI Monolignol ferulate conjugates are naturally incorporated into plant lignins SO SCIENCE ADVANCES LA English DT Article ID CELL-WALLS; STRUCTURAL-CHARACTERIZATION; TRANSFERASE PMT; DFRC METHOD; RICE; LIGNIFICATION; OVEREXPRESSION; IDENTIFICATION; BIOSYNTHESIS; PERSPECTIVE AB Angiosperms represent most of the terrestrial plants and are the primary research focus for the conversion of biomass to liquid fuels and coproducts. Lignin limits our access to fibers and represents a large fraction of the chemical energy stored in plant cell walls. Recently, the incorporation of monolignol ferulates into lignin polymers was accomplished via the engineering of an exotic transferase into commercially relevant poplar. We report that various angiosperm species might have convergently evolved to natively produce lignins that incorporate monolignol ferulate conjugates. We show that this activity may be accomplished by a BAHD feruloyl-coenzyme A monolignol transferase, OsFMT1 (AT5), in rice and its orthologs in other monocots. C1 [Karlen, Steven D.; Smith, Rebecca A.; Padmakshan, Dharshana; Helmich, Kate E.; Harris, Philip J.; Ralph, John] Univ Wisconsin, Wisconsin Energy Inst, Great Lakes Bioenergy Res Ctr, Dept Energy, Madison, WI 53726 USA. [Karlen, Steven D.; Smith, Rebecca A.; Helmich, Kate E.; Lu, Fachuang; Ralph, John] Univ Wisconsin, Dept Bio Chem, Madison, WI 53706 USA. [Zhang, Chengcheng; Peck, Matthew L.; Bartley, Laura E.] Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK 73019 USA. [Free, Heather C. A.; Harris, Philip J.] Univ Auckland, Sch Biol Sci, Auckland, New Zealand. [Free, Heather C. A.; Smith, Bronwen G.] Univ Auckland, Sch Chem Sci, Auckland, New Zealand. [Lee, Seonghee] Univ Florida, IFAS, Dept Hort Sci, Gulf Coast Res & Educ Ctr, 14625 Cty Rd 672, Wimauma, FL 33598 USA. [Grabber, John H.] Illinois State Univ, Sch Biol Sci, Dept Energy Great Lakes Bioenergy Res Ctr, Normal, IL 61790 USA. [Smith, Bronwen G.; Sedbrook, John C.; Grabber, John H.] AgroParisTech, Inst Jean Pierre Bourgin, UMR 1318, Saclay Plant Sci, F-78000 Versailles, France. [Peck, Matthew L.; Free, Heather C. A.; Sibout, Richard; Grabber, John H.] Inst Natl Rech Agronom, F-78000 Versailles, France. [Helmich, Kate E.; Free, Heather C. A.; Lu, Fachuang; Grabber, John H.] ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Free, Heather C. A.; Sibout, Richard] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA. [Smith, Rebecca A.; Lee, Seonghee] Samuel Roberts Noble Fdn Inc, Plant Biol Div, Ardmore, OK 73401 USA. RP Ralph, J (reprint author), Univ Wisconsin, Wisconsin Energy Inst, Great Lakes Bioenergy Res Ctr, Dept Energy, Madison, WI 53726 USA.; Ralph, J (reprint author), Univ Wisconsin, Dept Bio Chem, Madison, WI 53706 USA.; Bartley, LE (reprint author), Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK 73019 USA. EM lbartley@ou.edu; jralph@wisc.edu NR 43 TC 1 Z9 1 U1 6 U2 6 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 2375-2548 J9 SCI ADV JI Sci. Adv. PD OCT PY 2016 VL 2 IS 10 AR e1600393 DI 10.1126/sciadv.1600393 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EC2YV UT WOS:000387991500008 ER PT J AU Shi, GJ Zhang, ZC Friesen, TL Raats, D Fahima, T Brueggeman, RS Lu, SW Trick, HN Liu, ZH Chao, W Frenkel, Z Xu, SS Rasmussen, JB Faris, JD AF Shi, Gongjun Zhang, Zengcui Friesen, Timothy L. Raats, Dina Fahima, Tzion Brueggeman, Robert S. Lu, Shunwen Trick, Harold N. Liu, Zhaohui Chao, Wun Frenkel, Zeev Xu, Steven S. Rasmussen, Jack B. Faris, Justin D. TI The hijacking of a receptor kinase-driven pathway by a wheat fungal pathogen leads to disease SO SCIENCE ADVANCES LA English DT Article ID PYRENOPHORA-TRITICI-REPENTIS; RESISTANCE GENE; PROTEIN; SYSTEM; TOXA; SUSCEPTIBILITY; INFECTION; EFFECTOR; REVEALS; PLANTS AB Necrotrophic pathogens live and feed on dying tissue, but their interactions with plants are not well understood compared to biotrophic pathogens. The wheat Snn1 gene confers susceptibility to strains of the necrotrophic pathogen Parastagonospora nodorum that produce the SnTox1 protein. We report the positional cloning of Snn1, a member of the wall-associated kinase class of receptors, which are known to drive pathways for biotrophic pathogen resistance. Recognition of SnTox1 by Snn1 activates programmed cell death, which allows this necrotroph to gain nutrients and sporulate. These results demonstrate that necrotrophic pathogens such as P. nodorum hijack host molecular pathways that are typically involved in resistance to biotrophic pathogens, revealing the complex nature of susceptibility and resistance in necrotrophic and biotrophic pathogen interactions with plants. C1 [Shi, Gongjun; Friesen, Timothy L.; Brueggeman, Robert S.; Liu, Zhaohui; Rasmussen, Jack B.] North Dakota State Univ, Dept Plant Pathol, Fargo, ND 58102 USA. [Zhang, Zengcui; Friesen, Timothy L.; Lu, Shunwen; Xu, Steven S.; Faris, Justin D.] ARS, USDA, Cereal Crops Res Unit, Red River Valley Agr Res Ctr, Fargo, ND 58102 USA. [Raats, Dina; Fahima, Tzion; Frenkel, Zeev] Univ Haifa, Inst Evolut, IL-3498838 Haifa, Israel. [Raats, Dina; Fahima, Tzion; Frenkel, Zeev] Univ Haifa, Dept Evolutionary & Environm Biol, IL-3498838 Haifa, Israel. [Trick, Harold N.] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Chao, Wun] ARS, USDA, Sunflower & Plant Biol Res Unit, Red River Valley Agr Res Ctr, Fargo, ND 58102 USA. RP Faris, JD (reprint author), ARS, USDA, Cereal Crops Res Unit, Red River Valley Agr Res Ctr, Fargo, ND 58102 USA. EM justin.faris@ars.usda.gov NR 36 TC 2 Z9 2 U1 5 U2 5 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 2375-2548 J9 SCI ADV JI Sci. Adv. PD OCT PY 2016 VL 2 IS 10 AR e1600822 DI 10.1126/sciadv.1600822 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EC2YV UT WOS:000387991500018 PM 27819043 ER PT J AU Berstis, L Elder, T Crowley, M Beckham, GT AF Berstis, Laura Elder, Thomas Crowley, Michael Beckham, Gregg T. TI Radical Nature of C-Lignin SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Lignin biosynthesis; Lignin valorization; Caffeoyl alcohol; Caffeyl alcohol; Bond dissociation enthalpy ID BOND-DISSOCIATION ENTHALPIES; MODEL COMPOUNDS; LIGNOCELLULOSIC BIOMASS; BETA-GUAIACYL; NONCOVALENT INTERACTIONS; FUNCTIONAL MATERIALS; PHYSICAL-PROPERTIES; CRYSTAL-STRUCTURE; KRAFT LIGNIN; BIOSYNTHESIS AB The recently discovered lignin composed of caffeoyl alcohol monolignols or C-lignin is particularly intriguing given its homogeneous, linear polymeric structure and exclusive benzodioxane linkage between monomers. By virtue of this simplified chemistry, the potential emerges for improved valorization strategies with C-lignin relative to other natural heterogeneous lignins. To better understand caffeoyl alcohol polymers, we characterize the thermodynamics of the radical recombination dimerization reactions forming the benzodioxane linkage and the bond dissociation into radical monolignol products. These properties are also predicted for the cross-coupling of caffeoyl alcohol with the natural monolignols, coniferyl alcohol, sinapyl alcohol, and p-coumaryl alcohol, in anticipation of polymers potentially enabled by genetic modification. The average BDEs for the C-lignin benzodioxane alpha- and beta-bonds are 56.5 and 63.4 kcal/mol, respectively, with similar enthalpies for heterodimers. The BDE of the alpha-bond within the benzodioxane linkage is consistently greater than that of the beta-bond in all dimers of each stereochemical arrangement, explained by the ability the alpha-carbon radical generated to delocalize onto the adjacent phenyl ring. Relative thermodynamics of the heterodimers demonstrates that the substituents on the phenyl ring directly neighboring the bond coupling the monolignols more strongly impact the dimer bond strengths and product stability, compared to the substituents present on the terminal phenyl ring. Enthalpy comparisons furthermore demonstrate that the erythro stereochemical configurations of the benzodioxane bond are slightly less thermodynamically stable than the threo configurations. The overall differences in strength of bonds and reaction enthalpies between stereoisomers are generally found to be insignificant, supporting that postcoupling rearomatization is under kinetic control. Projecting the lowest-energy stereoisomer internal coordinates to longer polymer C-lignin strands highlights how significantly the stereochemical outcomes in polymerization may impact the macromolecular structure and in turn material and chemical properties. Through these comparisons of geometry, bond strengths, and reaction enthalpies, we shed light on the distinctive properties of C-lignin's radical recombination and decomposition chemistry, and its potential as a natural lignin solution for biorefinery feedstocks and unique materials science applications. C1 [Berstis, Laura; Crowley, Michael; Beckham, Gregg T.] Natl Bioenergy Ctr, Natl Renewable Energy Lab, 15013 Denver West Pkwy, Golden, CO 80401 USA. [Elder, Thomas] USDA, Forest Serv, Southern Res Stn, 521 Devall Dr, Auburn, AL 36849 USA. RP Crowley, M; Beckham, GT (reprint author), Natl Bioenergy Ctr, Natl Renewable Energy Lab, 15013 Denver West Pkwy, Golden, CO 80401 USA. EM Michael.Crowley@nrel.gov; Gregg.Beckham@nrel.gov FU US Department of Energy Bioenergy Technologies Office; DOE Office of EERE [DE-AC36-08GO28308] FX This work was supported by the US Department of Energy Bioenergy Technologies Office. We are grateful for supercomputer time on Stampede provided by the Texas Advanced Computing Center (TACC) at the University of Texas at Austin through MCB-09159 and the NREL Computational Sciences Center, which is supported by the DOE Office of EERE under Contract No. DE-AC36-08GO28308. NR 63 TC 0 Z9 0 U1 5 U2 5 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD OCT PY 2016 VL 4 IS 10 BP 5327 EP 5335 DI 10.1021/acssuschemeng.6b00520 PG 9 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA DY0MS UT WOS:000384791500024 ER PT J AU Abernethy, EF Turner, KL Beasley, JC DeVault, TL Pitt, WC Rhodes, OE AF Abernethy, Erin F. Turner, Kelsey L. Beasley, James C. DeVault, Travis L. Pitt, William C. Rhodes, Olin E., Jr. TI Carcasses of invasive species are predominantly utilized by invasive scavengers in an island ecosystem SO ECOSPHERE LA English DT Article DE cane toads; cannibalism; carrion; ecosystem function; Hawai'i; invasive species; mongoose; scavenging ID UNGULATE CARCASSES; AVIAN SCAVENGERS; CARRION; DECOMPOSITION; IMPACTS; SUCCESSION; PREDATORS; AUSTRALIA; NITROGEN; BIOLOGY AB Invasive species have significantly affected ecosystems, particularly islands, and species invasions continue with increasing globalization. Largely unstudied, the influence of invasive species on island ecosystem functions, especially scavenging and decomposition, could be substantive. Quantifying carcass utilization by different scavengers and shifts in community dynamics in the presence of invasive animals is of particular interest for understanding impacts on nutrient recycling. Invasive species could benefit greatly from carcass resources within highly invaded island ecosystems, through increased invasion success and population growth, subsequently exacerbating their impacts on native species. We quantified how experimentally placed invasive amphibian, reptile, small mammal, and bird carcasses were utilized by vertebrate and invertebrate scavengers on the Big Island of Hawai'i in three island habitats: a barren lava field, a vegetated lava field, and a rainforest. We used camera traps to record vertebrate scavengers removing carcasses and elapsed time until removal. We evaluated differences in scavenging between vertebrates and invertebrates and within the vertebrate community across different habitats and carcass types. Despite the small carcass sizes (<1 kg) used in this study, 55% of carcasses were removed by vertebrate scavengers, all invasive: mongoose, rodents, cats, pigs, and a common myna. Our data indicate that invasive vertebrate scavengers in this island ecosystem are highly efficient at assimilating a range of carrion resources across a variety of habitats. Carcasses of invasive animals could contribute substantially to energy budgets of other invasive vertebrate species. This may be a critical component contributing to successful invasions especially on islands and subsequent impacts on ecosystem function. C1 [Abernethy, Erin F.] Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. [Abernethy, Erin F.; Turner, Kelsey L.; Beasley, James C.; Rhodes, Olin E., Jr.] Univ Georgia, Savannah River Ecol Lab, Aiken, SC 29802 USA. [Turner, Kelsey L.; Beasley, James C.] Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. [DeVault, Travis L.] USDA APHIS, Natl Wildlife Res Ctr, Sandusky, OH 44870 USA. [Pitt, William C.] USDA APHIS, Natl Wildlife Res Ctr, Hilo, HI 96720 USA. [Abernethy, Erin F.] Oregon State Univ, Dept Integrat Biol, Corvallis, OR 97331 USA. [Pitt, William C.] Natl Zool Pk, Smithsonian Conservat Biol Inst, Front Royal, VA 22630 USA. RP Abernethy, EF (reprint author), Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA.; Abernethy, EF (reprint author), Univ Georgia, Savannah River Ecol Lab, Aiken, SC 29802 USA.; Abernethy, EF (reprint author), Oregon State Univ, Dept Integrat Biol, Corvallis, OR 97331 USA. EM efabernethy@gmail.com FU University of Georgia Research Foundation; USDA NWRC Hilo Field Station [12-7415-0936-CA]; US Department of Energy [DE-FC09-07SR22506]; agency of the US Government FX We appreciate the field assistance of Shem Unger. We sincerely thank Kelton Kotake, Bob Sugihara, Dean Foster, Tom McAuliffe, and Aaron Shiels at the USDA National Wildlife Research Center Hilo Field Station for making this study possible. We thank Hawai'i Volcanoes National Park and the Natural Area Reserve System of HI DLNR for giving us a location and permit to conduct our study. This work was supported through Cooperative Agreements among the University of Georgia Research Foundation, the USDA NWRC Hilo Field Station (No. 12-7415-0936-CA), and the US Department of Energy (No. DE-FC09-07SR22506). This paper was prepared as an account of work sponsored by an agency of the US Government. Neither the US Government nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement, recommendation, or favoring by the US Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the US Government or any agency thereof. NR 61 TC 0 Z9 0 U1 15 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD OCT PY 2016 VL 7 IS 10 AR e01496 DI 10.1002/ecs2.1496 PG 15 WC Ecology SC Environmental Sciences & Ecology GA EB2TU UT WOS:000387216300034 ER PT J AU Conner, MM Keane, JJ Gallagher, CV Munton, TE Shaklee, PA AF Conner, Mary M. Keane, John J. Gallagher, Claire V. Munton, Thomas E. Shaklee, Paula A. TI Comparing estimates of population change from occupancy and mark-recapture models for a territorial species SO ECOSPHERE LA English DT Article DE Bayesian MCMC approach; California spotted owl; mark-resight; monitoring; occupancy model; Pradel's temporal symmetry model; realized population change; Sierra Nevada; Strix occidentalis occidentalis ID ESTIMATING SITE OCCUPANCY; CALIFORNIA SPOTTED OWL; ESTIMATING ABUNDANCE; BAYESIAN-APPROACH; LOCAL EXTINCTION; GROWTH RATE; DYNAMICS; TRENDS; COLONIZATION; INFERENCE AB Monitoring studies often use marked animals to estimate population abundance at small spatial scales. However, at smaller scales, occupancy sampling, which uses detection/nondetection data, may be useful where sites are approximately territories, and occupancy dynamics should be strongly correlated with population dynamics. Occupancy monitoring has advantages in that it is less expensive and invasive, and marked animals are not needed. Here, we used empirical data to determine whether and when change in occupancy is a good proxy for population change for a territorial species. As part of this overall goal, we also compared maximum-likelihood estimates using a model-averaging approach with a Bayesian MCMC approach. We used field data collected from 1993 to 2013 on three study areas for California spotted owls (Strix occidentalis occidentalis), a territorial species. Although correlations for trajectories of realized population change (Delta(t)) between territory occupancy and Pradel models were moderate to high for Bayesian MCMC-based estimates and high for model-averaged estimates, magnitudes of the trajectories were different with the Pradel model reporting greater magnitudes of change. For the two areas showing a decline, Delta(t) for the Pradel model was approximately 20-30% lower than for the occupancy model, and 25% higher in the area showing an increase. These differences can arise because the occupancy model is less sensitive, in that if two owls share a territory, the loss of one may be reflected in survival and, consequently in Delta(t) by the Pradel model, but because the territory remains occupied it is not reflected by the occupancy model. Bayesian MCMC-based and model-averaged estimates of Delta(t) were in close agreement in pattern (correlation >= 0.74) and magnitude (relative differences of last Delta(t) were <= 5%) for both occupancy and mark-resight models. Results from the Pradel model may lead to conservation actions necessary to avoid high extinction or extirpation risk for small populations, while results from the territory occupancy model may result in status quo management. We found both Bayesian MCMC-based and model-averaged estimates of Delta(t) robust approaches to evaluate population trends. However, we recommend the Bayesian MCMC approach for estimating risk (e.g., probability of declines) for retrospective analyses. C1 [Conner, Mary M.; Keane, John J.; Gallagher, Claire V.; Shaklee, Paula A.] US Forest Serv, USDA, Pacific Southwest Res Stn, 1731 Res Pk Dr, Davis, CA 95618 USA. [Munton, Thomas E.] US Forest Serv, USDA, Pacific Southwest Res Stn, 2081 East Sierra Ave, Fresno, CA 93710 USA. [Conner, Mary M.] Utah State Univ, Dept Wildland Resources, 5320 Old Main Hill, Logan, UT 84322 USA. RP Conner, MM (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, 1731 Res Pk Dr, Davis, CA 95618 USA.; Conner, MM (reprint author), Utah State Univ, Dept Wildland Resources, 5320 Old Main Hill, Logan, UT 84322 USA. EM mary.conner@usu.edu FU U.S. Forest Service Region 5, the Pacific Southwest Research Station FX We thank the many field technicians who carefully collected data over the 20 yr of the study. In particular, we thank those who ran field projects were involved for the long haul (> 7 yr), or helped with data analyses including George Steger, Jennifer Blakesley, Dan Shaw, and Rick Scherer, Susan Sutton-Mazzocco, Lori Werner, Mason Werner, Kevin Mazzocco, Kenneth Johnson, Rachel Kussow, M. Warner, and Alfred Peet for their efforts in the field and out, which resulted in such a high-quality and long-term data set. We thank Michael Ebinger for providing invaluable help with the MATLAB code we used to generate our encounter histories. We thank Angela White, Seth Bigelow, Matthew Bokach, Ken Hubbert, and Tray Biasiolli, for helpful comments on drafts of the manuscript. Finally, we thank Brian Halstead for an exceptionally insightful review. Funding for our analysis was provided primarily for U.S. Forest Service Region 5, the Pacific Southwest Research Station. NR 60 TC 0 Z9 0 U1 13 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD OCT PY 2016 VL 7 IS 10 AR e01538 DI 10.1002/ecs2.1538 PG 20 WC Ecology SC Environmental Sciences & Ecology GA EB2TU UT WOS:000387216300062 ER PT J AU Endress, BA Naylor, BJ Pekin, BK Wisdom, MJ AF Endress, Bryan A. Naylor, Bridgett J. Pekin, Burak K. Wisdom, Michael J. TI Aboveground and belowground mammalian herbivores regulate the demography of deciduous woody species in conifer forests SO ECOSPHERE LA English DT Article DE cattle; elk; grazing; North America; Oregon; plant population dynamics; plant-herbivore interactions; Populus trichocarpa; Salix scouleriana; trophic cascades; ungulate herbivory ID UNGULATE HERBIVORY; PLANT-COMMUNITIES; PRIMARY PRODUCTIVITY; INSECT HERBIVORY; BOTTOM-UP; TOP-DOWN; RECRUITMENT; VEGETATION; ECOSYSTEMS; RESPONSES AB Mammalian herbivory can have profound impacts on plant population and community dynamics. However, our understanding of specific herbivore effects remains limited, even in regions with high densities of domestic and wild herbivores, such as the semiarid conifer forests of western North America. We conducted a seven-year manipulative experiment to evaluate the effects of herbivory by two common ungulates, Cervus elaphus Rocky Mountain elk) and cattle Bos taurus (domestic cattle) on growth and survival of two woody deciduous species, Populus trichocarpa (cottonwood) and Salix scouleriana (Scouler's willow) in postfire early-successional forest stands. Additionally, we monitored belowground herbivory by Thomomys talpoides (pocket gopher) and explored effects of both aboveground and belowground herbivory on plant vital rates. Three, approximately 7 ha exclosures were constructed, and each was divided into 1-ha plots. Seven herbivory treatments were then randomly assigned to the plots: three levels of herbivory (low, moderate, and high) for both cattle and elk, and one complete ungulate exclusion treatment. Treatments were implemented for seven years. Results showed that cattle and elk substantially reduced height and growth of both cottonwood and willow. Elk had a larger effect on growth and subsequent plant height than cattle, especially for cottonwood, and elk effects occurred even at low herbivore densities. Pocket gophers had a strong effect on survival of both plant species while herbivory by ungulates did not. However, we documented significant interaction effects of aboveground and belowground herbivory on survival. Our study is one of the first to evaluate top-down regulation by multiple herbivore species at varying densities. Results suggest that traditional exclosure studies that treat herbivory as a binary factor (either present or absent) may not be sufficient to characterize top-down regulation on plant demography. Rather, the strength of top-down regulation varies depending on a number of factors including herbivore species, herbivore density, interactions among multiple herbivore species, and varying tolerance levels of different plant species to herbivory. C1 [Endress, Bryan A.] Oregon State Univ, Dept Anim & Rangeland Sci, Eastern Oregon Agr & Nat Resource Program, La Grande, OR 97850 USA. [Naylor, Bridgett J.; Wisdom, Michael J.] US Forest Serv, USDA, Pacific Northwest Res Stn, La Grande, OR 97850 USA. [Pekin, Burak K.] Istanbul Tech Univ, Eurasia Inst Earth Sci, TR-34469 Istanbul, Turkey. RP Endress, BA (reprint author), Oregon State Univ, Dept Anim & Rangeland Sci, Eastern Oregon Agr & Nat Resource Program, La Grande, OR 97850 USA. EM bryan.endress@oregonstate.edu FU USDA Forest Service Pacific Norwest Research Station, Blue Mountains Demonstration Project and Oregon State University FX We are grateful for the 12 summer technicians who helped collect field data for our research. We also thank Jennifer Hafer, Kent Coe, Brian Dick, Ryan Kennedy, and Dennis Rea for support on all aspects of the research and Tim DelCurto, Martin Vavra, Abe Clark, John Cook, and Rachael Cook for designing and implementing the cattle and elk herbivory treatments. Research was funded by the USDA Forest Service Pacific Norwest Research Station, Blue Mountains Demonstration Project and Oregon State University. Research was conducted under approval and guidance by an Institutional Animal Care and Use Committee (IACUC 92-F-0004), as required by the United States Animal Welfare Act of 1985 and its regulations. We followed protocols established specifically by the IACUC for conducting cattle and elk research in our study area (Wisdom et al. 1993). NR 71 TC 0 Z9 0 U1 8 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD OCT PY 2016 VL 7 IS 10 AR e01530 DI 10.1002/ecs2.1530 PG 18 WC Ecology SC Environmental Sciences & Ecology GA EB2TU UT WOS:000387216300056 ER PT J AU Fay, PA Guntenspergen, GR Olker, JH Johnson, WC AF Fay, Philip A. Guntenspergen, Glenn R. Olker, Jennifer H. Johnson, W. Carter TI Climate change impacts on freshwater wetland hydrology and vegetation cover cycling along a regional aridity gradient SO ECOSPHERE LA English DT Article DE ecosystem models; ecosystem services; grasslands; Prairie Pothole Region; precipitation gradient; wetland complexes; wetland conservation; WETLANDSCAPE ID PRAIRIE POTHOLE REGION; DYNAMICS AB Global mean temperature may increase up to 6 degrees C by the end of this century and together with precipitation change may steepen regional aridity gradients. The hydrology, productivity, and ecosystem services from freshwater wetlands depend on their future water balance. We simulated the hydrology and vegetation dynamics of wetland complexes in the North American Prairie Pothole Region with the WETLANDSCAPE model. Simulations for 63 precipitation x temperature combinations spanning 6 degrees C warming and - 20% to + 20% annual precipitation change at 19 locations along a mid-continental aridity gradient showed that aridity explained up to 99% of the variation in wetland stage and hydroperiod for all wetland permanence types, and in vegetation cycling for semipermanent wetlands. The magnitude and direction of hydrologic responses depended on whether climate changes increased or decreased water deficits. Warming to 6 degrees C and 20% less precipitation increased wetland water deficits and more strongly decreased wetland stage and hydroperiod from historic levels at low aridity, especially in semipermanent wetlands, where peak vegetation cycling (Cover Cycle Index, CCI) also shifted to lower aridity. In contrast, 20% more precipitation decreased water deficits, increasing wetland stage and hydroperiod most strongly in shallow wetlands at high aridity, but filling semipermanent wetlands and reducing CCI at low aridity. All climate changes narrowed the range of aridity favorable to high productivity. Climate changes that reduce water deficits may help maintain wetlands at high aridity at the expense of those at low aridity, but with warming certain, increased deficits are more likely and will help maintain wetlands at lower aridity but exacerbate loss of wetlands at high aridity. Thus, there is likely not a universally applicable approach to mitigating climate change impacts on freshwater wetlands across regional aridity gradients. Conservation strategies need to account for aridity-specific effects of climate change on freshwater wetland ecosystems. C1 [Fay, Philip A.] USDA ARS, Grassland Soil & Water Res Lab, 808 E Blackland Rd, Temple, TX 76502 USA. [Guntenspergen, Glenn R.] Patuxent Wildlife Res Ctr, USGS, Laurel, MD 20708 USA. [Olker, Jennifer H.] Univ Minnesota Duluth, Nat Resources Res Inst, 5013 Miller Trunk Highway, Duluth, MN 55811 USA. [Johnson, W. Carter] South Dakota State Univ, Dept Nat Resource Management, Brookings, SD 57007 USA. RP Fay, PA (reprint author), USDA ARS, Grassland Soil & Water Res Lab, 808 E Blackland Rd, Temple, TX 76502 USA. EM philip.fay@ars.usda.gov FU National Science Foundation [1339944, 1340413]; U.S. Geological Survey Climate and Land-Use Research and Development Program; USDA-Agricultural Research Service Climate; Air and Soils National Program; USDA-NIFA [2010-12865615-20632] FX We thank Anne Gibson for generating the weather scenarios and acknowledge funding from the National Science Foundation (Behavioral and Cognitive Sciences 1339944, Emerging Frontiers 1340413), U.S. Geological Survey Climate and Land-Use Research and Development Program, USDA-Agricultural Research Service Climate, Air and Soils National Program, and USDA-NIFA (2010-12865615-20632). Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. USDA is an Equal Opportunity Employer. The authors declare no conflict of interests. NR 29 TC 0 Z9 0 U1 12 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD OCT PY 2016 VL 7 IS 10 AR e01504 DI 10.1002/ecs2.1504 PG 12 WC Ecology SC Environmental Sciences & Ecology GA EB2TU UT WOS:000387216300042 ER PT J AU Monahan, WB Rosemartin, A Gerst, KL Fisichelli, NA Ault, T Schwartz, MD Gross, JE Weltzin, JF AF Monahan, William B. Rosemartin, Alyssa Gerst, Katharine L. Fisichelli, Nicholas A. Ault, Toby Schwartz, Mark D. Gross, John E. Weltzin, Jake F. TI Climate change is advancing spring onset across the US national park system SO ECOSPHERE LA English DT Article DE climate change; landscape context; monitoring; national parks; phenology; protected areas; Special Feature: Science for Our National Parks' Second Century; spring index; United States ID UNITED-STATES; PHENOLOGICAL RESPONSE; FLOWERING PHENOLOGY; PLANT PHENOLOGY; MANAGEMENT; INDEXES; DESERT; TRENDS; FROST AB Many U.S. national parks are already at the extreme warm end of their historical temperature distributions. With rapidly warming conditions, park resource management will be enhanced by information on seasonality of climate that supports adjustments in the timing of activities such as treating invasive species, operating visitor facilities, and scheduling climate-related events (e.g., flower festivals and fall leaf-viewing). Seasonal changes in vegetation, such as pollen, seed, and fruit production, are important drivers of ecological processes in parks, and phenology has thus been identified