FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Myers, SW Cancio-Martinez, E Hallman, GJ Fontenot, EA Vreysen, MJB AF Myers, Scott W. Cancio-Martinez, Elena Hallman, Guy J. Fontenot, Emily A. Vreysen, Marc J. B. TI Relative Tolerance of Six Bactrocera (Diptera: Tephritidae) Species to Phytosanitary Cold Treatment SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE fruit fly; invasive species; quarantine treatment ID MEDITERRANEAN FRUIT-FLY; CERATITIS-CAPITATA; DISINFESTATION; INVADENS; ORANGES; LARVAE; EGGS AB To compare relative cold treatment tolerance across the economically important tephritid fruit flies (Diptera: Tephritidae), Bactrocera carambolae Drew & Hancock, Bactrocera correcta (Bezzi), Bactrocera cucurbitae (Coquillett), four populations of Bactrocera dorsalis (Hendel), Bactrocera zonata (Saunders), and Bactrocera tryoni (Froggatt), eggs (in vitro), and larvae (in infested fruit or on carrot diet) were cold treated at 2.0 +/- 0.2 degrees C for selected durations. The study was performed to assess whether a single (i.e., generic) cold treatment could be developed that would control the entire group of fruit flies that were tested. Probit regression models showed that the hierarchy of cold resistance was third-instar larvae reared on carrot diet> third-instar larvae reared on orange> eggs test in vitro. Differences in mortality responses of third-instar larvae reared in oranges across populations of B. dorsalis were observed only at subefficacious levels of control. The majority of Bactrocera species responded the same at the high levels of control demanded of phytosanitary treatments, which indicated that cold treatments would be similarly effective across the species and populations tested. B. cucurbitae was found to be the most cold tolerant of all the species tested. C1 [Myers, Scott W.] USDA, APHIS, PPQ, Ctr Plant Hlth Sci & Technol,Otis Lab, 1398 W Truck Rd, Buzzards Bay, MA 02542 USA. [Cancio-Martinez, Elena; Hallman, Guy J.; Fontenot, Emily A.; Vreysen, Marc J. B.] IAEA, Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Lab, Wagramerstr 5, A-1400 Vienna, Austria. RP Myers, SW (reprint author), USDA, APHIS, PPQ, Ctr Plant Hlth Sci & Technol,Otis Lab, 1398 W Truck Rd, Buzzards Bay, MA 02542 USA. EM scott.w.myers@aphis.usda.gov; E.I.Cancio-Martinez@iaea.org; G.J.Hallman@iaea.org; eagnesfontenot@gmail.com; M.Vreysen@iaea.org FU USDA Farm Bill Program FX We thank Mike Hennessey and Patrick Gomes, USDA-APHIS, for their support and contributions to this work. We thank Ulysses Sto. Tomas and Thilakasiri Dammalage of the IPCL for their technical support and assistance with colony maintenance. John W. Armstrong, Quarantine Scientific, Ltd., Kerikeri, New Zealand, provided a thorough and helpful review of an earlier version of this manuscript. This project was made possible through a USDA/IAEA joint project supported by the USDA Farm Bill Program. NR 28 TC 1 Z9 1 U1 6 U2 6 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD DEC PY 2016 VL 109 IS 6 BP 2341 EP 2347 DI 10.1093/jee/tow206 PG 7 WC Entomology SC Entomology GA EF4WU UT WOS:000390333500012 ER PT J AU Follett, PA Porcel, S Calcaterra, LA AF Follett, Peter A. Porcel, Sol Calcaterra, Luis A. TI Effect of Irradiation on Queen Survivorship and Reproduction in the Invasive Fire Ant Solenopsis invicta (Hymenoptera: Formicidae) and a Proposed Phytosanitary Irradiation Treatment for Ants SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE red imported fire ant; invasive; quarantine; phytosanitary treatment; generic irradiation dose ID CURCULIONIDAE; COLEOPTERA; LEPIDOPTERA; IMPACT; RATES AB We studied radiation tolerance in queens of the red imported fire ant Solenopsis invicta Buren (Hymenoptera: Formicidae) to identify a dose that prevents reproduction. Virgin or fertile queens were collected from Santa Fe and Formosa provinces in Argentina and reared in the laboratory in microcolonies. Virgin queens were irradiated at 0 (control), 70, 90, 120, or 150 Gy, and fertile queens were irradiated at 0, 60, 125, and 190 Gy, and then followed for 11 wk in the microcolonies to evaluate survival and reproduction. Virgin queens lay trophic eggs that do not hatch, whereas fertile queens lay eggs that hatch and develop into brood. In general, queen oviposition and survival decreased with increasing irradiation dose. For virgin queens, no eggs were laid by irradiated queens after the third week, whereas the control queens continued laying eggs throughout the 11-wk experiment. For fertile queens, only one larva and no pupae was observed in the 60 Gy treatment and no larvae or pupae were observed in the 125 and 190 Gy treatments, whereas a total of 431 larvae and 83 pupae were produced by untreated control queens during 11 wks. Survivorship of virgin and fertile queens was similarly reduced by irradiation treatment. These results with S. invicta are consistent with previous findings for three other invasive ants, Wasmannia auropunctata (Roger), Pheidole megacephala (F.), and Linephithema humile (Mayr), that are hitchhiker pests on fresh horticultural commodities. A radiation dose of 150 Gy is proposed as a phytosanitary treatment to prevent reproduction in ants. C1 [Follett, Peter A.] USDA ARS, US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. [Porcel, Sol; Calcaterra, Luis A.] Fdn Estudio Especies Invas, Hurlingham B1686EFA, Buenos Aires, DF, Argentina. [Porcel, Sol; Calcaterra, Luis A.] Consejo Nacl Invest Cient & Tecn, Hurlingham B1686EFA, Buenos Aires, DF, Argentina. RP Follett, PA (reprint author), USDA ARS, US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. EM peter.follett@ars.usda.gov; mariasolporcel@gmail.com; luiscalcaterra@hotmail.com FU United States Department of Agriculture, Foreign Agricultural Service, Technical Assistance for Specialty Crops (TASC) program FX We thank Sanford Porter and Nick Manoukis for their comments and suggestions to improve the manuscript, Eva Pawlak (Comision Nacional de Energia Atomica, Centro Atomico Ezeiza, Argentina) for irradiating the queens used in the experiments, and Nadia Jimenez and Andres Sanchez Restrepo for their technical assistance. The research was supported in part by a grant from the United States Department of Agriculture, Foreign Agricultural Service, Technical Assistance for Specialty Crops (TASC) program. NR 31 TC 0 Z9 0 U1 4 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD DEC PY 2016 VL 109 IS 6 BP 2348 EP 2354 DI 10.1093/jee/tow207 PG 7 WC Entomology SC Entomology GA EF4WU UT WOS:000390333500013 ER PT J AU Lujo, S Hartman, E Norton, K Pregmon, EA Rohde, BB Mankin, RW AF Lujo, S. Hartman, E. Norton, K. Pregmon, E. A. Rohde, B. B. Mankin, R. W. TI Disrupting Mating Behavior of Diaphorina citri (Liviidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE vibration; communication; huanglongbing; competitive disruption; noncompetitive disruption ID KUWAYAMA HEMIPTERA PSYLLIDAE; ASIAN CITRUS; INSECTICIDE RESISTANCE; HUANGLONGBING DISEASE; VIBRATIONAL SIGNALS; LEAFHOPPER; MODELS; COMMUNICATION; TECHNOLOGY; SYSTEM AB Severe economic damage from citrus greening disease, caused by `Candidatus Liberibacter asiaticus' bacteria, has stimulated development of methods to reduce mating and reproduction in populations of its insect vector, Diaphorina citri (Hemiptera: Liviidae). Male D. citri find mating partners by walking on host plants, intermittently producing vibrational calls that stimulate duetting replies by receptive females. The replies provide orientational feedback, assisting the search process. To test a hypothesis that D. citri mating can be disrupted using vibrational signals that compete with and/or mask female replies, courtship bioassays were conducted in citrus trees with or without interference from female reply mimics produced by a vibrating buzzer. Statistically significant reductions occurred in the rates and proportions of mating when the buzzer produced reply mimics within 0.4 s after male courtship calls compared with undisturbed controls. Observations of courtship behaviors in the two bioassays revealed activity patterns that likely contributed to the reductions. In both disruption and control tests, males reciprocated frequently between structural bifurcations and other transition points where signal amplitudes changed. Males in the disruption bioassay had to select among vibrational signals combined from the buzzer and the female at each transition point. They often turned towards the buzzer instead of the female. There was a statistically significant reduction in the proportion of males mating if they contacted the buzzer, possibly due to its higher vibration amplitude and duration in comparison with female replies. Potential applications of D. citri mating disruption technology in citrus groves are discussed. C1 [Lujo, S.; Hartman, E.; Norton, K.; Pregmon, E. A.; Mankin, R. W.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Rohde, B. B.] Univ Florida, Dept Elect & Comp Engn, Gainesville, FL 32611 USA. RP Mankin, RW (reprint author), ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. EM slujo@cfl.rr.com; hartmanethan@ufl.edu; knorton208@ufl.edu; epregmon@ufl.edu; barukh94-work@yahoo.com; Richard.Mankin@ars.usda.gov FU Florida Citrus Research and Development Fund; National Science Foundation [DGE-1315138] FX We thank Betty Weaver, Heidi Burnside, Nina Zagvazdina, and Everett Foreman (USDA-ARS-CMAVE), and Mary Dixon and Daniel Shahar (University of Florida) for rearing assistance and technical consultation. Funds for this research were provided by the Florida Citrus Research and Development Fund and National Science Foundation Graduate Research Fellowship DGE-1315138. Mention of a trademark or proprietary product is solely for the purpose of providing specific information and does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products that may also be suitable. The USDA is an equal opportunity employer. NR 50 TC 0 Z9 0 U1 8 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD DEC PY 2016 VL 109 IS 6 BP 2373 EP 2379 DI 10.1093/jee/tow202 PG 7 WC Entomology SC Entomology GA EF4WU UT WOS:000390333500016 ER PT J AU Spurgeon, DW AF Spurgeon, Dale W. TI Captures of Boll Weevils (Coleoptera: Curculionidae) in Relation to Trap Distance From Cotton Fields SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE boll weevil; Anthonomus grandis grandis; pheromone; trapping ID PHEROMONE TRAPS; ANTHONOMUS-GRANDIS; CENTRAL TEXAS; BRUSH LINES; POPULATIONS; EMERGENCE; PATTERNS; BEHAVIOR; SEASON AB The boll weevil (Anthonomus grandis grandis Boheman) has been eradicated from much of the United States, but remains an important pest of cotton (Gossypium spp.) in other parts of the Americas. Where the weevil occurs, the pheromone trap is a key tool for population monitoring or detection. Traditional monitoring programs have placed traps in or near the outermost cotton rows where damage by farm equipment can cause loss of trapping data. Recently, some programs have adopted a trap placement adjacent to but outside monitored fields. The effects of these changes have not been previously reported. Captures of early-season boll weevils by traps near (<= 1 m) or far (7-10 m) from the outermost cotton row were evaluated. In 2005, during renewed efforts to eradicate the boll weevil from the Lower Rio Grande Valley of Texas, far traps consistently captured more weevils than traps near cotton. Traps at both placements indicated similar patterns of early-season weevil captures, which were consistent with those previously reported. In 2006, no distinction between trap placements was detected. Early-season patterns of captures in 2006 were again similar for both trap placements, but captures were much lower and less regular compared with those observed in 2005. These results suggest magnitude and likelihood of weevil capture in traps placed away from cotton are at least as high as for traps adjacent to cotton. Therefore, relocation of traps away from the outer rows of cotton should not negatively impact ability to monitor or detect the boll weevil. C1 [Spurgeon, Dale W.] ARS, USDA, Arid Land Agr Res Ctr, 21881 N Cardon Lane, Maricopa, AZ 85138 USA. RP Spurgeon, DW (reprint author), ARS, USDA, Arid Land Agr Res Ctr, 21881 N Cardon Lane, Maricopa, AZ 85138 USA. EM dale.spurgeon@ars.usda.gov NR 36 TC 0 Z9 0 U1 1 U2 1 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD DEC PY 2016 VL 109 IS 6 BP 2405 EP 2410 DI 10.1093/jee/tow205 PG 6 WC Entomology SC Entomology GA EF4WU UT WOS:000390333500020 ER PT J AU Brookes, HM Ross, DW Strand, TM Thistle, HW Ragenovich, IR Lowrey, LL AF Brookes, Harrison M. Ross, Darrell W. Strand, Tara M. Thistle, Harold W. Ragenovich, Iral R. Lowrey, Laura L. TI Evaluating High Release RateMCH (3-Methylcyclohex-2-en-1-one) Treatments for Reducing Dendroctonus pseudotsugae (Coleoptera: Curculionidae) Infestations SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Douglas-fir beetle; Scolytinae; antiaggregation pheromone ID DOUGLAS-FIR BEETLE; HIGH-RISK STANDS; ANTIAGGREGATION PHEROMONE; SCOLYTIDAE; DISPENSERS; HOPKINS; ATTACK AB Current recommendations for applying the antiaggregation pheromone 3-methylcyclohex-2-en-1-one (MCH) to protect live trees from Douglas-fir beetle, Dendroctonus pseudotsugae Hopkins, infestation are to space individual passive releasers (MCH bubble capsules) on a 12- by 12-m grid throughout areas to be protected. Previous field studies and a theoretical study using a puff dispersion model to predict pheromone concentrations have shown that releasers emitting higher rates of MCH spaced farther apart may be as effective as the established standard treatment. During 2012 and 2013, we tested higher release rates of MCH at correspondingly wider spacings to keep the total amount of MCH released per unit area equal in all treatments. In 2012 near Challis, ID, treatments included the established standard release rate and spacing, four and six times the standard release rate at correspondingly wider spacings, and an untreated control. In 2013 near Ketchum, ID, treatments included the established standard release rate and spacing, five and seven times the standard release rate at correspondingly wider spacings, and an untreated control. Results from both years indicated that all MCH treatments were equally effective in reducing Douglas-fir beetle infestation. Using higher release rate formulations at wider spacings will reduce labor costs of installing MCH treatments, and, in cases where it is necessary, retrieving the releasers as well. In addition to reducing labor costs, the revised treatment protocol may increase the feasibility of treating areas that currently may not be possible due to treatment costs. C1 [Brookes, Harrison M.; Ross, Darrell W.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Strand, Tara M.] Scion, NZ Forest Res Inst, Forest Protect, 49 Sala St, Rotorua 3046, New Zealand. [Thistle, Harold W.] US Forest Serv, Forest Hlth Technol Enterprise Team, USDA, 180 Canfield St, Morgantown, WV 26505 USA. [Ragenovich, Iral R.] US Forest Serv, Forest Hlth Protect, USDA, 1220 SW Third Ave, Portland, OR 97208 USA. [Lowrey, Laura L.] US Forest Serv, Forest Hlth Protect, USDA, 1249 S Vinnell Way, Boise, ID 83709 USA. RP Ross, DW (reprint author), Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. EM brookeha@onid.oregonstate.edu; darrell.ross@oregonstate.edu; Tara.Strand@scionresearch.com; hthistle@fs.fed.us; iragenovich@fs.fed.us; laurallowrey@fs.fed.us FU USDA Forest Service, Forest Health Protection Special Technology Development Program FX We thank the personnel of the Salmon-Challis and Sawtooth National Forests for allowing us to conduct the research and aiding in the collection of insect trap samples. Additional thanks to Daniel Ott, Juan Pajares, and Philip Mocettini for their help in setting up and evaluating the research plots. Funding for this project was provided by the USDA Forest Service, Forest Health Protection Special Technology Development Program. NR 20 TC 0 Z9 0 U1 1 U2 1 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD DEC PY 2016 VL 109 IS 6 BP 2424 EP 2427 DI 10.1093/jee/tow208 PG 4 WC Entomology SC Entomology GA EF4WU UT WOS:000390333500023 ER PT J AU Kendra, PE Montgomery, WS Schnell, EQ Deyrup, MA Epsky, ND AF Kendra, Paul E. Montgomery, Wayne S. Schnell, Elena Q. Deyrup, Mark A. Epsky, Nancy D. TI Efficacy of alpha-Copaene, Cubeb, and Eucalyptol Lures for Detection of Redbay Ambrosia Beetle (Coleoptera: Curculionidae: Scolytinae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Xyleborus glabratus; laurel wilt; kairomone; sesquiterpene; Lauraceae ID SOUTHEASTERN UNITED-STATES; XYLEBORUS-GLABRATUS; LAUREL WILT; OIL LURES; ELECTROANTENNOGRAM RESPONSES; RAFFAELEA-LAURICOLA; TERPENOID EMISSIONS; MANUKA OIL; PHOEBE OIL; ATTRACTION AB Redbay ambrosia beetle, Xyleborus glabratus Eichhoff, is a wood-boring pest that has now invaded nine states in the southeastern United States. The beetle's dominant fungal symbiont (Raffaelea lauricola) is phytopathogenic, inducing laurel wilt in trees within the family Lauraceae. Members of the genus Persea are particularly susceptible to the lethal disease, including native redbay (P. borbonia) and swampbay (P. palustris), as well as commercial avocado (P. americana). Cubeb oil lures are the current standard for detection of X. glabratus, but recently eucalyptol and a 50% alpha-copaene oil have been identified as additional attractants. This study used a combination of binary-choice bioassays, field cage release-and-recapture assays, and a 12-wk field trial to compare efficacy of eucalyptol and copaene lures relative to commercial cubeb lures. In addition, GC-MS was used to quantify emissions from lures field-aged for 12 wk. In laboratory bioassays, copaene lures were more attractive than eucalyptol lures. In field cage assays, copaene lures recaptured a higher percentage of released beetles than cubeb lures. In the field test, cubeb lures captured fewer beetles than copaene lures, and lowest captures were obtained with eucalyptol lures. Combining eucalyptol with either copaene or cubeb lures did not increase captures over those lures deployed alone. Both copaene and cubeb lures were effective in attracting X. glabratus for 12 wk, but field life of eucalyptol lures was only 4 wk, consistent with the quantification of lure emissions. Results suggest that the 50% a-copaene lure provides the best pest detection currently available for X. glabratus. C1 [Kendra, Paul E.; Montgomery, Wayne S.; Schnell, Elena Q.; Epsky, Nancy D.] USDA ARS, Subtrop Hort Res Stn, 13601 Old Cutler Rd, Miami, FL 33158 USA. [Deyrup, Mark A.] Archbold Biol Stn, POB 2057, Lake Placid, FL 33862 USA. RP Kendra, PE (reprint author), USDA ARS, Subtrop Hort Res Stn, 13601 Old Cutler Rd, Miami, FL 33158 USA. EM paul.kendra@ars.usda.gov; wayne.montgomery@ars.usda.gov; elena.schnell@ars.usda.gov; mdeyrup@archbold-station.org; Nancy.Epsky@ars.usda.gov FU USDA-ARS National Plant Disease Recovery System; Florida Avocado Administrative Committee FX We are grateful to Hilary Swain for providing laboratory space and lodging at Archbold Biological Station (Lake Placid, FL), to John Smoak III for granting permission to conduct field trials on his property, and to Larissa Guillen (Instituto de Ecologia; Xalapa, Veracruz, Mexico) and David Owens (USDA-ARS; Miami, FL) for providing reviews of an earlier version of the manuscript. We also acknowledge Synergy Semiochemicals Corp. (Burnaby, BC, Canada) for providing material used in laboratory and field evaluations. This work was supported, in part, by funding from the USDA-ARS National Plant Disease Recovery System and the Florida Avocado Administrative Committee. Mention of a proprietary product does not constitute an endorsement by the USDA-ARS. NR 39 TC 1 Z9 1 U1 1 U2 1 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD DEC PY 2016 VL 109 IS 6 BP 2428 EP 2435 DI 10.1093/jee/tow214 PG 8 WC Entomology SC Entomology GA EF4WU UT WOS:000390333500024 PM 27986939 ER PT J AU Coleman, TW Smith, SL Jones, MI Graves, AD Strom, BL AF Coleman, Tom W. Smith, Sheri L. Jones, Michael I. Graves, Andrew D. Strom, Brian L. TI Effect of Contact Insecticides Against the Invasive Goldspotted Oak Borer (Coleoptera: Buprestidae) in California SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE bifenthrin; carbaryl; exotic; oak mortality; Quercus spp ID AGRILUS-PLANIPENNIS COLEOPTERA; BARK BEETLE ATTACK; SOUTHERN CALIFORNIA; UNITED-STATES; COXALIS WATERHOUSE; AUROGUTTATUS; QUERCUS; FOREST; WOODLANDS AB The goldspotted oak borer, Agrilus auroguttatus Schaeffer (Coleoptera: Buprestidae), was linked in 2008 to ongoing tree mortality in oak woodlands of southern California. Mortality of coast live oak, Quercus agrifolia Nee, and California black oak, Q. kelloggii Newb., continues as this exotic phloem borer spreads in southern California. Management options are needed to preserve high-value oaks and maintain management objectives. From 2009 to 2012, we tested four contact insecticide formulations in four experiments against A. auroguttatus in California. The impact of contact insecticides was evaluated similar to<1, 8, and 12 mo postapplication against A. auroguttatus adults in no-choice leaf-feeding or walking bioassays. At <1 mo postapplication, bifenthrin, carbaryl, lambda-cyhalothrin, and permethrin all reduced adult survival and feeding in leaf-feeding and walking bioassays. At 8 mo postapplication, only bifenthrin reduced adult feeding, but had no effect on survivorship. At 12 mo postapplication, adult A. auroguttatus survived fewer days and fed less in leaf-feeding bioassays with bifenthrin, carbaryl, and permerthin. These results support the annual application of contact insecticides prior to A. auroguttatus' flight period to reduce adult leaf maturation feeding and activity on the bark surface (e.g., oviposition), but additional studies are needed to show these contact treatments can prevent tree mortality from this invasive species. C1 [Coleman, Tom W.] US Forest Serv, USDA, Forest Hlth Protect, 602 S Tippecanoe Ave, San Bernardino, CA 92408 USA. [Smith, Sheri L.] US Forest Serv, USDA, Forest Hlth Protect, 2550 Riverside Dr, Susanville, CA 96130 USA. [Jones, Michael I.] Univ Calif Davis, Dept Entomol & Nematol, Briggs Hall, Davis, CA 95616 USA. [Jones, Michael I.] SUNY Coll Environm Sci & Forestry, Dept Environm & Forest Biol, Syracuse, NY 13210 USA. [Graves, Andrew D.] US Forest Serv, USDA, Forest Hlth Protect, 333 Broadway Blvd SE, Albuquerque, NM 87102 USA. [Strom, Brian L.] US Forest Serv, USDA, Southern Res Stn, 2500 Shreveport Highway, Pineville, LA 71360 USA. RP Coleman, TW (reprint author), US Forest Serv, USDA, Forest Hlth Protect, 602 S Tippecanoe Ave, San Bernardino, CA 92408 USA. EM twcoleman@fs.fed.us; ssmith11@fs.fed.us; mijone01@syr.edu; adgraves@fs.fed.us; brianstrom@fs.fed.us FU USDA Forest Service, Forest Service Pesticide Application Impact Program; USDA Forest Service, Forest Health Protection, Region 3; USDA Forest Service Research, Southern Research Station; Department of Entomology and Nematology, University of California, Davis; Cleveland National Forest, Descanso Ranger District; USDA Forest Service, Forest Health Protection, Region 5 FX We thank Mary Louise Flint, Stacy Hishinuma, and Adrian Poloni, Department of Entomology and Nematology, University of California, Davis; Daniel Betancourt, Andreana Cipollone, Kalee Koeslag, Jonathan Mireles, and Grayland Walter, USDA Forest Service, Forest Health Protection, Region 5; Steven J. Seybold, USDA Forest Service, Pacific Southwest Research Station, Davis, CA, and the Cleveland National Forest, Descanso Ranger District for their support of this work. We thank two anonymous reviewers for critical reviews of an earlier version of the manuscript. Funding for this work was provided by the USDA Forest Service, Forest Service Pesticide Application Impact Program; USDA Forest Service, Forest Health Protection, Regions 3 and 5; USDA Forest Service Research, Southern Research Station; and Department of Entomology and Nematology, University of California, Davis. NR 36 TC 0 Z9 0 U1 3 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD DEC PY 2016 VL 109 IS 6 BP 2436 EP 2445 DI 10.1093/jee/tow217 PG 10 WC Entomology SC Entomology GA EF4WU UT WOS:000390333500025 PM 27986940 ER PT J AU Caprio, MA Edwards, KT Musser, FR Allen, KC Fleming, DE AF Caprio, M. A. Edwards, K. T. Musser, F. R. Allen, K. C. Fleming, D. E. TI Impact of Insect Management on Population Dynamics and Insecticide Resistance of Tarnished Plant Bug (Hemiptera: Miridae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE cotton insects; insecticide resistance; population dynamics; population ecology; population modeling ID LYGUS-LINEOLARIS HETEROPTERA; AMARANTH AMARANTHUS-PALMERI; MISSISSIPPI RIVER DELTA; HOST PLANTS; REPRODUCTIVE STATUS; FLOWERING COTTON; UNITED-STATES; ARKANSAS; GENE; THRESHOLDS AB While transgenic plants targeting lepidopteran and coleopteran insects have been available for almost 20 yr, there are no transgenic crops that target hemipteran insects such as tarnished plant bug, Lygus lineolaris (Palisot de Beauvois), though at least one company lists potential products in advanced stages of development. A resistance management model for the U.S. Mid-South was developed to aid in resistance risk assessments for transgenic crops targeting L. lineolaris, and validated against the prior case of pyrethroid resistance in this insect. The model predicted that resistance to a pyrethroid would evolve in 17.6-21.0 yr (depending on the initial resistance allele frequency), which was close to the 15-20 years observed in the field. Due to uncertainty in female fecundity, we varied fecundity from 3 eggs/female/day to 12 eggs/female/day. Sprays were applied based on action thresholds, and increasing fecundity therefore increased the number of sprays applied per year and decreased durability. We also varied the action threshold in cotton (Gossypium hirsutum L.) fields (the population levels at which sprays were applied) from 17,600/ha to as low as 1,100/ha. Lowering the threshold increased the number of sprays applied, but also increased durability of the pesticide. Removing the noncotton host refuge present at the same time as cotton changed the relationship so that increasing the action threshold increased durability. The impact of insect resistance management on action threshold cost estimates will vary depending on the landscape, and cannot be assumed to always move the economic injury level in the same direction. C1 [Caprio, M. A.; Edwards, K. T.; Musser, F. R.; Fleming, D. E.] Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Box 9775, Mississippi State, MS 39762 USA. [Allen, K. C.] USDA ARS, Southern Insect Management Res Unit, Box 346, Stoneville, MS 38776 USA. RP Caprio, MA (reprint author), Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Box 9775, Mississippi State, MS 39762 USA. EM mcaprio@entomology.msstate.edu; kt20@msstate.edu; fm61@msstate.edu; Clint.Allen@ARS.USDA.GOV; def18@msstate.edu FU Cotton Foundation; Cotton Incorporated FX We would like to thank Ryan Kurtz and two anonymous reviewers for their very helpful reviews of an earlier version of this manuscript. This research was supported by the Cotton Foundation and Cotton Incorporated. This publication is a contribution of the Mississippi Agricultural and Forestry Experiment Station. NR 52 TC 0 Z9 0 U1 6 U2 6 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD DEC PY 2016 VL 109 IS 6 BP 2517 EP 2524 DI 10.1093/jee/tow185 PG 8 WC Entomology SC Entomology GA EF4WU UT WOS:000390333500035 ER PT J AU DeBlasio, SL Johnson, RS MacCoss, MJ Gray, SM Cilia, M AF DeBlasio, Stacy L. Johnson, Richard S. MacCoss, Michael J. Gray, Stewart M. Cilia, Michelle TI Model System-Guided Protein Interaction Mapping for Virus Isolated from Phloem Tissue SO JOURNAL OF PROTEOME RESEARCH LA English DT Article DE Luteoviridae; polerovirus; Potato leafroll virus; host-pathogen interactions; phloem-limited; co-immunoprecipitation; high-resolution mass spectrometry; model system; Solanum tuberosum; SAINT ID POTATO-LEAFROLL-VIRUS; WESTERN YELLOWS VIRUS; PURIFICATION-MASS SPECTROMETRY; MAJOR CAPSID PROTEIN; APHID TRANSMISSION; READTHROUGH PROTEIN; POINT MUTATIONS; INTERACTION NETWORKS; VIRION STABILITY; ROLL VIRUS AB Phloem localization of plant viruses is advantageous for acquisition by sap sucking vectors but hampers host virus protein interaction studies. In this study, Potato leafroll virus (PLRV) host protein complexes were isolated from systemically infected potato, a natural host of the virus. Comparing two different co-immunoprecipitation (co-IP) support matrices coupled to mass spectrometry (MS), we identified 44 potato proteins and one viral protein (P1) specifically associated with virus isolated from infected phloem. An additional 142 proteins interact in complex with virus at varying degrees of confidence. Greater than 80% of these proteins were previously found to form high confidence interactions with PLRV isolated from the model host Nicotiana benthamiana. Bioinformatics revealed that these proteins are enriched for functions related to plasmodesmata, organelle membrane transport, translation, and mRNA processing. Our results show that model system proteomics experiments are extremely valuable for understanding protein interactions regulating infection in recalcitrant pathogens such as phloem-limited viruses. C1 [DeBlasio, Stacy L.; Gray, Stewart M.; Cilia, Michelle] USDA, Agr Res Serv, Ithaca, NY 14853 USA. [DeBlasio, Stacy L.; Cilia, Michelle] Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Johnson, Richard S.; MacCoss, Michael J.] Univ Washington, Dept Genome Sci, Seattle, WA 98109 USA. [Gray, Stewart M.; Cilia, Michelle] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. RP Cilia, M (reprint author), USDA, Agr Res Serv, Ithaca, NY 14853 USA.; Cilia, M (reprint author), Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA.; Cilia, M (reprint author), Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. EM mlc68@cornell.edu FU USDA NIFA Grant [1907-22000-021-20]; NSF Grant [1354309]; USDA Agricultural Research Service CRIS Project [8062-22410-006-00-D] FX The authors would like to acknowledge Katherine Parks, Rogerio Santos (Boyce Thompson Institute, BTI) and Merritt Compton (Cornell) for plant husbandry. We are appreciative of Dr. Todd Greco (Princeton) for feedback on methods and James VanEe and John Flaherty (Cornell Bio-IT) for IT support. We thank Kevin Howe for coordinating the data upload to ProteomeXchange. Funds for this research were provided by USDA NIFA Grant 1907-22000-021-20, NSF Grant 1354309, and USDA Agricultural Research Service CRIS Project 8062-22410-006-00-D. NR 69 TC 0 Z9 0 U1 5 U2 5 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1535-3893 EI 1535-3907 J9 J PROTEOME RES JI J. Proteome Res. PD DEC PY 2016 VL 15 IS 12 BP 4601 EP 4611 DI 10.1021/acs.jproteome.6b00715 PG 11 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA EE2EM UT WOS:000389396500037 PM 27762138 ER PT J AU Elias, E Rango, A Steele, CM Mejia, JF Baca, R James, D Schrader, S Gronemeyer, P AF Elias, Emile Rango, Albert Steele, Caitriana M. Mejia, John F. Baca, Ruben James, Darren Schrader, Scott Gronemeyer, Peg TI Simulated impact of climate change on hydrology of multiple watersheds using traditional and recommended snowmelt runoff model methodology SO JOURNAL OF WATER AND CLIMATE CHANGE LA English DT Article DE climate change; regional climate modeling; Rio Grande; snowmelt runoff model ID PRECIPITATION; VARIABILITY; STREAMFLOW; BASINS AB For more than two decades researchers have utilized the snowmelt runoff model (SRM) to test the impacts of climate change on streamflow of snow-fed systems. SRM developers recommend a parameter shift during simulations of future climate, but this is often omitted. Here we show the impact of this omission on model results. In this study, the hydrological effects of climate change are modeled over three sequential years with typical and recommended SRM methodology. We predict the impacts of climate change on water resources of five subbasins of an arid region. Climate data are downscaled to weather stations. Period change analysis gives temperature and precipitation changes for 55 general circulation models which are then subsampled to produce four future states per basin. Results indicate an increase in temperature between 3.0 and 6.2 degrees C and an 18% decrease to 26% increase in precipitation. Without modifications to the snow runoff coefficient (C-S), mean results across all basins range from a reduction in total volume of 21% to an increase of 4%. Modifications to C-S resulted in a 0-10% difference in simulated annual volume. Future application of SRM should include a parameter shift representing the changed climate. C1 [Elias, Emile; Rango, Albert; James, Darren; Schrader, Scott] ARS, USDA, Jornada Expt Range, 2995 Knox St, Las Cruces, NM 88033 USA. [Steele, Caitriana M.; Baca, Ruben; Gronemeyer, Peg] New Mexico State Univ, 2995 Knox St, Las Cruces, NM 88033 USA. [Mejia, John F.] Desert Res Inst, Dept Atmospher Sci, 2215 Raggio Pkwy, Reno, NV 89512 USA. RP Elias, E (reprint author), ARS, USDA, Jornada Expt Range, 2995 Knox St, Las Cruces, NM 88033 USA. EM eliaseh@nmsu.edu FU USBR WaterSmart program [R11Ap81455]; Desert Research Institute (DRI); National Science Foundation: New Mexico EPSCoR [814449] FX Downscaling information is based upon work supported by the USBR WaterSmart program (#R11Ap81455; Mejia) and the Desert Research Institute (DRI). We also acknowledge the modeling groups for making their model output available for analysis: the Program for Climate Model Diagnosis and Intercomparison (PCMDI) for collecting and archiving this data and the WCRP's Working Group on Coupled Modelling (WGCM) for organizing the model data analysis activity and BCCA for their downscaling efforts. Portions of this study were funded by the National Science Foundation grant #814449: New Mexico EPSCoR. We thank Jaroslav Martinec for his help in parameterising the basins simulated here and Ryann Smith for project support. NR 65 TC 0 Z9 0 U1 11 U2 11 PU IWA PUBLISHING PI LONDON PA ALLIANCE HOUSE, 12 CAXTON ST, LONDON SW1H0QS, ENGLAND SN 2040-2244 J9 J WATER CLIM CHANGE JI J. Water Clim. Chang. PD DEC PY 2016 VL 7 IS 4 BP 665 EP 682 DI 10.2166/wcc.2016.097 PG 18 WC Water Resources SC Water Resources GA EF6IH UT WOS:000390433900002 ER PT J AU Reinhart, KO Dangi, SR Vermeire, LT AF Reinhart, Kurt O. Dangi, Sadikshya R. Vermeire, Lance T. TI The effect of fire intensity, nutrients, soil microbes, and spatial distance on grassland productivity SO PLANT AND SOIL LA English DT Article DE Co-limitation; Ecosystemmanagement; Multiple regression; Rangeland; Semi-arid grassland ID COMMUNITY STRUCTURE; CONTEMPORARY PERSPECTIVE; NITROGEN LIMITATION; PRESCRIBED FIRE; PLANT COMMUNITY; ORGANIC-MATTER; SUMMER FIRE; RESPONSES; DYNAMICS; PRAIRIE AB Variation in fire intensity within an ecosystem is likely to moderate fire effects on plant and soil properties. We tested the effect of fire intensity on grassland biomass, soil microbial biomass, and soil nutrients. Additional tests determined plant-microbe, plant-nutrient, and microbe-nutrient associations. A replicated field experiment produced a fire intensity gradient. We measured plant and soil microbial biomasses at peak plant productivity the first growing season after fire. We concurrently measured flux in 11 soil nutrients and soil moisture. Fire intensity positively affected soil nitrogen, phosphorus (P), and zinc but did not appreciably affect plant biomass, microbial biomass, and other soil nutrients. Plant biomass was seemingly (co-)limited by boron, manganese, and P. Microbial biomass was (co-)limited mainly by P and also iron. In the Northern Great Plains, plant and soil microbial biomasses were limited mainly by P and some micronutrients. Fire intensity affected soil nutrients, however, pulsed P (due to fire) did not result in appreciable fire intensity effects on plant and microbial biomasses. Variable responses in plant productivity to fire are common and indicate the complexity of factors that regulate plant production after fire. C1 [Reinhart, Kurt O.; Vermeire, Lance T.] USDA ARS, Ft Keogh Livestock & Range Res Lab, 243 Ft Keogh Rd, Miles City, MT 59301 USA. [Dangi, Sadikshya R.] USDA ARS, Water Management Res Unit, San Joaquin Valley Agr Sci Ctr, 9611 S Riverbend Ave, Parlier, CA 93648 USA. RP Reinhart, KO (reprint author), USDA ARS, Ft Keogh Livestock & Range Res Lab, 243 Ft Keogh Rd, Miles City, MT 59301 USA. EM kurt.reinhart@ars.usda.gov FU USDA appropriated funds (CRIS) [5434-21630-003-00D] FX We thank D. Strong, A. Roth, P. Smith, M. Russell, C. Murphy, D. Correia, A. Marquez, M. Rout, and numerous summer interns and students between 2006 and 2013 for assistance in the field and laboratory. We thank D. Augustine and two anonymous reviewers for comments on an earlier version of the manuscript. This work was funded by USDA appropriated funds (CRIS # 5434-21630-003-00D). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 80 TC 1 Z9 1 U1 26 U2 26 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X EI 1573-5036 J9 PLANT SOIL JI Plant Soil PD DEC PY 2016 VL 409 IS 1-2 BP 203 EP 216 DI 10.1007/s11104-016-2957-3 PG 14 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA EF0RZ UT WOS:000390034900014 ER PT J AU Essarioui, A Kistler, HC Kinkel, LL AF Essarioui, Adil Kistler, Harold C. Kinkel, Linda L. TI Nutrient use preferences among soil Streptomyces suggest greater resource competition in monoculture than polyculture plant communities SO PLANT AND SOIL LA English DT Article DE Streptomyces; Andropogon gerardii; Lespedeza capitata; Plant richness ID BIODIVERSITY; GROWTH; GRASSLAND; DIVERSITY; DYNAMICS; DISEASES AB Nutrient use overlap among sympatric Streptomyces populations is correlated with pathogen inhibitory capacity, yet there is little information on either the factors that influence nutrient use overlap among coexisting populations or the diversity of nutrient use among soil Streptomyces. We examined the effects of plant host and plant species richness on nutrient use of Streptomyces isolated from the rhizosphere of Andropogon gerardii (Ag) and Lespedeza capitata (Lc) growing in communities of 1 (monoculture) or 16 (polyculture) plant species. Growth on 95 carbon sources was assessed over 5d. Cumulative growth was significantly greater for polyculture vs. monoculture isolates, and for Lc vs. Ag isolates. Isolates from monocultures, but not polycultures, exhibited a drop in growth rates between 24 h and 72 h post-inoculation, suggesting resource allocation to non-growth functions. Isolates from high-carbon (polyculture) or high-nitrogen (Lc) soils had larger niche widths than isolates from low-C (monocultures) or low-N (Ag) soils. Sympatric isolates from polycultures were significantly more differentiated from one another in preferred nutrients for growth than sympatric isolates from monocultures. These results suggest that Streptomyces populations respond to selection imposed by plant host and plant community richness and that populations from polyculture but not from monoculture, mediate resource competition via niche differentiation. C1 [Essarioui, Adil; Kistler, Harold C.; Kinkel, Linda L.] Univ Minnesota, Dept Plant Pathol, 491 Borlaug Hall,1991 Upper Buford Circle, St Paul, MN 55108 USA. [Essarioui, Adil] Natl Inst Agron Res, Reg Ctr Errachidia, Ave Marche Verte, Errachidia, Morocco. [Kistler, Harold C.] USDA ARS, Cereal Dis Lab, 1551 Lindig Ave, St Paul, MN 55108 USA. RP Essarioui, A (reprint author), Natl Inst Agron Res, Reg Ctr Errachidia, Ave Marche Verte, Errachidia, Morocco. EM essar002@umn.edu FU Islamic Development Bank; Agricultural and Food Research Grant Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2011-67019-30200]; National Science Foundation [0620652] FX Adil Essarioui was supported by funds from the Islamic Development Bank. Research was supported by Agricultural and Food Research Grant Initiative Competitive Grant 2011-67019-30200 from the USDA National Institute of Food and Agriculture. Technical and field support from Lindsey Hanson, Dan Schlatter, and Nick LeBlanc were invaluable to completion of the work. Field plots maintained under National Science Foundation Long-Term Ecological Research Grant 0620652 were the source of soil samples used in this study. NR 38 TC 0 Z9 0 U1 9 U2 9 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X EI 1573-5036 J9 PLANT SOIL JI Plant Soil PD DEC PY 2016 VL 409 IS 1-2 BP 329 EP 343 DI 10.1007/s11104-016-2968-0 PG 15 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA EF0RZ UT WOS:000390034900023 ER PT J AU Polley, HW Collins, HP Reichmann, LG Fay, PA AF Polley, H. Wayne Collins, Harold P. Reichmann, Lara G. Fay, Philip A. TI CO2 and soil water potential as regulators of the growth and N fraction derived from fixation of a legume in tallgrass prairie communities SO PLANT AND SOIL LA English DT Article DE delta N-15 natural abundance; Desmanthus illinoensis; Essential elements; Soil texture; Soil water potential; Subambient CO2 ID PLANT-SPECIES COMPOSITION; ELEVATED ATMOSPHERIC CO2; SYMBIOTIC N-2 FIXATION; NITROGEN-FIXATION; CARBON-DIOXIDE; GRASSLAND PRODUCTIVITY; CALCAREOUS GRASSLAND; RESPONSES; ENRICHMENT; MINERALIZATION AB CO2 enrichment may increase N input to ecosystems by increasing N-2 fixation, but the fixation-CO2 response depends on factors such as soil water availability that are influenced by soil properties. We used the delta N-15 natural abundance method to estimate the proportion of N in the legume Desmanthus illinoensis that was derived from N-2 fixation following 2-8 years of growth along a subambient to elevated CO2 gradient. Desmanthus was grown in tallgrass prairie communities on each of three soils of differing texture. Only on a clay soil was it possible to calculate fixation (N-fix; g N m(-2)). The fraction of legume N derived from fixation (N-dfa) decreased by 20 % as CO2 increased from subambient to elevated concentrations. The negative effect of reduced N-dfa on N-fix was obscured by variation in Desmanthus production along the CO2 gradient that was positively linked to the ANPP-CO2 response of communities. Across soils, legume production was negatively correlated with soil water potential to 0.3 m depth (I(soil)). N-fix in grasslands may depend primarily on I(soil) as influenced by soil hydrological properties. CO2 enrichment may reduce N-fix during years in which the legume-CO2 and related ANPP-CO2 response is small by depressing N-dfa. C1 [Polley, H. Wayne; Collins, Harold P.; Reichmann, Lara G.; Fay, Philip A.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. RP Polley, HW (reprint author), USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. EM wayne.polley@ars.usda.gov NR 48 TC 0 Z9 0 U1 11 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X EI 1573-5036 J9 PLANT SOIL JI Plant Soil PD DEC PY 2016 VL 409 IS 1-2 BP 361 EP 370 DI 10.1007/s11104-016-2971-5 PG 10 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA EF0RZ UT WOS:000390034900025 ER PT J AU Rodriguez-Rivera, LD Cummings, KJ McNeely, I Suchodolski, JS Scorza, AV Lappin, MR Mesenbrink, BT Leland, BR Bodenchuk, MJ AF Rodriguez-Rivera, Lorraine D. Cummings, Kevin J. McNeely, Isaac Suchodolski, Jan S. Scorza, Andrea V. Lappin, Michael R. Mesenbrink, Brian T. Leland, Bruce R. Bodenchuk, Michael J. TI Prevalence and Diversity of Cryptosporidium and Giardia Identified Among Feral Pigs in Texas SO VECTOR-BORNE AND ZOONOTIC DISEASES LA English DT Article DE Cryptosporidium; epidemiology; feral pigs; Giardia; surveillance; zoonotic ID UNITED-STATES; SUS-SCROFA; SP APICOMPLEXA; RISK-FACTORS; CATTLE; SUIS; SWINE; EPIDEMIOLOGY; SURVEILLANCE; CALIFORNIA AB The population size and geographic range of feral pigs in the United States are rapidly expanding. Nevertheless, the role of this invasive species in the ecology and transmission of zoonotic enteric pathogens is poorly understood. Our objectives were to describe the prevalence and diversity of Cryptosporidium and Giardia shedding among feral pigs throughout Texas and to identify risk factors for infection. Fecal samples were collected from feral pigs in Texas from February 2014 through May 2015. Cryptosporidium oocysts and Giardia cysts were detected using a direct immunofluorescence assay, and genotyping of positive samples was performed. The prevalence of Cryptosporidium shedding was 1.6% (6/370), and C. scrofarum and C. suis were identified. The prevalence of Giardia shedding was 4.3% (16/370), and assemblages A and E were identified. Cryptosporidium shedding was significantly more common among juvenile and subadult pigs than among adult pigs, but age group was not associated with Giardia shedding status. Feral pigs may serve as a source of Cryptosporidium and Giardia transmission to humans and livestock. C1 [Rodriguez-Rivera, Lorraine D.; Cummings, Kevin J.] Texas A&M Univ, Dept Vet Integrat Biosci, College Stn, TX USA. [McNeely, Isaac; Suchodolski, Jan S.] Texas A&M Univ, Dept Small Anim Clin Sci, College Stn, TX USA. [Scorza, Andrea V.; Lappin, Michael R.] Colorado State Univ, Dept Clin Sci, Coll Vet Med & Biomed Sci, Ft Collins, CO 80523 USA. [Mesenbrink, Brian T.; Leland, Bruce R.; Bodenchuk, Michael J.] USDA, Wildlife Serv, Anim & Plant Hlth Inspect Serv, San Antonio, TX USA. RP Cummings, KJ (reprint author), Texas A&M Univ, Dept Vet Integrat Biosci, Coll Vet Med & Biomed Sci, College Stn, TX 77843 USA. EM kcummings@cvm.tamu.edu FU USDA National Institute of Food and Agriculture Formula Animal Health Fund FX We thank all participating wildlife biologists who submitted samples over the duration of the study. This research was supported by the USDA National Institute of Food and Agriculture Formula Animal Health Fund. NR 26 TC 0 Z9 0 U1 4 U2 4 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1530-3667 EI 1557-7759 J9 VECTOR-BORNE ZOONOT JI Vector-Borne Zoonotic Dis. PD DEC PY 2016 VL 16 IS 12 BP 765 EP 768 DI 10.1089/vbz.2016.2015 PG 4 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA EF3RX UT WOS:000390243200005 PM 27763822 ER PT J AU Liu, YL Wang, JW Wolcott, MP AF Liu, Yalan Wang, Jinwu Wolcott, Michael P. TI Multistep Process to Produce Fermentable Sugars and Lignosulfonates from Softwood Enzymolysis Residues SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Acid bisulfite treatment; Neutral sulfite treatment; Lignosulfonates; Sugars; Enzymatic hydrolysis ID ETHANOL-PRODUCTION; ENZYMATIC-HYDROLYSIS; LODGEPOLE-PINE; LIGNOCELLULOSIC BIOMASS; CORN STOVER; LIGNIN; PRETREATMENT; SPORL; HEMICELLULOSE; DIGESTIBILITY AB The residual solids from enzymatic hydrolysis are usually burned to produce energy and have been explored as a feedstock for various products including activated carbon and lignin based polymers. These products require additional procedures unrelated to the existing biorefinery equipment. In the current study, we proposed successive sulfite treatments to utilize the enzymolysis residues for producing fermentable sugars and lignosulfonates. Two sulfite methods were compared as an initial pretreatment. It was found that the acid bisulfite treatment achieved higher hemicellulose removal (90%), while neutral sulfite resulted in higher lignin removal (50%) and a greater lignosulfonate yield (26.6 g from 100 g raw material). Overall, the acid bisulfite treatment resulted in 66% glucan conversion by enzymatic hydrolysis, whereas it was only 37% for the neutral sulfite method. In addition, the neutral sulfite pretreated wood reached 80% of the maximum sugar yield after 6 h of enzymatic hydrolysis, whereas it was 48 h for the acid bisulfite pretreated wood. The multistep treatment process achieved 84% total sugar conversion and 58% lignin removal with 32.4 g lignosulfonates being produced from 100 g raw materials. The results demonstrate that the multistep treatment process has a potential to maximize the yields of fermentable sugars and lignosulfonates. C1 [Liu, Yalan; Wolcott, Michael P.] Washington State Univ, Composite Mat & Engn Ctr, 2001 East Grimes Way, Pullman, WA 99164 USA. [Wang, Jinwu] US Forest Serv, Forest Prod Lab, USDA, 35 Flagstaff Rd, Orono, ME 04469 USA. RP Liu, YL (reprint author), Washington State Univ, Composite Mat & Engn Ctr, 2001 East Grimes Way, Pullman, WA 99164 USA. EM yalan.liu@wsu.edu FU Agriculture and Food Research Initiative Competitive from the USDA National Institute of Food and Agriculture [2011-68005-30416]; China Scholarship Council FX The chemical liquor in this study was kindly provided and analyzed by Weyerhaeuser with help from Dr. Jonhway Gao. We gratefully acknowledge the Northwest Advanced Renewables Alliance (NARA), supported by the Agriculture and Food Research Initiative Competitive Grant no. 2011-68005-30416 from the USDA National Institute of Food and Agriculture, and the China Scholarship Council funding. NR 42 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD DEC PY 2016 VL 4 IS 12 BP 7225 EP 7230 DI 10.1021/acssuschemeng.6b02328 PG 6 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA EE3OC UT WOS:000389497900109 ER PT J AU Power, TG Olivera, YA Hill, RA Beck, AD Hopwood, V Garcia, KS Ramos, GG Fisher, JO O'Connor, TM Hughes, SO AF Power, Thomas G. Olivera, Yadira A. Hill, Rachael A. Beck, Ashley D. Hopwood, Veronica Garcia, Karina Silva Ramos, Guadalupe G. Fisher, Jennifer Orlet O'Connor, Teresia M. Hughes, Sheryl O. TI Emotion regulation strategies and childhood obesity in high risk preschoolers SO APPETITE LA English DT Article DE Hispanic preschoolers; Child eating self-regulation; Child weight status; Executive functioning; Emotional regulation; Delay of gratification ID SELF-REGULATION; WEIGHT-GAIN; CHILDREN; GRATIFICATION; DELAY; ABILITY; PREDICTORS; ABSENCE; HUNGER AB The current study examined the relationships between the specific strategies that preschool children use to regulate their emotions and childhood weight status to see if emotion regulation strategies would predict childhood weight status over and above measures of eating self-regulation. 185 4- to 5-year-old Latino children were recruited through Head Start centers in a large city in the southeastern U.S. Children completed both a delay of gratification task (emotion regulation) and an eating in the absence of hunger task (eating regulation). Eating regulation also was assessed by maternal reports. Four emotion regulation strategies were examined in the delay of gratification task: shut out stimuli, prevent movement, distraction, and attention to reward. Hierarchical linear regressions predicting children's weight status showed that both measures of eating regulation negatively predicted child obesity, and the use of prevent movement negatively predicted child obesity. Total wait time during the delay of gratification tasks was not a significant predictor. The current findings are consistent with studies showing that for preschool children, summary measures of emotion regulation (e.g., wait time) are not concurrently associated with child obesity. In contrast, the use of emotion regulation strategies was a significant predictor of lower child weight status. These findings help identify emotion regulation strategies that prevention programs can target for helping children regulate their emotions and decrease their obesity risk. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Power, Thomas G.; Olivera, Yadira A.; Hill, Rachael A.; Beck, Ashley D.; Hopwood, Veronica; Garcia, Karina Silva; Ramos, Guadalupe G.] Washington State Univ, Dept Human Dev, POB 6452, Pullman, WA 99164 USA. [Fisher, Jennifer Orlet] Temple Univ, Ctr Obes Res & Educ, 3223 N Broad St,Suite 175, Philadelphia, PA 19140 USA. [O'Connor, Teresia M.; Hughes, Sheryl O.] Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, 1100 Bates, Houston, TX 77030 USA. RP Hughes, SO (reprint author), Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, 1100 Bates, Houston, TX 77030 USA. EM tompower@wsu.edu; yadi.olivera@wsu.edu; rachael.hill@wsu.edu; aeaton@wsu.edu; v.bonilla-pcaheco@wsu.edu; kari.silva@wsu.edu; guadalupe.g.ramos@wsu.edu; jofisher@temple.edu; teresiao@bcm.edu; shughes@bcm.edu FU National Institute of Child Health and Human Development [R01HD062567]; USDA/ARS [6250-51000] FX This research was supported by funds from the National Institute of Child Health and Human Development (Grant R01HD062567). This work is also a publication of the U.S. Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine (Houston, TX) funded in part by the USDA/ARS (Cooperative Agreement 6250-51000). The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of tradenames, commercial products, or organizations imply endorsement from the US government. We would like to acknowledge the following people who were instrumental in collecting and coding the data for this study: Nilda Micheli, Monica Lopez, Kayla Weinmann, and Noemi Aguilar. NR 26 TC 0 Z9 0 U1 24 U2 24 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0195-6663 EI 1095-8304 J9 APPETITE JI Appetite PD DEC 1 PY 2016 VL 107 BP 623 EP 627 DI 10.1016/j.appet.2016.09.008 PG 5 WC Behavioral Sciences; Nutrition & Dietetics SC Behavioral Sciences; Nutrition & Dietetics GA EE2BP UT WOS:000389389000072 PM 27620645 ER PT J AU Townsend, MS Shifts, MK Styne, DM Drake, C Lanoue, L Woodhouse, L Allen, LH AF Townsend, Marilyn S. Shifts, Mical K. Styne, Dennis M. Drake, Christiana Lanoue, Louise Woodhouse, Leslie Allen, Lindsay H. TI Vegetable behavioral tool demonstrates validity with My Plate vegetable cups and carotenoid and inflammatory biomarkers SO APPETITE LA English DT Article DE Vegetables; Assessment tool; Young children; Preschool; Validation; Evaluation ID NUTRITION EDUCATION; FRUIT; OBESITY; CONSUMPTION; AVAILABILITY; CHECKLIST; PATTERNS; PROGRAMS; QUALITY; WOMEN AB Young children are not meeting recommendations for vegetable intake. Our objective is to provide evidence of validity and reliability for a pictorial vegetable behavioral assessment for use by federally funded community nutrition programs. Parent/child pairs (n=133) from Head Start and the Special Supplemental Nutrition Program for Women, Infants and Children [WIC] provided parent-administered vegetable tools, three child 24-hour diet recalls, child blood sample and measured heights/weights. The 10-item Focus on Veggies scale, with an alpha of .83 and a stability reliability coefficient of .74, was positively related to vegetables in cup equivalents [p <=.05]; dietary intakes of folate, vitamin C, beta-carotene, potassium and magnesium [p <=.05-.01]; and soluble fiber [p <=.001]. The child vegetable scores were related to the parent's mediators [p <=.00001] and vegetable behaviors [p <=.000011]. Children's plasma inflammatory markers were negatively related to the 10 item scale [p <=.05] and are indicators of the child's health status. The positive relationship between the serum carotenoid index and a sub-scale of child vegetable behaviors offered additional support for criterion validity [p<.05]. Finally, the inverse relationship of BMI-for-age percentile one year post baseline and a sub-scale of child vegetable behaviors supported the predictive validity [p <=.05]. Focus on Veggies, a simple assessment tool, can inform practitioners about the child's health status. A child with a high score, shows a healthful profile with a lower inflammation index, higher carotenoid index, lower BMI and higher vegetable intake. In conclusion, validity of Focus on Veggies has been demonstrated using vegetable cup equivalents and micronutrient intakes, anthropometry and blood biomarkers. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Townsend, Marilyn S.; Lanoue, Louise] Univ Calif Davis, Dept Nutr, One Shields Ave, Davis, CA 95616 USA. [Shifts, Mical K.] Calif State Univ Sacramento, Dept Family Studies, Sacramento, CA 95819 USA. [Styne, Dennis M.] Univ Calif, Davis Med Sch, Dept Pediat, Pediat Endocrinol, Sacramento, CA USA. [Drake, Christiana] Univ Calif, Dept Stat, Davis, CA USA. [Woodhouse, Leslie; Allen, Lindsay H.] USDA, Western Human Nutr Res Ctr, Davis, CA USA. RP Townsend, MS (reprint author), Univ Calif Davis, Dept Nutr, One Shields Ave, Davis, CA 95616 USA. EM mstownsend@ucdavis.edu FU National Institute of Food and Agriculture, U.S. Department of Agriculture [2009-55215-05019, 2010-85215-20658] FX This material is based upon work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award numbers #2009-55215-05019 & #2010-85215-20658. NR 40 TC 0 Z9 0 U1 10 U2 10 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0195-6663 EI 1095-8304 J9 APPETITE JI Appetite PD DEC 1 PY 2016 VL 107 BP 628 EP 638 DI 10.1016/j.appet.2016.09.002 PG 11 WC Behavioral Sciences; Nutrition & Dietetics SC Behavioral Sciences; Nutrition & Dietetics GA EE2BP UT WOS:000389389000073 PM 27603783 ER PT J AU Mete, A Gharpure, R Pitesky, ME Famini, D Sverlow, K Dunn, J AF Mete, Asli Gharpure, Radhika Pitesky, Maurice E. Famini, Dan Sverlow, Karen Dunn, John TI Marek's Disease in Backyard Chickens, A Study of Pathologic Findings and Viral Loads in Tumorous and Nontumorous Birds SO AVIAN DISEASES LA English DT Article DE backyard chickens; Marek's disease; MDV serotypes; real-time PCR; absolute quantitation; pathology; histopathology ID POLYMERASE-CHAIN-REACTION; TIME QUANTITATIVE PCR; ADULT CHICKENS; VIRUS; DIAGNOSIS; SAMPLES; VACCINATION; CHALLENGE; FEATHER; TISSUES AB Marek's disease (MD) is a major cause of mortality in backyard chickens. The diagnosis of MD is complex, however, and knowledge of Marek's disease virus (MDV) in spontaneous field cases such as in backyard chickens is largely unknown. In this study, 40 backyard chickens with a presumptive MD diagnosis based on histologic lymphoid infiltrations in peripheral nerves with and without lymphomas were investigated. Twenty-eight of the birds were submitted to the diagnostic laboratory for disease explorations, and 12 chickens were from a flock in which some members demonstrated anisocoria and pupil irregularities compatible with ocular MD. Histologic scores were established for brain, peripheral nerves, heart, lung, liver, kidney, and gonad sections, ranging from mild (+) to severe (+++) lymphoid infiltrations. Twelve chickens had gross lymphomas, and all but two chickens had mild to severe peripheral nerve lymphoid infiltrates. There were no age or breed predispositions in the study group. Quantification of serotypes MDV-1, -2, and -3 performed with real-time PCR demonstrated high correlation (R-2 = 0.94) between fresh and fixed spleen specimens, as well as between histopathology scores and MDV-1 viral loads. MDV-2 DNA was detected in a portion of the chickens, likely consistent with naturally occurring virus, whereas the vaccine strain MDV-3 was rarely detected. Significant differences in MDV-1 viral loads between tumorous and nontumorous chickens were observed, in which a ratio of MDV-1 glycoprotein B/glyceraldehyde-3-phosphate dehydrogenase >= 0.5 was suggestive of gross tumors in this study. We propose that real-time PCR may be a good tool for MD diagnosis in backyard chickens. C1 [Mete, Asli; Sverlow, Karen] Univ Calif Davis, Calif Anim Hlth & Safety Lab, Davis, CA 95616 USA. [Gharpure, Radhika] Univ Calif Davis, Sch Vet Med, Davis, CA 95616 USA. [Pitesky, Maurice E.] Univ Calif Davis, Dept Populat Hlth & Reprod, Sch Vet Med Cooperat Extens, Davis, CA 95616 USA. [Famini, Dan] Santa Rosa Jr Coll, Dept Agr, 1501 Mendocino Ave, Santa Rosa, CA 95401 USA. [Dunn, John] ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. RP Mete, A (reprint author), Univ Calif Davis, Calif Anim Hlth & Safety Lab, Davis, CA 95616 USA. EM amete@ucdavis.edu FU Center for Companion Animal Health, School of Veterinary Medicine, University of California, Davis FX This work was supported by the Center for Companion Animal Health, School of Veterinary Medicine, University of California, Davis. NR 44 TC 0 Z9 0 U1 2 U2 2 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD DEC PY 2016 VL 60 IS 4 BP 826 EP 836 PG 11 WC Veterinary Sciences SC Veterinary Sciences GA EE5QK UT WOS:000389662600016 PM 27902909 ER PT J AU Leathers, TD Nunnally, MS Stanley, AM Rich, JO AF Leathers, Timothy D. Nunnally, Melinda S. Stanley, April M. Rich, Joseph O. TI Utilization of corn fiber for production of schizophyllan SO BIOMASS & BIOENERGY LA English DT Article DE Corn fiber; Corn steep liquor; Corn syrup; Schizophyllan ID PRETREATMENT METHODS AB Corn fiber is an abundant lignocellulosic biomass resource produced during the wet milling of corn. Although corn fiber is recalcitrant to enzymatic digestion, the fungus Schizophyllum commune was able to directly utilize corn fiber for production of the valuable bioproduct schizophyllan. Schizophyllan is a biopolymer composed entirely of glucose, with a [beta-1,3-linked backbone and single beta-1,6-linked glucose side chains at every third residue. Schizophyllan is being developed for bulk biomaterial applications, such as in enhanced oil recovery and as a component of biolubricants. S. commune strain ATCC 38548 produced up to 6.8 +/- 0.2 g dm(-3) schizophyllan when grown in malt extract medium containing 10 g dm(-3) untreated corn fiber in place of glucose. Pretreatment of corn fiber with alkaline hydrogen peroxide enhanced yields of schizophyllan. Corn steep liquor at 50 cm(3) dm(-3) could replace malt extract as a nitrogen source, producing up to 5.4 +/- 1.6 g dm(-3) schizophyllan and 6.0 +/- 0.5 g dm(-3) schizophyllan from 10 g dm(-3) untreated and pretreated corn fiber, respectively. Glucose (from corn syrup) further enhanced yields, substituting for the maltose component of malt extract. Schizophyllan produced from corn fiber exhibited a high molecular weight of 3.2 x 10(7) Da, with solution viscosity properties characteristic of schizophyllan. Utilization of corn fiber could reduce the cost of schizophyllan production and provide a value-added coproduct from corn processing biomass. Published by Elsevier Ltd. C1 [Leathers, Timothy D.; Nunnally, Melinda S.; Stanley, April M.; Rich, Joseph O.] ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 North Univ St, Peoria, IL 61604 USA. RP Leathers, TD (reprint author), ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 North Univ St, Peoria, IL 61604 USA. EM tim.leathers@ars.usda.gov FU National Corn Growers Association [58-3620-4-003] FX This work was supported in part by Reimbursable Cooperative Agreement 58-3620-4-003 from the National Corn Growers Association. The authors thank Gordon W. Selling and Kelly Utt for performing FTIR analyses. NR 8 TC 0 Z9 0 U1 3 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 EI 1873-2909 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD DEC PY 2016 VL 95 BP 132 EP 136 DI 10.1016/j.biombioe.2016.10.001 PG 5 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA EE0SJ UT WOS:000389288900016 ER PT J AU Baker, SC Halpern, CB Wardlaw, TJ Kern, C Edgar, GJ Thomson, RJ Bigley, RE Franklin, JF Gandhi, KJK Gustafson, L Johnson, S Palik, BJ Spies, TA Steel, EA Weslien, J Strengbom, J AF Baker, Susan C. Halpern, Charles B. Wardlaw, Timothy J. Kern, Christel Edgar, Graham J. Thomson, Russell J. Bigley, Richard E. Franklin, Jerry F. Gandhi, Kamal J. K. Gustafson, Lena Johnson, Samuel Palik, Brian J. Spies, Thomas A. Steel, E. Ashley Weslien, Jan Strengbom, Joachim TI A cross-continental comparison of plant and beetle responses to retention of forest patches during timber harvest SO ECOLOGICAL APPLICATIONS LA English DT Article DE aggregated retention; forest influence; ground-active beetles; lifeboating; retention forestry ID GREEN-TREE RETENTION; NATURAL DISTURBANCE; PACIFIC-NORTHWEST; DWELLING BEETLES; BIODIVERSITY; EDGE; CONSERVATION; ASSEMBLAGES; MANAGEMENT; PATTERNS AB Timber harvest can adversely affect forest biota. Recent research and application suggest that retention of mature forest elements (retention forestry), including unharvested patches (or aggregates) within larger harvested units, can benefit biodiversity compared to clearcutting. However, it is unclear whether these benefits can be generalized among the diverse taxa and biomes in which retention forestry is practiced. Lack of comparability in methods for sampling and analyzing responses to timber harvest and edge creation presents a challenge to synthesis. We used a consistent methodology (similarly spaced plots or traps along transects) to investigate responses of vascular plants and ground-active beetles to aggregated retention at replicate sites in each of four temperate and boreal forest types on three continents: Douglas-fir forests in Washington, USA; aspen forests in Minnesota, USA; spruce forests in Sweden; and wet eucalypt forests in Tasmania, Australia. We assessed (1) differences in local (plot-scale) species richness and composition between mature (intact) and regenerating (previously harvested) forest; (2) the lifeboating function of aggregates (capacity to retain species of unharvested forest); and whether intact forests and aggregates (3) are susceptible to edge effects and (4) influence the adjacent regenerating forest. Intact and harvested forests differed in composition but not richness of plants and beetles. The magnitude of this difference was generally similar among regions, but there was considerable heterogeneity of composition within and among replicate sites. Aggregates within harvest units were effective at lifeboating for both plant and beetle communities. Edge effects were uncommon even within the aggregates. In contrast, effects of forest influence on adjacent harvested areas were common and as strong for aggregates as for larger blocks of intact forest. Our results provide strong support for the widespread application of aggregated retention in boreal and temperate forests. The consistency of pattern in four very different regions of the world suggests that, for forest plants and beetles, responses to aggregated retention are likely to apply more widely. Our results suggest that through strategic placement of aggregates, it is possible to maintain the natural heterogeneity and biodiversity of mature forests managed for multiple objectives. C1 [Baker, Susan C.; Wardlaw, Timothy J.] Univ Tasmania, Sch Biol Sci, Private Bag 55, Hobart, Tas 7001, Australia. [Baker, Susan C.; Wardlaw, Timothy J.] Univ Tasmania, ARC Ctr Forest Value, Private Bag 55, Hobart, Tas 7001, Australia. [Baker, Susan C.; Halpern, Charles B.; Franklin, Jerry F.] Univ Washington, Sch Environm & Forest Sci, Box 352100, Seattle, WA 98195 USA. [Kern, Christel; Strengbom, Joachim] USDA Forest Serv, Northern Res Stn, 5985 Highway K, Rhinelander, WI 54501 USA. [Edgar, Graham J.; Thomson, Russell J.] Univ Tasmania, Inst Marine & Antarctic Studies, GPO Box 252-49, Hobart, Tas 7001, Australia. [Bigley, Richard E.] Washington State Dept Nat Resources, Forest Resources, Olympia, WA 98504 USA. [Gandhi, Kamal J. K.] Univ Georgia, Daniel B Warnell Sch Forestry & Nat Resources, 180 E Green St, Athens, GA 30602 USA. [Gustafson, Lena; Johnson, Samuel] Swedish Univ Agr Sci SLU, Dept Ecol, POB 7044, SE-75007 Uppsala, Sweden. [Palik, Brian J.] USDA Forest Serv, Northern Res Stn, 1831 Hwy 169 E, Grand Rapids, MN 55744 USA. [Spies, Thomas A.] USDA Forest Serv, PNW Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. [Steel, E. Ashley] USDA Forest Serv, PNW Res Stn, 400 N 34th St,Suite 201, Seattle, WA 98103 USA. [Weslien, Jan] Forestry Res Inst Sweden Skogforsk, SE-75143 Uppsala, Sweden. RP Baker, SC (reprint author), Univ Tasmania, Sch Biol Sci, Private Bag 55, Hobart, Tas 7001, Australia.; Baker, SC (reprint author), Univ Tasmania, ARC Ctr Forest Value, Private Bag 55, Hobart, Tas 7001, Australia.; Baker, SC (reprint author), Univ Washington, Sch Environm & Forest Sci, Box 352100, Seattle, WA 98195 USA. EM sue.baker@forestrytas.com.au RI Thomson, Russell/H-5653-2012 OI Thomson, Russell/0000-0003-4949-4120 FU Australian Research Council [LP100100050, LP140100075]; Forestry Tasmania; Fulbright Scholarship FX This research was funded by the Australian Research Council Linkage grants LP100100050 and LP140100075, Forestry Tasmania, and a Fulbright Scholarship awarded to S. C. Baker. We are grateful to participants at a retention-forestry workshop in Sweden in 2011 for discussion that led to the final study-design. We thank the many people who provided logistical support in all four regions, including access to study sites, assistance with field work, species identifications, and data-entry. Finally, we thank the anonymous reviewers for their constructive comments on the manuscript. NR 54 TC 1 Z9 1 U1 9 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD DEC PY 2016 VL 26 IS 8 BP 2493 EP 2504 PG 12 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA EE1BU UT WOS:000389316400012 PM 27787926 ER PT J AU Buotte, PC Hicke, JA Preisler, HK Abatzoglou, JT Raffa, KF Logan, JA AF Buotte, Polly C. Hicke, Jeffrey A. Preisler, Haiganoush K. Abatzoglou, John T. Raffa, Kenneth F. Logan, Jesse A. TI Climate influences on whitebark pine mortality from mountain pine beetle in the Greater Yellowstone Ecosystem SO ECOLOGICAL APPLICATIONS LA English DT Article DE climate change; Dendroctonus ponderosae Hopkins; forest disturbance; Greater Yellowstone Ecosystem; logistic regression; Pinus albicaulis; Pinus contorta var. latifolia ID WESTERN UNITED-STATES; NORTH-AMERICAN FORESTS; CHANGE-TYPE DROUGHT; BARK BEETLES; DENDROCTONUS-PONDEROSAE; TREE MORTALITY; SPATIOTEMPORAL PATTERNS; AGE STRUCTURE; DIE-OFF; COLEOPTERA AB Extensive mortality of whitebark pine, beginning in the early to mid-2000s, occurred in the Greater Yellowstone Ecosystem (GYE) of the western USA, primarily from mountain pine beetle but also from other threats such as white pine blister rust. The climatic drivers of this recent mortality and the potential for future whitebark pine mortality from mountain pine beetle are not well understood, yet are important considerations in whether to list whitebark pine as a threatened or endangered species. We sought to increase the understanding of climate influences on mountain pine beetle outbreaks in whitebark pine forests, which are less well understood than in lodgepole pine, by quantifying climate-beetle relationships, analyzing climate influences during the recent outbreak, and estimating the suitability of future climate for beetle outbreaks. We developed a statistical model of the probability of whitebark pine mortality in the GYE that included temperature effects on beetle development and survival, precipitation effects on host tree condition, beetle population size, and stand characteristics. Estimated probability of whitebark pine mortality increased with higher winter minimum temperature, indicating greater beetle winter survival; higher fall temperature, indicating synchronous beetle emergence; lower two-year summer precipitation, indicating increased potential for host tree stress; increasing beetle populations; stand age; and increasing percent composition of whitebark pine within a stand. The recent outbreak occurred during a period of higher-than-normal regional winter temperatures, suitable fall temperatures, and low summer precipitation. In contrast to lodgepole pine systems, area with mortality was linked to precipitation variability even at high beetle populations. Projections from climate models indicate future climate conditions will likely provide favorable conditions for beetle outbreaks within nearly all current whitebark pine habitat in the GYE by the middle of this century. Therefore, when surviving and regenerating trees reach ages suitable for beetle attack, there is strong potential for continued whitebark pine mortality due to mountain pine beetle. C1 [Buotte, Polly C.] Univ Idaho, Environm Sci Program, Moscow, ID 83844 USA. [Buotte, Polly C.; Hicke, Jeffrey A.; Abatzoglou, John T.] Univ Idaho, Dept Geog, Moscow, ID 83844 USA. [Preisler, Haiganoush K.] USDA Forest Serv, Pacific Southwest Res Stn, Albany, CA 94710 USA. [Raffa, Kenneth F.] Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA. [Logan, Jesse A.] USDA Forest Serv, Emigrant, MT 59027 USA. RP Buotte, PC (reprint author), Univ Idaho, Environm Sci Program, Moscow, ID 83844 USA.; Buotte, PC (reprint author), Univ Idaho, Dept Geog, Moscow, ID 83844 USA. EM pbuotte@uidaho.edu FU Department of the Interior Northwest Climate Science Center (NW CSC) through United States Geological Survey (USGS) [G12AC20481]; US Geological Survey's Ecosystems and Climate and Land Use Change mission areas through the Western Mountain Initiative project; Agriculture and Food Research Initiative of the National Institute of Food and Agriculture [2013-67003-20652] FX This project was supported by the Department of the Interior Northwest Climate Science Center (NW CSC) through a Cooperative Agreement (G12AC20481) from the United States Geological Survey (USGS). Its contents are solely the responsibility of the authors and do not necessarily represent the views of the NW CSC or the USGS. This manuscript is submitted for publication with the understanding that the United States Government is authorized to reproduce and distribute reprints for governmental purposes. Support was also provided by the US Geological Survey's Ecosystems and Climate and Land Use Change mission areas through the Western Mountain Initiative project and the Agriculture and Food Research Initiative of the National Institute of Food and Agriculture through grant #2013-67003-20652. We acknowledge the World Climate Research Programme's Working Group on Coupled Modelling, which is responsible for CMIP, and thank the climate modeling groups (listed in Appendix S1: Table S1 of this paper) for producing and making available their model output. We thank several reviewers whose thoughtful comments improved the manuscript. We have no conflicts of interest to declare. NR 99 TC 0 Z9 0 U1 30 U2 30 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD DEC PY 2016 VL 26 IS 8 BP 2505 EP 2522 DI 10.1002/eap.1396 PG 18 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA EE1BU UT WOS:000389316400013 PM 27907251 ER PT J AU Bateman, BL Pidgeon, AM Radeloff, VC Flather, CH VanDerWal, J Akakaya, HR Thogmartin, WE Albright, TP Vavrus, SJ Heglund, PJ AF Bateman, Brooke L. Pidgeon, Anna M. Radeloff, Volker C. Flather, Curtis H. VanDerWal, Jeremy Akakaya, H. Resit Thogmartin, Wayne E. Albright, Thomas P. Vavrus, Stephen J. Heglund, Patricia J. TI Potential breeding distributions of US birds predicted with both short-term variability and long-term average climate data SO ECOLOGICAL APPLICATIONS LA English DT Article DE climate change; guilds; Maxent; North American breeding birds; species distribution model; species range ID SPECIES DISTRIBUTION MODELS; POPULATION-DYNAMICS; SOUTHERN ILLINOIS; ENVELOPE MODELS; AVIAN MORTALITY; HEAT WAVES; WEATHER; EVENTS; PATTERNS; NORTH AB Climate conditions, such as temperature or precipitation, averaged over several decades strongly affect species distributions, as evidenced by experimental results and a plethora of models demonstrating statistical relations between species occurrences and long-term climate averages. However, long-term averages can conceal climate changes that have occurred in recent decades and may not capture actual species occurrence well because the distributions of species, especially at the edges of their range, are typically dynamic and may respond strongly to short-term climate variability. Our goal here was to test whether bird occurrence models can be predicted by either covariates based on short-term climate variability or on long-term climate averages. We parameterized species distribution models (SDMs) based on either short-term variability or long-term average climate covariates for 320 bird species in the conterminous USA and tested whether any life-history trait-based guilds were particularly sensitive to short-term conditions. Models including short-term climate variability performed well based on their cross-validated area-under-the-curve AUC score (0.85), as did models based on long-term climate averages (0.84). Similarly, both models performed well compared to independent presence/absence data from the North American Breeding Bird Survey (independent AUC of 0.89 and 0.90, respectively). However, models based on short-term variability covariates more accurately classified true absences for most species (73% of true absences classified within the lowest quarter of environmental suitability vs. 68%). In addition, they have the advantage that they can reveal the dynamic relationship between species and their environment because they capture the spatial fluctuations of species potential breeding distributions. With this information, we can identify which species and guilds are sensitive to climate variability, identify sites of high conservation value where climate variability is low, and assess how species' potential distributions may have already shifted due recent climate change. However, long-term climate averages require less data and processing time and may be more readily available for some areas of interest. Where data on short-term climate variability are not available, long-term climate information is a sufficient predictor of species distributions in many cases. However, short-term climate variability data may provide information not captured with long-term climate data for use in SDMs. C1 [Bateman, Brooke L.; Pidgeon, Anna M.; Radeloff, Volker C.] Univ Wisconsin, Dept Forest & Wildlife Ecol, SILVIS Lab, Madison, WI 53706 USA. [Flather, Curtis H.] USDA Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. [VanDerWal, Jeremy] James Cook Univ, Sch Marine & Trop Biol, Ctr Trop Biodivers & Climate Change Res, Townsville, Qld 4811, Australia. [VanDerWal, Jeremy] James Cook Univ, Div Res & Innovat, Townsville, Qld 4811, Australia. [Akakaya, H. Resit] SUNY Stony Brook, Dept Ecol & Evolut, Stony Brook, NY 11794 USA. [Thogmartin, Wayne E.] US Geol Survey, Upper Midwest Environm Sci Ctr, La Crosse, WI 54603 USA. [Albright, Thomas P.] Univ Nevada, Lab Conservat Biogeog, Dept Geog, Reno, NV 89577 USA. [Albright, Thomas P.] Univ Nevada, Lab Conservat Biogeog, Program Ecol Evolut & Conservat Biol, Reno, NV 89577 USA. [Vavrus, Stephen J.] Univ Wisconsin, Ctr Climate Res, Madison, WI 53706 USA. [Heglund, Patricia J.] US Fish & Wildlife Serv, La Crosse, WI 54603 USA. RP Bateman, BL (reprint author), Univ Wisconsin, Dept Forest & Wildlife Ecol, SILVIS Lab, Madison, WI 53706 USA. EM brooke.bateman@gmail.com RI Thogmartin, Wayne/A-4461-2008 OI Thogmartin, Wayne/0000-0002-2384-4279 FU NASA [NNH10ZDA001N-BIOCLIM]; James Cook University High Performance Computing (HPC) unit FX We gratefully acknowledge support for this research by the NASA Biodiversity Program and the Climate and Biological Response funding opportunity (NNH10ZDA001N-BIOCLIM) and the James Cook University High Performance Computing (HPC) unit. In addition, we thank T. Robertson and the Global Biodiversity and Information Facility (GBIF) for assistance in obtaining bird occurrence data for the USA and the numerous contributors of occurrence data to GBIF (please see full list of bird data sources in Appendix S1). Associate editor J. Elith and two anonymous reviewers provided very helpful and constructive comments that greatly improved our manuscript. We thank E. Wood for his help in developing guild descriptions, as well as P. Culbert, J. Gorzo, and A. Allstadt for general assistance and discussions. Any use of trade, product, or firm names are for descriptive purposes only and do not imply endorsement by the U.S. Government. The views expressed in this article are the authors' own and do not necessarily represent the views of the U.S. Fish and Wildlife Service. NR 72 TC 0 Z9 0 U1 14 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD DEC PY 2016 VL 26 IS 8 BP 2718 EP 2729 PG 12 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA EE1BU UT WOS:000389316400028 PM 27907262 ER PT J AU Rossman, S Yackulic, CB Saunders, SP Reid, J Davis, R Zipkin, EF AF Rossman, Sam Yackulic, Charles B. Saunders, Sarah P. Reid, Janice Davis, Ray Zipkin, Elise F. TI Dynamic N-occupancy models: estimating demographic rates and local abundance from detection-nondetection data SO ECOLOGY LA English DT Article DE barred owl; demographic rates; dynamic; heterogeneity; latent; N-mixture model; occupancy; species distribution models ID ESTIMATING SITE OCCUPANCY; NORTHERN SPOTTED OWLS; PRESENCE-ABSENCE DATA; BARRED OWLS; DETECTION PROBABILITIES; POPULATION-DYNAMICS; HABITAT; COUNTS; DISTRIBUTIONS; EXPANSION AB Occupancy modeling is a widely used analytical technique for assessing species distributions and range dynamics. However, occupancy analyses frequently ignore variation in abundance of occupied sites, even though site abundances affect many of the parameters being estimated (e.g., extinction, colonization, detection probability). We introduce a new model (dynamic N-occupancy) capable of providing accurate estimates of local abundance, population gains (reproduction/immigration), and apparent survival probabilities while accounting for imperfect detection using only detection/nondetection data. Our model utilizes heterogeneity in detection based on variations in site abundances to estimate latent demographic rates via a dynamic N-mixture modeling framework. We validate our model using simulations across a wide range of values and examine the data requirements, including the number of years and survey sites needed, for unbiased and precise estimation of parameters. We apply our model to estimate spatiotemporal heterogeneity in abundances of barred owls (Strix varia) within a recently invaded region in Oregon (USA). Estimates of apparent survival and population gains are consistent with those from a nearby radio-tracking study and elucidate how barred owl abundances have increased dramatically over time. The dynamic N-occupancy model greatly improves inferences on individual-level population processes from occupancy data by explicitly modeling the latent population structure. C1 [Rossman, Sam; Saunders, Sarah P.; Zipkin, Elise F.] Michigan State Univ, Coll Nat Sci, Dept Integrat Biol, 288 Farm Lane RM 203, E Lansing, MI 48824 USA. [Rossman, Sam] Hubbs Sea World Res Inst, 3830 S Highway A1A 4-181, Melbourne Beach, FL 32951 USA. [Yackulic, Charles B.] US Geol Survey, Southwest Biol Sci Ctr, 2255 N Gemini Dr, Flagstaff, AZ 86001 USA. [Reid, Janice; Davis, Ray] US Forest Serv, Pacific Northwest Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. [Zipkin, Elise F.] Michigan State Univ, Ecol Evolutionary Biol & Behav Program, 293 Farm Lane RM 103, E Lansing, MI 48824 USA. RP Rossman, S (reprint author), Michigan State Univ, Coll Nat Sci, Dept Integrat Biol, 288 Farm Lane RM 203, E Lansing, MI 48824 USA.; Rossman, S (reprint author), Hubbs Sea World Res Inst, 3830 S Highway A1A 4-181, Melbourne Beach, FL 32951 USA. EM rossmans@msu.edu FU John Wesley Powell Center for Analysis and Synthesis; USGS; NPS/USGS National Park Monitoring Program FX We are grateful to Michigan State University's High Performance Computing Center for computational support and to the NPS/USGS National Park Monitoring Program for funding that supported model development. The work in this manuscript grew out of discussions from a working group supported by the John Wesley Powell Center for Analysis and Synthesis, funded by the USGS. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. Barred owl photo by Mdf (CC BY-SA 3.0) obtained from Wikimedia Commons. NR 29 TC 1 Z9 1 U1 18 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0012-9658 EI 1939-9170 J9 ECOLOGY JI Ecology PD DEC PY 2016 VL 97 IS 12 BP 3300 EP 3307 DI 10.1002/ecy.1598 PG 8 WC Ecology SC Environmental Sciences & Ecology GA EE2WC UT WOS:000389444800007 PM 27912019 ER PT J AU Ngo, H Latona, R Wagner, KM Nunez, A Ashby, R Dunn, RO AF Ngo, Helen Latona, Renee Wagner, Karen M. Nunez, Alberto Ashby, Richard Dunn, Robert O. TI Synthesis and low temperature characterization of iso-oleic ester derivatives SO EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY LA English DT Article DE Cloud point; Differential scanning calorimetry; Iso-oleic acid; Pressurized-differential scanning calorimetry; Thermogravimetric analysis ID ACID METHYL-ESTERS; CRYSTAL-STRUCTURE; STRUCTURAL-CHARACTERIZATION; PHASE-BEHAVIOR; FATTY; BIODIESEL; STEARATE; INTERESTERIFICATION; STABILITY; ISOMERS AB Three new iso-oleic ester derivatives (i.e., isopropyl ester (IOA-iPrE, 6), n-butyl ester (IOA-n-BuE, 7), and 2-ethylhexyl ester (IOA-2-EHE, 8)) were synthesized from iso-oleic acid (IOA, 4) using a standard esterification method. These esterified alcohols were chosen because of their bulky and branched-chain alkyl groups which can reduce melting point in comparison to a smaller alkyl group such as methyl ester. The differential scanning calorimetry (DSC), cloud point and pour point results showed that esters 6, 7, and 8 had much lower melting transition temperatures and cold flow properties than the methyl (IOA-FAME, 5) and parent 4. The oxidative and thermal stability by pressure (P-DSC) and thermogravimetric analysis (TGA) results also showed a very similar trend where the bulkier and branched-chain alkyl esters had better stability than the smaller headgroup esters. Practical applications: Since saturated fats have high melting points, they are solids which can be harmful to our health and can cause damage to machinery. There is an urgent need to develop methods to produce fats with low melting points to replace the unwanted fats. In this paper, a series of modified branched-chain fatty acid ester derivatives (i.e., iso-oleic acid esters) that are liquid at room temperature with enhanced fluidity were synthesized. These ester fats have been found to perform much better than the original fatty acids and saturated fats. These findings are important as these iso-oleic ester fats can potentially replace solid materials which are often problematic at low temperatures. Iso-oleic ester derivatives containing bulky and branched-chain alkyl groups exhibit reduce melting point in comparison to a smaller alkyl group such as methyl ester. C1 [Ngo, Helen; Latona, Renee; Wagner, Karen M.; Nunez, Alberto; Ashby, Richard] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA USA. [Dunn, Robert O.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL USA. RP Ngo, H (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM helen.ngo@ars.usda.gov FU NIFA-AFRI [2013-67021-21142] FX The authors would like to thank Ms. Megan Hums and Dr. Richard Cairncross from Drexel University for the help on the AV experiments. The authors thank Ms. Kim L. Ascherl for performing the ICP, cold flow property and P-DSC oxidation analyses. The authors would also like to thank NIFA-AFRI (grant award # 2013-67021-21142) for providing partial funding support. NR 32 TC 0 Z9 0 U1 4 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1438-7697 EI 1438-9312 J9 EUR J LIPID SCI TECH JI Eur. J. Lipid Sci. Technol. PD DEC PY 2016 VL 118 IS 12 BP 1915 EP 1925 DI 10.1002/ejlt.201500468 PG 11 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA EE9BZ UT WOS:000389922000010 ER PT J AU Niedz, RP Evens, TJ AF Niedz, Randall P. Evens, Terence J. TI Design of experiments (DOE)-history, concepts, and relevance to in vitro culture SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT LA English DT Review DE Experimental design; Scientific method; Plant tissue culture; Mineral nutrition; Ion-specific effects ID HARMFUL ALGAL BLOOMS; GROWTH; REPRODUCIBILITY; ELFVING,GUSTAV AB Design of experiments (DOE) is a large and well-developed field for understanding and improving the performance of complex systems. Because in vitro culture systems are complex and easily manipulated in controlled conditions, they are particularly well-suited for the application of DOE principles and techniques. Successful use of in vitro technologies in horticultural, plant breeding, or genetic applications typically involves improving some aspect of a system's growth response-organogenesis, somatic embryogenesis, metabolite biosynthesis, or responses required for crop improvement such as ploidy manipulation, embryo rescue, creation and manipulation of chimeras, somaclonal variation, and mutant isolation. How and why DOE is the appropriate research approach for developing and understanding in vitro systems research is explained. The presentation is a narrative of the historical context and the geometric basis of DOE to explain the underlying concepts. Examples illustrate the use of DOE in in vitro plant culture research. C1 [Niedz, Randall P.; Evens, Terence J.] ARS, USDA, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. RP Niedz, RP (reprint author), ARS, USDA, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM randall.niedz@ars.usda.gov NR 62 TC 0 Z9 0 U1 16 U2 16 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1054-5476 EI 1475-2689 J9 IN VITRO CELL DEV-PL JI In Vitro Cell. Dev. Biol.-Plant PD DEC PY 2016 VL 52 IS 6 BP 547 EP 562 DI 10.1007/s11627-016-9786-1 PG 16 WC Plant Sciences; Cell Biology; Developmental Biology SC Plant Sciences; Cell Biology; Developmental Biology GA EF0TA UT WOS:000390037700001 ER PT J AU Hand, CR Wada, N Stockwell, V Reed, BM AF Hand, Charles R. Wada, Nami Stockwell, Virginia Reed, Barbara M. TI Node position influences viability and contamination in hazelnut shoot explants SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT LA English DT Article DE Bacteria; Corylus avellana; Hazelnut; Micropropagation; Surface disinfestation ID MICROPROPAGATED MINT PLANTS; IN-VITRO PROPAGATION; ENDOPHYTIC BACTERIA; IDENTIFICATION; TISSUE; CULTURES; GROWTH AB Initiation of shoot cultures is difficult in many woody plants due to internal microbial contaminants and general lack of juvenility in material from the source plants. Hazelnuts (Corylus avellana L.) are generally difficult to initiate into culture for these same reasons. This study was designed to determine the effects of collection and surface disinfestation techniques and nodal position on the viability and contamination of shoot explants. In addition, culturable bacteria were identified on samples from surface-disinfested explants. Explants were collected from scion wood grafted onto seedling rootstocks and grown in a greenhouse. Single-node explants, excluding the shoot tip, were collected and the node location documented. After surface disinfestation, explants were held in liquid contaminant detection medium for 1 wk and the effect of this treatment on explant viability was evaluated. Node position was important for obtaining viable contaminant-free explants. Bacterial and fungal contaminations both increased with the distance from the shoot tip. The use of contaminant detection medium as a part of the initiation procedure did not affect viability. Explant-derived bacteria were identified as belonging to Brevundimonas sp. and Pseudomonas sp. through 16S rRNA sequence and APIA (R) tests. The best procedure for collecting axenic, viable hazelnut explants was to collect from the first three apical nodes, excluding the shoot tip, of actively growing greenhouse plants, place them in individual tubes for washing and surface disinfestation, and use contaminant detection techniques to identify contaminant-free cultures at initiation. C1 [Hand, Charles R.; Reed, Barbara M.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Wada, Nami] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Stockwell, Virginia] USDA ARS Hort Crops Res, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. [Reed, Barbara M.] USDA ARS Natl Clonal Germplasm Repository, 33447 Peoria Rd, Corvallis, OR 97333 USA. RP Reed, BM (reprint author), Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA.; Reed, BM (reprint author), USDA ARS Natl Clonal Germplasm Repository, 33447 Peoria Rd, Corvallis, OR 97333 USA. EM Reedba@onid.oregonstate.edu FU USDA-ARS CRIS [5358-21000-044-00D]; Oregon Hazelnut Commission FX This work was completed at the National Clonal Repository in Corvallis, OR and supported by USDA-ARS CRIS 5358-21000-044-00D and a grant from the Oregon Hazelnut Commission. NR 38 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1054-5476 EI 1475-2689 J9 IN VITRO CELL DEV-PL JI In Vitro Cell. Dev. Biol.-Plant PD DEC PY 2016 VL 52 IS 6 BP 580 EP 589 DI 10.1007/s11627-016-9791-4 PG 10 WC Plant Sciences; Cell Biology; Developmental Biology SC Plant Sciences; Cell Biology; Developmental Biology GA EF0TA UT WOS:000390037700004 ER PT J AU Ramesh, T Nayak, B Amirbahman, A Tripp, CP Mukhopadhyay, S AF Ramesh, Tamanna Nayak, Balunkeswar Amirbahman, Aria Tripp, Carl P. Mukhopadhyay, Sudarsan TI Application of ultraviolet light assisted titanium dioxide photocatalysis for food safety: A review SO INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES LA English DT Review DE Non-thermal technology; Titanium dioxide; Photocatalysis; Ultraviolet; Food safety ID HIGH HYDROSTATIC-PRESSURE; AQUEOUS CHLORINE DIOXIDE; ESCHERICHIA-COLI O157H7; FRESH APPLE JUICE; UV-C; LISTERIA-MONOCYTOGENES; PULSED-LIGHT; IN-VITRO; SEMICONDUCTOR PHOTOCATALYSIS; TIO2-UVC PHOTOCATALYSIS AB UV-assisted TiO2 photocatalysis (TUV) is an emerging non-thermal technology that has shown to efficiently inactivate food-borne pathogens. While TUV processing has been a well-established technique for water and wastewater treatment, its application in the food industry is minimal. The basic working principle underlying TUV technology is the photocatalytic production of reactive oxygen species (ROS) that carry out redox reactions with species adsorbed on the TiO2 surface. Studies have confirmed that severe cell damage including destruction of the cytoplasmic membrane, super-coiled plasmid DNA and genomic DNA, and internal organelles occurred when bacteria were exposed to TUV. Recently, TUV has been explored for its applications in surface disinfection and post-harvest disease control of fresh produce, pasteurization of beverages, and anti-microbial food packaging. Although the novel developments in the technology are promising, more research is needed to achieve the practical application of this technology. The intent of this article is to provide an overview of the principles of TUV, the mechanism of microbial inactivation by TUV, and the recent findings on utilization of TUV for microbiological safety of foods and also, to present the prospective opportunities for TUV in the food industry. Industrial relevance: TUV is a non-thermal technique recently explored for food safety applications. This review presents the prospective opportunities of TUV treatment in food industries in the area of food preservation. Based on the existing research findings discussed in this article, the authors have recommended major research aspects that are necessary to understand the scope of TUV technology in the food industry and validate the technology. This background will provide the necessary information for food scientists as well as industries to work on optimizing the treatment with regard to food quality and safety. Latest findings of TUV technology are promising; yet, more work is needed to develop and integrate extensive information for the practical application of this technology in the food industry. The areas that need more attention are listed and critically reviewed to facilitate utilization of the TUV technology in the food industry. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Ramesh, Tamanna; Nayak, Balunkeswar] Univ Maine, Food Sci & Human Nutr, Sch Food & Agr, Orono, ME 04469 USA. [Amirbahman, Aria] Univ Maine, Dept Civil & Environm Engn, Orono, ME 04469 USA. [Tripp, Carl P.] Univ Maine, Dept Chem, Orono, ME 04469 USA. [Tripp, Carl P.] Univ Maine, Lab Surface Sci & Technol, Orono, ME 04469 USA. [Mukhopadhyay, Sudarsan] ARS, Microbial Food Safety Residue Chem & Predict Micr, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Nayak, B (reprint author), Univ Maine, Food Sci & Human Nutr, Sch Food & Agr, Orono, ME 04469 USA. EM balunkeswar.nayak@maine.edu OI Nayak, Balunkeswar/0000-0001-5831-5193 FU United States Department of Agriculture, National Institute of Food and Agriculture (USDA NIFA) Hatch, Maine Agricultural and Forest Experiment Station, University of Maine, Orono, ME, USA [ME021408] FX This work was financially supported by the United States Department of Agriculture, National Institute of Food and Agriculture (USDA NIFA) Hatch No: ME021408, Maine Agricultural and Forest Experiment Station, University of Maine, Orono, ME, USA. The authors wish to thank Adeseye Lasekan and Dhriti Nayyar, graduate students in Food Science and Human Nutrition, School of Food and Agriculture, University of Maine, who assisted in the proof-reading of the manuscript. NR 108 TC 0 Z9 0 U1 25 U2 25 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1466-8564 EI 1878-5522 J9 INNOV FOOD SCI EMERG JI Innov. Food Sci. Emerg. Technol. PD DEC PY 2016 VL 38 BP 105 EP 115 DI 10.1016/j.ifset.2016.09.015 PN A PG 11 WC Food Science & Technology SC Food Science & Technology GA EE2ES UT WOS:000389397100013 ER PT J AU Phan, MN Guy, ES Nickson, RN Kao, CC AF Phan, Mimi N. Guy, Elizabeth S. Nickson, Ruby N. Kao, Christina C. TI Predictors and patterns of weight gain during treatment for tuberculosis in the United States of America SO INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES LA English DT Article DE Malnutrition; Underweight; Relapse ID MULTIDRUG-RESISTANT TUBERCULOSIS; BODY-COMPOSITION CHANGES; PULMONARY TUBERCULOSIS; NUTRITIONAL-STATUS; MALNUTRITION; RISK; TANZANIA; ADULTS AB Objectives: Patients with tuberculosis (TB) often present with weight loss. Lack of weight gain with TB treatment has been associated with treatment failure. The purpose of this study was to examine patterns of weight gain in patients with TB and determine the disease characteristics that predict weight gain. Methods: This was a retrospective cohort study of adults with TB treated in a county health system in the USA. Demographic, clinical, radiographic, and microbiological data were recorded in addition to monthly weights during treatment. Results: Overall, patients had a significant change in weight over the course of treatment (p < 0.0001). After 2 months of treatment, 31.9% of patients had gained at least 5% body weight; by the end of treatment, 62.4% of patients had gained at least 5% weight. Patients who gained weight did so in a linear fashion throughout treatment. Cavitary and extensive disease, a positive smear, and a positive culture were predictors of weight gain (p < 0.05). No patients had relapses during the time period of the study. Conclusions: Only a subset of patients treated for TB gain significant weight. A greater burden of disease was predictive of weight gain. (C) 2016 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. C1 [Phan, Mimi N.; Guy, Elizabeth S.; Nickson, Ruby N.; Kao, Christina C.] Baylor Coll Med, Dept Med, Sect Pulm Crit Care & Sleep, Houston, TX 77030 USA. [Kao, Christina C.] Baylor Coll Med, ARS, USDA, Childrens Nutr Res Ctr, 1100 Bates St,Room 7022, Houston, TX 77030 USA. RP Kao, CC (reprint author), Baylor Coll Med, Dept Med, Sect Pulm Crit Care & Sleep, Houston, TX 77030 USA.; Kao, CC (reprint author), Baylor Coll Med, ARS, USDA, Childrens Nutr Res Ctr, 1100 Bates St,Room 7022, Houston, TX 77030 USA. EM ck692121@bcm.edu NR 24 TC 0 Z9 0 U1 2 U2 2 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1201-9712 EI 1878-3511 J9 INT J INFECT DIS JI Int. J. Infect. Dis. PD DEC PY 2016 VL 53 BP 1 EP 5 DI 10.1016/j.ijid.2016.09.006 PG 5 WC Infectious Diseases SC Infectious Diseases GA EE3SA UT WOS:000389516700001 PM 27693292 ER PT J AU Love-Gregory, L Kraja, AT Allum, F Aslibekyan, S Hedman, AK Duan, Y Borecki, IB Arnett, DK McCarthy, MI Deloukas, P Ordovas, JM Hopkins, PN Grundberg, E Abumrad, NA AF Love-Gregory, Latisha Kraja, Aldi T. Allum, Fiona Aslibekyan, Stella Hedman, Asa K. Duan, Yanan Borecki, Ingrid B. Arnett, Donna K. McCarthy, Mark I. Deloukas, Panos Ordovas, Jose M. Hopkins, Paul N. Grundberg, Elin Abumrad, Nada A. TI Higher chylomicron remnants and LDL particle numbers associate with CD36 SNPs and DNA methylation sites that reduce CD36 SO JOURNAL OF LIPID RESEARCH LA English DT Article DE dietary lipids; lipoproteins; dyslipidemia; genetics; cholesterol; metabolism; cluster of differentiation 36; low density lipoprotein; single nucleotide polymorphism; deoxyribonucleic acid ID DENSITY-LIPOPROTEIN-CHOLESTEROL; DISEASE-ASSOCIATED VARIANTS; LIPID-LOWERING DRUGS; DIETARY FATTY-ACIDS; ADIPOSE-TISSUE; POSTPRANDIAL LIPEMIA; INSULIN-RESISTANCE; METABOLIC SYNDROME; GENETIC-VARIANTS; OBESE SUBJECTS AB Cluster of differentiation 36 (CD36) variants influence fasting lipids and risk of metabolic syndrome, but their impact on postprandial lipids, an independent risk factor for cardiovascular disease, is unclear. We determined the effects of SNPs within a approximate to 410 kb region encompassing CD36 and its proximal and distal promoters on chylomicron (CM) remnants and LDL particles at fasting and at 3.5 and 6 h following a high-fat meal (Genetics of Lipid Lowering Drugs and Diet Network study, n = 1,117). Five promoter variants associated with CMs, four with delayed TG clearance and five with LDL particle number. To assess mechanisms underlying the associations, we queried expression quantitative trait loci, DNA methylation, and ChIP-seq datasets for adipose and heart tissues that function in postprandial lipid clearance. Several SNPs that associated with higher serum lipids correlated with lower adipose and heart CD36 mRNA and aligned to active motifs for PPAR, a major CD36 regulator. The SNPs also associated with DNA methylation sites that related to reduced CD36 mRNA and higher serum lipids, but mixed-model analyses indicated that the SNPs and methylation independently influence CD36 mRNA. The findings support contributions of CD36 SNPs that reduce adipose and heart CD36 RNA expression to inter-individual variability of postprandial lipid metabolism and document changes in CD36 DNA methylation that influence both CD36 expression and lipids. C1 [Love-Gregory, Latisha; Abumrad, Nada A.] Washington Univ, Sch Med, Dept Med, Ctr Human Nutr, St Louis, MO 63110 USA. [Kraja, Aldi T.; Duan, Yanan; Borecki, Ingrid B.] Washington Univ, Sch Med, Dept Genet, Div Stat Genom, St Louis, MO 63110 USA. [Allum, Fiona; Grundberg, Elin] McGill Univ, Dept Human Genet, Montreal, PQ H3A 0G1, Canada. [Allum, Fiona; Grundberg, Elin] Genome Quebec Innovat Ctr, Montreal, PQ H3A 0G1, Canada. [Aslibekyan, Stella; Arnett, Donna K.] Univ Alabama Birmingham, Dept Epidemiol, Birmingham, AL 35294 USA. [Hedman, Asa K.] Uppsala Univ, Dept Med Sci, S-75185 Uppsala, Sweden. [Hedman, Asa K.] Uppsala Univ, Dept Mol Epidemiol, S-75185 Uppsala, Sweden. [Hedman, Asa K.] Uppsala Univ, Dept Sci Life Lab, S-75185 Uppsala, Sweden. [McCarthy, Mark I.] Univ Oxford, Wellcome Trust Ctr Human Genet, Oxford OX3 7BN, England. [McCarthy, Mark I.] Churchill Hosp, Oxford Ctr Diabet Endocrinol & Metab, Oxford OX3 7JU, England. [McCarthy, Mark I.] Churchill Hosp, Oxford Natl Inst Hlth Res, Biomed Res Ctr, Oxford OX3 7JU, England. [Deloukas, Panos] Queen Mary Univ London, William Harvey Res Inst, London EC1M 6BQ, England. [Ordovas, Jose M.] Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Hopkins, Paul N.] Univ Utah, Cardiovasc Genet Res, Salt Lake City, UT 84132 USA. RP Love-Gregory, L; Abumrad, NA (reprint author), Washington Univ, Sch Med, Dept Med, Ctr Human Nutr, St Louis, MO 63110 USA. EM lgregory@dom.wustl.edu; Nabumrad@wustl.edu RI Deloukas, Panos/B-2922-2013; OI Deloukas, Panos/0000-0001-9251-070X; Hedman, Asa/0000-0001-5413-204X FU Longer Life Foundation, the American Heart Association Scientist Development; Washington University Nutrition and Obesity Research Center PF [DK56341]; CTSA, National Center for Advancing Translational Sciences [UL1TR000448]; National Institute of Diabetes and Digestive and Kidney Diseases [R01 DK33301, R01 DK060022]; Cooperative State Research, Education, and Extension Service [58-1950-4-003] FX This study was supported by grants from the Longer Life Foundation, the American Heart Association Scientist Development Grant, Washington University Nutrition and Obesity Research Center P&F DK56341 and CTSA, National Center for Advancing Translational Sciences Grant UL1TR000448 (L.L-G.), National Institute of Diabetes and Digestive and Kidney Diseases Grants R01 DK33301 and R01 DK060022 (N.A.A.), and Cooperative State Research, Education, and Extension Service Grant 58-1950-4-003 (J.M.O.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. The authors declare that they have no financial conflicts of interest. NR 60 TC 1 Z9 1 U1 4 U2 4 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0022-2275 EI 1539-7262 J9 J LIPID RES JI J. Lipid Res. PD DEC PY 2016 VL 57 IS 12 BP 2176 EP 2184 DI 10.1194/jlr.P065250 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EE3FK UT WOS:000389473000008 PM 27729386 ER PT J AU Lai, CQ Wojczynski, MK Parnell, LD Hidalgo, BA Irvin, MR Aslibekyan, S Province, MA Absher, DM Arnett, DK Ordovas, JM AF Lai, Chao-Qiang Wojczynski, Mary K. Parnell, Laurence D. Hidalgo, Bertha A. Irvin, Marguerite Ryan Aslibekyan, Stella Province, Michael A. Absher, Devin M. Arnett, Donna K. Ordovas, Jose M. TI Epigenome-wide association study of triglyceride postprandial responses to a high-fat dietary challenge SO JOURNAL OF LIPID RESEARCH LA English DT Article DE DNA methylation; apolipoproteins; atherosclerosis; diet and dietary lipids; lipoproteins; triglycerides; dietary fat; postprandial lipemia ID LIPID-LOWERING DRUGS; DNA METHYLATION; HEART-DISEASE; BLOOD-LIPIDS; NONFASTING TRIGLYCERIDES; CARDIOVASCULAR-DISEASE; PLASMA TRIGLYCERIDE; WAIST CIRCUMFERENCE; OBESE SUBJECTS; RISK AB Postprandial lipemia (PPL), the increased plasma TG concentration after consuming a high-fat meal, is an independent risk factor for CVD. Individual responses to a meal high in fat vary greatly, depending on genetic and lifestyle factors. However, only a few loci have been associated with TG-PPL response. Heritable epigenomic changes may be significant contributors to the unexplained inter-individual PPL variability. We conducted an epigenome-wide association study on 979 subjects with DNA methylation measured from CD4(+) T cells, who were challenged with a high-fat meal as a part of the Genetics of Lipid Lowering Drugs and Diet Network study. Eight methylation sites encompassing five genes, LPP, CPT1A, APOA5, SREBF1, and ABCG1, were significantly associated with PPL response at an epigenome-wide level (P < 1.1 x 10(-7)), but no methylation site reached epigenome-wide significance after adjusting for baseline TG levels. Higher methylation at LPP, APOA5, SREBF1, and ABCG1, and lower methylation at CPT1A methylation were correlated with an increased TG-PPL response. These PPL-associated methylation sites, also correlated with fasting TG, account for a substantially greater amount of phenotypic variance (14.9%) in PPL and fasting TG (16.3%) when compared with the genetic contribution of loci identified by our previous genome-wide association study (4.5%). In summary, the epigenome is a large contributor to the variation in PPL, and this has the potential to be used to modulate PPL and reduce CVD. C1 [Lai, Chao-Qiang; Parnell, Laurence D.] Tufts Univ, USDA ARS, Boston, MA 02111 USA. [Ordovas, Jose M.] Tufts Univ, Nutr & Genom Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Wojczynski, Mary K.; Province, Michael A.] Washington Univ, Sch Med, Dept Genet, St Louis, MO USA. [Hidalgo, Bertha A.; Irvin, Marguerite Ryan; Aslibekyan, Stella] Univ Alabama Birmingham, Sch Publ Hlth, Dept Epidemiol, Birmingham, AL 35294 USA. [Absher, Devin M.] Hudson Alpha Inst Biotechnol, Huntsville, AL USA. [Arnett, Donna K.] Univ Kentucky, Coll Publ Hlth, Lexington, KY USA. RP Lai, CQ (reprint author), Tufts Univ, USDA ARS, Boston, MA 02111 USA. EM chaoqiang.lai@ars.usda.gov FU U.S. Department of Agriculture [8050-51000-098-00D]; National Heart, Lung, and Blood Institute [U01-HL072524-04] FX This work was funded by the U.S. Department of Agriculture under agreement number 8050-51000-098-00D and by National Heart, Lung, and Blood Institute Grant U01-HL072524-04. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the US Department of Agriculture. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 61 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0022-2275 EI 1539-7262 J9 J LIPID RES JI J. Lipid Res. PD DEC PY 2016 VL 57 IS 12 BP 2200 EP 2207 DI 10.1194/jlr.M069948 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EE3FK UT WOS:000389473000011 PM 27777315 ER PT J AU Zacek, P Bukowski, M Rosenberger, TA Picklo, M AF Zacek, Petr Bukowski, Michael Rosenberger, Thad A. Picklo, Matthew TI Quantitation of isobaric phosphatidylcholine species in human plasma using a hybrid quadrupole linear ion-trap mass spectrometer SO JOURNAL OF LIPID RESEARCH LA English DT Article DE triple quadrupole; ion-trap; National Institute of Standards and Technology human blood plasma; mass spectrometry; shotgun lipidomics; docosahexaenoic acid ID ELECTROSPRAY-IONIZATION; SHOTGUN LIPIDOMICS; HIGH-THROUGHPUT; FATTY-ACIDS; POSITIONAL-SPECIFICITY; BIOLOGICAL SAMPLES; BLOOD-PLASMA; PHOSPHOLIPIDS; LIPIDS; GLYCEROPHOSPHOLIPIDS AB Phosphatidylcholine (PC) species in human plasma are used as biomarkers of disease. PC biomarkers are often limited by the inability to separate isobaric PCs. In this work, we developed a targeted shotgun approach for analysis of isobaric and isomeric PCs. This approach is comprised of two MS methods: a precursor ion scanning (PIS) of mass m/z 184 in positive mode (PIS m/z +184) and MS3 fragmentation in negative mode, both performed on the same instrument, a hybrid triple quadrupole ion-trap mass spectrometer. The MS3 experiment identified the FA composition and the relative abundance of isobaric and sn-1, sn-2 positional isomeric PC species, which were subsequently combined with absolute quantitative data obtained by PIS m/z +184 scan. This approach was applied to the analysis of a National Institute of Standards and Technology human blood plasma standard reference material (SRM 1950). We quantified more than 70 PCs and confirmed that a majority are present in isobaric and isomeric mixtures. The FA content determined by this method was comparable to that obtained using GC with flame ionization detection, supporting the quantitative nature of this MS method. This methodology will provide more in-depth biomarker information for clinical and mechanistic studies. C1 [Zacek, Petr; Bukowski, Michael; Picklo, Matthew] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58203 USA. [Zacek, Petr] Inst Organ Chem, Prague 16610 6, Czech Republic. [Zacek, Petr] Biochem Acad Sci Czech Republ, Prague 16610 6, Czech Republic. [Rosenberger, Thad A.] Univ North Dakota, Dept Biomed Sci, Sch Med & Hlth Sci, Grand Forks, ND 58201 USA. [Picklo, Matthew] Univ North Dakota, Dept Chem, Grand Forks, ND 58201 USA. RP Zacek, P (reprint author), USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58203 USA. EM petr.zacek@ars.usda.gov FU National Institute of Food and Agriculture [2014-67017-21758]; Agricultural Research Service [5450-51000-048-00D] FX This material is based upon work that was supported by the National Institute of Food and Agriculture under Award 2014-67017-21758 and through Agricultural Research Service Project 5450-51000-048-00D. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the US Department of Agriculture. The US Department of Agriculture, Agricultural Research Service, Plains Area is an equal opportunity/affirmative action employer and all agency services are available without discrimination. Mention of trade names or commercial products in this article is solely for providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 50 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0022-2275 EI 1539-7262 J9 J LIPID RES JI J. Lipid Res. PD DEC PY 2016 VL 57 IS 12 BP 2225 EP 2234 DI 10.1194/jlr.D070656 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EE3FK UT WOS:000389473000014 PM 27688258 ER PT J AU Kieffer, DA Piccolo, BD Marco, ML Kim, EB Goodson, ML Keenan, MJ Dunn, TN Knudsen, KEB Adams, SH Martin, RJ AF Kieffer, Dorothy A. Piccolo, Brian D. Marco, Maria L. Kim, Eun Bae Goodson, Michael L. Keenan, Michael J. Dunn, Tamara N. Knudsen, Knud Erik Bach Adams, Sean H. Martin, Roy J. TI Obese Mice Fed a Diet Supplemented with Enzyme-Treated Wheat Bran Display Marked Shifts in the Liver Metabolome Concurrent with Altered Gut Bacteria SO JOURNAL OF NUTRITION LA English DT Article DE dietary fiber; gut microbiota; metabolomics; transcriptomics; xenobiotic ID CHAIN FATTY-ACIDS; INTESTINAL MICROBIOTA; RESISTANT STARCH; FIBER INTAKE; LIPID-METABOLISM; ARABINOXYLAN-OLIGOSACCHARIDES; NONALCOHOLIC STEATOHEPATITIS; AKKERMANSIA-MUCINIPHILA; FERMENTABLE FIBER; GENE-EXPRESSION AB Background: Enzyme-treated wheat bran (ETWB) contains a fermentable dietary fiber previously shown to decrease liver triglycerides (TGs) and modify the gut microbiome in mice. It is not clear which mechanisms explain how ETWB feeding affects hepatic metabolism, but factors (i.e., xenometabolites) associated with specific microbes may be involved. Objective: The objective of this study was to characterize ETWB-driven shifts in the cecal microbiome and to identify correlates between microbial changes and diet-related differences in liver metabolism in diet-induced obese mice that typically display steatosis. Methods: Five-week-old male C57BL/6J mice fed a 45%-lard based fat diet supplemented with ETWB (20% wt:wt) or rapidly digestible starch (control) (n = 15/group) for 10 wk were characterized by using a multi-omics approach. Multivariate statistical analysis was used to identify variables that were strong discriminators between the ETWB and control groups. Results: Body weight and liver TGs were decreased by ETWB feeding (by 10% and 25%, respectively; P < 0.001), and an index of liver reactive oxygen species was increased (by 29%, P < 0.01). The cecal microbiome showed an increase in Bacteroidetes (by 42%; P < 0.05) and a decrease in Firmicutes (by 16%; P < 0.05). Metabolites that were strong discriminators between the ETWB and control groups included decreased liver antioxidants (glutathione and a-tocopherol); decreased liver carbohydrate metabolites, including glucose; lower hepatic arachidonic acid; and increased liver and plasma I3-hydroxybutyrate. Liver transcriptomics revealed key metabolic pathways affected by ETWB, especially those related to lipid metabolism and some fed- or fasting-regulated genes. Conclusions: Together, these changes indicate that dietary fibers such as ETWB regulate hepatic metabolism concurrently with specific gut bacteria community shifts in C57BL/6J mice. It is proposed that these changes may elicit gut-derived signals that reach the liver via enterohepatic circulation, ultimately affecting host liver metabolism in a manner that mimics, in part, the fasting state. C1 [Kieffer, Dorothy A.; Dunn, Tamara N.; Adams, Sean H.; Martin, Roy J.] Univ Calif Davis, Grad Grp Nutr Biol, Davis, CA 95616 USA. [Kieffer, Dorothy A.; Dunn, Tamara N.; Adams, Sean H.; Martin, Roy J.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Marco, Maria L.; Kim, Eun Bae] Univ Calif Davis, Food Sci & Technol Dept, Davis, CA 95616 USA. [Goodson, Michael L.] Univ Calif Davis, Dept Microbiol, Davis, CA 95616 USA. [Kieffer, Dorothy A.; Dunn, Tamara N.; Martin, Roy J.] USDA ARS, Obes & Metab Res Unit, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [Piccolo, Brian D.; Adams, Sean H.] Univ Arkansas Med Sci, Arkansas Childrens Nutr Ctr, Little Rock, AR 72205 USA. [Piccolo, Brian D.; Adams, Sean H.] Univ Arkansas Med Sci, Dept Pediat, Little Rock, AR 72205 USA. [Kim, Eun Bae] Kangwon Natl Univ, Coll Anim Life Sci, Dept Anim Life Sci, Chunchon, Gangwon Do, South Korea. [Keenan, Michael J.] Louisiana State Univ, AgCtr, Baton Rouge, LA 70803 USA. [Knudsen, Knud Erik Bach] Aarhus Univ, Dept Anim Sci, Aarhus, Denmark. RP Adams, SH; Martin, RJ (reprint author), Univ Calif Davis, Grad Grp Nutr Biol, Davis, CA 95616 USA.; Adams, SH; Martin, RJ (reprint author), Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA.; Martin, RJ (reprint author), USDA ARS, Obes & Metab Res Unit, Western Human Nutr Res Ctr, Davis, CA 95616 USA.; Adams, SH (reprint author), Univ Arkansas Med Sci, Arkansas Childrens Nutr Ctr, Little Rock, AR 72205 USA.; Adams, SH (reprint author), Univ Arkansas Med Sci, Dept Pediat, Little Rock, AR 72205 USA. EM shadams@uams.edu; rmartin@agcenter.Isu FU T32 training award - National Center for Advancing Translational Sciences, NIH [UL1 TR000002, TL1 TF1000133]; Danish Council for Strategic Research [10-093526]; USDA-Agricultural Research Service [2032-51530-022-00D, 6026-51000-010-05S]; Arkansas Biosciences Institute, the major research component of the Tobacco Settlement Proceeds Act; NIH/National Institute of Diabetes and Digestive and Kidney Diseases [U24DK097154] FX Supported in part by a T32 training award (to DAK) funded by the National Center for Advancing Translational Sciences, NIH, through grant UL1 TR000002 and linked award TL1 TF1000133. Additional funding was provided by the Danish Council for Strategic Research Project 10-093526, USDA-Agricultural Research Service projects 2032-51530-022-00D and 6026-51000-010-05S, and in part by the Arkansas Biosciences Institute, the major research component of the Tobacco Settlement Proceeds Act of 2000. The University of California-Davis West Coast Metabolomics Center is funded by NIH/National Institute of Diabetes and Digestive and Kidney Diseases grant U24DK097154. NR 91 TC 2 Z9 2 U1 11 U2 11 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD DEC PY 2016 VL 146 IS 12 BP 2445 EP 2460 DI 10.3945/jn.116.238923 PG 16 WC Nutrition & Dietetics SC Nutrition & Dietetics GA EE2CP UT WOS:000389391600004 PM 27798344 ER PT J AU Kieffer, DA Piccolo, BD Marco, ML Kim, EB Goodson, ML Keenan, MJ Dunn, TN Knudsen, KEB Martin, RJ Adams, SH AF Kieffer, Dorothy A. Piccolo, Brian D. Marco, Maria L. Kim, Eun Bae Goodson, Michael L. Keenan, Michael J. Dunn, Tamara N. Knudsen, Knud Erik Bach Martin, Roy J. Adams, Sean H. TI Mice Fed a High-Fat Diet Supplemented with Resistant Starch Display Marked Shifts in the Liver Metabolome Concurrent with Altered Gut Bacteria SO JOURNAL OF NUTRITION LA English DT Article DE dietary fiber; gut microbiota; metabolomics; transcriptomics; liver ID CHRONIC KIDNEY-DISEASE; CHRONIC-RENAL-FAILURE; LOW-PROTEIN DIET; CYTOCHROME-P450 ENZYMES; ARACHIDONIC-ACID; DIABETES DIFFERS; LARGE-INTESTINE; BODY-FAT; MICROBIOTA; RATS AB Background: High-amylose-maize resistant starch type 2 (HAMRS2) is a fermentable dietary fiber known to alter the gut milieu, including the gut microbiota, which may explain the reported effects of resistant starch to ameliorate obesity associated metabolic dysfunction. Objective: Our working hypothesis was that HAMRS2-induced microbiome changes alter gut-derived signals (i.e., xenometabolites) reaching the liver via the portal circulation, in turn altering liver metabolism by regulating gene expression and other pathways. Methods: We used a multi-omics systems biology approach to characterize HAMRS2-driven shifts to the cecal microbiome, liver metabolome, and transcriptome, identifying correlates between microbial changes and liver metabolites under obesogenic conditions that, to our knowledge, have not previously been recognized. Five-week-old male C57BL/6J mice were fed an energy-dense 45% lard-based-fat diet for 10 wk supplemented with either 20% HAMRS2 by weight (n = 14) or rapidly digestible starch (control diet; n = 15). Results: Despite no differences in food intake, body weight, glucose tolerance, fasting plasma insulin, or liver triglycerides, the HAMRS2 mice showed a 15-58% reduction in all measured liver amino acids, except for Gln, compared with control mice. These metabolites were equivalent in the plasma of HAMRS2 mice compared with controls, and transcripts encoding key amino acid transporters were not different in the small intestine or liver, suggesting that HAMRS2 effects were not simply due to lower hepatocyte exposure to systemic amino acids. Instead, alterations in gut microbial metabolism could have affected host nitrogen and amino acid homeostasis: HAMRS2 mice showed a 62% increase (P < 0.0001) in 48-h fecal output and a 41% increase (P < 0.0001) in fecal nitrogen compared with control mice. Beyond amino acid metabolism, liver transcriptomics revealed pathways related to lipid and xenobiotic metabolism; and pathways related to cell proliferation, differentiation, and growth were affected by HAMRS2 feeding. Conclusion: Together, these differences indicate that HAMRS2 dramatically alters hepatic metabolism and gene expression concurrent with shifts in specific gut bacteria in C57BL/6J mice. C1 [Kieffer, Dorothy A.; Dunn, Tamara N.; Martin, Roy J.; Adams, Sean H.] Univ Calif Davis, Grad Grp Nutr Biol, Davis, CA 95616 USA. [Kieffer, Dorothy A.; Dunn, Tamara N.; Martin, Roy J.; Adams, Sean H.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Marco, Maria L.; Kim, Eun Bae] Univ Calif Davis, Food Sci & Technol Dept, Davis, CA 95616 USA. [Goodson, Michael L.] Univ Calif Davis, Dept Microbiol, Davis, CA 95616 USA. [Kieffer, Dorothy A.; Dunn, Tamara N.; Martin, Roy J.] USDA ARS, Obes & Metab Res Unit, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [Piccolo, Brian D.; Adams, Sean H.] Univ Arkansas Med Sci, Arkansas Childrens Nutr Ctr, Little Rock, AR 72205 USA. [Piccolo, Brian D.; Adams, Sean H.] Univ Arkansas Med Sci, Dept Pediat, Little Rock, AR 72205 USA. [Kim, Eun Bae] Kangwon Natl Univ, Coll Anim Life Sci, Dept Anim Life Sci, Chunchon, Gangwon Do, South Korea. [Keenan, Michael J.] Louisiana State Univ, AgCtr, Baton Rouge, LA 70803 USA. [Knudsen, Knud Erik Bach] Aarhus Univ, Dept Anim Sci, Aarhus, Denmark. RP Martin, RJ; Adams, SH (reprint author), Univ Calif Davis, Grad Grp Nutr Biol, Davis, CA 95616 USA.; Martin, RJ; Adams, SH (reprint author), Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA.; Martin, RJ (reprint author), USDA ARS, Obes & Metab Res Unit, Western Human Nutr Res Ctr, Davis, CA 95616 USA.; Adams, SH (reprint author), Univ Arkansas Med Sci, Arkansas Childrens Nutr Ctr, Little Rock, AR 72205 USA.; Adams, SH (reprint author), Univ Arkansas Med Sci, Dept Pediat, Little Rock, AR 72205 USA. EM rmartin@agcenter.lsu.edu; shadams@uams.edu FU National Center for Advancing Translational Sciences, NIH [UL1 TR000002, TL1 TR000133]; Danish Council for Strategic Research [10-093526]; USDA-Agricultural Research Service projects [2032-51530-022-00D, 6026-51000-010-05S]; Arkansas Biosciences Institute, the major research component of the Tobacco Settlement Proceeds Act; NIH/National Institute of Diabetes and Digestive and Kidney Diseases [U24DK097154] FX Supported in part by a T32 training award (to DAK) funded by the National Center for Advancing Translational Sciences, NIH, through grant UL1 TR000002 and linked award TL1 TR000133. Additional funding was provided by the Danish Council for Strategic Research Project 10-093526, USDA-Agricultural Research Service projects 2032-51530-022-00D and 6026-51000-010-05S, and in part by the Arkansas Biosciences Institute, the major research component of the Tobacco Settlement Proceeds Act of 2000. The University of California-Davis West Coast Metabolomics Center is funded by NIH/National Institute of Diabetes and Digestive and Kidney Diseases grant U24DK097154. NR 67 TC 1 Z9 1 U1 19 U2 19 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD DEC PY 2016 VL 146 IS 12 BP 2476 EP 2490 DI 10.3945/jn.116.238931 PG 15 WC Nutrition & Dietetics SC Nutrition & Dietetics GA EE2CP UT WOS:000389391600007 PM 27807042 ER PT J AU Yu, SG Wu, XL Ferguson, M Simmen, RCM Cleves, MA Simmen, FA Fang, NB AF Yu, Shanggong Wu, Xianli Ferguson, Matthew Simmen, Rosalia C. M. Cleves, Mario A. Simmen, Frank A. Fang, Nianbai TI Diets Containing Shiitake Mushroom Reduce Serum Lipids and Serum Lipophilic Antioxidant Capacity in Rats SO JOURNAL OF NUTRITION LA English DT Article DE rat; shiitake; antioxidant capacity; leptin; insulin; sex differences ID LENTINUS-EDODES; PLASMA LEPTIN; NORMAL-WEIGHT; FRUIT BODIES; CONSUMPTION; HUMANS; WOMEN; OBESE; FOODS; MICE AB Background: We previously reported that dietary intake of shiitake mushroom (SM; Lentinus edodes) decreased serum concentrations of polar lipids in male rats. Objective: This study evaluated the dietary effects of SM on serum cholesterol related and serum antioxidant indexes in rats of both sexes. Methods: Sprague-Dawley rats [38 dams and their offspring (20 males and 20 females/diet)] were fed diets containing 0 (control), 1%, 4%, or 10% (wt:wt) SM powder from gestation day 4 through to postnatal day (PND) 126. Biochemical indexes were monitored during the midgrowth phase (PNDs 50-66). Results: The food consumption by offspring fed the control diet and diets supplemented with SM was not different when measured on PND 65. However, the 4% and 10% SM diets resulted in male rats with 7% lower body weights than those of the other 2 groups on PND 66. SM consumption dose-dependently decreased the concentrations of lipidemia-related factors in sera, irrespective of sex. At PND 50, serum concentrations of total cholesterol, HDL cholesterol, and non-HDL cholesterol in SM-fed male and female rats were generally lower (3-27%) than those in the corresponding control groups. Consumption of the 10% SM diet resulted in significantly decreased (55%) serum triglyceride concentrations relative to the control groups for both sexes. The 10% SM diet elicited a 62% reduction of serum leptin concentrations in females but not in males, and this same diet increased serum insulin (137%) and decreased serum glucose (15%) in males compared with controls. Serum lipophilic antioxidant capacity in males and females fed SM diets was generally lower (31-86%) than that in the control groups. Conclusion: SM decreased the concentrations of lipidemia-related factors in rat sera irrespective of sex. The SM-elicited reduction of lipophilic antioxidant capacity irrespective of sex may reflect a lower pro-oxidative state and, hence, improved metabolic profile. C1 [Yu, Shanggong; Ferguson, Matthew; Fang, Nianbai] Arkansas Childrens Nutr Ctr, Little Rock, AR 72202 USA. [Wu, Xianli] USDA ARS, Beltsville Human Nutr Res Ctr, Beltsville, MD USA. [Simmen, Rosalia C. M.; Simmen, Frank A.] Univ Arkansas Med Sci, Dept Physiol & Biophys, Little Rock, AR 72205 USA. [Cleves, Mario A.; Fang, Nianbai] Univ Arkansas Med Sci, Dept Pediat, Little Rock, AR 72205 USA. RP Fang, NB (reprint author), Arkansas Childrens Nutr Ctr, Little Rock, AR 72202 USA.; Simmen, FA (reprint author), Univ Arkansas Med Sci, Dept Physiol & Biophys, Little Rock, AR 72205 USA.; Fang, NB (reprint author), Univ Arkansas Med Sci, Dept Pediat, Little Rock, AR 72205 USA. EM simmenfranka@uams.edu; NFang@uams.edu FU USDA Agriculture Research Service Project [6251-51000-005-02S]; NIH/National Cancer Institute [MBS-95016-13] FX Supported by USDA Agriculture Research Service Project 6251-51000-005-02S and NIH/National Cancer Institute grant MBS-95016-13. NR 38 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD DEC PY 2016 VL 146 IS 12 BP 2491 EP 2496 DI 10.3945/jn.116.239806 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA EE2CP UT WOS:000389391600008 PM 27798348 ER PT J AU Zhang, FF Ojha, RP Krull, KR Gibson, TM Lu, L Lanctot, J Chemaitilly, W Robison, LL Hudson, MM AF Zhang, Fang Fang Ojha, Rohit P. Krull, Kevin R. Gibson, Todd M. Lu, Lu Lanctot, Jennifer Chemaitilly, Wassim Robison, Leslie L. Hudson, Melissa M. TI Adult Survivors of Childhood Cancer Have Poor Adherence to Dietary Guidelines SO JOURNAL OF NUTRITION LA English DT Article DE diet quality; nutrition; childhood cancer survivors; cancer survivors; cancer treatment ID ACUTE LYMPHOBLASTIC-LEUKEMIA; PHYSICAL-ACTIVITY; 5-YEAR SURVIVORS; LATE MORTALITY; BREAST-CANCER; RISK-FACTORS; QUALITY; THERAPY; HEALTH; QUESTIONNAIRES AB Background: Poor nutritional intake can exacerbate the chronic disease burden in childhood cancer survivors, whereas a healthful diet serves a protective function. Few studies have provided detailed evaluations of the diet of childhood cancer survivors. Objectives: This study aimed to evaluate diet quality and dietary intakes of key food groups and nutrients in a large cohort of childhood cancer survivors and whether cancer and treatment characteristics have an impact on survivors' long-term intake. Methods: Diet was assessed in 2570 adult survivors of childhood cancer enrolled in the St. Jude Lifetime cohort (mean age = 32.3 y) by using the Block food-frequency questionnaire. The Healthy Eating Index-2010 (HEI-2010) was calculated to quantify diet quality. Cancer diagnosis and treatment exposure were abstracted from medical records. Differences in HEI-2010 by patient characteristics and treatment exposure were examined by using ANCOVA. Results: The mean +/- SD HEI-2010 in childhood cancer survivors was 57.9 +/- 12.4 of a maximum score of 100. Referenced to Dietary Reference Intakes, survivors consumed inadequate amounts of vitamin D, vitamin E, potassium, fiber, magnesium, and calcium (27%, 54%, 58%, 59%, 84%, and 90% of the recommended intakes) but excessive amounts of sodium and saturated fat (155% and 115% of the recommended intakes) from foods. Survivors diagnosed when <5 y of age had a lower diet quality than did those diagnosed when >= 5 y of age (mean HEI-2010 score: 56.9 compared with 58.2; P = 0.046). Survivors who received higher radiation doses to the abdomen had a lower diet quality than those who received lower doses (mean HEI-2010 scores = 58.9, 57.2, 56.7, and 56.1 for doses of 0, 1-19.9, 20-29.9, and >= 30 Gy, respectively; P = 0.02). Conclusions: Long-term childhood cancer survivors have poor adherence to the 2010 Dietary Guidelines for Americans. Findings reinforce the need to incorporate nutrition into cancer care to improve diet quality and to reduce morbidities. C1 [Zhang, Fang Fang] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Zhang, Fang Fang] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr, Boston, MA 02111 USA. [Ojha, Rohit P.; Krull, Kevin R.; Gibson, Todd M.; Lu, Lu; Lanctot, Jennifer; Robison, Leslie L.; Hudson, Melissa M.] St Jude Childrens Res Hosp, Dept Epidemiol & Canc Control, 332 N Lauderdale St, Memphis, TN 38105 USA. [Chemaitilly, Wassim] St Jude Childrens Res Hosp, Dept Endocrinol, 332 N Lauderdale St, Memphis, TN 38105 USA. [Hudson, Melissa M.] St Jude Childrens Res Hosp, Dept Oncol, 332 N Lauderdale St, Memphis, TN 38105 USA. RP Zhang, FF (reprint author), Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA.; Zhang, FF (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr, Boston, MA 02111 USA. EM fang_fang.zhang@tufts.edu OI Ojha, Rohit/0000-0003-0595-8990 FU NIH/National Cancer Institute (NIH/NCI) [1R03CA199516-01]; NIH/NCI [U01CA194457]; American Lebanese Syrian Associated Charities FX Supported by NIH/National Cancer Institute (NIH/NCI) 1R03CA199516-01 (FFZ), NIH/NCI U01CA194457 (MMH), and American Lebanese Syrian Associated Charities (RPO, KRK, TMG, JL, WC, LLR, and MMH). NR 52 TC 0 Z9 0 U1 21 U2 21 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD DEC PY 2016 VL 146 IS 12 BP 2497 EP 2505 DI 10.3945/jn.116.238261 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA EE2CP UT WOS:000389391600009 PM 27798341 ER PT J AU Ma, JT Jacques, PF Meigs, JB Fox, CS Rogers, GT Smith, CE Hruby, A Saltzman, E McKeown, NM AF Ma, Jiantao Jacques, Paul F. Meigs, James B. Fox, Caroline S. Rogers, Gail T. Smith, Caren E. Hruby, Adela Saltzman, Edward McKeown, Nicola M. TI Sugar-Sweetened Beverage but Not Diet Soda Consumption Is Positively Associated with Progression of Insulin Resistance and Prediabetes SO JOURNAL OF NUTRITION LA English DT Article DE sugar-sweetened beverages; diet soda; insulin resistance; HOMA-IR; prediabetes ID CARDIOMETABOLIC RISK-FACTORS; RANDOMIZED CONTROLLED-TRIAL; SOFT DRINK CONSUMPTION; CORONARY-HEART-DISEASE; METABOLIC SYNDROME; CARDIOVASCULAR-DISEASE; GLYCEMIC INDEX; AGED ADULTS; FRUIT JUICE; MEN AB Background: Previous studies have shown an inconsistent relation between habitual beverage consumption and insulin resistance and prediabetes. Objective: The objective of the present study was to test the hypothesis that the consumption of sugar-sweetened beverages (SSBs), rather than diet soda, is associated with long-term progression of insulin resistance and the development of prediabetes. Methods: We analyzed the prospective association between cumulative mean consumption of SSBs or diet soda and incident prediabetes (n = 1685) identified across a median of 14 y of follow-up in participants [mean +/- SD age: 51.9 +/- 9.2 y; 59.6% women; mean +/- SD body mass index (BMI; kg/m(2)): 26.3 +/- 4.4] of the Framingham Offspring cohort. The prospective association between beverage consumption and change in homeostasis model assessment of insulin resistance (HOMA-IR; n = 2076) over 7 y was also analyzed. The cumulative mean consumption of SSBs and diet soda was estimated by using food-frequency questionnaires. Multivariable Cox proportional hazards models and linear regression models were implemented to estimate the HRs of incident prediabetes and change in HOMA-IR, respectively. Results: After adjustment for multiple potential confounders, including baseline BMI, we observed that SSB intake was positively associated with incident prediabetes (P-trend < 0.001); the highest SSB consumers (>3 servings/wk; median: 6 servings/wk) had a 46% higher risk of developing prediabetes than did the SSB nonconsumers (HR: 1.46; 95% CI: 1.16, 1.83). Higher SSB intake was also associated with a greater increase in HOMA-IR (P-trend = 0.006). No prospective associations were observed between diet soda intake and risk of prediabetes (P-trend = 0.24) or changes in HOMA-IR (P-trend = 0.25). These associations were similar after additional adjustment for change in BMI. Conclusion: Regular SSB intake, but not diet soda intake, is associated with a greater increase in insulin resistance and a higher risk of developing prediabetes in a group of middle-aged adults. C1 [Ma, Jiantao; Jacques, Paul F.; Rogers, Gail T.; Hruby, Adela; McKeown, Nicola M.] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Nutr Epidemiol Lab, Boston, MA 02111 USA. [Smith, Caren E.] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA. [Saltzman, Edward] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Energy Metab Lab, Boston, MA 02111 USA. [Meigs, James B.] Harvard Med Sch, Dept Med, Div Gen Internal Med, Boston, MA USA. [Meigs, James B.] Massachusetts Gen Hosp, Boston, MA 02114 USA. [Fox, Caroline S.] NHLBI, Framingham Heart Study, Populat Sci Branch, Framingham, MA USA. RP McKeown, NM (reprint author), Tufts Univ, USDA, Human Nutr Res Ctr Aging, Nutr Epidemiol Lab, Boston, MA 02111 USA. EM nicola.mckeown@tufts.edu FU NIH/National Heart, Lung, and Blood Institute's Framingham Heart Study [N01-HC-25195]; Boston University School of Medicine; USDA (USDA) [58-1950-0-014] FX Supported by the NIH/National Heart, Lung, and Blood Institute's Framingham Heart Study (contract N01-HC-25195), the Boston University School of Medicine, and the USDA (USDA agreement 58-1950-0-014). NR 38 TC 0 Z9 0 U1 5 U2 5 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD DEC PY 2016 VL 146 IS 12 BP 2544 EP 2550 DI 10.3945/jn.116.234047 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA EE2CP UT WOS:000389391600015 PM 27934644 ER PT J AU Cynober, L Bier, DM Kadowaki, M Morris, SM Elangoio, R Smriga, M AF Cynober, Luc Bier, Dennis M. Kadowaki, Motoni Morris, Sidney M., Jr. Elangoio, Rajavel Smriga, Miro TI Proposals for Upper Limits of Safe Intake for Arginine and Tryptophan in Young Adults and an Upper Limit of Safe Intake for Leucine in the Elderly SO JOURNAL OF NUTRITION LA English DT Article; Proceedings Paper CT 9th Workshop on the Assessment of Adequate and Safe Intake of Dietary Amino Acids at the 9th Amino Acid Assessment Workshop CY OCT 15-16, 2015 CL Paris, FRANCE SP Int Council Amino Acid Sci DE arginine; leucine; tryptophan; NOAEL; safe intake ID SUPPLEMENTATION; EXERCISE; SORENESS; HUMANS; MOOD AB On the basis of research presented during the 9th Amino Acid Assessment Workshop, a No Observed Adverse Effect Level (NOAEL) for diet-added arginine (added mostly in the form of dietary supplements) of 30 g/d and an upper limit of safe intake (ULSI) for diet-added tryptophan (added mostly in the form of dietary supplements) of 4.5 g/d have been proposed. Both recommendations apply to healthy young adults. The total dietary leucine ULSI proposed for elderly individuals is 500 mg . kg(-1) . d(-1). All 3 recommendations are relevant only to high-quality amino acid-containing products with specifications corresponding to those listed in the US Pharmacopeia. Because the above amino acids are extensively utilized as dietary supplements for various real or perceived benefits, such as vasodilation, spermatogenesis, sleep, mood regulation, or muscle recovery, the above safety recommendations will have an important impact on regulatory and nutritional practices. C1 [Cynober, Luc] Cochin Hosp, AP HP, Clin Chem Lab, Paris, France. [Cynober, Luc] Paris Descartes Univ, Fac Pharm, Biol Nutr Lab, Paris, France. [Cynober, Luc] Paris Descartes Univ, Fac Pharm, EA 4466, Paris, France. [Bier, Dennis M.] Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Houston, TX 77030 USA. [Kadowaki, Motoni] Niigata Univ, Grad Sch Sci & Technol, Niigata, Japan. [Morris, Sidney M., Jr.] Univ Pittsburgh, Sch Med, Pittsburgh, PA USA. [Elangoio, Rajavel] Univ British Columbia, Sch Populat & Publ Hlth, Dept Pediat, Vancouver, BC, Canada. [Cynober, Luc; Bier, Dennis M.; Kadowaki, Motoni; Morris, Sidney M., Jr.; Elangoio, Rajavel] Int Council Amino Acid Sci, Sci Advisory Comm, Brussels, Belgium. [Smriga, Miro] Int Council Amino Acid Sci, Brussels, Belgium. RP Cynober, L (reprint author), Cochin Hosp, AP HP, Clin Chem Lab, Paris, France.; Cynober, L (reprint author), Paris Descartes Univ, Fac Pharm, Biol Nutr Lab, Paris, France.; Cynober, L (reprint author), Paris Descartes Univ, Fac Pharm, EA 4466, Paris, France.; Cynober, L (reprint author), Int Council Amino Acid Sci, Sci Advisory Comm, Brussels, Belgium. EM luc.cynober@aphp.fr OI Elango, Rajavel/0000-0002-9380-1725 NR 17 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD DEC PY 2016 VL 146 IS 12 BP 2652 EP 2654 DI 10.3945/jn.115.228478 PG 3 WC Nutrition & Dietetics SC Nutrition & Dietetics GA EE2CP UT WOS:000389391600030 PM 27934658 ER PT J AU Safeeq, M Hunsaker, CT AF Safeeq, Mohammad Hunsaker, Carolyn T. TI CHARACTERIZING RUNOFF AND WATER YIELD FOR HEADWATER CATCHMENTS IN THE SOUTHERN SIERRA NEVADA SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE Sierra Nevada; water yield; normalized difference vegetation index; evapotranspiration ID WESTERN UNITED-STATES; PACIFIC-NORTHWEST; RECESSION ANALYSIS; CLIMATE-CHANGE; STREAMFLOW RESPONSE; HYDROLOGIC RESPONSE; PRECIPITATION; BASEFLOW; AMERICA; SENSITIVITY AB In a Mediterranean climate where much of the precipitation falls during winter, snowpacks serve as the primary source of dry season runoff. Increased warming has led to significant changes in hydrology of the western United States. An important question in this context is how to best manage forested catchments for water and other ecosystem services? Answering this basic question requires detailed understanding of hydrologic functioning of these catchments. Here, we depict the differences in hydrologic response of 10 catchments. Size of the study catchments ranges from 50 to 475 ha, and they span between 1,782 and 2,373 m elevation in the rain-snow transitional zone. Mean annual streamflow ranged from 281 to 408 mm in the low elevation Providence and 436 to 656 mm in the high elevation Bull catchments, resulting in a 49 mm streamflow increase per 100 m (R-2 = 0.79) elevation gain, despite similar precipitation across the 10 catchments. Although high elevation Bull catchments received significantly more precipitation as snow and thus experienced a delayed melt, this increase in streamflow with elevation was mainly due to a reduction in evapotranspiration (ET) with elevation (45 mm/100 m, R-2 = 0.65). The reduction in ET was attributed to decline in vegetation density, growing season, and atmospheric demand with increasing elevation. These findings suggest changes in streamflow in response to climate warming may likely depend on how vegetation responds to those changes in climate. C1 [Safeeq, Mohammad] Univ Calif, Sierra Nevada Res Inst, 5200 N Lake Rd, Merced, CA 95343 USA. [Hunsaker, Carolyn T.] US Forest Serv, Pacific Southwest Res Stn, Fresno, CA 93710 USA. RP Safeeq, M (reprint author), Univ Calif, Sierra Nevada Res Inst, 5200 N Lake Rd, Merced, CA 95343 USA. EM msafeeq@ucmerced.edu FU National Fire Plan; Pacific Southwest Research Station; Southern Sierra Critical Zone Observatory (National Science Foundation) [EAR-0725097] FX Many people have been critical to the success of this long-term research so we can't possibly mention everyone here. Sean Eagan and Jeff Anderson were the hydrologists who designed and installed most instruments at KREW; Thomas Whitaker was the hydrologist who helped Dr. Hunsaker run KREW after Mr. Eagan left; and more recently KREW has been kept functional by Kevin Mazzocco and Amber Olsson (PSW technicians) and Jason Smith (UC Merced technician). Matt Stuemky is appreciated for his GIS and database work over the years. KREW was established with USDA Forest Service funding from the National Fire Plan and consistently supported by the Pacific Southwest Research Station. Some support since 2007 also has been provided through the Southern Sierra Critical Zone Observatory (National Science Foundation EAR-0725097). Use of firm or trade names is for reader information only and does not imply endorsement of any product or service by the U.S. Government. NR 75 TC 0 Z9 0 U1 10 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD DEC PY 2016 VL 52 IS 6 BP 1327 EP 1346 DI 10.1111/1752-1688.12457 PG 20 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA EE0YR UT WOS:000389307000004 ER PT J AU Coats, R Lewis, J Alvarez, N Arneson, P AF Coats, Robert Lewis, Jack Alvarez, Nancy Arneson, Patricia TI TEMPORAL AND SPATIAL TRENDS IN NUTRIENT AND SEDIMENT LOADING TO LAKE TAHOE, CALIFORNIA-NEVADA, USA SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE biogeochemistry; environmental impacts; lakes; rivers/streams; monitoring; statistics; environmental sampling; nutrients; sediment; eutrophication; total load ID UNITED-STATES; SUSPENDED SEDIMENT; NITROGEN FLUXES; LAND-USE; BASIN; TRANSPORT; STREAMS; PHOSPHORUS; REGRESSION; ECOSYSTEMS AB Since 1980, the Lake Tahoe Interagency Monitoring Program (LTIMP) has provided streamdischarge and water quality data-nitrogen (N), phosphorus (P), and suspended sediment-at more than 20 stations in Lake Tahoe Basin streams. To characterize the temporal and spatial patterns in nutrient and sediment loading to the lake, and improve the usefulness of the program and the existing database, we have (1) identified and corrected for sources of bias in the water quality database; (2) generated synthetic datasets for sediments and nutrients, and resampled to compare the accuracy and precision of different load calculation models; (3) using the best models, recalculated total annual loads over the period of record; (4) regressed total loads against total annual and annual maximum daily discharge, and tested for time trends in the residuals; (5) compared loads for different forms of N and P; and (6) tested constituent loads against land use-land cover (LULC) variables using multiple regression. The results show (1) N and P loads are dominated by organic N and particulate P; (2) there are significant long-term downward trends in some constituent loads of some streams; and (3) anthropogenic impervious surface is the most important LULC variable influencing water quality in basin streams. Many of our recommendations for changes in water quality monitoring and load calculation methods have been adopted by the LTIMP. C1 [Coats, Robert] Univ Calif Davis, Dept Environm Sci & Policy, 2512 9th St,Ste 7, Berkeley, CA 94710 USA. [Lewis, Jack] US Forest Serv, Pacific Southwest Res Stn, USDA, Arcata, CA 95521 USA. [Alvarez, Nancy] US Geol Survey, Water Resource Div, Carson City, NV 89701 USA. [Arneson, Patricia] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. RP Coats, R (reprint author), Univ Calif Davis, Dept Environm Sci & Policy, 2512 9th St,Ste 7, Berkeley, CA 94710 USA. EM rncoats@ucdavis.edu FU USDA Forest Service under the Southern Nevada Public Land Management Act [13-DG-11272170-009] FX We thank Kip Allander, David Berger, John Reuter, and Andrew Stubblefield for review of the manuscript; Nicole Beck, Gary Conley, Mark Grismer, Alan Heyvaert, Andrew Stubblefield, and Rick Susfalk for generously sharing their turbidity and discharge data for basin streams; Scott Hackley for dedicated stream sampling at all hours and in all weather; Dan Nover for sharing his fine sediment data; John Reuter, Earl Byron, and Rich Axler for directing the LTIMP in years past; Charles Goldman and Geoffrey Schladow for their leadership of UC Davis research program (spanning over five decades) in the Tahoe Basin; Melissa Kimble, Humboldt State Univ. and Jim Menlove of the Forest Service Rocky Mt. Res. Sta. for providing the Forest Inventory and Analysis data for the Tahoe Basin; and Tiff van Huysen, Zach Hymanson, and George Malyj for administrative support. We humbly acknowledge the contribution of the late Robert Leonard, who was instrumental in organizing and shepherding the LTIMP through its early years. This work was funded by grant no. 13-DG-11272170-009 from USDA Forest Service under the Southern Nevada Public Land Management Act. NR 73 TC 0 Z9 0 U1 23 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD DEC PY 2016 VL 52 IS 6 BP 1347 EP 1365 DI 10.1111/1752-1688.12461 PG 19 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA EE0YR UT WOS:000389307000005 ER PT J AU Bieger, K Rathjens, H Arnold, JG Chaubey, I Allen, PM AF Bieger, Katrin Rathjens, Hendrik Arnold, Jeffrey G. Chaubey, Indrajeet Allen, Peter M. TI DEVELOPMENT AND COMPARISON OF MULTIPLE REGRESSION MODELS TO PREDICT BANKFULL CHANNEL DIMENSIONS FOR USE IN HYDROLOGIC MODELS SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE rivers/streams; watersheds; geomorphology; channel geometry; multiple regression; nationwide and regional models; hydrologic modeling ID HYDRAULIC GEOMETRY RELATIONSHIPS; SPATIAL AUTOCORRELATION; RECURRENCE INTERVALS; ALLUVIAL CHANNELS; NORTH-CAROLINA; UNITED-STATES; STREAMS; RIVERS; URBAN; WIDTH AB Channel dimensions are important input variables for many hydrologic models. As measurements of channel geometry are not available in most watersheds, they are often predicted using bankfull hydraulic geometry relationships. This study aims at improving existing equations that relate bankfull width, depth, and cross-sectional area to drainage area (DA) without limiting their use to well-gauged watersheds. We included seven additional variables in the equations that can be derived from data that are generally required by hydrologic models anyway and conducted several multiple regression analyses to identify the ideal combination of additional variables for nationwide and regional models for each Physiographic Division of the United States (U.S.). Results indicate that including the additional variables in the regression equations generally improves predictions considerably. The selection of relevant variables varies by Physiographic Division, but average annual precipitation (PCP) and temperature (TMP) were generally found to improve the models the most. Therefore, we recommend using regression equations with three independent variables (DA, PCP, and TMP) to predict bankfull channel dimensions for hydrologic models. Furthermore, we recommend using the regional equations for watersheds within regions from which data were used for model development, whereas in all other parts of the U.S. and the rest of the world, the nationwide equations should be given preference. C1 [Bieger, Katrin] Texas A&M AgriLife, Blackland Res & Extens Ctr, 720 E Blackland Rd, Temple, TX 76502 USA. [Rathjens, Hendrik; Chaubey, Indrajeet] Purdue Univ, Dept Earth Atmospher & Planetary Sci, W Lafayette, IN 47907 USA. [Arnold, Jeffrey G.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Allen, Peter M.] Baylor Univ, Dept Geol, Waco, TX 76798 USA. RP Bieger, K (reprint author), Texas A&M AgriLife, Blackland Res & Extens Ctr, 720 E Blackland Rd, Temple, TX 76502 USA. EM kbieger@brc.tamus.edu NR 59 TC 0 Z9 0 U1 1 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD DEC PY 2016 VL 52 IS 6 BP 1385 EP 1400 DI 10.1111/1752-1688.12460 PG 16 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA EE0YR UT WOS:000389307000007 ER PT J AU Tatineni, S French, R AF Tatineni, Satyanarayana French, Roy TI The Coat Protein and NIa Protease of Two Potyviridae Family Members Independently Confer Superinfection Exclusion SO JOURNAL OF VIROLOGY LA English DT Article ID STREAK-MOSAIC-VIRUS; CITRUS-TRISTEZA-VIRUS; HEPATITIS-C VIRUS; ERIOPHYID MITE TRANSMISSION; VESICULAR STOMATITIS-VIRUS; LONG-DISTANCE TRANSPORT; SEMLIKI-FOREST-VIRUS; IN-VITRO TRANSCRIPTS; WHEAT CURL MITE; CROSS-PROTECTION AB Superinfection exclusion (SIE) is an antagonistic virus-virus interaction whereby initial infection by one virus prevents subsequent infection by closely related viruses. Although SIE has been described in diverse viruses infecting plants, humans, and animals, its mechanisms, including involvement of specific viral determinants, are just beginning to be elucidated. In this study, SIE determinants encoded by two economically important wheat viruses, Wheat streak mosaic virus (WSMV; genus Tritimovirus, family Potyviridae) and Triticum mosaic virus (TriMV; genus Poacevirus, family Potyviridae), were identified in gain-of-function experiments that used heterologous viruses to express individual virus-encoded proteins in wheat. Wheat plants infected with TriMV expressing WSMV P1, HC-Pro, P3, 6K1, CI, 6K2, NIa-VPg, or NIb cistrons permitted efficient superinfection by WSMV expressing green fluorescent protein (WSMV-GFP). In contrast, wheat infected with TriMV expressing WSMV NIa-Pro or coat protein (CP) substantially excluded superinfection by WSMV-GFP, suggesting that both of these cistrons are SIE effectors encoded by WSMV. Importantly, SIE is due to functional WSMV NIa-Pro or CP rather than their encoding RNAs, as altering the coded protein products by minimally changing RNA sequences led to abolishment of SIE. Deletion mutagenesis further revealed that elicitation of SIE by NIa-Pro requires the entire protein while CP requires only a 200-amino-acid (aa) middle fragment (aa 101 to 300) of the 349 aa. Strikingly, reciprocal experiments with WSMV-mediated expression of TriMV proteins showed that TriMV CP, and TriMV NIa-Pro to a lesser extent, likewise excluded superinfection by TriMV-GFP. Collectively, these data demonstrate that WSMV-and TriMV-encoded CP and NIa-Pro proteins are effectors of SIE and that these two proteins trigger SIE independently of each other. IMPORTANCE Superinfection exclusion (SIE) is an antagonistic virus-virus interaction that prevents secondary invasions by identical or closely related viruses in the same host cells. Although known to occur in diverse viruses, SIE remains an enigma in terms of key molecular determinants and action mechanisms. In this study, we found that Wheat streak mosaic virus (WSMV) and Triticum mosaic virus (TriMV) encode two independently functioning cistrons that serve as effectors of SIE at the protein but not the RNA level. The coat protein and NIa-Pro encoded by these two viruses, when expressed from a heterologous virus, exerted SIE to the cognate viruses. The identification of virus-encoded effectors of SIE and their transgenic expression could potentially facilitate the development of virus-resistant crop plants. Additionally, functional conservation of SIE in diverse virus groups suggests that a better understanding of the underlying mechanisms of SIE could facilitate the development of novel antiviral therapies against viral diseases. C1 [Tatineni, Satyanarayana] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. RP Tatineni, S (reprint author), Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. EM satya.tatineni@ars.usda.gov FU USDA ARS CRIS [5440-21000-031-00D]; USDA \ National Institute of Food and Agriculture (NIFA) [2013-68004-20358] FX This work was funded by USDA ARS CRIS (5440-21000-031-00D). This work was also funded, in part, by USDA vertical bar National Institute of Food and Agriculture (NIFA) (2013-68004-20358). NR 81 TC 0 Z9 0 U1 8 U2 8 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD DEC PY 2016 VL 90 IS 23 BP 10886 EP 10905 DI 10.1128/JVI.01697-16 PG 20 WC Virology SC Virology GA EE8VL UT WOS:000389904500037 PM 27681136 ER PT J AU Nelson, MI Stucker, KM Schobel, SA Trovao, NS Das, SR Dugan, VG Nelson, SW Sreevatsan, S Killian, ML Nolting, JM Wentworth, DE Bowman, AS AF Nelson, Martha I. Stucker, Karla M. Schobel, Seth A. Trovao, Nidia S. Das, Suman R. Dugan, Vivien G. Nelson, Sarah W. Sreevatsan, Srinand Killian, Mary L. Nolting, Jacqueline M. Wentworth, David E. Bowman, Andrew S. TI Introduction, Evolution, and Dissemination of Influenza A Viruses in Exhibition Swine in the United States during 2009 to 2013 SO JOURNAL OF VIROLOGY LA English DT Article ID DYNAMICS; HUMANS AB The swine-human interface created at agricultural fairs, along with the generation of and maintenance of influenza A virus diversity in exhibition swine, presents an ongoing threat to public health. Nucleotide sequences of influenza A virus isolates collected from exhibition swine in Ohio (n = 262) and Indiana (n = 103) during 2009 to 2013 were used to investigate viral evolution and movement within this niche sector of the swine industry. Phylogenetic and Bayesian analyses were employed to identify introductions of influenza A virus to exhibition swine and study viral population dynamics. In 2013 alone, we identified 10 independent introductions of influenza A virus into Ohio and/or Indiana exhibition swine. Frequently, viruses from the same introduction were identified at multiple fairs within the region, providing evidence of rapid and widespread viral movement within the exhibition swine populations of the two states. While pigs moving from fair to fair to fair is possible in some locations, the concurrent detection of nearly identical strains at several fairs indicates that a common viral source was more likely. Importantly, we detected an association between the high number of human variant H3N2 (H3N2v) virus infections in 2012 and the widespread circulation of influenza A viruses of the same genotype in exhibition swine in Ohio fairs sampled that year. The extent of viral diversity observed in exhibition swine and the rapidity with which it disseminated across long distances indicate that novel strains of influenza A virus will continue to emerge and spread within exhibition swine populations, presenting an ongoing threat to humans. IMPORTANCE Understanding the underlying population dynamics of influenza A viruses in commercial and exhibition swine is central to assessing the risk for human infections with variant viruses, including H3N2v. We used viral genomic sequences from isolates collected from exhibition swine during 2009 to 2013 to understand how the peak of H3N2v cases in 2012 relates to long-term trends in the population dynamics of pandemic viruses recently introduced into commercial and exhibition swine in the United States. The results of our spatial analysis underscore the key role of rapid viral dispersal in spreading multiple genetic lineages throughout a multistate network of agricultural fairs, providing opportunities for divergent lineages to coinfect, reassort, and generate new viral genotypes. The higher genetic diversity of genotypes cocirculating in exhibition swine since 2013 could facilitate the evolution of new reassortants, potentially with even greater ability to cause severe infections in humans or cause human-to-human transmission, highlighting the need for continued vigilance. C1 [Nelson, Martha I.] NIH, Fogarty Int Ctr, Bldg 10, Bethesda, MD 20892 USA. [Stucker, Karla M.; Schobel, Seth A.; Das, Suman R.; Dugan, Vivien G.; Wentworth, David E.] J Craig Venter Inst, Rockville, MD USA. [Trovao, Nidia S.] Katholieke Univ Leuven, Leuven, Belgium. [Nelson, Sarah W.; Nolting, Jacqueline M.; Bowman, Andrew S.] Ohio State Univ, Columbus, OH 43210 USA. [Sreevatsan, Srinand] Univ Minnesota, St Paul, MN 55108 USA. [Killian, Mary L.] USDA, Natl Vet Serv Labs, Ames, IA 50010 USA. [Stucker, Karla M.] George Sch, Newtown, PA USA. [Schobel, Seth A.] Uniformed Serv Univ Hlth Sci, Bethesda, MD USA. [Das, Suman R.] Vanderbilt Univ, Med Ctr, Dept Med, Ctr Translat Immunol & Infect Dis, Nashville, TN USA. [Dugan, Vivien G.] NIAID, Off Genom & Adv Technol, NIH, Rockville, MD USA. [Wentworth, David E.] Ctr Dis Control & Prevent, Virol Surveillance & Diag Branch, Influenza Div, Atlanta, GA USA. RP Bowman, AS (reprint author), Ohio State Univ, Columbus, OH 43210 USA. EM bowman.214@osu.edu OI Sreevatsan, Srinand/0000-0002-5162-2403; Sequeira Trovao, Nidia/0000-0002-2106-1166 FU HHS \ National Institutes of Health (NIH) [HHSN272201400006C, HHSN272201400008C, HHSN266200700007C, HHSN272200900007C, U19-AI-110819] FX This work, including the efforts of Sarah W. Nelson, Jacqueline M. Nolting, and Andrew S. Bowman, was funded by HHS vertical bar National Institutes of Health (NIH) (HHSN272201400006C). This work, including the efforts of Martha I. Nelson, was funded by HHS vertical bar National Institutes of Health (NIH) (HHSN272201400008C). This work, including the efforts of Sarah W. Nelson, Srinand Sreevatsan, Jacqueline M. Nolting, and Andrew S. Bowman, was funded by HHS vertical bar National Institutes of Health (NIH) (HHSN266200700007C). This work, including the efforts of Karla M. Stucker, Seth A. Schobel, Suman R. Das, Vivien G. Dugan, and David E. Wentworth, was funded by HHS vertical bar National Institutes of Health (NIH) (HHSN272200900007C). This work, including the efforts of Suman R. Das and David E. Wentworth, was funded by HHS vertical bar National Institutes of Health (NIH) (U19-AI-110819). NR 17 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD DEC PY 2016 VL 90 IS 23 BP 10963 EP 10971 DI 10.1128/JVI.01457-16 PG 9 WC Virology SC Virology GA EE8VL UT WOS:000389904500044 PM 27681134 ER PT J AU Liu, B Asseng, S Muller, C Ewert, F Elliott, J Lobell, DB Martre, P Ruane, AC Wallach, D Jones, J Rosenzweig, C Aggarwal, PK Alderman, PD Anothai, J Basso, B Biernath, C Cammarano, D Challinor, A Deryng, D De Sanctis, G Doltra, J Fereres, E Folberth, C Garcia-Vila, M Gayler, S Hoogenboom, G Hunt, LA Izaurralde, RC Jabloun, M Jones, CD Kersebaum, KC Kimball, BA Koehler, AK Kumar, SN Nendel, C O'Leary, GJ Olesen, JE Ottman, MJ Palosuo, T Prasad, PVV Priesack, E Pugh, TAM Reynolds, M Rezaei, EE Rotter, RP Schmid, E Semenov, MA Shcherbak, I Stehfest, E Stockle, CO Stratonovitch, P Streck, T Supit, I Tao, F Thorburn, P Waha, K Wall, GW Wang, E White, JW Wolf, J Zhao, Z Zhu, Y AF Liu, Bing Asseng, Senthold Muller, Christoph Ewert, Frank Elliott, Joshua Lobell, David B. Martre, Pierre Ruane, Alex C. Wallach, Daniel Jones, JamesW. Rosenzweig, Cynthia Aggarwal, Pramod K. Alderman, Phillip D. Anothai, Jakarat Basso, Bruno Biernath, Christian Cammarano, Davide Challinor, Andy Deryng, Delphine De Sanctis, Giacomo Doltra, Jordi Fereres, Elias Folberth, Christian Garcia-Vila, Margarita Gayler, Sebastian Hoogenboom, Gerrit Hunt, Leslie A. Izaurralde, Roberto C. Jabloun, Mohamed Jones, Curtis D. Kersebaum, Kurt C. Kimball, Bruce A. Koehler, Ann-Kristin Kumar, Soora Naresh Nendel, Claas O'Leary, Garry J. Olesen, Jorgen E. Ottman, Michael J. Palosuo, Taru Prasad, P. V. Vara Priesack, Eckart Pugh, Thomas A. M. Reynolds, Matthew Rezaei, Ehsan E. Rotter, Reimund P. Schmid, Erwin Semenov, Mikhail A. Shcherbak, Iurii Stehfest, Elke Stockle, Claudio O. Stratonovitch, Pierre Streck, Thilo Supit, Iwan Tao, Fulu Thorburn, Peter Waha, Katharina Wall, Gerard W. Wang, Enli White, Jeffrey W. Wolf, Joost Zhao, Zhigan Zhu, Yan TI Similar estimates of temperature impacts on global wheat yield by three independent methods SO NATURE CLIMATE CHANGE LA English DT Article ID CLIMATE-CHANGE; WINTER-WHEAT; CROP YIELDS; HEAT; CO2; TRENDS; METAANALYSIS; VARIABILITY; SYSTEMS; GROWTH AB The potential impact of global temperature change on global crop yield has recently been assessed with different methods. Here we show that grid-based and point-based simulations and statistical regressions (from historic records), without deliberate adaptation or CO2 fertilization effects, produce similar estimates of temperature impact on wheat yields at global and national scales. With a 1 degrees C global temperature increase, global wheat yield is projected to decline between 4.1% and 6.4%. Projected relative temperature impacts from different methods were similar for major wheat-producing countries China, India, USA and France, but less so for Russia. Point-based and grid-based simulations, and to some extent the statistical regressions, were consistent in projecting that warmer regions are likely to suffer more yield loss with increasing temperature than cooler regions. By forming a multi-method ensemble, it was possible to quantify 'method uncertainty' in addition to model uncertainty. This significantly improves confidence in estimates of climate impacts on global food security. C1 [Liu, Bing; Zhu, Yan] Nanjing Agr Univ, Jiangsu Collaborat Innovat Ctr Modern Crop Prod, Jiangsu Key Lab Informat Agr, Natl Engn & Technol Ctr Informat Agr, Nanjing 210095, Jiangsu, Peoples R China. [Liu, Bing; Asseng, Senthold; Jones, JamesW.; Hoogenboom, Gerrit] Univ Florida, Agr & Biol Engn Dept, Gainesville, FL 32611 USA. [Muller, Christoph; Waha, Katharina] Potsdam Inst Climate Impact Res, D-14473 Potsdam, Germany. [Ewert, Frank; Rezaei, Ehsan E.] Univ Bonn, Inst Crop Sci & Resource Conservat INRES, D-53115 Bonn, Germany. [Ewert, Frank] Leibniz Ctr Agr Landscape Res ZALF, D-15374 Muncheberg, Germany. [Elliott, Joshua; Ruane, Alex C.; Rosenzweig, Cynthia; Deryng, Delphine] Columbia Univ, Ctr Climate Syst Res, New York, NY 10025 USA. [Elliott, Joshua; Deryng, Delphine] Univ Chicago, Computat Inst, Chicago, IL 60637 USA. [Lobell, David B.] Stanford Univ, Dept Environm Earth Syst Sci, Stanford, CA 94305 USA. [Lobell, David B.] Stanford Univ, Ctr Food Secur & Environm, Stanford, CA 94305 USA. [Martre, Pierre] Montpellier SupAgro, INRA, UMR LEPSE, F-34060 Montpellier, France. [Ruane, Alex C.; Rosenzweig, Cynthia] NASA, Goddard Inst Space Studies, New York, NY 10025 USA. [Wallach, Daniel] INRA, Agrosyst & Dev Terr AGIR UMR1248, F-31326 Castanet Tolosan, France. [Aggarwal, Pramod K.] CIMMYT, Borlaug Inst South Asia, CGIAR Res Program Climate Change Agr & Food Secur, New Delhi 110012, India. [Alderman, Phillip D.] Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74078 USA. [Anothai, Jakarat] Prince Songkla Univ, Fac Nat Resources, Dept Plant Sci, Hat Yai 90112, Thailand. [Basso, Bruno; Shcherbak, Iurii] Michigan State Univ, Dept Geol Sci, E Lansing, MI 48823 USA. [Basso, Bruno; Shcherbak, Iurii] Michigan State Univ, WK Kellogg Biol Stn, E Lansing, MI 48823 USA. [Biernath, Christian; Priesack, Eckart] German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Ecol, D-85764 Neuherberg, Germany. [Cammarano, Davide] James Hutton Inst Invergowrie, Dundee DD2 5DA, Scotland. [Challinor, Andy; Koehler, Ann-Kristin] Univ Leeds, Sch Earth & Environm, Inst Climate & Atmospher Sci, Leeds LS2 9JT, W Yorkshire, England. [Challinor, Andy] Int Ctr Trop Agr CIAT, CGIAR ESSP Program Climate Change Agr & Hod Secur, Cali, Colombia. [De Sanctis, Giacomo] European Commiss, Joint Res Ctr, Via Enrico Fermi 2749, I-21027 Ispra, Italy. [Doltra, Jordi] Cantabrian Agr Res & Training Ctr CIFA, Muriedas 39600, Spain. [Fereres, Elias; Garcia-Vila, Margarita] Univ Cordoba, Dept Agron, Apartado 3048, E-14080 Cordoba, Spain. [Folberth, Christian] Univ Munich, Dept Geog, D-80333 Munich, Germany. Int Inst Appl Syst Anal, Ecosyst Serv & Management Program, Schlosspl 1, A-2361 Laxenburg, Austria. [Gayler, Sebastian; Streck, Thilo] Univ Hohenheim, Inst Soil Sci & Land Evaluat, D-70599 Stuttgart, Germany. [Hoogenboom, Gerrit] Washington State Univ, AgWeatherNet Program, Prosser, WA 99350 USA. [Hunt, Leslie A.] Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada. [Izaurralde, Roberto C.; Jones, Curtis D.] Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. [Izaurralde, Roberto C.] Texas A&M Univ, Texas A&M AgriLife Res & Extens Ctr, Temple, TX 76502 USA. [Jabloun, Mohamed; Olesen, Jorgen E.] Aarhus Univ, Dept Agroecol, DK-8830 Tjele, Denmark. [Kersebaum, Kurt C.; Nendel, Claas] Leibniz Ctr Agr Landscape Res, Inst Landscape Syst Anal, D-15374 Muncheberg, Germany. [Kimball, Bruce A.; Wall, Gerard W.; White, Jeffrey W.] ARS, USDA, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Kumar, Soora Naresh] IARI PUSA, Indian Agr Res Inst, Ctr Environm Sci & Climate Resilient Agr, New Delhi 110012, India. [O'Leary, Garry J.] Dept Econ Dev Jobs Transport & Resources, Landscape & Water Sci, Horsham, Vic 3400, Australia. [Ottman, Michael J.] Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA. [Palosuo, Taru; Rotter, Reimund P.; Tao, Fulu] Nat Resources Inst Finland Luke, FI-00790 Helsinki, Finland. [Prasad, P. V. Vara] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Pugh, Thomas A. M.] Karlsruhe Inst Technol, Inst Meteorol & Climate Res Atmospher Environm Re, D-82467 Garmisch Partenkirchen, Germany. [Pugh, Thomas A. M.] Univ Birmingham, Sch Geog Earth & Environm Sci, Birmingham B15 2TT, W Midlands, England. [Pugh, Thomas A. M.] Univ Birmingham, Birmingham Inst Forest Res, Birmingham B15 2TT, W Midlands, England. [Reynolds, Matthew] CIMMYT Int, AP 6-641, Mexico City 06600, DF, Mexico. [Rezaei, Ehsan E.] Ctr Dev Res ZEF, Walter Flex Str 3, D-53113 Bonn, Germany. [Rotter, Reimund P.] Georg August Univ Gottingen, Grisebachstr 6, D-37077 Gottingen, Germany. [Schmid, Erwin] Univ Nat Resources & Life Sci, A-1180 Vienna, Austria. [Semenov, Mikhail A.; Stratonovitch, Pierre] Rothamsted Res, Computat & Syst Biol Dept, Harpenden AL5 2JQ, Herts, England. [Stehfest, Elke] PBL Netherlands Environm Assessment Agcy, NL-3720 AH Bilthoven, Netherlands. [Stockle, Claudio O.] Washington State Univ, Dept Biol Syst Engn, Pullman, WA 99164 USA. [Supit, Iwan; Wolf, Joost] Wageningen Univ & Res, PPS, WSG, NL-6700 AA Wageningen, Netherlands. [Supit, Iwan; Wolf, Joost] Wageningen Univ & Res, CALM, NL-6700 AA Wageningen, Netherlands. [Tao, Fulu] Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China. [Thorburn, Peter; Waha, Katharina] CSIRO Agr & Food, St Lucia, Qld 4067, Australia. [Wang, Enli; Zhao, Zhigan] CSIRO Agr & Food, Black Mt, ACT 2601, Australia. [Zhao, Zhigan] China Agr Univ, Dept Agron & Biotechnol, Yuanmingyuan West Rd 2, Beijing 100193, Peoples R China. RP Zhu, Y (reprint author), Nanjing Agr Univ, Jiangsu Collaborat Innovat Ctr Modern Crop Prod, Jiangsu Key Lab Informat Agr, Natl Engn & Technol Ctr Informat Agr, Nanjing 210095, Jiangsu, Peoples R China. EM yanzhu@njau.edu.cn RI Palosuo, Taru/B-9593-2012; Doltra, Jordi/C-2106-2015; Challinor, Andrew/C-4992-2008; OI Palosuo, Taru/0000-0003-4322-3450; Challinor, Andrew/0000-0002-8551-6617; Martre, Pierre/0000-0002-7419-6558; Wallach, Daniel/0000-0003-3500-8179; Schmid, Erwin/0000-0003-4783-9666; Muller, Christoph/0000-0002-9491-3550 FU National High-Tech Research and Development Program of China [2013AA100404]; National Natural Science Foundation of China [31271616, 31611130182, 41571088, 31561143003]; National Research Foundation for the Doctoral Program of Higher Education of China [20120097110042]; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD); China Scholarship Council; IFPRI through the Global Futures and Strategic Foresight project; CGIAR Research Program on Climate Change, Agriculture and Food Security (CCAFS); CGIAR Research Program on Wheat; Agricultural Model Intercomparison and Improvement Project (AgMIP) FX This work was supported by the National High-Tech Research and Development Program of China (2013AA100404), the National Natural Science Foundation of China (31271616, 31611130182, 41571088 and 31561143003), the National Research Foundation for the Doctoral Program of Higher Education of China (20120097110042), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and the China Scholarship Council. We would like to acknowledge support provided by IFPRI through the Global Futures and Strategic Foresight project, the CGIAR Research Program on Climate Change, Agriculture and Food Security (CCAFS), the CGIAR Research Program on Wheat and the Agricultural Model Intercomparison and Improvement Project (AgMIP). NR 50 TC 1 Z9 1 U1 48 U2 48 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1758-678X EI 1758-6798 J9 NAT CLIM CHANGE JI Nat. Clim. Chang. PD DEC PY 2016 VL 6 IS 12 BP 1130 EP + DI 10.1038/NCLIMATE3115 PG 8 WC Environmental Sciences; Environmental Studies; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA EE2RP UT WOS:000389432200023 ER PT J AU Gorski, L Parker, CT Liang, AS Walker, S Romanolo, KF AF Gorski, Lisa Parker, Craig T. Liang, Anita S. Walker, Samarpita Romanolo, Kelly F. TI The Majority of Genotypes of the Virulence Gene inlA Are Intact among Natural Watershed Isolates of Listeria monocytogenes from the Central California Coast SO PLOS ONE LA English DT Article ID PREMATURE STOP CODON; TO-EAT FOODS; PHENOTYPIC CHARACTERIZATION; MUTATIONS; EVOLUTION; STRAINS; ENVIRONMENTS; INTERNALIN; IDENTIFICATION; PREVALENCE AB Internalin A is an essential virulence gene involved in the uptake of the foodborne pathogen Listeria monocytogenes into host cells. It is intact in clinical strains and often truncated due to Premature Stop Codons (PMSCs) in isolates from processed foods and processing facilities. Less information is known about environmental isolates. We sequenced the inlA alleles and did Multi Locus Variable Number Tandem Repeat Analysis (MLVA) on 112 L. monocytogenes isolates from a 3-year period from naturally contaminated watersheds near a leafy green growing area in Central California. The collection contained 14 serotype 1/2a, 12 serotype 1/2b, and 86 serotype 4b strains. Twenty-seven different inlA alleles were found. Twenty-three of the alleles are predicted to encode intact copies of InlA, while three contain PMSCs. Another allele has a 9-nucleotide deletion, previously described for a clinical strain, indicating that it is still functional. Intact inlA genes were found in 101 isolates, and 8 isolates contained the allele predicted to contain the 3-amino acid deletion. Both allele types were found throughout the 3-year sampling period. Three strains contained inlA alleles with PMSCs, and these were found only during the first 3 months of the study. SNP analysis of the intact alleles indicated clustering of alleles based on serotype and lineage with serotypes 1/2b and 4b (lineage I strains) clustering together, and serotype 1/2a (lineage II strains) clustering separately. The combination of serotype, MLVA types, and inlA allele types indicate that the 112 isolates reflect at least 49 different strains of L. monocytogenes. The finding that 90% of environmental L. monocytogenes isolates contain intact inlA alleles varies significantly from isolates found in processing plants. This information is important to public health labs and growers as to the varieties of L. monocytogenes that could potentially contaminate fresh produce in the field by various means. C1 [Gorski, Lisa; Parker, Craig T.; Liang, Anita S.; Walker, Samarpita; Romanolo, Kelly F.] ARS, Prod Safety & Microbiol Res Unit, USDA, Albany, CA 94710 USA. [Romanolo, Kelly F.] USDA, Food Safety Inspect Serv, Alameda, CA USA. RP Gorski, L (reprint author), ARS, Prod Safety & Microbiol Res Unit, USDA, Albany, CA 94710 USA. EM lisa.gorski@ars.usda.gov FU Agricultural Research Service CRIS project [5325-42000-050-00D]; Food and Drug Administration [224-11-2044]; FDA [224-11-2044] FX This work was funded through Agricultural Research Service CRIS project 5325-42000-050-00D and Food and Drug Administration grant #224-11-2044. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; The authors thank M. Cooley for information and advice. This work was funded through Agricultural Research Service CRIS project 5325-42000-050-00D and FDA grant #224-11-2044. NR 42 TC 0 Z9 0 U1 2 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 1 PY 2016 VL 11 IS 12 AR e0167566 DI 10.1371/journal.pone.0167566 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EE3JC UT WOS:000389482700196 PM 27907153 ER PT J AU Smykal, P Varshney, RK Singh, VK Coyne, CJ Domoney, C Kejnovsky, E Warkentin, T AF Smykal, Petr Varshney, Rajeev K. Singh, Vikas K. Coyne, Clarice J. Domoney, Claire Kejnovsky, Eduard Warkentin, Thomas TI From Mendel's discovery on pea to today's plant genetics and breeding SO THEORETICAL AND APPLIED GENETICS LA English DT Review ID MARKER-ASSISTED SELECTION; FRAGMENT-LENGTH-POLYMORPHISMS; BACTERIAL-BLIGHT RESISTANCE; RED SPRING WHEAT; QUANTITATIVE-TRAIT LOCI; TOLERANT RICE CULTIVARS; ZINC-FINGER NUCLEASES; LEAF RUST RESISTANCE; TRANSPOSABLE ELEMENTS; GENOME EVOLUTION AB This work discusses several selected topics of plant genetics and breeding in relation to the 150th anniversary of the seminal work of Gregor Johann Mendel. In 2015, we celebrated the 150th anniversary of the presentation of the seminal work of Gregor Johann Mendel. While Darwin's theory of evolution was based on differential survival and differential reproductive success, Mendel's theory of heredity relies on equality and stability throughout all stages of the life cycle. Darwin's concepts were continuous variation and "soft" heredity; Mendel espoused discontinuous variation and "hard" heredity. Thus, the combination of Mendelian genetics with Darwin's theory of natural selection was the process that resulted in the modern synthesis of evolutionary biology. Although biology, genetics, and genomics have been revolutionized in recent years, modern genetics will forever rely on simple principles founded on pea breeding using seven single gene characters. Purposeful use of mutants to study gene function is one of the essential tools of modern genetics. Today, over 100 plant species genomes have been sequenced. Mapping populations and their use in segregation of molecular markers and marker-trait association to map and isolate genes, were developed on the basis of Mendel's work. Genome-wide or genomic selection is a recent approach for the development of improved breeding lines. The analysis of complex traits has been enhanced by high-throughput phenotyping and developments in statistical and modeling methods for the analysis of phenotypic data. Introgression of novel alleles from landraces and wild relatives widens genetic diversity and improves traits; transgenic methodologies allow for the introduction of novel genes from diverse sources, and gene editing approaches offer possibilities to manipulate gene in a precise manner. C1 [Smykal, Petr] Palacky Univ Olomouc, Dept Bot, Fac Sci, Slechtitelu 27, Olomouc, Czech Republic. [Varshney, Rajeev K.; Singh, Vikas K.] Int Crops Res Inst Semi Arid Trop, Hyderabad, Andhra Pradesh, India. [Coyne, Clarice J.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. [Domoney, Claire] Norwich Res Pk, John Innes Ctr, Norwich, Norfolk, England. [Kejnovsky, Eduard] Acad Sci Czech Republic, Inst Biophys, Dept Plant Dev Genet, Brno, Czech Republic. [Warkentin, Thomas] Univ Saskatchewan, Ctr Crop Dev, Saskatoon, SK, Canada. RP Smykal, P (reprint author), Palacky Univ Olomouc, Dept Bot, Fac Sci, Slechtitelu 27, Olomouc, Czech Republic. EM petr.smykal@upol.cz OI Smykal, Petr/0000-0002-6117-8510 FU Czech Science Foundation [15-02891S, 14-11782S]; Palacky University [IGA 2015_1, IGA 2016_1] FX This research was supported by the Czech Science Foundation (Grant 15-02891S to E.K. and 14-11782S to P.S.) and Palacky University Grant IGA 2015_1 and IGA 2016_1 to P.S. Various colleagues are acknowledged for their fruitful discussions on the earlier versions of the manuscript. NR 220 TC 1 Z9 1 U1 46 U2 46 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD DEC PY 2016 VL 129 IS 12 BP 2267 EP 2280 DI 10.1007/s00122-016-2803-2 PG 14 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA EE0HJ UT WOS:000389254700004 PM 27717955 ER PT J AU Chen, JL Guttieri, MJ Zhang, JL Hole, D Souza, E Goates, B AF Chen, Jianli Guttieri, Mary J. Zhang, Junli Hole, David Souza, Edward Goates, Blair TI A novel QTL associated with dwarf bunt resistance in Idaho 444 winter wheat SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID COMMON BUNT; LINKAGE MAPS; REGISTRATION; IDENTIFICATION; GENE; LANDRACES; RACES; LINES AB A novel QTL, Q.DB.ui-7DS, and the PCR-based markers identified in the current study will accelerate variety development for resistance to dwarf and common bunt of wheat. Dwarf bunt [Tilletia controversa J.G. Kuhn [as 'contraversa'], in Rabenhorst, Hedwigia 13: 188 (1874)] is a destructive disease of wheat (Triticum aestivum L.) that reduces grain yield and quality. A number of distinct genes conferring resistance to dwarf bunt have been used by breeding programs for nearly 100 years. However, few markers were identified that can be used in selection of dwarf bunt resistance. A recombinant inbred line (RIL) population derived from the bunt-resistant germplasm, Idaho 444 (IDO444), and the susceptible cultivar, Rio Blanco, was evaluated for phenotypic reaction to dwarf bunt inoculation in four trials in two locations (USU and USDA) over 3 years. The population was genotyped with the Diversity Arrays Technology (DArT) and the Illumina Infinium 9K iSelect marker platforms. A total of three QTL were detected, and resistant alleles were from IDO444. QTL Q.DB.ui-7DS on 7DS was determined based on the location of a DArT marker wPt-2565 (X116197), which was consistently detected and explained 32 to 56 % of phenotypic variation among the four trials. QTL Q.DB.ui-1A on 1A was detected in three Utah State University (USU) trials and explained 11-15 % of phenotypic variation. QTL Q.DB.ui-2B on 2B was detected in two USU and one United States Department of Agriculture (USDA) trials and explained up to 6 % of phenotypic variation. Two PCR-based markers were developed based on the sequence of wPt-2565 and validated in the RIL population and used in genotyping of dwarf bunt differential lines, known resistance sources, and resistant cultivars. C1 [Chen, Jianli] Univ Idaho, 1693 S 2700 W, Aberdeen, ID 83210 USA. [Guttieri, Mary J.] ARS, USDA, Ctr Grain & Anim Hlth Res, Hard Winter Wheat Genet Res Unit, 4011 Throckmorton Hall, Manhattan, KS 66506 USA. [Zhang, Junli] Univ Calif Davis, Dept Plant Sci, One Shields Ave, Davis, CA 95616 USA. [Hole, David] Utah State Univ, 2325 Old Main Hill, Logan, UT 84322 USA. [Souza, Edward] Bayer Crop Sci, Beaver Crossing, NE 68313 USA. [Goates, Blair] USDA ARS, Aberdeen, ID 83210 USA. RP Hole, D (reprint author), Utah State Univ, 2325 Old Main Hill, Logan, UT 84322 USA. EM david.hole@gmail.com OI Hole, David/0000-0003-1325-3181 FU National Research Initiative Competitive Grants CAP project from the USDA NIFA [2011-68002-30029]; Idaho Agricultural Experiment Station [IDA01483]; Idaho Wheat Commission [BJKW32]; Utah Agricultural Experiment Station (UAES) [UTA1215] FX Authors acknowledge Dr. Andrzej Kilian at Diversity Array Technologies for providing sequence information for specific DArT markers, thanks Dr. Rui Wang and Mr. Weidong Zhao for technical assistance. This project was supported by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA NIFA; by the Idaho Agricultural Experiment Station (project IDA01483); by the Idaho Wheat Commission (project BJKW32); and by the Utah Agricultural Experiment Station (UAES project UTA1215). Approved as UAES journal paper number 8897. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US. Department of Agriculture. The US. Department of Agriculture (USDA) prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable, sex, marital status, familial status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal, or because all or part of an individual's income is derived from any public assistance program. (Not all prohibited bases apply to all programs.) Persons with disabilities who require alternative means for communication of program information (Braille, large print, audiotape, etc.) should contact USDA's TARGET Center at (202) 720-2600 (voice and TDD). To file a complaint of discrimination, write to USDA, Director, Office of Civil Rights, 1400 Independence Avenue, S.W., Washington, D.C. 20250-9410, or call (800) 795-3272 (voice) or (202) 720-6382 (TDD). USDA is an equal opportunity provider and employer. NR 35 TC 1 Z9 1 U1 6 U2 6 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD DEC PY 2016 VL 129 IS 12 BP 2313 EP 2322 DI 10.1007/s00122-016-2783-2 PG 10 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA EE0HJ UT WOS:000389254700007 PM 27681089 ER PT J AU Lietzow, CD Zhu, HY Pandey, S Havey, MJ Weng, YQ AF Lietzow, Calvin D. Zhu, Huayu Pandey, Sudhakar Havey, Michael J. Weng, Yiqun TI QTL mapping of parthenocarpic fruit set in North American processing cucumber SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID RECOMBINANT INBRED LINES; QUANTITATIVE TRAIT LOCI; SATIVUS L; HORMONAL-REGULATION; CASSIA-FASCICULATA; AUGMENTED DESIGNS; PICKLING CUCUMBER; AUXIN TRANSPORT; ANNUAL LEGUME; TOMATO AB Through a novel phenotyping method, four QTLs were consistently associated with increased parthenocarpic fruit set in North American processing cucumber that accounted for over 75 % of observed phenotypic variation. Parthenocarpy is a desirable trait with potential for increasing yield and quality in processing cucumber production. Although many successful parthenocarpic fresh market cucumber varieties have been developed, the genetic and molecular mechanisms behind parthenocarpic expression in cucumber remain largely unknown. Since parthenocarpy is an important yield component, it is difficult to separate the true parthenocarpic character from other yield related traits. In the present study, we developed a novel phenotypic approach for parthenocarpic fruit set focusing on early fruit development. Two hundred and five F-3 families derived from a cross between the highly parthenocarpic line 2A and low parthenocarpic line Gy8 were phenotypically evaluated in three greenhouse experiments. Seven QTLs associated with parthenocarpic fruit set were detected. Among them, one each on chromosomes 5 and 7 (parth5.1 and parth7.1) and two on chromosome 6 (parth6.1 and parth6.2) were consistently identified in all experiments, but their relative contribution to the total phenotypic variation was dependent on plant growth stages. While each of the four QTLs had almost equal contribution to the expression of the trait at commercial harvest stage, parth7.1 played an important role in early parthenocarpic fruit set. The results suggested that parthenocarpic fruit set can be accurately evaluated with as few as 20 nodes of growth. The QTLs identified in this study for parthenocarpic fruit set are a valuable resource for cucumber breeders interested in developing parthenocarpic cultivars and to researchers interested in the genetic and molecular mechanisms of parthenocarpic fruit set. C1 [Lietzow, Calvin D.; Zhu, Huayu; Pandey, Sudhakar; Havey, Michael J.; Weng, Yiqun] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Pandey, Sudhakar] Indian Inst Vegetable Res, Varanasi 221305, Uttar Pradesh, India. [Havey, Michael J.; Weng, Yiqun] Univ Wisconsin, Dept Hort, USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. RP Weng, YQ (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA.; Weng, YQ (reprint author), Univ Wisconsin, Dept Hort, USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. EM cdlietzow@gmail.com; jienaming@163.com; sudhakariivr@gmail.com; michael.havey@ars.usda.gov; yiqun.weng@wisc.edu FU US Department of Agriculture Specialty Crop Research Initiative Grant [2011-51181-30661] FX The authors thank Linda Crubaugh, Luming Yang, Changlong Wen, Bree C. Lietzow, Nick Wedde, and Mary Ann Hoffelt for their technical assistance. The authors also thank Dr. Richard Lower for providing seeds of the two parental lines and partial financial support for this project. This research was supported by a US Department of Agriculture Specialty Crop Research Initiative Grant (Project number 2011-51181-30661) to Y.W. Names are necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval of the product to the exclusion of others that may also be suitable. USDA is an equal opportunity provider and employer. NR 76 TC 0 Z9 0 U1 10 U2 10 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD DEC PY 2016 VL 129 IS 12 BP 2387 EP 2401 DI 10.1007/s00122-016-2778-z PG 15 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA EE0HJ UT WOS:000389254700014 PM 27581542 ER PT J AU Stegelmeier, BL Colegate, SM Brown, AW AF Stegelmeier, Bryan L. Colegate, Steven M. Brown, Ammon W. TI Dehydropyrrolizidine Alkaloid Toxicity, Cytotoxicity, and Carcinogenicity SO TOXINS LA English DT Review DE dehydropyrrolizidine; DHPA; pyrrolizidine; alkaloids; PA; toxic plant; pyrrolizidine alkaloid-induced cytotoxicity; toxic hepatopathy; carcinogenesis ID PYRROLIZIDINE ALKALOIDS; DNA-ADDUCTS; VENOOCCLUSIVE DISEASE; IN-VITRO; N-OXIDES; S-PHASE; RIDDELLIINE; RATS; METABOLITES; PLANTS AB Dehydropyrrolizidine alkaloid (DHPA)-producing plants have a worldwide distribution amongst flowering plants and commonly cause poisoning of livestock, wildlife, and humans. Previous work has produced considerable understanding of DHPA metabolism, toxicity, species susceptibility, conditions, and routes of exposure, and pathogenesis of acute poisoning. Intoxication is generally caused by contaminated grains, feed, flour, and breads that result in acute, high-dose, short-duration poisoning. Acute poisoning produces hepatic necrosis that is usually confirmed histologically, epidemiologically, and chemically. Less is known about chronic poisoning that may result when plant populations are sporadic, used as tisanes or herbal preparations, or when DHPAs contaminate milk, honey, pollen, or other animal-derived products. Such subclinical exposures may contribute to the development of chronic disease in humans or may be cumulative and probably slowly progress until liver failure. Recent work using rodent models suggest increased neoplastic incidence even with very low DHPA doses of short durations. These concerns have moved some governments to prohibit or limit human exposure to DHPAs. The purpose of this review is to summarize some recent DHPA research, including in vitro and in vivo DHPA toxicity and carcinogenicity reports, and the implications of these findings with respect to diagnosis and prognosis for human and animal health. C1 [Stegelmeier, Bryan L.; Colegate, Steven M.] ARS, USDA, Poisonous Plant Res Lab, 1150 East 1400 North, Logan, UT 84341 USA. [Colegate, Steven M.] Utah State Univ, Dept Anim Dairy & Vet Sci, Logan, UT 84322 USA. [Brown, Ammon W.] US Army, Inst Surg Res, Ft Sam Houston, TX 78234 USA. RP Stegelmeier, BL (reprint author), ARS, USDA, Poisonous Plant Res Lab, 1150 East 1400 North, Logan, UT 84341 USA. EM bryan.stegelmeier@ars.usda.gov; steven.colegate@usu.edu; brown.ammon@gmail.com NR 60 TC 0 Z9 0 U1 7 U2 7 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2072-6651 J9 TOXINS JI Toxins PD DEC PY 2016 VL 8 IS 12 DI 10.3390/toxins8120356 PG 15 WC Toxicology SC Toxicology GA EE1LC UT WOS:000389342000010 ER PT J AU Nowak, DJ AF Nowak, David J. TI Urban metabolism in Syracuse, NY - introduction SO URBAN ECOSYSTEMS LA English DT Editorial Material C1 [Nowak, David J.] US Forest Serv, USDA, Syracuse, NY 13210 USA. RP Nowak, DJ (reprint author), US Forest Serv, USDA, Syracuse, NY 13210 USA. EM dnowak@fs.fed.us NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1083-8155 EI 1573-1642 J9 URBAN ECOSYST JI Urban Ecosyst. PD DEC PY 2016 VL 19 IS 4 BP 1419 EP 1420 DI 10.1007/s11252-016-0615-4 PG 2 WC Biodiversity Conservation; Ecology; Environmental Sciences; Urban Studies SC Biodiversity & Conservation; Environmental Sciences & Ecology; Urban Studies GA EF0ML UT WOS:000390019400001 ER PT J AU Nowak, DJ Hoehn, RE Bodine, AR Greenfield, EJ O'Neil-Dunne, J AF Nowak, David J. Hoehn, Robert E. Bodine, Allison R. Greenfield, Eric J. O'Neil-Dunne, Jarlath TI Urban forest structure, ecosystem services and change in Syracuse, NY SO URBAN ECOSYSTEMS LA English DT Article DE Urban forest sampling; i-Tree; Urban forest monitoring; Invasive plants; Invasive pests ID CARBON STORAGE; UNITED-STATES; US CITIES; TREES; SEQUESTRATION AB The tree population within the City of Syracuse was assessed using a random sampling of plots in 1999, 2001 and 2009 to determine how the population and the ecosystem services these trees provide have changed over time. Ecosystem services and values for carbon sequestration, air pollution removal and changes in building energy use were derived using the i-Tree Eco model. In addition, photo interpretation of aerial images was used to determine changes in tree cover between the mid-1990s and 2009. Between the mid-1990s and 2003, tree cover in Syracuse exhibited a decline from 27.5 to 25.9 %, but subsequently increased to 26.9 % by 2009. The total tree population exhibited a similar pattern, dropping from 881,000 trees in 1999 to 862,000 in 2001, and then increasing to 1,087,000 trees in 2009. Most of this increase in the urban tree population is due to invasive or pioneer trees species, particularly Rhamnus cathartica, which has more than tripled in population between 2001 and 2009. Insects such as gypsy moth and emerald ash borer pose a substantial risk to altering future urban forest composition. The annual ecosystem services provided by the urban forest in relation to carbon sequestration, air pollution removal and reduction in building energy use are estimated at about $2.4 million per year. An improved understanding of urban forests and how they are changing can facilitate better management plans to sustain ecosystem services and desired forest structure for future generations. C1 [Nowak, David J.; Hoehn, Robert E.; Greenfield, Eric J.] SUNY Coll Environm Sci & Forestry, USDA Forest Serv, Northern Res Stn, Moon Lib 5, Syracuse, NY 13210 USA. [Bodine, Allison R.] SUNY Coll Environm Sci & Forestry, Davey Inst, Moon Lib 5, Syracuse, NY 13210 USA. [O'Neil-Dunne, Jarlath] Univ Vermont, Rubenstein Sch, Aiken Ctr, Spatial Anal Lab, 81 Carrigan Dr, Burlington, VT 05405 USA. RP Nowak, DJ (reprint author), SUNY Coll Environm Sci & Forestry, USDA Forest Serv, Northern Res Stn, Moon Lib 5, Syracuse, NY 13210 USA. EM dnowak@fs.fed.us FU National Science Foundation (NSF) through the Syracuse Urban Long-term Research Area Exploratory Award (ULTRA-Ex) [BCS-0948952] FX Funding for this project was provided, in part, by the National Science Foundation (NSF grant BCS-0948952) through the Syracuse Urban Long-term Research Area Exploratory Award (ULTRA-Ex). The use of trade, firm, or corporation names in this article is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the U.S. Department of Agriculture Forest Service of any product or service to the exclusion of others that may be suitable. We thank Jack Stevens for his valuable assistance in data analysis, John Stanovick for his statistical assistance and review, and Noel Schneeberger and Myrna Hall for their comments on a draft manuscript. NR 63 TC 2 Z9 3 U1 41 U2 41 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1083-8155 EI 1573-1642 J9 URBAN ECOSYST JI Urban Ecosyst. PD DEC PY 2016 VL 19 IS 4 BP 1455 EP 1477 DI 10.1007/s11252-013-0326-z PG 23 WC Biodiversity Conservation; Ecology; Environmental Sciences; Urban Studies SC Biodiversity & Conservation; Environmental Sciences & Ecology; Urban Studies GA EF0ML UT WOS:000390019400004 ER PT J AU Griffiths, NA Sebestyen, SD AF Griffiths, Natalie A. Sebestyen, Stephen D. TI Dynamic Vertical Profiles of Peat Porewater Chemistry in a Northern Peatland SO WETLANDS LA English DT Article DE Black spruce-Sphagnum ombrotrophic bog; Solute chemistry; Spatial and temporal variability; Depth profiles; Groundwater ID DISSOLVED ORGANIC-CARBON; SURFACE-WATER CHEMISTRY; PORE-WATER; SEASONAL-VARIATION; CLIMATIC-CHANGE; BLANKET BOG; MINNESOTA; GROUNDWATER; GRADIENTS; CANADA AB We measured pH, cations, nutrients, and total organic carbon (TOC) over 3 years to examine weekly to monthly variability in porewater chemistry depth profiles (0-3.0 m) in an ombrotrophic bog in Minnesota, USA. We also compared temporal variation at one location to spatial variation in depth profiles at 16 locations across the bog. Most solutes exhibited large gradients with depth. pH increased by two units and calcium concentrations increased over 20 fold with depth, and may reflect peatland development from minerotrophic to ombrotrophic conditions. Ammonium concentrations increased almost 20 fold and TOC concentrations decreased by half with depth, and these patterns likely reflect mineralization of peat or decomposition of TOC. There was also considerable temporal variation in the porewater chemistry depth profiles. Ammonium, soluble reactive phosphorus, and potassium showed greater temporal variation in near-surface porewater, while pH, calcium, and TOC varied more at depth. This variation demonstrates that deep peat porewater chemistry is not static. Lastly, temporal variation in solute chemistry depth profiles was greater than spatial variation in several instances, especially in shallow porewaters. Characterizing both temporal and spatial variability is necessary to ensure representative sampling in peatlands, especially when calculating solute pools and fluxes and parameterizing process-based models. C1 [Griffiths, Natalie A.] Oak Ridge Natl Lab, Climate Change Sci Inst, Oak Ridge, TN 37831 USA. [Griffiths, Natalie A.] Oak Ridge Natl Lab, Div Environm Sci, Oak Ridge, TN 37831 USA. [Sebestyen, Stephen D.] US Forest Serv, Northern Res Stn, USDA, Grand Rapids, MN 55744 USA. RP Griffiths, NA (reprint author), Oak Ridge Natl Lab, Climate Change Sci Inst, Oak Ridge, TN 37831 USA.; Griffiths, NA (reprint author), Oak Ridge Natl Lab, Div Environm Sci, Oak Ridge, TN 37831 USA. EM griffithsna@ornl.gov RI Sebestyen, Stephen/D-1238-2013 OI Sebestyen, Stephen/0000-0002-6315-0108 FU U.S. Department of Energy's Office of Science, Biological and Environmental Research; Northern Research Station of the USDA Forest Service; Oak Ridge National Laboratory; UT-Battelle, LLC; U.S. Department of Energy [DE-AC05-00OR22725] FX We thank the late Pat Mulholland for his guidance, mentoring, and friendship. Pat's input into the early stages of this project greatly improved our experimental design and analysis. We thank K. Oleheiser, N. Aspelin, J. Larson, C. Dorrance, D. Kyllander, R. Nettles, J. Riggs, R. Peterson, B. Munson, M. Olds, M. Wiley, and L. Kastenson for technical assistance, and P. Hanson and R. Kolka for manuscript comments and for their leadership on the Spruce and Peatland Responses Under Climatic and Environmental Change (SPRUCE) project. Comments from two anonymous reviewers greatly improved an earlier version of this manuscript. This research was part of the SPRUCE project and supported by the U.S. Department of Energy's Office of Science, Biological and Environmental Research and the Northern Research Station of the USDA Forest Service. Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the U.S. Department of Energy under contract DE-AC05-00OR22725. NR 49 TC 0 Z9 0 U1 6 U2 6 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0277-5212 EI 1943-6246 J9 WETLANDS JI Wetlands PD DEC PY 2016 VL 36 IS 6 BP 1119 EP 1130 DI 10.1007/s13157-016-0829-5 PG 12 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA EE5FE UT WOS:000389630100012 ER PT J AU Buchholz, AE Katz, AR Galloway, R Stoddard, RA Goldstein, SM AF Buchholz, A. E. Katz, A. R. Galloway, R. Stoddard, R. A. Goldstein, S. M. TI Feral Swine Leptospira Seroprevalence Survey in Hawaii, USA, 2007-2009 SO ZOONOSES AND PUBLIC HEALTH LA English DT Article DE Feral swine; leptospirosis; Hawaii; survey ID WILD BOARS; SUS-SCROFA; PREVALENCE; ANTIBODIES; OUTBREAK; FLORIDA; HUMANS; PIGS AB notifiable disease in the United States until 1995 and was reinstated to the list of nationally notifiable diseases in 2014. During the time of national surveillance, Hawaii consistently led the nation in reported annual incidence rates. Leptospirosis has remained a reportable disease in Hawaii. Significant changes have been documented since the early 1970s in the predominant serogroup infecting humans in Hawaii: infections due to Icterohaemorrhagiae have declined while infections due to Australis have increased. A recent study from Hawaii demonstrated that Australis was an uncommon infecting serogroup for small mammal hosts. Swine have not been previously studied in Hawaii but are well-recognized maintenance hosts for leptospires belonging to the Australis serogroup. This study was undertaken to assess the prevalence of Leptospira antibody in feral swine in Hawaii. From January 2007 through December 2009, blood samples were collected opportunistically from feral swine. Using the microscopic agglutination test, we found antibody titres >= 1 : 100 to leptospires in 272 (33.8%) of 804 feral swine. The most frequently reacting serovars to the swine sera were Icterohaemorrhagiae (Icterohaemorrhagiae serogroup) (41.5%) and Bratislava (Australis serogroup) (33.8%). The high seroprevalence and presumptively infecting serovars suggest a link between swine and human infection. C1 [Buchholz, A. E.; Katz, A. R.] Univ Hawaii, Honolulu, HI 96822 USA. [Galloway, R.; Stoddard, R. A.] Ctr Dis Control & Prevent, Atlanta, GA USA. [Goldstein, S. M.] USDA, Honolulu, HI USA. [Buchholz, A. E.] USDA, Indianapolis, IN USA. RP Katz, AR (reprint author), Univ Hawaii, Off Publ Hlth Studies, 1960 East West Rd,Biomed Sci Bldg,Room D104M, Honolulu, HI 96822 USA. EM katz@hawaii.edu NR 21 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1863-1959 EI 1863-2378 J9 ZOONOSES PUBLIC HLTH JI Zoonoses Public Health PD DEC PY 2016 VL 63 IS 8 BP 584 EP 587 DI 10.1111/zph.12266 PG 4 WC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences SC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences GA ED8VJ UT WOS:000389148700002 PM 26969849 ER PT J AU Korczak, R Marquart, L Slavin, JL Ringling, K Chu, YF O'Shea, M Harriman, C Toups, K de Vries, J Jacques, P Klurfeld, DM Camire, ME Unnevehr, L AF Korczak, Renee Marquart, Len Slavin, Joanne L. Ringling, Keagan Chu, YiFang O'Shea, Marianne Harriman, Cynthia Toups, Kelly de Vries, Jan Jacques, Paul Klurfeld, Davi M. Camire, Mary Ellen Unnevehr, Laurian TI Thinking critically about whole-grain definitions: summary report of an interdisciplinary roundtable discussion at the 2015 Whole Grains Summit SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE definitions; interdisciplinary; whole grain; whole-grain foods standard methods; HEALTHGRALN; health benefits ID CARDIOVASCULAR-DISEASE; WEIGHT-GAIN; PROSPECTIVE COHORT; CEREAL FIBER; CONSUMPTION; RISK; RECOMMENDATIONS; METAANALYSIS; MORTALITY; PRODUCTS AB Definitions for whole grain (WG) have been published by governments, the food industry, and grain organizations and generally fail into 2 categories: WG and WG food. WG definitions focus on the principal components of the WGs and their proportions, whereas WG-food definitions describe the quantity of WGs present in food. In the United States, widespread agreement exists on the main parts of a definition for a WG, with a definition for a WG food still in its early stages; a standard definition that has been universally accepted does not exist. Furthermore, nutrition policy advises consumers to eat WGs for at least one-half of their total grain intake (2010 and 2015 Dietary Guidelines for Americans), but confusion exists over which foods are considered WGs and how much is needed to achieve health benefits. In December 2014, a workshop sponsored by the subcommittee on collaborative process of the US Government's Interagency Committee on Human Nutrition Research convened in Washington, DC, and recognized WG definitions as a key nutrition and public health related issue that could benefit from further collaboration. As a follow-up to that meeting, an interdisciplinary roundtable meeting was organized at the Whole Grains Summit on 25 June 2015 in Portland, Oregon, to help resolve the issue. This article summarizes the main opportunities and challenges that were identified during the meeting for defining WGs and WG foods internationally. Definitions of WGs and WG foods that are uniformly adopted by research, food industry, consumer, and public health communities are needed to enable comparison of research results across populations. C1 [Korczak, Renee] Premier Nutr LLC, Minneapolis, MN 55401 USA. [Marquart, Len; Slavin, Joanne L.; Ringling, Keagan] Univ Minnesota, Dept Food Sci & Nutr, 1334 Eckles Ave, St Paul, MN USA. [Chu, YiFang; O'Shea, Marianne] PepsiCo R&D Nutr, Quaker Oats Ctr Excellence, Barrington, IL USA. [Harriman, Cynthia; Toups, Kelly] Oldways Whole Grains Council, Boston, MA USA. [de Vries, Jan] De Vries Nutr Solut, Gorssel, Netherlands. [Jacques, Paul] Tufts Univ, Gerald J & Dorothy R Friedman Sch Nutr Sci & Poli, Boston, MA 02111 USA. [Jacques, Paul] Tufts Univ, USDA ARS, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Klurfeld, Davi M.] USDA ARS, Beltsville, MD USA. [Camire, Mary Ellen] Univ Maine, Sch Food & Agr, Orono, ME USA. [Unnevehr, Laurian] Univ Illinois, Dept Agr & Consumer Econ, Urbana, IL USA. RP Korczak, R (reprint author), Premier Nutr LLC, Minneapolis, MN 55401 USA. EM korczak.renee@gmail.com FU Quaker Oats Center of Excellence FX Supported by Quaker Oats Center of Excellence. This is a free access article, distributed under terms (http://www.nutrition.org/publications/guidelines-and-policies/license/) that permit unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. NR 34 TC 1 Z9 1 U1 5 U2 5 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD DEC PY 2016 VL 104 IS 6 BP 1508 EP 1514 DI 10.3945/ajcn.115.126672 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA EE0RV UT WOS:000389287500004 PM 27806974 ER PT J AU Sawaengsri, H Bergethon, PR Qiu, WQ Scott, TM Jacques, PF Selhub, J Paul, L AF Sawaengsri, Hathairat Bergethon, Peter R. Qiu, Wei Qiao Scott, Tammy M. Jacques, Paul F. Selhub, Jacob Paul, Ligi TI Transcobalamin 776C -> G polymorphism is associated with peripheral neuropathy in elderly individuals with high folate intake SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE folate; folic acid; peripheral neuropathy; TCN2 776C>G polymorphism; transcobalamin; vitamin B-12 ID FOLIC-ACID; VITAMIN-B-12 STATUS; COBALAMIN DEFICIENCY; COGNITIVE FUNCTION; TOTAL HOMOCYSTEINE; SERUM FOLATE; POPULATION; PLASMA; ANEMIA; BLOOD AB Background: The 776C -> G polymorphism of the vitamin B-12 transport protein transcobalamin gene (TCN2) (rs1801198; Pro259Arg) is associated with a lower holotranscobalamin concentration in plasma. This effect may reduce the availability of vitamin B-12 to tissues even when vitamin B-12 intake is adequate. Clinical outcomes associated with vitamin B-12 insufficiency could potentially be worsened by high folate intake. Objective: We determined the association of the TCN2 776C -> G polymorphism and folate intake with peripheral neuropathy in elders with normal plasma concentrations of vitamin B-12. Design: Participants in this cross-sectional study (n = 171) were from a cohort of community-based, home-bound elderly individuals aged >= 60y who underwent an evaluation by physicians including an assessment for peripheral neuropathy. Participants were administered food-frequency and general health status questionnaires, anthropometric measurements were taken, and a fasting blood sample from each subject was collected. Results: Odds of neuropathy were 3-fold higher for GG genotypes than for CC genotypes (OR: 3.33; 95% CI: 1.15, 9.64). When folate intake was >2 times the Recommended Dietary Allowance (800 mu g), GG genotypes had 6.9-fold higher odds of neuropathy than CC genotypes (OR: 6.9; 95% CI: 1.31, 36.36). There was no difference between the genotypes in the odds of peripheral neuropathy when folate intake was <= 800 mu g (OR: 1.5; 95% CI: 0.18, 12.33). Conclusion: The TCN2 776C -> G polymorphism is associated with increased odds of peripheral neuropathy in the elderly, even with a normal vitamin B-12 status, especially if their folate intake is >2 times the Recommended Dietary Allowance. C1 [Sawaengsri, Hathairat; Scott, Tammy M.; Jacques, Paul F.; Selhub, Jacob; Paul, Ligi] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Bergethon, Peter R.] Pfizer Inc, Neurosci & Pain Res Unit, Cambridge, MA USA. [Qiu, Wei Qiao] Boston Univ, Sch Med, Boston, MA 02118 USA. RP Paul, L (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM ligi.paul_pottenplackel@tufts.edu FU USDA [58-1950-4-003]; National Institute on Aging [AG21790-01] FX Supported by the USDA (special cooperative agreement 58-1950-4-003) and the National Institute on Aging (grant AG21790-01). NR 36 TC 1 Z9 1 U1 0 U2 0 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD DEC PY 2016 VL 104 IS 6 BP 1665 EP 1670 DI 10.3945/ajcn.116.139030 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA EE0RV UT WOS:000389287500020 PM 27733392 ER PT J AU Bamberg, J del Rio, A Kinder, D Louderback, L Pavlik, B Fernandez, C AF Bamberg, John del Rio, Alfonso Kinder, David Louderback, Lisbeth Pavlik, Bruce Fernandez, Charles TI Core Collections of Potato (Solanum) Species Native to the USA SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Core collection; Mega-population; Genebank; Diversity ID GENETIC-RESOURCES; EX-SITU; WILD; DIVERSITY; RESISTANCE; SOUTHWEST; SELECTION AB Potato has two wild relatives native to the USA, Solanum jamesii (jam) and S. fendleri (fen). Core collections are a useful tool for genebanks, identifying a ranked minimum number of samples that together encompass most of the total genetic diversity. With diversity measured as presence of AFLP bands, we made core collections for each species such that > 90 % of diversity was captured in a minimum of populations. For fen, bulks containing about 25 % of populations accomplished that standard. For jam, a single "mega-population" at Mesa Verde, CO, consisting of many thousands of plants, was found to encompass 82 % of the AFLP bands detected in all samples across the entire USA range, and adding three more populations captured a total of > 90 %. Core members for both jam and fen with the most diversity originated from the northern part of the range. That suggests that these areas merit more collecting. If resources for germplasm preservation and evaluation are limited, samples in the core collections should take priority. C1 [Bamberg, John; del Rio, Alfonso; Fernandez, Charles] USDA ARS, US Potato Genebank, 4312 Hwy 42, Sturgeon Bay, WI 54235 USA. [Kinder, David] Ohio Northern Univ, Pharmaceut & Biomed Sci, 525 S Main St, Ada, OH 45810 USA. [Louderback, Lisbeth] Univ Utah, Dept Anthropol, Nat Hist Museum Utah, 301 Wakara Way, Salt Lake City, UT 84108 USA. [Pavlik, Bruce] Univ Utah, Dept Conservat Red Butte Garden & Arboretum, 300 Wakara Way, Salt Lake City, UT 84108 USA. RP Bamberg, J (reprint author), USDA ARS, US Potato Genebank, 4312 Hwy 42, Sturgeon Bay, WI 54235 USA. EM John.Bamberg@ars.usda.gov OI Del Rio, Alfonso/0000-0001-8780-747X FU USDA/ARS/PEO Beltsville FX The authors thank USDA/ARS/PEO Beltsville for funding the collecting expeditions. NR 30 TC 0 Z9 0 U1 2 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD DEC PY 2016 VL 93 IS 6 BP 564 EP 571 DI 10.1007/s12230-016-9536-2 PG 8 WC Agronomy SC Agriculture GA ED6DO UT WOS:000388945700004 ER PT J AU Singh, RK Navarre, DA Brown, CR AF Singh, Rajesh K. Navarre, Duroy A. Brown, Charles R. TI Relationship between Sugars and Phenylpropanoids in Tubers from Diverse Genotypes SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Phenylpropanoids; Phenolics; Potatoes; Metabolism; Zebra chip; Sugars; Sucrose ID ANTIOXIDANT; PHENOLICS; POTATOES; METABOLISM; EXPRESSION; ACCUMULATION; ANTHOCYANIN; SUCROSE; PLANT; GENE AB Previous studies suggested sucrose may play a regulatory role in potato phenylpropanoid metabolism via activation of MYB transcription factors, but the interaction between sucrose and phenylpropanoids has not been studied in field grown potatoes. Exogenous sucrose increased phenylpropanoids in plantlets, whereas wounding of tuber samples increased sucrose and phenylpropanoids. Tuber sugars and phenylpropanoids were measured in over 100 different potato samples representing different genotypes, developmental stages and locations. Total phenolic content ranged from 1.6 to 17.0 mg/g dry wt and sucrose concentrations ranged from 4.7 to 132 mg/g dry wt. Sucrose was the most abundant sugar, followed by glucose and fructose. Overall, a modest positive correlation was seen between sucrose and phenolic concentrations (R-2 = 0.55; p < 0.01). Typically, tubers with a higher concentration of sucrose contained higher phenolic levels. When the same cultivars were grown in multiple locations, the tubers with the lowest amount of phenolics also had the lowest amount of sucrose. The higher amounts of phenolics found in immature potatoes relative to mature potatoes also correlated with higher amounts of sucrose. Tubers infected with Zebra chip disease had higher amounts of phenylpropanoids, and also higher amounts of sucrose. These data support a regulatory role for sucrose in tuber phenylpropanoid metabolism and suggest that the absolute amount of tuber sucrose is important, but also changes in tuber sucrose homeostasis. C1 [Singh, Rajesh K.; Navarre, Duroy A.; Brown, Charles R.] Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. [Navarre, Duroy A.; Brown, Charles R.] USDA ARS, Yakima Agr Res Lab, 24106 N Bunn Rd, Prosser, WA 99350 USA. RP Navarre, DA (reprint author), Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA.; Navarre, DA (reprint author), USDA ARS, Yakima Agr Res Lab, 24106 N Bunn Rd, Prosser, WA 99350 USA. EM Roy.navarre@ars.usda.gov FU Washington State Potato Commission; Northwest Potato Research Consortium; NIFA SCRI [2011-51181-30829] FX Thanks to NRSP-6, J.R. Simplot Company, the Western Regional Trial and the TriState Breeding Program for providing potatoes. Thanks to Bernardo Chaves for the SAS analysis. We thank Dr. Andy Jensen, the Washington State Potato Commission and Northwest Potato Research Consortium for support. This research was funded by NIFA SCRI research project 2011-51181-30829. NR 27 TC 0 Z9 0 U1 3 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD DEC PY 2016 VL 93 IS 6 BP 581 EP 589 DI 10.1007/s12230-016-9538-0 PG 9 WC Agronomy SC Agriculture GA ED6DO UT WOS:000388945700006 ER PT J AU Sathuvalli, V Brown, CR Yilma, S Charlton, BA Shock, CC Quick, R Feibert, E Whitworth, JL Novy, RG Stark, JC Pavek, MJ Knowles, NR Navarre, RA Debons, J Vales, MI AF Sathuvalli, V. Brown, C. R. Yilma, S. Charlton, B. A. Shock, C. C. Quick, R. Feibert, E. Whitworth, J. L. Novy, R. G. Stark, J. C. Pavek, M. J. Knowles, N. R. Navarre, R. A. Debons, J. Vales, M. I. TI Yukon Nugget: a Mid-Season Yellow Skin, Yellow Flesh Specialty Potato with Extreme Resistance to Potato Virus X SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Solanum tuberosum; Variety; Breeding; PVX; Gourmet ID COMMON SCAB AB Yukon Nugget is a mid-season specialty potato with yellow flesh, yellow skin and distinct red eyes. Yukon Nugget was developed to provide the potato industry with an alternative to Yukon Gold. The overall tuber size profile of Yukon Nugget is smaller and more uniform than Yukon Gold and it typically produces an average of four more tubers per plant than Yukon Gold. Yukon Nugget tubers are ideal for boiling, baking, and microwaving, and have culinary and nutritional qualities generally similar to Yukon Gold. Yukon Nugget has extreme resistance to Potato Virus X due to presence of PVX resistance allele Rx1. It also has moderate resistance to powdery scab and tuber late blight. Yukon Nugget has less vascular and stem end discoloration and less hollow heart than Yukon Gold. Yukon Nugget is similar to Yukon Gold in terms of susceptibility to several major potato diseases, such as PVY, PLRV, and foliage late blight. It was released in 2013 by the Agricultural Experiment Stations of Oregon, Idaho and Washington and the USDA-ARS, and is a product of the Pacific Northwest (Tri-State) Potato Variety Development Program. C1 [Sathuvalli, V.] Oregon State Univ, Hermiston Agr R&E Ctr, Hermiston, OR 97838 USA. [Brown, C. R.; Quick, R.; Navarre, R. A.] USDA ARS, Prosser, WA 99350 USA. [Yilma, S.; Vales, M. I.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Charlton, B. A.] Oregon State Univ, Klamath Basin R&E Ctr, Klamath Falls, OR 97603 USA. [Shock, C. C.; Feibert, E.] Oregon State Univ, Malheur Expt Stn, Ontario, OR 97914 USA. [Whitworth, J. L.; Novy, R. G.] USDA ARS, Aberdeen R&E Ctr, Aberdeen, ID 83210 USA. [Stark, J. C.] Univ Idaho, Idaho Falls R&E Ctr, Idaho Falls, ID 83402 USA. [Pavek, M. J.; Knowles, N. R.] Washington State Univ, Dept Hort, Pullman, WA 99164 USA. [Debons, J.] PVMI, Bend, OR 97702 USA. RP Sathuvalli, V (reprint author), Oregon State Univ, Hermiston Agr R&E Ctr, Hermiston, OR 97838 USA. EM Vidyasagar@oregonstate.edu NR 9 TC 0 Z9 0 U1 3 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD DEC PY 2016 VL 93 IS 6 BP 602 EP 608 DI 10.1007/s12230-016-9540-6 PG 7 WC Agronomy SC Agriculture GA ED6DO UT WOS:000388945700008 ER PT J AU Ebi, KL Ziska, LH Yohe, GW AF Ebi, Kristie L. Ziska, Lewis H. Yohe, Gary W. TI The shape of impacts to come: lessons and opportunities for adaptation from uneven increases in global and regional temperatures SO CLIMATIC CHANGE LA English DT Article DE Climate change; Adaptation; Global warming hiatus; Surface warming slowdown ID CLIMATE-CHANGE; WARMING HIATUS; AGRICULTURE AB Uneven patterns in the rate of climate change have profound implications for adaptation. Assuming a linear or monotonic increase in global or regional temperatures can lead to inefficient planning processes that underestimate the magnitude, pattern, and timing of the risks faced by human and natural systems, which could exaggerate future impacts and the costs of managing them. Adaptation planning needs to move beyond imposing linear thinking and analysis onto nonlinear systems. Doing so would improve research into adaptive management processes that learn from and adapt to new knowledge at a pace that reflects non-linearity. Specifically, the pace of adaptation must consider the potential consequences of uneven increases in weather and climate variables as a means to reduce system vulnerability. Projections simulating periods of relative stability with those of rapid change would lead to more complex and more accurate expectations of future risks and associated consequences for human and natural systems. Adaptation planning based on such projections could then consider the implications of non-linear climate change on the extent of any adaptation effort, including quantified (or qualitative) risks and associated costs and benefits. Adaptation planning could be improved by projections that incorporate more nuanced understandings of how development processes could interact with climate change to alter future risks and vulnerabilities. Two examples are highlighted to illustrate the complexity and dynamic nature of non-monotonic climate-development-response scenarios: vector borne diseases and agricultural productivity. C1 [Ebi, Kristie L.] Univ Washington, Ctr Hlth & Global Environm, Seattle, WA 98105 USA. [Ziska, Lewis H.] USDA, Beltsville, MD 20705 USA. [Yohe, Gary W.] Wesleyan Univ, Middletown, CT 06459 USA. RP Ebi, KL (reprint author), Univ Washington, Ctr Hlth & Global Environm, Seattle, WA 98105 USA. EM krisebi@uw.edu NR 26 TC 0 Z9 0 U1 16 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0165-0009 EI 1573-1480 J9 CLIMATIC CHANGE JI Clim. Change PD DEC PY 2016 VL 139 IS 3-4 BP 341 EP 349 DI 10.1007/s10584-016-1816-9 PG 9 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA ED6JQ UT WOS:000388962300001 ER PT J AU Polley, HW Gibson, AE Fay, PA Wilsey, BJ AF Polley, H. Wayne Gibson, Anne E. Fay, Philip A. Wilsey, Brian J. TI Biotic Regulation of CO2 Uptake-Climate Responses: Links to Vegetation Properties SO ECOSYSTEMS LA English DT Article DE exotic plant species; grassland; gross primary productivity; net radiation; normalized difference vegetation index; plant traits; precipitation; specific leaf area ID NET ECOSYSTEM CO2; INTERANNUAL VARIABILITY; FUNCTIONAL DIVERSITY; UNITED-STATES; ENVIRONMENTAL VARIATION; MAINTENANCE MECHANISMS; GREAT-PLAINS; EXCHANGE; CARBON; PRECIPITATION AB Identifying the plant traits and patterns of trait distribution in communities that are responsible for biotic regulation of CO2 uptake-climate responses remains a priority for modeling terrestrial C dynamics. We used remotely sensed estimates of gross primary productivity (GPP) from plots planted to different combinations of perennial grassland species in order to determine links between traits and GPP-climate relationships. Climatic variables explained about 50% of the variance in temporal trends in GPP despite large variation in CO2 uptake among seasons, years, and plots of differing composition. GPP was highly correlated with contemporary changes in net radiation (Rn) and precipitation deficit (potential evapotranspiration minus precipitation) but was negatively correlated with precipitation summed over 210 days prior to flux measurements. Plots differed in GPP-Rn and GPP-water (deficit, precipitation) relationships. Accounting for differences in GPP-climate relationships explained an additional 11% of variance in GPP. Plot differences in GPP-Rn and GPP-precipitation slopes were linked to differences in community-level light-use efficiency (GEE*). Plot differences in GPP-deficit slopes were linked to differences in a species abundance-weighted index of specific leaf area (SLA). GEE* and weighted SLA represent vegetation properties that may regulate how CO2 uptake responds to climatic variation in grasslands. C1 [Polley, H. Wayne; Gibson, Anne E.; Fay, Philip A.] USDA Agr Res Serv, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Wilsey, Brian J.] Iowa State Univ, Dept Ecol Evolut & Organismal Biol, 251 Bessey Hall, Ames, IA 50011 USA. RP Polley, HW (reprint author), USDA Agr Res Serv, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. EM wayne.polley@ars.usda.gov FU USDA-NIFA [2014-67003-22067] FX Field and laboratory assistance from Katherine Jones and Chris Kolodziejczyk was invaluable. This work was partially funded by the USDA-NIFA via Grant 2014-67003-22067. Mention of trade names or commercial products does not imply endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 39 TC 0 Z9 0 U1 7 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1432-9840 EI 1435-0629 J9 ECOSYSTEMS JI Ecosystems PD DEC PY 2016 VL 19 IS 8 BP 1376 EP 1385 DI 10.1007/s10021-016-0009-8 PG 10 WC Ecology SC Environmental Sciences & Ecology GA ED5YC UT WOS:000388928800004 ER PT J AU Woodall, CW Walters, BF Russell, MB Coulston, JW Domke, GM D'Amato, AW Sowers, PA AF Woodall, C. W. Walters, B. F. Russell, M. B. Coulston, J. W. Domke, G. M. D'Amato, A. W. Sowers, P. A. TI A Tale of Two Forest Carbon Assessments in the Eastern United States: Forest Use Versus Cover as a Metric of Change SO ECOSYSTEMS LA English DT Article DE forest inventory; climate change; carbon; land use; land-use change; stock change; forest cover ID LAND-USE CHANGE; SOIL CARBON; USE HISTORY; DYNAMICS; SINK; ACCUMULATION; MANAGEMENT; BIOMASS; MODELS AB The dynamics of land-use practices (for example, forest versus settlements) is often a major driver of changes in terrestrial carbon (C). As the management and conservation of forest land uses are considered a means of reducing future atmospheric CO2 concentrations, the monitoring of forest C stocks and stock change by categories of land-use change (for example, croplands converted to forest) is often a requirement of C monitoring protocols such as those espoused by the Intergovernmental Panel on Climate Change (that is, Good Practice Guidance and Guidelines). The identification of land use is often along a spectrum ranging from direct observation (for example, interpretation of owner intent via field visits) to interpretation of remotely sensed imagery (for example, land cover mapping) or some combination thereof. Given the potential for substantial differences across this spectrum of monitoring techniques, a region-wide, repeated forest inventory across the eastern U.S. was used to evaluate relationships between forest land-use change (derived from a forest inventory) and forest cover change (derived from Landsat modeling) in the context of forest C monitoring strategies. It was found that the correlation between forest land-use change and cover change was minimal (< 0.08), with an increase in forest land use but a net decrease in forest cover being the most frequent observation. Cover assessments may be more sensitive to active forest management and/or conversion activities that can lead to confounded conclusions regarding the forest C sink (for example, decreasing forest cover but increasing C stocks in industrial timberlands). In contrast, the categorical nature of direct land-use field observations reduces their sensitivity to forest management activities (for example, clearcutting versus thinning) and recent disturbance events (for example, floods or wildfire) that may obscure interpretation of C dynamics over short time steps. While using direct land-use observations or cover mapping in forest C assessments, they should not be considered interchangeable as both approaches possess idiosyncratic qualities that should be considered when developing conclusions regarding forest C attributes and dynamics across large scales. C1 [Woodall, C. W.; Walters, B. F.; Domke, G. M.; Sowers, P. A.] USDA Forest Serv, Forest Inventory & Anal Program, Northern Res Stn, St Paul, MN 55108 USA. [Russell, M. B.] Univ Minnesota, Dept Forest Resources, St Paul, MN USA. [Coulston, J. W.] USDA Forest Serv, Forest Inventory & Anal Program, Southern Res Stn, Blacksburg, VA USA. [D'Amato, A. W.] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT USA. RP Woodall, CW (reprint author), USDA Forest Serv, Forest Inventory & Anal Program, Northern Res Stn, St Paul, MN 55108 USA. EM cwoodall@fs.fed.us OI Domke, Grant/0000-0003-0485-0355 NR 57 TC 1 Z9 1 U1 8 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1432-9840 EI 1435-0629 J9 ECOSYSTEMS JI Ecosystems PD DEC PY 2016 VL 19 IS 8 BP 1401 EP 1417 DI 10.1007/s10021-016-0012-0 PG 17 WC Ecology SC Environmental Sciences & Ecology GA ED5YC UT WOS:000388928800006 ER PT J AU Prestele, R Alexander, P Rounsevell, MDA Arneth, A Calvin, K Doelman, J Eitelberg, DA Engstrom, K Fujimori, S Hasegawa, T Havlik, P Humpenoder, F Jain, AK Krisztin, T Kyle, P Meiyappan, P Popp, A Sands, RD Schaldach, R Schungel, J Stehfest, E Tabeau, A Van Meijl, H Van Vliet, J Verburg, PH AF Prestele, Reinhard Alexander, Peter Rounsevell, Mark D. A. Arneth, Almut Calvin, Katherine Doelman, Jonathan Eitelberg, David A. Engstrom, Kerstin Fujimori, Shinichiro Hasegawa, Tomoko Havlik, Petr Humpenoeder, Florian Jain, Atul K. Krisztin, Tamas Kyle, Page Meiyappan, Prasanth Popp, Alexander Sands, Ronald D. Schaldach, Ruediger Schuengel, Jan Stehfest, Elke Tabeau, Andrzej Van Meijl, Hans Van Vliet, Jasper Verburg, Peter H. TI Hotspots of uncertainty in land-use and land-cover change projections: a global-scale model comparison SO GLOBAL CHANGE BIOLOGY LA English DT Article DE land-use allocation; land-use change; land-use model uncertainty; map comparison; model intercomparison; model variation ID CLIMATE-CHANGE MITIGATION; CARBON STOCKS; EARTH SYSTEM; IMPACT; AGRICULTURE; TRANSITIONS; SCENARIOS; POLICY; MAPS AB Model-based global projections of future land-use and land-cover (LULC) change are frequently used in environmental assessments to study the impact of LULC change on environmental services and to provide decision support for policy. These projections are characterized by a high uncertainty in terms of quantity and allocation of projected changes, which can severely impact the results of environmental assessments. In this study, we identify hotspots of uncertainty, based on 43 simulations from 11 global-scale LULC change models representing a wide range of assumptions of future biophysical and socioeconomic conditions. We attribute components of uncertainty to input data, model structure, scenario storyline and a residual term, based on a regression analysis and analysis of variance. From this diverse set of models and scenarios, we find that the uncertainty varies, depending on the region and the LULC type under consideration. Hotspots of uncertainty appear mainly at the edges of globally important biomes (e.g., boreal and tropical forests). Our results indicate that an important source of uncertainty in forest and pasture areas originates from different input data applied in the models. Cropland, in contrast, is more consistent among the starting conditions, while variation in the projections gradually increases over time due to diverse scenario assumptions and different modeling approaches. Comparisons at the grid cell level indicate that disagreement is mainly related to LULC type definitions and the individual model allocation schemes. We conclude that improving the quality and consistency of observational data utilized in the modeling process and improving the allocation mechanisms of LULC change models remain important challenges. Current LULC representation in environmental assessments might miss the uncertainty arising from the diversity of LULC change modeling approaches, and many studies ignore the uncertainty in LULC projections in assessments of LULC change impacts on climate, water resources or biodiversity. C1 [Prestele, Reinhard; Eitelberg, David A.; Tabeau, Andrzej; Van Vliet, Jasper; Verburg, Peter H.] Vrije Univ Amsterdam, Dept Earth Sci, Environm Geog Grp, De Boelelaan 1087, NL-1081 HV Amsterdam, Netherlands. [Alexander, Peter; Rounsevell, Mark D. A.] Univ Edinburgh, Sch GeoSci, Drummond St, Edinburgh EH8 9XP, Midlothian, Scotland. [Arneth, Almut] Karlsruhe Inst Technol, Dept Atmospher Environm Res IMK IFU, Kreuzeckbahnstr 19, D-82467 Garmisch Partenkirchen, Germany. [Calvin, Katherine; Kyle, Page] Pacific Northwest Natl Lab, Joint Global Change Res Inst, College Pk, MD 20740 USA. [Doelman, Jonathan; Stehfest, Elke] PBL Netherlands Environm Assessment Agcy, POB 303, NL-3720 AH Bilthoven, Netherlands. [Engstrom, Kerstin] Lund Univ, Dept Geog & Ecosyst Sci, Solvegatan 12, Lund, Sweden. [Fujimori, Shinichiro; Hasegawa, Tomoko] Natl Inst Environm Studies, Ctr Social & Environm Syst Res, 16-2 Onogawa, Tsukuba, Ibaraki 3058506, Japan. [Havlik, Petr; Krisztin, Tamas] Int Inst Appl Syst Anal, Ecosyst Serv & Management Program, A-2361 Laxenburg, Austria. [Humpenoeder, Florian; Popp, Alexander] Potsdam Inst Climate Impact Res PIK, POB 60 12 03, D-14412 Potsdam, Germany. [Jain, Atul K.; Meiyappan, Prasanth] Univ Illinois, Dept Atmospher Sci, 105 S Gregory Ave, Urbana, IL 61801 USA. [Sands, Ronald D.] USDA, Resource & Rural Econ Div, Econ Res Serv, Washington, DC 20250 USA. [Schaldach, Ruediger; Schuengel, Jan] Univ Kassel, Ctr Environm Syst Res, Wilhelmshoher Allee 17, D-34109 Kassel, Germany. [Tabeau, Andrzej; Van Meijl, Hans] Wageningen Univ & Res Ctr, LEI, POB 29703, NL-2502 LS The Hague, Netherlands. [Verburg, Peter H.] Swiss Fed Res Inst WSL, Zurcherstr 111, CH-8903 Birmensdorf, Switzerland. RP Prestele, R (reprint author), Vrije Univ Amsterdam, Dept Earth Sci, Environm Geog Grp, De Boelelaan 1087, NL-1081 HV Amsterdam, Netherlands. EM reinhard.prestele@vu.nl RI Fujimori, Shinichiro/A-1288-2015; Jain, Atul/D-2851-2016; OI Fujimori, Shinichiro/0000-0001-7897-1796; Jain, Atul/0000-0002-4051-3228; Prestele, Reinhard/0000-0003-4179-6204; Alexander, Peter/0000-0001-6010-1186 FU European Research Council under the European Union [603542]; SIGMA [603719]; ERC grant GLOLAND [311819]; European Union [633692] FX The research in this paper has been supported by the European Research Council under the European Union's Seventh Framework Programme project LUC4C (Grant No. 603542), SIGMA (Grant 603719) and ERC grant GLOLAND (no. 311819). This paper contributes to the objectives of the Global Land Project (http://www.globallandproject.org) and under the European Union's Horizon 2020 Programme project SUSFANS (Grant 633692). None of the results reported in this article are the official positions of the organizations named here. Any errors or omissions remain the responsibility of the authors. NR 69 TC 4 Z9 4 U1 20 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD DEC PY 2016 VL 22 IS 12 BP 3967 EP 3983 DI 10.1111/gcb.13337 PG 17 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA EC0TJ UT WOS:000387813300013 PM 27135635 ER PT J AU Novick, KA Oishi, AC Miniat, CF AF Novick, Kimberly A. Oishi, A. Christopher Miniat, Chelcy Ford TI Cold air drainage flows subsidize montane valley ecosystem productivity SO GLOBAL CHANGE BIOLOGY LA English DT Article DE complex terrain; drainage flows; drought; ecosystem respiration; gross ecosystem productivity; microclimate; net ecosystem exchange; net ecosystem productivity ID SOUTHEASTERN UNITED-STATES; CLIMATE-CHANGE; COMPLEX TERRAIN; RESPONSE FUNCTIONS; ALPINE FOREST; TEMPERATURE; CO2; CARBON; RESPIRATION; EXCHANGE AB In mountainous areas, cold air drainage from high to low elevations has pronounced effects on local temperature, which is a critical driver of many ecosystem processes, including carbon uptake and storage. Here, we leverage new approaches for interpreting ecosystem carbon flux observations in complex terrain to quantify the links between macro-climate condition, drainage flows, local microclimate, and ecosystem carbon cycling in a southern Appalachian valley. Data from multiple long-running climate stations and multiple eddy covariance flux towers are combined with simple models for ecosystem carbon fluxes. We show that cold air drainage into the valley suppresses local temperature by several degrees at night and for several hours before and after sunset, leading to reductions in growing season respiration on the order of similar to 8%. As a result, we estimate that drainage flows increase growing season and annual net carbon uptake in the valley by >10% and >15%, respectively, via effects on microclimate that are not be adequately represented in regional- and global-scale terrestrial ecosystem models. Analyses driven by chamber-based estimates of soil and plant respiration reveal cold air drainage effects on ecosystem respiration are dominated by reductions to the respiration of aboveground biomass. We further show that cold air drainage proceeds more readily when cloud cover and humidity are low, resulting in the greatest enhancements to net carbon uptake in the valley under clear, cloud-free (i.e., drought-like) conditions. This is a counterintuitive result that is neither observed nor predicted outside of the valley, where nocturnal temperature and respiration increase during dry periods. This result should motivate efforts to explore how topographic flows may buffer eco-physiological processes from macroscale climate change. C1 [Novick, Kimberly A.] Indiana Univ, Sch Publ & Environm Affairs, 702 N Walnut Grove Ave, Bloomington, IN 47405 USA. [Oishi, A. Christopher; Miniat, Chelcy Ford] US Forest Serv, USDA, Southern Res Stn, Coweeta Hydrol Lab, 3160 Coweeta Lab Rd, Otto, NC 28763 USA. RP Novick, KA (reprint author), Indiana Univ, Sch Publ & Environm Affairs, 702 N Walnut Grove Ave, Bloomington, IN 47405 USA. EM knovick@indiana.edu FU USDA Forest Service Southern Research Station; NSF Long-Term Ecological Research (LTER) program [DEB-0823293, DEB-1440485] FX The authors thank C. Sobek and S. Laseter for assistance with data collection and archiving, and P. Bolstad for providing valuable feedback on an early version of the manuscript. We thank W. Swank, J. Vose, L. Swift, and many other Coweeta Hydrologic Laboratory scientists for the dedicated efforts to support the long-term operation of the climate stations and the installation of the Coweeta Hydrologic Lab flux tower. Funding for the work was provided by the USDA Forest Service Southern Research Station and the NSF Long-Term Ecological Research (LTER) program (DEB-0823293 and DEB-1440485). We are grateful for the data provided by the NC CHRONOS database, a product of the State Climate Office of North Carolina, and for the data provided by the US Climate Reference Network, which is managed and maintained by the National Oceanic and Atmospheric Administration's (NOAA) National Centers for Environmental Information. The authors state they have no financial conflict of interest in the results of this study. NR 53 TC 0 Z9 0 U1 10 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD DEC PY 2016 VL 22 IS 12 BP 4014 EP 4027 DI 10.1111/gcb.13320 PG 14 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA EC0TJ UT WOS:000387813300016 PM 27081931 ER PT J AU de Souza, AR de Araujo, GC Zanphorlin, LM Ruller, R Franco, FC Torres, FAG Mertens, JA Bowman, MJ Gomes, E Da Silva, R AF de Souza, Angelica R. de Araujo, Gabriela C. Zanphorlin, Leticia M. Ruller, Roberto Franco, Fernanda C. Torres, Fernando A. G. Mertens, Jeffrey A. Bowman, Michael J. Gomes, Eleni Da Silva, Roberto TI Engineering increased thermostability in the GH-10 endo-1,4-beta-xylanase from Thermoascus aurantiacus CBMAI 756 SO INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES LA English DT Article DE Protein engineering; Rational design; Endo-xylanase; Site-directed mutagenesis; Thermostability ID TRICHODERMA-REESEI ENDO-1,4-BETA-XYLANASE-II; AMINO-ACID-SEQUENCE; DIRECTED EVOLUTION; INDUSTRIAL APPLICATIONS; THERMOPHILIC PROTEINS; ANGSTROM RESOLUTION; MOLECULAR-DYNAMICS; THERMAL-STABILITY; CRYSTAL-STRUCTURE; ESCHERICHIA-COLI AB The GH10 endo-xylanase from Thermoascus aurantiacus CBMAI 756 (XynA) is industrially attractive due to its considerable thermostability and high specific activity. Considering the possibility of a further improvement in thermostability, eleven mutants were created in the present study via site-directed mutagenesis using XynA as a template. XynA and its mutants were successfully overexpressed in Escherichia coli Rosetta-gami DE3 and purified, exhibiting maximum xylanolytic activity at pH 5 and 65 degrees C. Three of the eleven mutants, Q158R, H209N, and N257D, demonstrated increased thermostability relative to the wild type at 70 degrees C and 75 C.Q158R and N257D were stable in the pH range 5.0-10.0, while WT and H209N were stable from pH 8-10. CD analysis demonstrated that the WT and the three mutant enzymes were expressed in a folded form. H209N was the most thermostable mutant, showing a Tm of 71.3 degrees C. Molecular dynamics modeling analyses suggest that the increase in H209N thermostability may beattributed to a higher number of short helices and salt bridges, which displayed a positive charge in the catalytic core, stabilizing its tertiary structure. (C) 2016 Elsevier B.V. All rights reserved. C1 [de Souza, Angelica R.; de Araujo, Gabriela C.; Franco, Fernanda C.; Gomes, Eleni; Da Silva, Roberto] Sao Paulo State Univ Julio de Mesquita Filho, UNESP, Biochem & Appl Microbiol Lab, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil. [Torres, Fernando A. G.] Univ Brasilia, Dept Cell Biol, Brasilia, DF, Brazil. [Zanphorlin, Leticia M.; Ruller, Roberto] Natl Ctr Res Energy & Mat, CTBE, Brazilian Bioethanol Sci & Technol Lab, BR-13083100 Campinas, SP, Brazil. [Mertens, Jeffrey A.; Bowman, Michael J.] USDA ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. RP Da Silva, R (reprint author), Sao Paulo State Univ Julio de Mesquita Filho, UNESP, Biochem & Appl Microbiol Lab, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil. EM dasilva@ibilce.unesp.br FU Sao Paulo Research Foundation (FAPESP) [2014/04272-8]; FAPESP in Brazil [2011-22461-4, 02/08355-8, 2010/12624-0, 2014/02080-4]; CNPq [487552/2012-1, 306044/2014-5]; FAPESP [2011/17658-3] FX This work was funded by Sao Paulo Research Foundation (FAPESP), with the external scholarship abroad, 2014/04272-8, and the follwing FAPESP grants in Brazil 2011-22461-4, 02/08355-8, 2010/12624-0, 2014/02080-4, and also by CNPq grants 487552/2012-1 and 306044/2014-5. The authors would also like to thank V.B.P. Leite's cluster (FAPESP grant 2011/17658-3) for the computational resources and CNPEM - LAM for the acquisition of the biophysical data. NR 55 TC 0 Z9 0 U1 14 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0141-8130 EI 1879-0003 J9 INT J BIOL MACROMOL JI Int. J. Biol. Macromol. PD DEC PY 2016 VL 93 BP 20 EP 26 DI 10.1016/j.ijbiomac.2016.08.056 PN A PG 7 WC Biochemistry & Molecular Biology; Chemistry, Applied; Polymer Science SC Biochemistry & Molecular Biology; Chemistry; Polymer Science GA ED7ZI UT WOS:000389090900003 PM 27554938 ER PT J AU Tagtow, A AF Tagtow, Angie TI Science-The Cornerstone of Nutrition Policy, Promotion, and Practice SO Journal of the Academy of Nutrition and Dietetics LA English DT Article C1 [Tagtow, Angie] USDA, Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. RP Tagtow, A (reprint author), USDA, Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD DEC PY 2016 VL 116 IS 12 BP 1892 EP 1893 DI 10.1016/j.jand.2016.09.022 PG 2 WC Nutrition & Dietetics SC Nutrition & Dietetics GA ED9QY UT WOS:000389208800003 PM 27886703 ER PT J AU Di Noia, J Monica, D Gray, HL Cullen, KW AF Di Noia, Jennifer Monica, Dorothy Gray, Heewon Lee Cullen, Karen Weber TI The Special Supplemental Nutrition Program for Women, Infants, and Children Fresh Start Randomized Controlled Trial: Baseline Participant Characteristics and Reliability of Measures SO Journal of the Academy of Nutrition and Dietetics LA English DT Article DE Randomized controlled trial; Nutrition education; Special Supplemental Nutrition Program for Women, Infants, and Children (WIC); Fruit and vegetable intake; Farmers' market nutrition program ID VEGETABLE INTAKE; FOOD INSECURITY; UNITED-STATES; FRUIT; ADULTS; QUESTIONNAIRE; CONSUMPTION; BEHAVIORS; HEALTH; INTERVENTION AB Background The Special Supplemental Nutrition Program for Women, Infants, and Children (WIC) Fresh Start (WFS) is a randomized controlled trial of nutrition education to promote farmers' market fruit and vegetable (F/V) purchases and consumption among women enrolled in WIC. Objectives To describe the baseline characteristics (demographics and F/V intake [ including F/V juice]) of WFS participants, compare the characteristics with those of WIC participants in New Jersey and nationwide, and examine the baseline reliability of study measures. Design Cross-sectional. Participants/setting Seven hundred forty-four women served by a New Jerseyebased WIC agency located in a densely populated, urban area. Main outcome measures Demographic characteristics; newly developed measures of farmers' marketerelated knowledge, attitudes, and skills; and validated measures of F/V intake. Statistical analyses Descriptive statistics to characterize the sample. One-sample t and one-sample sign tests to compare the characteristics with reference values. For dietary behaviors, comparisons were with state and national estimates of the frequency and quantity of F/V intake. Results Participants had a mean age of 28.9 +/- 6.8 years and were predominantly Hispanic (59%), US-born (60%), never married (41%), unemployed (62%), receiving assistance other than WIC (70%), and food insecure (55%). Half reported a high school education or less. Higher proportions of WFS participants than WIC participants nationwide were represented among demographic groups at increased risk of inadequate F/V intake. WFS participants consumed more fruit (2.7 cups/day) but less vegetables (1.4 cups/day) than did women nationwide (1.1 and 1.4 cups/day, respectively; P< 0.01). Although participants consumed recommended amounts of fruit, their vegetable intake was below recommended levels. All but two of the measures developed for the study had reliability coefficients at or above 0.60. Conclusions Intervention is warranted to improve participants' vegetable intake. Registered dietitian nutritionists should be aware of F/V intake differences that may require differential intervention strategies. C1 [Di Noia, Jennifer] William Paterson Univ, Dept Sociol, 300 Pompton Rd, Wayne, NJ 07470 USA. [Monica, Dorothy] St Josephs WIC Program, Paterson, NJ USA. [Gray, Heewon Lee] Columbia Univ, Teachers Coll, Dept Hlth & Behav Studies, New York, NY 10027 USA. [Cullen, Karen Weber] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Di Noia, J (reprint author), William Paterson Univ, Dept Sociol, 300 Pompton Rd, Wayne, NJ 07470 USA. EM dinoiaj@wpunj.edu FU US Department of Agriculture, Food and Nutrition Service [WIC NEI-12-TX] FX This project is supported with federal funds from the US Department of Agriculture, Food and Nutrition Service, through grant WIC NEI-12-TX to Baylor College of Medicine to Jennifer Di Noia at William Paterson University. The contents of this publication do not necessarily reflect the view or policies of the US Department of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement by the US government. NR 61 TC 0 Z9 0 U1 9 U2 9 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD DEC PY 2016 VL 116 IS 12 BP 1899 EP 1913 DI 10.1016/j.jand.2016.07.020 PG 15 WC Nutrition & Dietetics SC Nutrition & Dietetics GA ED9QY UT WOS:000389208800005 PM 27663256 ER PT J AU Di Noia, J Cullen, KW Monica, D AF Di Noia, Jennifer Cullen, Karen Weber Monica, Dorothy TI Social Desirability Trait Is Associated with Self-Reported Vegetable Intake among Women Enrolled in the Special Supplemental Nutrition Program for Women, Infants, and Children SO Journal of the Academy of Nutrition and Dietetics LA English DT Article DE Randomized controlled trial; Nutrition education; Special Supplemental Nutrition Program for Women, Infants, and Children (WIC); Fruit and vegetable intake; Social desirability trait ID FRUIT; CONSUMPTION; BIAS AB Background The Special Supplemental Nutrition Program for Women, Infants, and Children (WIC) Fresh Start (WFS) is a randomized controlled trial of nutrition education to promote farmers' market fruit and vegetable (F/V) purchases and consumption among women enrolled in WIC. Objectives Using baseline data from WFS to examine associations between social desirability trait, the tendency to respond in a manner consistent with expected norms, and self-reported F/V intake and to determine whether associations, if found, are moderated by participant characteristics. Design Cross-sectional. Participants/setting Seven hundred forty-four women enrolled in WFS. The setting is a New Jerseyebased WIC agency located in a densely populated urban area. Measures Items assessing participant characteristics, a short form of the MarloweCrowne Social Desirability Scale, and validated measures of the frequency and quantity of F/V intake. Statistical analyses Linear regression analysis to examine associations between social desirability trait and F/V intake and hierarchical regression analysis to test for moderation by participant characteristics of the associations between social desirability trait and F/V intake. Results Social desirability trait was significantly associated with times per day vegetables were reported to have been consumed (beta = 0.08, P =0.03). The association was moderated by breastfeeding status. Among breastfeeding women, social desirability trait was unrelated to reported intake, whereas among non-breastfeeding women, it was positively associated with intake (a 1-unit increase in the social desirability score was associated with a 0.12 increase in times per day vegetables were reported to have been consumed). Conclusions Social desirability trait is associated with self-reported vegetable intake among WIC participants generally and non-breastfeeding participants in particular and should be assessed in these groups. Replication studies with comparative measures of " true intake" are needed to determine whether social desirability trait biases selfreports of vegetable intake or whether those with a high social desirability trait consume vegetables more often. C1 [Di Noia, Jennifer] William Paterson Univ, Dept Sociol, 300 Pompton Rd, Wayne, NJ 07470 USA. [Cullen, Karen Weber] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Monica, Dorothy] St Josephs WIC Program, Paterson, NJ USA. RP Di Noia, J (reprint author), William Paterson Univ, Dept Sociol, 300 Pompton Rd, Wayne, NJ 07470 USA. EM dinoiaj@wpunj.edu FU US Department of Agriculture, Food and Nutrition Service [WIC NEI-12-TX] FX This project is supported with federal funds from the US Department of Agriculture, Food and Nutrition Service through grant WIC NEI-12-TX to Baylor College of Medicine to Jennifer Di Noia at William Paterson University. The contents of this publication do not necessarily reflect the view or policies of the US Department of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement by the US government. NR 31 TC 1 Z9 1 U1 5 U2 5 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD DEC PY 2016 VL 116 IS 12 BP 1942 EP 1950 DI 10.1016/j.jand.2016.07.008 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA ED9QY UT WOS:000389208800009 PM 27665255 ER PT J AU Jahns, L Johnson, LK Scheett, AJ Stote, KS Raatz, SK Subar, AF Tande, D AF Jahns, Lisa Johnson, LuAnn K. Scheett, Angela J. Stote, Kim S. Raatz, Susan K. Subar, Amy F. Tande, Desiree TI Measures of Diet Quality across Calendar and Winter Holiday Seasons among Midlife Women: A 1-Year Longitudinal Study Using the Automated Self-Administered 24-Hour Recall SO Journal of the Academy of Nutrition and Dietetics LA English DT Article DE Season; Holiday; Diet quality; Healthy Eating Index 2010 (HEI-2010); Automated Self-Administered 24-Hour Recall (ASA24) ID WEIGHT-GAIN; NUTRIENT INTAKE; ENERGY-INTAKE; BODY-WEIGHT; MEN; CONSUMPTION; POPULATION; OVERWEIGHT; AMERICANS; RESOURCE AB Background Systematic seasonal bias may confound efforts to estimate usual dietary intake and diet quality. Little is known about dietary quality over the winter holiday season. Objectives The aims of this study were to test for differences in intakes of energy, percentage of energy from macronutrients, fruits and vegetables, and diet quality measured using the Healthy Eating Index 2010 (HEI-2010) by calendar and winter holiday seasons. Design Longitudinal cohort design. Participants/setting Data were derived from the Life in All Seasons study. Two cohorts of women aged 40 to 60 years (N=52) from the greater Grand Forks, ND, area were followed for 1 year each between July 2012 and July 2014. Each woman completed an online diet recall using the Automated Self-Administered 24-Hour Recall every 10 days during the year, with a 92% response rate. Statistical analyses Effects of calendar and winter holiday seasons on intakes of energy, percent energy from macronutrients, HEI-2010 total and component scores, and grams per day of individual fruits and vegetables were tested using mixed linear models. Results The mean total HEI-2010 score was 60.1 +/- 1.4. There were seasonal differences in some HEI-2010 component scores, but not in total scores. More lettuce or mixed lettuce salad was consumed during summer than during winter (P=0.034), and more fresh tomatoes were consumed during summer and fall compared with winter (P=0.001). More corn, berries, peaches and nectarines, and melons (P<0.001) were consumed during summer. There was no seasonal difference in reported intakes of energy (P=0.793). The total HEI-2010 score for dietary intake observed over the winter holiday season was lower than the rest of the year (P<0.001). Reported energy intake was not different (P=0.228). Conclusions In this population, diet quality is significantly lower during the winter holiday period, but mostly consistent by season. Multiple recalls in any season can give a reasonable representation of usual overall diet quality throughout the year. C1 [Jahns, Lisa; Johnson, LuAnn K.; Scheett, Angela J.; Raatz, Susan K.] ARS, USDA, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave N, Grand Forks, ND 58203 USA. [Stote, Kim S.] SUNY Empire State Coll, Hlth Sci, Saratoga Springs, NY 12866 USA. [Subar, Amy F.] NCI, Div Canc Control & Populat Sci, Bethesda, MD 20892 USA. [Tande, Desiree] Univ North Dakota, Dept Nutr & Dietet, Grand Forks, ND 58201 USA. RP Jahns, L (reprint author), ARS, USDA, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave N, Grand Forks, ND 58203 USA. EM lisa.jahns@ars.usda.gov FU US Department of Agriculture (USDA), Agricultural Research Service [USDA 5450-51000-48-00D, USDA 3062-51000-51-00D] FX This study was funded by the US Department of Agriculture (USDA), Agricultural Research Service (USDA 5450-51000-48-00D and USDA 3062-51000-51-00D). The contents of this publication do not necessarily reflect the views or policies of the USDA or the Agricultural Research Service, nor does mention of trade names, commercial products, or organizations imply endorsement from the US government. NR 31 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD DEC PY 2016 VL 116 IS 12 BP 1961 EP 1969 DI 10.1016/j.jand.2016.07.013 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA ED9QY UT WOS:000389208800011 PM 27618574 ER PT J AU Winkler-Moser, JK Martini, S AF Winkler-Moser, Jill K. Martini, Silvana TI 2016: a Year of Change for JAOCS SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Editorial Material C1 [Winkler-Moser, Jill K.] ARS, USDA, Natl Ctr Agr Utilizat Res, Funct Foods Res, Peoria, IL 61604 USA. [Martini, Silvana] Utah State Univ, Dept Nutr Dietet & Food Sci, Logan, UT 84322 USA. RP Winkler-Moser, JK (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, Funct Foods Res, Peoria, IL 61604 USA. EM jill.moser@ars.usda.gov NR 0 TC 0 Z9 0 U1 4 U2 4 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD DEC PY 2016 VL 93 IS 12 BP 1573 EP 1574 DI 10.1007/s11746-016-2912-8 PG 2 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA ED4LK UT WOS:000388819600001 ER PT J AU Bantchev, GB Moser, BR Murray, RE Biresaw, G Hughes, SR AF Bantchev, Grigor B. Moser, Bryan R. Murray, Rex E. Biresaw, Girma Hughes, Stephen R. TI Synthesis and Characterization of Phosphonates from Methyl Linoleate and Vegetable Oils SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Phosphonates; Synthesis; P-31 NMR; Linoleate; Size exclusion chromatography; Radical chain reaction ID TRIBOLOGICAL PROPERTIES; FATTY-ACID; ALKENES AB Phosphonates were synthesized on a medium scale (similar to 200 g) from three lipids-methyl linoleate (MeLin), high-oleic sunflower oil (HOSO) and soybean oil (SBO), and three dialkyl phosphites-methyl, ethyl and n-butyl, using a radical initiator. A staged addition of the lipid and the initiator was used to achieve good yields. Good results were observed with MeLin (94-99% conversions of the double bonds, as determined by NMR, and 83-99% isolated yields) and HOSO (99-100% NMR conversions, 87-96% isolated yields) using tert-butyl perbenzoate as the initiator. With SBO, benzoyl peroxide was used as the initiator, due to its capability to generate radicals at a higher rate at slightly lower temperatures, and thus to shorten the reaction time. Conversions of 91-93% (by NMR) and isolated yields of 80-94% were achieved. The progress of the reaction was monitored with GC-MS. The products were characterized using H-1, C-13 and P-31 NMR, IR and gel permeation chromatography. A prolonged reaction led to some transesterification between the carboxylic and phosphite ester groups. Conditions favoring higher reaction rates led to the formation of more oligomers and benzoate fatty ester byproducts. The benzoate fatty ester byproducts were formed by the attack of a benzoate radical on a double bond. The more double bonds that were present per lipid molecule, the more oligomers were formed: MeLin 2-8%, HOSO 3-9% and SBO 8-29%. C1 [Bantchev, Grigor B.; Moser, Bryan R.; Murray, Rex E.; Biresaw, Girma] ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. [Hughes, Stephen R.] ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. RP Bantchev, GB (reprint author), ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM Grigor.Bantchev@ars.usda.gov OI Bantchev, Grigor/0000-0003-2790-5195 NR 20 TC 0 Z9 0 U1 2 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD DEC PY 2016 VL 93 IS 12 BP 1671 EP 1682 DI 10.1007/s11746-016-2909-3 PG 12 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA ED4LK UT WOS:000388819600010 ER PT J AU Wyatt, VT Strahan, GD Jones, K AF Wyatt, Victor T. Strahan, Gary D. Jones, Kerby TI The Enzymatic Synthesis and Characterization of Disolketal Iminodiacetic Acid (DSIDA) SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Glycerol; Polyesters; Solketal; Biodiesel; Water-soluble ID LIPASE-CATALYZED SYNTHESIS; POLY(VINYL CHLORIDE); ADIABATIC PULSES; PENTAERYTHRITOL; GLYCEROL; ESTERS; STABILIZATION; SPECTROSCOPY; NMR; POLYESTERS AB Disolketal iminodiacetic acid (DSIDA) has been synthesized from the enzyme-catalyzed condensation reaction between derivatives of iminodiacetic acid (IDA) and glycerol. According to all available literature, DSIDA is a novel diester that has never been synthesized. It is a precursor to water-soluble polyhydric alcohols and helps to address the global need of the biodiesel industry to find new uses for glycerol. Reacting diMe-IDA with solketal, a protected glycerol, produced DSIDA in yields as high as 96.4% under optimal reaction conditions of 70 A degrees C and 200 Torr for 24 h. The reaction was monitored using ATR-IR and a validated GC method. ATR-IR monitored the disappearance of the primary solketal alcohol and the appearance of cyclic solketal ether bonds in the molecular backbone of the intermediates and product. Structural analysis of the intermediates and products was performed using two-dimensional NMR, GC, GC-MS and elemental analysis. All spectral data was in agreement with the proposed structures for the chemical reaction. Thermal profiles were determined by TGA to be single-stage decompositions above 160 A degrees C. C1 [Wyatt, Victor T.; Strahan, Gary D.; Jones, Kerby] ARS, Sustainable Biofuels & Coprod Res Unit, USDA, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. RP Wyatt, VT (reprint author), ARS, Sustainable Biofuels & Coprod Res Unit, USDA, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM victor.wyatt@ars.usda.gov NR 44 TC 0 Z9 0 U1 5 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD DEC PY 2016 VL 93 IS 12 BP 1683 EP 1695 DI 10.1007/s11746-016-2911-9 PG 13 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA ED4LK UT WOS:000388819600011 ER PT J AU Martinez, MMR Lopez, MPI Iniguez-Davalos, LI Yuill, T Orlova, MV Reeves, WK AF Ramirez Martinez, Maria M. Ibarra Lopez, M. Pilar Iniguez-Davalos, Luis Ignacio Yuill, Thomas Orlova, Maria V. Reeves, Will K. TI New records of ectoparasitic Acari (Arachnida) and Streblidae (Diptera) from bats in Jalisco, Mexico SO JOURNAL OF VECTOR ECOLOGY LA English DT Article DE Spinturnicidae; Streblidae; Laelapidae; Phyllostomidae; Emballonuridae ID GENUS; SPINTURNICIDAE; PHYLLOSTOMIDAE; PERIGLISCHRUS; MESOSTIGMATA; CHIROPTERA AB Ectoparasites of bats in the Neotropics are diverse and play numerous ecological roles as vectors of microbial pathogens and endoparasites and as food sources for other cave fauna living both on their hosts and in bat roosts. The ectoparasites of bats in Jalisco State of western Mexico have not been as well described as those of other states with recent checklists that have focused primarily on the Yucatan Peninsula. We captured bats from 2011-2015 on the south coast and Sierra de Amula, Jalisco using mist nets, and we removed ectoparasites by hand. We identified 24 species of streblid bat flies and six ectoparasitic mites from bats caught in mist nets. There were an additional eight possibly undescribed species of Streblidae. Our collections extend the known range of species into Jalisco. C1 [Ramirez Martinez, Maria M.] Univ Guadalajara, Dept Salud & Ecol Humana, Guadalajara, Jalisco, Mexico. [Ibarra Lopez, M. Pilar; Iniguez-Davalos, Luis Ignacio] Univ Guadalajara, Dept Ecol & Recursos Nat, Guadalajara, Jalisco, Mexico. [Yuill, Thomas] Dept Pathobiol Sci, Madison, WI USA. [Orlova, Maria V.] Tomsk State Univ, Siberia, Russia. [Reeves, Will K.] USDA, APHIS, Western Compliance Assurance Branch, BRS, Ft Collins, CO 80526 USA. RP Reeves, WK (reprint author), USDA, APHIS, Western Compliance Assurance Branch, BRS, Ft Collins, CO 80526 USA. EM wkreeves@gmail.com RI Orlova, Maria/E-9927-2014 FU Programa de Mejoramiento del Profesorado FX We acknowledge financial support provided by the Programa de Mejoramiento del Profesorado to MMRM, Arturo Moreno for access to his property, Juan Pablo Esparza for drawing maps, and Luis Ignacio Iniguez Davalos and Sicilene Gonzalez Ruvalcaba (Universidad de Guadalajara) for providing some ectoparasites for this study. NR 32 TC 0 Z9 0 U1 6 U2 6 PU SOC VECTOR ECOLOGY PI CORONA PA 1966 COMPTON AVE, CORONA, CA 92881 USA SN 1081-1710 EI 1948-7134 J9 J VECTOR ECOL JI J. Vector Ecol. PD DEC PY 2016 VL 41 IS 2 BP 309 EP 313 DI 10.1111/jvec.12228 PG 5 WC Entomology SC Entomology GA EC7LE UT WOS:000388319300014 PM 27860017 ER PT J AU Corella, D Coltell, O Sorli, JV Estruch, R Quiles, L Martinez-Gonzalez, MA Salas-Salvado, J Castaner, O Aros, F Ortega-Calvo, M Serra-Majem, L Gomez-Gracia, E Portoles, O Fiol, M Espino, JD Basora, J Fito, M Ros, E Ordovas, JM AF Corella, Dolores Coltell, Oscar Sorli, Jose V. Estruch, Ramon Quiles, Laura Angel Martinez-Gonzalez, Miguel Salas-Salvado, Jordi Castaner, Olga Aros, Fernando Ortega-Calvo, Manuel Serra-Majem, Lluis Gomez-Gracia, Enrique Portoles, Olga Fiol, Miquel Diez Espino, Javier Basora, Josep Fito, Montserrat Ros, Emilio Ordovas, Jose M. TI Polymorphism of the Transcription Factor 7-Like 2 Gene (TCF7L2) Interacts with Obesity on Type-2 Diabetes in the PREDIMED Study Emphasizing the Heterogeneity of Genetic Variants in Type-2 Diabetes Risk Prediction: Time for Obesity-Specific Genetic Risk Scores SO NUTRIENTS LA English DT Article DE TCF7L2; type-2 diabetes; obesity; T2D-genetic risk scores; TCF7L2-predictive value; PREDIMED study ID SUSCEPTIBILITY LOCI; MEDITERRANEAN DIET; INSULIN-RESISTANCE; COMMON VARIANTS; BODY-FAT; ASSOCIATION; POPULATION; PREVENTION; TRIAL; MASS AB Nutrigenetic studies analyzing gene-diet interactions of the TCF7L2-rs7903146 C > T polymorphism on type-2 diabetes (T2D) have shown controversial results. A reason contributing to this may be the additional modulation by obesity. Moreover, TCF7L2-rs7903146 is one of the most influential variants in T2D-genetic risk scores (GRS). Therefore, to increase the predictive value (PV) of GRS it is necessary to first see whether the included polymorphisms have heterogeneous effects. We comprehensively investigated gene-obesity interactions between the TCF7L2-rs7903146 C > T polymorphism on T2D (prevalence and incidence) and analyzed other T2D-polymorphisms in a sub-sample. We studied 7018 PREDIMED participants at baseline and longitudinally (8.7 years maximum follow-up). Obesity significantly interacted with the TCF7L2-rs7903146 on T2D prevalence, associations being greater in non-obese subjects. Accordingly, we prospectively observed in non-T2D subjects (n = 3607) that its association with T2D incidence was stronger in non-obese (HR: 1.81; 95% CI: 1.13-2.92, p = 0.013 for TT versus CC) than in obese subjects (HR: 1.01; 95% CI: 0.61-1.66; p = 0.979; p-interaction = 0.048). Accordingly, TCF7L2-PV was higher in non-obese subjects. Additionally, we created obesity-specific GRS with ten T2D-polymorphisms and demonstrated for the first time their higher strata-specific PV. In conclusion, we provide strong evidence supporting the need for considering obesity when analyzing the TCF7L2 effects and propose the use of obesity-specific GRS for T2D. C1 [Corella, Dolores; Sorli, Jose V.; Quiles, Laura; Portoles, Olga] Univ Valencia, Sch Med, Dept Prevent Med & Publ Hlth, Valencia 46010, Spain. [Corella, Dolores; Coltell, Oscar; Sorli, Jose V.; Estruch, Ramon; Quiles, Laura; Angel Martinez-Gonzalez, Miguel; Salas-Salvado, Jordi; Castaner, Olga; Aros, Fernando; Ortega-Calvo, Manuel; Serra-Majem, Lluis; Gomez-Gracia, Enrique; Portoles, Olga; Fiol, Miquel; Diez Espino, Javier; Basora, Josep; Fito, Montserrat; Ros, Emilio; Ordovas, Jose M.] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nutr, Madrid 28029, Spain. [Coltell, Oscar] Univ Jaume 1, Dept Comp Languages & Syst, Sch Technol & Expt Sci, Castellon de La Plana 12071, Spain. [Estruch, Ramon] IDIBAPS, Hosp Clin, Dept Internal Med, Barcelona 08036, Spain. [Angel Martinez-Gonzalez, Miguel] Univ Navarra, Dept Prevent Med & Publ Hlth, Navarra Inst Hlth Res IdisNa, Pamplona 31009, Spain. [Salas-Salvado, Jordi; Basora, Josep] Univ Rovira & Virgili, Human Nutr Unit, Dept Biochem & Biotechnol, IISPV, Reus 43003, Spain. [Castaner, Olga; Fito, Montserrat] Hosp del Mar, Med Res Inst IMIM, Cardiovasc Risk & Nutr Res Grp, Barcelona 08003, Spain. [Aros, Fernando] Hosp Txagorritxu, Dept Cardiol, Vitoria 01009, Spain. [Ortega-Calvo, Manuel] Ctr Salud Palmeritas, Dept Family Med, Dist Sanitario Atenc Primaria Sevilla, Seville 41003, Spain. [Serra-Majem, Lluis] Univ Las Palmas Gran Canaria, Res Inst Biomed & Hlth Sci, Las Palmas Gran Canaria 35001, Spain. [Gomez-Gracia, Enrique] Univ Malaga, Sch Med, Dept Epidemiol, E-29071 Malaga, Spain. [Fiol, Miquel] Hosp Son Espases, Palma Inst Hlth Res IdISPa, Palma De Mallorca 07014, Spain. [Diez Espino, Javier] Univ Navarra, Dept Prevent Med & Publ Hlth, Navarra Inst Hlth Res IdisNA, Serv Navarro Salud Osasunbidea, Pamplona 31009, Spain. [Ros, Emilio] Hosp Clin Barcelona, Inst Invest Biomed August Pi Sunyer IDIBAPS, Lipid Clin, Endocrinol & Nutr Serv, E-08036 Barcelona, Spain. [Ordovas, Jose M.] Tufts Univ, Nutr & Genom Lab, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Ordovas, Jose M.] CNIC, Dept Cardiovasc Epidemiol & Populat Genet, Madrid 28029, Spain. [Ordovas, Jose M.] IMDEA Alimentac, Madrid 28049, Spain. RP Corella, D (reprint author), Univ Valencia, Sch Med, Dept Prevent Med & Publ Hlth, Valencia 46010, Spain.; Corella, D (reprint author), Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nutr, Madrid 28029, Spain. EM dolores.corella@uv.es; oscar.coltell@uji.es; jose.sorli@uv.es; RESTRUCH@clinic.cat; laura.quiles@uv.es; mamartinez@unav.es; jordi.salas@urv.cat; ocastaner@imim.es; aborau@secardiologia.es; 106mayorque104@gmail.com; lluis.serra@ulpgc.es; egomezgracia@uma.es; olga.portoles@uv.es; miguel.fiol@ssib.es; javierdiezesp@ono.com; jbasora.tarte.ics@gencat.cat; MFito@imim.es; EROS@clinic.ub.es; jose.ordovas@tufts.edu RI Salas-Salvado, Jordi/C-7229-2017; OI Salas-Salvado, Jordi/0000-0003-2700-7459; Corella, Dolores/0000-0002-2366-4104 FU Spanish Ministry of Health (Instituto de Salud Carlos III); Ministerio de Economia y Competitividad-Fondo Europeo de Desarrollo Regional [PI051839, PI070240, PI1001407, G03/140, CIBER 06/03, RD06/0045 PI07-0954, CNIC-06, PI11/02505, SAF2009-12304, AGL2010-22319-C03-03, PRX14/00527]; University Jaume I [P1-1B2013-54]; Generalitat Valenciana [AP111/10, AP-042/11, BEST/2015/087, GVACOMP2011-151, ACOMP/2011/145, ACOMP/2012/190, ACOMP/2013/159]; FEDER funds [CB06/03]; U.S. Department of Agriculture-Agricultural Research Service (ARS) [58-1950-4-003]; [JR14/00008] FX This study was funded by the Spanish Ministry of Health (Instituto de Salud Carlos III) and the Ministerio de Economia y Competitividad-Fondo Europeo de Desarrollo Regional (projects PI051839, PI070240, PI1001407, G03/140, CIBER 06/03, RD06/0045 PI07-0954, CNIC-06, PI11/02505, SAF2009-12304, AGL2010-22319-C03-03, and PRX14/00527; contract JR14/00008), by the University Jaume I (Project P1-1B2013-54) and by the Generalitat Valenciana (AP111/10, AP-042/11, BEST/2015/087, GVACOMP2011-151, ACOMP/2011/145, ACOMP/2012/190, and ACOMP/2013/159). CIBEROBN is an initiative of Institute of Health Carlos III of Spain which is supported by FEDER funds (CB06/03). This material is based upon work supported by the U.S. Department of Agriculture-Agricultural Research Service (ARS), under Agreement No. 58-1950-4-003. Dolores Corella acknowledges the collaboration of the Real Colegio Complutense at Harvard University, Cambridge. MA, USA. The PREDIMED study was registered at controlled-trials.com (http://www.controlledtrials.com/ISRCTN35739639; ethics approval by the Institutional Review Board of the Hospital Clinic at Barcelona, Spain, 16/07/2002, under the protocol number "G03/140"). The Institutional Review Board of each participating center approved the study protocol, and all participants provided written informed consent. The Institutional Review Board of the Valencia University (protocol number "G03/140", 22/05/2003) approved the PREDIMED-Valencia study protocol, and all participants provided written informed consent. NR 48 TC 0 Z9 0 U1 2 U2 2 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2072-6643 J9 NUTRIENTS JI Nutrients PD DEC PY 2016 VL 8 IS 12 AR 793 DI 10.3390/nu8120793 PG 18 WC Nutrition & Dietetics SC Nutrition & Dietetics GA ED8TP UT WOS:000389144100041 ER PT J AU Jensen, KB Yan, XB Larson, SR Wang, RRC Robins, JG AF Jensen, Kevin B. Yan, Xuebing Larson, Steve R. Wang, Richard R. -C. Robins, Joseph G. TI Agronomic and genetic diversity in intermediate wheatgrass (Thinopyrum intermedium) SO PLANT BREEDING LA English DT Article DE forage quality; forage yield; genetic diversity; genetic structure ID GLOMERATA L. POPULATIONS; MULTILOCUS GENOTYPE DATA; NUTRITIVE-VALUE; FORAGE QUALITY; REGISTRATION; GRASSES; GROWTH; YIELD; FALL; INFERENCE AB Intermediate wheatgrass [Thinopyrum intermedium (Host) Barkworth & D. R. Dewey] plant introductions (PI) have played a critical role in the development of improved intermediate wheatgrass cultivars. The objective of this study was to characterize a large number of intermediate wheatgrass populations over its native range for dry matter yield (DMY), crude protein (CP), invitro true digestibility (IVTD), neutral detergent fibre (NDF), and rhizome development and genetic diversity and structure to identify superior populations and possible geographical regions in which to collect. Analysis of molecular variance using 643 AFLP bands partitioned 31% of the total genetic variation among these populations with 69% variance detected within populations. Bayesian cluster analysis identified two large groups designated as Asian and European under K=2. In general, European accessions had greater DMY than did Asian accessions. Observed trends were towards greater CP values in populations with Asian ancestry. Selection index values above zero were observed in populations with European ancestry, and the exception was the late-maturing (208days to bloom) populations with Asian ancestry. Plant populations 383551, 383561, 401161, 401173, 440021, 578692 (cv. Tegmar) and 598740 had rhizome scores greater than 7.0. Despite significant differences detected between populations (phi(ST)=0.3136; P<0.001), there was strong indication of admixed co-ancestry or possible gene flow between K=2-9 models. C1 [Jensen, Kevin B.; Larson, Steve R.; Wang, Richard R. -C.; Robins, Joseph G.] Utah State Univ, USDA, ARS, FRRL, Logan, UT 84322 USA. [Yan, Xuebing] Henan Agr Univ, Coll Anim & Vet Sci, Zhengzhou 450002, Peoples R China. RP Jensen, KB (reprint author), Utah State Univ, USDA, ARS, FRRL, Logan, UT 84322 USA. EM kevin.jensen@ars.usda.gov NR 52 TC 0 Z9 0 U1 7 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 EI 1439-0523 J9 PLANT BREEDING JI Plant Breed. PD DEC PY 2016 VL 135 IS 6 BP 751 EP 758 DI 10.1111/pbr.12420 PG 8 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA ED8OX UT WOS:000389131900015 ER PT J AU Sathyanarayana, N Mahesh, S Leelambika, M Jaheer, M Chopra, R Rashmi, KV AF Sathyanarayana, N. Mahesh, S. Leelambika, M. Jaheer, M. Chopra, R. Rashmi, K. V. TI Role of genetic resources and molecular markers in Mucuna pruriens (L.) DC improvement SO Plant Genetic Resources-Characterization and Utilization LA English DT Article DE barcoding; genomics; karyotype; L-Dopa; linkage map; Mucuna pruriens; NGS ID L-DOPA; VAR. UTILIS; TRIBAL PULSE; LINKAGE MAP; CHLOROPLAST-DNA; ANTINUTRITIONAL FACTORS; BIOCHEMICAL-COMPOSITION; INVERTED REPEAT; AFLP MARKERS; VULGARIS L. AB Mucuna pruriens (L.) DC is a tropical legume cover crop with promising nutritional and agronomic potentials. It is also a key source of 3,4 dihydroxy-L-phenylalanine (L-Dopa) - a precursor of dopamine used in the treatment of Parkinson's disease. However, lack of well-characterized germplasm plus poor accessibility to genomic resources has hindered its breeding programs. Furthermore, the cause and effect of various biotic and abiotic stresses impacting yield is also little studied. Systematic collection and evaluation of Indian germplasm by our group revealed presence of a diverse gene pool in India that can support a variety of breeding needs. The stability of L-Dopa trait across environments examined through Genotype and environment (G x E) interaction studies, as well as feasibility check on barcoding and phylogenetic analyses based on karyotype and conserved nuclear and chloroplast genes showed promising outcome. Germplasm screening for select biotic abiotic stresses identified resilient genotypes. Advances in use of DNA markers for diversity analysis, linkage map development, tagging of genes/quantitative trait loci for qualitative and quantitative traits, and progress in genomics are presented. C1 [Sathyanarayana, N.] Sikkim Univ, Dept Bot, Gangtok 737102, India. [Mahesh, S.; Leelambika, M.; Jaheer, M.; Rashmi, K. V.] Sir M Visvesvaraya Inst Technol, Dept Biotechnol, Bangalore 562157, Karnataka, India. [Chopra, R.] USDA ARS, Cropping Syst Res Lab, Lubbock, TX 79415 USA. RP Sathyanarayana, N (reprint author), Sikkim Univ, Dept Bot, Gangtok 737102, India. EM sathyan_dixit@yahoo.in OI CHOPRA, RATAN/0000-0003-2088-3341 FU Department of Science and Technology (DST); Dept. of Biotechnology (DBT) - Govt. of India; Kirkhouse Trust (KHT), and UK FX The authors acknowledge financial support from the Department of Science and Technology (DST) and Dept. of Biotechnology (DBT) - Govt. of India, Kirkhouse Trust (KHT), and UK for these projects. N. S. thanks Sikkim University, Gangtok and Sri Krishnadevaraya Educational Trust (Sri KET), Bangalore, India for the laboratory and field facilities. NR 127 TC 1 Z9 1 U1 2 U2 2 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1479-2621 EI 1479-263X J9 PLANT GENET RESOUR-C JI Plant Genet. Resour.-Charact. Util. PD DEC PY 2016 VL 14 IS 4 BP 270 EP 282 DI 10.1017/S1479262116000071 PG 13 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA EE2PV UT WOS:000389427600004 ER PT J AU Jung, SM Hur, YY Preece, JE Fiehn, O Kim, YH AF Jung, Sung-Min Hur, Youn-Young Preece, John E. Fiehn, Oliver Kim, Young-Ho TI Profiling of Disease-Related Metabolites in Grapevine Internode Tissues Infected with Agrobacterium vitis SO Plant Pathology Journal LA English DT Article DE crown gall; gas chromatography mass spectrometry; grapevine; metabolic profiling; multivariate data analysis ID CROWN-GALL RESISTANCE; CHROMATOGRAPHY-MASS SPECTROMETRY; MEDICAGO-TRUNCATULA; XYLELLA-FASTIDIOSA; RICINUS-COMMUNIS; STRAIN F2/5; RESVERATROL; GENES; METABOLOMICS; ROOTSTOCKS AB Green shoot cuttings of 10 different grapevine species were inoculated with Agrobacterium vitis to find disease-related metabolites in the grapevine. Crown galls formed 60 days after inoculation varied in gall severity (GS) evaluated by gall incidence (GI) and gall diameter (GD), which were classified into three response types as RR (low GI and small GD), SR (high GI and small GD), and SS (high GI and large GD), corresponding to resistant, moderately resistant, and susceptible responses, respectively. In this, 4, 4, and 2 Vitis species were classified into RR, SR, and SS, respectively. Gas chromatography mass spectrometry (GC-MS) analysis of the grapevine stem metabolites with A. vitis infection showed 134 metabolites in various compound classes critically occurred, which were differentially clustered with the response types by the principal component analysis. Multivariate analysis of the metabolite profile revealed that 11 metabolites increased significantly in relation to the response types, mostly at post-inoculation stages, more prevalently (8 metabolites) at two days after inoculation than other stages, and more related to SS (7 metabolites) than RR (3 metabolites) or SR (one metabolite). This suggests most of the disease-related metabolites may be rarely pre-existing but mostly induced by pathogen infection largely for facilitating gall development except stilbene compound resveratrol, a phytoalexin that may be involved in the resistance response. All of these aspects may be used for the selection of resistant grapevine cultivars and their rootstocks for the control of the crown gall disease of the grapevine. C1 [Jung, Sung-Min; Hur, Youn-Young] Rural Dev Adm, Natl Inst Hort & Herbal Sci, Fruit Res Div, Wonju 55365, South Korea. [Preece, John E.] USDA ARS, Natl Clonal Germplasm Repository, Davis, CA 95616 USA. [Fiehn, Oliver] Univ Calif Davis, Dept Mol & Cellular Biol, Davis, CA 95616 USA. [Fiehn, Oliver] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA. [Jung, Sung-Min; Kim, Young-Ho] Seoul Natl Univ, Dept Agr Biotechnol, Seoul 08826, South Korea. [Jung, Sung-Min; Kim, Young-Ho] Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul 08826, South Korea. RP Jung, SM (reprint author), Rural Dev Adm, Natl Inst Hort & Herbal Sci, Fruit Res Div, Wonju 55365, South Korea.; Jung, SM; Kim, YH (reprint author), Seoul Natl Univ, Dept Agr Biotechnol, Seoul 08826, South Korea.; Jung, SM; Kim, YH (reprint author), Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul 08826, South Korea. EM fizzfizz@korea.kr; yhokim@snu.ac.kr FU International Collaborative Research Program, Rural Development Administration, Republic of Korea [PJ0085432015] FX We are honored to thank Dr. Thomas Burr at Cornell University for providing Agrobacterium vitis K306 for the crown gall pathogen inoculation test. I would like to thank Drs. Daniel Kluepfel and Ali McClean for providing the disease test facilities. This work was supported by a grant from the International Collaborative Research Program (No. PJ0085432015), Rural Development Administration, Republic of Korea. NR 54 TC 0 Z9 0 U1 4 U2 4 PU KOREAN SOC PLANT PATHOLOGY PI SUWON PA NATIONAL INST AGRICULTURAL SCIENCE & TECHNOLOGY, PLANT PATHOLOGY DIVISION, SUWON, 441-707, SOUTH KOREA SN 1598-2254 EI 2093-9280 J9 PLANT PATHOLOGY J JI Plant Pathol. J. PD DEC PY 2016 VL 32 IS 6 BP 489 EP + DI 10.5423/PPJ.FT.08.2016.0163 PG 13 WC Agriculture, Multidisciplinary; Plant Sciences SC Agriculture; Plant Sciences GA ED9CV UT WOS:000389168700001 PM 27904455 ER PT J AU Turner, DP Ritts, WD Kennedy, RE Gray, AN Yang, ZQ AF Turner, David P. Ritts, William D. Kennedy, Robert E. Gray, Andrew N. Yang, Zhiqiang TI Regional carbon cycle responses to 25 years of variation in climate and disturbance in the US Pacific Northwest SO Regional Environmental Change LA English DT Article DE Pacific Northwest; Carbon; Climate; Disturbances; Net ecosystem production; Net ecosystem carbon exchange ID WESTERN UNITED-STATES; MOUNTAIN PINE-BEETLE; NET ECOSYSTEM PRODUCTION; FOREST DISTURBANCE; TEMPORAL PATTERNS; DETECTING TRENDS; LANDSCAPE-SCALE; COMPLEX TERRAIN; TREE MORTALITY; DOUGLAS-FIR AB Variation in climate, disturbance regime, and forest management strongly influence terrestrial carbon sources and sinks. Spatially distributed, process-based, carbon cycle simulation models provide a means to integrate information on these various influences to estimate carbon pools and flux over large domains. Here we apply the Biome-BGC model over the four-state Northwest US region for the interval from 1986 to 2010. Landsat data were used to characterize disturbances, and forest inventory data were used to parameterize the model. The overall disturbance rate on forest land across the region was 0.8 % year(-1), with 49 % as harvests, 28 % as fire, and 23 % as pest/pathogen. Net ecosystem production (NEP) for the 2006-2010 interval on forestland was predominantly positive (a carbon sink) throughout the region, with maximum values in the Coast Range, intermediate values in the Cascade Mountains, and relatively low values in the Inland Rocky Mountain ecoregions. Localized negative NEPs were mostly associated with recent disturbances. There was large interannual variation in regional NEP, with notably low values across the region in 2003, which was also the warmest year in the interval. The recent (2006-2010) net ecosystem carbon balance (NECB) was positive for the region (14.4 TgC year(-1)). Despite a lower area-weighted mean NECB, public forestland contributed a larger proportion to the total NECB because of its larger area. Aggregated forest inventory data and inversion modeling are beginning to provide opportunities for evaluating model-simulated regional carbon stocks and fluxes. C1 [Turner, David P.; Ritts, William D.; Yang, Zhiqiang] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Kennedy, Robert E.] Oregon State Univ, Coll Earth Ocean & Atmospher Sci, Corvallis, OR 97331 USA. [Gray, Andrew N.] US Forest Serv, USDA, Pacific Northwest Res Stn, Corvallis, OR 97331 USA. RP Turner, DP (reprint author), Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. EM david.turner@oregonstate.edu; david.ritts@oregonstate.edu; robert.kennedy@oregonstate.edu; andy.gray@oregonstate.edu; zhiqiang.yang@oregonstate.edu FU NASA Terrestrial Ecology Program [NNX12AK59G] FX Support was provided by the NASA Terrestrial Ecology Program (NNX12AK59G). Landsat data from US Geological Survey Eros Data Center, Daymet climate data from the North American Carbon Program (served from the Oak Ridge National Laboratory Biogeochemical Dynamics Distributed Active Archive Center), and plot data from the USDA Forest Service Forest Inventory and Analysis Program were essential to this study. NR 76 TC 0 Z9 0 U1 14 U2 14 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1436-3798 EI 1436-378X J9 REG ENVIRON CHANGE JI Reg. Envir. Chang. PD DEC PY 2016 VL 16 IS 8 SI SI BP 2345 EP 2355 DI 10.1007/s10113-016-0956-9 PG 11 WC Environmental Sciences; Environmental Studies SC Environmental Sciences & Ecology GA ED6IK UT WOS:000388959100016 ER PT J AU Esquivel, JF AF Esquivel, Jesus F. TI Nezara viridula (L.)(1) in Central Texas: I. New Host Plant Associations and Reproductive Status of Adults Encountered Within SO SOUTHWESTERN ENTOMOLOGIST LA English DT Article ID BUG HEMIPTERA-PENTATOMIDAE; IMPROVED VISUALIZATION AB Southern green stink bug, Nezara viridula (L.), and other phytophagous stink bug species continue to plague producers of row crops (e.g., cotton [Gossypium hirsutum L.] and soybean [Glycine max (L.) Merrill]) in Texas and the remainder of the Cotton Belt in the southeastern United States. Developing effective management tactics for N. viridula requires a thorough understanding of factors contributing to continued production of field pest populations. Uncultivated plant species in the absence of cultivated crops are key factors known to support N. viridula. This report identifies 17 new host plant associations (from nine plant families) for N. viridula in Central Texas; 12 of these associations were previously unidentified altogether in Central Texas while five plant species associations were identified in other regions of North America and the world. Also, reproductive status data of adults in the new associations aid in identifying new plant species capable of harboring reproductive adults. The new plant species associated with N. viridula should be monitored for developing populations that might eventually, or simultaneously, infest cultivated crops. C1 [Esquivel, Jesus F.] ARS, USDA, Insect Control & Cotton Dis Res Unit, 2765 F&B Rd, College Stn, TX 77845 USA. RP Esquivel, JF (reprint author), ARS, USDA, Insect Control & Cotton Dis Res Unit, 2765 F&B Rd, College Stn, TX 77845 USA. EM Jesus.Esquivel@ars.usda.gov NR 7 TC 0 Z9 0 U1 4 U2 4 PU SOUTHWESTERN ENTOMOLOGICAL SOC PI DALLAS PA 17360 COIT RD, DALLAS, TX 75252-6599 USA SN 0147-1724 EI 2162-2647 J9 SOUTHWEST ENTOMOL JI Southw. Entomol. PD DEC PY 2016 VL 41 IS 4 BP 895 EP 911 PG 17 WC Entomology SC Entomology GA ED3FB UT WOS:000388733700001 ER PT J AU Greggor, AL Berger-Tal, O Blumstein, DT Angeloni, L Bessa-Gomes, C Blackwell, BF St Clair, CC Crooks, K de Silva, S Fernandez-Juricic, E Goldenberg, SZ Mesnick, SL Owen, M Price, CJ Saltz, D Schell, CJ Suarez, AV Swaisgood, RR Winchell, CS Sutherland, WJ AF Greggor, Alison L. Berger-Tal, Oded Blumstein, Daniel T. Angeloni, Lisa Bessa-Gomes, Carmen Blackwell, Bradley F. St Clair, Colleen Cassady Crooks, Kevin de Silva, Shermin Fernandez-Juricic, Esteban Goldenberg, Shifra Z. Mesnick, Sarah L. Owen, Megan Price, Catherine J. Saltz, David Schell, Christopher J. Suarez, Andrew V. Swaisgood, Ronald R. Winchell, Clark S. Sutherland, William J. TI Research Priorities from Animal Behaviour for Maximising Conservation Progress SO TRENDS IN ECOLOGY & EVOLUTION LA English DT Review ID POPULATION VIABILITY; ECOLOGICAL TRAPS; CLIMATE-CHANGE; ET-AL; WILDLIFE; BIOLOGY; DETERRENTS; FRAMEWORK; SCIENCE; AREAS AB Poor communication between academic researchers and wildlife managers limits conservation progress and innovation. As a result, input from overlapping fields, such as animal behaviour, is underused in conservation management despite its demonstrated utility as a conservation tool and countless papers advocating its use. Communication and collaboration across these two disciplines are unlikely to improve without clearly identified management needs and demonstrable impacts of behavioural-based conservation management. To facilitate this process, a team of wildlife managers and animal behaviour researchers conducted a research prioritisation exercise, identifying 50 key questions that have great potential to resolve critical conservation and management problems. The resulting agenda highlights the diversity and extent of advances that both fields could achieve through collaboration. C1 [Greggor, Alison L.] Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA. [Berger-Tal, Oded; Saltz, David] Ben Gurion Univ Negev, Mitrani Dept Desert Ecol, Midreshet Ben Gurion, Israel. [Blumstein, Daniel T.] Univ Calif Los Angeles, Dept Ecol & Evolutionary Biol, Los Angeles, CA USA. [Angeloni, Lisa; Schell, Christopher J.] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Bessa-Gomes, Carmen] Univ Paris Saclay, Ecol Systemat Evolut, Univ Paris Sud, CNRS,AgroParisTech, Orsay, France. [Blackwell, Bradley F.; Schell, Christopher J.] Wildlife Serv, USDA, Natl Wildlife Res Ctr, Ft Collins, CO USA. [St Clair, Colleen Cassady] Univ Alberta, Biol Sci, Edmonton, AB, Canada. [Crooks, Kevin] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [de Silva, Shermin] Smithsonian Conservat Biol Inst, Front Royal, VA USA. [de Silva, Shermin] Trunk & Leaves Inc, Newtonville, MA USA. [Fernandez-Juricic, Esteban] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. [Goldenberg, Shifra Z.] Save Elephants, Nairobi, Kenya. [Mesnick, Sarah L.] Natl Marine Fisheries Serv, Southwest Fisheries Sci Ctr, Natl Ocean & Atmospher Adm, Washington, DC USA. [Owen, Megan; Swaisgood, Ronald R.] San Diego Zoo Global, Inst Conservat Res, Escondido, CA USA. [Price, Catherine J.] Univ Sydney, Sch Life & Environm Sci, Sydney, NSW, Australia. [Suarez, Andrew V.] Univ Illinois, Dept Anim Biol, Champaign, IL 61820 USA. [Suarez, Andrew V.] Univ Illinois, Dept Entomol, Champaign, IL 61820 USA. [Winchell, Clark S.] US Fish & Wildlife Serv, Conservat Partnerships Program, Carlsbad, CA USA. [Sutherland, William J.] Univ Cambridge, Conservat Sci Grp, Dept Zool, Cambridge, England. RP Greggor, AL (reprint author), Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA. EM Alison.L.Greggor@dartmouth.edu OI Greggor, Alison/0000-0003-0998-618X; Schell, Christopher/0000-0002-2073-9852 NR 84 TC 1 Z9 1 U1 26 U2 26 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0169-5347 J9 TRENDS ECOL EVOL JI Trends Ecol. Evol. PD DEC PY 2016 VL 31 IS 12 BP 953 EP 964 DI 10.1016/j.tree.2016.09.001 PG 12 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA EE2DU UT WOS:000389394700014 PM 27692480 ER PT J AU Leathers, TD Price, NPJ Manitchotpisit, P Bischoff, KM AF Leathers, Timothy D. Price, Neil P. J. Manitchotpisit, Pennapa Bischoff, Kenneth M. TI Production of anti-streptococcal liamocins from agricultural biomass by Aureobasidium pullulans SO WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY LA English DT Article DE Antibacterial; Aureobasidium pullulans; Biomass; Liamocins; Streptococcus sp. ID 5-HYDROXY-2-DECENOYL ESTERS; POLY(BETA-L-MALIC ACID); STREPTOCOCCUS-SUIS; POLYOL LIPIDS; MANNITOL; 3,5-DIHYDROXYDECANOYL; MASTITIS; ARABITOL; XYLANASE; OILS AB Liamocins are unique heavier-than-water "oils'' produced by certain strains of the fungus Aureobasidium pullulans. Liamocins have antibacterial activity with specificity for Streptococcus sp. Previous studies reported that liamocin yields were highest from strains of A. pullulans belonging to phylogenetic clades 8, 9, and 11, cultured on medium containing sucrose. In this study, 27 strains from these clades were examined for the first time for production of liamocins from agricultural biomass substrates. Liamocin yields were highest from strains in phylogenetic clade 11, and yields were higher from cultures grown on sucrose than from those grown on pretreated wheat straw. However, when supplementary enzymes (cellulase, beta-glucosidase, and xylanase) were added, liamocin production on pretreated wheat straw was equivalent to that on sucrose. Liamocins produced from wheat straw were free of the melanin contamination common in sucrose-grown cultures. Furthermore, MALDI-TOF MS analysis showed that liamocins produced from wheat straw were under-acetylated, resulting in higher proportions of the mannitol A1 and B1 species of liamocin, the latter of which has the highest biological activity against Streptococcus sp. C1 [Leathers, Timothy D.; Price, Neil P. J.; Bischoff, Kenneth M.] ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 North Univ St, Peoria, IL 61604 USA. [Manitchotpisit, Pennapa] Illinois State Univ, Sch Biol Sci, Normal, IL 61790 USA. RP Leathers, TD (reprint author), ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 North Univ St, Peoria, IL 61604 USA. EM Tim.Leathers@ars.usda.gov NR 26 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0959-3993 EI 1573-0972 J9 WORLD J MICROB BIOT JI World J. Microbiol. Biotechnol. PD DEC PY 2016 VL 32 IS 12 AR 199 DI 10.1007/s11274-016-2158-5 PG 7 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA ED2YO UT WOS:000388716300009 PM 27757794 ER PT J AU Muturi, EJ Ramirez, JL Rooney, AP Dunlap, C AF Muturi, Ephantus J. Ramirez, Jose L. Rooney, Alejandro P. Dunlap, Chris TI Association between fertilizer-mediated changes in microbial communities and Aedes albopictus growth and survival SO ACTA TROPICA LA English DT Article DE Synthetic fertilizers; Ammonium sulfate; Potassium chloride; Ae. albopictus; Microbiota ID 16S RIBOSOMAL-RNA; ANOPHELES-GAMBIAE DIPTERA; RICE FIELDS; INORGANIC FERTILIZERS; MINERAL FERTILIZERS; TREATMENT WETLANDS; SP NOV.; CULICIDAE; MOSQUITOS; SOIL AB Contamination of aquatic habitats with anthropogenic nutrients has been associated with an increase in mosquito larval populations but the underlying mechanisms remain poorly understood. We examined the individual and combined effects of two synthetic fertilizers (ammonium sulfate and potassium chloride) on Aedes albopictus survival, development time, and sex ratio. The bacterial and fungal communities of water samples from different fertilizer treatments were also characterized by MiSeq sequencing of the 16S rRNA gene (bacteria) and internal transcribed spacer 1 (fungi) and their relationship with mosquito survival and development determined. Mosquitoes from ammonium sulfate treatment had significantly lower survival rates and longer development times compared to those from control, potassium chloride or a mixture of the two fertilizers. Fertilizer treatment had no significant effects on Ae. albopictus sex ratio although ammonium sulfate treatment tended to be more biased towards males relative to the other treatments. There were no significant effects of fertilizer treatment on fungal communities. However, potassium chloride treatments had lower bacterial diversity compared to the other treatments and the bacterial community structure of control and potassium chloride treatments differed significantly from that of ammonium sulfate and a mixture of the two fertilizers. Microbial composition but not diversity was significantly associated with mosquito survival and development. These findings suggest that anthropogenic nutrients can have a profound impact on mosquito survival and development. In addition to any potential direct effects on mosquito physiology, our results suggest that fertilizers can act indirectly by disrupting the microbial communities that provide a critical food resource for mosquito larvae. Published by Elsevier B.V. C1 [Muturi, Ephantus J.; Ramirez, Jose L.; Rooney, Alejandro P.; Dunlap, Chris] USDA ARS, Crop Bioprotect Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. RP Muturi, EJ (reprint author), USDA ARS, Crop Bioprotect Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM Ephantus.Muturi@ars.usda.gov NR 56 TC 1 Z9 1 U1 8 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0001-706X EI 1873-6254 J9 ACTA TROP JI Acta Trop. PD DEC PY 2016 VL 164 BP 54 EP 63 DI 10.1016/j.actatropica.2016.08.018 PG 10 WC Parasitology; Tropical Medicine SC Parasitology; Tropical Medicine GA ED7YD UT WOS:000389087700009 PM 27562215 ER PT J AU Ross, JA Herbert, ME Sowa, SP Frankenberger, JR King, KW Christopher, SF Tank, JL Arnold, JG White, MJ Yen, H AF Ross, Jared A. Herbert, Matthew E. Sowa, Scott P. Frankenberger, Jane R. King, Kevin W. Christopher, Sheila F. Tank, Jennifer L. Arnold, Jeffrey G. White, Mike J. Yen, Haw TI A synthesis and comparative evaluation of factors influencing the effectiveness of drainage water management SO AGRICULTURAL WATER MANAGEMENT LA English DT Review DE Agricultural drainage; Surface runoff; Nitrate-nitrogen; Dissolved reactive phosphorus; Total phosphorus; Water quality ID MIDWESTERN UNITED-STATES; SUBSURFACE DRAINAGE; NITRATE LOSS; NUTRIENT CRITERIA; CROP PRODUCTION; TILE DRAINAGE; DRAINMOD-N; LAKE-ERIE; CORN-BELT; NITROGEN AB Viable large-scale crop production in the United States requires artificial drainage in humid and poorly drained agricultural regions. Excess water removal is generally achieved by installing tile drains that export water to open ditches that eventually flow into streams. Drainage water management (DWM) is a conservation practice that allows farmers to artificially raise the outlet elevation of a field's drain tile and can reduce nutrient loss during wet periods by storing more water in the field. We intended to assess the effectiveness of DWM to reduce drainage discharge and nutrient loads and additionally identify predictor variables that influence DWM effectiveness. We compared managed (i.e., DWM) and free draining records using paired t-tests, and identified factors associated with DWM effectiveness using a multiple linear regression approach. T-test results indicated that DWM was highly effective in reducing drainage water discharge and nutrient losses via drain tiles as tile discharge volumes were reduced on average 46%, while tile nitrate loads were reduced by 48%. In addition, total phosphorus and dissolved reactive phosphorus loads were reduced by 55% and 57%, respectively. Based on regression results, we found that several aspects of farm and tile drain management were associated with DWM effectiveness, while site specific landscape characteristics were less likely to predict effectiveness. While DWM is effective as a conservation practice to reduce discharges of water and nutrients from drain tiles, we also identified several knowledge gaps. Future research should investigate effects of DWM on water and nutrients lost in other pathways such as surface runoff, preferential flow, groundwater recharge and biological uptake, and also focus more attention on phosphorus as there is a paucity of research on this topic. (C) 2016 Elsevier B.V. All rights reserved. C1 [Ross, Jared A.; Herbert, Matthew E.; Sowa, Scott P.] Nature Conservancy, 101 E Grand River Ave, Lansing, MI 48906 USA. [Frankenberger, Jane R.] Purdue Univ, Dept Agr & Biol Engn, 225 S Univ St, W Lafayette, IN 47907 USA. [King, Kevin W.] USDA ARS, Soil Drainage Res Unit, 590 Woody Hayes Dr, Columbus, OH 43210 USA. [Christopher, Sheila F.; Tank, Jennifer L.] Univ Notre Dame, Dept Biol Sci, 192 Galvin Life Sci Ctr, Notre Dame, IN 46556 USA. [Arnold, Jeffrey G.; White, Mike J.] USDA ARS, Grassland Soil & Water Res Lab, 808 East Blackland Rd, Temple, TX 76502 USA. [Yen, Haw] Texas A&M Univ, Blackland Res & Extens Ctr, 720 East Blackland Rd, Temple, TX 76502 USA. RP Ross, JA (reprint author), Nature Conservancy, 101 E Grand River Ave, Lansing, MI 48906 USA. EM jared.ross@tnc.org; mherbert@tnc.org; ssowa@tnc.org; frankenb@purdue.edu; kevin.king@ars.usda.gov; sheila.christopher@nd.edu; tank.1@nd.edu; jeff.arnold@ars.usda.gov; mike.white@ars.usda.gov; hyen@brc.tamus.edu FU University of Michigan Water Center FX The authors wish to thank the University of Michigan Water Center for their financial support of this project. We also wish to thank Dr. Jordi Moya-Larano from the Arid Zone Experimental Station for providing information regarding the use of partial regression plots, as well as Nancy Sammons at the USDA-ARS, Grassland Soil and Water Research Laboratory and Sara Leavitt from The Nature Conservancy for extensive logistical support throughout the process of completing this research. Chris May and Kim Hall from The Nature Conservancy, and two anonymous reviewers also deserve thanks as they provided very helpful insight on earlier versions of this manuscript. NR 60 TC 0 Z9 0 U1 6 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD DEC PY 2016 VL 178 BP 366 EP 376 DI 10.1016/j.agwat.2016.10.011 PG 11 WC Agronomy; Water Resources SC Agriculture; Water Resources GA ED7YC UT WOS:000389087600034 ER PT J AU Mayonde, SG Cron, GV Gaskin, JF Byrne, MJ AF Mayonde, Samalesu G. Cron, Glynis V. Gaskin, John F. Byrne, Marcus J. TI Tamarix (Tamaricaceae) hybrids: the dominant invasive genotype in southern Africa SO BIOLOGICAL INVASIONS LA English DT Article DE AFLP; Invasive; Hybridization; Tamaricaceae; Genetic diversity; Population structure ID BIOLOGICAL-CONTROL AGENT; INTERSPECIFIC HYBRIDIZATION; POPULATION-STRUCTURE; COLEOPTERA-CHRYSOMELIDAE; SCHINUS-TEREBINTHIFOLIUS; BRAZILIAN PEPPERTREE; DIORHABDA-ELONGATA; BRITISH-ISLES; UNITED-STATES; EVOLUTION AB Hybridization is regarded as a rapid mechanism for increasing genetic variation that can potentially enhance invasiveness. Tamarix hybrids appear to be the dominant genotypes in their invasions. Exotic Tamarix are declared invasive in South Africa and the exotic T. chinensis and T. ramosissima are known to hybridize between themselves, and with the indigenous T. usneoides. However, until now, it was not known which species or hybrid is the most prevalent in the invasion. With a biocontrol programme being considered as a way of suppressing the alien Tamarix populations, it is important to document the population genetic dynamics of all species in the region. This investigation sought to identify Tamarix species in southern Africa and their hybrids, describe their population structure, and reveal the geographic origin of the invasive species. To achieve this, nuclear Internal Transcribed Spacer (ITS) sequence data and the multilocus Amplified Fragment Length Polymorphisms (AFLPs) markers were used. Phylogenetic analysis and population genetic structure confirmed the presence of three species in South Africa (T. chinensis, T. ramosissima and T. usneoides) with their hybrids. The indigenous T. usneoides is clearly genetically distant from the alien species T. chinensis and T. ramosissima. Interestingly, the Tamarix infestation in South Africa is dominated (64.7 %) by hybrids between T. chinensis and T. ramosissima. The exotic species match their counterparts from their places of origin in Eurasia, as well as those forming part of the invasion in the US. C1 [Mayonde, Samalesu G.; Cron, Glynis V.; Byrne, Marcus J.] Univ Witwatersrand, Johannesburg, Gauteng, South Africa. [Gaskin, John F.] ARS, USDA, 1500 N Cent Ave, Sidney, MT 59270 USA. RP Mayonde, SG (reprint author), Univ Witwatersrand, Johannesburg, Gauteng, South Africa. EM mayondesam84@gmail.com FU Department of Trade and Industries (DTI); National Research Foundation (NRF) FX This work was funded by the Department of Trade and Industries (DTI) and National Research Foundation (NRF) "THRIP'' awards to Isabel Weiersbye and Edward Witkowski (University of Witwatersrand, Johannesburg) for research with AngloGold Ashanti, South Africa (SA) Region Ltd and Ashanti Goldfields Kilo. Special thanks to Ms Kimberley Mann and the Molecular Ecology lab at the United States Department of Agriculture, Agriculture Research Service, Sidney, Montana, USA for her assistance in developing the AFLP markers. We are grateful to the C.E. Moss Herbarium staffs at the School of Animal Plant and Environmental Sciences, Wits University, Johannesburg for providing the facilities and the assistance needed, and to Ruvimbo Mapaya for assistance with the Bayesian analysis. NR 65 TC 1 Z9 1 U1 4 U2 4 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD DEC PY 2016 VL 18 IS 12 BP 3575 EP 3594 DI 10.1007/s10530-016-1249-4 PG 20 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA ED1BU UT WOS:000388579500016 ER PT J AU Prasifka, JR Hulke, BS AF Prasifka, Jarrad R. Hulke, Brent S. TI Relative susceptibility of sunflower maintainer lines and resistance sources to natural infestations of the banded sunflower moth (Lepidoptera: Tortricidae) SO CANADIAN ENTOMOLOGIST LA English DT Article ID HOMOEOSOMA-ELECTELLUM HULST; GROWTH-STAGES; COCHYLIDAE; LARVAE; PERFORMANCE; HELIANTHUS; ASTERACEAE; ASTERALES; PYRALIDAE; DAMAGE AB The banded sunflower moth, Cochylis hospes Walsingham (Lepidoptera: Tortricidae), is a significant seed-feeding pest of sunflowers (Helianthus Linnaeus; Asteraceae) in North America. Though some wild Helianthus, interspecific crosses, and H. annuus Linnaeus cultivars (which precede hybrid sunflower breeding) have low susceptibility to banded sunflower moth, no apparent effort has been made to evaluate modern inbred lines as a source of resistance for hybrids. In field trials from 2013 to 2015, inbred maintainer (HA) lines, resistant accessions (PI), and unreleased, partially inbred (F-6) lines were evaluated in field trials using natural infestations of banded sunflower moth. Results show greater seed damage to maintainer lines than the other groups, but also significant variability among lines within groups. The best maintainer, HA 207, had approximate to 75% fewer damaged seed than the most susceptible HA lines. Among the resistant PI and unreleased inbred lines, only one entry had significantly less damage than HA 207. While PI 494859 showed banded moth resistance superior to the best inbred lines, its other agronomic liabilities make breeding with the least susceptible maintainer lines appear more practical. A key unknown for any attempts to breed hybrids resistant to banded sunflower moth is how closely damage is correlated between inbred lines and their hybrids. C1 [Prasifka, Jarrad R.; Hulke, Brent S.] ARS, USDA, Northern Crop Sci Lab, 1605 Albrecht Blvd North, Fargo, ND 58102 USA. RP Prasifka, JR (reprint author), ARS, USDA, Northern Crop Sci Lab, 1605 Albrecht Blvd North, Fargo, ND 58102 USA. EM jarrad.prasifka@ars.usda.gov NR 27 TC 0 Z9 0 U1 0 U2 0 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X EI 1918-3240 J9 CAN ENTOMOL JI Can. Entomol. PD DEC PY 2016 VL 148 IS 6 BP 736 EP 741 DI 10.4039/tce.2016.32 PG 6 WC Entomology SC Entomology GA ED3FG UT WOS:000388734300011 ER PT J AU Bosco-Lauth, AM Calvert, AE Root, JJ Gidlewski, T Bird, BH Bowen, RA Muehlenbachs, A Zaki, SR Brault, AC AF Bosco-Lauth, Angela M. Calvert, Amanda E. Root, J. Jeffrey Gidlewski, Tom Bird, Brian H. Bowen, Richard A. Muehlenbachs, Atis Zaki, Sherif R. Brault, Aaron C. TI Vertebrate Host Susceptibility to Heartland Virus SO EMERGING INFECTIOUS DISEASES LA English DT Article ID RIFT-VALLEY FEVER; THROMBOCYTOPENIA SYNDROME VIRUS; HEMORRHAGIC-FEVER; BUNYAVIRIDAE PHLEBOVIRUS; NEUTRALIZING ANTIBODIES; MONOCLONAL-ANTIBODIES; TOSCANA VIRUS; UNITED-STATES; RISK-FACTORS; DC-SIGN AB Heartland virus (HRTV) is a recently described phlebovirus initially isolated in 2009 from 2 humans who had leukopenia and thrombocytopenia. Serologic assessment of domestic and wild animal populations near the residence of 1 of these persons showed high exposure rates to raccoons, white-tailed deer, and horses. To our knowledge, no laboratory-based assessments of viremic potential of animals infected with HRTV have been performed. We experimentally inoculated several vertebrates (raccoons, goats, chickens, rabbits, hamsters, C57BL/6 mice, and interferon-alpha/beta/gamma receptor deficient [Ag129]) mice with this virus. All animals showed immune responses against HRTV after primary or secondary exposure. However, neutralizing antibody responses were limited. Only Ag129 mice showed detectable viremia and associated illness and death, which were dose dependent. Ag129 mice also showed development of mean peak viral antibody titers >8 logio PFU/mL, hemorrhagic hepatic lesions, splenomegaly, and large amounts of HRTV antigen in mononuclear cells and hematopoietic cells in the spleen. C1 [Bosco-Lauth, Angela M.; Calvert, Amanda E.; Brault, Aaron C.] Ctr Dis Control & Prevent, 3156 Rampart Rd,Foothills Campus, Ft Collins, CO 80521 USA. [Bosco-Lauth, Angela M.; Bowen, Richard A.] Colorado State Univ, Ft Collins, CO 80523 USA. [Root, J. Jeffrey; Gidlewski, Tom] USDA, Ft Collins, CO USA. [Bird, Brian H.; Muehlenbachs, Atis; Zaki, Sherif R.] Ctr Dis Control & Prevent, Atlanta, GA USA. RP Brault, AC (reprint author), Ctr Dis Control & Prevent, 3156 Rampart Rd,Foothills Campus, Ft Collins, CO 80521 USA. EM abrault@cdc.gov FU American Society for Microbiology postdoctoral fellowship FX A.M.B.-L. was supported by an American Society for Microbiology postdoctoral fellowship. NR 40 TC 0 Z9 0 U1 2 U2 2 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1080-6040 EI 1080-6059 J9 EMERG INFECT DIS JI Emerg. Infect. Dis PD DEC PY 2016 VL 22 IS 12 BP 2070 EP 2077 DI 10.3201/eid2212.160472 PG 8 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA ED5SP UT WOS:000388912900005 PM 27869591 ER PT J AU Moore, SJ Kunkle, R Greenlee, MHW Nicholson, E Richt, J Hamir, A Waters, WR Greenlee, J AF Moore, S. Jo Kunkle, Robert Greenlee, M. Heather West Nicholson, Eric Richt, Juergen Hamir, Amir Waters, W. Ray Greenlee, Justin TI Horizontal Transmission of Chronic Wasting Disease in Reindeer SO EMERGING INFECTIOUS DISEASES LA English DT Article ID ENVIRONMENTAL SOURCES; SHEEP SCRAPIE; DEER; PRIONS; AGENT; FARM AB We challenged reindeer by the intracranial route with the agent of chronic wasting disease sourced from white-tailed deer, mule deer, or elk and tested for horizontal transmission to naive reindeer. Reindeer were susceptible to chronic wasting disease regardless of source species. Horizontal transmission occurred through direct contact or indirectly through the environment. C1 [Moore, S. Jo; Kunkle, Robert; Nicholson, Eric; Richt, Juergen; Hamir, Amir; Waters, W. Ray; Greenlee, Justin] USDA, Ames, IA USA. [Greenlee, M. Heather West] Iowa State Univ, Ames, IA USA. RP Greenlee, J (reprint author), ARS, Virus & Prion Dis Res Unit, Natl Anim Dis Ctr, USDA, 1920 Dayton Ave, Ames, IA 50010 USA. EM justin.greenlee@ars.usda.gov NR 13 TC 0 Z9 0 U1 9 U2 9 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1080-6040 EI 1080-6059 J9 EMERG INFECT DIS JI Emerg. Infect. Dis PD DEC PY 2016 VL 22 IS 12 BP 2142 EP 2145 DI 10.3201/eid2212.160635 PG 4 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA ED5SP UT WOS:000388912900015 PM 27869594 ER PT J AU Breslow, SJ Sojka, B Barnea, R Basurto, X Carothers, C Charnley, S Coulthard, S Dolsak, N Donatuto, J Garcia-Quijano, C Hicks, CC Levine, A Mascia, MB Norman, K Poe, M Satterfield, T St Martin, K Levin, PS AF Breslow, Sara Jo Sojka, Brit Barnea, Raz Basurto, Xavier Carothers, Courtney Charnley, Susan Coulthard, Sarah Dolsak, Nives Donatuto, Jamie Garcia-Quijano, Carlos Hicks, Christina C. Levine, Arielle Mascia, Michael B. Norman, Karma Poe, Melissa Satterfield, Terre St Martin, Kevin Levin, Phillip S. TI Conceptualizing and operationalizing human wellbeing for ecosystem assessment and management SO ENVIRONMENTAL SCIENCE & POLICY LA English DT Article DE Human wellbeing; Indicators; Ecosystem-based management; Integrated ecosystem assessment; Social-ecological system; Sustainability ID ENVIRONMENTAL-MANAGEMENT; FISHING COMMUNITIES; SOCIAL-SCIENCE; HEALTH; CONSERVATION; INDICATORS; SERVICES; IMPACTS; COASTAL; US AB There is growing interest in assessing the effects of changing environmental conditions and management actions on human wellbeing. A challenge is to translate social science expertise regarding these relationships into terms usable by environmental scientists, policymakers, and managers. Here, we present a comprehensive, structured, and transparent conceptual framework of human wellbeing designed to guide the development of indicators and a complementary social science research agenda for ecosystem-based management. Our framework grew out of an effort to develop social indicators for an integrated ecosystem assessment (IEA) of the California Current large marine ecosystem. Drawing from scholarship in international development, anthropology, geography, and political science, we define human wellbeing as a state of being with others and the environment, which arises when human needs are met, when individuals and communities can at meaningfully to pursue their goals, and when individuals and communities enjoy a satisfactory quality of life. We propose four major social science-based constituents of wellbeing: connections, capabilities, conditions, and cross-cutting domains. The latter includes the domains of equity and. justice, security, resilience, and sustainability, which may be assessed through cross-cutting analyses of other constituents. We outline a process for identifying policy-relevant attributes of wellbeing that can guide ecosystem assessments. To operationalize the framework, we provide a detailed table of attributes and a large database of available indicators, which may be used to develop measures suited to a variety of management needs and social goals. Finally, we discuss four guidelines for operationalizing human wellbeing measures in ecosystem assessments, including considerations for context, feasibility, indicators and research, and social difference. Developed for the U.S. west coast, the framework may be adapted for other regions, management needs, and scales with appropriate modifications. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Breslow, Sara Jo; Norman, Karma; Poe, Melissa; Levin, Phillip S.] NOAA, Northwest Fisheries Sci Ctr, 2725 Montlake Blvd E, Seattle, WA 98112 USA. [Sojka, Brit; Barnea, Raz; Dolsak, Nives] Univ Washington, Sch Marine & Environm Affairs, 3707 Brooklyn Ave NE, Seattle, WA 98105 USA. [Basurto, Xavier] Duke Univ, Nicholas Sch Environm, 135 Duke Marine Lab Rd, Beaufort, NC 28516 USA. [Carothers, Courtney] Univ Alaska Fairbanks, Sch Fisheries & Ocean Sci, Anchorage, AK 99501 USA. [Charnley, Susan] US Forest Serv, USDA, Pacific Northwest Res Stn, 620 SW Main St,Suite 400, Portland, OR 97205 USA. [Charnley, Susan] Northumbria Univ, Dept Social Sci & Languages, Newcastle Upon Tyne NE1 8ST, Tyne & Wear, England. [Donatuto, Jamie] Swinomish Indian Tribal Commun, 11404 Moorage Way, La Conner, WA 98257 USA. [Garcia-Quijano, Carlos] Univ Rhode Isl, Dept Sociol & Anthropol, Kingston, RI 02881 USA. [Hicks, Christina C.] Stanford Univ, Ctr Ocean Solut, Monterey, CA 93940 USA. [Hicks, Christina C.] James Cook Univ, ARC Ctr Excellence Coral Reef Studies, Townsville, Qld 4811, Australia. [Levine, Arielle] San Diego State Univ, Dept Geog, 5500 Campanile Dr, San Diego, CA 92182 USA. [Mascia, Michael B.] Conservat Int, Moore Ctr Sci, 2011 Crystal Dr,Suite 500, Arlington, VA 22202 USA. [Poe, Melissa] Univ Washington, Washington Sea Grant, 3716 Brooklyn Ave NE, Washington, WA 98105 USA. [Satterfield, Terre] Univ British Columbia, Inst Resources Environm & Sustainabil, 429-2202 Main Mall, Vancouver, BC V6T 1Z4, Canada. [St Martin, Kevin] Rutgers State Univ, Dept Geog, Piscataway, NJ 08854 USA. [Breslow, Sara Jo] Univ Washington, Ctr Creat Conservat, Seattle, WA 98195 USA. [Levine, Arielle] NOAA, Coral Reef Conservat Program, SSMC4,1305 East West Hwy, Silver Spring, MD 20910 USA. RP Breslow, SJ (reprint author), NOAA, Northwest Fisheries Sci Ctr, 2725 Montlake Blvd E, Seattle, WA 98112 USA.; Breslow, SJ (reprint author), Univ Washington, Ctr Creat Conservat, Seattle, WA 98195 USA. EM sarajo@uw.edu OI Hicks, Christina/0000-0002-7399-4603 FU National Oceanographic and Atmosphere Administration (NOAA); Washington Sea Grant FX This work was supported by the National Oceanographic and Atmosphere Administration (NOAA), and Washington Sea Grant. NR 77 TC 0 Z9 0 U1 18 U2 18 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1462-9011 EI 1873-6416 J9 ENVIRON SCI POLICY JI Environ. Sci. Policy PD DEC PY 2016 VL 66 BP 250 EP 259 DI 10.1016/j.envsci.2016.06.023 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA ED7YT UT WOS:000389089300027 ER PT J AU Brandt, L Lewis, AD Fahey, R Scott, L Darling, L Swanston, C AF Brandt, Leslie Lewis, Abigail Derby Fahey, Robert Scott, Lydia Darling, Lindsay Swanston, Chris TI A framework for adapting urban forests to climate change SO ENVIRONMENTAL SCIENCE & POLICY LA English DT Article DE Vulnerability; Climate change; Adaptive capacity; Urban forest; Inventory; Chicago ID UNITED-STATES; LAND-USE; TREE; VULNERABILITY; CONSERVATION; BIODIVERSITY; ADAPTATION; HABITAT; CITIES; MODEL AB Planting urban trees and expanding urban forest canopy cover are often considered key strategies for reducing climate change impacts in urban areas. However, urban trees and forests can also be vulnerable to climate change through shifts in tree habitat suitability, changes in pests and diseases, and changes in extreme weather events. We developed a three-step framework for urban forest vulnerability assessment and adaptation that scales from regional assessment to local on-the-ground action. We piloted this framework in the Chicago region in 10 locations representing an urban-exurban gradient across a range of socioeconomic capacities. The majority of trees across a seven-county region had low to moderate vulnerability, but many of the least vulnerable species were nonnative invasive species. Urban forests in the 10 pilot locations ranged in vulnerability largely due to differences in economic and organizational adaptive capacity. Adaptation actions selected in these locations tended to focus on increased biodiversity and restoration of natural disturbance regimes. However, adaptation actions in more developed sites also included incorporating new species or cultivars. Lessons learned from the pilot area can be used to inform future efforts in other urban areas. Published by Elsevier Ltd. C1 [Brandt, Leslie; Swanston, Chris] Forest Serv, USDA, Washington, DC USA. [Lewis, Abigail Derby] Field Museum, Chicago, IL USA. [Fahey, Robert] Univ Connecticut, Storrs, CT USA. [Scott, Lydia; Darling, Lindsay] Morton Arboretum, Lisle, IL USA. RP Brandt, L (reprint author), 1992 Folwell Ave, St Paul, MN 55108 USA. EM lbrandt@fs.fed.us FU US Forest Service, Chicago Wilderness; Chicago Region Trees Initiative FX Thanks to P.D. Shannon and K Marcinkowski for developing maps and figures. L. Iverson, S. Matthews, A. Prasad, M. Peters, A. Bell, S. Still, D. Nowak, and A. Bodine provided tree species habitat suitability modeling projections and other quantitative data for the study area. L. Iverson also provided input on modifying factors developed for tree species adaptive capacity. Funding for this work was provided by the US Forest Service, Chicago Wilderness, and the Chicago Region Trees Initiative. NR 46 TC 0 Z9 0 U1 16 U2 16 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1462-9011 EI 1873-6416 J9 ENVIRON SCI POLICY JI Environ. Sci. Policy PD DEC PY 2016 VL 66 BP 393 EP 402 DI 10.1016/j.envsci.2016.06.005 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA ED7YT UT WOS:000389089300042 ER PT J AU Mendis, GP Weiss, SG Korey, M Boardman, CR Dietenberger, M Youngblood, JP Howarter, JA AF Mendis, Gamini P. Weiss, Sydney G. Korey, Matthew Boardman, Charles R. Dietenberger, Mark Youngblood, Jeffrey P. Howarter, John A. TI Phosphorylated Lignin as a Halogen-Free Flame Retardant Additive for Epoxy Composites SO GREEN MATERIALS LA English DT Article ID ANTIOXIDANT PROPERTIES; RECENT PROGRESS; POLYPROPYLENE AB Sustainable, non-halogenated flame retardants are desired for a variety of industry applications. Lignin, an industrially processed wood derivative, has been examined as a potential sustainable flame retardant additive to polymer systems. Here, the lignin is phosphorylated using a pyridine catalyzed esterification reaction with diphenyl phosphoryl chloride to improve its char forming abilities. The flammability performance of modified lignin-epoxy composites was tested using mass loss calorimetry. With the addition of 10% modified lignin, the peak heat release rate decreased by 40% and the total heat of combustion decreased by 20%. C1 [Mendis, Gamini P.; Korey, Matthew; Youngblood, Jeffrey P.; Howarter, John A.] Purdue Univ, Sch Mat Engn, W Lafayette, IN 47907 USA. [Weiss, Sydney G.; Howarter, John A.] Purdue Univ, Div Environm & Ecol Engn, W Lafayette, IN 47907 USA. [Boardman, Charles R.; Dietenberger, Mark] Forest Prod Lab, Madison, WI 53726 USA. RP Howarter, JA (reprint author), 701 W Stadium Ave,MSE Armstrong Hall, W Lafayette, IN 47907 USA. EM howarter@purdue.edu FU NSF-IGERT: Sustainable Electronics [1144843] FX Many thanks to Laura Hasburgh and Huaping Mo for their assistance and technical advice and the Forest Products Lab for the use of their characterization equipment. G.P.M. and M. K were supported in this work through the NSF-IGERT: Sustainable Electronics (Grant Number 1144843). NR 40 TC 0 Z9 0 U1 16 U2 16 PU ICE PUBLISHING PI WESTMINISTER PA INST CIVIL ENGINEERS, 1 GREAT GEORGE ST, WESTMINISTER SW 1P 3AA, ENGLAND SN 2049-1220 EI 2049-1239 J9 GREEN MATER JI Green Mater. PD DEC PY 2016 VL 4 IS 4 DI 10.1680/jgrma.16.00008 PG 33 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Materials Science, Multidisciplinary; Polymer Science SC Science & Technology - Other Topics; Materials Science; Polymer Science GA EC8LA UT WOS:000388391700002 ER PT J AU Fang, XJ Chen, HJ Gao, HY Yang, HL Li, YL Mao, PC Jin, TZ AF Fang, Xiangjun Chen, Hangjun Gao, Haiyan Yang, Hailong Li, Yunlong Mao, Peicheng Jin, Tony Z. TI Effect of modified atmosphere packaging on microbial growth, quality and enzymatic defence of sanitiser washed fresh coriander SO INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Fresh coriander; modified atmosphere packaging; quality; shelf life; washing ID ESCHERICHIA-COLI O157-H7; ACIDIFIED SODIUM-CHLORITE; LISTERIA-MONOCYTOGENES; SENSORY QUALITY; INACTIVATION KINETICS; SALMONELLA-ENTERICA; ESSENTIAL OIL; DIOXIDE GAS; HOT-AIR; EFFICACY AB Fresh coriander samples were washed in an aqueous chlorine dioxide solution and stored in modified atmosphere packaging (MAP) with air (T0), 4%O-2+3%CO2+93%N-2 (T1); 10%O-2+3%CO2+87%N-2 (T2); 15%O-2+3%CO2+82%N-2 (T3); 20%O-2+3%CO2+77%N-2 (T4) at 4 degrees C for 14days. After 14days, the ascorbic acid contents in samples were 24.8 (T3), 22.4 (T4), 19.6 (T0), 18.2 (T2) and 8.8 (T1) mg/100g; the total phenol contents were 59.9 (T3), 53.8 (T0), 50.1 (T4), 44.8 (T2) and 39.3 (T1) mg gallic acid/100g; and the total chlorophyll contents were 1.60 (T3), 1.52 (T4), 1.05 (T2), 0.91 (T0) and 0.63 (T1) mgg(-1) respectively. T3 treatment resulted in the best colour maintenance, the least bacterial growth, and the highest activities of superoxide dismutase and catalase throughout the storage period as compared to others. The data revealed that the T3 MAP condition in combination of aqueous chlorine dioxide washing significantly (P<0.05) maintained the quality and extended the shelf life of fresh coriander. C1 [Fang, Xiangjun; Chen, Hangjun; Gao, Haiyan] Zhejiang Acad Agr Sci, Inst Food Sci, Hangzhou 310021, Zhejiang, Peoples R China. [Fang, Xiangjun; Chen, Hangjun; Gao, Haiyan] Key Lab Fruits & Vegetables Postharvest & Proc Te, Hangzhou 310021, Zhejiang, Peoples R China. [Yang, Hailong] Wenzhou Univ, Coll Life & Environm Sci, Wenzhou 325035, Peoples R China. [Li, Yunlong] Zhejiang Fomdas Foods Co Ltd, Shaoxing 312500, Peoples R China. [Mao, Peicheng] HaiTong Food Grp Co Ltd, Cixi 315300, Peoples R China. [Jin, Tony Z.] ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. RP Gao, HY (reprint author), Zhejiang Acad Agr Sci, Inst Food Sci, Hangzhou 310021, Zhejiang, Peoples R China.; Gao, HY (reprint author), Key Lab Fruits & Vegetables Postharvest & Proc Te, Hangzhou 310021, Zhejiang, Peoples R China.; Jin, TZ (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM spsghy@163.com; tony.jin@ars.usda.gov FU International Science & Technology Cooperation Program of China [2013DFA31450]; Major Science and Technology Projects of Zhejiang Province [2013C02026] FX This project was conducted in collaboration between Zhejiang Academy of Agricultural Science and USDA-ARS under USDA Cooperative Research Agreement (#58-3K95-3-1631-M-F), and financially supported by International Science & Technology Cooperation Program of China (2013DFA31450) and Major Science and Technology Projects of Zhejiang Province (2013C02026). We thank Honglei Mu, Yongjun Zhou and Qiaoling Zhong for their laboratory assistance and Dr. James Smith for his thoughtful review of this manuscript. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity employer. NR 44 TC 1 Z9 1 U1 4 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0950-5423 EI 1365-2621 J9 INT J FOOD SCI TECH JI Int. J. Food Sci. Technol. PD DEC PY 2016 VL 51 IS 12 BP 2654 EP 2662 DI 10.1111/ijfs.13254 PG 9 WC Food Science & Technology SC Food Science & Technology GA ED0VD UT WOS:000388559500018 ER PT J AU Sun, XL Li, WG Li, J Zu, YG Hse, CY Xie, JL Zhao, XH AF Sun, Xiaoli Li, Wengang Li, Jian Zu, Yuangang Hse, Chung-Yun Xie, Jiulong Zhao, Xiuhua TI y Process optimisation of microwave-assisted extraction of peony (Paeonia suffruticosa Andr.) seed oil using hexane-ethanol mixture and its characterisation SO INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Antioxidant activities; fatty acid compositions; microwave-assisted extraction; mixture solvent extraction; peony seed oil; physicochemical properties ID RESPONSE-SURFACE METHODOLOGY; DIELECTRIC-CONSTANT; ORANGE PEEL; SOLVENT; ACID; POLYPHENOLS; ULTRASOUND; PECTIN; GREEN AB Ethanol and hexane mixture agent microwave-assisted extraction (MAE) method was conducted to extract peony (Paeonia suffruticosa Andr.) seed oil (PSO). The aim of the study was to optimise the extraction for both yield and energy consumption in mixture agent MAE. The highest oil yield (34.49%) and lowest unit energy consumption (14125.4Jg(-1)) were obtained under optimum extraction condition: solid-liquid ratio 0.37gmL(-1), extraction time 3.72min, extraction temperature 80.92 degrees C, ethanol ratio 20.00%. GC-MS results showed that unsaturated fatty acids (UFAs) accounted for 88.60% of total fatty acids in PSO. Moreover, linolenic acid content of 37.35% was the highest UFA and caused PSO to possess good nutrition. PSO in DPPH radical scavenging experiment showed that IC50 value of 28.80 +/- 2.13mgmL(-1) exhibited strong antioxidant property. All experiments proved that mixed solvent MAE is an efficient and promising method to extract PSO. This method can effectively reduce the energy consumption and extraction time. C1 [Sun, Xiaoli; Li, Jian] Northeast Forestry Univ, Mat Sci & Engn Coll, Harbin 150040, Heilongjiang, Peoples R China. [Li, Wengang; Zu, Yuangang; Zhao, Xiuhua] Northeast Forestry Univ, Minist Educ, Key Lab Forest Plant Ecol, Harbin 150040, Heilongjiang, Peoples R China. [Hse, Chung-Yun] USDA Forest Serv, Forest Prod Utilizat So Res Stn, Pineville, LA 71360 USA. [Xie, Jiulong] Louisiana State Univ Agr Ctr, Sch Renewable Nat Resources, Louisiana Forest Prod Dev Ctr, Baton Rouge, LA 70803 USA. RP Zhao, XH (reprint author), Northeast Forestry Univ, Minist Educ, Key Lab Forest Plant Ecol, Harbin 150040, Heilongjiang, Peoples R China. EM xiuhuazhao@nefu.edu.cn FU Special Fund for Forestry Scientific Research in the Public Interest [20140470102]; Heilongjiang Postdoctoral Grant [LBH-Z14016] FX The authors acknowledge the financial support from the Special Fund for Forestry Scientific Research in the Public Interest (20140470102) and Heilongjiang Postdoctoral Grant (LBH-Z14016). The authors are also grateful for the precious comments and careful corrections made by anonymous reviewers. NR 30 TC 0 Z9 0 U1 15 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0950-5423 EI 1365-2621 J9 INT J FOOD SCI TECH JI Int. J. Food Sci. Technol. PD DEC PY 2016 VL 51 IS 12 BP 2663 EP 2673 DI 10.1111/ijfs.13255 PG 11 WC Food Science & Technology SC Food Science & Technology GA ED0VD UT WOS:000388559500019 ER PT J AU Cane, JH AF Cane, James H. TI Adult pollen diet essential for egg maturation by a solitary Osmia bee SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Helianthus; Foraging; Reproduction; Vitellogenesis; Apoidea; Yolk ID APIS-MELLIFERA-CARNICA; HYMENOPTERA; CONSUMPTION; VITELLOGENESIS; MEGACHILIDAE; SYRPHIDAE; APOIDEA; NECTAR AB Reproduction is a nutritionally costly activity for many insects, as their eggs are rich in lipids and proteins. That cost seems especially acute for non-social bees, which for their size, lay enormous eggs. All adult female bees visit flowers, most of them to collect pollen and nectar, or sometimes oils, to feed their progeny. For adult bees, the need for pollen feeding has only been detailed for the honey bee, Apis mellifera. To experimentally test for the reproductive value of adult pollen feeding by a non-social bee, Osmia californica (Hymenoptera: Apiformes: Megachilidae), young female bees plus males were released into large glasshouse cages provided with either a male-fertile sunflower cultivar or a pollen-less one. Females regularly visited and drank nectar from flowers of both cultivars. Abundant orange pollen was seen regularly in guts of females confined with the male-fertile sunflowers, indicative of active pollen ingestion. All females' terminal oocytes (next egg to be laid) were small at emergence. Oocytes of females confined with the pollen-less sunflowers remained small, despite frequent nectaring and exposure to other floral stimuli. In contrast, the basal oocytes of female O. californica with access to pollen had swelled to full size within ten days following emergence, enabling them to lay eggs in provided nest tubes. Adult females of this solitary bee required dietary pollen to reproduce; nitrogen stores acquired as larvae were inadequate. Early and regular pollen feeding in part paces the onset and maximum tempo of solitary bees' lifetime reproductive output. C1 [Cane, James H.] Utah State Univ, USDA ARS, Pollinating Insect Res Unit, Logan, UT 84322 USA. RP Cane, JH (reprint author), Utah State Univ, USDA ARS, Pollinating Insect Res Unit, Logan, UT 84322 USA. EM jim.cane@ars.usda.gov NR 38 TC 0 Z9 0 U1 16 U2 16 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 EI 1879-1611 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD DEC PY 2016 VL 95 BP 105 EP 109 DI 10.1016/j.jinsphys.2016.09.011 PG 5 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA ED8GM UT WOS:000389110000011 PM 27659135 ER PT J AU Tooley, PW Browning, M AF Tooley, Paul W. Browning, Marsha TI The Effect of Exposure to Decreasing Relative Humidity on the Viability of Phytophthora ramorum sporangia SO JOURNAL OF PHYTOPATHOLOGY LA English DT Article DE asexual spore; ramorum blight ID POTATO LATE BLIGHT; SUDDEN OAK DEATH; DETACHED SPORANGIA; DOWNY MILDEW; CALIFORNIA; INFESTANS; GERMINATION; PATHOGEN; SURVIVAL; TEMPERATURE AB Sporangia of three isolates of Phytophthora ramorum representing three different clonal lineages were subjected to relative humidity (RH) levels between 80 and 100% for exposure periods ranging from 1 to 24h at 20 degrees C in darkness. Plastic containers (21.5x14.5x5cm) were used as humidity chambers with 130ml of glycerine solution added to each container. Glycerine concentrations corresponded to 100, 95, 90, 85 and 80% RH based on refractive index measurements. Sporangia suspensions were pipeted onto nitrile mesh squares (1.5x1.5cm, 15 micron pore size) which were placed in the humidity chambers and incubated at 20 degrees C in darkness. Following exposure periods of 1, 2, 4, 8, 12 and 24h, mesh squares were inverted onto Petri dishes of selective medium and sporangia germination assessed after 24 and 48h. At 100% RH, we observed a mean value of 88% germination after 1h exposure declining to 18% germination following 24h incubation. At 95% RH, a steeper decline in germination was noted, with means ranging from 79% at 1h to less than 1% at 24h exposure. At 90% RH, no germination was noted after 8 or more h exposure, and values were 57%, 22% and 3% germination for the 1, 2 and 4h exposures, respectively. Germination was only observed at 1h exposure for both the 85% RH treatment (52% germination) and the 80% RH treatment (38% germination). The three isolates responded similarlyover the range of RH values tested. The germination response of P.ramorum sporangia to RH values between 80% and 100% was comparable to that reported for other Phytophthora species. Knowledge of conditions that affect P.ramorum sporangia germination can shed light on pathogenesis and epidemic potential and lead to improved control recommendations. C1 [Tooley, Paul W.; Browning, Marsha] USDA ARS, Foreign Dis Weed Sci Res Unit, 1301 Ditto Ave, Ft Detrick, MD 21702 USA. RP Tooley, PW (reprint author), USDA ARS, Foreign Dis Weed Sci Res Unit, 1301 Ditto Ave, Ft Detrick, MD 21702 USA. EM paul.tooley@ars.usda.gov NR 40 TC 0 Z9 0 U1 6 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-1785 EI 1439-0434 J9 J PHYTOPATHOL JI J. Phytopathol. PD DEC PY 2016 VL 164 IS 11-12 BP 874 EP 881 DI 10.1111/jph.12506 PG 8 WC Plant Sciences SC Plant Sciences GA EC7LB UT WOS:000388319000003 ER PT J AU Rawat, N Pumphrey, MO Liu, SX Zhang, XF Tiwari, VK Ando, K Trick, HN Bockus, WW Akhunov, E Anderson, JA Gill, BS AF Rawat, Nidhi Pumphrey, Michael O. Liu, Sixin Zhang, Xiaofei Tiwari, Vijay K. Ando, Kaori Trick, Harold N. Bockus, William W. Akhunov, Eduard Anderson, James A. Gill, Bikram S. TI Wheat Fhb1 encodes a chimeric lectin with agglutinin domains and a pore-forming toxin-like domain conferring resistance to Fusarium head blight SO NATURE GENETICS LA English DT Article ID QUANTITATIVE TRAIT LOCI; MAJOR QTL; GENE; DEOXYNIVALENOL; GRAMINEARUM; CLONING; BARLEY AB Fusarium head blight (FHB), caused by Fusarium graminearum, is a devastating disease of wheat and barley that leads to reduced yield and mycotoxin contamination of grain, making it unfit for human consumption. FHB is a global problem, with outbreaks in the United States, Canada, Europe, Asia and South America. In the United States alone, total direct and secondary economic losses from 1993 to 2001 owing to FHB were estimated at $7.67 billion(1). Fhb1 is the most consistently reported quantitative trait locus (QTL) for FHB resistance breeding. Here we report the map-based cloning of Fhb1 from a Chinese wheat cultivar Sumai 3. By mutation analysis, gene silencing and transgenic overexpression, we show that a pore-forming toxin-like (PFT) gene at Fhb1 confers FHB resistance. PFT is predicted to encode a chimeric lectin with two agglutinin domains and an ETX/MTX2 toxin domain. Our discovery identifies a new type of durable plant resistance gene conferring quantitative disease resistance to plants against Fusarium species. C1 [Rawat, Nidhi; Tiwari, Vijay K.; Gill, Bikram S.] Kansas State Univ, Dept Plant Pathol, Wheat Genet Resource Ctr, Throckmorton Hall, Manhattan, KS 66506 USA. [Pumphrey, Michael O.; Ando, Kaori] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Liu, Sixin] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV USA. [Zhang, Xiaofei; Anderson, James A.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN USA. [Trick, Harold N.; Bockus, William W.; Akhunov, Eduard] Kansas State Univ, Dept Plant Pathol, Throckmorton Hall, Manhattan, KS 66506 USA. [Rawat, Nidhi; Tiwari, Vijay K.] Univ Maryland, Plant Sci & Landscape Architecture Dept, College Pk, MD 20742 USA. RP Gill, BS (reprint author), Kansas State Univ, Dept Plant Pathol, Wheat Genet Resource Ctr, Throckmorton Hall, Manhattan, KS 66506 USA. EM bsgill@ksu.edu FU US Wheat and Barley Scab Initiative (USDA-ARS) [59-0206-2-088]; US National Science Foundation [IIP-1338897] FX This work was supported by US Wheat and Barley Scab Initiative (USDA-ARS grant 59-0206-2-088) and US National Science Foundation grant contract (IIP-1338897). We thank G. Bai for (USDA-ARS, Manhattan, Kansas) providing seeds of wheat cultivars and landraces for association studies and S. McCormick (USDA-ARS, Peoria, Illinois) for providing purified DON for inoculation experiments. We thank A. Akhunova, R. Matniyazov, S. Sehgal, S. Simsek, K. Benson, B. Friebe, D. Wilson, J.W. Raupp, N. Tyagi, U.M. Quraishi and L. Jiarui for technical support. We acknowledge help from student workers C. McDaniel, K. Madden and R. Clay-Pettis. This is contribution 16-147-J from the Kansas Agricultural Experiment Station. NR 28 TC 1 Z9 1 U1 26 U2 26 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1061-4036 EI 1546-1718 J9 NAT GENET JI Nature Genet. PD DEC PY 2016 VL 48 IS 12 BP 1576 EP 1580 DI 10.1038/ng.3706 PG 5 WC Genetics & Heredity SC Genetics & Heredity GA ED7BQ UT WOS:000389011100020 PM 27776114 ER PT J AU Shrewsbury, LH Smith, JL Huggins, DR Carpenter-Boggs, L Reardon, CL AF Shrewsbury, Lia H. Smith, Jeffrey L. Huggins, David R. Carpenter-Boggs, Lynne Reardon, Catherine L. TI Denitrifier abundance has a greater influence on denitrification rates at larger landscape scales but is a lesser driver than environmental variables SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article ID AMMONIA-OXIDIZING ARCHAEA; LONG-TERM FERTILIZATION; NITROUS-OXIDE; NITRITE REDUCTASE; NO-TILLAGE; SOIL; COMMUNITIES; EMISSIONS; BACTERIA; N2O AB Nitrous oxide is a potent greenhouse gas and its production is mediated by the soil microbial processes of denitrification and nitrification. A thorough understanding of denitrification drivers is necessary to accurately predict and manage nitrous oxide emissions. However, studies disagree on the utility of quantifying the denitrifier community to predict denitrification rates. This study examines the influence of nitrite reductase gene (nirK and nirS) abundance on denitrification rates across a topographically sloping region. The study was carried out over different seasons (autumn, winter, spring, summer) and topographic positions (shoulder and backslope) within a field cropped to spring wheat. A footslope cropped to winter wheat was included in certain analyses to introduce additional variation at a broader scale. We measured denitrification enzyme activity (DEA) and basal denitrification (BD), nirK and nirS abundance, bacterial and archaeal ammonia monooxygenase gene (amoA) abundance, and soil environmental and chemical characteristics which included gravimetric water content, pH, electrical conductivity, and concentrations of nitrate (NO3-N), ammonium (NH4-N), total nitrogen, total soluble nitrogen, total carbon and soluble non-purgeable organic carbon (NPOC). Stepwise multivariate regression (SMR) models of DEA and BD were performed using the measured soil characteristics and denitrifier and nitrifier abundance as explanatory factors to determine whether the microbial community size influenced prediction of denitrification activity. Two SMR models were generated: one SMR model was that with the greatest R-2 from utilizing 1-3 significant variables (P < 0.05) and another model was developed solely from a few proposed primary drivers of denitrification, which we described as soluble NPOC, total soluble N, and denitrifier abundance. The SMR models were also generated at two different landscape scales (with and without footslope). We found nirS abundance to be a significant explanatory variable (P < 0.05) of DEA rates when footslope samples were included in the analysis, explaining 9% and 16% of variation depending upon whether soil gravimetric water content (greatest R2 model) or soluble NPOC (proposed drivers model) was accepted as the primary explanatory variable, respectively. This was however lower than the variance explained by environmental variables (53% and 37%, respectively). When only shoulder and backslope samples were included in the analysis, nirS abundance was only a significant explanatory variable of DEA rates when soluble NPOC (proposed drivers model) was the primary explanatory variable. The proposed drivers models that included soluble NPOC, soluble total N, and nirS population size consistently produced robust predictive models of DEA at the two different field scales (R-2 = 0.53 and 0.70 compared to our greatest-R-2 models of R-2 = 0.61 and 0.79, with and without footslope inclusion, respectively). The nirK abundance was insignificant in the stepwise multivariate regression models of DEA, although it was significantly correlated to DEA (r = 0.35, P = 0.003) when footslope samples were included in the analysis. The nirK nirS abundance was found to be a significant explanatory variable of BD rates (explaining 5% of variance) with footslope inclusion, but total soluble nitrogen concentration explained much more variance (43%). Although we found that denitrifier abundance can be a significant predictor of denitrification rates at the field scale, we conclude that measurements of soil moisture, carbon and nitrogen availability are more useful. Additionally, our study gives evidence that greater landscape variation results in a stronger correlation between denitrification rates and denitrifier abundance, indicating that measurements of the denitrifier community are likely important and useful predictors of denitrification rates at larger scales. (C) Published by Elsevier Ltd. C1 [Shrewsbury, Lia H.; Carpenter-Boggs, Lynne] Washington State Univ, Dept Crop & Soil Sci, POB 646420, Pullman, WA 99164 USA. [Smith, Jeffrey L.; Huggins, David R.] Washington State Univ, USDA ARS, Northwest Sustainable Agroecosyst Res Unit, 215 Johnson Hall, Pullman, WA 99164 USA. [Reardon, Catherine L.] USDA ARS, Columbia Plateau Conservat Res Ctr, 48037 Tubbs Ranch Rd, Adams, OR 97810 USA. RP Reardon, CL (reprint author), USDA ARS, Columbia Plateau Conservat Res Ctr, 48037 Tubbs Ranch Rd, Adams, OR 97810 USA. EM Catherine.Reardon@ars.usda.gov FU USDA-NIFA [2011-67003-3034, 2011-68002-30191] FX With sincere gratitude, the authors appreciate the significant contributions of Dr. Jeffrey L. Smith whose insight, mentorship, and profound collegial nature were integral to this work. The authors would also like to acknowledge the technical assistance provided by Debbie Bikfasy, Elizabeth Torres and Jacob Kollen. This research was supported by USDA-NIFA Award Nos. 2011-67003-3034 and 2011-68002-30191. NR 42 TC 0 Z9 0 U1 29 U2 29 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD DEC PY 2016 VL 103 BP 221 EP 231 DI 10.1016/j.soilbio.2016.08.016 PG 11 WC Soil Science SC Agriculture GA ED3VA UT WOS:000388775400022 ER PT J AU Clark, KL Branch, LC Hierro, JL Villarreal, D AF Clark, Kenneth L. Branch, Lyn C. Hierro, Jose L. Villarreal, Diego TI Burrowing herbivores alter soil carbon and nitrogen dynamics in a semi-arid ecosystem, Argentina SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Burrowing mammals; Vizcachas; Nitrogen mineralization; Plant-available nitrogen; Bioturbation; Ecosystem engineers ID VISCACHA LAGOSTOMUS-MAXIMUS; THOMOMYS-TALPOIDES; CHIHUAHUAN DESERT; LARREA-TRIDENTATA; POCKET GOPHERS; MAMMALS; WATER; VEGETATION; MOUNDS; PLANTS AB Activities of burrowing herbivores, including movement of soil and litter and deposition of waste material, can alter the distribution of labile carbon (C) and nitrogen (N) in soil, affecting spatial patterning of nutrient dynamics in ecosystems where they are abundant. Their role in ecosystem processes in surface soil has been studied extensively, but effects of burrowing species on processes in subsurface soil remain poorly known. We investigated the effects of burrowing and grazing by plains vizcachas (Lagostomus maximus, Chinchilidae), a large colonial burrowing rodent native to South America, on the distribution and dynamics of C and N in soil of a semi-arid scrub ecosystem in central Argentina. In situ N mineralization (N-min), potential N-min and CO2 emissions were measured in surface soil (0-10 cm) and soil at the mean depth of burrows (65 +/- 10 cm; mean +/- 1 SD) in five colonial burrow systems and adjacent grazed and ungrazed zones. Decomposition and N dynamics of vizcacha feces on the soil surface and in burrow soil was assessed using litterbags. Total C and N in soil in burrows were 1.6 and 5.5 times greater than in undisturbed soil at similar depths, and similar to amounts in surface soil. Inorganic N, particularly NO3-, was consistently highest in burrows, intermediate in surface soil on burrow systems, and relatively low in all other zones. Despite high C and N content in all burrows, in situ net Nmin was highly variable in burrow soil. Feces decomposed and released N more rapidly in burrow soil. Laboratory incubations indicated that soil moisture limited Nmin under conditions that typically characterize burrow microclimate, and that rates increased dramatically at soil moisture contents >25% field capacity, which likely occurs during pulsed rainfall events. Thus, the high and seasonally stable NOT content in burrow soil likely originated from the accumulation of pulsed mineralization events over time. Burrowing and waste deposition by vizcachas produced "resource islands" at the landscape scale. At a measured density of 0.3 burrow systems per hectare, colonial burrow soil contained an amount of inorganic N equal to 21% and 30% of plant-available N in surface soil and subsurface soil, respectively, in an area that represented only 0.35% of the landscape. Our study indicates that burrowing and deposition of waste results in a highly active subsurface layer in which C and N dynamics function much like surface soil when soil moisture is not limiting. (C) Published by Elsevier Ltd. C1 [Clark, Kenneth L.] US Forest Serv, Silas Little Expt Forest, USDA, New Lisbon, NJ 08064 USA. [Branch, Lyn C.] Univ Florida, Dept Wildlife Ecol & Conservat, Gainesville, FL 32611 USA. [Hierro, Jose L.; Villarreal, Diego] Univ Nacl La Pampa UNLPam, Fac Ciencias Exactas & Nat, RA-6300 Santa Rosa, La Pampa, Argentina. [Hierro, Jose L.] CONICET UNLPam, Inst Ciencias Tierra & Ambientales La Pampa INCIT, Mendoza 109, RA-6300 Santa Rosa, La Pampa, Argentina. RP Clark, KL (reprint author), US Forest Serv, Silas Little Expt Forest, USDA, New Lisbon, NJ 08064 USA. EM kennethclark@fs.fed.us NR 48 TC 0 Z9 0 U1 11 U2 11 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD DEC PY 2016 VL 103 BP 253 EP 261 DI 10.1016/j.soilbio.2016.08.027 PG 9 WC Soil Science SC Agriculture GA ED3VA UT WOS:000388775400025 ER PT J AU Burns, KN Bokulich, NA Cantu, D Greenhut, RF Kluepfel, DA O'Geen, AT Strauss, SL Steenwerth, KL AF Burns, Kayla N. Bokulich, Nicholas A. Cantu, Dario Greenhut, Rachel F. Kluepfel, Daniel A. O'Geen, A. Toby Strauss, Sarah L. Steenwerth, Kerri L. TI Vineyard soil bacterial diversity and composition revealed by 16S rRNA genes: Differentiation by vineyard management SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE 16S rDNA; Plant-microbe interactions; Soil organic matter; Adaptive radiation; Firmicutes; Actinobacteria ID MICROBIAL COMMUNITY COMPOSITION; ELEMENTAL ORGANIC-ANALYSIS; SPINACH WILT DISEASE; COVER CROPS; MICROBIOLOGICAL FUNCTION; AGRICULTURAL MANAGEMENT; NITROGEN DON; CARBON DOC; TILLAGE; QUALITY AB Little is known about the hierarchical effects of management practices, soil attributes and location factors on structure of vineyard soil microbiota. A hierarchical effect occurs when the specific influence of an experimental factor (e.g. cover crop type, compost application) on soil-borne bacterial communities is greater within a subset composing the larger set but not across the entire set (e.g. bacterial communities only respond to a management practice within a subset of soil types but not across the entire set composed of all soil types). To address this concept, we measured differences in soil bacterial and archaeal diversity in wine-grape vineyard soils throughout Napa Valley, California. We describe how vineyard management practices influence soil resources, which in turn determine shifts in soil-borne bacterial communities. Soil bacterial communities were structured with respect to management practices, specifically cover crop presence and cover crop mix, tillage, and agricultural system designation, i.e. conventional, organic and biodynamic production systems. Distinctions with respect to management were associated with differences in pH and soil resource pools: total carbon and total nitrogen of the <53 and 53-250 pm particulate organic matter fractions, and potentially mineralizable nitrogen. Findings in this study suggest management practices in vineyard production systems directly influence soil microbial community structure, as mediated by shifts in soil resource pools. However, hierarchical effects occur, in which beta-diversity is more strongly affected by specific management practices only within certain soil types, tillage or no-till soils or winegrowing region. This work allows for subsequent assessments of interrelationships of vineyard management, microbial biodiversity and their combined influence on soil quality, vine health, and berry quality. Published by Elsevier Ltd. C1 [Burns, Kayla N.; Bokulich, Nicholas A.; Cantu, Dario] Univ Calif Davis, Dept Viticulture & Enol, One Shields Ave, Davis, CA 95616 USA. [Bokulich, Nicholas A.] Univ Calif Davis, Foods Hlth Inst, One Shields Ave, Davis, CA 95616 USA. [Bokulich, Nicholas A.] Univ Calif Davis, Dept Food Sci & Technol, One Shields Ave, Davis, CA 95616 USA. [Greenhut, Rachel F.; Kluepfel, Daniel A.; Strauss, Sarah L.; Steenwerth, Kerri L.] Univ Calif Davis, USDA ARS, Crops Pathol & Genet Res Unit, One Shields Ave, Davis, CA 95616 USA. [O'Geen, A. Toby] Univ Calif Davis, Dept Land Air & Water Resources, One Shields Ave, Davis, CA 95616 USA. RP Steenwerth, KL (reprint author), Univ Calif Davis, USDA ARS, Crops Pathol & Genet Res Unit, One Shields Ave, Davis, CA 95616 USA. EM knburns@ucdavis.edu; nbokulich@ucdavis.edu; dacantu@ucdavis.edu; rachel.greenhut@ars.usda.gov; daniel.kluepfel@ars.usda.gov; atogeen@ucdavis.edu; sarah.strauss@ars.usda.gov; kerri.steenwerth@ars.usda.gov RI Cantu, Dario /E-7658-2010 OI Cantu, Dario /0000-0002-4858-1508 FU CDFA Specialty Crops Block Grant [SCB09042]; UC Davis; American Vineyard Foundation [2011-852]; USDA-ARS Sustainable Viticulture Production Systems [2032-21220-006-00, NP305] FX We thank Dr. J. Deng, Dr. E. Gutierrez-Rodriguez, Dr. K. Kalanetra, C. Ko, M. Lee, C. Masarweh, Dr. A. McClean, Dr. D. Mills, M. Ryazantseva, M. Saal, J. Smith, and O. Yu for technical support, and the participating growers for participating in interviews and allowing access to their vineyards. Support for this research was provided by the CDFA Specialty Crops Block Grant #SCB09042, Henry A. Jastro Graduate Research Award from UC Davis, Andre Tchelistcheff & Dr. Richard Peterson Scholarship from the American Vineyard Foundation (#2011-852), and USDA-ARS Sustainable Viticulture Production Systems (Project 2032-21220-006-00, NP305 Crop Production). NR 98 TC 0 Z9 0 U1 31 U2 31 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD DEC PY 2016 VL 103 BP 337 EP 348 DI 10.1016/j.soilbio.2016.09.007 PG 12 WC Soil Science SC Agriculture GA ED3VA UT WOS:000388775400034 ER PT J AU DeJesus, E Costa-Hurtado, M Smith, D Lee, DH Spackman, E Kapczynski, DR Torchetti, MK Killian, ML Suarez, DL Swayne, DE Pantin-Jackwood, MJ AF DeJesus, Eric Costa-Hurtado, Mar Smith, Diane Lee, Dong-Hun Spackman, Erica Kapczynski, Darrell R. Torchetti, Mia Kim Killian, Mary L. Suarez, David L. Swayne, David E. Pantin-Jackwood, Mary J. TI Changes in adaptation of H5N2 highly pathogenic avian influenza H5 Glade 2.3.4.4 viruses in chickens and mallards SO VIROLOGY LA English DT Article DE H5N2; H5N8; H5N1; Highly pathogenic avian influenza virus; Chickens; Mallards; Infectivity; Pathogenicity; Transmission; Adaptation ID MULTIPLE SEQUENCE ALIGNMENT; MIGRATORY BIRDS; PACIFIC FLYWAY; EURASIAN H5N8; UNITED-STATES; A H5N2; POULTRY; DUCKS; PATHOBIOLOGY; INFECTION AB H5N2 highly pathogenic avian influenza (HPAI) viruses caused a severe poultry outbreak in the United States (U.S.) during 2015. In order to examine changes in adaptation of this viral lineage, the infectivity, pathogenicity and transmission of poultry H5N2 viruses were investigated in chickens and mallards in comparison to the wild duck 2014 U.S. index H5N2 virus. The four poultry isolates examined had a lower mean bird infectious dose than the index virus but still transmitted poorly to direct contacts. In mallards, two of the H5N2 poultry isolates had similar high infectivity and transmissibility as the index H5N2 virus, the H5N8 U.S. index virus, and a 2005 H5N1 Glade 2.2 virus. Mortality occurred with the H5N1 virus and, interestingly, with one of two poultry H5N2 isolates. Increased virus adaptation to chickens was observed with the poultry H5N2 viruses; however these viruses retained high adaptation to mallards but pathogenicity was differently affected. Published by Elsevier Inc. C1 [DeJesus, Eric; Costa-Hurtado, Mar; Smith, Diane; Lee, Dong-Hun; Spackman, Erica; Kapczynski, Darrell R.; Suarez, David L.; Swayne, David E.; Pantin-Jackwood, Mary J.] ARS, Exot & Emerging Avian Viral Dis Res Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr,USDA, Athens, GA 30605 USA. [Torchetti, Mia Kim; Killian, Mary L.] USDA, Natl Vet Serv Labs, Vet Serv, Ames, IA USA. [Costa-Hurtado, Mar] Univ Montreal, Fac Vet Med, Res Grp Infect Dis Prod Anim, St Hyacinthe, PQ, Canada. [Costa-Hurtado, Mar] Univ Montreal, Fac Vet Med, Swine & Poultry Infect Dis Res Ctr, St Hyacinthe, PQ, Canada. RP Pantin-Jackwood, MJ (reprint author), ARS, Exot & Emerging Avian Viral Dis Res Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr,USDA, Athens, GA 30605 USA. EM eric.dejesus@ars.usda.gov; mar.costa-hurtado@umontreal.ca; diane.smith@ars.usda.gov; donghun.lee@ars.usda.gov; erica.spackman@ars.usda.gov; darrell.kapczynski@ars.usda.gov; mia.kim.torchetti@aphis.usda.gov; mary.l.killian@aphis.usda.gov; david.suarez@ars.usda.gov; david.swayne@ars.usda.gov; mary.pantin-jackwood@ars.usda.gov FU USDA/ARS CRIS Project [6612-32000-063-00D]; CRIP (Center of Research in Influenza Pathogenesis) an NIAID - Center of Excellence in Influenza Research and Surveillance (CEIRS) [HHSN272201400008C] FX This research was supported by the USDA/ARS CRIS Project 6612-32000-063-00D and by CRIP (Center of Research in Influenza Pathogenesis) an NIAID funded Center of Excellence in Influenza Research and Surveillance (CEIRS, contract HHSN272201400008C). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the USDA or NIH. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The authors appreciate the technical assistance provided by Kira Moresco and Nicolai Lee, and the animal care provided by Keith Crawford, Gerald Damron, and Roger Brock in conducting these studies. USDA is an equal opportunity provider and employer. NR 37 TC 1 Z9 1 U1 5 U2 5 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD DEC PY 2016 VL 499 BP 52 EP 64 DI 10.1016/j.virol.2016.08.036 PG 13 WC Virology SC Virology GA EC3YI UT WOS:000388062600006 PM 27632565 ER PT J AU Segundo, FDS Montiel, NA Sturza, DF Perez-Martin, E Hickman, D Ramirez-Medina, E Grubman, MJ de los Santosa, T AF Segundo, Fayna Diaz-San Montiel, Nestor A. Sturza, Diego F. Perez-Martin, Eva Hickman, Danielle Ramirez-Medina, Elizabeth Grubman, Marvin J. de los Santosa, Teresa TI Combination of Adt-O1Manisa and Ad5-boIFN lambda 3 induces early protective immunity against foot-and-mouth disease in cattle SO VIROLOGY LA English DT Article DE FMDV; Ad5-boIFN lambda 3; Foot-and-mouth disease; Early protection adjuvant effect; Cattle; Ad5-vector vaccine ID HEPATITIS-C VIRUS; DELTA T-CELLS; INNATE LYMPHOID-CELLS; MOLECULAR-CLONING; SUBUNIT VACCINE; IFN-LAMBDA; ACTIVATION ANTIGEN-B7; ANTIBODY-RESPONSE; RAPID PROTECTION; INTERFERON-ALPHA AB Foot-and-mouth-disease (FMD) remains the most infectious livestock disease worldwide. Although commercially available inactivated or adenovirus-vectored-vaccines (Ad5-FMD) are effective, they require 5-7 days to induce protection. Therefore, new control strategies that stimulate rapid immune responses are needed. Expression of bovine interferon lambda 3 using the Ad5-vector platform (Ad5-boIFN lambda 3) is able to delay disease in cattle, but clinical signs appear at 9 days after challenge. We hypothesized that combination of Ad5-boIFN lambda 3 and Ad5-FMD could induce immediate and lasting protection against FMD. Cattle were vaccinated with an Ad5-FMD, Ad5-boIFN lambda 3, or the combination of both, followed by challenge at three days post-immunization. All animals treated with Ad5-FMD combined with Ad5-boIFN lambda 3 were fully protected against FMD, despite the absence of systemic neutralizing antibodies or antiviral activity at the time of challenge. Induction of a strong cell-mediated immune response suggested that Ad5-boIFN lambda 3 is able to act as an adjuvant of Ad5-FMD vaccine in cattle. C1 [Segundo, Fayna Diaz-San; Montiel, Nestor A.; Sturza, Diego F.; Perez-Martin, Eva; Hickman, Danielle; Ramirez-Medina, Elizabeth; Grubman, Marvin J.; de los Santosa, Teresa] ARS, Plum Isl Anim Dis Ctr, North Atlantic Area, USDA, Greenport, NY 11944 USA. [Montiel, Nestor A.; Sturza, Diego F.; Perez-Martin, Eva; Ramirez-Medina, Elizabeth] Oak Ridge Inst Sci & Educ, Plum Isl Anim Dis Ctr, Res Participat Program, Oak Ridge, TN 37831 USA. [Segundo, Fayna Diaz-San] Univ Connecticut, Dept Pathobiol & Vet Sci, Storrs, CT 06269 USA. RP de los Santosa, T (reprint author), ARS, Plum Isl Anim Dis Ctr, North Atlantic Area, USDA, Greenport, NY 11944 USA. EM teresa.delossantos@ars.usda.gov FU ARS-CRIS Project [1940-32000-057-00D]; ARS; Science and Technology Directorate of the Department of Homeland Security (DHS) [HSHQDC-11-X-00189, HSHQPM-13-X-00113]; USDA [58-1940-4-003]; University of Connecticut [58-1940-4-003]; Plum Island Animal Disease Center Research Participation Program FX This work was funded by ARS-CRIS Project 1940-32000-057-00D and ARS interagency agreements with the Science and Technology Directorate of the Department of Homeland Security (DHS) (award# HSHQDC-11-X-00189 and HSHQPM-13-X-00113) and Specific Cooperative Agreement #58-1940-4-003 between USDA and University of Connecticut. NAM, DFS and EPM are recipients of a Plum Island Animal Disease Center Research Participation Program fellowship, administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement with the U.S. Department of Energy. We thank DHS for providing Adt. O1M vaccine produced by GenVec Inc. We are grateful to PIADC animal research branch for professional assistance with animal experiments. NR 77 TC 0 Z9 0 U1 4 U2 4 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD DEC PY 2016 VL 499 BP 340 EP 349 DI 10.1016/j.virol.2016.09.027 PG 10 WC Virology SC Virology GA EC3YI UT WOS:000388062600036 ER PT J AU Coopersmith, EJ Cosh, MH Bell, JE Boyles, R AF Coopersmith, Evan J. Cosh, Michael H. Bell, Jesse E. Boyles, Ryan TI Using machine learning to produce near surface soil moisture estimates from deeper in situ records at US Climate Reference Network (USCRN) locations: Analysis and applications to AMSR-E satellite validation SO ADVANCES IN WATER RESOURCES LA English DT Article DE Soil moisture; Remote sensing; Modeling; USCRN; AMSR-E; ECONet ID MODELS; STABILITY; WATER AB Surface soil moisture is a critical parameter for understanding the energy flux at the land atmosphere boundary. Weather modeling, climate prediction, and remote sensing validation are some of the applications for surface soil moisture information. The most common in situ measurement for these purposes are sensors that are installed at depths of approximately 5 cm. There are however, sensor technologies and network designs that do not provide an estimate at this depth. If soil moisture estimates at deeper depths could be extrapolated to the near surface, in situ networks providing estimates at other depths would see their values enhanced. Soil moisture sensors from the U.S. Climate Reference Network (USCRN) were used to generate models of 5 cm soil moisture, with 10 cm soil moisture measurements and antecedent precipitation as inputs, via machine learning techniques. Validation was conducted with the available, in situ, 5 cm resources. It was shown that a 5 cm estimate, which was extrapolated from a 10 cm sensor and antecedent local precipitation, produced a root-mean-squared-error (RMSE) of 0.0215 m(3)/m(3). Next, these machine-learning-generated 5 cm estimates were also compared to AMSR-E estimates at these locations. These results were then compared with the performance of the actual in situ readings against the AMSR-E data. The machine learning estimates at 5 cm produced an RMSE of approximately 0.03 m(3)/m(3) when an optimized gain and offset were applied. This is necessary considering the performance of AMSR-E in locations characterized by high vegetation water contents, which are present across North Carolina. Lastly, the application of this extrapolation technique is applied to the ECONet in North Carolina, which provides a 10 cm depth measurement as its shallowest soil moisture estimate. A raw RMSE of 0.028 m(3)/m(3) was achieved, and with a linear gain and offset applied at each ECONet site, an RMSE of 0.013 m(3)/m(3) was possible. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Coopersmith, Evan J.; Cosh, Michael H.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Bell, Jesse E.] Cooperat Inst Climate & Satellites NC, Asheville, NC USA. [Bell, Jesse E.] NOAA, Natl Ctr Environm Informat, Asheville, NC USA. [Boyles, Ryan] North Carolina State Univ, Southeast Climate Sci Ctr, Raleigh, NC 27695 USA. RP Coopersmith, EJ (reprint author), USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. EM ecooper2@gmail.com OI Coopersmith, Evan/0000-0002-6223-4828 NR 36 TC 0 Z9 0 U1 21 U2 21 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0309-1708 EI 1872-9657 J9 ADV WATER RESOUR JI Adv. Water Resour. PD DEC PY 2016 VL 98 BP 122 EP 131 DI 10.1016/j.advwatres.2016.10.007 PG 10 WC Water Resources SC Water Resources GA ED2IQ UT WOS:000388667600008 ER PT J AU Bobryk, CW Rega-Brodsky, CC Bardhan, S Farina, A He, HS Jose, S AF Bobryk, Christopher W. Rega-Brodsky, Christine C. Bardhan, Sougata Farina, Almo He, Hong S. Jose, Shibu TI A rapid soundscape analysis to quantify conservation benefits of temperate agroforestry systems using low-cost technology SO AGROFORESTRY SYSTEMS LA English DT Article DE Acoustic complexity index (ACI); Conservation; Indicators; Low-cost recorder (LCR); Rapid assessment ID AGRICULTURAL INTENSIFICATION; BIODIVERSITY CONSERVATION; LANDSCAPE; NOISE; ECOLOGY; BIRDS; HYPOTHESIS; HABITAT; SUCCESS; CITY AB Quantifying the impacts of agroforestry systems (AFS) on habitat conservation remains a challenge due to difficulties in accounting for differences in scales, AFS configurations, and inadequate measures of species dynamics within practical time frames. The field of soundscape ecology offers new perspectives and tools to efficiently collect information on species richness and has a potential to be used as a holistic measure for indicating changes in habitat conditions. The objective of this investigation was to conduct a rapid soundscape assessment using low-cost technology and evaluate the efficacy of using sounds as an indicator for monitoring and assessing species richness within AFS. A series of low-cost recorders were placed within two AFS (pecan alley crop and silvopasture) and two control habitats (natural forest and soybean monoculture). The acoustic complexity index (ACI) was used as the metric to quantify the sonic environment. Results indicated a weak, but significant relationship (R-2 = 0.30) between the ACI and overall structural complexity, measured using median heights, across different land-use systems. There was a stronger relationship (R-2 = 0.53) between the ACI metric and soundscape composition, which was defined by overall species richness. This study demonstrated the usefulness of conducting a rapid, low-cost soundscape analysis that quantified the effects of different land-use systems on species richness. This research also helped provide evidence of the significance of AFS as an integrative land-use system with beneficial characteristics that have potential to promote both production and ecological conservation. C1 [Bobryk, Christopher W.] USDA, Agr Res Serv, 269 Agr Engn Bldg, Columbia, MO 65211 USA. [Rega-Brodsky, Christine C.] Univ Missouri, Dept Fisheries & Wildlife, 302 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. [Bardhan, Sougata; He, Hong S.; Jose, Shibu] Univ Missouri, Dept Forestry, Ctr Agroforestry, 203 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. [Farina, Almo] Univ Urbino, Dept Basic Sci & Fdn, Campus Sci Enrico Mattei, I-61029 Urbino, Italy. RP Bobryk, CW (reprint author), USDA, Agr Res Serv, 269 Agr Engn Bldg, Columbia, MO 65211 USA. EM c.w.bobryk@mail.missouri.edu FU College of Agriculture, Food, and Natural Resources at the University of Missouri; Center for Agroforestry at the University of Missouri FX We would like to thank the Dorris D. and Christine M. Brown Fellowship offered by the College of Agriculture, Food, and Natural Resources at the University of Missouri, and The Center for Agroforestry at the University of Missouri for partially funding this project. NR 43 TC 0 Z9 0 U1 21 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-4366 EI 1572-9680 J9 AGROFOREST SYST JI Agrofor. Syst. PD DEC PY 2016 VL 90 IS 6 BP 997 EP 1008 DI 10.1007/s10457-015-9879-6 PG 12 WC Agronomy; Forestry SC Agriculture; Forestry GA EC5MX UT WOS:000388180800005 ER PT J AU Branas, CC Kondo, MC Murphy, SM South, EC Polsky, D MacDonald, JM AF Branas, Charles C. Kondo, Michelle C. Murphy, Sean M. South, Eugenia C. Polsky, Daniel MacDonald, John M. TI Urban Blight Remediation as a Cost-Beneficial Solution to Firearm Violence SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Article ID BINARY RESPONSE DATA; UNITED-STATES; PUBLIC-HEALTH; CRIME; REGRESSION; MODELS; SPACE; ENVIRONMENT; MORTALITY; COUNTRIES AB Objectives. To determine if blight remediation of abandoned buildings and vacant lots can be a cost-beneficial solution to firearm violence in US cities. Methods. We performed quasi-experimental analyses of the impacts and economic returns on investment of urban blight remediation programs involving 5112 abandoned buildings and vacant lots on the occurrence of firearm and nonfirearm violence in Philadelphia, Pennsylvania, from 1999 to 2013. We adjusted before-after percent changes and returns on investment in treated versus control groups for sociodemographic factors. Results. Abandoned building remediation significantly reduced firearm violence -39% (95% confidence interval [CI] = -28%, -50%; P < .05) as did vacant lot remediation (-4.6%; 95% CI = -4.2%, -5.0%; P < .001). Neither program significantly affected nonfirearm violence. Respectively, taxpayer and societal returns on investment for the prevention of firearm violence were $5 and $79 for every dollar spent on abandoned building remediation and $26 and $333 for every dollar spent on vacant lot remediation. Conclusions. Abandoned buildings and vacant lots are blighted structures seen daily by urban residents that may create physical opportunities for violence by sheltering illegal activity and illegal firearms. Urban blight remediation programs can be cost-beneficial strategies that significantly and sustainably reduce firearm violence. C1 [Branas, Charles C.] Univ Penn, Perelman Sch Med, Dept Biostat & Epidemiol, Philadelphia, PA 19104 USA. [Branas, Charles C.] Univ Penn, Leonard Davis Inst Hlth Econ, Perelman Sch Med, Philadelphia, PA 19104 USA. [Kondo, Michelle C.] US Forest Serv, USDA, Northern Res Stn, Philadelphia, PA USA. [Murphy, Sean M.] Washington State Univ, Dept Hlth Policy & Adm, Spokane, WA USA. [South, Eugenia C.] Univ Penn, Dept Emergency Med, Perelman Sch Med, Philadelphia, PA 19104 USA. [Polsky, Daniel] Univ Penn, Leonard Davis Inst Hlth Econ, Wharton Sch Business, Philadelphia, PA 19104 USA. [Polsky, Daniel] Univ Penn, Perelman Sch Med, Philadelphia, PA 19104 USA. [MacDonald, John M.] Univ Penn, Sch Arts & Sci, Dept Criminol, Philadelphia, PA 19104 USA. [MacDonald, John M.] Univ Penn, Leonard Davis Inst Hlth Econ, Sch Arts & Sci, Philadelphia, PA 19104 USA. RP Branas, CC (reprint author), Univ Penn, Perelman Sch Med, Epidemiol, Philadelphia, PA 19104 USA.; Branas, CC (reprint author), Univ Penn, Perelman Sch Med, Penn Urban Hlth Lab, Philadelphia, PA 19104 USA.; Branas, CC (reprint author), Univ Penn, Perelman Sch Med, Penn Injury Sci Ctr, Philadelphia, PA 19104 USA. EM cbranas@upenn.edu FU National Institutes of Health [R01AA020331]; Centers for Disease Control and Prevention [R49CE002474, U01CE002698]; Robert Wood Johnson Foundation FX This study was funded in part by the National Institutes of Health (grant R01AA020331), the Centers for Disease Control and Prevention (grants R49CE002474 and U01CE002698), and the Robert Wood Johnson Foundation. NR 57 TC 3 Z9 3 U1 3 U2 3 PU AMER PUBLIC HEALTH ASSOC INC PI WASHINGTON PA 800 I STREET, NW, WASHINGTON, DC 20001-3710 USA SN 0090-0036 EI 1541-0048 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD DEC PY 2016 VL 106 IS 12 BP 2158 EP 2164 DI 10.2105/AJPH.2016.303434 PG 7 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA EC4IQ UT WOS:000388091700039 PM 27736217 ER PT J AU Afrasiabi, Z Popham, HJR Stanley, D Suresh, D Finley, K Campbell, J Kannan, R Upendran, A AF Afrasiabi, Zahra Popham, Holly J. R. Stanley, David Suresh, Dhananjay Finley, Kristen Campbell, Jonelle Kannan, Raghuraman Upendran, Anandhi TI DIETARY SILVER NANOPARTICLES REDUCE FITNESS IN A BENEFICIAL, BUT NOT PEST, INSECT SPECIES SO ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY LA English DT Article DE Heliothis virescens; Trichoplusia ni; Podisus maculiventris; silver nanoparticles ID DROSOPHILA-MELANOGASTER; TOXICITY; EXPOSURE; HISTORY AB Silver nanoparticles (AgNPs) have antimicrobial and insecticidal properties and they have been considered for their potential use as insecticides. While they do, indeed, kill some insects, two broader issues have not been considered in a critical way. First, reports of insect-lethal AgNPs are often based on simplistic methods that yield nanoparticles of nonuniform shapes and sizes, leaving questions about the precise treatments test insects experienced. Second, we do not know how AgNPs influence beneficial insects. This work addresses these issues. We assessed the influence of AgNPs on life history parameters of two agricultural pest insect species, Heliothis virescens (tobacco budworm) and Trichoplusia ni (cabbage looper) and a beneficial predatory insect species, Podisus maculiventris (spined soldier bug), all of which act in agroecosystems. Rearing the two pest species on standard media amended with AgNPs led to negligible influence on developmental times, pupal weights, and adult emergence, however, they led to retarded development, reductions in adult weight and fecundity, and increased mortality in the predator. These negative effects on the beneficial species, if also true for other beneficial insect species, would have substantial negative implications for continued development of AgNPs for insect pest management programs. (C) 2016 Wiley Periodicals, Inc. C1 [Afrasiabi, Zahra; Finley, Kristen; Campbell, Jonelle] Lincoln Univ, Dept Life & Phys Sci, Jefferson City, MO 65101 USA. [Afrasiabi, Zahra] Soka Univ Amer, Dept Math & Sci, Aliso Viejo, CA USA. [Popham, Holly J. R.; Stanley, David] USDA ARS, Biol Control Insects Res Lab, Columbia, MO 65205 USA. [Suresh, Dhananjay; Kannan, Raghuraman] Univ Missouri, Dept Bioengn, Columbia, MO USA. [Kannan, Raghuraman] Univ Missouri, Dept Radiol, Columbia, MO USA. [Upendran, Anandhi] Univ Missouri, Sch Med, Inst Clin & Translat Sci, Columbia, MO 65212 USA. [Upendran, Anandhi] Univ Missouri, Dept Phys, Columbia, MO 65211 USA. RP Afrasiabi, Z (reprint author), Lincoln Univ, Dept Life & Phys Sci, Jefferson City, MO 65101 USA.; Upendran, A (reprint author), Univ Missouri, Sch Med, Inst Clin & Translat Sci, Columbia, MO 65212 USA. EM afrasigbiz@lincolnu.edu; upendrana@missouri.edu OI Suresh, Dhananjay/0000-0003-3490-5636 FU USDA-NIFA Capacity Building Grant [Afrasiabi-2011-02535] FX Grant sponsor: USDA-NIFA Capacity Building Grant Afrasiabi-2011-02535. NR 23 TC 0 Z9 0 U1 1 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0739-4462 EI 1520-6327 J9 ARCH INSECT BIOCHEM JI Arch. Insect Biochem. Physiol. PD DEC PY 2016 VL 93 IS 4 BP 190 EP 201 DI 10.1002/arch.21351 PG 12 WC Biochemistry & Molecular Biology; Entomology; Physiology SC Biochemistry & Molecular Biology; Entomology; Physiology GA ED1UP UT WOS:000388630700002 PM 27509382 ER PT J AU Elias, CV Power, TG Beck, AE Goodell, LS Johnson, SL Papaioannou, MA Hughes, SO AF Elias, Cindy V. Power, Thomas G. Beck, Ashley E. Goodell, L. Suzanne Johnson, Susan L. Papaioannou, Maria A. Hughes, Sheryl O. TI Depressive Symptoms and Perceptions of Child Difficulty Are Associated with Less Responsive Feeding Behaviors in an Observational Study of Low-Income Mothers SO CHILDHOOD OBESITY LA English DT Article ID WEIGHT STATUS; MATERNAL DEPRESSION; PARENTING STRESS; YOUNG-CHILDREN; STYLES; OBESITY; SAMPLE; STRATEGIES; DISORDERS; FAMILIES AB Background: Maternal depressive symptoms and perceptions of child difficulty are associated with negative effects on general development and cognitive functioning in children. The study examined associations between maternal depressive symptoms, perceptions of child difficulty, and maternal feeding behaviors in a population at elevated risk for childhood obesity. Methods: Participants were 138 low-income black and Hispanic mothers and their children (ages 3-5) participating in an observational study of mealtimes among Head Start families. Three dinnertime observations were conducted over 2 weeks on each family and audio/videotaped for coding. Coding included eating influence attempts and other food-and nonfood-related interactions exhibited by the mother during dinner. Mothers completed questionnaires on depressive symptoms and perceptions of child difficulty. Linear regressions were conducted, examining associations between maternal depressive symptoms, perceptions of child difficulty, and coded parent feeding behaviors. Results: Mothers reporting higher levels of depressive symptoms used more verbal pressure to get their child to eat during meals, were more likely to discourage child independence, and less likely to enforce table manners. Mothers reporting higher perceptions of child difficulty were less likely to have nonfood-related discussions during meals and to try to get the child to eat a different food. Conclusions: This study is one of the first to investigate associations between maternal depression, perceptions of child difficulty, and mother's feeding behaviors during meals using observational methodology. These results may help researchers identify specific parental characteristics and feeding practices on which to intervene when developing tailored intervention programs for reducing childhood obesity. C1 [Elias, Cindy V.; Papaioannou, Maria A.; Hughes, Sheryl O.] USDA, Dept Pediat, ARS Childrens Nutr Res Ctr, Baylor Coll Med, 1100 Bates, Houston, TX 77030 USA. [Power, Thomas G.; Beck, Ashley E.] Washington State Univ, Dept Human Dev, Pullman, WA USA. [Goodell, L. Suzanne] North Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC USA. [Johnson, Susan L.] Univ Colorado Denver, Dept Pediat, Aurora, CO USA. RP Hughes, SO (reprint author), USDA, Dept Pediat, ARS Childrens Nutr Res Ctr, Baylor Coll Med, 1100 Bates, Houston, TX 77030 USA. EM shughes@bcm.edu FU US Department of Agriculture [USDA 2006-55215-16695, USDA 2011-68001-30009]; USDA/ARS [58-3092-5-001] FX This research was supported by funds from the US Department of Agriculture (USDA 2006-55215-16695 and USDA 2011-68001-30009). This work is a publication of the US Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas, and has been funded, in part, with federal funds from the USDA/ARS under Cooperative Agreement No. 58-3092-5-001. The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement from the U.S. government. The authors have no financial relationships relevant to this article to disclose. NR 42 TC 0 Z9 0 U1 8 U2 8 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2153-2168 EI 2153-2176 J9 CHILD OBES JI Child Obes. PD DEC PY 2016 VL 12 IS 6 BP 418 EP 425 DI 10.1089/chi.2016.0125 PG 8 WC Pediatrics SC Pediatrics GA EC5RP UT WOS:000388194200002 PM 27662419 ER PT J AU Gibson, AL Espeland, EK Wagner, V Nelson, CR AF Gibson, Alexis L. Espeland, Erin K. Wagner, Viktoria Nelson, Cara R. TI Can local adaptation research in plants inform selection of native plant materials? An analysis of experimental methodologies SO EVOLUTIONARY APPLICATIONS LA English DT Article DE ecological experiments; experimental design; experimental methodology; lifetime fitness; local adaptation; plants ID TRANSGENERATIONAL PLASTICITY; POPULATION DIFFERENTIATION; CHAMAECRISTA-FASCICULATA; ECOLOGICAL RESTORATION; CONCEPTUAL ISSUES; GENETIC-VARIATION; CLIMATE-CHANGE; COMMON-GARDEN; SEED; HERB AB Local adaptation is used as a criterion to select plant materials that will display high fitness in new environments. A large body of research has explored local adaptation in plants, however, to what extent findings can inform management decisions has not been formally evaluated. We assessed local adaptation literature for six key experimental methodologies that have the greatest effect on the application of research to selecting plant materials for natural resource management: experimental environment, response variables, maternal effects, intraspecific variation, selective agents, and spatial and temporal variability. We found that less than half of experiments used reciprocal transplants or natural field conditions, which are both informative for revegetation and restoration. Population growth rate was rarely (5%) assessed, and most studies measured only single generations (96%) and ran for less than a year. Emergence and establishment are limiting factors in successful revegetation and restoration, but the majority of studies measured later life-history stages (66%). Additionally, most studies included limited replication at the population and habitat levels and tested response to single abiotic selective factors (66%). Local adaptation research should be cautiously applied to management; future research could use alternative methodologies to allow managers to directly apply findings. C1 [Gibson, Alexis L.; Wagner, Viktoria; Nelson, Cara R.] Univ Montana, Coll Forestry & Conservat, 32 Campus Dr, Missoula, MT 59803 USA. [Espeland, Erin K.] USDA ARS NPARL, Pest Management Res Unit, Sidney, MT USA. [Wagner, Viktoria] Masaryk Univ, Dept Bot & Zool, Kotlarska 2, CZ-61137 Brno, Czech Republic. RP Gibson, AL (reprint author), Univ Montana, Coll Forestry & Conservat, 32 Campus Dr, Missoula, MT 59803 USA. EM alexis.gibson@umontana.edu FU USDA Forest Service NFN program [09-CS-11015600-031]; National Science Foundation EPSCoR program at the University of Montana [EPS-1101342]; Alexander von Humboldt Foundation FX This research was supported by agreement #09-CS-11015600-031 from the USDA Forest Service NFN program and the National Science Foundation EPSCoR program (#EPS-1101342) at the University of Montana. VW was supported by a postdoctoral fellowship by the Alexander von Humboldt Foundation. Thanks to Drs. Ray Callaway, Lila Fishman, Elizabeth Crone, Dean Pearson and Solomon Dobrowski for feedback. NR 81 TC 0 Z9 0 U1 24 U2 24 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1752-4571 J9 EVOL APPL JI Evol. Appl. PD DEC PY 2016 VL 9 IS 10 BP 1219 EP 1228 DI 10.1111/eva.12379 PG 10 WC Evolutionary Biology SC Evolutionary Biology GA EC0BX UT WOS:000387763700002 PM 27877201 ER PT J AU Moreno, JP Vaughan, E Hernandez, D Cameron, RT Foreyt, JP Johnston, CA AF Moreno, Jennette P. Vaughan, Elizabeth Hernandez, Daphne Cameron, Ryan T. Foreyt, John P. Johnston, Craig A. TI The Impact of Acculturation Level on Weight Status and Weight Outcomes in Hispanic Children SO JOURNAL OF RACIAL AND ETHNIC HEALTH DISPARITIES LA English DT Article DE Acculturation; Obesity; Adolescents; Hispanic; School-based program ID MEXICAN-AMERICAN CHILDREN; BODY-MASS INDEX; UNITED-STATES; PHYSICAL-ACTIVITY; FOOD INSECURITY; FUTURE-RESEARCH; OBESITY; OVERWEIGHT; ADOLESCENTS; CHILDHOOD AB Background Previous studies revealed that higher levels of acculturation are related to obesity in Hispanic adults. Conflicting findings exist regarding this relationship in children, and little is known about the impact of acculturation on children's success in pediatric weight management programs. The purposes of the study were to (1) examine the relationship between acculturation and overweight/obese weight status and (2) determine the impact of acculturation on the changes in weight status among overweight/obese children 12 and 24 months after having participated in a weight management intervention. Methods This is a secondary analysis of aggregated data from three randomized control trials that occurred between 2005 and 2009. Height, weight, and level of acculturation using the Child Short Scale for Hispanics (C-SASH) were measured in a sample of Hispanic children (n = 559). Logistic regression models were used to study phase 1 (n = 559) and phase 2 (n = 142), controlling for child and family characteristics. Results Children reporting high levels of acculturation had a 52 % lower odds of being overweight or obese. Among overweight/obese children who participated in the intervention, high levels of acculturation demonstrated greater reductions in standardized body mass index (zBMI) at 24 months. Conclusions The results of this study indicate a need to tailor weight management programs for Hispanic children who have lower levels of acculturation. C1 [Moreno, Jennette P.; Cameron, Ryan T.] Baylor Coll Med, USDA, ARS, Childrens Nutr Res Ctr,Dept Pediat Nutr, Houston, TX 77030 USA. [Vaughan, Elizabeth; Foreyt, John P.] Baylor Coll Med, Dept Med, Houston, TX 77030 USA. [Hernandez, Daphne; Johnston, Craig A.] Univ Houston, Dept Hlth & Human Performance, Houston, TX USA. [Hernandez, Daphne; Johnston, Craig A.] Univ Houston, Texas Obes Res Ctr, Houston, TX USA. RP Moreno, JP (reprint author), Baylor Coll Med, USDA, ARS, Childrens Nutr Res Ctr,Dept Pediat Nutr, 1100 Bates Ave, Houston, TX 77030 USA. EM palcic@bcm.edu NR 46 TC 1 Z9 1 U1 8 U2 8 PU SPRINGER INTERNATIONAL PUBLISHING AG PI CHAM PA GEWERBESTRASSE 11, CHAM, CH-6330, SWITZERLAND SN 2197-3792 EI 2196-8837 J9 J RACIAL ETHN HEALTH JI J. Racial Ethn. Health Disparities PD DEC PY 2016 VL 3 IS 4 BP 582 EP 589 DI 10.1007/s40615-015-0177-9 PG 8 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA EC5TN UT WOS:000388199400004 PM 27294753 ER PT J AU Hoberg, EP Makarikov, AA Tkach, VV Meagher, S Nims, TN Eckerlin, RP Galbreath, KE AF Hoberg, E. P. Makarikov, A. A. Tkach, V. V. Meagher, S. Nims, T. N. Eckerlin, R. P. Galbreath, K. E. TI Insights on the host associations and geographic distribution of Hymenolepis folkertsi (Cestoda: Hymenolepididae) among rodents across temperate latitudes of North America SO PARASITOLOGY RESEARCH LA English DT Article DE Hymenolepis folkertsi; North America; Hymenolepidid tapeworms; Diversity; Faunal assembly ID AROSTRILEPIS EUCESTODA HYMENOLEPIDIDAE; CRICETIDAE ARVICOLINAE; PEROMYSCUS-MANICULATUS; PARASITE DIVERSITY; HELMINTH-PARASITES; CLIMATE-CHANGE; DEER MOUSE; N. SP; FLORIDA; MICE AB Synoptic data and an understanding of helminth parasite diversity among diverse rodent assemblages across temperate latitudes of North America remain remarkably incomplete. Renewed attention to comprehensive survey and inventory to establish the structure of biodiverse faunas is essential in providing indicators and proxies for identifying the outcomes of accelerating change linked to climate warming and anthropogenic forcing. Subsequent to the description of Hymenolepis folkertsi in the oldfield mouse, Peromyscus polionotus, additional specimens of hymenolepidids were collected or discovered in archived museum repositories from multiple species of deer mice (Peromyscus maniculatus, Peromyscus leucopus), the golden mouse (Ochrotomys nuttalli), chipmunks (Tamias striatus, Tamias amoenus), the 13-lined ground squirrel (Ictidomys tridecemlineatus), and tree squirrels (Sciurus carolinensis, Sciurus niger) from disjunct localities in the USA spanning southern Georgia, Virginia, Pennsylvania, Connecticut, the Upper Peninsula of Michigan, Wisconsin, and central Idaho. Specimens were largely consistent morphologically with the original description of H. folkertsi. Initial DNA sequence data, from a portion of the mitochondrial NADH dehydrogenase subunit 1, demonstrated intraspecific variation among three apparently geographically isolated populations attributed to H. folkertsi (uncorrected genetic distances of 2.7 % (Idaho and Michigan), 2.4 % (Virginia + Pennsylvania and Michigan), and 1.89 % (VA + PA and ID). Geography rather than host association explains the distribution and occurrence of H. folkertsi, and host colonization among deer mice, chipmunks, and other sciurids within regional sites is indicated. Genetic divergence revealed across localities for H. folkertsi suggests historically isolated populations, consistent with extended evolutionary and biogeographic trajectories among hymenolepidids and species of Peromyscus and Tamias in North America. Field inventory, that revealed these parasite populations, substantially alters our understanding of the distribution of diversity and provides insights about the nature of the complex relationships that serve to determine cestode faunas in rodents. C1 [Hoberg, E. P.] ARS, Anim Parasit Dis Lab, USDA, Bldg 1180 BARC East,10300,Baltimore Ave, Beltsville, MD 20705 USA. [Makarikov, A. A.] Russian Acad Sci, Inst Systemat & Ecol Anim, Siberian Branch, Frunze Str 11, Novosibirsk 630091, Russia. [Tkach, V. V.] Univ North Dakota, Dept Biol, 10 Cornell St, Grand Forks, ND 58202 USA. [Meagher, S.] Western Illinois Univ, Dept Biol Sci, Macomb, IL 61455 USA. [Nims, T. N.] Georgia State Univ, Dept Sci, Perimeter Coll, 239 Cedar Lane, Covington, GA 30014 USA. [Eckerlin, R. P.] Northern Virginia Community Coll, Dept Mat Sci & Engn, Annandale, VA 22003 USA. [Galbreath, K. E.] Northern Michigan Univ, Dept Biol, 1401 Presque Isle Ave, Marquette, MI 49855 USA. RP Hoberg, EP (reprint author), ARS, Anim Parasit Dis Lab, USDA, Bldg 1180 BARC East,10300,Baltimore Ave, Beltsville, MD 20705 USA. EM eric.hoberg@ars.usda.gov; makarikov@mail.ru; Vasyl.Tkach@email.und.edu; sa-meagher1@wiu.edu; tnims@gsu.edu; reckerlin@nvcc.edu; Kurt.Galbreath@gmail.com RI Makarikov, Arseny/I-5516-2013 OI Makarikov, Arseny/0000-0002-4389-8397 FU Russian Fund for Fundamental Research [14-04-00871-a]; Scott Holman Research Fund; National Science Foundation (NSF); Division of Environmental Biology, NSF [DEB 0196095, 0415668, 1258010, 1256943] FX We acknowledge Oscar Pung for insights about the ecology and distribution of P. polionotus and records for helminth parasites in southeastern cricetids. Kurt Vandegrift kindly provided specimens of cestodes from Pennsylvania. We thank Sara Brant, Collections Manager, Division of Parasitology, Museum of Southwestern Biology, University of New Mexico for assistance in assembling some cestode specimens for the current study. We are grateful to Mariel Campbell, Collections Manager, Division of Genomic Resources, MSB who facilitated access to specimens and collection records from the Beringian Coevolution Project, including synoptic data for hosts and parasites assembled in the Arctos system. Some cestode specimens had been archived in the former US National Parasite Collection now a component of the National Museum of Natural History, Smithsonian Institution. AAM was supported in part by the Russian Fund for Fundamental Research (Project No. 14-04-00871-a). KEG received support from Scott Holman Research Fund administered through Northern Michigan University. We acknowledge support of the National Science Foundation (NSF) for the development of the parasitological collections of the Museum of Southwestern Biology and the Robert and Virginia Rausch Helminthological Collections, as a vital resource for helminthology and biogeography through a grant to J.A. Cook, G. Jarrell, S. Loker, and EPH from the Division of Biological Infrastructure, Biological Research Collections (BRC 1057383). Further, this research represents a continuing contribution of the Beringian Coevolution Project and the Integrated Inventories of Biomes of the Arctic through grants from the Division of Environmental Biology, NSF to JAC, EPH, and KEG (DEB 0196095, 0415668, 1258010, and 1256943). NR 51 TC 0 Z9 0 U1 9 U2 9 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 EI 1432-1955 J9 PARASITOL RES JI Parasitol. Res. PD DEC PY 2016 VL 115 IS 12 BP 4627 EP 4638 DI 10.1007/s00436-016-5255-3 PG 12 WC Parasitology SC Parasitology GA EC5QP UT WOS:000388191400020 PM 27630100 ER PT J AU Li, HY Smigocki, AC AF Li, Haiyan Smigocki, Ann C. TI Wound induced Beta vulgaris polygalacturonase-inhibiting protein genes encode a longer leucine-rich repeat domain and inhibit fungal polygalacturonases SO PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY LA English DT Article DE Sugar beet; PGIP; LRR; Wound response; Disease resistance ID AMINO-ACID SUBSTITUTION; PHASEOLUS-VULGARIS; PLANT DEFENSE; PGIP GENES; DIFFERENTIAL EXPRESSION; REVEALS; INFECTION; PURIFICATION; EVOLUTION; FAMILY AB Polygalacturonase-inhibiting proteins (PGIPs) are leucine-rich repeat (LRR) proteins involved in plant defense. Three sugar beet (Beta vulgaris L.) PGIP genes, BvPGIP1, BvPGIP2 and BvPGIP3, were isolated from breeding line F1016 and BvPGIP2 and BvPGIP3 from F1010. Full-length cDNA sequences of the three BvPGIP genes encoded a 382 or 384 amino acid peptide. They shared closest similarity with five PGIP-like sequences found in the sugar beet reference genome (RefBeet 1.2.1) as well as Bv(FC607)PGIP1 and Bv(FC607)PGIP2 from sugar beet breeding line FC607. The predicted structure of BvPGIP1, 2 and 3 peptides was that of Phaseolus vulgaris PvPGIP2 with the exception that the sugar beet peptides were about 40 amino acids longer and encoded an additional LRR repeat. RT-PCR and qPCR analyses demonstrated BvPGIP gene expression in leaves, petioles, hypocotyls and highest expression in roots. Leaf and root BvPGIP transcripts increased in response to insect feeding and mechanical wounding. An agarose radial diffusion assay showed that BvPGIP protein extracts inhibited polygalacturonases (PGs) of Rhizoctonia solani, Fusarium oxysporum f. sp. gladioli and F. oxysporum f. sp. betae with the exception that PGIP extracts from line F1010 did not inhibit R. solani PGs. These findings identify sugar beet PGIP genes that encode LRR domains that are longer than reported for most other species. In addition, the wound up-regulated BvPGIP gene expression and inhibition of fungal PGs by PGIP extracts suggest that like other plant PGIPs, the sugar beet genes may play an active role in defense responses. Published by Elsevier Ltd. C1 [Li, Haiyan; Smigocki, Ann C.] USDA ARS, Mol Plant Pathol Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Smigocki, AC (reprint author), USDA ARS, Mol Plant Pathol Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM ann.smigocki@ars.usda.gov FU U.S. Department of Agriculture [58-1245-4-002N]; Beet Sugar Development Foundation [58-1245-4-002N] FX This project is in part supported by a Cooperative Agreement No. 58-1245-4-002N between the U.S. Department of Agriculture and the Beet Sugar Development Foundation. NR 48 TC 0 Z9 0 U1 6 U2 6 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0885-5765 J9 PHYSIOL MOL PLANT P JI Physiol. Mol. Plant Pathol. PD DEC PY 2016 VL 96 BP 8 EP 18 DI 10.1016/j.pmpp.2016.06.004 PG 11 WC Plant Sciences SC Plant Sciences GA EC8SW UT WOS:000388414000002 ER PT J AU Anapalli, SS Pettigrew, WT Reddy, KN Ma, LW Fisher, DK Sui, RX AF Anapalli, Saseendran S. Pettigrew, William T. Reddy, Krishna N. Ma, Liwang Fisher, Daniel K. Sui, Ruixiu TI Climate-Optimized Planting Windows for Cotton in the Lower Mississippi Delta Region SO AGRONOMY-BASEL LA English DT Article DE agricultural system model; planting window; Mississippi Delta; irrigation; rainfed cropping systems ID LINT YIELD PRODUCTION; IRRIGATION REGIMES; SOIL-WATER; GROWTH; IMPACT; MODEL; DATES; SIMULATION; SYSTEM; RZWQM AB Unique, variable summer climate of the lower Mississippi (MS) Delta region poses a critical challenge to cotton producers in deciding when to plant for optimized production. Traditional 2-4 year agronomic field trials conducted in this area fail to capture the effects of long-term climate variabilities in the location for developing reliable planting windows for producers. Our objective was to integrate a four-year planting-date field experiment conducted at Stoneville, MS during 2005-2008 with long-term climate data in an agricultural system model and develop optimum planting windows for cotton under both irrigated and rainfed conditions. Weather data collected at this location from 1960 to 2015 and the CSM-CROPGRO-Cotton v4.6 model within the Root Zone Water Quality Model (RZWQM2) were used. The cotton model was able to simulate both the variable planting date and variable water regimes reasonably well: relative errors of seed cotton yield, aboveground biomass, and leaf area index (LAI) were 14%, 12%, and 21% under rainfed conditions and 8%, 16%, and 15% under irrigated conditions, respectively. Planting windows under both rainfed and irrigated conditions extended from mid-March to mid-June: windows from mid-March to the last week of May under rainfed conditions, and from the last week of April to the end of May under irrigated conditions were better suited for optimum yield returns. Within these windows, rainfed cotton tends to lose yield from later plantings, but irrigated cotton benefits; however, irrigation requirements increase as the planting windows advance in time. Irrigated cotton produced about 1000 kg.ha(-1) seed cotton more than rainfed cotton, with irrigation water requirements averaging 15 cm per season. Under rainfed conditions, there is a 5%, 14%, and 27% chance that the seed cotton production is below 1000, 1500, and 2000 kg.ha(-1), respectively. Information developed in this paper can help MS farmers in decision support for cotton planting. C1 [Anapalli, Saseendran S.; Pettigrew, William T.; Reddy, Krishna N.; Fisher, Daniel K.; Sui, Ruixiu] USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. [Ma, Liwang] USDA ARS, Agr Syst Res Unit, Ft Collins, CO 80525 USA. RP Anapalli, SS (reprint author), USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. EM saseendran.anapalli@ars.usda.gov; william.pettigrew@ars.usda.gov; krishna.reddy@ars.usda.gov; liwang.ma@ars.usda.gov OI Anapalli, Saseendran/0000-0001-5238-1894 NR 43 TC 0 Z9 0 U1 1 U2 1 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2073-4395 J9 AGRONOMY-BASEL JI Agronomy-Basel PD DEC PY 2016 VL 6 IS 4 AR 46 DI 10.3390/agronomy6040046 PG 15 WC Agronomy SC Agriculture GA EC2KB UT WOS:000387942200003 ER PT J AU McMaster, GS Edmunds, DA Jones, SM Johnson, JJ Vigil, MF AF McMaster, Gregory S. Edmunds, Debora A. Jones, Sally M. Johnson, Jerry J. Vigil, Merle F. TI Simulating the Probability of Grain Sorghum Maturity before the First Frost in Northeastern Colorado SO AGRONOMY-BASEL LA English DT Article DE Sorghum bicolor; phenology; physiological maturity; simulation model ID CROP PHENOLOGICAL RESPONSES; WINTER-WHEAT; VEGETATIVE DEVELOPMENT; WATER-STRESS; YIELD; GROWTH; NITROGEN; TILLAGE; PROGRAM; PLAINS AB Expanding grain sorghum [Sorghum bicolor (L.) Moench] production northward from southeastern Colorado is thought to be limited by shorter growing seasons due to lower temperatures and earlier frost dates. This study used a simulation model for predicting crop phenology (PhenologyMMS) to estimate the probability of reaching physiological maturity before the first fall frost for a variety of agronomic practices in northeastern Colorado. Physiological maturity for seven planting dates (1 May to 12 June), four seedbed moisture conditions affecting seedling emergence (from Optimum to Planted in Dust), and three maturity classes (Early, Medium, and Late) were simulated using historical weather data from nine locations for both irrigated and dryland phenological parameters. The probability of reaching maturity before the first frost was slightly higher under dryland conditions, decreased as latitude, longitude, and elevation increased, planting date was delayed, and for later maturity classes. The results provide producers with estimates of the reliability of growing grain sorghum in northeastern Colorado. C1 [McMaster, Gregory S.; Edmunds, Debora A.] USDA ARS, Water Management & Syst Res Unit, Ft Collins, CO 80526 USA. [Jones, Sally M.; Johnson, Jerry J.] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Vigil, Merle F.] USDA ARS, Cent Plains Resources Management Res Unit, Akron, OH 80720 USA. RP McMaster, GS (reprint author), USDA ARS, Water Management & Syst Res Unit, Ft Collins, CO 80526 USA. EM greg.mcmaster@ars.usda.gov; debbie.edmunds@ars.usda.gov; sally.jones@colostate.edu; jerry.johnson@colostate.edu; merle.vigil@ars.usda.gov NR 30 TC 0 Z9 0 U1 1 U2 1 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2073-4395 J9 AGRONOMY-BASEL JI Agronomy-Basel PD DEC PY 2016 VL 6 IS 4 AR 44 DI 10.3390/agronomy6040044 PG 17 WC Agronomy SC Agriculture GA EC2KB UT WOS:000387942200001 ER PT J AU Aycock, KI Campbell, RL Lynch, FC Manning, KB Craven, BA AF Aycock, Kenneth I. Campbell, Robert L. Lynch, Frank C. Manning, Keefe B. Craven, Brent A. TI The Importance of Hemorheology and Patient Anatomy on the Hemodynamics in the Inferior Vena Cava SO ANNALS OF BIOMEDICAL ENGINEERING LA English DT Article DE Non-Newtonian blood flow; Patient-specific modeling; Shear-rate histograms; Computational fluid dynamics; Wall shear stress; Blood viscosity; IVC filter ID NEWTONIAN BLOOD-FLOW; DEEP-VEIN THROMBOSIS; NUMERICAL-ANALYSIS; CAROTID-ARTERY; FILTERS; BIFURCATION; SIMULATION; VISCOSITY; RHEOLOGY; MODELS AB Inferior vena cava (IVC) filters have been used for nearly half a century to prevent pulmonary embolism in at-risk patients. However, complications with IVC filters remain common. In this study, we investigate the importance of considering the hemorheological and morphological effects on IVC hemodynamics by simulating Newtonian and non-Newtonian blood flow in three IVC models with varying levels of geometric idealization. Partial occlusion by an IVC filter and a thrombus is also considered. More than 99% of the infrarenal IVC volume is found to contain flow in the nonlinear region of the shear rate-viscosity curve for blood (less than 100 s(-1)) in the unoccluded IVCs. Newtonian simulations performed using the asymptotic viscosity for blood over-predict the non-Newtonian Reynolds numbers by more than a factor of two and under-predict the mean wall shear stress (WSS) by 28-54%. Agreement with the non-Newtonian simulations is better using a characteristic viscosity, but local WSS errors are still large (up to 50%) in the partially occluded cases. Secondary flow patterns in the IVC also depend on the viscosity model and IVC morphological complexity. Non-Newtonian simulations required only a marginal increase in computational expense compared with the Newtonian simulations. We recommend that future studies of IVC hemodynamics consider the effects of hemorheology and IVC morphology when accurate predictions of WSS and secondary flow features are desired. C1 [Aycock, Kenneth I.; Campbell, Robert L.] Penn State Univ, Appl Res Lab, University Pk, PA 16802 USA. [Aycock, Kenneth I.; Manning, Keefe B.] Penn State Univ, Dept Biomed Engn, University Pk, PA 16802 USA. [Campbell, Robert L.] Penn State Univ, Dept Mech & Nucl Engn, University Pk, PA 16802 USA. [Lynch, Frank C.; Manning, Keefe B.] Penn State Hershey Med Ctr, Dept Surg, Hershey, PA USA. [Craven, Brent A.] USDA, Div Appl Mech, Off Sci & Engn Labs, Ctr Devices & Radiol Hlth, Silver Spring, MD USA. RP Manning, KB (reprint author), Penn State Univ, Dept Biomed Engn, University Pk, PA 16802 USA. EM kbm10@psu.edu FU Walker Assistantship program at the Penn State Applied Research Laboratory FX The authors thank Elaheh Rahbar, Daisuke Mori, and James E. Moore Jr. for generously providing the geometry for the patient-averaged IVC model. This research was supported by the Walker Assistantship program at the Penn State Applied Research Laboratory. NR 51 TC 0 Z9 0 U1 4 U2 4 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0090-6964 EI 1573-9686 J9 ANN BIOMED ENG JI Ann. Biomed. Eng. PD DEC PY 2016 VL 44 IS 12 BP 3568 EP 3582 DI 10.1007/s10439-016-1663-x PG 15 WC Engineering, Biomedical SC Engineering GA EC4MB UT WOS:000388103900011 PM 27272211 ER PT J AU Chen, Y Burall, LS Luo, Y Timme, R Melka, D Muruvanda, T Payne, J Wang, C Kastanis, G Maounounen-Laasri, A De Jesus, AJ Curry, PE Stones, R K'Aluoch, O Liu, E Salter, M Hammack, TS Evans, PS Parish, M Allard, MW Datta, A Strain, EA Brown, EW AF Chen, Yi Burall, Laurel S. Luo, Yan Timme, Ruth Melka, David Muruvanda, Tim Payne, Justin Wang, Charles Kastanis, George Maounounen-Laasri, Anna De Jesus, Antonio J. Curry, Phillip E. Stones, Robert K'Aluoch, Okumu Liu, Eileen Salter, Monique Hammack, Thomas S. Evans, Peter S. Parish, Mickey Allard, Marc W. Datta, Atin Strain, Errol A. Brown, Eric W. TI Listeria monocytogenes in Stone Fruits Linked to a Multistate Outbreak: Enumeration of Cells and Whole-Genome Sequencing SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID SINGLE-NUCLEOTIDE POLYMORPHISMS; UNITED-STATES; PROCESSING PLANTS; EPIDEMIC CLONES; DATABASE; TIME; SALMONELLA; ALIGNMENT; STRAINS; SCHEME AB In 2014, the identification of stone fruits contaminated with Listeria monocytogenes led to the subsequent identification of a multistate outbreak. Simultaneous detection and enumeration of L. monocytogenes were performed on 105 fruits, each weighing 127 to 145 g, collected from 7 contaminated lots. The results showed that 53.3% of the fruits yielded L. monocytogenes (lower limit of detection, 5 CFU/fruit), and the levels ranged from 5 to 2,850 CFU/fruit, with a geometric mean of 11.3 CFU/fruit (0.1 CFU/g of fruit). Two serotypes, IVb-v1 and 1/2b, were identified by a combination of PCR- and antiserum-based serotyping among isolates from fruits and their packing environment; certain fruits contained a mixture of both serotypes. Single nucleotide polymorphism (SNP)-based whole-genome sequencing (WGS) analysis clustered isolates from two case-patients with the serotype IVb-v1 isolates and distinguished outbreak-associated isolates from pulsed-field gel electrophoresis (PFGE)-matched, but epidemiologically unrelated, clinical isolates. The outbreak-associated isolates differed by up to 42 SNPs. All but one serotype 1/2b isolate formed another WGS cluster and differed by up to 17 SNPs. Fully closed genomes of isolates from the stone fruits were used as references to maximize the resolution and to increase our confidence in prophage analysis. Putative prophages were conserved among isolates of each WGS cluster. All serotype IVb-v1 isolates belonged to singleton sequence type 382 (ST382); all but one serotype 1/2b isolate belonged to clonal complex 5. IMPORTANCE WGS proved to be an excellent tool to assist in the epidemiologic investigation of listeriosis outbreaks. The comparison at the genome level contributed to our understanding of the genetic diversity and variations among isolates involved in an outbreak or isolates associated with food and environmental samples from one facility. Fully closed genomes increased our confidence in the identification and comparison of accessory genomes. The diversity among the outbreak-associated isolates and the inclusion of PFGE-matched, but epidemiologically unrelated, isolates demonstrate the high resolution of WGS. The prevalence and enumeration data could contribute to our further understanding of the risk associated with Listeria monocytogenes contamination, especially among high-risk populations. C1 [Chen, Yi; Luo, Yan; Timme, Ruth; Melka, David; Muruvanda, Tim; Payne, Justin; Wang, Charles; Kastanis, George; Maounounen-Laasri, Anna; De Jesus, Antonio J.; Curry, Phillip E.; Salter, Monique; Hammack, Thomas S.; Evans, Peter S.; Parish, Mickey; Allard, Marc W.; Strain, Errol A.; Brown, Eric W.] US FDA, Ctr Food Safety & Appl Nutr, College Pk, MD 20740 USA. [Burall, Laurel S.; Datta, Atin] US FDA, Ctr Food Safety & Appl Nutr, Laurel, MD USA. [Stones, Robert] Newcastle Univ, Newcastle Upon Tyne, Tyne & Wear, England. [K'Aluoch, Okumu; Liu, Eileen] US FDA, Off Regulatory Affairs, Alameda, CA USA. [Wang, Charles; Evans, Peter S.] USDA, Washington, DC 20250 USA. RP Chen, Y (reprint author), US FDA, Ctr Food Safety & Appl Nutr, College Pk, MD 20740 USA. EM yi.chen@fda.hhs.gov FU DOE\ LDRD \ Oak Ridge Institute for Science and Education (ORISE) FX This work, including the efforts of Anna Maounounen-Laasri, was funded in part by DOE vertical bar LDRD vertical bar Oak Ridge Institute for Science and Education (ORISE). NR 55 TC 0 Z9 0 U1 11 U2 11 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD DEC PY 2016 VL 82 IS 24 BP 7030 EP 7040 DI 10.1128/AEM.01486-16 PG 11 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA EC4IH UT WOS:000388090500003 PM 27694232 ER PT J AU Agga, GE Schmidt, JW Arthur, TM AF Agga, Getahun E. Schmidt, John W. Arthur, Terrance M. TI Effects of In-Feed Chlortetracycline Prophylaxis in Beef Cattle on Animal Health and Antimicrobial-Resistant Escherichia coli SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID BOVINE RESPIRATORY-DISEASE; BLA(CMY-2)-POSITIVE INCA/C PLASMIDS; FOOD ANIMALS; SALMONELLA-ENTERICA; OUTER-MEMBRANE; BACTERIA; RISK; ANTIBIOTICS; IMPACTS; CONTAMINATION AB Concerns have been raised that in-feed chlortetracycline (CTC) may increase antimicrobial resistance (AMR), specifically tetracycline-resistant (TETr) Escherichia coli and third-generation cephalosporin-resistant (3GC(r)) E. coli. We evaluated the impact of a 5-day in-feed CTC prophylaxis on animal health, TETr E. coli, and 3GC(r) E. coli. A control group of cattle (n = 150) received no CTC, while a CTC group (n = 150) received in-feed CTC (10 mg/1b of body weight/day) from the 5th to the 9th day after feedlot arrival. Over 25% (38/150) of the animals in the control group developed illnesses requiring therapeutic treatment with antimicrobials critically important to human medicine. Only two animals (1.3%) in the CTC group required such treatments. Fecal swab and pen surface occurrences of generic E. coli (isolated on media that did not contain antimicrobials of interest and were not isolated based on any specific resistance), TETr E. coli, and 3GC(r) E. coli were determined on five sampling occasions: arrival at the feedlot, 5 days posttreatment (5 dpt), 27 dpt, 75 dpt, and 117 dpt. On 5 dpt, TETr E. coli concentrations were higher for the CTC group than the control group (P < 0.01). On 27 dpt, 75 dpt, and 117 dpt, TETr E. coli concentrations did not differ between groups. 3GC(r) E. coli occurrences did not differ between control and CTC groups on any sampling occasion. For both groups, generic, TETr, and 3GC(r) E. coli occurrences were highest on 75 dpt and 117 dpt, suggesting that factors other than in-feed CTC contributed more significantly to antimicrobial-resistant E. coli occurrence. IMPORTANCE The occurrence of human bacterial infections resistant to antimicrobial therapy has been increasing. It has been postulated that antimicrobial resistance was inevitable, but the life span of the antimicrobial era has been prematurely compromised due to the misuse of antimicrobials in clinical and agricultural practices. Direct evidence relating the use of antimicrobials in livestock production to diminished human health outcomes due to antimicrobial resistance is lacking, and the U.S. Food and Drug Administration has taken an approach to maximize therapeutic efficacy and minimize the selection of resistant microorganisms through judicious use of antimicrobials. This study demonstrated that prophylactic in-feed treatment of chlortetracycline administered for 5 days to calves entering feedlots is judicious, as this therapy reduced animal morbidity, reduced the use of antimicrobials more critical to human health, and had no long-term impact on the occurrence of antimicrobial-resistant E. coli. C1 [Agga, Getahun E.; Schmidt, John W.; Arthur, Terrance M.] ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Arthur, TM (reprint author), ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM Terrance.Arthur@ars.usda.gov NR 45 TC 0 Z9 0 U1 12 U2 12 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD DEC PY 2016 VL 82 IS 24 BP 7197 EP 7204 DI 10.1128/AEM.01928-16 PG 8 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA EC4IH UT WOS:000388090500019 PM 27736789 ER PT J AU Evans, JJ Pasnik, DJ Klesius, PH AF Evans, Joyce J. Pasnik, David J. Klesius, Phillip H. TI Colorimetric detection of Streptococcus agalactiae from fish isolates and clinical specimens SO AQUACULTURE RESEARCH LA English DT Article DE Streptococcus agalactiae; GBS; fish; bacterial identification; water ID NONHEMOLYTIC GROUP-B; CARROT BROTH; PCR; INFECTIONS; DIFFICILE; BOVINE; KUWAIT; RDNA; IB C1 [Evans, Joyce J.; Pasnik, David J.] USDA ARS, Aquat Anim Hlth Res Lab, Chestertown, MD USA. [Klesius, Phillip H.] USDA ARS, Aquat Anim Hlth Res Lab, Auburn, AL USA. RP Evans, JJ (reprint author), ARS, Warm Water Aquaculture Res Unit, USDA, 141 Expt Stn Rd, Stoneville, MS 38776 USA. EM joyce.evans55@yahoo.com NR 17 TC 0 Z9 0 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1355-557X EI 1365-2109 J9 AQUAC RES JI Aquac. Res. PD DEC PY 2016 VL 47 IS 12 BP 4043 EP 4048 DI 10.1111/are.12830 PG 6 WC Fisheries SC Fisheries GA EB8TD UT WOS:000387663000035 ER PT J AU DeWalle, DR Boyer, EW Buda, AR AF DeWalle, David R. Boyer, Elizabeth W. Buda, Anthony R. TI Exploring lag times between monthly atmospheric deposition and stream chemistry in Appalachian forests using cross-correlation SO ATMOSPHERIC ENVIRONMENT LA English DT Article; Proceedings Paper CT 9th International Acid Rain Conference (Acid Rain) CY OCT, 2015 CL Rochester, NY DE Lag times; Atmospheric deposition; Pre-whitening; Autocorrelation; Systems cross-correlation; Stream chemistry; Environmental monitoring ID NORTHEASTERN UNITED-STATES; ACIDIC DEPOSITION; WATER-QUALITY; SULFUR DEPOSITION; ADIRONDACK REGION; MID-APPALACHIANS; NEW-YORK; NITROGEN; TERM; ACIDIFICATION AB Forecasts of ecosystem changes due to variations in atmospheric emissions policies require a fundamental understanding of lag times between changes in chemical inputs and watershed response. Impacts of changes in atmospheric deposition in the United States have been documented using national and regional long-term environmental monitoring programs beginning several decades ago. Consequently, time series of weekly NADP atmospheric wet deposition and monthly EPA-Long Term Monitoring stream chemistry now exist for much of the Northeast which may provide insights into lag times. In this study of Appalachian forest basins, we estimated lag times for S, N and Cl by cross-correlating monthly data from four pairs of stream and deposition monitoring sites during the period from 1978 to 2012. A systems or impulse response function approach to cross-correlation was used to estimate lag times where the input deposition time series was pre-whitened using regression modeling and the stream response time series was filtered using the deposition regression model prior to cross-correlation. Cross-correlations for S were greatest at annual intervals over a relatively well-defined range of lags with the maximum correlations occurring at mean lags of 48 months. Chloride results were similar but more erratic with a mean lag of 57 months. Few high-correlation lags for N were indicated. Given the growing availability of atmospheric deposition and surface water chemistry monitoring data and our results for four Appalachian basins, further testing of cross-correlation as a method of estimating lag times on other basins appears justified. (C) 2016 Elsevier Ltd. All rights reserved. C1 [DeWalle, David R.; Boyer, Elizabeth W.] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [Buda, Anthony R.] ARS, Pasture Syst & Watershed Management Res Unit, USDA, University Pk, PA 16802 USA. RP DeWalle, DR (reprint author), Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. EM d9d@psu.edu NR 47 TC 0 Z9 0 U1 4 U2 4 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD DEC PY 2016 VL 146 SI SI BP 206 EP 214 DI 10.1016/j.atmosenv.2016.09.015 PG 9 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA EC3UG UT WOS:000388051700019 ER PT J AU Sun, X Zhao, ZF Zeng, FF Zhang, A Lu, ZX Wang, MQ AF Sun, X. Zhao, Z. -F. Zeng, F. -F. Zhang, A. Lu, Z. -X. Wang, M. -Q. TI Functional characterization of a pheromone-binding protein from rice leaffolder Cnaphalocrocis medinalis in detecting pheromones and host plant volatiles SO BULLETIN OF ENTOMOLOGICAL RESEARCH LA English DT Article DE Cnaphalocrocis medinalis; pheromone-binding proteins; immunocytochemistry; fluorescence competitive binding assays; trichoid sensilla ID BLACK CUTWORM MOTH; ODORANT-BINDING; SEX-PHEROMONE; ANTHERAEA-POLYPHEMUS; BOMBYX-MORI; AGROTIS-IPSILON; LEPIDOPTERA; EXPRESSION; ANTENNAE; IMMUNOLOCALIZATION AB Pheromone-binding proteins (PBPs) are believed to be involved in the recognition of semiochemicals. In the present study, western blot analysis, fluorescence-binding characteristics and immunolocalization of CmedPBP4 from the rice leaffolder, Cnaphalocrocis medinalis, were investigated. Western blot analysis revealed that CmedPBP4 showed obvious antenna-specific expression patterns in female and male antenna, and made a clearly different sex-biased expression. Immunocytochemical labeling revealed that CmedPBP4 showed specific expression in the trichoid sensilla. Competitive fluorescence binding assays indicated that CmedPBP4 could selectively recognize three sex pheromone components (Z13-18: Ac, Z11-16: Al and Z13-18: OH) and eleven rice plant volatiles, including cyclohexanol, nerolidol, cedrol, dodecanal, ionone, (-)-alpha-cedrene, (Z)-farnesene, beta-myrcene, R-(+)-limonene, (-)-limonene, and (+)-3-carene. Meanwhile the CmedPBP4 detection of sex pheromones and host odorants was pH-dependent. Our results, for the first time, provide further evidence that trichoid sensilla might be play an important role in detecting sex pheromones and host plant volatiles in the C. medinalis moth. Our systematic studies provided further detailed evidence for the function of trichoid sensilla in insect semiochemical perception. C1 [Sun, X.; Zhao, Z. -F.; Zeng, F. -F.; Wang, M. -Q.] Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan, Peoples R China. [Sun, X.] Henan Univ, Coll Life Sci, Key Lab Invas Ecol, Kaifeng, Henan, Peoples R China. [Zhang, A.] USDA ARS, BARC West, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. [Lu, Z. -X.] Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, Hangzhou, Zhejiang, Peoples R China. RP Wang, MQ (reprint author), Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan, Peoples R China. EM mqwang@mail.hzau.edu.cn FU National High Technology Research and Development Program of China (863 Program) [2014AA10A605]; China Agriculture Research System [CARS-01-17]; Fundamental Research Funds for the Central Universities [2013PY046] FX This study was supported and funded by the National High Technology Research and Development Program of China (863 Program) (Grant no. 2014AA10A605), China Agriculture Research System (Grant no. CARS-01-17) and Fundamental Research Funds for the Central Universities (Grant no. 2013PY046). NR 58 TC 0 Z9 0 U1 24 U2 24 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-4853 EI 1475-2670 J9 B ENTOMOL RES JI Bull. Entomol. Res. PD DEC PY 2016 VL 106 IS 6 BP 781 EP 789 DI 10.1017/S0007485316000560 PG 9 WC Entomology SC Entomology GA EC2VB UT WOS:000387981200009 PM 27385278 ER PT J AU Stokland, JN Woodall, CW Fridman, J Stahl, G AF Stokland, Jogeir N. Woodall, Christopher W. Fridman, Jonas Stahl, Goran TI Burial of downed deadwood is strongly affected by log attributes, forest ground vegetation, edaphic conditions, and climate zones SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article; Proceedings Paper CT 17th Conference of the International-Boreal-Forest-Research- Association (IBFRA) CY MAY 24-29, 2015 CL Rovaniemi, FINLAND SP Int Boreal Forest Res Assoc, Nat Resources Inst Finland DE buried wood; coarse woody debris; greenhouse gas accounting; wood decomposition; soil carbon ID COARSE WOODY DEBRIS; UNITED-STATES; CARBON; DECOMPOSITION; BIOMASS; GROWTH; SWEDEN; DECAY; CONVERSION; DYNAMICS AB Deadwood can represent a substantial portion of forest ecosystem carbon stocks and is often reported following good practice guidance associated with national greenhouse gas inventories. In high-latitude forest ecosystems, a substantial proportion of downed deadwood is overgrown by ground vegetation and buried in the humus layer. Such burial obfuscates the important process of deadwood carbon transfer to other pools (e.g., litter and soil) and emission to the atmosphere (i.e., rates of decay). Using data from the Swedish National Forest Inventory, we found that the proportion of downed logs that is buried increased from temperate to boreal forests. Several factors affect the probability of burial, including log attributes (e.g., decay class), ground vegetation (e.g., moss dominance, type of moss cover), and edaphic conditions (e.g., soil type, depth of organic layer). Combined assessments suggest that about 24% of the carbon in the aboveground downed deadwood pool was found to be buried in boreal forests. Deadwood burial has important implications for forest carbon dynamics and associated monitoring (e.g., United Nations Framework Convention on Climate Change reporting) as such a pool typically decomposes much slower compared with aboveground deadwood. C1 [Stokland, Jogeir N.] Norwegian Inst Bioecon Res, POB 115, NO-1431 As, Norway. [Woodall, Christopher W.] US Forest Serv, USDA, Res & Dev, Northern Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. [Fridman, Jonas; Stahl, Goran] Swedish Univ Agr Sci, Dept Forest Resource Management, S-90183 Umea, Sweden. RP Stokland, JN (reprint author), Norwegian Inst Bioecon Res, POB 115, NO-1431 As, Norway. EM Jogeir.Stokland@nibio.no NR 24 TC 0 Z9 0 U1 9 U2 9 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD DEC PY 2016 VL 46 IS 12 BP 1451 EP 1457 DI 10.1139/cjfr-2015-0461 PG 7 WC Forestry SC Forestry GA EC4JI UT WOS:000388094000005 ER PT J AU Calvo, P Watts, DB Kloepper, JW Torbert, HA AF Calvo, Pamela Watts, Dexter B. Kloepper, Joseph W. Torbert, H. Allen TI The influence of microbial-based inoculants on N2O emissions from soil planted with corn (Zea mays L.) under greenhouse conditions with different nitrogen fertilizer regimens SO CANADIAN JOURNAL OF MICROBIOLOGY LA English DT Article DE microbial inoculants; PGPR; SoilBuilder (TM); greenhouse gas emissions; N2O production ID GROWTH-PROMOTING RHIZOBACTERIA; OXIDE EMISSIONS; BACTERIA; MICROORGANISMS; AGRICULTURE; COMMUNITIES; ECOSYSTEM; SYSTEMS; IMPACT; PGPR AB Nitrous oxide (N2O) emissions are increasing at an unprecedented rate owing to the increased use of nitrogen (N) fertilizers. Thus, new innovative management tools are needed to reduce emissions. One potential approach is the use of microbial inoculants in agricultural production. In a previous incubation study, we observed reductions in N2O emissions when microbial-based inoculants were added to soil (no plants present) with N fertilizers under laboratory incubations. This present study evaluated the effects of microbial-based inoculants on N2O and carbon dioxide (CO2) emissions when applied to soil planted with corn (Zea mays L.) under controlled greenhouse conditions. Inoculant treatments consisted of (i) SoilBuilder (SB), (ii) a metabolite extract of SoilBuilder (SBF), and (iii) a mixture of 4 strains of plant-growth-promoting Bacillus spp. (BM). Experiments included an unfertilized control and 3 N fertilizers: urea, urea - ammonium nitrate with 32% N (UAN-32), and calcium - ammonium nitrate with 17% N(CAN-17). Cumulative N2O fluxes from pots 41 days after planting showed significant reductions in N2O of 15% (SB), 41% (BM), and 28% (SBF) with CAN-17 fertilizer. When UAN-32 was used, reductions of 34% (SB), 35% (SBF), and 49% (BM) were obtained. However, no reductions in N2O emissions occurred with urea. Microbial-based inoculants did not affect total CO2 emissions from any of the fertilized treatments or the unfertilized control. N uptake was increased by an average of 56% with microbial inoculants compared with the control (nonmicrobial-based treatments). Significant increases in plant height, SPAD chlorophyll readings, and fresh and dry shoot mass were also observed when the microbial-based treatments were applied (with and without N). Overall, results demonstrate that microbial inoculants can reduce N2O emissions following fertilizer application depending on the N fertilizer type used and can enhance N uptake and plant growth. Future studies are planned to evaluate the effectiveness of these microbial inoculants in field-based trials and determine the mechanisms involved in N2O reduction. C1 [Calvo, Pamela; Kloepper, Joseph W.] Auburn Univ, Entomol & Plant Pathol Dept, 209 Life Sci Bldg, Auburn, AL 36849 USA. [Watts, Dexter B.; Torbert, H. Allen] ARS, USDA, Natl Soil Dynam Lab, 411 S Donahue Dr, Auburn, AL 36832 USA. RP Calvo, P (reprint author), Auburn Univ, Entomol & Plant Pathol Dept, 209 Life Sci Bldg, Auburn, AL 36849 USA. EM pzc0006@tigermail.auburn.edu FU U.S. Department of Agriculture; Alabama Agricultural Experiment Station; Auburn University Department of Entomology and Plant Pathology; Agricen Sciences FX Financial support for this project was provided by the U.S. Department of Agriculture, Alabama Agricultural Experiment Station, Auburn University Department of Entomology and Plant Pathology, and Agricen Sciences. We acknowledge Robert Ames for suggestions during the planning and experimental stages of the study. We thank James Ashley Robinson for technical assistance and William Fowler for help with editing the manuscript. Mention of trade names or commercial products in this article are solely for the purpose of providing specific information and do not imply recommendation or endorsement by Auburn University and the U.S. Department of Agriculture. NR 50 TC 0 Z9 0 U1 21 U2 21 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0008-4166 EI 1480-3275 J9 CAN J MICROBIOL JI Can. J. Microbiol. PD DEC PY 2016 VL 62 IS 12 BP 1041 EP 1056 DI 10.1139/cjm-2016-0122 PG 16 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Immunology; Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Immunology; Microbiology GA EC4HM UT WOS:000388088000007 PM 27829287 ER PT J AU Qin, J Shi, AN Xiong, HZ Mou, BQ Motes, D Lu, WG Miller, JC Scheuring, DC Nzaramba, MN Weng, YJ Yang, W AF Qin, Jun Shi, Ainong Xiong, Haizheng Mou, Beiquan Motes, Dennis Lu, Weiguo Miller, J. Creighton, Jr. Scheuring, Douglas C. Nzaramba, M. Ndambe Weng, Yuejin Yang, Wei TI Population structure analysis and association mapping of seed antioxidant content in USDA cowpea (Vigna unguiculata L. Walp.) core collection using SNPs SO CANADIAN JOURNAL OF PLANT SCIENCE LA English DT Article DE cowpea; Vigna unguiculata; seed antioxidant content; seed colour; single nucleotide polymorphism (SNP); association analysis ID GENOME-WIDE ASSOCIATION; COMPLEX TRAITS; HEART-DISEASE; FREE-RADICALS; RESISTANCE; DIVERSITY; MAIZE; LINKAGE; CONSUMPTION; GERMPLASM AB Cowpea (Vigna unguiculata L. Walp.) is an important legume, and the antioxidant content in cowpea seeds has been recognized as a health-promoting compound for humans. The objectives of this study were to analyze the population structure of cowpea collections and to identify single nucleotide polymorphism (SNP) markers associated with the seed antioxidant content and seed coat colour. A set of 1047 SNPs were used to analyze a 369 cowpea core collection from 47 countries. Results indicated that: (1) there were three clusters in the 369 entries; and the germplasm collected from India, South Africa, and the US showed broader genetic diversity; (2) Scaffold7139_14363 and Scaffold29110_4657 were strongly associated with antioxidant content, and C35063613_1497, Scaffold81493_886, and Scaffold84620_6785 were strongly associated with seed coat colour across three models; (3) significant correlations were detected between the seed antioxidant content and black seed colour (r = 0.45) and between seed antioxidant content and red seed coat colour (r = 0.50); and (4) Scaffold42008_191 and C35082838_2258 were associated with both seed antioxidant content and seed coat colour. The SNP markers identified could potentially be used in marker-assisted breeding to accelerate genetic improvement of cowpea for higher seed antioxidant content. C1 [Qin, Jun; Shi, Ainong; Xiong, Haizheng; Lu, Weiguo; Weng, Yuejin; Yang, Wei] Univ Arkansas, Dept Hort, Fayetteville, AR 72701 USA. [Qin, Jun] Hebei Acad Agr & Forestry Sci, North China Key Lab Biol & Genet Improvement Soyb, Natl Soybean Improvement Ctr,Minist Agr, Shijiazhuang Subctr,Cereal & Oil Crop Inst, Shijiazhuang 050031, Peoples R China. [Mou, Beiquan] ARS, Crop Improvement & Protect Res Unit, USDA, 1636 E Alisal St, Salinas, CA 93905 USA. [Motes, Dennis] Univ Arkansas, Vegetable Res Ctr, Alma, AR 72921 USA. [Miller, J. Creighton, Jr.; Scheuring, Douglas C.] Texas A&M Univ, Dept Hort Sci, College Stn, TX 77843 USA. [Nzaramba, M. Ndambe] Minist Agr & Anim Resources, BP 621, Kigali, Rwanda. RP Shi, AN (reprint author), Univ Arkansas, Dept Hort, Fayetteville, AR 72701 USA. EM ashi@uark.edu FU USDA-ARS GRIN GERMPLASM EVALUATION PROPOSAL for National Plant Germplasm System (NPGS) by Crop Germplasm Committee (CGC) [6046-21000-011-15] FX This work was supported by USDA-ARS GRIN GERMPLASM EVALUATION PROPOSAL for National Plant Germplasm System (NPGS) by Crop Germplasm Committee (CGC) with Project Number: 6046-21000-011-15. Cowpea germplasm accessions were provided by USDA-ARS at GRIFFIN, GA Station. The cowpea_Genome_0.03.fa (6750 scaffolds or contigs) (http://harvest-blast.org/) was kindly provided by Dr. Timothy J. Close at the University of California Riverside, CA, USA. NR 53 TC 1 Z9 1 U1 9 U2 9 PU AGRICULTURAL INST CANADA PI OTTAWA PA 280 ALBERT ST, SUITE 900, OTTAWA, ONTARIO K1P 5G8, CANADA SN 0008-4220 EI 1918-1833 J9 CAN J PLANT SCI JI Can. J. Plant Sci. PD DEC PY 2016 VL 96 IS 6 BP 1026 EP 1036 DI 10.1139/cjps-2016-0090 PG 11 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA EC4HY UT WOS:000388089400010 ER PT J AU Campbell, RM Venn, TJ Anderson, NM AF Campbell, Robert M. Venn, Tyron J. Anderson, Nathaniel M. TI Social preferences toward energy generation with woody biomass from public forests in Montana, USA SO FOREST POLICY AND ECONOMICS LA English DT Article DE Renewable energy; Woody biomass; Choice modeling; Willingness to pay ID WILLINGNESS-TO-PAY; CHOICE EXPERIMENTS; FOSSIL-FUELS; ENVIRONMENTAL IMPACTS; MANAGEMENT-PRACTICES; VEGETATION STRUCTURE; RENEWABLE ENERGY; PRESCRIBED FIRE; UNITED-STATES; COLORADO USA AB In Montana, USA, there are substantial opportunities for mechanized thinning treatments on public forests to reduce the likelihood of severe and damaging wildfires and improve forest health. These treatments produce residues that can be used to generate renewable energy and displace fossil fuels. The choice modeling method is employed to examine the marginal willingness of Montanans' to pay (MWTP) for woody biomass energy produced from treatments in their public forests. The survey instrument elicited social preferences for important co-benefits and costs of woody biomass energy generation in Montana, namely the extent of healthy forests, the number of large wildfires, and local air quality. Positive and statistically significant MWTP is found for woody biomass energy generation, forest health and air quality. MWTP to avoid large wildfires is statistically insignificant. However, MW FP for woody biomass energy diminishes quickly, revealing that Montanans do not support public forestland management that produces more than double the current level of woody biomass harvested for energy generation. These findings can be used by policy makers and public land managers to estimate the social benefits of utilizing residues from public forest restoration or fuel treatment programs to generate energy. (C) 2016 Elsevier B.V. All rights reserved. C1 [Campbell, Robert M.; Venn, Tyron J.] Univ Montana, Coll Forestry & Conservat, 32 Campus Dr, Missoula, MT 59812 USA. [Venn, Tyron J.] Univ Sunshine Coast, Sch Business, Sippy Downs, Qld 4556, Australia. [Anderson, Nathaniel M.] US Forest Serv, Rocky Mt Res Stn, 800 East Beckwith, Missoula, MT 59801 USA. RP Campbell, RM (reprint author), Univ Montana, Coll Forestry & Conservat, 32 Campus Dr, Missoula, MT 59812 USA. EM robert3.campbell@umontana.edu; tvenn@usc.edu.au; nathanielmanderson@fs.fed.us NR 70 TC 0 Z9 0 U1 7 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1389-9341 EI 1872-7050 J9 FOREST POLICY ECON JI Forest Policy Econ. PD DEC PY 2016 VL 73 BP 58 EP 67 DI 10.1016/j.forpol.2016.08.008 PG 10 WC Forestry SC Forestry GA EC3XH UT WOS:000388059900007 ER PT J AU Brand, D Wijewardana, C Gao, W Reddy, KR AF Brand, David Wijewardana, Chathurika Gao, Wei Reddy, K. Raja TI Interactive effects of carbon dioxide, low temperature, and ultraviolet-B radiation on cotton seedling root and shoot morphology and growth SO FRONTIERS OF EARTH SCIENCE LA English DT Article DE Cotton; Climate change; winRHIZO; Principal component analysis.; Stress tolerance ID GOSSYPIUM-HIRSUTUM L.; AIR CO2 ENRICHMENT; HYPERSPECTRAL REFLECTANCE; HEAT TOLERANCE; RESPONSES; COLD; PHOTOSYNTHESIS; LEAVES AB Interactive effects of multiple environmental stresses are predicted to have a negative effect on cotton growth and development and these effects will be exacerbated in the future climate. The objectives of this study were to test the hypothesis that cotton cultivars differ in their responses to multiple environmental factors of (CO2) [400 and 750 A mu mol center dot mol(-1) (+(CO2)], temperature [28/20 and 20/12A degrees C (-T)], and UV-B radiation [0 and 10 kJ center dot m(-2)center dot d(-1) (+ UV-B)]. A genetic and molecular standard (TM-1) and three modern cotton cultivars (DP1522B2XF, PHY496W3R, and ST4747GLB2) were grown in eight sunlit, controlled environment chambers with control treatment 400 A mu mol center dot mol(-1) [CO2], 28/21A degrees C temperature, and 0 kJ UV-B. The results showed significant differences among the cultivars for most of the shoot and root parameters. Plants grown under low temperature alone or as a combination with + UV-B treatment caused more detrimental effects on root and shoot vigor. Although the elevated CO2 treatments weakened the damaging effects of higher UV-B levels on cotton growth on all cultivars, increased CO2 could not mask the negative effects of low temperature. When comparing all cultivars, genetic standard TM-1 produced the smallest values for the majority of traits under CO2, UV-B, and low temperature either alone or in combination with other treatments. Based on principal component analysis, the four cultivars were classified as tolerant (DP1522B2XF), intermediate (PHY496W3R and ST4747GLB2) and sensitive (TM-1) to multiple environmental stresses.Low temperature was identified as the most damaging treatment to cotton early seedling vigor while elevated CO2 caused the least. Existing variability of cotton cultivars in response to multiple environmental stresses could allow for selection of cultivars with the best coping ability and higher lint yield for future climate change environments. C1 [Brand, David; Wijewardana, Chathurika; Reddy, K. Raja] Mississippi State Univ, Dept Plant & Soil Sci, Starkwille, MS 39762 USA. [Gao, Wei] Colorado State Univ, Nat Resource Ecol Lab, USDA UVB Monitoring & Res Program, Ft Collins, CO 80523 USA. [Gao, Wei] Colorado State Univ, Dept Ecosyst Sci & Sustainabil, Ft Collins, CO 80523 USA. RP Reddy, KR (reprint author), Mississippi State Univ, Dept Plant & Soil Sci, Starkwille, MS 39762 USA. EM krreddy@pss.msstate.edu FU USDA NIFA [2014-34263-22038, G-89701-1, 2015-34263-24070, G-14901-1]; Cotton Incorporated, Inc., USA FX This work is partially supported by the USDA NIFA projects (2014-34263-22038, G-89701-1 and 2015-34263-24070, G-14901-1) and the Cotton Incorporated, Inc., USA. We would like to thank fellow graduate students for their help in taking measurements and processing samples in the lab. This study is a contribution of the Department of Plant and Soil Sciences, Mississippi State University, and the Mississippi Agricultural and Forestry Experiment Station. NR 46 TC 0 Z9 0 U1 18 U2 18 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 2095-0195 EI 2095-0209 J9 FRONT EARTH SCI-PRC JI Front. Earth Sci. PD DEC PY 2016 VL 10 IS 4 BP 607 EP 620 DI 10.1007/s11707-016-0605-0 PG 14 WC Geosciences, Multidisciplinary SC Geology GA EC1LJ UT WOS:000387866300001 ER PT J AU Shi, M Zhao, S Wang, ZH Stanley, D Chen, XX AF Shi, M. Zhao, S. Wang, Z. -H. Stanley, D. Chen, X. -X. TI Cotesia vestalis parasitization suppresses expression of a Plutella xylostella thioredoxin SO INSECT MOLECULAR BIOLOGY LA English DT Article DE thioredoxin; Plutella xylostella; expression profile; antioxidant capacity; parasitization ID DROSOPHILA-MELANOGASTER; MOLECULAR-CLONING; RUDITAPES-PHILIPPINARUM; RECOMBINANT PROTEIN; REDOX REGULATION; ANTIOXIDANT; REDUCTASE; SYSTEM; INFECTION; SILKWORM AB Thioredoxins (Trxs) are a family of small, highly conserved and ubiquitous proteins involved in protecting organisms against toxic reactive oxygen species. In this study, a typical thioredoxin gene, PxTrx, was isolated from Plutella xylostella. The full-length cDNA sequence is composed of 959 bp containing a 321 bp open reading frame that encodes a predicted protein of 106 amino acids, a predicted molecular weight of 11.7 kDa and an isoelectric point of 5.03. PxTrx was mainly expressed in larval Malpighian tubules and the fat body. An enriched recombinant PxTrx had insulin disulphide reductase activity and stimulated Human Embryonic Kidney 293 (HEK293) cell proliferation. It also protected supercoiled DNA and living HEK293 cells from H2O2-induced damage. Parasitization by Cotesia vestalis and injections of 0.05 and 0.01 equivalents of C. vestalis Bracovirus (CvBv), the symbiotic virus carried by the parasitoid, led to down-regulation of PxTrx expression in host fat body. Taken together, our results indicate that PxTrx contributes to the maintenance of P. xylostella cellular haemostasis. Host fat body expression of PxTrx is strongly attenuated by parasitization and by injections of CvBv. C1 [Shi, M.; Zhao, S.; Wang, Z. -H.; Chen, X. -X.] Zhejiang Univ, Inst Insect Sci, Minist Agr, Key Lab Agr Entomol, 866 Yuhangtang Rd, Hangzhou 310058, Zhejiang, Peoples R China. [Stanley, D.] ARS, Biol Control Insects Res Lab, USDA, Columbia, MO USA. RP Chen, XX (reprint author), Zhejiang Univ, Inst Insect Sci, Minist Agr, Key Lab Agr Entomol, 866 Yuhangtang Rd, Hangzhou 310058, Zhejiang, Peoples R China. EM xxchen@zju.edu.cn RI Chen, Xue-xin/D-1592-2010 OI Chen, Xue-xin/0000-0002-9109-8853 FU 973 Program [2013CB127600]; National Science Fund for Innovative Research Groups of China [31321063]; Zhejiang Science Fund for Distinguished Young Scholars [R3110049] FX Funding for this study was provided jointly by the 973 Program (2013CB127600) and the National Science Fund for Innovative Research Groups of China (31321063) to X-X.C., and the Zhejiang Science Fund for Distinguished Young Scholars (R3110049) to M.S. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. All programs and services of the U.S. Department of Agriculture are offered on a nondiscriminatory basis without regard to race, colour, national origin, religion, sex, age, marital status, or handicap. NR 41 TC 0 Z9 0 U1 12 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1075 EI 1365-2583 J9 INSECT MOL BIOL JI Insect Mol. Biol. PD DEC PY 2016 VL 25 IS 6 BP 679 EP 688 DI 10.1111/imb.12252 PG 10 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA EB8RZ UT WOS:000387659900001 PM 27376399 ER PT J AU Sun, X Zeng, FF Yan, MJ Zhang, A Lu, ZX Wang, MQ AF Sun, X. Zeng, F. -F. Yan, M. -J. Zhang, A. Lu, Z. -X. Wang, M. -Q. TI Interactions of two odorant-binding proteins influence insect chemoreception SO INSECT MOLECULAR BIOLOGY LA English DT Article DE odorant-binding protein; Cnaphalocrocis medinalis; molecular docking; fluorescence competitive binding assays; RNA interference; interactions; electroantennogram responses ID INDUCE VOLATILE EMISSIONS; DROSOPHILA-MELANOGASTER; ANOPHELES-GAMBIAE; LYGUS-HESPERUS; SWISS-MODEL; ELECTROPHYSIOLOGICAL RESPONSES; CNAPHALOCROCIS-MEDINALIS; EXPRESSION PATTERN; PHEROMONE-BINDING; LIGAND-BINDING AB It is well known that the odorant-binding proteins (OBPs) play crucial roles in insect olfactory detection. To explore if interactions of OBPs affect olfactory coding in the rice leaffolder Cnaphalocrocis medinalis ligand-binding experiments, molecular docking, RNA interference and electrophysiological recording were performed. The binding activity of two C. medinalis OBPs (CmedOBPs) to rice plant volatiles showed wide flexibility depending on the structure of ligands and interactions of CmedOBPs involved. The binding sites of CmedOBP2 and CmedOBP3 to rice plant volatiles were well predicted by three-dimensional structure modelling and molecular docking experiments. In addition, the interactions of these two CmedOBPs in the perception of rice volatiles were demonstrated by RNA interference experiments. When a single double-stranded RNA (dsRNA)-Cme-dOBP2 was injected, the expression of CmedOBP2 was significantly reduced and the expression of CmedOBP3 was significantly increased, and vice versa. When both dsRNA-CmedOBP2 and 3 were injected together, greater reduction of electroantennogram responses to rice plant volatiles was induced than that seen with individual injection of either dsRNA-CmedOBP2 or dsRNA-CmedOBP3. These results clearly indicate that the interactions of CmedOBP2 and CmedOBP3 have significant effects on C. medinalis during the detection of host plant volatiles. C1 [Sun, X.; Zeng, F. -F.; Yan, M. -J.; Wang, M. -Q.] Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Peoples R China. [Sun, X.] Henan Univ, Coll Life Sci, Key Lab Invas Ecol, Kaifeng, Henan, Peoples R China. [Zhang, A.] USDA ARS, BARC West, Invas Insect Biocontrol & Behav Lab, Beltsville, MD USA. [Lu, Z. -X.] Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, Hangzhou, Zhejiang, Peoples R China. RP Wang, MQ (reprint author), Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Peoples R China. EM mqwang@mail.hzau.edu.cn FU Special Fund for Agro-scientific Research in the Public Interest of China [201403030]; China Agriculture Research System [CARS-01-17]; Fundamental Research Funds for the Central Universities [2013PY046]; China Scholarship Council of China FX We thank Dr Emmanuelle Jacquin-Joly (French National Institute for Agronomic Research, Versailles) for assistance with editorial comments. This study was supported and funded by the Special Fund for Agro-scientific Research in the Public Interest of China (201403030), China Agriculture Research System (grant no. CARS-01-17) and Fundamental Research Funds for the Central Universities (2013PY046). X.S.'s work was generously supported by the China Scholarship Council of China. The authors have declared that no competing interest exists. NR 60 TC 0 Z9 0 U1 13 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1075 EI 1365-2583 J9 INSECT MOL BIOL JI Insect Mol. Biol. PD DEC PY 2016 VL 25 IS 6 BP 712 EP 723 DI 10.1111/imb.12256 PG 12 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA EB8RZ UT WOS:000387659900004 PM 27503414 ER PT J AU Smith, DM Finch, DM AF Smith, D. Max Finch, Deborah M. TI Riparian trees and aridland streams of the southwestern United States: An assessment of the past, present, and future SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Aridlands; Climate change; Southwest; Stream; Regulation; Riparian; Woody vegetation ID COLORADO RIVER-BASIN; WESTERN NORTH-AMERICA; MIDDLE RIO-GRANDE; SAN-PEDRO RIVER; CENTRAL NEW-MEXICO; TAMARIX-RAMOSISSIMA; PLATANUS-WRIGHTII; CENTRAL ARIZONA; CLIMATE-CHANGE; RUSSIAN-OLIVE AB Riparian ecosystems are vital components of aridlands within the southwestern United States. Historically, surface flows influenced population dynamics of native riparian trees. Many southwestern streams has been altered by regulation, however, and will be further affected by greenhouse warming. Our analysis of stream gage data revealed that decreases in volume of annual discharge and mean peak discharge and a shift to earlier peak discharge will occur in the Southern Rockies region of Colorado, New Mexico, and Utah. These changes will likely decrease rates of reproduction and survival of cottonwood (Populus fremontii and Populus deltoides ssp. wislizenii), Goodding's willow (Salix gooddingii), and box- elder (Acer negundo), which rely on surface flows to stimulate germination and recharge groundwater aquifers. Streams in the Central Highlands of Arizona and New Mexico will likely see reductions in annual discharge volume, which could limit reproduction and survival of the above taxa and Arizona sycamore (Platanus wrightii). These effects may be exacerbated by demands of expanding urban areas and agricultural operations, but could also be ameliorated by increasing water use efficiency and environmental mitigation. These factors must be considered, along with climate projections, when planning for conservation of riparian trees and the animal communities they support. (C) 2016 Published by Elsevier Ltd. C1 [Smith, D. Max; Finch, Deborah M.] US Forest Serv, USDA, Rocky Mt Res Stn, 333 Broadway SE,Suite 115, Albuquerque, NM 87102 USA. RP Smith, DM (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, 333 Broadway SE,Suite 115, Albuquerque, NM 87102 USA. EM dms6@pdx.edu FU Desert and Southern Rockies Landscape Conservation Cooperatives [R12PG8047]; Rocky Mountain Research Station's Climate Change Research Program FX Funding for this work was provided by the Desert and Southern Rockies Landscape Conservation Cooperatives (R12PG8047) and the Rocky Mountain Research Station's Climate Change Research Program. We acknowledge the modeling groups (listed in the Appendix), the Program for Climate Model Diagnosis and Inter comparison (PCMDI) and the WCRP's Working Group on Coupled Modeling (WGCM) for their roles in making available the WCRP CMIP3 multi-model dataset. Support of this dataset is provided by the Office of Science, U.S. Department of Energy. NR 113 TC 0 Z9 0 U1 13 U2 13 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 EI 1095-922X J9 J ARID ENVIRON JI J. Arid. Environ. PD DEC PY 2016 VL 135 BP 120 EP 131 DI 10.1016/j.jaridenv.2016.08.016 PG 12 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA EC1AS UT WOS:000387836500016 ER PT J AU Nelson, CD Lippolis, JD Reinhardt, TA Sacco, RE Powell, JL Drewnoski, ME O'Neil, M Beitz, DC Weiss, WR AF Nelson, Corwin D. Lippolis, John D. Reinhardt, Timothy A. Sacco, Randy E. Powell, Jessi L. Drewnoski, Mary E. O'Neil, Matthew Beitz, Donald C. Weiss, William R. TI Vitamin D status of dairy cattle: Outcomes of current practices in the dairy industry SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE vitamin D; dairy cattle ID FAT-SOLUBLE VITAMINS; CALVES; COWS; HEALTH; GROWTH; SERUM; PARATUBERCULOSIS; REQUIREMENTS; METABOLISM; COLOSTRUM AB The need for vitamin D supplementation of dairy cattle has been known for the better part of the last century and is well appreciated by dairy producers and nutritionists. Whether current recommendations and practices for supplemental vitamin D are meeting the needs of dairy cattle, however, is not well known. The vitamin D status of animals is reliably indicated by the concentration of the 25-hydroxyvitamin D [25(011)D] metabolite in serum or plasma, with a concentration of 30 ng/mL proposed as a lower threshold for sufficiency. The objective of this study was to determine the typical serum 25(011)D concentrations of dairy cattle across various dairy operations. The serum 25(011) D concentration of 702 samples collected from cows across various stages of lactation, housing systems, and locations in the United States was 68 +/- 22 ng/ mL (mean +/- standard deviation), with the majority of samples between 40 and 100 ng/mL. Most of the 12 herds surveyed supplemented cows with 30,000 to 50,000 IU of vitamin D-3/d, and average serum 25(011) D of cows at 100 to 300 DIM in each of those herds was near or above 70 ng/mL regardless of season or housing. In contrast, average serum 25(011)D of a herd supplementing with 20,000 IU/d was 42 +/- 15 ng/mL, with 22% below 30 ng/mL. Cows in early lactation (0 to 30 d in milk) also had lower serum 25(OH)D than did mid- to late-lactation cows (57 +/- 17 vs. 71 +/- 20 ng/mL, respectively). Serum 25(OH)D of yearling heifers receiving 11,000 to 12,000 IU of vitamin D-3/d was near that of cows at 76 15 ng/mL. Serum 25(011)D concentrations of calves, on the other hand, was 15 +/- 11 ng/mL at birth and remained near or below 15 ng/ mL through 1 mo of age if they were fed pasteurized waste milk with little to no summer sun exposure. In contrast, serum 25(OH)D of calves fed milk replacer containing 6,600 and 11,000 IU of vitamin D-2/kg of dry matter were 59 +/- 8 and 98 +/- 33 ng/mL, respectively, at 1 mo of age. Experimental data from calves similarly indicated that serum 25(OH)D achieved at approximately 1 mo of age would increase 6 to 7 ng/mL for every 1,000 IU of vitamin D-3/kg of dry matter of milk replacer. In conclusion, vitamin D status of dairy cattle supplemented with vitamin D-3 according to typical practices, about 1.5 to 2.5 times the National Research Council recommendation, is sufficient as defined by serum 25(OH)D concentrations. Newborn calves and calves fed milk without supplemental vitamin D-3, however, are prone to deficiency. C1 [Nelson, Corwin D.; Powell, Jessi L.] Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA. [Lippolis, John D.; Reinhardt, Timothy A.; Sacco, Randy E.] USDA ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Drewnoski, Mary E.] Univ Nebraska, Dept Anim Sci, Lincoln, NE 68583 USA. [O'Neil, Matthew; Beitz, Donald C.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. [Weiss, William R.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Anim Sci, Wooster, OH 44691 USA. RP Nelson, CD (reprint author), Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA. EM cdnelson@ufl.edu NR 43 TC 0 Z9 0 U1 5 U2 5 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD DEC PY 2016 VL 99 IS 12 BP 10150 EP 10160 DI 10.3168/jds.2016-11727 PG 11 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA EC1BC UT WOS:000387837500070 PM 27743666 ER PT J AU Bautista, E AF Bautista, Eduardo TI Effect of Infiltration Modeling Approach on Operational Solutions for Furrow Irrigation SO JOURNAL OF IRRIGATION AND DRAINAGE ENGINEERING LA English DT Article ID SURFACE IRRIGATION; ADVANCE; SIMULATION; WINSRFR; SYSTEMS; WATER; FLOW AB Infiltration in irrigated furrows depends on the variation of depth of flow and, thus, wetted perimeter along the field and in time. However, the magnitude of the wetted perimeter effect has not been clearly established due to soil variability, erosion and deposition, macropore flow, and other processes that affect the infiltration process. Hence, furrow irrigation models currently offer various alternatives, mostly empirical but some physical, for modeling the infiltration process as a function of wetted perimeter. In principle, the selected modeling approach can have a significant impact on the predicted performance. This is of particular importance for operation and design analyses cases in which performance is examined, respectively, over a wide range of inflow rates and physical configurations. A simulation study was conducted to examine the operational solutions that can be developed with different furrow infiltration models. The analysis was based on the modeling options offered by a software package developed by U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS). The analysis was conducted under the assumption that the true infiltration characteristics of the example furrow were given by a semiphysically based infiltration model. Performance contours and operational solutions were developed for a free draining and blocked furrow. Results show that operational solutions can be very sensitive to the infiltration modeling approach with a free draining system, but less sensitive with blocked systems. (C) 2016 American Society of Civil Engineers. C1 [Bautista, Eduardo] USDA ARS, US Arid Land Agr Res Ctr, 21881 N Cardon Ln, Maricopa, AZ 85138 USA. RP Bautista, E (reprint author), USDA ARS, US Arid Land Agr Res Ctr, 21881 N Cardon Ln, Maricopa, AZ 85138 USA. EM Eduardo.Bautista@ars.usda.gov NR 33 TC 0 Z9 0 U1 4 U2 4 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9437 EI 1943-4774 J9 J IRRIG DRAIN ENG JI J. Irrig. Drainage Eng-ASCE PD DEC PY 2016 VL 142 IS 12 AR 06016012 DI 10.1061/(ASCE)IR.1943-4774.0001090 PG 11 WC Agricultural Engineering; Engineering, Civil; Water Resources SC Agriculture; Engineering; Water Resources GA EC2BL UT WOS:000387914200015 ER PT J AU Gupta, A Simmons, W Schueneman, GT Mintz, EA AF Gupta, Anju Simmons, William Schueneman, Gregory T. Mintz, Eric A. TI Lignin-coated cellulose nanocrystals as promising nucleating agent for poly(lactic acid) SO JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY LA English DT Article DE Isothermal crystallization; Lignin-coated cellulose nanocrystals; Secondary nucleation; Poly(lactic acid) and nanocomposites ID POLY(L-LACTIC ACID); CRYSTALLIZATION KINETICS; MELTING BEHAVIOR; SPHERULITE GROWTH; SURFACE-MODIFICATION; GREEN COMPOSITES; LACTIC-ACID; NANOCOMPOSITES; NANOWHISKERS; BARRIER AB We report the effect of lignin-coated cellulose nanocrystals (L-CNCs) on the crystallization behavior of poly(lactic acid) (PLA). PLA/L-CNC nanocomposites were prepared by melt mixing, and the crystallization behavior of PLA was investigated using differential scanning calorimetry. Isothermal crystallization data were analyzed using Avrami and Lauritzen-Hoffman secondary nucleation theory, while the equilibrium melting temperature was determined using the nonlinear Hoffman-Weeks method. The lignin-coated cellulose nanocrystals acted as a nucleating agent and significantly increased the rate of crystallization and degree of crystallinity of PLA in PLA/L-CNC nanocomposites. The Avrami exponent, n, increased in the presence of L-CNCs, displaying a conversion from lamellar morphology to two-dimensional crystal growth. PLA/L-CNC nanocomposites also gave lower values of the nucleation parameters, K-g and sigma(e), due to a reduction in the activation energy for nucleation. C1 [Gupta, Anju; Simmons, William; Mintz, Eric A.] Clark Atlanta Univ, Dept Chem, 223 James Brawley Dr, Atlanta, GA 30314 USA. [Gupta, Anju; Simmons, William; Mintz, Eric A.] Clark Atlanta Univ, High Performance Polymers & Composites Ctr, 223 James Brawley Dr, Atlanta, GA 30314 USA. [Schueneman, Gregory T.] US Forest Serv, Forest Prod Lab, USDA, One Gifford Pinchot Dr, Madison, WI 53726 USA. RP Mintz, EA (reprint author), Clark Atlanta Univ, Dept Chem, 223 James Brawley Dr, Atlanta, GA 30314 USA.; Mintz, EA (reprint author), Clark Atlanta Univ, High Performance Polymers & Composites Ctr, 223 James Brawley Dr, Atlanta, GA 30314 USA. EM emintz@cau.edu FU U.S. Forest Service [11-JV-11111101-050] FX The authors would like to gratefully acknowledge partial support of this work by U.S. Forest Service Grant 11-JV-11111101-050. NR 50 TC 1 Z9 1 U1 21 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1388-6150 EI 1572-8943 J9 J THERM ANAL CALORIM JI J. Therm. Anal. Calorim. PD DEC PY 2016 VL 126 IS 3 BP 1243 EP 1251 DI 10.1007/s10973-016-5657-6 PG 9 WC Thermodynamics; Chemistry, Analytical; Chemistry, Physical SC Thermodynamics; Chemistry GA EC1GK UT WOS:000387852700024 ER PT J AU Palumbo, JD O'Keeffe, TL Fidelibus, MW AF Palumbo, J. D. O'Keeffe, T. L. Fidelibus, M. W. TI Characterization of Aspergillus section Nigri species populations in vineyard soil using droplet digital PCR SO LETTERS IN APPLIED MICROBIOLOGY LA English DT Article DE Aspergillus carbonarius; Aspergillus niger; Aspergillus tubingensis; Aspergillus welwitschiae; droplet digital PCR; fungal ecology; mycotoxins; vineyards ID REAL-TIME PCR; OCHRATOXIN-A; BLACK ASPERGILLI; CARBONARIUS; GRAPES; QUANTIFICATION; ROT; DIVERSITY; INFECTION; BACTERIA AB Identification of populations of Aspergillus section Nigri species in environmental samples using traditional methods is laborious and impractical for large numbers of samples. We developed species-specific primers and probes for quantitative droplet digital PCR (ddPCR) to improve sample throughput and simultaneously detect multiple species in each sample. The ddPCR method was used to distinguish Aspergillus niger, Aspergillus welwitschiae, Aspergillus tubingensis and Aspergillus carbonarius in mixed samples of total DNA. Relative abundance of each species measured by ddPCR agreed with input ratios of template DNAs. Soil samples were collected at six time points over two growing seasons from two raisin vineyards in Fresno County, California. Aspergillus section Nigri strains were detected in these soils in the range of 10(2)-10(5)CFUg(-1). Relative abundance of each species varied widely among samples, but in 52 of 60 samples, A.niger was the most abundant species, ranging from 38 to 88% of the total population. In combination with total plate counts, this ddPCR method provides a high-throughput method for describing population dynamics of important potential mycotoxin-producing species in environmental samples. C1 [Palumbo, J. D.; O'Keeffe, T. L.] ARS, Foodborne Toxin Detect & Prevent Res Unit, USDA, Albany, CA USA. [Fidelibus, M. W.] Univ Calif, Kearney Agr Res & Extens Ctr, Parlier, CA USA. RP Palumbo, JD (reprint author), ARS, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM jeffrey.palumbo@ars.usda.gov FU U.S. Department of Agriculture Agricultural Research Service CRIS Project [2030-42000-038-00D] FX We thank S. Cisneros, J. Osorio-Aguilar, R. Fox, M. Ok, J. Govoni and Y. Ho for sample collection and processing. This work was supported by U.S. Department of Agriculture Agricultural Research Service CRIS Project 2030-42000-038-00D. NR 26 TC 0 Z9 0 U1 4 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0266-8254 EI 1472-765X J9 LETT APPL MICROBIOL JI Lett. Appl. Microbiol. PD DEC PY 2016 VL 63 IS 6 BP 458 EP 465 DI 10.1111/lam.12667 PG 8 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA EC1PN UT WOS:000387878900010 PM 27604045 ER PT J AU Ogle, SM McCarl, BA Baker, J Del Grosso, SJ Adler, PR Paustian, K Parton, WJ AF Ogle, Stephen M. McCarl, Bruce A. Baker, Justin Del Grosso, Stephen J. Adler, Paul R. Paustian, Keith Parton, William J. TI Managing the nitrogen cycle to reduce greenhouse gas emissions from crop production and biofuel expansion SO MITIGATION AND ADAPTATION STRATEGIES FOR GLOBAL CHANGE LA English DT Article DE Soil nitrous oxide; DayCent model; FASOMGHG model; Biofuel; Policy analysis; Greenhouse gas emissions; Agricultural sector; Biofuel feedstock production; Crop production ID DAILY SOLAR-RADIATION; LAND-USE CHANGE; AGRICULTURAL SOILS; OXIDE EMISSIONS; COMPLEX TERRAIN; UNITED-STATES; N2O; MITIGATION; MANAGEMENT; DAYCENT AB Public policies are promoting biofuels as an alternative to fossil fuel consumption in order to mitigate greenhouse gas (GHG) emissions. However, the mitigation benefit can be at least partially compromised by emissions occurring during feedstock production. One of the key sources of GHG emissions from biofuel feedstock production, as well as conventional crops, is soil nitrous oxide (N2O), which is largely driven by nitrogen (N) management. Our objective was to determine how much GHG emissions could be reduced by encouraging alternative N management practices through application of nitrification inhibitors and a cap on N fertilization. We used the US Renewable Fuel Standards (RFS2) as the basis for a case study to evaluate technical and economic drivers influencing the N management mitigation strategies. We estimated soil N2O emissions using the DayCent ecosystem model and applied the US Forest and Agricultural Sector Optimization Model with Greenhouse Gases (FASOMGHG) to project GHG emissions for the agricultural sector, as influenced by biofuel scenarios and N management options. Relative to the current RSF2 policy with no N management interventions, results show decreases in N2O emissions ranging from 3 to 4 % for the agricultural sector (5.5-6.5 million metric tonnes CO2 eq. year(-1); 1 million metric tonnes is equivalent to a Teragram) in response to a cap that reduces N fertilizer application and even larger reductions with application of nitrification inhibitors, ranging from 9 to 10 % (15.5-16.6 million tonnes CO2 eq. year(-1)). The results demonstrate that climate and energy policies promoting biofuel production could consider options to manage the N cycle with alternative fertilization practices for the agricultural sector and likely enhance the mitigation of GHG emissions associated with biofuels. C1 [Ogle, Stephen M.; Paustian, Keith; Parton, William J.] Colorado State Univ, Nat Resource Ecol Lab, Campus Delivery 1499, Ft Collins, CO 80523 USA. [Ogle, Stephen M.] Colorado State Univ, Dept Ecosyst Sci & Sustainabil, Ft Collins, CO 80523 USA. [McCarl, Bruce A.] Texas A&M Univ, Dept Agr Econ, College Stn, TX 77843 USA. [Baker, Justin] RTI Int, Agr Resource & Energy, Econ & Policy Program, Res Triangle Pk, NC 27703 USA. [Del Grosso, Stephen J.] ARS, Soil Plant Nutr Res, USDA, Ft Collins, CO 80526 USA. [Adler, Paul R.] ARS, Pasture Syst & Watershed Management Res Unit, USDA, University Pk, PA 16802 USA. [Paustian, Keith] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. RP Ogle, SM (reprint author), Colorado State Univ, Nat Resource Ecol Lab, Campus Delivery 1499, Ft Collins, CO 80523 USA.; Ogle, SM (reprint author), Colorado State Univ, Dept Ecosyst Sci & Sustainabil, Ft Collins, CO 80523 USA. EM Stephen.Ogle@colostate.edu OI Ogle, Stephen/0000-0003-1899-7446 FU US Environmental Protection Agency [EP08C000106]; USDA-NIFA [2011-67003-30205] FX This research was funded by the US Environmental Protection Agency, Contract No. EP08C000106, with additional support from USDA-NIFA, Grant #2011-67003-30205. We thank Cindy Keough and Ram Gurung who performed the DayCent model simulations and developed the meta-models that were used in the FASOMGHG model analysis. NR 62 TC 1 Z9 1 U1 22 U2 22 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1381-2386 EI 1573-1596 J9 MITIG ADAPT STRAT GL JI Mitig. Adapt. Strateg. Glob. Chang. PD DEC PY 2016 VL 21 IS 8 BP 1197 EP 1212 DI 10.1007/s11027-015-9645-0 PG 16 WC Environmental Sciences SC Environmental Sciences & Ecology GA EB8BY UT WOS:000387617200003 ER PT J AU McIntosh, CA Owens, DK AF McIntosh, Cecilia A. Owens, Daniel K. TI Advances in flavonoid glycosyltransferase research: integrating recent findings with long-term citrus studies SO PHYTOCHEMISTRY REVIEWS LA English DT Article; Proceedings Paper CT PSNA Meeting CY AUG 08-12, 2015 CL IL SP PSNA DE Structure/function; Substrate specificity; Biotechnological potential; Enzymology; Protein structure analysis ID GLUCOSE ANTHOCYANIDIN 3-O-GLUCOSYLTRANSFERASE; RECOMBINANT EXPRESSION; EXOGENOUS FLAVANONES; MEDICAGO-TRUNCATULA; CRYSTAL-STRUCTURES; CLITORIA-TERNATEA; STRUCTURAL BASIS; CELL-CULTURES; NARINGIN; PARADISI AB Flavonoid glycosides are required for a number of crucial roles in planta and have the potential for development in a variety of agricultural, medicinal, and biotechnological applications. A number of recent advancements have been made in characterizing glycosyltransferases, the enzymes that are responsible for the synthesis of these important molecules. In this review, glycosyltransferases are considered with regard to biochemical properties, expression patterns, levels of enzyme activity during development, and structure/function relationships. This is presented with historical context to highlight critical findings, particularly with regard to the innovative work that has come from research on citrus species. The plant glycosyltransferase crystal structures that have been solved over the past decade, either alone or in complex with sugar donor and/or acceptor molecules, are discussed. The application of results from these structures to inform current structure/function work as well as implications and goals for future crystallography and tertiary modeling studies are considered. A thorough understanding of the properties of glycosyltransferases will be a critical step in any future biotechnological application of these enzymes in areas such as crop improvement and custom design of enzymes to produce desired compounds for nutritional and/or medicinal usage. C1 [McIntosh, Cecilia A.] East Tennessee State Univ, Dept Biol Sci, ETSU Box 70703, Johnson City, TN 37614 USA. [McIntosh, Cecilia A.] East Tennessee State Univ, Sch Grad Studies, ETSU Box 70720, Johnson City, TN 37614 USA. [Owens, Daniel K.] ARS, Nat Prod Utilizat Res Unit, USDA, University, MS 38655 USA. RP McIntosh, CA (reprint author), East Tennessee State Univ, Sch Grad Studies, ETSU Box 70720, Johnson City, TN 37614 USA. EM mcintosc@etsu.edu NR 70 TC 0 Z9 0 U1 16 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1568-7767 EI 1572-980X J9 PHYTOCHEM REV JI Phytochem. Rev. PD DEC PY 2016 VL 15 IS 6 SI SI BP 1075 EP 1091 DI 10.1007/s11101-016-9460-6 PG 17 WC Plant Sciences SC Plant Sciences GA EC1IU UT WOS:000387858900004 ER PT J AU Park, JB AF Park, Jae B. TI Javamide-I-O-methyl ester increases p53 acetylation and induces cell death via activating caspase 3/7 in monocytic THP-1 cells SO PHYTOMEDICINE LA English DT Article DE Javamide-I-O-methyl ester; Coffee; Molecular docking; Sirtuin inhibition; Caspase3/7; THP-1 cell death ID COFFEE; HEALTH; ACID AB Background: Javamide-I and-II are phenolic amide compounds found in Coffea sp. Previous study suggested that javamide-II may be a potent compound with Sirt1/2 inhibition activity. Purpose: However, the effects of javamide-I and the its O-methyl ester on Sirt inhibition, p53 acetylation and cell death have not been investigated. Methods: The isolation and synthesis of javamide-I and its O-methyl ester analogue were confirmed by NMR. Their potential effects on Sirt1/2/3, p53 acetylation and cell death were examined using sirt assay, silico analysis, Western blot, caspase 3/7 and apoptotic assay methods. Results: Javamide-I and its O-methyl ester demonstrated a similar inhibition pattern; Sirt1 (IC50 of 19 mu M) better than Sirt2 (IC50 of 104 mu M) and Sirt3 (IC50 of 160 mu M). However, javamide-I and its O-methyl ester were found to inhibit Sirt1 better than Sirt2, which is different from javamide-II able to inhibit Sirt2 stronger than Sirt1. In silico analysis, javamide-I and its O-methyl ester showed a competitive binding pattern against NAD(+), which was also supported by the kinetic analysis with K-i = 20.1 mu M for javamide-I and 19.5 mu M for the O-methyl ester. However, the O-methyl ester increased p53 acetylation better than javamide-I in monocytic THP-1 cells. Since caspase 3/7 activation is often followed by the p53 activation, we investigated their effects on caspase 3/7, and found that O-methyl ester again activated caspase 3/7 greater than javamide-I in the cells, eventually leading to apoptotic cell death. Conclusion: These data suggest that the O-methyl modification in javamide-I may play a critical role in increasing p53 acetylation, activating caspase, and eventually inducing the THP-1 cell death. Published by Elsevier GmbH. C1 [Park, Jae B.] ARS, Diet Genom & Immunol Lab, BHNRC, USDA, Bldg 307C,Rm 131, Beltsville, MD 20705 USA. RP Park, JB (reprint author), ARS, PL, BHNRC, Bldg 307 Room 313, Beltsville, MD 20705 USA. EM park@307.bhnrc.usda.gov FU USDA [1235-51,000-054-00D] FX This study was funded by USDA (project number 1235-51,000-054-00D). NR 18 TC 0 Z9 0 U1 2 U2 2 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 0944-7113 EI 1618-095X J9 PHYTOMEDICINE JI Phytomedicine PD DEC 1 PY 2016 VL 23 IS 13 BP 1647 EP 1652 DI 10.1016/j.phymed.2016.10.004 PG 6 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA EC4IC UT WOS:000388090000012 PM 27823629 ER PT J AU Beckerman, J Sehneider, W AF Beckerman, J. Sehneider, W. TI Mining the Gap: Assessing Leadership Needs to Improve 21st Century Plant Pathology SO PLANT DISEASE LA English DT Article ID WOMEN C1 [Beckerman, J.] Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA. [Sehneider, W.] ARS, USDA, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA. RP Beckerman, J (reprint author), Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA. NR 22 TC 0 Z9 0 U1 8 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD DEC PY 2016 VL 100 IS 12 BP 2349 EP 2356 DI 10.1094/PDIS-03-16-0354-FE PG 8 WC Plant Sciences SC Plant Sciences GA EB8IF UT WOS:000387634000001 ER PT J AU Yang, CY Powell, CA Duan, YP Zhang, MQ AF Yang, C. Y. Powell, C. A. Duan, Y. P. Zhang, M. Q. TI Characterization and Antibacterial Activity of Oil-In-Water Nano-Emulsion Formulation Against Candidatus Liberibacter asiaticus SO PLANT DISEASE LA English DT Article ID SAO-PAULO-STATE; REAL-TIME PCR; SPONTANEOUS EMULSIFICATION; GREENING DISEASE; DELIVERY-SYSTEMS; PARTICLE-SIZE; CITRUS; NANOEMULSIONS; NANOPARTICLES; OPTIMIZATION AB Nano-emulsion is a promising delivery system for increasing pesticide use and enhancing the therapeutic efficiency against pathogens. The pathogen Candidatus Liberibacter asiaticus (Las) that causes destructive citrus huanglongbing (HLB) resides in citrus phloem, which makes it difficult to treat with chemicals. Based on various physiochemical characteristics of oils, surfactants, and organic solvents, an oil-in-water (O/W) nano-emulsion formulation was developed and optimized to combat citrus HLB. The nano-emulsion was formulated through a spontaneous emulsification method for efficient delivery of ampicillin into the citrus phloem using bark application. The nano-emulsion that was prepared from Cremophor EL (viscous oil), acetone (water miscibility organic solvent), and Span 80/Tween 80 (surfactant) formed a small droplet size (17.33 +/- 0.52 nm) and exhibited an improved absorption rate. Peak concentration was detected at 2 days posttreatment and the maximum concentration (C-max) and relative bioavailability (RBA) of ampicillin in HLB-affected citrus were 71.86 +/- 35.38 ng/g and 267.25% +/- 44.1%, respectively. The peak concentration of Amp appeared at 6 days posttreatment in the citrus trees that were treated with Amp alone and their Cmax and RBA were 56.44 +/- 32.59 ng/g and 100%, respectively. The same nano:emulsion was used to deliver five different antimicrobials to control citrus HLB through bark application. We found that the droplet size of the antimicrobials in the nano-emulsion was significantly reduced and the nano-emulsion also enhanced the therapeutic efficiency of validoxylamine A alone and in combination with actidione as well as sulfadimoethoxine sodium against Las. Therefore, this study provides an efficient bark application nano-emulsion formulation for citrus HLB control. C1 [Yang, C. Y.; Zhang, M. Q.] Guangxi Univ, State Key Lab Conversat & Utilizat Subtrop Arobio, Nanning 530005, Guangxi, Peoples R China. [Yang, C. Y.; Powell, C. A.; Zhang, M. Q.] Univ Florida, IRREC IFAS, Ft Pierce, FL 34945 USA. [Yang, C. Y.] Fujian Agr & Forestry Univ, Fuzhou 350002, Peoples R China. [Duan, Y. P.; Zhang, M. Q.] USDA ARS, USHRL, Ft Pierce, FL 34945 USA. RP Zhang, MQ (reprint author), Guangxi Univ, State Key Lab Conversat & Utilizat Subtrop Arobio, Nanning 530005, Guangxi, Peoples R China.; Zhang, MQ (reprint author), Univ Florida, IRREC IFAS, Ft Pierce, FL 34945 USA.; Zhang, MQ (reprint author), USDA ARS, USHRL, Ft Pierce, FL 34945 USA. EM mqzhang@ufl.edu FU U.S. Citrus Research and Development Foundation [584]; Guangxi Natural Science Foundation [2013GXNSFFA118002] FX We greatly appreciate Bioscience Editing Solutions for critically reading this paper and providing helpful suggestions. Financial support was provided by U.S. Citrus Research and Development Foundation (project CRDF#584) and Guangxi Natural Science Foundation (project 2013GXNSFFA118002). NR 46 TC 0 Z9 0 U1 10 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD DEC PY 2016 VL 100 IS 12 BP 2448 EP 2454 DI 10.1094/PDIS-05-16-0600-RE PG 7 WC Plant Sciences SC Plant Sciences GA EB8IF UT WOS:000387634000014 ER PT J AU Zhang, XQ Chen, MH Liang, YJ Xing, YX Yang, LT Chen, MH Comstock, JC Li, YR Yang, LT AF Zhang, Xiaoqiu Chen, Minghui Liang, Yongjian Xing, Yongxiu Yang, Litao Chen, Minghui Comstock, Jack C. Li, Yangrui Yang, Litao TI Morphological and Physiological Responses of Sugarcane to Leifsonia xyli subsp xyli Infection SO PLANT DISEASE LA English DT Article ID RATOON STUNTING DISEASE; CAUSAL BACTERIUM; GENOME SEQUENCE; GROWTH; RICE; BIOSYNTHESIS; GIBBERELLIN; METABOLISM; BINDING; STRESS AB Ratoon stunt, caused by the bacterium Leifsonia xyli subsp. xyli, is one of the major sugarcane diseases worldwide. The objectives of this study were to determine the variation in morphology and DNA sequence of L. xyli subsp. xyli strains isolated in China, to compare the changes that occurred in vascular ultrastructure and levels of endogenous hormone abscisic acid (ABA), auxins (indoleacetic acid [IAA]), and gibberellic acids (GA(3)) in sugarcane stalks Experiments were also conducted with two sugarcane varieties, 'ROC22' and 'Badila', in the greenhouse to understand the cytological and physiological mechanisms of L. xyli subsp. xyli induced growth stunting. There were three treatments in the experiments: (i) healthy plants (L. xyli subsp. xyli free plants), (ii) infected plants (L. xyli subsp. xyli infected seedcanes treated with hot water, and (iii) infected plants (healthy seedcanes dipped in L. xyli subsp. xyli cell culture). The results showed that sequence coverage of a locally isolated strain, LxxGXBZ01, was 99.99%, and the average nucleotide identity between LxxGXBZ01 and the other well-characterized Brazilian isolate LxxCTCB07 was 93.61%. LxxGXBZ01 occurred in different sizes and shapes in xylem vessels of infected plants. In comparison with healthy stalks, the secondary walls of the vessel element in L. xyli subsp. xyli infected stalks were degraded with uneven wall thickness, deformities, sticky substances, and electron-dense substances accumulated inside the cells. Compared with the healthy and hot-water treatments, the contents of IAA and GA(3) were significantly lower, while that of ABA was significantly higher in the L. xyli subsp. xyli infected stalks. The information obtained in this study will expand our understanding of ratoon stunt etiology and cytological and physiological bases of the disease manifestation. C1 [Zhang, Xiaoqiu; Chen, Minghui; Liang, Yongjian; Xing, Yongxiu; Yang, Litao] Guangxi Univ, Coll Agr, State Key Lab Subtrop Bioresources Conservat & Ut, Nanning 530005, Peoples R China. [Chen, Minghui] Ping Ding Shan Univ, Pingdingshan 46700, Henan, Peoples R China. [Comstock, Jack C.] USDA ARS, Sugarcane Field Stn, Canal Point, FL USA. [Li, Yangrui; Yang, Litao] Chinese Acad Agr Sci, Guangxi Key Lab Sugarcane Genet Improvement, Key Lab Sugarcane Biotechnol & Genet Improvement, Minist Agr,Sugarcane Res Ctr, Nanning 530007, Peoples R China. [Li, Yangrui; Yang, Litao] Guangxi Acad Agr Sci, Sugarcane Res Inst, Nanning 530007, Peoples R China. RP Yang, LT (reprint author), Guangxi Univ, Coll Agr, State Key Lab Subtrop Bioresources Conservat & Ut, Nanning 530005, Peoples R China.; Yang, LT; Li, YR (reprint author), Chinese Acad Agr Sci, Guangxi Key Lab Sugarcane Genet Improvement, Key Lab Sugarcane Biotechnol & Genet Improvement, Minist Agr,Sugarcane Res Ctr, Nanning 530007, Peoples R China.; Yang, LT; Li, YR (reprint author), Guangxi Acad Agr Sci, Sugarcane Res Inst, Nanning 530007, Peoples R China. EM litao61@hotmail.com; liyr@gxaas.net FU Natural Science Fund [31360293]; Guangxi Natural Science Fund [2012GXNSFDA053011]; International Cooperation Program Project of China [2013DFA31600]; China 863 Program [2013AA102604]; Guangxi Special Funds for Bagui Scholars and Distinguished Experts and Guangxi R D Program [GKG1222009-1B, GXN14121008-2-1] FX The authors thank R. Kumar Singh for reviewing the manuscript and H. Li for technical assistance. This work was supported by Natural Science Fund (31360293), Guangxi Natural Science Fund (2012GXNSFDA053011), International Cooperation Program Project of China (2013DFA31600), China 863 Program (2013AA102604), Guangxi Special Funds for Bagui Scholars and Distinguished Experts and Guangxi R & D Program Funding (GKG1222009-1B, GXN14121008-2-1). NR 49 TC 0 Z9 0 U1 10 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD DEC PY 2016 VL 100 IS 12 BP 2499 EP 2506 DI 10.1094/PDIS-10-15-1134-RE PG 8 WC Plant Sciences SC Plant Sciences GA EB8IF UT WOS:000387634000020 ER PT J AU Byamukama, E Tande, C Olson, J Hesler, L Grady, K Nleya, T Mathew, F AF Byamukama, E. Tande, C. Olson, J. Hesler, L. Grady, K. Nleya, T. Mathew, F. TI First Detection of Aster Yellows Associated With Phytoplasma on Camelina sativa in South Dakota SO PLANT DISEASE LA English DT News Item C1 [Byamukama, E.; Tande, C.; Grady, K.; Nleya, T.; Mathew, F.] South Dakota State Univ, Dept Agron Hort & Plant Sci, Brookings, SD 57007 USA. [Olson, J.] Oklahoma State Univ, Dept Entomol & Plant Pathol, Stillwater, OK 74078 USA. [Hesler, L.] USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. RP Byamukama, E (reprint author), South Dakota State Univ, Dept Agron Hort & Plant Sci, Brookings, SD 57007 USA. NR 4 TC 0 Z9 0 U1 4 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD DEC PY 2016 VL 100 IS 12 BP 2523 EP 2523 DI 10.1094/PDIS-10-15-1179-PDN PG 1 WC Plant Sciences SC Plant Sciences GA EB8IF UT WOS:000387634000032 ER PT J AU Stewart, JE Otto, K Cline, GA Dumroese, RK Klopfenstein, NB Kim, MS AF Stewart, J. E. Otto, K. Cline, G. A. Dumroese, R. K. Klopfenstein, N. B. Kim, M. -S. TI First Report of Fusarium proliferatum Causing Fusarium Root Disease on Sugar Pine (Pinus lambertiana) in a Forest Container Nursery in California SO PLANT DISEASE LA English DT News Item C1 [Stewart, J. E.; Otto, K.] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. [Cline, G. A.] Eldorado Natl Forest, Placerville Nursery, Camino, CA 95709 USA. [Dumroese, R. K.; Klopfenstein, N. B.] US Forest Serv, USDA, Rocky Mt Res Stn, Moscow, ID 83843 USA. [Kim, M. -S.] Kookmin Univ, Dept Forestry Environm & Syst, Seoul 02707, South Korea. RP Stewart, JE (reprint author), Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. NR 5 TC 0 Z9 0 U1 3 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD DEC PY 2016 VL 100 IS 12 BP 2534 EP 2534 DI 10.1094/PDIS-06-16-0909-PDN PG 1 WC Plant Sciences SC Plant Sciences GA EB8IF UT WOS:000387634000060 ER PT J AU Yan, GP Plaisance, A Huang, D Handoo, ZA AF Yan, G. P. Plaisance, A. Huang, D. Handoo, Z. A. TI First Report of the Lance Nematode Hoplolaimus stephanus From a Soybean Field in North Dakota SO PLANT DISEASE LA English DT News Item C1 [Yan, G. P.; Plaisance, A.; Huang, D.] North Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Handoo, Z. A.] USDA ARS, Nematol Lab, Beltsville, MD 20705 USA. RP Yan, GP (reprint author), North Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. NR 5 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD DEC PY 2016 VL 100 IS 12 BP 2536 EP 2536 DI 10.1094/PDIS-07-16-1012-PDN PG 1 WC Plant Sciences SC Plant Sciences GA EB8IF UT WOS:000387634000065 ER PT J AU Webb, SE Badillo-Vargas, IE Purcifull, DE Hiebert, E Baker, CA Funderburk, JE Adkins, S AF Webb, S. E. Badillo-Vargas, I. E. Purcifull, D. E. Hiebert, E. Baker, C. A. Funderburk, J. E. Adkins, S. TI Zucchini tigre mosaic virus Infection of Cucurbits in Florida SO PLANT DISEASE LA English DT News Item ID PAPAYA RINGSPOT VIRUS C1 [Webb, S. E.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Badillo-Vargas, I. E.] Texas A&M AgriLife Res, Weslaco, TX 78596 USA. [Purcifull, D. E.; Hiebert, E.] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA. [Baker, C. A.] FDACS DPI, Gainesville, FL 32608 USA. [Funderburk, J. E.] Univ Florida, NFREC, Quincy, FL 67891 USA. [Adkins, S.] USDA ARS, Ft Pierce, FL 34945 USA. RP Webb, SE (reprint author), Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. NR 5 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD DEC PY 2016 VL 100 IS 12 BP 2540 EP 2540 DI 10.1094/PDIS-05-16-0660-PDN PG 1 WC Plant Sciences SC Plant Sciences GA EB8IF UT WOS:000387634000074 ER PT J AU Anderson, RL AF Anderson, Randy L. TI Increasing corn yield with no-till cropping systems: a case study in South Dakota SO RENEWABLE AGRICULTURE AND FOOD SYSTEMS LA English DT Article DE conservation agriculture; crop diversity; management tactics; soil microbial community; reduced inputs ID ARBUSCULAR MYCORRHIZAL FUNGI; SOIL-MICROORGANISMS; PLANT-GROWTH; AGROECOSYSTEMS; CONSERVATION; PRODUCTIVITY; AGRICULTURE; MANAGEMENT; EFFICIENCY; ROTATION AB No-till practices have improved crop yields in the semiarid Great Plains. However, a recent assessment of research studies across the globe indicated that crop yields are often reduced by no-till. To understand this contrast, we examined corn yields across time in a no-till cropping system of one producer in central South Dakota to identify factors associated with increased yield. The producer started no-till in 1990; by 2013, corn yield increased 116%. In comparison, corn increased only 32% during this interval with a conventional, tillage-based system in a neighboring county. With no-till, corn yields increased in increments due to changes in management. For example, corn yield increased 52% when crop diversity in the rotation was expanded from 2 to 5 crops. A further 18% gain in yield occurred when dry pea was grown before corn in sequence. Nitrogen (N) requirement for corn is 25% lower in no-till compared with a tillage-based rotation. Furthermore, phosphorus (P) fertilizer input also has been reduced 30% after 20 yr of no-till, even with higher yields. Our case study shows that integrating no-till with crop diversity and soil microbial changes improves corn yield considerably. This integration also reduces need for inputs such as water, N and P. C1 [Anderson, Randy L.] USDA ARS, Brookings, SD 57006 USA. RP Anderson, RL (reprint author), USDA ARS, Brookings, SD 57006 USA. EM randy.anderson@ars.usda.gov NR 45 TC 0 Z9 0 U1 13 U2 13 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1742-1705 EI 1742-1713 J9 RENEW AGR FOOD SYST JI Renew. Agr. Food Syst. PD DEC PY 2016 VL 31 IS 6 BP 568 EP 573 DI 10.1017/S1742170515000435 PG 6 WC Agriculture, Multidisciplinary SC Agriculture GA EC1IJ UT WOS:000387857800008 ER PT J AU Turner, PA Griffis, TJ Mulla, DJ Baker, JM Venterea, RT AF Turner, P. A. Griffis, T. J. Mulla, D. J. Baker, J. M. Venterea, R. T. TI A geostatistical approach to identify and mitigate agricultural nitrous oxide emission hotspots SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Nitrous oxide; Greenhouse gas management; Biogeochemical hotspots; LiDAR digital elevation model; Geospatial cokriging ID LIDAR ELEVATION DATA; SPATIAL VARIABILITY; CHAMBER MEASUREMENTS; EDDY COVARIANCE; SOIL; FLUXES; DENITRIFICATION; FIELD; CORN; GRASSLAND AB Anthropogenic emissions of nitrous oxide (N2O), a trace gas with severe environmental costs, are greatest from agricultural soils amended with nitrogen (N) fertilizer. However, accurate N2O emission estimates at fine spatial scales are made difficult by their high variability, which represents a critical challenge for the management of N2O emissions. Here, static chamber measurements (n = 60) and soil samples (n = 129) were collected at approximately weekly intervals (n = 6) for 42-d immediately following the application of N in a southern Minnesota cornfield (15.6-ha), typical of the systems prevalent throughout the U.S. Corn Belt. These data were integrated into a geostatistical model that resolved N2O emissions at a high spatial resolution (1-m). Field-scale N2O emissions exhibited a high degree of spatial variability, and were partitioned into three classes of emission strength: hotspots, intermediate, and coldspots. Rates of emission from hotspots were 2-fold greater than non-hotspot locations. Consequently, 36% of the field-scale emissions could be attributed to hotspots, despite representing only 21% of the total field area. Variations in elevation caused hotspots to develop in predictable locations, which were prone to nutrient and moisture accumulation caused by terrain focusing. Because these features are relatively static, our data and analyses indicate that targeted management of hotspots could efficiently reduce field-scale emissions by as much 17%, a significant benefit considering the deleterious effects of atmospheric N2O. (C) 2016 Elsevier B.V. All rights reserved. C1 [Turner, P. A.; Griffis, T. J.; Mulla, D. J.; Baker, J. M.; Venterea, R. T.] Univ Minnesota, Dept Soil Water & Climate, 439 Borlaug Hall,1991 Upper Buford Circle, St Paul, MN 55108 USA. [Turner, P. A.; Griffis, T. J.; Mulla, D. J.; Baker, J. M.; Venterea, R. T.] USDA ARS, Soil & Water Management Unit, 1991 Upper Buford Circle, St Paul, MN 55108 USA. RP Turner, PA (reprint author), Univ Minnesota, Dept Soil Water & Climate, 439 Borlaug Hall,1991 Upper Buford Circle, St Paul, MN 55108 USA.; Turner, PA (reprint author), USDA ARS, Soil & Water Management Unit, 1991 Upper Buford Circle, St Paul, MN 55108 USA. EM turne289@umn.edu FU U.S. Department of Agriculture (USDA) [USDA-NIFA 2013-67019-21364]; USDA - Agricultural Research Service FX We thank Jeff Wood, Matt Erickson, Mike Dolan, William Breiter, Ke Xiao, Zichong Chen, and Lucas Rosen for field and laboratory assistance. This work was supported by the U.S. Department of Agriculture (USDA) Grant USDA-NIFA 2013-67019-21364 and the USDA - Agricultural Research Service. We are also appreciative of the private landowner who volunteered their field for our use. NR 48 TC 0 Z9 0 U1 12 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD DEC 1 PY 2016 VL 572 BP 442 EP 449 DI 10.1016/j.scitotenv.2016.08.094 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA EC0RA UT WOS:000387807200045 PM 27543947 ER PT J AU Bytnerowicz, A Hsu, YM Percy, K Legge, A Fenn, ME Schilling, S Fraczek, W Alexander, D AF Bytnerowicz, Andrzej Hsu, Yu-Mei Percy, Kevin Legge, Allan Fenn, Mark E. Schilling, Susan Fraczek, Witold Alexander, Diane TI Ground-level air pollution changes during a boreal wildland mega-fire SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Fine particulate matter; Reactive nitrogenous species; Sulfur dioxide; Sulfate; Ozone ID ATHABASCA OIL SANDS; PASSIVE SAMPLING SYSTEM; AMBIENT NITRIC-ACID; NITROUS-ACID; TROPOSPHERIC OZONE; CLIMATE-CHANGE; TRACE GASES; ATMOSPHERIC DEPOSITION; CHEMICAL-COMPOSITION; AMMONIA EMISSIONS AB The 2011 Richardson wildland mega-fire in the Athabasca Oil Sands Region (AOSR) in northern Alberta, Canada had large effects on air quality. At a receptor site in the center of the AOSR ambient PM2.5, O-3, NO, NO2, SO2, NH3, HONO, HNO3, NH4+ and NO3- were measured during the April-August 2011 period. Concentrations of NH3, HNO3, NO2, SO2 and O-3 were also monitored across the AOSR with passive samplers, providing monthly summer and bimonthly winter average values in 2010, 2011 and 2012. During the fire, hourly PM2.5 concentrations >450 mu g m(-3) were measured at the AMS 1 receptor site. The 24-h National Ambient Air Quality Standard (NAAQS) of 35 mu g m(-3) and the Canada Wide Standard (CWS) of 30 mu g m(-3) were exceeded on 13 days in May and 7 days in June. During the fire emission periods, sharp increases in NH3, HONO, HNO3, NH4+, NO3- and total inorganic reactive N concentrations occurred, all closely correlated with the PM2.5 changes. There were large differences in the relative contribution of various N compounds to total inorganic N between the no-fire emission and fire emission periods. While in the absence of fires NO and NO2 dominated, their relative contribution during the fires was similar to 2 fold smaller, mainly due to increased NH3, NH4+ and NO3-. Concentrations of HONO and HNO3 also greatly increased during the fires, but their contribution to the total inorganic N pool was relatively small. Elevated NH3 and HNO3 concentrations affected large areas of northern Alberta during the Richardson Fire. While NH3 and HNO3 concentrations were not at levels considered toxic to plants, these gases contributed significantly to atmospheric N deposition. Generally, no significant changes in O-3 and SO2 concentrations were detected and their ambient concentrations were below levels harmful to human health or sensitive vegetation. Published by Elsevier B.V. C1 [Bytnerowicz, Andrzej; Fenn, Mark E.; Schilling, Susan; Alexander, Diane] USDA, Forest Serv, Pacific Southwest Res Stn, 4955 Canyon Crest Dr, Riverside, CA 92507 USA. [Hsu, Yu-Mei; Percy, Kevin] Wood Buffalo Environm Assoc, 100-330 Thickwood Blvd, Ft McMurray, AB T9K 1Y1, Canada. [Legge, Allan] Biosphere Solut, Calgary, AB T2N 1H7, Canada. [Fraczek, Witold] Environm Syst Res Inst, Redlands, CA 92373 USA. RP Bytnerowicz, A (reprint author), USDA, Forest Serv, Pacific Southwest Res Stn, 4955 Canyon Crest Dr, Riverside, CA 92507 USA. EM abytnerowicz@fs.fed.us; mfenn@fs.fed.us FU Wood Buffalo Environmental Association (WBEA), Fort McMurray, Alberta, Canada [NRMG-11-CO-11272139-025, 06-CO-11272172-005] FX This work was funded by the Wood Buffalo Environmental Association (WBEA), Fort McMurray, Alberta, Canada (Agreement numbers NRMG-11-CO-11272139-025 and 06-CO-11272172-005). The authors thank WBEA members and staff for assistance with field monitoring activities and other means of support for this study. Comments from Drs. Owen Cooper and Meiyun Lin on early versions of the manuscript are greatly appreciated. The content and opinions expressed by the authors in this article do not necessarily reflect the views of the WBEA and its members. The authors gratefully acknowledge the NOAA Air Resources Laboratory (ARL) for the provision of the HYSPLIT transport and dispersion model and/or READY website (http://www.ready.noaa.gov) used in this publication. NR 67 TC 0 Z9 0 U1 27 U2 27 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD DEC 1 PY 2016 VL 572 BP 755 EP 769 DI 10.1016/j.scitotenv.2016.07.052 PG 15 WC Environmental Sciences SC Environmental Sciences & Ecology GA EC0RA UT WOS:000387807200073 PM 27622696 ER PT J AU Carim, KJ Eby, LA Barfoot, CA Boyer, MC AF Carim, Kellie J. Eby, Lisa A. Barfoot, Craig A. Boyer, Matthew C. TI Consistent loss of genetic diversity in isolated cutthroat trout populations independent of habitat size and quality SO CONSERVATION GENETICS LA English DT Article DE Cutthroat trout; Isolation management; 50/500 rule; Genetic drift; Habitat fragmentation ID CONTEMPORARY EFFECTIVE POPULATION; CHARR SALVELINUS-LEUCOMAENIS; ONCORHYNCHUS-CLARKII-LEWISI; CROSS-SPECIES AMPLIFICATION; LINKAGE DISEQUILIBRIUM; HEADWATER STREAMS; CONSERVATION STRATEGY; LOCAL ADAPTATION; PACIFIC SALMON; BULL TROUT AB Fragmentation and isolation of wildlife populations has reduced genetic diversity worldwide, leaving many populations vulnerable to inbreeding depression and local extinction. Nonetheless, isolation is protecting many native aquatic species from interactions with invasive species, often making reconnection an unrealistic conservation strategy. Isolation management is widely used to protect extant cutthroat trout (Oncorhynchus clarkii) populations from invasive species. Despite this, few studies have empirically examined how predictor variables including habitat length, population size, time since isolation and habitat quality, relate to levels of genetic diversity in isolated trout populations. We compared allelic richness of cutthroat trout across 14 microsatellite loci in two connected and 12 anthropogenically isolated populations of the Flathead River basin, Montana. Isolated populations in habitat fragments < 8 km stream length had reduced genetic diversity, but diversity was not significantly related to any of our predictor variables. To broaden our scope, we analyzed seven geologically isolated populations from the same river basin occupying habitat fragments up to 18 km in length. These populations showed reduced diversity, regardless of fragment size. Furthermore, geologically isolated populations had significantly lower average allelic richness compared to streams recently isolated by anthropogenic activities. These results demonstrate a consistent loss of genetic diversity through time in isolated populations, emphasizing the need to explore strategies to minimize risks of inbreeding depression. Testing conservation theory and subsequent assumptions broadly across taxa is necessary to ensure efficacy of conservation efforts. C1 [Carim, Kellie J.] US Forest Serv, Rocky Mt Res Stn, Natl Genom Ctr Wildlife & Fish Conservat, 800 E Beckwith Ave, Missoula, MT 59801 USA. [Carim, Kellie J.; Eby, Lisa A.] Univ Montana, Wildlife Biol Program, 32 Campus Dr, Missoula, MT 59812 USA. [Barfoot, Craig A.] Confederated Salish & Kootenai Tribes, POB 278, Pablo, MT 59855 USA. [Carim, Kellie J.; Boyer, Matthew C.] Montana Fish Wildlife & Pk, 490 North Meridian Rd, Kalispell, MT 59901 USA. RP Carim, KJ (reprint author), US Forest Serv, Rocky Mt Res Stn, Natl Genom Ctr Wildlife & Fish Conservat, 800 E Beckwith Ave, Missoula, MT 59801 USA.; Carim, KJ (reprint author), Univ Montana, Wildlife Biol Program, 32 Campus Dr, Missoula, MT 59812 USA.; Carim, KJ (reprint author), Montana Fish Wildlife & Pk, 490 North Meridian Rd, Kalispell, MT 59901 USA. EM kelliejcarim@fs.fed.us FU Westslope Chapter of Trout Unlimited; P.E.O. Scholars Award; Confederated Salish and Kootenai Tribes; National Science Foundation Graduate Research Fellowship [DGE-0809127]; Bonneville Power Administration [199101903] FX We thank W. Lowe, D. Patterson, C. Muhlfeld, M. Schwartz, and three anonymous reviewers for feedback on previous versions of this work. We also acknowledge our funding sources for this study, including the Westslope Chapter of Trout Unlimited, P.E.O. Scholars Award, the Confederated Salish and Kootenai Tribes, National Science Foundation Graduate Research Fellowship Program DGE-0809127, and Bonneville Power Administration Grant#199101903 to Montana Fish, Wildlife, and Parks. We would like to thank the many people that aided in data collection and processing of genetic samples, including the field crews with the Confederated Salish and Kootenai Tribes and Montana Fish, Wildlife, and Parks, and Sally Painter, Angela Lodmell and Steve Amish with the University of Montana Conservation Genetics Lab. Finally, we thank Fred Allendorf for guidance with data analysis and interpretation. NR 84 TC 0 Z9 0 U1 23 U2 23 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1566-0621 EI 1572-9737 J9 CONSERV GENET JI Conserv. Genet. PD DEC PY 2016 VL 17 IS 6 BP 1363 EP 1376 DI 10.1007/s10592-016-0867-9 PG 14 WC Biodiversity Conservation; Genetics & Heredity SC Biodiversity & Conservation; Genetics & Heredity GA EB2WB UT WOS:000387222200011 ER PT J AU Cross, TB Naugle, DE Carlson, JC Schwartz, MK AF Cross, Todd B. Naugle, David E. Carlson, John C. Schwartz, Michael K. TI Hierarchical population structure in greater sage-grouse provides insight into management boundary delineation SO CONSERVATION GENETICS LA English DT Article DE Centrocercus urophasianus; Microsatellites; Non-invasive sampling; Genetic diversity; Spatial principal components analysis ID MULTILOCUS GENOTYPE DATA; CENTROCERCUS-UROPHASIANUS; MICROSATELLITE LOCI; ENERGY DEVELOPMENT; HABITAT SELECTION; GENETIC DIVERSITY; TETRAO-TETRIX; NORTH-AMERICA; SAGEBRUSH HABITATS; PACIFIC-NORTHWEST AB Understanding population structure is important for guiding ongoing conservation and restoration efforts. The greater sage-grouse (Centrocercus urophasianus) is a species of concern distributed across 1.2 million km(2) of western North America. We genotyped 1499 greater sage-grouse from 297 leks across Montana, North Dakota and South Dakota using a 15 locus microsatellite panel, then examined spatial autocorrelation, spatial principal components analysis, and hierarchical Bayesian clustering to identify population structure. Our results show that at distances of up to similar to 240 km individuals exhibit greater genetic similarity than expected by chance, suggesting that the cumulative effect of short-range dispersal translates to long-range connectivity. We found two levels of hierarchical genetic subpopulation structure. These subpopulations occupy significantly different elevations and are surrounded by divergent vegetative communities with different dominant subspecies of sagebrush, each with its own chemical defense against herbivory. We propose five management groups reflective of genetic subpopulation structure. These genetic groups are largely synonymous with existing priority areas for conservation. On average, 85.8 % of individuals within each conservation priority area assign to a distinct subpopulation. Our results largely support existing management decisions regarding subpopulation boundaries. C1 [Cross, Todd B.; Schwartz, Michael K.] US Forest Serv, USDA, Natl Genom Ctr Wildlife & Fish Conservat, Rocky Mt Res Stn, 800 East Beckwith Ave, Missoula, MT 59801 USA. [Cross, Todd B.; Naugle, David E.] Univ Montana, Coll Forestry & Conservat, 32 Campus Dr, Missoula, MT 59812 USA. [Carlson, John C.] Montana State Off, Bur Land Management, 5001 Southgate Dr, Billings, MT 59101 USA. RP Cross, TB (reprint author), US Forest Serv, USDA, Natl Genom Ctr Wildlife & Fish Conservat, Rocky Mt Res Stn, 800 East Beckwith Ave, Missoula, MT 59801 USA.; Cross, TB (reprint author), Univ Montana, Coll Forestry & Conservat, 32 Campus Dr, Missoula, MT 59812 USA. EM tbcross@fs.fed.us FU Montana and Dakotas Bureau of Land Management [07-IA-11221643-343, 10-IA-11221635-027, 14-IA-11221635-059]; Great Northern Landscape Conservation Cooperative [12-IA-11221635-132]; Natural Resources Conservation Service-Sage Grouse Initiative [13-IA-11221635-054] FX This study was supported by grants from the Montana and Dakotas Bureau of Land Management (07-IA-11221643-343, 10-IA-11221635-027, 14-IA-11221635-059), the Great Northern Landscape Conservation Cooperative (12-IA-11221635-132), and the Natural Resources Conservation Service-Sage Grouse Initiative (13-IA-11221635-054). NR 112 TC 1 Z9 1 U1 13 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1566-0621 EI 1572-9737 J9 CONSERV GENET JI Conserv. Genet. PD DEC PY 2016 VL 17 IS 6 BP 1417 EP 1433 DI 10.1007/s10592-016-0872-z PG 17 WC Biodiversity Conservation; Genetics & Heredity SC Biodiversity & Conservation; Genetics & Heredity GA EB2WB UT WOS:000387222200015 ER PT J AU Hu, YH Chen, X Feng, XJ Woeste, KE Zhao, P AF Hu, Yiheng Chen, Xi Feng, Xiaojia Woeste, Keith E. Zhao, Peng TI Characterization of the complete chloroplast genome of the endangered species Carya sinensis (Juglandaceae) SO CONSERVATION GENETICS RESOURCES LA English DT Article DE Chinese hickory; Annamocarya; Conservation; Phylogeny; Endemic; Red list ID SEQUENCE DATA; ANGIOSPERMS AB Carya sinensis (Chinese Hickory, beaked walnut, or beaked hickory) is an endangered species that needs urgent conservation action. Here, we reported the complete chloroplast (cp) genome sequence and the genomic features of the C. sinensis cp, which is the first complete cp genome of any member of Carya. The C. sinensis cp genome was 160,195 bp, containing a large single copy region (LSC) of 89,541 bp and a small single copy region (SSC) of 18,538 bp separated by a pair of 26,058 bp inverted repeat regions (IRs). The genome contained 135 genes, including 87 protein-coding genes (79 species), 40tRNA genes (30tRNA species) and eight ribosomal RNA genes (four rRNA species). Most genes occurred as a single copy; 22 were duplicated. The overall GC content of the C. sinensis cp genome was 36.3 %; the corresponding GC content values of the LSC, SSC and IR regions were 33.9, 30.0 and 42.5 %, respectively. A maximum likelihood (ML) phylogenetic analysis showed that C. sinensis is closely related to Juglans, as expected based on taxonomic affinity. The complete cp genome of C. sinensis will be useful for further investigations of genetic diversity and conservation of this endemic relict woody plant. C1 [Hu, Yiheng; Chen, Xi; Feng, Xiaojia; Zhao, Peng] Northwest Univ, Coll Life Sci, Minist Educ, Key Lab Resource Biol & Biotechnol Western China, Taibaibei Rd 229, Xian 710069, Shaanxi Provinc, Peoples R China. [Woeste, Keith E.] Purdue Univ, Dept Forestry & Nat Resources, US Forest Serv, USDA,HTIRC, 715 West State St, W Lafayette, IN 47907 USA. RP Zhao, P (reprint author), Northwest Univ, Coll Life Sci, Minist Educ, Key Lab Resource Biol & Biotechnol Western China, Taibaibei Rd 229, Xian 710069, Shaanxi Provinc, Peoples R China. EM berlin@stumail.nwu.edu.cn; 781291157@qq.com; 332146704@qq.com; kwoeste@fs.fed.us; pengzhao@nwu.edu.cn FU National Natural Science Foundation of China [41471038, 31200500, J1210063]; Program for Excellent Young Academic Backbones - Northwest University, Changjiang Scholars and Innovative Research Team in University [IRT1174] FX This work was supported by the National Natural Science Foundation of China (No. 41471038, No. 31200500 and No. J1210063), the Program for Excellent Young Academic Backbones funding by Northwest University, Changjiang Scholars and Innovative Research Team in University (IRT1174). Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that also may be suitable. NR 15 TC 0 Z9 0 U1 14 U2 14 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1877-7252 EI 1877-7260 J9 CONSERV GENET RESOUR JI Conserv. Genet. Resour. PD DEC PY 2016 VL 8 IS 4 BP 467 EP 470 DI 10.1007/s12686-016-0601-4 PG 4 WC Biodiversity Conservation; Genetics & Heredity SC Biodiversity & Conservation; Genetics & Heredity GA EB6HN UT WOS:000387482700025 ER PT J AU Ki, SJ Park, J Park, Y Jeon, DJ Kim, JH AF Ki, Seo Jin Park, Jihwan Park, Yongeun Jeon, Dong Jin Kim, Joon Ha TI Developing statistical models for estimating chlorophyll-a and total suspended solid levels at an estuarine reservoir with nutrient inputs from satellite observations SO DESALINATION AND WATER TREATMENT LA English DT Article DE Bio-optical algorithms; Geostationary Ocean Color Imager; Moderate Resolution Imaging Spectroradiometer; Multiple linear regression; CE-QUAL-W2 model; Reservoir water quality ID OCEAN COLOR; COASTAL WATERS; SENSITIVITY-ANALYSIS; PARTICULATE MATTER; REMOTE ESTIMATION; INLAND WATERS; FRESH-WATER; ALGORITHMS; SEAWIFS; REFLECTANCE AB Providing high-resolution monitoring data is essential in promoting decision-making activities for surface water quality protection such as scientific modeling and data analysis. In this study, statistical models using multiple linear regression (MLR) were developed to estimate Chlorophyll-a (Chl-a) and total suspended solid (TSS) concentrations in a mesotrophic reservoir, the Yeongam Reservoir in Korea, from satellite observations. Two types of satellite data that covered different spectral regions, for wavelengths in 412-865nm (for the Geostationary Ocean Color Imager) and those in 405-14,385nm (for the Moderate Resolution Imaging Spectroradiometer), were used as inputs for statistical models, after bias correction. The MLR models for Chl-a and TSS were initially constructed and evaluated with 39 image data-sets obtained during 2011-2014. Subsequently, they compared with their corresponding algorithms that were developed under different environmental settings as well as the CE-QUAL-W2 model, a numerical model of reservoir water quality. Sensitivity analysis showed that specific red and near-infrared wavelengths significantly contributed to improve the accuracy of the Chl-a and TSS estimates, respectively, along with those of blue and green bands typically used. The constructed MLR models showed better performance than the simulation model as well as the classical and recent bio-optical algorithms on average. In particular, poor prediction performance for total nitrogen and total phosphorus caused by a lack of adequate input data and description of transport mechanisms appeared to lower the accuracy of the Chl-a and TSS estimates in the simulation model. Therefore, these results demonstrate that statistical models developed from satellite observations can be used to rapidly screen local water quality and to provide high-quality data for reservoir water quality management. C1 [Ki, Seo Jin; Jeon, Dong Jin; Kim, Joon Ha] GIST, Sch Environm Sci & Engn, Gwangju 61005, South Korea. [Park, Jihwan] KERC, Res & Dev Grp, Gangnamdae Ro 262, Seoul 06265, South Korea. [Park, Yongeun] USDA ARS, Environm Microbial & Food Safety Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Kim, JH (reprint author), GIST, Sch Environm Sci & Engn, Gwangju 61005, South Korea. EM joonkim@gist.ac.kr OI Park, Yongeun/0000-0002-1959-0843 FU Korea Ministry of Environment as "The Eco-innovation Project: Non-point source pollution control research group"; National Research Foundation of Korea FX This research was supported by the Korea Ministry of Environment as "The Eco-innovation Project: Non-point source pollution control research group" and the National Research Foundation of Korea. NR 50 TC 0 Z9 0 U1 4 U2 4 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 1944-3994 EI 1944-3986 J9 DESALIN WATER TREAT JI Desalin. Water Treat. PD DEC PY 2016 VL 57 IS 57 BP 27587 EP 27600 DI 10.1080/19443994.2016.1175772 PG 14 WC Engineering, Chemical; Water Resources SC Engineering; Water Resources GA EB1EJ UT WOS:000387092100015 ER PT J AU Collins, BM Lydersen, JM Fry, DL Wilkin, K Moody, T Stephens, SL AF Collins, Brandon M. Lydersen, Jamie M. Fry, Danny L. Wilkin, Katherine Moody, Tadashi Stephens, Scott L. TI Variability in vegetation and surface fuels across mixed-conifer-dominated landscapes with over 40 years of natural fire SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Heterogeneity; Forest restoration; Fire regime; Managed fire; Reference conditions ID NORTHERN SIERRA-NEVADA; YOSEMITE-NATIONAL-PARK; GROWTH JEFFREY PINE; LAKE TAHOE BASIN; FOREST STRUCTURE; SPATIAL-PATTERNS; PRESCRIBED FIRE; PONDEROSA PINE; SEVERITY FIRE; RESTORATION TREATMENTS AB Studies of historical fire and vegetation conditions in dry conifer forests have demonstrated a high degree of heterogeneity across landscapes. However, there is a limit to the amount of inference that can be drawn from historical fire reconstructions. Contemporary "reference" landscapes may be able to provide information that is not available from historical reconstructions. In this study, we characterized variability in vegetation structure and composition across two Sierra Nevada landscapes with long-established fire restoration programs. We used tree, shrub, and surface fuel data from 117 initial plots, 86 of which were re-measured 8-12 years later, to identify the mechanisms driving variability in vegetation and fuel conditions. Our analyses identified nine distinct vegetation groups, with mean live tree basal area and density ranging from 0.3 to 72.7 m(2) ha(-1) and 2.5 to 620 trees ha(-1) for individual groups. For all plots combined, mean live tree basal area and density was 28.4 m2 ha(-1) and 215 trees ha(-1), but standard deviations (SD) were 29.1 m2 ha(-1) and 182 trees ha(-1), respectively. These ranges and SDs demonstrate considerable variability in vegetation structure, which was partially related to site productivity and previous fire severity. Fine surface fuel loads were generally low (overall mean, 16.1 Mg ha(-1)), but also exhibited high variability (SD, 12.6 Mg ha(-1)). Surprisingly, surface fuel loads based on initial measurement and change between measurements were not related to fire characteristics. The only statistical relationship found was that surface fuel loads were associated with forest structure and composition. These results capture a contemporary 'natural' range of variability and can be used to guide landscape-level restoration efforts. More specifically, these results can help identify distinct targets for variable forest structures across landscapes. Published by Elsevier B.V. C1 [Collins, Brandon M.; Lydersen, Jamie M.] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95618 USA. [Collins, Brandon M.] Univ Calif Berkeley, Ctr Fire Res & Outreach, Berkeley, CA 94720 USA. [Fry, Danny L.; Wilkin, Katherine; Moody, Tadashi; Stephens, Scott L.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Ecosyst Sci Div, Berkeley, CA 94720 USA. [Moody, Tadashi] Calif Dept Forestry & Fire Protect, Fire & Resource Assessment Program, Sacramento, CA 94244 USA. RP Collins, BM (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95618 USA. EM bcollins@berkeley.edu FU USDA/USDI Joint Fire Sciences Program FX We sincerely thank the follow people for their assistance collecting field data: Christal Niederer, Zev Balsen, Gary Roller, Chris Dow, Keith Arnold, Shane Fairchild, Amy Foo, Carolyn Richter, and Megan Seymour. Shane Fairchild helped with data compilation and error checking. Jay Miller provided much of the fire severity data used in our analysis. This project was funded by the USDA/USDI Joint Fire Sciences Program. We thank the staff at Yosemite and Sequoia-Kings Canyon National Parks for their continued support of this research. NR 86 TC 1 Z9 1 U1 15 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD DEC 1 PY 2016 VL 381 BP 74 EP 83 DI 10.1016/j.foreco.2016.09.010 PG 10 WC Forestry SC Forestry GA EB6VC UT WOS:000387522700007 ER PT J AU Clark, SL Schweitzer, CJ AF Clark, Stacy L. Schweitzer, Callie J. TI Stand dynamics of an oak woodland forest and effects of a restoration treatment on forest health SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Oak woodland; Forest restoration; Silviculture; Cumberland Plateau; Stand dynamics; Tree-ring growth ID EASTERN NORTH-AMERICA; FUEL REDUCTION TREATMENTS; TREE GROWTH-RESPONSE; CANOPY DISTURBANCE; HARDWOOD FOREST; PRESCRIBED FIRE; UNITED-STATES; HISTORICAL DEVELOPMENT; CUMBERLAND PLATEAU; TEMPERATE FOREST AB Woodland restoration has been conducted in many countries, primarily in Mediterranean regions, but has only recently been attempted on publically and privately owned lands in the eastern United States. We reconstructed historical stand dynamics and tested the immediate effects of an oak (Quercus) woodland restoration treatment on forest health, inferred from tree-ring widths (TRW). The stands were upland white oak (Q. alba) and chestnut oak (Q. prinus) dominated and were located on the Cumberland Plateau of eastern Kentucky, USA. The stands regenerated primarily under a severe disturbance regime concurrent with peak industrial logging approximately 100 years ago. A relatively high percentage of trees (38 percent) recruited under large canopy gaps or clearings, indicative of a severe disturbance; however, gap-phase dynamics was also an important process in oak recruitment to the canopy. Primarily small (<31 cm DBH) and young (<110 years old) trees were removed during the restoration treatment, and mean DBH of residual trees was 13 cm larger than harvested trees. Residual trees were 22 years older than harvested trees, but this difference was not significant. The largest and oldest trees represented important legacy trees that could provide desirable forest biodiversity attributes. Residual trees had larger TRWs than harvested trees, beginning in the 1930s, and these differences increased over time. Residual trees also had larger TRW during two recent drought events (1986 and 1999), but recovery following drought was similar between residual and harvested trees. Managers can use well established silvicultural techniques to obtain desired stand structural conditions, while selecting healthy trees that have better response to stress factors such as drought. The oak woodland restoration treatment may help to maintain residual overstory trees until oak regeneration can be recruited to provide sustainability towards the next generation. Published by Elsevier B.V. C1 [Clark, Stacy L.] US Forest Serv, USDA, Southern Res Stn, 2431 Joe Johnson Dr, Knoxville, TN 37919 USA. [Schweitzer, Callie J.] US Forest Serv, USDA, Southern Res Stn, Huntsville, AL USA. RP Clark, SL (reprint author), US Forest Serv, USDA, Southern Res Stn, 2431 Joe Johnson Dr, Knoxville, TN 37919 USA. EM stacyclark@fs.fed.us; cschweitzer@fs.fed.us NR 65 TC 0 Z9 0 U1 26 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD DEC 1 PY 2016 VL 381 BP 258 EP 267 DI 10.1016/j.foreco.2016.09.026 PG 10 WC Forestry SC Forestry GA EB6VC UT WOS:000387522700024 ER PT J AU Cox, LE Hart, JL Dey, DC Schweitzer, CJ AF Cox, Lauren E. Hart, Justin L. Dey, Daniel C. Schweitzer, Callie J. TI Composition, structure, and intra-stand spatial patterns along a disturbance severity gradient in a Quercus stand SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Canopy disturbance; Quercus (oak); Stand development; Succession ID EASTERN NORTH-AMERICA; SCALE WIND DISTURBANCE; CENTRAL NEW-ENGLAND; LAND-USE HISTORY; CUMBERLAND PLATEAU; NATURAL DISTURBANCE; FOREST STRUCTURE; OAK FORESTS; ACCELERATED SUCCESSION; CATASTROPHIC WIND AB Natural forest disturbances, which drive succession and development, differ in extent, severity, and return interval and range from frequent, gap-scale disturbances, to infrequent stand-replacing events. Most studies have focused on natural disturbances near the ends of the disturbance severity gradient and relatively little quantitative information is available on intermediate-severity disturbance. On 20 April 2011, an EF1 tornado tracked 5 km through the Sipsey Wilderness in Alabama and resulted in a patchwork mosaic of disturbed areas. To analyze the effects of the intermediate-severity wind event on composition, structure, and intra-stand spatial patterns, we established a 100 x 200 m (2 ha) rectangular plot perpendicular to the path of the storm within an affected Quercus alba stand. Based on the basal area removed (i.e. basal area of snags, snapped stems, or uprooted stems in decay class 1) by the wind event, we divided the plot into disturbance classes (minimal, light, and moderate) to compare compositional and structural attributes along a disturbance severity gradient. Composition varied little across the disturbance gradient, but diversity was highest in the moderately disturbed neighborhoods. Stems were relatively intermingled by species (i.e. each tree neighbored by trees of different species) in each disturbance severity class. However, some species, such as Fagus grandifolia and Ostrya virginiana exhibited less intermingling than Quercus spp. and stems classed in the "other spp." taxonomic group. Large stems were disproportionately removed by the storm in the light and moderate disturbance categories. In the light disturbance class, O. virginiana was significantly less likely to experience mortality from the storm, which may in part explain the relatively high density of O. virginiana stems in the plot. (C) 2016 Elsevier B.V. All rights reserved. C1 [Cox, Lauren E.] Univ Calif Berkeley, Coll Nat Resources, Berkeley, CA 94720 USA. [Hart, Justin L.] Univ Alabama, Dept Geog, Tuscaloosa, AL 35487 USA. [Dey, Daniel C.] US Forest Serv, USDA, Northern Res Stn, Columbia, MO 65211 USA. [Schweitzer, Callie J.] US Forest Serv, USDA, Southern Res Stn, Huntsville, AL 35801 USA. RP Cox, LE (reprint author), Univ Calif Berkeley, Coll Nat Resources, Berkeley, CA 94720 USA. EM lecox@berkeley.edu FU Northern Research Station, USDA Forest Service; University of Alabama FX This project was funded through a Joint Venture Agreement between the Northern Research Station, USDA Forest Service and the University of Alabama. We thank Win Cowden, Amanda Keasberry, and Cindy Taylor for assistance in the field, the Bank head National Forest staff for logistical support, and Megan Buchanan and Michael Steinberg for comments on prior drafts of the manuscript. We are grateful for comments provided two anonymous reviewers that greatly improved the manuscript. NR 96 TC 0 Z9 0 U1 21 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD DEC 1 PY 2016 VL 381 BP 305 EP 317 DI 10.1016/j.foreco.2016.09.040 PG 13 WC Forestry SC Forestry GA EB6VC UT WOS:000387522700029 ER PT J AU Li, XH Liu, YH Jia, Q LaMacchia, V O'Donoghue, K Huang, ZY AF Li, Xianhua Liu, Yanhong Jia, Qian LaMacchia, Virginia O'Donoghue, Kathryn Huang, Zuyi TI A systems biology approach to investigate the antimicrobial activity of oleuropein SO JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY LA English DT Article ID GENOME-SCALE RECONSTRUCTION; STAPHYLOCOCCUS-AUREUS; PHENOLIC-COMPOUNDS; ESCHERICHIA-COLI; METABOLIC NETWORK; TRANSPORTER PROP; MODEL MEMBRANES; OLEA-EUROPAEA; DEHYDROGENASE; HYDROLYSIS AB Oleuropein and its hydrolysis products are olive phenolic compounds that have antimicrobial effects on a variety of pathogens, with the potential to be utilized in food and pharmaceutical products. While the existing research is mainly focused on individual genes or enzymes that are regulated by oleuropein for antimicrobial activities, little work has been done to integrate intracellular genes, enzymes and metabolic reactions for a systematic investigation of antimicrobial mechanism of oleuropein. In this study, the first genome-scale modeling method was developed to predict the system-level changes of intracellular metabolism triggered by oleuropein in Staphylococcus aureus, a common food-borne pathogen. To simulate the antimicrobial effect, an existing S. aureus genome-scale metabolic model was extended by adding the missing nitric oxide reactions, and exchange rates of potassium, phosphate and glutamate were adjusted in the model as suggested by previous research to mimic the stress imposed by oleuropein on S. aureus. The developed modeling approach was able to match S. aureus growth rates with experimental data for five oleuropein concentrations. The reactions with large flux change were identified and the enzymes of fifteen of these reactions were validated by existing research for their important roles in oleuropein metabolism. When compared with experimental data, the up/down gene regulations of 80% of these enzymes were correctly predicted by our modeling approach. This study indicates that the genome-scale modeling approach provides a promising avenue for revealing the intracellular metabolism of oleuropein antimicrobial properties. C1 [Li, Xianhua; LaMacchia, Virginia; O'Donoghue, Kathryn; Huang, Zuyi] Villanova Univ, Dept Chem Engn, Villanova, PA 19085 USA. [Liu, Yanhong] ARS, Mol Characterizat Foodborne Pathogens, Eastern Reg Res Ctr, USDA, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. [Jia, Qian] Rowan Univ, Dept Hlth & Exercise Sci, Glassboro, NJ USA. RP Huang, ZY (reprint author), Villanova Univ, Dept Chem Engn, Villanova, PA 19085 USA. EM zuyi.huang@villanova.edu FU Villanova VCASE Seed Grant FX Funding was provided by Villanova VCASE Seed Grant. NR 42 TC 1 Z9 1 U1 4 U2 4 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1367-5435 EI 1476-5535 J9 J IND MICROBIOL BIOT JI J. Ind. Microbiol. Biotechnol. PD DEC PY 2016 VL 43 IS 12 BP 1705 EP 1717 DI 10.1007/s10295-016-1841-8 PG 13 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA EB7UY UT WOS:000387597600008 PM 27771782 ER PT J AU DaVanon, KA Howard, LK Mabry, KE Schooley, RL Bestelmeyer, BT AF DaVanon, Kristen A. Howard, Lindsey K. Mabry, Karen E. Schooley, Robert L. Bestelmeyer, Brandon T. TI Effects of exurban development on trophic interactions in a desert landscape SO LANDSCAPE ECOLOGY LA English DT Article DE Chihuahuan Desert; Coyote; Lagomorph; Rodent; Plant recruitment; Urbanization ID CHIHUAHUAN DESERT; BOTTOM-UP; TOP-DOWN; LAND-USE; LONG-TERM; URBAN; COMMUNITIES; PATTERNS; COYOTE; URBANIZATION AB Mechanisms of ecosystem change in urbanizing landscapes are poorly understood, especially in exurban areas featuring residential or commercial development set in a matrix of modified and natural vegetation. We asked how development altered trophic interactions and ecosystem processes in the matrix. We examined the effect of varying degrees of exurban development (housing density) on a trophic system that included an apex mammalian predator (coyote, Canis latrans), mammalian herbivores (lagomorphs and rodents), and herbaceous plants. We tested the hypothesis that plant recruitment would be negatively affected by exurban development due either to increases in herbivores associated with increased resource availability (a bottom-up effect) or to a reduction in predators that avoid humans (a top-down effect). In Las Cruces, New Mexico, USA, four replicate sites were located in each of three urbanization levels: high density exurban, low density exurban, and wildland dominated by Chihuahuan Desert vegetation. Seedling trays measured herbivory rates, live trapping estimated abundance of pocket mice and kangaroo rats, and remotely-triggered wildlife cameras estimated the activity of lagomorphs and coyotes. Increased herbivory on seedlings and decreased herbaceous plant recruitment were observed in high density exurban areas. Overall rodent abundance, seed consumption rates, and activity of the lagomorph Lepus californicus did not vary with urbanization level. Activity by another lagomorph, Sylvilagus audubonii, and coyotes was highest in dense exurban areas, consistent with a bottom-up effect. Exurban development can have important indirect effects on trophic interactions occurring in adjacent, untransformed ecosystems. Similar to earlier studies, such effects in the Chihuahuan Desert may be mediated by bottom-up processes associated with anthropogenic inputs. C1 [DaVanon, Kristen A.; Howard, Lindsey K.; Mabry, Karen E.] New Mexico State Univ, Dept Biol, Las Cruces, NM 88003 USA. [Schooley, Robert L.] Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA. [Bestelmeyer, Brandon T.] ARS, Jornada Expt Range, USDA, Las Cruces, NM 88003 USA. RP Bestelmeyer, BT (reprint author), ARS, Jornada Expt Range, USDA, Las Cruces, NM 88003 USA. EM bbestelm@nmsu.edu FU National Science Foundation through the Jornada Basin LTER site [DEB0080412]; TE, Inc FX This study was funded by the National Science Foundation through the Jornada Basin LTER site (DEB0080412) and by T&E, Inc. We thank William Gould and Darren James for statistical help and Mark Andersen and Donovan Bailey for suggestions and comments on earlier versions of this work. We also thank Maria Chavez for help in the field. Finally, we thank the Bureau of Land Management for coordinating use of the wildland sites and several Las Cruces residents for allowing us the use of their yards. NR 57 TC 0 Z9 0 U1 25 U2 25 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD DEC PY 2016 VL 31 IS 10 BP 2343 EP 2354 DI 10.1007/s10980-016-0403-9 PG 12 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA EB4GS UT WOS:000387329100010 ER PT J AU Tucker, MM Corace, RG Cleland, DT Kashian, DM AF Tucker, Madelyn M. Corace, R. Gregory, III Cleland, David T. Kashian, Daniel M. TI Long-term effects of managing for an endangered songbird on the heterogeneity of a fire-prone landscape SO LANDSCAPE ECOLOGY LA English DT Article DE Jack pine; Historic range of variability; Pre-European settlement; Public land surveys; Northern Lower Michigan; Kirtland's warbler ID NORTHERN LOWER MICHIGAN; PINE-DOMINATED ECOSYSTEMS; PUBLIC LAND SURVEY; JACK PINE; KIRTLANDS WARBLER; SPECIES ACT; SPATIAL-PATTERNS; FOREST; MANAGEMENT; VARIABILITY AB Jack pine (Pinus banksiana)-dominated ecosystems of northern Lower Michigan are the primary breeding habitat for the federally endangered Kirtland's warbler (Setophaga kirtlandii, KW). Historically, young stands used by KW were produced by stand-replacing wildfires, but fire suppression has necessitated the management of jack pine plantations for KW habitat since the 1970s. Effects of this long-term management on landscape age heterogeneity have previously not been quantified. We hypothesized that forest management has altered the spatial and temporal distribution of jack pine-dominated ecosystems beyond their historic range of variability. By developing a diameter-age relationship for jack pine, we estimated ages of pre-European settlement trees found in General Land Office survey notes. We compared pre-European and current landscapes using geostatistical modeling of survey notes, and landscape metrics to quantify changes in pattern. Three KW management-based age classes (< 20, 21-50, > 50 years) are now more evenly distributed (31, 39, and 30 %, respectively) compared to the pre-European distribution (5, 19, 76 %) with little variability over time. Landscape metrics suggest the current landscape is younger and more fragmented than the pre-European landscape. These changes indicate restriction of the historic range of age variability, largely due to conversion of older jack pine stands to young KW habitat plantations. Management has met KW population objectives, but has altered the temporal variability of the landscape's age structure. Pre-European settlement patterns of stand-ages may provide a foundation for an ecosystem-based management plan for the region that supports both KW and the ecosystems upon which they depend. C1 [Tucker, Madelyn M.; Kashian, Daniel M.] Wayne State Univ, Dept Biol Sci, Biol Sci Bldg,5047 Gullen Mall, Detroit, MI 48202 USA. [Corace, R. Gregory, III] USDI Fish & Wildlife Serv, Seney Natl Wildlife Refuge, 1674 Refuge Entrance Rd, Seney, MI 49883 USA. [Cleland, David T.] US Forest Serv, Vegetat Ecol Detached, USDA, Washington, DC USA. RP Kashian, DM (reprint author), Wayne State Univ, Dept Biol Sci, Biol Sci Bldg,5047 Gullen Mall, Detroit, MI 48202 USA. EM dkash@wayne.edu FU Joint Fire Science Program FX We wish to acknowledge the assistance of several people instrumental in procuring data for this research: T. Greco, Michigan Department of Natural Resources; P. Huber and L. McNichols, US Forest Service; D. Rothstein, Michigan State University; and E. Schools, Michigan Natural Features Inventory. We also thank the Kirtland's Warbler Recovery Team for helpful feedback. T. Dowling, D.R. Kashian, D. Rothstein, and two anonymous reviewers made helpful comments on earlier versions of this manuscript, and P. Huber and S. Sjogren provided constructive feedback in the earliest stages of this study. This project was funded with a grant from the Joint Fire Science Program to R.G.C. III and D.M.K. The findings and conclusions of this article are those of the authors and do not necessarily represent the views of the U. S. Fish and Wildlife Service or other agencies and institutions. NR 59 TC 0 Z9 0 U1 14 U2 14 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD DEC PY 2016 VL 31 IS 10 BP 2445 EP 2458 DI 10.1007/s10980-016-0413-7 PG 14 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA EB4GS UT WOS:000387329100017 ER PT J AU Bunce, JA AF Bunce, J. A. TI Light dependence of carboxylation capacity for C-3 photosynthesis models SO PHOTOSYNTHETICA LA English DT Article DE carbon dioxide; climate change; modeling; temperature ID RIBULOSE-BISPHOSPHATE CARBOXYLASE; MESOPHYLL CONDUCTANCE; CO2 CONCENTRATION; TEMPERATURE RESPONSE; ELECTRON-TRANSPORT; IN-VIVO; LEAVES; ACTIVATION; RUBISCO; IMPACT AB C-3 photosynthesis at high light is often modeled by assuming limitation by the maximum capacity of Rubisco carboxylation (V-Cmax) at low CO2 concentrations, by electron transport capacity (J(max)) at higher CO2 concentrations, and sometimes by triose-phosphate utilization rate at the highest CO2 concentrations. Net photosynthetic rate (P (N)) at lower light is often modeled simply by assuming that it becomes limited by electron transport (J). However, it is known that Rubisco can become deactivated at less than saturating light, and it is possible that P (N) at low light could be limited by the rate of Rubisco carboxylation (V (C)) rather than J. This could have important consequences for responses of P (N) to CO2 and temperature at low light. In this work, P (N) responses to CO2 concentration of common bean, quinoa, and soybean leaves measured over a wide range of temperatures and PPFDs were compared with rates modeled assuming either VC or J limitation at limiting light. In all cases, observed rates of P (N) were better predicted by assuming limitation by VC rather than J at limiting light both below and above the current ambient CO2. One manifestation of this plant response was that the relative stimulation of P (N) with increasing the ambient CO2 concentration from 380 to 570 A mu mol mol(-1) did not decrease at less than saturating PPFDs. The ratio of VC to V-Cmax at each lower PPFD varied linearly with the ratio of P (N) at low PPFD to P (N) at high PPFD measured at 380 A mu mol(CO2) mol(-1) in all cases. This modification of the standard C-3 biochemical model was much better at reproducing observed responses of light-limited P (N) to CO2 concentrations from pre-industrial to projected future atmospheric concentrations. C1 [Bunce, J. A.] ARS, USDA, Crop Syst & Global Change Lab, BARC West, 10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Bunce, JA (reprint author), ARS, USDA, Crop Syst & Global Change Lab, BARC West, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM James.Bunce@ars.usda.gov NR 21 TC 0 Z9 0 U1 15 U2 15 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0300-3604 EI 1573-9058 J9 PHOTOSYNTHETICA JI Photosynthetica PD DEC PY 2016 VL 54 IS 4 BP 484 EP 490 DI 10.1007/s11099-016-0215-9 PG 7 WC Plant Sciences SC Plant Sciences GA EB1EC UT WOS:000387090500002 ER PT J AU Pettigrew, WT AF Pettigrew, W. T. TI Cultivar variation in cotton photosynthetic performance under different temperature regimes SO PHOTOSYNTHETICA LA English DT Article DE abiotic stress; gas exchange; maximum quantum yield; thermotolerance; water-use efficiency ID GOSSYPIUM-HIRSUTUM; GENOTYPIC DIFFERENCES; HEAT-STRESS; CO2; RESPIRATION; ACTIVATION; RUBISCO; RATES AB Cotton (Gossypium hirsutum L.) yields are impacted by overall photosynthetic production. Factors that influence crop photosynthesis are the plants genetic makeup and the environmental conditions. This study investigated cultivar variation in photosynthesis in the field conditions under both ambient and higher temperature. Six diverse cotton cultivars were grown in the field at Stoneville, MS under both an ambient and a high temperature regime during the 2006-2008 growing seasons. Mid-season leaf net photosynthetic rates (P (N)) and dark-adapted chlorophyll fluorescence variable to maximal ratios (F-v/F-m) were determined on two leaves per plot. Temperature regimes did not have a significant effect on either P (N) or F-v/F-m. In 2006, however, there was a significant cultivar x temperature interaction for P (N) caused by PeeDee 3 having a lower P (N) under the high temperature regime. Other cultivars' P (N) were not affected by temperature. FM 800BR cultivar consistently had a higher P (N) across the years of the study. Despite demonstrating a higher leaf F-v/F-m, ST 5599BR exhibited a lower P (N) than the other cultivars. Although genetic variability was detected in photosynthesis and heat tolerance, the differences found were probably too small and inconsistent to be useful for a breeding program. C1 [Pettigrew, W. T.] ARS, USDA, Crop Prod Syst Res Unit, POB 350,141 Expt Stn Rd, Stoneville, MS 38776 USA. RP Pettigrew, WT (reprint author), ARS, USDA, Crop Prod Syst Res Unit, POB 350,141 Expt Stn Rd, Stoneville, MS 38776 USA. EM bill.pettigrew@ars.usda.gov NR 27 TC 0 Z9 0 U1 12 U2 12 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0300-3604 EI 1573-9058 J9 PHOTOSYNTHETICA JI Photosynthetica PD DEC PY 2016 VL 54 IS 4 BP 502 EP 507 DI 10.1007/s11099-016-0208-8 PG 6 WC Plant Sciences SC Plant Sciences GA EB1EC UT WOS:000387090500004 ER PT J AU Chiang, KS Bock, CH Lee, IH El Jarroudi, M Delfosse, P AF Chiang, Kuo-Szu Bock, Clive H. Lee, I-Hsuan El Jarroudi, Moussa Delfosse, Philippe TI Plant Disease Severity Assessment-How Rater Bias, Assessment Method, and Experimental Design Affect Hypothesis Testing and Resource Use Efficiency SO PHYTOPATHOLOGY LA English DT Article ID HORSFALL-BARRATT SCALE; IMAGE-ANALYSIS; VISUAL ESTIMATION; CITRUS CANKER; RELIABILITY; ACCURACY; PRECISION; SYMPTOMS; INTERVAL; LEAVES AB The effect of rater bias and assessment method on hypothesis testing was studied for representative experimental designs for plant disease assessment using balanced and unbalanced data sets. Data sets with the same number of replicate estimates for each of two treatments are termed "balanced" and those with unequal numbers of replicate estimates are termed "unbalanced". The three assessment methods considered were nearest percent estimates (NPEs), an amended 10% incremental scale, and the Horsfall-Barratt (H-B) scale. Estimates of severity of Septoria leaf blotch on leaves of winter wheat were used to develop distributions for a simulation model. The experimental designs are presented here in the context of simulation experiments which consider the optimal design for the number of specimens (individual units sampled) and the number of replicate estimates per specimen for a fixed total number of observations (total sample size for the treatments being compared). The criterion used to gauge each method was the power of the hypothesis test. As expected, at a given fixed number of observations, the balanced experimental designs invariably resulted in a higher power compared with the unbalanced designs at different disease severity means, mean differences, and variances. Based on these results, with unbiased estimates using NPE, the recommended number of replicate estimates taken per specimen is 2 (from a sample of specimens of at least 30), because this conserves resources. Furthermore, for biased estimates, an apparent difference in the power of the hypothesis test was observed between assessment methods and between experimental designs. Results indicated that, regardless of experimental design or rater bias, an amended 10% incremental scale has slightly less power compared with NPEs, and that the H-B scale is more likely than the others to cause a type II error. These results suggest that choice of assessment method, optimizing sample number and number of replicate estimates, and using a balanced experimental design are important criteria to consider to maximize the power of hypothesis tests for comparing treatments using disease severity estimates. C1 [Chiang, Kuo-Szu; Lee, I-Hsuan] Natl Chung Hsing Univ, Dept Agron, Div Biometr, Taichung 40201, Taiwan. [Bock, Clive H.] ARS, USDA, Southeastern Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. [El Jarroudi, Moussa] Univ Liege, Dept Environm Sci & Management, 185 Ave Longwy, B-6700 Arlon, Belgium. [Delfosse, Philippe] Luxembourg Inst Sci & Technol, 41 Rue Brill, L-4422 Belvaux, Luxembourg. RP Bock, CH (reprint author), ARS, USDA, Southeastern Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. EM clive.bock@ars.usda.gov FU Ministry of Science and Technology of Taiwan, R.O.C. [MOST 104-2313-B-005-027, MOST 105-2313-B-005-013]; United States Department of Agriculture (USDA) Agricultural Research Service [6042-21220-012-00]; Administration des Services Techniques de l'Agriculture, Luxembourg FX We thank F. van den Bosch, S. Parnell, and T. Gottwald for many discussions on aspects of plant disease assessment that provided a basis for developing this work. K.-S. Chiang gratefully acknowledges financial support from the Ministry of Science and Technology of Taiwan, R.O.C. (MOST 104-2313-B-005-027 and MOST 105-2313-B-005-013). C. Bock acknowledges support for the research through the United States Department of Agriculture (USDA) Agricultural Research Service project number 6042-21220-012-00. M. El Jarroudi and P. Delfosse thank the Administration des Services Techniques de l'Agriculture, Luxembourg for funding the MACRY project. This article reports the results of research only. Mention of a trademark or proprietary product is solely for the purpose of providing specific information and does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products that may also be suitable. NR 36 TC 0 Z9 0 U1 6 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD DEC PY 2016 VL 106 IS 12 BP 1451 EP 1464 DI 10.1094/PHYTO-12-15-0315-R PG 14 WC Plant Sciences SC Plant Sciences GA EB6AW UT WOS:000387464100002 PM 27532427 ER PT J AU Longland, WS Dimitri, LA AF Longland, William S. Dimitri, Lindsay A. TI Can seed caching enhance seedling survival of Indian ricegrass (Achnatherum hymenoides) through intraspecific facilitation? SO PLANT ECOLOGY LA English DT Article DE Heteromyid rodents; Scatterhoarding; Seedling recruitment; Survival analysis ID POSITIVE INTERACTIONS; ORYZOPSIS-HYMENOIDES; DISPERSAL; RODENTS; ESTABLISHMENT; BITTERBRUSH; COMMUNITIES; GERMINATION; DENSITY; PLANT AB Positive interactions among individual plants (facilitation) may often enhance seedling survival in stressful environments. Many granivorous small mammal species cache groups of seeds for future consumption in shallowly buried scatterhoards, and seeds of many plant species germinate and establish aggregated clusters of seedlings from such caches. Scatterhoards made by desert heteromyid rodents provide a major source of seedling recruitment for Indian ricegrass (Achnatherum hymenoides), a perennial bunchgrass species occurring widely across arid southwestern deserts of North America. However, effects of the resulting clumping of seedlings on subsequent survival have not been quantified under field conditions, which include extended periods of excessive summer heat and little precipitation. We monitored Indian ricegrass seedlings transplanted into field exclosures at two western Nevada study sites as either single seedlings or clumps of 25 or 35 seedlings and compared their survival. Survival was positively correlated with the number of seedlings growing together, and survival of whole clumps of 25 or 35 seedlings was significantly greater than that of seedlings growing singly. Moreover, individual seedlings within clumps of 35 seedlings had significantly higher survival than seedlings growing singly. No single seedlings survived through the hot and very dry summer following their planting, but a small proportion (1.8-2.8 %) of individual seedlings within clumps survived. Results of this field experiment suggest that facilitative benefits accruing to Indian ricegrass due to seed-caching desert rodents can extend past seedling establishment and into the long-term survival of the plant. C1 [Longland, William S.; Dimitri, Lindsay A.] USDA ARS, Great Basin Rangelands Res Unit, 920 Valley Rd, Reno, NV 89512 USA. RP Longland, WS (reprint author), USDA ARS, Great Basin Rangelands Res Unit, 920 Valley Rd, Reno, NV 89512 USA. EM bill.longland@ars.usda.gov NR 23 TC 0 Z9 0 U1 4 U2 4 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-0237 EI 1573-5052 J9 PLANT ECOL JI Plant Ecol. PD DEC PY 2016 VL 217 IS 12 BP 1523 EP 1532 DI 10.1007/s11258-016-0665-y PG 10 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA EB4OG UT WOS:000387350600007 ER PT J AU Gonzalez-Andujar, JL Chantre, GR Morvillo, C Blanco, AM Forcella, F AF Gonzalez-Andujar, J. L. Chantre, G. R. Morvillo, C. Blanco, A. M. Forcella, F. TI Predicting field weed emergence with empirical models and soft computing techniques SO WEED RESEARCH LA English DT Review DE artificial neural networks; genetic algorithms; predictive modelling; nonlinear regression; weed control; day degrees; d degrees C ID RAGWEED AMBROSIA-TRIFIDA; SEEDLING EMERGENCE; AVENA-FATUA; PARAMETER-ESTIMATION; GERMINATION; CORN; DYNAMICS; DORMANCY; CROPS AB Seedling emergence is one of the most important phenological processes that influence the success of weed species. Therefore, predicting weed emergence timing plays a critical role in scheduling weed management measures. Important efforts have been made in the attempt to develop models to predict seedling emergence patterns for weed species under field conditions. Empirical emergence models have been the most common tools used for this purpose. They are based mainly on the use of temperature, soil moisture and light. In this review, we present the more popular empirical models, highlight some statistical and biological limitations that could affect their predictive accuracy and, finally, we present a new generation of modelling approaches to tackle the problems of conventional empirical models, focusing mainly on soft computing techniques. We hope that this review will inspire weed modellers and that it will serve as a basis for discussion and as a frame of reference when weproceed to advance the modelling of field weed emergence. C1 [Gonzalez-Andujar, J. L.; Morvillo, C.] CSIC, Inst Agr Sostenible, Aptdo 4084, Cordoba 14080, Spain. [Chantre, G. R.] Univ Nacl Sur, Dept Agron, CONICET, CERZOS, Bahia Blanca, Buenos Aires, Argentina. [Morvillo, C.] Univ Buenos Aires, Dept Prod Vegetal, Fac Agron, Buenos Aires, DF, Argentina. [Blanco, A. M.] Univ Nacl Sur, Planta Piloto Ingn Quim, CONICET, Bahia Blanca, Buenos Aires, Argentina. [Forcella, F.] USDA ARS, North Cent Soil Conservat Res Lab, Morris, MN USA. RP Gonzalez-Andujar, JL (reprint author), CSIC, Inst Agr Sostenible, Aptdo 4084, Cordoba 14080, Spain. EM andujar@cica.es RI GONZALEZ-ANDUJAR, JOSE /G-5671-2013; OI GONZALEZ-ANDUJAR, JOSE /0000-0003-2356-4098; Chantre, Guillermo/0000-0002-4424-0204 FU FEDER (European Regional Development Funds); Spanish Ministry of Economy and Competitiveness funds [AGL2012-33736]; Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET); Universidad Nacional del Sur (PGI-UNS) FX J. L. Gonzalez-Andujar was supported by FEDER (European Regional Development Funds) and the Spanish Ministry of Economy and Competitiveness funds (Projects AGL2012-33736). G. R. Chantre was supported by grants from Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) and Universidad Nacional del Sur (PGI-UNS). NR 50 TC 1 Z9 1 U1 8 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0043-1737 EI 1365-3180 J9 WEED RES JI Weed Res. PD DEC PY 2016 VL 56 IS 6 BP 415 EP 423 DI 10.1111/wre.12223 PG 9 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA EB4RD UT WOS:000387359800002 ER PT J AU Tautges, NE Sullivan, TS Reardon, CL Burke, IC AF Tautges, Nicole E. Sullivan, Tarah S. Reardon, Catherine L. Burke, Ian C. TI Soil microbial diversity and activity linked to crop yield and quality in a dryland organic wheat production system SO APPLIED SOIL ECOLOGY LA English DT Article DE Carbon substrate utilization; Grain yield; Microbial abundance; Nitrogen uptake; Soil enzyme activity; T-RFLP ID RESTRICTION-FRAGMENT-LENGTH; ARBUSCULAR MYCORRHIZAL FUNGI; 16S RIBOSOMAL-RNA; BETA-GLUCOSAMINIDASE ACTIVITY; CONVENTIONAL FARMING SYSTEMS; SEEDED GRAIN SYSTEMS; T-RFLP DATA; ALTERNATIVE STRATEGIES; NORTHERN IDAHO; NEW-ZEALAND AB One of the primary goals of organic agriculture is increasing soil quality through the enhancement of soil biological diversity and activity. Greater soil microbial activity and diversity increase soil organic matter turnover and contribute to soil fertility, one of the main challenges associated with organic management. The objectives of this study were to 1) compare soil microbial abundance and activity between organic and conventional cropping systems, and 2) explore connections between soil microbial community indicators and crop productivity in organic and conventional winter wheat (Triticum aestivum)/spring wheat/winter pea (Pisum sativum) rotations. Soil and plant tissue was sampled following six years of organic and conventional management, and soil was analyzed for microbial abundance and activity. Fungal and bacterial abundance, soil enzyme activity, and soil organic carbon (C) were greater in the organic system than in the conventional system, and all four measures were positively correlated. Community-level physiological profiling (CLPP) indicated that C substrate utilization was greater in the organic than in the conventional system, though bacterial T-RFLP data did not demonstrate different community structure between systems, suggesting that management type affected bacterial community function, but not structure. Fungal T-RFLP results indicated that fungal community structure was different between the organic and conventional systems. Hay yield and tissue nitrogen (N) were greater in the organic system, and were positively correlated with fungal and bacterial abundance, but grain yield and protein were greater in the conventional system. The results of this study indicate that management type (organic vs. conventional) has implications for microbial abundance and microbial community function, and that differences in soil microbial abundance and activity likely impact crop yields and N uptake. (C) 2016 Elsevier B.V. All rights reserved. C1 [Tautges, Nicole E.; Sullivan, Tarah S.; Burke, Ian C.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Reardon, Catherine L.] ARS, Columbia Plateau Conservat Res Ctr, USDA, Pendleton, OR 97801 USA. RP Tautges, NE (reprint author), Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. EM nicole.tautges@wsu.edu OI Tautges, Nicole/0000-0003-1708-6966 FU USDA-NIFA Organic Agricultural Research and Extension Initiative (OREI) grant [2009-51300-05578] FX The authors would like to thank Shane Hetrick, Jeanene DeAvila, and Elizabeth Torres for their technical expertise and assistance in the lab. Also, thanks to the anonymous reviewers whose comments improved this manuscript. Funding for the project was provided by USDA-NIFA Organic Agricultural Research and Extension Initiative (OREI) grant 2009-51300-05578. NR 70 TC 0 Z9 0 U1 49 U2 49 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD DEC PY 2016 VL 108 BP 258 EP 268 DI 10.1016/j.apsoil.2016.09.003 PG 11 WC Soil Science SC Agriculture GA EA5FC UT WOS:000386643800029 ER PT J AU Cornelius, ML Dieckhoff, C Hoelmer, KA Olsen, RT Weber, DC Herlihy, MV Talamas, EJ Vinyard, BT Greenstone, MH AF Cornelius, Mary L. Dieckhoff, Christine Hoelmer, Kim A. Olsen, Richard T. Weber, Donald C. Herlihy, Megan V. Talamas, Elijah J. Vinyard, Bryan T. Greenstone, Matthew H. TI Biological control of sentinel egg masses of the exotic invasive stink bug Halyomorpha halys (Stal) in Mid-Atlantic USA ornamental landscapes SO BIOLOGICAL CONTROL LA English DT Article DE Natural enemies; Urban landscape; Egg parasitoid; Biological invasion ID TRISSOLCUS BASALIS INSECTA; MICHIGAN NATIVE PLANTS; HEMIPTERA-PENTATOMIDAE; HETEROPTERA-PENTATOMIDAE; HYMENOPTERA SCELIONIDAE; NATURAL ENEMIES; NORTH-AMERICA; PATCH DEFENSE; ABUNDANCE; PARASITOIDS AB Biological invasions have far reaching effects on native plant and arthropod communities. This study evaluated the effect of natural enemies on eggs of the exotic invasive brown marmorated stink bug Halyomorpha halys (Stal) in experimental plots comprising species pairs of 16 ornamental trees and shrub genera from either Eurasia or North America and in wooded areas adjacent to the plots. Sentinel egg masses were placed on leaves of Acer, Cercis, Hydrangea, and Prunus in the plots and in seven genera of trees and shrubs in adjacent woods. Overall, rates of parasitism and predation in experimental plots were low, accounting for only 3.8% and 4.4% of egg mortality, respectively. There were no significant differences in parasitism and predation rates between native or exotic plots or between plants of different genera. In 2015, predation was significantly higher in the experimental plots than in the wooded sites, but parasitism was significantly higher in the wooded sites. In the experimental plots, seven native and one exotic parasitoid species attacked sentinel egg masses. Six native parasitoid species attacked sentinel egg masses in the wooded sites. Parasitoids in the genus Trissolcus were more likely to attack egg masses in exotic plots than in native plots. There is no evidence that native natural enemies attacking eggs of the exotic BMSB were more prevalent in landscapes with native ornamental trees and shrubs than those with exotic trees and shrubs. Published by Elsevier Inc. C1 [Cornelius, Mary L.; Weber, Donald C.; Herlihy, Megan V.; Greenstone, Matthew H.] USDA ARS, Invas Insect Biocontrol & Behav Lab, Beltsville Agr Res Ctr, 10300 Baltimore Ave, Beltsville, MD 20705 USA. [Dieckhoff, Christine; Hoelmer, Kim A.] USDA ARS, Beneficial Insects Intro Res Lab, 501 South Chapel St, Newark, DE 19713 USA. [Olsen, Richard T.] USDA ARS, US USNA, 3501 New York Ave NE, Washington, DC 20002 USA. [Talamas, Elijah J.] USDA ARS, Systemat Entomol Lab, Natl Museum Nat Hist, Smithsonian Inst, 10th & Constitut Ave NW,MRC 168, Washington, DC 20560 USA. [Vinyard, Bryan T.] USDA ARS, Stat Grp, Northeast Area Off, 10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Cornelius, ML (reprint author), USDA ARS, Invas Insect Biocontrol & Behav Lab, Beltsville Agr Res Ctr, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM mary.cornelius@ars.usda.gov FU U.S. Department of Agriculture - National Institute of Food and Agriculture - Specialty Crop Research Initiative (USDA-NIFA-SCRI) [2011-51181-30937] FX We are indebted to Colien Hefferan and Margaret Pooler for institutional support; Scott Aker and Christopher Carley for advice on horticultural protocols; Anthony Vlhakis, Tanya Zastrow; Emily Hren, Jing Hu, and Michael Fizdale for assistance with plot maintenance; Ruby Rivera-Cruz and Rosario Vidales for field assistance during the 2014 season; and The University of Puerto Rico, Florida International University, and Friends of Agricultural Research Beltsville, for enabling the participation of Mss Rivera-Cruz and Vidales. We also thank Charley Williams for designing plots that closely simulate urban landscapes, David Kidwell-Slak for additional assistance in the field, and Jason Mottern, USDA ARS BARC Systematic Entomology Laboratory, for identification of Ooencyrtus. This work was partially supported by the U.S. Department of Agriculture - National Institute of Food and Agriculture - Specialty Crop Research Initiative (USDA-NIFA-SCRI) #2011-51181-30937. NR 48 TC 3 Z9 3 U1 30 U2 30 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD DEC PY 2016 VL 103 BP 11 EP 20 DI 10.1016/j.biocontrol.2016.07.011 PG 10 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA EA2ZZ UT WOS:000386468300002 ER PT J AU Larson, KM Duan, JJ AF Larson, Kristi M. Duan, Jian J. TI Differences in the reproductive biology and diapause of two congeneric species of egg parasitoids (Hymenoptera: Encyrtidae) from northeast Asia: Implications for biological control of the invasive emerald ash borer (Coleoptera: Buprestidae) SO BIOLOGICAL CONTROL LA English DT Article DE Invasive; Wood boring insects; Life history; Bet-hedging; Agrilus planipennis; Oobius primorskyensis; Oobius agrili ID OOBIUS-AGRILI HYMENOPTERA; PLANIPENNIS FAIRMAIRE; AMERICA; CHINA; ESTABLISHMENT; DURATION; HISTORY AB The emerald ash borer Agrilus planipennis (Coleoptera: Buprestidae) is a serious invasive pest that has killed millions of North American ash (Fraxinus spp.) trees in the United States and Canada since its first detection in 2002. Oobius primorskyensis Yao and Duan and Oobius agrili Zhang and Huang (Hymenoptera: Encyrtidae) constitute a cryptic species complex of egg parasitoids attacking A. planipennis in their native range of northeast Asia. While O. primorskyensis is currently being evaluated in quarantine for potential biocontrol introduction against A. planipennis to the U. S., O. agrili has established populations in some areas of the U.S. since its first introduction in 2007. In the present study, we quantify and compare the survivorship, fecundity and progeny diapause between O. primorskyensis and O. agrili for both diapaused and non-diapaused types. Both parasitoid species were reared in the laboratory with fresh A. planipennis eggs at 25 +/- 1 degrees C, approximate to 65% RH, and 16: 8 h L: D photoperiod. Results of the study demonstrate that there are distinctive differences in the reproductive biology and progeny diapause pattern between the two parasitoid species as well as between diapaused and non-diapaused types within each species. Adults of diapaused O. primorskyensis had significantly longer longevity and produced significantly more progeny over their lifetime than diapaused adult O. agrili. Although there was no significant difference in their longevity, adults of the non-diapaused O. priomorskyensis produced significantly less progeny than those of non-diapaused O. agrili. While a majority (> 64%) of the progeny produced by diapaused O. primorskyensis and both diapaused and non-diapaused O. agrili continued development to adults, nearly 100% of the progeny from non-diapaused O. primorskyensis entered diapause. These findings suggest that the effectiveness of O. priomorskyensis and O. agrili in classical biocontrol of A. planipennis may vary in different regions of North America, where the host phenology in adult emergence and oviposition may differ. Our study indicates that O. priomorskyensis and O. agrili may be complementary to each other in biocontrol of A. planipennis in regions where both species can be successfully established. Published by Elsevier Inc. C1 [Larson, Kristi M.; Duan, Jian J.] ARS, USDA, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. [Larson, Kristi M.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. RP Duan, JJ (reprint author), ARS, USDA, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. EM jian.duan@ars.usda.gov NR 32 TC 1 Z9 1 U1 23 U2 23 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD DEC PY 2016 VL 103 BP 39 EP 45 DI 10.1016/j.biocontrol.2016.08.001 PG 7 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA EA2ZZ UT WOS:000386468300005 ER PT J AU Castrillo, LA Griggs, MH Vandenberg, JD AF Castrillo, Louela A. Griggs, Michael H. Vandenberg, John D. TI Competition between biological control fungi and fungal symbionts of ambrosia beetles Xylosandrus crassiusculus and X. germanus (Coleoptera: Curculionidae): Mycelial interactions and impact on beetle brood production SO BIOLOGICAL CONTROL LA English DT Article DE Ambrosia fungi; Ambrosiella spp.; Entomopathogenic fungi; Beauveria bassiana; Metarhizium brunneum; Mycoparasitic fungi; Trichoderma spp.; Microbial control ID XYLEBORUS-GLABRATUS; BEAUVERIA-BASSIANA; LIFE-HISTORY; TRICHODERMA; SCOLYTIDAE; HOST; BIOCONTROL; MANAGEMENT; RAFFAELEA; MYCANGIA AB Ambrosia beetles Xylosandrus crassiusculus and X. germanus are among the most important exotic pests of orchards and nurseries in the US and are difficult to control using conventional insecticides because of their cryptic habits. The use of biological control agents may prove effective by targeting both beetles and fungal symbionts inside tree galleries: entomopathogenic fungi could be used to target beetle foundresses and their brood, or mycoparasitic fungi, e.g., Trichoderma harzianum, could be used to target their associated fungal symbionts. We used a combination of in vitro assays and beetle bioassays to examine competition between symbionts and biological control fungi and the impact of biological control fungi on beetle brood production. The in vitro assays showed T. harzianum outcompeted different strains of Ambrosiella roeperi and A. grosmanniae associated with X. crassiusculus and X. germanus, respectively, whether in primary or secondary resource capture assays. In contrast, entomopathogenic fungi Beauveria bassiana and Metarhizium brunneum blocked the spread of symbionts only in primary competition assays. Complementary beetle bioassays showed that beetle galleries in T. harzianum-treated beech stems had sparse symbiont growth, many with no or only a small number of eggs present. Brood numbers produced by foundresses in T. harzianum-treated stems were comparable to those in stems treated with either entomopathogen at the higher dosages, in which brood reduction was likely due to foundress mortality prior to laying eggs or after laying only a small number of eggs. These results show the potential of using biological control fungi in targeting ambrosia beetle populations either directly by killing foundresses and reducing brood production or indirectly by suppressing symbiont growth in their galleries. (C) 2016 Elsevier Inc. All rights reserved. C1 [Castrillo, Louela A.] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. [Griggs, Michael H.; Vandenberg, John D.] ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Castrillo, LA (reprint author), Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. EM lac48@cornell.edu FU Horticultural Research Institute [476, 593] FX We thank Richard Humber (USDA ARSEF Culture Collection, Ithaca, NY) for providing strain ARSEF 5198 (=F52), Laverlam (Butte, MT) for providing strain GHA and Matthew Krause (BioWorks, Inc., Victor, NY) for providing RootShield (R) WP. This work was funded in part by grants from the Horticultural Research Institute (Project nos. 476 and 593) and through an agreement between the USDA ARS and Cornell University (A. E. Hajek, cooperator). NR 42 TC 0 Z9 0 U1 8 U2 8 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD DEC PY 2016 VL 103 BP 138 EP 146 DI 10.1016/j.biocontrol.2016.09.005 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA EA2ZZ UT WOS:000386468300017 ER PT J AU Liu, HP Bauer, LS Zhao, TH Gao, RT Poland, TM AF Liu, Houping Bauer, Leah S. Zhao, Tonghai Gao, Ruitong Poland, Therese M. TI Seasonal abundance and development of the Asian longhorned beetle and natural enemy prevalence in different forest types in China SO BIOLOGICAL CONTROL LA English DT Article DE Seasonal abundance and development; Anoplophora glabripennis; Natural enemy prevalence; Dastarcus helophoroides; Forest type; China ID ANOPLOPHORA-GLABRIPENNIS COLEOPTERA; BEAUVERIA-BRONGNIARTII; DASTARCUS-HELOPHOROIDES; ENTOMOPATHOGENIC FUNGI; UNITED-STATES; FIBER BANDS; CERAMBYCIDAE; BOTHRIDERIDAE; TEMPERATURE; ARTHROPODS AB Seasonal abundance and population development of the Asian longhorned beetle (ALB), Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae), and prevalence of its natural enemies were studied on Hankow willow (Salix matsudana Koidz.) at an urban forest site (Anci) and a rural forest site (Tangerli) in Hebei province in northern China from June to October, 2002. Overall, ALB abundance was significantly lower in the urban forest in Anci than in the rural forest in Tangerli. Population density ranged from 0 to 1.0 pupae/branch, 0.1 to 0.7 adults/tree, 0.1 to 9.2 eggs/branch, and 5.9 to 18.4 larvae/branch at Anci, and 0.1 to 0.9 pupae/branch, 0.1 to 1.6 adults/tree, 0 to 22.9 eggs/branch, and 5.0 to 34.1 larvae/branch at Tangerli, respectively. A significant effect of sampling date was observed for all ALB life stages. Significantly fewer 3rd and 4th size class larvae were found in the urban forest at Anci compared to the rural forest at Tangerli. Prevalence of the gregarious larval-pupal ectoparasitoid Dastarcus helophoroides (Fairmaire) (= D. longulus) (Coleoptera: Bothrideridae) did not differ between Anci and Tangerli, but percent parasitism differed among sampling dates and peaked in June at 32.8 +/- 7.4% at Anci and 12.0 +/- 3.5% at Tangerli. Parasitism was lowest in August in Tangerli and September in Anci when most ALB larvae were at an early stage of development. The mean D. helophoroides clutch size was 5.67 +/- 0.37 parasitoids per ALB larva or pupa and did not differ between the two sites or among sample dates. Similar ALB population densities and D. helophoroides parasitism were also found at two supplemental study sites in Tianjin city (Yangcun and Xiaoxitian) where samples were only taken in June 2002. A localized epizootic of B. bassiana was observed on immature stages of ALB at Tangerli in July, resulting in an average infection rate of 2.4%. Differences in ALB population development and natural enemy prevalence between urban and rural forest site, and the potential of D. helophoroides and other natural enemies in the management of A. glabripennis are discussed. (C) 2016 Elsevier Inc. All rights reserved. C1 [Liu, Houping; Bauer, Leah S.; Poland, Therese M.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [Bauer, Leah S.; Poland, Therese M.] US Forest Serv, USDA, Northern Res Stn, Lansing, MI 48910 USA. [Zhao, Tonghai; Gao, Ruitong] Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Beijing 100091, Peoples R China. RP Liu, HP (reprint author), Penn Dept Conservat & Nat Resources, Harrisburg, PA 17105 USA. EM hliu@pa.gov FU USDA-Forest Service, North Central Research Station [00-JV-070] FX We thank Guohong Li (Chinese Academy of Forestry), Hezhi Chen and Shurong Lin (Forestry Bureau of Langfang City, Hebei) for field assistance; Jian Wu (State Forestry Administration of China), Robert. A. Haack and Deborah. L. Miller (Northern Research Station, USDA Forest Service) for their support of this study, John S. Stanovick (Northern Research Station, USDA Forest Service) for assistance with statistical analyses, and Thomas E. Luther (Northeastern Area State and Private, Forestry, Office of Knowledge Management, USDA Forest Service) for creating the map. Valuable comments by Robert A. Haack, C. Noah Koller, and three anonymous reviewers on earlier versions of the manuscript are also greatly appreciated. This research was partially funded by USDA-Forest Service, North Central Research Station Cooperative agreement 00-JV-070. NR 91 TC 0 Z9 0 U1 10 U2 10 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD DEC PY 2016 VL 103 BP 154 EP 164 DI 10.1016/j.biocontrol.2016.08.010 PG 11 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA EA2ZZ UT WOS:000386468300019 ER PT J AU Kennedy, AC AF Kennedy, Ann C. TI Pseudomonas fluorescens strains selectively suppress annual bluegrass (Poa annua L.) SO BIOLOGICAL CONTROL LA English DT Article DE Annual bluegrass; Bacteria; Biocontrol; Golf; Turf; Weed ID ARREST-FACTOR GAF; BIOLOGICAL-CONTROL; DOWNY BROME; PV. POAE; RHIZOBACTERIA; HERBICIDE; BACTERIA; JT-P482; GROWTH; CROPS AB Annual bluegrass (Poa annua L., ABG) is a cool-season annual grass that is a major weed species in turf, turfgrass-seed and sod production, and golf courses of the western United States. The life cycles of ABG and TG are very similar, but ABG is typically more competitive. Because these plants are so similar, selective control using conventional methods is limited. Pseudomonas fluorescens biovar B strain XJ3, biovar B strain XS18, and biovar A strain LRS12 are naturally-occurring soil bacteria that suppress ABG growth. These bacterial strains do not inhibit the growth of desirable turf species, other crops, or native plants. When applied to the soil and/or seeds in the fall, the bacteria establish in the soil community and increase in numbers during the cool temperatures of fall and spring, coinciding with the early root growth of ABG. These bacteria grow along roots and deliver a compound that inhibits root formation, root growth, and tiller initiation of ABG. The bacteria can also be used in combination with herbicides to inhibit the growth and seed production of standing ABG. Bacteria that selectively suppress ABG can be used to reduce the weed-seed bank in golf courses, lawns, turf, and turf-seed production. These bacteria add a formidable tool in the toolbox to fight invasive grass weeds, while limiting the need for tillage and herbicides for weed control. Published by Elsevier Inc. C1 [Kennedy, Ann C.] USDA ARS, Pullman, WA 99164 USA. RP Kennedy, AC (reprint author), Washington State Univ, 215 Johnson Hall, Pullman, WA 99164 USA. EM akennedy@wsu.edu NR 35 TC 0 Z9 0 U1 16 U2 16 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD DEC PY 2016 VL 103 BP 210 EP 217 DI 10.1016/j.biocontrol.2016.09.012 PG 8 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA EA2ZZ UT WOS:000386468300025 ER PT J AU Mohammed, A Chala, A Dejene, M Fininsa, C Hoisington, DA Sobolev, VS Arias, RS AF Mohammed, Abdi Chala, Alemayehu Dejene, Mashilla Fininsa, Chemeda Hoisington, David A. Sobolev, Victor S. Arias, Renee S. TI Aspergillus and aflatoxin in groundnut (Arachis hypogaea L.) and groundnut cake in Eastern Ethiopia SO FOOD ADDITIVES & CONTAMINANTS PART B-SURVEILLANCE LA English DT Article DE Aflatoxins; Aspergillus; groundnut; groundnut cake; Ethiopia ID AFRICA; CONTAMINATION; STRATEGIES; MARKETS; HEALTH; FUNGI; FOOD AB This study was conducted to assess major Aspergillus species and aflatoxins associated with groundnut seeds and cake in Eastern Ethiopia and evaluate growers' management practices. A total of 160 groundnut seed samples from farmers' stores and 50 groundnut cake samples from cafe and restaurants were collected. Fungal isolation was done from groundnut seed samples. Aspergillusflavus was the dominant species followed by Aspergillusparasiticus. Aflatoxin analyses of groundnut seed samples were performed using ultra performance liquid chromatography; 22.5% and 41.3% of samples were positive, with total aflatoxin concentrations of 786 and 3135ngg(-1) from 2013/2014 and 2014/2015 samples, respectively. The level of specific aflatoxin concentration varied between 0.1 and 2526ngg(-1) for B-2 and B-1, respectively. Among contaminated samples of groundnut cake, 68% exhibited aflatoxin concentration below 20ngg(-1), while as high as 158ngg(-1) aflatoxin B-1 was recorded. The study confirms high contamination of groundnut products in East Ethiopia. C1 [Mohammed, Abdi; Dejene, Mashilla; Fininsa, Chemeda] Haramaya Univ, Coll Agr & Environm Sci, POB 138, Dire Dawa, Ethiopia. [Chala, Alemayehu] Hawassa Univ, Coll Agr, Hawassa, Ethiopia. [Hoisington, David A.] Univ Georgia, Peanut & Mycotoxin Innovat Lab, Coll Agr & Environm Sci, Athens, GA 30602 USA. [Sobolev, Victor S.; Arias, Renee S.] USDA ARS, Natl Peanut Res Lab, Dawson, GA 31742 USA. RP Mohammed, A (reprint author), Haramaya Univ, Coll Agr & Environm Sci, POB 138, Dire Dawa, Ethiopia. EM abdi.mohammed22@yahoo.com FU Office of Agriculture, Research and Policy, Bureau of Food Security, U.S. Agency for International Development [AID-ECG-A-00-07-0001, 6044-21000-004-00D] FX This publication was made possible through financial support provided by the Office of Agriculture, Research and Policy, Bureau of Food Security, U.S. Agency for International Development, under the terms of Award No. AID-ECG-A-00-07-0001 to The University of Georgia as management entity for the U.S. Feed the Future Innovation Lab on Peanut Productivity and Mycotoxin Control and USDA-ARS-National Peanut Research Laboratory: CRIS project 6044-21000-004-00D. The opinions expressed herein are those of the author(s) and do not necessarily reflect the views of the U.S. Agency for International Development. NR 44 TC 0 Z9 0 U1 1 U2 1 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND SN 1939-3210 EI 1939-3229 J9 FOOD ADDIT CONTAM B JI Food Addit. Contam. Part B-Surveill. PD DEC PY 2016 VL 9 IS 4 BP 290 EP 298 DI 10.1080/19393210.2016.1216468 PG 9 WC Chemistry, Applied; Food Science & Technology; Toxicology SC Chemistry; Food Science & Technology; Toxicology GA EA4YY UT WOS:000386624300009 PM 27748169 ER PT J AU Xu, JY Inglett, GE Liu, SX Boddu, VM AF Xu, Jingyuan Inglett, George E. Liu, Sean X. Boddu, Veera M. TI Micro-Heterogeneity and Micro-Rheological Properties of High-Viscosity Barley beta-Glucan Solutions Studied by Diffusing Wave Spectroscopy (DWS) SO FOOD BIOPHYSICS LA English DT Article DE beta-glucan; Micro-heterogeneity; Micro-rheology; Diffusingwave spectroscopy (DWS) ID ACTIN-FILAMENT NETWORKS; CONCENTRATED ISOTROPIC SOLUTIONS; SERUM LDL-CHOLESTEROL; HULL-LESS BARLEY; SEMIFLEXIBLE POLYMERS; PARTICLE-TRACKING; OAT BRAN; PHYSICOCHEMICAL PROPERTIES; VISCOELASTIC MODULI; COMPLEX FLUIDS AB Soluble fiber beta-glucan is one of the key dietary materials in healthy food products known for reducing serum cholesterol levels. The micro-structural heterogeneity and micro-rheology of high-viscosity barley beta-glucan solutions were investigated by the diffusing wave spectroscopy (DWS) technology. By comparing the mean-square displacement (MSD) of the microspheres imbedded in eight concentrations of beta-glucan solutions, we found that the solutions exhibited nearly homogeneous behavior at aecurrency sign0.1 %, but the material showed a clear degree of heterogeneity at aeyen0.25 %. Micro-rheology investigation revealed that beta-glucan solutions displayed nearly perfect viscous behavior at aecurrency sign0.1 %, but the property changed into viscoelastic one at aeyen0.25 %. The magnitude of high-frequency viscoelastic moduli for the 0.25 % - 0.75 % beta-glucan solutions can be characterized by EuroG*Euro ae omega(3/4), which is the semi-flexible polymer behavior. However, the magnitude of high-frequency viscoelastic moduli (EuroG*Euro) for the 1.0 % - 1.25 % beta-glucan solutions is proportional to omega(1/2), which is the flexible polymer behavior. All micro-structural heterogeneity and micro-rheological property shifts occurred in relatively small concentration ranges. C1 [Xu, Jingyuan; Inglett, George E.; Liu, Sean X.; Boddu, Veera M.] ARS, Natl Ctr Agr Utilizat Res, USDA, 1815 North Univ St, Peoria, IL 61604 USA. RP Xu, JY (reprint author), ARS, Natl Ctr Agr Utilizat Res, USDA, 1815 North Univ St, Peoria, IL 61604 USA. EM james.xu@ars.usda.gov FU US Department of Agriculture, Agricultural Research Service FX The technical assistance of Elizabeth Krietemeyer is gratefully acknowledged. This work was financially supported by US Department of Agriculture, Agricultural Research Service. NR 36 TC 0 Z9 0 U1 6 U2 6 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1557-1858 EI 1557-1866 J9 FOOD BIOPHYS JI Food Biophys. PD DEC PY 2016 VL 11 IS 4 BP 339 EP 344 DI 10.1007/s11483-016-9447-2 PG 6 WC Food Science & Technology SC Food Science & Technology GA EA3PC UT WOS:000386514800004 ER PT J AU Jang, JR Hong, EM Song, I Kang, MS Cho, JY Cho, YK AF Jang, Jeong-Ryeol Hong, Eun-Mi Song, Inhong Kang, Moon-Seong Cho, Jae-Young Cho, Young-Kweon TI Impact of Different Fertilizer Types on Nutrient Pollutant Loads from Rice Paddy Fields in South Korea SO IRRIGATION AND DRAINAGE LA English DT Article; Proceedings Paper CT 22nd International Congress of the International-Commission-on-Irrigation-and-Drainage (ICID) on Securing Water for Food and Rural Community under Climate Change CY SEP, 2014 CL Gwangju, SOUTH KOREA SP Int Commiss Irrigat & Drainage DE fertilizer; liquid manure; NPS nutrient loads; rice paddy; slow-release fertilizer; swine compost ID LAND AB The objective of this study was to evaluate the effects of best management practices (BMPs) on nutrient load reduction from rice paddy fields. Three different fertilizations were considered as the study BMPs: slow-release chemical fertilizer (SC), swine compost manure (CM) and swine liquid manure (LM). Two experiments were conducted independently in fields with sizes of 100x40m in the Saemangeum region. One experiment was conducted at Iksan City to compare conventional chemical fertilizer (CA) with SC in non-point source (NPS) surface runoff, while the other was conducted at Kimje City to compare chemical fertilizer (CB) with CM and LM. Nutrient surface discharge from the SC-treated paddy was 50-55% less than from the CA-treated paddy. The nutrient loads from the LM- and CM-treated fields were 18-48% less than the CB field. This indicates that the SC, CM and LM applications can effectively reduce paddy surface nutrient loads and thus be alternatives to chemical fertilizer application. However, rice yield was slightly decreased (by approximately 7.5%) in the SC-treated field compared to the CA-treated field. With certain compensation measures for the economic losses, the alternative fertilization can be sustainable ways for paddy nutrient load reduction. Copyright (c) 2016 John Wiley & Sons, Ltd. C1 [Jang, Jeong-Ryeol; Cho, Young-Kweon] Korea Rural Community Corp, Rural Res Inst, Gyeonggi, South Korea. [Hong, Eun-Mi] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD USA. [Song, Inhong] Seoul Natl Univ, Dept Rural Syst Engn, Seoul, South Korea. [Song, Inhong] Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul, South Korea. [Kang, Moon-Seong] Seoul Natl Univ, Dept Rural Syst Engn, Inst Green Bio Sci & Technol, Seoul, South Korea. [Kang, Moon-Seong] Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul, South Korea. [Cho, Jae-Young] Jeonbuk Natl Univ, Dept Bioenvironm Chem, Jeonju, South Korea. RP Kang, MS (reprint author), Seoul Natl Univ, Dept Rural Syst Engn, Inst Green Bio Sci & Technol, Seoul, South Korea.; Kang, MS (reprint author), Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul, South Korea. EM mskang@snu.ac.kr NR 23 TC 0 Z9 0 U1 5 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1531-0353 EI 1531-0361 J9 IRRIG DRAIN JI Irrig. Drain. PD DEC PY 2016 VL 65 SU 2 SI SI BP 105 EP 111 DI 10.1002/ird.2041 PG 7 WC Agronomy; Water Resources SC Agriculture; Water Resources GA EA9GE UT WOS:000386949600012 ER PT J AU McNeill, CA Allan, SA Koehler, PG Pereira, RM Weeks, ENI AF McNeill, C. A. Allan, S. A. Koehler, P. G. Pereira, R. M. Weeks, E. N. I. TI Vision in the common bed bug Cimex lectularius L. (Hemiptera: Cimicidae): eye morphology and spectral sensitivity SO MEDICAL AND VETERINARY ENTOMOLOGY LA English DT Article DE Electrophysiology; insect vision; light intensity; ommatidia; wavelength ID TABANUS-NIGROVITTATUS DIPTERA; COLOR-VISION; TRIATOMA-INFESTANS; VISUAL ECOLOGY; BEHAVIORAL-RESPONSES; COMPOUND EYE; LIGHT; INSECTS; EVOLUTION; FLIES AB Bed bugs as pests of public health importance recently experienced a resurgence in populations throughout the U.S. and other countries. Consequently, recent research efforts have focused on improving understanding of bed bug physiology and behaviour to improve management. While few studies have investigated the visual capabilities of bed bugs, the present study focused specifically on eye morphology and spectral sensitivity. A 3-D imaging technique was used to document bed bug eye morphology from the first instar through adult and revealed morphological characteristics that differentiate the common bed bug from the tropical bed bug as well as sex-specific differences. Electrophysiological measurements were used to evaluate the spectral sensitivity of adult bed bugs. Male bed bugs were more responsive than females at some wavelengths. Electrophysiological studies provided evidence for at least one photoreceptor with a spectral sensitivity curve peak in the green ((max) 520nm) region of the spectrum. The broadened long wavelength portion of the spectral sensitivity curve may potentially indicate another photoreceptor in the yellow-green ((max) 550nm) portion of the spectrum or screening pigments. Understanding more about bed bug visual biology is vital for designing traps, which are an important component of integrated bed bug management. C1 [McNeill, C. A.] Union Coll, Dept Sci & Math, 3800 S 48th St, Lincoln, NE 68506 USA. [Allan, S. A.] USDA ARS, Ctr Med Agr & Vet Entomol, Insect Behav & Biocontrol Res, Gainesville, FL USA. [Koehler, P. G.; Pereira, R. M.; Weeks, E. N. I.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. RP McNeill, CA (reprint author), Union Coll, Dept Sci & Math, 3800 S 48th St, Lincoln, NE 68506 USA. EM comcneil@ucollege.edu OI Pereira, Roberto/0000-0002-5618-7690 FU Florida Pest Management Association; University of Florida Endowed Professor Fund FX We wish to thank the Urban Entomology Laboratory at the University of Florida for supplying the cultured strain of the bed bugs used in these experiments. Special thanks to Seth McNeill (Engineering and Computer Science, Union College), Salvador Gezan (School of Forest Resources and Conservation, University of Florida) and Aria Bodden (Division of Business, Union College) for helpful comments and insights during the preparation of the manuscript. We are also grateful to Marc Branham for suggestions on documenting eye morphology, and Lyle Buss for help with the Automontage software (Entomology and Nematology Department, University of Florida, FL, U.S.A). Funding for this research was provided by the Florida Pest Management Association and University of Florida Endowed Professor Fund. NR 60 TC 0 Z9 0 U1 19 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0269-283X EI 1365-2915 J9 MED VET ENTOMOL JI Med. Vet. Entomol. PD DEC PY 2016 VL 30 IS 4 BP 426 EP 434 DI 10.1111/mve.12195 PG 9 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA EB0HF UT WOS:000387024000008 PM 27670265 ER PT J AU Wright, AA Molin, WT Nandula, VK AF Wright, Alice A. Molin, William T. Nandula, Vijay K. TI Distinguishing between weedy Amaranthus species based on intron 1 sequences from the 5-enolpyruvylshikimate-3-phosphate synthase gene SO PEST MANAGEMENT SCIENCE LA English DT Article; Proceedings Paper CT Annual Meeting of the Weed-Science-Society-of-America CY 2015 CL Lexington, KY SP Weed Sci Soc Amer DE PCR; hybrids ID HERBICIDE RESISTANCE; PHYLOGENETIC ANALYSIS; NUCLEAR GENES; HYBRIDIZATION; WILD; UTILITY; FLOW AB BACKGROUNDHybridization between Amaranthus species and the potential for herbicide resistance to be transferred by hybridization are of growing concern in the weed science community. Early detection of evolved herbicide resistance and hybrids expressing resistance to single or multiple herbicides is important to develop an effective control strategy. RESULTSA PCR test was developed for quick identification of weedy amaranths and any hybrids. The sequences of intron 1 for the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS; EC 2.5.1.19) gene were determined for Amaranthus palmeri, A. spinosus, A. retroflexus, A. blitoides, A. viridis, A. tuberculatus and A. hybridus. These sequences were aligned and primers were developed in areas where the sequence differed between species. Species-specific primers and cycle conditions were successfully developed. These primers produce a single robust band only for the species for which they were designed. CONCLUSIONThe PCR techniques described here allow identification of a weedy amaranth or suspect hybrid in a few hours. Using a similar target, it may be possible to design simple PCR tests to identify even more difficult to distinguish weed species or weeds prone to interspecific hybridization. Published 2016. This article is a U.S. Government work and is in the public domain in the USA. C1 [Wright, Alice A.; Molin, William T.; Nandula, Vijay K.] USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. RP Molin, WT (reprint author), USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. EM william.molin@ars.usda.gov NR 21 TC 0 Z9 0 U1 1 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD DEC PY 2016 VL 72 IS 12 BP 2347 EP 2354 DI 10.1002/ps.4280 PG 8 WC Agronomy; Entomology SC Agriculture; Entomology GA EA8ZB UT WOS:000386927800018 PM 27005944 ER PT J AU Yang, M Hall, J Fan, ZQ Regouski, M Meng, QG Rutigliano, HM Stott, R Rood, KA Panter, KE Polejaeva, IA AF Yang, Min Hall, Justin Fan, Zhiqiang Regouski, Misha Meng, Qinggang Rutigliano, Heloisa M. Stott, Rusty Rood, Kerry A. Panter, Kip E. Polejaeva, Irina A. TI Oocytes from small and large follicles exhibit similar development competence following goat cloning despite their differences in meiotic and cytoplasmic maturation SO THERIOGENOLOGY LA English DT Article DE Goat somatic cell nuclear transfer; Brilliant cresyl blue; Real-time PCR; Oocyte activation; Oocyte gene expression ID BRILLIANT CRESYL BLUE; IN-VITRO MATURATION; OVARIAN FOLLICULAR DEVELOPMENT; ADULT SOMATIC-CELLS; EMBRYO DEVELOPMENT; NUCLEAR TRANSFER; ENDOTHELIAL-CELLS; TYROSINE KINASES; IMPRINTED GENES; CALCIUM INFLUX AB Reduced developmental competence after IVF has been reported using oocyte derived from small follicles in several species including cattle, sheep, and goats. No information is currently available about the effect of follicle size of the cytoplast donor on in vivo development after somatic cell nuclear transfer (SCNT) in goats. Oocytes collected from large (>= 3 mm) and small follicles (<3 mm) were examined for maturation and in vivo developmental competence after SCNT. Significantly greater maturation rate was observed in oocytes derived from large follicles compared with that of small follicles (51.6% and 33.7%, P < 0.05). Greater percent of large follicle oocytes exhibited a low glucose 6-phosphate dehydrogenase activity at germinal vesicle stage compared with small follicle oocytes (54.9% and 38.7%, P < 0.05). Relative mRNA expression analysis of 48 genes associated with embryonic and fetal development revealed that three genes (MATER, IGF2R, and GRB10) had higher level of expression in metaphase II oocytes from large follicles compared with oocytes from small follicles. Nevertheless, no difference was observed in pregnancy rates (333% vs. 47.1%) and birth rates (22.2% vs. 16.7%) after SCNT between the large and small follicle groups). These results indicate that metaphase II cytoplasts from small and large follicles have similar developmental competence when used in goat SCNT. (C) 2016 Elsevier Inc. All rights reserved. C1 [Yang, Min; Hall, Justin; Fan, Zhiqiang; Regouski, Misha; Meng, Qinggang; Rutigliano, Heloisa M.; Stott, Rusty; Rood, Kerry A.; Polejaeva, Irina A.] Utah State Univ, Dept Anim Dairy & Vet Sci, Logan, UT 84322 USA. [Rutigliano, Heloisa M.; Stott, Rusty; Rood, Kerry A.] Utah State Univ, Sch Vet Med, Logan, UT 84322 USA. [Panter, Kip E.] USDA ARS, Poisonous Plant Res Lab, 1150 E 1400 N, Logan, UT 84321 USA. RP Polejaeva, IA (reprint author), Utah State Univ, Dept Anim Dairy & Vet Sci, Logan, UT 84322 USA. EM irina.polejaeva@usu.edu FU Utah Science Technology and Research Initiative, Utah Multidisciplinary Arrhythmia Consortium; Utah Agricultural Experimental Stations; American Heart Association [13GRNT16850082] FX The authors would like to thank Dave Forester and Kirsten Gash for assisting with embryo transfer procedures and animal handling. Additionally, IAP acknowledges support from the Utah Science Technology and Research Initiative, Utah Multidisciplinary Arrhythmia Consortium, the Utah Agricultural Experimental Stations, and the American Heart Association grant 13GRNT16850082. NR 64 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0093-691X EI 1879-3231 J9 THERIOGENOLOGY JI Theriogenology PD DEC PY 2016 VL 86 IS 9 BP 2302 EP 2311 DI 10.1016/j.theriogenology.2016.07.026 PG 10 WC Reproductive Biology; Veterinary Sciences SC Reproductive Biology; Veterinary Sciences GA EA3XC UT WOS:000386541600025 PM 27650944 ER PT J AU Dimitrov, KM Bolotin, V Muzyka, D Goraichuk, IV Solodiankin, O Gerilovych, A Stegniy, B Goujgoulova, GV Silko, NY Pantin-Jackwood, MJ Miller, PJ Afonso, CL AF Dimitrov, Kiril M. Bolotin, Vitaliy Muzyka, Denys Goraichuk, Iryna V. Solodiankin, Olexii Gerilovych, Anton Stegniy, Borys Goujgoulova, Gabriela V. Silko, Nikita Y. Pantin-Jackwood, Mary J. Miller, Patti J. Afonso, Claudio L. TI Repeated isolation of virulent Newcastle disease viruses of sub-genotype VIId from backyard chickens in Bulgaria and Ukraine between 2002 and 2013 SO ARCHIVES OF VIROLOGY LA English DT Article ID OUTBREAKS; CHINA; AFRICA; SEQUENCE; STRAINS; EUROPE; INFECTION; POULTRY; REGION; GEESE AB Here, we report the circulation of highly related virulent Newcastle disease viruses (NDV) in Bulgaria and Ukraine from 2002 until 2013. All of these NDV isolates have the same virulence-associated cleavage site ("(113)RQKRa dagger"F-117"), and selected ones have intracerebral pathogenicity index values ranging from 1.61 to 1.96. These isolates are most closely related to viruses circulating in Eastern Europe, followed by viruses isolated in Asia during the same period of time. Interestingly, the majority of the viruses were isolated from backyard poultry, suggesting the possibility of a "domestic" or "urban" cycle of maintenance. The molecular characterization of the nucleotide sequence of the complete fusion protein gene of the studied viruses suggests continued circulation of virulent NDV of sub-genotype VIId in Eastern Europe, with occasional introductions from Asia. Furthermore, the high level of genetic similarity among those isolates suggests that the NDV isolates of sub-genotype VIId from Bulgaria and Ukraine may have been part of a broader epizootic process in Eastern Europe rather than separate introductions from Asia or Africa. The continuous monitoring of backyard poultry flocks for the presence of circulating virulent NDV strains will allow early identification of Newcastle disease outbreaks. C1 [Dimitrov, Kiril M.; Goraichuk, Iryna V.; Pantin-Jackwood, Mary J.; Miller, Patti J.; Afonso, Claudio L.] ARS, Exot & Emerging Avian Viral Dis Res Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr,USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. [Dimitrov, Kiril M.; Goujgoulova, Gabriela V.] Natl Diagnost & Res Vet Med Inst, 15 Pencho Slaveikov Blvd, Sofia 1606, Bulgaria. [Bolotin, Vitaliy; Muzyka, Denys; Goraichuk, Iryna V.; Solodiankin, Olexii; Gerilovych, Anton; Stegniy, Borys] Natl Sci Ctr, Inst Expt & Clin Vet Med, 83 Pushkinskaya St, UA-61023 Kharkov, Ukraine. [Silko, Nikita Y.] State Res Ctr Virol & Biotechnol VECTOR, Koltsov 630559, Novosibirsk, Russia. RP Afonso, CL (reprint author), ARS, Exot & Emerging Avian Viral Dis Res Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr,USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. EM Claudio.Afonso@ars.usda.gov FU Defense Threat Reduction Agency, BAA project [FRCALL12-6-3412-0005]; USDA, ARS CRIS Project [6040-32000-064] FX This work was supported by the Defense Threat Reduction Agency, BAA project FRCALL12-6-3412-0005, and by the USDA, ARS CRIS Project 6040-32000-064. The authors declare that they have no conflict of interest. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. This manuscript was improved by comments from an editor and an anonymous reviewer. NR 50 TC 0 Z9 0 U1 5 U2 5 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD DEC PY 2016 VL 161 IS 12 BP 3345 EP 3353 DI 10.1007/s00705-016-3033-2 PG 9 WC Virology SC Virology GA DZ7TB UT WOS:000386068400005 PM 27581808 ER PT J AU Harper, SJ Killiny, N Tatineni, S Gowda, S Cowell, SJ Shilts, T Dawson, WO AF Harper, S. J. Killiny, N. Tatineni, S. Gowda, S. Cowell, S. J. Shilts, T. Dawson, W. O. TI Sequence variation in two genes determines the efficacy of transmission of citrus tristeza virus by the brown citrus aphid SO ARCHIVES OF VIROLOGY LA English DT Article ID INFECTIOUS-YELLOWS-VIRUS; MINOR COAT PROTEIN; PLANT-VIRUSES; INSECT VECTOR; MOSAIC-VIRUS; POTYVIRUS; VIRION AB Vector transmission is an important part of the viral infection cycle, yet for many viruses little is known about this process, or how viral sequence variation affects transmission efficacy. Here we examined the effect of substituting genes from the highly transmissible FS577 isolate of citrus tristeza virus (CTV) in to the poorly transmissible T36-based infectious clone. We found that introducing p65 or p61 sequences from FS577 significantly increased transmission efficacy. Interestingly, replacement of both genes produced a greater increase than either gene alone, suggesting that CTV transmission requires the concerted action of co-evolved p65 and p61 proteins. C1 [Harper, S. J.; Killiny, N.; Gowda, S.; Cowell, S. J.; Shilts, T.; Dawson, W. O.] Univ Florida, Dept Plant Pathol, 700 Expt Stn Rd, Lake Alfred, FL 33850 USA. [Tatineni, S.] Univ Nebraska, ARS, USDA, Lincoln, NE USA. [Tatineni, S.] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. RP Harper, SJ (reprint author), Univ Florida, Dept Plant Pathol, 700 Expt Stn Rd, Lake Alfred, FL 33850 USA. EM sjharper@ufl.edu FU Southern Gardens Citrus; J. R. and Addie S. Graves family; UF Agricultural Experiment Station FX The authors wish to acknowledge the assistance of C. Robertson and R. McCoy for virus propagation, E. Carter for sampling of plants, and C.T. Bierman for performing ELISA assays. This research was supported by grants from Southern Gardens Citrus, by an endowment from the J. R. and Addie S. Graves family, and the UF Agricultural Experiment Station. NR 22 TC 0 Z9 0 U1 27 U2 27 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD DEC PY 2016 VL 161 IS 12 BP 3555 EP 3559 DI 10.1007/s00705-016-3070-x PG 5 WC Virology SC Virology GA DZ7TB UT WOS:000386068400028 PM 27644950 ER PT J AU Selin-Rani, S Senthil-Nathan, S Thanigaivel, A Vasantha-Srinivasan, P Edwin, ES Ponsankar, A Lija-Escaline, J Kalaivani, K Abdel-Megeed, A Hunter, WB Alessandro, RT AF Selin-Rani, Selvaraj Senthil-Nathan, Sengottayan Thanigaivel, Annamalai Vasantha-Srinivasan, Prabhakaran Edwin, Edward-Sam Ponsankar, Athirstam Lija-Escaline, Jalasteen Kalaivani, Kandaswamy Abdel-Megeed, Ahmed Hunter, Wayne B. Alessandro, Rocco T. TI Toxicity and physiological effect of quercetin on generalist herbivore, Spodoptera litura Fab. and a non-target earthworm Eisenia fetida Savigny SO CHEMOSPHERE LA English DT Article DE Asthma-weed; Biorational insecticide; Secondary metabolites; Cut worm; Nutritional indices; Mortality; Earthworm toxicity; Histology ID LISTON DIPTERA-CULICIDAE; INSECTICIDES; PESTICIDES; CONSTITUENTS; CYPERMETHRIN; INHIBITION; FLAVONOIDS; EXTRACT; INDICA AB A novel flavonoid, quercetin, was isolated from the medicinal plant Euphorbia hirta L through chromatography techniques including: TLC, Column chromatography, NMR and then screened for toxicity to larvae of Spodoptera litura Fab. Bioassays were used to analyze pupal weight, survival rate, fecundity, egg hatchability, population growth index, Nutritional index and histopathology of treated larvae at a range of E. hirta extract concentrations. Results of toxicity assays demonstrated that, 6 ppm of quercetin caused 94.6% mortality of second, 91.8% of third, 88% of fourth, and 85.2% of fifth instars respectively. The lethal concentrations (LC50 and LC90) was calculated as 10.88 and 69.91 ppm for fourth instar larvae. The changes in consumption ratio and approximate digestibility produced a reduction in growth rates. Histopathology examinations revealed that the cell organelles were severely infected. Analyses of earthworm toxicity effects resulted in significantly lower rates compared to synthetic insecticides (chloropyrifos and cypermethrin). These results suggests that the botanical compound (quercetin), could have a part as a new biorational product which provides an ecofriendly alternative. Validation of the potential of quercetin, still needs to be demonstrated under field conditions, where formulation will be important in maintaining the activity. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Selin-Rani, Selvaraj; Senthil-Nathan, Sengottayan; Thanigaivel, Annamalai; Vasantha-Srinivasan, Prabhakaran; Edwin, Edward-Sam; Ponsankar, Athirstam; Lija-Escaline, Jalasteen] Manonmaniam Sundaranar Univ, Div Biopesticides & Environm Toxicol, Sri Paramakalyani Ctr Excellence Environm Sci, Tirunelveli 627412, Tamil Nadu, India. [Kalaivani, Kandaswamy] Sri Parasakthi Coll Women, Dept Zool, Post Grad & Res Ctr, Tirunelveli 627802, Tamil Nadu, India. [Abdel-Megeed, Ahmed] Univ Alexandria, Fac Agr, Dept Plant Protect, POB 21531, Alexandria 21526, Egypt. [Hunter, Wayne B.] USDA, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. [Alessandro, Rocco T.] Treasure Coast Chem Consultants LLC, 107 Lakes End Dr, Apt B Ft Pierce, FL 34982 USA. RP Senthil-Nathan, S (reprint author), Manonmaniam Sundaranar Univ, Div Biopesticides & Environm Toxicol, Sri Paramakalyani Ctr Excellence Environm Sci, Tirunelveli 627412, Tamil Nadu, India. EM senthil@msuniv.ac.in OI Senthil Nathan, Sengottayan/0000-0003-0839-2021; Abdel-Megeed, Ahmed/0000-0002-9777-4808 FU Department of Science and Technology (DST), Government of India (DST/INSPIRE) [2013/456] FX Department of Science and Technology (DST), Government of India (DST/INSPIRE Fellowship/2013/456). NR 73 TC 1 Z9 1 U1 12 U2 12 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0045-6535 EI 1879-1298 J9 CHEMOSPHERE JI Chemosphere PD DEC PY 2016 VL 165 BP 257 EP 267 DI 10.1016/j.chemosphere.2016.08.136 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA EA2DE UT WOS:000386402200030 PM 27657818 ER PT J AU Ariani, A Romeo, S Groover, AT Sebastiani, L AF Ariani, Andrea Romeo, Stefania Groover, Andrew T. Sebastiani, Luca TI Comparative epigenomic and transcriptomic analysis of Populus roots under excess Zn SO ENVIRONMENTAL AND EXPERIMENTAL BOTANY LA English DT Article DE Phytoremediation; Zn toxicity; ChIP-Seq; RNA-Seq; Metal homeostasis; Populus x canadensis I-214 clone ID EURAMERICANA CLONE I-214; PENTOSE-PHOSPHATE PATHWAY; EPIGENETIC REGULATION; ARABIDOPSIS-THALIANA; GLUTAMINE-SYNTHETASE; METAL HOMEOSTASIS; STRESS RESPONSES; IRON-DEFICIENCY; CHIP-SEQ; PLANTS AB Epigenetic modifications of chromatin structure are extremely important in mediating stress responses in plants. Epigenetic modifications are especially important in perennial species such as trees, where they contribute to phenotypic plasticity and adaptation to unfavourable environments. Populus is a model for physiological studies of trees, and is suitable for phytoremediation of Zn-contaminated soils. Currently, epigenetic modifications involved in Zn stress response are poorly characterized for Populus. Here, we compared changes in epigenetic modifications under excess Zn using chromatin immunoprecipitation sequencing (Chip-Seq) for two histone modifications associated with highly expressed genes (H3K4me3) and repressed genes (H3K27me3) in roots of Populus x canadensis I-214. Chip-Seq data were integrated with RNA-Seq transcript abundance data to examine how epigenetic modifications affect gene expression. These analyses showed that genes with a H3K4me3 modification are generally high expressed, while genes with a H3K27me3 modification on the 5'-UTR are mainly low-expressed. H3K4me3 modifications in roots under excess Zn condition were enriched in genes involved in carbon (C) catabolism, nitrogen (N) metabolism, and in regulation of sub-cellular vesicular trafficking. These results are consistent with Zn redistribution at a sub-cellular level to buffer Zn-induced nutrient imbalance and osmotic stress in Zn-stressed roots. In contrast H3K27me3 modifications were enriched primarily in genes involved in photosynthetic processes. Together our results provide a useful resource for understanding epigenetic modifications in response to excess Zn in Populus roots, and constitute a starting point for the identification of epigenetic markers and improving phytoremediation potential in this species. (C) 2016 Elsevier B.V. All rights reserved. C1 [Ariani, Andrea; Romeo, Stefania; Sebastiani, Luca] Scuola Super Sant Anna, Inst Life Sci, BioLabs, Piazza Martiri Liberta 33, I-56127 Pisa, Italy. [Groover, Andrew T.] US Forest Serv, Pacific Southwest Res Stn, Davis, CA 95618 USA. [Groover, Andrew T.] Univ Calif Davis, Dept Plant Biol, Davis, CA 95618 USA. RP Ariani, A (reprint author), Univ Calif Davis, Dept Plant Sci MS1, 1 Shields Ave, Davis, CA 95616 USA. EM aariani@ucdavis.edu FU NIH [S10RR029668, S10RR027303] FX This work used the Vincent J. Coates Genomics Sequencing Laboratory at UC Berkeley, supported by NIH S10 Instrumentation Grants S10RR029668 and S10RR027303. NR 80 TC 0 Z9 0 U1 23 U2 23 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0098-8472 EI 1873-7307 J9 ENVIRON EXP BOT JI Environ. Exp. Bot. PD DEC PY 2016 VL 132 BP 16 EP 27 DI 10.1016/j.envexpbot.2016.08.005 PG 12 WC Plant Sciences; Environmental Sciences SC Plant Sciences; Environmental Sciences & Ecology GA EA2HZ UT WOS:000386414700002 ER PT J AU Zhao, X Christianson, LE Harmel, D Pittelkow, CM AF Zhao, Xu Christianson, Laura E. Harmel, Daren Pittelkow, Cameron M. TI Assessment of drainage nitrogen losses on a yield-scaled basis SO FIELD CROPS RESEARCH LA English DT Article DE Agricultural drainage N losses; Yield; N surplus; Optimal N rate; Precipitation ID MISSISSIPPI RIVER-BASIN; GULF-OF-MEXICO; TILE DRAINAGE; WATER-QUALITY; SUBSURFACE DRAINAGE; CROPPING SYSTEMS; USE EFFICIENCY; NUTRIENT LOAD; N2O EMISSIONS; NITRATE LOSS AB Subsurface nitrogen (N) losses represent a major environmental concern in agriculture, particularly from fields containing artificial drainage to prevent saturated soil conditions and increase crop production. To develop sustainable intensification strategies and achieve high yields with minimal environmental impacts, N losses are increasingly evaluated with respect to crop productivity on a "yield-scaled" basis, yet little information is available to address the current challenge of balancing crop yields and drainage N losses from intensive maize production systems in the U.S. Midwest by using this metric. In the present study, a meta-analysis was conducted using 31 studies with 381 observations from a publicly available nutrient loss drainage database (Measured Annual Nutrient loads from Agricultural Environments, MANAGE) to address this issue. Results showed that increasing N rates enhanced yields but had weak effects on area- and yield-scaled drainage N losses. In contrast, yield-scaled drainage N losses responded exponentially to N surplus (estimated as N application rate minus above-ground crop N uptake). Relative precipitation during the drainage monitoring period strongly influenced area- and yield-scaled drainage N losses. Maize-soybean rotations and silt loam soils had lower yield-scaled drainage N losses compared to continuous maize and clay loam soils, respectively, whereas tillage practices had little impact on yield scaled drainage N losses. To meet the growing challenge of achieving high yields with minimal impacts on water quality, these results suggest that evaluating drainage N losses on a yield-scaled basis may complement the more conventional approach of evaluating N losses on an areas basis for maize systems in this region. (C) 2016 Elsevier B.V. All rights reserved. C1 [Zhao, Xu; Christianson, Laura E.; Pittelkow, Cameron M.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Zhao, Xu] Chinese Acad Sci, State Key Lab Soil & Sustainable Agr, Changshu Natl Agroecosyst Observat & Res Stn, 71 East Beijing Rd, Nanjing 210008, Jiangsu, Peoples R China. [Harmel, Daren] USDA ARS, Grassland Soil & Water Res Lab, 808 East Blackland Rd, Temple, TX 76502 USA. RP Zhao, X; Pittelkow, CM (reprint author), Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. EM zhaoxu@issas.ac.cn; cmpitt@illinois.edu FU 4R Research Fund; Youth Innovation Promotion Association; Chinese Academy of Sciences [2015249]; Illinois Nutrient Research & Education Council FX The authors gratefully acknowledge the 4R Research Fund for supporting development of the MANAGE Drain Load database, the support of the Illinois Nutrient Research & Education Council, and the Youth Innovation Promotion Association, Chinese Academy of Sciences (Member No. 2015249). NR 58 TC 1 Z9 1 U1 28 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4290 EI 1872-6852 J9 FIELD CROP RES JI Field Crop. Res. PD DEC PY 2016 VL 199 BP 156 EP 166 DI 10.1016/j.fcr.2016.07.015 PG 11 WC Agronomy SC Agriculture GA DZ9IZ UT WOS:000386189900016 ER PT J AU Wang, SG Zhang, H Lin, SY Hse, CY Ding, YL AF Wang, Shuguang Zhang, Hui Lin, Shuyan Hse, Chungyun Ding, Yulong TI Anatomical characteristics of fusoid cells and vascular bundles in Fargesia yunnanensis leaves SO JOURNAL OF FORESTRY RESEARCH LA English DT Article DE Fusoid cell; Sheath cell; Chloroplast; Transport ID PARAVENOUS MESOPHYLL; SHEATH-CELLS; ASSIMILATE TRANSFER; SOYBEAN LEAVES; MESTOME SHEATH; WHEAT LEAVES; LEAF; COMPARTMENTATION; ULTRASTRUCTURE; ENVIRONMENT AB As of today, the functions of fusoid cell, and the transport and loading pathways of photoassimilate in bamboo leaves are still not clear. In this paper, the leaves of Fargesia yunnanensis from a greenhouse and the wild were respectively used as samples to analyze the anatomical characteristics of fusoid cells and vascular bundles. The results showed that the bamboo leaves from greenhouse got shorter and thinner with fewer layers of palisade parenchyma cells than those from the wild. The volumes of fusoid cells were also increased. Fusoid cells originated from a huge parenchyma cell as testified by the observed nuclei. Several fusoid cells usually formed one cell complex close to the midrib. Crystals were detected in fusoid cells but no pits or plasmodesmata on their walls, suggesting that fusoid cells had the function of regulating water. The presence of fusoid cells determined the major difference between a leaf blade and sheath. There were prominent chloroplasts with simple stroma lamellae in the parenchymatous bundle sheath cells and starch grains were also observed in these chloroplast. Photoassimilates could be transported across vascular bundle sheath via symplasmic pathways for an abundant of plasmodesmata in sheath cell walls, and transported into phloem tube by apoplastic pathway as there were no pits in the walls of companion cells and phloem tubes. C1 [Wang, Shuguang; Zhang, Hui] Southwest Forestry Univ, Fac Life Sci, Kunming 650224, Yunnan, Peoples R China. [Lin, Shuyan; Ding, Yulong] Nanjing Forestry Univ, Bamboo Res Inst, Nanjing 210037, Jiangsu, Peoples R China. [Hse, Chungyun] US Forest Serv, Southern Res Stn, USDA, Pinville, LA 71360 USA. RP Ding, YL (reprint author), Nanjing Forestry Univ, Bamboo Res Inst, Nanjing 210037, Jiangsu, Peoples R China. EM ylding@vip.163.com FU national "Twelfth Five-Year'' scientific and technological support plan subject [2012BAD23B05]; National Natural Science Fund of China [31560196] FX The project was fully funded by the national "Twelfth Five-Year'' scientific and technological support plan subject (2012BAD23B05) and the National Natural Science Fund of China (31560196). NR 35 TC 0 Z9 0 U1 5 U2 5 PU NORTHEAST FORESTRY UNIV PI HARBIN PA NO 26 HEXING RD, XIANGFANG DISTRICT, HARBIN, 150040, PEOPLES R CHINA SN 1007-662X EI 1993-0607 J9 J FORESTRY RES JI J. For. Res. PD DEC PY 2016 VL 27 IS 6 BP 1237 EP 1247 DI 10.1007/s11676-016-0271-9 PG 11 WC Forestry SC Forestry GA EA1NB UT WOS:000386357900004 ER PT J AU Sun, DY Zhang, XG Li, SH Jiang, CZ Zhang, YL Niu, LX AF Sun, Daoyang Zhang, Xinguo Li, Shaohua Jiang, Cai-Zhong Zhang, Yanlong Niu, Lixin TI LrABCF1, a GCN-type ATP-binding cassette transporter from Lilium regale, is involved in defense responses against viral and fungal pathogens SO PLANTA LA English DT Article DE Wild Lilium; ABC transporter; Cucumber mosaic virus; Lily mottle virus; Tobacco rattle virus; Botrytis cinerea ID EIF2-ALPHA KINASE GCN2; ARABIDOPSIS-THALIANA; ABC TRANSPORTER; GENE-EXPRESSION; TRANSLATIONAL CONTROL; FLOWER SENESCENCE; SALICYLIC-ACID; RESISTANCE; VIRUS; INFECTION AB The L. regale ATP-binding cassette transporter gene, LrABCF1 belonging to GCN subfamily, functions as a positive regulator of plant defense against Cucumber mosaic virus, Tobacco rattle virus , and Botrytis cinerea in petunia. ATP-binding cassette (ABC) transporters are essential for membrane translocation in diverse biological processes, such as plant development and defense response. Here, a general control non-derepressible (GCN)-type ABC transporter gene, designated LrABCF1, was identified from Cucumber mosaic virus (CMV)-induced cDNA library of L. regale. LrABCF1 was up-regulated upon inoculation with CMV and Lily mottle virus (LMoV). Salicylic acid (SA) and ethylene (ET) application and treatments with abiotic stresses such as cold, high salinity, and wounding increased the transcript abundances of LrABCF1. Constitutive overexpression of LrABCF1 in petunia (Petunia x hybrida) resulted in an impairment of plant growth and development. LrABCF1 overexpression conferred reduced susceptibility to CMV, Tobacco rattle virus (TRV), and B. cinerea infection in transgenic petunia plants, accompanying by elevated transcripts of PhGCN2 and a few defense-related genes in SA-signaling pathway. Our data indicate that LrABCF1 positively modulates viral and fungal resistance. C1 [Sun, Daoyang; Zhang, Xinguo; Li, Shaohua; Zhang, Yanlong; Niu, Lixin] Northwest A&F Univ, Coll Landscape Architecture & Arts, Yangling 712100, Shaanxi, Peoples R China. [Jiang, Cai-Zhong] ARS, USDA, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. [Jiang, Cai-Zhong] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. RP Zhang, YL; Niu, LX (reprint author), Northwest A&F Univ, Coll Landscape Architecture & Arts, Yangling 712100, Shaanxi, Peoples R China.; Jiang, CZ (reprint author), ARS, USDA, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA.; Jiang, CZ (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. EM cjiang@ucdavis.edu; zhangyanlong@nwsuaf.edu.cn; niulixin@nwsuaf.edu.cn FU National High Technology Research and Development Program (863 Program) of China [2011AA100208]; United States Department of Agriculture (USDA) CRIS project [5306-21000-019-00D] FX We thank Min Jin and Xuhong Zhang for experimental assistance in inoculation with B. cinerea. We are grateful to Weixing Shan's favor for conducting some of the experiments in his laboratory. We appreciate Ran Wan for kindly providing B. cinerea strains. This study was partially supported by The National High Technology Research and Development Program (863 Program) of China (Grant no. 2011AA100208) and United States Department of Agriculture (USDA) CRIS project 5306-21000-019-00D. NR 66 TC 0 Z9 0 U1 27 U2 27 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0032-0935 EI 1432-2048 J9 PLANTA JI Planta PD DEC PY 2016 VL 244 IS 6 BP 1185 EP 1199 DI 10.1007/s00425-016-2576-5 PG 15 WC Plant Sciences SC Plant Sciences GA EA1ME UT WOS:000386355600002 PM 27485641 ER PT J AU Motilal, LA Zhang, DP Mischke, S Meinhardt, L Boccara, M Fouet, O Lanaud, C Umaharan, P AF Motilal, Lambert A. Zhang, Dapeng Mischke, Sue Meinhardt, Lyndel W. Boccara, Michel Fouet, Olivier Lanaud, Claire Umaharan, Pathmanathan TI Association mapping of seed and disease resistance traits in Theobroma cacao L. SO PLANTA LA English DT Article DE Black pod disease; Cocoa seeds; Linkage disequilibrium; Microsatellite markers; Single nucleotide polymorphism; Witches' broom disease ID WITCHES-BROOM DISEASE; MARKER-ASSISTED SELECTION; LOCALLY WEIGHTED REGRESSION; EXPRESSED SEQUENCE TAGS; POPULATION-STRUCTURE; MONILIOPHTHORA-PERNICIOSA; LINKAGE DISEQUILIBRIUM; CRINIPELLIS-PERNICIOSA; MORPHOLOGICAL TRAITS; MOLECULAR DIVERSITY AB Microsatellite and single nucleotide polymorphism markers that could be used in marker assisted breeding of cacao were identified for number of filled seeds, black pod resistance and witches' broom disease resistance. An association mapping approach was employed to identify markers for seed number and resistance to black pod and witches' broom disease (WBD) in cacao (Theobroma cacao L.). Ninety-five microsatellites (SSRs) and 775 single nucleotide polymorphisms (SNPs) were assessed on 483 unique trees in the International Cocoa Genebank Trinidad (ICGT). Linkage disequilibrium (LD) and association mapping studies were conducted to identify markers to tag the phenotypic traits. Decay of LD occurred over an average 9.3 cM for chromosomes 1-9 and 2.5 cM for chromosome 10. Marker/trait associations were generally identified based on general linear models (GLMs) that incorporated principal components from molecular information on relatedness factor. Seven markers (mTcCIR 8, 66, 126, 212; TcSNP368, 697, 1370) on chromosomes 1 and 9 were identified for number of filled seeds (NSEED). A single marker was found for black pod resistance (mTcCIR280) on chromosome 3, whereas six markers on chromosomes 4, 5, 6, 8, and 10 were detected for WBD (mTcCIR91, 183; TcSNP375, 720, 1230 and 1374). It is expected that this association mapping study in cacao would contribute to the knowledge of the genetic determinism of cocoa traits and that the markers identified herein would prove useful in marker assisted breeding of cacao. C1 [Motilal, Lambert A.; Boccara, Michel; Umaharan, Pathmanathan] Univ West Indies, Cocoa Res Ctr, Sir Frank Stockdale Bldg, St Augustine 330912, Trinid & Tobago. [Zhang, Dapeng; Mischke, Sue; Meinhardt, Lyndel W.] USDA ARS, Beltsville Agr Res Ctr, PSI, SPCL, 10300 Baltimore Ave,Bldg 001,Rm 223,BARC W, Beltsville, MD 20705 USA. [Boccara, Michel; Fouet, Olivier; Lanaud, Claire] CIRAD UMR AGAP Ctr Cooperat Int Rech Agron Dev, Montpellier 5, France. RP Motilal, LA (reprint author), Univ West Indies, Cocoa Res Ctr, Sir Frank Stockdale Bldg, St Augustine 330912, Trinid & Tobago. EM lambert.motilal@sta.uwi.edu FU Government of Trinidad; Tobago Research Development Fund; UWI-Trinidad and Tobago Research and Development Impact Fund from The University of the West Indies FX This study was partially funded by a grant from the Government of Trinidad and Tobago Research Development Fund and from The UWI-Trinidad and Tobago Research and Development Impact Fund from The University of the West Indies. The use of black pod resistance data from the Cocoa Research Unit (now Cocoa Research Centre) is gratefully acknowledged. The inclusion of some witches' broom disease evaluations from a data set managed by Mrs. Romina Umaharan is gratefully acknowledged. Field evaluations were made possible by several persons including Mr. Leon Ali, Ms. Zainab Ali, Ms. Sarah Bharath, Ms. Rena K. Kalloo, Ms. Carelene Lakhan, Mr. Gangadeen Ramdhanie, Ms. Cassandra Shaw, Mr. Valmiki Singh and Mr. Eusebius Solozano. NR 90 TC 0 Z9 0 U1 13 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0032-0935 EI 1432-2048 J9 PLANTA JI Planta PD DEC PY 2016 VL 244 IS 6 BP 1265 EP 1276 DI 10.1007/s00425-016-2582-7 PG 12 WC Plant Sciences SC Plant Sciences GA EA1ME UT WOS:000386355600008 PM 27534964 ER PT J AU Leeds, TD Vallejo, RL Weber, GM Gonzalez-Pena, D Silverstein, JT AF Leeds, Timothy D. Vallejo, Roger L. Weber, Gregory M. Gonzalez-Pena, Dianelys Silverstein, Jeffrey T. TI Response to five generations of selection for growth performance traits in rainbow trout (Oncorhynchus mykiss) SO AQUACULTURE LA English DT Article DE Heritability; Genetic correlation; Growth; Rainbow trout; Selection ID SALMON SALMO-SALAR; GENETIC-PARAMETERS; BREEDING PROGRAM; ATLANTIC SALMON; BODY-WEIGHT; REPRODUCTIVE TRAITS; FISH; MATURATION; SHELLFISH; GENOTYPE AB A pedigreed rainbow trout population (similar to 100 families per generation) was selected for five generations to improve growth performance to the standard similar to 500-gram US market weight and beyond (>1 kg). Body weights (BW) were recorded each generation at 5, 8, 10, and 13 months post-hatch. Selection was applied to a two-trait index of estimated breeding values for 10-month body weight (i.e., approximate market weight) and thermal growth coefficient between 10 and 13 months, and both traits were given equal weighting in the index. Objectives of this study were to estimate direct selection responses for BW traits and thermal growth coefficient, and correlated response for condition factor traits. Heritability estimates were 0.28 +/- 0.08 to 0.43 +/- 0.14 for BW between 5 and 13 months post-hach, 0.12 +/- 0.03 for thermal growth coefficient, and 0.40 +/- 0.04 and 0.36 +/- 0.04 for condition factor at 10 and 13 months post-hatch, respectively. Genetic correlations among the four BW measures were highest between adjacent BW measures (0.54 +/- 0.19 to 0.82 +/- 0.05) and decreased as time between measures increased. Genetic correlation between thermal growth coefficient and 10-month BW was low (0.18 +/- 0.13), and genetic correlations between condition factor and BW measured at the same age were moderate (0.39 +/- 0.09 to 0.45 +/- 0.08). Compared to randomly-mated control lines, selection response in the selected line averaged 10.6% (5-month BW), 9.8% (8-month BW), 12.7% (10-month BW), 11.9% (13-month BW), 2.1% (thermal growth coefficient), 3.4% (10-month condition factor), and 3.3% (13-month condition factor) per generation. The rate of inbreeding averaged 0.86% per generation in the selection line, with a cumulative inbreeding estimate of 4.5% in the last generation. The low heritability of thermal growth coefficient, and low genetic correlation with 10-month BW, resulted in only modest selection response for thermal growth coefficient. Larger heritabilities of BW measured at fixed ages, and large genetic correlations between serial BW measures, suggest that direct selection on BW will be more favorable than selection on TGC. This study demonstrates the large and sustained potential for genetic improvement of growth performance in domesticated rainbowtroutwith onlymodest accumulation of inbreeding, and providesempirical selection response estimates that have direct inference to commercial breeding programs aiming to select for growth to larger market BW.Statement of relevance: Quantitative genetics data from this relatively long-term and large-scale selective breeding program have direct inference to commercial breeding programs selecting for improved growth, but for which maintenance of control lines is not practical. Published by Elsevier B. V. C1 [Leeds, Timothy D.; Vallejo, Roger L.; Weber, Gregory M.; Gonzalez-Pena, Dianelys; Silverstein, Jeffrey T.] ARS, USDA, Natl Ctr Cool & Cold Water Aquaculture, 11861 Leetown Rd, Kearneysville, WV 25430 USA. [Gonzalez-Pena, Dianelys] Zoetis Anim Genet, Kalamazoo, MI USA. [Silverstein, Jeffrey T.] ARS, USDA, Stoneville, MS USA. RP Leeds, TD (reprint author), ARS, USDA, Natl Ctr Cool & Cold Water Aquaculture, 11861 Leetown Rd, Kearneysville, WV 25430 USA. EM tim.leeds@ars.usda.gov FU U.S. Department of Agriculture (USDA); Agricultural Research Service (ARS) CRIS Project [1930-31000-010]; ORAU under DOE [DE-AC05-06OR23100] FX The authors acknowledge J. Everson, J. Harper, M. Hostuttler, K. Jenkins, J. Kretzer, J. Leasor, J. McGowan, K. Melody, T. Moreland, D. Payne, and E. Squires for technical assistance. This work was supported by the U.S. Department of Agriculture (USDA), Agricultural Research Service (ARS) CRIS Project 1930-31000-010 "Utilizing Genetics and Physiology for Enhancing Cool and Cold Water Aquaculture Production" and in part by an appointment of DGP to the ARS Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and the USDA. ORISE is managed by ORAU under DOE contract number DE-AC05-06OR23100. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the USDA or the ARS of any product or service to the exclusion of others that may be suitable. USDA is an equal opportunity provider and employer. NR 54 TC 0 Z9 0 U1 13 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD DEC 1 PY 2016 VL 465 BP 341 EP 351 DI 10.1016/j.aquaculture.2016.08.036 PG 11 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA DY8IE UT WOS:000385372200041 ER PT J AU Liu, B Storm, DE Zhang, XCJ Cao, WH Duan, XW AF Liu, Bing Storm, Daniel E. Zhang, Xunchang. J. Cao, Wenhong Duan, Xingwu TI A new method for fingerprinting sediment source contributions using distances from discriminant function analysis SO CATENA LA English DT Article DE Sediment fingerprinting; Sediment source; Discriminant function analysis (DFA); Mixing model ID FLUVIAL SUSPENDED SEDIMENT; RIVER-BASINS; URBAN RIVER; CATCHMENT; UNCERTAINTY; MIXTURES; TRACERS; USA AB Mixing models are frequently used as part of sediment fingerprinting to quantify sediment source contributions. Much research effort has been devoted to improving these mixing models. The objective of this study was to develop and evaluate a new method using discriminant function analysis (DFA) to fingerprint sediment source contributions. It was hypothesized that the outcome of DFA, commonly used as a component of standard fingerprinting procedures, can potentially be used directly to quantify source contributions, avoiding mixing models altogether. This hypothesis was tested in the Bull Creek Watershed in Oklahoma State, USA. DFA results were compared with outcomes from the Collins mixing model and previous research. When conservative geochemical tracers were used, DFA results did not differ significantly from the mixing model results, indicating that DFA alone has the potential to accurately quantify sediment source contributions, while being simple to use. When using non-conservative tracers, however, the results from the two methods were significantly different. On the basis of a comparison with previous research, we suggest that DFA offers an intuitive method for characterizing sediment source contributions. (C) 2016 Elsevier B.V. All rights reserved. C1 [Liu, Bing] China Inst Water Resources & Hydropower Res, State Key Lab Simulat & Regulat Water Cycle River, Chegongzhang West St 20, Beijing 10048, Peoples R China. [Storm, Daniel E.] Oklahoma State Univ, Dept Biosyst & Agr Engn, Stillwater, OK 74078 USA. [Zhang, Xunchang. J.] ARS, USDA, Grazinglands Res Lab, El Reno, OK 73036 USA. [Cao, Wenhong] China Inst Water Resources & Hydropower Res, State Key Lab Simulat & Regulat Water Cycle River, Chegongzhang West St 20, Beijing 100048, Peoples R China. [Duan, Xingwu] Yunnan Univ, Inst Int Rivers & Ecosecur, Cuihu North St 2, Kunming 650091, Yunnan, Peoples R China. RP Liu, B (reprint author), China Inst Water Resources & Hydropower Res, State Key Lab Simulat & Regulat Water Cycle River, Chegongzhang West St 20, Beijing 10048, Peoples R China. EM 297101426@qq.com; dan.storm@okstate.edu; John.Zhang@ars.usda.gov; caowh6641@126.com; xwduan@ynu.edu.cn FU Grazinglands Research Lab (El Reno, Oklahoma) [58-6218-0-120]; National Natural Science Foundation of China [41501299]; Non-profit Industry Research Project of the Chinese Ministry of Water Resources [201501045]; Scientific Research Foundation for the Returned Overseas Chinese Scholars FX This project was partially supported by a Specific Cooperative Agreement (58-6218-0-120) from the Grazinglands Research Lab (El Reno, Oklahoma). The study was also supported by the National Natural Science Foundation of China (41501299), the Non-profit Industry Research Project of the Chinese Ministry of Water Resources (201501045) and the Project Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars. The authors are grateful to Dr. Mark E. Payton for statistical advice and Dr. Chad J. Penn for conducting the soil sample tests. NR 38 TC 0 Z9 0 U1 19 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD DEC PY 2016 VL 147 BP 32 EP 39 DI 10.1016/j.catena.2016.06.039 PG 8 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA DZ1LB UT WOS:000385598800004 ER PT J AU Qin, W Guo, QK Zuo, CQ Shan, ZJ Ma, L Sun, G AF Qin, Wei Guo, Qiankun Zuo, Changqing Shan, Zhijie Ma, Liang Sun, Ge TI Spatial distribution and temporal trends of rainfall erosivity in mainland China for 1951-2010 SO CATENA LA English DT Article DE Rainfall erosivity; Spatial distribution and temporal trends; Kendall slope; Mainland China; Soil erosion regions ID CLIMATE-CHANGE; YELLOW-RIVER; NE SPAIN; PRECIPITATION; VARIABILITY; IMPACTS; REGION; MODELS; SCALE; BASIN AB Rainfall erosivity is an important factor for estimating soil erosion rates. Understanding the spatial distribution and temporal trends of rainfall erosivity is especially critical for soil erosion risk assessment and soil conservation planning in mainland China. However, reports on the spatial distribution and temporal trends of rainfall erosivity for China, especially of its eight soil erosion regions, are still lacking, which reduces the accuracy of predicting soil losses, assessing soil erosion risks and evaluating the effects of soil conservation measures. Additionally, the lack of the most suitable spatial interpolation method in mainland China, to some degree, has reduced the applicability and reliability of the interpolation results. In this study, long-term (1951-2010) daily rainfall data from 756 national weather stations were assembled to characterize the spatial and temporal patterns of annual rainfall erosivity across mainland China. Sixteen spatial interpolation methods were compared to select the most suitable one for accurately mapping the spatial distribution of rainfall erosivity, and the Mann-Kendall test was employed to detect the temporal trends. The results indicated that 1) the universal co-kriging method with the aid of elevation was superior to the other spatial interpolation methods; 2) long-term average rainfall erosivity increased from the northwest to the southeast, ranging from 31 to 30,051 MJ mm ha(-1) h(-1) a(-1); 3) overall, rainfall erosivity across China and water erosion regions experienced an insignificant increasing trend over the study period. Significant decreasing trends were observed in the northwest Loess Plateau region (0.01 level), the northeast black soil region and the north earth and gravel mountain region (0.05 level). Significant increasing trends (0.05 level) were found in the southern red soil hilly region and the southwest Karst region; and 4) two lines were identified according to the temporal trends of rainfall erosivity from the east to the west. In total, this study offers useful information both for soil erosion prediction and land management practices of mainland China. (C) 2016 Elsevier B.V. All rights reserved. C1 [Qin, Wei; Guo, Qiankun; Zuo, Changqing; Shan, Zhijie] China Inst Water Resources & Hydropower Res, State Key Lab Simulat & Regulat Water Cycle River, Beijing 100048, Peoples R China. [Qin, Wei; Guo, Qiankun; Zuo, Changqing; Shan, Zhijie] Minist Water Resources, Res Ctr Soil & Water Conservat, Beijing 100048, Peoples R China. [Ma, Liang] Water Resources Res Inst Shandong Prov, Jinan 250013, Shandong, Peoples R China. [Sun, Ge] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Southern Res Stn, USDA, Raleigh, NC 27606 USA. RP Qin, W (reprint author), China Inst Water Resources & Hydropower Res, State Key Lab Simulat & Regulat Water Cycle River, Beijing 100048, Peoples R China.; Qin, W (reprint author), Minist Water Resources, Res Ctr Soil & Water Conservat, Beijing 100048, Peoples R China. EM qinwei@iwhr.com FU Commonwealth Research Program of the Ministry of Water Resources [200901047]; National Basic Research Program of China (973 Program) [2015CB452704]; National Natural Science Foundation of China [31200535] FX This study was funded by the Commonwealth Research Program of the Ministry of Water Resources (No. 200901047), National Basic Research Program of China (973 Program) (No. 2015CB452704) and the National Natural Science Foundation of China (No. 31200535). NR 44 TC 0 Z9 0 U1 30 U2 30 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD DEC PY 2016 VL 147 BP 177 EP 186 DI 10.1016/j.catena.2016.07.006 PG 10 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA DZ1LB UT WOS:000385598800018 ER PT J AU Cadaret, EM McGwire, KC Nouwakpo, SK Weltz, MA Saito, L AF Cadaret, Erik M. McGwire, Kenneth C. Nouwakpo, Sayjro K. Weltz, Mark A. Saito, Laurel TI Vegetation canopy cover effects on sediment erosion processes in the Upper Colorado River Basin Mancos Shale formation, Price, Utah, USA SO CATENA LA English DT Article DE Saline soils; Rangeland Hydrology Erosion Model; Calibration; Vegetation-driven spatial heterogeneity; Landscape metrics; Price River ID SOUTHWESTERN UNITED-STATES; SOIL-EROSION; SOUTHEASTERN ARIZONA; OVERLAND-FLOW; RUNOFF; MODEL; RANGELANDS; RAINFALL; SLOPE; CONTAMINANTS AB This study investigated erosion processes on the highly erosive, saline soils of the Mancos Shale formation in the Price-San Rafael River Basin in Utah, USA. Rainfall simulations were performed at two sites using a Walnut Gulch rainfall simulator with a variety of slope angles and rainfall intensities. The Rangeland Hydrology Erosion Model (RHEM) was calibrated to provide unbiased estimates of discharge and sediment load in runoff at each site. RHEM simulated the inter-plot variability best at the site with higher slope angles, vegetation cover, and sediment loads. The calibrated surface erosion parameters in RHEM (K-ss, K-w) were substantially greater than any published in prior studies from non-saline environments. The spatial distribution of vegetation canopy cover was quantified using photogrammetric modeling and landscape pattern metrics. As the patches of vegetation became more contiguous and the tortuosity of the bare soil area increased, RHEM over-predicted sediment output, suggesting that vegetation-driven spatial heterogeneity influenced erosion in a way that is not captured by the model. (C) 2016 Elsevier B.V. All rights reserved. C1 [Cadaret, Erik M.; McGwire, Kenneth C.] Desert Res Inst, Div Earth & Ecosyst Sci, 2215 Raggio Pkwy, Reno, NV 89512 USA. [Nouwakpo, Sayjro K.; Saito, Laurel] Univ Nevada Reno, Dept Nat Resources & Environm Sci, Reno, NV USA. [Weltz, Mark A.] ARS, USDA, Great Basin Rangelands Res Unit, Reno, NV USA. RP McGwire, KC (reprint author), Desert Res Inst, Div Earth & Ecosyst Sci, 2215 Raggio Pkwy, Reno, NV 89512 USA. EM kenm@dri.edu FU US DOI Bureau of Reclamation/Bureau of Land Management; USDA Agricultural Research Service [59-5370-3-001]; Desert Research Institute of the Nevada System of Higher Education FX Funding for this study was provided by the US DOI Bureau of Reclamation/Bureau of Land Management, USDA Agricultural Research Service (Cooperative Agreement 59-5370-3-001), and the Desert Research Institute of the Nevada System of Higher Education. NR 50 TC 1 Z9 1 U1 23 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD DEC PY 2016 VL 147 BP 334 EP 344 DI 10.1016/j.catena.2016.06.043 PG 11 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA DZ1LB UT WOS:000385598800034 ER PT J AU Cadaret, EM Nouwakpo, SK McGwire, KC Weitz, MA Blank, RR AF Cadaret, Erik M. Nouwakpo, Sayjro K. McGwire, Kenneth C. Weitz, Mark A. Blank, Robert R. TI Experimental investigation of the effect of vegetation on soil, sediment erosion, and salt transport processes in the Upper Colorado River Basin Mancos Shale formation, Price, Utah, USA SO CATENA LA English DT Article DE Saline soils; Rangeland Hydrology and Erosion Model; TDS in runoff; Vegetation driven spatial heterogeneity; Dissolution; Price River ID SOUTHWESTERN UNITED-STATES; SALINITY; PATTERNS; RUNOFF; CONTAMINANTS; SELENIUM AB Because of concerns about salinity in the Colorado River, this study focused on saline and sodic soils associated with the Mancos Shale formation with the objective of investigating mechanisms driving sediment yield and salinity loads and the role of vegetation in altering soil chemistry in the Price-San Rafael River Basin. Rainfall simulations using a Walnut Gulch rainfall simulator were performed at two study sites (Ferron and Price, Utah) across a range of slope angles and rainfall intensities to evaluate the relationship between sediment yield, salinity transport processes, and rainfall-induced changes in soil chemistry. Soil at Ferron had substantially greater salinity than Price as expressed in evaluated sodium absorption ratio, cation exchange capacity in soil, sediment, and total dissolved solids (TDS) in runoff. Principal component analysis and t-tests revealed that the two sites have different runoff and soil chemistry ions. Greater concentrations of K+, NO3-, and Cl- were present in soil under-vegetation microsites compared to interspace soil areas. Soil soluble phase ions generally increased with depth and underwent vertical fluxes at rates proportional to rainfall intensity. Vegetation appears to have a protective effect on the soils from increasing rainfall intensity. Mat-forming saltbush found at Ferron was related most strongly to soil protection. The dissolution of sediment particles in runoff may be a key component of salinity transport processes on the Mancos Shale. Plot-averaged sediment and TDS had a positive linear relationship. The Rangeland Hydrology and Erosion Model successfully predicted TDS in runoff derived from these upland rangelands in central Utah. (C) 2016 Elsevier B.V. All rights reserved. C1 [Cadaret, Erik M.; McGwire, Kenneth C.] Desert Res Inst, Div Earth & Ecosyst Sci, Reno, NV USA. [Nouwakpo, Sayjro K.] Univ Nevada Reno, Dept Nat Resources & Environm Sci, Reno, NV 89557 USA. [Weitz, Mark A.; Blank, Robert R.] ARS, USDA, Great Basin Rangelands Res Unit, Reno, NV USA. RP Nouwakpo, SK (reprint author), Univ Nevada Reno, Dept Nat Resources & Environm Sci, Reno, NV 89557 USA. EM snouwakpo@unr.edu OI Nouwakpo, Sayjro/0000-0002-5138-4169 FU US DOI Bureau of Reclamation, Bureau of Land Management, USDA Agricultural Research Service [59-5370-3-001]; Desert Research Institute; University of Nevada, Reno of the Nevada System of Higher Education FX This study was funded by the US DOI Bureau of Reclamation, Bureau of Land Management, USDA Agricultural Research Service (Cooperative Agreement 59-5370-3-001), in collaboration with the Desert Research Institute and the University of Nevada, Reno of the Nevada System of Higher Education. USDA is an equal opportunity provider and employer. Mention of a proprietary product does not constitute a guarantee or warranty of the product by USDA or the authors, and it does not imply its approval to the exclusion of the other products that also may be suitable. NR 37 TC 0 Z9 0 U1 23 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD DEC PY 2016 VL 147 BP 650 EP 662 DI 10.1016/j.catena.2016.08.024 PG 13 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA DZ1LB UT WOS:000385598800062 ER PT J AU Lindell, CA Steensma, KMM Curtis, PD Boulanger, JR Carroll, JE Burrows, C Lusch, DP Rothwell, NL Wieferich, SL Henrichs, HM Leigh, DK Eaton, RA Linz, GM AF Lindell, Catherine A. Steensma, Karen M. M. Curtis, Paul D. Boulanger, Jason R. Carroll, Juliet E. Burrows, Colleen Lusch, David P. Rothwell, Nikki L. Wieferich, Shayna L. Henrichs, Heidi M. Leigh, Deanna K. Eaton, Rachael A. Linz, George M. TI Proportions of bird damage in tree fruits are higher in low-fruit-abundance contexts SO CROP PROTECTION LA English DT Article DE Apples; Birds; Cherries; Crop damage; Landscape forest cover; Fruit abundance; Tree fruit ID CHERRIES; CONSUMPTION; GRAPES; SWEET AB Frugivorous birds impose significant costs on tree fruit growers through direct consumption of fruit and grower efforts to manage birds. We documented factors that influenced tree fruit bird damage from 2012 through 2014 with a coordinated field study in Michigan, New York, and Washington. For sweet cherries, percent bird damage was higher in 2012 compared to 2013 and 2014, in Michigan and New York compared to Washington, and in blocks with more edges adjacent to non-sweet cherry land-cover types. These patterns appeared to be associated with fruit abundance patterns; 2012 was a particularly low yield year for tree fruits in Michigan and New York and percent bird damage was high. In addition, percent bird damage to sweet and tart cherries in Michigan was higher in landscapes with low to moderate forest cover compared to higher forest cover landscapes. 'Honeycrisp' apple blocks under utility wires were marginally more likely to have greater bird damage compared to blocks without wires. We recommend growers prepare bird management plans that consider the spatial distribution of fruit and non-fruit areas of the farm. Growers should generally expect to invest more in bird management in low-yield years, in blocks isolated from other blocks of the same crop, and in blocks where trees can provide entry to the crop for frugivorous birds. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Lindell, Catherine A.; Eaton, Rachael A.] Michigan State Univ, Dept Integrat Biol, 288 Farm Ln Rm 203, E Lansing, MI 48824 USA. [Lindell, Catherine A.; Wieferich, Shayna L.; Eaton, Rachael A.] Michigan State Univ, Ctr Global Change & Earth Observ, 1405 S Harrison Rd,Manly Miles Bldg, E Lansing, MI 48823 USA. [Lindell, Catherine A.; Eaton, Rachael A.] Michigan State Univ, Ecol Evolutionary Biol & Behav, 103 Giltner Hall,293 Farm Ln Rm 103, E Lansing, MI 48824 USA. [Boulanger, Jason R.] Trinity Western Univ, Dept Biol, 7600 Glover Rd, Langley, BC V2Y 1Y1, Canada. [Curtis, Paul D.; Boulanger, Jason R.; Henrichs, Heidi M.] Cornell Univ, Dept Nat Resources, Room 222 Fernow Hall, Ithaca, NY 14853 USA. [Carroll, Juliet E.] New York State IPM Program, 630 W North St, Geneva, NY 14456 USA. [Burrows, Colleen] Washington State Univ, Whatcom Cty Extens, 1000 N Forest St,Suite 201, Bellingham, WA 98225 USA. [Lusch, David P.] Michigan State Univ, Dept Geog Environm & Spatial Sci, 673 Auditorium Rd Rm 212, E Lansing, MI 48824 USA. [Rothwell, Nikki L.] Michigan State Univ, Northwest Michigan Hort Res Stn, 6686 S Ctr Hwy, Traverse City, MI 49684 USA. [Leigh, Deanna K.] Western Washington Univ, Huxley Coll Environm, 516 High St, Bellingham, WA 98225 USA. [Linz, George M.] USDA APHIS Wildlife Serv Natl Wildlife Res Ctr, 2110 Miriam Circle, Bismarck, ND 58503 USA. [Boulanger, Jason R.] Univ North Dakota, 10 Cornell St,Stop 9019, Grand Forks, ND 58202 USA. [Wieferich, Shayna L.] 919 Terry SL, Golden, CO 80401 USA. [Leigh, Deanna K.] 25766 Lofton Ave, Chisago City, MN 55013 USA. RP Lindell, CA (reprint author), Michigan State Univ, Dept Integrat Biol, 288 Farm Ln Rm 203, E Lansing, MI 48824 USA. EM lindellc@cns.msu.edu FU Specialty Crop Research Initiative of USDA/NIFA [2011-51181-30860]; Cherry Marketing Institute; Michigan Apple Committee; Environment Canada Science Horizons; Ecology, Evolutionary Biology, and Behavior Program at Michigan State University; NSF [IIA-135466]; ND EPSCoR State funds FX This work was supported by the Specialty Crop Research Initiative of USDA/NIFA (Grant number: 2011-51181-30860), the Cherry Marketing Institute, the Michigan Apple Committee, Environment Canada Science Horizons, and the Ecology, Evolutionary Biology, and Behavior Program at Michigan State University. We thank the numerous fruit growers we worked with. Some funding for manuscript preparation came from ND EPSCoR through NSF grant #IIA-135466 and through ND EPSCoR State funds. Funders were not involved with study design, data collection, writing of this report, or decision to submit the article for publication. NR 39 TC 0 Z9 0 U1 7 U2 7 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD DEC PY 2016 VL 90 BP 40 EP 48 DI 10.1016/j.cropro.2016.08.011 PG 9 WC Agronomy SC Agriculture GA DY7OR UT WOS:000385319400006 ER PT J AU Larkin, RP AF Larkin, Robert P. TI Impacts of biocontrol products on Rhizoctonia disease of potato and soil microbial communities, and their persistence in soil SO CROP PROTECTION LA English DT Article DE Potato; Rhizoctonia solani; Trichoderma; Bacillus subtilis; Burkholderia ambifaria; Soil microbial communities ID BURKHOLDERIA-CEPACIA COMPLEX; ESTER FAME PROFILES; FATTY-ACID PROFILES; BIOLOGICAL-CONTROL; SOILBORNE DISEASES; DAMPING-OFF; POPULATION-DYNAMICS; CROPPING SYSTEMS; PLANT-PATHOGENS; BLACK SCURF AB Four commercial biocontrol formulations (Bacillus subtilis GB03, Burkholderia ambifaria type Wisconsin isolate J82, Trichoderma virens GI-21, and Trichoderma harzianum strain T-22), a chemical seed treatment (thiophanate-methyl, mancozeb, and cymoxanil mixture, TMC), and a combination chemical/biological treatment, were compared with no-pathogen and pathogen-treated controls, and monitored in two field seasons in Maine for their effects on the development of Rhizoctonia disease of potato and soil microbial community characteristics. All treatments reduced the incidence and severity of stem canker (37-75% reduction) relative to the pathogen control over both years, with the best control provided by B. subtilis and the combination chemical/biological treatment (TMC/Bamb). Both bacterial treatments (B. subtilis and Bu. ambifaria) reduced severity of black scurf in both years, and T. virens reduced scurf in one year, with reductions of 11-20% relative to the pathogen control. Over both years, the B. subtilis, T virens, and TMC/Bamb treatments increased total and marketable yield, and Bu. ambifaria increased marketable yield, by 11-15% relative to the pathogen control. Substantial populations of the added fungal agents, but not the bacteria, were detected in bulk soil at the end of the growing season. Biocontrol treatments also significantly (P < 0.05) affected soil microbial community characteristics, as assessed by single carbon source substrate utilization (SU) and whole soil fatty acid methyl ester (FAME) profiles. Bacterial biocontrol treatments generally resulted in higher microbial activity and substrate utilization. Some effects on soil microbial communities were also observed the following spring (1 yr after application). This research indicates that biocontrol treatments can assist in the control of Rhizoctonia disease of potato, persist in soil to some degree, and have significant effects on soil microbial communities long after application. Published by Elsevier Ltd. C1 [Larkin, Robert P.] Univ Maine, USDA, ARS, New England Plant Soil & Water Lab, Portage Rd, Orono, ME 04469 USA. RP Larkin, RP (reprint author), Univ Maine, USDA, ARS, New England Plant Soil & Water Lab, Portage Rd, Orono, ME 04469 USA. EM bob.larkin@ars.usda.gov NR 56 TC 0 Z9 0 U1 37 U2 37 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD DEC PY 2016 VL 90 BP 96 EP 105 DI 10.1016/j.cropro.2016.08.012 PG 10 WC Agronomy SC Agriculture GA DY7OR UT WOS:000385319400014 ER PT J AU Sardana, K Bergstrom, JC Bowker, JM AF Sardana, Kavita Bergstrom, John C. Bowker, J. M. TI Valuing setting-based recreation for selected visitors to national forests in the southern United States SO JOURNAL OF ENVIRONMENTAL MANAGEMENT LA English DT Article DE Outdoor recreation; Forest settings; Economic value; Travel costs; Poisson ID RANDOM UTILITY-MODELS; LATENT CLASS APPROACH; ENDOGENOUS STRATIFICATION; COST MODEL; DEMAND; TRUNCATION; REGRESSION; AREAS AB In this study we estimate selected visitors' demand and value for recreational trips to settings such as developed vs. undeveloped sites in U.S. national forests in the Southern United States using the travel cost method. The setting-based approach allows for valuation of multi-activity trips to particular settings. The results from an adjusted Poisson lognormal estimator corrected for truncation and endogenous stratification reveal that economic value per trip estimates are higher for wilderness compared to day use developed settings, overnight-use developed settings, and general forest areas. Estimates of these economic values are important to resource managers because their management decisions and actions typically control recreational settings. For example, managers control developed campground capacity in a national forest, but typically not the number of campers below the capacity constraint and the number and types of activities visitors engage in during a multi-activity trip to a developed campground (within limits since some activities such as discharging a firearm are not permitted in a developed campground). (C) 2016 Elsevier Ltd. All rights reserved. C1 [Sardana, Kavita] TERI Univ, Dept Policy Studies, 10 Inst Area, New Delhi, India. [Bergstrom, John C.] Univ Georgia, Dept Agr & Appl Econ, Athens, GA 30602 USA. [Bowker, J. M.] US Forest Serv, USDA, Southern Res Stn, Washington, DC 20250 USA. RP Sardana, K (reprint author), TERI Univ, Dept Policy Studies, 10 Inst Area, New Delhi, India. EM kavita.sardana@teriuniversity.ac.in NR 42 TC 0 Z9 0 U1 4 U2 4 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0301-4797 EI 1095-8630 J9 J ENVIRON MANAGE JI J. Environ. Manage. PD DEC 1 PY 2016 VL 183 BP 972 EP 979 DI 10.1016/j.jenvman.2016.09.050 PN 3 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA DZ5KC UT WOS:000385900000057 PM 27687633 ER PT J AU Wisniewski, M Droby, S Norelli, J Liu, J Schena, L AF Wisniewski, Michael Droby, Samir Norelli, John Liu, Jia Schena, Leonardo TI Alternative management technologies for postharvest disease control: The journey from simplicity to complexity SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Biological control; Multiple decrement concept; Natural products; Physical treatments ID PATHOGENESIS-RELATED PROTEINS; TREATMENT INDUCES RESISTANCE; GRAPEFRUIT PEEL TISSUE; HOT-WATER TREATMENT; BLUE MOLD DECAY; BIOLOGICAL-CONTROL; PENICILLIUM-EXPANSUM; AUREOBASIDIUM-PULLULANS; SODIUM-BICARBONATE; HEAT-TREATMENT AB Finding safe and effective alternatives to synthetic fungicides for reducing postharvest losses of harvested commodities has been a focus of much research over the past three decades. Identifying alternatives that are widely accepted and commercially viable has, however, been a challenge. The search to identify alternative approaches to postharvest disease management must be viewed in relation to a complex regulatory environment, the need to address disease problems in a wide array of commodities and conditions, industry and consumer acceptance, and last but not least, commercial viability. In order for companies to invest in new technologies, they must be able to clearly see both the value of a product and the financial return on their investment. The present review attempts to highlight how the search for alternative postharvest disease management technologies has been a journey from simplicity to complexity. Plant pathologists developing alternative technologies are slowly moving away from the "silver bullet" concept where a single intervention can be used to control a disease to viewing plant disease as a process where multiple interventions may be required at different points in the disease process. Over the past thirty years, alternatives have moved from the simple idea of applying high concentrations of biocontrol agents to a harvested commodity, to using a wide array of other alternatives, and integrating them together into a systems approach based on the multiple decrement or multiple hurdle concept. The ease of sequencing genomes and obtaining related genotypic, transcriptomic, proteomic, and metabolomic information is leading to the development of new commercial technologies where problems are solved "biologically." Published by Elsevier B.V. C1 [Wisniewski, Michael; Norelli, John] USDA ARS, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. [Droby, Samir] Volcani Ctr, Dept Postharvest Sci Fresh Produce ARO, POB 6, IL-50250 Bet Dagan, Israel. [Liu, Jia] Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei 230009, Peoples R China. [Schena, Leonardo] Univ Mediterranea Reggio Calabria, Dipartimento Agr, I-89124 Reggio Di Calabria, Italy. RP Wisniewski, M (reprint author), USDA ARS, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM michael.wisniewski@ars.usda.gov; samird@volcani.agri.gov.il; jay.norelli@ars.usda.gov; liujia@hfut.edu.cn; lschena@unirc.it FU U.S. Israel Binational Agricultural Research and Development Fund [US-4774-14C] FX The current review was not designed to be comprehensive and inclusive of the many hundreds of reports that have been published on this topic. Rather, it was designed to provide a 'historical' view of how the field has developed over the past thirty years and the ideas and concepts that helped to shape it. We would like to extend our gratitude to all those researchers, mentioned and unmentioned, who have diligently worked to find and develop safe and effective alternatives for the management of postharvest diseases. In many traditions, the earth was originally perceived as a "Garden of Eden." As the activities of humans, both in industry and population growth, begin to represent a real challenge to the health and sustainability of the planet, let us hope that science and ethical conviction can provide a safe and bountiful harvest for all the people of future generations. A portion of the research described in this review was partially funded by a grant (US-4774-14C) from the U.S. Israel Binational Agricultural Research and Development Fund. NR 107 TC 2 Z9 2 U1 32 U2 32 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD DEC PY 2016 VL 122 SI SI BP 3 EP 10 DI 10.1016/j.postharvbio.2016.05.012 PG 8 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA DY9RP UT WOS:000385473100002 ER PT J AU Droby, S Wisniewski, M Teixido, N Spadaro, D Jijakli, MH AF Droby, Samir Wisniewski, Michael Teixido, Neus Spadaro, Davide Jijakli, M. Haissam TI The science, development, and commercialization of postharvest biocontrol products SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Antagonists; Biological control; Biopesticide; Microbiome; Postharvest ID AGENT CANDIDA-SAKE; INDIGENOUS MICROBIAL COMMUNITY; INITIAL CELL CONCENTRATION; PANTOEA-AGGLOMERANS CPA-2; PEPPER PLANT PHYLLOSPHERE; EPICOCCUM-NIGRUM CONIDIA; BIOLOGICAL-CONTROL AGENT; BROWN-ROT DISEASE; SODIUM-BICARBONATE; PENICILLIUM-DIGITATUM AB Postharvest biological control agents as a viable alternative to the use of synthetic chemicals have been the focus of considerable research for the last 30 years by many scientists and several commercial companies worldwide. Several antagonists of postharvest pathogens have been identified and tested in laboratory, semi-commercial, and commercial settings and were developed into commercial products. The discovery and development of these antagonists into a product followed a paradigm in which a single antagonist isolated from one commodity was also expected to be effective on other commodities that vary in their genetic background, physiology, postharvest handling, and susceptibility to pathogens. In most cases, product development was successfully achieved but their full commercial potential was not realized. The low success rate of postharvest biocontrol products has been attributed to several problems, including difficulties in mass production and formulation of the antagonist, the physiological status of the harvested commodity and its susceptibility to specific pathogens. All these factors played a major role in the reduced and inconsistent performance of the biocontrol product when used under commercial conditions. Although many studies have been conducted on the mode of action of postharvest microbial antagonists, our understanding is still very incomplete. In this regard, a systems approach, that takes into account all the components of the biocontrol system, may represent the best approach to investigating the network of interactions that exist. Very little is known about the overall diversity and composition of microbial communities on harvested produce and how these communities vary across produce types, their function, the factors that influence the composition of the microbiota after harvest and during storage, and the distribution of individual taxa. In light of the progress made in recent years in metagenomic technologies, this technology should be used to characterize the composition of microbial communities on fruit and vegetables. Information on the dynamics and diversity of microbiota may be useful to developing a new paradigm in postharvest biocontrol that is based on constructing synthetic microbial communities that provide superior control of pathogens. (C) 2016 Elsevier B.V. All rights reserved. C1 [Droby, Samir] Volcani Ctr, ARO, Inst Postharvest & Food Sci, Dept Postharvest Sci, POB 6, IL-50250 Bet Dagan, Israel. [Wisniewski, Michael] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Teixido, Neus] XaRTA Postharvest, IRTA, Edifici FRUITCTR, Lleida 25003, Catalonia, Spain. [Spadaro, Davide] Univ Torino, Dept Agr Forestry & Food Sci DISAFA, Largo Braccini 2, I-10095 Grugliasco, TO, Italy. [Spadaro, Davide] Univ Torino, AGROINNOVA Ctr Competence, Innovat Agroenvironm Sect, Largo Braccini 2, I-10095 Grugliasco, TO, Italy. [Jijakli, M. Haissam] ULg, Gembloux Agrobio Tech, Integrated & Urban Plant Pathol Lab, Passage Deportes,2, B-5030 Gembloux, Belgium. RP Droby, S (reprint author), Volcani Ctr, ARO, Inst Postharvest & Food Sci, Dept Postharvest Sci, POB 6, IL-50250 Bet Dagan, Israel. EM samird@volcani.agri.gov.il RI Teixido, Neus/D-2487-2017; Spadaro, Davide/F-1994-2011 OI Teixido, Neus/0000-0002-1676-3593; Spadaro, Davide/0000-0001-5207-9345 NR 95 TC 4 Z9 4 U1 45 U2 45 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD DEC PY 2016 VL 122 SI SI BP 22 EP 29 DI 10.1016/j.postharvbio.2016.04.006 PG 8 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA DY9RP UT WOS:000385473100004 ER PT J AU Haddix, ML Magrini-Bair, K Evans, RJ Conant, RT Wallenstein, MD Morris, SJ Calderon, F Paul, EA AF Haddix, Michelle L. Magrini-Bair, Kim Evans, Robert J. Conant, Richard T. Wallenstein, Matthew D. Morris, Sherri J. Calderon, Francisco Paul, Eldor A. TI Progressing towards more quantitative analytical pyrolysis of soil organic matter using molecular beam mass spectroscopy of whole soils and added standards SO GEODERMA LA English DT Article DE Analytical pyrolysis; Molecular beam mass spectroscopy; Soil organic matter chemistry; Pyrolysis standards ID PARTICLE-SIZE FRACTIONS; SPECTROMETRY PY-MBMS; C-13 CPMAS NMR; K-EDGE XANES; HUMIC SUBSTANCES; CHEMICAL-COMPOSITION; FOREST SOILS; TEMPERATURE SENSITIVITY; AGRICULTURAL SOILS; GC-MS AB Soil organic matter (SOM) is extremely complex. It is composed of hundreds of different organic substances and it has been difficult to quantify these diverse substances in a dynamic-ecosystem functioning standpoint. Analytical pyrolysis has been used to compare chemical differences between soils, but its ability to measure the absolute amount of a specific compound in the soil is still in question. Our objective was to assess whether utilizing pyrolysis -molecular beam mass spectroscopy (py-MBMS) to define the signature of known reference compounds (adenine, indole, palmitic add, etc. ) and biological samples (chitin, fungi, cellulose, etc. ) separately and when added to whole soils it was possible to make py-MBMS more quantitative. Reference compounds, spanning a wide variety of compound categories, and biological samples, expected to be present in SOM, were added to three soils from Colorado, Ohio, and Massachusetts that have varying total C, % clay, and clay type. Py-MBMS, a rapid analysis technique originally developed to analyze complex biomolecules, flash pyrolyzes soil organic matter to form products that are often considered characteristic of the original molecular structure. Samples were pyrolyzed at 550 C by py-MBMS. All samples were weighed and %C and %N determined both before and after pyrolysis to evaluate mass loss, C loss, and N loss for the samples. An average relationship of r(2) = 0. 76 (P = 0. 005) was found for the amount of cellulose added to soil at 25, 50, and 100% of soil C relative to the ion intensity of select mass/charge of the compound. There was a relationship of r(2) = 0. 93 (P<0.001) for the amount of indole added to soil at 25, 50, and 100% of soil C and the ion intensity of the associated mass variables (mass/charge). Comparing spectra of pure compounds with the spectra of the compounds added to soil and isolated clay showed that interference could occur based on soil type and compound with the Massachusetts soil with high C (55.8 g C kg(-1)) and low % clay (5.4%) having the least interference and the Colorado soil with low C (14.6 g C kg(-1)) and a moderate smectite day content of 14% having the greatest soil interference. Due to soil interference from clay type and content and varying optimum temperatures of pyrolysis for different compounds it is unlikely that analytical pyrolysis can be quantitative for all types of compounds. Select compound categories such as carbohydrates have the potential to be quantified in soil with analytical pyrolysis due to the fact that they: 1) almost fully pyrolyzed, 2) were represented by a limited number of m/z, and 3) had a strong relationship with the amount added and the total ion intensity produced. The three different soils utilized in this study had similar proportions of C pyrolyzed in the whole soil (54-57%) despite differences in %C and %clay between the soils. Mid-infrared spectroscopic analyses of the soil before and after pyrolysis showed that pyrolysis resulted in reductions in the 3400, 29302870, 1660 and 1430 cm(-1) bands. These bands are primarily representative of O-H and N-H bonds, C-H stretch, and delta (CH2) in polysaccharides/lipid and are associated with mineralizable SOM. The incorporation of standards into routine analytical pyrolysis allowed us to assess the quantitative potential of py-MBMS along with the effect of the mineral matrix, which we believe is applicable to all forms of analytical pyrolysis. (C) 2016 Elsevier B.V. All rights reserved. C1 [Haddix, Michelle L.; Conant, Richard T.; Wallenstein, Matthew D.; Paul, Eldor A.] Colorado State Univ, Nat Resource Ecol Lab, 200 West Lake St, Ft Collins, CO 80523 USA. [Magrini-Bair, Kim] Natl Renewable Energy Lab, 15013 Denver West Pkwy, Golden, CO 80401 USA. [Evans, Robert J.] MicroChem Technol Inc, 8999 W Harvard Pl, Lakewood, CO 80227 USA. [Morris, Sherri J.] Bradley Univ, Dept Biol, 1501 W Bradley Ave, Peoria, IL 61625 USA. [Calderon, Francisco] USDA ARS, Cent Great Plains Res Stn, Akron, CO 80720 USA. [Paul, Eldor A.] Colorado State Univ, Dept Soil & Crop Sci, 200 West Lake St, Ft Collins, CO 80523 USA. RP Haddix, ML (reprint author), Colorado State Univ, Nat Resource Ecol Lab, 200 West Lake St, Ft Collins, CO 80523 USA. EM michelle.haddix@colostate.edu FU National Science Foundation Division of Environmental Biology [0842315]; Office of Science (BER), U.S. Department of Energy FX We would like to thank Sean Maloney for his assistance with sample preparation, Robert Sykes for his assistance with instrument operation, Dr. Dukes for the use of soil from his experimental site, Dr. Jessica Ernakovich for her review of this manuscript, and the valuable insights of two anonymous reviewers. This research was supported by the National Science Foundation Division of Environmental Biology under grant number 0842315 and the Office of Science (BER), U.S. Department of Energy. NR 70 TC 0 Z9 0 U1 28 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD DEC 1 PY 2016 VL 283 BP 88 EP 100 DI 10.1016/j.geoderma.2016.07.027 PG 13 WC Soil Science SC Agriculture GA DV9XZ UT WOS:000383296800010 ER PT J AU Tang, SK Avena-Bustillos, RJ Lear, M Sedej, I Holstege, DM Friedman, M McHugh, TH Wang, SC AF Tang, Shuaikun Avena-Bustillos, Roberto J. Lear, Molly Sedej, Ivana Holstege, Dirk M. Friedman, Mendel McHugh, Tara H. Wang, Selina C. TI Evaluation of thermal processing variables for reducing acrylamide in canned black ripe olives SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Acrylamide reduction; Black ripe olives; Thermal processing; LACF lethality ID FOODS; MITIGATION; TOXICITY AB Acrylamide, formed in certain plant foods at elevated cooking temperatures, has been identified as a probable carcinogen. A wide variation in acrylamide concentration has been reported for commercial canned black ripe olives, with some levels being quite high. Standards for olive sterilization were developed before acrylamide was discovered to be present in food. The objective of this study was to determine if alternative sterilization conditions during thermal processing could substantially reduce acrylamide levels in black ripe olives, while still maintaining safety and quality. Heat penetration tests for six thermal processes were used to evaluate process F-0 - a sterilization value which was correlated to acrylamide formation and changes in quality attributes of black ripe olives. Acrylamide concentration followed a positively correlated second order polynomial regression with process F-0. This correlation was further demonstrated by two of the experimental processes with different initial temperatures and different processing times, but with ultimately similar F-0, that produced very similar levels of acrylamide. The amounts of solids leaching from olives in brine increased at higher temperatures of thermal processing, whereas pH variation in olives and brine was most likely related to the differences in lye-wash cycles. Skin color was unaffected, whereas increasing (time/temperature) thermal processing reduced the firmness of whole olives. Optimization of safe thermal processing conditions while lowering process F-0 is a practical and efficient strategy to reduce acrylamide formation and improve the safety of black ripe olives. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Tang, Shuaikun; Lear, Molly; Wang, Selina C.] Univ Calif Davis, Dept Food Sci & Technol, One Shields Ave, Davis, CA 95616 USA. [Avena-Bustillos, Roberto J.; Friedman, Mendel; McHugh, Tara H.] ARS, USDA, Western Reg Res Lab, Hlth Processed Foods Res, 800 Buchanan St, Albany, CA 94710 USA. [Sedej, Ivana; Wang, Selina C.] Univ Calif Davis, Olive Ctr, One Shields Ave, Davis, CA 95616 USA. [Holstege, Dirk M.] Univ Calif Davis, Univ Calif Analyt Lab, One Shields Ave, Davis, CA 95616 USA. RP Wang, SC (reprint author), Univ Calif Davis, Dept Food Sci & Technol, One Shields Ave, Davis, CA 95616 USA.; Avena-Bustillos, RJ (reprint author), ARS, USDA, Western Reg Res Lab, Hlth Processed Foods Res, 800 Buchanan St, Albany, CA 94710 USA. EM scwang@ucdavis.edu OI Friedman, Mendel/0000-0003-2582-7517 FU California Olive Commission FX Funding was provided by the California Olive Commission. NR 25 TC 0 Z9 0 U1 16 U2 16 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 EI 1873-5770 J9 J FOOD ENG JI J. Food Eng. PD DEC PY 2016 VL 191 BP 124 EP 130 DI 10.1016/j.jfoodeng.2016.07.011 PG 7 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA DV9YG UT WOS:000383297500014 ER PT J AU Juneja, VK Cadavez, V Gonzales-Barron, U Mukhopadhyay, S Friedman, M AF Juneja, Vijay K. Cadavez, Vasco Gonzales-Barron, Ursula Mukhopadhyay, Sudarsan Friedman, Mendel TI Effect of pomegranate powder on the heat inactivation of Escherichia coli O104:H4 in ground chicken SO FOOD CONTROL LA English DT Article DE Escherichia coli; Minced chicken; Weibull; Polynomial; Predictive model; Thermal lethality ID PUNICA-GRANATUM; LISTERIA-MONOCYTOGENES; ANTIMICROBIAL ACTIVITY; THERMAL INACTIVATION; HETEROCYCLIC AMINES; BEEF PATTIES; ANTIBACTERIAL ACTIVITY; STAPHYLOCOCCUS-AUREUS; POLYPHENOL EXTRACTS; SODIUM-CHLORIDE AB Health concerns have led to a search for natural plant-based antimicrobials. Ellagic acid has been shown to have antimicrobial activity against foodborne pathogens. The objective of this study was to assess the effect of a high-ellagic acid commercial pomegranate on the heat resistance of Escherichia coli O104:H4 in ground chicken. A full 24 factorial design was used, consisting of temperature treatment with four levels (55.0, 57.5, 60.0, and 62.5 degrees C) and pomegranate with four levels (0.0,1.0, 2.0, and 3.0 wt/wt. % containing 70% ellagic acid). Experiments were conducted twice, providing a total of 32 survival curves. A three parameter Weibull primary model was used to describe survival kinetics. Secondary models were then developed to estimate the shape parameter beta (i.e., curvature representing susceptibility of cells to stress), scale parameter gamma (i.e., time to reach the first decimal reduction) and the 5.0-log lethality time t(5.0) (i.e., time to reach a 5.0-log reduction), all as polynomial functions of temperature and pomegranate powder concentration. The positive effect of pomegranate concentration on both beta and gamma demonstrated that the phenolic-rich pomegranate powder causes E. coli O104:H4 cells to become more susceptible to heat, increasing the steepness and concavity of the isothermal survival curves. It was estimated that the 5.0-log reduction time would reach a minimum at a pomegranate powder concentration of 1%, producing a 50% decrease in lethality time, in comparison to that without added pomegranate powder. Nonetheless, a mixed-effect omnibus regression further confirmed that the greatest difference in the thermal resistance of E. coli O104:H4 happened between tests with and without pomegranate powder. In fact, adding more than 1.0% pomegranate powder, at a constant temperature, resulted only in a marginal decrease in thermal resistance. Meat processors can use the model to design lethality treatments in order to achieve specific reductions of E. coli O104:H4 in ground chicken. (C) 2016 Published by Elsevier Ltd. C1 [Juneja, Vijay K.; Mukhopadhyay, Sudarsan] USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Cadavez, Vasco; Gonzales-Barron, Ursula] CIMO Mt Res Ctr, Polytech Inst Braganza, Sch Agr, Braganza, Portugal. [Friedman, Mendel] USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. RP Juneja, VK (reprint author), USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM vijay.juneja@ars.usda.gov OI Friedman, Mendel/0000-0003-2582-7517 FU Portuguese Foundation for Science and Technology (FCT) [IF/00570] FX We thank Angie Osoria and Carol E. Levin for technical assistance as well as in facilitating the preparation of the manuscript. Also, Dr. Gonzales-Barron wishes to acknowledge the financial support provided by the Portuguese Foundation for Science and Technology (FCT) through the award of a five-year Investigator Fellowship (IF) in the mode of Development Grants (IF/00570). NR 38 TC 0 Z9 0 U1 39 U2 39 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD DEC PY 2016 VL 70 BP 26 EP 34 DI 10.1016/j.foodcont.2016.05.027 PG 9 WC Food Science & Technology SC Food Science & Technology GA DT5RI UT WOS:000381539900004 ER PT J AU Yi, J Fan, YT Zhang, YZ Wen, Z Zhao, LQ Lu, YJ AF Yi, Jiang Fan, Yuting Zhang, Yuzhu Wen, Zhen Zhao, Liqing Lu, Yujuan TI Glycosylated alpha-lactalbumin-based nanocomplex for curcumin: Physicochemical stability and DPPH-scavenging activity SO FOOD HYDROCOLLOIDS LA English DT Article DE alpha-lactalbumin; Nanoencapsulation; Curcumin; Glycosylation; Antioxidant activity ID SOY PROTEIN ISOLATE; BETA-LACTOGLOBULIN; MAILLARD REACTION; ANTIOXIDANT ACTIVITY; EMULSION STABILITY; WATER-SOLUBILITY; CAROTENE; PRODUCTS; CELLS; FOOD AB Low stability at high salt concentrations, iso-electric point, and high temperature restricted the application of proteins as stabilizers in nutraceutical encapsulation. Protein-polysaccharide conjugates made with Maillard reactions may be better alternatives. In this study, the characteristics of curcumin nano-complexes prepared with both a-lactalbumin (ALA) and ALA-dextran conjugates as well as the effects of encapsulations on the physicochemical stability and the antioxidant activity of curcumin were evaluated. Curcumin aqueous dispersibility was boosted with nanoencapsulation. The sizes of nanoparticles coated with ALA and ALA-dextran conjugates were 63.5 +/- 1.2 and 68.1 +/- 2.9, respectively. Encapsulation efficiency (EE) and loading amount (LA) was not influenced with glycosylation. Stability under environmental stresses has significantly improved with ALA-dextran conjugates, compared to ALA. The oxidative stability of curcumin was also remarkably increased, especially with ALA-dextran conjugates at 95 degrees C. Curcumin coated in ALA and ALA-dextran conjugates had higher DPPH-scavenging activity, compared to free curcumin. ALA-dextran conjugates based nanocarrier may be an excellent delivery system for curcumin to expand the application in a wide range of food products. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Yi, Jiang; Wen, Zhen; Zhao, Liqing; Lu, Yujuan] Shenzhen Univ, Coll Chem & Environm Engn, Shenzhen 518060, Peoples R China. [Fan, Yuting; Zhang, Yuzhu] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Lu, YJ (reprint author), Shenzhen Univ, Coll Chem & Environm Engn, Shenzhen 518060, Peoples R China. EM yjlv@szu.edu.cn FU Natural Science Foundation of China [21276160]; Natural Science Foundation of Guangdong Province [2015A030313540]; Shenzhen Dedicated Funding of Strategic Emerging Industry Development Program [JCYJ20140418091413576, JCYJ20150525092941062, CXZZ20150430093131635] FX This work was supported by the Natural Science Foundation of China (No. 21276160), the Natural Science Foundation of Guangdong Province (No. 2015A030313540), and the Shenzhen Dedicated Funding of Strategic Emerging Industry Development Program (JCYJ20140418091413576, JCYJ20150525092941062, CXZZ20150430093131635). NR 44 TC 1 Z9 1 U1 61 U2 61 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0268-005X EI 1873-7137 J9 FOOD HYDROCOLLOID JI Food Hydrocolloids PD DEC PY 2016 VL 61 BP 369 EP 377 DI 10.1016/j.foodhyd.2016.05.036 PG 9 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DU5LO UT WOS:000382253400041 ER PT J AU Hong, YK Huang, LH Yoon, WB Liu, F Tang, JM AF Hong, Yoon-Ki Huang, Lihan Yoon, Won Byong Liu, Fang Tang, Juming TI Mathematical modeling and Monte Carlo simulation of thermal inactivation of non-proteolytic Clostridium botulinum spores during continuous microwave-assisted pasteurization SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Microwave-assisted pasteurization system (MAPS); Probabilistic modeling; Lethality; Clostridium botulinum spores; Monte Carlo simulation ID FOODS; STERILIZATION; TEMPERATURE; SAFETY AB The objective of this study was to develop a mathematical method to predict the internal temperature history of products exposed to a microwave-assisted pasteurization system and use Monte Carlo simulation toanalyze the inactivation of the spores of Clostridium botulinum Types B in beef meatball trays and Type E in salmon fillet trays. With a target of 6 log-reduction in the spores, the simulation showed >98.8% and 99.1% of the processes will achieve a minimum of 5-log reductions of C botulinum Type B in beef meatball trays and Type E in salmon fillet trays, respectively. Sensitivity analysis showed that the heating temperature, time, and product heating rate in the Microwave-Assisted Heating section and the heating temperature in the Pre-Heating section are four most critical factors affecting the accumulation of lethality. The results of this study may be used to guide the development of more effective thermal processes in microwave-assisted pasteurization systems. Published by Elsevier Ltd. C1 [Hong, Yoon-Ki; Yoon, Won Byong] Kangwon Natl Univ, Coll Agr & Life Sci, Dept Food Sci & Biotechnol, Chunchon 200701, Gangwon Do, South Korea. [Huang, Lihan] ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Yoon, Won Byong] Kangwon Natl Univ, Agr & Life Sci Res Inst, Chunchon 200701, Gangwon Do, South Korea. [Liu, Fang; Tang, Juming] Washington State Univ, Dept Biol Syst Engn, Pullman, WA 99164 USA. RP Huang, LH (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM lihan.huang@ars.usda.gov OI Huang, Lihan/0000-0002-9565-4503 NR 25 TC 0 Z9 0 U1 35 U2 35 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 EI 1873-5770 J9 J FOOD ENG JI J. Food Eng. PD DEC PY 2016 VL 190 BP 61 EP 71 DI 10.1016/j.jfoodeng.2016.06.012 PG 11 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA DU6UE UT WOS:000382349300008 ER PT J AU Wang, JM Zhuang, H Hinton, A Zhang, JH AF Wang, Jiamei Zhuang, Hong Hinton, Arthur, Jr. Zhang, Jianhao TI Influence of in-package cold plasma treatment on microbiological shelf life and appearance of fresh chicken breast fillets SO FOOD MICROBIOLOGY LA English DT Article DE Poultry; Microbial; L*; Mesophiles; Psychrophiles; Pseudomonas ID ATMOSPHERIC-PRESSURE PLASMA; SALMONELLA-ENTERICA; SPOILAGE BACTERIA; ESCHERICHIA-COLI; LISTERIA-INNOCUA; MEAT; INACTIVATION; POULTRY; QUALITY; PORK AB The effect of in-package cold plasmas (CP) was studied on microbiological shelf life and surface lightness of fresh chicken fillets (pectoralis major). Chicken fillets were packaged in food trays in air or modified atmosphere (MA) gas (O-2: CO2: N-2 = 65: 30: 5) and stored at 4 degrees C after exposed to an in-package cold plasma (80 kV for 180 s) treatment. Populations of mesophiles, psychrophiles, and pseudomonas spp. were measured as indicators for microbiological shelf life and CIELAB L* values as an indicator for raw meat appearance. Results show that regardless of microbial type, there were no significant differences in microbial counts between the control and CP treated chicken fillets packed in air. However, in the MA packages, microbial counts were consistently lower than the non-treated control during refrigerated storage. Regardless of CP treatment, the microbial counts on the samples packed in air were much higher than in MA. They were more than 6 logs cfu/g in air compared to fewer than 4 logs cfu/g in MA after 7 d storage and fewer than 6 logs cfu/g after 14 d storage. Regardless of CP treatment and gas composition in package, there were no significant differences in the surface L* value between the fillets pre-treatment and those after storage at 4 degrees C. These results demonstrate that the effects of in-package CP treatments on microbiological shelf life of fresh chicken fillets depend upon headspace composition in packages. When fresh chicken fillets are packed in air, CP treatment has no effect on microbiological shelf life. MA packages with high O-2 and CO2 significantly extend shelf life and CP treatment with MA can at least double shelf life of fresh chicken meat (more than 14 days). Regardless of headspace composition, in-package CP does not have negative effects on chicken meat appearance. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Wang, Jiamei] Hainan Univ, Coll Food Sci & Technol, Haikou 570228, Peoples R China. [Wang, Jiamei; Zhang, Jianhao] Nanjing Agr Univ, Natl Ctr Meat Qual & Safety Control, Nanjing 210095, Jiangsu, Peoples R China. [Zhuang, Hong; Hinton, Arthur, Jr.] ARS, USDA, Athens, GA 30605 USA. RP Zhang, JH (reprint author), Nanjing Agr Univ, Coll Food Sci & Technol, 1 Weigang, Nanjing 210095, Jiangsu, Peoples R China. EM nau_zjh@njau.edu.cn FU National science and technology support program [2015BAD16B05-5]; Agriculture science and technology innovation fund of Jiangsu province [CX(15)1049]; International cooperation project of Jiangsu province [BZ2014034]; Scientific research funding of Hainan University [kyqd 1560] FX The research leading to these results has received funding from National science and technology support program (2015BAD16B05-5), Agriculture science and technology innovation fund of Jiangsu province (CX(15)1049), International cooperation project of Jiangsu province (BZ2014034) and Scientific research funding of Hainan University (kyqd 1560). NR 26 TC 0 Z9 0 U1 46 U2 62 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0740-0020 EI 1095-9998 J9 FOOD MICROBIOL JI Food Microbiol. PD DEC PY 2016 VL 60 BP 142 EP 146 DI 10.1016/j.fm.2016.07.007 PG 5 WC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology SC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology GA DT7OW UT WOS:000381677100017 PM 27554156 ER PT J AU Aguilera, Y Mojica, L Rebollo-Hernanz, M Berhow, M de Mejia, EG Martin-Cabrejas, MA AF Aguilera, Yolanda Mojica, Luis Rebollo-Hernanz, Miguel Berhow, Mark de Mejia, Elvira Gonzalez Martin-Cabrejas, Maria A. TI Black bean coats: New source of anthocyanins stabilized by beta-cyclodextrin copigmentation in a sport beverage SO FOOD CHEMISTRY LA English DT Article DE Anthocyanins; Black bean; beta-Cyclodextrin; Copigmentation; Natural colorant; Sport beverage ID ANTIOXIDANT CAPACITY STABILITY; GRAPE SKIN ANTHOCYANINS; PHASEOLUS-VULGARIS L.; IN-VITRO INHIBITION; DEGRADATION KINETICS; ASCORBIC-ACID; INTERMOLECULAR COPIGMENTATION; PHENOLIC COMPOSITION; THERMAL-STABILITY; ALPHA-GLUCOSIDASE AB Anthocyanin-rich powders and aqueous extracts, with high antioxidant activities, were obtained from black bean seed coats and applied to colour a sport beverage. Idaho and Otomi bean coats were extracted in water-citric acid 2% (1/50, w/v), stirring for 4 h at 40 circle C. Anthocyanins from Idaho and Otomi extracts (1.83 mg and 1.02 mg C3G/g, respectively) were applied to a commercially available sport beverage, with and without 2% beta-cyclodextrin (beta CD) under light and darkness conditions for 10 days, and stored at 4 circle C and 25 circle C for 6 weeks. At different light and storage conditions, anthocyanin degradation fitted a first-order reaction model. All bean coat anthocyanins combined with beta CD showed extended half-life (up to 13 months), higher D-values (up to 43 months) and fewer differences in colourimetric properties (lightness, chroma and hue angle) under darkness and 4 circle C conditions. These black bean coat aqueous extracts and powders might represent natural alternatives to synthetic colorants, ecologically extracted, and with a high antioxidant potential. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Aguilera, Yolanda; Rebollo-Hernanz, Miguel; Martin-Cabrejas, Maria A.] Univ Autonoma Madrid, Fac Ciencias, Inst Invest Ciencias Alimentac, E-28049 Madrid, Spain. [Mojica, Luis; de Mejia, Elvira Gonzalez] Univ Illinois, Dept Food Sci & Human Nutr, Urbana, IL 61801 USA. [Berhow, Mark] ARS, USDA, 1815 N Univ St, Peoria, IL 61604 USA. RP Martin-Cabrejas, MA (reprint author), Univ Autonoma Madrid, Fac Ciencias, Inst Invest Ciencias Alimentac, E-28049 Madrid, Spain. EM maria.martin@uam.es RI Rebollo-Hernanz, Miguel/L-9523-2015 OI Rebollo-Hernanz, Miguel/0000-0003-1034-1290 NR 42 TC 2 Z9 2 U1 36 U2 102 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD DEC 1 PY 2016 VL 212 BP 561 EP 570 DI 10.1016/j.foodchem.2016.06.022 PG 10 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA DP8OP UT WOS:000378757800070 PM 27374568 ER PT J AU Arnade, C Vocke, G AF Arnade, Carlos Vocke, Gary TI Seasonal Variation in the Price Discovery Process of International Wheat SO AGRIBUSINESS LA English DT Article ID MARKETS; COMPONENTS; CAUSALITY; SECURITY; GRAPHS AB This paper uses the Gonzalo-Granger price discovery method to determine whether wheat prices are primarily discovered in the Northern Hemisphere or Southern Hemisphere and whether the price discovery process changes throughout the calendar year. Testing U.S. hard red winter prices against Argentina's wheat price we find that the United States dominates the price discovery process 7 months of the year. Testing U.S Portland soft white prices against Australia's wheat price we find that both countries play a significant role in price discovery but the relative country contribution to price formation varies throughout the year. We also find that the price discovery process changes over time. (C) 2015 Wiley Periodicals, Inc. C1 [Arnade, Carlos; Vocke, Gary] USDA, Econ Res Serv, Englewood, CO USA. RP Arnade, C; Vocke, G (reprint author), USDA, Econ Res Serv, Englewood, CO USA. EM Carnade@ers.usda.gov; Gvocke@ers.usda.gov NR 30 TC 0 Z9 0 U1 18 U2 43 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0742-4477 EI 1520-6297 J9 AGRIBUSINESS JI Agribusiness PD WIN PY 2016 VL 32 IS 1 BP 16 EP 32 DI 10.1002/agr.21428 PG 17 WC Agricultural Economics & Policy; Economics; Food Science & Technology SC Agriculture; Business & Economics; Food Science & Technology GA DJ5HM UT WOS:000374237500002 ER PT J AU Palmer, MV Thacker, TC Waters, WR AF Palmer, Mitchell V. Thacker, Tyler C. Waters, W. Ray TI Differential Cytokine Gene Expression in Granulomas from Lungs and Lymph Nodes of Cattle Experimentally Infected with Aerosolized Mycobacterium bovis SO PLOS ONE LA English DT Article ID IN-SITU HYBRIDIZATION; NECROSIS-FACTOR-ALPHA; CALMETTE-GUERIN; MESSENGER-RNA; TUBERCULOSIS; MICE; PROGRESSION; DELTA-RD1; RELEVANCE; PATHOLOGY AB The hallmark lesion of tuberculosis in humans and animals is the granuloma. The granuloma represents a distinct host cellular immune response composed of epithelioid macrophages, lymphocytes, and multinucleated giant cells, often surrounding a caseous necrotic core. Within the granuloma, host-pathogen interactions determine disease outcome. Factors within the granulomas such as cytokines and chemokines drive cell recruitment, activity, function and ultimately the success or failure of the host's ability to control infection. Hence, an understanding of the granuloma-level cytokine response is necessary to understand tuberculosis pathogenesis. In-situ cytokine expression patterns were measured using a novel in situ hybridization assay, known as RNAScope (R) in granulomas of the lungs, tracheobronchial lymph nodes and caudal mediastinal lymph nodes of cattle experimentally infected with Mycobacterium bovis via aerosol exposure. In spite of microscopic morphological similarities, significant differences were seen between late stage granulomas of the lung compared to those of the tracheobronchial lymph nodes for IL-17A, IFN-gamma, TGF-beta, IL10 and IL22 but not for TNF-alpha. Additionally, significant differences were noted between granulomas from two different thoracic lymph nodes that both receive afferent lymphatics from the lungs (i.e., tracheobronchial and caudal mediastinal lymph nodes) for TNF-alpha, IL-17A, IFN-gamma, TGF-beta and IL-10 but not for IL-22. These findings show that granuloma morphology alone is not a reliable indicator of granuloma function as granulomas of similar morphologies can have disparate cytokine expression patterns. Moreover, anatomically distinct lymph nodes (tracheobronchial vs caudal mediastinal) differ in cytokine expression patterns even when both receive afferent lymphatics from a lung containing tuberculoid granulomas. These findings show that selection of tissue and anatomic location are critical factors in assessing host immune response to M. bovis and should be considered carefully. C1 [Palmer, Mitchell V.; Thacker, Tyler C.; Waters, W. Ray] ARS, Infect Bacterial Dis Livestock Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50011 USA. RP Palmer, MV (reprint author), ARS, Infect Bacterial Dis Livestock Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50011 USA. EM mitchell.palmer@ars.usda.gov FU US Department of Agriculture, Agricultural Research Service Project (CRIS) [3625-32000-104] FX Research funds were provided by the US Department of Agriculture, Agricultural Research Service Project (CRIS# 3625-32000-104). NR 39 TC 0 Z9 0 U1 4 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 30 PY 2016 VL 11 IS 11 AR e0167471 DI 10.1371/journal.pone.0167471 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EE3FV UT WOS:000389474100085 PM 27902779 ER PT J AU Taning, CNT Andrade, EC Hunter, WB Christiaens, O Smagghe, G AF Taning, Clauvis N. T. Andrade, Eduardo C. Hunter, Wayne B. Christiaens, Olivier Smagghe, Guy TI Asian Citrus Psyllid RNAi Pathway - RNAi evidence SO Scientific Reports LA English DT Article ID DOUBLE-STRANDED-RNA; RECEPTOR-MEDIATED ENDOCYTOSIS; SYSTEMIC RNAI; INTERFERENCE DIRECTS; GREENING DISEASE; C. ELEGANS; DROSOPHILA; PROTEINS; GENES; DSRNA AB Diaphorina citri, known as the Asian citrus psyllid, is an important pest of citrus because it transmits a phloem-limited bacteria strongly implicated in huanglongbing (citrus greening disease). Emerging biotechnologies, such as RNA interference, could provide a new sustainable and environmentally friendly strategy for the management of this pest. In this study, genome and functional analysis were performed to verify whether the RNAi core genes are present in the Asian psyllid genome and if the RNAi machinery could be exploited to develop a management strategy for this pest. Analyses of RNAi-related genes in the Asian citrus psyllid genome showed an absence of sequences encoding R2D2, a dsRNA-binding protein that functions as a cofactor of Dicer-2 in Drosophila. Nevertheless, bioassays using an in Planta System showed that the Asian citrus psyllid was very sensitive to ingested dsRNA, demonstrating a strong RNAi response. A small dose of dsRNA administered through a citrus flush was enough to trigger the RNAi mechanism, causing significant suppression of the targeted transcript, and increased psyllid mortality. This study provides evidence of a functional RNAi machinery, which could be further exploited to develop RNAi based management strategies for the control of the Asian citrus psyllid. C1 [Taning, Clauvis N. T.; Christiaens, Olivier; Smagghe, Guy] Univ Ghent, Fac Biosci Engn, Dept Crop Protect, B-9000 Ghent, Belgium. [Andrade, Eduardo C.] EMBRAPA Cassava & Fruits, Rua Embrapa,S-N, BR-44380000 Cruz Das Almas, Bahia, Brazil. [Hunter, Wayne B.] ARS, USDA, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. RP Taning, CNT (reprint author), Univ Ghent, Fac Biosci Engn, Dept Crop Protect, B-9000 Ghent, Belgium. EM tiziclauvis.taningnji@ugent.be FU Kansas State University; USDA, NIFA, Citrus Greening award [2015-70016-23028]; NIFA GRANT [2014-10154]; Citrus Research Board, CRB; Florida Citrus Production Research Advisory Council; Los Alamos National Lab; Biosciences Division, Group B-1, LANL, NM FX We thank Dr. Xiomara Sinisterra-Hunter, AgTec, LLC, Plant Biotechnology Consultant, Port St. Lucie, FL, for critical reviews of the manuscript and data analysis, and Maria Gonzalez, USDA, Biological Science Technician, Fort Pierce, FL, for technical assistance. International Psyllid Genome Consortium, Asian Citrus Psyllid, Diaphorina citri. DIACI 2010-2015: Analytical, bioinformatics: Reese Consulting, LLC; Dr. Adelaide Rhodes, Center for Genome Res. And Biocomputing, Oregon State Unviersity, OR, Drs. Stephen Richards, Adam English, Baylor Human Genome Centre, HGC, Houston TX; Susan Brown, KSU, Surya Saha, BTI, Monica Munoz-Torres, NCBI. NIFA GRANT Awarded, National Institute of Food and Agriculture. #2014-10154. Project Director: Kansas State University, Grant : Developing an Infrastructure and Product Test Pipeline to Deliver Novel Therapies for Citrus Greening Disease". Funding in-part from: USDA, NIFA, Citrus Greening award #2015-70016-23028, Susan Brown, Surya Saha, Monica Munoz-Torres; Citrus Research Board, CRB, 2011, Psyllid Genome, $96,450; Florida Citrus Production Research Advisory Council, 2008, Psyllid genomics and citrus greening interactions, $120,000; Los Alamos National Lab, Drs. Shannon Johnson, and Shunsheng Han, Biosciences Division, Group B-1, LANL, NM 2011-2012. USDA-ARS, Subtropical Insects Research Unit, Fort Pierce, FL, $68,000, 2010-2011. Brazilian Agricultural Research Corporation (EMBRAPA), Labex program. Ghent University, Special Research Fund and Fund for Scientific Research, Flanders 2015. Mention of trade names or commercial products herein is solely for the purpose of providing specific information and does not imply recommendation or endorsement, to the exclusion of other similar products or services by the U.S. Department of Agriculture and Ghent University. USDA and Ghent University are equal opportunity providers and employers. NR 73 TC 0 Z9 0 U1 17 U2 17 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD NOV 30 PY 2016 VL 6 AR 38082 DI 10.1038/srep38082 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EE1NE UT WOS:000389348000001 PM 27901078 ER PT J AU Arp, AP Hunter, WB Pelz-Stelinski, KS AF Arp, Alex P. Hunter, Wayne B. Pelz-Stelinski, Kirsten S. TI Annotation of the Asian Citrus Psyllid Genome Reveals a Reduced Innate Immune System SO FRONTIERS IN PHYSIOLOGY LA English DT Article DE Diaphorina citri; immune; genes; Imd; liberibacter; antimicrobial peptide; virus ID PEPTIDOGLYCAN-RECOGNITION PROTEIN; FUMOSOROSEA HYPOCREALES CORDYCIPITACEAE; CANDIDATUS LIBERIBACTER ASIATICUS; GRAM-POSITIVE BACTERIA; MOSQUITO AEDES-AEGYPTI; JAK-STAT PATHWAY; ANOPHELES-GAMBIAE; DIAPHORINA-CITRI; DROSOPHILA TOLL; MANDUCA-SEXTA AB Citrus production worldwide is currently facing significant losses due to citrus greening disease, also known as Huanglongbing. The citrus greening bacteria, Candidatus Liberibacter asiaticus (CLas), is a persistent propagative pathogen transmitted by the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae). Hemipterans characterized to date lack a number of insect immune genes, including those associated with the lmd pathway targeting Gram-negative bacteria. The a citri draft genome was used to characterize the immune defense genes present in D. citri. Predicted mRNAs identified by screening the published D. citri annotated draft genome were manually searched using a custom database of immune genes from previously annotated insect genomes. Toll and JAK/STAT pathways, general defense genes Dual oxidase, Nitric oxide synthase, prophenoloxidase, and cellular immune defense genes were present in a citri. In contrast, D. citri lacked genes for the lmd pathway, most antimicrobial peptides, 1,3-beta-glucan recognition proteins (GNBPs), and complete peptidoglycan recognition proteins. These data suggest that a citri has a reduced immune capability similar to that observed in A. pisurn, P. humanus, and R. prolixus. The absence of immune system genes from the D. citri genome may facilitate CLas infections, and is possibly compensated for by their relationship with their microbial endosymbionts. C1 [Arp, Alex P.; Pelz-Stelinski, Kirsten S.] Univ Florida, Dept Entomol & Nematol, Ctr Agr Res & Educ, Ft Pierce, FL 34945 USA. [Hunter, Wayne B.] ARS, US Hort Res Lab, USDA, Ft Pierce, FL USA. RP Pelz-Stelinski, KS (reprint author), Univ Florida, Dept Entomol & Nematol, Ctr Agr Res & Educ, Ft Pierce, FL 34945 USA. EM pelzstelinski@ufl.edu FU Florida Citrus Production Research Advisory Council FX The Florida Citrus Research and Development Foundation, Inc, 2011 (Project # 05-021). Supported by grants from: USDA-ARS 2010-2011. The Florida Citrus Production Research Advisory Council, 2008. The BioScience Division, Los Alamos National Laboratory, NM, 2011-2012. The Citrus Research Board, 2012. NR 167 TC 1 Z9 1 U1 9 U2 9 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-042X J9 FRONT PHYSIOL JI Front. Physiol. PD NOV 30 PY 2016 VL 7 AR 570 DI 10.3389/fphys.2016.00570 PG 18 WC Physiology SC Physiology GA ED5RC UT WOS:000388909000001 PM 27965582 ER PT J AU Nimmakayala, P Abburi, VL Saminathan, T Alaparthi, SB Almeida, A Davenport, B Nadimi, M Davidson, J Tonapi, K Yadav, L Malkaram, S Vajja, G Hankins, G Harris, R Park, M Choi, D Stommel, J Reddy, UK AF Nimmakayala, Padma Abburi, Venkata L. Saminathan, Thangasamy Alaparthi, Suresh B. Almeida, Aldo Davenport, Brittany Nadimi, Marjan Davidson, Joshua Tonapi, Krittika Yadav, Lav Malkaram, Sridhar Vajja, Gopinath Hankins, Gerald Harris, Robert Park, Minkyu Choi, Doil Stommel, John Reddy, Umesh K. TI Genome-wide Diversity and Association Mapping for Capsaicinoids and Fruit Weight in Capsicum annuum L SO SCIENTIFIC REPORTS LA English DT Article ID POPULATION-STRUCTURE; PROVIDES INSIGHTS; SHOOT MERISTEM; TOMATO; PEPPER; QTLS; SIZE; DOMESTICATION; COLLECTION; GENES AB Accumulated capsaicinoid content and increased fruit size are traits resulting from Capsicum annuum domestication. In this study, we used a diverse collection of C. annuum to generate 66,960 SNPs using genotyping by sequencing. The study identified 1189 haplotypes containing 3413 SNPs. Length of individual linkage disequilibrium (LD) blocks varied along chromosomes, with regions of high and low LD interspersed with an average LD of 139 kb. Principal component analysis (PCA), Bayesian model based population structure analysis and an Euclidean tree built based on identity by state (IBS) indices revealed that the clustering pattern of diverse accessions are in agreement with capsaicin content (CA) and fruit weight (FW) classifications indicating the importance of these traits in shaping modern pepper genome. PCA and IBS were used in a mixed linear model of capsaicin and dihydrocapsaicin content and fruit weight to reduce spurious associations because of confounding effects of subpopulations in genome-wide association study (GWAS). Our GWAS results showed SNPs in Ankyrin-like protein, IKI3 family protein, ABC transporter G family and pentatricopeptide repeat protein are the major markers for capsaicinoids and of 16 SNPs strongly associated with FW in both years of the study, 7 are located in known fruit weight controlling genes. C1 [Nimmakayala, Padma; Abburi, Venkata L.; Saminathan, Thangasamy; Alaparthi, Suresh B.; Almeida, Aldo; Davenport, Brittany; Nadimi, Marjan; Davidson, Joshua; Tonapi, Krittika; Yadav, Lav; Malkaram, Sridhar; Vajja, Gopinath; Hankins, Gerald; Harris, Robert; Reddy, Umesh K.] West Virginia State Univ, Gus R Douglass Inst, Institute, WV 25112 USA. [Nimmakayala, Padma; Abburi, Venkata L.; Saminathan, Thangasamy; Alaparthi, Suresh B.; Almeida, Aldo; Davenport, Brittany; Nadimi, Marjan; Davidson, Joshua; Tonapi, Krittika; Yadav, Lav; Malkaram, Sridhar; Vajja, Gopinath; Hankins, Gerald; Harris, Robert; Reddy, Umesh K.] West Virginia State Univ, Dept Biol, Institute, WV 25112 USA. [Park, Minkyu; Choi, Doil] Seoul Natl Univ, Plant Genom & Breeding Inst, Dept Plant Sci, Coll Agr & Life Sci, Seoul 151321, South Korea. [Stommel, John] ARS, Genet Improvement Fruits & Vegetables Lab, USDA, Beltsville, MD 20705 USA. RP Reddy, UK (reprint author), West Virginia State Univ, Gus R Douglass Inst, Institute, WV 25112 USA.; Reddy, UK (reprint author), West Virginia State Univ, Dept Biol, Institute, WV 25112 USA. EM ureddy@wvstateu.edu RI Choi, Doil/F-5002-2011 FU USDA-NIFA [2010-02419]; NSF-EPSCOR [09-570 - EPS-1003907]; NIH [P20RR016477]; Agricultural Genome Center of Next Generation Biogreen 21 program, RDA of Korean government [PJ011275-01]; Gus R. Douglass Institute FX The study received funding from USDA-NIFA (2010-02419), NSF-EPSCOR#09-570 - EPS-1003907, NIH Grant P20RR016477 to the West Virginia IDeA Network for Biomedical Research Funding and the Gus R. Douglass Institute (graduate research assistantship to V.A., K.T., L.Y. and B.D.). Doil Choi was supported by the Agricultural Genome Center of Next Generation Biogreen 21 program (PJ011275-01), RDA of Korean government. NR 55 TC 0 Z9 0 U1 8 U2 8 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD NOV 30 PY 2016 VL 6 AR 38081 DI 10.1038/srep38081 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA ED7IZ UT WOS:000389038700001 PM 27901114 ER PT J AU Ma, L Ashworth, D Yates, SR AF Ma, Li Ashworth, Daniel Yates, Scott R. TI Simultaneous determination of estrogens and progestogens in honey using high performance liquid chromatography-tandem mass spectrometry SO JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS LA English DT Article DE Estrogens; Progestogens; LC-MS/MS; Solid phase extraction ID CHEMICAL-COMPOSITION; BISPHENOL-A; ANTIBIOTICS; HORMONE; MS AB This work describes the development and validation of a method for the simultaneous determination of 13 estrogens and progestogens in honey by high performance liquid chromatography-tandem mass spectrometry. The hormones were preconcentrated by solid phase extraction. Pretreatment variables were optimized for a better compatibility with electrospray ionization interfaced mass spectrometry. The analytes were analyzed in multiple-reaction monitoring mode with two pairs of precursor product ion transitions. The proposed method was validated with method detection limits of 0.01-0.33 ng/g and good linearities (r(2) > 0.9901) throughout the studied concentration range. The recoveries of analytes at the spiking levels (5 ng/g and 25 ng/g) ranged from 71.2% to 99.7%, with relative standard deviations below 20%. The method was used to determine the target compounds in honey samples (orange blossom, clover and multiflower) obtained from supermarkets. Two samples of honey were found to contain trace amounts of estrone (30-week-old broiler breeders. The mean bird lethal dose (BLD50) was 5.0 log(10) mean egg infectious dose (EID50) for all age groups. The mean death time (MDT) was statistically not different among the three age groups, ranging between 3.2 and 4.8 days. All broilers that became infected shed high levels of virus with transmission to contacts and demonstrated severe pathology. Mortality and virus shedding results indicated that age is not a determinant factor in susceptibility of broilers to H5N2 clade 2.3.4.4 HPAI virus. Previously, the Tk/MN/15 virus had a BLD50 of 3.6 log(10) EID50 and MDT of 2 days in White Leghorn chickens and a BLD50 of 5.0 log(10) EID50 and MDT of 5.9 days in turkeys, suggesting that the broiler breed is less susceptible to Midwestern H5N2 virus than the layer breed but similarly susceptible to turkeys. Therefore, genetic resistance of broilers to infection may have accounted only partially for the lack of affected broiler farms in the Midwestern outbreaks, with other contributing factors such as fewer outside to on farm exposure to contacts, type of production management system or enhanced biosecurity. C1 [Bertran, Kateri; Lee, Dong-Hun; Balzli, Charles; Pantin-Jackwood, Mary J.; Spackman, Erica; Swayne, David E.] ARS, Exot & Emerging Avian Viral Dis Res Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr,USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. RP Swayne, DE (reprint author), ARS, Exot & Emerging Avian Viral Dis Res Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr,USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. EM david.swayne@ars.usda.gov FU USDA CRIS [6040-32000-063-00D] FX This work was funded by USDA CRIS Project 6040-32000-063-00D. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the USDA. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. NR 44 TC 0 Z9 0 U1 4 U2 4 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0928-4249 EI 1297-9716 J9 VET RES JI Vet. Res. PD NOV 21 PY 2016 VL 47 AR 116 DI 10.1186/s13567-016-0401-6 PG 11 WC Veterinary Sciences SC Veterinary Sciences GA EC5BV UT WOS:000388149000001 PM 27871330 ER PT J AU Gerber, PF Xiao, CT Lager, K Crawford, K Kulshreshtha, V Cao, DJ Meng, XJ Opriessnig, T AF Gerber, Priscilla F. Xiao, Chao-Ting Lager, Kelly Crawford, Kimberly Kulshreshtha, Vikas Cao, Dianjun Meng, Xiang-Jin Opriessnig, Tanja TI Increased frequency of porcine epidemic diarrhea virus shedding and lesions in suckling pigs compared to nursery pigs and protective immunity in nursery pigs after homologous re-challenge SO VETERINARY RESEARCH LA English DT Article ID EXPERIMENTAL-INFECTION; ANTIBODY-RESPONSES; NURSING PIGLETS; UNITED-STATES; WEANED PIGS; PEDV; COLOSTRUM; VIRULENT; MUCOSAL; TISSUES AB Porcine epidemic diarrhea virus (PEDV) causes enteric disease in pigs and spreads rapidly after entering naive pig populations. The objectives were to (1) compare the disease course following inoculation with PEDV isolate US/Colorado/2013 in naive 10 day and 8 week-old pigs, and (2) contrast the naive response to homologous challenge in 8 week-old pigs. Pigs were randomly assigned into group 1 (n = 40, no PEDV exposure), group 2 (n = 43, PEDV inoculation at 10 days of age) and group 3 (n = 48, PEDV inoculation at 8 weeks of age). Thirty-three group 2 pigs received a homologous challenge at 8 weeks of age. Following primary or secondary inoculation, 3-10 pigs were euthanized at days post-inoculation (dpi) 1, 2, 3, 7 or 14. Clinical signs were more pronounced in 10 day-old pigs compared to 8 week-old pigs at dpi 2 and 3, a higher number of 10 day-old pigs shed PEDV RNA in feces compared to 8 week-old pigs. Typical severe atrophic enteritis of PEDV infection was observed at dpi 3 in both age groups, and at dpi 4 and 14 fecal shedding patterns were also similar. While both age groups had seroconverted to PEDV by dpi 14, IgG levels were higher in 8 week-old pigs. PEDV IgA antibodies were detected in feces of approximately 50% of the pigs at dpi 44. In homologous challenged pigs, no clinical signs or lesions were found, and PEDV fecal shedding was restricted to less than 10% of the pigs indicating the existence of homologous protection 44 days after initial PEDV exposure. C1 [Gerber, Priscilla F.; Opriessnig, Tanja] Univ Edinburgh, Roslin Inst, Midlothian, Scotland. [Gerber, Priscilla F.; Opriessnig, Tanja] Univ Edinburgh, Royal Dick Sch Vet Studies, Midlothian, Scotland. [Xiao, Chao-Ting; Opriessnig, Tanja] Iowa State Univ, Dept Vet Diagnost & Prod Anim Med, Ames, IA USA. [Xiao, Chao-Ting] Hunan Univ, Coll Biol, Changsha, Hunan, Peoples R China. [Lager, Kelly; Crawford, Kimberly; Kulshreshtha, Vikas] ARS, Natl Anim Dis Ctr, USDA, Ames, IA USA. [Cao, Dianjun; Meng, Xiang-Jin] Virginia Polytech Inst & State Univ, Coll Vet Med, Dept Biomed Sci & Pathobiol, Blacksburg, VA 24061 USA. RP Opriessnig, T (reprint author), Univ Edinburgh, Roslin Inst, Midlothian, Scotland.; Opriessnig, T (reprint author), Univ Edinburgh, Royal Dick Sch Vet Studies, Midlothian, Scotland. EM Tanja.Opriessnig@roslin.ed.ac.uk FU Elanco Animal Health; Biotechnology and Biological Sciences Research Council (BBSRC) Institute Strategic Programme Grant [BB/J004324/1, BBS/E/D/20241864]; USDA-ARS; USDA-APHIS Interagency Agreement [14-9419-340] FX Support for this study was provided in part by Elanco Animal Health (XM, TO), the Biotechnology and Biological Sciences Research Council (BBSRC) Institute Strategic Programme Grant awarded to the Roslin Institute (BB/J004324/1; BBS/E/D/20241864) (PG, TO), USDA-ARS, and a USDA-APHIS Interagency Agreement 14-9419-340 (KL, KC, VK). NR 23 TC 0 Z9 0 U1 4 U2 4 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0928-4249 EI 1297-9716 J9 VET RES JI Vet. Res. PD NOV 21 PY 2016 VL 47 AR 118 DI 10.1186/s13567-016-0402-5 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA EC7XI UT WOS:000388354000002 PM 27871312 ER PT J AU Min, SC Roh, SH Niemira, BA Sites, JE Boyd, G Lacombe, A AF Min, Sea C. Roh, Si Hyeon Niemira, Brendan A. Sites, Joseph E. Boyd, Glenn Lacombe, Alison TI Dielectric barrier discharge atmospheric cold plasma inhibits Escherichia coli O157:H7, Salmonella, Listeria monocytogenes, and Tulane virus in Romaine lettuce SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Cold plasma; E. coli O157:H7; Salmonella; L. monocytogenes; Tulane virus; Romaine lettuce ID ENTERICA SEROVAR TYPHIMURIUM; FRESH PRODUCE; PATHOGEN INACTIVATION; HUMAN NOROVIRUS; OXYGEN PLASMA; PRESSURE; STERILIZATION; DECONTAMINATION; MICROORGANISMS; BIOFILMS AB The present study investigated the effects of dielectric barrier discharge atmospheric cold plasma (DACP) treatment on the inactivation of Escherichia coli O157:H7, Salmonella, Listeria monocytogenes, and Tulane virus (TV) on Romaine lettuce, assessing the influences of moisture vaporization, modified atmospheric packaging (MAP), and post-treatment storage on the inactivation of these pathogens. Romaine lettuce was inoculated with E. coli O157:H7, Salmonella, L. monocytogenes (similar to 6 log CFU/g lettuce), or TV (similar to 2 log PFU/g lettuce) and packaged in either a Petri dish (diameter: 150 mm, height: 15 mm) or a Nylon/polyethylene pouch (152 x 254 mm) with and without moisture vaporization. Additionally, a subset of pouch-packaged leaves was flushed with O-2 at 5% or 10% (balance N-2). All of the packaged lettuce samples were treated with DACP at 34.8 kV for 5 min and then analyzed either immediately or following post-treatment storage for 24 h at 4 degrees C to assess the inhibition of microorganisms. DACP treatment inhibited E. coli O157:H7, Salmonella, L. monocytogenes, and TV by 1.1 +/- 0.4, 0.4 +/- 03, 1.0 +/- 0.5 log CFU/g, and 13 +/- 0.1 log PFU/g, respectively, without environmental modifications of moisture or gas in the packages. The inhibition of the bacteria was not significantly affected by packaging type or moisture vaporization (p > 0.05) but a reduced-oxygen MAP gas composition attenuated the inhibition rates of E. coli O157:H7 and TV. L. monocytogenes continued to decline by an additional 0.6 log CFU/g in post-treatment cold storage for 24 h. Additionally, both rigid and flexible conventional plastic packages appear to be suitable for the in-package decontamination of lettuce with DACP. Published by Elsevier B.V. C1 [Min, Sea C.; Roh, Si Hyeon; Niemira, Brendan A.; Sites, Joseph E.; Boyd, Glenn] USDA, Eastern Reg Res Ctr, 600 Mermaid Lane, Wyndmoor, PA 19038 USA. [Min, Sea C.; Roh, Si Hyeon] Seoul Womens Univ, Dept Food Sci & Technol, 621 Hwarangro, Seoul 139774, South Korea. [Lacombe, Alison] Natl Coll Nat Med, 014 SE Porter St, Portland, OR 97201 USA. RP Niemira, BA (reprint author), USDA, Eastern Reg Res Ctr, 600 Mermaid Lane, Wyndmoor, PA 19038 USA. EM Brendan.Niemira@ars.usda.gov FU High Value-added Food Technology Development Program, Ministry of Agriculture, Food and Rural Affairs (MAFRA) [314059-3] FX Author Min was supported by High Value-added Food Technology Development Program (314059-3), Ministry of Agriculture, Food and Rural Affairs (MAFRA). The authors wish to acknowledge the technical assistance of Breanna Tyrell and Melissa Fleck. NR 43 TC 1 Z9 1 U1 15 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD NOV 21 PY 2016 VL 237 BP 114 EP 120 DI 10.1016/j.ijfoodmicro.2016.08.025 PG 7 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA EA2FN UT WOS:000386408300014 PM 27562348 ER PT J AU Zhou, L He, H Li, MC Song, KL Cheng, HN Wu, QL AF Zhou, Ling He, Hui Li, Mei-Chun Song, Kunlin Cheng, H. N. Wu, Qinglin TI Morphological influence of cellulose nanoparticles (CNs) from cottonseed hulls on rheological properties of polyvinyl alcohol/CN suspensions SO CARBOHYDRATE POLYMERS LA English DT Article DE Cottonseed hull; Cellulose nanocrystals; Morphology; Polyvinyl alcohol; Rheological behavior ID NANOCRYSTALLINE CELLULOSE; AQUEOUS SUSPENSIONS; BY-PRODUCTS; SOY HULLS; NANOFIBERS; BEHAVIORS; FIBERS; FILMS AB The present work describes the isolation of cellulose nanoparticles (CNs) with different morphologies and their influence on rheological properties of CN and CN-poly (vinyl alcohol) (PVA) suspensions. Cottonseed hulls were used for the first time to extract three types of CNs, including fibrous cellulose nanofibers, rod -like cellulose nanocrystals and spherical cellulose nanoparticles through mechanical and chemical methods. Rheology results showed that the rheological behavior of the CN suspensions was strongly dependent on CN concentration and particle morphology. For PVA/CN systems, concentration of PVA/CN suspension, morphology of CNs, and weight ratio of CN to PVA were three main factors that influenced their rheology behaviors. This research reveals the importance of CN morphology and composition concentration on the rheological properties of PVA/CN, providing new insight in preparing high performance hydrogels, fibers and films base on PVA/CN suspension systems. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Zhou, Ling; He, Hui] South China Univ Technol, Sch Mat Sci & Engn, Guangzhou 510640, Guangdong, Peoples R China. [Zhou, Ling; Li, Mei-Chun; Song, Kunlin; Wu, Qinglin] Louisiana State Univ, AgCtr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. [Cheng, H. N.] USDA ARS, Southern Reg Res Ctr, New Orleans, LA 70124 USA. RP He, H (reprint author), South China Univ Technol, Sch Mat Sci & Engn, Guangzhou 510640, Guangdong, Peoples R China.; Wu, QL (reprint author), Louisiana State Univ, AgCtr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. EM pshuihe@scut.edu.cn; qwusfm@gmail.com RI Song, Kunlin/B-2285-2017; Li, Meichun/F-7815-2015 OI Song, Kunlin/0000-0003-2141-1020; Li, Meichun/0000-0001-9381-0093 FU Science and Technology Project of Guangzhou [201508020090]; USDA National Institute of Food and Agriculture McIntire Stennis project [1000017]; China Scholarship Council (CSC) FX The work is supported by the Science and Technology Project of Guangzhou [201508020090], the USDA National Institute of Food and Agriculture McIntire Stennis project [1000017] and China Scholarship Council (CSC). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 30 TC 0 Z9 0 U1 27 U2 31 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD NOV 20 PY 2016 VL 153 BP 445 EP 454 DI 10.1016/j.carbpol.2016.07.119 PG 10 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA DU6IF UT WOS:000382317200052 PM 27561516 ER PT J AU Grote, J Krysciak, D Petersen, K Gullert, S Schmeisser, C Forstner, KU Krishnan, HB Schwalbe, H Kubatova, N Streit, WR AF Grote, Jessica Krysciak, Dagmar Petersen, Katrin Guellert, Simon Schmeisser, Christel Foerstner, Konrad U. Krishnan, Hari B. Schwalbe, Harald Kubatova, Nina Streit, Wolfgang R. TI The Absence of the N-acyl-homoserine-lactone Autoinducer Synthase Genes tral and ngrl Increases the Copy Number of the Symbiotic Plasmid in Sinorhizobium fredii NGR234 SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE Sinorhizobium fredii; plasmid copy number; plant symbioses; quorum sensing (OS); RNA sequencing (RNA-Seq) ID SP STRAIN NGR234; NITROGEN-FIXING RHIZOBIA; SECRETION SYSTEMS; REPLICATION GENES; NODULATION GENES; REPABC OPERON; NOD GENES; MELILOTI; EXPRESSION; USDA257 AB Plant-released flavonoids induce the transcription of symbiotic genes in rhizobia and one of the first bacterial responses is the synthesis of so called Nod factors. They are responsible for the initial root hair curling during onset of root nodule development. This signal exchange is believed to be essential for initiating the plant symbiosis with rhizobia affiliated with the Alphaproteobacteria. Here, we provide evidence that in the broad host range strain Sinorhizobium fredii NGR234 the complete lack of quorum sensing molecules results in an elevated copy number of its symbiotic plasmid (pNGR234a). This in turn triggers the expression of symbiotic genes and the production of Nod factors in the absence of plant signals. Therefore, increasing the copy number of specific plasmids could be a widespread mechanism of specialized bacterial populations to bridge gaps in signaling cascades. C1 [Grote, Jessica; Krysciak, Dagmar; Petersen, Katrin; Guellert, Simon; Schmeisser, Christel; Streit, Wolfgang R.] Univ Hamburg, Dept Microbiol & Biotechnol, Bioctr Klein Flottbek, Hamburg, Germany. [Foerstner, Konrad U.] Univ Wurzburg, Core Unit Syst Med, Wurzburg, Germany. [Krishnan, Hari B.] Univ Missouri, Plant Genet Res Unit, ARS, USDA, Columbia, MO USA. [Schwalbe, Harald; Kubatova, Nina] Goethe Univ Frankfurt, Ctr Biomol Magnet Resonance, Inst Organ Chem & Chem Biol, Frankfurt, Germany. RP Streit, WR (reprint author), Univ Hamburg, Dept Microbiol & Biotechnol, Bioctr Klein Flottbek, Hamburg, Germany. EM wolfgang.streit@uni-hamburg.de FU Deutsche Forschungsgemeinschaft [STR451/7-1, SPP1617] FX This work was kindly funded by the Deutsche Forschungsgemeinschaft through the grant STR451/7-1 within the SPP1617 priority program. NR 45 TC 0 Z9 0 U1 5 U2 5 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD NOV 18 PY 2016 VL 7 AR 1858 DI 10.3382/fmicb.2016.01858 PG 13 WC Microbiology SC Microbiology GA ED3OD UT WOS:000388757500001 PM 27917168 ER PT J AU Pan, LP Freedman, DS Sharma, AJ Castellanos-Brown, K Park, S Smith, RB Blanck, HM AF Pan, Liping Freedman, David S. Sharma, Andrea J. Castellanos-Brown, Karen Park, Sohyun Smith, Ray B. Blanck, Heidi M. TI Trends in Obesity Among Participants Aged 2-4 Years in the Special Supplemental Nutrition Program for Women, Infants, and Children United States, 2000-2014 SO MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT LA English DT Article ID CHILDHOOD OBESITY C1 [Pan, Liping; Freedman, David S.; Sharma, Andrea J.; Park, Sohyun; Smith, Ray B.; Blanck, Heidi M.] CDC, Div Nutr Phys Act & Obes, Atlanta, GA 30333 USA. [Castellanos-Brown, Karen] Food & Nutr Serv, Special Nutr Res & Anal Div, USDA, Alexandria, VA USA. RP Pan, LP (reprint author), CDC, Div Nutr Phys Act & Obes, Atlanta, GA 30333 USA. EM lpan@cdc.gov NR 10 TC 0 Z9 0 U1 5 U2 5 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 0149-2195 EI 1545-861X J9 MMWR-MORBID MORTAL W JI MMWR-Morb. Mortal. Wkly. Rep. PD NOV 18 PY 2016 VL 65 IS 45 BP 1256 EP 1260 PG 5 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA ED1DX UT WOS:000388585500002 PM 27855143 ER PT J AU Roda, A Nachman, G Weihman, S Cong, MY Zimmerman, F AF Roda, Amy Nachman, Gosta Weihman, Scott Cong, Mary Yong Zimmerman, Fredrick TI Reproductive Ecology of the Giant African Snail in South Florida: Implications for Eradication Programs SO PLOS ONE LA English DT Article ID FULICA GASTROPODA PULMONATA; ACHATINA-FULICA; BEHAVIOR AB Giant African snail (Achatina fulica (Bowdich, 1822)), an important invasive snail, was recently found in South Florida, USA. An extensive eradication effort was initiated consisting of pesticide applications, debris removal and hand collections. We studied the reproduction capacity and population dynamics of snails collected from 22 populations for two years to help evaluate the likely success of the eradication program. A total of 23,890 snails, ranging from 25-131 mm, were measured, dissected and the number of eggs in each snail counted. Gravid snails ranged from 48-128 mm. Only 5% of snails had eggs, which were found year round. As the snails increased in size, they were more likely to include reproducing individuals. However, the percentage of gravid snails peaked when snails were approximately 90 mm. Although more prevalent, small (<65 mm) adults contributed fewer eggs to the population than the larger snails. We evaluated the effect of control measures on six populations having > 1000 adult snails and used data from the two largest populations to investigate how environmental factors (temperature, humidity, and rainfall) interacted with population dynamics and control measures. More snails were collected in weeks with high humidity and more gravid snails were collected when the temperature was higher. The addition of metaldehyde pesticides had the greatest impact on population dynamics by reducing snail numbers. In populations with fewer snails, their numbers were already declining before the use of metaldehyde, although the new treatment accelerated the process. As a consequence of the eradication program, egg-producing snails were no longer collected from most populations by the end of the study. The aggressive and persistent control efforts apparently lead to reduced populations of egg producing snails, eventually resulting in local extinctions of this important pest. C1 [Roda, Amy; Weihman, Scott] US Anim & Plant Hlth Inspect Serv, Miami Lab, Ctr Plant Hlth Sci & Technol, Plant Protect & Quarantine,USDA APHIS, Miami, FL 20737 USA. [Nachman, Gosta] Univ Copenhagen, Sect Ecol & Evolut, Dept Biol, Copenhagen, Denmark. [Cong, Mary Yong] Florida Dept Agr & Consumer Serv, Div Plant Ind, Giant African Land Snail Eradicat Program, Miami, FL USA. [Zimmerman, Fredrick] US Anim & Plant Hlth Inspect Serv, Miami Plant Inspect Stn, Plant Protect & Quarantine, USDA, Miami, FL USA. RP Roda, A (reprint author), US Anim & Plant Hlth Inspect Serv, Miami Lab, Ctr Plant Hlth Sci & Technol, Plant Protect & Quarantine,USDA APHIS, Miami, FL 20737 USA. EM Amy.L.Roda@APHIS.USDA.GOV FU United States Department of Agriculture, Animal Plant Health Inspection Service, Plant Protection and Quarantine FX This work was supported by the United States Department of Agriculture, Animal Plant Health Inspection Service, Plant Protection and Quarantine. NR 34 TC 0 Z9 0 U1 6 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 18 PY 2016 VL 11 IS 11 AR e0165408 DI 10.1371/journal.pone.0165408 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EC7WC UT WOS:000388350300008 PM 27861504 ER PT J AU Sun, ZY Wang, J Ren, H Guo, QF Shu, JW Liu, N AF Sun, Zhongyu Wang, Jun Ren, Hai Guo, Qinfeng Shu, Junwu Liu, Nan TI To What Extent Local Forest Soil Pollen Can Assist Restoration in Subtropical China? SO SCIENTIFIC REPORTS LA English DT Article ID EARLY HOLOCENE OPTIMUM; ECOLOGICAL MEMORY; VEGETATION RELATIONSHIPS; SOUTHERN CHINA; HISTORY; RECORD; CONSERVATION; BIODIVERSITY; PERSPECTIVE; LANDSCAPES AB Long-term ecological data play a vital role in ecological conservation and restoration, however, using information from local forest soil pollen data to assist restoration remains a challenge. This study analyzed two data sets, including 1) surface soil pollen (0-5 cm) and current vegetation data from four near-natural communities and four plantations, and 2) fossil pollen from soil profiles (0-80 cm) from a regional climax community and a degraded land. The pollen representativeness and similarity indexes were calculated. The results showed a low similarity between soil pollen and current vegetation (about 20%) thus forest soil pollen data should be used with caution when defining reference ecosystems. Pollen from Gironniera and Rutaceae which were abundant in broadleaved forest, were also detected in the 40-80 cm layer of a soil profile from the degraded land, which indicates its restoration possibility. Our study considered that the early restoration stage of the study area may benefit from using plant taxa of Pinus, Poaceae, Lonicera, Casuarina, Trema and Quercus. As Pinus, Castanopsis, Gironniera, Rutaceae, Helicia, Randia, Poaceae, Dicranopteris and Pteris always existed during succession, for regional forest restoration under global climate change, the roles of such "stable species" should be considered. C1 [Sun, Zhongyu] Guangzhou Inst Geog, Guangdong Open Lab Geospatial Informat Technol &, Guangzhou 510070, Guangdong, Peoples R China. [Sun, Zhongyu; Wang, Jun; Ren, Hai; Liu, Nan] Chinese Acad Sci, Key Lab Vegetat Restorat & Management Degraded Ec, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China. [Guo, Qinfeng] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, USDA, Res Triangle Pk, NC 27709 USA. [Shu, Junwu] Chinese Acad Sci, Nanjing Inst Geol & Palaeontol, State Key Lab Paleobiol & Stratig, Nanjing 210008, Jiangsu, Peoples R China. RP Wang, J; Ren, H (reprint author), Chinese Acad Sci, Key Lab Vegetat Restorat & Management Degraded Ec, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China. EM wxj@scbg.ac.cn; renhai@scib.ac.cn FU Natural Science Foundation of China [31170493, 31400380]; Guangdong Science and Technology Program [2014A030305014, 2015B070701020] FX This study was supported by the Natural Science Foundation of China (No. 31170493 and 31400380) and Guangdong Science and Technology Program (2014A030305014, 2015B070701020). We thank the staff at Heshan and Dinghushan National Field Research Station of Forest Ecosystem for providing the experimental facilities, and we especially thank Qianmei Zhang, Yongbiao Lin, and Xingquan Rao for their help in the field. We also thank Huilan Zhang for technical help, and Bruce Jaffee for language polishing. NR 44 TC 0 Z9 0 U1 8 U2 8 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD NOV 18 PY 2016 VL 6 AR 37188 DI 10.1038/srep37188 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EC6NO UT WOS:000388253400001 PM 27857187 ER PT J AU Andino, GK Gribskov, M Anderson, DL Evans, JD Hunt, GJ AF Andino, Gladys K. Gribskov, Michael Anderson, Denis L. Evans, Jay D. Hunt, Greg J. TI Differential gene expression in Varroa jacobsoni mites following a host shift to European honey bees (Apis mellifera) SO BMC GENOMICS LA English DT Article DE Apis mellifera; Apis cerana; Asian honey bee; European honey bee; RNA-Seq; Transcriptome; Varroa destructor; Varroa jacobsoni ID COLONY COLLAPSE DISORDER; DEFORMED WING VIRUS; RNA-SEQ DATA; REFERENCE GENOME; ANNOTATION; DESTRUCTOR; LOSSES; QUANTIFICATION; POPULATIONS; ALIGNMENT AB Background: Varroa mites are widely considered the biggest honey bee health problem worldwide. Until recently, Varroa jacobsoni has been found to live and reproduce only in Asian honey bee (Apis cerana) colonies, while V. destructor successfully reproduces in both A. cerana and A. mellifera colonies. However, we have identified an island population of V. jacobsoni that is highly destructive to A. mellifera, the primary species used for pollination and honey production. The ability of these populations of mites to cross the host species boundary potentially represents an enormous threat to apiculture, and is presumably due to genetic variation that exists among populations of V. jacobsoni that influences gene expression and reproductive status. In this work, we investigate differences in gene expression between populations of V. jacobsoni reproducing on A. cerana and those either reproducing or not capable of reproducing on A. mellifera, in order to gain insight into differences that allow V. jacobsoni to overcome its normal species tropism. Results: We sequenced and assembled a de novo transcriptome of V. jacobsoni. We also performed a differential gene expression analysis contrasting biological replicates of V. jacobsoni populations that differ in their ability to reproduce on A. mellifera. Using the edgeR, EBSeq and DESeq R packages for differential gene expression analysis, we found 287 differentially expressed genes (FDR <= 0.05), of which 91% were up regulated in mites reproducing on A. mellifera. In addition, mites found reproducing on A. mellifera showed substantially more variation in expression among replicates. We searched for orthologous genes in public databases and were able to associate 100 of these 287 differentially expressed genes with a functional description. Conclusions: There is differential gene expression between the two mite groups, with more variation in gene expression among mites that were able to reproduce on A. mellifera. A small set of genes showed reduced expression in mites on the A. mellifera host, including putative transcription factors and digestive tract developmental genes. The vast majority of differentially expressed genes were up-regulated in this host. This gene set showed enrichment for genes associated with mitochondrial respiratory function and apoptosis, suggesting that mites on this host may be experiencing higher stress, and may be less optimally adapted to parasitize it. Some genes involved in reproduction and oogenesis were also overexpressed, which should be further studied in regards to this host shift. C1 [Andino, Gladys K.] Purdue Univ, Res Comp, Informat Technol Purdue, W Lafayette, IN 47907 USA. [Gribskov, Michael] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. [Anderson, Denis L.] Kuwaitat Res Stn, Abu Dhabi Food Control Author, Al Ain, U Arab Emirates. [Evans, Jay D.] USDA, Beltsville Agr Res Ctr East, Bee Res Lab, Beltsville, MD 20705 USA. [Hunt, Greg J.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. RP Andino, GK (reprint author), Purdue Univ, Res Comp, Informat Technol Purdue, W Lafayette, IN 47907 USA. EM gandino@purdue.edu FU USDA-ARS; USDA National Institute of Food and Agriculture [2009-05254] FX The financial support for this study and an ongoing full genome sequencing project was provided by USDA-ARS and the USDA National Institute of Food and Agriculture (grant 2009-05254). We thank Catherine Hill and Jeff Stuart for helpful manuscript comments. We thank Rick Westerman and Phillip San Miguel for their advice in regards to libraries preparation and preliminary analysis of the sequence data. We also thank Ann Christine Catlin for helping to create the database, were the raw reads data and assemblies of this mite has been deposited. NR 47 TC 0 Z9 0 U1 34 U2 34 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD NOV 16 PY 2016 VL 17 AR 926 DI 10.1186/s12864-016-3130-3 PG 12 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA EC4WA UT WOS:000388133900004 PM 27852222 ER PT J AU Jaisi, DP Li, H Wallace, AF Paudel, P Sun, MJ Balakrishna, A Lerch, RN AF Jaisi, Deb P. Li, Hui Wallace, Adam F. Paudel, Prajwal Sun, Mingjing Balakrishna, Avula Lerch, Robert N. TI Mechanisms of Bond Cleavage during Manganese Oxide and UV Degradation of Glyphosate: Results from Phosphate Oxygen Isotopes and Molecular Simulations SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE glyphosate; degradation; manganese oxide; phosphate isotopes; DFT; C-P and C-N bond cleavage ID GAUSSIAN-TYPE BASIS; CATALYZED DEGRADATION; ORGANIC-MOLECULES; AQUEOUS-SOLUTION; PHOSPHORUS BOND; TRACING SOURCES; ORBITAL METHODS; SURFACE-WATER; ACID; OXIDATION AB Degradation of glyphosate in the presence of manganese oxide and UV light was analyzed using phosphate oxygen isotope ratios and density function theory (DFT). The preference of C-P or C-N bond cleavage was found to vary with changing glyphosate/manganese oxide ratios, indicating the potential role of sorption-induced conformational changes on the composition of intermediate degradation products. Isotope data confirmed that one oxygen atom derived solely from water was incorporated into the released phosphate during glyphosate degradation, and this might suggest similar nucleophilic substitution at P centers and C-P bond cleavage both in manganese oxide-and UV light-mediated degradation. The DFT results reveal that the C-P bond could be cleaved by water, OH- or (OH)-O-center dot, with the energy barrier opposing bond dissociation being lowest in the presence of the radical species, and that C-N bond cleavage is favored by the formation of both nitrogen-and carbon-centered radicals. Overall, these results highlight the factors controlling the dominance of C-P or C-N bond cleavage that determines the composition of intermediate/final products and ultimately the degradation pathway. C1 [Jaisi, Deb P.; Li, Hui; Paudel, Prajwal; Sun, Mingjing; Balakrishna, Avula] Univ Delaware, Dept Plant & Soil Sci, Newark, DE 19716 USA. [Wallace, Adam F.] Univ Delaware, Dept Geol Sci, Newark, DE 19716 USA. [Lerch, Robert N.] USDA ARS, Cropping Syst & Water Qual Res Unit, Columbia, MO 65211 USA. RP Jaisi, DP (reprint author), Univ Delaware, Dept Plant & Soil Sci, Newark, DE 19716 USA. EM Jaisi@udel.edu FU University of Delaware; Delaware EPSCoR [1301765]; Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2013-67019-21373] FX This research was supported by the start-up fund at the University of Delaware to D.P.J. It was supported partly by Delaware EPSCoR (1301765) and Agriculture and Food Research Initiative Competitive Grant 2013-67019-21373 from the USDA National Institute of Food and Agriculture. NR 56 TC 1 Z9 1 U1 15 U2 15 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD NOV 16 PY 2016 VL 64 IS 45 BP 8474 EP 8482 DI 10.1021/acs.jafc.6b02608 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA EC8ZF UT WOS:000388430900002 PM 27775891 ER PT J AU Song, B Oehrle, NW Liu, SS Krishnan, HB AF Song, Bo Oehrle, Nathan W. Liu, Shanshan Krishnan, Hari B. TI Characterization of Seed Storage Proteins of Several Perennial Glycine Species SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE soybean; perennial Glycine species; glycinin; beta-conglycinin; Kunitz trypsin inhibitor; Bowman-Birk inhibitor ID MAX L MERR; KUNITZ TRYPSIN-INHIBITOR; QUANTITATIVE TRAIT LOCUS; TOMENTELLA HAYATA; SOYBEAN CULTIVARS; GENOMIC RELATIONSHIPS; INTERSUBGENERIC HYBRIDS; INTERSPECIFIC HYBRIDS; WIDE HYBRIDIZATION; EMBRYO CULTURE AB Perennial Glycine species, distant relatives of soybean, have been recognized as a potential source of new genetic diversity for soybean improvement. The subgenus Glycine includes around 30 perennial species, which are well-adapted to drought conditions and possess resistance to a number of soybean pathogens. In spite of the potential of the perennial Glycine species for soybean improvement, very little is known about their storage proteins and their relationship with cultivated soybean seed proteins. We have examined the seed protein composition of nine perennial Glycine species by one-and two-dimensional (1-D and 2-D) gel electrophoresis. The relationship between cultivated soybean and perennial soybean seed proteins was examined by immunoblot analyses using antibodies raised against G. max beta-conglycinin, glycinin A3 subunit, lipoxygenase, leginsulin, Kunitz trypsin inhibitor, and Bowman-Birk protease inhibitor. Additionally, we have measured the trypsin and chymotrypsin inhibitor activities from cultivated soybean and perennial Glycine species and have found marked differences between them. Our 2-D gel and immunoblot analyses demonstrate significant differences in the protein composition and size heterogeneities of the 7S and 11S seed storage proteins of soybean and perennial Glycine species. Perennial Glycine species accumulated a 45 kDa protein that was not detected in G. max and G. sofa. This unique 45 kDa protein was immunologically related to the A3 glycinin subunit of G. max. The results of our studies suggest that even though the seed proteins of wild perennial Glycine species and G. max are immunologically related, their genes have diverged from each other during the course of evolution. C1 [Song, Bo; Oehrle, Nathan W.; Krishnan, Hari B.] USDA ARS, Plant Genet Res Unit, Columbia, MO 65211 USA. [Song, Bo; Liu, Shanshan] Northeast Agr Univ, Key Lab Soybean Biol, Chinese Minist Educ, Harbin, Peoples R China. [Krishnan, Hari B.] Univ Missouri, Plant Sci Div, Columbia, MO 65211 USA. RP Krishnan, HB (reprint author), Univ Missouri, USDA ARS, Plant Genet Res Unit, 108 Curtis Hall, Columbia, MO 65211 USA. EM Hari.Krishnan@ars.usda.gov FU USDA-Agricultural Research Service FX This research was supported by the USDA-Agricultural Research Service. NR 48 TC 0 Z9 0 U1 3 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD NOV 16 PY 2016 VL 64 IS 45 BP 8499 EP 8508 DI 10.1021/acs.jafc.6b03677 PG 10 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA EC8ZF UT WOS:000388430900005 PM 27794605 ER PT J AU Wiesinger, JA Cichy, KA Glahn, RP Grusak, MA Brick, MA Thompson, HJ Tako, E AF Wiesinger, Jason A. Cichy, Karen A. Glahn, Raymond P. Grusak, Michael A. Brick, Mark A. Thompson, Henry J. Tako, Elad TI Demonstrating a Nutritional Advantage to the Fast-Cooking Dry Bean (Phaseolus vulgaris L.) SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE dry beans; Phaseolus vulgaris L.; cooking time; protein; iron; zinc; phytate; Caco2 cell culture; iron bioavailability ID VITRO DIGESTION/CACO-2 MODEL; POULTRY GALLUS-GALLUS; CELL-WALL STRUCTURE; LOW IRON STATUS; PHYTIC ACID; COMMON BEANS; BIOAVAILABLE IRON; DIETARY FIBER; BIOFORTIFIED BEANS; GERMPLASM LINES AB Dry beans (Phaseolus vulgaris L.) are a nutrient-dense food rich in protein and micronutrients. Despite their nutritional benefits, long cooking times limit the consumption of dry beans worldwide, especially in nations where fuelwood for cooking is often expensive or scarce. This study evaluated the nutritive value of 12 dry edible bean lines that vary for cooking time (20-89 min) from four market classes (yellow, cranberry, light red kidney, and red mottled) of economic importance in bean-consuming regions of Africa and the Americas. When compared to their slower cooking counterparts within each market class, fast-cooking dry beans retain more protein and minerals while maintaining similar starch and fiber densities when fully cooked. For example, some of the highest protein and mineral retention values were measured in the fast-cooking yellow bean cultivar Cebo Cela, which offered 20% more protein, 10% more iron, and 10% more zinc with each serving when compared with Canario, a slow-cooking yellow bean that requires twice the cooking time to become palatable. A Caco-2 cell culture model also revealed the bioavailability of iron is significantly higher in faster cooking entries (r = -0.537, P = 0.009) as compared to slower cooking entries in the same market class. These findings suggest that fast-cooking bean varieties have improved nutritive value through greater nutrient retention and improved iron bioavailability. C1 [Wiesinger, Jason A.; Cichy, Karen A.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Cichy, Karen A.] Michigan State Univ, USDA ARS, Sugarbeet & Bean Res Unit, E Lansing, MI 48824 USA. [Glahn, Raymond P.; Tako, Elad] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Grusak, Michael A.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Brick, Mark A.] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Thompson, Henry J.] Colorado State Univ, Dept Hort & Landscape Architecture, Ft Collins, CO 80523 USA. RP Cichy, KA (reprint author), Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA.; Cichy, KA (reprint author), Michigan State Univ, USDA ARS, Sugarbeet & Bean Res Unit, E Lansing, MI 48824 USA. EM karen.cichy@ars.usda.gov FU Norman Borlaug Commemorative Research Initiative of the U.S. Agency for International Development [021-22310-004-11R]; U.S. Department of Agriculture, Agricultural Research Service Projects [5050-21430-01000D, 8062-52000-001-00-D, 58-6250-0-008]; National Cancer Institute [R01-CA172375] FX This work was supported in part by funding from the Norman Borlaug Commemorative Research Initiative of the U.S. Agency for International Development, Project 021-22310-004-11R, and by the U.S. Department of Agriculture, Agricultural Research Service Projects 5050-21430-01000D (K.A.C.), 8062-52000-001-00-D (RP.G.), and 58-6250-0-008 (M.A.G.). This work was also supported in part by PHS Grant R01-CA172375 from the National Cancer Institute (H.J.T.). The contents of this publication do not necessarily reflect the views or policies of the U.S. Department of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. government. NR 85 TC 0 Z9 0 U1 14 U2 14 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD NOV 16 PY 2016 VL 64 IS 45 BP 8592 EP 8603 DI 10.1021/acs.jafc.6b03100 PG 12 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA EC8ZF UT WOS:000388430900016 PM 27754657 ER PT J AU Hertach, T Puissant, S Gogala, M Trilar, T Hagmann, R Baur, H Kunz, G Wade, EJ Loader, SP Simon, C Nagel, P AF Hertach, Thomas Puissant, Stephane Gogala, Matija Trilar, Tomi Hagmann, Reto Baur, Hannes Kunz, Gernot Wade, Elizabeth J. Loader, Simon P. Simon, Chris Nagel, Peter TI Complex within a Complex: Integrative Taxonomy Reveals Hidden Diversity in Cicadetta brevipennis (Hemiptera: Cicadidae) and Unexpected Relationships with a Song Divergent Relative SO PLOS ONE LA English DT Article ID NEW-ZEALAND CICADAS; SPECIES DELIMITATION; MITOCHONDRIAL-DNA; GENE-FLOW; PHYLOGENETIC UTILITY; MONTANA COMPLEX; CALLING SONGS; SENSU-LATO; SPECIATION; PHYLOGEOGRAPHY AB Multiple sources of data in combination are essential for species delimitation and classification of difficult taxonomic groups. Here we investigate a cicada taxon with unusual cryptic diversity and we attempt to resolve seemingly contradictory data sets. Cicada songs act as species-specific premating barriers and have been used extensively to reveal hidden taxonomic diversity in morphologically similar species. The Palaearctic Cicadetta montana species complex is an excellent example where distinct song patterns have disclosed multiple recently described species. Indeed, two taxa turned out to be especially diverse in that they form a "complex within the complex" : the Cicadetta cerdaniensis song group (four species studied previously) and Cicadetta brevipennis (examined in details here). Based on acoustic, morphological, molecular, ecological and spatial data sampled throughout their broad European distribution, we find that Cicadetta brevipennis s.l. comprises five lineages. The most distinct lineage is identified as Cicadetta petryi Schumacher, 1924, which we reassign to the species level. Cicadetta brevipennis litoralis Puissant & Hertach ssp. n. and Cicadetta brevipennis hippolaidica Hertach ssp. n. are new to science. The latter hybridizes with Cicadetta brevipennis brevipennis Fieber, 1876 at a zone inferred from intermediate song patterns. The fifth lineage requires additional investigation. The C. cerdaniensis and the C. brevipennis song groups exhibit characteristic, clearly distinct basic song patterns that act as reproductive barriers. However, they remain completely intermixed in the Bayesian and maximum likelihood COI and COII mitochondrial DNA phylogenies. The closest relative of each of the four cerdaniensis group species is a brevipennis group taxon. In our favoured scenario the phylogenetic pairs originated in common Pleistocene glacial refuges where the taxa speciated and experienced sporadic inter-group hybridization leading to extensive introgression and mitochondrial capture. C1 [Hertach, Thomas; Hagmann, Reto; Loader, Simon P.; Nagel, Peter] Univ Basel, Dept Environm Sci, Biogeog, Basel, Switzerland. [Hertach, Thomas; Hagmann, Reto; Baur, Hannes] Burgergemeinde Bern, Nat Hist Museum, Dept Invertebrates, Bern, Switzerland. [Puissant, Stephane] Museum Jardin Sci, Dijon, France. [Puissant, Stephane] Sorbonne Univ, Museum Natl Hist Nat, Inst Systemat, Evolut,Biodiversite, Paris, France. [Gogala, Matija] Slovenian Acad Sci & Arts, Ljubljana, Slovenia. [Trilar, Tomi] Slovenian Museum Nat Hist, Ljubljana, Slovenia. [Baur, Hannes] Univ Bern, Inst Ecol & Evolut, Bern, Switzerland. [Kunz, Gernot] Karl Franzens Univ Graz, Dept Zool, Graz, Austria. [Wade, Elizabeth J.; Simon, Chris] Univ Connecticut, Dept Ecol & Evolutionary Biol, Storrs, CT USA. [Wade, Elizabeth J.] USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. [Loader, Simon P.] Univ Roehampton, Dept Life Sci, London, England. RP Hertach, T (reprint author), Univ Basel, Dept Environm Sci, Biogeog, Basel, Switzerland.; Hertach, T (reprint author), Burgergemeinde Bern, Nat Hist Museum, Dept Invertebrates, Bern, Switzerland. EM thomas.hertach@unibas.ch FU Wolfermann-Nageli Foundation; Zigerli-Hegi Foundation; Walter Haefner Foundation; Paul Schiller Foundation; Biedermann-Mantel Foundation; Temperatio Foundation; Otto Gamma Foundation and Pro Natura; Ministry of Higher Education, Science and Technology of the Republic of Slovenia [P1-0255]; US National Science Foundation [DEB-0955849] FX The Swiss cicada project (TH) was financed by the following funders: Wolfermann-Nageli Foundation; Zigerli-Hegi Foundation; Walter Haefner Foundation; Paul Schiller Foundation (http://www.paul-schiller-stiftung.ch); Biedermann-Mantel Foundation; Temperatio Foundation (http://www.temperatio.ch); Otto Gamma Foundation and Pro Natura (http://www.pronatura.ch). The travel expenses in Italy (TH) are covered by the Freiwillige Akademische Gesellschaft Basel (http://www.fag-basel.ch). The research of TT was part of the programme "Communities, relations and communications in the ecosystems" (no. P1-0255), financed by the Ministry of Higher Education, Science and Technology of the Republic of Slovenia. The work of EW and CS was supported by the US National Science Foundation (http:/www.nsf.gov) under grant number DEB-0955849. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 109 TC 1 Z9 1 U1 2 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 16 PY 2016 VL 11 IS 11 AR e0165562 DI 10.1371/journal.pone.0165562 PG 41 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EC1ZU UT WOS:000387909300020 PM 27851754 ER PT J AU Feng, WY Zhu, YR Wu, FC He, ZQ Zhang, C Giesy, JP AF Feng, Weiying Zhu, Yuanrong Wu, Fengchang He, Zhongqi Zhang, Chen Giesy, John P. TI Forms and Lability of Phosphorus in Algae and Aquatic Macrophytes Characterized by Solution P-31 NMR Coupled with Enzymatic Hydrolysis SO SCIENTIFIC REPORTS LA English DT Article ID MAGNETIC-RESONANCE-SPECTROSCOPY; SOIL ORGANIC PHOSPHORUS; NAOH-EDTA EXTRACTS; LAKE-SEDIMENTS; CHINA; WATER; FRACTIONS; BIOAVAILABILITY; PRESERVATION; ASSIGNMENTS AB Solution Phosphorus-31 nuclear magnetic resonance (P-31 NMR) spectroscopy coupled with enzymatic hydrolysis (EH) with commercially available phosphatases was used to characterize phosphorus (P) compounds in extracts of the dominant aquatic macrophytes and algae in a eutrophic lake. Total extractable organic P (P-o) concentrations ranged from 504 to 1643 mg kg(-1) and 2318 to 8395 mg kg-1 for aquatic macrophytes and algae, respectively. Using 31P NMR spectroscopy, 11 P-o species were detected in the mono-and diester region. Additionally, orthophosphate, pyrophosphate and phosphonates were also detected. Using EH, phytate-like P was identified as the prevalent class of enzyme-labile Po, followed by labile monoester-and diester-P. Comparison of the NMR and EH data indicated that the distribution pattern of major P forms in the samples determined by the two methods was similar (r = 0.712, p < 0.05). Additional P-31 NMR spectroscopic analysis of extracts following EH showed significant decreases in the monoester and pyrophosphate regions, with a corresponding increase in the orthophosphate signal, as compared to unhydrolyzed extracts. Based on these quantity and hydrolysis data, we proposed that recycling of P-o in vegetative biomass residues is an important mechanism for long-term self-regulation of available P for algal blooming in eutrophic lakes. C1 [Feng, Weiying; Zhu, Yuanrong; Wu, Fengchang; Zhang, Chen; Giesy, John P.] Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing 100012, Peoples R China. [Feng, Weiying] Beijing Normal Univ, Coll Water Sci, Beijing 100875, Peoples R China. [He, Zhongqi] USDA ARS, Southern Reg Res Ctr, New Orleans, LA 70124 USA. [Giesy, John P.] Univ Saskatchewan, Dept Biomed Vet Sci, Saskatoon, SK S7N 5B3, Canada. [Giesy, John P.] Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK S7N 5B3, Canada. RP Zhu, YR (reprint author), Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing 100012, Peoples R China. EM zhuyuanrong07@mails.ucas.ac.cn FU National Natural Science Foundation of China [41630645, 41403094, 41403743] FX The present is study was supported in part by the National Natural Science Foundation of China (41630645, 41403094, 41403743). Special thanks go to an anonymous reviewer for his/her detailed editing of the manuscript which greatly improved the readability of this work. NR 43 TC 0 Z9 0 U1 12 U2 12 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD NOV 16 PY 2016 VL 6 AR 37164 DI 10.1038/srep37164 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EC1PA UT WOS:000387876800001 PM 27849040 ER PT J AU Nam, S Condon, BD Delhom, CD Fontenot, KR AF Nam, Sunghyun Condon, Brian D. Delhom, Christopher D. Fontenot, Krystal R. TI Silver-cotton nanocomposites: Nano-design of microfibrillar structure causes morphological changes and increased tenacity SO SCIENTIFIC REPORTS LA English DT Article ID POLYMER NANOCOMPOSITES; FIBERS; CELLULOSE; DYNAMICS; MERCERIZATION; NANOPARTICLES; COMPOSITES; STRENGTH AB The interactions of nanoparticles with polymer hosts have important implications for directing the macroscopic properties of composite fibers, yet little is known about such interactions with hierarchically ordered natural polymers due to the difficulty of achieving uniform dispersion of nanoparticles within semi-crystalline natural fiber. In this study we have homogeneously dispersed silver nanoparticles throughout an entire volume of cotton fiber. The resulting electrostatic interaction and distinct supramolecular structure of the cotton fiber provided a favorable environment for the controlled formation of nanoparticles (12 +/- 3 nm in diameter). With a high surface-to-volume ratio, the extensive interfacial contacts of the nanoparticles efficiently "glued" the structural elements of microfibrils together, producing a unique inorganic-organic hybrid substructure that reinforced the multilayered architecture of the cotton fiber. C1 [Nam, Sunghyun; Condon, Brian D.; Delhom, Christopher D.; Fontenot, Krystal R.] ARS, Southern Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. RP Nam, S (reprint author), ARS, Southern Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM sunghyun.nam@ars.usda.gov NR 32 TC 0 Z9 0 U1 6 U2 6 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD NOV 16 PY 2016 VL 6 AR 37320 DI 10.1038/srep37320 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EC4EX UT WOS:000388080200001 PM 27849038 ER PT J AU Wang, Y Xu, L Gu, YQ Coleman-Derr, D AF Wang, Yi Xu, Ling Gu, Yong Q. Coleman-Derr, Devin TI MetaCoMET: a web platform for discovery and visualization of the core microbiome SO BIOINFORMATICS LA English DT Article ID VENN-DIAGRAM AB Motivation: A key component of the analysis of microbiome datasets is the identification of OTUs shared between multiple experimental conditions, commonly referred to as the core microbiome. Results: We present a web platform named MetaCoMET that enables the discovery and visualization of the core microbiome and provides a comparison of the relative abundance and diversity patterns between subsets of samples within a microbiome dataset. MetaCoMET provides an efficient and interactive graphical interface for analyzing each subset defined by the union or disjunction of groups within the Venn diagram, and includes a graphical taxonomy summary, alpha diversity metrics, Principal Coordinate analysis, abundance-based heatmaps, and a chart indicating the geographic distribution of each sample. C1 [Wang, Yi; Gu, Yong Q.] USDA ARS, Western Reg Res Ctr, Crop Improvement & Genet Res Unit, 800 Buchanan St, Albany, CA 94710 USA. [Wang, Yi; Xu, Ling; Coleman-Derr, Devin] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Wang, Yi; Xu, Ling; Coleman-Derr, Devin] USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA. RP Coleman-Derr, D (reprint author), Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA.; Coleman-Derr, D (reprint author), USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA. EM devin.coleman-derr@ars.usda.gov FU United States Department of Agriculture [2030-21430-008-00D] FX This work has been supported by the United States Department of Agriculture [2030-21430-008-00D]. NR 11 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1367-4803 EI 1460-2059 J9 BIOINFORMATICS JI Bioinformatics PD NOV 15 PY 2016 VL 32 IS 22 BP 3469 EP 3470 DI 10.1093/bioinformatics/btw507 PG 2 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Computer Science, Interdisciplinary Applications; Mathematical & Computational Biology; Statistics & Probability SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Computer Science; Mathematical & Computational Biology; Mathematics GA EI8IE UT WOS:000392748700013 PM 27485442 ER PT J AU McGill, JL Sacco, RE AF McGill, Jodi L. Sacco, Randy E. TI gamma delta T cells and the immune response to respiratory syncytial virus infection SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article; Proceedings Paper CT Meeting on Immunity to Veterinary Pathogens - Informing Vaccine Development CY 2015 CL Keystone, CO DE Bovine respiratory syncytial virus; Bovine respiratory disease; Human respiratory syncytial virus; Gamma delta T cells; Immune response ID LYMPHOCYTE SUBSETS; DIFFERENTIAL CHEMOKINE; DISEASE; EXPRESSION; CALVES; CHILDREN; INFANTS; IMMUNOPATHOLOGY; CHALLENGE; RECEPTORS AB gamma delta T cells are a subset of nonconventional T cells that play a critical role in bridging the innate and adaptive arms of the immune system. gamma delta T cells are particularly abundant in ruminant species and may constitute up to 60% of the circulating lymphocyte pool in young cattle. The frequency of circulating gamma delta T cells is highest in neonatal calves and declines as the animal ages, suggesting these cells may be particularly important in the immune system of the very young. Bovine respiratory syncytial virus (BRSV) is a significant cause of respiratory infection in calves, and is most severe in animals under one year of age. BRSV is also a significant factor in the development of bovine respiratory disease complex (BRDC), the leading cause of morbidity and mortality in feedlot cattle. Human respiratory syncytial virus (RSV) is closely related to BRSV and a leading cause of lower respiratory tract infection in infants and children worldwide. BRSV infection in calves shares striking similarities with RSV infection in human infants. To date, there have been few studies defining the role of gamma delta T cells in the immune response to BRSV or RSV infection in animals or humans, respectively. However, emerging evidence suggests that gamma delta T cells may play a critical role in the early recognition of infection and in shaping the development of the adaptive immune response through inflammatory chemokine and cytokine production. Further, while it is clear that gamma delta T cells accumulate in the lungs during BRSV and RSV infection, their role in protection vs. immunopathology remains unclear. This review will summarize what is currently known about the role of gamma delta T cells in the immune response to BRSV and BRDC in cattle, and where appropriate, draw parallels to the role of gamma delta T cells in the human response to RSV infection. (C) 2016 Elsevier B.V. All rights reserved. C1 [McGill, Jodi L.] Kansas State Univ, Dept Diagnost Med & Pathobiol, 1800 Denison Ave, Manhattan, KS 66503 USA. [Sacco, Randy E.] ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, USDA, 1920 Dayton Ave, Ames, IA 50010 USA. RP McGill, JL (reprint author), Kansas State Univ, Dept Diagnost Med & Pathobiol, 1800 Denison Ave, Manhattan, KS 66503 USA. EM jlmcgill@vet.k-state.edu NR 57 TC 0 Z9 0 U1 2 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 EI 1873-2534 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD NOV 15 PY 2016 VL 181 BP 24 EP 29 DI 10.1016/j.vetimm.2016.02.012 PG 6 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA EF7MB UT WOS:000390512600005 PM 26923879 ER PT J AU Pedersen, LE Patch, JR Kenney, M Glabman, RA Nielsen, M Jungersen, G Buus, S Golde, WT AF Pedersen, Lasse E. Patch, Jared R. Kenney, Mary Glabman, Raisa A. Nielsen, Morten Jungersen, Gregers Buus, Soren Golde, William T. TI Expanding specificity of class I restricted CD8(+) T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (FMDV) vaccine SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article; Proceedings Paper CT Meeting on Immunity to Veterinary Pathogens - Informing Vaccine Development CY 2015 CL Keystone, CO DE Cytotoxic T lymphocytes; MHC; SLA; Class I; MHC tetramers; T cell epitopes ID BINDING SPECIFICITIES; GERMINAL-CENTERS; PEPTIDE-BINDING; PREDICTION; MOLECULES; ANTIGEN; IDENTIFICATION; PROTEINS; DYNAMICS AB The immune response to the highly acute foot-and-mouth disease virus (FMDV) is routinely reported as a measure of serum antibody. However, a critical effector function of immune responses combating viral infection of mammals is the cytotoxic T lymphocyte (CTL) response mediated by virus specific CD8 expressing T cells. This immune mechanism arrests viral spread by killing virus infected cells before new, mature virus can develop. We have previously shown that infection of swine by FMDV results in a measurable CTL response and have correlated CTL killing of virus-infected cells with specific class I major histocompatibility complex (MHC) tetramer staining. We also showed that a modified replication defective human adenovirus 5 vector expressing the FMDV structural proteins (Ad5-FMDV-T vaccine) targets the induction of a CD8(+) CTL response with a minimal humoral response. In this report, we show that the specificity of the CD8(+) T cell response to Ad5-FMDV-T varies between cohorts of genetically identical animals. Further, we demonstrate epitope specificity of CD8(+) T cells expands following multiple immunizations with this vaccine. Published by Elsevier B.V. C1 [Pedersen, Lasse E.; Patch, Jared R.; Kenney, Mary; Glabman, Raisa A.; Golde, William T.] ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY 11944 USA. [Pedersen, Lasse E.; Nielsen, Morten; Jungersen, Gregers] Tech Univ Denmark, Natl Vet Inst, Lyngby, Denmark. [Pedersen, Lasse E.; Nielsen, Morten; Jungersen, Gregers] Tech Univ Denmark, Ctr Biol Sequence Anal, Lyngby, Denmark. [Patch, Jared R.; Glabman, Raisa A.] Univ Vermont, Dept Anim & Vet Sci, Burlington, VT USA. [Nielsen, Morten] Univ Nacl San Martin, Inst Invest Biotecnol, Buenos Aires, DF, Argentina. [Buus, Soren] Univ Copenhagen, Copenhagen, Denmark. RP Golde, WT (reprint author), ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY 11944 USA. EM wtgolde@aol.com NR 23 TC 0 Z9 0 U1 2 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 EI 1873-2534 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD NOV 15 PY 2016 VL 181 BP 62 EP 70 DI 10.1016/j.vetimm.2016.07.012 PG 9 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA EF7MB UT WOS:000390512600009 PM 27498407 ER PT J AU Ding, J Bai, X Wang, XL Wang, YF Shi, HN Rosenthal, B Boireau, P Wu, XP Liu, MY Liu, XL AF Ding, J. Bai, X. Wang, X. L. Wang, Y. F. Shi, H. N. Rosenthal, B. Boireau, P. Wu, X. P. Liu, M. Y. Liu, X. L. TI Developmental profile of select immune cells in mice infected with Trichinella spiralis during the intestinal phase SO VETERINARY PARASITOLOGY LA English DT Article; Proceedings Paper CT 14th International Conference on Trichinellosis (ICT) CY SEP 14-18, 2015 CL Berlin, GERMANY SP Fed Inst Risk Assessment, Free Univ Berlin, Fac Vet Med, German Soc Vet Med, Sect Parasitol, Fed Minist Food & Agr DE Trichinella spiralis; Immune response; T cells; B cells; CD41+/CD8+ratio; Macrophages ID IN-VITRO; WORM EXPULSION; T-CELLS; APOPTOSIS; MACROPHAGES; SUPPRESSION; MECHANISM; STAGE AB Trichinella spiralis can cause immunosuppression during the intestinal phase of early infection. However, changes in the peripheral blood during T. spiralis early infection remain unclear. Here, select immune cells in mice infected with 500 muscle larvae (ML) of T. spiralis during the intestinal phase of infection were studied. First, the recovery rates of the intestinal worms and female fecundity were determined, and the results showed that the intestinal worms were completely eliminated at 17 days post-infection (dpi) and that large numbers of new-born larvae (NBL) were generated from 5 to 9 dpi. Using flow cytometry, it was shown that the number of CD4+ T cells and CD8+ T cells increased over the entire intestinal phase, except on 7 dpi when CD4+ T cells decreased significantly compared to the control groups. Although both CD4+ and CD8+ T cells increased, CD8+ T cells increased more than CD4+ T cells, leading to a lower CD4+/CD8+ ratio compared to the control group. Subsequently, a depression of the proliferative response of T cells to concanavalin A (Con A) was noticed at 7 and 11 dpi. Although the proliferative response of B cells to LPS was enhanced, the number of B cells from mouse peripheral blood stimulated by T. spiralis antigens showed no differences with the control group prior to 11 dpi. The expression of CD14 on monocytemacrophages decreased during the same period, which meant that the antigen-presenting response was reduced in the immune system of the infected mice. Moreover, the alternatively activated macrophages were induced in T. spiralis early infection. These data provide a better understanding of the development of the intestinal immune response in mice infected with T. spiralis. (C) 2016 Elsevier B.V. All rights reserved. C1 [Ding, J.; Bai, X.; Wang, X. L.; Wang, Y. F.; Wu, X. P.; Liu, M. Y.; Liu, X. L.] Jilin Univ, Natl Inst Parasit Dis, Chinese Ctr Dis Control & Prevent, Shanghai Key Lab Zoonoses Res,Minist Educ,Inst Zo, Changchun, Peoples R China. [Shi, H. N.] Massachusetts Gen Hosp East, Mucosal Immunol Lab, Pediat Gastroenterol Unit, Boston, MA USA. [Rosenthal, B.] USDA, Anim Parasit Dis Lab, Bldg 1180, Beltsville, MD 20705 USA. [Boireau, P.] Hlth Anim Lab, ANSES, Maisons Alfort, France. [Liu, M. Y.] Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China. RP Wu, XP; Liu, MY; Liu, XL (reprint author), Jilin Univ, Natl Inst Parasit Dis, Chinese Ctr Dis Control & Prevent, Shanghai Key Lab Zoonoses Res,Minist Educ,Inst Zo, Changchun, Peoples R China. EM lvy.2001@163.com; liumy@jlu.edu.cn; liuxlei@163.com NR 30 TC 0 Z9 0 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD NOV 15 PY 2016 VL 231 SI SI BP 77 EP 82 DI 10.1016/j.vetpar.2016.07.019 PG 6 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA EG0LK UT WOS:000390724100013 PM 27501987 ER PT J AU Bai, X Wang, XL Tang, B Shi, HN Boireau, P Rosenthal, B Wu, XP Liu, MY Liu, XL AF Bai, X. Wang, X. L. Tang, B. Shi, H. N. Boireau, P. Rosenthal, B. Wu, X. P. Liu, M. Y. Liu, X. L. TI The roles of supernatant of macrophage treated by excretory-secretory products from muscle larvae of Trichinella spiralis on the differentiation of C2C12 myoblasts SO VETERINARY PARASITOLOGY LA English DT Article; Proceedings Paper CT 14th International Conference on Trichinellosis (ICT) CY SEP 14-18, 2015 CL Berlin, GERMANY SP Fed Inst Risk Assessment, Free Univ Berlin, Fac Vet Med, German Soc Vet Med, Sect Parasitol, Fed Minist Food & Agr DE Trichinella spiralis; Excretory-secretory products; J774A.1 macrophage; C2C12 myoblast ID NURSE CELL-FORMATION; SKELETAL-MUSCLE; IN-VITRO; MYOGENIC DIFFERENTIATION; SATELLITE CELLS; EXPRESSION; PROTEIN; REGENERATION; PROLIFERATION; TRICHINOSIS AB The excretory-secretory products (ESPs) released by the muscle-larvae (ML) stage of Trichinella spiralis have been suggested to be involved in nurse cell formation. However, the molecular mechanisms by which ML-ESPs modulate nurse cell formation remain unclear. Macrophages exert either beneficial or deleterious effects on tissue repair, depending on their activation/polarization state. They are crucial for skeletal muscle repair, notably, via their actions on myogenic precursor cells. However, these interactions during T. spiralis infection have not been characterized. In the present study, the ability of conditioned medium (CM) from J774A.1 macrophages treated with ML-ESPs to influence the differentiation of murine myoblasts, and the mechanisms of this influence, were investigated in vitro. The results showed that the expression of Myogenic Regulatory Factors (MRFs) MyoD and myogenin, myosin heavy chain (MyHC), and the p21 cyclin-dependent kinase inhibitor were reduced in CM treated cells compared to their expression in the control group. These findings indicated that CM inhibited myoblast differentiation. Conversely, CM promoted myoblast proliferation and increased cyclin D1 levels. Taken together, results of our study suggested that CM can indirectly influence myoblast differentiation and proliferation, which provides a new method for the elucidation of the complex mechanisms involved in cell-parasite and cell-cell interactions during T. spiralis infection. (C) 2016 Elsevier B.V. All rights reserved. C1 [Bai, X.; Wang, X. L.; Tang, B.; Wu, X. P.; Liu, M. Y.; Liu, X. L.] Jilin Univ, Natl Inst Parasit Dis, Chinese Ctr Dis Control & Prevent, Shanghai Key Lab Zoonoses Res,Minist Educ,Inst Zo, Changchun, Peoples R China. [Shi, H. N.] Massachusetts Gen Hosp East, Pediat Gastroenterol Unit, Mucosal Immunol Lab, Boston, MA USA. [Boireau, P.] Hlth Anim Lab, ANSES, Maisons Alfort, France. [Rosenthal, B.] USDA, Anim Parasit Dis Lab, Bldg 1180, Beltsville, MD 20705 USA. [Liu, M. Y.] Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China. RP Wu, XP; Liu, MY; Liu, XL (reprint author), Jilin Univ, Natl Inst Parasit Dis, Chinese Ctr Dis Control & Prevent, Shanghai Key Lab Zoonoses Res,Minist Educ,Inst Zo, Changchun, Peoples R China. EM lvy.2001@163.com; liumy@jlu.edu.cn; liuxlei@163.com OI Rosenthal, Benjamin/0000-0002-0224-3773 NR 42 TC 0 Z9 0 U1 2 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD NOV 15 PY 2016 VL 231 SI SI BP 83 EP 91 DI 10.1016/j.vetpar.2016.07.033 PG 9 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA EG0LK UT WOS:000390724100014 PM 27501988 ER PT J AU Gamon, JA Huemmrich, KF Wong, CYS Ensminger, I Garrity, S Hollinger, DY Noormets, A Penuelas, J AF Gamon, John A. Huemmrich, K. Fred Wong, Christopher Y. S. Ensminger, Ingo Garrity, Steven Hollinger, David Y. Noormets, Asko Penuelas, Josep TI A remotely sensed pigment index reveals photosynthetic phenology in evergreen conifers SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE carotenoid pigments; evergreen conifers; gross primary productivity; chlorophyll/carotenoid index; CCl ID PHOTOCHEMICAL REFLECTANCE INDEX; RADIATION-USE EFFICIENCY; LIGHT-USE EFFICIENCY; NET ECOSYSTEM EXCHANGE; ATMOSPHERIC CO2; PHOTOSYSTEM-II; CAROTENOID COMPOSITION; MEDITERRANEAN FOREST; OPTICAL INDICATOR; SEASONAL PATTERNS AB In evergreen conifers, where the foliage amount changes little with season, accurate detection of the underlying "photosynthetic phenology" from satellite remote sensing has been difficult, presenting challenges for global models of ecosystem carbon uptake. Here, we report a close correspondence between seasonally changing foliar pigment levels, expressed as chlorophyll/carotenoid ratios, and evergreen photosynthetic activity, leading to a "chlorophyll/carotenoid index" (CCI) that tracks evergreen photosynthesis at multiple spatial scales. When calculated from NASA's Moderate Resolution Imaging Spectroradiometer satellite sensor, the CCI closely follows the seasonal patterns of daily gross primary productivity of evergreen conifer stands measured by eddy covariance. This discovery provides a way of monitoring evergreen photosynthetic activity from optical remote sensing, and indicates an important regulatory role for carotenoid pigments in evergreen photosynthesis. Improved methods of monitoring photosynthesis from space can improve our understanding of the global carbon budget in a warming world of changing vegetation phenology. C1 [Gamon, John A.] Univ Nebraska Lincoln, Sch Nat Resources, Ctr Adv Land Management Informat Technol, Lincoln, NE 68583 USA. [Gamon, John A.] Univ Alberta, Dept Earth & Atmospher Sci, Edmonton, AB T6G 2E3, Canada. [Gamon, John A.] Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada. [Huemmrich, K. Fred] Univ Maryland Baltimore Cty, NASA Goddard Space Flight Ctr, Baltimore, MD 20771 USA. [Wong, Christopher Y. S.; Ensminger, Ingo] Univ Toronto, Dept Biol, Mississauga, ON L5L 1C6, Canada. [Wong, Christopher Y. S.; Ensminger, Ingo] Univ Toronto, Grad Dept Ecol & Evolutionary Biol, Toronto, ON M5S 1A1, Canada. [Ensminger, Ingo] Univ Toronto, Grad Dept Cell & Syst Biol, Toronto, ON M5S 3G5, Canada. [Garrity, Steven] Decagon Devices Inc, Pullman, WA 99163 USA. [Hollinger, David Y.] US Forest Serv, Northern Res Stn, Durham, NH 03824 USA. [Noormets, Asko] North Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Penuelas, Josep] Ctr Ecol Res & Forestry Applicat CREAF CSIC, Global Ecol Unit, Consejo Super Invest Cient CSIC, Campus Bellaterra, Bellaterra 08193, Catalonia, Spain. [Penuelas, Josep] CREAF, Cerdanyola Del Valles 08193, Catalonia, Spain. RP Gamon, JA (reprint author), Univ Nebraska Lincoln, Sch Nat Resources, Ctr Adv Land Management Informat Technol, Lincoln, NE 68583 USA.; Gamon, JA (reprint author), Univ Alberta, Dept Earth & Atmospher Sci, Edmonton, AB T6G 2E3, Canada.; Gamon, JA (reprint author), Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada. EM jgamon@gmail.com FU Alberta Informatics Circle of Research Excellence/Alberta Innovates Technology Futures [G224150012, 200700172]; Natural Sciences and Engineering Research Council (NSERC) [RGPIN-2015-05129]; Canada Foundation for Innovation (CFI) [26793]; NASA Arctic-Boreal Vulnerability Experiment [NNX15AT78A]; NASA Science of Aqua and Terra program [NNX14AJ65G]; NSERC [RGPIN-2015-06514]; CFI [27330]; Ontario Ministry of Research and Innovation [ER10-07-015]; European Research Council Synergy Grant [SyG-2013-610028]; University of Washington [11-JV-11261952-030]; USDA FS Eastern Forest Environmental Threat Assessment Center [03-CA-11330147-073, 04-CA-11330147-238]; DOE-National Institute for Climate Change Research (NICCR) [08-SC-NICCR-1072]; Department of the Interior (DOI) Southeast Climate Science Center [G10AC00624]; DOE Terrestrial Ecosystem Science Award [11-DE-SC-0006700]; USDA NIFA [2011-68002-30185, 2014-67003-22068]; US Department of Energy (DOE)'s Office of Science; US Department of Agriculture (USDA) Forest Service Northern Research Station FX We thank Dave Landis (GST, Inc.) for MODIS data processing. J.A.G. was supported by the Alberta Informatics Circle of Research Excellence/Alberta Innovates Technology Futures (Grants G224150012 and 200700172), Natural Sciences and Engineering Research Council (NSERC) (Grant RGPIN-2015-05129), Canada Foundation for Innovation (CFI) (Grant 26793), and NASA Arctic-Boreal Vulnerability Experiment (Grant NNX15AT78A). K.F.H. was supported by the NASA Science of Aqua and Terra program (Grant NNX14AJ65G). I.E. was supported by the NSERC (Grant RGPIN-2015-06514), CFI (Grant 27330), and Ontario Ministry of Research and Innovation (Grant ER10-07-015). J.P. was supported by European Research Council Synergy Grant SyG-2013-610028 IMBALANCE-P. Data and logistical support for the Wind River site were provided by the US Forest Service (FS) Pacific Northwest Research Station and the University of Washington (Grant 11-JV-11261952-030). Support for the Howland Forest AmeriFlux core site was provided by the US Department of Energy (DOE)'s Office of Science and the US Department of Agriculture (USDA) Forest Service Northern Research Station. The work at the Parker Tract, NC (US-NC2) site was supported by USDA FS Eastern Forest Environmental Threat Assessment Center Cooperative Agreements 03-CA-11330147-073 and 04-CA-11330147-238, DOE-National Institute for Climate Change Research (NICCR) Award 08-SC-NICCR-1072, Department of the Interior (DOI) Southeast Climate Science Center Award G10AC00624, DOE Terrestrial Ecosystem Science Award 11-DE-SC-0006700, and USDA NIFA Awards 2011-68002-30185 and 2014-67003-22068. NR 56 TC 0 Z9 0 U1 23 U2 23 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD NOV 15 PY 2016 VL 113 IS 46 BP 13087 EP 13092 DI 10.1073/pnas.1606162113 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA ED6MQ UT WOS:000388970100064 PM 27803333 ER PT J AU Rathjens, H Bieger, K Chaubey, I Arnold, JG Allen, PM Srinivasan, R Bosch, DD Volk, M AF Rathjens, H. Bieger, K. Chaubey, I. Arnold, J. G. Allen, P. M. Srinivasan, R. Bosch, D. D. Volk, M. TI Delineating floodplain and upland areas for hydrologic models: a comparison of methods SO HYDROLOGICAL PROCESSES LA English DT Article DE landscape units; watershed discretization; hydrological modelling; DEM; terrain analysis; SWAT ID DIGITAL ELEVATION MODELS; COASTAL-PLAIN; CONTRIBUTING AREA; SWAT MODEL; SOIL; INFORMATION; DRAINAGE; ALGORITHMS; NETWORKS; INDEX AB A spatially distributed representation of basin hydrology and transport processes in hydrologic models facilitates the identification of critical source areas and the placement of management and conservation measures. Floodplains are critical landscape features that differ from neighbouring uplands in terms of their hydrological processes and functions. Accordingly, an important step in watershed modelling is the representation of floodplain and upland areas within a watershed. The aim of this study is (1) to evaluate four floodplain-upland delineation methods that use readily available topographic data (topographic wetness index, slope position, uniform flood stage, and variable flood stage) with regard to their suitability for hydrological models and (2) to introduce an evaluation scheme for the delineated landscape units. The methods are tested in three U.S. watersheds ranging in size from 334 to 629km(2) with different climatic, hydrological, and geomorphological characteristics. Evaluation of the landscape delineation methods includes visual comparisons, error matrices (i.e. cross-tabulations of delineated vs reference data), and geometric accuracy metrics. Reference data were obtained from the Soil Survey Geographic (SSURGO) database and Federal Emergency Management Agency (FEMA) flood maps. Results suggest that the slope position and the variable flood stage method work very well in all three watersheds. Overall percentages of floodplain and upland areas allocated correctly were obtained by comparing delineated and reference data. Values range from 83 to 93% for the slope position and from 80 to 95% for the variable flood stage method. Future studies will incorporate these two floodplain-upland delineation methods into the subwatershed-based hydrologic model Soil and Water Assessment Tool (SWAT) to improve the representation of hydrological processes within floodplain and upland areas. Copyright (c) 2016 John Wiley & Sons, Ltd. C1 [Rathjens, H.; Chaubey, I.] Purdue Univ, Earth Atmospher & Planetary Sci, 550 Stadium Mall, W Lafayette, IN 47907 USA. [Bieger, K.] Texas A&M AgriLife, Blackland Res & Extens Ctr, 720 E Blackland Rd, Temple, TX 76502 USA. [Chaubey, I.] Purdue Univ, Dept Agr & Biol Engn, 225 South Univ St, W Lafayette, IN 47907 USA. [Arnold, J. G.] ARS, Grassland Soil & Water Res Lab, USDA, 808 E Blackland Rd, Temple, TX 76502 USA. [Allen, P. M.] Baylor Univ, Dept Geol, 97354 One Bear Pl, Waco, TX 76798 USA. [Srinivasan, R.] Texas A&M Univ, Dept Ecosyst Sci & Management, 1500 Res Pkwy, College Stn, TX 77845 USA. [Srinivasan, R.] Texas A&M Univ, Dept Biol & Agr Engn, 1500 Res Pkwy, College Stn, TX 77845 USA. [Bosch, D. D.] ARS, Southeast Watershed Res Lab, USDA, POB 946, Tifton, GA 31793 USA. [Volk, M.] UFZ Leipzig, Helmholtz Ctr Environm Res, Dept Computat Landscape Ecol, Permoserstr 15, D-04318 Leipzig, Germany. RP Rathjens, H (reprint author), Purdue Univ, Earth Atmospher & Planetary Sci, 550 Stadium Mall, W Lafayette, IN 47907 USA. EM hrathjen@purdue.edu NR 85 TC 1 Z9 1 U1 4 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD NOV 15 PY 2016 VL 30 IS 23 BP 4367 EP 4383 DI 10.1002/hyp.10918 PG 17 WC Water Resources SC Water Resources GA EC7FU UT WOS:000388302900010 ER PT J AU Bledsoe, JW Peterson, BC Swanson, KS Small, BC AF Bledsoe, Jacob W. Peterson, Brian C. Swanson, Kelly S. Small, Brian C. TI Ontogenetic Characterization of the Intestinal Microbiota of Channel Catfish through 16S rRNA Gene Sequencing Reveals Insights on Temporal Shifts and the Influence of Environmental Microbes SO PLOS ONE LA English DT Article ID FRESH-WATER FISH; GUT MICROBIOTA; GASTROINTESTINAL MICROBIOTA; BACTERIAL COMMUNITY; ATLANTIC SALMON; IMMUNE-SYSTEM; DIVERSITY; ZEBRAFISH; BRADYRHIZOBIUM; TILAPIA AB Aquaculture recently overtook capture fisheries as the largest producer of food fish, but to continue increasing fish production the industry is in search of better methods of improving fish health and growth. Pre- and probiotic supplementation has gained attention as a means of solving these issues, however, for such approaches to be successful, we must first gain a more holistic understanding of the factors influencing the microbial communities present in the intestines of fish. In this study, we characterize the bacterial communities associated with the digestive tract of a highly valuable U.S. aquaculture species, channel catfish Ictalurus punctatus, over the first 193 days of life to evaluate temporal changes that may occur throughout ontogenetic development of the host. Intestinal microbiota were surveyed with high-throughput DNA sequencing of 16S rRNA V4 gene amplicons derived from fish at 3, 65, 125, and 193 days post hatch (dph), while also characterizing the environmental microbes derived from the water supply and the administered diets. Microbial communities inhabiting the intestines of catfish early in life were dynamic, with significant shifts occurring up to 125 dph when the microbiota somewhat stabilized, as shifts were less apparent between 125 to 193 dph. Bacterial phyla present in the gut of catfish throughout ontogeny include Bacteroidetes, Firmicutes, Fusobacteria, and Proteobacteria; with the species Cetobacterium somerae and Plesiomonas shigelloides showing the highest abundance in the catfish microbiota after 3 dph. Comparisons of the gut microbiota to the environmental microbes reveals that the fish gut is maintained as a niche habitat, separate from the overall microbial communities present in diets and water-supply. Although, there is also evidence that the environmental microbiota serves as an inoculum to the fish gut. Our results have implications for future research related to channel catfish biology and culture, and increase our understanding of ontogenetic effects on the microbiota of teleost fish. C1 [Bledsoe, Jacob W.] Southern Illinois Univ, Dept Anim Sci, Ctr Fisheries Aquaculture & Aquat Sci, Carbondale, IL 62901 USA. [Peterson, Brian C.] USDA ARS, Warmwater Aquaculture Res Unit, Stoneville, MS 38776 USA. [Swanson, Kelly S.] Univ Illinois, Dept Anim Sci, Urbana, IL USA. [Swanson, Kelly S.] Univ Illinois, Div Nutr Sci, Urbana, IL USA. [Bledsoe, Jacob W.; Small, Brian C.] Univ Idaho, Dept Fish & Wildlife Sci, Inst Aquaculture Res, Hagerman, ID 83332 USA. RP Small, BC (reprint author), Univ Idaho, Dept Fish & Wildlife Sci, Inst Aquaculture Res, Hagerman, ID 83332 USA. EM bcsmail@uidaho.edu FU United States Department of Agriculture, Agricultural Research Service, Warmwater Aquaculture Research Unit [6066-31000-013-00] FX The project was funded by the United States Department of Agriculture, Agricultural Research Service, Warmwater Aquaculture Research Unit (6066-31000-013-00; http://www.ars.usda/gov/research/protects/projects.htm?acc_no 428191). NR 75 TC 0 Z9 0 U1 10 U2 10 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 15 PY 2016 VL 11 IS 11 AR e0166379 DI 10.1371/journal.pone.0166379 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EC0MG UT WOS:000387794600054 PM 27846300 ER PT J AU Werner, SJ Hobson, KA Van Wilgenburg, SL Fischer, JW AF Werner, Scott J. Hobson, Keith A. Van Wilgenburg, Steven L. Fischer, Justin W. TI Multi-Isotopic (delta H-2, delta C-13, delta N-15) Tracing of Molt Origin for Red-Winged Blackbirds Associated with Agro-Ecosystems SO PLOS ONE LA English DT Article ID MIGRATORY CONNECTIVITY; WINTERING GROUNDS; NITROGEN ISOTOPES; ANIMAL MIGRATION; FEATHER ISOSCAPE; STABLE HYDROGEN; BIRDS; REPELLENT; FIDELITY; DAMAGE AB We analyzed stable-hydrogen (delta H-2), carbon (delta C-13) and nitrogen (delta N-15) isotope ratios in feathers to better understand the molt origin and food habits of Red-winged Blackbirds (Agelaius phoeniceus) near sunflower production in the Upper Midwest and rice production in the Mid-South of the United States. Outer primary feathers were used from 661 after-second-year (ASY) male blackbirds collected in Minnesota, Montana, North Dakota and South Dakota (spring collection), and Arkansas, Louisiana, Mississippi, Missouri and Texas (winter collection). The best-fit model indicated that the combination of feather delta H-2, delta C-13 and delta N-15 best predicted the state of sample collections and thus supported the use of this approach for tracing molt origins in Red-winged Blackbirds. When considering only birds collected in spring, 56% of birds were classified to their collection state on the basis of delta H-2 and delta C-13 alone. We then developed feather isoscapes for delta C-13 based upon these data and for delta H-2 based upon continental patterns of delta H-2 in precipitation. We used 81 birds collected at the ten independent sites for model validation. The spatially-explicit assignment of these 81 birds to the delta H-2 isoscape resulted in relatively high rates (similar to 77%) of accurate assignment to collection states. We also modeled the spatial extent of C3 (e.g. rice, sunflower) and C4 (corn, millet, sorghum) agricultural crops grown throughout the Upper Midwest and Mid-South United States to predict the relative use of C3-versus C4-based foodwebs among sampled blackbirds. Estimates of C3 inputs to diet ranged from 50% in Arkansas to 27% in Minnesota. As a novel contribution to blackbird conservation and management, we demonstrate how such feather isoscapes can be used to predict the molt origin and interstate movements of migratory blackbirds for subsequent investigations of breeding biology (e.g. sex-specific philopatry), agricultural depredation, feeding ecology, physiology of migration and sensitivity to environmental change. C1 [Werner, Scott J.; Fischer, Justin W.] US Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. [Hobson, Keith A.] Environm Canada, 11 Innovat Blvd, Saskatoon, SK S7N 3H5, Canada. [Hobson, Keith A.] Univ Western Ontario, Dept Biol, 1151 Richmond St N, London, ON N6A 5B7, Canada. [Van Wilgenburg, Steven L.] Environm Canada, Prairie & Northern Wildlife Res Ctr, Canadian Wildlife Serv, 115 Perimeter Rd, Saskatoon, SK S7N 0X4, Canada. RP Werner, SJ (reprint author), US Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM Scott.J.Werner@aphis.usda.gov FU USDA's National Wildlife Research Center FX SW and KH received Blue Sky Grant from USDA's National Wildlife Research Center for this study. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 48 TC 0 Z9 0 U1 5 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 15 PY 2016 VL 11 IS 11 AR e0165996 DI 10.1371/journal.pone.0165996 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EC0MG UT WOS:000387794600026 PM 27846302 ER PT J AU Lang, YK Henningson, J Jasperson, D Li, YH Lee, JW Ma, JJ Li, YH Cao, N Liu, HX Wilson, W Richt, J Ruder, M McVey, S Ma, WJ AF Lang, Yuekun Henningson, Jamie Jasperson, Dane Li, Yonghai Lee, Jinhwa Ma, Jingjiao Li, Yuhao Cao, Nan Liu, Haixia Wilson, William Richt, Juergen Ruder, Mark McVey, Scott Ma, Wenjun TI Mouse model for the Rift Valley fever virus MP12 strain infection SO VETERINARY MICROBIOLOGY LA English DT Article DE Rift Valley fever virus; Mouse model; MP12 vaccine strain ID PUNTA TORO VIRUS; IFNAR(-/-) MICE; MP-12 VACCINE; SHEEP; NSS; PATHOGENESIS; SEGMENTS; IMMUNITY; REVEALS; SAFETY AB Rift Valley fever virus (RVFV), a Category A pathogen and select agent, is the causative agent of Rift Valley fever. To date, no fully licensed vaccine is available in the U.S. for human or animal use and effective antiviral drugs have not been identified. The RVFV MP12 strain is conditionally licensed for use for veterinary purposes in the U.S. which was excluded from the select agent rule of Health and Human Services and the U.S. Department of Agriculture. The MP12 vaccine strain is commonly used in BSL-2 laboratories that is generally not virulent in mice. To establish a small animal model that can be used in a BSL-2 facility-for antiviral-drug-development, we-investigated susceptibility of six mouse strains-(129S6/SvEv, STAT-1 KO, 129S1 /SvlmJ, C57BL/6J, NZW/LacJ, BALB/c) to the MP12 virus infection via an intranasal inoculation route. Severe weight loss, obvious clinical and neurologic signs, and 50% mortality was observed in the STAT-1 KO mice, whereas the other 5 mouse strains did not display obvious and/or severe disease. Virus replication and histopathological lesions were detected in brain and liver of MP12-infected STAT-1 KO mice that developed the acute-onset hepatitis and delayed-onset encephalitis. In conclusion, the STAT-1 KO mouse strain is susceptible to MP12 virus infection, indicating that it can be used to investigate RVFV antivirals in a BSL-2 environment. (C) 2016 Elsevier B.V. All rights reserved. C1 [Lang, Yuekun; Henningson, Jamie; Li, Yonghai; Lee, Jinhwa; Ma, Jingjiao; Li, Yuhao; Cao, Nan; Liu, Haixia; Richt, Juergen; Ma, Wenjun] Kansas State Univ, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA. [Jasperson, Dane; Wilson, William; Ruder, Mark; McVey, Scott] USDA ARS, Ctr Grain & Anim Hlth Res, ABADRU, Manhattan, KS USA. [Cao, Nan] Zhongkai Univ Agr & Engn, Dept Anim Hlth, Guangzhou 510225, Guangdong, Peoples R China. [Ruder, Mark] Univ Georgia, Coll Vet Med, Dept Populat Hlth, Southeastern Cooperat Wildlife Dis Study, Athens, GA 30602 USA. RP Ma, WJ (reprint author), Kansas State Univ, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA. EM wma@vet.k-state.edu FU Kansas State University (SRO) [001]; Department of Homeland Security Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD) [2010-ST061-AG0001]; USDA Agricultural Services Project [3020-32000-005-00D]; Kansas State NBAF Transition Funds FX We would like to thank the KSVDL Histopathology and Immunohistochemistry laboratory for the processing slides and Jennifer Phinney for helping the immunohistochemistry staining. This project was supported by Kansas State University Start-Up Fund (SRO# 001) and Grants of the Department of Homeland Security Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD), Grant no. 2010-ST061-AG0001, USDA Agricultural Services Project 3020-32000-005-00D and Kansas State NBAF Transition Funds. NR 38 TC 1 Z9 1 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 EI 1873-2542 J9 VET MICROBIOL JI Vet. Microbiol. PD NOV 15 PY 2016 VL 195 BP 70 EP 77 DI 10.1016/j.vetmic.2016.09.009 PG 8 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA EB8GN UT WOS:000387629600011 PM 27771072 ER PT J AU Holsinger, L Parks, SA Miller, C AF Holsinger, Lisa Parks, Sean A. Miller, Carol TI Weather, fuels, and topography impede wildland fire spread in western US landscapes SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Fire spread; Fuel break; Environmental drivers; Rocky Mountain forests; Abiotic and biotic regulation; Wilderness ID YOSEMITE-NATIONAL-PARK; MIXED-CONIFER FOREST; BOREAL FOREST; WILDERNESS AREAS; SIERRA-NEVADA; LOGISTIC-REGRESSION; SPATIAL CONTROLS; UNITED-STATES; REGIMES; CLIMATE AB As wildland fire activity continues to surge across the western US, it is increasingly important that we understand and quantify the environmental drivers of fire and how they vary across ecosystems. At daily to annual timescales, weather, fuels, and topography are known to influence characteristics such as area burned and fire severity. An understudied facet, however, concerns how these factors inhibit fire spread and thereby contribute to the formation of fire boundaries. We evaluated how weather, fuels, and topography impeded fire spread in four large study areas in the western US, three in the Northern Rockies and one in the Southwest. Weather and fuels were the most important factors in the Northern Rockies, whereas fuels and topography were dominant in the Southwest. Within the categories of weather, fuels, and topography, we also evaluated which specific variables were most influential in impeding fire spread. We explicitly accounted for the presence and age of previous burns within the fuels category. We found that: (1) temperature was the most influential weather variable in the Northern Rockies; (2) previous burns (particularly those that were <= 5 years old) were moderately to highly influential in all study areas; and (3) valley bottoms and ridgetops were moderately to highly associated with fire boundaries in all study areas. Our results elucidate the regionally varying roles of weather, fuels, and topography in impeding fire spread, emphasizing each ecosystem's unique biophysical setting and fire regime. Published by Elsevier B.V. C1 [Holsinger, Lisa; Parks, Sean A.; Miller, Carol] US Forest Serv, USDA, Rocky Mt Res Stn, Aldo Leopold Wilderness Inst, 790 East Beckwith, Missoula, MT 59801 USA. RP Holsinger, L (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Aldo Leopold Wilderness Inst, 790 East Beckwith, Missoula, MT 59801 USA. EM lisamholsinger@fs.fed.us FU Rocky Mountain Research Station [12-1-03-19] FX We acknowledge the Joint Fire Science Program under JFSP Project 12-1-03-19, as well as National Fire Plan Funding from the Rocky Mountain Research Station. NR 108 TC 1 Z9 1 U1 25 U2 25 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD NOV 15 PY 2016 VL 380 BP 59 EP 69 DI 10.1016/j.foreco.2016.08.035 PG 11 WC Forestry SC Forestry GA DZ1NQ UT WOS:000385605500007 ER PT J AU Liechty, HO Hooper, JJ AF Liechty, Hal O. Hooper, Jennifer J. TI Long-term effect of periodic fire on nutrient pools and soil chemistry in loblolly-shortleaf pine stands managed with single-tree selection SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Prescribed fire; Uneven-aged management; Nutrient regime; Forest floor ID SOUTHEASTERN UNITED-STATES; UNEVEN-AGED STANDS; PRESCRIBED FIRE; NITROGEN MINERALIZATION; HERBACEOUS VEGETATION; SOUTH-CAROLINA; GROWING-SEASON; COASTAL-PLAIN; FOREST TREES; TAEDA L AB Prescribed fire can be an important tool for managing loblolly (Pinus taeda L.) and shortleaf pine (Pinus enchinata Mill.) stands in the southern United States but few studies have reported the impact of repeated prescribed fires in stands that are routinely thinned or partially harvested. We compared nutrient pools and soil chemistry in portions of three uneven-aged loblolly-shortleaf pine stands that had received dormant-season prescribed fires every one to four years (high frequency fire) during a 20 year period to portions of these same stands in which fire was excluded (control). The stands had been managed using single-tree selection and harvested on a 5-year cycle since the establishment of the prescribed fire treatments. Sixteen months following the last prescribed fire, forest floor nutrient pools were 42-49% less in the high frequency fire than control treatment. Understory vegetation recovered rapidly and approximately 34 months following the last fire, understory biomass and nutrient pools were similar within the two treatments. However, the repeated use of prescribed fire effectively reduced the density of saplings which resulted in a 78-85% reduction in sapling biomass and nutrient pools in the high frequency fire compared to the control treatment. Chemistry of the surface (15 cm) mineral soil was similar in the two treatments indicating that the prescribed fires had little impact on the mineral soil. Periodic harvesting may have ameliorated changes in mineral soil P, Ca, or Mg availability that have been reported to occur with long-term, repeated application of prescribed fires in southern pine forests. Reductions in forest floor and sapling nutrient pools associated with the prescribed fire likely had little impact on tree productivity or soil sustainability since foliar nutrient concentrations of the overstory trees in the two treatments did not differ and the potential losses were small compared to surface soil pools. (C) 2016 Elsevier B.V. All rights reserved. C1 [Liechty, Hal O.; Hooper, Jennifer J.] Univ Arkansas Monticello, Sch Forestry & Nat Resources, 110 Univ Ct, Monticello, AR 71656 USA. [Hooper, Jennifer J.] US Forest Serv, USDA, Angeles Natl Forest, 701 N Santa Anita Ave, Arcadia, CA 91006 USA. RP Liechty, HO (reprint author), Univ Arkansas Monticello, Sch Forestry & Nat Resources, 110 Univ Ct, Monticello, AR 71656 USA. EM liechty@uamont.edu; jhooper02@fs.fed.us FU United States Department of Agriculture Forest Service Southern Research Station; Arkansas Forest Resources Center FX This research was supported by the United States Department of Agriculture Forest Service Southern Research Station and the Arkansas Forest Resources Center. We would like to thank Michael G. Shelton who was a key contributor to the development of this research project and Michele Reinke for assisting in the field work associated with the project. NR 55 TC 0 Z9 0 U1 3 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD NOV 15 PY 2016 VL 380 BP 252 EP 260 DI 10.1016/j.foreco.2016.08.045 PG 9 WC Forestry SC Forestry GA DZ1NQ UT WOS:000385605500026 ER PT J AU Vose, JM Clark, JS Luce, CH AF Vose, James M. Clark, James S. Luce, Charles H. TI Introduction to drought and US forests: Impacts and potential management responses SO FOREST ECOLOGY AND MANAGEMENT LA English DT Editorial Material ID UNITED-STATES; LAST MILLENNIUM; CLIMATE-CHANGE; DIE-OFF; MORTALITY; WILDFIRE; CONSEQUENCES; HEALTH C1 [Vose, James M.] US Forest Serv, USDA, Southern Res Stn, Ctr Integrated Forest Sci,Dept Forestry & Environ, Raleigh, NC 27695 USA. [Clark, James S.] Duke Univ, Nicholas Sch Environm, Environm Sci, Durham, NC 27708 USA. [Luce, Charles H.] US Forest Serv, USDA, Rocky Mt Res Stn, Boise, ID 83702 USA. RP Vose, JM (reprint author), US Forest Serv, USDA, Southern Res Stn, Ctr Integrated Forest Sci,Dept Forestry & Environ, Raleigh, NC 27695 USA. RI Luce, Charles/A-9267-2008 OI Luce, Charles/0000-0002-6938-9662 NR 33 TC 0 Z9 0 U1 17 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD NOV 15 PY 2016 VL 380 BP 296 EP 298 DI 10.1016/j.foreco.2016.09.030 PG 3 WC Forestry SC Forestry GA DZ1NQ UT WOS:000385605500030 ER PT J AU Luce, CH Vose, JM Pederson, N Campbell, J Millar, C Kormos, P Woods, R AF Luce, Charles H. Vose, James M. Pederson, Neil Campbell, John Millar, Connie Kormos, Patrick Woods, Ross TI Contributing factors for drought in United States forest ecosystems under projected future climates and their uncertainty SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Drought; Forests; Climate change; Projections; Climate modes ID INDUCED TREE MORTALITY; NORTH-AMERICAN CLIMATE; HYDROCLIMATE VARIABILITY; VEGETATION MORTALITY; CHANGE IMPACTS; DIE-OFF; WATER; PRECIPITATION; TEMPERATURE; MECHANISMS AB Observations of increasing global forest die-off related to drought are leading to more questions about potential increases in drought occurrence, severity, and ecological consequence in the future. Dry soils and warm temperatures interact to affect trees during drought; so understanding shifting risks requires some understanding of changes in both temperature and precipitation. Unfortunately, strong precipitation uncertainties in climate models yield substantial uncertainty in projections of drought occurrence. We argue that disambiguation of drought effects into temperature and precipitation-mediated processes can alleviate some of the implied uncertainty. In particular, the disambiguation can clarify geographic diversity in forest sensitivity to multifarious drivers of drought and mortality, making more specific use of geographically diverse climate projections. Such a framework may provide forest managers with an easier heuristic in discerning geographically diverse adaptation options. Warming temperatures in the future mean three things with respect to drought in forests: (1) droughts, typically already unusually hot periods, will become hotter, (2) the drying capacity of the air, measured as the vapor pressure deficit (VPD) will become greater, and (3) a smaller fraction of precipitation will fall as snow. More hot temperature extremes will be more stressful in a direct way to living tissue, and greater VPD will increase pressure gradients within trees, exacerbating the risk of hydraulic failure. Reduced storage in snowpacks reduces summer water availability in some places. Warmer temperatures do not directly cause drier soils, however. In a hydrologic sense, warmer temperatures do little to cause "drought" as defined by water balances. Instead, much of the future additional longwave energy flux is expected to cause warming rather than evaporating water. Precipitation variations, in contrast, affect water balances and moisture availability directly; so uncertainties in future precipitation generate uncertainty in drought occurrence and severity projections. Although specific projections in annual and seasonal precipitation are uncertain, changes in inter-storm spacing and precipitation type (snow vs. rain) have greater certainty and may have utility in improving spatial projections of drought as perceived by vegetation, a value not currently captured by simple temperature-driven evaporation projections. This review ties different types of future climate shifts to expected consequences for drought and potential influences on physiology, and then explains sources of uncertainty for consideration in future mortality projections. One intention is to provide guidance on partitioning of uncertainty in projections of forest stresses. Published by Elsevier B.V. C1 [Luce, Charles H.] US Forest Serv, USDA, Rocky Mt Res Stn, Boise, ID USA. [Vose, James M.] US Forest Serv, USDA, Southern Res Stn, Boise, ID USA. [Pederson, Neil] Harvard Forest, Petersham, MA USA. [Campbell, John] US Forest Serv, USDA, Northern Res Stn, Durham, NH USA. [Millar, Connie] US Forest Serv, USDA, Souothwestern Res Stn, Albany, CA USA. [Kormos, Patrick] US Forest Serv, USDA, Pacific Southwest Res Stn, Albany, CA USA. [Woods, Ross] Univ Bristol, Fac Engn, Bristol, Avon, England. RP Luce, CH (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Boise, ID USA. EM cluce@fs.fed.us RI Woods, Ross/C-6696-2013; Luce, Charles/A-9267-2008 OI Woods, Ross/0000-0002-5732-5979; Luce, Charles/0000-0002-6938-9662 NR 115 TC 4 Z9 4 U1 45 U2 45 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD NOV 15 PY 2016 VL 380 BP 299 EP 308 DI 10.1016/j.foreco.2016.05.020 PG 10 WC Forestry SC Forestry GA DZ1NQ UT WOS:000385605500031 ER PT J AU Kolb, TE Fettig, CJ Ayres, MP Bentz, BJ Hicke, JA Mathiasen, R Stewart, JE Weed, AS AF Kolb, Thomas E. Fettig, Christopher J. Ayres, Matthew P. Bentz, Barbara J. Hicke, Jeffrey A. Mathiasen, Robert Stewart, Jane E. Weed, Aaron S. TI Observed and anticipated impacts of drought on forest insects and diseases in the United States SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Climate change; Drought; Forest pathogens; Forest health; Forest insects; Herbivory ID MOUNTAIN PINE-BEETLE; WESTERN SPRUCE BUDWORM; PINYON-JUNIPER WOODLANDS; SWISS NEEDLE CAST; FUEL-REDUCTION TREATMENTS; ADELGID ADELGES-TSUGAE; SUPPORT CURRENT POLICY; MIXED-CONIFER FORESTS; CLIMATE-CHANGE; PONDEROSA PINE AB Future anthropogenic-induced changes to the earth's climate will likely include increases in temperature and changes in precipitation that will increase the frequency and severity of droughts. Insects and fungal diseases are important disturbances in forests, yet understanding of the role of drought in outbreaks of these agents is limited. Current knowledge concerning the effects of drought on herbivorous insect and pathogen outbreaks in U.S. forests is reviewed, and compared between the relatively mesic and structurally diverse forests of the eastern U.S. and the more xeric forests of the western U.S. Theory and limited evidence suggests a non-linear relationship between drought intensity and outbreaks of aggressive bark beetle species (i.e., those capable of causing extensive levels of tree mortality), where moderate droughts reduce bark beetle population performance and subsequent tree mortality, whereas intense droughts, which frequently occur in the western U.S., increase bark beetle performance and tree mortality. There is little evidence for a role of drought in outbreaks of the southern pine beetle (Dendroctonus frontalis), the only bark beetle species that causes large amounts of tree mortality in the eastern U.S. Defoliators do not show consistent responses to drought. The response of sapfeeders to drought appears non-linear, with the greatest performance and impacts at intermediate drought intensity or when drought is alleviated by wetter periods. Interactions between tree pathogens and drought are poorly understood, but available evidence suggests reduced pathogen performance and host impacts in response to drought for primary pathogens and pathogens whose lifecycle depends directly on moisture (humidity). In these cases, rates of reproduction, spread, and infection tend to be greater when conditions are moist. In contrast, secondary fungal pathogens (i.e., those that depend on stressed hosts for colonization) are anticipated to respond to drought with greater performance and host impacts. In the western U.S., drought increases stress on trees severely infected by mistletoes thereby predisposing mistletoe-infected trees to attack by insects, particularly bark beetles and wood borers. Research needed to advance understanding of drought impacts on forest insects and diseases, and the role of forest management in mitigation of infestations during drought are discussed. (C) 2016 Elsevier B.V. All rights reserved. C1 [Kolb, Thomas E.; Mathiasen, Robert] No Arizona Univ, Sch Forestry, Flagstaff, AZ 86011 USA. [Fettig, Christopher J.] US Forest Serv, Pacific Southwest Res Stn, USDA, Davis, CA USA. [Ayres, Matthew P.] Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA. [Bentz, Barbara J.] US Forest Serv, Rocky Mt Res Stn, USDA, Logan, UT USA. [Hicke, Jeffrey A.] Univ Idaho, Dept Geog, Moscow, ID 83843 USA. [Stewart, Jane E.] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. [Weed, Aaron S.] MidAtlantic Network, Natl Pk Serv, Inventory & Monitoring Program, Fredericksburg, VA USA. RP Kolb, TE (reprint author), No Arizona Univ, Sch Forestry, Flagstaff, AZ 86011 USA. EM tom.kolb@nau.edu; cfettig@fs.fed.us; Matthew.P.Ayres@dartmouth.edu; bj.bentz@gmail.com; jhicke@uidaho.edu; robert.mathiasen@nau.edu; Stewart@colostate.edu; aaron_weed@nps.gov NR 237 TC 6 Z9 6 U1 61 U2 61 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD NOV 15 PY 2016 VL 380 BP 321 EP 334 DI 10.1016/j.foreco.2016.04.051 PG 14 WC Forestry SC Forestry GA DZ1NQ UT WOS:000385605500033 ER PT J AU Vose, JM Miniat, CF Luce, CH Asbjornsen, H Caldwell, PV Campbell, JL Grant, GE Isaak, DJ Loheide, SP Sun, G AF Vose, James M. Miniat, Chelcy Ford Luce, Charles H. Asbjornsen, Heidi Caldwell, Peter V. Campbell, John L. Grant, Gordon E. Isaak, Daniel J. Loheide, Steven P., II Sun, Ge TI Ecohydrological implications of drought for forests in the United States SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Transpiration; Streamflow; Water balance; Water quality; Climate change; Management options ID DISSOLVED ORGANIC-CARBON; US NATIONAL FORESTS; CLIMATE-CHANGE; WATER YIELD; SOUTHERN APPALACHIANS; STREAM TEMPERATURES; TREE MORTALITY; MANAGEMENT IMPLICATIONS; INDUCED ACIDIFICATION; ECOSYSTEM FUNCTIONS AB The relationships among drought, surface water flow, and groundwater recharge are not straightforward for most forest ecosystems due to the strong role that vegetation plays in the forest water balance. Hydrologic responses to drought can be either mitigated or exacerbated by forest vegetation depending upon vegetation water use and how forest population dynamics respond to drought. Understanding how drought impacts ecosystems requires understanding how drought impacts ecohydrological processes. Because different species and functional groups vary in their ecophysiological traits that influence water use patterns, changes in species assemblages can alter hydrological processes from the stand to the watershed scales. Recent warming trends and more prolonged and frequent droughts have accelerated the spread and intensity of insect attacks in the western US that kill nearly all of the canopy trees within forest stands, changing the energy balance of the land surface and affecting many hydrologic processes. In contrast, some eastern forest tree species and size classes can tolerate drought better than others, suggesting the potential for drought-mediated shifts in both species composition and structure. Predicting how these changes will impact hydrologic processes at larger spatial and temporal scales presents a considerable challenge. The biogeochemical consequences of drought, such as changes in stream chemistry, are closely linked to vegetation dynamics and hydrologic responses. As with other natural disturbances, droughts are difficult to prepare for because they are unpredictable. However, there are management options that may be implemented to minimize the impacts of drought on water quantity and quality. Examples include reducing leaf area by thinning and regenerating cut forests with species that consume less water, although a high level of uncertainty in both drought projections and anticipated responses suggests the need for monitoring and adaptive management. Published by Elsevier B.V. C1 [Vose, James M.] US Forest Serv, USDA, Southern Res Stn, Ctr Integrated Forest Sci,Dept Forestry & Environ, Raleigh, NC 27695 USA. [Miniat, Chelcy Ford; Caldwell, Peter V.] US Forest Serv, USDA, Southern Res Stn, Ctr Forest Watershed Res,Coweeta Hydrol Lab, Otto, NC 28763 USA. [Luce, Charles H.; Isaak, Daniel J.] US Forest Serv, USDA, Rocky Mt Res Stn, Boise, ID 83702 USA. [Asbjornsen, Heidi] Univ New Hampshire, Dept Nat Resources, Durham, NH 03824 USA. [Campbell, John L.] US Forest Serv, USDA, Northern Res Stn, Ctr Res Ecosyst Change, Durham, NH 03824 USA. [Grant, Gordon E.] US Forest Serv, USDA, Pacific Northwest Res Stn, Ecol Proc & Funct Program,Forestry Sci Lab, Corvallis, OR 97331 USA. [Loheide, Steven P., II] Univ Wisconsin, Civil & Environm Engn, Madison, WI 53706 USA. [Sun, Ge] US Forest Serv, USDA, Southern Res Stn, Eastern Forest Environm Threat Assessment Ctr, Raleigh, NC 27695 USA. RP Vose, JM (reprint author), US Forest Serv, USDA, Southern Res Stn, Ctr Integrated Forest Sci,Dept Forestry & Environ, Raleigh, NC 27695 USA. EM jvose@fs.fed.us RI Luce, Charles/A-9267-2008 OI Luce, Charles/0000-0002-6938-9662 FU USDA Forest Service, Southern Research Station FX This project was supported by the USDA Forest Service, Southern Research Station. We thank two anonymous reviewers for helpful comments on a previous version of this manuscript. NR 164 TC 4 Z9 4 U1 28 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD NOV 15 PY 2016 VL 380 BP 335 EP 345 DI 10.1016/j.foreco.2016.03.025 PG 11 WC Forestry SC Forestry GA DZ1NQ UT WOS:000385605500034 ER PT J AU Norman, SP Koch, FH Hargrove, WW AF Norman, Steven P. Koch, Frank H. Hargrove, William W. TI Review of broad-scale drought monitoring of forests: Toward an integrated data mining approach SO FOREST ECOLOGY AND MANAGEMENT LA English DT Review DE Drought impacts; Forests; Drought indices; Remote sensing; Big data; Monitoring ID CONTERMINOUS UNITED-STATES; TREE MORTALITY; GLOBAL-CHANGE; CLIMATE-CHANGE; DIE-OFF; AMAZON FORESTS; ECOLOGICAL RESILIENCE; VEGETATION INDEX; CANOPY STRUCTURE; SEVERITY INDEX AB Efforts to monitor the broad-scale impacts of drought on forests often come up short. Drought is a direct stressor of forests as well as a driver of secondary disturbance agents, making a full accounting of drought impacts challenging. General impacts can be inferred from moisture deficits quantified using precipitation and temperature measurements. However, derived meteorological indices may not meaningfully capture drought impacts because drought responses can differ substantially among species, sites and regions. Meteorology-based approaches also require the characterization of current moisture conditions relative to some specified time and place, but defining baseline conditions over large, ecologically diverse regions can be as difficult as quantifying the moisture deficit itself. In contrast, remote sensing approaches attempt to observe immediate, secondary, and longer-term changes in vegetation response, yet they too are no panacea. Remote sensing methods integrate responses across entire mixed-vegetation pixels and rarely distinguish the effects of drought on a single species, nor can they disentangle drought effects from those caused by various other disturbance agents. Establishment of suitable baselines from remote sensing may be even more challenging than with meteorological data. Here we review broad scale drought monitoring methods, and suggest that an integrated data-mining approach may hold the most promise for enhancing our ability to resolve drought impacts on forests. A big-data approach that integrates meteorological and remotely sensed data streams, together with other datasets such as vegetation type, wildfire occurrence and pest activity, can clarify direct drought effects while filtering indirect drought effects and consequences. This strategy leverages the strengths of meteorology-based and remote sensing approaches with the aid of ancillary data, such that they complement each other and lead toward a better understanding of drought impacts. Published by Elsevier B.V. C1 [Norman, Steven P.; Hargrove, William W.] US Forest Serv, USDA, Southern Res Stn, Eastern Forest Environm Threat Assessment Ctr, 200 WT Weaver Blvd, Asheville, NC 28804 USA. [Koch, Frank H.] US Forest Serv, USDA, Southern Res Stn, Eastern Forest Environm Threat Assessment Ctr, 3041 E Cornwallis Rd, Res Triangle Pk, NC 27709 USA. RP Norman, SP (reprint author), US Forest Serv, USDA, Southern Res Stn, Eastern Forest Environm Threat Assessment Ctr, 200 WT Weaver Blvd, Asheville, NC 28804 USA. EM stevenorman@fs.fed.us OI Koch, Frank/0000-0002-3750-4507 NR 135 TC 1 Z9 1 U1 43 U2 43 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD NOV 15 PY 2016 VL 380 BP 346 EP 358 DI 10.1016/j.foreco.2016.06.027 PG 13 WC Forestry SC Forestry GA DZ1NQ UT WOS:000385605500035 ER PT J AU John, KMM Natarajan, S Luthria, DL AF John, K. M. Maria Natarajan, Savithiry Luthria, Devanand L. TI Metabolite changes in nine different soybean varieties grown under field and greenhouse conditions SO FOOD CHEMISTRY LA English DT Article DE Cultivars; Greenhouse; Metabolites; Multivariate analyses; Soybean ID PHAKOPSORA-PACHYRHIZI; ISOFLAVONE CONTENT; MASS SPECTROMETRY; MAIZE HYBRIDS; FERMENTATION; DROUGHT; ACID; CHEONGGUKJANG; RESISTANCE; RESPONSES AB Global food security remains a worldwide concern due to changing climate, increasing population, and reduced agriculture acreages. Greenhouse cultivation increases productivity by extending growing seasons, reducing pest infestations and providing protection against short term drastic weather fluctuations like frost, heat, rain, and wind. In the present study, we examined and compared the metabolic responses of nine soybean varieties grown under field and greenhouse conditions. Extracts were assayed by GC-FID, GC-MS, and LC-MS for the identification of 10 primary (amino acids, organic acids, and sugars) and 10 secondary (isoflavones, fatty acid methyl esters) metabolites. Sugar molecules (glucose, sucrose, and pinitol) and isoflavone aglycons were increased but the isoflavones glucoside content decreased in the greenhouse cultivated soybeans. The amino acids and organic acids varied between the varieties. The results show that clustering (PCA and PLS-DA) patterns of soybean metabolites were significantly influenced by the genetic variation and growing conditions. Published by Elsevier Ltd. C1 [John, K. M. Maria; Luthria, Devanand L.] ARS, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. [Natarajan, Savithiry] ARS, Soybean Genom & Improvement Lab, Inst Plant Sci, USDA, Beltsville, MD 20705 USA. RP Natarajan, S (reprint author), ARS, Soybean Genom & Improvement Lab, Inst Plant Sci, USDA, Beltsville, MD 20705 USA.; Luthria, DL (reprint author), ARS, Food Composit Methods Dev Lab, BARC E, USDA, Bldg 161,Lab 202,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Dave.Luthria@ars.usda.gov FU ARS projects FX Funding for this research was provided by ARS projects. Mention of trade name, proprietary product or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture or imply its approval to the exclusion of other products or vendors that also may be suitable. NR 37 TC 1 Z9 1 U1 73 U2 151 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD NOV 15 PY 2016 VL 211 BP 347 EP 355 DI 10.1016/j.foodchem.2016.05.055 PG 9 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA DO1MW UT WOS:000377543500043 ER PT J AU Ai, YF Cichy, KA Harte, JB Kelly, JD Ng, PKW AF Ai, Yongfeng Cichy, Karen A. Harte, Janice B. Kelly, James D. Ng, Perry K. W. TI Effects of extrusion cooking on the chemical composition and functional properties of dry common bean powders SO FOOD CHEMISTRY LA English DT Article DE Common beans; Extrusion cooking; Bean powder; Chemical composition; Functional properties; Starch digestibility ID RESISTANT STARCH CONTENTS; PHASEOLUS-VULGARIS; WHEAT-FLOUR; PHYSICOCHEMICAL PROPERTIES; ANTINUTRITIONAL FACTORS; LEGUMES; FABA; DIGESTIBILITY; CONSUMPTION; CAPACITIES AB The impact of extrusion cooking on the chemical composition and functional properties of bean powders from four common bean varieties was investigated. The raw bean powders were extruded under eight different conditions, and the extrudates were then dried and ground (particle size <= 0.5 mm). Compared with corresponding non-extruded (raw) bean powders (particle size <= 0.5 mm), the extrusion treatments did not substantially change the protein and starch contents of the bean powders and showed inconsistent effects on the sucrose, raffinose and stachyose contents. The extrusion cooking did cause complete starch gelatinization and protein denaturation of the bean powders and thus changed their pasting properties and solvent-retention capacities. The starch digestibilities of the cooked non-extruded and cooked extruded bean powders were comparable. The extruded bean powders displayed functional properties similar to those of two commercial bean powders. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Ai, Yongfeng; Harte, Janice B.; Ng, Perry K. W.] Michigan State Univ, Dept Food Sci & Human Nutr, E Lansing, MI 48824 USA. [Cichy, Karen A.] ARS, USDA, Sugarbeet & Bean Res Unit, E Lansing, MI 48824 USA. [Cichy, Karen A.; Kelly, James D.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. RP Ng, PKW (reprint author), Michigan State Univ, Dept Food Sci & Human Nutr, E Lansing, MI 48824 USA. EM ngp@msu.edu FU Michigan Department of Agriculture & Rural Development Strategic Growth Initiative; Michigan Bean Commission FX The authors are grateful for the financial support from the Michigan Department of Agriculture & Rural Development Strategic Growth Initiative and the Michigan Bean Commission. NR 40 TC 1 Z9 2 U1 34 U2 70 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD NOV 15 PY 2016 VL 211 BP 538 EP 545 DI 10.1016/j.foodchem.2016.05.095 PG 8 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA DO1MW UT WOS:000377543500065 PM 27283664 ER PT J AU Nwugo, CC Doud, MS Duan, YP Lin, H AF Nwugo, Chika C. Doud, Melissa S. Duan, Yong-ping Lin, Hong TI Proteomics analysis reveals novel host molecular mechanisms associated with thermotherapy of 'Ca. Liberibacter asiaticus'-infected citrus plants SO BMC PLANT BIOLOGY LA English DT Article DE Citrus; Proteomics; Huanglongbing; Host response; Protein extraction; Chaperones; Defense-related proteins; Heat treatment; Photosynthesis-related proteins ID HUANGLONGBING DISEASE; DEFENSE RESPONSES; SACCHAROMYCES-CEREVISIAE; DISTINCT FUNCTIONS; DIAPHORINA-CITRI; VIRUS DISEASES; PHLOEM LECTIN; UP-REGULATION; MOSAIC-VIRUS; PROTEIN AB Background: Citrus Huanglongbing (HLB), which is linked to the bacterial pathogen 'Ca. Liberibacter asiaticus' (Las), is the most devastating disease of citrus plants, and longer-term control measures via breeding or genetic engineering have been unwieldy because all cultivated citrus species are susceptible to the disease. However, the degree of susceptibility varies among citrus species, which has prompted efforts to identify potential Las resistance/tolerance- related genes in citrus plants for application in breeding or genetic engineering programs. Plant exposure to one form of stress has been shown to serendipitously induce innate resistance to other forms of stress and a recent study showed that continuous heat treatment (40 to 42 degrees C) reduced Las titer and HLB-associated symptoms in citrus seedlings. The goal of the present study was to apply comparative proteomics analysis via 2-DE and mass spectrometry to elucidate the molecular processes associated with heat-induced mitigation of HLB in citrus plants. Healthy or Las-infected citrus grapefruit plants were exposed to room temperature or to continuous heat treatment of 40 degrees C for 6 days. Results: An exhaustive total protein extraction process facilitated the identification of 107 differentially-expressed proteins in response to Las and/or heat treatment, which included a strong up-regulation of chaperones including small (23.6, 18.5 and 17.9 kDa) heat shock proteins, a HSP70-like protein and a ribulose-1,5-bisphosphate carboxylase oxygenase (RuBisCO)-binding 60 kDa chaperonin, particularly in response to heat treatment. Other proteins that were generally down-regulated due to Las infection but up-regulated in response to heat treatment include RuBisCO activase, chlorophyll a/b binding protein, glucosidase II beta subunit-like protein, a putative lipoxygenase protein, a ferritin-like protein, and a glutathione S-transferase. Conclusions: The differentially-expressed proteins identified in this study highlights a premier characterization of the molecular mechanisms potentially involved in the reversal of Las-induced pathogenicity processes in citrus plants and are hence proposed targets for application towards the development of cisgenic Las-resistant/tolerant citrus plants. C1 [Nwugo, Chika C.; Lin, Hong] ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. [Doud, Melissa S.; Duan, Yong-ping] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP Lin, H (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM hong.lin@ars.usda.gov FU USDA, Agricultural Research Service FX Funding for this project was provided by the USDA, Agricultural Research Service. Trade names or commercial products in this publication are mentioned solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. USDA is an equal opportunity provider and employer. NR 86 TC 0 Z9 0 U1 9 U2 9 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD NOV 14 PY 2016 VL 16 AR 253 DI 10.1186/s12870-016-0942-x PG 15 WC Plant Sciences SC Plant Sciences GA EE3IP UT WOS:000389481300002 PM 27842496 ER PT J AU McKenna, DD Scully, ED Pauchet, Y Hoover, K Kirsch, R Geib, SM Mitchell, RF Waterhouse, RM Ahn, SJ Arsala, D Benoit, JB Blackmon, H Bledsoe, T Bowsher, JH Busch, A Calla, B Chao, H Childers, AK Childers, C Clarke, DJ Cohen, L Demuth, JP Dinh, H Doddapaneni, H Dolan, A Duan, JJ Dugan, S Friedrich, M Glastad, KM Goodisman, MAD Haddad, S Han, Y Hughes, DST Ioannidis, P Johnston, JS Jones, JW Kuhn, LA Lance, DR Lee, CY Lee, SL Lin, H Lynch, JA Moczek, AP Murali, SC Muzny, DM Nelson, DR Palli, SR Panfilio, KA Pers, D Poelchau, MF Quan, HH Qu, JX Ray, AM Rinehart, JP Robertson, HM Roehrdanz, R Rosendale, AJ Shin, S Silva, C Torson, AS Jentzsch, IMV Werren, JH Worley, KC Yocum, G Zdobnov, EM Gibbs, RA Richards, S AF McKenna, Duane D. Scully, Erin D. Pauchet, Yannick Hoover, Kelli Kirsch, Roy Geib, Scott M. Mitchell, Robert F. Waterhouse, Robert M. Ahn, Seung-Joon Arsala, Deanna Benoit, Joshua B. Blackmon, Heath Bledsoe, Tiffany Bowsher, Julia H. Busch, Andre Calla, Bernarda Chao, Hsu Childers, Anna K. Childers, Christopher Clarke, Dave J. Cohen, Lorna Demuth, Jeffery P. Dinh, Huyen Doddapaneni, HarshaVardhan Dolan, Amanda Duan, Jian J. Dugan, Shannon Friedrich, Markus Glastad, Karl M. Goodisman, Michael A. D. Haddad, Stephanie Han, Yi Hughes, Daniel S. T. Ioannidis, Panagiotis Johnston, J. Spencer Jones, Jeffery W. Kuhn, Leslie A. Lance, David R. Lee, Chien-Yueh Lee, Sandra L. Lin, Han Lynch, Jeremy A. Moczek, Armin P. Murali, Shwetha C. Muzny, Donna M. Nelson, David R. Palli, Subba R. Panfilio, Kristen A. Pers, Dan Poelchau, Monica F. Quan, Honghu Qu, Jiaxin Ray, Ann M. Rinehart, Joseph P. Robertson, Hugh M. Roehrdanz, Richard Rosendale, Andrew J. Shin, Seunggwan Silva, Christian Torson, Alex S. Jentzsch, Iris M. Vargas Werren, John H. Worley, Kim C. Yocum, George Zdobnov, Evgeny M. Gibbs, Richard A. Richards, Stephen TI Genome of the Asian longhorned beetle (Anoplophora glabripennis), a globally significant invasive species, reveals key functional and evolutionary innovations at the beetle-plant interface SO GENOME BIOLOGY LA English DT Article DE Chemoperception; Detoxification; Glycoside hydrolase; Horizontal gene transfer; Phytophagy; Xylophagy ID DENDROCTONUS-PONDEROSAE HOPKINS; MOUNTAIN PINE-BEETLE; CERAMBYCID BEETLE; GUT MICROBES; TRIBOLIUM-CASTANEUM; HORNED BEETLE; DRAFT GENOME; COLEOPTERA; CELLULASE; GENES AB Background: Relatively little is known about the genomic basis and evolution of wood- feeding in beetles. We undertook genome sequencing and annotation, gene expression assays, studies of plant cell wall degrading enzymes, and other functional and comparative studies of the Asian longhorned beetle, Anoplophora glabripennis, a globally significant invasive species capable of inflicting severe feeding damage on many important tree species. Complementary studies of genes encoding enzymes involved in digestion of woody plant tissues or detoxification of plant allelochemicals were undertaken with the genomes of 14 additional insects, including the newly sequenced emerald ash borer and bull-headed dung beetle. Results: The Asian longhorned beetle genome encodes a uniquely diverse arsenal of enzymes that can degrade the main polysaccharide networks in plant cell walls, detoxify plant allelochemicals, and otherwise facilitate feeding on woody plants. It has the metabolic plasticity needed to feed on diverse plant species, contributing to its highly invasive nature. Large expansions of chemosensory genes involved in the reception of pheromones and plant kairomones are consistent with the complexity of chemical cues it uses to find host plants and mates. Conclusions: Amplification and functional divergence of genes associated with specialized feeding on plants, including genes originally obtained via horizontal gene transfer from fungi and bacteria, contributed to the addition, expansion, and enhancement of the metabolic repertoire of the Asian longhorned beetle, certain other phytophagous beetles, and to a lesser degree, other phytophagous insects. Our results thus begin to establish a genomic basis for the evolutionary success of beetles on plants. C1 [McKenna, Duane D.; Clarke, Dave J.; Haddad, Stephanie; Shin, Seunggwan] Univ Memphis, Dept Biol Sci, 3700 Walker Ave, Memphis, TN 38152 USA. [McKenna, Duane D.] Univ Memphis, Feinstone Ctr Genom Res, Memphis, TN 38152 USA. [Scully, Erin D.] ARS, USDA, Ctr Grain & Anim Hlth, Stored Prod Insect & Engn Res Unit, Manhattan, KS 66502 USA. [Pauchet, Yannick; Kirsch, Roy; Ahn, Seung-Joon; Busch, Andre] Max Planck Inst Chem Ecol, Dept Entomol, D-07745 Jena, Germany. [Hoover, Kelli] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. [Hoover, Kelli] Penn State Univ, Ctr Chem Ecol, University Pk, PA 16802 USA. [Geib, Scott M.; Calla, Bernarda] ARS, USDA, Daniel K Inouye US Pacific Basin Agr Res Ctr, Trop Crop & Commod Protect Res Unit, Hilo, HI 96720 USA. [Mitchell, Robert F.; Bledsoe, Tiffany] Univ Arizona, Ctr Insect Sci, Tucson, AZ 85721 USA. [Mitchell, Robert F.; Bledsoe, Tiffany] Univ Arizona, Dept Neurosci, Tucson, AZ 85721 USA. [Mitchell, Robert F.] Univ Wisconsin, Dept Biol, Oshkosh, WI 54901 USA. [Waterhouse, Robert M.; Ioannidis, Panagiotis; Zdobnov, Evgeny M.] Univ Geneva, Dept Genet Med & Dev, CH-1211 Geneva, Switzerland. [Waterhouse, Robert M.; Ioannidis, Panagiotis; Zdobnov, Evgeny M.] Univ Geneva, Swiss Inst Bioinformat, CH-1211 Geneva, Switzerland. [Waterhouse, Robert M.] MIT, Cambridge, MA 02142 USA. [Waterhouse, Robert M.] Broad Inst MIT & Harvard, Cambridge, MA 02142 USA. [Arsala, Deanna; Cohen, Lorna; Lynch, Jeremy A.; Pers, Dan; Quan, Honghu] Univ Illinois, Dept Biol Sci, Chicago, IL 60607 USA. [Benoit, Joshua B.; Rosendale, Andrew J.] Univ Cincinnati, Dept Biol Sci, Cincinnati, OH 45221 USA. [Blackmon, Heath; Demuth, Jeffery P.] Univ Texas Arlington, Dept Biol, Arlington, TX 76019 USA. [Bowsher, Julia H.; Torson, Alex S.] North Dakota State Univ, Dept Biol Sci, Fargo, ND 58108 USA. [Chao, Hsu; Dinh, Huyen; Doddapaneni, HarshaVardhan; Dugan, Shannon; Han, Yi; Hughes, Daniel S. T.; Lee, Sandra L.; Murali, Shwetha C.; Muzny, Donna M.; Qu, Jiaxin; Worley, Kim C.; Gibbs, Richard A.; Richards, Stephen] Baylor Coll Med, Dept Human & Mol Genet, Human Genome Sequencing Ctr, One Baylor Plaza, Houston, TX 77030 USA. [Childers, Anna K.; Rinehart, Joseph P.; Roehrdanz, Richard; Yocum, George] ARS, USDA, Red River Valley Agr Res Ctr, Biosci Res Lab, Fargo, ND 58102 USA. [Childers, Christopher; Lee, Chien-Yueh; Lin, Han; Poelchau, Monica F.] ARS, USDA, Natl Agr Lib, Beltsville, MD 20705 USA. [Dolan, Amanda; Silva, Christian; Werren, John H.] Univ Rochester, Dept Biol, Rochester, NY 14627 USA. [Duan, Jian J.] ARS, USDA, Beneficial Insects Intro Res, Newark, DE 19713 USA. [Friedrich, Markus; Jones, Jeffery W.] Wayne State Univ, Dept Biol Sci, Detroit, MI 48202 USA. [Glastad, Karl M.; Goodisman, Michael A. D.] Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA. [Johnston, J. Spencer] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. [Kuhn, Leslie A.] Michigan State Univ, Dept Biochem & Mol Biol, Dept Comp Sci & Engn, E Lansing, MI 48824 USA. [Kuhn, Leslie A.] Michigan State Univ, Dept Fisheries & Wildlife, E Lansing, MI 48824 USA. [Lance, David R.] US Anim & Plant Hlth Inspect Serv, USDA, Plant Pest & Quarantine, Ctr Plant Hlth Sci & Technol,Otis Lab, Buzzards Bay, MA 02542 USA. [Lee, Chien-Yueh; Lin, Han] Natl Taiwan Univ, Grad Inst Biomed Elect & Bioinformat, Taipei 10617, Taiwan. [Moczek, Armin P.] Indiana Univ, Dept Biol, Blomington, IN 47405 USA. [Nelson, David R.] Univ Tennessee, Ctr Hlth Sci, Dept Microbiol Immunol & Biochem, Memphis, TN 38163 USA. [Palli, Subba R.] Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA. [Panfilio, Kristen A.; Jentzsch, Iris M. Vargas] Univ Cologne, Inst Dev Biol, D-50674 Cologne, Germany. [Ray, Ann M.] Xavier Univ, Dept Biol, Cincinnati, OH 45207 USA. [Robertson, Hugh M.] Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. RP McKenna, DD (reprint author), Univ Memphis, Dept Biol Sci, 3700 Walker Ave, Memphis, TN 38152 USA.; Richards, S (reprint author), Baylor Coll Med, Dept Human & Mol Genet, Human Genome Sequencing Ctr, One Baylor Plaza, Houston, TX 77030 USA. EM dmckenna@memphis.edu; stephenr@bcm.edu RI Zdobnov, Evgeny/K-1133-2012; OI Blackmon, Heath/0000-0002-5433-4036; Waterhouse, Robert/0000-0003-4199-9052; Childers, Anna/0000-0002-0747-8539 FU NHGRI [U54 HG003273]; University of Memphis FedEx Institute; US NSF [DEB1355169]; USDA-APHIS cooperative agreement [15-8130-0547-CA]; USDA NIFA [2015-67013-23287]; NSF [IOS1120209, IOS1256689, DEB1257053]; University of Cincinnati Faculty Development Research grant; Marie Curie International Outgoing Fellowship [PIOF-GA-2011-303312]; Swiss NSF awards [31003A-125350, 31003A-143936]; Max-Planck-Gesellschaft; German Research Foundation (DFG) [PA 2044/1-1, SFB 680]; NIH postdoctoral training grant [5 K12 GM000708-15] FX Funding for genome sequencing, assembly and automated annotation was provided by NHGRI grant U54 HG003273 to RAG. The i5k pilot project (https://www.hgsc.bcm.edu/arthropods) assisted in sequencing of the A. glabripennis, A. planipennis, and O. taurus genomes. We acknowledge funding from the University of Memphis FedEx Institute, US NSF grant DEB1355169 and USDA-APHIS cooperative agreement 15-8130-0547-CA to DDM, USDA NIFA grant 2015-67013-23287 to KH and ES, NSF grant DEB1257053 to JHW, NSF grants IOS1120209 and IOS1256689 to APM, University of Cincinnati Faculty Development Research grant to JBB, Marie Curie International Outgoing Fellowship PIOF-GA-2011-303312 to RMW, Swiss NSF awards 31003A-125350 and 31003A-143936 to EMZ, Max-Planck-Gesellschaft to YP, RK and AB, German Research Foundation (DFG) grants PA 2044/1-1 and SFB 680 to KAP, and NIH postdoctoral training grant 5 K12 GM000708-15 to RFM. NR 81 TC 3 Z9 3 U1 17 U2 17 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1474-760X J9 GENOME BIOL JI Genome Biol. PD NOV 11 PY 2016 VL 17 AR 227 DI 10.1186/s13059-016-1088-8 PG 18 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA EE1WF UT WOS:000389373800001 PM 27832824 ER PT J AU Silva, MG Knowles, DP Suarez, CE AF Silva, Marta G. Knowles, Donald P. Suarez, Carlos E. TI Identification of interchangeable cross-species function of elongation factor-1 alpha promoters in Babesia bigemina and Babesia bovis SO PARASITES & VECTORS LA English DT Article DE Babesia bovis; Babesia bigemina; Transfection; Elongation factor-1 alpha ID GENE-EXPRESSION; TRANSMISSION; CULTIVATION; INFECTION; GENOME AB Background: Tick-borne Babesia bigemina is responsible for acute and potentially lethal hemolytic disease in cattle. The development of genetic manipulation tools necessary to the better understanding of parasite biology is currently limited by the lack of a complete parasite genome and experimental tools such as transfection. Effective promoters, required to regulate expression of transgenes, such as the elongation factor-1 alpha (ef-1 alpha), have been identified in other apicomplexans such as Babesia bovis and Plasmodium falciparum. Methods: The B. bigemina ef-1 alpha locus was defined by searching a partial genome library of B. bigemina (Sanger Institute). Presence of an intron in the 5' untranslated region was determined by 5' Rapid Amplification of cDNA Ends (RACE) analysis. Promoter activity was determined by measurement of luciferase expression at several time points after electroporation, efficiency of transfections and normalization of data was determined by quantitative PCR and by the percentage of parasitized erythrocytes. Results: The ef-1 alpha locus contains two identical head to head ef-1 alpha genes separated by a 1.425 kb intergenic (IG) region. Significant sequence divergence in the regions upstream of the inverted repeats on each side of the B. bigemina IG region suggest independent regulation mechanisms for controlling expression of each of the two ef-1 alpha genes. Plasmid constructs containing the 5' and 3' halves of the IG regions controlling the expression of the luciferase gene containing a 3' region of a B. bigemina rap-1 alpha gene, were generated for the testing of luciferase activity in transiently transfected parasites. Both halves of the ef-1 alpha IG region tested showed the ability to promote high level production of luciferase. Moreover, both B. bigemina ef-1 alpha promoters are also active in transiently transfected B. bovis and conversely, a B. bovis ef-1 alpha promoter is active in transiently transfected B. bigemina. Conclusions: Collectively these data demonstrate the existence of two distinct promoters with homologous and heterologous promoter function in B. bigemina and B. bovis which is described for the first time in Babesia species. This study is of significance for development of interspecies stable transfection systems for B. bigemina and for B. bovis. C1 [Silva, Marta G.; Knowles, Donald P.; Suarez, Carlos E.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Knowles, Donald P.; Suarez, Carlos E.] ARS, Anim Dis Res Unit, USDA, WSU, Pullman, WA USA. RP Silva, MG (reprint author), Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. EM marta@vetmed.wsu.edu FU United States Department of Agriculture-Agriculture Research Service Current Research Information System [5348-32000-028-00D]; BARD Project [US-4700-14] FX This work was supported by United States Department of Agriculture-Agriculture Research Service Current Research Information System Project No. 5348-32000-028-00D and BARD Project number US-4700-14. The funders had no role in study design, data analysis, or preparation and decision to publish the manuscript. NR 21 TC 0 Z9 0 U1 1 U2 1 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD NOV 11 PY 2016 VL 9 AR 576 DI 10.1186/s13071-016-1859-9 PG 9 WC Parasitology SC Parasitology GA EC5AM UT WOS:000388145500001 PM 27835993 ER PT J AU Ikezaki, H Ai, M Schaefer, EJ Otokozawa, S Asztalos, BF Nakajima, K Zhou, YH Liu, CT Jacques, PF Cupples, LA Furusyo, N AF Ikezaki, Hiroaki Ai, Masumi Schaefer, Ernst J. Otokozawa, Seiko Asztalos, Bela F. Nakajima, Katsuyuki Zhou, Yanhua Liu, Ching-Ti Jacques, Paul F. Cupples, L. Adrienne Furusyo, Norihiro TI Ethnic Differences in Glucose Homeostasis Markers between the Kyushu-Okinawa Population Study and the Framingham Offspring Study SO SCIENTIFIC REPORTS LA English DT Article ID BETA-CELL FUNCTION; CORONARY-HEART-DISEASE; JAPANESE POPULATION; INSULIN SENSITIVITY; DIABETES-MELLITUS; GLYCATED ALBUMIN; MEN; ADIPONECTIN; OBESITY; ASSOCIATION AB We compared markers of glucose homeostasis and their association with diabetes and impaired fasting glucose (IFG) in Fukuoka, Japanese subjects (n = 1108) and age-, gender- and menopausal status-matched participants in the Framingham Offspring Study (n = 1096). The markers examined included fasting glucose, insulin, adiponectin, and glycated albumin, as well as body mass index (BMI), use of medications, and history of diabetes. The results showed that IFG prevalence in Japanese men (15.9%) and women (7.4%) were 50% less than those observed in Framingham men (34.5%) and women (21.4%) (P < 0.001). However, the diabetes prevalence in Japanese men at 13.3% was twice as high (P < 0.01) as the rate in Framingham men at 6.5%, while these rates were similar in women. Median insulin levels in Japanese men (4.6 mu IU/mL) and women (4.3 mu IU/mL) were about 50% lower (P < 0.001) than those in Framingham men (10.8 mu IU/mL) and women (9.9 mu IU/mL), as were insulin resistance values (P < 0.001). These population differences were also observed after subjects were stratified by glucose levels. In conclusion, our data indicate that there is significantly less IFG, lower insulin levels, and insulin resistance, but higher diabetes prevalence in Fukuoka men than in Framingham men, indicating that insulin deficiency may be an important cause of diabetes in Japan. C1 [Ikezaki, Hiroaki; Schaefer, Ernst J.; Asztalos, Bela F.] Tufts Univ, Cardiovasc Nutr Lab, Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. [Ikezaki, Hiroaki; Schaefer, Ernst J.; Asztalos, Bela F.; Jacques, Paul F.] Tufts Univ, Sch Med, 711 Washington St, Boston, MA 02111 USA. [Ikezaki, Hiroaki; Jacques, Paul F.] Tufts Univ, Human Nutr Res Ctr Aging, Nutr Epidemiol Program, 711 Washington St, Boston, MA 02111 USA. [Ikezaki, Hiroaki; Furusyo, Norihiro] Kyushu Univ Hosp, Dept Gen Internal Med, Higashi Ku, 3-1-1 Maidashi, Fukuoka 8128582, Japan. [Ai, Masumi] Tokyo Med & Dent Univ Hosp, Dept Insured Med Care Management, Bunkyo Ku, 1-5-45 Yushima, Tokyo 1138510, Japan. [Otokozawa, Seiko] Sapporo Med Univ, Dept Publ Hlth, Sch Med, Chuo Ku, S1 W17, Sapporo, Hokkaido 0608556, Japan. [Nakajima, Katsuyuki] Gunma Univ, Dept Clin Lab Med, 3-39-22 Showa Machi, Maebashi, Gunma 3718511, Japan. [Zhou, Yanhua; Liu, Ching-Ti; Cupples, L. Adrienne] Boston Univ, Sch Publ Hlth, Dept Biostat, 801 Massachusetts Ave CT3, Boston, MA 02118 USA. RP Schaefer, EJ (reprint author), Tufts Univ, Cardiovasc Nutr Lab, Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA.; Schaefer, EJ (reprint author), Tufts Univ, Sch Med, 711 Washington St, Boston, MA 02111 USA. EM Ernst.Schaefer@tufts.edu FU Japan Heart Foundation/Bayer Yakuhin Research Grant Abroad Program, Tokyo, Japan; Japan Multi-institutional Collaborative Cohort Study (J-MICC Study); Scientific Support Programs for Cancer Research [221S0001]; 21st Century COE Program [17015018]; Ministry of Education, Culture, Sports Science and Technology of Japan [26461506]; US. Department of Agriculture - Agricultural Research Service [58-1950-0-014, 58-1950-4-003]; National Institutes of Health [P50 HL083813-01]; Otsuka Pharmaceutical Corporation, Tokyo, Japan; NHLBI from the National Institute of Health [N01-HC 25195] FX The authors thank Katalin V. Horvath for her excellent technical assistance. H.I. was supported by the Japan Heart Foundation/Bayer Yakuhin Research Grant Abroad Program, Tokyo, Japan. Research on the Kyushu-Okinawa Population Study and N.F. were supported by the Japan Multi-institutional Collaborative Cohort Study (J-MICC Study), a Scientific Support Programs for Cancer Research Grant-in-Aid for Scientific Research on Innovative Areas (grant number 221S0001), a Grant-in-Aid for Comprehensive Research (Priority Areas of Cancer: grant number 17015018) of the 21st Century COE Program, and partially supported Grant-in-Aid for Scientific Research (C), 2014-2016 (grant number 26461506) from the Ministry of Education, Culture, Sports Science and Technology of Japan. E.J.S., B.F.A. and P.F.J. were supported by the US. Department of Agriculture - Agricultural Research Service under Specific Cooperative Agreements #58-1950-0-014 and #58-1950-4-003, and Project Grant P50 HL083813-01 from the National Institutes of Health. Assay kits for glycated albumin were provided by Asahi-Kasei Corporation, Tokyo, Japan, and kits for adiponectin and insulin were provided by the Otsuka Pharmaceutical Corporation, Tokyo, Japan, and these companies also provided fellowship support for M.A. and A.O. Support for the Framingham Offspring Study and L.A.C., C.L. and Z.Y. were provided by grant NHLBI N01-HC 25195 from the National Institute of Health. The contents of this article are solely the responsibility of the authors and do not necessarily represent the official views of any other organization including Kyushu University, Tufts University, Boston University, the US. Department of Agriculture, or the National Institutes of Health. NR 31 TC 0 Z9 0 U1 1 U2 1 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD NOV 10 PY 2016 VL 6 AR 36725 DI 10.1038/srep36725 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EC4GY UT WOS:000388086400001 PM 27830830 ER PT J AU Douma, JC Pautasso, M Venette, RC Robinet, C Hemerik, L Mourits, MCM Schans, J van der Werf, W AF Douma, J. C. Pautasso, M. Venette, R. C. Robinet, C. Hemerik, L. Mourits, M. C. M. Schans, J. van der Werf, W. TI Pathway models for analysing and managing the introduction of alien plant pests-an overview and categorization SO ECOLOGICAL MODELLING LA English DT Review DE Agent-based modelling; Alien species; Gravity models; Epidemic network models; Plant disease; Risk assessment ID HUMAN-MEDIATED DISPERSAL; ECONOMIC-IMPACT; TRADE; RISK; SPREAD; ESTABLISHMENT; INVASIONS; NETWORKS; BIOSECURITY; COLEOPTERA AB Alien plant pests are introduced into new areas at unprecedented rates through global trade, transport, tourism and travel, threatening biodiversity and agriculture. Increasingly, the movement and introduction of pests is analysed with pathway models to provide risk managers with quantitative estimates of introduction risks and effectiveness of management options. Pathway models vary greatly in mathematical form, level of detail, treatment of uncertainty and variability, as well as terminology. We conducted an overview and cluster analysis of pathway models to guide risk assessors, risk managers and model developers. We performed divisive hierarchical clustering on models retrieved from the peer-reviewed and grey literature to characterise and categorize the currently used modelling approaches. We distinguish two clusters of models based on product volume flows and two clusters of models based on the movement of individual agents. The first cluster of flow-based models describes a flow of infested material from origins to destinations according to fixed partitioning coefficients. These deterministic models can account for consequences of parameter variability and uncertainty. The second and third clusters of pathway models incorporate stochasticity in processes, and are respectively flow-based or agent-based. Models in the fourth cluster account for interactions between agents and nodes in the pathway. Conceptually, there are no fundamental differences between epidemic network models and this last cluster of pathway models. The choice of pathway model depends on the aim of the risk assessment, the available time, expertise and data. Models in clusters 2, 3, and 4 add sophistication and insight in variability to pathway analysis, but under time and data constraints, key objectives of risk assessors and managers can be addressed with models in cluster 1. The four clusters represent a comprehensive and fit for purpose toolbox of models. (C) 2016 Elsevier B.V. All rights reserved. C1 [Douma, J. C.; van der Werf, W.] Wageningen Univ, Ctr Crop Syst Anal, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands. [Pautasso, M.] European Food Safety Author, Anim & Plant Hlth Unit, Via Carlo Magno 1A, I-43126 Parma, Italy. [Venette, R. C.] US Forest Serv, Northern Res Stn, USDA, 1561 Lindig St, St Paul, MN 55117 USA. [Robinet, C.] INRA, Zool Forestiere UR633, F-45075 Orleans, France. [Hemerik, L.] Wageningen Univ, Biometris, Dept Math & Stat Methods, POB 16, NL-6700 AA Wageningen, Netherlands. [Mourits, M. C. M.] Wageningen Univ, Business Econ Grp, Hollandseweg 1, NL-6706 KN Wageningen, Netherlands. [Schans, J.] Netherlands Food & Consumer Prod Safety Author, POB 43006, NL-3540 AA Utrecht, Netherlands. RP Douma, JC (reprint author), Wageningen Univ, Ctr Crop Syst Anal, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands. EM bob.douma@wur.nl; marco.pautasso@efsa.europa.eu; rvenette@fs.fed.us; christelle.robinet@orleans.inra.fr; lia.hemerik@wur.nl; monique.mourits@wur.nl; j.schans@nvwa.nl; wopke.vanderwerf@wur.nl FU European Food Safety Authority (EFSA) [CT/EFSA/PLH/2011/04] FX This work was financially supported by the European Food Safety Authority (EFSA) under Service contract CT/EFSA/PLH/2011/04: Development of probabilistic models for quantitative pathway analysis of plant pests' introduction for the EU territory (lot 2). The positions and opinions presented in this article are those of the authors alone and are not intended to represent the views or scientific works of EFSA. We thank Katharina Dehnen-Schmutz, Karen Garrett, Tom Harwood, Ottmar Holdenrieder, Mike Jeger, Olaf Mosbach-Schultz, Mathieu Moslonka-Lefebvre, Tomasz Oszako, Francoise Petter, Mike Shaw, Giuseppe Stancanelli and Muriel Suffert for discussions, insights and support. NR 73 TC 0 Z9 0 U1 13 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3800 EI 1872-7026 J9 ECOL MODEL JI Ecol. Model. PD NOV 10 PY 2016 VL 339 SI SI BP 58 EP 67 DI 10.1016/j.ecolmode1.2016.08.009 PG 10 WC Ecology SC Environmental Sciences & Ecology GA DY1RB UT WOS:000384871000006 ER PT J AU Fanta, GF Felker, FC Selling, GW Hay, WT Biswas, A AF Fanta, George F. Felker, Frederick C. Selling, Gordon W. Hay, William T. Biswas, Atanu TI Poly(vinyl alcohol) composite films with high percent elongation prepared from amylose-fatty ammonium salt inclusion complexes SO JOURNAL OF APPLIED POLYMER SCIENCE LA English DT Article DE blends; compatibilization; hydrophilic polymers; polysaccharides; properties and characterization ID STARCH/PVA BLEND FILMS; POLYVINYL-ALCOHOL; CHAIN-LENGTH; AMYLOPECTIN; RETROGRADATION; GELATINIZATION; ADDITIVES; GELATION AB Amylose inclusion complexes prepared from cationic fatty ammonium salts and jet-cooked high amylose starch were combined with poly(vinyl alcohol) (PVOH) to form glycerol-plasticized films. For the octadecylammonium salt complexes, elongation was significantly higher than the PVOH control when the amount of complex incorporated was from 20% to 70%. For the dodecyl- and hexadecylammonium salt complexes, elongation was significantly higher than PVOH films for 20% to 40% incorporation of cationic complex. Tensile strength declined with increasing levels of amylose-ammonium salt complex, and surface hydrophobicity (contact angle) was significantly higher than PVOH films. Microscopy showed no phase separation or phase inversion, suggesting intimate mixing due to ionic interactions between cationic ammonium salt complexes and the hydroxyl groups of PVOH. The high elongations of these films and increased water contact angles are marketable advantages, along with the lower cost and increased biodegradability of the starch-based component. (c) 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 44110. C1 [Fanta, George F.; Selling, Gordon W.; Hay, William T.; Biswas, Atanu] ARS, USDA, Natl Ctr Agr Utilizat Res, Plant Polymer Res, Peoria, IL USA. [Felker, Frederick C.] ARS, USDA, Natl Ctr Agr Utilizat Res, Funct Foods Res, Peoria, IL 61604 USA. RP Felker, FC (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, Funct Foods Res, Peoria, IL 61604 USA. EM frederick.felker@ars.usda.gov NR 31 TC 0 Z9 0 U1 22 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8995 EI 1097-4628 J9 J APPL POLYM SCI JI J. Appl. Polym. Sci. PD NOV 10 PY 2016 VL 133 IS 42 AR 44110 DI 10.1002/app.44110 PG 8 WC Polymer Science SC Polymer Science GA DS4AM UT WOS:000380723800010 ER PT J AU Irynayatsiuk Irjasaar Kalamees, K Sulaymonov, S Gafforov, Y O'Donnell, K AF Irynayatsiuk Irjasaar Kalamees, Kuulo Sulaymonov, Shaxob Gafforov, Yusufjon O'Donnell, Kerry TI Epitypification of Morchella steppicola (Morchellaceae, Pezizales), a morphologically, phylogenetically and biogeographically distinct member of the Esculenta Clade from central Eurasia SO Phytotaxa LA English DT Article DE Central Asia; conservation; ITS rDNA; molecular phylogenetics; steppe morel; Ukraine ID TRUE MORELS MORCHELLA; REVEALS AB The steppe morel, Morchella steppicola, is one of the more iconic species of true morels (Morchellaceae, Pezizales) based on its: 1) distinctive cerebriform pileus with densely packed labyrinthine irregular ridges, 2) genealogically exclusive position as the earliest diverging species lineage within the Esculenta clade, and 3) geographic distribution within temperate grassland steppes in central Eurasia. Given the uniqueness of this species, and conservation efforts in some Eurasian countries to protect it, we sought to study the holotype in the Mycological Herbarium of the M. G. Kholodny Institute of Botany (KW), Kyiv, Ukraine. However, because the type specimen appears to have been lost, but a picture was provided with the description, we have designated it the lectotype and epitypified this important species based on a collection made in 2014 from Lugansk province, Ukraine. Herein, we provide a detailed morphological description, provide a preliminary assessment of intraspecific diversity via phylogenetic analysis of ITS rDNA sequences from 13 M. steppicola collections spanning six Eurasian countries, and map its geographic distribution across the terrestrial ecoregions of central Eurasia. C1 [Irynayatsiuk] Kharkov Natl Univ, Dept Mycol & Plant Resistance, Maidan Svobody 4, UA-61022 Kharkov, Ukraine. [Irjasaar; Kalamees, Kuulo] Univ Tartu, Inst Ecol & Environm Sci, Ravila St 14A, EE-50411 Tartu, Estonia. [Kalamees, Kuulo] Estonian Univ Life Sci, Inst Agr & Environm Sci, Fr R Kreutzwaldi St 5D, EE-51014 Tartu, Estonia. [Sulaymonov, Shaxob] Navoi State Pedag Inst, 3 Janibuy St, Navoi, Uzbekistan. [Gafforov, Yusufjon] Acad Sci Uzbek, Inst Gene Pool Plants & Anim, Lab Mycol, 232 Bogishamol St, Tashkent 10053, Uzbekistan. [O'Donnell, Kerry] USDA ARS, Natl Ctr Agr Utilizat Res, Mycotoxin Prevent & Appl Microbiol Res Unit, Peoria, IL 60604 USA. RP Irynayatsiuk (reprint author), Kharkov Natl Univ, Dept Mycol & Plant Resistance, Maidan Svobody 4, UA-61022 Kharkov, Ukraine. EM irina_disco@yahoo.com FU Estonian Ministry of Education and Research [IUT20-30]; European Regional Development Fund (Centre of Excellence EcolChange) FX We thank the directors and curators of the CWU, KW and TAAM fungaria for loan of specimens and Alevtina Ponomareva for collecting specimens and providing photos of fresh specimens. The authors are indebted to Prof I.O. Dudka and M.O. Zykova for assistance with specimens from the KW herbarium. Thanks are due Drs. Scott Redhead and Keith A. Seifert, Agriculture and Agri-Food Canada, Ottawa, for nomenclatural advice concerning the lecto- and epitypification. IS was supported by the institutional research funding IUT20-30 of the Estonian Ministry of Education and Research and by the European Regional Development Fund (Centre of Excellence EcolChange). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 45 TC 0 Z9 0 U1 4 U2 4 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1179-3155 EI 1179-3163 J9 PHYTOTAXA JI Phytotaxa PD NOV 9 PY 2016 VL 284 IS 1 BP 31 EP 40 DI 10.11646/phytotaxa.284.1.3 PG 10 WC Plant Sciences SC Plant Sciences GA EE0VK UT WOS:000389297300003 ER PT J AU Cherubin, P Garcia, MC Curtis, D Britt, CBT Craft, JW Burress, H Berndt, C Reddy, S Guyette, J Zheng, TY Huo, Q Quinones, B Briggs, JM Teter, K AF Cherubin, Patrick Garcia, Maria Camila Curtis, David Britt, Christopher B. T. Craft, John W., Jr. Burress, Helen Berndt, Chris Reddy, Srikar Guyette, Jessica Zheng, Tianyu Huo, Qun Quinones, Beatriz Briggs, James M. Teter, Ken TI Inhibition of Cholera Toxin and Other AB Toxins by Polyphenolic Compounds SO PLOS ONE LA English DT Article ID ENTEROHEMORRHAGIC ESCHERICHIA-COLI; INDUCED FLUID ACCUMULATION; ENDOPLASMIC-RETICULUM; SHIGA TOXIN; ADP-RIBOSYLTRANSFERASE; DEGRADATION PATHWAY; PLANT POLYPHENOLS; A1 POLYPEPTIDE; TARGET-CELLS; VERO CELLS AB Cholera toxin (CT) is an AB-type protein toxin that contains a catalytic A1 subunit, an A2 linker, and a cell-binding B homopentamer. The CT holotoxin is released into the extracellular environment, but CTA1 attacks a target within the cytosol of a host cell. We recently reported that grape extract confers substantial resistance to CT. Here, we used a cell culture system to identify twelve individual phenolic compounds from grape extract that inhibit CT. Additional studies determined the mechanism of inhibition for a subset of the compounds: two inhibited CT binding to the cell surface and even stripped CT from the plasma membrane of a target cell; two inhibited the enzymatic activity of CTA1; and four blocked cytosolic toxin activity without directly affecting the enzymatic function of CTA1. Individual polyphenolic compounds from grape extract could also generate cellular resistance to diphtheria toxin, exotoxin A, and ricin. We have thus identified individual toxin inhibitors from grape extract and some of their mechanisms of inhibition against CT. C1 [Cherubin, Patrick; Garcia, Maria Camila; Curtis, David; Britt, Christopher B. T.; Burress, Helen; Berndt, Chris; Reddy, Srikar; Guyette, Jessica; Teter, Ken] Univ Cent Florida, Coll Med, Burnett Sch Biomed Sci, Orlando, FL 32816 USA. [Craft, John W., Jr.; Briggs, James M.] Univ Houston, Dept Biol & Biochem, Houston, TX USA. [Zheng, Tianyu; Huo, Qun] Univ Cent Florida, NanoSci Technol Ctr, Orlando, FL 32816 USA. [Zheng, Tianyu; Huo, Qun] Univ Cent Florida, Dept Chem, Orlando, FL 32816 USA. [Quinones, Beatriz] USDA ARS, Produce Safety & Microbiol Res Unit, Western Reg Res Ctr, Albany, CA USA. [Curtis, David] Valencia Coll, Orlando, FL USA. RP Teter, K (reprint author), Univ Cent Florida, Coll Med, Burnett Sch Biomed Sci, Orlando, FL 32816 USA. EM kteter@mail.ucf.edu FU U.S. Department of Agriculture, Agricultural Research Service [2030-42000-051-00D, 58-5325-4-024] FX This material is based upon work supported by the U.S. Department of Agriculture, Agricultural Research Service (www.ars.usda.gov/)-CRIS project no. 2030-42000-051-00D under the Non-Assistance Cooperative Agreement (NACA) no. 58-5325-4-024 to KT. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 45 TC 0 Z9 0 U1 3 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 9 PY 2016 VL 11 IS 11 AR e0166477 DI 10.1371/journal.pone.0166477 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EB9OG UT WOS:000387724300110 PM 27829022 ER PT J AU Young, MK Isaak, DJ McKelvey, KS Wilcox, TM Bingham, DM Pilgrim, KL Carim, KJ Campbell, MR Corsi, MP Horan, DL Nagel, DE Schwartz, MK AF Young, Michael K. Isaak, Daniel J. McKelvey, Kevin S. Wilcox, Taylor M. Bingham, Daniel M. Pilgrim, Kristine L. Carim, Kellie J. Campbell, Matthew R. Corsi, Matthew P. Horan, Dona L. Nagel, David E. Schwartz, Michael K. TI Climate, Demography, and Zoogeography Predict Introgression Thresholds in Salmonid Hybrid Zones in Rocky Mountain Streams SO PLOS ONE LA English DT Article ID WESTSLOPE CUTTHROAT TROUT; INTRODUCED RAINBOW-TROUT; ONCORHYNCHUS-CLARKII-LEWISI; WHITEFISH COREGONUS SP.; O-MYKISS; BIOLOGICAL INVASIONS; BRITISH-COLUMBIA; STEELHEAD TROUT; NONNATIVE TROUT; TRADE-OFFS AB Among the many threats posed by invasions of nonnative species is introgressive hybridization, which can lead to the genomic extinction of native taxa. This phenomenon is regarded as common and perhaps inevitable among native cutthroat trout and introduced rainbow trout in western North America, despite that these taxa naturally co-occur in some locations. We conducted a synthetic analysis of 13,315 genotyped fish from 558 sites by building logistic regression models using data from geospatial stream databases and from 12 published studies of hybridization to assess whether environmental covariates could explain levels of introgression between westslope cutthroat trout and rainbow trout in the U. S. northern Rocky Mountains. A consensus model performed well (AUC, 0.78-0.86; classification success, 72-82%; 10-fold cross validation, 70-82%) and predicted that rainbow trout introgression was significantly associated with warmer water temperatures, larger streams, proximity to warmer habitats and to recent sources of rainbow trout propagules, presence within the historical range of rainbow trout, and locations further east. Assuming that water temperatures will continue to rise in response to climate change and that levels of introgression outside the historical range of rainbow trout will equilibrate with those inside that range, we applied six scenarios across a 55,234-km stream network that forecast 9.574.7% declines in the amount of habitat occupied by westslope cutthroat trout populations of conservation value, but not the wholesale loss of such populations. We conclude that introgression between these taxa is predictably related to environmental conditions, many of which can be manipulated to foster largely genetically intact populations of westslope cutthroat trout and help managers prioritize conservation activities. C1 [Young, Michael K.; McKelvey, Kevin S.; Wilcox, Taylor M.; Bingham, Daniel M.; Pilgrim, Kristine L.; Carim, Kellie J.; Schwartz, Michael K.] US Forest Serv, Natl Genom Ctr Wildlife & Fish Conservat, Rocky Mt Res Stn, USDA, Missoula, MT 59812 USA. [Isaak, Daniel J.; Horan, Dona L.; Nagel, David E.] US Forest Serv, Rocky Mt Res Stn, Boise, ID USA. [Wilcox, Taylor M.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Campbell, Matthew R.] Idaho Dept Fish & Game, Eagle Fish Genet Lab, Eagle, ID USA. [Corsi, Matthew P.] Idaho Dept Fish & Game, Coeur Dalene, ID USA. [Bingham, Daniel M.] Rogue Biol Consultants, Vancouver, WA USA. RP Young, MK (reprint author), US Forest Serv, Natl Genom Ctr Wildlife & Fish Conservat, Rocky Mt Res Stn, USDA, Missoula, MT 59812 USA. EM mkyoung@fs.fed.us FU Region 1 of the U.S. Forest Service; Rocky Mountain Research Station; U.S. Forest Service Washington Office FX Funding for this work was provided by Region 1 of the U.S. Forest Service. The funder provided support in the form of salaries for authors [DMB], but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the 'author contributions' section.; Funding for this work was provided by Region 1 of the U.S. Forest Service. Institutional support was provided by the National Genomics Center for Wildlife and Fish Conservation, supported by the Rocky Mountain Research Station and U.S. Forest Service Washington Office. NR 100 TC 1 Z9 1 U1 14 U2 14 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 9 PY 2016 VL 11 IS 11 AR e0163563 DI 10.1371/journal.pone.0163563 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EB9OG UT WOS:000387724300007 PM 27828980 ER PT J AU Islam, MS Thyssen, GN Jenkins, JN Zeng, LH Delhom, CD McCarty, JC Deng, DD Hinchliffe, DJ Jones, DC Fang, DD AF Islam, Md Sariful Thyssen, Gregory N. Jenkins, Johnie N. Zeng, Linghe Delhom, Christopher D. McCarty, Jack C. Deng, Dewayne D. Hinchliffe, Doug J. Jones, Don C. Fang, David D. TI A MAGIC population-based genome-wide association study reveals functional association of GhRBB1_A07 gene with superior fiber quality in cotton SO BMC GENOMICS LA English DT Article DE Cotton; Fiber quality; Genome wide association study; Genotyping-by-sequencing; Multi parent advanced generation inter-cross ID GOSSYPIUM-HIRSUTUM L.; QUANTITATIVE TRAIT LOCI; LINKAGE DISEQUILIBRIUM; QTL ANALYSIS; ZEA-MAYS; GERMPLASM; RESISTANCE; CULTIVARS; LINES; MAIZE AB Background: Cotton supplies a great majority of natural fiber for the global textile industry. The negative correlation between yield and fiber quality has hindered breeders' ability to improve these traits simultaneously. A multi-parent advanced generation inter-cross (MAGIC) population developed through random-mating of multiple diverse parents has the ability to break this negative correlation. Genotyping-by-sequencing (GBS) is a method that can rapidly identify and genotype a large number of single nucleotide polymorphisms (SNP). Genotyping a MAGIC population using GBS technologies will enable us to identify marker-trait associations with high resolution. Results: An Upland cotton MAGIC population was developed through random-mating of 11 diverse cultivars for five generations. In this study, fiber quality data obtained from four environments and 6071 SNP markers generated via GBS and 223 microsatellite markers of 547 recombinant inbred lines (RILs) of the MAGIC population were used to conduct a genome wide association study (GWAS). By employing a mixed linear model, GWAS enabled us to identify markers significantly associated with fiber quantitative trait loci (QTL). We identified and validated one QTL cluster associated with four fiber quality traits [short fiber content (SFC), strength (STR), length (UHM) and uniformity (UI)] on chromosome A07. We further identified candidate genes related to fiber quality attributes in this region. Gene expression and amino acid substitution analysis suggested that a regeneration of bulb biogenesis 1 (GhRBB1_A07) gene is a candidate for superior fiber quality in Upland cotton. The DNA marker CFBid0004 designed from an 18 bp deletion in the coding sequence of GhRBB1_A07 in Acala Ultima is associated with the improved fiber quality in the MAGIC RILs and 105 additional commercial Upland cotton cultivars. Conclusion: Using GBS and a MAGIC population enabled more precise fiber QTL mapping in Upland cotton. The fiber QTL and associated markers identified in this study can be used to improve fiber quality through marker assisted selection or genomic selection in a cotton breeding program. Target manipulation of the GhRBB1_A07 gene through biotechnology or gene editing may potentially improve cotton fiber quality. C1 [Islam, Md Sariful; Fang, David D.] USDA ARS, Cotton Fiber Biosci Res Unit, Southern Reg Res Ctr, New Orleans, LA 70124 USA. [Thyssen, Gregory N.; Hinchliffe, Doug J.] USDA ARS, Cotton Chem & Utilizat Res Unit, Southern Reg Res Ctr, New Orleans, LA 70124 USA. [Jenkins, Johnie N.; McCarty, Jack C.; Deng, Dewayne D.] USDA ARS, Genet & Sustainable Agr Res Unit, Mississippi State, MS 39762 USA. [Zeng, Linghe] USDA ARS, Crop Genet Res Unit, Stoneville, MS 38772 USA. [Delhom, Christopher D.] USDA ARS, Cotton Struct & Qual Res Unit, Southern Reg Res Ctr, New Orleans, LA 70124 USA. [Jones, Don C.] Cotton Inc, Cary, NC 27513 USA. RP Fang, DD (reprint author), USDA ARS, Cotton Fiber Biosci Res Unit, Southern Reg Res Ctr, New Orleans, LA 70124 USA. EM david.fang@ars.usda.gov FU United States Department of Agriculture-Agricultural Research Service CRIS [6054-21000-017-00D]; Cotton Incorporated projects [10-747, 15-751] FX This research was funded by United States Department of Agriculture-Agricultural Research Service CRIS project 6054-21000-017-00D and Cotton Incorporated projects 10-747 and 15-751. NR 64 TC 1 Z9 1 U1 11 U2 11 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD NOV 9 PY 2016 VL 17 AR 903 DI 10.1186/s12864-016-3249-2 PG 17 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA EB5PH UT WOS:000387428000007 PM 27829353 ER PT J AU Liu, ZY Alseekh, S Brotman, Y Zheng, Y Fei, ZJ Tieman, DM Giovannoni, JJ Fernie, AR Klee, HJ AF Liu, Zhongyuan Alseekh, Saleh Brotman, Yariv Zheng, Yi Fei, Zhangjun Tieman, Denise M. Giovannoni, James J. Fernie, Alisdair R. Klee, Harry J. TI Identification of a Solanum pennellii Chromosome 4 Fruit Flavor and Nutritional Quality-Associated Metabolite QTL SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE flavor; fruit ripening; metabolome; transcriptome; IL4-4 ID TOMATO INTROGRESSION LINES; QUANTITATIVE TRAIT; MASS-SPECTROMETRY; TRANSCRIPTION FACTOR; EXPRESSION; DATABASE; GENES; YIELD; LOCI; METABOLOMICS AB A major resource for tomato quality improvement and gene discovery is the collection of introgression lines (ILs) of cultivated Solanum lycopersicurn that contain different, defined chromosomal segments derived from the wild tomato relative, S. pennellii, Among these lines, IL4-4, in which the bottom of S. Iycopersicum (cv. M82) chromosome 4 is replaced by the corresponding S. pennellii segment, is altered in many primary and secondary metabolites, including many related to fruit flavor and nutritional quality. Here, we provide a comprehensive profile of IL4-4 ripe fruit metabolites, the transcriptome and fine mapping of sub-ILs. Remarkably, out of 327 quantified metabolites, 185 were significantly changed in IL4-4 fruit, compared to the control. These altered metabolites include volatile organic compounds, primary and secondary metabolites. Partial least squares enhanced discriminant analysis of the metabolite levels among sub-ILs indicated that a genome region encompassing 20 putative open reading frames is responsible for most of the metabolic changes in IL4-4 fruit. This work provides comprehensive insights into IL4-4 fruit biochemistry, identifying a small region of the genome that has major effects on a large and diverse set of metabolites. C1 [Liu, Zhongyuan; Tieman, Denise M.; Klee, Harry J.] Univ Florida, Genet Inst, Dept Hort Sci, Gainesville, FL USA. [Alseekh, Saleh; Brotman, Yariv; Fernie, Alisdair R.] Max Planck Inst Mol Plant Physiol, Potsdam, Germany. [Brotman, Yariv] Ben Gurion Univ Negev, Dept Life Sci, Beer Sheva, Israel. [Zheng, Yi; Fei, Zhangjun; Giovannoni, James J.] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Zheng, Yi; Fei, Zhangjun; Giovannoni, James J.] Cornell Univ, Robert W Holley Ctr Agr & Hlth, Ithaca, NY USA. [Zheng, Yi; Fei, Zhangjun; Giovannoni, James J.] USDA ARS, Ithaca, NY 14853 USA. RP Klee, HJ (reprint author), Univ Florida, Genet Inst, Dept Hort Sci, Gainesville, FL USA. EM hjklee@ufl.edu RI Klee, Harry/A-7912-2008 NR 49 TC 0 Z9 0 U1 15 U2 15 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD NOV 9 PY 2016 VL 7 AR 1671 DI 10.3389/fpls.2016.01671 PG 15 WC Plant Sciences SC Plant Sciences GA EB3OZ UT WOS:000387276300001 PM 27881988 ER PT J AU Cantrell, CL Jones, AMP Ali, A AF Cantrell, Charles L. Jones, A. Maxwell P. Ali, Abbas TI Isolation and Identification of Mosquito (Aedes aegypti) Biting-Deterrent Compounds from the Native American Ethnobotanical Remedy Plant Hierochloe odorata (Sweetgrass) SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Hierochloe odorata; sweetgrass; ethnobotanical remedy; Aedes aegypti; repellent ID ESSENTIAL OIL; REPELLENT PLANTS; NATURAL-PRODUCTS; FATTY-ACIDS; COMPONENTS; LEAVES; L.; CONSTITUENTS; VOLATILES AB Hierochla odorata (L.) P. Beauv. (Poaceae), commonly known as sweetgrass, has documented use as an insect repellent by the Flatheads of Montana and Blackfoot of Alberta. Both the Flatheads of Montana and Blackfoot of Alberta would use braided plant material in a sachet in clothing or burn them from one end as incense, air/clothing freshener, and insect repellent. This study evaluated the insect-repellent properties of this plant using an in vitro mosquito Aedes aegypti feeding bioassay-directed approach to identify the compound(s) responsible for the observed activities. Evaluation of crude extracts produced from H. odorata revealed that the hydro distillate had the highest level of mosquito biting deterrence. Fractionation of this extract, followed by re-evaluation for mosquito biting deterrence, produced many active fractions, which were evaluated by spectroscopic techniques and determined to contain phytol, coumarin, and 2-methoxy-4-vinylphenol. Phytol and coumarin were both determined to be responsible for the Ae. aegypti biting deterrency. Scientific evidence reported here validates its traditional use as a biting-insect deterrent. C1 [Cantrell, Charles L.] Univ Mississippi, USDA ARS, Nat Prod Utilizat Res Unit, University, MS 38677 USA. [Jones, A. Maxwell P.] Univ Guelph, Gosling Res Inst Plant Preservat, Dept Plant Agr, Guelph, ON N1G 2W1, Canada. [Ali, Abbas] Univ Mississippi, Nat Ctr Nat Prod Res, University, MS 38677 USA. RP Cantrell, CL (reprint author), Univ Mississippi, USDA ARS, Nat Prod Utilizat Res Unit, University, MS 38677 USA. EM charles.cantrell@ars.usda.gov FU Deployed War-Fighter Protection (DWFP) research program grant - U.S. Department of Defense through the Armed Forces Pest Management Board; USDA-ARS Grant [56-6402-1-612] FX This study was supported in part by a Deployed War-Fighter Protection (DWFP) research program grant funded by the U.S. Department of Defense through the Armed Forces Pest Management Board and USDA-ARS Grant 56-6402-1-612. NR 46 TC 0 Z9 0 U1 10 U2 10 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD NOV 9 PY 2016 VL 64 IS 44 BP 8352 EP 8358 DI 10.1021/acs.jafc.6b01668 PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA EB8ES UT WOS:000387624900010 PM 27744691 ER PT J AU Smith, DJ Giddings, JM Herges, GR Ernst, W AF Smith, David J. Giddings, J. Michael Herges, Grant R. Ernst, William TI Distribution, Identification, and Quantification of Residues after Treatment of Ready-To-Eat Salami with (CI)-C-36-Labeled or Nonlabeled Chlorine Dioxide Gas SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE chlorine dioxide; chlorite; chlorate; perchlorate; residue; sanitation ID TOTAL RADIOACTIVE RESIDUES; LISTERIA-MONOCYTOGENES; PARENT CHLORATE; BEEF-CATTLE; SODIUM; INACTIVATION; TOMATOES; METABOLISM; CANTALOUPE; EFFICACY AB When ready-to-eat salami was treated in a closed system with (CI)-C-36-labeled ClO2 (5.5 mg/100 g of salami), essentially all radioactivity was deposited onto the salami. Administered (ClO2)-Cl-36 was converted to Cl-36-chloride ion (>97%), trace levels of chlorate (<2%), and detectable levels of chlorite. In residue studies conducted with nonlabeled ClO2, sodium perchlorate residues (LOQ, 4 ng/g) were not formed when reactions were protected from light. Sodium chlorate residues were present in control (39.2 +/- 4.8 ng/g) and chlorine dioxide treated (128 +/- 31.2 ng/g) salami. If sanitation occurred under conditions of illumination, detectable levels (3.7 +/- 1.5 ng/g) of perchlorate were formed along with greater quantities of sodium chlorate (183.6 +/- 75.4 ng/g). Collectively, these data suggest that ClO2 is chemically reduced by salami and that slow-release formulations might be appropriate for applications involving the sanitation of ready-to-eat meat products. C1 [Smith, David J.; Giddings, J. Michael; Herges, Grant R.] USDA ARS, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58102 USA. [Ernst, William] ICA Tri Nova Corp LLC, 24 Woodland Trail, Newnan, GA 30263 USA. RP Smith, DJ (reprint author), USDA ARS, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58102 USA. EM david.j.smith@ars.usda.gov NR 47 TC 0 Z9 0 U1 8 U2 8 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD NOV 9 PY 2016 VL 64 IS 44 BP 8454 EP 8462 DI 10.1021/acs.jafc.6b04011 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA EB8ES UT WOS:000387624900021 PM 27791367 ER PT J AU Zhang, ML Xiang, XG Xue, JJ Sanderson, SC Fritsch, PW AF Zhang, Ming-Li Xiang, Xiao-Guo Xue, Juan-Juan Sanderson, Stewart C. Fritsch, Peter W. TI Himalayan uplift shaped biomes in Miocene temperate Asia: evidence from leguminous Caragana SO SCIENTIFIC REPORTS LA English DT Article ID OLIGOCENE CLIMATE TRANSITION; TIBETAN PLATEAU; DIVERSIFICATION; ARIDIFICATION; EVOLUTION; RECORDS; CHINA; TIMES AB Caragana, with distinctive variation in leaf and rachis characters, exhibits three centers of geographic distribution, i.e., Central Asia, the Qinghai-Tibetan Plateau (QTP), and East Asia, corresponding to distinct biomes. Because Caragana species are often ecologically dominant components of the vegetation in these regions, it is regarded as a key taxon for the study of floristic evolution in the dry regions of temperate Asia. Based on an expanded data set of taxa and gene regions from those previously generated, we employed molecular clock and biogeographical analyses to infer the evolutionary history of Caragana and link it to floristic patterns, paleovegetation, and paleoclimate. Results indicate that Caragana is of arid origin from the Junggar steppe. Diversification of crown group Caragana, dated to the early Miocene ca. 18 Ma and onwards, can be linked to the Himalayan Motion stage of QTP uplift. Diversification of the major clades in the genus corresponding to taxonomic sections and morphological variation is inferred to have been driven by the uplift, as well as Asian interior aridification and East Asian monsoon formation, in the middle to late Miocene ca. 12 similar to 6 Ma. These findings demonstrate a synchronous evolution among floristics, vegetation and climate change in arid Central Asia, cold arid alpine QTP, and mesophytic East Asia. C1 [Zhang, Ming-Li; Xue, Juan-Juan] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, CAS Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Peoples R China. [Zhang, Ming-Li; Xiang, Xiao-Guo] Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China. [Sanderson, Stewart C.] US Forest Serv, Shrub Sci Lab, Intermt Res Stn, USDA, Provo, UT 84601 USA. [Fritsch, Peter W.] Bot Res Inst Texas, Ft Worth, TX 76107 USA. RP Zhang, ML (reprint author), Chinese Acad Sci, Xinjiang Inst Ecol & Geog, CAS Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Peoples R China.; Zhang, ML (reprint author), Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China. EM zhangml@ibcas.ac.cn FU National Basic Research Program of China [2014CB954201]; Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences FX This work was supported by the National Basic Research Program of China No. 2014CB954201 (M.-L.Z.), and Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences (M.-L.Z.). Two anonymous reviewers are deeply acknowledged for their helpful suggestions to the manuscript. NR 36 TC 0 Z9 0 U1 10 U2 10 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD NOV 9 PY 2016 VL 6 AR 36528 DI 10.1038/srep36528 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EB7ZC UT WOS:000387609300001 PM 27827446 ER PT J AU Yang, ZZ Zhang, YT Wafula, EK Honaas, LA Ralph, PE Jones, S Clarke, CR Liu, SM Su, C Zhang, HT Altman, NS Schuster, SC Timko, MP Yoder, JI Westwood, JH dePamphilis, CW AF Yang, Zhenzhen Zhang, Yeting Wafula, Eric K. Honaas, Loren A. Ralph, Paula E. Jones, Sam Clarke, Christopher R. Liu, Siming Su, Chun Zhang, Huiting Altman, Naomi S. Schuster, Stephan C. Timko, Michael P. Yoder, John I. Westwood, James H. dePamphilis, Claude W. TI Horizontal gene transfer is more frequent with increased heterotrophy and contributes to parasite adaptation SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE HGT; phylogenomics; validation pipeline; genomic transfer; parasitism ID MITOCHONDRIAL GENES; PHYLOGENETIC INFERENCE; OROBANCHE-AEGYPTIACA; ANGIOSPERM AMBORELLA; MAXIMUM-LIKELIHOOD; PLANT-MITOCHONDRIA; FLOWERING PLANTS; HOST; ARABIDOPSIS; EVOLUTION AB Horizontal gene transfer (HGT) is the transfer of genetic material across species boundaries and has been a driving force in prokaryotic evolution. HGT involving eukaryotes appears to bemuch less frequent, and the functional implications of HGT in eukaryotes are poorly understood. We test the hypothesis that parasitic plants, because of their intimate feeding contacts with host plant tissues, are especially prone to horizontal gene acquisition. We sought evidence of HGTs in transcriptomes of three parasitic members of Orobanchaceae, a plant family containing species spanning the full spectrum of parasitic capabilities, plus the free-living Lindenbergia. Following initial phylogenetic detection and an extensive validation procedure, 52 high-confidence horizontal transfer events were detected, often from lineages of known host plants and with an increasing number of HGT events in species with the greatest parasitic dependence. Analyses of intron sequences in putative donor and recipient lineages provide evidence for integration of genomic fragments far more often than retro-processed RNA sequences. Purifying selection predominates in functionally transferred sequences, with a small fraction of adaptively evolving sites. HGT-acquired genes are preferentially expressed in the haustorium-the organ of parasitic plants-and are strongly biased in predicted gene functions, suggesting that expression products of horizontally acquired genes are contributing to the unique adaptive feeding structure of parasitic plants. C1 [Yang, Zhenzhen; Honaas, Loren A.; Jones, Sam; Zhang, Huiting; dePamphilis, Claude W.] Penn State Univ, Intercoll Grad Program Plant Biol, Huck Inst Life Sci, University Pk, PA 16802 USA. [Yang, Zhenzhen; Zhang, Yeting; Wafula, Eric K.; Honaas, Loren A.; Ralph, Paula E.; Jones, Sam; Zhang, Huiting; dePamphilis, Claude W.] Penn State Univ, Dept Biol, University Pk, PA 16802 USA. [Yang, Zhenzhen; Zhang, Yeting; Wafula, Eric K.; Honaas, Loren A.; dePamphilis, Claude W.] Penn State Univ, Inst Mol Evolutionary Genet, Huck Inst Life Sci, University Pk, PA 16802 USA. [Zhang, Yeting; dePamphilis, Claude W.] Penn State Univ, Intercoll Grad Program Genet, Huck Inst Life Sci, University Pk, PA 16802 USA. [Clarke, Christopher R.; Westwood, James H.] Virginia Polytech Inst & State Univ, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. [Liu, Siming; Yoder, John I.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Su, Chun; Timko, Michael P.] Univ Virginia, Dept Biol, Charlottesville, VA 22904 USA. [Altman, Naomi S.] Penn State Univ, Dept Stat, University Pk, PA 16802 USA. [Altman, Naomi S.; Schuster, Stephan C.; dePamphilis, Claude W.] Penn State Univ, Huck Inst Life Sci, University Pk, PA 16802 USA. [Schuster, Stephan C.] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA. [Zhang, Yeting] Rutgers State Univ, Dept Genet, Piscataway, NJ 08854 USA. [Zhang, Yeting] Rutgers State Univ, Human Genet Inst New Jersey, Piscataway, NJ 08854 USA. [Honaas, Loren A.] Agr Res Serv, Tree Fruit Res Lab, USDA, Wenatchee, WA 98801 USA. RP dePamphilis, CW (reprint author), Penn State Univ, Intercoll Grad Program Plant Biol, Huck Inst Life Sci, University Pk, PA 16802 USA.; dePamphilis, CW (reprint author), Penn State Univ, Dept Biol, University Pk, PA 16802 USA.; dePamphilis, CW (reprint author), Penn State Univ, Inst Mol Evolutionary Genet, Huck Inst Life Sci, University Pk, PA 16802 USA.; dePamphilis, CW (reprint author), Penn State Univ, Intercoll Grad Program Genet, Huck Inst Life Sci, University Pk, PA 16802 USA.; dePamphilis, CW (reprint author), Penn State Univ, Huck Inst Life Sci, University Pk, PA 16802 USA. EM cwd3@psu.edu FU NSF Equipment Grant [MRI-1229046]; NSF Plant Genome Research Program [DBI-0701748, IOS-1238057]; Plant Biology graduate program; Genetics graduate program; Biology Department at Penn State University; National Institute of Food and Agriculture Project [131997]; NSF Grant [IOS-1213059] FX We thank Dr. Craig Praul and the Huck Genomics Core Facility for transcriptome sequencing and the gift of Striga genome sequences generated on a trial run of the Illumina HiSeq2500 sequencer that was purchased as NSF Equipment Grant MRI-1229046 (to C.W.d.); Tony Omeis for growing M. californica (with Grindelia host) in the Biology Greenhouse at Penn State University from material originally provided by Alison Colwell; K. Shirasu and S. Yoshida for access to the S. asiatica genome sequence and annotation; D. E. Soltis, M. K. Deyholos, M. W. Chase, and C. Wang for collecting nine of the 10 1KP samples used for HGT validation in this study; Ning Jiang (Michigan State University) for discussion of Pong-like TEs; and J. Naumann, J. Der, J. Palmer, M. Axtell, D. Cosgrove, S. Maximova, and M. Guiltinan for helpful suggestions. This research was supported by NSF Plant Genome Research Program Awards DBI-0701748 and IOS-1238057 (to J.H.W., C.W.d., M.P.T., and J.I.Y.), with additional support from the Plant Biology graduate program (Z.Y., L.A.H., S.J., and H.Z.) and from the Genetics graduate program as well as the Biology Department (Y.Z.) at Penn State University; National Institute of Food and Agriculture Project 131997 (to J.H.W.); and NSF Grant IOS-1213059 (to M.P.T.). NR 75 TC 0 Z9 0 U1 13 U2 13 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD NOV 8 PY 2016 VL 113 IS 45 BP E7010 EP E7019 DI 10.1073/pnas.1608765113 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EC4CL UT WOS:000388073300016 ER PT J AU Wetterhorn, KM Newmister, SA Caniza, RK Busman, M McCormick, SP Berthiller, F Adam, G Rayment, I AF Wetterhorn, Karl M. Newmister, Sean A. Caniza, Rachell K. Busman, Mark McCormick, Susan P. Berthiller, Franz Adam, Gerhard Rayment, Ivan TI Crystal Structure of Os79 (Os04g0206600) from Oryza sativa: A UDP-glucosyltransferase Involved in the Detoxification of Deoxynivalenol SO BIOCHEMISTRY LA English DT Article ID GLUCOSE ANTHOCYANIDIN 3-O-GLUCOSYLTRANSFERASE; FUSARIUM-GRAMINEARUM; MEDICAGO-TRUNCATULA; FUNCTIONAL-CHARACTERIZATION; TRICHOTHECENE BIOSYNTHESIS; MYCOTOXIN DEOXYNIVALENOL; HETEROLOGOUS EXPRESSION; CLITORIA-TERNATEA; GLYCOSYLTRANSFERASES; BARLEY AB Fusarium head blight is a plant disease with significant agricultural and health impact which affects cereal crops such as wheat, barley, and maize and is characterized by reduced grain yield and the accumulation of trichothecene mycotoxins such as deoxynivalenol (DON). Studies have identified trichothecene production as a virulence factor in Fusarium graminearum and have linked DON resistance to the ability to form DON-3-O-glucoside in wheat. Here, the structures of a deoxynivalenol:UDP-glucosyltransferase (Os79) from Oryza sativa are reported in complex with UDP in an open conformation, in complex with UDP in a closed conformation, and in complex with UDP-2-fluoro-2-deoxy-D-glucose and trichothecene at 1.8, 2.3, and 2.2 angstrom resolution, respectively. The active site of Os79 lies in a groove between the N-terminal acceptor and the C-terminal donor-binding domains. Structural alignments reveal that Os79 likely utilizes a catalytic mechanism similar to those of other plant UGTs, with His 27 activating the trichothecene 03 hydroxyl for nucleophilic attack at Cl' of the UDP-glucose donor. Kinetic analysis of mutant Os79 revealed that Thr 291 plays a critical role in catalysis as a catalytic acid or to position the UDP moiety during the nucleophilic attack. Steady-state kinetic analysis demonstrated that Os79 conjugates multiple trichothecene substrates such as DON, nivalenol, isotrichodermol, and HT-2 toxin, but not T-2 toxin. These data establish a foundation for understanding substrate specificity and activity in this enzyme and can be used to guide future efforts to increase DON resistance in cereal crops. C1 [Wetterhorn, Karl M.; Newmister, Sean A.; Caniza, Rachell K.; Rayment, Ivan] Univ Wisconsin, Dept Biochem, 433 Babcock Dr, Madison, WI 53706 USA. [Busman, Mark; McCormick, Susan P.] USDA ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Berthiller, Franz] Univ Nat Resources & Life Sci, Vienna BOKU, Dept Agrobiotechnol IFA Tulln, Christian Doppler Lab Mycotoxin Metab,Ctr Analyt, Konrad Lorenz Str 20, A-3430 Tulln, Austria. [Adam, Gerhard] Univ Nat Resources & Life Sci, Vienna BOKU, Dept Appl Genet & Cell Biol, Konrad Lorenz Str 24, A-3430 Tulln, Austria. [Newmister, Sean A.] Univ Michigan, Inst Life Sci, Ann Arbor, MI 48109 USA. RP Rayment, I (reprint author), Univ Wisconsin, Dept Biochem, 433 Babcock Dr, Madison, WI 53706 USA. EM ivan_rayment@biochem.wisc.edu FU National Institute of Food and Agriculture, U.S. Department of Agriculture Hatch Project [1006542]; U.S. Department of Agriculture [59-0206-1-117]; Vienna Science and Technology Fund [LS12-021]; Austrian Science Fund FWF (SFB Fusarium); U.S. Department of Energy, Office of Energy Research [W-31-109-ENG-38] FX This work was supported in part by the National Institute of Food and Agriculture, U.S. Department of Agriculture Hatch Project 1006542, and the U.S. Department of Agriculture, under Agreement 59-0206-1-117. This is a cooperative project with the U.S. Wheat & Barley Scab Initiative (I.R.). The project was supported by the Vienna Science and Technology Fund (LS12-021) and the Austrian Science Fund FWF (SFB Fusarium). Use of the Structural Biology ID19 and BM19 beamlines (Advanced Photon Source, Argonne National Laboratory) was supported by the U.S. Department of Energy, Office of Energy Research, under Contract W-31-109-ENG-38. NR 55 TC 0 Z9 0 U1 9 U2 9 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD NOV 8 PY 2016 VL 55 IS 44 BP 6175 EP 6186 DI 10.1021/acs.biochem.6b00709 PG 12 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EB6TQ UT WOS:000387518900009 PM 27715009 ER PT J AU Frazier, TP Palmer, NA Xie, FL Tobias, CM Donze-Reiner, TJ Bombarely, A Childs, KL Shu, SQ Jenkins, JW Schmutz, J Zhang, BH Sarath, G Zhao, BY AF Frazier, Taylor P. Palmer, Nathan A. Xie, Fuliang Tobias, Christian M. Donze-Reiner, Teresa J. Bombarely, Aureliano Childs, Kevin L. Shu, Shengqiang Jenkins, Jerry W. Schmutz, Jeremy Zhang, Baohong Sarath, Gautam Zhao, Bingyu TI Identification, characterization, and gene expression analysis of nucleotide binding site (NB)-type resistance gene homologues in switchgrass SO BMC GENOMICS LA English DT Article DE Biofuel; Disease resistance; Gene expression; NB-LRR; Panicum virgatum (switchgrass); RNA-seq; SNP ID NBS-LRR GENES; GENOME-WIDE ANALYSIS; RICH REPEAT GENES; DISEASE-RESISTANCE; PUCCINIA-EMACULATA; SEQUENCE ALIGNMENTS; DEFENSE RESPONSES; NETWORK ANALYSIS; PROTEIN DOMAIN; HOST-DEFENSE AB Background: Switchgrass (Panicum virgatum L.) is a warm-season perennial grass that can be used as a second generation bioenergy crop. However, foliar fungal pathogens, like switchgrass rust, have the potential to significantly reduce switchgrass biomass yield. Despite its importance as a prominent bioenergy crop, a genome-wide comprehensive analysis of NB-LRR disease resistance genes has yet to be performed in switchgrass. Results: In this study, we used a homology-based computational approach to identify 1011 potential NB-LRR resistance gene homologs (RGHs) in the switchgrass genome (v 1.1). In addition, we identified 40 RGHs that potentially contain unique domains including major sperm protein domain, jacalin-like binding domain, calmodulin-like binding, and thioredoxin. RNA-sequencing analysis of leaf tissue from 'Alamo', a rust-resistant switchgrass cultivar, and 'Dacotah', a rust-susceptible switchgrass cultivar, identified 2634 high quality variants in the RGHs between the two cultivars. RNA-sequencing data from field-grown cultivar 'Summer' plants indicated that the expression of some of these RGHs was developmentally regulated. Conclusions: Our results provide useful insight into the molecular structure, distribution, and expression patterns of members of the NB-LRR gene family in switchgrass. These results also provide a foundation for future work aimed at elucidating the molecular mechanisms underlying disease resistance in this important bioenergy crop. C1 [Frazier, Taylor P.; Bombarely, Aureliano; Zhao, Bingyu] Virginia Tech, Dept Hort, Blacksburg, VA 24061 USA. [Palmer, Nathan A.; Sarath, Gautam] USDA ARS, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. [Xie, Fuliang; Zhang, Baohong] East Carolina Univ, Dept Biol, Greenville, NC 27858 USA. [Tobias, Christian M.] USDA ARS, Crop Improvement & Genet Res, Albany, CA 94710 USA. [Donze-Reiner, Teresa J.] West Chester Univ Penn, Dept Biol, Wester Chester, PA 19382 USA. [Childs, Kevin L.] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. [Shu, Shengqiang] Joint Genome Inst, Dept Energy, Walnut Creek, CA 94595 USA. [Jenkins, Jerry W.; Schmutz, Jeremy] HudsonAlpha Inst Biotechnol, Huntsville, AL 35806 USA. [Zhao, Bingyu] 407 Latham Hall,220 Ag Quad Lane, Blacksburg, VA 24061 USA. RP Zhao, BY (reprint author), Virginia Tech, Dept Hort, Blacksburg, VA 24061 USA.; Zhao, BY (reprint author), 407 Latham Hall,220 Ag Quad Lane, Blacksburg, VA 24061 USA. EM bzhao07@vt.edu OI Bombarely, Aureliano/0000-0001-6257-8914 FU USDA-NIFA [2011-67009-30133, 2011-67009-30096]; Virginia Tech CALS integrative grant; Virginia Agricultural Experiment Station [VA135872]; Office of Science (BER), U. S. Department of Energy [DE-AI02-09ER64829]; USDA-ARS CRIS project [3042-21000-030-00D]; Office of Science of the US Department of Energy [DE-AC02-05CH11231] FX The project was supported by USDA-NIFA Grant Number 2011-67009-30133. The project was also partially supported by a Virginia Tech CALS integrative grant and the Virginia Agricultural Experiment Station (VA135872). Work performed by the ARS was supported in part by the Office of Science (BER), U. S. Department of Energy Grant Number DE-AI02-09ER64829, USDA-NIFA Grant Number 2011-67009-30096, and by the USDA-ARS CRIS project 3042-21000-030-00D. The U. S. Department of Agriculture, Agricultural Research Service, is an equal opportunity/affirmative action employer and all agency services are available without discrimination. Mention of commercial products and organizations in this manuscript is solely to provide specific information. It does not constitute endorsement by USDA-ARS over other products and organizations not mentioned. The work conducted by the US Department of Energy Joint Genome Institute is supported by the Office of Science of the US Department of Energy under contract number DE-AC02-05CH11231. NR 75 TC 0 Z9 0 U1 11 U2 11 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD NOV 8 PY 2016 VL 17 AR 892 DI 10.1186/s12864-016-3201-5 PG 17 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA EB2IQ UT WOS:000387183300002 PM 27821048 ER PT J AU Juneja, VK Valenzuela-Melendres, M Heperkan, D Bautista, D Anderson, D Hwang, CA Pena-Ramos, A Camou, JP Torrentera-Olivera, N AF Juneja, Vijay K. Valenzuela-Melendres, Martin Heperkan, Dilek Bautista, Derrick Anderson, David Hwang, Cheng-An Pena-Ramos, Aida Pedro Camou, Juan Torrentera-Olivera, Noemi TI Development of a predictive model for Salmonella spp. reduction in meat jerky product with temperature, potassium sorbate, pH, and water activity as controlling factors SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Salmonella; Quantitative microbiology; Beef jerky; Temperature ID RESPONSE-SURFACE METHODOLOGY; ESCHERICHIA-COLI O157/H7; LISTERIA-MONOCYTOGENES; STAPHYLOCOCCUS-AUREUS; PACKAGED BEEF; INACTIVATION; GROWTH; FRESH; OPTIMIZATION; INHIBITION AB The objective of this study was to develop a predictive model for the inactivation of Salmonella spp. in ground beef jerky as a function of temperature (T), pH, potassium sorbate (PS), and final water activity (a(w)). Following a central composite design, ground beef was combined with PS (0 to 03%, w/w), pH adjusted from 5 to 7, inoculated with a cocktail of 6 serotypes of Salmonella spp. and heat processed at temperatures between 65 and 85 degrees C until the final a(w) ranging from 0.65 to 0.85 was achieved. Surviving Salmonella cells were enumerated on tryptic soy agar overlaid with xylose lysine deoxycholate agar (pre-tempered to 47 degrees C) after incubation for 48 h at 30 degrees C. Bacterial inactivation was quantified in terms of logarithmic reductions of Salmonella counts (log(10) CFU/g) and inactivation rate (log(10) (CFU/g)/h). The results indicated that pH, PS and T significantly (p < 0.05) interacted to inactivate Salmonella in beef jerky. Decreasing meat pH significantly (p < 0.05) increased the efficacy of PS and T to reduce the levels of Salmonella spp. Beef jerky processed at 82 degrees C, pH 5.5, with 0.25% PS to a final aw of 0.7 resulted in a maximum Salmonella logarithmic reduction of 5.0 log(10) CFU/g and an inactivation rate of 13 log(10) (CFU/g)/h. The predictive model developed can be used to effectively design drying processes for beef jerky under low humidity conditions and thereby, ensuring an adequate degree of protection against risks associated with Salmonella spp. (C) 2016 Published by Elsevier B.V. C1 [Juneja, Vijay K.; Hwang, Cheng-An] USDA ARS, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19308 USA. [Valenzuela-Melendres, Martin; Pena-Ramos, Aida; Pedro Camou, Juan] Ctr Invest Alimentac & Desarrollo AC, Lab Ciencia & Tecnol Carne, Carretera Victoria Km 0-6, Hermosillo 83304, Sonora, Mexico. [Heperkan, Dilek] Istanbul Tech Univ, Dept Food Engn, TR-34469 Istanbul, Turkey. [Bautista, Derrick] Del Monte Foods Inc, 205 N Wiget Ln, Walnut Creek, CA USA. [Anderson, David] Big Heart Pet Brands, 375 North Shore Dr, Pittsburgh, PA 15212 USA. [Torrentera-Olivera, Noemi] Univ Autonoma Baja California, Inst Ciencias Agr, Carretera Delta Oaxaca S-N, Valle De Mexicali 21705, Baja California, Mexico. RP Juneja, VK (reprint author), USDA ARS, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19308 USA. EM vijay.juneja@ars.usda.gov NR 23 TC 0 Z9 0 U1 22 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD NOV 7 PY 2016 VL 236 BP 1 EP 8 DI 10.1016/j.ijfoodmicro.2016.06.028 PG 8 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA DY1KV UT WOS:000384854800001 PM 27427870 ER PT J AU McPherson, MC Cheng, HH Delany, ME AF McPherson, Marla C. Cheng, Hans H. Delany, Mary E. TI Marek's disease herpesvirus vaccines integrate into chicken host chromosomes yet lack a virus-host phenotype associated with oncogenic transformation SO VACCINE LA English DT Article DE Marek's disease virus; Alphaherpesvirus; Chicken; Vaccine; Viral integration; Cytogenetics ID BACTERIAL ARTIFICIAL CHROMOSOMES; ATTENUATED STRAINS; CELL-CULTURE; TURKEYS HVT; MEQ GENE; REPLICATION; GENOME; VACCINATION; PROTECTION; TELOMERASE AB Marek's disease (MD) is a lymphotropic and oncogenic disease of chickens that can lead to death in susceptible and unvaccinated host birds. The causative pathogen, MD virus (MDV), a highly oncogenic alpha-herpesvirus, integrates into host genome near the telomeres. MD occurrence is controlled across the globe by bipsecurity, selective breeding for enhanced MD genetic resistance, and widespread vaccination of flocks using attenuated serotype 1 MDV or other serotypes. Despite over 40 years of usage, the specific mechanism(s) of MD vaccine-related immunity and anti-tumor effects are not known. Here we investigated the cytogenetic interactions of commonly used MD vaccine strains of all three serotypes (HVT, SB-1, and Rispens) with the host to determine if all were equally capable of host genome integration. We also studied the dynamic profiles of chromosomal association and integration of the three vaccine strains, a first for MD vaccine research. Our cytogenetic data provide evidence that all three MD vaccine strains tested integrate in the chicken host genome as early as 1 day after vaccination similar to oncogenic strains. However, a specific, transformation-associated virus-host phenotype observed for oncogenic viruses is not established. Our results collectively provide an updated model of MD vaccine-host genome interaction and an improved understanding of the possible mechanisms of vaccinal immunity. Physical integration of the oncogenic MDV genome into host chromosomes along with cessation of viral replication appears to have joint signification in MDV's ability to induce oncogenic transformation. Whereas for MD vaccine serotypes, a sustained viral replication stage and lack of the chromosome integrated only stage were shared traits during early infection. (C) 2016 The Author(s). Published by Elsevier Ltd. C1 [McPherson, Marla C.; Delany, Mary E.] Univ Calif Davis, Dept Anim Sci, One Shields Ave, Davis, CA 95616 USA. [Cheng, Hans H.] ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. RP Delany, ME (reprint author), Univ Calif Davis, Dept Anim Sci, One Shields Ave, Davis, CA 95616 USA. EM medelany@ucdavis.edu FU USDA National Institute of Food and Agriculture (NIFA) [2013-67015-21330, 2005-35205-16679]; National Research Support Program-8 [CA-D*-ASC-7233-RR]; Multi-State Research Projects [NC-1170 (CA-D*-ASC-6414-RR), NE-1334 (CA-D*-ASC-7281-RR)] FX This project was supported by the USDA National Institute of Food and Agriculture (NIFA) Grant nos. 2013-67015-21330 and 2005-35205-16679, National Research Support Program-8 (CA-D*-ASC-7233-RR), and Multi-State Research Projects NC-1170 (CA-D*-ASC-6414-RR) and NE-1334 (CA-D*-ASC-7281-RR). The authors acknowledge the infrastructure support of the Department of Animal Science and the College of Agricultural and Environmental Sciences and the California Agricultural Experiment Station at the University of California, Davis. We also greatly appreciate the research contributions of Dr. Charmaine Robinson and Ms. Laurie Molitor. NR 57 TC 0 Z9 0 U1 5 U2 5 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X EI 1873-2518 J9 VACCINE JI Vaccine PD NOV 4 PY 2016 VL 34 IS 46 BP 5554 EP 5561 DI 10.1016/j.vaccine.2016.09.051 PG 8 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA EA9TT UT WOS:000386988300015 PM 27720297 ER PT J AU Lu, MM Krutovsky, KV Nelson, CD Koralewski, TE Byram, TD Loopstra, CA AF Lu, Mengmeng Krutovsky, Konstantin V. Nelson, C. Dana Koralewski, Tomasz E. Byram, Thomas D. Loopstra, Carol A. TI Exome genotyping, linkage disequilibrium and population structure in loblolly pine (Pinus taeda L.) (vol 17, 730, 2016) SO BMC GENOMICS LA English DT Correction C1 [Lu, Mengmeng; Krutovsky, Konstantin V.; Koralewski, Tomasz E.; Byram, Thomas D.; Loopstra, Carol A.] Texas A&M Univ, Dept Ecosyst Sci & Management, 2138 TAMU, College Stn, TX 77843 USA. [Lu, Mengmeng; Krutovsky, Konstantin V.; Loopstra, Carol A.] Texas A&M Univ, Mol & Environm Plant Sci Program, 2138 TAMU, College Stn, TX 77843 USA. [Krutovsky, Konstantin V.] Georg August Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, D-37077 Gottingen, Germany. [Krutovsky, Konstantin V.] Russian Acad Sci, NI Vavilov Inst Gen Genet, Gubkina Str, Moscow 119333, Russia. [Krutovsky, Konstantin V.] Siberian Fed Univ, Genome Res & Educ Ctr, 50a-2 Akademgorodok, Krasnoyarsk 660036, Russia. [Nelson, C. Dana] Forest Serv, USDA, Southern Res Stn, Southern Inst Forest Genet, 23332 Success Rd, Saucier, MS 39574 USA. [Nelson, C. Dana] Univ Kentucky, Forest Hlth Res & Educ Ctr, 730 Rose St, Lexington, KY 40546 USA. [Byram, Thomas D.] Texas A&M Forest Serv, 2585 TAMU, College Stn, TX 77843 USA. RP Krutovsky, KV (reprint author), Texas A&M Univ, Dept Ecosyst Sci & Management, 2138 TAMU, College Stn, TX 77843 USA.; Krutovsky, KV (reprint author), Texas A&M Univ, Mol & Environm Plant Sci Program, 2138 TAMU, College Stn, TX 77843 USA.; Krutovsky, KV (reprint author), Georg August Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, D-37077 Gottingen, Germany.; Krutovsky, KV (reprint author), Russian Acad Sci, NI Vavilov Inst Gen Genet, Gubkina Str, Moscow 119333, Russia.; Krutovsky, KV (reprint author), Siberian Fed Univ, Genome Res & Educ Ctr, 50a-2 Akademgorodok, Krasnoyarsk 660036, Russia. EM konstantin.krutovsky@forst.uni-goettingen.de NR 1 TC 0 Z9 0 U1 3 U2 3 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD NOV 4 PY 2016 VL 17 AR 869 DI 10.1186/s12864-016-3220-2 PG 1 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA EA9NL UT WOS:000386970800001 PM 27814686 ER PT J AU Filgueiras, CC Willett, DS Pereira, RV Moino, A Pareja, M Duncan, LW AF Filgueiras, Camila Cramer Willett, Denis S. Pereira, Ramom Vasconcelos Moino Junior, Alcides Pareja, Martin Duncan, Larry W. TI Eliciting maize defense pathways aboveground attracts belowground biocontrol agents SO SCIENTIFIC REPORTS LA English DT Article ID DIABROTICA-SPECIOSA GERMAR; ENTOMOPATHOGENIC NEMATODES; HETERORHABDITIS-AMAZONENSIS; PLANT; JASMONATE; RHABDITIDA; SALICYLATE; HERBIVORES; CHRYSOMELIDAE; COLEOPTERA AB Plant defense pathways mediate multitrophic interactions above and belowground. Understanding the effects of these pathways on pests and natural enemies above and belowground holds great potential for designing effective control strategies. Here we investigate the effects of aboveground stimulation of plant defense pathways on the interactions between corn, the aboveground herbivore adult Diabrotica speciosa, the belowground herbivore larval D. speciosa, and the subterranean entomopathogenic nematode natural enemy Heterorhabditis amazonensis. We show that adult D. speciosa recruit to aboveground herbivory and methyl salicylate treatment, that larval D. speciosa are relatively indiscriminate, and that H. amazonensis en-tomopathogenic nematodes recruit to corn fed upon by adult D. speciosa. These results suggest that entomopathogenicnematodes belowground can be highly attuned to changes in the aboveground parts of plants and that biological control can be enhanced with induced plant defense in this and similar systems. C1 [Filgueiras, Camila Cramer; Pereira, Ramom Vasconcelos; Moino Junior, Alcides] Univ Fed Lavras, Dept Entomol, BR-37200000 Lavras, Brazil. [Willett, Denis S.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Pareja, Martin] Univ Estadual Campinas UNICAMP, Inst Biol, Dept Biol Anim, BR-13083970 Campinas, SP, Brazil. [Duncan, Larry W.] Univ Florida, Citrus Res & Educ Ctr, Dept Entomol & Nematol, Lake Alfred, FL 33850 USA. RP Filgueiras, CC (reprint author), Univ Fed Lavras, Dept Entomol, BR-37200000 Lavras, Brazil. EM camilacramer@gmail.com RI Pareja, Martin/A-9982-2012 FU Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) program; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq); FAEPEX-PAPDIC grant from UNICAMP FX We thank Maria Eduarda C.F.R. Silva who assisted with and provided invaluable support for all aspects of the project. The Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) program and the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) provided financial support for the project through studentships and grants to CCF. MP was supported by a FAEPEX-PAPDIC grant from UNICAMP. NR 46 TC 0 Z9 0 U1 10 U2 10 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD NOV 4 PY 2016 VL 6 AR 36484 DI 10.1038/srep36484 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EB0GV UT WOS:000387022900001 PM 27811992 ER PT J AU Shriner, SA Root, JJ Lutman, MW Kloft, JM VanDalen, KK Sullivan, HJ White, TS Milleson, MP Hairston, JL Chandler, SC Wolf, PC Turnage, CT McCluskey, BJ Vincent, AL Torchetti, MK Gidlewski, T DeLiberto, TJ AF Shriner, Susan A. Root, J. Jeffrey Lutman, Mark W. Kloft, Jason M. VanDalen, Kaci K. Sullivan, Heather J. White, Timothy S. Milleson, Michael P. Hairston, Jerry L. Chandler, Shannon C. Wolf, Paul C. Turnage, Clinton T. McCluskey, Brian J. Vincent, Amy L. Torchetti, Mia K. Gidlewski, Thomas DeLiberto, Thomas J. TI Surveillance for highly pathogenic H5 avian influenza virus in synanthropic wildlife associated with poultry farms during an acute outbreak SO SCIENTIFIC REPORTS LA English DT Article ID SPARROWS PASSER-DOMESTICUS; STARLINGS STURNUS-VULGARIS; EUROPEAN STARLINGS; A VIRUSES; EXPERIMENTAL-INFECTION; A(H7N9) VIRUS; TREE SPARROW; BIRDS; TRANSMISSION; ANTIBODIES AB In November 2014, a Eurasian strain H5N8 highly pathogenic avian influenza virus was detected in poultry in Canada. Introduced viruses were soon detected in the United States and within six months had spread to 21 states with more than 48 million poultry affected. In an effort to study potential mechanisms of spread of the Eurasian H5 virus, the United States Department of Agriculture coordinated several epidemiologic investigations at poultry farms. As part of those efforts, we sampled synanthropic birds and mammals at five infected and five uninfected poultry farms in northwest Iowa for exposure to avian influenza viruses. Across all farms, we collected 2,627 samples from 648 individual birds and mammals. House mice were the most common mammal species captured while house sparrows, European starlings, rock pigeons, swallows, and American robins were the most commonly captured birds. A single European starling was positive for Eurasian H5 viral RNA and seropositive for antibodies reactive to the Eurasian H5 virus. Two American robins were also seropositive. No mammal species showed evidence of infection. These results indicate synanthropic species merit further scrutiny to better understand potential biosecurity risks. We propose a set of management practices aimed at reducing wildlife incursions. C1 [Shriner, Susan A.; Root, J. Jeffrey; Lutman, Mark W.; VanDalen, Kaci K.; Sullivan, Heather J.; Gidlewski, Thomas; DeLiberto, Thomas J.] USDA APHIS, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Kloft, Jason M.] USDA APHIS, Wildlife Serv, Manhattan, KS USA. [White, Timothy S.] USDA APHIS, Wildlife Serv, Springfield, IL USA. [Milleson, Michael P.] USDA APHIS, Wildlife Serv, Gainesville, FL USA. [Hairston, Jerry L.] USDA APHIS, Wildlife Serv, Raleigh, NC USA. [Chandler, Shannon C.] USDA APHIS, Wildlife Serv, Sacramento, CA USA. [Wolf, Paul C.] USDA APHIS, Wildlife Serv, Minneapolis, MN USA. [Turnage, Clinton T.] USDA APHIS, Wildlife Serv, Sherwood, AR USA. [McCluskey, Brian J.] USDA APHIS, Sci Technol & Anal Serv, Vet Serv, Ft Collins, CO USA. [Vincent, Amy L.] USDA ARS, Virus & Prion Dis Livestock Res Unit, Natl Anim Dis Ctr, Ames, IA USA. [Torchetti, Mia K.] USDA APHIS, Natl Vet Serv Labs, Technol & Anal Serv, Vet Serv, Ames, IA USA. RP Shriner, SA (reprint author), USDA APHIS, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. EM Susan.A.Shriner@aphis.usda.gov FU poultry industry FX We are very appreciative of the cooperation and support of the poultry industry for allowing us access to their properties and for on-site support. We thank Jeremy Ellis (NWRC) for producing the maps in Figures 1 and 2, Jan Pedersen (NVSL) and Julie Reinsch (NVSL) for work on the HI/NI testing, and Hailey McLean (NWRC), Katie Dirsmith (NWRC), and Tyler Cochran (NWRC) for supply and laboratory support. NR 64 TC 0 Z9 0 U1 10 U2 10 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD NOV 4 PY 2016 VL 6 AR 36237 DI 10.1038/srep36237 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EB0DM UT WOS:000387013600001 PM 27812044 ER PT J AU Sanad, MNME Campbell, KG Gill, KS AF Sanad, Marwa N. M. E. Campbell, Kimberley Garland Gill, Kulvinder S. TI Developmental program impacts phenological plasticity of spring wheat under drought SO BOTANICAL STUDIES LA English DT Article DE Phenological plasticity; Developmental plasticity; Tillering stage; Drought; Spring wheat ID CHLOROPHYLL FLUORESCENCE; PHENOTYPIC PLASTICITY; HIGH-TEMPERATURE; PLANT-GROWTH; WATER-STRESS; GRAIN YIELD; RHT ALLELES; MECHANISMS; TOLERANCE; GENES AB Background: Developing drought-tolerant crops critically depends on the efficient response of a genotype to the limited water availability, a trait known as phenological plasticity. Our understanding of the phenological plasticity remains limited, in particular, about its relationships with plant developmental program. Here, we examined the plastic response of spring wheat at tillering, booting, heading, and anthesis stages to constant or periodic drought stress. The response was assessed by morphological and physiological parameters including symptoms. Results: The dynamics of morphological symptoms were indicators of the plasticity identification of drought. We found that spring wheat exhibits higher phenological plasticity during tillering stage followed by the heading stage, while booting and anthesis stages are the most sensitive. Also, the adaptive response is thought to be influenced with the plant height genes. Furthermore, periodic stress caused more pronounced inhibition of yield than the constant stress, with limited resistance resolution under long period. Conclusions: Our study shows the importance of considering the phenological plasticity in designing screens for drought tolerance in spring wheat and proposes tillering as the most informative stage for capturing genotypes with tolerance to limit water availability. C1 [Sanad, Marwa N. M. E.; Campbell, Kimberley Garland; Gill, Kulvinder S.] Washington State Univ, Dept Soil & Crop Sci, Pullman, WA 99164 USA. [Sanad, Marwa N. M. E.] Natl Res Ctr, Genet & Cytol Dept, Giza, Egypt. [Campbell, Kimberley Garland] USDA ARS, Wheat Genet Qual Physiol Dis Res, Pullman, WA USA. RP Sanad, MNME (reprint author), Washington State Univ, Dept Soil & Crop Sci, Pullman, WA 99164 USA.; Sanad, MNME (reprint author), Natl Res Ctr, Genet & Cytol Dept, Giza, Egypt. EM marwa.sanad@wsu.edu FU Egyptian government in Dr. Kulvinder Gill's lab at Washington State University, Pullman, WA, USA FX Majority of this work was done through a postdoctoral fellowship for Dr. Marwa Sanad from the Egyptian government in Dr. Kulvinder Gill's lab at Washington State University, Pullman, WA, USA. NR 43 TC 0 Z9 0 U1 5 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1999-3110 J9 BOT STUD JI Bot. Stud. PD NOV 3 PY 2016 VL 57 AR 35 DI 10.1186/s40529-016-0149-3 PG 12 WC Plant Sciences SC Plant Sciences GA ED1DI UT WOS:000388583800001 ER PT J AU Paris, TM Allan, SA Hall, DG Hentz, MG Hetesy, G Stansly, PA AF Paris, Thomson M. Allan, Sandra A. Hall, David G. Hentz, Matthew G. Hetesy, Gabriella Stansly, Philip A. TI Host plant affects morphometric variation of Diaphorina citri (Hemiptera: Liviidae) SO PEERJ LA English DT Article DE ACP; Asian citrus psyllid; Morphometrics; Host plant; Huanglongbing ID GEOMETRIC MORPHOMETRICS; PHENOTYPIC VARIATION; ABDOMINAL COLOR; MUSCA-DOMESTICA; BODY-SIZE; PSYLLIDAE; POPULATIONS; BIOLOGY; FLIGHT; VECTOR AB The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, is one of the most serious citrus pests worldwide due to its role as vector of huanglongbing or citrus greening disease. While some optimal plant species for ACP oviposition and development have been identified, little is known of the influence of host plants on ACP size and shape. Our goal was to determine how size and shape of ACP wing and body size varies when development occurs on different host plants in a controlled rearing environment. ACP were reared on six different rutaceous species; Bergera koenigii, Citrus aurantifolia, Citrus macrophylla, Citrus maxima, Citrus taiwanica and Murraya paniculata. Adults were examined for morphometric variation using traditional and geometric analysis based on 12 traits or landmarks. ACP reared on C. taiwanica were consistently smaller than those reared on the other plant species. Wing aspect ratio also differed between C. maxima and C. taiwanica. Significant differences in shape were detected with those reared on M. paniculata having narrower wings than those reared on C. macrophylla. This study provides evidence of wing size and shape differences of ACP based on host plant species which potentially may impact dispersal. Further study is needed to determine if behavioral and physiological differences are associated with the observed phenotypic differences. C1 [Paris, Thomson M.; Allan, Sandra A.; Hetesy, Gabriella] ARS, USDA, Gainesville, FL 32667 USA. [Paris, Thomson M.; Stansly, Philip A.] Univ Florida, Southwest Florida Res & Educ Ctr, Immokalee, FL USA. [Hall, David G.; Hentz, Matthew G.] ARS, USDA, Ft Pierce, FL USA. RP Allan, SA (reprint author), ARS, USDA, Gainesville, FL 32667 USA. EM sandy.allan@ars.usda.gov FU Citrus Research and Development Foundation [701] FX Funding was provided by the Citrus Research and Development Foundation (701). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 81 TC 1 Z9 1 U1 9 U2 9 PU PEERJ INC PI LONDON PA 341-345 OLD ST, THIRD FLR, LONDON, EC1V 9LL, ENGLAND SN 2167-8359 J9 PEERJ JI PeerJ PD NOV 3 PY 2016 VL 4 AR e2663 DI 10.7717/peerj.2663 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EB2EP UT WOS:000387170800011 PM 27833820 ER PT J AU Nimmakayala, P Abburi, VL Saminathan, T Almeida, A Davenport, B Davidson, J Reddy, CVCM Hankins, G Ebert, A Choi, D Stommel, J Reddy, UK AF Nimmakayala, Padma Abburi, Venkata L. Saminathan, Thangasamy Almeida, Aldo Davenport, Brittany Davidson, Joshua Reddy, C. V. Chandra Mohan Hankins, Gerald Ebert, Andreas Choi, Doil Stommel, John Reddy, Umesh K. TI Genome-Wide Divergence and Linkage Disequilibrium Analyses for Capsicum baccatum Revealed by Genome-Anchored Single Nucleotide Polymorphisms SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE population structure; linkage disequilibrium; haplotyping; genotyping by sequencing; genome-wide association mapping; peduncle length ID POPULATION-STRUCTURE; GENETIC DIVERSITY; PEPPER CULTIVARS; CELL ELONGATION; GENUS CAPSICUM; CHILI-PEPPER; ANNUUM L.; ASSOCIATION; SOLANACEAE; EVOLUTION AB Principal component analysis (PCA) with 36,621 polymorphic genome-anchored single nucleotide polymorphisms (SNPs) identified collectively for Capsicum annuum and Capsicum baccaturn was used to characterize population structure and species domestication of these two important incompatible cultivated pepper species. Estimated mean nucleotide diversity (pi) and Tajima's D across various chromosomes revealed biased distribution toward negative values on all chromosomes (except for chromosome 4) in cultivated C. baccatum, indicating a population bottleneck during domestication of C. baccatum. In contrast, C. annuurn chromosomes showed positive 7 and Tajima's D on all chromosomes except chromosome 8, which may be because of domestication at multiple sites contributing to wider genetic diversity. For C. baccatum, 13,129 SNPs were available, with minor allele frequency (MAF) >= 0.05; PCA of the SNPs revealed 283 C. baccatum accessions grouped into 3 distinct clusters, for strong population structure. The fixation index (F-ST) between domesticated C. annuum and C. baccatum was 0.78, which indicates genome-wide divergence. We conducted extensive linkage disequilibrium (LD) analysis of C. baccaturn var. pendulum cultivars on all adjacent SNP pairs within a chromosome to identify regions of high and low LD interspersed with a genome-wide average LD block size of 99.1 kb. We characterized 1742 haplotypes containing 4420 SNPs (range 9-2 SNPs per haplotype). Genome-wide association study (GWAS) of peduncle length, a trait that differentiates wild and domesticated C. baccatum types, revealed 36 significantly associated genome-wide SNPs. Population structure, identity by state (IBS) and LD patterns across the genome will be of potential use for future GWAS of economically important traits in C. baccatum peppers. C1 [Nimmakayala, Padma; Abburi, Venkata L.; Saminathan, Thangasamy; Almeida, Aldo; Davenport, Brittany; Davidson, Joshua; Reddy, C. V. Chandra Mohan; Hankins, Gerald; Reddy, Umesh K.] West Virginia State Univ, Gus R Douglass Inst, Dept Biol, Institute, WV 25112 USA. [Ebert, Andreas] Asian Vegetable Res & Dev Ctr, Genet Resources & Seed Unit, World Vegetable Ctr, Tainan, Taiwan. [Choi, Doil] Seoul Natl Univ, Plant Genom & Breeding Inst, Dept Plant Sci, Coll Agr & Life Sci, Seoul, South Korea. [Stommel, John] ARS, Genet Improvement Fruits & Vegetables Lab, USDA, Beltsville, MD 1 USA. RP Reddy, UK (reprint author), West Virginia State Univ, Gus R Douglass Inst, Dept Biol, Institute, WV 25112 USA. EM ureddy@wvstateu.edu RI Choi, Doil/F-5002-2011 FU USDA-NIFA [2010-02419, 2012-02617]; NIH [P20RR016477]; University Grants Commission, Government of India; Gus R. Douglass Institute; Agricultural Genome Center of Next-Generation Biogreen 21 Program [PJ011275] FX The study received funding from USDA-NIFA (2010-02419 and 2012-02617), NIH Grant P20RR016477 to the West Virginia IDeA Network for Biomedical Research Funding, Raman postdoctoral fellowship to CR by University Grants Commission, Government of India and the Gus R. Douglass Institute (graduate research assistantship to AV and BD). DC was supported by the Agricultural Genome Center of Next-Generation Biogreen 21 Program (PJ011275). NR 73 TC 3 Z9 3 U1 16 U2 16 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD NOV 3 PY 2016 VL 7 AR 1646 DI 10.3389/fpls.2016.01646 PG 12 WC Plant Sciences SC Plant Sciences GA EA8NB UT WOS:000386891000002 PM 27857720 ER PT J AU Hansen, ZR Everts, KL Fry, WE Gevens, AJ Grunwald, NJ Gugino, BK Johnson, DA Johnson, SB Judelson, HS Knaus, BJ McGrath, MT Myers, KL Ristaino, JB Roberts, PD Secor, GA Smart, CD AF Hansen, Zachariah R. Everts, Kathryne L. Fry, William E. Gevens, Amanda J. Gruenwald, Niklaus J. Gugino, Beth K. Johnson, Dennis A. Johnson, Steven B. Judelson, Howard S. Knaus, Brian J. McGrath, Margaret T. Myers, Kevin L. Ristaino, Jean B. Roberts, Pamela D. Secor, Gary A. Smart, Christine D. TI Genetic Variation within Clonal Lineages of Phytophthora infestans Revealed through Genotyping-By-Sequencing, and Implications for Late Blight Epidemiology SO PLOS ONE LA English DT Article ID IRISH POTATO FAMINE; NEIGHBOR-JOINING METHOD; EASTERN UNITED-STATES; SEXUAL REPRODUCTION; R-PACKAGE; POPULATIONS; TOMATO; DIVERSITY; MARKERS; AGGRESSIVENESS AB Genotyping-by-sequencing (GBS) was performed on 257 Phytophthora infestans isolates belonging to four clonal lineages to study within-lineage diversity. The four lineages used in the study were US-8 (n = 28), US-11 (n = 27), US-23 (n = 166), and US-24 (n = 36), with isolates originating from 23 of the United States and Ontario, Canada. The majority of isolates were collected between 2010 and 2014 (94%), with the remaining isolates collected from 1994 to 2009, and 2015. Between 3,774 and 5,070 single-nucleotide polymorphisms (SNPs) were identified within each lineage and were used to investigate relationships among individuals. K-means hierarchical clustering revealed three clusters within lineage US-23, with US-23 isolates clustering more by collection year than by geographic origin. K-means hierarchical clustering did not reveal significant clustering within the smaller US-8, US-11, and US-24 data sets. Neighbor-joining (NJ) trees were also constructed for each lineage. All four NJ trees revealed evidence for pathogen dispersal and overwintering within regions, as well as long-distance pathogen transport across regions. In the US-23 NJ tree, grouping by year was more prominent than grouping by region, which indicates the importance of long-distance pathogen transport as a source of initial late blight inoculum. Our results support previous studies that found significant genetic diversity within clonal lineages of P. infestans and show that GBS offers sufficiently high resolution to detect sub-structuring within clonal populations. C1 [Hansen, Zachariah R.; Smart, Christine D.] Cornell Univ, Plant Pathol & Plant Microbe Biol Sect, Geneva, NY 14456 USA. [Everts, Kathryne L.] Univ Maryland, Dept Plant Sci & Landscape Architecture, Salisbury, MD USA. [Fry, William E.; Myers, Kevin L.] Cornell Univ, Plant Pathol & Plant Microbe Biol Sect, Ithaca, NY USA. [Gevens, Amanda J.] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA. [Gruenwald, Niklaus J.; Knaus, Brian J.] ARS, Hort Crops Res Lab, USDA, Corvallis, OR USA. [Gugino, Beth K.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. [Johnson, Dennis A.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Johnson, Steven B.] Univ Maine Cooperat Extens, Presque Isle, ME USA. [Judelson, Howard S.] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. [McGrath, Margaret T.] Cornell Univ, Plant Pathol & Plant Microbe Biol Sect, Riverhead, NY USA. [Ristaino, Jean B.] North Carolina State Univ, Dept Plant Pathol, Raleigh, NC USA. [Roberts, Pamela D.] Univ Florida, Dept Plant Pathol, Immokalee, FL USA. [Secor, Gary A.] North Dakota State Univ, Dept Plant Pathol, Fargo, ND USA. RP Smart, CD (reprint author), Cornell Univ, Plant Pathol & Plant Microbe Biol Sect, Geneva, NY 14456 USA. EM cds14@cornell.edu OI Ristaino, Jean/0000-0002-9458-0514 FU United States Department of Agriculture, National Institute of Food and Agriculture [2011-68004-30154]; United States Department of Agriculture, National Institute of Food and Agriculture Pre-Doctoral Fellowship [2016-67011-25176] FX Funding for this work was provided by the United States Department of Agriculture, National Institute of Food and Agriculture Grant no. 2011-68004-30154. Additional support for Z. R. Hansen was provided by the United States Department of Agriculture, National Institute of Food and Agriculture Pre-Doctoral Fellowship no. 2016-67011-25176. NR 55 TC 0 Z9 0 U1 17 U2 17 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 3 PY 2016 VL 11 IS 11 AR e0165690 DI 10.1371/journal.pone.0165690 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EA8SX UT WOS:000386910000048 PM 27812174 ER PT J AU Niedz, RP AF Niedz, Randall P. TI ARS-Media for Excel: A Spreadsheet Tool for Calculating Media Recipes Based on Ion-Specific Constraints SO PLOS ONE LA English DT Article ID TISSUE-CULTURE MEDIUM; IN-VITRO CULTURE; MINERAL-COMPOSITION; OLIVE TREES; POTASSIUM; GROWTH; SODIUM; MICROPROPAGATION; CULTIVARS; NUTRITION AB ARS-Media for Excel is an ion solution calculator that uses "Microsoft Excel" to generate recipes of salts for complex ion mixtures specified by the user. Generating salt combinations (recipes) that result in pre-specified target ion values is a linear programming problem. Excel's Solver add-on solves the linear programming equation to generate a recipe. Calculating a mixture of salts to generate exact solutions of complex ionic mixtures is required for at least 2 types of problems-1) formulating relevant ecological/biological ionic solutions such as those from a specific lake, soil, cell, tissue, or organ and, 2) designing ion confounding-free experiments to determine ion-specific effects where ions are treated as statistical factors. Using ARS-Media for Excel to solve these two problems is illustrated by 1) exactly reconstructing a soil solution representative of a loamy agricultural soil and, 2) constructing an ion-based experiment to determine the effects of substituting Na+ for K+ on the growth of a Valencia sweet orange nonembryogenic cell line. C1 [Niedz, Randall P.] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP Niedz, RP (reprint author), ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. EM randall.niedz@ars.usda.gov NR 22 TC 0 Z9 0 U1 1 U2 1 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 3 PY 2016 VL 11 IS 11 AR e0166025 DI 10.1371/\ournal.pone.0166025 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EA8SX UT WOS:000386910000095 PM 27812202 ER PT J AU Makkonen, J Vesterbacka, A Martin, F Jussila, J Dieguez-Uribeondo, J Kortet, R Kokko, H AF Makkonen, Jenny Vesterbacka, Arto Martin, Frank Jussila, Japo Dieguez-Uribeondo, Javier Kortet, Raine Kokko, Harri TI Mitochondrial genomes and comparative genomics of Aphanomyces astaci and Aphanomyces invadans SO SCIENTIFIC REPORTS LA English DT Article ID EPIZOOTIC ULCERATIVE SYNDROME; IRISH POTATO FAMINE; FRESH-WATER CRAYFISH; PHYTOPHTHORA-INFESTANS; INVERTED REPEAT; PHYLOGENETIC-RELATIONSHIPS; RNA GENES; DNA; PATHOGEN; SEQUENCES AB The genus Aphanomyces (Saprolegniales, Oomycetes) includes species with a variety of ecologies from saprotrophs to plant and animal parasites. Two important species in this genus are A. astaci, the cause of crayfish plague and its close relative, A. invadans, which causes the epizootic ulcerative syndrome on fish. In this study, we have assembled and annotated the mitochondrial (mt) genomes of A. astaci and A. invadans from the whole genome shotgun sequence reads (PRJNA187372; PRJNA258292, respectively). The assembly was generated from A. astaci Pc-genotype strain APO3 and A. invadans strain NJM9701. The sizes of the mtDNAs were 49,489 bp and 49,061 bp for A. astaci and A. invadans, respectively. The species shared similar genetic content and organization encoding 35 proteins, two ribosomal RNAs, three putative open reading frames and 33 transfer RNAs of 19 amino acids for peptide synthesis. Both species also had a large inverted repeat region (LIR) of approximately 12 kb, the LIR contained large and small ribosomal RNAs and eight protein coding genes. These annotated mt genomes serve as a valuable genetic backbone for further development of diagnostic methods and phylogenetic and migration studies of the animal parasitic species of Aphanomyces. C1 [Makkonen, Jenny; Vesterbacka, Arto; Jussila, Japo; Kokko, Harri] Univ Eastern Finland, Dept Environm & Biol Sci, POB 1627, FI-70211 Kuopio, Finland. [Martin, Frank] ARS, USDA, 1636 E Alisal St, Salinas, CA 93905 USA. [Dieguez-Uribeondo, Javier] CSIC, Real Jardin Bot, Plaza Murillo 2, E-28014 Madrid, Spain. [Kortet, Raine] Univ Eastern Finland, Dept Environm & Biol Sci, POB 111, FI-80101 Joensuu, Finland. [Kortet, Raine] Univ Western Ontario, Dept Biol Biol & Geol Sci, 1151 Richmond St N, London, ON N6A 5B7, Canada. RP Makkonen, J (reprint author), Univ Eastern Finland, Dept Environm & Biol Sci, POB 1627, FI-70211 Kuopio, Finland. EM jenny.makkonen@uef.fi RI Kortet, Raine/J-5027-2012; OI Kortet, Raine/0000-0003-3749-1096; Makkonen, Jenny/0000-0002-5425-0334 FU University of Eastern Finland (Innovative Research Initiatives); LIFE+ CrayMate [LIFE12 INF/FI/233]; Finnish Cultural Foundation; Maj and Tor Nessling Foundation; Ministerio de Economia y Competitividad, Spain [CGL2012-39357] FX This research has been supported by the strategic funding of the University of Eastern Finland (Innovative Research Initiatives) (HK, AV, JM), LIFE+ CrayMate (JJ) (LIFE12 INF/FI/233), Finnish Cultural Foundation (RK) and Maj and Tor Nessling Foundation (JJ, JM). JD-U was supported by project of the Ministerio de Economia y Competitividad, Spain (CGL2012-39357). NR 60 TC 0 Z9 0 U1 11 U2 11 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD NOV 3 PY 2016 VL 6 AR 36089 DI 10.1038/srep36089 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EA7XT UT WOS:000386846900001 PM 27808238 ER PT J AU Ji, HR Chen, LH Zhu, JY Gleisner, R Zhang, X AF Ji, Hairui Chen, Liheng Zhu, J. Y. Gleisner, Roland Zhang, X. TI Reaction Kinetics Based Optimization of Furfural Production from Corncob Using a Fully Recyclable Solid Acid SO INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH LA English DT Article ID COMBINED HYDROLYSIS FACTOR; HIGH-YIELD CONVERSION; LEVULINIC ACID; DILUTE-ACID; CATALYZED-HYDROLYSIS; ETHANOL-PRODUCTION; LODGEPOLE PINE; HIGH-TITER; CELLULOSE; BIOCONVERSION AB To achieve green processing through recycling catalysts, this study demonstrated a commercial solid acid toluenesulfonic acid (p-TsOH) for furfural production from corncob. Acid recyclability study indicate that crystalized p-TsOH was highly effective for furfural production. A kinetic based reaction severity, combined hydrolysis factor (CHF), was used to develop a furfural predictive model with the consideration of loss through degradation. Furfural yield of approximately 75% theoretical was achieved in a CHF range between 830 and 1850. C1 [Ji, Hairui; Zhang, X.] Beijing Univ Chem Technol, Natl Energy R&D Ctr Biorefinery, Beijing 100029, Peoples R China. [Chen, Liheng] South China Univ Technol, Guangzhou 510641, Guangdong, Peoples R China. [Ji, Hairui; Chen, Liheng; Zhu, J. Y.; Gleisner, Roland] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Zhu, JY (reprint author), US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. EM jzhu@fs.fed.us OI Chen, Liheng/0000-0001-7501-7972 FU Chinese Scholarship Council (CSC) FX This work was conducted on official government time of J. Y. Zhu and R. Gleisner while H. Ji was a visiting student at the USDA Forest Service, Forest Products Laboratory (FPL). This work was supported by the Chinese Scholarship Council (CSC) that made the visiting appointment of H. Ji at the USDA Forest Products Laboratory (FPL) possible. We would like to acknowledge Fred Matt of the U.S. Forest Service, Forest Products Laboratory for conducting the carbohydrate analyses. NR 45 TC 0 Z9 0 U1 7 U2 7 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0888-5885 J9 IND ENG CHEM RES JI Ind. Eng. Chem. Res. PD NOV 2 PY 2016 VL 55 IS 43 BP 11253 EP 11259 DI 10.1021/acs.iecr.6b03243 PG 7 WC Engineering, Chemical SC Engineering GA EB1FN UT WOS:000387095100006 ER PT J AU Moore, GG Mack, BM Beltz, SB Gilbert, MK AF Moore, Geromy G. Mack, Brian M. Beltz, Shannon B. Gilbert, Matthew K. TI Draft Genome Sequence of an Aflatoxigenic Aspergillus Species, A. bombycis SO GENOME BIOLOGY AND EVOLUTION LA English DT Article DE Aspergillus bombycis; aflatoxins; genome sequence; phylogenomics; mating-type locus ID GENE CLUSTERS; BIOSYNTHESIS; METABOLITE; FLAVUS; IDENTIFICATION; AFLATREM; NOMIUS AB Aspergillus bombycis was first isolated from silkworm frass in Japan. It has been reportedly misidentified as A. nomius due to their macro-morphological and chemotype similarities. We sequenced the genome of the A. bombycis Type strain and found it to be comparable in size (37Mb), as well as in numbers of predicted genes (12,266), to other sequenced Aspergilli. The aflatoxin gene cluster in this strain is similar in size and the genes are oriented the same as other B-+G-aflatoxin producing species, and this strain contains a complete but nonfunctional gene cluster for the production of cyclopiazonic acid. Our findings also showed that the A. bombycis Type strain contains a single MAT1-2 gene indicating that this species is likely heterothallic (self-infertile). This draft genome will contribute to our understanding of the genes and pathways necessary for aflatoxin synthesis as well as the evolutionary relationships of aflatoxigenic fungi. C1 [Moore, Geromy G.; Mack, Brian M.; Beltz, Shannon B.; Gilbert, Matthew K.] ARS, Southern Reg Res Ctr, USDA, Washington, DC 20250 USA. RP Moore, GG (reprint author), ARS, Southern Reg Res Ctr, USDA, Washington, DC 20250 USA. EM geromy.moore@ars.usda.gov FU Agricultural Research Service, an agency within the United States Department of Agriculture FX This work was supported by research funding from the Agricultural Research Service, an agency within the United States Department of Agriculture. NR 23 TC 0 Z9 0 U1 1 U2 1 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1759-6653 J9 GENOME BIOL EVOL JI Genome Biol. Evol. PD NOV PY 2016 VL 8 IS 11 BP 3297 EP 3300 DI 10.1093/gbe/evw238 PG 4 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA EK3GU UT WOS:000393815300005 PM 27664179 ER PT J AU Niehaus, EM Munsterkotter, M Proctor, RH Brown, DW Sharon, A Idan, Y Oren-Young, L Sieber, CM Novak, O Pencik, A Tarkowska, D Hromadova, K Freeman, S Maymon, M Elazar, M Youssef, SA El-Shabrawy, EM Shalaby, ABA Houterman, P Brock, NL Burkhardt, I Tsavkelova, EA Dickschat, JS Galuszka, P Guldener, U Tudzynski, B AF Niehaus, Eva-Maria Muensterkoetter, Martin Proctor, Robert H. Brown, Daren W. Sharon, Amir Idan, Yifat Oren-Young, Liat Sieber, Christian M. Novak, Ondrej Pencik, Ales Tarkowska, Danuse Hromadova, Kristyna Freeman, Stanley Maymon, Marcel Elazar, Meirav Youssef, Sahar A. El-Shabrawy, El Said M. Shalaby, Abdel Baset A. Houterman, Petra Brock, Nelson L. Burkhardt, Immo Tsavkelova, Elena A. Dickschat, Jeroen S. Galuszka, Petr Gueldener, Ulrich Tudzynski, Bettina TI Comparative "Omics" of the Fusarium fujikuroi Species Complex Highlights Differences in Genetic Potential and Metabolite Synthesis SO GENOME BIOLOGY AND EVOLUTION LA English DT Article DE Fusarium fujikuroi species complex; genome sequencing; secondary metabolism; in planta expression; metabolomics; evolution ID FUNCTIONAL-CHARACTERIZATION; GIBBERELLA-FUJIKUROI; NATURAL-PRODUCTS; RNA-SEQ; TRANSCRIPTION FACTORS; BIOSYNTHETIC-PATHWAY; POLYKETIDE SYNTHASES; AUXIN BIOSYNTHESIS; MANGO MALFORMATION; ACID AB Species of the Fusarium fujikuroi species complex (FFC) cause a wide spectrum of often devastating diseases on diverse agricultural crops, including coffee, fig, mango, maize, rice, and sugarcane. Although species within the FFC are difficult to distinguish by morphology, and their genes often share 90% sequence similarity, they can differ in host plant specificity and life style. FFC species can also produce structurally diverse secondary metabolites (SMs), including the mycotoxins fumonisins, fusarins, fusaric acid, and beauvericin, and the phytohormones gibberellins, auxins, and cytokinins. The spectrum of SMs produced can differ among closely related species, suggesting that SMs might be determinants of host specificity. To date, genomes of only a limited number of FFC species have been sequenced. Here, we provide draft genome sequences of three more members of the FFC: a single isolate of F. mangiferae, the cause of mango malformation, and two isolates of F. proliferatum, one a pathogen of maize and the other an orchidendophyte. We compared these genomes to publicly available genome sequences of three other FFC species. The comparisons revealed species-specific and isolate-specific differences in the composition and expression (in vitro and in planta) of genes involved in SM production including those for phytohormome biosynthesis. Such differences have the potential to impact host specificity and, as in the case of F. proliferatum, the pathogenic versus endophytic life style. C1 [Niehaus, Eva-Maria; Tudzynski, Bettina] Westfal Wilhelms Univ Munster, Mol Biol & Biotechnol Fungi, Inst Biol & Biotechnol Pflanzen, Munster, Germany. [Muensterkoetter, Martin] German Res Ctr Environm Hlth GmbH, Helmholtz Zentrum Munchen, Inst Bioinformat & Syst Biol, Neuherberg, Germany. [Proctor, Robert H.; Brown, Daren W.] USDA, Natl Ctr Agr Utilizat Res, Peoria, IL USA. [Sharon, Amir; Idan, Yifat; Oren-Young, Liat] Tel Aviv Univ, Dept Mol Biol & Ecol Plants, Tel Aviv, Israel. [Sieber, Christian M.] Univ Calif, Dept Energy, Joint Genome Inst, Berkeley, CA USA. [Novak, Ondrej; Pencik, Ales; Tarkowska, Danuse; Hromadova, Kristyna; Galuszka, Petr] Palacky Univ, Ctr Reg Hana Biotechnol & Agr Res, Olomouc, Czech Republic. [Freeman, Stanley; Maymon, Marcel; Elazar, Meirav] Agr Res Org, Volcani Ctr, Dept Plant Pathol & Weed Res, Bet Dagan, Israel. [Youssef, Sahar A.; El-Shabrawy, El Said M.; Shalaby, Abdel Baset A.] Agr Res Ctr, Plant Pathol Res Inst, Giza, Egypt. [Houterman, Petra] Univ Amsterdam, Swammerdam Inst Life Sci, Plant Pathol, Amsterdam, Netherlands. [Brock, Nelson L.; Burkhardt, Immo; Dickschat, Jeroen S.] Rhein Friedrich Wilhelms Univ Bonn, Kekule Inst Organ Chem & Biochem, Bonn, Germany. [Tsavkelova, Elena A.] Lomonosov Moscow State Univ, Fac Biol, Dept Microbiol, Moscow, Russia. [Gueldener, Ulrich] Tech Univ Munich, Wissenschaftszentrum Weihenstephan, Dept Genome Oriented Bioinformat, Maximus von Imhof Forum 3, Freising Weihenstephan, Germany. RP Tudzynski, B (reprint author), Westfal Wilhelms Univ Munster, Mol Biol & Biotechnol Fungi, Inst Biol & Biotechnol Pflanzen, Munster, Germany.; Guldener, U (reprint author), Tech Univ Munich, Wissenschaftszentrum Weihenstephan, Dept Genome Oriented Bioinformat, Maximus von Imhof Forum 3, Freising Weihenstephan, Germany. EM u.gueldener@tum.de; tudzynsb@uni-muenster.de OI Guldener, Ulrich/0000-0001-5052-8610 FU Deutsche Forschungsgemeinschaft (DFG) [TU101/17-2, GU1205/2-2]; Austrian Science Fund FWF [F3705/DFG ME1682/6-1]; National Science Foundation, Czech Republic [16-10602S] FX This study was supported by the Deutsche Forschungsgemeinschaft (DFG), projects TU101/17-2 and GU1205/2-2, by the Austrian Science Fund FWF (special research project Fusarium, F3705/DFG ME1682/6-1) and the National Science Foundation, Czech Republic (grant number 16-10602S). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. We are grateful for the technical assistance of Stephanie Folmar, Marcie Moore and Crystal Probyn. We thank Martijn Rep (University of Amsterdam, The Netherlands) for support in performing CHEF gel analysis. We thank also Jose J. Espino for transforming Fusarium strains. NR 125 TC 1 Z9 1 U1 2 U2 2 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1759-6653 J9 GENOME BIOL EVOL JI Genome Biol. Evol. PD NOV PY 2016 VL 8 IS 11 BP 3574 EP 3599 DI 10.1093/gbe/evw259 PG 26 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA EK3GU UT WOS:000393815300022 PM 28040774 ER PT J AU Hibbett, D Abarenkov, K Koljalg, U Opik, M Chai, B Cole, J Wang, Q Crous, P Robert, V Helgason, T Herr, JR Kirk, P Lueschow, S O'Donnell, K Nilsson, RH Oono, R Schoch, C Smyth, C Walker, DM Porras-Alfaro, A Taylor, JW Geiser, DM AF Hibbett, David Abarenkov, Kessy Koljalg, Urmas Opik, Maarja Chai, Benli Cole, James Wang, Qiong Crous, Pedro Robert, Vincent Helgason, Thorunn Herr, Joshua R. Kirk, Paul Lueschow, Shiloh O'Donnell, Kerry Nilsson, R. Henrik Oono, Ryoko Schoch, Conrad Smyth, Christopher Walker, Donald M. Porras-Alfaro, Andrea Taylor, John W. Geiser, David M. TI Sequence-based classification and identification of Fungi SO MYCOLOGIA LA English DT Review DE biodiversity informatics; metagenomics; molecular ecology; nomenclature; systematics; taxonomy ID INTERNAL TRANSCRIBED SPACER; RIBOSOMAL DATABASE PROJECT; ARBUSCULAR MYCORRHIZAL FUNGI; OPERATIONAL TAXONOMIC UNITS; SPECIES LIVING TREE; RNA GENE DATABASE; AD-HOC-COMMITTEE; LARGE-SUBUNIT; MOLECULAR-IDENTIFICATION; ENVIRONMENTAL SEQUENCES AB Fungal taxonomy and ecology have been revolutionized by the application of molecular methods and both have increasing connections to genomics and functional biology. However, data streams from traditional specimen- and culture-based systematics are not yet fully integrated with those from metagenomic and metatranscriptomic studies, which limits understanding of the taxonomic diversity and metabolic properties of fungal communities. This article reviews current resources, needs, and opportunities for sequence-based classification and identification (SBCI) in fungi as well as related efforts in prokaryotes. To realize the full potential of fungal SBCI it will be necessary to make advances in multiple areas. Improvements in sequencing methods, including long-read and single-cell technologies, will empower fungal molecular ecologists to look beyond ITS and current shotgun metagenomics approaches. Data quality and accessibility will be enhanced by attention to data and metadata standards and rigorous enforcement of policies for deposition of data and workflows. Taxonomic communities will need to develop best practices for molecular characterization in their focal clades, while also contributing to globally useful datasets including ITS. Changes to nomenclatural rules are needed to enable valid publication of sequence-based taxon descriptions. Finally, cultural shifts are necessary to promote adoption of SBCI and to accord professional credit to individuals who contribute to community resources. C1 [Hibbett, David] Clark Univ, Dept Biol, Worcester, MA 01610 USA. [Abarenkov, Kessy; Koljalg, Urmas; Opik, Maarja] Univ Tartu, Inst Ecol & Earth Sci, 40 Lai St, EE-51005 Tartu, Estonia. [Chai, Benli; Cole, James; Wang, Qiong] Michigan State Univ, Dept Plant Soil & Microbial Sci, Plant & Soil Sci Bldg,1066 Bogue St,Room 540, E Lansing, MI 48824 USA. [Crous, Pedro; Robert, Vincent] Cent Bur Schimmelcultures Fungal Biodivers Ctr CB, NL-3508 AD Utrecht, Netherlands. [Helgason, Thorunn] Univ York, Dept Biol, York YO10 5DD, N Yorkshire, England. [Herr, Joshua R.] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68503 USA. [Herr, Joshua R.] Univ Nebraska, Ctr Plant Sci Innovat, Lincoln, NE 68503 USA. [Kirk, Paul] Royal Bot Gardens, Biodivers Informat & Spatial Anal, Richmond TW9 3AF, Surrey, England. [Lueschow, Shiloh; O'Donnell, Kerry] NCAUR ARS USDA, 1815 N Univ St, Peoria, IL 61604 USA. [Nilsson, R. Henrik] Univ Gothenburg, Dept Biol & Environm Sci, S-40530 Gothenburg, Sweden. [Oono, Ryoko] Univ Calif Santa Barbara, Dept Ecol Evolut & Marine Biol, Santa Barbara, CA 93106 USA. [Schoch, Conrad] NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20892 USA. [Smyth, Christopher; Geiser, David M.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, 121 Buckhout Lab, University Pk, PA 16802 USA. [Walker, Donald M.] Tennessee Technol Univ, Dept Biol, 1100 N Dixie Ave, Cookeville, TN 38505 USA. [Porras-Alfaro, Andrea] Western Illinois Univ, Dept Biol Sci, Waggoner Hall 372,1 Univ Circle, Macomb, IL 61455 USA. [Taylor, John W.] Univ Calif Berkeley, Dept Plant & Microbial Biol, 111 Koshland Hall, Berkeley, CA 94720 USA. RP Hibbett, D (reprint author), Clark Univ, Dept Biol, Worcester, MA 01610 USA. EM dhibbett@clarku.edu FU US National Science Foundation [DEB 1424740]; Intramural Research Program of the National Institutes of Health, National Library of Medicine; Estonian Research Council [IUT20-28]; European Regional Development Fund (Centre of Excellence EcolChange); NSF [DEB-1208719] FX Preparation of this article was initiated in a 2 d workshop on SBCI in Fungi that was held at the Kellogg Center of Michigan State University, East Lansing, Michigan, 12-13 Jun 2014 (Herr et al. 2015), which was supported by US National Science Foundation award No. DEB 1424740 to DG, AP-A, DSH, and JWT. A related symposium on "Sequence-based identification in Fungi" was held on 11 Jun 2014 at the annual meeting of the Mycological Society of America, and a Special Interest Group session on "Classifying, naming and communicating sequence based species" took place at the International Mycological Congress, 5 Aug 2014, Bangkok, Thailand. The authors thank Sam Kovaka, who performed the query of GenBank for species names (FIG. 2, SUPPLEMENTARY INFORMATION), P. Brandon Matheny, and two anonymous reviewers for helpful comments. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the Victorian government or the US Department of Agriculture. USDA is an equal opportunity provider and employer. CLS acknowledges support from the Intramural Research Program of the National Institutes of Health, National Library of Medicine. MO acknowledges support from the Estonian Research Council (grant No. IUT20-28) and European Regional Development Fund (Centre of Excellence EcolChange). DSH acknowledges support of NSF award No. DEB-1208719. NR 142 TC 0 Z9 0 U1 7 U2 7 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD NOV-DEC PY 2016 VL 108 IS 6 BP 1049 EP 1068 DI 10.3852/16-130 PG 20 WC Mycology SC Mycology GA EJ5PA UT WOS:000393269000001 ER PT J AU Hesse, CN Torres-Cruz, TJ Tobias, TB Al-Matruk, M Porras-Alfaro, A Kuske, CR AF Hesse, Cedar N. Torres-Cruz, Terry J. Tobias, Terri Billingsley Al-Matruk, Maryam Porras-Alfaro, Andrea Kuske, Cheryl R. TI Ribosomal RNA gene detection and targeted culture of novel nitrogen-responsive fungal taxa from temperate pine forest soil SO MYCOLOGIA LA English DT Article DE Duke Forest; Endogone; Mucoromycotina; Saccharomycotina; soil nitrogen ID ATMOSPHERIC CO2; HIGH-THROUGHPUT; LARGE-SUBUNIT; DIVERSITY; COMMUNITIES; BACTERIAL; CLASSIFICATION; IDENTIFICATION; ALIGNMENT; REVEALS AB Soil fungal communities are responsible for carbon and nitrogen (N) cycling. The high complexity of the soil fungal community and the high proportion of taxonomically unidentifiable sequences confound ecological interpretations in field studies because physiological information is lacking for many organisms known only by their rRNA sequences. This situation forces experimental comparisons to be made at broader taxonomic racks where functions become difficult to infer. The objective of this study was to determine OTU (operational taxonomic units) level responses of the soil fungal community to N enrichment in a temperate pine forest experiment and to use the sequencing data to guide culture efforts of novel N-responsive fungal taxa. Replicate samples from four soil horizons (up to 10 cm depth) were obtained from ambient, enriched CO2 and N-fertilization plots. Through a fungal large subunit rRNA gene (LSU) sequencing survey, we identified two novel fungal clades that were abundant in our soil sampling (representing up to 27% of the sequences in some samples) and responsive to changes in soil N. The two N-responsive taxa with no predicted taxonomic association were targeted for isolation and culturing from specific soil samples where their sequences were abundant. Representatives of both OTUs were successfully cultured using a filtration approach. One taxon (OTU6) was most closely related to Saccharomycotina; the second taxon (OTU69) was most closely related to Mucoromycotina. Both taxa likely represent novel species. This study shows how observation of specific OTUs level responses to altered N status in a large rRNA gene field survey provided the impetus to design targeted culture approaches for isolation of novel N-responsive fungal taxa. C1 [Hesse, Cedar N.; Kuske, Cheryl R.] Los Alamos Natl Lab, Biosci Div, Los Alamos, NM 87545 USA. [Torres-Cruz, Terry J.; Tobias, Terri Billingsley; Al-Matruk, Maryam; Porras-Alfaro, Andrea] Western Illinois Univ, Dept Biol Sci, Macomb, IL 61455 USA. [Hesse, Cedar N.] ARS, USDA, Corvallis, OR 97331 USA. RP Kuske, CR (reprint author), Los Alamos Natl Lab, Biosci Div, Los Alamos, NM 87545 USA. EM kuske@lanl.gov FU Science Focus Area grant from the US Department of Energy, Biological and Environmental Research Division; RISE (Research Inspiring Student Excellence) and Women in Science programs at Western Illinois University FX This study was supported through a Science Focus Area grant to CRK from the US Department of Energy, Biological and Environmental Research Division, and through the RISE (Research Inspiring Student Excellence) and Women in Science programs at Western Illinois University. NR 40 TC 0 Z9 0 U1 2 U2 2 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD NOV-DEC PY 2016 VL 108 IS 6 BP 1082 EP 1090 DI 10.3852/16-086 PG 9 WC Mycology SC Mycology GA EJ5PA UT WOS:000393269000003 ER PT J AU Kepler, RM Chen, Y Kilcrease, J Shao, J Rehner, SA AF Kepler, Ryan M. Chen, Yuan Kilcrease, James Shao, Jonathan Rehner, Stephen A. TI Independent origins of diploidy in Metarhizium SO MYCOLOGIA LA English DT Article DE hybridization; mating type; next-generation multilocus sequence typing (NGMLST); whole genome duplication (WGD) ID MAXIMUM-LIKELIHOOD; ANISOPLIAE; SEQUENCE; PATHOGEN; DIVERSITY; SOFTWARE; GENOTYPE; MODELS AB In fungi, stable diploid genome arrangements are rare. Here we present evidence from nuclear intergenic DNA sequencing, microsatellite genotyping, and configuration of the mating-type locus to demonstrate two independent origins of persistent diploid genome organization in the Metarhizium majus species complex. Most taxa in the complex are genotypically haploid, with individual isolates consistently displaying a single allele across all nuclear loci, as well as having a single mating-type locus. In contrast, individuals of M. majus and the Glade designated here MGT1 are shown to be diploid, based on a consistent finding of heterozygosity and the presence of both MAT1 and MAT2 mating-type loci. In single locus phylogenies, nuclear intergenic alleles of M. majus and MGT1 each form monophyletic groups, indicating that diploidy in both taxa likely originated by the union of conspecific individuals. Sequence divergence in the APN2/MAT1-1-3 and APN2/MAT2-1 intergenic spacers indicate the two MAT loci are physically separated in the genomes of both diploid taxa, although the linkage relationship of the MAT loci to one another is unknown. The presence of both mating genes in a single nucleus suggests these diploid genomes may represent a mating event that failed to complete meiosis. Whether or not these isolates are able to complete the sexual cycle under any conditions and fosse ascospores remains an open question. C1 [Kepler, Ryan M.; Rehner, Stephen A.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Chen, Yuan] Duke Univ, Med Ctr, Dept Med, Div Infect Dis, Durham, NC 20705 USA. [Kilcrease, James] USDA ARS, Floral & Nursery Plant Res Unit, Beltsville, MD 20705 USA. [Shao, Jonathan] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. RP Rehner, SA (reprint author), USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. EM stephen.rehner@ars.usda.gov NR 34 TC 0 Z9 0 U1 0 U2 0 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD NOV-DEC PY 2016 VL 108 IS 6 BP 1091 EP 1103 DI 10.3852/15-270 PG 13 WC Mycology SC Mycology GA EJ5PA UT WOS:000393269000004 ER PT J AU Grafenhan, T Johnston, PR Vaughan, MM McCormick, SP Proctor, RH Busman, M Ward, TJ O'Donnell, K AF Grafenhan, Tom Johnston, Peter R. Vaughan, Martha M. McCormick, Susan P. Proctor, Robert H. Busman, Mark Ward, Todd J. O'Donnell, Kerry TI Fusarium praegraminearum sp nov., a novel nivalenol mycotoxin-producing pathogen from New Zealand can induce head blight on wheat SO MYCOLOGIA LA English DT Article DE genotyping; morphology; pathogenicity; phylogenetics; RPB1; RPB2; trichothecene; zearalenone ID TRICHOTHECENE MYCOTOXIN; GRAMINEARUM; DEOXYNIVALENOL; POLYMORPHISM; POPULATIONS; COMPLEX; CEREAL; FUNGI AB We report on the molecular and morphological characterization of a novel type B trichothecene toxin-producing species (i.e. B Glade) recovered from litter in a maize field near Wellington, New Zealand, which is described as Fusarium praegraminearum sp. nov. This species was initially identified as F. acuminatum based on morphological characters. However, it differs from this species by producing longer, slightly asymmetrically curved macroconidia in which the apical cell is not as pointed and by its much faster colony growth rate on agar. Molecular phylogenetic analyses of portions of 13 genes resolved F. praegraminearum as the most basal species within the B Glade. Mycotoxin analyses demonstrated that it was able to produce 4-acetylnivalenol and 4,15-diacetylnivalenol trichothecenes, the nontrichothecene sesquiterpenes culmorin and hydroxy-culmorins, and the estrogen zearalenone in vitro. Results of a pathogenicity experiment revealed that F. praegraminearum induced moderate head blight on wheat. C1 [Grafenhan, Tom] Canadian Grain Commiss, Grain Res Lab, Winnipeg, MB R3C 3G8, Canada. [Johnston, Peter R.] Landcare Res Manaaki Whenua, Private Bag 92170, Auckland 1142, New Zealand. [Vaughan, Martha M.; McCormick, Susan P.; Proctor, Robert H.; Busman, Mark; Ward, Todd J.; O'Donnell, Kerry] ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP O'Donnell, K (reprint author), ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. EM kerry.odonnell@ars.usda.gov NR 35 TC 0 Z9 0 U1 1 U2 1 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD NOV-DEC PY 2016 VL 108 IS 6 BP 1229 EP 1239 DI 10.3852/16-110 PG 11 WC Mycology SC Mycology GA EJ5PA UT WOS:000393269000013 ER PT J AU Miernyk, JA AF Miernyk, Jan A. TI Getting into mitochondria SO BIOCHEMICAL JOURNAL LA English DT Editorial Material ID TARGETING PEPTIDES; PROTEIN IMPORT; PROCESSING PEPTIDASE; CLEAVAGE-SITE; PRESEQUENCE; SEQUENCES; MOTIFS; DOMAIN; TRANSLOCATION; RECOGNITION AB The human mitochondrial glutamate dehydrogenase isoenzymes (hGDH1 and hGDH2) are abundant matrix-localized proteins encoded by nuclear genes. The proteins are synthesized in the cytoplasm, with an atypically long N-terminal mitochondrial targeting sequence (MTS). The results of secondary structure predictions suggest the presence of two alpha-helices within the N-terminal region of the MTS. Results from deletion analyses indicate that individual helices have limited ability to direct protein import and matrix localization, but that there is a synergistic interaction when both helices are present [Biochem. J. (2016) 473, 2813-2829]. Mutagenesis of the MTS cleavage sites blocked post-import removal of the presequences, but did not impede import. The authors propose that the high matrix levels of hGDH can be attributed to the unusual length and secondary structure of the MTS. C1 [Miernyk, Jan A.] ARS, USDA, Plant Genet Res Unit, Columbia, MO 65202 USA. [Miernyk, Jan A.] Univ Missouri, Div Biochem, Columbia, MO 65211 USA. RP Miernyk, JA (reprint author), ARS, USDA, Plant Genet Res Unit, Columbia, MO 65202 USA.; Miernyk, JA (reprint author), Univ Missouri, Div Biochem, Columbia, MO 65211 USA. EM miernykj@missouri.edu NR 36 TC 0 Z9 0 U1 1 U2 1 PU PORTLAND PRESS LTD PI LONDON PA CHARLES DARWIN HOUSE, 12 ROGER STREET, LONDON WC1N 2JU, ENGLAND SN 0264-6021 EI 1470-8728 J9 BIOCHEM J JI Biochem. J. PD NOV PY 2016 VL 473 BP 3755 EP 3758 DI 10.1042/BCJ20160667C PN 21 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EK2LG UT WOS:000393758200003 PM 27789742 ER PT J AU Puhlick, JJ Weiskittel, AR Fernandez, IJ Fraver, S Kenefic, LS Seymour, RS Kolka, RK Rustad, LE Brissette, JC AF Puhlick, Joshua J. Weiskittel, Aaron R. Fernandez, Ivan J. Fraver, Shawn Kenefic, Laura S. Seymour, Robert S. Kolka, Randall K. Rustad, Lindsey E. Brissette, John C. TI Long-term influence of alternative forest management treatments on total ecosystem and wood product carbon storage SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE carbon stocks; forest products; forest soils; coarse fragments; site quality ID MIXED-SPECIES STANDS; CENTRAL MAINE; USA; DYNAMICS; POOLS; CONIFERS; IMPACTS; STATES; PINE AB Developing strategies for reducing atmospheric CO2 is one of the foremost challenges facing natural resource professionals today. The goal of this study was to evaluate total ecosystem and harvested wood product carbon (C) stocks among alternative forest management treatments (selection cutting, shelterwood cutting, commercial clearcutting, and no management) in mixed-species stands in central Maine, USA. These treatments were initiated in the 1950s and have been maintained since, and ecosystem C pools were measured in 2012. When compared across managed treatments, the commercial clearcut had the lowest total ecosystem C stocks by 21%, on average (P < 0.05), while the selection and shelterwood treatments had similar total ecosystem C stocks. Including the C stored in harvested wood products did not influence observed differences in C storage among treatments. Total ecosystem C stocks in the reference stand were 247.0 +/- 17.7 Mg.ha(-1) (mean +/- SD) compared with 161.7 +/- 31.3 Mg.ha(-1) in the managed stands (171.2 +/- 31.7 Mg.ha(-1) with products C). This study highlights the impacts of long-term forest management treatments on C storage and indicates that the timing of harvests and the species and sizes of trees removed influence C stored in harvested wood products. C1 [Puhlick, Joshua J.; Weiskittel, Aaron R.; Fernandez, Ivan J.; Fraver, Shawn; Seymour, Robert S.] Univ Maine, Sch Forest Resources, Orono, ME 04469 USA. [Kenefic, Laura S.] US Forest Serv, USDA, Northern Res Stn, 686 Govt Rd, Bradley, ME 04411 USA. [Kolka, Randall K.] US Forest Serv, USDA, Northern Res Stn, 1831 Hwy 169 E, Grand Rapids, MN 55744 USA. [Rustad, Lindsey E.; Brissette, John C.] US Forest Serv, USDA, Northern Res Stn, 271 Mast Rd, Durham, NH 03824 USA. RP Puhlick, JJ (reprint author), Univ Maine, Sch Forest Resources, Orono, ME 04469 USA. EM joshua.puhlick@maine.edu FU USDA Forest Service, Northern Research Station; USDA Forest Service, Northeastern States Research Cooperative; Maine Agricultural and Forest Experiment Station; Penobscot Experimental Forest Research Fund FX We thank Christopher Woodall (USDA Forest Service), Alex Finkral (The Forestland Group), and two anonymous reviewers who provided useful comments that helped us to improve this manuscript. We acknowledge Bruce Hoskins, Sue Erich, and Cheryl Spencer (University of Maine) for their advice on soil sampling and laboratory procedures. Funding for this project was provided by the USDA Forest Service, Northern Research Station and Northeastern States Research Cooperative; the Maine Agricultural and Forest Experiment Station; and the Penobscot Experimental Forest Research Fund. NR 50 TC 0 Z9 0 U1 9 U2 9 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD NOV PY 2016 VL 46 IS 11 BP 1404 EP 1412 DI 10.1139/cjfr-2016-0193 PG 9 WC Forestry SC Forestry GA EJ3JJ UT WOS:000393108100018 ER PT J AU Galyean, ML Gunter, SA AF Galyean, M. L. Gunter, S. A. TI Predicting forage intake in extensive grazing systems SO JOURNAL OF ANIMAL SCIENCE LA English DT Article; Proceedings Paper CT 5th Grazing Livestock Nutrition Conference (GLNC) - Enhancing Management, Production, and Sustainability of Grazing Ruminants in Extensive Landscapes CY JUL 17-19, 2016 CL Park City, UT SP Amer Soc Anim Sci, Western Sect, Micronutrients, Novus, Montana State Univ, Agr Expt Stat, Oregon State Univ DE cattle; empirical models; forages; grazing; voluntary intake ID DRY-MATTER INTAKE; PLANT SECONDARY METABOLITES; MIDGRASS PRAIRIE RANGELAND; PLAINS BLUESTEM PASTURE; NET ENERGY-REQUIREMENTS; FEED-INTAKE REGULATION; FINISHING BEEF-CATTLE; FLAVORED WHEAT-STRAW; VOLUNTARY INTAKE; INTRARUMINAL INFUSIONS AB Voluntary intake by cattle and other ruminants is controlled by a complex mix of physical and physiological factors that interact with a variety of environmental, geo-spatial, and experiential influences external to the animal. These factors are intensified in grazing ruminants, where selective grazing and potential variability in dietary options also affect eating decisions. As a result of the complexity of intake control and associated interacting factors, developing methods that yield accurate and precise predictions of voluntary intake by grazing cattle has been a long-standing challenge for animal scientists. Nonetheless, reliable estimates of intake are necessary to make informed management decisions related to sustainable management of grazing lands and to provide economically sustainable quantities of supplemental nutrients to maintain desired production levels. Currently available empirical regression equations to predict intake include independent variables like BW and energy concentration (or digestibility). Adding production variables (e.g., ADG for growing cattle or calf ADG/weaning weight for cows) seems to improve the accuracy and precision of these regression models, but to be applied in practice, these production variables must be estimated from historical data, which adds another source of variation and could decrease the predictive ability of these equations. Similarly, intake can be predicted from estimates of energy requirements and energy concentration of the diet selected (the DMI required approach), but this method also involves forecasting of requirements. For all empirical methods, estimates of forage digestibility or energy concentration are essential, but obtaining accurate estimates is difficult with grazing livestock. More complex mechanistic or quasi-mechanistic models have been developed, but the application of these models has been too limited to determine whether they offer significant advantages over traditional empirical models. Because our knowledge base of how, in a quantitative sense, both intake control mechanisms and external factors influence voluntary intake by grazing ruminants is limited, development of tools for predicting intake is likely to be a long-term process. C1 [Galyean, M. L.] Texas Tech Univ, Coll Agr Sci & Nat Resources, Lubbock, TX 79409 USA. [Gunter, S. A.] USDA ARS, Southern Plains Range Res Stn, Woodward, OK 73801 USA. RP Galyean, ML (reprint author), Texas Tech Univ, Coll Agr Sci & Nat Resources, Lubbock, TX 79409 USA. EM michael.galyean@ttu.edu NR 150 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD NOV PY 2016 VL 94 SU 6 BP 26 EP 43 DI 10.2527/jas2016-0523 PG 18 WC Agriculture, Dairy & Animal Science SC Agriculture GA EI9MJ UT WOS:000392831900004 ER PT J AU Mulliniks, JT Cope, ER McFarlane, ZD Hobbs, JD Waterman, RC AF Mulliniks, J. T. Cope, E. R. McFarlane, Z. D. Hobbs, J. D. Waterman, R. C. TI Drivers of grazing livestock efficiency: how physiology, metabolism, experience and adaptability influence productivity SO JOURNAL OF ANIMAL SCIENCE LA English DT Article; Proceedings Paper CT 5th Grazing Livestock Nutrition Conference (GLNC) - Enhancing Management, Production, and Sustainability of Grazing Ruminants in Extensive Landscapes CY JUL 17-19, 2016 CL Park City, UT SP Amer Soc Anim Sci, Western Sect, Micronutrients, Novus, Montana State Univ, Agr Expt Stat, Oregon State Univ DE beef cow; efficiency; metabolic flexibility; rangelands ID SKELETAL-MUSCLE; DAIRY-COWS; BEEF-COWS; ENDURANCE EXERCISE; HEREFORD CATTLE; BODY CONDITION; RANGE; STRESS; ADAPTATIONS; ENVIRONMENT AB Beef cow efficiency is a century's old debate on what the criteria, certain phenotypic traits, and definition of an "efficient" cow. However, we do know that energy utilization by the cow herd is proportionally large compared to the rest of the sector, which accounts up to 70 to 75% of dietary energy for maintenance with the residual used for pregnancy, lactation, activity and adaptation to the environment. Therefore, leveraging genetic variation in cow energy efficiency by selecting cows that require less energy for maintenance potentially reduces total energy utilization for beef cattle production, which will ultimately improve production efficiency and profitability. For livestock producers, optimizing both economic and biological efficiency is critical. The continued viability of production systems utilizing rangelands requires more rapid adoption of innovative management practices and selection tools that lend to increased profitability through optimization of nutrient utilization and increased reproductive performance. However, the implementation of a gold standard to identify energy efficient beef cows has not yet been fully realized for the beef industry. Rangeland beef cow herds are required to be biologically and reproductively efficient in an array of ever changing environmental conditions. The most efficient cows are those that easily adapt to environmental changes and have the ability to conserve or reset their maintenance requirements to match current environmental conditions. To achieve this metabolic adaptability or flexibility, beef cows need to be suited to their environment and have the ability to acclimate to environmental changes. The purpose of this proceedings paper is to identify and describe factors that influence range cow efficiency and ultimately contributes to the sustainability (both from a productivity and economic view point) of range cows in extensive enterprises. C1 [Mulliniks, J. T.; Cope, E. R.; McFarlane, Z. D.; Hobbs, J. D.] Univ Tennessee, Dept Anim Sci, Knoxville, TN 37996 USA. [Waterman, R. C.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. RP Mulliniks, JT (reprint author), Univ Tennessee, Dept Anim Sci, Knoxville, TN 37996 USA. EM jmulli@utk.edu NR 54 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD NOV PY 2016 VL 94 SU 6 BP 111 EP 119 DI 10.2527/jas2015-0711 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA EI9MJ UT WOS:000392831900013 ER PT J AU Aoun, M Breiland, M Turner, MK Loladze, A Chao, S Xu, SS Ammar, K Anderson, JA Kolmer, JA Acevedo, M AF Aoun, Meriem Breiland, Matthew Turner, M. Kathryn Loladze, Alexander Chao, Shiaoman Xu, Steven S. Ammar, Karim Anderson, James A. Kolmer, James A. Acevedo, Maricelis TI Genome-Wide Association Mapping of Leaf Rust Response in a Durum Wheat Worldwide Germplasm Collection SO PLANT GENOME LA English DT Article ID F-SP TRITICI; PUCCINIA-RECONDITA-TRITICI; STRIPE RUST; RESISTANCE GENE; SPRING WHEAT; COMMON WHEAT; MOLECULAR CHARACTERIZATION; CHROMOSOME LOCATION; COMPLEMENTARY GENES; TETRAPLOID WHEAT AB Leaf rust (caused by Puccinia triticina Erikss. [Pt]) is increasingly impacting durum wheat (Triticum turgidum L. var. durum) production with the recent appearance of races with virulence to widely grown cultivars in many durum producing areas worldwide. A highly virulent P. triticina race on durum wheat was recently detected in Kansas. This race may spread to the northern Great Plains, where most of the US durum wheat is produced. The objective of this study was to identify sources of resistance to several races from the United States and Mexico at seedling stage in the greenhouse and at adult stage in field experiments. Genome-wide association study (GWAS) was used to identify single-nucleotide polymorphism (SNP) markers associated with leaf rust response in a worldwide durum wheat collection of 496 accessions. Thirteen accessions were resistant across all experiments. Association mapping revealed 88 significant SNPs associated with leaf rust response. Of these, 33 SNPs were located on chromosomes 2A and 2B, and 55 SNPs were distributed across all other chromosomes except for 1B and 7B. Twenty markers were associated with leaf rust response at seedling stage, while 68 markers were associated with leaf rust response at adult plant stage. The current study identified a total of 14 previously uncharacterized loci associated with leaf rust response in durum wheat. The discovery of these loci through association mapping (AM) is a significant step in identifying useful sources of resistance that can be used to broaden the relatively narrow leaf rust resistance spectrum in durum wheat germplasm. C1 [Aoun, Meriem; Breiland, Matthew; Acevedo, Maricelis] North Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Loladze, Alexander; Ammar, Karim] Int Maize & Wheat Improvement Ctr CIMMYT, Apdo Postal 6-641, Mexico City 06600, DF, Mexico. [Turner, M. Kathryn; Anderson, James A.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Chao, Shiaoman] USDA ARS, Genotyping Lab, Biosci Res Lab, Fargo, ND 58108 USA. [Xu, Steven S.] USDA ARS, Cereal Crops Res Unit, Northern Crop Sci Lab, Fargo, ND USA. [Kolmer, James A.] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Acevedo, M (reprint author), North Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. EM maricelis.acevedo@ndsu.edu FU NIFA, USDA FX SNP genotyping and phenotyping tests at the USDA-ARS Cereal Disease Laboratory and University of Minnesota were made possible by the Triticeae Coordinated Agricultural Project funded NIFA, USDA. NR 107 TC 0 Z9 0 U1 0 U2 0 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD NOV PY 2016 VL 9 IS 3 DI 10.3835/plantgenome2016.01.0008 PG 24 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA EJ3NX UT WOS:000393120000007 ER PT J AU Li, GQ Xu, XY Bai, GH Carver, BF Hunger, R Bonman, JM Kolmer, J Dong, HX AF Li, Genqiao Xu, Xiangyang Bai, Guihua Carver, Brett F. Hunger, Robert Bonman, J. Michael Kolmer, James Dong, Hongxu TI Genome-Wide Association Mapping Reveals Novel QTL for Seedling Leaf Rust Resistance in a Worldwide Collection of Winter Wheat SO PLANT GENOME LA English DT Article ID TRITICUM-AESTIVUM L.; LINKAGE DISEQUILIBRIUM; GENETIC DIVERSITY; HAPLOTYPE MAP; POPULATION-STRUCTURE; PUCCINIA-TRITICINA; MOLECULAR MARKER; AGRONOMIC TRAITS; HEXAPLOID WHEAT; MAIZE AB Leaf rust of wheat (Triticum aestivum L.) is a major disease that causes significant yield losses worldwide. The short-lived nature of leaf rust resistance (Lr) genes necessitates a continuous search for novel sources of resistance. We performed a genome-wide association study (GWAS) on a panel of 1596 wheat accessions. The panel was evaluated for leaf rust reaction by testing with a bulk of Puccinia triticina Eriks. (Pt) isolates collected from multiple fields of Oklahoma in 2013 and two predominant races in the fields of Oklahoma in 2015. The panel was genotyped with a set of 5011 single-nucleotide polymorphism (SNP) markers. A total of 14 quantitative trait loci (QTL) for leaf rust resistance were identified at a false discovery rate (FDR) of 0.01 using the mixed linear model (MLM). Of these, eight QTL reside in the vicinity of known genes or QTL, and more studies are needed to determine their relationship with known loci. QLr. stars-7AL1 is a new QTL to bread wheat but is close to a locus previously identified in durum wheat [Triticum turgidum L. subsp. durum (Desf.) Husn.]. The other five QTL, including QLr. stars-1BL3, QLr. stars-1DC1, QLr. stars-2BL1, QLr. stars-5BL1, and QLr. stars-7AS1, are likely novel loci for leaf rust resistance. The uneven distribution of the 14 QTL in the six subpopulations of the panel suggests that wheat breeders can enhance leaf rust resistance by selectively introgressing some of these QTL into their breeding materials. In addition, another 31 QTL were significantly associated with leaf rust resistance at a FDR of 0.05. C1 [Li, Genqiao; Xu, Xiangyang] USDA ARS, Wheat Peanut & Other Field Crops Res Unit, Stillwater, OK 74075 USA. [Bai, Guihua] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Li, Genqiao; Carver, Brett F.] Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74078 USA. [Hunger, Robert] Oklahoma State Univ, Entomol & Pathol Dept, Stillwater, OK 74078 USA. [Bonman, J. Michael] USDA ARS, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. [Kolmer, James] USDA ARS, Cereal Dis Lab, St Paul, MN 55106 USA. [Dong, Hongxu] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Dong, Hongxu] Univ Illinois, Energy Biosci Inst, Urbana, IL 61801 USA. RP Xu, XY (reprint author), USDA ARS, Wheat Peanut & Other Field Crops Res Unit, Stillwater, OK 74075 USA. EM xiangyang.xu@ars.usda.gov OI Dong, Hongxu/0000-0003-4028-3597 FU USDA National Institute of Food and Agriculture [2011-68002-30029] FX We thank Dr. Harold Bockelman of USDA-ARS National Small Grain Collection for providing germplasm used in this study, the National Research Initiative Competitive Grant 2011-68002-30029 (Triticeae-CAP) from the USDA National Institute of Food and Agriculture for providing SNP data, and Todd Lenger for his excellent technical support. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is equal-opportunity provider and employer. NR 69 TC 0 Z9 0 U1 5 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD NOV PY 2016 VL 9 IS 3 DI 10.3835/plantgenome2016.06.0051 PG 12 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA EJ3NX UT WOS:000393120000018 ER PT J AU Moghaddam, SM Mamidi, S Osorno, JM Lee, R Brick, M Kelly, J Miklas, P Urrea, C Song, QJ Cregan, P Grimwood, J Schmutz, J McClean, PE AF Moghaddam, Samira Mafi Mamidi, Sujan Osorno, Juan M. Lee, Rian Brick, Mark Kelly, James Miklas, Phillip Urrea, Carlos Song, Qijian Cregan, Perry Grimwood, Jane Schmutz, Jeremy McClean, Phillip E. TI Genome-Wide Association Study Identifies Candidate Loci Underlying Agronomic Traits in a Middle American Diversity Panel of Common Bean SO PLANT GENOME LA English DT Article ID PHASEOLUS-VULGARIS-L.; BRASSINOSTEROID SIGNAL-TRANSDUCTION; STEM-CELL MAINTENANCE; ARABIDOPSIS-THALIANA; LINKAGE DISEQUILIBRIUM; GENETIC DIVERSITY; POPULATION-STRUCTURE; MICROSATELLITE MARKERS; PLANT ARCHITECTURE; ASSAY DEVELOPMENT AB Common bean (Phaseolus vulgaris L.) breeding programs aim to improve both agronomic and seed characteristics traits. However, the genetic architecture of the many traits that affect common bean production are not completely understood. Genome-wide association studies (GWAS) provide an experimental approach to identify genomic regions where important candidate genes are located. A panel of 280 modern bean genotypes from race Mesoamerica, referred to as the Middle American Diversity Panel (MDP), were grown in four US locations, and a GWAS using >150,000 single-nucleotide polymorphisms (SNPs) (minor allele frequency [MAF] >= 5%) was conducted for six agronomic traits. The degree of inter-and intrachromosomal linkage disequilibrium (LD) was estimated after accounting for population structure and relatedness. The LD varied between chromosomes for the entire MDP and among race Mesoamerica and Durango-Jalisco genotypes within the panel. The LD patterns reflected the breeding history of common bean. Genome-wide association studies led to the discovery of new and known genomic regions affecting the agronomic traits at the entire population, race, and location levels. We observed strong colocalized signals in a narrow genomic interval for three interrelated traits: growth habit, lodging, and canopy height. Overall, this study detected similar to 30 candidate genes based on a priori and candidate gene search strategies centered on the 100-kb region surrounding a significant SNP. These results provide a framework from which further research can begin to understand the actual genes controlling important agronomic production traits in common bean. C1 [Moghaddam, Samira Mafi; Mamidi, Sujan; McClean, Phillip E.] North Dakota State Univ, Genom & Bioinformat Program, Fargo, ND 58105 USA. [Moghaddam, Samira Mafi; Mamidi, Sujan; Osorno, Juan M.; Lee, Rian; McClean, Phillip E.] North Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA. [Mamidi, Sujan; Grimwood, Jane; Schmutz, Jeremy] HudsonAlpha Inst Biotechnol, Huntsville, AL 35806 USA. [Brick, Mark] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Kelly, James] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Miklas, Phillip] USDA ARS, Vegetable & Forage Crops Res Unit, Prosser, WA 99350 USA. [Urrea, Carlos] Univ Nebraska, Dept Agron & Hort, Scottsbluff, NE 69361 USA. [Song, Qijian; Cregan, Perry] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. RP Moghaddam, SM (reprint author), North Dakota State Univ, Genom & Bioinformat Program, Fargo, ND 58105 USA.; Moghaddam, SM (reprint author), North Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA. EM samira.mm@gmail.com FU USDA, National Institute of Food and Agricultural (NIFA), Agriculture and Food Research Initiative (AFRI) [2009-01929] FX Financial support provided by USDA, National Institute of Food and Agricultural (NIFA), Agriculture and Food Research Initiative (AFRI), Project #2009-01929 NR 138 TC 0 Z9 0 U1 2 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD NOV PY 2016 VL 9 IS 3 DI 10.3835/plantgenome2016.02.0012 PG 21 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA EJ3NX UT WOS:000393120000009 ER PT J AU Talukder, ZI Seiler, GJ Song, QJ Ma, GJ Qi, LL AF Talukder, Zahirul I. Seiler, Gerald J. Song, Qijian Ma, Guojia Qi, Lili TI SNP Discovery and QTL Mapping of Sclerotinia Basal Stalk Rot Resistance in Sunflower using Genotyping-by-Sequencing SO PLANT GENOME LA English DT Article ID QUANTITATIVE TRAIT LOCI; HELIANTHUS-ANNUUS L.; COMPARATIVE GENETIC-ANALYSIS; DISEASE RESISTANCE; MOLECULAR MARKERS; COMPLEX TRAITS; LINKAGE MAPS; STEM ROT; POPULATIONS; GERMPLASMS AB Basal stalk rot (BSR), caused by the ascomycete fungus Sclerotinia sclerotiorum (Lib.) de Bary, is a serious disease of sunflower (Helianthus annuus L.) in the cool and humid production areas of the world. Quantitative trait loci (QTL) for BSR resistance were identified in a sunflower recombinant inbred line (RIL) population derived from the cross HA 441 x RHA 439. A genotyping-by-sequencing (GBS) approach was adapted to discover single nucleotide polymorphism (SNP) markers. A genetic linkage map was developed comprised of 1053 SNP markers on 17 linkage groups (LGs) spanning 1401.36 cM. The RILs were tested in five environments (locations and years) for resistance to BSR. Quantitative trait loci were identified in each environment separately and also with integrated data across environments. A total of six QTL were identified in all five environments: one of each on LGs 4, 9, 10, 11, 16, and 17. The most significant QTL, Qbsr-10.1 and Qbsr-17.1, were identified at multiple environments on LGs 10 and 17, explaining 31.6 and 20.2% of the observed phenotypic variance, respectively. The remaining four QTL, Qbsr-4.1, Qbsr-9.1, Qbsr-11.1, and Qbsr-16.1, were detected in only one environment on LGs 4, 9, 11, and 16, respectively. Each of these QTL explains between 6.4 and 10.5% of the observed phenotypic variation in the RIL population. Alleles conferring increased resistance were contributed by both parents. The potential of the Qbsr-10.1 and Qbsr-17.1 in marker-assisted selection (MAS) breeding are discussed. C1 [Talukder, Zahirul I.] North Dakota State Univ, Dept Plant Sci, 166 Loftsgard Hall, Fargo, ND 58102 USA. [Seiler, Gerald J.; Qi, Lili] USDA ARS, Sunflower & Plant Biol Res Unit, Northern Crop Sci Lab, 1307 18th St N, Fargo, ND 58102 USA. [Song, Qijian] USDA ARS, Soybean Genom & Improvement Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. [Ma, Guojia] North Dakota State Univ, Dept Plant Pathol, 306 Walster Hall, Fargo, ND 58102 USA. RP Qi, LL (reprint author), USDA ARS, Sunflower & Plant Biol Res Unit, Northern Crop Sci Lab, 1307 18th St N, Fargo, ND 58102 USA. EM lili.qi@ars.usda.gov FU USDA-ARS National Sclerotinia Initiative [5442-21220-028-00D]; USDA-ARS CRIS Project [5442-21000-039-00D] FX The authors would like to thank Dr. Loren Rieseberg for providing access to the Sunflower Genome Data Repository. We acknowledge the collaboration of Dr. Kevin McPhee and our retired colleague Dr. Tom Gulya during the early part of the project. We thank Drs. William Underwood and Shaobin Zhong for critically reviewing the manuscript. We are grateful to Dr. Yunming Long for his help in designing the allele-specific PCR primers and assay protocol. We also thank Angelia Hogness for her assistance in the lab and Chris Misar, Michelle Gilley, and Megan Ramsett for their assistance in the field. This research was supported by the USDA-ARS National Sclerotinia Initiative, grant number 5442-21220-028-00D and the USDA-ARS CRIS Project No. 5442-21000-039-00D. Mention of trade names or commercial products in this report is solely for the purpose of providing specific information and does not imply recommendations or endorsement by the USDA. The USDA is an equal opportunity provider and employer. NR 60 TC 0 Z9 0 U1 0 U2 0 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD NOV PY 2016 VL 9 IS 3 DI 10.3835/plantgenome2016.03.0035 PG 16 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA EJ3NX UT WOS:000393120000016 ER PT J AU Kool, D Heitman, JL Lazarovitch, N Agam, N Sauer, TJ Ben-Gal, A AF Kool, Dilia Heitman, Joshua L. Lazarovitch, Naftali Agam, Nurit Sauer, Thomas J. Ben-Gal, Alon TI In Situ Thermistor Calibration for Improved Measurement of Soil Temperature Gradients SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID TERM RELATIVE STABILITY; THERMAL-PROPERTIES; FIELD-EVALUATION; WATER CONTENT; HEAT-FLUX; PROBE; EVAPORATION AB Accurate measurement of soil temperature gradients is important for the estimation of soil heat flux and latent heat flux, both major components of the surface energy balance. Soil temperature gradients are commonly measured using heat-pulse sensors equipped with thermistors. In this study, individual thermistors showed absolute temperature differences on the order of 0.2 degrees C when placed under uniform temperature conditions. These differences compromised measurement of soil temperature gradients over small depth increments and/or conditions with relatively minor variation in temperatures. An in situ calibration approach was found to reduce the uncertainty between thermistors to about 0.05 degrees C in a vineyard under arid conditions. In situ calibration results were similar to laboratory results before and after field deployment for temperatures ranging between 4 and 60 degrees C. Thermistor offsets were found to change very little over a 5-yr period, indicating that pre- or post-laboratory calibration could be sufficient. The in situ approach can be useful when calibration prior to field deployment is unavailable and/or sensor failure prevents post-field calibration. C1 [Kool, Dilia; Ben-Gal, Alon] Agr Res Org, Gilat Res Ctr, Inst Soil Water & Environm Sci, IL-50250 Bet Dagan, Israel. [Kool, Dilia; Lazarovitch, Naftali; Agam, Nurit] Ben Gurion Univ Negev, Wyler Dept Dryland Agr, French Associates Inst Agr & Biotechnol Drylands, Jacob Blaustein Inst Desert Res, Sede Boqer Campus, IL-84105 Beer Sheva, Israel. [Heitman, Joshua L.] North Carolina State Univ, Dept Soil Sci, Raleigh, NC 27695 USA. [Sauer, Thomas J.] ARS, Natl Lab Agr & Environm, USDA, Ames, IA 50011 USA. RP Ben-Gal, A (reprint author), Agr Res Org, Gilat Res Ctr, Inst Soil Water & Environm Sci, IL-50250 Bet Dagan, Israel. EM bengal@volcani.agri.gov.il FU BARD [US-4262-09]; United States-Israel Binational Agricultural Research and Development Fund; I-CORE Program of the Planning and Budgeting Committee; Israel Science Foundation [152/11]; U.S. NSF [1215864] FX This research was supported by Research Grant No. US-4262-09 from BARD, the United States-Israel Binational Agricultural Research and Development Fund, and was partially supported by the I-CORE Program of the Planning and Budgeting Committee, the Israel Science Foundation (grant no. 152/11), and U.S. NSF Grant No. 1215864. NR 15 TC 0 Z9 0 U1 1 U2 1 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD NOV-DEC PY 2016 VL 80 IS 6 BP 1514 EP 1519 DI 10.2136/sssaj2016.05.0134 PG 6 WC Soil Science SC Agriculture GA EJ6ON UT WOS:000393338800009 ER PT J AU Parkin, TB Kaspar, TC Jaynes, DB Moorman, TB AF Parkin, Timothy B. Kaspar, Thomas C. Jaynes, Dan B. Moorman, Thomas B. TI Rye Cover Crop Effects on Direct and Indirect Nitrous Oxide Emissions SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID GREENHOUSE-GAS EMISSIONS; US CORN-BELT; AGRICULTURAL FIELDS; NITRATE LOSSES; N2O EMISSIONS; SOIL; DENITRIFICATION; FERTILIZER; WATER; SYSTEMS AB Winter cover crops can have a pronounced effect on N cycling in agricultural ecosystems. By reducing available soil mineral N during active growth and by providing a substrate for denitrifying bacteria after they are killed, cover crops can potentially influence soil N2O emissions. However, there have been conflicting reports regarding cover crop effects on increasing or decreasing N2O emissions. In this study, direct soil emissions of N2O were measured for a 10-yr period in a corn (Zea mays L.)-soybean [Glycine max (L.) Merr.] rotation, with and without a winter rye (Secale cereale L.) cover crop. Additionally, NO3 leaching losses during the period were measured and used to estimate indirect N2O emissions by applying the Intergovernmental Panel on Climate Change (IPCC) EF5 emission factor of 0.0075 kg N2O-N kg(-1) NO3-N leached. Direct N2O emissions measurements were measured using vented static chambers from March 2004 through December 2012. Annual N2O emissions in corn years were significantly greater than emissions in soybean years (P = 0.013). Under corn annual direct N2O emissions ranged from 2.3 to 12.5 kg N2O-N ha(-1) yr(-1), and under soybean annual emissions ranged from 1.02 to 6.94 kg N2O-N ha(-1) yr(-1). Cumulative direct N2O emissions were calculated each year by numerical integration and summed to compute 10-yr cumulative emissions for the rotation. Total direct emissions during the 10-yr period in the rye and no-rye treatments were not significantly different. The rye cover crop did effect estimated indirect emissions (P = 0.046), which were 1.25 kg N2O-N ha(-1) yr(-1) in the rye treatment and 2.69 kg N2O-N ha(-1) yr(-1) in the no-rye treatment. There was no significant difference (P = 0.155) between 10-yr cumulative total N2O emissions (direct + indirect) of the rye cover crop treatment (55.7 kg N2O-N ha(-1)) and the no cover crop treatment (63.8 kg N2O-N ha(-1)). C1 [Parkin, Timothy B.; Kaspar, Thomas C.; Jaynes, Dan B.; Moorman, Thomas B.] ARS, USDA, Natl Lab Agr & Environm, 1015 N Univ Blvd, Ames, IA 50011 USA. RP Parkin, TB (reprint author), ARS, USDA, Natl Lab Agr & Environm, 1015 N Univ Blvd, Ames, IA 50011 USA. EM tim.parkin@ars.usda.gov FU USDA-ARS GRACEnet project FX We appreciate the assistance of O. Smith, K. Heikens, B. Knutson, R. Hartwig, A. Morrow, K. Kohler, K. Headley, J. Seevers, A. Oesterreich, E. Greene, and N. Schuchmann in establishing the site, collecting data, analyzing samples, and managing the field plots. The cooperation of Iowa State University and Kent Burns is also appreciated. This research is in support of the USDA-ARS GRACEnet project. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. NR 44 TC 0 Z9 0 U1 3 U2 3 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD NOV-DEC PY 2016 VL 80 IS 6 BP 1551 EP 1559 DI 10.2136/sssaj2016.04.0120 PG 9 WC Soil Science SC Agriculture GA EJ6ON UT WOS:000393338800013 ER PT J AU Cai, ZJ Gao, SD Hendratna, A Duan, YH Xu, MG Hanson, BD AF Cai, Zejiang Gao, Suduan Hendratna, Aileen Duan, Yinghua Xu, Minggang Hanson, Bradley D. TI Key Factors, Soil Nitrogen Processes, and Nitrite Accumulation Affecting Nitrous Oxide Emissions SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID NITRIFIER DENITRIFICATION; N2O EMISSIONS; NITRIFICATION INHIBITOR; NONLINEAR RESPONSE; AMMONIA-OXIDATION; CROPPING SYSTEM; GLOBAL CHANGE; FERTILIZER; GASES; WATER AB A better understanding of the factors and processes affecting N2O emissions is essential for developing mitigation strategies. This research aimed to examine the factors and processes affecting N2O emissions and N dynamics. Laboratory incubation experiments examined the effects of N (urea) application rate (0-150 mg N kg(-1)); soil water content (5-30%, w/w); temperature (10-40 degrees C); and incorporation of biochar (1%, w/w), a urease inhibitor (Agrotain Ultra), and a nitrification inhibitor (N-Serve 24) on N2O emissions and N transformation dynamics in a Hanford sandy loam soil. Nitrous oxide emissions, soil pH, and mineral N species were monitored for 35 d. Peak emission rates and cumulative losses of N2O increased more than the N application rate increased. Soil water content at 20 and 30% [ above the water holding capacity (WHC) of 12%] resulted in much higher total N2O emissions (9.3 and 8.0% of total soil inorganic N, respectively) than that from 5 and 10% water content (0.2 and 0.3%, respectively). Increasing soil water content above WHC caused higher N2O emissions than increasing the soil temperature, which demonstrates that soil moisture is more significant in affecting the process. Nitrite concentration was highly correlated with N2O emissions within two water content ranges (above or below WHC). Amendment with biochar, Agrotain Ultra, and N-Serve 24 reduced N2O emissions by 74, 78, and 74%, respectively. This research provide further understanding of the processes affecting N2O emissions from soil, which can assist in developing management practices. C1 [Cai, Zejiang; Duan, Yinghua; Xu, Minggang] Chinese Acad Agr Sci, Natl Engn Lab Improving Qual Arable Land, Inst Agr Resources & Reg Planning, Beijing 100081, Peoples R China. [Cai, Zejiang] Qiyang Agroecosyst Natl Field Expt Stn, Changsha 426182, Hunan, Peoples R China. [Gao, Suduan; Hendratna, Aileen] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Hanson, Bradley D.] Univ Calif, Dept Plant Sci, Davis, CA 95616 USA. RP Cai, ZJ (reprint author), Chinese Acad Agr Sci, Natl Engn Lab Improving Qual Arable Land, Inst Agr Resources & Reg Planning, Beijing 100081, Peoples R China.; Cai, ZJ (reprint author), Qiyang Agroecosyst Natl Field Expt Stn, Changsha 426182, Hunan, Peoples R China. EM caizejiang@caas.cn FU National Natural Science Foundation of China [41301309] FX The majority of the research experiments were conducted by the senior author at the USDA Agricultural Research Service, San Joaquin Valley Agricultural Sciences Center, Parlier, CA, with partial support from the National Natural Science Foundation of China (41301309). We are also grateful to Mr. Bruce Mackey for his advice on the statistical analysis of our data. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA, the Chinese Academy of Agricultural Sciences (CAAS), and University of California, Davis (UC Davis). USDA, CAAS, and UC Davis are equal opportunity providers and employers. NR 52 TC 0 Z9 0 U1 3 U2 3 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD NOV-DEC PY 2016 VL 80 IS 6 BP 1560 EP 1571 DI 10.2136/sssaj2016.03.0089 PG 12 WC Soil Science SC Agriculture GA EJ6ON UT WOS:000393338800014 ER PT J AU Wang, JP Monger, C Wang, XJ Serena, M Leinauer, B AF Wang, Jiaping Monger, Curtis Wang, Xiujun Serena, Matteo Leinauer, Bernhard TI Carbon Sequestration in Response to Grassland-Shrubland-Turfgrass Conversions and a Test for Carbonate Biomineralization in Desert Soils, New Mexico, USA SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID STABLE ISOTOPIC COMPOSITION; PEDOGENIC CARBONATES; INORGANIC CARBON; CLIMATE-CHANGE; SEMIDESERT GRASSLAND; CALCIFIED FILAMENTS; CALCIUM-CARBONATE; OXYGEN ISOTOPES; ORGANIC-MATTER; RECORD AB This study uses an experimental pedology approach to examine (i) how the conversion of native C-4 grassland to C-3 woody shrubs then to irrigated C-4 turfgrass affects both soil organic C (SOC) and soil inorganic C (SIC) and (ii) whether SIC can be enhanced by microbial biomineralization. Three sites were studied in the Chihuahuan Desert of New Mexico. At each site, SOC, SIC, and their delta C-13 values were measured on control soil samples and compared to samples treated with liquid growth medium and Ca(OH)(2) at 10 and 40 cm. The treated samples were left to react for 1 mo in February, May, and August of 2014. Using a space-for-time substitution, soil organic C decreased when native grasslands converted to desert shrubs, then sharply increased after desert shrubs were converted to irrigated turfgrass. Most surprising, however, was the increase of SIC in the turfgrass site, which doubled in 6 yr. The delta C-13 values of both SOC and SIC reflected the change from C-4 to C-3 then back to C-4 vegetation and showed how rapidly SOC and SIC can change their isotopic signatures. Soil inorganic C formation was slightly higher for the liquid growth medium, but no statistically significant differences were observed between the treatments and control samples. In addition, no biomineralization was observed with microscopy, perhaps because the 1-mo reaction time was too short and the amount applied was too small. Although SIC is typically viewed as a soil mineral that requires centuries to accumulate, our study indicate that SIC can be generated in months to years if the soil environment is suitable. C1 [Wang, Jiaping] Shihezi Univ, Coll Agron, Shihezi 832003, Peoples R China. [Wang, Jiaping; Wang, Xiujun] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi 830011, Peoples R China. [Monger, Curtis; Serena, Matteo; Leinauer, Bernhard] New Mexico State Univ, Las Cruces, NM 88001 USA. [Monger, Curtis] USDA, Natl Soil Survey Ctr, Lincoln, NE 68508 USA. [Wang, Xiujun] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20740 USA. RP Monger, C (reprint author), New Mexico State Univ, Las Cruces, NM 88001 USA.; Monger, C (reprint author), USDA, Natl Soil Survey Ctr, Lincoln, NE 68508 USA. EM curtis.monger@lin.usda.gov FU National Natural Science Foundation of China [41403072]; Chinese Scholarship Council; National Science Foundation [DEB 125828]; New Mexico State University Agricultural Experiment Station FX The authors are grateful for the financial support from the National Natural Science Foundation of China (grant No. 41403072) and the Chinese Scholarship Council. Funding was also provided by the National Science Foundation to the Jornada Basin Long Term Ecological Research Program (DEB 125828) and the New Mexico State University Agricultural Experiment Station. NR 67 TC 0 Z9 0 U1 2 U2 2 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD NOV-DEC PY 2016 VL 80 IS 6 BP 1591 EP 1603 DI 10.2136/sssaj2016.03.0061 PG 13 WC Soil Science SC Agriculture GA EJ6ON UT WOS:000393338800017 ER PT J AU Balkcom, KS Duzy, LM Mitchell, CC Delaney, DP AF Balkcom, Kipling S. Duzy, Leah M. Mitchell, Charles C. Delaney, Dennis P. TI A Simple Approach to Enhance Multiprobe Soil Cone Penetrometer Analyses SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID UNDER-THE-CURVE; TILLAGE SYSTEMS; COTTON; COMPACTION; STRENGTH; ROTATION AB Soil penetrometers measure penetration resistance to characterize soil strength for different management practices, primarily tillage. Our objective was to demonstrate a simple approach to quantify soil strength differences among treatments collected with a multiprobe cone penetrometer using cone index measurements to complement contour graphs and statistical analyses. The approach involves obtaining average cone index values by row position and calculating the area under the curve for cone index (AUC(C.I.)), with units of megapascal-centimeters (MPa-cm). Data were collected from a tillage and cover crop experiment in Prattville, AL (November 2009), and at the Old Rotation experiment in Auburn, AL (December 2014). Cone index values were averaged across depths for five row positions perpendicular to the center of the row (0 cm) and 22.5 and 45 cm on either side of the crop row. The approach identified that three conservation tillage implements reduced penetration resistance compared with no tillage based on AUC(C.I.) values from the Prattville experiment. Although no statistical comparisons were possible from the unreplicated Old Rotation experiment, AUC(C.I.) values were numerically lower for the 3-yr rotation compared with continuous cotton (Gossypium hirsutum L.) and the 2-yr rotation. Results from both sets of cone index measurements demonstrated that the area under the curve (AUC) approach can be used to quantitatively distinguish among treatments. Results from the AUC approach can be used to facilitate simple comparisons among treatments in field experiments and aid in the interpretation of traditional statistical analyses combined with contour graphs. C1 [Balkcom, Kipling S.; Duzy, Leah M.] ARS, USDA, Natl Soil Dynam Lab, 411 S Donahue Dr, Auburn, AL 36832 USA. [Mitchell, Charles C.; Delaney, Dennis P.] Auburn Univ, Crop Soil & Environm Sci Dept, 201 Funchess Hall, Auburn, AL 36849 USA. RP Balkcom, KS (reprint author), ARS, USDA, Natl Soil Dynam Lab, 411 S Donahue Dr, Auburn, AL 36832 USA. EM kip.balkcom@ars.usda.gov NR 25 TC 0 Z9 0 U1 0 U2 0 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD NOV-DEC PY 2016 VL 80 IS 6 BP 1619 EP 1628 DI 10.2136/sssaj2016.05.0157 PG 10 WC Soil Science SC Agriculture GA EJ6ON UT WOS:000393338800019 ER PT J AU Hammac, WA Stott, DE Karlen, DL Cambardella, CA AF Hammac, W. Ashley Stott, Diane E. Karlen, Douglas L. Cambardella, Cynthia A. TI Crop, Tillage, and Landscape Effects on Near-Surface Soil Quality Indices in Indiana SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID MANAGEMENT ASSESSMENT FRAMEWORK; CHLOROFORM FUMIGATION; AGGREGATE STABILITY; GREAT-PLAINS; GRAIN-YIELD; NITROGEN; CARBON; CORN; SYSTEM; IOWA AB Soil quality is a critical link between land management and water quality. We aimed to assess soil quality within the Cedar Creek Watershed, a pothole- dominated subwatershed within the St. Joseph River watershed that drains into the Western Lake Erie Basin in northeastern Indiana. The Soil Management Assessment Framework (SMAF) with 10 soil quality indicators was used to assess inherent and dynamic soil and environmental characteristics across crop rotations, tillage practices, and landscape positions. Surface physical, chemical, and nutrient component indices were high, averaging 90, 93, and 98% of the optimum, respectively. Surface biology had the lowest component score, averaging 69% of the optimum. Crop rotation, tillage, and landscape position effects were assessed using ANOVA. Crop selection had a greater impact on soil quality than tillage, with perennial grass systems having higher values than corn (Zea mays L.) or soybean [Glycine max (L.) Merr.]. Furthermore, soybean rotations often scored higher than corn rotations. Uncultivated perennial grass systems had higher overall soil quality index (SQI) values and physical, chemical, and biological component values than no-till or chisel-disk systems. Chisel-disk effects on overall and component SQI values were generally not significantly different from no-till management except for a few physical indicators. Toe-slopes had higher physical, biological, and overall SQI values than summit positions but toe-slope values were not significantly different from those of mid-slope positions. This work highlights the positive effects of perennial grass systems, the negative effects of corn-based systems, and the neutral effects of tillage on soil quality. C1 [Hammac, W. Ashley] USDA ARS, Natl Soil Eros Res Lab, 275 S Russell St, W Lafayette, IN 47907 USA. [Stott, Diane E.] USDA NRCS, Soil Hlth Div, 915 W State St, W Lafayette, IN 47907 USA. [Karlen, Douglas L.; Cambardella, Cynthia A.] USDA ARS, Natl Lab Agr & Environm, 2110 Univ Blvd, Ames, IA 50011 USA. RP Hammac, WA (reprint author), USDA ARS, Natl Soil Eros Res Lab, 275 S Russell St, W Lafayette, IN 47907 USA. EM Ashley.Hammac@ars.usda.gov FU USDA-ARS under the ARS Conservation Effects Assessment Project FX We thank Rhonda Graef, Larry Pelleck, and Jody Omacht for their excellent technical assistance; Stan Livingston for his aid in selecting sites and collecting samples; and Deb Palmquist for her aid with statistical analysis. This publication is based on work supported by the USDA-ARS under the ARS Conservation Effects Assessment Project. The USDA is an equal opportunity provider and employer. NR 69 TC 0 Z9 0 U1 1 U2 1 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD NOV-DEC PY 2016 VL 80 IS 6 BP 1638 EP 1652 DI 10.2136/sssaj2016.09.0282 PG 15 WC Soil Science SC Agriculture GA EJ6ON UT WOS:000393338800021 ER PT J AU Van Beusekom, AE Viger, RJ AF Van Beusekom, A. E. Viger, R. J. TI A glacier runoff extension to the Precipitation Runoff Modeling System SO JOURNAL OF GEOPHYSICAL RESEARCH-EARTH SURFACE LA English DT Article DE glacier runoff; Precipitation Runoff Modeling System; Alaska ID MASS-BALANCE; CLIMATE-CHANGE; GLOBAL OPTIMIZATION; HYDROLOGICAL MODEL; ALPINE GLACIERS; 20-1ST CENTURY; ICE-THICKNESS; SENSITIVITY; VOLUME; PARAMETERIZATION AB A module to simulate glacier runoff, PRMSglacier, was added to PRMS (Precipitation Runoff Modeling System), a distributed-parameter, physical-process hydrological simulation code. The extension does not require extensive on-glacier measurements or computational expense but still relies on physical principles over empirical relations as much as is feasible while maintaining model usability. PRMSglacier is validated on two basins in Alaska, Wolverine, and Gulkana Glacier basin, which have been studied since 1966 and have a substantial amount of data with which to test model performance over a long period of time covering a wide range of climatic and hydrologic conditions. When error in field measurements is considered, the Nash-Sutcliffe efficiencies of streamflow are 0.87 and 0.86, the absolute bias fractions of the winter mass balance simulations are 0.10 and 0.08, and the absolute bias fractions of the summer mass balances are 0.01 and 0.03, all computed over 42years for the Wolverine and Gulkana Glacier basins, respectively. Without taking into account measurement error, the values are still within the range achieved by the more computationally expensive codes tested over shorter time periods. C1 [Van Beusekom, A. E.] US Forest Serv, USDA, Int Inst Trop Forestry, Rio Piedras, PR 00926 USA. [Viger, R. J.] US Geol Survey, Box 25046, Denver, CO 80225 USA. RP Van Beusekom, AE (reprint author), US Forest Serv, USDA, Int Inst Trop Forestry, Rio Piedras, PR 00926 USA. EM beusekom@usgs.gov OI Van Beusekom, Ashley/0000-0002-6996-978X FU Alaska Climate Science Center; Northwest Climate Science Center FX Output data are available on Science Base https://dx.doi.org/10.5066/F75T3HMV (sources for input data are cited in the manuscript). This work was supported by the Alaska and Northwest Climate Science Centers. We would like to thank our anonymous reviewers and our colleagues at the U.S. Geological Survey, including Timothy Brabets and Lauren Hay. Any use of trade, product, or firms names is for descriptive purposes only and does not imply endorsement by the U.S. government. NR 85 TC 1 Z9 1 U1 1 U2 1 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-9003 EI 2169-9011 J9 J GEOPHYS RES-EARTH JI J. Geophys. Res.-Earth Surf. PD NOV PY 2016 VL 121 IS 11 BP 2001 EP 2021 DI 10.1002/2015JF003789 PG 21 WC Geosciences, Multidisciplinary SC Geology GA EI9KD UT WOS:000392825900003 ER PT J AU Anderson, TK Macken, CA Lewis, NS Scheuermann, RH Van Reeth, K Brown, IH Swenson, SL Simon, G Saito, T Berhane, Y Ciacci-Zanella, J Pereda, A Davis, CT Donis, RO Webby, RJ Vincent, AL AF Anderson, Tavis K. Macken, Catherine A. Lewis, Nicola S. Scheuermann, Richard H. Van Reeth, Kristien Brown, Ian H. Swenson, Sabrina L. Simon, Gaelle Saito, Takehiko Berhane, Yohannes Ciacci-Zanella, Janice Pereda, Ariel Davis, C. Todd Donis, Ruben O. Webby, Richard J. Vincent, Amy L. TI A Phylogeny-Based Global Nomenclature System and Automated Annotation Tool for H1 Hemagglutinin Genes from Swine Influenza A Viruses SO MSPHERE LA English DT Article DE H1N1; H1N2; influenza A virus; molecular epidemiology; nomenclature; swine; virus evolution ID UNITED-STATES; EUROPEAN SWINE; NORTH-AMERICA; PIGS; EVOLUTION; TRANSMISSION; REASSORTMENT; VACCINATION; ORIGIN; H3N2 AB The H1 subtype of influenza A viruses (IAVs) has been circulating in swine since the 1918 human influenza pandemic. Over time, and aided by further introductions from nonswine hosts, swine H1 viruses have diversified into three genetic lineages. Due to limited global data, these H1 lineages were named based on colloquial context, leading to a proliferation of inconsistent regional naming conventions. In this study, we propose rigorous phylogenetic criteria to establish a globally consistent nomenclature of swine H1 virus hemagglutinin (HA) evolution. These criteria applied to a data set of 7,070 H1 HA sequences led to 28 distinct clades as the basis for the nomenclature. We developed and implemented a web-accessible annotation tool that can assign these biologically informative categories to new sequence data. The annotation tool assigned the combined data set of 7,070 H1 sequences to the correct clade more than 99% of the time. Our analyses indicated that 87% of the swine H1 viruses from 2010 to the present had HAs that belonged to 7 contemporary cocirculating clades. Our nomenclature and web-accessible classification tool provide an accurate method for researchers, diagnosticians, and health officials to assign clade designations to HA sequences. The tool can be updated readily to track evolving nomenclature as new clades emerge, ensuring continued relevance. A common global nomenclature facilitates comparisons of IAVs infecting humans and pigs, within and between regions, and can provide insight into the diversity of swine H1 influenza virus and its impact on vaccine strain selection, diagnostic reagents, and test performance, thereby simplifying communication of such data. IMPORTANCE A fundamental goal in the biological sciences is the definition of groups of organisms based on evolutionary history and the naming of those groups. For influenza A viruses (IAVs) in swine, understanding the hemagglutinin (HA) genetic lineage of a circulating strain aids in vaccine antigen selection and allows for inferences about vaccine efficacy. Previous reporting of H1 virus HA in swine relied on colloquial names, frequently with incriminating and stigmatizing geographic toponyms, making comparisons between studies challenging. To overcome this, we developed an adaptable nomenclature using measurable criteria for historical and contemporary evolutionary patterns of H1 global swine IAVs. We also developed a web-accessible tool that classifies viruses according to this nomenclature. This classification system will aid agricultural production and pandemic preparedness through the identification of important changes in swine IAVs and provides terminology enabling discussion of swine IAVs in a common context among animal and human health initiatives. C1 [Anderson, Tavis K.; Vincent, Amy L.] USDA ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, Ames, IA 50011 USA. [Macken, Catherine A.] Univ Auckland, Bioinformat Inst, Auckland, New Zealand. [Lewis, Nicola S.] Univ Cambridge, Dept Zool, Cambridge, England. [Scheuermann, Richard H.] J Craig Venter Inst, La Jolla, CA USA. [Scheuermann, Richard H.] Univ Calif San Diego, Dept Pathol, San Diego, CA 92103 USA. [Van Reeth, Kristien] Univ Ghent, Fac Vet Med, Virol Lab, Merelbeke, Belgium. [Brown, Ian H.] Anim & Plant Hlth Agcy, Weybridge, Surrey, England. [Swenson, Sabrina L.] USDA APHIS, Natl Vet Serv Labs, Ames, IA USA. [Simon, Gaelle] ANSES, Ploufragan Plouzane Lab, Swine Virol Immunol Unit, Ploufragan, France. [Saito, Takehiko] Natl Agr & Food Res Org, Natl Inst Anim Hlth, Div Transboundary Anim Dis, Ibaraki, Japan. [Berhane, Yohannes] Canadian Food Inspect Agcy, Natl Ctr Foreign Anim Dis, Winnipeg, MB, Canada. [Ciacci-Zanella, Janice] Embrapa Swine & Poultry, Anim Hlth & Genet Lab, Concordia, SC USA. [Pereda, Ariel] CICVyA INTA, Inst Patobiol, Buenos Aires, DF, Argentina. [Davis, C. Todd; Donis, Ruben O.] Ctr Dis Control & Prevent, Influenza Div, Natl Ctr Immunizat & Resp Dis, Atlanta, GA USA. [Webby, Richard J.] St Jude Childrens Res Hosp, Dept Infect Dis, 332 N Lauderdale St, Memphis, TN 38105 USA. EM amy.vincent@ars.usda.gov OI Anderson, Tavis/0000-0002-3138-5535; Scheuermann, Richard/0000-0003-1355-892X FU HHS \ NIH \ National Institute of Allergy and Infectious Diseases (NIAID) [HHSN272201400008C, HHSN266200700005C, HHSN272201400006C, HHSN266200400041C]; European Commission (EC) [259949]; USDA \ Agricultural Research Service (ARS) [58-3625-4-070, 58-3625-2103F]; DOE \ LDRD \ Oak Ridge Institute for Science and Education (ORISE) [DE-AC05-06OR23100]; USDA \ Animal and Plant Health Inspection Service (APHIS) FX This work, including the efforts of Nicola S. Lewis and Amy L. Vincent, was funded by HHS vertical bar NIH vertical bar National Institute of Allergy and Infectious Diseases (NIAID) (HHSN272201400008C). This work, including the efforts of Richard J. Webby, was funded by HHS vertical bar NIH vertical bar National Institute of Allergy and Infectious Diseases (NIAID) (HHSN266200700005C and HHSN272201400006C). This work, including the efforts of Catherine A. Macken and Richard H. Scheuermann, was funded by HHS vertical bar NIH vertical bar National Institute of Allergy and Infectious Diseases (NIAID) (HHSN266200400041C and HHSN272201400006C). This work, including the efforts of Nicola S. Lewis, Kristien Van Reeth, Ian H. Brown, and Gaelle Simon, was funded by European Commission (EC) (259949). This work, including the efforts of Tavis K. Anderson, was funded by USDA vertical bar Agricultural Research Service (ARS) (58-3625-4-070). This work, including the efforts of Nicola S. Lewis, was funded by USDA vertical bar Agricultural Research Service (ARS) (58-3625-2103F). This work, including the efforts of Tavis K. Anderson, was funded by DOE vertical bar LDRD vertical bar Oak Ridge Institute for Science and Education (ORISE) (DE-AC05-06OR23100). This work, including the efforts of Sabrina L. Swenson, was funded by USDA vertical bar Animal and Plant Health Inspection Service (APHIS). This work, including the efforts of Amy L. Vincent, was funded by USDA vertical bar Agricultural Research Service (ARS). NR 61 TC 0 Z9 0 U1 4 U2 4 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 2379-5042 J9 MSPHERE JI mSphere PD NOV-DEC PY 2016 VL 1 IS 6 AR e00275-16 DI 10.1128/mSphere.00275-16 PG 14 WC Microbiology SC Microbiology GA EI6EM UT WOS:000392587800010 ER PT J AU Rodhouse, TJ Philippi, TE Monahan, WB Castle, KT AF Rodhouse, Thomas J. Philippi, Thomas E. Monahan, William B. Castle, Kevin T. TI A macroecological perspective on strategic bat conservation in the US National Park Service SO ECOSPHERE LA English DT Article DE Chiroptera; climate change; conservation; land use change; national parks; prioritization; protected areas; Special Feature: Science for Our National Parks' Second Century; species-area relationship; species-energy relationship; white-nose syndrome; wind energy ID WHITE-NOSE SYNDROME; DYNAMIC DISTRIBUTION MODELS; SPECIES RICHNESS; CLIMATE-CHANGE; UNITED-STATES; LATITUDINAL GRADIENTS; EXTINCTION RISK; NORTH-AMERICA; NEW-WORLD; LAND-USE AB North American bat populations face unprecedented threats from disease and rapid environmental change, requiring a commensurate strategic conservation response. Protected-area networks have tremendous potential to support coordinated resource protection, disease surveillance, and population monitoring that could become a cornerstone of 21st-century bat conservation. To motivate this idea, we develop a macroecological perspective about bat diversity and associated conservation challenges and opportunities on U.S. National Park Service (NPS) lands. We compared occurrence records from parks against published range maps. Only 55 (19%) of parks reported as present >= 90% of the bat species expected based on range maps, highlighting the information-gap challenge. Discrepancies suggest substantial under-reporting and under-sampling of bats on NPS lands; inadequate range maps and habitat specificity are implicated for some species. Despite these discrepancies, 50 species, including several range-restricted and endangered taxa, were reported in at least one park unit, including those in the Caribbean and tropical Pacific. Species richness increased with park area at a rate (z) of similar to 0.1, a pattern confounded by covariation with latitude, elevation, and habitat. When accounting for these factors, richness decreased predictably at higher latitudes and increased at mid-elevations and with greater numbers of keystone underground habitat structures (caves and mines), reflecting a strong species-energy relationship. The inclusion of covariates that represented percentage of natural vs. human-modified (converted) landscapes and elevation range-a proxy for environmental heterogeneity-was uninformative. White-nose syndrome (WNS) presents a tremendous challenge to the NPS: All 12 species currently known to be affected by the disease or to host the causal fungus are represented in the NPS system. One hundred and twenty-seven NPS parks are in counties currently or likely to become WNS-positive by 2026. All parks are expected to experience increasing temperatures in coming decades; forecasted climate change velocity is particularly high (>1 SD) for 50 parks. Seventeen parks are in the vicinity of high (>1 SD) wind turbine density. Based on these biogeographic patterns, we suggest ways to prioritize NPS parks for additional inventories, monitoring, and resource protection. Our results demonstrate how macroecology and bioinformatics together can guide strategic conservation capacity-building among protected areas. C1 [Rodhouse, Thomas J.] Natl Pk Serv, Upper Columbia Basin Network, 650 SW Columbia St, Bend, OR 97702 USA. [Philippi, Thomas E.] Natl Pk Serv, Inventory & Monitoring Div, 1800 Cabrillo Mem Dr, San Diego, CA 92106 USA. [Monahan, William B.] USDA Forest Serv, Forest Hlth Technol Enterprise Team, 2150A Ctr Ave,Suite 331, Ft Collins, CO 80526 USA. [Castle, Kevin T.] Natl Pk Serv, Biol Resources Div, 1201 Oak Ridge Dr, Ft Collins, CO 80525 USA. [Castle, Kevin T.] Wildlife Vet Consulting LLC, 840 Sundance Dr, Livermore, CO 80536 USA. RP Rodhouse, TJ (reprint author), Natl Pk Serv, Upper Columbia Basin Network, 650 SW Columbia St, Bend, OR 97702 USA. EM Tom_Rodhouse@nps.gov FU NPS Biological Resources Division; Inventory and Monitoring Division FX We thank A. Loar for assistance with NPSpecies. D. Pate provided park counts of cave and karst features. J. Burghardt provided park counts of abandoned mine features. S. Maher provided GIS data on projected spread of WNS across the continental United States. P. Cryan and M. Verant provided helpful comments during preparation of the manuscript. We thank the two anonymous reviewers for helpful feedback and suggestions. Funding for this project was provided by the NPS Biological Resources Division and Inventory and Monitoring Division. NR 89 TC 0 Z9 0 U1 7 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD NOV PY 2016 VL 7 IS 11 AR e01576 DI 10.1002/ecs2.1576 PG 21 WC Ecology SC Environmental Sciences & Ecology GA EB2UI UT WOS:000387217700025 ER PT J AU Costello, RB Elin, RJ Rosanoff, A Wallace, TC Guerrero-Romero, F Hruby, A Lutsey, PL Nielsen, FH Rodriguez-Moran, M Song, YQ Van Horn, LV AF Costello, Rebecca B. Elin, Ronald J. Rosanoff, Andrea Wallace, Taylor C. Guerrero-Romero, Fernando Hruby, Adela Lutsey, Pamela L. Nielsen, Forrest H. Rodriguez-Moran, Martha Song, Yiqing Van Horn, Linda V. TI Perspective: The Case for an Evidence-Based Reference Interval for Serum Magnesium: The Time Has Come SO ADVANCES IN NUTRITION LA English DT Article DE serum magnesium; plasma magnesium; magnesium deficiency; reference interval; chronic disease ID BONE-MINERAL DENSITY; IMPROVES INSULIN SENSITIVITY; ACUTE MYOCARDIAL-INFARCTION; CONTROLLED RANDOMIZED-TRIAL; PLACEBO-CONTROLLED TRIAL; TYPE-2 DIABETIC-PATIENTS; ISCHEMIC-HEART-DISEASE; NUTRITION SURVEY 2006; HIGH BLOOD-PRESSURE; ALL-CAUSE MORTALITY AB The 2015 Dietary Guidelines Advisory Committee indicated that magnesium was a shortfall nutrient that was underconsumed relative to the Estimated Average Requirement (EAR) for many Americans. Approximately 50% of Americans consume less than the EAR for magnesium, and some age groups consume substantially less. A growing body of literature from animal, epidemiologic, and clinical studies has demonstrated a varied pathologic role for magnesium deficiency that includes electrolyte, neurologic, musculoskeletal, and inflammatory disorders; osteoporosis; hypertension; cardiovascular diseases; metabolic syndrome; and diabetes. Studies have also demonstrated that magnesium deficiency is associated with several chronic diseases and that a reduced risk of these diseases is observed with higher magnesium intake or supplementation. Subclinical magnesium deficiency can exist despite the presentation of a normal status as defined within the current serum magnesium reference interval of 0.75-0.95 mmol/L. This reference interval was derived from data from NHANES I (1974), which was based on the distribution of serum magnesium in a normal population rather than clinical outcomes. What is needed is an evidenced-based serum magnesium reference interval that reflects optimal health and the current food environment and population. We present herein data from an array of scientific studies to support the perspective that subclinical deficiencies in magnesium exist, that they contribute to several chronic diseases, and that adopting a revised serum magnesium reference interval would improve clinical care and public health. C1 [Costello, Rebecca B.; Rosanoff, Andrea] Ctr Magnesium Educ & Res, Pahoa, HI 96778 USA. [Elin, Ronald J.] Univ Louisville, Dept Pathol & Lab Med, Louisville, KY USA. [Wallace, Taylor C.] George Mason Univ, Dept Nutr & Food Studies, Fairfax, VA USA. [Guerrero-Romero, Fernando; Rodriguez-Moran, Martha] Mexican Social Secur Inst, Biomed Res Unit, Durango, Mexico. [Hruby, Adela] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr, Nutr Epidemiol Program, Boston, MA USA. [Lutsey, Pamela L.] Univ Minnesota, Sch Publ Hlth, Div Epidemiol & Community Hlth, Minneapolis, MN USA. [Nielsen, Forrest H.] Grand Forks Human Nutr Res Ctr, Grand Forks, ND USA. [Song, Yiqing] Indiana Univ, Richard M Fairbanks Sch Publ Hlth, Indianapolis, IN USA. [Van Horn, Linda V.] Northwetsren Univ, Dept Prevent Med, Div Nutr, Chicago, IL USA. RP Costello, RB (reprint author), Ctr Magnesium Educ & Res, Pahoa, HI 96778 USA. EM rbcostello@earthlink.net OI Wallace, Taylor/0000-0002-9403-2745; Costello, Rebecca/0000-0003-0246-0273 FU Albion Minerals; Consumer Healthcare Products Association; Council for Responsible Nutrition; National Osteoporosis Foundation; Pharmavite; Siemens FX The perspectives gathered in this article stem in part from the workshop "Addressing an Evidence-Based Reference Interval for Total Serum Magnesium Concentration" held in April 2015 at Lowell, Massachusetts. The workshop was organized by the Center for Magnesium Education and Research and the American Society for Nutrition and was supported by contributions received from Albion Minerals, Consumer Healthcare Products Association, the Council for Responsible Nutrition, National Osteoporosis Foundation, Pharmavite, Siemens, and Dr. Forrest Nielsen (personal donation). NR 185 TC 1 Z9 1 U1 1 U2 1 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 2161-8313 EI 2156-5376 J9 ADV NUTR JI Adv. Nutr. PD NOV PY 2016 VL 7 IS 6 BP 977 EP 993 DI 10.3945/an.116.012765 PG 17 WC Nutrition & Dietetics SC Nutrition & Dietetics GA EI1ZE UT WOS:000392284700001 PM 28140318 ER PT J AU Redan, BW Buhman, KK Novotny, JA Ferruzzi, MG AF Redan, Benjamin W. Buhman, Kimberly K. Novotny, Janet A. Ferruzzi, Mario G. TI Altered Transport and Metabolism of Phenolic Compounds in Obesity and Diabetes: Implications for Functional Food Development and Assessment SO ADVANCES IN NUTRITION LA English DT Review DE phenolic compounds; flavonoids; phase II metabolism; diabetes; obesity; inflammation ID BLOOD-BRAIN-BARRIER; ELLAGIC ACID METABOLISM; GUT MICROBIAL ECOLOGY; FATTY LIVER-DISEASE; P-GLYCOPROTEIN; UDP-GLUCURONOSYLTRANSFERASE; DRUG-METABOLISM; SULFOTRANSFERASE EXPRESSION; INTESTINAL-ABSORPTION; BIOLOGICAL-ACTIVITIES AB Interest in the application of phenolic compounds from the diet or supplements for the prevention of chronic diseases has grown substantially, but the efficacy of such approaches in humans is largely dependent on the bioavailability and metabolism of these compounds. Although food and dietary factors have been the focus of intense investigation, the impact of disease states such as obesity or diabetes on their absorption, metabolism, and eventual efficacy is important to consider. These factors must be understood in order to develop effective strategies that leverage bioactive phenolic compounds for the prevention of chronic disease. The goal of this review is to discuss the inducible metabolic systems that may be influenced by disease states and how these effects impact the bioavailability and metabolism of dietary phenolic compounds. Because current studies generally report that obesity and/or diabetes alter the absorption and excretion of these compounds, this review includes a description of the absorption, conjugation, and excretion pathways for phenolic compounds and how they are potentially altered in disease states. A possible mechanism that will be discussed related to the modulation of phenolic bioavailability and metabolism may be linked to increased inflammatory status from increased amounts of adipose tissue or elevated plasma glucose concentrations. Although more studies are needed, the translation of benefits derived from dietary phenolic compounds to individuals with obesity or diabetes may require the consideration of dosing strategies or be accompanied by adjunct therapies to improve the bioavailability of these compounds. C1 [Redan, Benjamin W.; Buhman, Kimberly K.; Ferruzzi, Mario G.] Purdue Univ, Dept Nutr Sci, Interdept Nutr Program, W Lafayette, IN 47907 USA. [Ferruzzi, Mario G.] Purdue Univ, Dept Food Sci, W Lafayette, IN 47907 USA. [Novotny, Janet A.] USDA, Agr Res Serv Food Components & Hlth Lab, Beltsville, MD USA. RP Ferruzzi, MG (reprint author), North Carolina State Univ, Plants Human Hlth Inst, 600 Laureate Way, Kannapolis, NC 28081 USA. EM mario_ferruzzi@ncsu.edu FU National Science Foundation Graduate Research Fellowship Program [DGE-1333468] FX BWR is supported by the National Science Foundation Graduate Research Fellowship Program under grant DGE-1333468. NR 111 TC 1 Z9 1 U1 1 U2 1 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 2161-8313 EI 2156-5376 J9 ADV NUTR JI Adv. Nutr. PD NOV PY 2016 VL 7 IS 6 BP 1090 EP 1104 DI 10.3945/an.116.013029 PG 15 WC Nutrition & Dietetics SC Nutrition & Dietetics GA EI1ZE UT WOS:000392284700009 PM 28140326 ER PT J AU Kieffer, DA Martin, RJ Adams, SH AF Kieffer, Dorothy A. Martin, Roy J. Adams, Sean H. TI Impact of Dietary Fibers on Nutrient Management and Detoxification Organs: Gut, Liver, and Kidneys SO ADVANCES IN NUTRITION LA English DT Review DE xenobiotic; microbiota; fiber; chronic kidney disease; nonalcoholic fatty liver disease ID CHAIN FATTY-ACIDS; CHRONIC-RENAL-FAILURE; HYDROXYPROPYL METHYLCELLULOSE HPMC; INCREASED INTESTINAL PERMEABILITY; ALL-CAUSE MORTALITY; P-CRESYL SULFATE; LOW-PROTEIN DIET; GERM-FREE RATS; RESISTANT STARCH; INSULIN-RESISTANCE AB Increased dietary fiber (DF) intake elicits a wide range of physiologic effects, not just locally in the gut, but systemically. DFs can greatly alter the gut milieu by affecting the gut microbiome, which in turn influences the gut barrier, gastrointestinal immune and endocrine responses, and nitrogen cycling and microbial metabolism. These gut-associated changes can then alter the physiology and biochemistry of the body's other main nutrient management and detoxification organs, the liver and kidneys. The molecular mechanisms by which DF alters the physiology of the gut, liver, and kidneys is likely through gut-localized events (i.e., bacterial nitrogen metabolism, microbe-microbe, and microbe-host cell interactions) coupled with specific factors that emanate from the gut in response to DF, which signal to or affect the physiology of the liver and kidneys. The latter may include microbe-derived xenometabolites, peptides, or bioactive food components made available by gut microbes, inflammation signals, and gut hormones. The intent of this review is to summarize how DF alters the gut milieu to specifically affect intestinal, liver, and kidney functions and to discuss the potential local and systemic signaling networks that are involved. C1 [Kieffer, Dorothy A.; Martin, Roy J.; Adams, Sean H.] Univ Calif Davis, Grad Grp Nutr Biol, Davis, CA 95616 USA. [Kieffer, Dorothy A.; Martin, Roy J.; Adams, Sean H.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Kieffer, Dorothy A.; Martin, Roy J.] USDA ARS, Obes & Metab Res Unit, Western Human Nutr Res Ctr, Davis, CA USA. [Adams, Sean H.] Arkansas Childrens Nutr Ctr, Little Rock, AR USA. [Adams, Sean H.] Univ Arkansas Med Sci, Dept Pediat, Little Rock, AR 72205 USA. RP Adams, SH (reprint author), Univ Calif Davis, Grad Grp Nutr Biol, Davis, CA 95616 USA.; Adams, SH (reprint author), Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA.; Adams, SH (reprint author), Arkansas Childrens Nutr Ctr, Little Rock, AR USA.; Adams, SH (reprint author), Univ Arkansas Med Sci, Dept Pediat, Little Rock, AR 72205 USA. EM shadams@uams.edu FU Danish Council for Strategic Research Project [10-093526]; National Center for Advancing Translational Sciences, NIH [UL1 TR000002, TL1 TR000133]; USDA [5306-51530-019-00, 6026-51000-010-05S] FX Fiber and microbiome research by the authors was supported in part by the Danish Council for Strategic Research Project 10-093526; a T32 training award (to DAK) funded by the National Center for Advancing Translational Sciences, NIH, through grant UL1 TR000002 and linked award TL1 TR000133; and USDA projects 5306-51530-019-00 and 6026-51000-010-05S. The USDA is an equal opportunity provider and employer. NR 157 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 2161-8313 EI 2156-5376 J9 ADV NUTR JI Adv. Nutr. PD NOV PY 2016 VL 7 IS 6 BP 1111 EP 1121 DI 10.3945/an.116.013219 PG 11 WC Nutrition & Dietetics SC Nutrition & Dietetics GA EI1ZE UT WOS:000392284700011 PM 28140328 ER PT J AU Cronin, MA Leesburg, VLR AF Cronin, M. A. Leesburg, V. L. R. TI Genetic variation and differentiation in parent-descendant cattle and bison populations SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE cattle; bison; genetic variation; heterozygosity ID DISTANCE; BREEDS; LINE AB Genetic variation and differentiation at 32 microsatellite loci was quantified for parent-descendant cattle populations and parent-descendant bison (Bison bison) populations. We compared heterozygosity (Ho) and allelic richness (AR) for 587 cattle of four breeds and three lines derived from them, and 188 bison in three pairs of parent-descendant populations. Ho and AR were less in the Line 1 Hereford inbred cattle population than in the parent Hereford breed. Ho and AR were intermediate in a composite population (CGC, derived from crossing Red Angus, Charolais, and Tarentaise) compared to the three parent breeds. Crossbreeding of Line 1 with CGC resulted in an F1 generation with increased Ho and AR relative to Line 1 and CGC, followed by decreased Ho and AR in 2 backcross generations to Line1. Three transplanted wild bison populations had smaller Ho and AR than their respective parent populations. These data demonstrate that genetic variation reduced from founder effects or inbreeding can be restored with crossbreeding and gene flow. C1 [Cronin, M. A.] Univ Alaska, Sch Nat Resources & Agr Sci, Palmer Res Ctr, 1509 South Georgeson Rd, Palmer, AK 99645 USA. [Leesburg, V. L. R.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. [Cronin, M. A.] Northwest Biotechnol Co, 8415 Jupiter Dr, Anchorage, AK 99507 USA. RP Cronin, MA (reprint author), Univ Alaska, Sch Nat Resources & Agr Sci, Palmer Res Ctr, 1509 South Georgeson Rd, Palmer, AK 99645 USA.; Cronin, MA (reprint author), Northwest Biotechnol Co, 8415 Jupiter Dr, Anchorage, AK 99507 USA. EM croninm@aol.com NR 18 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD NOV PY 2016 VL 94 IS 11 BP 4491 EP 4497 DI 10.2527/jas2016-0476 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA EI6YO UT WOS:000392643800002 PM 27898962 ER PT J AU Gentry, WW Weiss, CP Meredith, CM McCollum, FT Cole, NA Jennings, JS AF Gentry, W. W. Weiss, C. P. Meredith, C. M. McCollum, F. T. Cole, N. A. Jennings, J. S. TI Effects of roughage inclusion and particle size on performance and rumination behavior of finishing beef steers SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE beef cattle; corn stalks; finishing diet; rumination ID WET DISTILLERS GRAINS; IN-VITRO FERMENTATION; CORN GLUTEN FEED; CARCASS CHARACTERISTICS; DAIRY-COWS; CATTLE; DIETS; FORAGE; REQUIREMENTS; METABOLISM AB Roughage is typically mechanically processed to increase digestibility and improve handling and mixing characteristics in beef cattle finishing diets. Roughage is fed to promote ruminal health and decrease digestive upset, but inclusion in finishing diets is limited due to the cost per unit of energy. Rumination behavior may be a means to standardize roughage in beef cattle finishing diets, and increasing particle size of roughage may allow a decrease in roughage inclusion without sacrificing animal performance. Therefore, the objectives of this study were to quantify rumination time for a finishing beef animal and to evaluate the effects of corn stalk (CS) inclusion rate and particle size on rumination behavior, animal performance, and carcass characteristics. Fifty-one individually fed steers (385 +/- 3.6 kg initial BW) were used in a randomized complete block design feeding study. Corn stalks were passed through a tub grinder equipped with a 7.62-cm screen once to generate longgrind CS (LG-CS) or twice to generate short-grind CS (SG-CS). Dietary treatments were based on steam-flaked corn and included, on a DM basis, 30% wet corn gluten feed (WCGF) with 5% SG-CS (5SG), 30% WCGF with 5% LG-CS (5LG), and 25% WCGF with 10% SG-CS (10SG). The Penn State Particle Separator was used to separate ingredients and treatment diets and to estimate physically effective NDF (peNDF). On d 70, each steer was fitted with a collar (HR Tag; SCR Dairy, Netanya, Isreal), which continuously measured rumination minutes via a sensory microphone. Long-grind CS contained more (P < 0.01) peNDF than SG-CS, and the 10SG diet contained more (P = 0.03) peNDF than the 5LG and 5SG diets. Dry matter intake was greatest (P = 0.03) for steers consuming 5LG and least for steers consuming 10SG, with cattle consuming 5SG being intermediate. Carcass-adjusted ADG and G: F were greatest (P = 0.03) for steers consuming 5LG and 5SG compared with steers consuming 10SG. Hot carcass weight tended (P = 0.10) to be greatest for steers consuming 5LG and least for steers consuming 10SG, with steers consuming 5SG being intermediate. Dressing percent was greater (P <= 0.01) for steers consuming 5LG and 5SG than for steers consuming 10SG. A significant interaction (P < 0.01) occurred for rumination minutes x day. Rumination (min/day) were greatest (P = 0.01) for steers consuming 10SG followed by steers consuming 5LG and was lowest for steers consuming 5SG. Increasing particle size of roughage may be a means to decrease roughage inclusion rate while maintaining rumination and performance. C1 [Gentry, W. W.; Weiss, C. P.; Meredith, C. M.; McCollum, F. T.; Jennings, J. S.] Texas A&M AgriLife Res & Extens Serv, Amarillo, TX 79106 USA. [Cole, N. A.] USDA ARS, Conservat & Prod Res Lab, Bushland, TX 79012 USA. RP Jennings, JS (reprint author), Texas A&M AgriLife Res & Extens Serv, Amarillo, TX 79106 USA. EM jenny.jennings@ag.tamu.edu FU USDA-ARS; Texas A&M AgriLife Research, Amarillo; Texas A&M University State Initiative Funds for Distillers Grains Research FX This experiment was supported, in part, by a cooperative agreement between the USDA-ARS and Texas A&M AgriLife Research, Amarillo. The mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The authors thank the Texas A&M University State Initiative Funds for Distillers Grains Research for supporting this research. NR 23 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD NOV PY 2016 VL 94 IS 11 BP 4759 EP 4770 DI 10.2527/jas2016-0734 PG 12 WC Agriculture, Dairy & Animal Science SC Agriculture GA EI6YO UT WOS:000392643800027 PM 27898930 ER PT J AU Davis, ME Lancaster, PA Rutledge, JJ Cundiff, LV AF Davis, M. E. Lancaster, P. A. Rutledge, J. J. Cundiff, L. V. TI Life cycle efficiency of beef production: VIII. Relationship between residual feed intake of heifers and subsequent cow efficiency ratios SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE cattle; feed efficiency; lifetime production; residual feed intake; residual gain ID GENETIC-ENVIRONMENTAL INTERACTIONS; CATTLE; TRAITS; LACTATION; PROGENY; PERFORMANCE; CONSUMPTION; THICKNESS AB Data were collected from 1953 through 1980 from identical and fraternal twin beef and dairy females born in 1953, 1954, 1959, 1964, and 1969, and from crossbred females born as singles in 1974, and their progeny. Numbers of dams that weaned at least 1 calf and were included in the first analysis were 37, 45, and 56 in the 1964, 1969, and 1974 data sets, respectively. Respective numbers of dams that weaned 3 calves and were included in a second analysis were 6, 8, 8, 22, 33, and 33 in the 1953, 1954, 1959, 1964, 1969, and 1974 experiments. Individual feed consumption was measured at 28-d intervals from the time females were placed on the experiment until 3 calves were weaned or the dams had reached 5 yr of age. Residual feed intake (RFI) and residual gain (RG) of the heifers that subsequently became the dams in this study were determined based on ADG and DMI from 240 d of age to first calving. Various measures of cow efficiency were calculated on either a life cycle or actual lifetime basis using ratios of progeny and dam weight outputs to progeny and dam feed inputs. Residual feed intake was phenotypically independent of ADG and metabolic midweight (MMW), whereas the correlation between RFI and DMI was positive and highly significant (r = 0.67; P < 0.0001). Residual gain was highly correlated with ADG (r = 0.75; P < 0.0001) and had near 0 correlations with DMI and MMW. Correlations indicated that heifers that ate less and had smaller metabolic midweights from 240 d of age to first calving had superior efficiency ratios as cows. Residual feed intake was not significantly correlated with age at puberty, age at calving, or milk production. Results of this study do not indicate any serious antagonisms of postweaning heifer RFI with subsequent cow and progeny performance traits or with life cycle or actual lifetime cow efficiency. In addition, selection for increased RG would result in earlier ages at calving, but would also tend to result in taller and heavier cows. C1 [Davis, M. E.] Ohio State Univ, Dept Anim Sci, Columbus, OH 43210 USA. [Lancaster, P. A.] Missouri State Univ, Darr Sch Agr, Springfield, MO 65897 USA. [Rutledge, J. J.] Univ Wisconsin, Dept Anim Sci, Madison, WI 53706 USA. [Cundiff, L. V.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Davis, ME (reprint author), Ohio State Univ, Dept Anim Sci, Columbus, OH 43210 USA. EM davis.28@osu.edu NR 36 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD NOV PY 2016 VL 94 IS 11 BP 4860 EP 4871 DI 10.2527/jas2016-0690 PG 12 WC Agriculture, Dairy & Animal Science SC Agriculture GA EI6YO UT WOS:000392643800036 PM 27898944 ER PT J AU Palazzini, JM Dunlap, CA Bowman, MJ Chulze, SN AF Palazzini, Juan M. Dunlap, Christopher A. Bowman, Michael J. Chulze, Sofia N. TI Bacillus velezensis RC 218 as a biocontrol agent to reduce Fusarium head blight and deoxynivalenol accumulation: Genome sequencing and secondary metabolite cluster profiles SO MICROBIOLOGICAL RESEARCH LA English DT Article DE Bacillus velezensis; FHB biocontrol; Genome sequencing; Secondary metabolite production; Lantibiotic ericin ID AMYLOLIQUEFACIENS FZB42; BIOLOGICAL-CONTROL; GIBBERELLA-ZEAE; BIOSYNTHETIC GENE; PLANT-PATHOGENS; WHEAT; GRAMINEARUM; RESISTANCE; SELECTION; SUBTILIS AB Bacillus subtilis RC 218 was originally isolated from wheat anthers as a potential antagonist of Fusarium graminearum, the causal agent of Fusarium head blight (FHB). It was demonstrated to have antagonist activity against the plant pathogen under in vitro and greenhouse assays. The current study extends characterizing B. subtilis RC 218 with a field study and genome sequencing. The field study demonstrated that B. subtilis RC 218 could reduce disease severity and the associated mycotoxin (deoxynivalenol) accumulation, under field conditions. The genome sequencing allowed us to accurately determine the taxonomy of the strain using a phylogenomic approach, which places it in the Bacillus velezensis Glade. In addition, the draft genome allowed us to use bioinformatics to mine the genome for potential metabolites. The genome mining allowed us to identify 9 active secondary metabolites conserved. by all B. velezensis strains and one additional secondary metabolite, the lantibiotic ericin, which is unique to this strain. This study represents the first confirmed production of ericin by a B. velezensis strain. The genome also allowed us to do a comparative genomics with its closest relatives and compare the secondary metabolite production of the publically available B. velezensis genomes. The results showed that the diversity in secondary metabolites of strains in the B. velezensis Glade is driven by strains making different antibacterials. (C) 2016 Elsevier GmbH. All rights reserved. C1 [Palazzini, Juan M.; Chulze, Sofia N.] Univ Nacl Rio Cuarto, Dept Microbiol & Immunol, Ruta Nacl 36 Km 601, Cordoba, Argentina. [Dunlap, Christopher A.] ARS, Crop Bioprotect, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL USA. [Bowman, Michael J.] ARS, Bioenergy Res Units, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL USA. RP Chulze, SN (reprint author), Univ Nacl Rio Cuarto, Dept Microbiol & Immunol, Ruta Nacl 36 Km 601, Cordoba, Argentina. EM schulze@exa.unrc.edu.ar FU National Agency for Science and Technology Innovation from Argentina [PICT 2013-2876, PICT 1436/12]; CONICET-Argentina [112-201101-00297] FX The authors would like to thank Heather Walker for expert technical assistance. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. The mention of firm names or trade products does not imply that they are endorsed or recommended by the USDA over other firms or similar products not mentioned. USDA is an equal opportunity provider and employer. This work was supported by grants from The National Agency for Science and Technology Innovation from Argentina PICT 2013-2876 and PICT 1436/12, grant from CONICET-Argentina 112-201101-00297. NR 52 TC 1 Z9 1 U1 11 U2 11 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 0944-5013 J9 MICROBIOL RES JI Microbiol. Res. PD NOV PY 2016 VL 192 BP 30 EP 36 DI 10.1016/j.micres.2016.06.002 PG 7 WC Microbiology SC Microbiology GA EI5VA UT WOS:000392563200004 PM 27664721 ER PT J AU Alzan, HF Knowles, DP Suarez, CE AF Alzan, Heba F. Knowles, Donald P. Suarez, Carlos E. TI Comparative Bioinformatics Analysis of Transcription Factor Genes Indicates Conservation of Key Regulatory Domains among Babesia bovis, Babesia microti, and Theileria equi SO PLOS NEGLECTED TROPICAL DISEASES LA English DT Review ID DNA-BINDING PROTEINS; PLASMODIUM-FALCIPARUM; MALARIA PARASITES; SEXUAL STAGES; LIFE-CYCLE; BOX; FAMILY; ARABIDOPSIS; COMMITMENT; EVOLUTION AB Apicomplexa tick-borne hemoparasites, including Babesia bovis, Babesia microti, and Theileria equi are responsible for bovine and human babesiosis and equine theileriosis, respectively. These parasites of vast medical, epidemiological, and economic impact have complex life cycles in their vertebrate and tick hosts. Large gaps in knowledge concerning the mechanisms used by these parasites for gene regulation remain. Regulatory genes coding for DNA binding proteins such as members of the Api-AP2, HMG, and Myb families are known to play crucial roles as transcription factors. Although the repertoire of Api-AP2 has been defined and a HMG gene was previously identified in the B. bovis genome, these regulatory genes have not been described in detail in B. microti and T. equi. In this study, comparative bioinformatics was used to: (i) identify and map genes encoding for these transcription factors among three parasites' genomes; (ii) identify a previously unreported HMG gene in B. microti; (iii) define a repertoire of eight conserved Myb genes; and (iv) identify AP2 correlates among B. bovis and the better-studied Plasmodium parasites. Searching the available transcriptome of B. bovis defined patterns of transcription of these three gene families in B. bovis erythrocyte stage parasites. Sequence comparisons show conservation of functional domains and general architecture in the AP2, Myb, and HMG proteins, which may be significant for the regulation of common critical parasite life cycle transitions in B. bovis, B. microti, and T. equi. A detailed understanding of the role of gene families encoding DNA binding proteins will provide new tools for unraveling regulatory mechanisms involved in B. bovis, B. microti, and T. equi life cycles and environmental adaptive responses and potentially contributes to the development of novel convergent strategies for improved control of babesiosis and equine piroplasmosis. C1 [Alzan, Heba F.] Natl Res Ctr, Parasitol & Anim Dis Dept, Giza, Egypt. [Knowles, Donald P.; Suarez, Carlos E.] WSU, Anim Dis Res Unit, ARS, USDA, Pullman, WA 99163 USA. [Alzan, Heba F.; Knowles, Donald P.; Suarez, Carlos E.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. RP Suarez, CE (reprint author), WSU, Anim Dis Res Unit, ARS, USDA, Pullman, WA 99163 USA.; Suarez, CE (reprint author), Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. EM ces@vetmed.wsu.edu FU United States Department of Agriculture Research Service Current Research Information System [5348-32000-028-00D]; Egyptian government, Ministry of High Education and Scientific Research scholarship FX This work was supported by United States Department of Agriculture Research Service Current Research Information System Project No. 5348-32000-028-00D, and Egyptian government, Ministry of High Education and Scientific Research scholarship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 35 TC 0 Z9 0 U1 2 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1935-2735 J9 PLOS NEGLECT TROP D JI Plos Neglect. Trop. Dis. PD NOV PY 2016 VL 10 IS 11 AR e0004983 DI 10.1371/journal.pntd.0004983 PG 23 WC Infectious Diseases; Parasitology; Tropical Medicine SC Infectious Diseases; Parasitology; Tropical Medicine GA EI0HR UT WOS:000392154400008 PM 27832060 ER PT J AU Kondo, M Hohl, B Han, S Branas, C AF Kondo, Michelle Hohl, Bernadette Han, SeungHoon Branas, Charles TI Effects of greening and community reuse of vacant lots on crime SO URBAN STUDIES LA English DT Article DE community gardens; crime; difference-in-differences; greening; urban health ID NEW-YORK-CITY; BROKEN WINDOWS; HEALTH; BALTIMORE; NEIGHBORHOODS; BUILDINGS; DISORDER; DISEASE; IMPACT; SAFETY AB The Youngstown Neighborhood Development Corporation initiated a Lots of Green' programme to reuse vacant land in 2010. We performed a difference-in-differences analysis of the effects of this programme on crime in and around newly treated lots, in comparison to crimes in and around randomly selected and matched, untreated vacant lot controls. The effects of two types of vacant lot treatments on crime were tested: a cleaning and greening stabilisation' treatment and a community reuse' treatment mostly involving community gardens. The combined effects of both types of vacant lot treatments were also tested. After adjustment for various sociodemographic factors, linear and Poisson regression models demonstrated statistically significant reductions in all crime classes for at least one lot treatment type. Regression models adjusted for spatial autocorrelation found the most consistent significant reductions in burglaries around stabilisation lots, and in assaults around community reuse lots. Spill-over crime reduction effects were found in contiguous areas around newly treated lots. Significant increases in motor vehicle thefts around both types of lots were also found after they had been greened. Community-initiated vacant lot greening may have a greater impact on reducing more serious, violent crimes. C1 [Kondo, Michelle; Han, SeungHoon] USDA Forest Serv, Philadelphia, PA 19072 USA. [Hohl, Bernadette] Rutgers State Univ, New Brunswick, NJ USA. [Han, SeungHoon; Branas, Charles] Univ Penn, Philadelphia, PA USA. RP Kondo, M (reprint author), USDA Forest Serv, Philadelphia, PA 19072 USA.; Kondo, M (reprint author), USDA Forest Serv, Northern Res Stn, 100 North 20th St,Suite 205, Philadelphia, PA 19072 USA. EM michelleckondo@fs.fed.us FU USDA Forest Service, Northern Research Station; National Institutes of Health [10.13039/100000002 R01 AA020331] FX This research was supported by grants from the USDA Forest Service, Northern Research Station and the National Institutes of Health (10.13039/100000002 R01 AA020331). NR 51 TC 2 Z9 2 U1 5 U2 5 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0042-0980 EI 1360-063X J9 URBAN STUD JI Urban Stud. PD NOV PY 2016 VL 53 IS 15 BP 3279 EP 3295 DI 10.1177/0042098015608058 PG 17 WC Environmental Studies; Urban Studies SC Environmental Sciences & Ecology; Urban Studies GA EA8AN UT WOS:000386855600009 ER PT J AU Bian, HY Li, GH Jiao, L Yu, ZH Dui, HQ AF Bian, Huiyang Li, Guanhan Jiao, Liang Yu, Zhihuai Dui, Hongqi TI Enzyme-Assisted Mechanical Fibrillation of Bleached Spruce Kraft Pulp to Produce Well-Dispersed and Uniform-Sized Cellulose Nanofibrils SO BIORESOURCES LA English DT Article DE Cellulose nanofibrils; Mechanical pretreatment; Enzyme hydrolysis; Homogenization; Dispersion ID HIGH-PRESSURE HOMOGENIZATION; MICROFIBRILLATED CELLULOSE; ENDOGLUCANASE TREATMENT; HYDROLYSIS; WOOD; MICROFLUIDIZATION; SACCHARIFICATION; PRETREATMENT; NANOCELLULOSE AB Cellulose nanofibrils (CNFs) are bionanomaterials with many promising properties and great potential in composite applications. Herein, well-dispersed and uniform-sized cellulose nanofibrils were successfully obtained from commercially bleached softwood kraft pulp, resulting in yields of 79.15% via enzyme-assisted hydrolysis and subsequent homogenization. Field emission scanning electron microscopy (FE-SEM) confirmed the fiber morphology. The water retention value (WRV) was increased from 107% of original pulp to 1384% of the resulting CNFs, while crystallinity had no significant changes. Fourier transform infrared (FTIR) spectroscopy indicated that enzymes degraded a portion of hemicelluloses and residual lignin. CNFs subjected to enzymatic treatment and homogenization had a less entangled network, larger aspect ratio, and higher transmittance than those from pure mechanical treatment. They were well dispersed in an aqueous solution and uniformly sized in morphology, resolving the present challenge of nanomaterials tendency for agglomeration. These results revealed that the enzyme-assisted process had a remarkable effect on the production of CNFs and made CNFs more appealing for nanotechnology applications and nanomaterial. C1 [Bian, Huiyang; Li, Guanhan; Jiao, Liang; Yu, Zhihuai; Dui, Hongqi] Nanjing Forestry Univ, Jiangsu Prov Key Lab Pulp & Paper Sci & Technol, Nanjing 210037, Jiangsu, Peoples R China. [Bian, Huiyang] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Dui, HQ (reprint author), Nanjing Forestry Univ, Jiangsu Prov Key Lab Pulp & Paper Sci & Technol, Nanjing 210037, Jiangsu, Peoples R China. EM hgdhq@nifit.edu.cn FU State Forestry Administration, China [2015-4-54]; National Natural Science Foundation of China, China [31470599]; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), China FX The authors are grateful for the support of the State Forestry Administration (Project No. 2015-4-54), China, and the National Natural Science Foundation of China (Project No. 31470599), China. This work also supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), China. NR 36 TC 0 Z9 0 U1 6 U2 6 PU NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI PI RALEIGH PA CAMPUS BOX 8005, RALEIGH, NC 27695-8005 USA SN 1930-2126 J9 BIORESOURCES JI BioResources PD NOV PY 2016 VL 11 IS 4 BP 10483 EP 10496 DI 10.15376/biores.11.4.10483-10496 PG 14 WC Materials Science, Paper & Wood SC Materials Science GA EH5GT UT WOS:000391801300173 ER PT J AU Fissore, C Jurgensen, MF Pickens, J Miller, C Page-Dumroese, D Giardina, CP AF Fissore, Cinzia Jurgensen, Martin F. Pickens, James Miller, Chris Page-Dumroese, Deborah Giardina, Christian P. TI Role of soil texture, clay mineralogy, location, and temperature in coarse wood decomposition-a mesocosm experiment SO ECOSPHERE LA English DT Article DE aspen wood stakes; clay mineral assemblages; kaolinite; mesocosm incubation; montmorillonite; wood decomposition ID ORGANIC-MATTER DYNAMICS; LITTER DECOMPOSITION; ROOT DECOMPOSITION; DECAY-RATES; CARBON; DEBRIS; FOREST; NITROGEN; TURNOVER; RESPIRATION AB Of all the major pools of terrestrial carbon (C), the dynamics of coarse woody debris (CWD) are the least understood. In contrast to soils and living vegetation, the study of CWD has rarely relied on ex situ methods for elaborating controls on decomposition rates. In this study, we report on a mesocosm incubation experiment examining how clay amount (8%, 16%, and 24% clay), clay type (soil reconstructed with kaolinite vs. montmorillonite), wood placement (on litter layer surface, at the litter layer-soil interface, buried in the mineral soil), and laboratory incubation temperature (10 degrees, 20 degrees, or 30 degrees C) control decomposition rates of highly standardized stakes and blocks of coarse aspen wood. Clay type effect was pronounced, with wood decomposing more quickly in kaolinite-than in montmorillonite-amended soils, perhaps due to a combined effect of moisture and microbial access to the substrate. Clay amount had only very limited effect on wood decomposition, which was a function of contact with the mineral soil (Surface < Interface < Mineral), perhaps due to greater contact with the decomposer community. Temperature effects were significant and dependent on interactions with clay type and wood placement. Effects of temperature on wood decomposition declined as the effects of soil variables increased, suggesting a hierarchy of controls on wood decomposition rates. Both water content and temperature had a strong effect on wood decomposition. Our results highlight that multiple interacting factors likely regulate wood decomposition processes. Multifactorial field experiments are needed to examine the physical, chemical, and biological factors controlling wood decomposition. C1 [Fissore, Cinzia] Whittier Coll, Dept Biol & Environm Sci, Whittier, CA 90608 USA. [Jurgensen, Martin F.; Pickens, James; Miller, Chris] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. [Page-Dumroese, Deborah] US Forest Serv, USDA, Rocky Mt Res Stn, Moscow, ID 83843 USA. [Giardina, Christian P.] US Forest Serv, USDA, Pacific Southwest Res Stn, Inst Pacific Isl Forestry, Hilo, HI 96720 USA. RP Fissore, C (reprint author), Whittier Coll, Dept Biol & Environm Sci, Whittier, CA 90608 USA. EM cfissore@whittier.edu RI Giardina, Christian/C-3120-2011 OI Giardina, Christian/0000-0002-3431-5073 FU Northern, Rocky Mountain and Pacific Southwest Research Stations of the USDA Forest Service; Clay Mineral Society FX This work was supported by the Northern, Rocky Mountain and Pacific Southwest Research Stations of the USDA Forest Service and by a grant from the Clay Mineral Society. We thank Jennifer Eikenberry, Melissa Porter, and Christa Luokkala for help with the GC analyses and laboratory incubations. The authors have no conflict of interest to declare. CF, MJ, and CG conceived and designed the study; JP and CM conducted statistical tests; and all authors contributed to performing the research, analyzing the data, and writing the manuscript. NR 69 TC 0 Z9 0 U1 9 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD NOV PY 2016 VL 7 IS 11 AR e01605 DI 10.1002/ecs2.1605 PG 13 WC Ecology SC Environmental Sciences & Ecology GA EI1AP UT WOS:000392207600008 ER PT J AU Malone, SL Tulbure, MG Perez-Luque, AJ Assal, TJ Bremer, LL Drucker, DP Hillis, V Varela, S Goulden, ML AF Malone, Sparkle L. Tulbure, Mirela G. Perez-Luque, Antonio J. Assal, Timothy J. Bremer, Leah L. Drucker, Debora P. Hillis, Vicken Varela, Sara Goulden, Michael L. TI Drought resistance across California ecosystems: evaluating changes in carbon dynamics using satellite imagery SO ECOSPHERE LA English DT Article DE carbon-uptake efficiency; drought effects; ecosystem resistance; ecosystem type conversions; primary productivity; water-use efficiency ID WATER-USE EFFICIENCY; NET PRIMARY PRODUCTION; PRECIPITATION-USE EFFICIENCY; GROSS PRIMARY PRODUCTION; WESTERN UNITED-STATES; TERRESTRIAL ECOSYSTEMS; CLIMATE-CHANGE; MODIS; PRODUCTIVITY; VEGETATION AB Drought is a global issue that is exacerbated by climate change and increasing anthropogenic water demands. The recent occurrence of drought in California provides an important opportunity to examine drought response across ecosystem classes (forests, shrublands, grasslands, and wetlands), which is essential to understand how climate influences ecosystem structure and function. We quantified ecosystem resistance to drought by comparing changes in satellite-derived estimates of water-use efficiency (WUE = net primary productivity [NPP]/evapotranspiration [ET]) under normal (i.e., baseline) and drought conditions (Delta WUE = WUE2014 - baseline WUE). With this method, areas with increasing WUE under drought conditions are considered more resilient than systems with declining WUE. Baseline WUE varied across California (0.08 to 3.85 g C/mm H2O) and WUE generally increased under severe drought conditions in 2014. Strong correlations between Delta WUE, precipitation, and leaf area index (LAI) indicate that ecosystems with a lower average LAI (i.e., grasslands) also had greater C-uptake rates when water was limiting and higher rates of carbon-uptake efficiency (CUE = NPP/LAI) under drought conditions. We also found that systems with a baseline WUE <= 0.4 exhibited a decline in WUE under drought conditions, suggesting that a baseline WUE <= 0.4 might be indicative of low drought resistance. Drought severity, precipitation, and WUE were identified as important drivers of shifts in ecosystem classes over the study period. These findings have important implications for understanding climate change effects on primary productivity and C sequestration across ecosystems and how this may influence ecosystem resistance in the future. C1 [Malone, Sparkle L.] US Forest Serv, Rocky Mt Res Stn, 240 West Prospect Rd, Ft Collins, CO 80524 USA. [Tulbure, Mirela G.] Univ New South Wales, Sch Biol Earth & Environm Sci, Sydney, NSW 2052, Australia. [Perez-Luque, Antonio J.] Univ Granada, Andalusian Ctr Environm Res, Andalusian Inst Earth Syst Res, Lab Ecol iEcolab, Ave Mediterraneo S-N, Granada 18006, Spain. [Assal, Timothy J.] US Geol Survey, Ft Collins Sci Ctr, Ft Collins, CO 80526 USA. [Bremer, Leah L.] Stanford Univ, Nat Capital Project, Stanford Woods Inst Environm, 371 Serra Mall, Stanford, CA 94305 USA. [Drucker, Debora P.] Embrapa Informat Agr, Ave Andre Tosello 209,Campus Unicamp, BR-13083886 Campinas, SP, Brazil. [Hillis, Vicken] Univ Calif Davis, Dept Environm Sci & Policy, One Shields Ave, Davis, CA 95616 USA. [Varela, Sara] Leibniz Inst Evolut & Biodivers Sci, Museum Nat Kunde, Invalidenstr 43, D-10115 Berlin, Germany. [Goulden, Michael L.] Univ Calif Irvine, Dept Earth Syst Sci, Irvine, CA 92697 USA. RP Malone, SL (reprint author), US Forest Serv, Rocky Mt Res Stn, 240 West Prospect Rd, Ft Collins, CO 80524 USA. EM sparklelmalone@fs.fed.us OI Assal, Timothy/0000-0001-6342-2954 FU National Science Foundation at the National Center for Ecological Analysis and Synthesis (NCEAS), a center - University of California, Santa Barbara [OCI-1216894]; State of California; NCEAS; MICINN (Spanish Government) [PTA 2011-6322-I]; USAID; U.S. Department of State through the Sustainable landscapes Brazil program; Australian Research Council [DE140101608] FX The authors would like to acknowledge the excellent support provided by the Open Science for Synthesis-2014 training (supported by the National Science Foundation under Grant No. OCI-1216894) at the National Center for Ecological Analysis and Synthesis (NCEAS), a center funded by the University of California, Santa Barbara, and the State of California. The authors would like to thank OSS instructors Nancy Baron, Ben Bolker, Stephanie Hampton, Matthew Jones, Karthik Ram, Mark Schildhauer, and the participants of the OSS training. The authors would also like to thank the Goulden Lab at the University of California Irvines. A. J. Perez-Luque acknowledges funding received by NCEAS and to MICINN (Spanish Government) for the PTA 2011-6322-I contract; D. P. Drucker acknowledges support from the USAID and the U.S. Department of State through the Sustainable landscapes Brazil program; and M.G. Tulbure acknowledges funding from the Australian Research Council Early Career Researcher Award (DE140101608). Any opinions, findings, conclusions, or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the NCEAS or author affiliations. NR 70 TC 0 Z9 0 U1 7 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD NOV PY 2016 VL 7 IS 11 AR e01561 DI 10.1002/ecs2.1561 PG 19 WC Ecology SC Environmental Sciences & Ecology GA EI1AP UT WOS:000392207600018 ER PT J AU Massie, MH Wilson, TM Morzillo, AT Henderson, EB AF Massie, Margaret H. Wilson, Todd M. Morzillo, Anita T. Henderson, Emilie B. TI Natural areas as a basis for assessing ecosystem vulnerability to climate change SO ECOSPHERE LA English DT Article DE climate change; climate envelope; climate vulnerability; landscape monitoring; natural areas network; random forest ID WESTERN UNITED-STATES; SPECIES DISTRIBUTION; PROTECTED AREAS; RANDOM FORESTS; MODEL; UNCERTAINTIES; BIOCLIMATE; BIODIVERSITY; VEGETATION; FORECASTS AB There are more than 580 natural areas in Oregon and Washington managed by 20 federal, state, local, and private agencies and organizations. This natural areas network is unparalleled in its representation of the diverse ecosystems found in the Pacific Northwest, and could prove useful for monitoring long-term ecological responses to climate change. Our objectives were to (1) evaluate potential effects of climate change on these natural areas and (2) develop strategies for selecting and prioritizing sites for long-term monitoring. Bioclimatic and Random Forest modeling were used to identify subsets of natural areas to prioritize for long-term monitoring efforts based on the current and projected (2020s, 2050s, 2080s) outputs from 13 future climate models. Projection consensus suggest some of the largest effects of climate change on natural areas may be the result of a substantial range increase in suitable climate for warmer-adapted forest types coupled with a reduction in habitat for cooler-adapted forest types. We identify four strategies that could be used for prioritizing sites and help manage and protect biodiversity in the Pacific Northwest, especially given uncertainty over climate change effects. C1 [Massie, Margaret H.] Oregon State Univ, Dept Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA. [Wilson, Todd M.] US Forest Serv, Forestry Sci Lab, USDA, Pacific Northwest Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. [Morzillo, Anita T.] Univ Connecticut, Dept Nat Resources & Environm, Storrs, CT 06269 USA. [Henderson, Emilie B.] Inst Nat Resources, POB 751, Portland, OR 97207 USA. RP Morzillo, AT (reprint author), Univ Connecticut, Dept Nat Resources & Environm, Storrs, CT 06269 USA. EM anita.morzillo@uconn.edu FU Bureau of Land Management; Oregon State Office; USDA Forest Service; Pacific Northwest Research Station; Oregon State University FX Funding for this project was provided by the Bureau of Land Management, Oregon State Office, the USDA Forest Service, Pacific Northwest Research Station, and Oregon State University. NR 75 TC 0 Z9 0 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD NOV PY 2016 VL 7 IS 11 AR e01563 DI 10.1002/ecs2.1563 PG 17 WC Ecology SC Environmental Sciences & Ecology GA EI1AP UT WOS:000392207600019 ER PT J AU Munoz-Erickson, TA Campbell, LK Childers, DL Grove, JM Iwaniec, DM Pickett, STA Romolini, M Svendsen, ES AF Munoz-Erickson, Tischa A. Campbell, Lindsay K. Childers, Daniel L. Grove, J. Morgan Iwaniec, David M. Pickett, Steward T. A. Romolini, Michele Svendsen, Erika S. TI Demystifying governance and its role for transitions in urban social-ecological systems SO ECOSPHERE LA English DT Article DE governance; power; social networks; social-ecological systems; sustainability transitions; urban ID SUSTAINABLE DEVELOPMENT; MANAGEMENT; CITIES; CITY; CHALLENGES; RESILIENCE; NETWORKS; PATHWAYS; POLICY AB Governance is key to sustainable urban transitions. Governance is a system of social, power, and decision-making processes that acts as a key driver of resource allocation and use, yet ecologists-even urban ecologists-seldom consider governance concepts in their work. Transitions to more sustainable futures are becoming increasingly important to the management of many ecosystems and landscapes, and particularly so for urban systems. We briefly identify and synthesize important governance dimensions of urban sustainability transitions, using illustrations from cities in which long-term social-ecological governance research is underway. This article concludes with a call to ecologists who are interested in environmental stewardship, and to urban ecologists in particular, to consider the role of governance as a driver in the dynamics of the systems they study. C1 [Munoz-Erickson, Tischa A.] US Forest Serv, Int Inst Trop Forestry, USDA, 1201 Calle Ceiba, Rio Piedras, PR 00926 USA. [Campbell, Lindsay K.; Svendsen, Erika S.] US Forest Serv, Northern Res Stn, USDA, New York City Urban Field Stn, 431 Walter Reed Rd, Bayside, NY 11359 USA. [Childers, Daniel L.; Iwaniec, David M.] Arizona State Univ, Sch Sustainabil, POB 875502, Tempe, AZ 85287 USA. [Grove, J. Morgan] Arizona State Univ, Northern Res Stn, USDA, Suite 350,5523 Res Pk Dr, Baltimore, MD 21228 USA. [Pickett, Steward T. A.] Cary Inst Ecosyst Studies, 2801 Sharon Turnpike,Box AB, Millbrook, NY 12545 USA. [Romolini, Michele] Loyola Marymount Univ, Ctr Urban Resilience, 1 LMU Dr,113 Res Annex, Los Angeles, CA 90045 USA. RP Munoz-Erickson, TA (reprint author), US Forest Serv, Int Inst Trop Forestry, USDA, 1201 Calle Ceiba, Rio Piedras, PR 00926 USA. EM tamunozerickson@fs.fed.us OI Pickett, Steward/0000-0002-1899-976X FU National Science Foundation (NSF) Urban Sustainability Research Coordination Network (RCN) [1140070]; USDA Forest Service; International Institute of Tropical Forestry and Northern Research Station; NSF [0948507, 1444755]; NSF DA [1027188]; NSF DEB Grant [1027188] FX This work was made possible by the National Science Foundation (NSF) Urban Sustainability Research Coordination Network (RCN) under Grant number 1140070. Other sources of support include the USDA Forest Service, International Institute of Tropical Forestry and Northern Research Station, and the NSF under Grant number 0948507 (Urban Long Term Research Area Exploratory) and the Grant number 1444755 (Urban Resilience to Extreme Weather-related Events Sustainability Research Network). Daniel L. Childers and David Iwaniec received additional support from NSF Grant number 1027188 (Central Arizona-Phoenix Long-Term Ecological Research Program). The contributions of J. Morgan Grove, Michelle Romolini, and Steward T.A. Pickett were additionally supported by NSF DEB Grant number 1027188 (Baltimore Ecosystem Study LTER). Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the view of the National Science Foundation (NSF) and the United States Department of Agriculture (USDA). NR 57 TC 0 Z9 0 U1 6 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD NOV PY 2016 VL 7 IS 11 AR e01564 DI 10.1002/ecs2.1564 PG 11 WC Ecology SC Environmental Sciences & Ecology GA EI1AP UT WOS:000392207600022 ER PT J AU Stephens, SL Miller, JD Collins, BM North, MP Keane, JJ Roberts, SL AF Stephens, Scott L. Miller, Jay D. Collins, Brandon M. North, Malcolm P. Keane, John J. Roberts, Susan L. TI Wildfire impacts on California spotted owl nesting habitat in the Sierra Nevada SO ECOSPHERE LA English DT Article DE coarse filter; conservation; fine-filter; Jeffrey pine; mixed conifer forests; ponderosa pine; prescribed fire; restoration; wildfire ID MIXED-CONIFER FORESTS; FUEL-TREATMENT EFFECTIVENESS; YOSEMITE-NATIONAL-PARK; WESTERN UNITED-STATES; FIRE-PRONE FORESTS; CLIMATE-CHANGE; NORTHWESTERN CALIFORNIA; REPRODUCTIVE SUCCESS; SOUTHERN CASCADES; WILDERNESS AREAS AB California spotted owls (CSOs) (Strix occidentalis occidentalis) have received significant conservation attention beginning with the U.S. Forest Service interim management guidelines in 1992. The most commonly reported forest habitat feature for successful nesting habitat of CSO is canopy cover >70%. Loss and degradation of Sierra Nevada CSO habitat, however, has been a growing concern, initially from commercial tree harvesting and, more recently, from wildfire. This study examined trends in wildfire impacts on potential nesting habitat of the CSO and discusses different management approaches that might lead to the conservation of CSO in fire-dependent forests. A total of 85,046 ha of CSO potential nesting habitat was burned by fire that resulted in >= 50% tree basal area (BA) mortality, reducing canopy cover on average to <25%, during 2000-2014; this included 2.7%, 12.3%, and 7.6% of dense red fir (Abies magnifica), eastside pine, and westside forests, respectively. Based on regression predictions, within the next 75 yr, the cumulative amount of nesting habitat burned at >= 50% tree basal area mortality will exceed the total existing habitat. Four management strategies are discussed that could enhance the conservation of the CSO: (1) increased fire suppression, (2) strategically reducing fire hazards using mechanical treatments and/or prescribed fire, (3) increasing the amount of managed wildfire in CSO habitat, and (4) developing a landscape strategy that uses historical forest structure information to identify areas where high-canopy cover forests are more sustainable. Our estimates of how moderate-and high-severity fire may affect forests into the future poise a substantial threat to CSO persistence. More comprehensive forest restoration activities may be needed in CSO habitat to avoid significant losses of older forests. C1 [Stephens, Scott L.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Div Ecosyst Sci, 130 Mulford Hall, Berkeley, CA 94720 USA. [Miller, Jay D.] US Forest Serv, USDA, Pacific Southwest Reg Fire & Aviat Management, Mcclellan, CA 95652 USA. [Collins, Brandon M.; North, Malcolm P.; Keane, John J.] US Forest Serv, Pacific Southwest Res Stn, USDA, Davis, CA 95618 USA. [Collins, Brandon M.] Univ Calif Berkeley, Ctr Fire Res & Outreach, Berkeley, CA 94720 USA. [Roberts, Susan L.] Univ Calif, Sierra Nevada Res Stn, 7799 Chilnualna Falls Rd, Wawona, CA 95389 USA. RP Stephens, SL (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, Div Ecosyst Sci, 130 Mulford Hall, Berkeley, CA 94720 USA. EM sstephens@berkeley.edu FU Pacific Southwest Region of the U.S. Forest Service FX We wish to thank R. Gerrard for the CSO range map and J. Baldwin for help with the statistics. We appreciate the comments from three reviewers that improved the manuscript. This work was partially funded by the Pacific Southwest Region of the U.S. Forest Service. NR 129 TC 2 Z9 2 U1 5 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD NOV PY 2016 VL 7 IS 11 AR e01478 DI 10.1002/ecs2.1478 PG 21 WC Ecology SC Environmental Sciences & Ecology GA EI1AP UT WOS:000392207600032 ER PT J AU Wang, SG Ma, YP Wan, CB Hse, CY Shupe, TF Wang, YJ Wang, CM AF Wang, Shuguang Ma, Yongpeng Wan, Chengbin Hse, Chungyun Shupe, Todd F. Wang, Yujun Wang, Changming TI Immunolocalization of Endogenous Indole-3-Acetic Acid and Abscisic Acid in the Shoot Internodes of Fargesia yunnanensis Bamboo during Development SO JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE LA English DT Article DE bamboo shoot; internode elongation; endogenous hormone; immunolabeling ID ADVENTITIOUS ROOT-FORMATION; DEVELOPING APPLE FRUIT; PRIMARY PHLOEM FIBERS; ASCORBIC-ACID; VASCULAR DIFFERENTIATION; SIGNAL-TRANSDUCTION; AUXIN BIOSYNTHESIS; QUIESCENT CENTER; CROSS-TALK; ARABIDOPSIS AB The Bambusoideae subfamily includes the fastest-growing plants worldwide, as a consequence of fast internode elongation. However, few studies have evaluated the temporal and spatial distribution of endogenous hormones during internode elongation. In this paper, endogenous indole-3-acetic acid (IAA) and abscisic acid (ABA) were detected in different developmental internodes during shoot elongation by immunolocalization. Immunohistochemistry showed that IAA was mainly present in the shoot apex, leaf sheath primordia, parenchymal cells, and vascular tissues. During internode elongation and maturation, the IAA signals decreased significantly and then increased slightly, with the weakest signals observed in the rapidly elongating internode. Based on immunogold localization, most IAA signals were detected in the cytoplasm and nuclei of both parenchymal and fiber cells, and few signals were detected in cell walls in the unelongated and elongating internodes. After the completion of internode elongation, additional IAA signals were detected in the secondary walls of both parenchymal and fiber cells. Immunohistochemical localization of ABA showed that ABA signals decreased with internode elongation and maturation, with the weakest signal observed in the internodes of 3-month-old shoots. In addition, few ABA signals were detected in the shoot apex. The strongest IAA and ABA signals in unelongated internodes suggested that both hormones participated in the mediation of internode differentiation but not in the rapid elongation. Moreover, IAA was involved in secondary cell wall deposition. C1 [Wang, Shuguang; Wang, Changming] Southwest Forestry Univ, Coll Life Sci, Kunming 650224, Yunnan, Peoples R China. [Ma, Yongpeng] Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China. [Wan, Chengbin] Southwest Forestry Univ, Coll Foreign Languages, Kunming 650224, Yunnan, Peoples R China. [Hse, Chungyun] US Forest Serv, Southern Res Stn, USDA, Pineville, LA 71360 USA. [Shupe, Todd F.] Louisiana State Univ, Sch Renewable Natl Resources, Ctr Agr, Baton Rouge, LA 70803 USA. [Wang, Yujun] Nanjing Forestry Univ, Bamboo Res Inst, Nanjing 210037, Jiangsu, Peoples R China. RP Wang, CM (reprint author), Southwest Forestry Univ, Coll Life Sci, Kunming 650224, Yunnan, Peoples R China. EM 253692357@qq.com FU Advanced and Characteristic Key Biological Disciplines of Yunnan Province [50097505]; Science Foundation of Yunnan Province [2015FB150]; China National Science Foundation [31560196] FX This research was fully funded by the Advanced and Characteristic Key Biological Disciplines of Yunnan Province (50097505), the Science Foundation of Yunnan Province (2015FB150), and the China National Science Foundation (31560196). NR 70 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0003-1062 EI 2327-9788 J9 J AM SOC HORTIC SCI JI J. Am. Soc. Hortic. Sci. PD NOV PY 2016 VL 141 IS 6 BP 563 EP 572 DI 10.21273/JASHS03814-16 PG 10 WC Horticulture SC Agriculture GA EH6FN UT WOS:000391868300004 ER PT J AU Chavez, DJ Beckman, TG Chaparro, JX AF Chavez, Dario J. Beckman, Thomas G. Chaparro, Jose X. TI Identifying the North American Plum Species Phylogenetic Signal Using Nuclear, Mitochondrial, and Chloroplast DNA Markers SO JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE LA English DT Article DE cpDNA; haploids; ITS; nuclear genes; phylogenetic analyses; PICS; section Prunocerasus; single-copy gene; SSRs ID PRUNUS SECT. PRUNOCERASUS; GENETIC DIVERSITY; NONCODING REGIONS; UNIVERSAL PRIMERS; ROSACEAE; SEQUENCES; TORTOISE; MODEL; HARE; IDENTIFICATION AB Prunus phylogeny has been extensively studied using chloroplast DNA (cpDNA) sequences. Chloroplast DNA has a slow rate of evolution, which is beneficial to determine species relationships at a deeper level. The chloroplast-based phylogenies have a limitation due to the transfer of this organelle by interspecific hybridization. This creates difficulties when studying species relationships. Interspecific hybrids in Prunus occur naturally and have been reported, which creates a problem when using cpDNA-based phylogenies to determine species relationships. The main goal of this project was to identify nuclear gene regions that could provide an improved phylogenetic signal at the species level in Prunus. A total of 11 species in Prunus and within section Prunocerasus were used. Two peach (Prunus persica) haploids were used to test the reliability of the molecular markers developed in this project to amplify single-copy genes. A total of 33 major genes associated with vernalization response, 16 with tree architecture, and 3 with isozymes, were tested. Similarly, 41 simple sequence repeat (SSR) markers, seven cpDNA regions, and the internal transcribed spacer (ITS) region, were used. Multiple gene regions were identified and provided the greatest number of characters, greatest variability, and improved phylogenetic signal at the species level in Prunus section Prunocerasus. Out of those, trnH-psbA, PGI, MAX4, AXR1, LFY, PHYE, and VRN1 are recommended for a phylogenetic analysis with a larger number of taxa. The use of potentially informative characters (PICS) as a measure of how informative a region will be for phylogenetic analyses has been previously reported beneficial in cpDNA regions and it clearly was important in this research. This will allow selecting the region(s), which can be used in phylogenetic studies with higher number of taxa. C1 [Chavez, Dario J.] Univ Georgia, Dept Hort, Griffin Campus, Griffin, GA 30223 USA. [Beckman, Thomas G.] USDA ARS, Southeastern Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. [Chaparro, Jose X.] Univ Florida, Dept Hort Sci, Gainesville, FL 32611 USA. RP Chavez, DJ (reprint author), Univ Georgia, Dept Hort, Griffin Campus, Griffin, GA 30223 USA. EM dchavez@uga.edu FU Germplasm Committee [5306-21000-018-00D] FX We thank Wayne Sherman, Pamela Soltis, Doug Soltis, Matias Kirst, Jim Olmstead, and Kent Perkins (University of Florida) and Dick Okie (USDA-ARS) for their support and knowledge provided for this project. We also thank a large group of collaborators in multiple private and public institutions who helped before, during, and after specimen collections. They also extend their thanks to the USDA Prunus Germplasm Collection, its director and curators, and the Prunus Germplasm Committee for their financial support for the germplasm collection used in this project, USDA-ARS project No. 5306-21000-018-00D. NR 58 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0003-1062 EI 2327-9788 J9 J AM SOC HORTIC SCI JI J. Am. Soc. Hortic. Sci. PD NOV PY 2016 VL 141 IS 6 BP 623 EP + DI 10.21273/JASHS03875-16 PG 114 WC Horticulture SC Agriculture GA EH6FN UT WOS:000391868300011 ER PT J AU Qin, GZ Zhu, Z Wang, WH Cai, JH Chen, Y Li, L Tian, SP AF Qin, Guozheng Zhu, Zhu Wang, Weihao Cai, Jianghua Chen, Yong Li, Li Tian, Shiping TI A Tomato Vacuolar Invertase Inhibitor Mediates Sucrose Metabolism and Influences Fruit Ripening SO PLANT PHYSIOLOGY LA English DT Article ID PLANT DEVELOPMENT; EXPRESSION ANALYSIS; SUGAR COMPOSITION; GENE-EXPRESSION; MESSENGER-RNA; POTATO-TUBERS; ACID; CLONING; PROTEIN; FRUCTOKINASE AB Fruit ripening is a complex process that involves a series of physiological and biochemical changes that ultimately influence fruit quality traits, such as color and flavor. Sugar metabolism is an important factor in ripening, and there is evidence that it influences various aspects of ripening, although the associated mechanism is not well understood. In this study, we identified and analyzed the expression of 36 genes involved in Suc metabolism in ripening tomato (Solanum lycopersicum) fruit. Chromatin immunoprecipitation and gel mobility shift assays indicated that SlVIF, which encodes a vacuolar invertase inhibitor, and SlVI, encoding a vacuolar invertase, are directly regulated by the global fruit ripening regulator RIPENING INHIBITOR (RIN). Moreover, we showed that SlVIF physically interacts with SlVI to control Suc metabolism. Repression of SlVIF by RNA interference delayed tomato fruit ripening, while overexpression of SlVIF accelerated ripening, with concomitant changes in lycopene production and ethylene biosynthesis. An isobaric tags for relative and absolute quantification-based quantitative proteomic analysis further indicated that the abundance of a set of proteins involved in fruit ripening was altered by suppressing SlVIF expression, including proteins associated with lycopene generation and ethylene synthesis. These findings provide evidence for the role of Suc in promoting fruit ripening and establish that SlVIF contributes to fruit quality and the RIN-mediated ripening regulatory mechanisms, which are of significant agricultural value. C1 [Qin, Guozheng; Zhu, Zhu; Wang, Weihao; Cai, Jianghua; Chen, Yong; Tian, Shiping] Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China. [Wang, Weihao; Cai, Jianghua; Chen, Yong; Tian, Shiping] Univ Chinese Acad Sci, Beijing 100049, Peoples R China. [Li, Li] USDA, Robert W Holley Ctr Agr Hlth, Agr Res Serv, Ithaca, NY 14853 USA. RP Tian, SP (reprint author), Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China.; Tian, SP (reprint author), Univ Chinese Acad Sci, Beijing 100049, Peoples R China. EM tsp@ibcas.ac.cn FU National Natural Science Foundation of China [31530057]; National Basic Research Program of China (973 Program) [2011CB100604]; Youth Innovation Promotion Association CAS FX This work was supported by the National Natural Science Foundation of China (grant no. 31530057 to S.T.), the National Basic Research Program of China (973 Program; grant no. 2011CB100604 to S.T.), and the Youth Innovation Promotion Association CAS (to G.Q.). NR 70 TC 1 Z9 1 U1 23 U2 23 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD NOV PY 2016 VL 172 IS 3 BP 1596 EP 1611 DI 10.1104/pp.16.01269 PG 16 WC Plant Sciences SC Plant Sciences GA EG6QZ UT WOS:000391172300019 PM 27694342 ER PT J AU Cuneo, IF Knipfer, T Brodersen, CR McElrone, AJ AF Cuneo, Italo F. Knipfer, Thorsten Brodersen, Craig R. McElrone, Andrew J. TI Mechanical Failure of Fine Root Cortical Cells Initiates Plant Hydraulic Decline during Drought SO PLANT PHYSIOLOGY LA English DT Article ID RESOLUTION COMPUTED-TOMOGRAPHY; STRESS-INDUCED CAVITATION; IN-VIVO VISUALIZATIONS; X-RAY MICROTOMOGRAPHY; WATER STATUS; VULNERABILITY SEGMENTATION; ARTEMISIA-TRIDENTATA; CENTRIFUGE TECHNIQUE; DESERT SUCCULENTS; INDUCED EMBOLISM AB Root systems perform the crucial task of absorbing water from the soil to meet the demands of a transpiring canopy. Roots are thought to operate like electrical fuses, which break when carrying an excessive load under conditions of drought stress. Yet the exact site and sequence of this dysfunction in roots remain elusive. Using in vivo x-ray computed microtomography, we found that drought-induced mechanical failure (i. e. lacunae formation) in fine root cortical cells is the initial and primary driver of reduced fine root hydraulic conductivity (Lp(r)) under mild to moderate drought stress. Cortical lacunae started forming under mild drought stress (-0.6 MPa Psi stem), coincided with a dramatic reduction in Lp(r), and preceded root shrinkage or significant xylem embolism. Only under increased drought stress was embolism formation observed in the root xylem, and it appeared first in the fine roots (50% loss of hydraulic conductivity [P-50] reached at -1.8 MPa) and then in older, coarse roots (P-50 = 23.5 MPa). These results suggest that cortical cells in fine roots function like hydraulic fuses that decouple plants from drying soil, thus preserving the hydraulic integrity of the plant's vascular system under early stages of drought stress. Cortical lacunae formation led to permanent structural damage of the root cortex and nonrecoverable Lp(r), pointing to a role in fine root mortality and turnover under drought stress. C1 [Cuneo, Italo F.; Knipfer, Thorsten; McElrone, Andrew J.] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. [Cuneo, Italo F.] Pontificia Univ Catolica Valparaiso, Sch Agron, Quillota, Chile. [Brodersen, Craig R.] Yale Univ, Sch Forestry & Environm Studies, New Haven, CT 06511 USA. [McElrone, Andrew J.] USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. RP McElrone, AJ (reprint author), Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA.; McElrone, AJ (reprint author), USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. EM ajmcelrone@ucdavis.edu OI Cuneo, Italo/0000-0002-8092-0987 FU American Vineyard Foundation; USDA-ARS CRIS funding [5306-21220-004-00]; Katherine Esau Graduate fellowship program; Office of Science, Office of Basic Energy Science, of the U.S. Department of Energy [DE-AC02-05CH11231] FX This work was financially supported by funding from the American Vineyard Foundation to A.J.M. and by USDA-ARS CRIS funding (grant number 5306-21220-004-00). I.F.C. received funding through the Katherine Esau Graduate fellowship program. A.L.S. is supported by the Director, Office of Science, Office of Basic Energy Science, of the U.S. Department of Energy under contract number DE-AC02-05CH11231. NR 75 TC 1 Z9 1 U1 5 U2 5 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD NOV PY 2016 VL 172 IS 3 BP 1669 EP 1678 DI 10.1104/pp.16.00923 PG 10 WC Plant Sciences SC Plant Sciences GA EG6QZ UT WOS:000391172300024 PM 27621427 ER PT J AU Olukolu, BA Bian, Y De Vries, B Tracy, WF Wisser, RJ Holland, JB Balint-Kurti, PJ AF Olukolu, Bode A. Bian, Yang De Vries, Brian Tracy, William F. Wisser, Randall J. Holland, James B. Balint-Kurti, Peter J. TI The Genetics of Leaf Flecking in Maize and Its Relationship to Plant Defense and Disease Resistance SO PLANT PHYSIOLOGY LA English DT Article ID GENOME-WIDE ASSOCIATION; PROGRAMMED CELL-DEATH; ADVANCED INTERCROSS LINE; QUANTITATIVE TRAIT LOCI; LESION MIMIC MUTANTS; MAPPING POPULATION; HYPERSENSITIVE RESPONSE; ARABIDOPSIS-THALIANA; OXIDATIVE STRESS; DIVERSITY PANEL AB Physiological leaf spotting, or flecking, is a mild-lesion phenotype observed on the leaves of several commonly used maize (Zea mays) inbred lines and has been anecdotally linked to enhanced broad-spectrum disease resistance. Flecking was assessed in the maize nested association mapping (NAM) population, comprising 4,998 recombinant inbred lines from 25 biparental families, and in an association population, comprising 279 diverse maize inbreds. Joint family linkage analysis was conducted with 7,386 markers in the NAM population. Genome-wide association tests were performed with 26.5 million single-nucleotide polymorphisms (SNPs) in the NAM population and with 246,497 SNPs in the association population, resulting in the identification of 18 and three loci associated with variation in flecking, respectively. Many of the candidate genes colocalizing with associated SNPs are similar to genes that function in plant defense response via cell wall modification, salicylic acid-and jasmonic acid-dependent pathways, redox homeostasis, stress response, and vesicle trafficking/remodeling. Significant positive correlations were found between increased flecking, stronger defense response, increased disease resistance, and increased pest resistance. A nonlinear relationship with total kernel weight also was observed whereby lines with relatively high levels of flecking had, on average, lower total kernel weight. We present evidence suggesting that mild flecking could be used as a selection criterion for breeding programs trying to incorporate broad-spectrum disease resistance. C1 [Olukolu, Bode A.; Balint-Kurti, Peter J.] North Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. [Olukolu, Bode A.; Bian, Yang; Holland, James B.] North Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [De Vries, Brian; Tracy, William F.] Univ Wisconsin, Dept Agron, Madison, WI 53706 USA. [Wisser, Randall J.] Univ Delaware, Dept Plant & Soil Sci, Newark, DE 19716 USA. [Holland, James B.; Balint-Kurti, Peter J.] ARS, USDA, Plant Sci Res Unit, Raleigh, NC 27695 USA. RP Balint-Kurti, PJ (reprint author), North Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA.; Balint-Kurti, PJ (reprint author), ARS, USDA, Plant Sci Res Unit, Raleigh, NC 27695 USA. EM peter.balint-kurti@ars.usda.gov OI Holland, James/0000-0002-4341-9675; Olukolu, Bode/0000-0003-4143-8909; Balint-Kurti, Peter/0000-0002-3916-194X FU U.S. Department of Agriculture-Agricultural Research Service; National Science Foundation [0822495, 1127076, 1238014, 1444503] FX This work was supported by the U.S. Department of Agriculture-Agricultural Research Service and by the National Science Foundation (grant nos. 0822495, 1127076, 1238014, and 1444503). NR 115 TC 0 Z9 0 U1 5 U2 5 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD NOV PY 2016 VL 172 IS 3 BP 1787 EP 1803 DI 10.1104/pp.15.01870 PG 17 WC Plant Sciences SC Plant Sciences GA EG6QZ UT WOS:000391172300033 PM 27670817 ER PT J AU Willett, DS George, J Willett, NS Stelinski, LL Lapointe, SL AF Willett, Denis S. George, Justin Willett, Nora S. Stelinski, Lukasz L. Lapointe, Stephen L. TI Machine Learning for Characterization of Insect Vector Feeding SO PLOS COMPUTATIONAL BIOLOGY LA English DT Article ID CANDIDATUS LIBERIBACTER ASIATICUS; STYLET PENETRATION; DIAPHORINA-CITRI; GREENING DISEASE; RANDOM FORESTS; R-PACKAGE; ALGORITHM; APHIDS; CLASSIFICATION; SOLANACEARUM AB Insects that feed by ingesting plant and animal fluids cause devastating damage to humans, livestock, and agriculture worldwide, primarily by transmitting pathogens of plants and animals. The feeding processes required for successful pathogen transmission by sucking insects can be recorded by monitoring voltage changes across an insect-food source feeding circuit. The output from such monitoring has traditionally been examined manually, a slow and onerous process. We taught a computer program to automatically classify previously described insect feeding patterns involved in transmission of the pathogen causing citrus greening disease. We also show how such analysis contributes to discovery of previously unrecognized feeding states and can be used to characterize plant resistance mechanisms. This advance greatly reduces the time and effort required to analyze insect feeding, and should facilitate developing, screening, and testing of novel intervention strategies to disrupt pathogen transmission affecting agriculture, livestock and human health. C1 [Willett, Denis S.] USDA ARS, Chem Unit, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [George, Justin; Lapointe, Stephen L.] USDA ARS, Subtrop Insects & Hort Res Unit, US Hort Res Lab, Ft Pierce, FL USA. [Willett, Nora S.] Princeton Univ, Dept Comp Sci, Princeton, NJ 08544 USA. [Stelinski, Lukasz L.] Univ Florida, Citrus Res & Educ Ctr, Dept Entomol & Nematol, Lake Alfred, FL USA. RP Willett, DS (reprint author), USDA ARS, Chem Unit, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. EM denis.willett@ars.usda.gov FU Citrus Research and Development Foundation FX Significant funding was provided by the Citrus Research and Development Foundation. http://citrusrdf.org/ The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 61 TC 0 Z9 0 U1 7 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-734X EI 1553-7358 J9 PLOS COMPUT BIOL JI PLoS Comput. Biol. PD NOV PY 2016 VL 12 IS 11 AR e1005158 DI 10.1371/journal.pcbi.1005158 PG 14 WC Biochemical Research Methods; Mathematical & Computational Biology SC Biochemistry & Molecular Biology; Mathematical & Computational Biology GA EG7MC UT WOS:000391230900010 PM 27832081 ER PT J AU Davidson, J Summerfelt, R Barrows, F Gottsacker, B Good, C Fischer, G Summerfelt, S AF Davidson, John Summerfelt, Robert Barrows, Frederic Gottsacker, Brandon Good, Christopher Fischer, Gregory Summerfelt, Steven TI Walleye Sander vitreus performance, water quality, and waste production in replicated recirculation aquaculture systems when feeding a low phosphorus diet without fishmeal versus a traditional fishmeal-based diet SO AQUACULTURAL ENGINEERING LA English DT Article DE Walleye; Recirculating aquaculture systems; Fishmeal-free diet; Aquaculture waste; Poultry meal; Alternate proteins ID ONCORHYNCHUS-MYKISS PERFORMANCE; PLANT PROTEIN SUBSTITUTION; SALMON SALMO-SALAR; GUAR GUM ADDITION; RAINBOW-TROUT; LUCIOPERCA L.; ATLANTIC SALMON; PROXIMATE COMPOSITION; GROWTH-PERFORMANCE; BLOOD PARAMETERS AB Walleye Sander vitreus is a popular sport-and food-fish in areas surrounding the Great Lakes. Walleye are mainly provided as food-fish by limited capture fisheries, but have potential for profitable production to market-size in recirculation aquaculture systems (RAS). Walleye are piscivorous with a supposed requirement for fishmeal in artificial diets, thus little information is available regarding the effects of feeding fishmeal-free diets to walleye. During this study, the health and growth performance of juvenile walleye cultured in RAS were compared between groups fed either a traditional fishmeal-based diet (FM) or a low phosphorous, fishmeal-free (FMF) diet. Water quality and waste production rates resulting from feeding each diet were evaluated. The FM diet contained fishmeal, poultry meal, soybean meal, wheat flour, and blood meal proteins; and the FMF diet used poultry meal, wheat flour, soy protein concentrate, and corn protein concentrate proteins. The only lipid source used in the FM diet was fish oil from menhaden, whereas the FMF diet used menhaden oil and poultry oil. Each diet was formulated with a protein: fat ratio of approximately 42/18. Fish (initial weight 85 g fish(-1)) were cultured in 6 replicated RAS for 9 months, each operated with 99.9% water recycle on a flow basis, a mean system hydraulic retention time of 135 days, and a mean feed loading rate of 3.5 kg feed/m(3) of daily makeup water. At study's end, mean weights standard error of fish fed the FM and FMF diets were 571 +/- 26 and 589 +/- 15 g, respectively (P>0.05). Cumulative survival for both diet treatments was >= 98.5%. Average thermal growth coefficient (TGC), condition factor (CF), and feed conversion ratio (FCR) were similar (P > 0.05) for the FM and FMF diets, respectively: TGC was 0.82 +/- 0.01 and 0.83 +/- 0.02; CF was 1.05 +/- 0.02 and 1.03 +/- 0.02; and FCR was 1.32 +/- 0.02 and 1.27 +/- 0.03. Water color index and UV transmittance values (P < 0.05) indicated slightly clearer water in RAS where the FMF diet was fed. Total nitrogen (TN) was greater (P<0.05) in the culture water of RAS associated with the FM diet; however, TN production per unit feed was similar between treatments; 0.031 +/- 0.010 kg TN/kg feed for the FM diet and 0.030 +/- 0.009 kg TN/kg feed for the FMF diet. Total phosphorous (TP) concentration in the culture water of RAS associated with the FMF diet was 48% of that measured for the FM diet; TP produced per unit feed reflected this trend, 0.107 +/- 0.003 vs. 0.0049 +/- 0.006 kg TP/kg feed for the FM and FMF diets, respectively. Average fillet yield (skin and scales on) of fish harvested at the end of the trial was 47-49% (P>0.05). Whole-body and fillet proximate composition was similar between treatments; however, gonadosomatic index and the ratio of omega 6: 3 fatty acids was greater (P < 0.05) for walleye fed the FMF diet. This was the first study of its kind to report comparable walleye growth when feeding a specially formulated diet devoid of fishmeal and while culturing this species in RAS. Reduced phosphorous discharge resulting from feeding this fishmeal-free diet formulation increases the feasibility of meeting stringent effluent requirements and possibly reduces the capital investment required for waste treatment. (C) 2016 The Authors. Published by Elsevier B.V. C1 [Davidson, John; Good, Christopher; Summerfelt, Steven] Conservat Funds Freshwater Inst, 1098 Turner Rd, Shepherdstown, WV 25443 USA. [Summerfelt, Robert] Iowa State Univ, Dept Nat Resource Ecol & Management, Ames, IA 50011 USA. [Barrows, Frederic] ARS, USDA, Fish Technol Ctr, 4050 Bridger Canyon Rd, Bozeman, MT USA. [Gottsacker, Brandon] North Country Clear Waters LLC, Arcadia, WI 54612 USA. [Fischer, Gregory] Univ Wisconsin, Stevens Point Northern Aquaculture Demonstrat Fac, 36445 State Hwy 13,POB 765, Bayfield, WI 54814 USA. RP Summerfelt, S (reprint author), Conservat Funds Freshwater Inst, 1098 Turner Rd, Shepherdstown, WV 25443 USA. EM s.summerfelt@freshwaterinstitute.org FU North Country Clear Waters, LLC (Arcadia, WI, USA) FX Special thanks to Karen Schroyer, Susan Glenn, and Briana Taylor for water chemistry analysis; to Heather Dailey and Clayton Raines for fish husbandry and technical assistance; and to Dr. Brett Kenney and assistant Susan Slider for product quality analyses. This research was supported by North Country Clear Waters, LLC (Arcadia, WI, USA). All experimental protocols and methods were in compliance with the Animal Welfare Act (9CFR) requirements and were approved by the Freshwater Institute's Institutional Animal Care and Use Committee. Use of trade names does not imply endorsement by North Country Clear Waters or the Conservation Fund's Freshwater Institute. NR 68 TC 0 Z9 0 U1 4 U2 4 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8609 EI 1873-5614 J9 AQUACULT ENG JI Aquac. Eng. PD NOV PY 2016 VL 75 BP 1 EP 13 DI 10.1016/j.aquaeng.2016.09.005 PG 13 WC Agricultural Engineering; Fisheries SC Agriculture; Fisheries GA EG0KM UT WOS:000390721700001 ER PT J AU Longo, M Keller, M dos-Santos, MN Leitold, V Pinage, ER Baccini, A Saatchi, S Nogueira, EM Batistella, M Morton, DC AF Longo, Marcos Keller, Michael dos-Santos, Maiza N. Leitold, Veronika Pinage, Ekena R. Baccini, Alessandro Saatchi, Sassan Nogueira, Euler M. Batistella, Mateus Morton, Douglas C. TI Aboveground biomass variability across intact and degraded forests in the Brazilian Amazon SO GLOBAL BIOGEOCHEMICAL CYCLES LA English DT Article DE Amazon; biomass; forest degradation; airborne lidar; land use change; fire ID TROPICAL FOREST; AIRBORNE LIDAR; CARBON STOCKS; TREE HEIGHT; LOGGED FORESTS; EASTERN AMAZON; SATELLITE DATA; DENSITY MAPS; DEFORESTATION; DYNAMICS AB Deforestation rates have declined in the Brazilian Amazon since 2005, yet degradation from logging, fire, and fragmentation has continued in frontier forests. In this study we quantified the aboveground carbon density (ACD) in intact and degraded forests using the largest data set of integrated forest inventory plots (n = 359) and airborne lidar data (18,000ha) assembled to date for the Brazilian Amazon. We developed statistical models relating inventory ACD estimates to lidar metrics that explained 70% of the variance across forest types. Airborne lidar-ACD estimates for intact forests ranged between 5.0 2.5 and 31.9 10.8kgCm(-2). Degradation carbon losses were large and persistent. Sites that burned multiple times within a decade lost up to 15.0 0.7kgCm(-2) (94%) of ACD. Forests that burned nearly 15years ago had between 4.1 0.5 and 6.8 +/- 0.3kgCm(-2) (22-40%) less ACD than intact forests. Even for low-impact logging disturbances, ACD was between 0.7 +/- 0.3 and 4.4 +/- 0.4kgCm(-2) (4-21%) lower than unlogged forests. Comparing biomass estimates from airborne lidar to existing biomass maps, we found that regional and pantropical products consistently overestimated ACD in degraded forests, underestimated ACD in intact forests, and showed little sensitivity to fires and logging. Fine-scale heterogeneity in ACD across intact and degraded forests highlights the benefits of airborne lidar for carbon mapping. Differences between airborne lidar and regional biomass maps underscore the need to improve and update biomass estimates for dynamic land use frontiers, to better characterize deforestation and degradation carbon emissions for regional carbon budgets and Reduce Emissions from Deforestation and forest Degradation (REDD+). C1 [Longo, Marcos; Keller, Michael; dos-Santos, Maiza N.; Pinage, Ekena R.] Embrapa Agr Informat, Campinas, Brazil. [Keller, Michael] USDA Forest Serv, Int Inst Trop Forestry, Rio Piedras, PR USA. [Keller, Michael; Saatchi, Sassan] CALTECH, Jet Prop Lab, Pasadena, CA USA. [Leitold, Veronika; Morton, Douglas C.] NASA, Goddard Space Flight Ctr, Greenbelt, MD USA. [Pinage, Ekena R.] Univ Technol Sydney, Plant Funct Biol & Climate Change Cluster, Sydney, NSW 2007, Australia. [Baccini, Alessandro] Woods Hole Res Ctr, Falmouth, MA USA. [Nogueira, Euler M.] Natl Inst Res Amazonia, Manaus, Amazonas, Brazil. [Batistella, Mateus] Brazilian Agr Res Corp Embrapa, Brasilia, DF, Brazil. RP Longo, M (reprint author), Embrapa Agr Informat, Campinas, Brazil. EM mdplongo@gmail.com RI Morton, Douglas/D-5044-2012 FU Brazilian National Council for Scientific and Technological Development (CNPq) [151409/2014-5, 407366/2013-0, 457927/2013-5]; Sao Paulo State Research Foundation (FAPESP) [2015/07227-6]; NASA Carbon Monitoring System Program [NASA CMSNNH13AW64I]; Next Generation Ecosystem Experiment-Tropics; U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research FX The airborne lidar and forest inventory data used in this study are publicly available at https://www.paisagenslidar.cnptia.embrapa.br/webgis/, and Landsat chronosequences were obtained through the Earth Resources Observation and Science Center (EROS) from the U.S. Geological Survey (USGS) (http://glovis.usgs.gov/). Plot properties, carbon stocks estimates based on inventory, and airborne lidar are available in Data Set S1; the R scripts are available upon e-mail request (mdplongo@gmail.com). The authors would like to thank Marcos Scaranello, Paul Duffy, and two anonymous reviewers, whose comments and suggestions contributed to greatly improve the manuscript, and Rone Parente, Manoel Vitorino, Cesar Pinheiro, Alfredo Viana, Miguel de Jesus, and Amildo de Jesus from Instituto Floresta Tropical for their work in the forest inventory measurements. Data were acquired by the Sustainable Landscapes Brazil project supported by the Brazilian Agricultural Research Corporation (EMBRAPA), the U.S. Forest Service, and USAID, and the U.S. Department of State. This research has been also supported by the Brazilian National Council for Scientific and Technological Development (CNPq, grants 151409/2014-5, 407366/2013-0, and 457927/2013-5), by the Sao Paulo State Research Foundation (FAPESP, grant 2015/07227-6), and by NASA Carbon Monitoring System Program (NASA CMSNNH13AW64I). M.K. was supported as part of the Next Generation Ecosystem Experiment-Tropics, funded by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research. NR 127 TC 1 Z9 1 U1 9 U2 9 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0886-6236 EI 1944-9224 J9 GLOBAL BIOGEOCHEM CY JI Glob. Biogeochem. Cycle PD NOV PY 2016 VL 30 IS 11 BP 1639 EP 1660 DI 10.1002/2016GB005465 PG 22 WC Environmental Sciences; Geosciences, Multidisciplinary; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Geology; Meteorology & Atmospheric Sciences GA EG1SK UT WOS:000390812300005 ER PT J AU Zhou, ZY Lei, Y Su, D Zhang, HH He, DJ Yang, CH AF Zhou Zhaoyong Lei Yu Su Dong Zhang Haihui He Dongjian Yang, Chenghai TI Detection of moldy core in apples and its symptom types using transmittance spectroscopy SO INTERNATIONAL JOURNAL OF AGRICULTURAL AND BIOLOGICAL ENGINEERING LA English DT Article DE moldy core of apples; transmittance spectrum; wavelet transform; support vector machine; genetic algorithm; symptom types ID ONLINE DETECTION; CLASSIFICATION; BROWNHEART; BRUISE AB A detection method based on transmittance spectroscopy and support vector machine (SVM) was proposed to achieve rapid nondestructive detection of moldy core in apples. A visible to near-infrared (Vis/NIR) spectroradiometer was used for scanning transmittance spectra of 215 apple samples in the wavelength range of 200-1025 nm. Wavelet transform was used to reduce the dimensionality of the spectra and extract wavelet coefficients. Two classification algorithms including artificial neural network (ANN) and SVM were used to develop models whose parameters were optimized by genetic algorithms (GA) for determination of the presence and types of moldy core in apples. Comparisons results of the models showed that the GA-SVM model obtained the optimal result with an accuracy of 96.92% for detecting the presence of moldy core and 81.48% for distinguishing symptom types of the disease. These results indicate that it is feasible to detect moldy core in apples nondestructively and rapidly based on transmittance spectroscopy and that wavelet transform is an effective method for extraction of characteristics from spectra. Moreover, the GA-SVM algorithm in conjunction with Vis/NIR transmittance spectroscopy can accurately achieve fast and nondestructive detection of the presence and types of moldy core in apples. C1 [Zhou Zhaoyong; Lei Yu; Su Dong; Zhang Haihui; He Dongjian] Northwest A&F Univ, Coll Mech & Elect Engn, Yangling 712100, Shaanxi, Peoples R China. [Zhou Zhaoyong] Northwest A&F Univ, Network & Educ Technol Ctr, Yangling 712100, Peoples R China. [Yang, Chenghai] ARS, USDA, College Stn, TX 77845 USA. RP He, DJ (reprint author), Northwest A&F Univ, Coll Mech & Elect Engn, Yangling 712100, Shaanxi, Peoples R China. EM yzz@nwsuaf.edu.cn; ly759059219@163.com; sudong@nwsuaf.edu.cn; zhanghh@nwsuaf.edu.cn; hdj168@nwsuaf.edu.cn; Chenghai.Yang@ars.usda.gov FU National High-tech Research and Development Projects (863) [2013AA10230402]; National Natural Science Foundation of China [61473235]; Major Pilot Projects of the Agro-Tech Extension and Service in Shaanxi [2016XXPT-05] FX The research was supported by National High-tech Research and Development Projects (863) (2013AA10230402), National Natural Science Foundation of China (61473235), and the Major Pilot Projects of the Agro-Tech Extension and Service in Shaanxi (2016XXPT-05). NR 21 TC 0 Z9 0 U1 0 U2 0 PU CHINESE ACAD AGRICULTURAL ENGINEERING PI BEIJING PA RM 506, 41, MAIZIDIAN ST, CHAOYANG DISTRICT, BEIJING, 100125, PEOPLES R CHINA SN 1934-6344 EI 1934-6352 J9 INT J AGR BIOL ENG JI Int. J. Agric. Biol. Eng. PD NOV PY 2016 VL 9 IS 6 BP 148 EP 155 DI 10.3965/j.ijabe.20160906.2235 PG 8 WC Agricultural Engineering SC Agriculture GA EG1UL UT WOS:000390817900017 ER PT J AU Guo, LY Dunlap, CA Rooney, AP Chen, GJ Du, ZJ AF Guo, Ling-Yun Dunlap, Christopher A. Rooney, Alejandro P. Chen, Guan-Jun Du, Zong-Jun TI Wenzhouxiangella sediminis sp nov., isolated from coastal sediment SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID 16S RIBOSOMAL-RNA; PERFORMANCE LIQUID-CHROMATOGRAPHY; BACTERIUM; GENES AB A novel Gram-stain-negative, non-spore-forming, non-motile, facultatively anaerobic, rod-shaped strain, designated XDB06(T), was isolated from coastal sediment of Xiaoshi Island, Weihai, China. Optimal growth occurred at 37 degrees C, pH 7.5 and with 4.0% (w/v) NaCl. Q-8 was the sole respiratory quinone. The major cellular fatty acids in strain XDB06(T) were iso-C15:0 and isoC(16:0). The polar lipids of strain XDB06(T) were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified glycolipids and four unidentified phospholipids. The genomic DNA G+C content of the strain was 65.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XDB06(T) clusters within the genus Wenzhouxiangella and is most closely related to Wenzhouxiangella marina MCCC 1K00261(T), with a 16S rRNA gene sequence similarity of 96.50 %. Based on data from the current polyphasic study, strain XDB06(T) represents a novel species of the genus Wenzhouxiangella, for which the name Wenzhouxiangella sediminis sp. nov. is proposed. The type strain is XDB06(T) (=KCTC 52041(T) = MCCC 1K02285(T)). C1 [Guo, Ling-Yun; Chen, Guan-Jun; Du, Zong-Jun] Shandong Univ Weihai, Coll Marine Sci, Weihai 264209, Peoples R China. [Dunlap, Christopher A.; Rooney, Alejandro P.] USDA ARS, Natl Ctr Agr Utilizat Res, Crop Bioprotect Res Unit, Peoria, IL 61604 USA. [Chen, Guan-Jun; Du, Zong-Jun] Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Shandong, Peoples R China. RP Du, ZJ (reprint author), Shandong Univ Weihai, Coll Marine Sci, Weihai 264209, Peoples R China.; Du, ZJ (reprint author), Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Shandong, Peoples R China. EM duzongjun@sdu.edu.cn FU National Natural Science Foundation of China [31370057, 31290231]; Shandong Provincial Second Group Projects on Resource Platforms for Marine Economic and Innovative Development Regions: Marine Microorganisms Preservation Platform [2150299]; 111 Project [B16030] FX This work was supported by the National Natural Science Foundation of China (31370057, 31290231), 2013 Shandong Provincial Second Group Projects on Resource Platforms for Marine Economic and Innovative Development Regions: Marine Microorganisms Preservation Platform (2150299) and the 111 Project (no. B16030). NR 15 TC 0 Z9 0 U1 3 U2 3 PU MICROBIOLOGY SOC PI LONDON PA CHARLES DARWIN HOUSE, 12 ROGER ST, LONDON WC1N 2JU, ERKS, ENGLAND SN 1466-5026 EI 1466-5034 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD NOV PY 2016 VL 66 BP 4575 EP 4579 DI 10.1099/ijsem.0.001393 PN 11 PG 5 WC Microbiology SC Microbiology GA EF9YE UT WOS:000390686600041 PM 27498848 ER PT J AU Su, CC Deng, WL Jan, FJ Chang, CJ Huang, H Shih, HT Chen, J AF Su, C. -C Deng, W. -L. Jan, F. -J. Chang, C. -J. Huang, H. Shih, H. -T. Chen, J. TI Xylella taiwanensis sp nov., causing pear leaf scorch disease SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID 16S RIBOSOMAL-RNA; FASTIDIOSA STRAINS; PIERCES-DISEASE; SPECIES DEFINITION; SEQUENCE-ANALYSIS; GENOME SEQUENCES; SPACER SEQUENCES; BACTERIUM; PATHOGENICITY; CULTIVATION AB A Gram-stain-negative, nutritionally fastidious bacterium (PLS229(T)) causing pear leaf scorch was identified in Taiwan and previously grouped into Xylella fastidiosa. Yet, significant variations between PLS229(T) and Xylella fastidiosa were noted. In this study, PLS229(T) was evaluated phenotypically and genotypically against representative strains of Xylella fastidiosa, including strains of the currently known subspecies of Xylella fastidiosa, Xylella fastidiosa subsp. multiplex and 'Xylella fastidiosa subsp. pauca'. Because of the difficulty of in vitro culture characterization, emphases were made to utilize the available whole-genome sequence information. The average nucleotide identity (ANI) values, an alternative for DNA-DNA hybridization relatedness, between PLS229(T) and Xylella fastidiosa were 83.4-83.9%, significantly lower than the bacterial species threshold of 95 %. In contrast, sequence similarity of 16S rRNA genes was greater than 98 %, higher than the 97% threshold to justify if two bacterial strains belong to different species. The uniqueness of PLS229(T) was also evident by observing only about 87% similarity in the sequence of the 16S-23S internal transcribed spacer (ITS) between PLS229(T) and strains of Xylella fastidiosa, discovering significant single nucleotide polymorphisms at 18 randomly selected housekeeping gene loci, observing a distinct fatty acid profile for PLS229(T) compared with Xylella fastidiosa, and PLS229(T) having different observable phenotypes, such as different susceptibility to antibiotics. A phylogenetic tree derived from 16S rRNA gene sequences showed a distinct PLS229(T) phyletic lineage positioning it between Xylella fastidiosa and members of the genus Xanthomonas. On the basis of these data, a novel species, Xylella taiwanensis sp. nov. is proposed. The type strain is PLS229(T) (=BCRC 80915(T) = JCM 31187(T)). C1 [Su, C. -C] Taiwan Agr Chem & Tox Subst Res Inst, Div Pesticide Applicat, Taichung 41358, Taiwan. [Deng, W. -L.; Jan, F. -J.; Chang, C. -J.] Natl Chung Hsing Univ, Dept Plant Pathol, Taichung 40227, Taiwan. [Chang, C. -J.] Univ Georgia, Dept Plant Pathol, Griffin, GA 30223 USA. [Huang, H.] Univ S Florida, Sch Informat, Tampa, FL 33620 USA. [Shih, H. -T.] Council Agr, Taiwan Agr Res Inst, Appl Zool Div, 189 Chung Cheng Rd, Taichung 413, Taiwan. [Chen, J.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Chen, J (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. EM jianchi.chen@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service project [5302-22000-010-00D]; Taiwan Bureau of Animal and Plant Health Inspection and Quarantine, Council of Agriculture [99AS-9.3.1-BQ-B2, 101AS-10.2.1-BQ-B4] FX We thank C. M. Chang, Z. Zheng, R. Huerta and Greg Phillips for technical support and C. Wallis for critical editing of this manuscript. We also thank S. Marcelletti for assisting in ANI calculation. This research project was supported by the United States Department of Agriculture, Agricultural Research Service project 5302-22000-010-00D and the Taiwan Bureau of Animal and Plant Health Inspection and Quarantine, Council of Agriculture projects 99AS-9.3.1-BQ-B2 and 101AS-10.2.1-BQ-B4. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 46 TC 1 Z9 1 U1 4 U2 4 PU MICROBIOLOGY SOC PI LONDON PA CHARLES DARWIN HOUSE, 12 ROGER ST, LONDON WC1N 2JU, ERKS, ENGLAND SN 1466-5026 EI 1466-5034 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD NOV PY 2016 VL 66 BP 4766 EP 4771 DI 10.1099/ijsem.0.001426 PN 11 PG 6 WC Microbiology SC Microbiology GA EF9YE UT WOS:000390686600071 PM 27530392 ER PT J AU Pacheco, JM Stenfeldt, C Rodriguez, LL Arzt, J AF Pacheco, J. M. Stenfeldt, C. Rodriguez, L. L. Arzt, J. TI Infection Dynamics of Foot-and-Mouth Disease Virus in Cattle Following Intranasopharyngeal Inoculation or Contact Exposure SO JOURNAL OF COMPARATIVE PATHOLOGY LA English DT Article DE animal models; cattle; foot-and-mouth disease virus; pathogenesis ID SIMULATED-NATURAL INOCULATION; PARTIAL PROTECTION; EARLY PATHOGENESIS; SEROTYPE O; RT-PCR; TRANSMISSION; VACCINATION; PIGS; RESPONSES; CYTOKINE AB For the purpose of developing an improved experimental model for studies of foot-and-mouth disease virus (FMDV) infection in cattle, three different experimental systems based on natural or simulated natural virus exposure were compared under standardized experimental conditions. Ante-mortem infection dynamics were characterized in cattle exposed to FMDV through a novel, simulated natural intranasopharyngeal (INP) inoculation system or through standardized and controlled systems of within- or between-species direct contact exposure (cattle-to-cattle or pig-to-cattle). All three systems were efficient in causing synchronous, generalized foot-and-mouth disease in cattle exposed to one of three different strains of FMDV representing serotypes O, A and Asial. There was more within-group variation in the timing of clinical infection following natural and simulated natural virus exposure systems when compared with the conventionally used system of needle inoculation (intraepithelial lingual inoculation). However, the three optimized exposure systems described herein have the advantage of closely simulating field conditions by utilizing natural routes of primary infection, thereby facilitating engagement of mucosal host defence mechanisms. Overall, it is concluded that INP inoculation and standardized systems of direct contact exposure provide effective alternatives to conventional (needle) inoculation systems for studies in which it is desirable to simulate the natural biology of FMDV infection. Published by Elsevier Ltd. C1 [Pacheco, J. M.; Stenfeldt, C.; Rodriguez, L. L.; Arzt, J.] ARS, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, USDA, Greenport, NY 11944 USA. [Stenfeldt, C.] Oak Ridge Inst Sci & Educ, PIADC Res Participat Program, Oak Ridge, TN USA. RP Arzt, J (reprint author), ARS, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, USDA, Greenport, NY 11944 USA. EM Jonathan.Arzt@ars.usda.gov OI Arzt, Jonathan/0000-0002-7517-7893 FU CRIS [1940-32000-057-00D]; Science and Technology Directorate of the US Department of Homeland Security [HSHQDC-11-X-00189]; PIADC Research Participation Program fellowships FX This work was funded in part by CRIS project 1940-32000-057-00D (USDA, Agricultural Research Service), as well as through an interagency agreement with the Science and Technology Directorate of the US Department of Homeland Security under Award Number HSHQDC-11-X-00189. CS was recipient of PIADC Research Participation Program fellowships, administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement with the US Department of Energy. The sponsors had no involvement in the study design, in the collection, analysis and interpretation of data, in the writing of the manuscript, or in the decision to submit the manuscript for publication. The authors acknowledge and appreciate expert laboratory support provided by G. R. Smoliga, E. J. Hartwig, S. J. Pauszek and E. Bishop. The first two authors contributed equally to this work. NR 53 TC 0 Z9 0 U1 3 U2 3 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0021-9975 EI 1532-3129 J9 J COMP PATHOL JI J. Comp. Pathol. PD NOV PY 2016 VL 155 IS 4 BP 314 EP 325 DI 10.1016/j.jcpa.2016.08.005 PG 12 WC Pathology; Veterinary Sciences SC Pathology; Veterinary Sciences GA EF9AW UT WOS:000390624100006 PM 27697284 ER PT J AU Freeborn, PH Jolly, WM Cochrane, MA AF Freeborn, Patrick H. Jolly, W. Matt Cochrane, Mark A. TI Impacts of changing fire weather conditions on reconstructed trends in US wildland fire activity from 1979 to 2014 SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article DE daily fire associations; climate-fire relationships; magnitude of fire activity; natural hazards; climate impact; remote sensing; land; atmosphere interactions ID ROCKY-MOUNTAIN FORESTS; UNITED-STATES; RADIATIVE ENERGY; CLIMATE-CHANGE; FUEL CONSUMPTION; SIERRA-NEVADA; DETECTION ALGORITHM; SATELLITE SENSORS; PRESCRIBED FIRE; WESTERN USA AB One component of climate-fire interactions is the relationship between weather conditions concurrent with burning (i.e., fire danger) and the magnitude of fire activity. Here daily environmental conditions are associated with daily observations of fire activity within ecoregions across the continental United States (CONUS) by aligning the latter 12years of a 36year gridded fire danger climatology with the Moderate Resolution Imaging Spectroradiometer fire products. Results reveal that although modern relationships (2003-2014) vary regionally, fires across the majority of CONUS are more likely to be present and burning more vigorously as fire danger increases. Applying modern relationships to the entire climatology (1979-2014) indicates that in the absence of other influences, changes in fire danger have significantly increased the number of days per year that fires are burning across 42-49% of CONUS (by area) while also significantly increasing daily fire growth and daily heat release across 37-45% of CONUS. Increases in the fire activity season length coupled with an intensification of daily burning characteristics resulted in a CONUS-wide +0.02Mhayr(-1) trend in burned area, a 10.6gm(-2)yr(-1) trend in the amount of fuel consumed per unit burned area, and ultimately a +0.51Tgyr(-1) trend in dry matter consumption. Overall, the results demonstrate regional variations in the response of fires to changes in fire danger and that weather conditions concurrent with burning have a three-pronged impact on the magnitude of fire activity by affecting the seasonal duration, spatial extent, and combustion intensity. C1 [Freeborn, Patrick H.; Jolly, W. Matt] US Forest Serv, Rocky Mt Res Stn, Fire Sci Lab, Missoula, MT 59808 USA. [Cochrane, Mark A.] South Dakota State Univ, Geospatial Sci Ctr Excellence, Brookings, SD 57007 USA. RP Freeborn, PH (reprint author), US Forest Serv, Rocky Mt Res Stn, Fire Sci Lab, Missoula, MT 59808 USA. EM patrickhfreeborn@fs.fed.us FU NASA [NNH11ZDA001N-FIRES, NNX11AB89G, NNX12AQ88G] FX Funding is provided by NASA grants NNH11ZDA001N-FIRES, NNX11AB89G, and NNX12AQ88G. Thanks to those maintaining the University of Maryland ftp server (fuoco.geog.umd.edu) for hosting the MODIS direct broadcast (DB) burned area (MCD64A1) and global fire location (MCD14ML) products. Thanks also to the two reviewers and the Editor for their time and very constructive comments that have no doubt improved the final version of this manuscript. NR 92 TC 1 Z9 1 U1 3 U2 3 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD NOV PY 2016 VL 121 IS 11 BP 2856 EP 2876 DI 10.1002/2016JG003617 PG 21 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA EF9YD UT WOS:000390686500006 ER PT J AU Johnson, AC Yeakley, JA AF Johnson, Adelaide Chapman Yeakley, J. Alan TI Seedling Regeneration in the Alpine Treeline Ecotone: Comparison of Wood Microsites and Adjacent Soil Substrates SO MOUNTAIN RESEARCH AND DEVELOPMENT LA English DT Article DE Forest line; alpine treeline ecotone; plant water potential; moisture; temperature; conifer seedling regeneration; Pacific Northwest ID CONIFER SEEDLINGS; BRITISH-COLUMBIA; PINUS-CEMBRA; PICEA-ABIES; FORESTS; CLIMATE; TEMPERATURE; GROWTH; TIMBERLINE; SAPLINGS AB Although climate warming is generally expected to facilitate upward advance of forests, conifer seedling regeneration and survival may be hindered by low substrate moisture, high radiation, and both low and high snow accumulation. To better understand substrate-related factors promoting regeneration in the alpine treeline ecotone, this study compared 2 substrates supporting conifer seedlings: rotten downed wood and adjacent soil. Study locations, each with 3 levels of incoming radiation, were randomly selected at forest line-alpine meadow borders in Pacific Northwest wilderness areas extending along an east-west precipitation gradient. Associations among substrate type, seedling density, radiation, site moisture, site temperature, plant water potential, and plant stomatal conductance were assessed. Wood microsites, flush with the ground and supporting Abies spp conifer seedlings, extended up to 20 m into alpine meadows from the forest line. Although wood microsites thawed later in the spring and froze earlier in the fall, they had warmer summer temperatures, greater volumetric water content, and more growing degree hours, and seedlings growing on wood had higher water potentials than seedlings growing on adjacent soil. At drier eastern sites, there was a positive relationship between seedling density and volumetric water content. Further, there was a positive relationship between seedling stomatal conductance and volumetric water content. Our study indicates that in the Pacific Northwest. and likely elsewhere, seedlings benefit from wood microsites, which provide greater water content. Given predictions of increased summer drought in some locations globally, wood microsites at forest line-alpine meadows and forest line-grasslands borders may become increasingly important for successful conifer regeneration. C1 [Johnson, Adelaide Chapman] US Forest Serv, Pacific Northwest Res Stn, 11175 Auke Lake Way, Juneau, AK 99801 USA. [Yeakley, J. Alan] Univ Maryland Baltimore Cty, Dept Geog & Environm Syst, Baltimore, MD 21250 USA. RP Johnson, AC (reprint author), US Forest Serv, Pacific Northwest Res Stn, 11175 Auke Lake Way, Juneau, AK 99801 USA. EM ajohnson03@fs.fed.us NR 48 TC 0 Z9 0 U1 5 U2 5 PU MOUNTAIN RESEARCH & DEVELOPMENT PI LAWRENCE PA BUSINESS OFFICE, 810 E 10TH ST, PO BOX 1897, LAWRENCE, KANSAS 66044-8897 USA SN 0276-4741 EI 1994-7151 J9 MT RES DEV JI Mt. Res. Dev. PD NOV PY 2016 VL 36 IS 4 SI SI BP 443 EP 451 DI 10.1659/MRD-JOURNAL-D-16-00024R.1 PG 9 WC Environmental Sciences; Geography, Physical SC Environmental Sciences & Ecology; Physical Geography GA EF9UI UT WOS:000390676000005 ER PT J AU Hirsch, CN Hirsch, CD Brohammer, AB Bowman, MJ Soifer, I Barad, O Shem-Tov, D Baruch, K Lu, F Hernandez, AG Fields, CJ Wright, CL Koehler, K Springer, NM Buckler, E Buell, CR de Leon, N Kaeppler, SM Childs, KL Mikel, MA AF Hirsch, Candice N. Hirsch, Cory D. Brohammer, Alex B. Bowman, Megan J. Soifer, Ilya Barad, Omer Shem-Tov, Doron Baruch, Kobi Lu, Fei Hernandez, Alvaro G. Fields, Christopher J. Wright, Chris L. Koehler, Klaus Springer, Nathan M. Buckler, Edward Buell, C. Robin de Leon, Natalia Kaeppler, Shawn M. Childs, Kevin L. Mikel, Mark A. TI Draft Assembly of Elite Inbred Line PH207 Provides Insights into Genomic and Transcriptome Diversity in Maize SO PLANT CELL LA English DT Article ID COPY NUMBER VARIATION; ARABIDOPSIS-THALIANA; EUKARYOTIC GENOMES; QUALITY-CONTROL; READ ALIGNMENT; PLANT GENOMICS; OPEN SOFTWARE; ORYZA-SATIVA; PAN-GENOME; MAKER-P AB Intense artificial selection over the last 100 years has produced elite maize ( Zea mays) inbred lines that combine to produce high-yielding hybrids. To further our understanding of how genome and transcriptome variation contribute to the production of high-yielding hybrids, we generated a draft genome assembly of the inbred line PH207 to complement and compare with the existing B73 reference sequence. B73 is a founder of the Stiff Stalk germplasm pool, while PH207 is a founder of iodent germplasm, both of which have contributed substantially to the production of temperate commercial maize and are combined to make heterotic hybrids. Comparison of these two assemblies revealed over 2500 genes present in only one of the two genotypes and 136 gene families that have undergone extensive expansion or contraction. Transcriptome profiling revealed extensive expression variation, with as many as 10,564 differentially expressed transcripts and 7128 transcripts expressed in only one of the two genotypes in a single tissue. Genotype-specific genes were more likely to have tissue/condition-specific expression and lower transcript abundance. The availability of a high-quality genome assembly for the elite maize inbred PH207 expands our knowledge of the breadth of natural genome and transcriptome variation in elite maize inbred lines across heterotic pools. C1 [Hirsch, Candice N.; Brohammer, Alex B.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Hirsch, Cory D.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Bowman, Megan J.; Buell, C. Robin; Childs, Kevin L.] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. [Soifer, Ilya] Calico Labs, San Francisco, CA 94080 USA. [Barad, Omer; Shem-Tov, Doron; Baruch, Kobi] NRGENE Ltd, IL-7403648 Ness Ziona, Israel. [Lu, Fei; Buckler, Edward] Cornell Univ, Inst Genome Divers, Ithaca, NY 14850 USA. [Hernandez, Alvaro G.; Fields, Christopher J.; Wright, Chris L.; Mikel, Mark A.] Univ Illinois, Roy J Carver Biotechnol Ctr, Urbana, IL 61801 USA. [Koehler, Klaus] Dow AgroSci, Indianapolis, IN 46268 USA. [Springer, Nathan M.] Univ Minnesota, Dept Plant Biol, St Paul, MN 55108 USA. [Buckler, Edward] ARS, USDA, Ithaca, NY 14850 USA. [Buell, C. Robin] DOE Great Lakes Bioenergy Res Ctr, E Lansing, MI 48824 USA. [de Leon, Natalia; Kaeppler, Shawn M.] Univ Wisconsin, Dept Agron, 1575 Linden Dr, Madison, WI 53706 USA. [de Leon, Natalia; Kaeppler, Shawn M.] DOE Great Lakes Bioenergy Res Ctr, Madison, WI 53706 USA. [Childs, Kevin L.] Michigan State Univ, Ctr Genom Enabled Plant Sci, E Lansing, MI 48824 USA. [Mikel, Mark A.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. RP Hirsch, CN (reprint author), Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. EM cnhirsch@umn.edu OI Bowman, Megan/0000-0001-5742-1779; Hirsch, Cory/0000-0002-3409-758X FU DOE Great Lakes Bioenergy Research Center (DOE BER Office of Science) [DE-FC02-07ER64494]; Dow AgroSciences; National Science Foundation [IOS-1126998]; National Science Foundation National Plant Genome Initiative Postdoctoral Fellowship in Biology Fellowship [1202724]; DuPont Pioneer Bill Kuhn Honorary Fellowship FX This work was funded in part by the DOE Great Lakes Bioenergy Research Center (DOE BER Office of Science DE-FC02-07ER64494), by Dow AgroSciences, and by the National Science Foundation (Grant IOS-1126998 to K.L.C.). The Minnesota Supercomputing Institute at the University of Minnesota provided computational resources that contributed to the research results reported in this article. C.D.H. was supported by a National Science Foundation National Plant Genome Initiative Postdoctoral Fellowship in Biology Fellowship (Grant 1202724). A.B.B. was supported by the DuPont Pioneer Bill Kuhn Honorary Fellowship. NR 89 TC 0 Z9 0 U1 3 U2 3 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD NOV PY 2016 VL 28 IS 11 BP 2700 EP 2714 DI 10.1105/tpc.16.00353 PG 15 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA EG1NR UT WOS:000390800000003 PM 27803309 ER PT J AU Guard, J Rothrock, MJ Shah, DH Jones, DR Gast, RK Sanchez-Ingunza, R Madsen, M El-Attrache, J Lungu, B AF Guard, Jean Rothrock, Michael J. Shah, Devendra H. Jones, Deana R. Gast, Richard K. Sanchez-Ingunza, Roxana Madsen, Melissa El-Attrache, John Lungu, Bwalya TI Metabolic parameters linked by phenotype microarray to acid resistance profiles of poultry-associated Salmonella enterica SO RESEARCH IN MICROBIOLOGY LA English DT Article DE Salmonella; Food safety; Phenotype; Acid tolerance; Metabolism; Poultry ID TOLERANCE RESPONSE; SEROVAR TYPHIMURIUM; ENTERITIDIS; EXPRESSION; VIRULENCE; STRESS; HENS; SEFD AB Phenotype microarrays were analyzed for 51 datasets derived from Salmonella enterica. The top 4 serotypes associated with poultry products and one associated with turkey, respectively Typhimurium, Enteritidis, Heidelberg, Infantis and Senftenberg, were represented. Datasets were partitioned initially into two clusters based on ranking by values at pH 4.5 (PM10 A03). Negative control wells were used to establish 90 respiratory units as the point differentiating acid resistance from sensitive strains. Thus, 24 isolates that appeared most acid-resistant were compared initially to 27 that appeared most acid-sensitive (24 x 27 format). Paired cluster analysis was also done and it included the 7 most acid-resistant and -sensitive datasets (7 x 7 format). Statistical analyses of ranked data were then calculated in order of standard deviation, probability value by the Student's t-test and a measure of the magnitude of difference called effect size. Data were reported as significant if, by order of filtering, the following parameters were calculated: i) a standard deviation of 24 respiratory units or greater from all datasets for each chemical, ii) a probability value of less than or equal to 0.03 between clusters and iii) an effect size of at least 0.50 or greater between clusters. Results suggest that between 7.89% and 23.16% of 950 chemicals differentiated acid-resistant isolates from sensitive ones, depending on the format applied. Differences were more evident at the extremes of phenotype using the subset of data in the paired 7 x 7 format. Results thus provide a strategy for selecting compounds for additional research, which may impede the emergence of acid-resistant Salmonella enterica in food. Published by Elsevier Masson SAS on behalf of Institut Pasteur. C1 [Guard, Jean; Rothrock, Michael J.; Jones, Deana R.; Gast, Richard K.] USDA, US Natl Poultry Res Lab, 950 Coll Stn Rd, Athens, GA 30605 USA. [Shah, Devendra H.] Washington State Univ, Pullman, WA 99164 USA. [Sanchez-Ingunza, Roxana; Madsen, Melissa; El-Attrache, John] CEVA, Lenexa, KS USA. [Lungu, Bwalya] Univ Calif Davis, Davis, CA 95616 USA. RP Guard, J (reprint author), USDA, US Natl Poultry Res Lab, 950 Coll Stn Rd, Athens, GA 30605 USA. EM Jean.guard@ars.usda.gov; Michael.rothrock@ars.usda.gov; dshah@vetmed.wsu.edu; deana.jones@ars.usda.gov; Richard.gast@ars.usda.gov; Roxana.sanchez@ceva.com; Melissa.madsen@ceva.com; john.elattrache@ceva.com; blungu@ucdavis.edu OI Shah, Devendra/0000-0001-6365-7868 FU ARS [6040-32000-007-D, 6040-32420-001-00] FX This research was primarily supported by ARS Project Number 6040-32000-007-D and supplementary support from ARS Project Number 6040-32420-001-00. CEVA and Aviagen (R) provided some travel support and collaboration under material transfer agreements, which protected rights to authorship by the USDA. Bwalya Lungu was an employee of Aviagen while participating in this research. Jean Guard, with assistance from laboratory technician Tod Stewart, conducted phenotype microarray. Collaborators contributed bacteria and essential support exceeding 3 years. We also want to thank Michael McClelland and colleagues for their contributions of isolates and continued support on genomic analyses indirectly related to this manuscript. The complete dataset for PM plates 1-10 is available upon request in spreadsheet form according to USDA guidelines for a material transfer research agreement (MTRA). NR 23 TC 1 Z9 1 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0923-2508 EI 1769-7123 J9 RES MICROBIOL JI Res. Microbiol. PD NOV-DEC PY 2016 VL 167 IS 9-10 SI SI BP 745 EP 756 DI 10.1016/j.resmic.2016.06.006 PG 12 WC Microbiology SC Microbiology GA EF9AZ UT WOS:000390624400005 PM 27418207 ER PT J AU Warren, SD Baggett, LS Warren, H AF Warren, Steven D. Baggett, L. Scott Warren, Heather TI DIRECTIONAL FLORAL ORIENTATION IN JOSHUA TREES (YUCCA BREVIFOLIA) SO WESTERN NORTH AMERICAN NATURALIST LA English DT Article ID FLOWER ORIENTATION; CONSEQUENCES; POLLINATORS; AGAVACEAE; CACTACEAE; REWARD; DESERT; MOTHS; HEAT AB Joshua tree (Yucca brevifolia Engelm.) is a large, arborescent member of the yucca genus. It is an endemic and visually dominant plant in portions of the Mojave Desert, USA. We document the unique and heretofore unreported directional orientation of its flower panicles. The flower panicles grow primarily at the tips of branches that are oriented to the south. When branches with flower panicles are not oriented in a southerly direction, the flower panicles themselves tend to bend or tilt toward the south. This strategy maximizes exposure of the panicles to direct solar radiation, which, within the latitudes where the Joshua tree grows, is always from the south. Such a strategy may minimize the energetic cost of translocating photosynthates from the plant's leaf rosettes to the flowers. The flower panicles create large, light-colored landing pads for the obligate nocturnal moth pollinator. Residual warmth in the flower panicles may provide a thermal reward for the moth pollinator that emerges shortly after sunset. C1 [Warren, Steven D.] US Forest Serv, USDA, Rocky Mt Res Stn, Provo, UT 84606 USA. [Baggett, L. Scott] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO USA. RP Warren, SD (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Provo, UT 84606 USA. EM swarren02@fs.fed.us NR 31 TC 0 Z9 0 U1 6 U2 6 PU BRIGHAM YOUNG UNIV PI PROVO PA 290 LIFE SCIENCE MUSEUM, PROVO, UT 84602 USA SN 1527-0904 EI 1944-8341 J9 WEST N AM NATURALIST JI West. North Am. Naturalist PD NOV PY 2016 VL 76 IS 3 BP 374 EP 378 PG 5 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA EF8QJ UT WOS:000390593700012 ER PT J AU Fowler, JF Overby, S AF Fowler, James F. Overby, Steven TI SNOW DURATION EFFECTS ON DENSITY OF THE ALPINE ENDEMIC PLANT PACKERA FRANCISCANA SO WESTERN NORTH AMERICAN NATURALIST LA English DT Article ID ASTERACEAE; PARAMO AB Packera franciscana (Greene) W.A. Weber and A. Love (Asteraceae) (San Francisco Peaks ragwort) is an alpine-zone endemic of the San Francisco Peaks in northern Arizona. Previous studies have shown that P. franciscana is patchily distributed in alpine-zone talus habitats. The purpose of this study was to describe the relationship between snow duration and P. franciscana abundance. We established trailside transects through P. franciscana habitat along the Weatherford Trail to estimate the abundance of P. franciscana ramets. Snow-free and snowbed sample segments were chosen based on a 17 May 2013 snow photograph taken from within the Inner Basin of the San Francisco Peaks caldera. The section of the Weatherford Trail under snow on 17 May 2013 had a 5-year arithmetic mean of 1.53 ramets . m(-2) (SE 0.37)-much less dense than the snow-free section which had a 5-year arithmetic mean of 7.17 ramets . m(-2) (SE 1.09). The steep upper portion of talus slopes is known to move downslope faster than mid-and lower slope portions due to gravitational processes working on surface particle size, slope angle, and frost heave/needle ice during moist periods. Needle ice is known to uproot seedlings in other species. Active talus shift may inhibit P. franciscana colonization and growth on upper talus slopes via high seedling mortality in the snowbed zone during spring snowmelt. C1 [Fowler, James F.; Overby, Steven] US Forest Serv, USDA, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. RP Fowler, JF (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. EM alpineworkshop@gmail.com FU Coconino National Forest; Rocky Mountain Research Station (RMRS), Forest and Woodland Ecosystems Science Program FX Financial support for this research was provided by the Coconino National Forest and the Rocky Mountain Research Station (RMRS), Forest and Woodland Ecosystems Science Program. Ben Bright prepared Fig. 1. We thank our field crew members for dedication to this work: Brian Casavant, Addie Hite, Scott Vojta, and Suzanne Owen. Voucher specimens were collected under U.S. Fish and Wildlife permit TE225821-0 and are deposited in the RMRS Flagstaff Herbarium (ASUF). NR 23 TC 0 Z9 0 U1 0 U2 0 PU BRIGHAM YOUNG UNIV PI PROVO PA 290 LIFE SCIENCE MUSEUM, PROVO, UT 84602 USA SN 1527-0904 EI 1944-8341 J9 WEST N AM NATURALIST JI West. North Am. Naturalist PD NOV PY 2016 VL 76 IS 3 BP 383 EP 387 PG 5 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA EF8QJ UT WOS:000390593700014 ER PT J AU Field, JL Marx, E Easter, M Adler, PR Paustian, K AF Field, John L. Marx, Ernie Easter, Mark Adler, Paul R. Paustian, Keith TI Ecosystem model parameterization and adaptation for sustainable cellulosic biofuel landscape design SO GLOBAL CHANGE BIOLOGY BIOENERGY LA English DT Article DE biogeochemistry; carbon sequestration; cellulosic biofuel; ecosystem model parameterization; ecosystem modeling; feedstock; greenhouse gas emissions; landscape design; marginal land; nitrous oxide ID MISCANTHUS X GIGANTEUS; LAND-USE-CHANGE; SWITCHGRASS BIOMASS PRODUCTION; BIOENERGY CROP PRODUCTIVITY; GREENHOUSE-GAS EMISSIONS; WATER-USE EFFICIENCY; US GREAT-PLAINS; PANICUM-VIRGATUM; UNITED-STATES; NITROGEN-FERTILIZATION AB Renewable fuel standards in the US and elsewhere mandate the production of large quantities of cellulosic biofuels with low greenhouse gas (GHG) footprints, a requirement which will likely entail extensive cultivation of dedicated bioenergy feedstock crops on marginal agricultural lands. Performance data for such systems is sparse, and non-linear interactions between the feedstock species, agronomic management intensity, and underlying soil and land characteristics complicate the development of sustainable landscape design strategies for low-impact commercial-scale feedstock production. Process-based ecosystem models are valuable for extrapolating field trial results and making predictions of productivity and associated environmental impacts that integrate the effects of spatially variable environmental factors across diverse production landscapes. However, there are few examples of ecosystem model parameterization against field trials on both prime and marginal lands or of conducting landscape-scale analyses at sufficient resolution to capture interactions between soil type, land use, and management intensity. In this work we used a data-diverse, multi-criteria approach to parameterize and validate the DayCent biogeochemistry model for upland and lowland switchgrass using data on yields, soil carbon changes, and soil nitrous oxide emissions from US field trials spanning a range of climates, soil types, and management conditions. We then conducted a high-resolution case study analysis of a real-world cellulosic biofuel landscape in Kansas in order to estimate feedstock production potential and associated direct biogenic GHG emissions footprint. Our results suggest that switchgrass yields and emissions balance can vary greatly across a landscape large enough to supply a biorefinery in response to variations in soil type and land-use history, but that within a given land base both of these performance factors can be widely modulated by changing management intensity. This in turn implies a large sustainable cellulosic biofuel landscape design space within which a system can be optimized to meet economic or environmental objectives. C1 [Field, John L.; Marx, Ernie; Easter, Mark; Paustian, Keith] Colorado State Univ, Nat Resource Ecol Lab, 1499 Campus Delivery, Ft Collins, CO 80523 USA. [Field, John L.] Colorado State Univ, Dept Mech Engn, 1374 Campus Delivery, Ft Collins, CO 80523 USA. [Adler, Paul R.] ARS, Pasture Syst & Watershed Management Res Unit, USDA, University Pk, PA 16802 USA. [Paustian, Keith] Colorado State Univ, Dept Soil & Crop Sci, 1170 Campus Delivery, Ft Collins, CO 80523 USA. RP Field, JL (reprint author), Colorado State Univ, Nat Resource Ecol Lab, 1499 Campus Delivery, Ft Collins, CO 80523 USA.; Field, JL (reprint author), Colorado State Univ, Dept Mech Engn, 1374 Campus Delivery, Ft Collins, CO 80523 USA. EM John.L.Field@colostate.edu FU USDA/NIFA project [2011-67009-30083]; NSF IGERT fellowship through the Multidisciplinary Approaches to Sustainable Bioenergy program at Colorado State University; NSF REU fellowship; graduate Chevron fellowship through the Colorado Center for Biorefining and Biofuels (C2B2) FX This work was supported by a USDA/NIFA project 'Decision support tool for integrated biofuel greenhouse gas emission footprints' (grant # 2011-67009-30083), an NSF IGERT fellowship through the Multidisciplinary Approaches to Sustainable Bioenergy program at Colorado State University, and an NSF REU fellowship and graduate Chevron fellowship through the Colorado Center for Biorefining and Biofuels (C2B2). We would like to thank Jacqueline Marquez for her assistance in identifying and coding switchgrass field trial papers, Matthew Stermer for helping quality-controlling this dataset, Kendrick Killian and Ty Boyak for their advice on developing model automation and analysis code, Jill Schuler for her GIS contribution, Jeff Kent for help with DayCent growth submodel performance visualization, and Yao Zhang for his insights on crop model performance in dry climates. NR 135 TC 0 Z9 0 U1 5 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1757-1693 EI 1757-1707 J9 GCB BIOENERGY JI GCB Bioenergy PD NOV PY 2016 VL 8 IS 6 BP 1106 EP 1123 DI 10.1111/gcbb.12316 PG 18 WC Agronomy; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA EF0TL UT WOS:000390038800007 ER PT J AU Stewart, CE Follett, RF Pruessner, EG Varvel, GE Vogel, KP Mitchell, RB AF Stewart, Catherine E. Follett, Ronald F. Pruessner, Elizabeth G. Varvel, Gary E. Vogel, Kenneth P. Mitchell, Robert B. TI N fertilizer and harvest impacts on bioenergy crop contributions to SOC SO GLOBAL CHANGE BIOLOGY BIOENERGY LA English DT Article DE harvest timing; N fertilizer; residue removal; roots; soil C sequestration; soil fractions; soil organic C ID CORN RESIDUE REMOVAL; SOIL ORGANIC-MATTER; LITTER DECOMPOSITION; CARBON SEQUESTRATION; SWITCHGRASS; NITROGEN; BIOMASS; ROOTS; MANAGEMENT; PRODUCTIVITY AB Belowground root biomass is infrequently measured and simply represented in models that predict landscape-level changes to soil carbon stocks and greenhouse gas balances. Yet, crop-specific responses to N fertilizer and harvest treatments are known to impact both plant allocation and tissue chemistry, potentially altering decomposition rates and the direction and magnitude of soil C stock changes and greenhouse gas fluxes. We examined switchgrass (Panicum virgatum L.) and corn (Zea mays L.,) yields, belowground root biomass, C, N and soil particulate organic matter-C (POM-C) in a 9-year rainfed study of N fertilizer rate (0, 60, 120 and 180 kg N ha(-1)) and harvest management near Mead, NE, USA. Switchgrass was harvested with one pass in either August or postfrost, and for no-till (NT) corn, either 50% or no stover was removed. Switchgrass had greater belowground root biomass C and N (6.39, 0.10 Mg ha(-1)) throughout the soil profile compared to NT-corn (1.30, 0.06 Mg ha(-1)) and a higher belowground root biomass C: N ratio, indicating greater recalcitrant belowground root biomass C input beneath switchgrass. There was little difference between the two crops in soil POM-C indicating substantially slower decomposition and incorporation into SOC under switchgrass, despite much greater root C. The highest N rate decreased POM-C under both NT-corn and switchgrass, indicating faster decomposition rates with added fertilizer. Residue removal reduced corn belowground root biomass C by 37% and N by 48% and subsequently reduced POM-C by 22% compared to no-residue removal. Developing productive bioenergy systems that also conserve the soil resource will require balancing fertilization that maximizes aboveground productivity but potentially reduces SOC sequestration by reducing belowground root biomass and increasing root and soil C decomposition. C1 [Stewart, Catherine E.; Follett, Ronald F.; Pruessner, Elizabeth G.] ARS, Soil Plant Nutrient Res Unit, USDA, Suite 100,2150 Ctr Ave,Bldg D, Ft Collins, CO 80526 USA. [Varvel, Gary E.] Univ Nebraska, Agroecosyst Management Res Unit, USDA, ARS, 251 Filley Hall Food Ind Complex, Lincoln, NE 68583 USA. [Vogel, Kenneth P.; Mitchell, Robert B.] Univ Nebraska, Grain Forage & Bioenergy Res Unit, USDA, ARS, 251 Filley Hall Food Ind Complex, Lincoln, NE 68683 USA. RP Stewart, CE (reprint author), ARS, Soil Plant Nutrient Res Unit, USDA, Suite 100,2150 Ctr Ave,Bldg D, Ft Collins, CO 80526 USA. EM catherine.stewart@colostate.edu OI Stewart, Catherine/0000-0003-1216-0450 FU U.S. Department of Agriculture (USDA), Agricultural Research Service (ARS); USDA Natural Resources Conservation Service FX This research was funded by the U.S. Department of Agriculture (USDA), Agricultural Research Service (ARS) and the USDA Natural Resources Conservation Service. Authors acknowledge the important technical contributions of Edward Buenger, Susan Sampson-Liebig, Steven Masterson, Patrick Callahan, Kevin Grams, Stephan Swanson and many student employees. The field assistance and coordination by Steve Scheinost, Asst. State Soil Scientist, USDA, NRCS, for Nebraska and laboratory support provided by the USDA, NRCS, Soil Survey Laboratory (National Soil Survey Center) are acknowledged. This work is part of the USDA-ARS GRACEnet network. NR 40 TC 0 Z9 0 U1 5 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1757-1693 EI 1757-1707 J9 GCB BIOENERGY JI GCB Bioenergy PD NOV PY 2016 VL 8 IS 6 BP 1201 EP 1211 DI 10.1111/gcbb.12326 PG 11 WC Agronomy; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA EF0TL UT WOS:000390038800014 ER PT J AU Graves, F Baker, TC Zhang, AJ Keena, M Hoover, K AF Graves, Fern Baker, Thomas C. Zhang, Aijun Keena, Melody Hoover, Kelli TI Sensory Aspects of Trail-Following Behaviors in the Asian Longhorned Beetle, Anoplophora glabripennis SO JOURNAL OF INSECT BEHAVIOR LA English DT Article DE Anoplophora glabripennis; trail following; sex pheromones; gustatory detection; mate-finding behavior ID COLEOPTERA CERAMBYCIDAE; SEX-PHEROMONE; UNITED-STATES; CUES; MALES; ANT AB Anoplophora glabripennis has a complex suite of mate-finding behaviors, the functions of which are not entirely understood. These behaviors are elicited by a number of factors, including visual and chemical cues. Chemical cues include a male-produced volatile semiochemical acting as a long-range sex pheromone, a female-produced cuticular hydrocarbon blend serving as a sex-identification contact pheromone, and a recently identified female-produced trail sex pheromone that is followed by mate-seeking males. However, the sensory appendages and sensilla on these appendages used to detect the trail sex pheromone are unknown. We evaluated the ability of virgin male A. glabripennis to follow a sex pheromone trail after removal of the terminal four antennal segments and/or the maxillary and labial palps using a two-choice behavioral bioassay. We also tested the ability of males to follow the trail sex pheromone using volatile pheromone cues only, without physical contact with the pheromone. Results indicate that the palps are primarily responsible for sensing the pheromone, with males lacking palps unable to respond behaviorally to the trail sex pheromone. Under the conditions of this study, males could not follow the sex pheromone trail without direct contact, suggesting that olfaction may not be involved in detection of this pheromone. However, we did not determine to what degree the trail pheromone chemicals can volatilize under our experimental conditions. This work is important in elucidating the behaviors and sensory structures involved in mate-finding by this species on host trees, and these studies may help determine whether the trail sex pheromone has applications for monitoring and management. C1 [Graves, Fern; Baker, Thomas C.; Hoover, Kelli] Penn State Univ, Dept Entomol, 501 ASI Bldg, University Pk, PA 16802 USA. [Zhang, Aijun] ARS, USDA, Invas Insect Biocontrol & Behav Lab, BARC West, Beltsville, MD 20705 USA. [Keena, Melody] US Forest Serv, USDA, Northern Res Stn, 51 Mill Pond Rd, Hamden, CT 06514 USA. RP Hoover, K (reprint author), Penn State Univ, Dept Entomol, 501 ASI Bldg, University Pk, PA 16802 USA. EM kxh25@psu.edu FU Alphawood Foundation; US Department of Agriculture, National Institute of Food and Agriculture Grant [2015-67013-23287] FX Funding for this work was provided by Alphawood Foundation and US Department of Agriculture, National Institute of Food and Agriculture Grant 2015-67013-23287 to KH. NR 33 TC 1 Z9 1 U1 7 U2 7 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0892-7553 EI 1572-8889 J9 J INSECT BEHAV JI J. Insect Behav. PD NOV PY 2016 VL 29 IS 6 BP 615 EP 628 DI 10.1007/s10905-016-9587-8 PG 14 WC Entomology SC Entomology GA EF3WD UT WOS:000390254700002 ER PT J AU Tomsa, A Bartz, SK Krishnamurthy, R Krishnamurthy, R Bacha, F AF Tomsa, Anca Bartz, Sara Klinepeter Krishnamurthy, Rajesh Krishnamurthy, Ramkumar Bacha, Fida TI Endothelial Function in Youth: A Biomarker Modulated by Adiposity-Related Insulin Resistance SO JOURNAL OF PEDIATRICS LA English DT Article ID PERIPHERAL ARTERIAL TONOMETRY; CARDIOVASCULAR RISK-FACTORS; TYPE-2 DIABETES-MELLITUS; DIGITAL REACTIVE HYPEREMIA; VASCULAR FUNCTION; HEALTHY-CHILDREN; OBESE CHILDREN; HEART-DISEASE; ADOLESCENTS; DYSFUNCTION AB Objective To investigate the physical and metabolic determinants of endothelial dysfunction, an early marker of subclinical atherosclerosis, in normal weight and overweight adolescents with and without type 2 diabetes mellitus. Study design A cross-sectional study of 81 adolescents: 21 normal weight, 25 overweight with normal glucose tolerance, 19 overweight with impaired glucose regulation, and 16 with type 2 diabetes mellitus underwent evaluation of reactive hyperemia index (RHI) and augmentation index (AIx) at heart rate 75 bpm by peripheral arterial tonometry; oral glucose tolerance test, lipid profile, and hyperinsulinemic-euglycemic clamp to measure insulin sensitivity; and dual energy X-ray absorptiometry scan and abdominal magnetic resonance imaging for percentage of body fat and abdominal fat partitioning. Results Participants across tertiles of RHI (1.2 +/- 0.02, 1.5 +/- 0.02, and 2.0 +/- 0.05, P < .001) had similar age, sex, race, lipid profile, and blood pressure. Body mass index z-score, percentage body fat, abdominal fat, and hemoglobin A1c decreased, and insulin sensitivity increased from the first to third tertile. RHI was inversely related to percentage body fat (r = -0.29, P = .008), total (r = -0.37, P = .004), subcutaneous (r = -0.39, P = .003), and visceral (r = -0.26, P = .04) abdominal fat. AIx at heart rate 75 bpm was higher (worse) in the lower RHI tertiles (P = .04), was positively related to percentage body fat (r = 0.26, P = .021), and inversely related to age, insulin sensitivity, and inflammatory markers (tumor necrosis factor-a and plasminogen activator inhibition-1). Conclusions Childhood obesity, particularly abdominal adiposity, is associated with endothelial dysfunction manifested by worse reactive hyperemia and higher AIx. Insulin resistance appears to mediate this relationship. C1 [Tomsa, Anca; Bartz, Sara Klinepeter; Bacha, Fida] Texas Childrens Hosp, Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Tomsa, Anca; Bartz, Sara Klinepeter; Bacha, Fida] Texas Childrens Hosp, Baylor Coll Med, Div Pediat Endocrinol & Diabet, Houston, TX 77030 USA. [Krishnamurthy, Rajesh; Krishnamurthy, Ramkumar] Texas Childrens Hosp, Baylor Coll Med, Dept Radiol, Houston, TX 77030 USA. RP Bacha, F (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM fbacha@bcm.edu FU US Department of Agriculture-Agricultural Research Service Award [6250-51000-057]; Thrasher Research Fund FX Supported by the US Department of Agriculture-Agricultural Research Service Award (6250-51000-057) and Thrasher Research Fund. The authors declare no conflicts to disclose. NR 36 TC 0 Z9 0 U1 1 U2 1 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-3476 EI 1097-6833 J9 J PEDIATR-US JI J. Pediatr. PD NOV PY 2016 VL 178 BP 171 EP 177 DI 10.1016/j.jpeds.2016.07.025 PG 7 WC Pediatrics SC Pediatrics GA EF0ON UT WOS:000390025400032 PM 27546204 ER PT J AU Duncan, NP Kahl, SS Gray, SS Salice, CJ Stevens, RD AF Duncan, Nathan P. Kahl, Samantha S. Gray, Shawn S. Salice, Christopher J. Stevens, Richard D. TI Pronghorn Habitat Suitability in the Texas Panhandle SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE Antilocapra americana; expert opinion; habitat suitability; MAXENT; pronghorn; Texas Panhandle ID SPECIES DISTRIBUTIONS; ANTELOPE; QUALITY; UTILITY; FORAGE; MAXENT AB Habitat quality is an important factor that can greatly affect wildlife populations. Pronghorn (Antilocapra americana) habitat in the Texas Panhandle, USA has been lost through growth of human settlements and agricultural lands. We determined the most pertinent environmental variables affecting habitat selection using multiple methods, including a search of peer-reviewed literature, expert opinion ranking, and habitat suitability modeling. We determined quality and extent of pronghorn habitat in the Texas Panhandle using the MAXENT modeling environment to build a presence-only habitat suitability model based on global positioning system (GPS) locations collected via aerial surveys. Our habitat suitability model indicated that woodlands, agricultural land, and summer precipitation had the greatest contributions to the overall model. Areas with greatest habitat suitability are associated with high pronghorn population densities, particularly in the northwestern corner of the Panhandle. This probabilistic model may serve as a useful tool for pronghorn conservation primarily because it provides insight into what factors are most predictive of their presence, which areas aremost suitable for pronghorn, and as a simple, replicable process to identify and evaluate pronghorn habitat. (C) 2016 The Wildlife Society. C1 [Duncan, Nathan P.] US Forest Serv, Gila Natl Forest, POB 170, Reserve, NM 87830 USA. [Kahl, Samantha S.; Stevens, Richard D.] Texas Tech Univ, Dept Nat Resources Management, MS 42125, Lubbock, TX 79409 USA. [Gray, Shawn S.] Texas Parks & Wildlife Dept, 109 S Cockrell, Alpine, TX 79830 USA. [Salice, Christopher J.] Towson Univ, Environm Sci & Studies Program, 8000 York Rd, Towson, MD 21252 USA. RP Duncan, NP (reprint author), US Forest Serv, Gila Natl Forest, POB 170, Reserve, NM 87830 USA. EM nathanduncan@fs.fed.us FU Texas Tech University FX We thank the TPWD for the data they provided for this study and Texas Tech University for providing funding. NR 61 TC 0 Z9 0 U1 7 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD NOV PY 2016 VL 80 IS 8 BP 1471 EP 1478 DI 10.1002/jwmg.21139 PG 8 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA EF0UC UT WOS:000390040600013 ER PT J AU Krishnan, HB Alaswad, AA Oehrle, NW Gillman, JD AF Krishnan, Hari B. Alaswad, Alaa A. Oehrle, Nathan W. Gillman, Jason D. TI Deletion of the SACPD-C Locus Alters the Symbiotic Relationship Between Bradyrhizobium japonicum USDA110 and Soybean, Resulting in Elicitation of Plant Defense Response and Nodulation Defects SO MOLECULAR PLANT-MICROBE INTERACTIONS LA English DT Article ID CARRIER-PROTEIN-DESATURASE; STEARIC-ACID LEVELS; RHIZOBIA-LEGUME SYMBIOSIS; NITROGEN-FIXING NODULES; FATTY-ACID; SALICYLIC-ACID; JASMONIC-ACID; GENE-EXPRESSION; NUCLEOTIDE-SEQUENCE; SIGNALING PATHWAYS AB Legumes form symbiotic associations with soil-dwelling bacteria collectively called rhizobia. This association results in the formation of nodules, unique plant-derived organs, within which the rhizobia are housed. Rhizobia-encoded nitrogenase facilitates the conversion of atmospheric nitrogen into ammonia, which is utilized by the plants for its growth and development. Fatty acids have been shown to play an important role in root nodule symbiosis. In this study, we have investigated the role of stearoyl-acyl carrier protein desaturase isoform C (SACPD-C), a soybean enzyme that catalyzes the conversion of stearic acid into oleic acid, which is expressed in developing seeds and in nitrogen-fixing nodules. In-depth cytological investigation of nodule development in sacpd-c mutant lines M25 and MM106 revealed gross anatomical alteration in the sacpd-c mutants. Transmission electron microscopy observations revealed ultrastructural alterations in the sacpd-c mutants that are typically associated with plant defense response to pathogens. In nodules of two sacpd-c mutants, the combined jasmonic acid (JA) species (JA and the isoleucine conjugate of JA) were found to be reduced and 12-oxophytodienoic acid (OPDA) levels were significantly higher relative to wild-type lines. Salicylic acid levels were not significantly different between genotypes, which is divergent from previous studies of sacpd mutant studies on vegetative tissues. Soybean nodule phytohormone profiles were very divergent from those of roots, and root profiles were found to be almost identical between mutant and wild-type genotypes. The activities of antioxidant enzymes, ascorbate peroxidase, and superoxide dismutase were also found to be higher in nodules of sacpd-c mutants. PR-I gene expression was extremely elevated in M25 and MM106, while the expression of nitrogenase was significantly reduced in these sacpd-c mutants, compared with the parent 'Bay'. Two-dimensional gel electrophoresis and matrix-assisted laser desorption-ionization time of flight mass spectrometry analyses confirmed sacpd-c mutants also accumulated higher amounts of pathogenesis-related proteins in the nodules. Our study establishes a major role for SACPD-C activity as essential for proper maintenance of soybean nodule morphology and physiology and indicates that OPDA signaling is likely to be involved in attenuation of nodule biotic defense responses. C1 [Krishnan, Hari B.; Oehrle, Nathan W.; Gillman, Jason D.] USDA ARS, Plant Genet Res Unit, Columbia, MO 65211 USA. [Krishnan, Hari B.; Alaswad, Alaa A.; Gillman, Jason D.] Univ Missouri, Plant Sci Div, Columbia, MO 65211 USA. [Alaswad, Alaa A.] King Abdulaziz Univ, Jeddah, Saudi Arabia. RP Krishnan, HB (reprint author), USDA ARS, Plant Genet Res Unit, Columbia, MO 65211 USA.; Krishnan, HB (reprint author), Univ Missouri, Plant Sci Div, Columbia, MO 65211 USA. EM Hari.Krishnan@ARS.USDA.GOV FU United Soybean Board [1420-632-6605] FX This study was partially funded by a grant from the United Soybean Board (project number 1420-632-6605). The authors acknowledge T. Anai and Legumebase for providing lines MM106, M25, and KK2. Mention of a trademark, vendor, or proprietary product does not constitute a guarantee or warranty of the product by the United States Department of Agriculture (USDA) and does not imply its approval to the exclusion of other products or vendors that may also be suitable. The USDA Agricultural Research Service, Midwest Area is an equal opportunity, affirmative action employer and all agency services are available without discrimination. NR 60 TC 1 Z9 1 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0894-0282 EI 1943-7706 J9 MOL PLANT MICROBE IN JI Mol. Plant-Microbe Interact. PD NOV PY 2016 VL 29 IS 11 BP 862 EP 877 DI 10.1094/MPMI-08-16-0173-R PG 16 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences GA EF4IM UT WOS:000390289000004 PM 27749147 ER PT J AU Frihart, CR Coolidge, T Mock, C Valle, E AF Frihart, Charles R. Coolidge, Thomas Mock, Chera Valle, Eder TI High Bonding Temperatures Greatly Improve Soy Adhesive Wet Strength SO POLYMERS LA English DT Article DE soy; flour; concentrate; isolate; wet shear strength; bonding temperature ID PROTEIN ADHESIVE; FLOUR; FIBER AB Soy wood adhesive bond strengths reported in different literature studies are difficult to compare because a variety of temperatures and other conditions have been used for the bonding and testing step. Some reports have indicated bond strengths are sensitive to bonding temperature, but the reason(s) for this has not been intensively investigated. Although these prior studies differ in other ways (such as type of soy, wood species, and test method), the effect of bonding temperature has not been clearly examined, which is important for focusing commercial applications. A tensile shear test using two-parallel-ply veneer specimens with smooth maple was used to measure both the dry and wet cohesive strength of soy adhesives. Although the soy adhesives gave very good strengths and dry wood failure, they often have low wood failure and shear strengths under wet conditions when bonded at 120 degrees C. However, wet strength greatly increased as the bonding temperature increased (120, 150 and 180 degrees C) for these two-ply tests with. This study examined the use of different types of soys (flours, concentrates and isolates) and different bonding temperatures and bonding conditions to evacuate several possible mechanisms for this temperature sensitivity, with coalescence being the most likely. C1 [Frihart, Charles R.] Forest Prod Lab, Madison, WI 53726 USA. [Coolidge, Thomas; Mock, Chera; Valle, Eder] AgriChem Technol, Mt Horeb, WI 53572 USA. RP Frihart, CR (reprint author), Forest Prod Lab, Madison, WI 53726 USA. EM cfrihart@fs.fed.us; thomas@agchemtech.com; chera33@yahoo.com; eder@agchemtech.com NR 25 TC 0 Z9 0 U1 5 U2 5 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2073-4360 J9 POLYMERS-BASEL JI Polymers PD NOV PY 2016 VL 8 IS 11 AR 394 DI 10.3390/polym8110394 PG 10 WC Polymer Science SC Polymer Science GA EF1TB UT WOS:000390106600017 ER PT J AU Ma, J Jacques, PF Hwang, SJ Troy, LM McKeown, NM Chu, AY Fox, CS AF Ma, Jiantao Jacques, Paul F. Hwang, Shih-Jen Troy, Lisa M. McKeown, Nicola M. Chu, Audrey Y. Fox, Caroline S. TI Dietary Guideline Adherence Index and Kidney Measures in the Framingham Heart Study SO AMERICAN JOURNAL OF KIDNEY DISEASES LA English DT Article DE Dietary Guidelines for Americans Adherence Index (DGAI); meat and legumes; dairy products; estimated glomerular filtration rate (eGFR); incident low eGFR; rapid eGFR decline; renal function; kidney disease progression; modifiable risk factor; diet; Framingham Heart Study Offspring Cohort; albuminuria; urinary albumin-creatinine ratio (UACR) ID FOOD-FREQUENCY QUESTIONNAIRE; CARDIOVASCULAR-DISEASE; RENAL-FUNCTION; FUNCTION DECLINE; SWEETENED SODA; PROTEIN-INTAKE; RISK-FACTOR; ASSOCIATIONS; REPRODUCIBILITY; ALBUMINURIA AB Background: No observational studies have directly considered dietary guidelines when examining the prospective association between dietary intake and kidney measures. Study Design: Prospective cohort study. Setting & Participants: We examined participants who attended examinations 7 (1998-2001) and 8 (2005-2008) in the Framingham Offspring Cohort. Predictors: Individual components of Dietary Guidelines for Americans Adherence Index (DGAI) that reflect adherence to key dietary recommendations based on the 2005 guideline. Outcomes & Measures: The primary outcome was incident low estimated glomerular filtration rate (eGFR) at follow-up after exclusion of prevalent low eGFR at baseline. Low eGFR was defined as serum creatinine-based eGFR <60 mL/min/1.73 m(2). Results: Among 1,822 participants (mean age, 59.4 years; 54.6% women), 181 incident cases of low eGFR were identified. After adjustment for potential confounders, compared to optimal adherence to meat and legume recommendations, low adherence was associated with higher odds of incident low eGFR (P for trend = 0.01); ORs in the lowest and intermediate adherence categories were 2.98 (95% CI, 1.13-7.92) and 1.65 (95% CI, 1.02-2.66), respectively. Low adherence to dairy product recommendations was also associated with higher odds of incident low eGFR compared to optimal adherence (P for trend = 0.03); ORs in the lowest and intermediate adherence categories were 1.98 (95% CI, 1.03-3.82) and 1.59 (95% CI, 0.81-3.11), respectively. In addition, low adherence to meat and legume recommendations was associated with rapid eGFR decline (P for trend = 0.01), and low adherence to dairy product recommendations was associated with rapid eGFR decline (P for trend = 0.01) and incident albuminuria (P for trend = 0.03). Limitations: The DGAI was developed based on the 2005 Dietary Guidelines for Americans. Conclusions: Better adherence to dietary recommendations for both meat and legumes and dairy products was associated with lower risk for developing adverse kidney measures. Published by Elsevier Inc. on behalf of the National Kidney Foundation, Inc. C1 [Ma, Jiantao; Hwang, Shih-Jen; Chu, Audrey Y.; Fox, Caroline S.] NHLBI, Framingham Heart Study & Populat Sci Branch, 73 Mt Wayte Ave,Ste 2, Framingham, MA 01702 USA. [Jacques, Paul F.; McKeown, Nicola M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Epidemiol Program, Boston, MA 02111 USA. [Troy, Lisa M.] Univ Massachusetts, Dept Nutr, Amherst, MA 01003 USA. RP Fox, CS (reprint author), NHLBI, Framingham Heart Study & Populat Sci Branch, 73 Mt Wayte Ave,Ste 2, Framingham, MA 01702 USA. EM foxca@nhlbi.nih.gov FU NHLBI Framingham Heart Study [N01-HC-25195] FX This work was conducted in part using resources and data from the Framingham Heart Study of the National Heart, Lung and Blood Institute (NHLBI) of the National Institutes of Health (NIH) and Boston University School of Medicine. This work was supported by the NHLBI Framingham Heart Study (N01-HC-25195). The funders of this study had no role in study design; collection, analysis, and interpretation of data; writing the report; and the decision to submit the report for publication. The views expressed in this manuscript are those of the authors and do not necessarily represent the views of the NHLBI, the NIH, or the US Department of Health and Human Services. NR 30 TC 1 Z9 1 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0272-6386 EI 1523-6838 J9 AM J KIDNEY DIS JI Am. J. Kidney Dis. PD NOV PY 2016 VL 68 IS 5 BP 703 EP 715 DI 10.1053/j.ajkd.2016.04.015 PG 13 WC Urology & Nephrology SC Urology & Nephrology GA EE3QT UT WOS:000389511300010 PM 27261331 ER PT J AU Crossman, J Eimers, MC Casson, NJ Burns, DA Campbell, JL Likens, GE Mitchell, MJ Nelson, SJ Shanley, JB Watmough, SA Webster, KL AF Crossman, Jill Eimers, M. Catherine Casson, Nora J. Burns, Douglas A. Campbell, John L. Likens, Gene E. Mitchell, Myron J. Nelson, Sarah J. Shanley, James B. Watmough, Shaun A. Webster, Kara L. TI Regional meteorological drivers and long term trends of winter-spring nitrate dynamics across watersheds in northeastern North America SO BIOGEOCHEMISTRY LA English DT Article DE Rain-on-snow; Nitrate; Meteorological drivers ID SOUTH-CENTRAL ONTARIO; ON-SNOW EVENTS; WESTERN UNITED-STATES; FORESTED CATCHMENTS; BASE-CATION; CATSKILL MOUNTAINS; NITROGEN BUDGETS; FUTURE CHANGES; NEW-HAMPSHIRE; MAINE BBWM AB This study evaluated the contribution of winter rain-on-snow (ROS) events to annual and seasonal nitrate (N-NO3) export and identified the regional meteorological drivers of inter-annual variability in ROS N-NO3 export (ROS-N) at 9 headwater streams located across Ontario, Canada and the northeastern United States. Although on average only 3.3 % of annual precipitation fell as ROS during winter over the study period, these events contributed a significant proportion of annual and winter N-NO3 export at the majority of sites (average of 12 and 42 %, respectively); with the exception of the most northern catchment, where total winter precipitation was exceptionally low (average 77 mm). In years with a greater magnitude of ROS events, the timing of the peak N-NO3 export period (during spring melt) was redistributed to earlier in the year. Variability in ROS frequency and magnitude amongst sites was high and a generalised linear model demonstrated that this spatial variability could be explained by interactive effects between regional and site-specific drivers. Snowpack coverage was particularly important for explaining the site-specific ROS response. Specifically, ROS events were less common when higher temperatures eliminated snow cover despite increasing the proportion of winter rainfall, whereas ROS event frequency was greater at sites where sufficient snow cover remained. This research suggests that catchment response to changes in N deposition is sensitive to climate change; a vulnerability which appears to vary in intensity throughout the seasonally snow-covered temperate region. Furthermore, the sensitivity of stream N-NO3 export to ROS events and potential shifts (earlier) in the timing of N-NO3 export relative to other nutrients affect downstream nutrient stoichiometry and the community composition of phytoplankton and other algae. C1 [Crossman, Jill; Eimers, M. Catherine] Trent Univ, Sch Environm, West Bank Dr, Peterborough, ON K9J 7B8, Canada. [Casson, Nora J.] Univ Winnipeg, Dept Geog, Portage Ave, Winnipeg, MB R3B 2E9, Canada. [Burns, Douglas A.] US Geol Survey, New York Water Sci Ctr, 425 Jordan Rd, New York, NY 12180 USA. [Campbell, John L.] USDA Forest Serv, Northern Res Stn, 271 Mast Rd, Durham, NH 03824 USA. [Likens, Gene E.] Cary Inst Ecosyst Studies, Millbrook, NY 12545 USA. [Likens, Gene E.] Univ Connecticut, Dept Ecol & Evolutionary Biol, Storrs, CT 06269 USA. [Mitchell, Myron J.] SUNY Coll Environm Sci & Forestry, Syracuse, NY 13210 USA. [Nelson, Sarah J.] Univ Maine, Sch Forest Resources, 5755 Nutting Hall, Orono, ME 04469 USA. [Shanley, James B.] US Geol Survey, New England Water Sci Ctr, 331 Commerce Way, Pembroke, NH 03275 USA. [Watmough, Shaun A.] Trent Univ, Environm & Resource Studies Program, Peterborough, ON K9J 7B8, Canada. [Webster, Kara L.] Canadian Forest Serv, Nat Resources Canada, 1219 Queen St, Sault Ste Marie P6A 2E5, ON, Canada. RP Crossman, J (reprint author), Trent Univ, Sch Environm, West Bank Dr, Peterborough, ON K9J 7B8, Canada. EM jillcrossman@trentu.ca FU NSERC; National Science Foundation; A.W. Mellon Foundation; Long-Term Monitoring Program of the U.S. Environmental Protection Agency; Canadian Forest Service FX The authors thank the many agencies that contributed to the long-term data records for these sites. The cross-site comparison was funded by an NSERC Strategic Grant. Monitoring of Harp and Dickie Lake was supported by the Ontario Ministry of Environment and Climate Change, and the Dorset Environmental Science Centre. The long-term data from IISD-ELA represent the hard work of many past and present staff, and are gratefully acknowledged. BBWM data have been collected since 1986 with support from the US EPA, US NSF, USGS, USFS, the Maine Agricultural and Forest Experiment Station, and the University of Maine. Funding for precipitation and streamwater chemistry at Hubbard Brook was provided by the National Science Foundation, including the LTER and LTERB programs, and the A.W. Mellon Foundation; hydrometeorological data were obtained through support from the USDA Forest Service. Data collection at Biscuit Brook is funded by the Long-Term Monitoring Program of the U.S. Environmental Protection Agency. Funding for this research at the TLW sites was provided by Canadian Forest Service base funds. NR 57 TC 0 Z9 0 U1 3 U2 3 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-2563 EI 1573-515X J9 BIOGEOCHEMISTRY JI Biogeochemistry PD NOV PY 2016 VL 130 IS 3 BP 247 EP 265 DI 10.1007/s10533-016-0255-z PG 19 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA EF0NG UT WOS:000390021800004 ER PT J AU Das, S Roberts, S Ravussin, E Holloszy, J Hadley, E Romashkan, S Kraus, W AF Das, S. Roberts, S. Ravussin, E. Holloszy, J. Hadley, E. Romashkan, S. Kraus, W. TI FINDINGS FROM A TWO-YEAR RANDOMIZED CONTROLLED TRIAL OF HUMAN CALORIC RESTRICTION SO GERONTOLOGIST LA English DT Meeting Abstract C1 [Das, S.; Roberts, S.] Tufts Univ, Energy Metab, Jean Mayer USDA, HNRCA, Boston, MA 02111 USA. [Ravussin, E.] PBRC, Baton Rouge, LA USA. [Holloszy, J.] Washington Univ, St Louis, MO USA. [Hadley, E.; Romashkan, S.] NIA, NIH, Bethesda, MD 20892 USA. [Kraus, W.] Duke Univ, Durham, NC USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0016-9013 EI 1758-5341 J9 GERONTOLOGIST JI Gerontologist PD NOV PY 2016 VL 56 SU 3 BP 497 EP 497 PG 1 WC Gerontology SC Geriatrics & Gerontology GA ED1DT UT WOS:000388585002324 ER PT J AU Sivakoff, FS Jones, SC Machtley, SA Hagler, JR AF Sivakoff, Frances S. Jones, Susan C. Machtley, Scott A. Hagler, James R. TI Protein Self-Marking by Ectoparasites: A Case Study Using Bed Bugs (Hemiptera: Cimicidae) SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE Cimex lectularius; dispersal; immunomarking; mark-release-recapture ID CIMEX-LECTULARIUS; BEHAVIOR; DISPERSAL; PREDATORS; MOVEMENT; POPULATIONS; CONFLICT; DYNAMICS; INSECTS; DISEASE AB The ability to mark individuals is a critical feature of many entomological investigations, including dispersal studies. Insect dispersal is generally investigated using mark-release-recapture techniques, whereby marked individuals are released at a known location and then captured at a measured distance. Ectoparasite dispersal has historically been challenging to study, in part because of the ethical concerns associated with releasing marked individuals. Here, we introduce the protein self-marking technique, whereby ectoparasites mark themselves in the field by feeding on the blood of an introduced host. We demonstrate the potential of this technique using laboratory-reared bed bugs (Cimex lectularius L.) that marked themselves by feeding on either rabbit or chicken blood. We then used enzyme-linked immunosorbent assays to detect host-specific blood serum proteins in bed bugs. We assessed these protein markers' ability to 1) distinctively identify marked individuals, 2) persist following multiple feedings on an alternate diet, 3) persist over time across a range of temperatures, and 4) transfer from marked to unmarked individuals. Protein markers were detectable in bed bugs before and after molting, remained detectible after multiple feedings on an alternate diet, persisted regardless of whether an individual was starved or fed on an alternate diet following original mark acquisition, and did not transfer between individuals. The duration of detectability depended on temperature. Our results suggest that protein self-marking is an effective technique for marking bed bugs and holds promise for use in dispersal studies of ectoparasitic insects. C1 [Sivakoff, Frances S.; Jones, Susan C.] Ohio State Univ, Dept Entomol, Rothenbuhler Res Lab, 2501 Carmack Rd, Columbus, OH 43210 USA. [Machtley, Scott A.; Hagler, James R.] USDA ARS, Arid Land Agr Res Ctr, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. RP Sivakoff, FS (reprint author), Ohio State Univ, Dept Entomol, Rothenbuhler Res Lab, 2501 Carmack Rd, Columbus, OH 43210 USA. EM sivakoff.3@osu.edu; jones.1800@osu.edu; Scott.Machtley@ARS.USDA.GOV; James.Hagler@ARS.USDA.GOV NR 48 TC 0 Z9 0 U1 6 U2 6 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-2585 EI 1938-2928 J9 J MED ENTOMOL JI J. Med. Entomol. PD NOV PY 2016 VL 53 IS 6 BP 1370 EP 1377 DI 10.1093/jme/tjw117 PG 8 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA EF3IH UT WOS:000390217500014 PM 27493250 ER PT J AU Bara, JJ Parker, AT Muturi, EJ AF Bara, Jeffrey J. Parker, Allison T. Muturi, Ephantus J. TI Comparative Susceptibility of Ochlerotatus japonicus, Ochlerotatus triseriatus, Aedes albopictus, and Aedes aegypti (Diptera: Culicidae) to La Crosse Virus SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE arboviral transmission; vector competence; vector-borne pathogen; invasive species; mosquito-borne disease ID VECTOR COMPETENCE; UNITED-STATES; INVASIVE MOSQUITOS; EASTERN TENNESSEE; BODY-SIZE; ENCEPHALITIS; INFECTION; TRANSMISSION; USA; POPULATION AB Invasive mosquito species can increase the transmission risk of native mosquito-borne diseases by acting as novel vectors. In this study, we examined the susceptibility of three exotic invasive mosquito species Aedes aegypti (L.), Ae. albopictus (Skuse), and Ochlerotatus japonicus (Theobald) to La Crosse virus (LACV) relative to the native primary vector Ochlerotatus triseriatus (Say). Adult females of the four mosquito species were orally challenged with LACV; incubated for 3, 5, 7, 9, or 11 d; and their midgut infection rates, dissemination rates, and effective vector competence were determined. Overall, Oc. japonicus (2.92) had the highest effective vector competence values, followed by Ae. albopictus (1.55), Ae. aegypti (0.88), and Oc. triseriatus (0.64). In addition, we assessed the relationship between mosquito size and LACV susceptibility for field-collected Oc. triseriatus and Oc. japonicus. We hypothesized that smaller adults would be more susceptible to LACV; however, our results did not support this hypothesis. Infected Oc. triseriatus tended to be larger than exposed but uninfected females, while infected and uninfected Oc. japonicus were similarly sized. These findings suggest that Oc. japonicus, Ae. albopictus, and Ae. aegypti have significant potential to transmit LACV and more research is needed to uncover their potential role in LACV epidemiology. C1 [Bara, Jeffrey J.] Univ Louisville, Dept Biol, Louisville, KY 40209 USA. [Bara, Jeffrey J.; Parker, Allison T.; Muturi, Ephantus J.] Univ Illinois, Illinois Nat Hist Survey, Champaign, IL 61820 USA. [Muturi, Ephantus J.] ARS, Crop Bioprotect Res Unit, USDA, 1815 N Univ St, Peoria, IL 61604 USA. RP Bara, JJ (reprint author), Univ Louisville, Dept Biol, Louisville, KY 40209 USA.; Bara, JJ (reprint author), Univ Illinois, Illinois Nat Hist Survey, Champaign, IL 61820 USA. EM jay.bara@gmail.com; allison.t.parker@gmail.com; Ephantus.Muturi@ars.usda.gov FU State of Illinois FX We thank Chung-Hyun Kim, Michael Kozak, and Andrew Donelson for their assistance in mosquito collection, identification, and daily maintenance of the experiment and Dr. Robert Tesh and the University of Texas Medical Branch World Reference Center for Emerging Viruses and Arboviruses for providing the LACV strain used in this study. We are grateful to Allison Gardner for her helpful comments on the manuscript and to Susanna Remold for her help in calculating EVC. This study was supported by the Used Tire Fund and Emergency Public Health Fund from the State of Illinois. NR 48 TC 0 Z9 0 U1 6 U2 6 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-2585 EI 1938-2928 J9 J MED ENTOMOL JI J. Med. Entomol. PD NOV PY 2016 VL 53 IS 6 BP 1415 EP 1421 DI 10.1093/jme/tjw097 PG 7 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA EF3IH UT WOS:000390217500021 PM 27605372 ER PT J AU Masi, M Cala, A Tabanca, N Cimmino, A Green, IR Bloomquist, JR van Otterlo, WAL Macias, FA Evidente, A AF Masi, Marco Cala, Antonio Tabanca, Nurhayat Cimmino, Alessio Green, Ivan R. Bloomquist, Jeffrey R. van Otterlo, Willem A. L. Macias, Francisco A. Evidente, Antonio TI Alkaloids with Activity against the Zika Virus Vector Aedes aegypti (L.)-Crinsarnine and Sarniensinol, Two New Crinine and Mesembrine Type Alkaloids Isolated from the South African Plant Nerine sarniensis SO MOLECULES LA English DT Article DE Nerine sarniensis; Amaryllidaceae; mesembrine; crinine; crinsarnine; sarniensinol; bowdensine; Zika virus; Aedes aegypti ID AMARYLLIDACEAE AB Two new Amaryllidaceae alkaloids, belonging to the mesembrine-and crinine-types, named crinsarnine (1) and sarniensinol (2), were isolated from the dried bulbs of Nerine sarniensis together with bowdensine (3), sarniensine (4), hippadine (5) and 1-O-acetyl-lycorine (6). Crinsarnine (1) and sarniensinol (2) were characterized using spectroscopic and chiroptical methods as (1S,2S,4aR,10bS)-2,7-dimethoxy-1,2,3,4,4a, 6-hexahydro-5,11b-ethano[1,3]dioxolo-[4,5-j]phenanthridin-1-yl acetate and (6-(3aR,4Z,6S,7aS)-6-methoxy-1-methyl-2,3,3a, 6,7,7a-hexa-hydro-1H-indol-3a-yl)benzo [d][1,3]dioxol-5-yl)methanol, respectively. Furthermore, the complete spectroscopic characterization of bowdensine (3) is reported for the first time. Compounds 1-6 were evaluated against the Orlando reference strain of Aedes aegypti. None of compounds showed mortality against 1st instar Ae. aegypti larvae at the concentrations tested. In adult topical bioassays, only 1 displayed adulticidal activity with an LD50 = 2.29 +/- 0.049 mu g/mosquito. As regards the structure-activity relationship, the pretazettine and crinine scaffold in 2 and 4 and in 1 and 3 respectively, proved to be important for their activity, while the pyrrole[de]phenanthridine scaffold present in 5 and 6 was important for their reactivity. Among the pretazettine group compounds, opening of the B ring or the presence of a B ring lactone as well as the trans-stereochemistry of the A/B ring junction, appears to be important for activity, while in crinine-type alkaloids, the substituent at C-2 seems to play a role in their activity. C1 [Masi, Marco; Cala, Antonio; Cimmino, Alessio; Evidente, Antonio] Univ Naples Federico II, Dept Chem Sci, Complesso Univ Monte S Angelo,Via Cintia 4, I-80126 Naples, Italy. [Cala, Antonio; Macias, Francisco A.] Univ Cadiz, Allelopathy Grp, Dept Organ Chem, Sch Sci,Inst Biomol INBIO, C Republ Saharaui 7, Cadiz 11510, Spain. [Tabanca, Nurhayat; Bloomquist, Jeffrey R.] Univ Florida, Dept Entomol & Nematol, Emerging Pathogens Inst, Gainesville, FL 32610 USA. [Tabanca, Nurhayat; van Otterlo, Willem A. L.] ARS, USDA, Subtrop Hort Res Stn, 13601 Old Cutler Rd, Miami, FL 33158 USA. [Green, Ivan R.] Univ Stellenbosch, Dept Chem & Polymer Sci, Private Bag X1, ZA-7602 Stellenbosch, South Africa. RP Evidente, A (reprint author), Univ Naples Federico II, Dept Chem Sci, Complesso Univ Monte S Angelo,Via Cintia 4, I-80126 Naples, Italy. EM marco.masi@unina.it; antonio.cala@uca.es; ntabanca@ufl.edu@ufl.edu; alessio.cimmino@unina.it; irg@sun.ca.za; jbquist@epi.ufl.edu; wvo@sun.ca.za; famacias@uca.es; evidente@unina.it RI Cimmino, Alessio/I-2121-2012; OI Cimmino, Alessio/0000-0002-1551-4237; Cala, Antonio/0000-0001-5714-4556; MASI, Marco/0000-0003-0609-8902 FU Deployed War-Fighter Protection Research Program via U.S. Department of Defense through the Armed Forces Pest Management Board; Research Unit of the University of Cadiz in the Researchers Training Program; Italian and South-African Ministry of Foreign Affairs Area: South Africa, Medicine and Health [ZA14MO06]; National Research Foundation-South Africa (NRF); NRF; Stellenbosch University (Faculty of Science and University Research Office) FX This study was partly funded by the Deployed War-Fighter Protection Research Program via grants from the U.S. Department of Defense through the Armed Forces Pest Management Board (to Jeffrey R. Bloomquist, Department of Entomology and Nematology, Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610, USA). A.C. (Spain) thanks the financial support of the Research Unit of the University of Cadiz in the Researchers Training Program. We thank James J. Becnel, Alden S. Estep, Jessica Louton, (USDA-ARS, CMAVE, Gainesville, FL, USA) for mosquito bioassays. Part of the work was supported by grants from a bilateral project of the Italian and South-African Ministry of Foreign Affairs Area: South Africa, Medicine and Health Project number: ZA14MO06, titled: Bioactive metabolites from South-Africa plants with anticancer activity. This South African part of this project was overseen by the National Research Foundation-South Africa (NRF) and their support is gratefully acknowledged. Willem A. L. van Otterlo and Ivan R. Green acknowledge support from the NRF (Incentive funding grants) and Stellenbosch University (Faculty of Science and University Research Office). Martin Smit, curator of the Stellenbosch Botanical Garden is thanked for the allocation of space for the three living specimens of Nerine sarniensis. A.E. is associated to the Istituto di Chimica Biomolecolare del CNR, Pozzuoli, Italy. NR 28 TC 0 Z9 0 U1 4 U2 4 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1420-3049 J9 MOLECULES JI Molecules PD NOV PY 2016 VL 21 IS 11 AR 1432 DI 10.3390/molecules21111432 PG 11 WC Chemistry, Organic SC Chemistry GA EE9AO UT WOS:000389918200016 ER PT J AU Zhan, ZY Chen, YC Shockey, J Han, XJ Wang, YD AF Zhan, Zhiyong Chen, Yicun Shockey, Jay Han, Xiaojiao Wang, Yangdong TI Proteomic Analysis of Tung Tree (Vernicia fordii) Oilseeds during the Developmental Stages SO MOLECULES LA English DT Article DE tung tree; seed; oil content; oil body; development; proteomics ID 2-DIMENSIONAL GEL-ELECTROPHORESIS; SAMPLE EXTRACTION TECHNIQUES; RECALCITRANT PLANT-TISSUES; PROTEIN EXTRACTION; SEED-GERMINATION; BRASSICA-NAPUS; OIL BODIES; MEMBRANE-PROTEINS; JATROPHA-CURCAS; EXPRESSION AB The tung tree (Vernicia fordii), a non-model woody plant belonging to the Euphorbiaceae family, is a promising economic plant due to the high content of a novel high-value oil in its seeds. Many metabolic pathways are active during seed development. Oil (triacylglycerols (TAGs)) accumulates in oil bodies distributed in the endosperm cells of tung tree seeds. The relationship between oil bodies and oil content during tung tree seed development was analyzed using ultrastructural observations, which confirmed that oil accumulation was correlated with the volumes and numbers of oil bodies in the endosperm cells during three different developmental stages. For a deeper understanding of seed development, we carried out proteomic analyses. At least 144 proteins were differentially expressed during three different developmental stages. A total of 76 proteins were successfully identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry/ mass spectrometry (MALDI-TOF/MS/MS). These proteins were grouped into 11 classes according to their functions. The major groups of differentially expressed proteins were associated with energy metabolism (25%), fatty acid metabolism (15.79%) and defense (14.47%). These results strongly suggested that a very high percentage of gene expression in seed development is dedicated to the synthesis and accumulation of TAGs. C1 [Zhan, Zhiyong; Chen, Yicun; Han, Xiaojiao; Wang, Yangdong] Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China. [Zhan, Zhiyong; Chen, Yicun; Han, Xiaojiao; Wang, Yangdong] Chinese Acad Forestry, Res Inst Subtrop Foresty, Fuyang 311400, Zhejiang, Peoples R China. [Zhan, Zhiyong] Jiangxi Acad Forestry, Nanchang 330013, Jiangxi, Peoples R China. [Shockey, Jay] USDA ARS, Southern Reg Res Ctr, New Orleans, LA 70124 USA. RP Wang, YD (reprint author), Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China.; Wang, YD (reprint author), Chinese Acad Forestry, Res Inst Subtrop Foresty, Fuyang 311400, Zhejiang, Peoples R China. EM zhanzhiyong862007@163.com; yicun_chen@163.com; jay.shockey@ars.usda.gov; hanxiaojiao1004@163.com; wyd11111@126.com FU Zhejiang Province Natural Science Foundation of China [LZ13C160001] FX This work was supported financially by Zhejiang Province Natural Science Foundation of China (LZ13C160001). NR 47 TC 0 Z9 0 U1 5 U2 5 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1420-3049 J9 MOLECULES JI Molecules PD NOV PY 2016 VL 21 IS 11 AR 1486 DI 10.3390/molecules21111486 PG 11 WC Chemistry, Organic SC Chemistry GA EE9AO UT WOS:000389918200068 ER PT J AU Novick, KA Ficklin, DL Stoy, PC Williams, CA Bohrer, G Oishi, AC Papuga, SA Blanken, PD Noormets, A Sulman, BN Scott, RL Wang, LX Phillips, RP AF Novick, Kimberly A. Ficklin, Darren L. Stoy, Paul C. Williams, Christopher A. Bohrer, Gil Oishi, A. Christopher Papuga, Shirley A. Blanken, Peter D. Noormets, Asko Sulman, Benjamin N. Scott, Russell L. Wang, Lixin Phillips, Richard P. TI The increasing importance of atmospheric demand for ecosystem water and carbon fluxes SO NATURE CLIMATE CHANGE LA English DT Article ID MODELING STOMATAL CONDUCTANCE; VAPOR-PRESSURE DEFICIT; FOREST; EXCHANGE; CLIMATE; LAND; SOIL; EVAPOTRANSPIRATION; PHOTOSYNTHESIS; TRANSPIRATION AB Soil moisture supply and atmospheric demand for water independently limit-and profoundly affect-vegetation productivity and water use during periods of hydrologic stress(1-4). Disentangling the impact of these two drivers on ecosystem carbon and water cycling is difficult because they are often correlated, and experimental tools for manipulating atmospheric demand in the field are lacking. Consequently, the role of atmospheric demand is often not adequately factored into experiments or represented in models(5-7). Here we show that atmospheric demand limits surface conductance and evapotranspiration to a greater extent than soil moisture in many biomes, including mesic forests that are of particular importance to the terrestrial carbon sink(8,9). Further, using projections from ten general circulation models, we show that climate change will increase the importance of atmospheric constraints to carbon and water fluxes in all ecosystems. Consequently, atmospheric demand will become increasingly important for vegetation function, accounting for >70% of growing season limitation to surface conductance in mesic temperate forests. Our results suggest that failure to consider the limiting role of atmospheric demand in experimental designs, simulation models and land management strategies will lead to incorrect projections of ecosystem responses to future climate conditions. C1 [Novick, Kimberly A.] Indiana Univ, Sch Publ & Environm Affairs, Bloomington, IN 47405 USA. [Ficklin, Darren L.] Indiana Univ, Dept Geog, Bloomington, IN 47405 USA. [Stoy, Paul C.] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. [Williams, Christopher A.] Clark Univ, Grad Sch Geog, Worcester, MA 01610 USA. [Bohrer, Gil] Ohio State Univ, Dept Civil Environm & Geodet Engn, Columbus, OH 43210 USA. [Oishi, A. Christopher] US Forest Serv, USDA, Southern Res Stn, Coweeta Hydrol Lab, Otto, NC 28763 USA. [Papuga, Shirley A.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ 85721 USA. [Blanken, Peter D.] Univ Colorado, Dept Geog, Boulder, CO 80309 USA. [Noormets, Asko] North Carolina State Univ, Dept Forestry & Nat Resources, Raleigh, NC 27695 USA. [Sulman, Benjamin N.] Princeton Univ, Dept Geosci, Princeton, NJ 08544 USA. [Scott, Russell L.] USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ 85719 USA. [Wang, Lixin] Indiana Univ Purdue Univ, Dept Earth Sci, Indianapolis, IN 46202 USA. [Phillips, Richard P.] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA. RP Novick, KA (reprint author), Indiana Univ, Sch Publ & Environm Affairs, Bloomington, IN 47405 USA. EM knovick@indiana.edu FU US Department of Energy; National Science Foundation (NSF) [DEB 1552747]; NSF [DEB 1552976, EF 1241881, EAR 125501, EAR 155489]; NOAA/GFDL-Princeton University Cooperative Institute for Climate Science FX We acknowledge the US Department of Energy for its support of the Ameriflux Management Project administered by Lawrence Berkeley National Lab, and for its support of the Climate Model Diagnosis and Intercomparison Project. We thank the Ameriflux site teams for sharing their data, and the individual climate modelling groups for sharing their model output. We acknowledge the World Climate Research Programme's Working Group on Coupled Modelling, which is responsible for the Coupled Model Intercomparison Project (CMIP). K.A.N. acknowledges National Science Foundation (NSF) grant DEB 1552747. P.C.S. acknowledges NSF grants DEB 1552976 and EF 1241881. S.A.P. acknowledges NSF grant EAR 125501. L.W. acknowledges NSF grant EAR 155489. B.N.S. acknowledges support from NOAA/GFDL-Princeton University Cooperative Institute for Climate Science. NR 30 TC 2 Z9 2 U1 23 U2 23 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1758-678X EI 1758-6798 J9 NAT CLIM CHANGE JI Nat. Clim. Chang. PD NOV PY 2016 VL 6 IS 11 BP 1023 EP 1027 DI 10.1038/NCLIMATE3114 PG 5 WC Environmental Sciences; Environmental Studies; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA EE2QC UT WOS:000389428300017 ER PT J AU Edwards, JV Fontenot, KR Prevost, NT Pircher, N Liebner, F Condon, BD AF Edwards, J. Vincent Fontenot, Krystal R. Prevost, Nicolette T. Pircher, Nicole Liebner, Falk Condon, Brian D. TI Preparation, Characterization and Activity of a Peptide-Cellulosic Aerogel Protease Sensor from Cotton SO SENSORS LA English DT Article DE elastase peptide; cellulosic aerogels; human neutrophil elastase; biosensor; chronic wounds ID ATOMIC-FORCE MICROSCOPY; ROOM-TEMPERATURE; SURFACE-AREA; WOUND FLUID; CARBON; PECTIN; NANOCRYSTALS; SPECTROSCOPY; DRESSINGS; PERFORMANCE AB Nanocellulosic aerogels (NA) provide a lightweight biocompatible material with structural properties, like interconnected high porosity and specific surface area, suitable for biosensor design. We report here the preparation, characterization and activity of peptide-nanocellulose aerogels (PepNA) made from unprocessed cotton and designed with protease detection activity. Low-density cellulosic aerogels were prepared from greige cotton by employing calcium thiocyanate octahydrate /lithium chloride as a direct cellulose dissolving medium. Subsequent casting, coagulation, solvent exchange and supercritical carbon dioxide drying afforded homogeneous cellulose II aerogels of fibrous morphology. The cotton-based aerogel had a porosity of 99% largely dominated by mesopores (2-50 nm) and an internal surface of 163 m(2).g(-1). A fluorescent tripeptide-substrate (succinyl-alanine-proline-alanine-4-amino-7-methyl-coumarin) was tethered to NA by (1) esterification of cellulose C6 surface hydroxyl groups with glycidyl-fluorenylmethyloxycarbonyl (FMOC), (2) deprotection and (3) coupling of the immobilized glycine with the tripeptide. Characterization of the NA and PepNA included techniques, such as elemental analysis, mass spectral analysis, attenuated total reflectance infrared imaging, nitrogen adsorption, scanning electron microscopy and bioactivity studies. The degree of substitution of the peptide analog attached to the anhydroglucose units of PepNA was 0.015. The findings from mass spectral analysis and attenuated total reflectance infrared imaging indicated that the peptide substrate was immobilized on to the surface of the NA. Nitrogen adsorption revealed a high specific surface area and a highly porous system, which supports the open porous structure observed from scanning electron microscopy images. Bioactivity studies of PepNA revealed a detection sensitivity of 0.13 units/milliliter for human neutrophil elastase, a diagnostic biomarker for inflammatory diseases. The physical properties of the aerogel are suitable for interfacing with an intelligent protease sequestrant wound dressing. C1 [Edwards, J. Vincent; Fontenot, Krystal R.; Prevost, Nicolette T.; Condon, Brian D.] USDA, Southern Reg Res Ctr, New Orleans, LA 70124 USA. [Pircher, Nicole; Liebner, Falk] Univ Nat Resources & Life Sci Vienna, Div Chem Renewable Resources, Konrad Lorenz Str 24, A-3430 Tulin An Der Donau, Austria. RP Edwards, JV (reprint author), USDA, Southern Reg Res Ctr, New Orleans, LA 70124 USA. EM vince.edwards@ars.usda.gov; krystal.fontenot@ars.usda.gov; nicolette.prevost@ars.usda.gov; brian.condon@ars.usda.gov; falk.liebner@boku.ac.at; brian.condon@ars.usda.gov OI Liebner, Falk/0000-0002-8244-8153 FU U.S. Department of Agriculture; Austrian Science Fund FX This project was supported by the U.S. Department of Agriculture. We thank Casey Grimm for Mass Spectral analysis. The authors wish to thank Lawson Gary, Chief Operating Officer, T. J. Beall, Co. Inc., for the donation of True Cotton (TM) samples. Furthermore, the support of project CAPBONE by the Austrian Science Fund is thankfully acknowledged. NR 83 TC 0 Z9 0 U1 13 U2 13 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1424-8220 J9 SENSORS-BASEL JI Sensors PD NOV PY 2016 VL 16 IS 11 DI 10.3390/s16111789 PG 19 WC Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation SC Chemistry; Electrochemistry; Instruments & Instrumentation GA EE5JB UT WOS:000389641700022 ER PT J AU Pelletier, MG Wanjura, JD Holt, GA AF Pelletier, Mathew G. Wanjura, John D. Holt, Greg A. TI Microwave Moisture Sensing of Seedcotton: Part 1: Seedcotton Microwave Material Properties SO SENSORS LA English DT Article DE microwave; moisture; cotton; seedcotton; seed; density independent ID TIME-DOMAIN REFLECTOMETRY; PERMITTIVITY MEASUREMENTS; DIELECTRIC-PROPERTIES; FREE-SPACE; FREQUENCIES; SOLIDS; WHEAT; GRAIN AB Moisture content at harvest is a key parameter that impacts quality and how well the cotton crop can be stored without degrading before processing. It is also a key parameter of interest for harvest time field trials as it can directly influence the quality of the harvested crop as well as skew the results of in-field yield and quality assessments. Microwave sensing of moisture has several unique advantages over lower frequency sensing approaches. The first is that microwaves are insensitive to variations in conductivity, due to presence of salts or minerals. The second advantage is that microwaves can peer deep inside large bulk packaging to assess the internal moisture content without performing a destructive tear down of the package. To help facilitate the development of a microwave moisture sensor for seedcotton; research was performed to determine the basic microwave properties of seedcotton. The research was performed on 110 kg micro -modules, which are of direct interest to research teams for use in ongoing field -based research projects. It should also prove useful for the enhancement of existing and future yield monitor designs. Experimental data was gathered on the basic relations between microwave material properties and seedcotton over the range from 1.0 GHz to 2.5 GHz and is reported on herein. This research is part one of a two-part series that reports on the fundamental microwave properties of seedcotton as moisture and density vary naturally during the course of typical harvesting operations; part two will utilize this data to formulate a prediction algorithm to form the basis for a prototype microwave moisture sensor. C1 [Pelletier, Mathew G.; Wanjura, John D.; Holt, Greg A.] ARS, USDA, Cotton Prod & Proc Res Unit, Lubbock, TX 79403 USA. RP Pelletier, MG (reprint author), ARS, USDA, Cotton Prod & Proc Res Unit, Lubbock, TX 79403 USA. EM Mathew.Pelletier@ars.usda.gov; John.Wanjura@ars.usda.gov; Greg.Holt@ars.usda.gov NR 31 TC 0 Z9 0 U1 0 U2 0 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1424-8220 J9 SENSORS-BASEL JI Sensors PD NOV PY 2016 VL 16 IS 11 AR 1843 DI 10.3390/s16111843 PG 20 WC Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation SC Chemistry; Electrochemistry; Instruments & Instrumentation GA EE5JB UT WOS:000389641700076 ER PT J AU Snyder, KA Wehan, BL Filippa, G Huntington, JL Stringham, TK Snyder, DK AF Snyder, Keirith A. Wehan, Bryce L. Filippa, Gianluca Huntington, Justin L. Stringham, Tamzen K. Snyder, Devon K. TI Extracting Plant Phenology Metrics in a Great Basin Watershed: Methods and Considerations for Quantifying Phenophases in a Cold Desert SO SENSORS LA English DT Article DE StarDot cameras; PhenoCam network; pinyon and juniper; sagebrush steppe; semi-arid meadows; camera-based repeat digital photography ID DECIDUOUS BROADLEAF FOREST; DIGITAL REPEAT PHOTOGRAPHY; WESTERN NORTH-AMERICA; MONITORING VEGETATION; ECOLOGICAL APPLICATIONS; CLIMATE-CHANGE; NDVI; GRASSLAND; CAMERAS; SYSTEM AB Plant phenology is recognized as important for ecological dynamics. There has been a recent advent of phenology and camera networks worldwide. The established PhenoCam Network has sites in the United States, including the western states. However, there is a paucity of published research from semi-arid regions. In this study, we demonstrate the utility of camera-based repeat digital imagery and use of R statistical phenopix package to quantify plant phenology and phenophases in four plant communities in the semi-arid cold desert region of the Great Basin. We developed an automated variable snow/night filter for removing ephemeral snow events, which allowed fitting of phenophases with a double logistic algorithm. We were able to detect low amplitude seasonal variation in pinyon and juniper canopies and sagebrush steppe, and characterize wet and mesic meadows in area-averaged analyses. We used individual pixel-based spatial analyses to separate sagebrush shrub canopy pixels from interspace by determining differences in phenophases of sagebrush relative to interspace. The ability to monitor plant phenology with camera-based images fills spatial and temporal gaps in remotely sensed data and field based surveys, allowing species level relationships between environmental variables and phenology to be developed on a fine time scale thus providing powerful new tools for land management. C1 [Snyder, Keirith A.; Wehan, Bryce L.] USDA ARS, Great Basin Rangelands Res Unit, Reno, NV 89512 USA. [Filippa, Gianluca] ARPA Valle dAosta, Environm Protect Agcy Aosta Valley, Climate Change Unit, I-11020 St Christophe, Italy. [Huntington, Justin L.] Desert Res Inst, Western Reg Climate Ctr, Reno, NV 89512 USA. [Stringham, Tamzen K.; Snyder, Devon K.] Univ Nevada, Dept Agr Nutr & Vet Sci, Reno, NV 89557 USA. RP Snyder, KA (reprint author), USDA ARS, Great Basin Rangelands Res Unit, Reno, NV 89512 USA. EM keirith.snyder@ars.usda.gov; b.wehan@gmail.com; g.filippa@arpa.vda.it; justin.huntington@dri.edu; tstringham@cabnr.unr.edu; devonsnyder@cabnr.unr.edu OI Stringham, Tamzen/0000-0002-5851-0228 FU Great Basin Landscape Conservation Cooperative through the U.S. Fish and Wildlife Service [59-5370-3-001] FX Funding from the Great Basin Landscape Conservation Cooperative through the U.S. Fish and Wildlife Service received as U.S. Department of Agriculture Agricultural Research Service grant #59-5370-3-001. We thank Andrew Richardson from the PhenoCam Network for assistance with protocols; Sam Utley at Campbell Scientific for assistance with datalogger programming; and Anthony Watts at StarDot for assistance with camera setup; and three anonymous reviewers whose comments improved this manuscript. We gratefully acknowledge Smith Creek Ranch for access to the study area, Brian Minkin for image processing, and all those that helped with installation of the sensor network: Amira Morrow, Todd Adams, Ceasar Medina, Alex Clarke, Sandra Li and Ben Stringham. NR 57 TC 0 Z9 0 U1 4 U2 4 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1424-8220 J9 SENSORS-BASEL JI Sensors PD NOV PY 2016 VL 16 IS 11 AR 1948 DI 10.3390/s16111948 PG 20 WC Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation SC Chemistry; Electrochemistry; Instruments & Instrumentation GA EE5JB UT WOS:000389641700181 ER PT J AU Lorenz, RD Balme, MR Gu, ZL Kahanpaa, H Klose, M Kurgansky, MV Patel, MR Reiss, D Rossi, AP Spiga, A Takemi, T Wei, W AF Lorenz, Ralph D. Balme, Matthew R. Gu, Zhaolin Kahanpaa, Henrik Klose, Martina Kurgansky, Michael V. Patel, Manish R. Reiss, Dennis Rossi, Angelo Pio Spiga, Aymeric Takemi, Tetsuya Wei, Wei TI History and Applications of Dust Devil Studies SO SPACE SCIENCE REVIEWS LA English DT Review ID RESOLUTION STEREO CAMERA; AUSTRALIAN LANDSCAPE; WILLY-WILLIES; SOUTHERN PERU; MARS; VORTICES; TRACKS; VORTEX; CONVECTION; DESERT AB Studies of dust devils, and their impact on society, are reviewed. Dust devils have been noted since antiquity, and have been documented in many countries, as well as on the planet Mars. As time-variable vortex entities, they have become a cultural motif. Three major stimuli of dust devil research are identified, nuclear testing, terrestrial climate studies, and perhaps most significantly, Mars research. Dust devils present an occasional safety hazard to light structures and have caused several deaths. C1 [Lorenz, Ralph D.] Johns Hopkins Univ, Appl Phys Lab, Johns Hopkins Rd, Laurel, MD 20723 USA. [Balme, Matthew R.; Patel, Manish R.] Open Univ, Walton Hall, Milton Keynes MK7 6AA, Bucks, England. [Gu, Zhaolin] Xi An Jiao Tong Univ, Xian 710049, Shaanxi, Peoples R China. [Kahanpaa, Henrik] Finnish Meteorol Inst, FIN-00101 Helsinki, Finland. [Klose, Martina] Univ Cologne, Inst Geophys & Meteorol, Pohligstr 3, D-50969 Cologne, Germany. [Klose, Martina] USDA ARS Jornada Expt Range, Las Cruces, NM 88003 USA. [Kurgansky, Michael V.] Russian Acad Sci, AM Obukhov Inst Atmospher Phys, Pyzhevsky 3, Moscow 119017, Russia. [Patel, Manish R.] STFC Rutherford Appleton Lab, Space Sci & Technol Dept, Didcot OX11 0QX, Oxon, England. [Reiss, Dennis] Westfal Wilhelms Univ, Inst Planetol, Wilhelm Klemm Str 10, D-48149 Munster, Germany. [Rossi, Angelo Pio] Jacobs Univ Bremen, Dept Phys & Earth Sci, Campus Ring 1, D-28759 Bremen, Germany. [Spiga, Aymeric] LMD, 4 Pl Jussieu, F-75252 Paris 5, France. [Takemi, Tetsuya] Kyoto Univ, Disaster Prevent Res Inst, Uji, Kyoto 6110011, Japan. [Klose, Martina; Wei, Wei] Wuhan Univ Technol, Wuhan 430063, Hubei, Peoples R China. RP Lorenz, RD (reprint author), Johns Hopkins Univ, Appl Phys Lab, Johns Hopkins Rd, Laurel, MD 20723 USA. EM ralph.lorenz@jhuapl.edu RI Spiga, Aymeric/O-4858-2014; OI Spiga, Aymeric/0000-0002-6776-6268; Takemi, Tetsuya/0000-0002-7596-2373; Reiss, Dennis/0000-0002-1836-596X FU NASA Mars Fundamental Research Program [NNX12AI04G] FX The work of R. Lorenz was supported by NASA Mars Fundamental Research Program grant NNX12AI04G "Quantitative Study of Dust Devils". Several reviewers and reviewing editors provided copious improvements to the paper. NR 122 TC 3 Z9 3 U1 5 U2 5 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0038-6308 EI 1572-9672 J9 SPACE SCI REV JI Space Sci. Rev. PD NOV PY 2016 VL 203 IS 1-4 BP 5 EP 37 DI 10.1007/s11214-016-0239-2 PG 33 WC Astronomy & Astrophysics SC Astronomy & Astrophysics GA EE2LO UT WOS:000389415800002 ER PT J AU Spiga, A Barth, E Gu, ZL Hoffmann, F Ito, J Jemmett-Smith, B Klose, M Nishizawa, S Raasch, S Rafkin, S Takemi, T Tyler, D Wei, W AF Spiga, Aymeric Barth, Erika Gu, Zhaolin Hoffmann, Fabian Ito, Junshi Jemmett-Smith, Bradley Klose, Martina Nishizawa, Seiya Raasch, Siegfried Rafkin, Scot Takemi, Tetsuya Tyler, Daniel Wei, Wei TI Large-Eddy Simulations of Dust Devils and Convective Vortices SO SPACE SCIENCE REVIEWS LA English DT Review DE Dust devils; Large-Eddy Simulations; Convective vortices; Convective boundary layer ID PLANETARY BOUNDARY-LAYER; MARS ORBITER CAMERA; NUMERICAL-SIMULATION; ATMOSPHERIC CONVECTION; MODELING SYSTEM; FLUID-DYNAMICS; WRF MODEL; VORTEX; STATISTICS; TRANSPORT AB In this review, we address the use of numerical computations called Large-Eddy Simulations (LES) to study dust devils, and the more general class of atmospheric phenomena they belong to (convective vortices). We describe the main elements of the LES methodology. We review the properties, statistics, and variability of dust devils and convective vortices resolved by LES in both terrestrial and Martian environments. The current challenges faced by modelers using LES for dust devils are also discussed in detail. C1 [Spiga, Aymeric] UPMC Univ Paris 06, Sorbonne Univ, Inst Pierre Simon Laplace, UMR CNRS 8539,Lab Meteorol Dynam, Paris, France. [Barth, Erika; Rafkin, Scot] SouthWest Res Inst, Boulder, CO USA. [Gu, Zhaolin] Xi An Jiao Tong Univ, Xian, Peoples R China. [Hoffmann, Fabian; Raasch, Siegfried] Leibniz Univ Hannover, Inst Meteorol & Climatol, Hannover, Germany. [Ito, Junshi] Meteorol Res Inst, Ibaraki, Japan. [Jemmett-Smith, Bradley] Univ Leeds, Inst Climate & Atmospher Sci, Leeds, W Yorkshire, England. [Klose, Martina] Univ Cologne, Inst Geophys & Meteorol, Cologne, Germany. [Klose, Martina] USDA ARS Jornada Expt Range, Las Cruces, NM USA. [Nishizawa, Seiya] RIKEN Adv Inst Computat Sci, Kobe, Hyogo, Japan. [Takemi, Tetsuya] Kyoto Univ, Disaster Prevent Res Inst, Kyoto, Japan. [Tyler, Daniel] Oregon State Univ, Corvallis, OR 97331 USA. [Wei, Wei] Wuhan Univ Technol, Wuhan, Peoples R China. RP Spiga, A (reprint author), UPMC Univ Paris 06, Sorbonne Univ, Inst Pierre Simon Laplace, UMR CNRS 8539,Lab Meteorol Dynam, Paris, France. EM aymeric.spiga@upmc.fr RI Spiga, Aymeric/O-4858-2014; Nishizawa, Seiya/N-9689-2015; OI Spiga, Aymeric/0000-0002-6776-6268; Nishizawa, Seiya/0000-0001-9457-7457; Hoffmann, Fabian/0000-0001-5136-0653; Klose, Martina/0000-0001-8190-3700 FU International Space Science Institute (ISSI, Bern, Switzerland) FX We acknowledge the logistic and financial help of the International Space Science Institute (ISSI, Bern, Switzerland) for the organization of a "dust devils" international workshop that led to the writing of this review chapter. We are indebted to two anonymous reviewers and associate editor Ralph Lorenz for constructive comments which helped to improve this review. NR 96 TC 0 Z9 0 U1 7 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0038-6308 EI 1572-9672 J9 SPACE SCI REV JI Space Sci. Rev. PD NOV PY 2016 VL 203 IS 1-4 BP 245 EP 275 DI 10.1007/s11214-016-0284-x PG 31 WC Astronomy & Astrophysics SC Astronomy & Astrophysics GA EE2LO UT WOS:000389415800008 ER PT J AU Neakrase, LDV Balme, MR Esposito, F Kelling, T Klose, M Kok, JF Marticorena, B Merrison, J Patel, M Wurm, G AF Neakrase, L. D. V. Balme, M. R. Esposito, F. Kelling, T. Klose, M. Kok, J. F. Marticorena, B. Merrison, J. Patel, M. Wurm, G. TI Particle Lifting Processes in Dust Devils SO SPACE SCIENCE REVIEWS LA English DT Review DE Dust devils; Mars; Earth; Particle lifting; Aeolian processes ID THRESHOLD FRICTION VELOCITY; WIND EROSION THRESHOLD; MARS PATHFINDER; SHEAR VELOCITY; INTERANNUAL VARIABILITY; SALTATION BOMBARDMENT; CONVECTIVE VORTICES; TRANSPORT PROCESSES; SIZE DISTRIBUTIONS; FIELD OBSERVATIONS AB Particle lifting in dust devils on both Earth and Mars has been studied from many different perspectives, including how dust devils could influence the dust cycles of both planets. Here we review our current understanding of particle entrainment by dust devils by examining results from field observations on Earth and Mars, laboratory experiments (at terrestrial ambient and Mars-analog conditions), and analytical modeling. By combining insights obtained from these three methodologies, we provide a detailed overview on interactions between particle lifting processes due to mechanical, thermal, electrodynamical and pressure effects, and how these processes apply to dust devils on Earth and Mars. Experiments and observations have shown dust devils to be effective lifters of dust given the proper conditions on Earth and Mars. However, dust devil studies have yet to determine the individual roles of each of the component processes acting at any given time in dust devils. C1 [Neakrase, L. D. V.] New Mexico State Univ, Dept Astron, Las Cruces, NM 88003 USA. [Balme, M. R.; Patel, M.] Open Univ, Walton Hall, Milton Keynes MK7 6AA, Bucks, England. [Esposito, F.] INAF Osservatorio Astron Capodimonte, Naples, Italy. [Kelling, T.; Wurm, G.] Univ Duisburg Essen, Fac Phys, Duisburg, Germany. [Klose, M.] Univ Cologne, Inst Geophys & Meteorol, Cologne, Germany. [Klose, M.] USDA ARS Jornada Expt Range, Las Cruces, NM USA. [Kok, J. F.] Univ Calif Los Angeles, Dept Atmospher & Ocean Sci, Los Angeles, CA USA. [Marticorena, B.] Univ Paris, Lab Interuniv Syst Atmospher, Creteil, France. [Merrison, J.] Aarhus Univ, Inst Phys & Astron, Aarhus, Denmark. [Patel, M.] Rutherford Appleton Lab, Space Phys, Chilton, Oxon, England. RP Neakrase, LDV (reprint author), New Mexico State Univ, Dept Astron, Las Cruces, NM 88003 USA. EM lneakras@nmsu.edu RI Kok, Jasper/A-9698-2008; OI Kok, Jasper/0000-0003-0464-8325; Klose, Martina/0000-0001-8190-3700 FU National Science Foundation [AGS-1358621] FX This work was supported by grant National Science Foundation grant AGS-1358621 to J. K. NR 172 TC 1 Z9 1 U1 5 U2 5 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0038-6308 EI 1572-9672 J9 SPACE SCI REV JI Space Sci. Rev. PD NOV PY 2016 VL 203 IS 1-4 BP 347 EP 376 DI 10.1007/s11214-016-0296-6 PG 30 WC Astronomy & Astrophysics SC Astronomy & Astrophysics GA EE2LO UT WOS:000389415800011 ER PT J AU Klose, M Jemmett-Smith, BC Kahanpaa, H Kahre, M Knippertz, P Lemmon, MT Lewis, SR Lorenz, RD Neakrase, LDV Newman, C Patel, MR Reiss, D Spiga, A Whelley, PL AF Klose, Martina Jemmett-Smith, Bradley C. Kahanpaa, Henrik Kahre, Melinda Knippertz, Peter Lemmon, Mark T. Lewis, Stephen R. Lorenz, Ralph D. Neakrase, Lynn D. V. Newman, Claire Patel, Manish R. Reiss, Dennis Spiga, Aymeric Whelley, Patrick L. TI Dust Devil Sediment Transport: From Lab to Field to Global Impact SO SPACE SCIENCE REVIEWS LA English DT Review DE Dust devils; Dust emission; Lab experiments; Field measurements; Modeling; Dust environmental impact; Sediment transport; Earth; Mars; Planetary atmospheres ID LARGE-EDDY SIMULATION; PLANETARY BOUNDARY-LAYER; GENERAL-CIRCULATION MODEL; CONVECTIVE MIXED-LAYER; INTERANNUAL VARIABILITY; OPTICAL DEPTH; AUSTRALIAN LANDSCAPE; MARTIAN ATMOSPHERE; WILLY-WILLIES; SOUTHERN PERU AB The impact of dust aerosols on the climate and environment of Earth and Mars is complex and forms a major area of research. A difficulty arises in estimating the contribution of small-scale dust devils to the total dust aerosol. This difficulty is due to uncertainties in the amount of dust lifted by individual dust devils, the frequency of dust devil occurrence, and the lack of statistical generality of individual experiments and observations. In this paper, we review results of observational, laboratory, and modeling studies and provide an overview of dust devil dust transport on various spatio-temporal scales as obtained with the different research approaches. Methods used for the investigation of dust devils on Earth and Mars vary. For example, while the use of imagery for the investigation of dust devil occurrence frequency is common practice for Mars, this is less so the case for Earth. Modeling approaches for Earth and Mars are similar in that they are based on the same underlying theory, but they are applied in different ways. Insights into the benefits and limitations of each approach suggest potential future research focuses, which can further reduce the uncertainty associated with dust devil dust entrainment. The potential impacts of dust devils on the climates of Earth and Mars are discussed on the basis of the presented research results. C1 [Klose, Martina] Univ Cologne, Inst Geophys & Meteorol, Cologne, Germany. [Klose, Martina] USDA ARS Jornada Expt Range, Las Cruces, NM USA. [Jemmett-Smith, Bradley C.] Univ Leeds, Sch Earth & Environm, Inst Climate & Atmospher Sci, Leeds, W Yorkshire, England. [Kahanpaa, Henrik] Finnish Meteorol Inst, Helsinki, Finland. [Kahre, Melinda] NASA Ames Res Ctr, Moffett Field, CA USA. [Knippertz, Peter] Karlsruhe Inst Technol, Inst Meteorol & Climate Res, Karlsruhe, Germany. [Lemmon, Mark T.] Texas A&M Univ, Dept Atmospher Sci, College Stn, TX USA. [Lewis, Stephen R.; Patel, Manish R.] Open Univ, Dept Phys Sci, Milton Keynes, Bucks, England. [Lorenz, Ralph D.] Johns Hopkins Univ, Appl Phys Lab, Laurel, MD USA. [Neakrase, Lynn D. V.] New Mexico State Univ, Dept Astron, Las Cruces, NM USA. [Newman, Claire] Aeolis Res, Suite 205,600 North Rosemead Blvd, Pasadena, CA 91107 USA. [Reiss, Dennis] Westfal Wilhelms Univ, Inst Planetol, Munster, Germany. [Spiga, Aymeric] Univ Paris 06, Meteorol Dynam Lab, Paris, France. [Whelley, Patrick L.] NASA Goddard Space Flight Ctr, Greenbelt, MD USA. RP Klose, M (reprint author), Univ Cologne, Inst Geophys & Meteorol, Cologne, Germany.; Klose, M (reprint author), USDA ARS Jornada Expt Range, Las Cruces, NM USA. EM mklose@nmsu.edu; b.jemmett-smith@leeds.ac.uk; henrik.kahanpaa@fmi.fi; melinda.a.kahre@nasa.gov; peter.knippertz@kit.edu; lemmon@tamu.edu; stephen.lewis@open.ac.uk; Ralph.Lorenz@jhuapl.edu; lneakras@nmsu.edu; claire@aeolisresearch.com; manish.patel@open.ac.uk; dennis.reiss@uni-muenster.de; spiga@lmd.jussieu.fr; patrick.l.whelley@nasa.gov RI Spiga, Aymeric/O-4858-2014; OI Spiga, Aymeric/0000-0002-6776-6268; Reiss, Dennis/0000-0002-1836-596X; Klose, Martina/0000-0001-8190-3700; Lewis, Stephen/0000-0001-7237-6494 FU European Research Council [257543]; NASA [NNX12AI04G] FX We wish to thank Luca Montabone, one anonymous reviewer, and two editors for their careful review and valuable comments, and Bruce Cantor for his permission to reuse Fig. 4 of Cantor et al. (2006) in this paper. Bradley Jemmett-Smith and Peter Knippertz would like to acknowledge funding from the European Research Council Grant 257543 "Desert Storms". Ralph Lorenz acknowledges the support of NASA Mars Fundamental Research Program grant NNX12AI04G. Not least, we are grateful to the International Space Science Institute (ISSI), Bern, Switzerland, and to the conveners for organizing the workshop "Dust Devils on Mars and Earth" (www.issibern.ch/workshops/dustdevils/). NR 151 TC 4 Z9 4 U1 5 U2 5 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0038-6308 EI 1572-9672 J9 SPACE SCI REV JI Space Sci. Rev. PD NOV PY 2016 VL 203 IS 1-4 BP 377 EP 426 DI 10.1007/s11214-016-0261-4 PG 50 WC Astronomy & Astrophysics SC Astronomy & Astrophysics GA EE2LO UT WOS:000389415800012 ER PT J AU Clevenger, J Marasigan, K Liakos, V Sobolev, V Vellidis, G Holbrook, C Ozias-Akins, P AF Clevenger, Josh Marasigan, Kathleen Liakos, Vasileios Sobolev, Victor Vellidis, George Holbrook, Corley Ozias-Akins, Peggy TI RNA Sequencing of Contaminated Seeds Reveals the State of the Seed Permissive for Pre-Harvest Aflatoxin Contamination and Points to a Potential Susceptibility Factor SO TOXINS LA English DT Article DE Arachis hypogaea; Aspergillus; aflatoxin; RNA-seq; non-coding RNA; uHPLC ID ASPERGILLUS-FLAVUS INFECTION; ABSCISIC-ACID; DROUGHT TOLERANCE; PEANUT GENOTYPES; ARABIDOPSIS; GENE; GENOME; BIOSYNTHESIS; REGISTRATION; EXPRESSION AB Pre-harvest aflatoxin contamination (PAC) is a major problem facing peanut production worldwide. Produced by the ubiquitous soil fungus, Aspergillus flavus, aflatoxin is the most naturally occurring known carcinogen. The interaction between fungus and host resulting in PAC is complex, and breeding for PAC resistance has been slow. It has been shown that aflatoxin production can be induced by applying drought stress as peanut seeds mature. We have implemented an automated rainout shelter that controls temperature and moisture in the root and peg zone to induce aflatoxin production. Using polymerase chain reaction (PCR) and high performance liquid chromatography (HPLC), seeds meeting the following conditions were selected: infected with Aspergillus flavus and contaminated with aflatoxin; and not contaminated with aflatoxin. RNA sequencing analysis revealed groups of genes that describe the transcriptional state of contaminated vs. uncontaminated seed. These data suggest that fatty acid biosynthesis and abscisic acid (ABA) signaling are altered in contaminated seeds and point to a potential susceptibility factor, ABR1, as a repressor of ABA signaling that may play a role in permitting PAC. C1 [Clevenger, Josh; Marasigan, Kathleen; Ozias-Akins, Peggy] Univ Georgia, Dept Hort, Tifton, GA 31793 USA. [Clevenger, Josh; Marasigan, Kathleen; Ozias-Akins, Peggy] Univ Georgia, Inst Plant Breeding Genet & Genom, Tifton, GA 31793 USA. [Liakos, Vasileios; Vellidis, George] Univ Georgia, Dept Crop & Soil Sci, Tifton, GA 31793 USA. [Sobolev, Victor] USDA ARS, Natl Peanut Res Lab, Dawson, GA 39842 USA. [Holbrook, Corley] USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA USA. RP Ozias-Akins, P (reprint author), Univ Georgia, Dept Hort, Tifton, GA 31793 USA.; Ozias-Akins, P (reprint author), Univ Georgia, Inst Plant Breeding Genet & Genom, Tifton, GA 31793 USA. EM jclev@uga.edu; kath1290@uga.edu; vliakos@uga.edu; victor.sobolev@ars.usda.gov; yiorgos@uga.edu; corley.holbrook@ars.usda.gov; pozias@uga.edu FU Agriculture and Food Research Initiative of the USDA National Institute of Food and Agriculture [2012-85117-19435]; Feed the Future Innovation Lab for Collaborative Research on Peanut Productivity and Mycotoxin Control (Peanut and Mycotoxin Innovation Lab) - United States Agency for International Development (USAID) FX This project was funded by the Agriculture and Food Research Initiative competitive grant 2012-85117-19435 of the USDA National Institute of Food and Agriculture and the Feed the Future Innovation Lab for Collaborative Research on Peanut Productivity and Mycotoxin Control (Peanut and Mycotoxin Innovation Lab), supported by funding from the United States Agency for International Development (USAID) NR 57 TC 0 Z9 0 U1 3 U2 3 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2072-6651 J9 TOXINS JI Toxins PD NOV PY 2016 VL 8 IS 11 AR 317 DI 10.3390/toxins8110317 PG 18 WC Toxicology SC Toxicology GA EE1KW UT WOS:000389341400013 ER PT J AU Wolken, JM Mann, DH Grant, TA Lloyd, AH Rupp, TS Hollingsworth, TN AF Wolken, Jane M. Mann, Daniel H. Grant, Thomas A., III Lloyd, Andrea H. Rupp, T. Scott Hollingsworth, Teresa N. TI Climate-growth relationships along a black spruce toposequence in interior Alaska SO ARCTIC ANTARCTIC AND ALPINE RESEARCH LA English DT Article ID PICEA-MARIANA MILL.; WHITE SPRUCE; TREE-GROWTH; BOREAL FORESTS; TEMPERATURE SENSITIVITY; DROUGHT STRESS; BIOME SHIFT; RESPONSES; FIRE; COMMUNITIES AB Despite its wide geographic distribution and important role in boreal forest fire regimes, little is known about the climate-growth relationships of black spruce (Picea mariana [Mill.] B.S.P.). We used site-and tree-level analyses to evaluate the radial growth responses to climate of black spruce growing on a north-facing toposequence in interior Alaska for the period A.D. 1949-2010. At the site level, correlations between growth and climate were negative for temperature and positive for precipitation. The signs and strengths of these correlations varied seasonally and over time. These site-level differences probably arise from tree interactions with non-climatic factors that vary with topography and include active layer thickness, soil temperature, solar radiation, microsite, and tree architecture. We infer that black spruce suffers from drought stress during warm, dry summers and that the causes of this moisture stress relate to topography and the seasonality of drought. Tree-level analyses reveal that divergent inter-tree growth responses among individual trees at the same site also occur, with the lower slope positions having the greatest frequency of mixed responses. The overall complexity of black spruce's climate-growth relationships reflects the plastic growth strategy that enables this species to tolerate harsh, high-latitude conditions across a transcontinental range. C1 [Wolken, Jane M.; Rupp, T. Scott] Univ Alaska Fairbanks, Scenarios Network Alaska & Arctic Planning, POB 757245, Fairbanks, AK 99775 USA. [Mann, Daniel H.] Univ Alaska Fairbanks, Dept Geosci, Fairbanks, AK 99775 USA. [Grant, Thomas A., III] Western State Colorado Univ, Environm Management Program, Gunnison, CO 81231 USA. [Lloyd, Andrea H.] Middlebury Coll, Dept Biol, Middlebury, VT 05753 USA. [Hollingsworth, Teresa N.] Univ Alaska Fairbanks, Forest Serv, USDA, Pacific Northwest Res Stn,Boreal Ecol Cooperat Re, POB 756780, Fairbanks, AK 99775 USA. RP Wolken, JM (reprint author), Univ Alaska Fairbanks, Scenarios Network Alaska & Arctic Planning, POB 757245, Fairbanks, AK 99775 USA. EM jmwolken@alaska.edu FU National Science Foundation [ARC-0902169]; Scenarios Network for Alaska and Arctic Planning; Bonanza Creek LTER NSF award [DEB-1026415, DEB-0620579, DEB-0423442, DEB-0080609, DEB-9810217, DEB-9211769, DEB-8702629]; USDA Forest Service, Pacific Northwest Research Station [RJVA-PNW-01-JV-11261952-231]; Alaska Climate Science Center (U.S. Geological Survey [USGS]) [G10AC00588] FX We thank Tom Kurkowski for creating maps; Angelica Floyd, Matt Leonawicz, and Michael Lindgren for assistance with figures; Sergey Marchenko for providing soil temperature data; Glenn Juday for providing climate data and laboratory equipment; David Spencer for assistance with COFECHA; and Claire Hudson, Carson Baughman, and Pamela Groves for assistance with field work. Funding was provided by a National Science Foundation grant (ARC-0902169), the Scenarios Network for Alaska and Arctic Planning, the Bonanza Creek LTER NSF awards (DEB-1026415, DEB-0620579, DEB-0423442, DEB-0080609, DEB-9810217, DEB-9211769, DEB-8702629), the USDA Forest Service, Pacific Northwest Research Station (Cooperative Agreement number RJVA-PNW-01-JV-11261952-231), and the Alaska Climate Science Center (Cooperative Agreement number G10AC00588 from the U.S. Geological Survey [USGS]). The contents of this paper are solely the responsibility of the authors and do not necessarily represent the official views of the USGS. NR 61 TC 0 Z9 0 U1 6 U2 6 PU INST ARCTIC ALPINE RES PI BOULDER PA UNIV COLORADO, BOULDER, CO 80309 USA SN 1523-0430 EI 1938-4246 J9 ARCT ANTARCT ALP RES JI Arct. Antarct. Alp. Res. PD NOV PY 2016 VL 48 IS 4 BP 637 EP 652 DI 10.1657/AAAR0015-056 PG 16 WC Environmental Sciences; Geography, Physical SC Environmental Sciences & Ecology; Physical Geography GA EE1CB UT WOS:000389317200003 ER PT J AU Chendev, YG Hubbart, JA Terekhin, EA Lupo, AR Sauer, TJ Burras, CL AF Chendev, Yury G. Hubbart, Jason A. Terekhin, Edgar A. Lupo, Anthony R. Sauer, Tom J. Burras, C. Lee TI Recent Afforestation in the Iowa River and Vorskla River Basins: A Comparative Trends Analysis SO FORESTS LA English DT Article DE afforestation; climate change; Iowa River Basin; Vorskla River Basin; remote sensing ID EASTERN UNITED-STATES; SATELLITE IMAGERY; AERIAL-PHOTOGRAPHY; COVER CHANGES; FOREST COVER; TREE GROWTH; CARBON; CLIMATE; USA; PRECIPITATION AB Afforestation trends were compared between two continentally-distinct, yet similar ecoregions to characterize similarities or differences in forest advancement due to natural and anthropogenic forcings. Temporal changes in forest cover were analyzed using high resolution aerial and satellite photographs for Southeast Iowa, USA, and satellite photographs for the western Belgorod Oblast, Russia. An increase in forested area was shown to occur over a 44-year period from 1970-2014 in Iowa where afforestation was reflected by the aggregation of smaller forest units. In the Belgorod region the opposite occurred in that there was an increase in the number of smaller forested units. The rate of forest expansion into open grassland areas, previously used as haying lands and pastures, was 14 m decade(-1) and 8 m decade(-1) in Iowa and the Belgorod Oblast, respectively. Based on current trends, predicted times for complete forest coverage in the study areas was estimated to be 80 years in Iowa and 300 years in the Belgorod Oblast. In both the Iowa and Belgorod Oblast, there was an increase in annual precipitation at the end of the 20th and the beginning of the 21st centuries, thus providing a contributing mechanism to forest advancement in the study regions and implications for future management practices. C1 [Chendev, Yury G.; Terekhin, Edgar A.; Lupo, Anthony R.] Belgorod State Univ, Dept Nat Resource Management & Land Cadastre, Pobeda St 85, Belgorod 308015, Russia. [Hubbart, Jason A.] West Virginia Univ, Inst Water Secur & Sci, Davis Coll, Sch Agr, 3109 Agr Sci Bldg, Morgantown, WV 26506 USA. [Hubbart, Jason A.] West Virginia Univ, Inst Water Secur & Sci, Davis Coll, Sch Food & Nat Resources, 3109 Agr Sci Bldg, Morgantown, WV 26506 USA. [Lupo, Anthony R.] Univ Missouri, Dept Forestry, 302 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. [Lupo, Anthony R.] Univ Missouri, Dept Soil Environm & Atmospher Sci, 302 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. [Sauer, Tom J.] USDA, Natl Lab Agr & Environm, 1015 N Univ Blvd, Ames, IA 50011 USA. [Burras, C. Lee] Iowa State Univ, Dept Agron, 1126 Agron Hall, Ames, IA 50011 USA. RP Hubbart, JA (reprint author), West Virginia Univ, Inst Water Secur & Sci, Davis Coll, Sch Agr, 3109 Agr Sci Bldg, Morgantown, WV 26506 USA.; Hubbart, JA (reprint author), West Virginia Univ, Inst Water Secur & Sci, Davis Coll, Sch Food & Nat Resources, 3109 Agr Sci Bldg, Morgantown, WV 26506 USA. EM sciences@mail.ru; Jason.Hubbart@mail.wvu.edu; terekhin@bsu.edu.ru; LupoA@missouri.edu; Tom.Sauer@ars.usda.gov; lburras@iastate.edu RI Terekhin, Edgar/D-1356-2016 OI Terekhin, Edgar/0000-0002-5732-5039 FU Russian Science Foundation [14-17-00171]; Council for International Education/Fulbright Program FX This study was supported by the Russian Science Foundation, Project No. 14-17-00171 "Regional responses of environmental components on climate change varying periodicity: South forest-steppe of the Central Russian Upland", and the Council for International Education/Fulbright Program, as well as the anonymous reviewers who helped make this paper a stronger contribution. NR 65 TC 0 Z9 0 U1 5 U2 5 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD NOV PY 2016 VL 7 IS 11 AR 278 DI 10.3390/f7110278 PG 21 WC Forestry SC Forestry GA ED2KB UT WOS:000388672000029 ER PT J AU Daniels, DA Nix, KA Wadl, PA Vito, LM Wiggins, GJ Windham, MT Ownley, BH Lambdin, PL Grant, JE Merten, P Klingeman, WE Hadziabdic, D AF Daniels, Dixie A. Nix, Katheryne A. Wadl, Phillip A. Vito, Lisa M. Wiggins, Gregory J. Windham, Mark T. Ownley, Bonnie H. Lambdin, Paris L. Grant, Jerome E. Merten, Paul Klingeman, William E. Hadziabdic, Denita TI Thousand Cankers Disease Complex: A Forest Health Issue that Threatens Juglans Species across the US SO FORESTS LA English DT Review DE eastern black walnut; walnut twig beetle; fungal pathogen; Geosmithia morbida; Juglans nigra; Pterocarya spp.; Pityophthorus juglandis; insect vector; forest health ID WALNUT TWIG BEETLE; EASTERN UNITED-STATES; COLEOPTERA-CURCULIONIDAE SCOLYTINAE; BLACK-WALNUT; PITYOPHTHORUS-JUGLANDIS; GEOSMITHIA-MORBIDA; CAUSAL AGENT; GENETIC DIVERSITY; FUNGAL ENDOPHYTES; 1ST REPORT AB Thousand Cankers Disease (TCD) is a disease complex wherein the fungus (Geosmithia morbida) is vectored by the walnut twig beetle (WTB, Pityophthorus juglandis). The disease causes mortality primarily of eastern black walnut (Juglans nigra), although other walnut and wingnut (Pterocarya) species are also susceptible. Black walnut is native to the Eastern and Midwestern U.S. but is widely planted in western states. Total standing volume in both urban and forested settings is approximately 96 million cubic meters, and is valued at $539 billion. Although native to the Southwestern U.S., the range of WTB has expanded considerably. The spread of G. morbida coincides with that of WTB. TCD was introduced into Tennessee in 2010, and has spread to seven eastern states. Trees infected with TCD exhibit drought-like symptoms, making field detection difficult without molecular and/or morphological methods. The recently sequenced G. morbida genome will provide valuable research tools focused on understanding gene interactions between organisms involved in TCD and mechanisms of pathogenicity. With no chemical treatments available, quarantine and sanitation are preeminent options for slowing the spread of TCD, although biological control agents have been discovered. High levels of black walnut mortality due to TCD will have far-reaching implications for both eastern and western states. C1 [Daniels, Dixie A.] Oregon State Univ, Dept Forest Engn Resources & Management, 280 Peavy Hall, Corvallis, OR 97333 USA. [Nix, Katheryne A.; Vito, Lisa M.; Wiggins, Gregory J.; Windham, Mark T.; Ownley, Bonnie H.; Lambdin, Paris L.; Grant, Jerome E.; Hadziabdic, Denita] Univ Tennessee, Dept Entomol & Plant Pathol, 2505 EJ Chapman Dr,370 Plant Biotechnol Bldg, Knoxville, TN 37996 USA. [Wadl, Phillip A.] ARS, USDA, US Vegetable Lab, 2700 Savannah Highway, Charleston, SC 2700 USA. [Merten, Paul] US Forest Serv, USDA, Forest Hlth Protect, 200 WT Weaver Blvd, Asheville, NC 28804 USA. [Klingeman, William E.] Univ Tennessee, Dept Plant Sci, 2431 Joe Johnson Dr,252 Ellington Plant Sci Bldg, Knoxville, TN 37996 USA. RP Hadziabdic, D (reprint author), Univ Tennessee, Dept Entomol & Plant Pathol, 2505 EJ Chapman Dr,370 Plant Biotechnol Bldg, Knoxville, TN 37996 USA. EM dixie.daniels@oregonstate.edu; kavery3@utk.edu; PHILLIP.WADL@ars.usda.gov; lvito@utk.edu; wiggybug@utk.edu; mwindham@utk.edu; bownley@utk.edu; plambdin@utk.edu; jgrant@utk.edu; pmerten@fs.fed.us; wklingem@utk.edu; dhadziab@utk.edu FU United States Forest Service [13-DG-11083150-033]; United States Forest Service-Special Technology Development Program [13-DG-11083150-039]; USDA National Institute of Food and Agriculture; Hatch project [1009630]; United States Department of Agriculture [2015-70006-24159] FX The authors thank United States Forest Service (Grant number 13-DG-11083150-033) and United States Forest Service-Special Technology Development Program (Grant numbers 13-DG-11083150-039) for partial financial support. This work is also supported, in part, by the USDA National Institute of Food and Agriculture, Hatch project 1009630 and the United States Department of Agriculture (Grant number 2015-70006-24159). Special thanks to research assistants and associates, as well as graduate and undergraduate students for their support to ongoing research efforts, enthusiasm, and for furthering knowledge regarding TCD. The authors additionally thank independent reviewers for their comments and suggestions for manuscript improvement. Thanks also to Dr. Jim Kiser for guidance and suggestions. Finally, the authors thank Lisa M. Vito, author on this paper who passed away before publishing. Her contributions to plant pathology will be remembered. NR 89 TC 0 Z9 0 U1 11 U2 11 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD NOV PY 2016 VL 7 IS 11 AR 260 DI 10.3390/f7110260 PG 15 WC Forestry SC Forestry GA ED2KB UT WOS:000388672000011 ER PT J AU Halofsky, JE Peterson, DL Metlen, KL Myer, MG Sample, VA AF Halofsky, Jessica E. Peterson, David L. Metlen, Kerry L. Myer, M. Gwyneth Sample, V. Alaric TI Developing and Implementing Climate Change Adaptation Options in Forest Ecosystems: A Case Study in Southwestern Oregon, USA SO FORESTS LA English DT Article DE adaptation; climate change; resource management; vegetation; vulnerability assessment; southwestern Oregon; Klamath-Siskiyou ecoregion ID WESTERN UNITED-STATES; NATURAL-RESOURCE MANAGEMENT; NORTHERN CALIFORNIA; SISKIYOU MOUNTAINS; PACIFIC-NORTHWEST; SEVERITY FIRE; CONIFER REGENERATION; KLAMATH MOUNTAINS; WILDFIRE; DYNAMICS AB Climate change will likely have significant effects on forest ecosystems worldwide. In Mediterranean regions, such as that in southwestern Oregon, USA, changes will likely be driven mainly by wildfire and drought. To minimize the negative effects of climate change, resource managers require tools and information to assess climate change vulnerabilities and to develop and implement adaptation actions. We developed an approach to facilitate development and implementation of climate change adaptation options in forest management. This approach, applied in a southwestern Oregon study region, involved establishment of a science-manager partnership, a science-based assessment of forest and woodland vulnerabilities to climate change, climate change education in multiple formats, hands-on development of adaptation options, and application of tools to incorporate climate change in planned projects. Through this approach, we improved local manager understanding of the potential effects of climate change in southwestern Oregon, and enabled evaluation of proposed management activities in the context of climatic stressors. Engaging managers throughout the project increased ownership of the process and outcomes, as well as the applicability of the adaptation options toe-ground actions. Science-management partnerships can effectively inco on-thrporate evolving science, regardless of the socio-political environment, and facilitate timely progress in adaptation to climate change. C1 [Halofsky, Jessica E.] Univ Washington, Sch Environm & Forest Sci, Box 352100, Seattle, WA 98195 USA. [Peterson, David L.] US Forest Serv, Pacific Northwest Res Stn, 400 N 34th St, Seattle, WA 98103 USA. [Metlen, Kerry L.] Nature Conservancy, 33 North Cent Ave,Suite 405, Medford, OR 97501 USA. [Myer, M. Gwyneth] Southern Oregon Forest Restorat Collaborat, 13401 Highway 66, Ashland, OR 97520 USA. [Sample, V. Alaric] Pinchot Inst Conservat, 1400 16th St Northwest,Suite 350, Washington, DC 20036 USA. RP Halofsky, JE (reprint author), Univ Washington, Sch Environm & Forest Sci, Box 352100, Seattle, WA 98195 USA. EM jhalo@uw.edu; peterson@fs.fed.us; kmetlen@tnc.org; gwynmyer@gmail.com; alsample@pinchot.org FU U.S. Department of the Interior, North Pacific Landscape Conservation Cooperative; Wildlife Conservation Society; U.S. Forest Service Pacific Northwest Research Station FX This project was funded by the U.S. Department of the Interior, North Pacific Landscape Conservation Cooperative, the Wildlife Conservation Society, and the U.S. Forest Service Pacific Northwest Research Station. We thank the many engaged participants in the webinars and workshops, and appreciate the thoughtful review by Darren Borgias. The Table Rocks climate adaptation demonstration project was a success because of highly engaged partners, including Lomakatsi Restoration Project, Wildlife Conservation Society, Klamath Bird Observatory, TNC, Medford District Bureau of Land Management, U.S. Fish and Wildlife Service, and Natural Resources Conservation Service. NR 106 TC 0 Z9 0 U1 12 U2 12 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD NOV PY 2016 VL 7 IS 11 AR 268 DI 10.3390/f7110268 PG 18 WC Forestry SC Forestry GA ED2KB UT WOS:000388672000019 ER PT J AU Huang, CY Gonzalez, G Hendrix, PF AF Huang, Ching -Yu Gonzalez, Grizelle Hendrix, Paul F. TI Resource Utilization by Native and Invasive Earthworms and Their Effects on Soil Carbon and Nitrogen Dynamics in Puerto Rican Soils SO FORESTS LA English DT Article DE carbon and nitrogen mineralization; invasive earthworms; Luquillo mountains; microbial respiration; Puerto Rico; stable isotope; tropics ID MICROBIAL BIOMASS; PONTOSCOLEX-CORETHRURUS; EXOTIC EARTHWORMS; ENDOGEIC EARTHWORMS; TEMPERATE FORESTS; NATURAL-ABUNDANCE; EXTRACTION METHOD; WET FOREST; N-15; OLIGOCHAETA AB Resource utilization by earthworms affects soil C and N dynamics and further colonization of invasive earthworms. By applying C-13-labeled Tabebuia heterophylla leaves and N-15-labeled Andropogon glomeratus grass, we investigated resource utilization by three earthworm species (invasive endogeic Pontoscolex corethrurus, native anecic Estherella sp., and native endogeic Onychochaeta borincana) and their effects on soil C and N dynamics in Puerto Rican soils in a 22-day laboratory experiment. Changes of C-13/C and N-15/N in soils, earthworms, and microbial populations were analyzed to evaluate resource utilization by earthworms and their influences on C and N dynamics. Estherella spp. utilized the C-13-labeled litter; however, its utilization on the C-13-labeled litter reduced when cultivated with P. corethrurus and O. borincana. Both P. corethrurus and O. borincana utilized the C-13-labeled litter and C-15-labeled grass roots and root exudates. Pontoscolex corethrurus facilitated soil respiration by stimulating C-13-labeled microbial activity; however, this effect was suppressed possibly due to the changes in the microbial activities or community when coexisting with O. borincana. Increased soil N mineralization by individual Estherella spp. and O. borincana was reduced in the mixed-species treatments. The rapid population growth of P. corethrurus may increase competition pressure on food resources on the local earthworm community. The relevance of resource availability to the population growth of P. corethrurus and its significance as an invasive species is a topic in need of future research. C1 [Huang, Ching -Yu; Hendrix, Paul F.] Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. [Huang, Ching -Yu] Univ North Georgia, Dept Biol, Dahlonega, GA 30597 USA. [Gonzalez, Grizelle] US Forest Serv, USDA, Int Inst Trop Forestry, 1201 Ceiba St, Rio Piedras, PR 00926 USA. RP Huang, CY (reprint author), Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA.; Huang, CY (reprint author), Univ North Georgia, Dept Biol, Dahlonega, GA 30597 USA. EM ching-yu.huang@ung.edu; ggonzalez@fs.fed.us; hendrixp@uga.edu FU National Science Foundation [0236276]; Graduate Student Research Prize, Center for Biodiversity and Ecosystem Processes, University of Georgia; NSF [DEB-0218039]; USDA Forest Service-IITF as part of the LTER program in the Luquillo Experimental Forest FX We thank the staff at the Sabana Field Research Station and the International Institute of Tropical Forestry (IITF) for their help in the lab and in the field. We also thank David Kissel, the Soil Plant and Water Laboratory and the University of Georgia, for helping us with the soil transportation and sterilization process. This research was supported by the National Science Foundation grant number 0236276 to the University of Georgia Research Foundation, Inc.; the Graduate Student Research Prize, Center for Biodiversity and Ecosystem Processes, University of Georgia; grant DEB-0218039 from the NSF to the Institute of Tropical Ecosystem Studies, University of Puerto Rico, and the USDA Forest Service-IITF as part of the LTER program in the Luquillo Experimental Forest. All research at the USDA Forest Service International Institute of Tropical Forestry was performed in collaboration with the University of Puerto Rico. We also thank Ariel E. Lugo, Frank Wadsworth, and two anonymous reviewers, for their comments and suggestions on an earlier version of this manuscript. NR 53 TC 0 Z9 0 U1 11 U2 11 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD NOV PY 2016 VL 7 IS 11 AR 277 DI 10.3390/f7110277 PG 18 WC Forestry SC Forestry GA ED2KB UT WOS:000388672000028 ER PT J AU Lodge, DJ Winter, D Gonzalez, G Clum, N AF Lodge, D. Jean Winter, Dirk Gonzalez, Grizelle Clum, Naomi TI Effects of Hurricane-Felled Tree Trunks on Soil Carbon, Nitrogen, Microbial Biomass, and Root Length in a Wet Tropical Forest SO FORESTS LA English DT Article DE coarse woody debris; nitrogen cycling; soil carbon; soil nitrogen; tree roots; bark beetle frass; fine root proliferation; nutrient hotspots ID COARSE WOODY DEBRIS; ORGANIC-MATTER DYNAMICS; PUERTO-RICO; SUBTROPICAL WET; NUTRIENT DYNAMICS; CLIMATE-CHANGE; RAIN-FOREST; LITTER DECOMPOSITION; CYRILLA-RACEMIFLORA; INGROWTH CORES AB Decaying coarse woody debris can affect the underlying soil either by augmenting nutrients that can be exploited by tree roots, or by diminishing nutrient availability through stimulation of microbial nutrient immobilization. We analyzed C, N, microbial biomass C and root length in closely paired soil samples taken under versus 20-50 cm away from large trunks of two species felled by Hugo (1989) and Georges (1998) three times during wet and dry seasons over the two years following the study conducted by Georges. Soil microbial biomass, % C and % N were significantly higher under than away from logs felled by both hurricanes (i.e., 1989 and 1998), at all sampling times and at both depths (0-10 and 10-20 cm). Frass from wood boring beetles may contribute to early effects. Root length was greater away from logs during the dry season, and under logs in the wet season. Root length was correlated with microbial biomass C, soil N and soil moisture (R = 0.36, 0.18, and 0.27, respectively; all p values < 0.05). Microbial biomass C varied significantly among seasons but differences between positions (under vs. away) were only suggestive. Microbial C was correlated with soil N (R = 0.35). Surface soil on the upslope side of the logs had significantly more N and microbial biomass, likely from accumulation of leaf litter above the logs on steep slopes. We conclude that decaying wood can provide ephemeral resources that are exploited by tree roots during some seasons. C1 [Lodge, D. Jean] US Forest Serv, USDA, Northern Res Stn, Luquillo, PR 00773 USA. [Winter, Dirk] Columbia Univ, Dept Psychiat, 180 Ft Washington Ave, New York, NY 10032 USA. [Gonzalez, Grizelle] US Forest Serv, USDA, Int Inst Trop Forestry, Jardin Bot Sur, 1201 Ceiba St, Rio Piedras, PR 00926 USA. [Clum, Naomi] Carmel Valley Middle Sch, 3800 Mykonos Lane, San Diego, CA 92007 USA. RP Lodge, DJ (reprint author), US Forest Serv, USDA, Northern Res Stn, Luquillo, PR 00773 USA. EM dlodge@fs.fed.us; dcw4@caa.columbia.edu; ggonzalez@fs.fed.us; naomi.clum@sduhsd.net OI Gonzalez, Grizelle /0000-0003-3007-5540 FU National Science Foundation [DEB-0218039, 1239764]; United States Department of Agriculture, Forest Service, International Institute of Tropical Forestry as part of the Long-Term Ecological Research Program in the Luquillo Experimental Forest; Luquillo LCZO grant [EAR-1331841] FX This research was performed under grants DEB-0218039 and 1239764 from the National Science Foundation to the Institute of Tropical Ecosystem Studies, University of Puerto Rico, and the United States Department of Agriculture, Forest Service, International Institute of Tropical Forestry as part of the Long-Term Ecological Research Program in the Luquillo Experimental Forest. Additional support for G. Gonzalez was provided by the Luquillo LCZO grant (EAR-1331841). We are thankful to Maria M. Rivera, Mary Jeane Sanchez, Maysaa Ittayem, Edwin Lopez, Carmen Marrero, Marybelis Santiago and other staff at the International Institute of Tropical Forestry-Soil Laboratory who helped process soil samples; and Xiaoming Zou for advice on measuring soil microbial biomass. We thank the statistician for the United States Department of Agriculture, Forest Service, Northern Research Station, John Stanovick, for statistical analyses and review, and Walter Shortle and Tana Wood for pre-reviews that improved the manuscript. Additional support was provided by the University of Puerto Rico, Rio Piedras. NR 72 TC 0 Z9 0 U1 14 U2 14 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD NOV PY 2016 VL 7 IS 11 AR 264 DI 10.3390/f7110264 PG 19 WC Forestry SC Forestry GA ED2KB UT WOS:000388672000015 ER PT J AU Munck, IA Luther, T Wyka, S Keirstead, D McCracken, K Ostrofsky, W Searles, W Lombard, K Weimer, J Allen, B AF Munck, Isabel A. Luther, Thomas Wyka, Stephen Keirstead, Donald McCracken, Kimberly Ostrofsky, William Searles, Wayne Lombard, Kyle Weimer, Jennifer Allen, Bruce TI Soil and Stocking Effects on Caliciopsis Canker of Pinus strobus L. SO FORESTS LA English DT Article DE forest health monitoring; eastern white pine; tree density; tree disease ID EASTERN WHITE-PINE; ASSOCIATION; DISEASE; WATER AB Soil and stand density were found to be promising predictive variables associated with damage by the emerging disease of eastern white pine, Caliciopsis canker, in a 2014 survey with randomly selected eastern white pine (Pinus strobus L.) stands. The objective of this study was to further investigate the relationship between soil and stocking in eastern white pine forests of New England by stratifying sampling across soils and measuring stand density more systematically. A total of 62 eastern white pine stands were sampled during 2015-2016. Stands were stratified across soil groups and several prism plots were established at each site to measure stand density and determine stocking. Caliciopsis canker incidence in mature trees was greater in sites with drier or shallow soils compared to sites with loamy soils and in adequately stocked stands compared to understocked stands (p < 0.0001). Caliciopsis canker signs and symptoms were observed in all size classes. Live crown ratio, a measure of forest health, decreased with increasing Caliciopsis canker symptom severity. The fungal pathogen, Caliciopsis pinea Peck, was successfully isolated from cankers on trees growing in each soil group. Forest managers will need to consider damage caused by Caliciopsis canker related to stand factors such as soil and stocking when regenerating white pine stands. C1 [Munck, Isabel A.; Luther, Thomas; Wyka, Stephen] US Forest Serv, Northeastern Area State & Private Forestry, USDA, Durham, NH 03861 USA. [Keirstead, Donald; McCracken, Kimberly] NRCS, USDA, Dover, NH 03861 USA. [Ostrofsky, William; Searles, Wayne] Maine Forest Serv, Maine Dept Agr Conservat & Forestry, Augusta, MA 04692 USA. [Lombard, Kyle; Weimer, Jennifer; Allen, Bruce] New Hampshire Dept Resources & Econ Dev, New Hampshire Div Forests & Lands, Concord, NH 03861 USA. RP Munck, IA (reprint author), US Forest Serv, Northeastern Area State & Private Forestry, USDA, Durham, NH 03861 USA. EM imunck@fs.fed.us; imunck@fs.fed.us; stephenwyka@gmail.com; donald.keirstead@nh.usda.gov; kimberly.mccracken@nh.usda.gov; bill.ostrofsky@maine.gov; Wayne.Searles@maine.gov; kyle.lombard@dred.state.nh.us; Jennifer.Weimer@dred.nh.gov; Bruce.Allen@dred.nh.gov OI Munck, Isabel/0000-0001-6889-9196 FU U.S. Department of Agriculture (USDA) Forest Service (USFS) [14-DG-11420004-155, 14-DG-11420004-161, 14-DG-1142004-229] FX This work was partially funded by the U.S. Department of Agriculture (USDA) Forest Service (USFS) grants # 14-DG-11420004-155, 14-DG-11420004-161, and 14-DG-1142004-229. We are grateful to two anonymous reviewers and editors for the thoughtful suggestions that have improved this work. We would like to thank JacobMavrogeorge from the USFSPathway Program for field assistance, Michelle Cram, Elizabeth Burril, Michael Bohne, and Kevin Dodds from USFS, Lyndsay Hodgman from the USDA Natural Resources Conservation Service (NRCS), and Kara Constanza, William Livingston, and Shawn Fraver from the University of Maine for technical assistance and support. Lastly, we appreciate wisdom and guidance provided by William Leak, USFS. USDA is an equal opportunity provider and employer. NR 21 TC 0 Z9 0 U1 1 U2 1 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD NOV PY 2016 VL 7 IS 11 AR 269 DI 10.3390/f7110269 PG 10 WC Forestry SC Forestry GA ED2KB UT WOS:000388672000020 ER PT J AU Wolfe, MD Kulakow, P Rabbi, IY Jannink, JL AF Wolfe, Marnin D. Kulakow, Peter Rabbi, Ismail Y. Jannink, Jean-Luc TI Marker-Based Estimates Reveal Significant Nonadditive Effects in Clonally Propagated Cassava (Manihot esculenta): Implications for the Prediction of Total Genetic Value and the Selection of Varieties SO G3-GENES GENOMES GENETICS LA English DT Article DE Genomic selection; Nonadditive effects; Cassava; GenPred; Shared Data Resources ID GENOMIC SELECTION; DIALLEL ANALYSIS; MISSING HERITABILITY; QUANTITATIVE TRAITS; SUBHUMID CONDITIONS; RELEVANT TRAITS; BREEDING VALUES; DAIRY-CATTLE; EPISTASIS; RESISTANCE AB In clonally propagated crops, nonadditive genetic effects can be effectively exploited by the identification of superior genetic individuals as varieties. Cassava (Manihot esculenta Crantz) is a clonally propagated staple food crop that feeds hundreds of millions. We quantified the amount and nature of nonadditive genetic variation for three key traits in a breeding population of cassava from sub-Saharan Africa using additive and nonadditive genome-wide marker-based relationship matrices. We then assessed the accuracy of genomic prediction for total (additive plus nonadditive) genetic value. We confirmed previous findings based on diallel crosses that nonadditive genetic variation is significant for key cassava traits. Specifically, we found that dominance is particularly important for root yield and epistasis contributes strongly to variation in cassava mosaic disease (CMD) resistance. Further, we showed that total genetic value predicted observed phenotypes more accurately than additive only models for root yield but not for dry matter content, which is mostly additive or for CMD resistance, which has high narrow-sense heritability. We address the implication of these results for cassava breeding and put our work in the context of previous results in cassava, and other plant and animal species. C1 [Wolfe, Marnin D.; Jannink, Jean-Luc] Cornell Univ, Dept Plant Breeding & Genet, 417 Bradfield Hall,306 Tower Rd, Ithaca, NY 14853 USA. [Jannink, Jean-Luc] USDA ARS, RW Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Kulakow, Peter; Rabbi, Ismail Y.] IITA, Ibadan, Oyo, Nigeria. RP Wolfe, MD (reprint author), Cornell Univ, Dept Plant Breeding & Genet, 417 Bradfield Hall,306 Tower Rd, Ithaca, NY 14853 USA. EM wolfemd@gmail.com FU UKaid [1048542]; Consultative Group for International Agricultural Research (CGIAR) Research Program on Roots, Tubers and Bananas FX We give special thanks to A.G.O. Dixon for his development of many of the breeding lines and historical data we analyzed. We also thank A. I. Smith and technical teams at the International Institute of Tropical Agriculture for collection of phenotypic data, and A. Agbona and P. Peteti for data curation. We acknowledge the Bill & Melinda Gates Foundation and UKaid (grant 1048542; http://www.gatesfoundation.org) and support from the Consultative Group for International Agricultural Research (CGIAR) Research Program on Roots, Tubers and Bananas (http://www.rtb.cgiar.org). NR 75 TC 0 Z9 0 U1 3 U2 3 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD NOV PY 2016 VL 6 IS 11 BP 3497 EP 3506 DI 10.1534/g3.116.033332 PG 10 WC Genetics & Heredity SC Genetics & Heredity GA ED2LY UT WOS:000388678000006 ER PT J AU Zhou, XF Peris, D Kominek, J Kurtzman, CP Hittinger, CT Rokas, A AF Zhou, Xiaofan Peris, David Kominek, Jacek Kurtzman, Cletus P. Hittinger, Chris Todd Rokas, Antonis TI In Silico Whole Genome Sequencer and Analyzer (iWGS): a Computational Pipeline to Guide the Design and Analysis of de novo Genome Sequencing Studies SO G3-GENES GENOMES GENETICS LA English DT Article DE genome sequencing; high-throughput sequencing; de novo assembly; experimental design; simulation; nonmodel organism ID MICROBIAL GENOMES; SACCHAROMYCES-CEREVISIAE; MANAGEMENT-SYSTEM; MAMMALIAN GENOMES; READS SIMULATOR; ASSEMBLIES; VALIDATION; ALGORITHMS; ADAPTATION; DROSOPHILA AB The availability of genomes across the tree of life is highly biased toward vertebrates, pathogens, human disease models, and organisms with relatively small and simple genomes. Recent progress in genomics has enabled the de novo decoding of the genome of virtually any organism, greatly expanding its potential for understanding the biology and evolution of the full spectrum of biodiversity. The increasing diversity of sequencing technologies, assays, and de novo assembly algorithms have augmented the complexity of de novo genome sequencing projects in nonmodel organisms. To reduce the costs and challenges in de novo genome sequencing projects and streamline their experimental design and analysis, we developed iWGS (in silico Whole Genome Sequencer and Analyzer), an automated pipeline for guiding the choice of appropriate sequencing strategy and assembly protocols. iWGS seamlessly integrates the four key steps of a de novo genome sequencing project: data generation (through simulation), data quality control, de novo assembly, and assembly evaluation and validation. The last three steps can also be applied to the analysis of real data. iWGS is designed to enable the user to have great flexibility in testing the range of experimental designs available for genome sequencing projects, and supports all major sequencing technologies and popular assembly tools. Three case studies illustrate how iWGS can guide the design of de novo genome sequencing projects, and evaluate the performance of a wide variety of user-specified sequencing strategies and assembly protocols on genomes of differing architectures. iWGS, along with a detailed documentation, is freely available at https://github.com/zhouxiaofan1983/iWGS. C1 [Zhou, Xiaofan; Rokas, Antonis] Vanderbilt Univ, Dept Biol Sci, 221 Kirkland Hall, Nashville, TN 37235 USA. [Peris, David; Kominek, Jacek; Hittinger, Chris Todd] Univ Wisconsin, Great Lakes Bioenergy Res Ctr, Wisconsin Energy Inst,JF Crow Inst Study Evolut, Lab Genet,Genome Ctr Wisconsin,Dept Energy, Madison, WI 53706 USA. [Kurtzman, Cletus P.] ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Hittinger, CT (reprint author), Univ Wisconsin, Great Lakes Bioenergy Res Ctr,Wisconsin Energy In, Genome Ctr Wisconsin,JF Crow Inst Study Evolut, Lab Genet,DOE,Genet Biotechnol Ctr 4340 4360, 425-G Henry Mall, Madison, WI 53706 USA.; Rokas, A (reprint author), Vanderbilt Univ, Dept Biol Sci, VU Stn B 351634, Nashville, TN 37235 USA. EM cthittinger@wisc.edu; antonis.rokas@vanderbilt.edu FU Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231]; National Science Foundation [DEB-1442113, DEB-1253634, DEB-1442148]; DOE Great Lakes Bioenergy Research Center (DOE Office of Science) [BER DE-FC02-07ER64494]; U.S. Department of Agriculture (USDA) National Institute of Food and Agriculture [1003258]; National Institutes of Health [NIAID AI105619]; Alexander von Humboldt Foundation; Pew Charitable Trusts FX We thank Branden Timm for technical support on the WEI cluster. We thank Francis Martin for providing access to unpublished genome data for the C. geophilum genome produced by the U.S. Department of Energy Joint Genome Institute, a Department of Energy (DOE) Office of Science User Facility, supported by the Office of Science of the U.S. Department of Energy under contract no. DE-AC02-05CH11231. This work was conducted in part using the resources of the Advanced Computing Center for Research and Education at Vanderbilt University and the Wisconsin Energy Institute (WEI) cluster at the University of Wisconsin-Madison. This work was funded by the National Science Foundation (DEB-1442113 to A.R.; DEB-1253634 and DEB-1442148 to C.T.H.), in part by the DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DE-FC02-07ER64494 to C.T.H.), the U.S. Department of Agriculture (USDA) National Institute of Food and Agriculture (Hatch Project 1003258 to C.T.H.), and the National Institutes of Health (NIAID AI105619 to A.R.). C.T.H. is an Alfred Toepfer Faculty Fellow, supported by the Alexander von Humboldt Foundation. C.T.H. is a Pew Scholar in the Biomedical Sciences, supported by the Pew Charitable Trusts. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer. NR 71 TC 0 Z9 0 U1 3 U2 3 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD NOV PY 2016 VL 6 IS 11 BP 3655 EP 3662 DI 10.1534/g3.116.034249 PG 8 WC Genetics & Heredity SC Genetics & Heredity GA ED2LY UT WOS:000388678000022 ER PT J AU Cha, DH Mieles, AE Lahuatte, PF Cahuana, A Lincango, MP Causton, CE Tebbich, S Cimadom, A Teale, SA AF Cha, Dong H. Mieles, Alejandro E. Lahuatte, Paola F. Cahuana, Andrea Lincango, Marie Piedad Causton, Charlotte E. Tebbich, Sabine Cimadom, Arno Teale, Stephen A. TI Identification and Optimization of Microbial Attractants for Philornis downsi, an Invasive Fly Parasitic on Galapagos Birds SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Philornis downsi; Attractant; Invasive; Avian parasite; Yeast; Galapagos ID RHAGOLETIS-POMONELLA FLIES; SUZUKII DIPTERA DROSOPHILIDAE; SPOTTED WING DROSOPHILA; SOUTHERN UNITED-STATES; HOST FRUIT VOLATILES; ACETIC-ACID; FERMENTATION VOLATILES; DARWINS FINCHES; CHEMICAL LURE; MUSCIDAE AB We investigated the role of olfactory cues from actively fermenting yeast (Saccharomyces cerevisiae) in attraction of adult Philornis downsi and identified two synergistically attractive yeast volatiles. Larvae of this invasive fly parasitize the hatchlings of passerines and threaten the Galapagos avifauna. Gas chromatography coupled with electroantennographic detection (GC-EAD), coupled gas chromatography-mass spectrometry (GC-MS), and field trapping experiments were used to identify volatile compounds from a yeast-sugar solution. EAD responses were consistently elicited by 14 yeast volatiles. In a series of field trapping experiments, a mixture of the 14 EAD-active compounds was similarly attractive to P. downsi when compared to the yeast-sugar solution, and we found that acetic acid and ethanol were essential for attraction. A mixture of 0.03 % acetic acid and 3 % ethanol was as attractive as the 14-component blend, but was not as attractive as the yeast-sugar solution. Philornis downsi showed positive and negative dose-responses to acetic acid in the ranges of 0.01 similar to 0.3 % and 0.3 similar to 9 %, respectively. Further optimization showed that the mixture of 1% acetic acid and 3% ethanol was as attractive as the yeast-sugar solution. Both mixtures of acetic acid and ethanol were more selective than the yeast-sugar solution in terms of non-target moths and Polistes versicolor wasps captured. These results indicate that acetic acid and ethanol produced by yeasts are crucial for P. downsi attraction to fermented materials on which they feed as adults and can be used to manage this invasive fly in Galapagos. C1 [Cha, Dong H.; Mieles, Alejandro E.; Teale, Stephen A.] SUNY Coll Environm Sci & Forestry, Syracuse, NY 13210 USA. [Cha, Dong H.] ARS, USDA, US Pacific Basin Agr Res Lab, 64 Nowelo St, Hilo, HI USA. [Lahuatte, Paola F.; Cahuana, Andrea; Lincango, Marie Piedad; Causton, Charlotte E.] Charles Darwin Fdn Galapagos Isl, Santa Cruz Isl, Galapagos Islan, Ecuador. [Lincango, Marie Piedad] Univ Cent Ecuador, Fac Ciencias Agr, Quito, Ecuador. [Tebbich, Sabine; Cimadom, Arno] Univ Vienna, Dept Behav Biol, A-1090 Vienna, Austria. RP Teale, SA (reprint author), SUNY Coll Environm Sci & Forestry, Syracuse, NY 13210 USA. EM sateale@esf.edu FU Galapagos National Park Directorate [PC-10-15, 18-16]; USDA-APHIS; Galapagos Conservancy; International Community Foundation (Leona M. and Harry B. Helmsley Charitable Trust) FX We thank Patricio Macas, Celina Leuba, Wilson Iniguez, Landry Vasquez, and Courtney Pike for assistance in the field, Elizabeth Semler for maintaining live P. downsi in the laboratory, also James Gibbs, Stephen Blake, Charlie Blake, and George Heimpel for hand carrying P. downsi to the U.S. Permission to conduct this study was granted by the Galapagos National Park Directorate (Project: PC-10-15 & 18-16: Control of the Invasive Parasite, Philornis downsi and its Impact on Biodiversity). Permits to import P. downsi to the U.S. for laboratory work were granted by USDA-APHIS. This work was supported by funding from the Galapagos Conservancy and the International Community Foundation (with a grant awarded by The Leona M. and Harry B. Helmsley Charitable Trust) to the Charles Darwin Foundation and to the State University of New York, College of Environmental Science and Forestry. We thank two anonymous referees for critical comments on an earlier version of the manuscript. This is contribution number 2142 of the Charles Darwin Foundation for the Galapagos Islands. NR 40 TC 0 Z9 0 U1 4 U2 4 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 EI 1573-1561 J9 J CHEM ECOL JI J. Chem. Ecol. PD NOV PY 2016 VL 42 IS 11 BP 1101 EP 1111 DI 10.1007/s10886-016-0780-1 PG 11 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA ED4HJ UT WOS:000388808300001 PM 27744622 ER PT J AU Fleischman, EF Kowalski, RJ Morris, CF Nguyen, T Li, C Ganjyal, G Ross, CF AF Fleischman, Emily F. Kowalski, Ryan J. Morris, Craig F. Nguyen, Thuy Li, Chongjun Ganjyal, Girish Ross, Carolyn F. TI Physical, Textural, and Antioxidant Properties of Extruded Waxy Wheat Flour Snack Supplemented with Several Varieties of Bran SO JOURNAL OF FOOD SCIENCE LA English DT Article DE antioxidant; extrusion; texture; waxy-pen; wheat bran ID DIETARY FIBER; FUNCTIONAL-PROPERTIES; EXTRUSION CONDITIONS; BREAKFAST CEREAL; BY-PRODUCTS; STARCH; QUALITY; CORN; CAPACITY; GELATINIZATION AB Wheat represents a ubiquitous commodity and although industries valorize 10% of wheat bran, most of this antioxidant-rich byproduct gets fed to livestock. The objective of this study was to incorporate wheat bran into an extruded snack. Bran samples from hard red spring, soft white club cv. Bruehl, and purple wheat lines were added to cv. Waxy-Pen wheat flour (Triticum aestivum L.) at replacement concentrations of 0%, 12.5%, 25%, and 37.5% (w/w; n = 10). Extrudates were evaluated for antioxidant capacity, color, and physical properties. Results showed that high fiber concentrations altered several pasting properties, reduced expansion ratios (P<0.0001), and created denser products (P<0.0001), especially for white bran supplemented extrudates. Purple bran supplemented extrudates produced harder products compared to white and red bran treatments (P<0.0001). Extrudates produced with 37.5% (w/w) of each bran variety absorbed more water than the control with no added bran. The oxygen radical absorption capacity assay, expressed as Trolox Equivalents, showed that extrudates made with addition of red (37.5%) and purple (37.5%) bran had higher values compared to the other treatments; the control, red, and white bran treatments had less antioxidant activity after extrusion (P<0.0001) compared to purple bran supplemented extrudates. Purple and red brans may serve as viable functional ingredients in extruded foods given their higher antioxidant activities. Future studies could evaluate how bran variety and concentration, extruded shape, and flavor influence consumer acceptance. C1 [Fleischman, Emily F.; Kowalski, Ryan J.; Nguyen, Thuy; Li, Chongjun; Ganjyal, Girish; Ross, Carolyn F.] Washington State Univ, Sch Food Sci, Pullman, WA 99164 USA. [Morris, Craig F.] ARS, USDA, Western Wheat Qual Lab, Pullman, WA 99164 USA. RP Ross, CF (reprint author), Washington State Univ, Sch Food Sci, Pullman, WA 99164 USA. EM cfross@wsu.edu NR 54 TC 0 Z9 0 U1 11 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD NOV PY 2016 VL 81 IS 11 BP E2726 EP E2733 DI 10.1111/1750-3841.13511 PG 8 WC Food Science & Technology SC Food Science & Technology GA ED6GF UT WOS:000388953400009 ER PT J AU Shao, DY Wang, YL Huang, QS Shi, JL Yang, H Pan, ZL Jin, ML Zhao, HB Xu, XG AF Shao, Dongyan Wang, Yilin Huang, Qingsheng Shi, Junling Yang, Hui Pan, Zhongli Jin, Mingliang Zhao, Haobin Xu, Xiaoguang TI Cholesterol-Lowering Effects and Mechanisms in View of Bile Acid Pathway of Resveratrol and Resveratrol Glucuronides SO JOURNAL OF FOOD SCIENCE LA English DT Article DE bile acid; cholesterol lowering; excretion; resveratrol; resveratrol glucuronide ID HIGH-FAT DIETS; GENE-EXPRESSION; HAMSTERS; PLASMA; METABOLISM; INCREASES; LIVER; MICE; RECEPTORS; PRODUCT AB Resveratrol (Res) was previously reported to be capable of lowering plasma TC and LDL-C. The mechanism behind Res is not clearly understood, although it is presumed to have an effect on bile acid metabolism in the liver: a significant way in eliminating cholesterol from the body. As one of the major metabolites of Res in the liver, resveratrol glucuronides (Gres) is suspected to also contribute to the overall cholestrol-lowering activity of Res, which needs to be studied. In this research, when HepG2 steatosis hepatic cells were treated with Res and Gres at different concentration levels, Res and Gres showed similar activity in lowering cellular TC content. The presence of Res and Gres caused a significant increase in hepatic CYP7A1 and BSEP, indicating the increase in the synthesis and efflux of bile acids, respectively. The reduction of HMG-CoAR tied to a decrease in de novo synthesis of cholesterol and the increase of ABCG5 suggested the increase of direct efflux of cholesterol. All above variations reduced the hepatic cholesterol level, which triggered the significant enhancement of LDLR, illustrating the improvement of clearance of LDL-C from the plasma and prevention of atherosclerosis. Overall, this study demonstrated both Res and Gres might have capabilities in lowering hepatic cholesterol through increasing in the synthesis and efflux of bile acids, and decreasing in synthesis and increasing in the efflux of cholesterol. Gres would have preferred potential than Res because of its lower cytotoxicity, which indicated that the action of the metabolites should also be considered in the future studies. C1 [Shao, Dongyan; Wang, Yilin; Huang, Qingsheng; Shi, Junling; Yang, Hui; Jin, Mingliang; Zhao, Haobin; Xu, Xiaoguang] Northwestern Polytech Univ, Key Lab Space Biosci & Biotechnol, Sch Life Sci, Xian 710072, Shaanxi, Peoples R China. [Pan, Zhongli] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. [Pan, Zhongli] ARS, Hlth Proc Foods Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Shao, DY (reprint author), Northwestern Polytech Univ, Key Lab Space Biosci & Biotechnol, Sch Life Sci, Xian 710072, Shaanxi, Peoples R China. EM sjlshi2004@nwpu.edu.cn FU project of National Natural Science Funding [31471718]; National Key Technology RD Program [2015BAD16B02]; Agriculture Dept. of China [CARS-30]; Shaanxi Provincial Natural Science Foundation [2015JQ3083]; Fundamental Research Funds for the Central Universities [3102016QD075, 3102014JCQ15001]; China Postdoctoral Science Foundation [2014M562451] FX The authors would acknowledge financial support from the project of National Natural Science Funding (no. 31471718), National Key Technology R&D Program (no. 2015BAD16B02), the Agriculture Dept. of China (no. CARS-30), Shaanxi Provincial Natural Science Foundation (no. 2015JQ3083), Fundamental Research Funds for the Central Universities (no. 3102016QD075 and 3102014JCQ15001), and the China Postdoctoral Science Foundation (no. 2014M562451) There is no conflict of interest. NR 34 TC 0 Z9 0 U1 6 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD NOV PY 2016 VL 81 IS 11 BP H2841 EP H2848 DI 10.1111/1750-3841.13528 PG 8 WC Food Science & Technology SC Food Science & Technology GA ED6GF UT WOS:000388953400028 ER PT J AU Dean, LL Klevorn, CM Hess, BJ AF Dean, L. L. Klevorn, C. M. Hess, B. J. TI Minimizing the Negative Flavor Attributes and Evaluating Consumer Acceptance of Chocolate Fortified with Peanut Skin Extracts SO JOURNAL OF FOOD SCIENCE LA English DT Article DE bitter flavor; chocolate; natural antioxidants; peanut skins; phenolic compounds ID ANTIOXIDANT CAPACITY; PHENOLIC-COMPOUNDS; MILK CHOCOLATE; BITTER TASTE; COCOA POWDER; TOLERANCE; PRODUCTS; QUALITY; BEEF AB In recent years, there has been increased interest in antioxidant-rich products by consumers wanting to enhance the health benefits of their diet. Chocolate has been identified as a natural source of antioxidant compounds, which resulted in the development of polyphenol-enriched chocolate products that are now available commercially. This study investigated the use of phenolic compounds extracted from peanut skins as a novel antioxidant source for the enrichment of milk chocolate. The extracts were encapsulated with maltodextrin to lessen their bitterness. Antioxidant potential of the encapsulated peanut skin extracts was evaluated by the 2,2-diphenyl-1-picrylhydrazl radical quenching assay. Encapsulated peanut skins were found to have a corrected Trolox equivalency of 31.1 mu mol/g of chocolate up to 0.8% (w/w). To produce a product with an antioxidant content similar to that of dark chocolate yet which maintained the milder flavor of milk chocolate, the best estimate threshold of encapsulated peanut skin extract in chocolate was 0.9 % (w/w) based on the standard method (American Society of Testing Materials; ASTM E-679). Consumer liking of milk chocolate enhanced by adding subthreshold (0.8 % (w/w)) inclusion levels of encapsulated peanut skin extract was found to be at parity with milk chocolate as a control. C1 [Dean, L. L.] ARS, USDA, Market Qual & Handling Res Unit, Box 7624, Raleigh, NC 27695 USA. [Klevorn, C. M.] North Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. [Hess, B. J.] North Carolina State Univ, Dept Biol & Agr Engn, Raleigh, NC 27695 USA. RP Dean, LL (reprint author), ARS, USDA, Market Qual & Handling Res Unit, Box 7624, Raleigh, NC 27695 USA. EM Lisa.Dean@ars.usda.gov NR 41 TC 0 Z9 0 U1 10 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD NOV PY 2016 VL 81 IS 11 BP S2824 EP S2830 DI 10.1111/1750-3841.13533 PG 7 WC Food Science & Technology SC Food Science & Technology GA ED6GF UT WOS:000388953400006 ER PT J AU Vornhof-DeKrey, E Darland, D Ghribi, O Bundy, A Roemmich, J Claycombe, K AF Vornhof-DeKrey, Emilie Darland, Diane Ghribi, Othman Bundy, Amy Roemmich, James Claycombe, Kate TI Maternal low protein diet leads to placental angiogenic compensation via dysregulated M1/M2 macrophages and TNF alpha expression in Sprague-Dawley rats SO JOURNAL OF REPRODUCTIVE IMMUNOLOGY LA English DT Article DE iNKT cells; Angiogenesis; Fibroblast growth factor; M1 and M2 macrophages; Low protein diet ID ENDOTHELIAL GROWTH-FACTOR; NECROSIS-FACTOR-ALPHA; PRENATAL LOW-PROTEIN; FETAL-GROWTH; NKT CELLS; MEDIATES INFLAMMATION; ENDOCRINE REGULATION; TROPHOBLAST CELLS; TISSUE FACTOR; PREGNANCY AB A maternal low-protein (LP) diet in Sprague-Dawley rats results in low birth weight, rapid adipose tissue catch-up growth, adult obesity, and insulin resistance. The placenta functions to fulfill the fetus' nutrient demands. Adequate angiogenic factor concentrations help to ensure normal growth and vasculature development of the placenta and, in turn, optimum maternal-to-fetal nutrient delivery. Maternal malnutrition creates a proinflammatory environment that leads to inhibition of placental tissue growth. Therefore, we hypothesized that a maternal LP diet will lead to abnormal angiogenesis via dysregulation of immune cells resulting in increased secretion of proinflammatory cytokines and reduced angiogenic factor expression. Sprague-Dawley dams were fed 8% LP or 20% normal protein diets for 3 weeks prior to breeding and throughout pregnancy. Placenta from dams fed a LP diet weighed less; had increased M2 macrophages producing TNF alpha, decreased M1 macrophages and iNKT cells; greater angiogenic factor (FGF2, VEGFR-1, IGF2) expression and protein content, and greater CD31/PECAM (platelet endothelial cell adhesion molecule) expression. Prenatal protein restriction may induce the placenta to upregulate compensatory mechanisms of angiogenesis in order to meet the nutrient demands of the fetus. Published by Elsevier Ireland Ltd. C1 [Vornhof-DeKrey, Emilie; Bundy, Amy; Roemmich, James; Claycombe, Kate] USDA ARS, Human Nutr Res Ctr, Grand Forks, ND 58203 USA. [Vornhof-DeKrey, Emilie] Univ North Dakota, Sch Med & Hlth Sci, Dept Surg, Grand Forks, ND 58202 USA. [Darland, Diane] Univ North Dakota, Sch Med & Hlth Sci, Dept Biol, Grand Forks, ND 58202 USA. [Ghribi, Othman] Univ North Dakota, Sch Med & Hlth Sci, Dept Basic Sci, Grand Forks, ND 58202 USA. RP Claycombe, K (reprint author), USDA ARS, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave, N Grand Forks, ND 58203 USA. EM kate.claycombe@ars.usda.gov FU USDA Agricultural Research Services [3062-51000-052-00D] FX This work was funded by USDA Agricultural Research Service Project #3062-51000-052-00D. The contents of this publication do not necessarily reflect the views or policies of the United States Department of Agriculture or the Agricultural Research Service, nor does mention of trade names, commercial products, or organizations imply endorsement from the U.S. government. USDA is an equal opportunity provider and employer. NR 44 TC 0 Z9 0 U1 6 U2 6 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0165-0378 J9 J REPROD IMMUNOL JI J. Reprod. Immunol. PD NOV PY 2016 VL 118 BP 9 EP 17 DI 10.1016/j.jri.2016.08.009 PG 9 WC Immunology; Reproductive Biology SC Immunology; Reproductive Biology GA EE0QM UT WOS:000389284000002 ER PT J AU Sundermann, EE Katz, MJ Lipton, RB Lichtenstein, AH Derby, CA AF Sundermann, Erin E. Katz, Mindy J. Lipton, Richard B. Lichtenstein, Alice H. Derby, Carol A. TI A Brief Dietary Assessment Predicts Executive Dysfunction in an Elderly Cohort: Results from the Einstein Aging Study SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Article DE diet; cognition; executive function; race ID MILD COGNITIVE IMPAIRMENT; MEDITERRANEAN DIET; DEMENTIA; NUTRITION; ASSOCIATION; DECLINE; DISEASE; PEOPLE; REAP AB ObjectivesTo examine the association between diet and executive function, episodic memory and global verbal cognition in the Einstein Aging Study (EAS) cohort and determine whether race modifies this relationship. DesignCross-sectional. SettingCommunity. ParticipantsEAS participants without dementia who completed the Rapid Eating and Activity Assessment for Patients (REAP) (N = 492). MeasurementsThe previously validated REAP is based on the 2000 U.S. dietary guidelines. REAP scores were dichotomized as less-healthy (5000 ng L-1) than the concentrations in runoff from the shallow disk injection plots (<10 ng L-1). Additionally, the transport dynamics differed, with the majority of the estrogen loads from the surface broadcast plots occurring during the first rainfall event after application, whereas the majority of the loads from the shallow disk injection plots occurred more than 6 mo later during a hail storm event. Total estrogen loads were, on average, two orders of magnitude lower for shallow disk injection compared with surface broadcast. Independent of the method of manure application, 17 alpha-estradiol and estrone were preserved in the field for as long as 9 mo after application. Overall, injection of manure shows promise in reducing the potential for off-site losses of hormones from manure-amended soils. C1 [Mina, Odette; Gall, Heather E.] Penn State Univ, Dept Agr & Biol Engn, University Pk, PA 16802 USA. [Saporito, Louis S.; Kleinman, Peter J. A.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. RP Gall, HE (reprint author), Penn State Univ, Dept Agr & Biol Engn, University Pk, PA 16802 USA. EM heg12@psu.edu FU Penn State Institutes of Energy and the Environment; Northeast Sustainable Agricultural Research and Education (NESARE) program; Brazil Scientific Mobility Grant; College of Agricultural Sciences Undergraduate Research Program; Erickson Discovery Grant FX This research was supported, in part, by the Penn State Institutes of Energy and the Environment. Construction of the field plots and field management activities were funded, in part, by a grant from the Northeast Sustainable Agricultural Research and Education (NESARE) program. The authors thank Dr. Heather Karsten for providing with manure application information; Gordon Folmar, Nicolas Florentino Assalim dos Santos (supported by the Brazil Scientific Mobility Grant), Marc Gluberman (supported by the College of Agricultural Sciences Undergraduate Research Program), Alexandera Everhart (supported by the College of Agricultural Sciences Undergraduate Research Program), Daniel Schultz (supported by the College of Agricultural Sciences Undergraduate Research Program and Erickson Discovery Grant), and Dr. Malcom Taylor for their help in the field and laboratory; and three anonymous reviewers, whose comments and suggestions greatly improved the manuscript. NR 39 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD NOV-DEC PY 2016 VL 45 IS 6 BP 2007 EP 2015 DI 10.2134/jeq2016.05.0173 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA ED6DF UT WOS:000388944600024 PM 27898780 ER PT J AU Paiva, SR McManus, CM Slackburn, H AF Paiva, Samuel Rezende McManus, Concepta M. Slackburn, Harvey TI Conservation of animal genetic resources - A new tact SO LIVESTOCK SCIENCE LA English DT Article DE Conservation genetics; Gene banks; Livestock; Genetic resources; Genetic diversity ID CLIMATE-CHANGE; SELECTION; CATTLE; SHEEP; MANAGEMENT; IMPACT; BRAZIL AB For the past 20 years countries have initiated programs to sustainably conserve farm animal genetic resources. At the same time the growing need for increased animal productivity has emerged. Viewing gene banks and in vivo conservation in the context of food security, climate change, and product demand suggests the need for a more efficient use of these mechanisms to support sustainable productivity. Some advances have been made in developing and implementing in-vivo conservation programs, but those efforts appear to be predicated upon various types of government subsidies, which are subject, to policy changes. Given the in-vivo situation, it is suggested conservation efforts shift toward gene banks as the primary conservation mechanism. Globally, national gene banking efforts have increased and they have the capacity and potential to become more dynamic, incorporate different biological materials and facilitate increased use of genetic diversity. The next steps for gene banks are to better utilize information systems to integrate and store data from genetic/genomic assessments, cryopreservation, phenotypes and environmental conditions. These types of benefits plus the reduced conservation costs gene banks can speed the rate of conserving breeds while freeing the livestock sector to increase productivity with the breeds of their choosing. C1 [Paiva, Samuel Rezende] Embrapa Brazilian Agr Res Corp, Secretariat Int Affairs, Embrapa Labex US Program, Brasilia, DF, Brazil. [McManus, Concepta M.] Univ Brasilia, INCT Pecuaria, Fac Agron & Med Vet, Brasilia, DF, Brazil. [Slackburn, Harvey] ARS, USDA, Natl Anim Germplasm Program, Ft Collins, CO 80521 USA. RP Slackburn, H (reprint author), ARS, USDA, Natl Anim Germplasm Program, Ft Collins, CO 80521 USA. EM samuel.paiva@embrapa.br; concepta@unb.br; harvey.blackburn@ars.usda.gov RI McManus, Concepta/I-4356-2012 OI McManus, Concepta/0000-0002-1106-8962 FU USDA-ARS; Embrapa; UnB FX USDA-ARS, Embrapa, UnB. NR 46 TC 0 Z9 0 U1 7 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1871-1413 EI 1878-0490 J9 LIVEST SCI JI Livest. Sci. PD NOV PY 2016 VL 193 BP 32 EP 38 DI 10.1016/j.livsci.2016.09.010 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA ED3VP UT WOS:000388776900005 ER PT J AU Tako, E Bar, H Glahn, RP AF Tako, Elad Bar, Haim Glahn, Raymond P. TI The Combined Application of the Caco-2 Cell Bioassay Coupled with In Vivo (Gallus gallus) Feeding Trial Represents an Effective Approach to Predicting Fe Bioavailability in Humans SO NUTRIENTS LA English DT Review DE bioavailability; biofortification; iron; beans; pearl millet; screening tools; Gallus gallus; Caco-2 bioassay ID PHASEOLUS-VULGARIS L.; STAPLE FOOD CROPS; VITRO DIGESTION/CACO-2 MODEL; KENYAN COMPLEMENTARY FOODS; NONHEME-IRON-ABSORPTION; BEAN DIET PROVIDE; ASCORBIC-ACID; PHYTIC ACID; CULTURE MODEL; POLYPHENOLIC COMPOUNDS AB Research methods that predict Fe bioavailability for humans can be extremely useful in evaluating food fortification strategies, developing Fe-biofortified enhanced staple food crops and assessing the Fe bioavailability of meal plans that include such crops. In this review, research from four recent poultry (Gallus gallus) feeding trials coupled with in vitro analyses of Fe-biofortified crops will be compared to the parallel human efficacy studies which used the same varieties and harvests of the Fe-biofortified crops. Similar to the human studies, these trials were aimed to assess the potential effects of regular consumption of these enhanced staple crops on maintenance or improvement of iron status. The results demonstrate a strong agreement between the in vitro/in vivo screening approach and the parallel human studies. These observations therefore indicate that the in vitro/Caco-2 cell and Gallus gallus models can be integral tools to develop varieties of staple food crops and predict their effect on iron status in humans. The cost-effectiveness of this approach also means that it can be used to monitor the nutritional stability of the Fe-biofortified crop once a variety has released and integrated into the food system. These screening tools therefore represent a significant advancement to the field for crop development and can be applied to ensure the sustainability of the biofortification approach. C1 [Tako, Elad; Glahn, Raymond P.] ARS, USDA, Robert Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Bar, Haim] Univ Connecticut, Dept Stat, Storrs, CT 06269 USA. RP Tako, E (reprint author), ARS, USDA, Robert Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM et79@cornell.edu; haim.bar@uconn.edu; rpg3@cornell.edu NR 120 TC 0 Z9 0 U1 0 U2 0 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2072-6643 J9 NUTRIENTS JI Nutrients PD NOV PY 2016 VL 8 IS 11 AR 732 DI 10.3390/nu8110732 PG 25 WC Nutrition & Dietetics SC Nutrition & Dietetics GA ED2IE UT WOS:000388666400064 ER PT J AU Gorsich, EE Luis, AD Buhnerkempe, MG Grear, DA Portacci, K Miller, RS Webb, CT AF Gorsich, Erin E. Luis, Angela D. Buhnerkempe, Michael G. Grear, Daniel A. Portacci, Katie Miller, Ryan S. Webb, Colleen T. TI Mapping US cattle shipment networks: Spatial and temporal patterns of trade communities from 2009 to 2011 SO PREVENTIVE VETERINARY MEDICINE LA English DT Article DE Cattle shipment; Community detection; Dynamic network; Network analysis; Interstate Certificate of Veterinary; Inspection; US cattle industry ID INDIRECT CONTACT RATES; INFECTIOUS-DISEASES; MOUTH-DISEASE; MOVEMENTS; SPREAD; BEEF; CALIFORNIA; FRANCE; HERDS AB The application of network analysis to cattle shipments broadens our understanding of shipment patterns beyond pairwise interactions to the network as a whole. Such a quantitative description of cattle shipments in the U.S. can identify trade communities, describe temporal shipment patterns, and inform the design of disease surveillance and control strategies. Here, we analyze a longitudinal dataset of beef and dairy cattle shipments from 2009 to 2011 in the United States to characterize communities within the broader cattle shipment network, which are groups of counties that ship mostly to each other. Because shipments occur over time, we aggregate the data at various temporal scales to examine the consistency of network and community structure over time. Our results identified nine large (>50 counties) communities based on shipments of beef cattle in 2009 aggregated into an annual network and nine large communities based on shipments of dairy cattle. The size and connectance of the shipment network was highly dynamic; monthly networks were smaller than yearly networks and revealed seasonal shipment patterns consistent across years. Comparison of the shipment network over time showed largely consistent shipping patterns, such that communities identified on annual networks of beef and diary shipments from 2009 still represented 41-95% of shipments in monthly networks from 2009 and 41-66% of shipments from networks in 2010 and 2011. The temporal aspects of cattle shipments suggest that future applications of the U.S. cattle shipment network should consider seasonal shipment patterns. However, the consistent within-community shipping patterns indicate that yearly communities could provide a reasonable way to group regions for management. (C) 2016 Elsevier B.V. All rights reserved. C1 [Gorsich, Erin E.; Webb, Colleen T.] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Luis, Angela D.] Univ Montana, Dept Ecosyst & Conservat Sci, Missoula, MT 59812 USA. [Buhnerkempe, Michael G.] Univ Calif Los Angeles, Dept Ecol & Evolutionary Biol, Los Angeles, CA USA. [Buhnerkempe, Michael G.] NIH, Fogarty Int Ctr, Bldg 10, Bethesda, MD 20892 USA. [Grear, Daniel A.; Portacci, Katie; Miller, Ryan S.] USDA APHIS Vet Serv, Ctr Epidemiol & Anim Hlth, Ft Collins, CO USA. RP Gorsich, EE (reprint author), Oregon State Univ, Dept Biomed Sci, Corvallis, OR 97331 USA. EM eringorsich@gmail.com OI Miller, Ryan/0000-0003-3892-0251 FU Foreign Animal Disease Modeling Program, Science and Technology Directorate; U.S. Department of Homeland Security [ST-108-000017]; USDA [13-9208-0345-CA]; Research and Policy for Infectious Disease Dynamics (RAPIDD) program of the Science and Technology Directorate, Department of Homeland Security; Fogarty International Center, National Institutes of Health [HSHQDC-09-X-00135] FX Funding was provided by the Foreign Animal Disease Modeling Program, Science and Technology Directorate, U.S. Department of Homeland Security (Grant ST-108-000017) and a USDA Cooperative Agreement 13-9208-0345-CA. MGB and CTW were supported by the Research and Policy for Infectious Disease Dynamics (RAPIDD) program of the Science and Technology Directorate, Department of Homeland Security, and Fogarty International Center, National Institutes of Health; through interagency agreement #HSHQDC-09-X-00135. Data were provided by the U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services. However, the analyses, views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the regulatory opinions, official policies, either expressed or implied, of the USDA-APHIS-Veterinary Services or the U.S. Department of Homeland Security. We also thank the state veterinarians and staff whose cooperation and effort made the data collection possible. NR 36 TC 0 Z9 0 U1 5 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-5877 EI 1873-1716 J9 PREV VET MED JI Prev. Vet. Med. PD NOV 1 PY 2016 VL 134 BP 82 EP 91 DI 10.1016/j.prevetmed.2016.09.023 PG 10 WC Veterinary Sciences SC Veterinary Sciences GA ED8CV UT WOS:000389100500009 PM 27836049 ER PT J AU Merrill, KR Coleman, CE Meyer, SE Leger, EA Collins, KA AF Merrill, Keith R. Coleman, Craig E. Meyer, Susan E. Leger, Elizabeth A. Collins, Katherine A. TI DEVELOPMENT OF SINGLE-NUCLEOTIDE POLYMORPHISM MARKERS FOR BROMUS TECTORUM (POACEAE) FROM A PARTIALLY SEQUENCED TRANSCRIPTOME SO APPLICATIONS IN PLANT SCIENCES LA English DT Article DE Bromus tectorum; cheatgrass; invasive; Poaceae; single-nucleotide polymorphism (SNP) development ID POPULATION GENETIC-ANALYSIS; INBREEDING ANNUAL GRASS; CHEATGRASS; RANGE AB Premise of the study: Bromus tectorum (Poaceae) is an annual grass species that is invasive in many areas of the world but most especially in the U.S. Intermountain West. Single-nucleotide polymorphism (SNP) markers were developed for use in investigating the geospatial and ecological diversity of B. tectorum in the Intermountain West to better understand the mechanisms behind its successful invasion. Methods and Results: Normalized cDNA libraries from six diverse B. tectorum individuals were pooled and sequenced using 454 sequencing. Ninety-five SNP assays were developed for use on 96.96 arrays with the Fluidigm EP1 genotyping platform. Verification of the 95 SNPs by genotyping 251 individuals from 12 populations is reported, along with amplification data from four related Bromus species. Conclusions: These SNP markers are polymorphic across populations of B. tectorum, are optimized for high-throughput applications, and may be applicable to other, related Bromus species. C1 [Merrill, Keith R.; Meyer, Susan E.] US Forest Serv, Shrub Sci Lab, Rocky Mt Res Stn, 735 North 500 East, Provo, UT 84606 USA. [Merrill, Keith R.; Coleman, Craig E.; Collins, Katherine A.] Brigham Young Univ, Dept Plant & Wildlife Sci, 4105 Life Sci Bldg, Provo, UT 84602 USA. [Leger, Elizabeth A.] Univ Nevada, Dept Nat Resources & Environm Sci, 1664 N Virginia St,MS 186, Reno, NV 89557 USA. RP Coleman, CE (reprint author), Brigham Young Univ, Dept Plant & Wildlife Sci, 4105 Life Sci Bldg, Provo, UT 84602 USA. EM craig_coleman@byu.edu FU Joint Fire Sciences Program [2007-1-3-10]; Cooperative State Research, Education, and Extension Service (CSREES) National Resource Initiative Biology of Weedy and Invasive Species Program [2008-35320-18677]; University of Nevada, Reno FX The authors thank S. Harrison for his help with tissue collection and preparation. This research was funded in part through grants from the Joint Fire Sciences Program (2007-1-3-10) and the Cooperative State Research, Education, and Extension Service (CSREES) National Resource Initiative Biology of Weedy and Invasive Species Program (2008-35320-18677), and through support from the University of Nevada, Reno. NR 15 TC 0 Z9 0 U1 6 U2 6 PU BOTANICAL SOC AMER INC PI ST LOUIS PA PO BOX 299, ST LOUIS, MO 63166-0299 USA SN 2168-0450 J9 APPL PLANT SCI JI Appl. Plant Sci. PD NOV PY 2016 VL 4 IS 11 AR 1600068 DI 10.3732/apps.1600068 PG 6 WC Plant Sciences SC Plant Sciences GA ED0CD UT WOS:000388509700001 ER PT J AU Kirs, M Caffaro, RA Wong, M Harwood, VJ Moravcik, P Fujioka, RS AF Kirs, Marek Caffaro-Filho, Roberto A. Wong, Mayee Harwood, Valerie J. Moravcik, Philip Fujioka, Roger S. TI Human-Associated Bacteroides spp. and Human Polyomaviruses as Microbial Source Tracking Markers in Hawaii SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID HUMAN FECAL POLLUTION; POLYMERASE-CHAIN-REACTION; REAL-TIME PCR; FRESH-WATER; SUNLIGHT INACTIVATION; INDICATOR BACTERIA; ESCHERICHIA-COLI; GENETIC-MARKERS; QUANTITATIVE DETECTION; ENVIRONMENTAL WATERS AB Identification of sources of fecal contaminants is needed to (i) determine the health risk associated with recreational water use and (ii) implement appropriate management practices to mitigate this risk and protect the environment. This study evaluated human-associated Bacteroides spp. (HF183TaqMan) and human polyomavirus (HPyV) markers for host sensitivity and specificity using human and animal fecal samples collected in Hawaii. The decay rates of those markers and indicator bacteria were identified in marine and freshwater microcosms exposed and not exposed to sunlight, followed by field testing of the usability of the molecular markers. Both markers were strongly associated with sewage, although the cross-reactivity of the HF183TaqMan (also present in 82% of canine [n = 11], 30% of mongoose [n = 10], and 10% of feline [n = 10] samples) needs to be considered. Concentrations of HF183TaqMan in human fecal samples exceeded those in cross-reactive animals at least 1,000-fold. In the absence of sunlight, the decay rates of both markers were comparable to the die-off rates of enterococci in experimental freshwater and marine water microcosms. However, in sunlight, the decay rates of both markers were significantly lower than the decay rate of enterococci. While both markers have their individual limitations in terms of sensitivity and specificity, these limitations can be mitigated by using both markers simultaneously; ergo, this study supports the concurrent use of HF183TaqMan and HPyV markers for the detection of sewage contamination in coastal and inland waters in Hawaii. C1 [Kirs, Marek; Caffaro-Filho, Roberto A.; Wong, Mayee; Moravcik, Philip; Fujioka, Roger S.] Univ Hawaii, Water Resources Res Ctr, Honolulu, HI 96822 USA. [Harwood, Valerie J.] Univ S Florida, Div Integrat Biol, Tampa, FL USA. [Caffaro-Filho, Roberto A.] Univ Fed Alagoas, Ctr Technol, Maceio, Alagoas, Brazil. [Wong, Mayee] USDA, Anim & Plant Hlth Inspect Serv, Los Angeles, CA USA. RP Kirs, M (reprint author), Univ Hawaii, Water Resources Res Ctr, Honolulu, HI 96822 USA. EM kirs@hawaii.edu FU Water Resources Research Institute Program (WRRIP) [2012HI355B]; Water Resources Research Center at University of Hawaii FX Funding was provided by a grant (2012HI355B) through the Water Resources Research Institute Program (WRRIP) and supported by the Water Resources Research Center at the University of Hawaii. NR 62 TC 0 Z9 0 U1 5 U2 5 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD NOV PY 2016 VL 82 IS 22 BP 6757 EP 6767 DI 10.1128/AEM.01959-16 PG 11 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA EA9KP UT WOS:000386963000026 PM 27613686 ER PT J AU Li, W Evans, JD Huang, Q Rodriguez-Garcia, C Liu, J Hamilton, M Grozinger, CM Webster, TC Su, S Chen, YP AF Li, Wenfeng Evans, Jay D. Huang, Qiang Rodriguez-Garcia, Cristina Liu, Jie Hamilton, Michele Grozinger, Christina M. Webster, Thomas C. Su, Songkun Chen, Yan Ping TI Silencing the Honey Bee (Apis mellifera) Naked Cuticle Gene (nkd) Improves Host Immune Function and Reduces Nosema ceranae Infections SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID INTESTINAL EPITHELIAL-CELLS; WINTER COLONY LOSSES; DOUBLE-STRANDED-RNA; MICROSPORIDIAN PARASITE; UNITED-STATES; INFORMED PARTNERSHIP; VIRUS-INFECTION; NATIONAL-SURVEY; COLLAPSE; WNT AB Nosema ceranae is a new and emerging microsporidian parasite of European honey bees, Apis mellifera, that has been implicated in colony losses worldwide. RNA interference (RNAi), a posttranscriptional gene silencing mechanism, has emerged as a potent and specific strategy for controlling infections of parasites and pathogens in honey bees. While previous studies have focused on the silencing of parasite/pathogen virulence factors, we explore here the possibility of silencing a host factor as a mechanism for reducing parasite load. Specifically, we used an RNAi strategy to reduce the expression of a honey bee gene, naked cuticle (nkd), which is a negative regulator of host immune function. Our studies found that nkd mRNA levels in adult bees were upregulated by N. ceranae infection (and thus, the parasite may use this mechanism to suppress host immune function) and that ingestion of double-stranded RNA (dsRNA) specific to nkd efficiently silenced its expression. Furthermore, we found that RNA-imediated knockdown of nkd transcripts in Nosema-infected bees resulted in upregulation of the expression of several immune genes (Abaecin, Apidaecin, Defensin-1, and PGRP-S2), reduction of Nosema spore loads, and extension of honey bee life span. The results of our studies clearly indicate that silencing the host nkd gene can activate honey bee immune responses, suppress the reproduction of N. ceranae, and improve the overall health of honey bees. This study represents a novel host-derived therapeutic for honey bee disease treatment that merits further exploration. C1 [Li, Wenfeng; Evans, Jay D.; Huang, Qiang; Liu, Jie; Hamilton, Michele; Chen, Yan Ping] USDA ARS, Bee Res Lab, Beltsville, MD 20705 USA. [Huang, Qiang] Univ Bern, Inst Bee Hlth, Vetsuisse Fac, Bern, Switzerland. [Rodriguez-Garcia, Cristina] Ctr Apicola Marchamalo IRIAF, Guadalajara, Spain. [Grozinger, Christina M.] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. [Webster, Thomas C.] Kentucky State Univ, Coll Agr Food Sci & Sustainable Syst, Frankfort, KY USA. [Su, Songkun] Fujian Agr & Forestry Univ, Coll Bee Sci, Fuzhou, Peoples R China. RP Chen, YP (reprint author), USDA ARS, Bee Res Lab, Beltsville, MD 20705 USA. EM judy.chen@ars.usda.gov FU USDA-NIFA [2014-67013-21784]; INIA-FEDER [RTA2012-00072-C02-01] FX This work, including the efforts of Yan Ping Chen, Jay D. Evans, Christina M. Grozinger, and Thomas C. Webster, was funded by USDA-NIFA (2014-67013-21784). This work, including the efforts of Cristina Rodriguez-Garcia, was funded by INIA-FEDER (RTA2012-00072-C02-01). NR 73 TC 0 Z9 0 U1 13 U2 13 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD NOV PY 2016 VL 82 IS 22 BP 6779 EP 6787 DI 10.1128/AEM.02105-16 PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA EA9KP UT WOS:000386963000028 PM 27613683 ER PT J AU Trachsel, J Bayles, DO Looft, T Levine, UY Allen, HK AF Trachsel, Julian Bayles, Darrell O. Looft, Torey Levine, Uri Y. Allen, Heather K. TI Function and Phylogeny of Bacterial Butyryl Coenzyme A:Acetate Transferases and Their Diversity in the Proximal Colon of Swine SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID T-CELL GENERATION; CHAIN FATTY-ACID; GUT MICROBIOTA; SODIUM-BUTYRATE; INFLAMMATION; COMMENSAL; PCR; METABOLITES; SALMONELLA; INHIBITION AB Studying the host-associated butyrate-producing bacterial community is important, because butyrate is essential for colonic homeostasis and gut health. Previous research has identified the butyryl coenzyme A (CoA):acetate-CoA transferase (EC 2.3.8.3) as a gene of primary importance for butyrate production in intestinal ecosystems; however, this gene family (but) remains poorly defined. We developed tools for the analysis of butyrate-producing bacteria based on 12 putative but genes identified in the genomes of nine butyrate-producing bacteria obtained from the swine intestinal tract. Functional analyses revealed that eight of these genes had strong But enzyme activity. When but paralogues were found within a genome, only one gene per genome encoded strong activity, with the exception of one strain in which no gene encoded strong But activity. Degenerate primers were designed to amplify the functional but genes and were tested by amplifying environmental but sequences from DNA and RNA extracted from swine colonic contents. The results show diverse but sequences from swine-associated butyrate-producing bacteria, most of which clustered near functionally confirmed sequences. Here, we describe tools and a framework that allow the bacterial butyrate-producing community to be profiled in the context of animal health and disease. C1 [Trachsel, Julian; Looft, Torey; Levine, Uri Y.; Allen, Heather K.] ARS, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Trachsel, Julian] Iowa State Univ, Interdept Microbiol Grad Program, Ames, IA USA. [Bayles, Darrell O.] ARS, Infect Bacterial Dis Res Unit, Natl Anim Dis Ctr, Ames, IA USA. [Levine, Uri Y.] Novozymes Biol Inc, Salem, VA USA. RP Allen, HK (reprint author), ARS, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. EM heather.allen@ars.usda.gov FU USDA | Agricultural Research Service (ARS) FX This work was funded by the USDA | Agricultural Research Service (ARS). NR 51 TC 0 Z9 0 U1 3 U2 3 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD NOV PY 2016 VL 82 IS 22 BP 6788 EP 6798 DI 10.1128/AEM.02307-16 PG 11 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA EA9KP UT WOS:000386963000029 PM 27613689 ER PT J AU Gobster, PH Floress, K Westphal, LM Watkins, CA Vining, J Wali, A AF Gobster, Paul H. Floress, Kristin Westphal, Lynne M. Watkins, Cristy A. Vining, Joanne Wali, Alaka TI Resident and user support for urban natural areas restoration practices SO BIOLOGICAL CONSERVATION LA English DT Article DE Restoration success; Social acceptability; Land management practices; Beliefs; Gender ID SUBURBAN DEER MANAGEMENT; ECOLOGICAL RESTORATION; PUBLIC SUPPORT; WEED MANAGEMENT; GREEN SPACE; ATTITUDES; GENDER; ACCEPTABILITY; PERCEPTIONS; LANDHOLDERS AB Public support is important to the success of natural areas restoration programs. Support can be especially critical in urban settings where stakeholders recreate in or reside near natural areas but may lack familiarity with practices for managing ecological processes. Surveys of on-site recreationists and nearby residents (N = 888) of 11 Chicago metropolitan natural areas were used to assess support for eight different practices commonly used in oak woodland restoration. Support generally ranged in relation to the level or intensity of management intervention, from more than 90% of the sample supporting the planting of native seeds and plants to just 32% supporting the use of herbicides to control undesired vegetation. On-site users and nearby residents who believed that a restoration practice was being used at the site they visited and/or lived near were much more likely to support the use of that practice than those who did not believe or did not know whether it was being used. These belief variables were the most important predictors in binary logistic regression models of restoration support, though gender (female) also significantly decreased the likelihood of supporting most high-intervention practices. Beyond these findings, results also suggest that support should be viewed as a multidimensional concept that involves perceptual, demographic, and structural components which often differ for different practices. Managers can use the information provided here to increase their understanding of the relative nature of restoration support and devise holistic social-ecological strategies to achieve restoration success. Published by Elsevier Ltd. C1 [Gobster, Paul H.; Floress, Kristin; Westphal, Lynne M.] US Forest Serv, Northern Res Stn, 1033 Univ Pl 360, Evanston, IL 60201 USA. [Watkins, Cristy A.] Univ Michigan, Sch Nat Resources & Environm, Ann Arbor, MI 48109 USA. [Vining, Joanne] Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL USA. [Wali, Alaka] Field Museum Nat Hist, Integrat Res Ctr, 1400 S Lake Shore Dr, Chicago, IL 60605 USA. RP Gobster, PH (reprint author), US Forest Serv, Northern Res Stn, 1033 Univ Pl 360, Evanston, IL 60201 USA. EM pgobster@fs.fed.us; kfloress@fs.fed.us; lwestphal@fs.fed.us; watkinsc@umich.edu; jvining@illinois.edu; awali@fieldmuseum.org FU U.S. National Science Foundation [DEB-BE-0909451] FX Support for this research came in part through a grant from the U.S. National Science Foundation (DEB-BE-0909451), "Coupled Natural Human Systems in the Chicago Wilderness: Evaluating the Biodiversity and Social Outcomes of Different Models of Restoration Planning." We thank the three anonymous peer reviewers for their helpful comments in revising our original submission. NR 83 TC 0 Z9 0 U1 9 U2 9 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0006-3207 EI 1873-2917 J9 BIOL CONSERV JI Biol. Conserv. PD NOV PY 2016 VL 203 BP 216 EP 225 DI 10.1016/j.biocon.2016.09.025 PG 10 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA EC5EO UT WOS:000388156100026 ER PT J AU Scudiero, E Skaggs, TH Corwin, DL AF Scudiero, Elia Skaggs, Todd H. Corwin, Dennis L. TI Comparative regional-scale soil salinity assessment with near-ground apparent electrical conductivity and remote sensing canopy reflectance SO ECOLOGICAL INDICATORS LA English DT Article DE Soil salinity mapping; Apparent soil electrical conductivity; Spatial variability; Remote sensing; ANOCOVA ID SPATIAL VARIATION; MODEL; SUSTAINABILITY; PRECIPITATION; AGRICULTURE; VARIABILITY; VALIDATION; PREDICTION; PROTOCOLS; VALLEY AB Soil salinity is recognized worldwide as a major threat to agriculture, particularly in arid and semi-arid regions. Producers and decision makers need updated and accurate maps of salinity in agronomically and environmentally relevant ranges (i.e., <20 dS m(-1), when salinity is measured as electrical conductivity of the saturation extract, ECe). State-of-the-art approaches for creating accurate ECe maps beyond field scale (i.e., 1 km(2)) include: (i) Analysis Of Covariance (ANOCOVA) of near-ground measurements of apparent soil electrical conductivity (ECa) and (ii) regression modeling of multi-year remote sensing canopy reflectance and other co-variates (e.g., crop type, annual rainfall). This study presents a comparison of the two approaches to establish their viability and utility. The approaches were tested using 22 fields (total 542 ha) located in California's western San Joaquin Valley. In 2013 ECa-directed soil sampling resulted in the collection of 267 soil samples across the 22 fields, which were analyzed for ECe, ranging from 0 to 38.6 dS m(-1). The ANOCOVA ECa-ECe model returned a coefficient of determination (R-2) of 0.87 and root mean square prediction error (RMSPE) of 3.05 dS m(-1). For the remote sensing approach seven years (2007-2013) of Landsat 7 reflectance were considered. The remote sensing salinity model had R-2 0.73 and RMSPE = 3.63 dS m(-1). The robustness of the models was tested with a leave-one-field-out (lofo) cross-validation to assure maximum independence between training and validation datasets. For the ANOCOVA model, lofo cross-validation provided a range of scenarios in terms of RMSPE. The worst, median, and best fit scenarios provided global cross-validation R-2 of 0.52, 0.80, and 0.81, respectively. The lofo cross-validation for the remote sensing approach returned a R-2 of 0.65. The ANOCOVA approach performs particularly well at ECe values <10 dS m(-1), but requires extensive field work. Field work is reduced considerably with the remote sensing approach, but due to the larger errors at low ECe values, the methodology is less suitable for crop selection, and other practices that require accurate knowledge of salinity variation within a field, making it more useful for assessing trends in salinity across a regional scale. The two models proved to be viable solutions at large spatial scales, with the ANOCOVA approach more appropriate for multiple-field to landscape scales (1-10 km(2)) and the remote sensing approach best for landscape to regional scales (>10 km(2)). Published by Elsevier Ltd. C1 [Scudiero, Elia; Skaggs, Todd H.; Corwin, Dennis L.] USDA ARS, US Salin Lab, 450 West Big Springs Rd, Riverside, CA 92507 USA. RP Scudiero, E (reprint author), USDA ARS, US Salin Lab, 450 West Big Springs Rd, Riverside, CA 92507 USA. EM elia.scudiero@ars.usda.gov OI Scudiero, Elia/0000-0003-4944-721X FU Office of Naval Research (FMMI) [3200001344] FX Partial funding for this research was from the Office of Naval Research (FMMI number: 3200001344). The authors wish to acknowledge the numerous hours of diligent technical work that were performed in the field and in the laboratory by several technicians whose efforts and conscientiousness were crucial to obtaining the data presented, including Wes Clary, Kevin Yemoto, and Michael Bagtang. The authors specifically acknowledge the dedication and technical professionalism of Wes Clary who was crucial in locating field sites in the San Joaquin Valley, conducting ECa surveys, and analyzing soil samples and Kevin Yemoto who provided technical expertise with GIS and laboratory analyses. NR 45 TC 2 Z9 2 U1 10 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1470-160X EI 1872-7034 J9 ECOL INDIC JI Ecol. Indic. PD NOV PY 2016 VL 70 SI SI BP 276 EP 284 DI 10.1016/j.ecolind.2016.06.015 PG 9 WC Biodiversity Conservation; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA ED3YU UT WOS:000388785200025 ER PT J AU Cale, JA Klutsch, JG Erbilgin, N Negron, JF Castello, JD AF Cale, Jonathan A. Klutsch, Jennifer G. Erbilgin, Nadir Negron, Jose F. Castello, John D. TI Using structural sustainability for forest health monitoring and triage: Case study of a mountain pine beetle (Dendroctonus ponderosae)-impacted landscape SO ECOLOGICAL INDICATORS LA English DT Article DE Baseline mortality analysis; Forest structure; Forest disturbance; Climate change; Bark beetle ID LODGEPOLE PINE; CLIMATE-CHANGE; FIRE BEHAVIOR; BOREAL FOREST; BRITISH-COLUMBIA; RANGE EXPANSION; NORTH-AMERICA; DYNAMICS; EPIDEMIC; STANDS AB Heavy disturbance-induced mortality can negatively impact forest biota, functions, and services by drastically altering the forest structures that create stable environmental conditions. Disturbance impacts on forest structure can be assessed using structural sustainability the degree of balance between living and dead portions of a tree population's size-class distribution which is the basis of baseline mortality analysis. This analysis uses a conditionally calibrated reference level (i.e., baseline mortality) against which to detect significant mortality deviations without need for historical references. Recently, a structural sustainability index was developed by parameterizing results of this analysis to allow spatial and temporal comparisons within or among forested landscapes. The utility of this index as a tool for forest health monitoring programs and triage decisions has not been examined. Here, we investigated this utility by retrospectively analyzing the structural sustainability of a mountain pine beetle (Dendroctonus ponderosae)-impacted, lodgepole pine (Pinus contorta)-dominated landscape annually from 2000 to 2006 as well as among watersheds. We show that temporal patterns of structural sustainability at the landscape-level reflect accumulating beetle-induced mortality as well as beetle-lodgepole pine ecology. At the watershed-level, this sustainability spatially varied with 2006 beetle-induced mortality. Further, structural sustainability satisfies key criteria for effective indicators of ecosystem change. We conclude that structural sustainability is a viable tool upon which to base or supplement forest health monitoring and triage programs, and could potentially increase the efficacy of such programs under current and future climate change-associated disturbance patterns. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Cale, Jonathan A.; Klutsch, Jennifer G.; Erbilgin, Nadir] Univ Alberta, Dept Renewable Resources, 4-42 Earth Sci Bldg, Edmonton, AB T6G 2E3, Canada. [Negron, Jose F.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. [Castello, John D.] SUNY Coll Environm Sci & Forestry, Dept Environm & Forest Biol, Syracuse, NY 13210 USA. RP Cale, JA (reprint author), Univ Alberta, Dept Renewable Resources, 4-42 Earth Sci Bldg, Edmonton, AB T6G 2E3, Canada. EM jacale@ualberta.ca OI Cale, Jonathan/0000-0002-8902-2032 FU Natural Sciences and Engineering Research Council of Canada; Izaak Walton Killam Memorial Scholarship FX This work was partially supported by Natural Sciences and Engineering Research Council of Canada (Discovery grant to NE) and the Izaak Walton Killam Memorial Scholarship (to JGK). We thank Cortney D'Angelo as well as two anonymous reviewers for their comments on the manuscript. NR 52 TC 1 Z9 1 U1 8 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1470-160X EI 1872-7034 J9 ECOL INDIC JI Ecol. Indic. PD NOV PY 2016 VL 70 SI SI BP 451 EP 459 DI 10.1016/j.ecolind.2016.06.020 PG 9 WC Biodiversity Conservation; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA ED3YU UT WOS:000388785200041 ER PT J AU Martinez, SA Tuttle, KM Takebayashi, Y Seo, M Campbell, KG Steber, CM AF Martinez, Shantel A. Tuttle, Keiko M. Takebayashi, Yumiko Seo, Mitsunori Campbell, Kimberly Garland Steber, Camille M. TI The wheat ABA hypersensitive ERA8 mutant is associated with increased preharvest sprouting tolerance and altered hormone accumulation SO EUPHYTICA LA English DT Article DE Abscisic acid; Gibberellin; Auxin; Wheat; Preharvest sprouting; Seed dormancy ID TRITICUM-AESTIVUM L.; ARABIDOPSIS-THALIANA SEEDS; ACID SIGNAL-TRANSDUCTION; WHITE WINTER-WHEAT; ABSCISIC-ACID; GRAIN DORMANCY; PROTEIN-KINASES; HIGH-TEMPERATURE; GENE-EXPRESSION; COMMON WHEAT AB Wheat preharvest sprouting (PHS) is the germination of mature grain on the mother plant when rain occurs before harvest. Higher abscisic acid (ABA) hormone levels and sensitivity are associated with higher seed dormancy and PHS tolerance. Consistent with this, the ABA hypersensitive ENHANCED RESPONSE TO ABA8 (ERA8) mutant resulted in increased dormancy and PHS tolerance in soft white spring wheat 'Zak'. ERA8 seeds were initially less responsive to germination-rescue by the hormone gibberellin (GA). ERA8 gained GA and lost ABA sensitivity more slowly than wild-type during dormancy loss through after-ripening and cold imbibition. This study examined if increased ABA sensitivity in ERA8 likely resulted from increased ABA signaling or increased ABA hormone levels. Zak ERA8 had higher initial grain dormancy although endogenous embryo ABA levels were similar in Zak ERA8 and wild-type, suggesting that increased dormancy was due to increased ABA signaling rather than increased ABA accumulation. ABA levels declined with Zak ERA8 after-ripening, suggesting that ABA turnover is not defective. Elevated ERA8 dormancy was also associated with increased embryonic jasmonic acid-Ile and aleurone indole-3-acetic acid (IAA) levels. The possible implication that other plant hormones may influence wheat seed dormancy and germination are discussed. C1 [Martinez, Shantel A.; Tuttle, Keiko M.; Campbell, Kimberly Garland; Steber, Camille M.] Washington State Univ, Mol Plant Sci, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Takebayashi, Yumiko; Seo, Mitsunori] RIKEN, Ctr Sustainable Resource Sci, Yokohama, Kanagawa 2300045, Japan. [Campbell, Kimberly Garland; Steber, Camille M.] Washington State Univ, USDA, ARS, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. RP Steber, CM (reprint author), Washington State Univ, Mol Plant Sci, Dept Crop & Soil Sci, Pullman, WA 99164 USA. EM csteber@wsu.edu RI Takebayashi, Yumiko/C-8190-2017; OI Takebayashi, Yumiko/0000-0003-4207-297X; Steber, Camille/0000-0001-6255-7670 FU National Science Foundation's East Asia and Pacific Summer Institute Award [1209878]; Japanese Society for the Promotion of Science Summer Internship Award; Washington Grain Alliance; USDA-ARS FX This work was funded by the National Science Foundation's East Asia and Pacific Summer Institute Award grant 1209878 (to K.M.T.), the Japanese Society for the Promotion of Science Summer Internship Award (to K.M.T. and M.S.), the Washington Grain Alliance (to C.M.S.), and the USDA-ARS (to C.M.S. and K.G.C.). NR 85 TC 0 Z9 0 U1 9 U2 9 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD NOV PY 2016 VL 212 IS 2 BP 229 EP 245 DI 10.1007/s10681-016-1763-6 PG 17 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA EC5JI UT WOS:000388170500006 ER PT J AU Uchimiya, M Ni, XZ Wang, ML AF Uchimiya, Minori Ni, Xinzhi Wang, Ming Li TI Structure-reactivity relationships between the fluorescent chromophores and antioxidant activity of grain and sweet sorghum seeds SO FOOD SCIENCE & NUTRITION LA English DT Article DE Cereal; colorimetric method; proanthocyanidin; sweet sorghum; transition metal ID TANNIN; IRON AB Polyphenolic structures are the putative cause of a variety of seed functions including bird/insect resistance and antioxidant activity. Structure-reactivity relationships are necessary to understand the influence of polyphenolic chromophore structures on the tannin content and free radical quenching ability determined by the traditional calorimetric methods. This study investigated the relationships between the structural attributes of fluorescent chromophore and the following seed characterization methods: procyanidin (by acid-butanol assay) and flavonoid (by vanillin assay) contents, radical quenching (by DPPH assay), electron-donating capacity (by Fe-III reduction), and lambda(max) (by UV/visible spectrophotometry). Distinctively different response was observed for different seed categories: U.S. grain sorghum hybrids, African grain sorghum, and sweet sorghum. The U.S. grain sorghum varieties (low-tannin to maximize the livestock digestion) responded only to the DPPH assay. For sweet sorghum and African grain sorghum, linear correlation was observed between (1) the antioxidant activity (2) the amounts of procyanidins and flavonoids, and (2) the aromaticity of fingerprint fluorescent structures. C1 [Uchimiya, Minori] ARS, USDA, Southern Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [Ni, Xinzhi] ARS, USDA, Crop Genet & Breeding Res Unit, 2747 Davis Rd, Tifton, GA 31793 USA. [Wang, Ming Li] ARS, USDA, Plant Genet Resources Conservat Unit, Griffin, GA 30223 USA. RP Uchimiya, M (reprint author), ARS, USDA, Southern Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM sophie.uchimiya@ars.usda.gov NR 19 TC 0 Z9 0 U1 3 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2048-7177 J9 FOOD SCI NUTR JI Food Sci. Nutr. PD NOV PY 2016 VL 4 IS 6 BP 811 EP 817 DI 10.1002/fsn3.350 PG 7 WC Food Science & Technology SC Food Science & Technology GA EC5YB UT WOS:000388212100004 PM 27826430 ER PT J AU McHugh, T AF McHugh, Tara TI How Nut and Seed Butters Are Processed SO FOOD TECHNOLOGY LA English DT Editorial Material C1 [McHugh, Tara] ARS, USDA, Albany, CA 94710 USA. RP McHugh, T (reprint author), ARS, USDA, Albany, CA 94710 USA. EM tara.mchugh@ars.usda.gov NR 0 TC 0 Z9 0 U1 3 U2 3 PU INST FOOD TECHNOLOGISTS PI CHICAGO PA 525 WEST VAN BUREN, STE 1000, CHICAGO, IL 60607-3814 USA SN 0015-6639 J9 FOOD TECHNOL-CHICAGO JI Food Technol. PD NOV PY 2016 VL 70 IS 11 BP 70 EP 72 PG 3 WC Food Science & Technology SC Food Science & Technology GA EC5GQ UT WOS:000388161800016 ER PT J AU Nyarko, E Kniel, KE Reynnells, R East, C Handy, ET Luo, YG Millner, PD Sharma, M AF Nyarko, Esmond Kniel, Kalmia E. Reynnells, Russell East, Cheryl Handy, Eric T. Luo, Yaguang Millner, Patricia D. Sharma, Manan TI Survival and Growth of Listeria monocytogenes on Fresh-Cut "Athena" and "Rocky Ford" Cantaloupes During Storage at 4 degrees C and 10 degrees C SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article DE Listeria monocytogenes; cantaloupes; fresh-cut; temperature abuse; produce; pathogens ID UNITED-STATES; TEMPERATURE; WATERMELON; HONEYDEW; WHOLE; RIND AB Cantaloupes, marketed as "Rocky Ford," were implicated in the U.S. multistate outbreak of listeriosis in 2011, which caused multiple fatalities. Listeria monocytogenes can survive on whole cantaloupes and can be transferred to the flesh of melons. The growth of L. monocytogenes on fresh-cut "Athena" and "Rocky Ford" cantaloupe cultivars during refrigerated storage was evaluated. Fresh-cut cubes (16.4 cm(3)) from field-grown cantaloupes were each inoculated with 5 log(10) CFU/mL of a multi-strain mixture of L. monocytogenes and stored at 4 degrees C or 10 degrees C. Inoculated fresh-cut cubes were also: (1) continuously stored at 4 degrees C for 3 days; (2) temperature-abused (TA: 25 degrees C for 4 h) on day 0; or (3) stored at 4 degrees C for 24 h, exposed to TA on day 1, and subsequently stored at 4 degrees C until day 3. L. monocytogenes populations on fresh-cut melons continuously stored at 4 degrees C or 10 degrees C were enumerated on selected days for up to 15 days and after each TA event. Brix values for each cantaloupe variety were determined. L. monocytogenes populations on fresh-cut cantaloupe cubes stored at 4 degrees C increased by 1.0 and 3.0 log(10) CFU/cube by day 7 and 15, respectively, whereas those stored at 10 degrees C increased by 3.0 log(10) CFU/cube by day 7. Populations of L. monocytogenes on fresh-cut cantaloupes stored at 10 degrees C were significantly (p < 0.05) greater than those stored at 4 degrees C during the study. L. monocytogenes showed similar growth on fresh-cut "Athena" and "Rocky Ford" cubes, even though "Athena" cubes had significantly higher Brix values than the "Rocky Ford" fruit. L. monocytogenes populations on fresh-cut cantaloupes exposed to TA on day 1 and then refrigerated were significantly greater (0.74 log(10) CFU) than those stored continuously at 4 degrees C for 3 days. Storage at 10 degrees C or exposure to TA events promoted growth of L. monocytogenes on fresh-cut cantaloupe during refrigerated storage. C1 [Nyarko, Esmond; Kniel, Kalmia E.] Univ Delaware, Dept Anim & Food Sci, Newark, DE USA. [Reynnells, Russell; East, Cheryl; Handy, Eric T.; Luo, Yaguang; Millner, Patricia D.; Sharma, Manan] ARS, Environm Microbial & Food Safety Lab, USDA, Bldg 201,Room 103,10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Sharma, M (reprint author), ARS, Environm Microbial & Food Safety Lab, USDA, BARC East, Bldg 201,Room 103,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM manan.sharma@ars.usda.gov FU FDA [224-11-2046]; USDA ARS [224-11-2046, 58-1245-4-111, 8042-32420-005-00]; University of Delaware [58-1245-4-111] FX The authors are grateful to Patrick Spanninger and Dr. Gordon Johnson of the University of Delaware, Newark and Georgetown, DE; Kathryn White, Dave Clark, Bin Zhou, and Richard Stonebraker of the Environmental Microbial and Food Safety Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD; and Yi Chen, Dumitru Macarisin, Michael Mahovic, Annemarie Buchholz, and Mickey Parish of U.S. Food and Drug Administration for their assistance with this study. This study was funded by Inter-Agency Agreement 224-11-2046 between FDA and USDA ARS, Specific Cooperative Agreement 58-1245-4-111 between USDA ARS and University of Delaware, and USDA ARS Project Number 8042-32420-005-00 "Microbial Ecology and Safety of Fresh Produce." NR 20 TC 0 Z9 0 U1 6 U2 6 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 EI 1556-7125 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD NOV PY 2016 VL 13 IS 11 BP 587 EP 591 DI 10.1089/fpd.2016.2160 PG 5 WC Food Science & Technology SC Food Science & Technology GA EC5LK UT WOS:000388176600001 PM 27548768 ER PT J AU Liberatore, KL Dukowic-Schulze, S Miller, ME Chen, CB Kianian, SF AF Liberatore, Katie L. Dukowic-Schulze, Stefanie Miller, Marisa E. Chen, Changbin Kianian, Shahryar F. TI The role of mitochondria in plant development and stress tolerance SO FREE RADICAL BIOLOGY AND MEDICINE LA English DT Review DE Plant organelle; Chondriome; Mitochondrial genome; Nuclear-cytoplasmic interaction; Alloplasmic; Cytoplasmic male sterility; Stress; Development ID PROGRAMMED-CELL-DEATH; CYTOPLASMIC MALE-STERILITY; ALLOPLASMIC COMMON WHEATS; FIELD GEL-ELECTROPHORESIS; OXYGEN SPECIES PRODUCTION; ALTERNATIVE OXIDASE GENE; ABNORMAL GROWTH MUTANT; ARABIDOPSIS-THALIANA L; PLUS HYOSCYAMUS-NIGER; SHOOT APICAL MERISTEM AB Eukaryotic cells require orchestrated communication between nuclear and organellar genomes, perturbations in which are linked to stress response and disease in both animals and plants. In addition to mitochondria, which are found across eukaryotes, plant cells contain a second organelle, the plastid. Signaling both among the organelles (cytoplasmic) and between the cytoplasm and the nucleus (i.e. nuclear-cytoplasmic interactions (NCI)) is essential for proper cellular function. A deeper understanding of NCI and its impact on development, stress response, and long-term health is needed in both animal and plant systems. Here we focus on the role of plant mitochondria in development and stress response. We compare and contrast features of plant and animal mitochondrial genomes (mtDNA), particularly highlighting the large and highly dynamic nature of plant mtDNA. Plant-based tools are powerful, yet underutilized, resources for enhancing our fundamental understanding of NCI. These tools also have great potential for improving crop production. Across taxa, mitochondria are most abundant in cells that have high energy or nutrient demands as well as at key developmental time points. Although plant mitochondria act as integrators of signals involved in both development and stress response pathways, little is known about plant mtDNA diversity and its impact on these processes. In humans, there are strong correlations between particular mitotypes (and mtDNA mutations) and developmental differences (or disease). We propose that future work in plants should focus on defining mitotypes more carefully and investigating their functional implications as well as improving techniques to facilitate this research. Published by Elsevier Inc. C1 [Liberatore, Katie L.; Miller, Marisa E.; Kianian, Shahryar F.] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Liberatore, Katie L.; Kianian, Shahryar F.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Dukowic-Schulze, Stefanie; Miller, Marisa E.; Chen, Changbin] Univ Minnesota, Dept Hort Sci, St Paul, MN 55108 USA. RP Liberatore, KL (reprint author), USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. EM Katie.Liberatore@ars.usda.gov OI Dukowic-Schulze, Stefanie/0000-0003-2675-1075; Miller, Marisa/0000-0001-5599-1540 FU United States Department of Agriculture-Agricultural Research Service; National Science Foundation [IOS 1025881, IOS 1361554] FX We would like to acknowledge funding from the United States Department of Agriculture-Agricultural Research Service and from the National Science Foundation (IOS 1025881 and IOS 1361554). We also thank two anonymous reviewers for insightful comments that further improved the manuscript, and Ljudmilla Timofejeva for the EM picture. Due to the broad nature of this review, we were unable to include citations for many valuable papers in the field - we apologize to the authors whose work was not referenced. NR 336 TC 1 Z9 1 U1 18 U2 18 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0891-5849 EI 1873-4596 J9 FREE RADICAL BIO MED JI Free Radic. Biol. Med. PD NOV PY 2016 VL 100 BP 238 EP 256 DI 10.1016/j.freeradbiomed.2016.03.033 PG 19 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA EC3AH UT WOS:000387995900025 PM 27036362 ER PT J AU Ulyshen, MD Diehl, SV Jeremic, D AF Ulyshen, Michael D. Diehl, Susan V. Jeremic, Dragica TI Termites and flooding affect microbial communities in decomposing wood SO INTERNATIONAL BIODETERIORATION & BIODEGRADATION LA English DT Article DE Coarse woody debris; Decomposition; Isoptera; Saproxylic; T-RFLP ID RETICULITERMES-FLAVIPES ISOPTERA; LITTER DECOMPOSITION; MULTIVARIATE-ANALYSIS; PENINSULAR MALAYSIA; SPRUCE WOOD; DECAY-RATES; FUNGI; INSECTS; FORESTS; DIVERSITY AB Wood properties and microbial community characteristics were compared between loblolly pine (Pinus taeda L.) logs protected or unprotected from termites (Blattodea: Rhinotermitidae: Reticulitermes spp.) and other arthropods for two years in seasonally flooded and unflooded forests in the southeastern United States. Significant compositional differences were observed between treatments and between flood patterns for both bacterial and fungal communities. Bacteria were 8-9-fold more abundant in unprotected logs compared to protected logs in both flooded and unflooded forests, with the greatest abundance seen in unprotected and unflooded logs. Wood nitrogen and lignin contents were unaffected by treatment, flood pattern or levels of termite damage visible in unprotected logs. We conclude that termites alter the composition of both bacterial and fungal communities and thus have the potential to indirectly affect wood decomposition and related processes through interactions with the microbial community. Published by Elsevier Ltd. C1 [Ulyshen, Michael D.] US Forest Serv, USDA, Southern Res Stn, Athens, GA 30602 USA. [Diehl, Susan V.; Jeremic, Dragica] Mississippi State Univ, Dept Sustainable Bioprod, Starkville, MS USA. RP Ulyshen, MD (reprint author), US Forest Serv, USDA, Southern Res Stn, Athens, GA 30602 USA. EM mulyshen@fs.fed.us FU USDA Forest Service, Southern Research Station FX This research was supported by the USDA Forest Service, Southern Research Station. We thank the Noxubee National Wildlife Refuge (contact: Henry Sansing) and the John W. Starr Memorial Forest (contact: Misty Booth) for permission to carry out the study. We are also grateful to Craig Bell and Shawn Cooper (USDA FS) for their help in the field. Finally, Erica Gibson, Chanda Davis, Kayla Chaney and Julianna Stratton (MSU students) provided much-appreciated assistance in the lab. Finally, we thank Ryan Garrick (University of Mississippi), the editor and three anonymous reviewers for commenting on an early version of the manuscript. NR 52 TC 0 Z9 0 U1 15 U2 15 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0964-8305 EI 1879-0208 J9 INT BIODETER BIODEGR JI Int. Biodeterior. Biodegrad. PD NOV PY 2016 VL 115 BP 83 EP 89 DI 10.1016/j.ibiod.2016.07.017 PG 7 WC Biotechnology & Applied Microbiology; Environmental Sciences SC Biotechnology & Applied Microbiology; Environmental Sciences & Ecology GA EC3TH UT WOS:000388048700011 ER PT J AU Martin, CK Bhapkar, M Roberts, SB AF Martin, Corby K. Bhapkar, Manju Roberts, Susan B. CA CALERIE Study Grp TI Healthy Behaviors Potentially Due to Calorie Restriction Reply SO JAMA INTERNAL MEDICINE LA English DT Letter ID EMERGENCY-DEPARTMENT; BLOOD-PRESSURE; OUTCOMES C1 [Martin, Corby K.] Pennington Biomed Res Ctr, 6400 Perkins Rd, Baton Rouge, LA 70808 USA. [Bhapkar, Manju] Duke Clin Res Inst, Durham, NC USA. [Bhapkar, Manju] Duke Univ, Sch Med, Durham, NC USA. [Roberts, Susan B.] Tufts Univ, Human Nutr Res Ctr Aging, USDA, Boston, MA 02111 USA. RP Martin, CK (reprint author), Pennington Biomed Res Ctr, 6400 Perkins Rd, Baton Rouge, LA 70808 USA. EM Corby.Martin@pbrc.edu NR 5 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 330 N WABASH AVE, STE 39300, CHICAGO, IL 60611-5885 USA SN 2168-6106 EI 2168-6114 J9 JAMA INTERN MED JI JAMA Intern. Med. PD NOV PY 2016 VL 176 IS 11 BP 1724 EP 1724 DI 10.1001/jamainternmed.2016.6201 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA EC4KK UT WOS:000388097700040 PM 27820645 ER PT J AU Vereecken, H Pachepsky, Y Simmer, C Rihani, J Kunoth, A Korres, W Graf, A Franssen, HJH Thiele-Eich, I Shao, Y AF Vereecken, H. Pachepsky, Y. Simmer, C. Rihani, J. Kunoth, A. Korres, W. Graf, A. Franssen, H. J. - Hendricks Thiele-Eich, Insa Shao, Y. TI On the role of patterns in understanding the functioning of soil-vegetation-atmosphere systems SO JOURNAL OF HYDROLOGY LA English DT Review DE Patterns; Soil moisture; Upscaling; Decomposition; Wavelet analysis; Empirical orthogonal functions; Emergence; Self-organization ID EMPIRICAL MODE DECOMPOSITION; CONVECTIVE BOUNDARY-LAYER; LARGE-EDDY SIMULATION; FORECAST VERIFICATION METHODS; FOURIER-TRANSFORM ALGORITHM; REMOTELY-SENSED IMAGES; SELF-ORGANIZING MAP; TEMPORAL STABILITY; LAND-SURFACE; SPATIAL VARIABILITY AB In this paper, we review the role of patterns to improve our understanding of water, mass and energy exchange processes in soil-vegetation atmosphere systems. We explore the main mechanisms that lead to the formation of patterns in these systems and discuss different approaches to characterizing and quantifying patterns. Specific attention is given to the use of data-driven methods to detect patterns in spatial and temporal data that do not make assumptions about underlying statistical properties of patterns. These methods include e.g. decomposition methods, binning based methods, unsupervised classification and temporal stability analysis. We then analyze the value of considering patterns in evaluating model performance, reducing uncertainty in prediction of states and fluxes, as well as for upscaling and downscaling. Finally, we present ways forward to make better use of patterns in the description of flow and transport processes in soil-vegetationatmosphere systems. (C) 2016 Elsevier B.V. All rights reserved. C1 [Vereecken, H.; Graf, A.; Franssen, H. J. - Hendricks] Forschungszentrum Julich GmbH, Inst Biogeosci, Agrosphere Inst, IBG 3, Leo Brandt Str, D-52425 Julich, Germany. [Pachepsky, Y.] USDA ARS, Beltsville Agr Res Ctr, Environm Microbial & Food Safety Lab, Washington, DC 20250 USA. [Simmer, C.; Thiele-Eich, Insa] Rhein Friedrich Wilhelms Univ, Inst Meteorol, Huegel 20, D-53121 Bonn, Germany. [Rihani, J.; Kunoth, A.] Univ Cologne, Math Inst, Weyertal 86-90, D-50931 Cologne, Germany. [Korres, W.] Univ Cologne, Geograph Inst, D-50674 Cologne, Germany. [Shao, Y.] Univ Cologne, Inst Geophys & Meteorol, Pohligstr 3, D-50923 Cologne, Germany. RP Vereecken, H (reprint author), Forschungszentrum Julich GmbH, Inst Biogeosci, Agrosphere Inst, IBG 3, Leo Brandt Str, D-52425 Julich, Germany. EM h.vereecken@fz-juelich.de; Yakov.Pachepsky@ars.usda.gov; csimmer@uni-bonn.de; jrihani@uni-koeln.de; kunoth@math.uni-koeln.de; wolfgang.korres@uni-koeln.de; yshao@uni-koeln.de RI Graf, Alexander/D-1963-2009 OI Graf, Alexander/0000-0003-4870-7622 FU TR-32 Collaborative Research Center FX The authors would like to thank the TR-32 Collaborative Research Center for supporting this work. NR 303 TC 0 Z9 0 U1 17 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD NOV PY 2016 VL 542 BP 63 EP 86 DI 10.1016/j.jhydrol.2016.08.053 PG 24 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA EC6LU UT WOS:000388248400005 ER PT J AU Ghane, E Feyereisen, GW Rosen, CJ AF Ghane, Ehsan Feyereisen, Gary W. Rosen, Carl J. TI Non-linear hydraulic properties of woodchips necessary to design denitrification beds SO JOURNAL OF HYDROLOGY LA English DT Article DE Denitrifying bioreactor; Hydraulic conductivity; Hydraulic retention time; Temperature; Tile drainage; Woodchip bioreactor ID NON-DARCY FLOW; POROUS-MEDIA; AGRICULTURAL DRAINAGE; DENITRIFYING BIOREACTORS; MICROCYSTIS-AERUGINOSA; PENETRATING WELL; CONFINED AQUIFER; NITRATE REMOVAL; PERFORMANCE; TEMPERATURE AB Denitrification beds are being used to reduce the transport of water-soluble nitrate via subsurface drainage systems to surface water. Only recently has the non-linearity of water flow through woodchips been ascertained. To successfully design and model denitrification beds with optimum nitrate removal, a better understanding of flow in denitrification beds is needed. The main objectives of this study were to characterize the hydraulic properties of old degraded woodchips and provide a better understanding of the factors affecting flow. To achieve this goal, we conducted constant-head column experiments using old woodchips that were excavated from a four-year old denitrification bed near Willmar, Minnesota, USA. For Izbash's equation, the non-Darcy exponent (n) ranged from 0.76 to 0.87 that indicates post-linear regime, and the permeability coefficient (M-10) at 10 degrees C ranged from 0.9 to 2.6 cm s(-1). For Forchheimer's equation, the intrinsic permeability of 5.6 x 10(-5) cm(2) and omega constant of 0.40 (at drainable porosity of 0.41) closely resembled the in-situ properties found in a previous study. Forchheimer's equation was better than that of Izbash's for describing water flow through old woodchips, and the coefficients of the former provided stronger correlations with drainable porosity. The strong correlation between intrinsic permeability and drainable porosity showed that woodchip compaction is an important factor affecting water flow through woodchips. Furthermore, we demonstrated the importance of temperature effects on woodchip hydraulics. In conclusion, the hydraulic properties of old woodchips should be characterized using a non-Darcy equation to help design efficient systems with optimum nitrate removal. (C) 2016 Elsevier B.V. All rights reserved. C1 [Ghane, Ehsan; Rosen, Carl J.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Feyereisen, Gary W.] USDA ARS, Soil & Water Management Res Unit, St Paul, MN 55108 USA. RP Ghane, E (reprint author), Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. EM ehsan.ghane@outlook.com FU Minnesota Discovery, Research and Innovation Economy; Minnesota Soybean Research and Promotion Council; Minnesota Corn Research and Promotion Council; Minnesota Agricultural Water Resource Center; Minnesota Turkey Research and Promotion Council FX Funding of this research was provided in part by Minnesota Discovery, Research and Innovation Economy; Minnesota Soybean Research and Promotion Council; Minnesota Corn Research and Promotion Council; Minnesota Agricultural Water Resource Center, and Minnesota Turkey Research and Promotion Council. The funding sources were not involved in the study design, collection, analysis, interpretation of data, or preparation of the manuscript. NR 55 TC 0 Z9 0 U1 9 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD NOV PY 2016 VL 542 BP 463 EP 473 DI 10.1016/j.jhydrol.2016.09.021 PG 11 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA EC6LU UT WOS:000388248400033 ER PT J AU Estep, AS Becnel, JJ Lee, ST AF Estep, Alden S. Becnel, James J. Lee, Stephen T. TI Toxicity of Compounds Isolated from White Snakeroot (Ageratina altissima) to Adult and Larval Yellow Fever Mosquitoes (Aedes aegypti) SO NATURAL PRODUCT COMMUNICATIONS LA English DT Article DE Aedes aegypti; Ageratina altissima; Chromene; Benzofurans; Natural pesticide ID EUPATORIUM URTICAEFOLIUM; INSECTICIDAL CHROMENES; PESTICIDES; ASTERACEAE; KETONES; ANALOGS AB Botanicals have been the source for several classes of pesticides for mosquitoes. However, increasing resistance to these products and reduced numbers of choices necessitate the search for new active ingredients. Ageratina altissima (L.) King & H. Rob. or white snakeroot, is found throughout the eastern United States and contains known toxic compounds. We examine chromenes (benzopyrans) and benzofurans isolated from this plant for larvicidal and adulticidal activity against the dengue vector Aedes aegypti L. Initial activity identified several compounds that were effective against either larvae or adults. Interestingly, only two compounds were effective against both larva and adults. Dose curves were constructed from further testing of these active compounds to allow comparative ranking of efficacy. We identified dehydrotremetone as the most effective larvicide (0.03 +/- 0.001 ng/mu L) and 6-acetyl-7-methoxy-2,2-dimethylchromene as the most effective adulticide (1.17 +/- 0.31 mu g/org) although other compounds were also active. This study provides additional useful data for evaluation of chromenes and benzofurans as possible mosquiticidal agents. C1 [Estep, Alden S.; Becnel, James J.] ARS, Ctr Med Agr & Vet Entomol, USDA, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. [Estep, Alden S.] CMAVE Detachment, Navy Entomol Ctr Excellence, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. [Lee, Stephen T.] ARS, Poisonous Plant Res Lab, USDA, 1150 East 1400 North, Logan, UT 84341 USA. RP Estep, AS (reprint author), ARS, Ctr Med Agr & Vet Entomol, USDA, 1700 SW 23rd Dr, Gainesville, FL 32608 USA.; Estep, AS (reprint author), CMAVE Detachment, Navy Entomol Ctr Excellence, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. EM alden.estep@ars.usda.gov FU Deployed Warfighters Protection (DWFP) Program of the Armed Forces Pest Management Board FX The authors would like to acknowledge the technical assistance of Jessica Louton and funding support from the Deployed Warfighters Protection (DWFP) Program of the Armed Forces Pest Management Board. We would also like to thank Dr. Nurhayat Tabanca, Dr. Charles Cantrell, and Neil Sanscrainte for internal review of the manuscript and copy edits. NR 18 TC 0 Z9 0 U1 3 U2 3 PU NATURAL PRODUCTS INC PI WESTERVILLE PA 7963 ANDERSON PARK LN, WESTERVILLE, OH 43081 USA SN 1934-578X EI 1555-9475 J9 NAT PROD COMMUN JI Nat. Prod. Commun. PD NOV PY 2016 VL 11 IS 11 BP 1675 EP 1677 PG 3 WC Chemistry, Medicinal; Food Science & Technology SC Pharmacology & Pharmacy; Food Science & Technology GA EC6MU UT WOS:000388251200013 ER PT J AU Schrader, KK Cantrell, CL Midiwo, JO Muhammad, I AF Schrader, Kevin K. Cantrell, Charles L. Midiwo, Jacob O. Muhammad, Ilias TI Compounds from Terminalli brownii Extracts with Toxicity against the Fish Pathogenic Bacterium Flavobacterium columnare SO NATURAL PRODUCT COMMUNICATIONS LA English DT Article DE Antibacterial; Channel catfish; Columnaris; Ellagic acid; Flavobacterium columnare; Ictalurus punctatus; Tannins; Terminalli brownii AB The pond-raised channel catfish (Ictalurus punctatus) industry in the United States of America can incur losses of Over a $100 million annually due to bacterial diseases including columnaris disease caused by Flavobacterium columnare. One management approach available to catfish producers is the use of medicated-feed containing antibiotics. However, the negative attributes of antibiotic use in agriculture include public concerns and the potential development of antibiotic-resistant bacteria. Therefore, the discovery of environmentally-safe natural compounds for use as therapeutic agents would greatly benefit the catfish industry. In this study, a rapid bioassay was used to evaluate crude plant extracts as the first step in the discovery of natural therapeutants. Plant extracts from Terminalia brownii were found to be inhibitory towards F. columnare. The minimum inhibitory concentration (MIC) of the 5% water-methanol extract of T. brownii (stem bark) was 10 mu g/mL and the 24 h 50% inhibition concentration (IC50) was 40 mu g/mL. Subsequent bioassay-guided fractionation of the T. brownii ethanol extract using reverse phase C-4 chromatography revealed the highest level of activity in the aqueous:methanol (50:50) fraction. HPLC analysis and subsequent purification of this fraction provided two compounds identified as ellagic acid (1) and 4-O-(3 '',4 ''-di-O-galloyl-alpha-L-rhamnopyranosyl)ellagic acid (2). Compound 2 was the most active isolated compound, with a minimum inhibitory concentration (MIC) of 10 +/- 0 mu g/mL and 24 h IC50 of 31 +/- 1 mu g/mL. Although 1 was more active according to a MIC of 6 +/- 5 mu g/mL, its 24 h IC50 was >100 mu g/mL, and, therefore, it was less active overall of the two most active isolated compounds. C1 [Schrader, Kevin K.; Cantrell, Charles L.] ARS, USDA, Nat Prod Utilizat Res Unit, Natl Ctr Nat Prod Res, POB 1848, University, MS 38677 USA. [Midiwo, Jacob O.] Univ Nairobi, Dept Chem, POB 30197-00100, Nairobi, Kenya. [Muhammad, Ilias] Univ Mississippi, Sch Pharm, Natl Ctr Nat Prod Res, University, MS 38677 USA. RP Schrader, KK (reprint author), ARS, USDA, Nat Prod Utilizat Res Unit, Natl Ctr Nat Prod Res, POB 1848, University, MS 38677 USA. EM kevin.schrader@ars.usda.gov NR 11 TC 0 Z9 0 U1 1 U2 1 PU NATURAL PRODUCTS INC PI WESTERVILLE PA 7963 ANDERSON PARK LN, WESTERVILLE, OH 43081 USA SN 1934-578X EI 1555-9475 J9 NAT PROD COMMUN JI Nat. Prod. Commun. PD NOV PY 2016 VL 11 IS 11 BP 1679 EP 1682 PG 4 WC Chemistry, Medicinal; Food Science & Technology SC Pharmacology & Pharmacy; Food Science & Technology GA EC6MU UT WOS:000388251200014 ER PT J AU Blythe, EK Tabanca, N Demirci, B Tsikolia, M Bloomquist, JR Bernier, UR AF Blythe, Eugene K. Tabanca, Nurhayat Demirci, Betul Tsikolia, Maia Bloomquist, Jeffrey R. Bernier, Ulrich R. TI Lantana montevidensis Essential Oil: Chemical Composition and Mosquito Repellent Activity against Aedes aegypti SO NATURAL PRODUCT COMMUNICATIONS LA English DT Article DE Lantana montevidensis; Trailing lantana; Essential oils; beta-Elemene; Mosquito repellent; Aedes aegypti ID GERMACRENE-D; CAMARA L.; LEAVES AB The essential oil (EO) of Lantana montevidensis (Spreng.) Briq. (L. sellowiana Link & Otto) was investigated for its chemical composition and mosquito repellent activity. The essential oil obtained by hydrodistillation of aerial plant parts was analyzed by GC-FID and GC-MS. The major constituents were beta-elemene (22.0%), beta-caryophyllene (20.1%), and germacrene D (9.4%). Sesquiterpene hydrocarbons were present in considerable quantities (78.9%) in the L. montevidensis EO, followed by oxygenated sesquiterpenes (8.9%), monoterpene hydrocarbons (7.7%), oxygenated monoterpenes (1.9%), diterpenes (1.2%) and other compounds (0.2%). The oil of L. montevidensis was repellent with a minimum effective dosage (MED) of 0.021 +/- 0.013 mg/cm(2) as compared with that of the positive control N,N-diethyl-3-methylbenzamide (DEET) with a MED of 0.006 +/- 0.001 mg/cm(2)) against Aedes aegypti L. The major compound beta-elemene was tested individually for its repellency and had a MED value of 0.23 +/- 0.14 mg/cm(2) (DEET was 0.008 +/- 0.001 mg/cm(2)). This is the first report on the repellent activity of L. montevidensis EO and beta-elemene using human-based in vivo assays against Ae. aegypti. C1 [Blythe, Eugene K.] Mississippi State Univ, South Mississippi Branch Expt Stn, Coastal Res & Extens Ctr, Poplarville, MS 39470 USA. [Tabanca, Nurhayat; Tsikolia, Maia; Bloomquist, Jeffrey R.] Univ Florida, Emerging Pathogens Inst, Dept Entomol & Nematol, Gainesville, FL 32610 USA. [Demirci, Betul] Anadolu Univ, Fac Pharm, Dept Pharmacognosy, TR-26470 Eskisehir, Turkey. [Tabanca, Nurhayat; Tsikolia, Maia; Bernier, Ulrich R.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Blythe, EK (reprint author), Mississippi State Univ, South Mississippi Branch Expt Stn, Coastal Res & Extens Ctr, Poplarville, MS 39470 USA.; Tabanca, N (reprint author), Univ Florida, Emerging Pathogens Inst, Dept Entomol & Nematol, Gainesville, FL 32610 USA.; Tabanca, N; Bernier, UR (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. EM gene.blythe@msstate.edu; ntabanca@ufl.edu; uli.bernier@ars.usda.gov FU Special Research Initiative from the Mississippi Agricultural and Forestry Experiment Station; National Institute of Food and Agriculture, U.S. Department of Agriculture [MIS-219060]; Deployed War-Fighter Protection Research Program - U.S. Department of Defense through the Armed Forces Pest Management Board FX This study was supported in part by (i) a Special Research Initiative grant from the Mississippi Agricultural and Forestry Experiment Station, (ii) a grant from the National Institute of Food and Agriculture, U.S. Department of Agriculture, Hatch project MIS-219060, and (iii) the Deployed War-Fighter Protection Research Program Grant funded by the U.S. Department of Defense through the Armed Forces Pest Management Board. We thank Natasha M. Agramonte and Greg Allen (USDA, ARS, Center for Medical, Agricultural, and Veterinary Entomology, Gainesville, FL) for performing the mosquito repellent bioassays. NR 39 TC 0 Z9 0 U1 1 U2 1 PU NATURAL PRODUCTS INC PI WESTERVILLE PA 7963 ANDERSON PARK LN, WESTERVILLE, OH 43081 USA SN 1934-578X EI 1555-9475 J9 NAT PROD COMMUN JI Nat. Prod. Commun. PD NOV PY 2016 VL 11 IS 11 BP 1713 EP 1716 PG 4 WC Chemistry, Medicinal; Food Science & Technology SC Pharmacology & Pharmacy; Food Science & Technology GA EC6MU UT WOS:000388251200022 ER PT J AU Cooley, RA Wolter, PT Sturtevant, BR AF Cooley, Rayma A. Wolter, Peter T. Sturtevant, Brian R. TI Quantifying Early-Seral Forest Composition with Remote Sensing SO PHOTOGRAMMETRIC ENGINEERING AND REMOTE SENSING LA English DT Article ID SOUTHERN-BOREAL FOREST; LANDSAT TIME-SERIES; FIRE SEVERITY; BURN SEVERITY; NORTHERN MINNESOTA; CLIMATE-CHANGE; CANADA; REGENERATION; LANDSCAPE; COVER AB Spatially explicit modeling of recovering forest structure within two years following wildfire disturbance has not been attempted, yet such knowledge is critical for determining successional pathways. We used remote sensing and field data, along with digital climate and terrain data, to model and map early-seral aspen structure and vegetation species richness following wildfire. Richness was the strongest model (RMSE = 2.47 species, Adj. R-2 = 0.60), followed by aspen stem diameter, basal area (BA), height, density, and percent cover (Adj. R-2 range = 0.22 to 0.53). Effects of pre-fire aspen BA and fire severity on post-fire aspen structure and richness were analyzed. Post-fire recovery attributes were not significantly related to fire severity, while all but percent cover and richness were sensitive to pre-fire aspen BA (Adj. R-2 range = 0.12 to 0.33, p < 0.001). This remote mapping capability will enable improved prediction of future forest composition and structure, and associated carbon stocks. C1 [Cooley, Rayma A.] US Forest Serv, Six Rivers Natl Forest, 741 State Hwy 36, Bridgeville, CA 95526 USA. [Cooley, Rayma A.; Wolter, Peter T.] Iowa State Univ, 339 Sci Hall 2, Ames, IA 50011 USA. [Sturtevant, Brian R.] US Forest Serv, Northern Res Stn, 5985 Highway, Rhinelander, WI 54501 USA. RP Cooley, RA (reprint author), US Forest Serv, Six Rivers Natl Forest, 741 State Hwy 36, Bridgeville, CA 95526 USA. EM raymacooley@fs.fed.us FU National Science Foundation (RAPID Collaborative Research Grant) [1201484]; USDA Agriculture & Food Research Initiative (McIntire Stennis project) USFS National Fire Plan [IOW5348] FX This project was funded by the National Science Foundation (RAPID Collaborative Research Grant 1201484) and the USDA Agriculture & Food Research Initiative (McIntire Stennis project: IOW5348) USFS National Fire Plan. The authors would like to thank Superior National Forest for providing research access to the Boundary Waters Canoe Area Wilderness; Lisa Schulte Moore (Iowa State University, Ames, IA), Jonathan Chipman (Dartmouth, Hanover, NH), Eric Gustafson and John Stanovick (USFS, Newtown Square, PA), and Shawn Fraver (University of Maine, Orono, ME) for reviewing earlier drafts of the manuscript; Jared Niemi and Lendie Follett (Iowa State University, Ames, IA) for statistical consulting; and Louis Hilgemann (VI Dept. of Agriculture, Kingshill, VI), Bert and Johnna Hyde (Ely, MN), and Lawson Gerdes (Minnesota DNR, St. Paul, MN) for field assistance and guidance. We also wish to thank the three blind reviewers, whose comments greatly improved the quality of this manuscript. NR 56 TC 0 Z9 0 U1 5 U2 5 PU AMER SOC PHOTOGRAMMETRY PI BETHESDA PA 5410 GROSVENOR LANE SUITE 210, BETHESDA, MD 20814-2160 USA SN 0099-1112 EI 2374-8079 J9 PHOTOGRAMM ENG REM S JI Photogramm. Eng. Remote Sens. PD NOV PY 2016 VL 82 IS 11 BP 853 EP 863 DI 10.14358/PERS.82.11.853 PG 11 WC Geography, Physical; Geosciences, Multidisciplinary; Remote Sensing; Imaging Science & Photographic Technology SC Physical Geography; Geology; Remote Sensing; Imaging Science & Photographic Technology GA EC9QV UT WOS:000388480100006 ER PT J AU Dunn, E Hough-Goldstein, J Hanks, LM Millar, JG D'Amico, V AF Dunn, E. Hough-Goldstein, J. Hanks, L. M. Millar, J. G. D'Amico, V. TI Range of Attraction of Pheromone Lures and Dispersal Behavior of Cerambycid Beetles SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE Cerambycidae; movement; forest fragmentation; Xylotrechus colonus; Prionus laticollis ID HOST-PLANT VOLATILES; COLEOPTERA CERAMBYCIDAE; SIMULTANEOUSLY ATTRACT; PANEL TRAPS; ETHANOL; PHENOLOGY; PINE; COMPONENTS; EFFICACY; INSECTS AB Cerambycid beetles (Coleoptera: Cerambycidae) can locate suitable hosts and mates by sensing pheromones and plant volatiles. Many cerambycid pheromone components have been identified and are now produced synthetically for trap lures. The range over which these lures attract cerambycids within a forest, and the tendency for cerambycids to move out of a forest in response to lures, have not been explored previously. We conducted two field experiments using baited and unbaited panel traps in northern Delaware to investigate these questions. Within forest fragments, unbaited traps that were 2 m from a baited trap captured more beetles than unbaited control traps, suggesting increased cerambycid activity leading to more by-catch in unbaited traps at 2 m from the pheromone source. Traps at further distances from a baited trap did not catch significantly more beetles than equivalent controls. In contrast, male Prionus laticollis (Drury), which were attracted by the likely female-produced sex pheromone 3,5-dimethyldodecanoic acid, were rarely collected in unbaited traps at any distance from baited traps. Outside the forest, baited traps attracted significantly more cerambycids than unbaited traps at distances up to 40 m from the forest edge, with catch generally decreasing between 8 and 40 m from the forest. Some cerambycids were collected in both baited and unbaited traps at all distances from the forest edge, indicating that at least some species dispersed out of the forest independent of any pheromonal attractants. Our results provide context to previous studies that used these pheromone lures, and offer insights into cerambycid dispersal behavior. C1 [Dunn, E.; Hough-Goldstein, J.] Univ Delaware, Dept Entomol & Wildlife Ecol, 531 S Coll Ave, Newark, DE 19716 USA. [Hanks, L. M.] Univ Illinois, Dept Entomol, 505 S Goodwin Ave MC-118, Urbana, IL 61801 USA. [Millar, J. G.] Univ Calif Riverside, Dept Entomol, 900 Univ Ave, Riverside, CA 92521 USA. [D'Amico, V.] US Forest Serv, Northern Res Stn, 531 S Coll Ave, Newark, DE 19716 USA. RP Dunn, E (reprint author), Univ Delaware, Dept Entomol & Wildlife Ecol, 531 S Coll Ave, Newark, DE 19716 USA. EM edunn@udel.edu; jhough@udel.edu; hanks@life.illinois.edu; jocelyn.millar@ucr.edu; vincedamico@gmail.com FU USDA Forest Service Northern Research Station; USDA-APHIS grant [14-8130-1422-CA]; Alphawood Foundation of Chicago FX Thanks to Aurora Madison and Kaitlin Rim for assistance in the field and laboratory. We also thank Judith Mongold-Diers (University of Illinois) for assistance in preparing pheromone lures. Funding was provided by the USDA Forest Service Northern Research Station (to V.D.A.), USDA-APHIS grant 14-8130-1422-CA (to J.G.M. and L.M.H.), and the Alphawood Foundation of Chicago (to L.M.H.). NR 46 TC 0 Z9 0 U1 4 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD NOV PY 2016 VL 109 IS 6 BP 872 EP 880 DI 10.1093/aesa/saw055 PG 9 WC Entomology SC Entomology GA EC4GV UT WOS:000388086100006 ER PT J AU Li, ZG Huang, SK Huang, WF Geng, HY Zhao, YZ Li, M Chen, YP Su, SK AF Li, Zhiguo Huang, Shaokang Huang, Wei-fone Geng, Haiyang Zhao, Yazhou Li, Meng Chen, Yanping Su, Songkun TI A scientific note on detection of honeybee viruses in the darkling beetle (Alphitobius diaperinus, Coleoptera: Tenebrionidae), a new pest in Apis cerana cerana colonies SO APIDOLOGIE LA English DT Article DE viruses; Apis cerana; Alphitobius diaperinus; honeybees ID DEFORMED-WING VIRUS; MELLIFERA C1 [Li, Zhiguo; Huang, Shaokang; Huang, Wei-fone; Geng, Haiyang; Li, Meng; Su, Songkun] Fujian Agr & Forestry Univ, Coll Bee Sci, Fuzhou 350002, Peoples R China. [Zhao, Yazhou] Chinese Acad Agr Sci, Inst Apiculture, Beijing 100093, Peoples R China. [Chen, Yanping] USDA ARS, Bee Res Lab, Beltsville, MD 20705 USA. RP Su, SK (reprint author), Fujian Agr & Forestry Univ, Coll Bee Sci, Fuzhou 350002, Peoples R China. EM susongkun@zju.edu.cn FU earmarked fund for Modern Agro-industry Technology Research System [CARS-45-KXJ3]; National Natural Science Foundation of China [31340061] FX This work was supported by the earmarked fund for Modern Agro-industry Technology Research System (No. CARS-45-KXJ3) and National Natural Science Foundation of China (31340061). NR 9 TC 0 Z9 0 U1 6 U2 6 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 0044-8435 EI 1297-9678 J9 APIDOLOGIE JI Apidologie PD NOV PY 2016 VL 47 IS 6 BP 759 EP 761 DI 10.1007/s13592-016-0430-1 PG 3 WC Entomology SC Entomology GA EB9CW UT WOS:000387690500004 ER PT J AU Villa, JD Danka, RG Harris, JW AF Villa, Jose D. Danka, Robert G. Harris, Jeffrey W. TI Selecting honeybees for worker brood that reduces the reproduction of Varroa destructor SO APIDOLOGIE LA English DT Article DE Varroa destructor; selection; honeybee; reproduction; heritability ID BEES APIS-MELLIFERA; HYMENOPTERA APIDAE; JACOBSONI MESOSTIGMATA; MITE REPRODUCTION; RESISTANCE; HOST; COLONIES; HERITABILITY; BEHAVIOR; CERANA AB We investigated an effect of Apis mellifera worker brood on the reproduction of Varroa destructor as a resistance trait by conducting seven generations of bidirectional selection. Initial tests showed twofold differences in mite fecundity (progeny per foundress mites) between colonies of different origins. In the first two generations of selection, low and high lines differed significantly in mite fecundity (2.6 vs. 2.9 and 2.3 vs. 2.8), but in five subsequent generations the average values of the low fecundity lines (ranges of 2.7 to 3.3) became indistinguishable from high lines (2.9 to 3.1). Similarly, the relationship between mite fecundity in 117 offspring colonies and in their 26 queen and 22 drone parent colonies was partly significant in the first three generations but not in subsequent generations. These findings suggest that adaptation of mites to host cues, loss of resistance alleles in a small breeding population, or environmental effects present challenges to breeding for this trait. C1 [Villa, Jose D.; Danka, Robert G.] ARS, USDA, Honey Bee Breeding Genet & Physiol Lab, 1157 Ben Hur Rd, Baton Rouge, LA 70820 USA. [Harris, Jeffrey W.] Box 9655, Mississippi State, MS 39762 USA. RP Danka, RG (reprint author), ARS, USDA, Honey Bee Breeding Genet & Physiol Lab, 1157 Ben Hur Rd, Baton Rouge, LA 70820 USA. EM bob.danka@ars.usda.gov NR 43 TC 0 Z9 0 U1 13 U2 13 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 0044-8435 EI 1297-9678 J9 APIDOLOGIE JI Apidologie PD NOV PY 2016 VL 47 IS 6 BP 771 EP 778 DI 10.1007/s13592-016-0433-y PG 8 WC Entomology SC Entomology GA EB9CW UT WOS:000387690500006 ER PT J AU Steinhauser, CB Landers, M Myatt, L Burghardt, RC Vallet, JL Bazer, FW Johnson, GA AF Steinhauser, Chelsie B. Landers, McKinsey Myatt, Louise Burghardt, Robert C. Vallet, Jeffrey L. Bazer, Fuller W. Johnson, Greg A. TI Fructose Synthesis and Transport at the Uterine-Placental Interface of Pigs: Cell-Specific Localization of SLC2A5, SLC2A8, and Components of the Polyol Pathway SO BIOLOGY OF REPRODUCTION LA English DT Article DE endometrium; fructose; pig; placenta; polyol pathway; SLC2A5; SLC2A8 ID GLUCOSE TRANSPORTERS; OVINE UTERUS; FETAL PIG; PERIIMPLANTATION CONCEPTUSES; SELECT NUTRIENTS; GENE-EXPRESSION; METABOLISM; PREGNANCY; PROGESTERONE; LACTATE AB The fetal fluids and uterine flushings of pigs contain higher concentrations of fructose than glucose, but fructose is not detected in maternal blood. Fructose can be synthesized from glucose via enzymes of the polyol pathway, aldose reductase (AKR1B1) and sorbitol dehydrogenase (SORD), transported across cell membranes by solute carriers SLC2A5 and SLC2A8, and converted to fructose-1-phosphate by ketohexokinase (KHK). SLC2A8, SLC2A5, AKR1B1, SORD, and KHK mRNAs and proteins were analyzed using quantitative PCR and immunohistochemistry or in situ hybridization in endometria and placentae of cyclic and pregnant gilts, cyclic gilts injected with estrogen, and ovariectomized gilts injected with progesterone. Progesterone up-regulated SLC2A8 protein in uterine luminal (LE) and glandular epithelia during the peri-implantation period, and expression became exclusively placental, chorion and blood vessels, after Day 30. P4 up-regulated SLC2A5 mRNA in uterine LE and glandular epithelia after implantation, and the chorion expressed SLC2A5 between Days 30 and 85. AKR1B1 and SORD proteins localized to uterine LE during the peri-implantation period, but expression switched to chorion by Day 20 and was maintained through Day 85. Uterine expression of AKR1B1 mRNA was down-regulated by estrogen. KHK protein localized to trophectoderm/chorion throughout gestation. These results provide evidence that components for the conversion of glucose to fructose and for fructose transport are present at the uterine-placental interface of pigs. The shift in expression from LE to chorion during pregnancy suggests free-floating conceptuses are supported by fructose synthesized by the uterus, but after implantation, the chorion becomes self-sufficient for fructose synthesis and transport. C1 [Steinhauser, Chelsie B.; Landers, McKinsey; Myatt, Louise; Burghardt, Robert C.; Johnson, Greg A.] Texas A&M Univ, Dept Vet Integrat Biosci, College Stn, TX 77843 USA. [Bazer, Fuller W.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA. [Vallet, Jeffrey L.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. RP Johnson, GA (reprint author), Texas A&M Univ, Dept Vet Integrat Biosci, College Stn, TX 77843 USA. EM gjohnson@cvm.tamu.edu FU National Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2006-35203-17199]; Office of Graduate Studies at Texas AM University; Texas A&M College of Veterinary Medicine Graduate Research Mini-Grant Award; Graduate Diversity Fellowship FX This project was supported by National Research Initiative Competitive Grant no. 2006-35203-17199 from the USDA National Institute of Food and Agriculture to G.A.J., by a Graduate Diversity Fellowship provided to C.B.S. by the Office of Graduate Studies at Texas A&M University, and by a Texas A&M College of Veterinary Medicine Graduate Research Mini-Grant Award to C.B.S. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 51 TC 0 Z9 0 U1 3 U2 3 PU SOC STUDY REPRODUCTION PI MADISON PA 1691 MONROE ST,SUITE # 3, MADISON, WI 53711-2021 USA SN 0006-3363 EI 1529-7268 J9 BIOL REPROD JI Biol. Reprod. PD NOV 1 PY 2016 VL 95 IS 5 AR 108 DI 10.1095/biolreprod.116.142174 PG 14 WC Reproductive Biology SC Reproductive Biology GA EC1HU UT WOS:000387856300016 PM 27535960 ER PT J AU Iskandar, AR Miao, BC Li, XL Hu, KQ Liu, C Wang, XD AF Iskandar, Anita R. Miao, Benchun Li, Xinli Hu, Kang-Quan Liu, Chun Wang, Xiang-Dong TI beta-Cryptoxanthin Reduced Lung Tumor Multiplicity and Inhibited Lung Cancer Cell Motility by Downregulating Nicotinic Acetylcholine Receptor alpha 7 Signaling SO CANCER PREVENTION RESEARCH LA English DT Article ID A/J MOUSE MODEL; EPITHELIAL-CELLS; RETINOIC ACID; ALPHA-7-NICOTINIC RECEPTOR; LYCOPENE SUPPLEMENTATION; SQUAMOUS METAPLASIA; RISK; TUMORIGENESIS; EXPRESSION; FERRETS AB Despite the consistent association between a higher intake of the provitamin A carotenoid beta-cryptoxanthin (BCX) and a lower risk of lung cancer among smokers, potential mechanisms supporting BCX as a chemopreventive agent are needed. We first examined the effects of BCX on 4-[methyl nitrosamino]-1-[3-pyridyl]- 1-butanone (NNK)-induced lung tumorigenesis in A/J mice. BCX supplementation was given daily to the mice starting 2 weeks prior to the injection of NNK and continued 16 weeks after NNK injection. BCX supplementation resulted in a dose-dependent increase of BCX concentration in both serum and lungs of the mice without a significant alteration of vitamin A (retinol and retinyl palmitate) concentration. BCX significantly reduced the multiplicity of the NNK-induced lung tumor by 52% to 63% compared with the NNK-treated mice without BCX supplementation. The protective effect of BCX in the lungs was associated with reductions of both mRNA and protein of the homopentameric neuronal nicotinic acetylcholine receptor alpha 7 (alpha 7-nAChR), which has been implicated in lung tumorigenesis. We then conducted an in vitro cell culture study and found that BCX treatment suppressed alpha 7-nAChR expression and inhibited the migration and invasion of alpha 7-nAChR-positive lung cancer cells but not in cells lacking alpha 7-nAChR. The activities of BCX were significantly attenuated by activators of alpha 7-nAChR/PI3K signaling or by overexpression of constitutively active PI3K. Collectively, the results suggest that BCX inhibits lung tumorigenesis and cancer cell motility through the downregulation of alpha 7-nAChR/PI3K signaling, independent of its provitamin A activity. Therefore, BCX can be used as a chemopreventive agent or a chemotherapeutic compound against lung cancer. (C) 2016 AACR. C1 [Iskandar, Anita R.; Miao, Benchun; Li, Xinli; Hu, Kang-Quan; Liu, Chun; Wang, Xiang-Dong] Tufts Univ, Nutr & Canc Biol Lab, Jean Mayer United States Dept Agr Human Nutr, Res Ctr Aging, Boston, MA 02111 USA. RP Wang, XD (reprint author), Tufts Univ, Gerald J & Dorothy R Friedman Sch Nutr Sci & Poli, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St,Room 514, Boston, MA 02111 USA. EM xiang-dong.wang@tufts.edu FU NCI/NIH grant [R21CA176256]; USDA/ARS grant [1950-51000-074S] FX This study was financially supported by the NCI/NIH grant R21CA176256 (to X.-D. Wang) and USDA/ARS grant 1950-51000-074S (to X.-D. Wang). NR 49 TC 0 Z9 0 U1 3 U2 3 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 1940-6207 EI 1940-6215 J9 CANCER PREV RES JI Cancer Prev. Res. PD NOV PY 2016 VL 9 IS 11 BP 875 EP 886 DI 10.1158/1940-6207.CAPR-16-0161 PG 12 WC Oncology SC Oncology GA EB8ZZ UT WOS:000387682200007 PM 27623933 ER PT J AU Cao, YZ Tian, NN Bahr, D Zavattieri, PD Youngblood, J Moon, RJ Weiss, J AF Cao, Yizheng Tian, Nannan Bahr, David Zavattieri, Pablo D. Youngblood, Jeffrey Moon, Robert J. Weiss, Jason TI The influence of cellulose nanocrystals on the microstructure of cement paste SO CEMENT & CONCRETE COMPOSITES LA English DT Article DE Cellulose nanocrystal; Cement; Agglomeration; Ultrasonication; Flexural strength; Nanoindentation ID PERFORMANCE; CONCRETE AB This paper reports the influence of raw and sonicated cellulose nanocrystals (CNCs) on the microstructure of cement paste. A novel centrifugation method is designed to measure the concentrations of the adsorbed CNCs (aCNCs) on the cement surface, and the free CNCs (fCNCs) which are mobile in water. It is found that, the majority of the CNCs (>94%) are aCNCs. More importantly, sonication does not significantly reduce the amount of aCNCs (reduction of less than 2%). We surmise that, after sonication, the aCNCs are primarily dispersed over the cement surface, instead of becoming fCNCs via sonication. Isothermal calorimetry and energy-dispersive X-ray spectroscopy (EDX) results support this theory. The water desorption tests show that the total porosities of cement pastes with raw and sonicated CNCs are 14.8% and 14.4%, which showed a reduction from 16% for the plain cement paste. The porosity reduction is a result of an increase in the degree of hydration. The advantage of sonicated CNCs is they are dispersed, avoiding therefore agglomerates that can lead to pores, voids, and air entrapment. The nanoindentation results show that the reduced indentation modulus on the interfacial regions between cement particles and the low density CSH is increased when CNCs are used. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Cao, Yizheng] Albany Mol Res Inc, SSCI, W Lafayette, IN 47906 USA. [Tian, Nannan; Bahr, David; Youngblood, Jeffrey] Purdue Univ, Sch Mat Engn, W Lafayette, IN 47907 USA. [Zavattieri, Pablo D.] Purdue Univ, Lyles Sch Civil Engn, W Lafayette, IN 47907 USA. [Moon, Robert J.] US Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. [Weiss, Jason] Oregon State Univ, Sch Civil & Construct Engn, Corvallis, OR 97331 USA. RP Weiss, J (reprint author), Oregon State Univ, Sch Civil & Construct Engn, Corvallis, OR 97331 USA. EM jason.weiss@oregonstate.edu RI Bahr, David/A-6521-2012 OI Bahr, David/0000-0003-2893-967X FU National Science Foundation [1131596] FX This work was supported by the National Science Foundation through Awards no. CMMI #1131596. The authors are grateful for the USFS Forest Products Laboratory for providing the CNC materials. NR 27 TC 0 Z9 0 U1 6 U2 6 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0958-9465 EI 1873-393X J9 CEMENT CONCRETE COMP JI Cem. Concr. Compos. PD NOV PY 2016 VL 74 BP 164 EP 173 DI 10.1016/j.cemconcomp.2016.09.008 PG 10 WC Construction & Building Technology; Materials Science, Composites SC Construction & Building Technology; Materials Science GA EB8GU UT WOS:000387630300015 ER PT J AU Graybosch, RA Ohm, JB Dykes, L AF Graybosch, R. A. Ohm, Jae-Bom Dykes, Linda TI Observations on the Quality Characteristics of Waxy (Amylose-Free) Winter Wheats SO CEREAL CHEMISTRY LA English DT Article ID FLOURS; STARCH; GLUTEN; BLENDS; BREAD AB Previous investigations have suggested waxy (amylose-free) wheats (Triticum aestivum L.) possess weak gluten properties and may not be suitable for commercial gluten extraction. This limitation could prevent the use of waxy wheat as a source of unique starch, because gluten is a by-product of the wheat starch purification process. Fifty waxy wheat lines were used to determine the extent to which gluten protein and other grain quality related traits might vary and, consequently, allow the development of waxy wheat with acceptable gluten properties. Among the waxy lines, significant variation was observed for all measured quality traits with the exception of flour protein concentration. No waxy entries statistically equaled the highest ranking nonwaxy entry for grain volume weight, falling number, flour yield, or mixograph mix time. No waxy lines numerically exceeded or equaled the mean of the nonwaxy controls for falling number, flour yield, or mixograph mix time. For grain and flour protein related variables, however, many waxy lines were identified well within the range of acceptability, relative to the nonwaxy controls used in this study. Approximately 50% of the waxy lines did not differ from the highest ranking nonwaxy cultivar for grain and flour protein concentrations. Forty-three (86%) of the tested waxy lines were not significantly different from the nonwaxy line with the highest mixograph mixing tolerance, 22/50 (44%) of the waxy wheat lines did not differ from the highest ranking nonwaxy line in gluten index scores, and 17/50 (34%) did not differ from the highest ranking nonwaxy line in extracted wet gluten. All waxy experimental lines produced gluten via Glutomatic washing. The quality of the gluten, as measured both by mixograph and gluten index, varied widely among the waxy lines tested. These observations suggest that weak gluten is not a natural consequence of the waxy trait, and waxy cultivars with acceptable gluten properties can be developed. C1 [Graybosch, R. A.] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. [Ohm, Jae-Bom; Dykes, Linda] USDA ARS, Red River Valley Agr Res Ctr, Cereal Crops Res Unit, Hard Spring & Durum Wheat Qual Lab, Fargo, ND 58102 USA. RP Graybosch, RA (reprint author), Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. EM bob.graybosch@ars.usda.gov NR 27 TC 0 Z9 0 U1 6 U2 6 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD NOV-DEC PY 2016 VL 93 IS 6 BP 599 EP 604 DI 10.1094/CCHEM-02-16-0021-R PG 6 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA EC4JZ UT WOS:000388096200011 ER PT J AU Ohm, JB Lee, CW Cho, K AF Ohm, Jae-Bom Lee, Chiwon W. Cho, Kyongshin TI Germinated Wheat: Phytochemical Composition and Mixing Characteristics SO CEREAL CHEMISTRY LA English DT Article ID PERFORMANCE LIQUID-CHROMATOGRAPHY; SIZE DISTRIBUTION; ACRYLAMIDE; PROTEINS; GRAIN; ENVIRONMENT; METABOLISM; PRODUCTS; GENOTYPE; ACID AB Germinated grain has been gaining popularity owing to its beneficial effect on human health. In this research, whole wheat samples obtained after three days and five days of germination were analyzed for biochemical components and mixing characteristics. Three days of germination caused severe initial degradation of SDS-insoluble glutenin macropolymers, which was accompanied by deterioration of mixing characteristics. Proteolysis during germination resulted in a significant increase of free amino acid concentration. For example, free asparagine concentration increased significantly after five days of germination. gamma-Aminobutyric acid concentration, which was undetectable in nongerminated grain, also increased substantially during germination. Phenolic concentration and antioxidant activity level were elevated exponentially during germination, showing a high linear correlation between them (r = 0.999, P < 0.05). These findings indicate that germinated wheat flour may be a valuable ingredient for functional foods because of its increased levels of certain phytochemical components that are beneficial to human health. C1 [Ohm, Jae-Bom] USDA ARS, Cereal Crops Res Unit, Hard Spring & Durum Wheat Qual Lab, Fargo, ND 58108 USA. [Lee, Chiwon W.] North Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Cho, Kyongshin] Washington State Univ, Coll Pharm, Expt & Syst Pharmacol, Spokane, WA 99210 USA. RP Ohm, JB (reprint author), USDA ARS, Cereal Crops Res Unit, Hard Spring & Durum Wheat Qual Lab, Fargo, ND 58108 USA. EM jae.ohm@ars.usda.gov NR 33 TC 0 Z9 0 U1 4 U2 4 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD NOV-DEC PY 2016 VL 93 IS 6 BP 612 EP 617 DI 10.1094/CCHEM-01-16-0006-R PG 6 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA EC4JZ UT WOS:000388096200013 ER PT J AU Peiris, KHS Dong, YH Bockus, WW Dowell, FE AF Peiris, Kamaranga H. S. Dong, Yanhong Bockus, William W. Dowell, Floyd E. TI Moisture Effects on the Prediction Performance of a Single-Kernel Near-Infrared Deoxynivalenol Calibration SO CEREAL CHEMISTRY LA English DT Article ID FUSARIUM HEAD BLIGHT; WHEAT KERNELS; NIR SPECTROSCOPY; ORGANIC-CARBON; SOIL-MOISTURE; WINTER-WHEAT; GRAMINEARUM; RESISTANCE; SPECTRA; WATER AB Fusarium head blight (FHB) is a serious disease in wheat that affects grain quality owing to the accumulation of mycotoxins such as deoxynivalenol (DON) in grains. Near-infrared (NIR) spectroscopy has been used to develop techniques to estimate DON levels in single wheat kernels to facilitate rapid, nondestructive screening of FHB resistance in wheat breeding lines. The effect of moisture content (MC) variation on the accuracy of single-kernel DON prediction by NIR spectroscopy was investigated. Sample MC considerably affected accuracy of the current NIR DON calibration by underestimating or overestimating DON at higher or lower moisture levels, respectively. DON in single kernels was most accurately estimated at 13-14% MC. Major NIR absorptions related to Fusarium damage were found around 1,198-1,200, 1,418-1,430, 1,698, and 1,896-1,914 nm. Major moisture related absorptions were observed around 1,162, 1,337, 1,405-1,408, 1,892-1,924, and 2,202 nm. Fusarium damage and moisture related absorptions overlapped in the 1,380-1,460 and 1,870-1,970 nm regions. These results show that absorption regions associated with water are often close to absorption regions associated with Fusarium damage. Thus, care must be taken to develop DON calibrations that are independent of grain MC. C1 [Peiris, Kamaranga H. S.] Kansas State Univ, Dept Biol & Agr Engn, Manhattan, KS 66506 USA. [Dong, Yanhong] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Bockus, William W.] Kansas State Univ, Dept Plant Pathol, Throckmorton Hall, Manhattan, KS 66506 USA. [Dowell, Floyd E.] USDA ARS, CGAHR, Stored Prod Insect & Engn Res Unit, Manhattan, KS 66502 USA. RP Dowell, FE (reprint author), USDA ARS, CGAHR, Stored Prod Insect & Engn Res Unit, Manhattan, KS 66502 USA. EM floyd.dowell@ars.usda.gov NR 35 TC 0 Z9 0 U1 4 U2 4 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD NOV-DEC PY 2016 VL 93 IS 6 BP 631 EP 637 DI 10.1094/CCHEM-04-16-0120-R PG 7 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA EC4JZ UT WOS:000388096200015 ER PT J AU Cho, K Lee, CW Ohm, JB AF Cho, Kyongshin Lee, Chiwon W. Ohm, Jae-Bom TI In Vitro Study on Effect of Germinated Wheat on Human Breast Cancer Cells SO CEREAL CHEMISTRY LA English DT Article ID APOPTOSIS; INHIBITION; GRAINS AB This research investigated the possible anticancer effects of germinated wheat flour (GWF) on growth and apoptosis of human breast cancer cells. In a series of in vitro experiments, estrogen-receptor-positive (MCF-7) and -negative (MDA-MB-231) cells were cultured and treated with GWF that was obtained after a three-day germination. The GWF significantly reduced the growth of both of MCF-7 and MDA-MB-231 cells. In analysis with a flow cytometer, the GWF significantly up-regulated apoptosis of both cell lines. These results indicate that GWF induced apoptosis and inhibited growth of the human breast cell lines, thereby demonstrating its potential inhibition of breast cancer cell proliferation. C1 [Cho, Kyongshin] Washington State Univ, Coll Pharm, Expt & Syst Pharmacol, Spokane, WA 99210 USA. [Lee, Chiwon W.] North Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Ohm, Jae-Bom] USDA ARS, Cereal Crops Res Unit, Hard Spring & Durum Wheat Qual Lab, Fargo, ND 58108 USA. RP Ohm, JB (reprint author), USDA ARS, Cereal Crops Res Unit, Hard Spring & Durum Wheat Qual Lab, Fargo, ND 58108 USA. EM jae.ohm@ars.usda.gov NR 10 TC 0 Z9 0 U1 2 U2 2 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD NOV-DEC PY 2016 VL 93 IS 6 BP 647 EP 649 DI 10.1094/CCHEM-04-16-0102-N PG 3 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA EC4JZ UT WOS:000388096200017 ER PT J AU Morey, AC Venette, RC Santacruz, ECN Mosca, LA Hutchison, WD AF Morey, Amy C. Venette, Robert C. Santacruz, Erica C. Nystrom Mosca, Laurel A. Hutchison, W. D. TI Host-mediated shift in the cold tolerance of an invasive insect SO ECOLOGY AND EVOLUTION LA English DT Article DE Epiphyas postvittana; fitness; partial freeze tolerance; polyphagy; risk assessment; supercooling point ID BROWN APPLE MOTH; EPIPHYAS-POSTVITTANA WALKER; DROSOPHILA-MELANOGASTER; TERRESTRIAL ARTHROPODS; CLIMATIC VARIABILITY; HERBIVOROUS INSECTS; THERMAL TOLERANCE; LOW-TEMPERATURES; PLANT QUALITY; TORTRICIDAE AB While many insects cannot survive the formation of ice within their bodies, a few species can. On the evolutionary continuum from freeze-intolerant (i.e., freeze-avoidant) to freeze-tolerant insects, intermediates likely exist that can withstand some ice formation, but not enough to be considered fully freeze tolerant. Theory suggests that freeze tolerance should be favored over freeze avoidance among individuals that have low relative fitness before exposure to cold. For phytophagous insects, numerous studies have shown that host (or nutrition) can affect fitness and cold-tolerance strategy, respectively, but no research has investigated whether changes in fitness caused by different hosts of polyphagous species could lead to systematic changes in cold-tolerance strategy. We tested this relationship with the invasive, polyphagous moth, Epiphyas postvittana (Walker). Host affected components of fitness, such as larval survivorship rates, pupal mass, and immature developmental times. Host species also caused a dramatic change in survival of late-instar larvae after the onset of freezing-from less than 8% to nearly 80%. The degree of survival after the onset of freezing was inversely correlated with components of fitness in the absence of cold exposure. Our research is the first empirical evidence of an evolutionary mechanism that may drive changes in cold-tolerance strategies. Additionally, characterizing the effects of host plants on insect cold tolerance will enhance forecasts of invasive species dynamics, especially under climate change. C1 [Morey, Amy C.; Santacruz, Erica C. Nystrom; Mosca, Laurel A.; Hutchison, W. D.] Univ Minnesota, Dept Entomol, St Paul, MN 55108 USA. [Venette, Robert C.] US Forest Serv, USDA, North Cent Res Stn, St Paul, MN USA. RP Morey, AC (reprint author), Univ Minnesota, Dept Entomol, St Paul, MN 55108 USA. EM morey041@umn.edu FU National Science Foundation-Integrative Graduate Education and Research Traineeship on Introduced Species and Genotypes program at the University of Minnesota [DGE-0653827]; University of Minnesota Marion Brooks Wallace and Graduate School Fellowship; University of Minnesota's Undergraduate Research Opportunity Program; US Department of Agriculture-Forest Service FX This work was supported by the National Science Foundation-Integrative Graduate Education and Research Traineeship on Introduced Species and Genotypes program at the University of Minnesota [DGE-0653827; to ACM.]; the University of Minnesota Marion Brooks Wallace and Graduate School Fellowship [to ACM.]; the University of Minnesota's Undergraduate Research Opportunity Program [to LAM.]; and the US Department of Agriculture-Forest Service [to RCV and ECN]. NR 67 TC 0 Z9 0 U1 11 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD NOV PY 2016 VL 6 IS 22 BP 8267 EP 8275 DI 10.1002/ece3.2564 PG 9 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA EB8TR UT WOS:000387664500030 PM 27878094 ER PT J AU Yuan, YQ Yu, S Banuelos, GS He, YF AF Yuan, Yongqiang Yu, Shen Banuelos, G. S. He, Yunfeng TI Accumulation of Cr, Cd, Pb, Cu, and Zn by plants in tanning sludge storage sites: opportunities for contamination bioindication and phytoremediation SO ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH LA English DT Article DE Tanning sludge storage site; Cr contamination; Phytoaccumulation; Bioindicator; Tolerant plants ID AMARANTHUS-MANGOSTANUS L.; TANNERY SLUDGE; HEAVY-METALS; PHYTOEXTRACTION CAPACITY; PHRAGMITES-AUSTRALIS; CONSTRUCTED WETLAND; CHROMIUM TOXICITY; AQUATIC PLANTS; SOILS; CADMIUM AB Tanning sludge enriched with high concentrations of Cr and other metals has adverse effects on the environment. Plants growing in the metalliferous soils may have the ability to cope with high metal concentrations. This study focuses on potentials of using native plants for bioindication and/or phytoremediation of Cr-contaminated sites. In the study, we characterized plants and soils from six tanning sludge storage sites. Soil in these sites exhibited toxic levels of Cr (averaged 16,492 mg kg(-1)) and other metals (e.g., 48.3 mg Cu kg(-1), 2370 mg Zn kg(-1), 44.9 mg Pb kg(-1), and 0.59 mg Cd kg(-1)). Different metal tolerance and accumulation patterns were observed among the sampled plant species. Phragmites australis, Zephyranthes candida, Cynodon dactylon, and Alternanthera philoxeroides accumulated moderate-high concentrations of Cr and other metals, which could make them good bioindicators of heavy metal pollution. High Cr and other metal concentrations (e.g., Cd and Pb) were found in Chenopodium rubrum (372 mg Cr kg(-1)), Aster subulatus (310 mg Cr kg(-1)), and Brassica chinensis (300 mg Cr kg(-1)), being considered as metal accumulators. In addition, Nerium indicum and Z. candida were able to tolerate high concentrations of Cr and other metals, and they may be used as preferable pioneer species to grow or use for restoration in Cr-contaminated sites. This study can be useful for establishing guidelines to select the most suitable plant species to revegetate and remediate metals in tanning sludge-contaminated fields. C1 [Yuan, Yongqiang; Yu, Shen] Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, 1799 Jimei Rd, Xiamen 301021, Peoples R China. [Banuelos, G. S.] ARS, Water Management Res Unit, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. [He, Yunfeng] Zhejiang Univ, Coll Environm & Resource Sci, 866 Yuhangtang Rd, Hangzhou 310058, Zhejiang, Peoples R China. RP Yuan, YQ (reprint author), Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, 1799 Jimei Rd, Xiamen 301021, Peoples R China. EM huayang1202@sohu.com RI Yu, Shen/C-2750-2009; CAS, KLUEH/G-8978-2016 OI Yu, Shen/0000-0002-7543-6082; FU Institute of Urban Environment, CAS; Department of Human Resources and Social Security of Fujian Province [110000A049] FX The authors are sincerely thankful to anonymous reviewers for their helpful comments on the manuscript and Irvin Arroyo in USDA ARS for providing Cr policy and concentration information in the USA and Europe. We also thank the Institute of Urban Environment, CAS, and Department of Human Resources and Social Security of Fujian Province for providing financial assistance (110000A049). NR 63 TC 1 Z9 1 U1 31 U2 31 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0944-1344 EI 1614-7499 J9 ENVIRON SCI POLLUT R JI Environ. Sci. Pollut. Res. PD NOV PY 2016 VL 23 IS 22 BP 22477 EP 22487 DI 10.1007/s11356-016-7469-4 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA EB7WW UT WOS:000387602800020 PM 27552994 ER PT J AU Yuan, YQ Yu, S Banuelos, GS He, YF AF Yuan, Yongqiang Yu, Shen Banuelos, G. S. He, Yunfeng TI Accumulation of Cr, Cd, Pb, Cu, and Zn by plants in tanning sludge storage sites: opportunities for contamination bioindication and phytoremediation (vol 23, pg 22487, 2016) SO ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH LA English DT Correction C1 [Yuan, Yongqiang; Yu, Shen] Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, 1799 Jimei Rd, Xiamen 301021, Peoples R China. [Banuelos, G. S.] ARS, USDA, Water Management Res Unit, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. [He, Yunfeng] Zhejiang Univ, Coll Environm & Resource Sci, 866 Yuhangtang Rd, Hangzhou 310058, Zhejiang, Peoples R China. RP Yu, S (reprint author), Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, 1799 Jimei Rd, Xiamen 301021, Peoples R China. EM syu@iue.ac.cn RI Yu, Shen/C-2750-2009 OI Yu, Shen/0000-0002-7543-6082 NR 1 TC 0 Z9 0 U1 4 U2 4 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0944-1344 EI 1614-7499 J9 ENVIRON SCI POLLUT R JI Environ. Sci. Pollut. Res. PD NOV PY 2016 VL 23 IS 22 BP 22488 EP 22488 DI 10.1007/s11356-016-7741-7 PG 1 WC Environmental Sciences SC Environmental Sciences & Ecology GA EB7WW UT WOS:000387602800021 PM 27696159 ER PT J AU Delgado, JA Weyers, S Dell, C Harmel, D Kleinman, P Sistani, K Leytem, A Huggins, D Strickland, T Kitchen, N Meisinger, J Del Grosso, S Johnson, J Balkcom, K Finley, J Fukagawa, N Powell, M Van Pelt, S AF Delgado, Jorge A. Weyers, Sharon Dell, Curt Harmel, Daren Kleinman, Peter Sistani, Karamat Leytem, April Huggins, David Strickland, Tim Kitchen, Newell Meisinger, John Del Grosso, Steve Johnson, Jane Balkcom, Kip Finley, John Fukagawa, Naomi Powell, Mark Van Pelt, Scott TI USDA Agricultural Research Service creates Nutrient Uptake and Outcome Network (NUOnet) SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Editorial Material C1 [Delgado, Jorge A.; Del Grosso, Steve] USDA ARS Agr Res Serv, Soil Management & Sugar Beer Res Unit, Ft Collins, CO 80522 USA. [Weyers, Sharon; Johnson, Jane] USDA ARS, Soil Management Res Unit, Morris, MN USA. [Dell, Curt; Kleinman, Peter] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA USA. [Harmel, Daren] USDA ARS, Ctr Agr Resources Res, Ft Collins, CO 80522 USA. [Sistani, Karamat] USDA ARS, Food Anim Environm Syst Res Unit, Bowling Green, KY USA. [Leytem, April] USDA ARS, Northwest Irrigat & Soils Res Unit, Kimberly, ID USA. [Huggins, David] USDA ARS, Northwest Sustainable Agroecosyst Res Unit, Pullman, WA USA. [Strickland, Tim] USDA ARS, Southeast Watershed Res Unit, Tifton, GA 31793 USA. [Kitchen, Newell] USDA ARS, Cropping Syst & Water Qual Res Unit, Columbia, MO USA. [Meisinger, John] USDA ARS, Crop Syst & Global Change Lab, Beltsville, MD USA. [Balkcom, Kip] USDA ARS, Soil Dynam Res Unit, Auburn, AL USA. [Fukagawa, Naomi] USDA ARS, Beltsville Human Nutr Res Ctr, Beltsville, MD USA. [Powell, Mark] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Van Pelt, Scott] Cropping Syst Res Lab, Wind Erosion & Water Conservat Res Unit, Lubbock, TX USA. RP Delgado, JA (reprint author), USDA ARS Agr Res Serv, Soil Management & Sugar Beer Res Unit, Ft Collins, CO 80522 USA. EM Jorge.Delgado@ars.usda.gov NR 1 TC 0 Z9 0 U1 4 U2 4 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD NOV-DEC PY 2016 VL 71 IS 6 BP 147A EP 148A DI 10.2489/jswc.71.6.147A PG 2 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA EB9RY UT WOS:000387736000004 ER PT J AU Toledo, D Sanderson, M Goslee, S Herrick, J Fults, G AF Toledo, D. Sanderson, M. Goslee, S. Herrick, J. Fults, G. TI An integrated grazingland assessment approach for range and pasturelands SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE grazingland; integrated grazingland assessment; pasture; pasture; condition score; rangeland; rangeland health ID CONDITION SCORE SYSTEM; INDICATORS; INVENTORY AB In the United States, rangelands and pasturelands have traditionally been assessed using different methods and indicators. The most commonly used rangeland health assess ment protocol on US rangelands is the Interpreting Indicators of Rangeland Health (HRH) assessment, while pasturelands are usually assessed using the Pasture Condition Score (PCS) system. The IIRH method provides a way of detecting changes in ecological attributes relative to a site's ecological potential. PCS informs managers about the utility of an area for livestock production or factors that could be keeping the area from operating at its full or optimized productive potential. There is a need for an improved grazingland assessment tool that merges the relevant elements of bOth the rangeland and pastureland assessment methods while taking into account the differing ecosystem attributes and management objectives of the grazinglands where these methods are usually applied. We use IIRH and PCS data collected at two locations in the Northern Great Plains of the United States to describe our approach and demonstrate its usefulness. We present an improved grazingland assessment protocol that is applicable to range and pasturelands that highlights the complementarity of the IIRH and PCS approaches. This improved assessment and monitoring protocol allows evaluators to assess site conditions and to make interpretations regarding management based on site-specific attributes. Standardized grazingland assessment and monitoring protocols based on ecological and land management principles will ultimately improve national level assessments and will provide a valuable and efficient tool for assessing, managing, and monitoring grazinglands. C1 [Toledo, D.; Sanderson, M.] USDA ARS, Northern Great Plains Res Lab, POB 459, Mandan, ND 58554 USA. [Goslee, S.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA USA. [Herrick, J.] USDA ARS, Res Unit Jornada, Las Cruces, NM USA. [Fults, G.] NRCS, USDA, West Natl Technol Support Ctr, Portland, OR USA. RP Toledo, D (reprint author), USDA ARS, Northern Great Plains Res Lab, POB 459, Mandan, ND 58554 USA. FU USDA Natural Resources Conservation Service; Agricultural Research Service FX This work was supported by funds from a Conservation Effects Assessment Project jointly funded by the USDA Natural Resources Conservation Service and the Agricultural Research Service. NR 22 TC 0 Z9 0 U1 3 U2 3 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD NOV-DEC PY 2016 VL 71 IS 6 BP 450 EP 459 DI 10.2489/jswc.71.6.450 PG 10 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA EB9RY UT WOS:000387736000006 ER PT J AU Johnson, DE Larson, LL Wilson, KD Clark, PE Williams, J Louhaichi, M AF Johnson, D. E. Larson, L. L. Wilson, K. D. Clark, P. E. Williams, J. Louhaichi, M. TI Cattle use of perennial streams and associated riparian areas on a northeastern Oregon landscape SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE cattle use pattern; GPS tracking collars; grazing management; land use; spatial analysis; site preference ID HOME-RANGE; LIVESTOCK; IMPACTS AB Stream and riparian health is a major concern for state and federal land management agencies that are charged with oversight of extensive land holdings in the mountain west of the United States. Several federal agencies in the 19805 and 1990s determined that livestock grazing had adversely impacted a majority of federal lands in this region. In response, management was.changed on grazing allotments to focus on stream system health. Recent advanced global positioning system (GPS) logging capabilities, both accuracy and frequency/duration of logging, coupled with rapidly developing geographic information system (GIS) analytical capabilities allowed evaluation of livestock use of streams and riparian zones on mountain rangelands. This study was undertaken to clarify spatiotemporal characteristics of cattle use of perennial streams and associated riparian areas under current US Forest Service (USFS) management and to suggest managerial strategies with the potential to maintain or improve riverine environments. We initiated a five year study in 2008 to evaluate the use by cattle of 30 m (98.4 ft) and 60 m (196.9,ft) buffers on permanent streams on three extensive study sites in northeastern Oregon.The three study sites cover 43,972 ha (108,700 ac) within a broader region 120 km (74.5 mi) north-south by 50 km (31 mi) east-west. Ten randomly selected cows from herds grazing each site were fitted with GPS collars that recorded position, date, and time at approximately five minute intervals throughout the grazing season. Nearly 3,750,000 cow positions were collected on the allotments during the study. The relative occupancy of cattle in buffers along both sides of perennial streams were determined on an annual and monthly basis by site. Each position was tagged with the date and time of occurrence. Relative use within 30 m of the stream varied substantially from site to site (0.74% to 2.54%), month to month (0.00% to 5.21%), and year to year (0.86% to 2.13%). In some months, GPS data indicated that watering was nearly exclusive from streams. In other months, stream use was low or nil, and watering was from water developments, small springs, seeps, puddles, or other source. Cattle preferred to access streams at specific locations where streambank slope, the lack of physical obstructions, and solid footing facilitated water access. C1 [Johnson, D. E.; Williams, J.] Oregon State Univ, Dept Anim & Rangeland Sci, Corvallis, OR 97331 USA. [Wilson, K. D.] NRCS, USDA, Pendleton, OR USA. [Clark, P. E.] USDA ARS, Boise, ID 83712 USA. [Louhaichi, M.] ICARDA, Amman, Jordan. RP Johnson, DE (reprint author), Oregon State Univ, Dept Anim & Rangeland Sci, Corvallis, OR 97331 USA. FU USDA National Institute of Food and Agriculture [2010-85101-20512, 5362-13610-009-06R]; Oregon Beef Council; Oregon State University/Oregon Agricultural Experiment Station; USDA Agricultural Research Service; University of Idaho; International Center for Agricultural Research in the Dry Areas (ICARDA) FX The authors gratefully acknowledge the following organizations and individuals for funding, cooperation, and technical and logistic support: (1) USDA National Institute of Food and Agriculture ("Developing an Adaptive Management System and its Components to Address Effects of Gray Wolf Reintroduction on Ecosystem Services" Agency Number: 2010-85101-20512 and "Development Of Planning and Monitoring Elements of an Adaptive Management System for Wolf-Livestock Relations" NIFA Project No. 5362-13610-009-06R), (2) Oregon Beef Council, (3) Oregon State University/Oregon Agricultural Experiment Station, (4) USDA Agricultural Research Service, (5) University of Idaho, (6) International Center for Agricultural Research in the Dry Areas (ICARDA), and (7) farm and ranch families on the research sites. Animals were handled in accordance with Oregon State University animal handling protocols under permit ACUP # 4555. NR 36 TC 0 Z9 0 U1 12 U2 12 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD NOV-DEC PY 2016 VL 71 IS 6 BP 484 EP 493 DI 10.2489/jswc.71.6.484 PG 10 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA EB9RY UT WOS:000387736000010 ER PT J AU Johnson, DE Clark, PE Larson, LL Wilson, KD Louhaichi, M Freeburg, T Williams, J AF Johnson, D. E. Clark, P. E. Larson, L. L. Wilson, K. D. Louhaichi, M. Freeburg, T. Williams, J. TI Cattle use of off-stream water developments across a northeastern Oregon landscape SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE cattle riparian use; livestock water placement; off-stream water developments ID RIPARIAN ZONE; LIVESTOCK; QUALITY; ECOSYSTEMS; IMPACTS; SALT AB Water developments have been considered a fundamental tool for dispersing livestock and reducing livestock impact on riparian and aquatic habitats associated with perennial streams. Quantifying the efficacy of water developments has been difficult because of the need to continuously monitor water sources for the timing and intensity of use. The advent of global positioning system (GPS) tracking technology has largely overcome this hurdle and presented new research opportunities. We conducted a five-year study to quantify and evaluate the relative use of existing water developments by cattle on three extensive study areas in northeastern Oregon, United States. Ten randomly selected beef cows from herds grazing each study area were fitted with GPS collars recording animal positions at five-minute intervals throughout the grazing season. Cattle occupancy in 60 m (196.9 ft) buffers around water developments was determined monthly and annually. Use of water developments was contrasted with that of riparian zones. Cattle use of water developments varied substantially from site-to-site, month-to-month, and year-to-year. In some months, cattle watered exclusively from off-stream developments, and in others, cattle watered nearly exclusively from streams. Substantial differences in the relative use of individual water developments were also seen by season and year. Some developments received no use at all during the five-year study while others were visited frequently. Our results suggest off-stream water development is a useful managerial strategy, but careful placement is required to improve the likelihood that cattle will find and use these water sources and thus decrease their dependence and use of permanent streams and associated riparian areas. C1 [Johnson, D. E.; Larson, L. L.; Williams, J.] Oregon State Univ, Dept Anim & Rangeland Sci, Corvallis, OR 97331 USA. [Clark, P. E.] USDA ARS, Boise, ID 83712 USA. [Wilson, K. D.] Nat Resources Conservat Serv, USDA, Pendleton, OR USA. [Louhaichi, M.] Int Ctr Agr Res Dry Areas, Amman, Jordan. [Freeburg, T.] Univ Idaho Extens, Moscow, ID USA. RP Johnson, DE (reprint author), Oregon State Univ, Dept Anim & Rangeland Sci, Corvallis, OR 97331 USA. FU USDA National Institute of Food and Agriculture [2010-85101-20512, 5362-13610-009-06R]; Oregon Beef Council; Oregon State University/Oregon Agricultural Experiment Station; USDA/Agricultural Research Service; University of Idaho; International Center for Agricultural Research in the Dry Areas (ICARDA); farm and ranch families FX The authors gratefully acknowledge the following organizations and individuals for funding, cooperation, and technical and logistic support: (1) USDA National Institute of Food and Agriculture ("Developing an Adaptive Management System and its Components to Address Effects of Gray Wolf Reintroduction on Ecosystem Services" Agency Number: 2010-85101-20512 and "Development Of Planning and Monitoring Elements of an Adaptive Management 426 System for Wolf-Livestock Relations" NIFA Project No. 5362-13610-009-06R), (2) Oregon Beef Council, (3) Oregon State University/Oregon Agricultural Experiment Station, (4) USDA/Agricultural Research Service, (5) University of Idaho, (6) International Center for Agricultural Research in the Dry Areas (ICARDA), and (7) farm and ranch families on the research sites. Animals were handled in accordance with Oregon State University animal handling protocols under permit ACUP # 4555. NR 42 TC 0 Z9 0 U1 8 U2 8 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD NOV-DEC PY 2016 VL 71 IS 6 BP 494 EP 502 DI 10.2489/jswc.71.6.494 PG 9 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA EB9RY UT WOS:000387736000011 ER PT J AU Williams, JD Robertson, DS AF Williams, J. D. Robertson, D. S. TI Soil water in small drainages farmed with no-tillage and inversion tillage in northeastern Oregon SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE dryland systems; no-tillage; Pacific Northwest; precipitation use efficiency ID SEMIARID PACIFIC-NORTHWEST; WINTER-WHEAT; CROPPING SYSTEMS; MANAGEMENT INFLUENCE; EROSION; CONSERVATION; FALLOW; PRECIPITATION; PRODUCTIVITY; TEMPERATURE AB Crop productivity in the semiarid inland Pacific Northwest, United States, is dependent on the capture and storage of precipitation as soil water. To maximize soil water in this region, the conventional crop strategy is a two-year cropping system in which winter wheat (Triticum aestivum L.) is grown after a 14 month fallow period. This system is popular in the intermediate precipitation zone (300 to 450 mm [12 to 18 in]) in northeastern Oregon because of its history of producing reliable yields under highly variable precipitation. Adopting no-tillage and increasing the types of crops and frequency with which they are grown has the potential to improve soil quality and crop productivity. Water is the primary limiting factor in this region. Evaluating how increased cropping intensity and no-tillage affect the soil water regime is key to understanding the performance of these practices. Crops were grown in two small, upland drainages using inversion tillage or no-tillage. The inversion tillage drainage was in a two-year winter wheat-fallow rotation (W-f-F), from 2001 through 2004. From 2005 through 2008, it was divided in two parts with W-f-F in one half and annual cropping in the second half for adaptive management where the following were grown: recropped wheat (W-r), volunteer wheat (W-v), and wheat recropped after volunteer wheat W-w. The no-tillage drainage was divided into four roughly equal areas and farmed in a four-year rotation, which was completed twice in eight years (2001 to 2008). The four phases of the rotation were chemical fallow-winter wheat-spring pea (Cicer arietinum L. or Pisum sativum L.)winter wheat (CF-W-cf-SP-W-sp). Mean crop yield was not significantly different between inversion tillage (2.98 +/- 0.30 Mg ha(-1) [1.33 +/- 0.14 tn ac(-1)]) and no-tillage (3.14 +/- 0.29 Mg ha(-1) [1.40 +/- 0.13 tn ac(-1)]). Crop yield in each rotation phase was significantly different (p <= 0.05) in the following ranked groups: W-cf similar to W-f similar to W-w similar to W-sp similar to W-r > W-v > SP. Contrary to this expectation, precipitation use efficiency (PUE) was significantly greater in the inversion tillage (0.019 +/- 0.002 Mg ha(-1) mm(-1) [7.0 +/- 0.6 bu ac(-1) in(-1)]) than in the no-tillage (0.015 +/- 0.001 Mg ha(-1) mm(-1) [5.5 +/- 0.5 bu ac(-1) in(-1)]) drainage. However, PUE among phases within each system differed significantly in the following ranked largest to smallest order: W-f similar to W-cf, W-w similar to W-sp similar to W-r similar to W-v, and SP. There was significantly more available water at the 30 cm (1 ft) depth in Wcf before fall planting than in the W-f. Early attempts at adopting no-tillage in northeastern Oregon were unsuccessful, generally attributed to poor soil water conditions, particularly near the surface. Based on crop yields reported here, it appears that more recent no-tillage technology and management of crop residue has alleviated this concern. A wider variety of alternative spring crops is needed to more thoroughly evaluate and compare PUE in intensified cropping systems to the traditional two-year winter wheat/fallow system practiced in the intermediate precipitation zone in northeastern Oregon. C1 [Williams, J. D.; Robertson, D. S.] USDA ARS, Columbia Plateau Conservat Res Ctr, Pendleton, OR 97801 USA. RP Williams, JD (reprint author), USDA ARS, Columbia Plateau Conservat Res Ctr, Pendleton, OR 97801 USA. FU USDA ARS national programs Agricultural System Competitiveness and Sustainability [216] FX The authors thank cooperating landowner Clinton Reeder and faculty from the Columbia Basin Agriculture Research Center-Oregon State University (Dan Ball [emeritus professor], Richard Smiley [emeritus professor], and Don Wysocki [associate professor]) for advice concerning weed, disease, and crop management. Field work, instrumentation, laboratory analysis, and data collection were conducted by Daryl Haasch (hydrologic technician, Columbia Plateau Conservation Research Center - USDA Agricultural Research Service [ARS]-Columbia Plateau Conservation Research Center [CPCRC], retired), Bob Correa (agricultural research technician - USDA ARS-CPCRC, retired), Tami Johlke (biological science technician CPCRC USDA ARS), Chris Roager (chemist - USDA ARS-CPCRC, retired), Scott Oviatt (snow survey supervisor, USDA Natural Resources Conservation Service [MRCS]), and numerous high school and college students. We appreciate the construcdve comments made by two anonymous reviewers and our CPCRC colleagues Tami Johlke and Catherine Reardon (research microbiologist) and Stewart bluest (soil scientist). This research was conducted under USDA ARS national programs Agricultural System Competitiveness and Sustainability (NP#216) and Climate Change, Soils, and Emissions (NP#212). NR 36 TC 0 Z9 0 U1 7 U2 7 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD NOV-DEC PY 2016 VL 71 IS 6 BP 503 EP 511 DI 10.2489/jswc.71.6.503 PG 9 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA EB9RY UT WOS:000387736000012 ER PT J AU Henderson, RM Lovato, L Miller, ME Fielding, RA Church, TS Newman, AB Buford, TW Pahor, M McDermott, MM Stafford, RS Lee, DSH Kritchevsky, SB AF Henderson, Rebecca M. Lovato, Laura Miller, Michael E. Fielding, Roger A. Church, Tim S. Newman, Anne B. Buford, Thomas W. Pahor, Marco McDermott, Mary M. Stafford, Randall S. Lee, David S. H. Kritchevsky, Stephen B. CA LIFE Study Investigators TI Effect of Statin Use on Mobility Disability and its Prevention in At-risk Older Adults: The LIFE Study SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES LA English DT Article DE Physical activity; Physical function; Functional performance; Exercise ID PERIPHERAL ARTERIAL-DISEASE; SKELETAL-MUSCLE FUNCTION; PHYSICAL-ACTIVITY; STYLE INTERVENTIONS; EXERCISE; INDEPENDENCE; PERFORMANCE; THERAPY; DECLINE; ELDERS AB HMG-CoA reductase inhibitors (statins) are among the most commonly prescribed classes of medications. Although their cardiovascular benefits and myalgia risks are well documented, their effects on older adults initiating an exercise training program are less understood. 1,635 sedentary men and women aged 70-89 years with Short Physical Performance Battery (SPPB) score of 9 or below and were able to walk 400 m were randomized to a structured, moderate-intensity physical activity (PA) program consisting of both center-based (twice/wk) and home-based (3-4 times/wk) aerobic, resistance, and flexibility training or to a health education (HE) program combined with upper extremity stretching. Overall, the PA intervention was associated with lower risk of major mobility disability (hazard ratio [HR] = 0.82; 95% confidence interval [CI] = 0.69-0.98). The effect was similar (p value for interaction = .62) in both statin users (PA n = 415, HE n = 412; HR = 0.86, 95% CI = 0.67-1.1) and nonusers (PA n = 402, HE n = 404; HR = 0.78, 95% CI = 0.61-1.01). Attendance was similar for statin users (65%) and nonusers (63%). SPPB at 12 months was slightly greater for PA (8.35 +/- 0.10) than for HE (7.94 +/- 0.10) in statin users but not in nonusers (PA 8.25 +/- 0.10, HE 8.16 +/- 0.10), though the interaction effect was not statistically significant. Self-reported PA levels were not different between statin users and nonusers. Although statins have been associated with adverse effects on muscle, data from the LIFE Study show that statin users and nonusers both benefit from PA interventions. Older adults who require statin medications to manage chronic medical conditions and are sedentary will be able to benefit from interventions to increase PA. C1 [Henderson, Rebecca M.; Kritchevsky, Stephen B.] Wake Forest Sch Med, Sticht Ctr Aging, Sect Gerontol & Geriatr Med, Dept Internal Med, Winston Salem, NC USA. [Lovato, Laura; Miller, Michael E.] Wake Forest Univ Hlth Sci, Dept Biostat Sci, Winston Salem, NC USA. [Fielding, Roger A.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. [Church, Tim S.] Louisiana State Univ Syst, Pennington Biomed Res Ctr, Baton Rouge, LA USA. [Newman, Anne B.] Univ Pittsburgh, Dept Epidemiol, Pittsburgh, PA 15260 USA. [Buford, Thomas W.; Pahor, Marco] Northwestern Univ, Feinberg Sch Med, Dept Med, Chicago, IL USA. [McDermott, Mary M.] Univ Florida, Coll Med, Dept Aging & Geriatr Res, Gainesville, FL USA. [Stafford, Randall S.] Stanford Sch Med, Stanford Prevent Res Ctr, Palo Alto, CA USA. [Lee, David S. H.] Oregon State Univ, Coll Pharm, Corvallis, OR 97331 USA. [Lee, David S. H.] Oregon Hlth & Sci Univ, Portland, OR 97201 USA. RP Henderson, RM (reprint author), Wake Forest Univ, Sch Med, Dept Internal Med, Med Ctr Blvd, Winston Salem, NC 27157 USA. EM rmyer@wakehealth.edu RI Buford, Thomas /B-1111-2011 OI Buford, Thomas /0000-0002-9541-4358 FU National Institutes of Health, National Institute on Aging [UO1AG22376]; National Heart, Lung, and Blood Institute [3U01AG022376-05A2S]; Intramural Research Program; Claude D. Pepper Older Americans Independence Centers [1P30AG031679, 1P30AG028740, P30AG024827, 1P30AG21332, P30AG021342] FX This work was supported by the National Institutes of Health, National Institute on Aging (UO1AG22376), the National Heart, Lung, and Blood Institute (3U01AG022376-05A2S), the Intramural Research Program, and the Claude D. Pepper Older Americans Independence Centers (1P30AG031679, 1P30AG028740, P30AG024827, 1P30AG21332, and P30AG021342). NR 22 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1079-5006 EI 1758-535X J9 J GERONTOL A-BIOL JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci. PD NOV PY 2016 VL 71 IS 11 BP 1519 EP 1524 DI 10.1093/gerona/glw057 PG 6 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA EC3AG UT WOS:000387995800019 PM 26988662 ER PT J AU Broadhurst, CL Schmidt, WF Kim, MS Nguyen, JK Qin, JW Chao, KL Bauchan, GL Shelton, DR AF Broadhurst, C. Leigh Schmidt, Walter F. Kim, Moon S. Nguyen, Julie. K. Qin, Jianwei Chao, Kuanglin Bauchan, Gary L. Shelton, Daniel. R. TI Continuous Gradient Temperature Raman Spectroscopy of Oleic and Linoleic Acids from-100 to 50 degrees C SO LIPIDS LA English DT Article DE Gradient temperature Raman spectroscopy; Oleic acid; Linoleic acid; Polyunsaturated fatty acids; Raman spectroscopy ID FTIR SPECTROSCOPY; PHASE-TRANSITIONS; ADIPOSE-TISSUE; SPECTRA; POLYMORPHISM; INSPECTION; DISORDER; SYSTEM; LIPIDS; GAMMA AB We analyzed the unsaturated fatty acids oleic (OA, 18:1n-9) and linoleic (LA, 18:2n-3), and a 3:1 LA:OA mixture from -100 to 50 degrees C with continuous gradient temperature Raman spectroscopy (GTRS). The 20 Mb three-dimensional data arrays with 0.2 degrees C increments and first/second derivatives allowed rapid, complete assignment of solid, liquid, and transition state vibrational modes. For OA, large spectral and line width changes occurred in the solid state. to a transition near -4 degrees C, and the melt (13 degrees C) over a range of only 1 degrees C. For LA, major intensity reductions from 200 to 1750 cm(-1) and some peak shifts marked one solid state phase transition at -50 degrees C. A second solid state transition (-33 degrees C) had minor spectral changes. Large spectral and line width changes occurred at the melt transition (-7 degrees C) over a narrow temperature range. For both molecules, melting initiates at the diene structure, then progresses towards the ends. In the 3:1 LA: OA mixture, some less intense and lower frequencies present in the individual lipids are weaker or absent. For example, modes assignable to C8 rocking, C9H-C10H wagging, C10H-C11H wagging, and CH3 rocking are present in OA but absent in LA:OA. Our data quantify the concept of lipid premelting and identify the flexible structures within OA and LA, which have characteristic vibrational modes beginning at cryogenic temperatures. C1 [Broadhurst, C. Leigh; Schmidt, Walter F.; Kim, Moon S.; Nguyen, Julie. K.; Qin, Jianwei; Chao, Kuanglin; Shelton, Daniel. R.] USDA ARS, Environm Microbiol & Food Safety Lab, Bldg 161 Rm 116,10300 Baltimore Ave, Beltsville, MD 20705 USA. [Broadhurst, C. Leigh] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Bauchan, Gary L.] ARS, Electron & Confocal Microscopy Unit, Inst Plant Sci, USDA, Beltsville, MD 20705 USA. RP Broadhurst, CL (reprint author), USDA ARS, Environm Microbiol & Food Safety Lab, Bldg 161 Rm 116,10300 Baltimore Ave, Beltsville, MD 20705 USA.; Broadhurst, CL (reprint author), Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. EM leigh.broadhurst@ars.usda.gov NR 35 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0024-4201 EI 1558-9307 J9 LIPIDS JI Lipids PD NOV PY 2016 VL 51 IS 11 BP 1289 EP 1302 DI 10.1007/s11745-016-4194-1 PG 14 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA EC5OD UT WOS:000388184500007 PM 27663253 ER PT J AU Trupia, S Winkler-Moser, JK Guney, AC Beckstead, R Chen, CYO AF Trupia, S. Winkler-Moser, J. K. Guney, A. C. Beckstead, R. Chen, C-Y. O. TI Nutritional quality of eggs from hens fed distillers dried grains with solubles SO POULTRY SCIENCE LA English DT Article DE distiller's dried grains with solubles; laying hens; egg production; egg color; nutritional quality ID FATTY-ACID; LAYING HENS; VITAMIN-E; CAROTENOIDS; FEED; HPLC; YOLK; EXTRACTS; DIETS AB A feeding trial was conducted with laying hens where either 10% or 20% regular-fat distiller's dried grains with solubles (R-DDGS) or low-fat DDGS (L-DDGS) were incorporated into the feed. Production parameters and the effect of DDGS on egg nutritional quality, focusing on yolk lipids, were evaluated. Neither R-DDGS nor L-DDGS at up to 20% of laying hen feeds had a statistically significant impact on hen weight gain, egg production, feed intake, feed efficiency, egg mass, or egg weight. Specific gravity was slightly lower for eggs from hens fed 10% R-DDGS or 20% L-DDGS. Eggs from layers fed DDGS had enhanced levels of tocopherols, tocotrienols, and xanthophylls in the yolk, as well as also increased yolk yellow and red color. Eggs from L-DDGS diet had higher tocopherol content, but eggs from R-DDGS diets had higher xanthophylls. Fatty acid composition in eggs was slightly altered by DDGS, but the ratio of saturated to unsaturated fatty acids was very similar. Feeding DDGS to layer hens had no effect on lecithin or cholesterol content of the eggs. Thus, inclusion of DDGS in the diet of laying hens resulted in increases of several beneficial lipophilic nutrients in egg yolks with no apparent detrimental effects. C1 [Trupia, S.] Southern Illinois Univ Edwardsville, Natl Corn To Ethanol Res Ctr, 400 Univ Pk Dr, Edwardsville, IL 62026 USA. [Winkler-Moser, J. K.] ARS, USDA, NCAUR, Funct Foods Res Unit, Peoria, IL USA. [Guney, A. C.; Beckstead, R.] Univ Georgia, Dept Poultry Sci, Athens, GA 30602 USA. [Chen, C-Y. O.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Medford, MA 02155 USA. RP Trupia, S (reprint author), Southern Illinois Univ Edwardsville, Natl Corn To Ethanol Res Ctr, 400 Univ Pk Dr, Edwardsville, IL 62026 USA. EM strupia@ethanolresearch.com FU Illinois Corn Marketing Board; Iowa Corn Marketing Board; Indiana Corn Marketing Board FX Support of this research was generously provided by the Illinois, Iowa and Indiana Corn Marketing Boards. The authors would like to acknowledge Deb Palmquist for statistical analyses and discussions, and Kathy Rennick, Nathalie Gonzalez Jimenez, and Patrick Schneider for their excellent technical assistance. NR 34 TC 0 Z9 0 U1 2 U2 2 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD NOV PY 2016 VL 95 IS 11 BP 2592 EP 2601 DI 10.3382/ps/pew142 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA EC3KC UT WOS:000388024000013 PM 27118865 ER PT J AU Guo, M Ma, YT Zhao, ZC Ma, M Wu, XJ Mankin, RW AF Guo, Min Ma, Yuting Zhao, Zichen Ma, Miao Wu, Xiaojun Mankin, Richard W. TI A New EEMD-Based Scheme for Detection of Insect Damaged Wheat Kernels Using Impact Acoustics SO ACTA ACUSTICA UNITED WITH ACUSTICA LA English DT Article ID PISTACHIO NUTS; FAULT-DIAGNOSIS; EMISSIONS; SYSTEM; SHELL; FEASIBILITY; HAZELNUTS; DESIGN; POTATO; GRAIN AB Internally feeding insects inside wheat kernels cause significant, but unseen economic damage to stored grain. In this paper, a new scheme based on ensemble empirical mode decomposition (EEMD) using impact acoustics is proposed for detection of insect-damaged wheat kernels, based on its capability to process non-stationary signals and its suppression of mode mixing. The intrinsic mode function (IMF) kurtosis, IMF form factors, IMF third-order Renyi entropies, and the mean of the degree of stationarity were extracted as discriminant features used as the inputs to a support vector machine (SVM) for non-linear classification. In these experiments, 98.7% of undamaged wheat kernels and 93.3% of insect-damaged ones were correctly detected, which indicated the effectiveness of the proposed method for categorizing undamaged wheat kernels from insect-damaged wheat kernels (IDK). C1 [Guo, Min; Ma, Yuting; Ma, Miao; Wu, Xiaojun] Shaanxi Normal Univ, Sch Comp Sci, Xian 710062, Peoples R China. [Zhao, Zichen; Mankin, Richard W.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Guo, M (reprint author), Shaanxi Normal Univ, Sch Comp Sci, Xian 710062, Peoples R China. EM guomin@snnu.edu.cn FU National Natural Science Foundation of China [11172342, 11372167]; Science Research and Development Program of Shaanxi Province of China [2016NY-176]; Fundamental Research Funds for the Central Universities [GK201405007]; Interdisciplinary Incubation Project of Learning Science of Shaanxi Normal University FX This work was supported by the National Natural Science Foundation of China (No. 11172342, 11372167), the Science Research and Development Program of Shaanxi Province of China (No. 2016NY-176), the Fundamental Research Funds for the Central Universities (No. GK201405007) and Interdisciplinary Incubation Project of Learning Science of Shaanxi Normal University. NR 25 TC 1 Z9 1 U1 3 U2 3 PU S HIRZEL VERLAG PI STUTTGART PA POSTFACH 10 10 61, D-70 009 STUTTGART, GERMANY SN 1610-1928 EI 1861-9959 J9 ACTA ACUST UNITED AC JI Acta Acust. United Acust. PD NOV-DEC PY 2016 VL 102 IS 6 BP 1108 EP 1117 DI 10.3813/AAA.919022 PG 10 WC Acoustics SC Acoustics GA EB8JB UT WOS:000387636200012 ER PT J AU Latshaw, SP Vigil, MF Haley, SD AF Latshaw, Susan P. Vigil, Merle F. Haley, Scott D. TI Genotypic Differences for Nitrogen Use Efficiency and Grain Protein Deviation in Hard Winter Wheat SO AGRONOMY JOURNAL LA English DT Article ID TRITICUM-AESTIVUM L.; DRY-MATTER; NEGATIVE RELATIONSHIP; BREAD WHEAT; REGISTRATION; YIELD; REMOBILIZATION; ACCUMULATION; IMPROVEMENT; SENESCENCE AB Breeding superior bread wheat (Triticum aestivum L.) genotypes requires sufficient genetic variation to obtain high grain yield and adequate grain protein concentration. This study was conducted to determine variation for nitrogen use efficiency (NUE) and grain protein deviation among 20 hard winter wheat genotypes in one season and for two recently released cultivars (Snowmass and Byrd) in a second season, under five N application rates (0, 28, 56, 84, 112 kg ha(-1)). Among these genotypes, the proportionate contributions of component traits to total variance for NUE ranged widely: N uptake efficiency (57-89 kg kg(-1)) and N utilization efficiency (11-43 kg kg(-1)). Across all genotypes, N utilization efficiency contributed the most to variance for NUE under moderate to high N supply while N uptake efficiency contributed more under N limiting conditions. Increased NUE promotes high grain yields, but may result in decreased grain protein concentration through the commonly observed negative correlation of these traits. Analysis of residuals from regression of grain protein concentration on grain yield, or "grain protein deviation", identified one genotype (Brawl CL Plus) that had 6.7 g kg(-1) higher grain protein concentration than the average for all 20 genotypes. These results for a representative sample of a breeding population suggest that sufficient variation is available to improve NUE and grain protein deviation through breeding. C1 [Latshaw, Susan P.; Haley, Scott D.] Colorado State Univ, Soil & Crop Sci Dept, 1170 Campus Delivery, Ft Collins, CO 80523 USA. [Latshaw, Susan P.] Bayer CropSci LP, 925 Cty Rd 378, Beaver Crossing, NE 68313 USA. [Vigil, Merle F.] ARS, USDA, NPA Cent Great Plains Res Stn, 40335 Cty Rd GG, Akron, CO 80720 USA. RP Haley, SD (reprint author), Colorado State Univ, Soil & Crop Sci Dept, 1170 Campus Delivery, Ft Collins, CO 80523 USA. EM scott.haley@colostate.edu FU USDA-ARS Central Plains Resources Management Research Unit, Akron, CO, under National Program 212, Soils and Global Climate change CRIS Project [3010-12210-002-00D] FX The authors would like to thank Dr. Mark Brick (CSU) and Dr. Ken Barbarick (CSU) for their careful reviews of this manuscript. Assistance with statistical analysis was provided by Dr. Philip Chapman (CSU). We are also grateful to David J. Poss and Linda Hardesty (USDA-ARS) for field management and for performing the C/N analyses and to Emily Hudson-Arns, Scott Seifert, and John Stromberger (CSU) for planting, managing, and harvesting the trials. Tawney Campbell, James Hamilton, Donald (Collin) Dutro, and Erin Krause assisted with data collection and field support. Funding was provided by the USDA-ARS Central Plains Resources Management Research Unit, Akron, CO, under National Program 212, Soils and Global Climate change CRIS Project number 3010-12210-002-00D. NR 59 TC 0 Z9 0 U1 18 U2 18 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD NOV-DEC PY 2016 VL 108 IS 6 BP 2201 EP 2213 DI 10.2134/agronj2016.02.0070 PG 13 WC Agronomy SC Agriculture GA EB7GV UT WOS:000387555700004 ER PT J AU Harbach, C Chawla, S Bowen, CR Hill, CB Nafziger, ED Hartman, GL AF Harbach, C. Chawla, S. Bowen, C. R. Hill, C. B. Nafziger, E. D. Hartman, G. L. TI Association of Green Stem Disorder with Agronomic Traits in Soybean SO AGRONOMY JOURNAL LA English DT Article ID SENESCENCE; CULTIVARS; NUCLEAR AB Green stem disorder (GSD) of soybean [Glycine max (L.) Merr.] is the occurrence of non-senescent, fleshy green stems of plants with normal, fully mature pods and seeds. The main focus of this study was to determine the relationship between GSD incidence and agronomic traits and to determine if GSD incidence was associated with soybean cultivars, years, location of trials, and rainfall. Data on GSD incidence based on a percentage of plants in plots showing symptoms were collected for soybean cultivars in 86 trials from 2009 to 2012 at seven locations in Illinois. The incidence of GSD ranged from 0% (three trials) to 88% with a mean incidence of 12% averaged over the 83 trials. The GSD incidence was correlated with data on yield, plant height and lodging, and seed moisture, protein, and oil content for all trials. The incidence of GSD was positively correlated (P < 0.05) with yield (12 cases), plant height (24) and lodging (29), and seed moisture (35), protein (19), and oil content (2), and negatively correlated (P < 0.05) with yield (eight cases), plant height (2) and lodging (3), and seed moisture (1), protein (3), and oil content (24). Correlations among agronomic traits, GSD, and in-season precipitation, indicated that yield and precipitation (May, June, and July) were negatively correlated (P < 0.05) with GSD. The incidence of GSD is a result of the genetics of soybean cultivars and how they interact with the environment. C1 [Harbach, C.; Chawla, S.; Bowen, C. R.; Hill, C. B.; Nafziger, E. D.; Hartman, G. L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Bowen, C. R.; Hartman, G. L.] ARS, USDA, Urbana, IL 61801 USA. RP Hartman, GL (reprint author), Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. EM ghartman@illinois.edu NR 16 TC 0 Z9 0 U1 5 U2 5 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD NOV-DEC PY 2016 VL 108 IS 6 BP 2263 EP 2268 DI 10.2134/agronj2016.03.0155 PG 6 WC Agronomy SC Agriculture GA EB7GV UT WOS:000387555700010 ER PT J AU Nielsen, DC Vigil, MF Hansen, NC AF Nielsen, David C. Vigil, Merle F. Hansen, Neil C. TI Evaluating Potential Dryland Cropping Systems Adapted to Climate Change in the Central Great Plains SO AGRONOMY JOURNAL LA English DT Article ID AIR CO2 ENRICHMENT; WATER-CONTENT; WINTER-WHEAT; FALLOW; YIELD; SOIL; IMPACTS; PRECIPITATION; RESPONSES; CROPS AB Climate in the semiarid central Great Plains is expected to become warmer and drier in coming decades, with potentially greater variability in precipitation and temperature. Cropping systems that include forages and allow flexibility for determining if a crop should be planted and which crop to plant (based on available soil water at planting) may provide the opportunity to maintain economic viability in a changing climate environment. The objective of this study was to compare cropping system productivity and profitability of flexible rotations that incorporate forages against grain-based cropping systems that are set rotational sequences. Yield and net returns for five set rotations and three flexible rotations were compared at Akron, CO, over 5 yr. Winter wheat (Triticum aestivum L.) yields were reduced by 57% when the fallow period prior to wheat production was replaced with crop production. Average net income was greatest for the continuously cropped all-forage set 3-yr rotation followed by the flexible 3-yr rotations that included wheat and forage phases. The lowest net returns were seen for the set grain-based rotations and the flexible wheat-grain crop rotation. Incorporating forage production as a phase in dryland wheat rotational systems can add profitability and sustainability to the production system in the face of climate variability. C1 [Nielsen, David C.; Vigil, Merle F.] USDA ARS, Cent Great Plains Res Stn, 40335 Cty Rd GG, Akron, CO 80720 USA. [Hansen, Neil C.] Brigham Young Univ, 5108 LSB, Provo, UT 84602 USA. RP Nielsen, DC (reprint author), USDA ARS, Cent Great Plains Res Stn, 40335 Cty Rd GG, Akron, CO 80720 USA. EM david.nielsen@ars.usda.gov NR 42 TC 1 Z9 1 U1 4 U2 4 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD NOV-DEC PY 2016 VL 108 IS 6 BP 2391 EP 2405 DI 10.2134/agronj2016.07.0406 PG 15 WC Agronomy SC Agriculture GA EB7GV UT WOS:000387555700023 ER PT J AU Cho, AH Chase, CA Koenig, RL Treadwell, DD Gaskins, J Morris, JB Morales-Payan, JP AF Cho, Alyssa H. Chase, Carlene A. Koenig, Rosalie L. Treadwell, Danielle D. Gaskins, Jeremy Morris, J. Bradley Morales-Payan, J. Pablo TI Phenotypic Characterization of 16 Accessions of Sunn Hemp in Florida SO AGRONOMY JOURNAL LA English DT Article ID LEGUME COVER CROP; CROTALARIA-JUNCEA; SYSTEMS; BEES AB Adoption of sunn hemp (Crotalaria juncea L.) as a cover crop has been limited primarily due to the availability of seed sources, leading to high seed costs and unreliable supplies. Seed production in Florida with the commercially available sunn hemp cultivar Tropic Sun has been largely unsuccessful. An experiment was designed to evaluate 16 accessions of sunn hemp for potential commercial seed production in Florida at three planting dates (May, June, July). Vegetative and reproductive characteristics were evaluated to provide baseline information for future development of a cultivar of sunn hemp that could produce seed in Florida with desirable cover crop attributes. The results indicate that the sunn hemp accessions could be separated into two groups. Regardless of planting date, accessions in Group 1 (PI 234771, PI 248491, PI 295851, PI 337080, PI 468956, PI 561720, PI 652939) displayed desirable cover crop characteristics, such as high biomass production and leaf area. Accessions in Group 1 had a short-day flowering response and produced few to no pods and seeds. Group 2 accessions (PI 207657, PI 250485, PI 250486, PI 250487, PI 314239, PI 322377, PI 346297, PI 391567, PI 426626) were less sensitive to photoperiod, were generally smaller plants because of earlier transition to reproductive growth, and produced more seedpods and seeds. Seed production was generally better when seeds were sown at the earliest date. It appears that in Florida, sunn hemp accessions that are capable of flowering in summer rather than fall have greater potential for seed production. C1 [Cho, Alyssa H.; Chase, Carlene A.; Treadwell, Danielle D.] Univ Florida, Hort Sci Dept, POB 110690, Gainesville, FL 32611 USA. [Koenig, Rosalie L.] Univ Florida, IFAS Global, Gainesville, FL 32611 USA. [Gaskins, Jeremy] Univ Louisville, Dept Bioinformat & Biostat, Louisville, KY 40202 USA. [Morris, J. Bradley] USDA ARS, Griffin, GA 30223 USA. [Morales-Payan, J. Pablo] Univ Puerto Rico Mayaguez, Dept Agroenvironm Sci, Call Box 9000, Mayaguez, PR 00681 USA. [Cho, Alyssa H.] Univ Hawaii Manoa, Dept Trop Plant & Soil Sci, Honolulu, HI 96822 USA. RP Chase, CA (reprint author), Univ Florida, Hort Sci Dept, POB 110690, Gainesville, FL 32611 USA. EM cachase@ufl.edu FU Southern Region Sustainable Agriculture Research and Education Grant [LS08-205] FX This study was partially funded by the Southern Region Sustainable Agriculture Research and Education Grant LS08-205. NR 20 TC 0 Z9 0 U1 8 U2 8 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD NOV-DEC PY 2016 VL 108 IS 6 BP 2417 EP 2424 DI 10.2134/agronj2015.0531 PG 8 WC Agronomy SC Agriculture GA EB7GV UT WOS:000387555700025 ER PT J AU Pariz, CM Costa, C Crusciol, CAC Meirelles, PRL Castilhos, AM Andreotti, M Costa, NR Martello, JM Souza, DM Sarto, JRW Franzluebbers, AJ AF Pariz, Cristiano M. Costa, Ciniro Crusciol, Carlos A. C. Meirelles, Paulo R. L. Castilhos, Andre M. Andreotti, Marcelo Costa, Nidia R. Martello, Jorge M. Souza, Daniel M. Sarto, Jaqueline R. W. Franzluebbers, Alan J. TI Production and Soil Responses to Intercropping of Forage Grasses with Corn and Soybean Silage SO AGRONOMY JOURNAL LA English DT Article ID CROP-LIVESTOCK SYSTEM; NO-TILLAGE SYSTEM; GRAIN-YIELD; NITROGEN-FERTILIZATION; CHEMICAL-COMPOSITION; PALISADE GRASS; MAIZE; TIME; DECOMPOSITION; CERRADO AB Agricultural management systems are needed to simultaneously enhance production, and improve soil quality. We investigated the effects of intercropped grass on production of corn (Zea mays L.) harvested for silage at 0.20 and 0.45 m height in the summer, as well as on production of subsequent forage, silage soybean [Glycine max (L.) Merr.], and soil responses on a Typic Haplorthox in Botucatu, SP, Brazil. Palisade grass [Urochloa brizantha (Hochst. ex A. Rich.) R. Webster 'Marandu'] was the introduced companion crop with corn (Years 1 and 2), while signal grass [Urochloa decumbens (Stapf) R. Webster 'Basilisk'] was the residual weedy species in comparison. Guinea grass [Urochloa maxima (Jacq.) R. Webster 'Aruana'] was the introduced companion crop with soybean (Year 3), with only a residual effect of crop systems from the previous 2 yr. When cut at 0.45 m compared with 0.20 m height, corn intercropped with palisade grass had greater leaf nutrient concentrations, agronomic characteristics, forage mass of pasture for grazing by lambs (Ovis aries), greater surface mulch produced, and greater quantity of N, P, and K returned to soil. Greater soil organic matter, P, K, and Mg concentration, and base saturation in the surface soil depth and lower soil penetration resistance at all depths occurred at 0.45 m than at 0.20 m corn silage cutting height intercropped with palisade grass. Analyzing the system as a whole, harvesting corn silage crop with palisade grass intercrop at 0.45 m height was the most viable option in this integrated crop-livestock system (ICLS). C1 [Pariz, Cristiano M.; Costa, Ciniro; Meirelles, Paulo R. L.; Castilhos, Andre M.; Souza, Daniel M.; Sarto, Jaqueline R. W.] Sao Paulo State Univ UNESP, Coll Vet Med & Anim Sci, Dept Anim Nutr & Breeding, Botucatu, SP, Brazil. [Crusciol, Carlos A. C.; Costa, Nidia R.; Martello, Jorge M.] UNESP, Coll Agr Sci, Dept Crop Sci, Botucatu, SP, Brazil. [Andreotti, Marcelo] UNESP, Coll Engn, Dept Soil Sci, Ilha Solteira, SP, Brazil. [Franzluebbers, Alan J.] North Carolina State Univ, USDA ARS, 3218 Williams Hall,Campus Box 7619, Raleigh, NC 27695 USA. RP Pariz, CM (reprint author), Sao Paulo State Univ UNESP, Coll Vet Med & Anim Sci, Dept Anim Nutr & Breeding, Botucatu, SP, Brazil.; Crusciol, CAC (reprint author), UNESP, Coll Agr Sci, Dept Crop Sci, Botucatu, SP, Brazil.; Franzluebbers, AJ (reprint author), North Carolina State Univ, USDA ARS, 3218 Williams Hall,Campus Box 7619, Raleigh, NC 27695 USA. EM cmpzoo@gmail.com; crusciol@fca.unesp.br; alan.franzluebbers@ars.usda.gov RI Magalhaes Pariz, Cristiano/C-3154-2012; Costa, Ciniro/C-6487-2012 FU Sao Paulo Research Foundation (FAPESP); Foundation for the development of UNESP (FUNDUNESP) [2011/12155-3, 2010/12992-0, 0538/011/14-PROPe/CDC]; Coordination of Improvement of Higher Education Personnel (CAPES); National Council for Scientific and Technological Development (CNPq) FX The authors would like to thank the Sao Paulo Research Foundation (FAPESP) and the Foundation for the development of UNESP (FUNDUNESP) for the financial support (grant no. 2011/12155-3 and no. 2010/12992-0) and (grant no. 0538/011/14-PROPe/CDC), as well as the Coordination of Improvement of Higher Education Personnel (CAPES) for the grant to the first author and the National Council for Scientific and Technological Development (CNPq) for an award for excellence in research to the second, third, and sixth authors. NR 63 TC 1 Z9 1 U1 14 U2 14 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD NOV-DEC PY 2016 VL 108 IS 6 BP 2541 EP 2553 DI 10.2134/agronj2016.02.0082 PG 13 WC Agronomy SC Agriculture GA EB7GV UT WOS:000387555700037 ER PT J AU Bista, P Machado, S Ghimire, R Del Grosso, SJ Reyes-Fox, M AF Bista, Prakriti Machado, Stephen Ghimire, Rajan Del Grosso, Stephen J. Reyes-Fox, Melissa TI Simulating Soil Organic Carbon in a Wheat-Fallow System Using the Daycent Model SO AGRONOMY JOURNAL LA English DT Article ID NITROUS-OXIDE EMISSIONS; LONG-TERM EXPERIMENTS; GREENHOUSE-GAS MITIGATION; PACIFIC-NORTHWEST; NO-TILL; CROPPING SYSTEMS; ECOSYSTEM MODEL; SEQUESTRATION RATES; RESIDUE MANAGEMENT; BIOFUEL PRODUCTION AB Crop management practices that contribute to soil organic carbon (SOC) sequestration can improve productivity and long-term sustainability. A simulation study was conducted using the DAYCENT model over an 80-yr period. The objectives of the study were to assess model performance and forecast SOC changes in conventional tillage and no-tillage management in a dryland winter wheat (Triticum aestivum L.)-summer fallow (WW-SF) system. The treatments studied included fall burning of crop residue (FB0), no burning of crop residue with 0 (NB0), 45 (NB45) and 90 (NB90) kg N ha(-1), pea vines (PV), and cattle manure (MN) addition. The model was accurate with R-2 values of 0.93 (p < 0.01), 0.95 (p < 0.01), and 0.99 (p < 0.01) for the mean of observed and modeled grain yield, residue yield, and SOC, respectively. The strong positive correlation (r = 0.71-0.91) in different treatments between observed and modeled SOC indicated that model closely simulated the observed values. DAYCENT projected that conventionally tilled WW-SF systems, except MN, lost 866 to 2192 g C m(-2) SOC from 1931 to 2080. The MN gained 496 g C m(-2) SOC in the same period. The conversion to no-tillage from 2011 onward, however, minimized SOC loss by 17 to 47% under different WW-SF systems. No-tillage conversion in MN resulted in SOC gain by more than 300%. This study suggested that adoption of a no-tillage system and the addition of organic amendments can improve the long-term sustainability of dryland WW-SF systems. C1 [Bista, Prakriti; Machado, Stephen] Oregon State Univ, Columbia Basin Agr Res Ctr, 48037 Tubbs Ranch Rd,POB 370, Pendleton, OR 97801 USA. [Ghimire, Rajan] New Mexico State Univ, Agr Sci Ctr Clovis, 2346 State Rd 288, Clovis, NM 88101 USA. [Del Grosso, Stephen J.; Reyes-Fox, Melissa] USDA ARS, Soil Plant Nutrient Res Unit, Ft Collins, CO 80526 USA. RP Bista, P (reprint author), Oregon State Univ, Columbia Basin Agr Res Ctr, 48037 Tubbs Ranch Rd,POB 370, Pendleton, OR 97801 USA. EM prakriti.bista@oregonstate.edu FU National Institute of Food and Agriculture, U.S. Department of Agriculture [2011-68002-30191]; Columbia Basin Agricultural Research Center FX This paper is based on work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award number 2011-68002-30191 and additional support from the Columbia Basin Agricultural Research Center. We are grateful to Bill Parton, Senior Research Scientist at Natural Resource Ecology lab, Colorado State University for his suggestions regarding model runs. We thank Karl Rhinhart, Pendleton Station Farm Manager for detail information on long-term experiments in Columbia Basin Agricultural Research Center at Pendleton, OR. NR 83 TC 0 Z9 0 U1 10 U2 10 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD NOV-DEC PY 2016 VL 108 IS 6 BP 2554 EP 2565 DI 10.2134/agronj2016.04.0202 PG 12 WC Agronomy SC Agriculture GA EB7GV UT WOS:000387555700038 ER PT J AU Short, FT Kosten, S Morgan, PA Malone, S Moore, GE AF Short, Frederick T. Kosten, Sarian Morgan, Pamela A. Malone, Sparkle Moore, Gregg E. TI Impacts of climate change on submerged and emergent wetland plants SO AQUATIC BOTANY LA English DT Article DE Global climate change; Seagrass; Freshwater plants; Mangroves; Marsh; Submergent plants ID SEA-LEVEL RISE; EELGRASS ZOSTERA-MARINA; FRESH-WATER MACROPHYTES; UV-B RADIATION; SHALLOW EUTROPHIC LAKES; ELEVATED CARBON-DIOXIDE; SALT-MARSH; POSIDONIA-OCEANICA; THALASSIA-TESTUDINUM; MANGROVE FORESTS AB Submerged and emergent wetland plant communities are evaluated for their response to global climate change (GCC), focusing on seagrasses, submerged freshwater plants, tidal marsh plants, freshwater marsh plants and mangroves. Similarities and differences are assessed in plant community responses to temperature increase, CO2 increase, greater UV-B exposure, sea level rise and other expected environmental alterations associated with GCC. Responses to most climate change variables are more similar within submerged plant communities, marine or freshwater, than between submerged vs. emergent plant communities. The submerged plants are most affected by temperature increases and indirect impacts on water clarity. Emergent plant communities (marshes and mangroves) respond most directly to climate change related hydrological alterations. Wetland plant communities overall appear to be adversely impacted by all climate change variables, with the exception of increased CO2 in the atmosphere and the oceans, which in most cases increases photosynthesis. Effects of GCC on all these communities have already been seen with many others predicted, including: shifts in species composition, shifts in range and distribution, and declines in plant species richness. Other effects are associated with specific community types, e.g., salt marsh habitat lost to mangrove incursion, and decreases in submerged macrophyte coverage in lakes and estuaries, exacerbated by eutrophication. Sea level rise poses threats to all aquatic plant community types in the vicinity of the oceans, and changes in weather patterns and salinity will affect many. Overall, losses are likely in all these wetland plant communities yet their species can adapt to GCC to some degree if well managed and protected. (C) 2016 Elsevier B.V. All rights reserved. C1 [Short, Frederick T.] Univ New Hampshire, Dept Nat Resources & Environm, Jackson Estuarine Lab, 85 Adams Point Rd, Durham, NH 03824 USA. [Kosten, Sarian] Radboud Univ Nijmegen, Inst Water & Wetland Res, Aquat Ecol & Environm Biol Dept, Heyendaalseweg 135, NL-6525 AJ Nijmegen, Netherlands. [Morgan, Pamela A.] Univ New England, Dept Environm Studies, Biddeford, ME 04005 USA. [Malone, Sparkle] USFS Rocky Mt Res Stn, 240 W Prospect Rd, Ft Collins, CO 80526 USA. [Moore, Gregg E.] Univ New Hampshire, Dept Biol Sci, Jackson Estuarine Lab, 85 Adams Point Rd, Durham, NH 03824 USA. RP Short, FT (reprint author), Univ New Hampshire, Dept Nat Resources & Environm, Jackson Estuarine Lab, 85 Adams Point Rd, Durham, NH 03824 USA. EM fred.short@unh.edu RI Kosten, Sarian/D-7550-2011 NR 197 TC 1 Z9 1 U1 238 U2 238 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3770 EI 1879-1522 J9 AQUAT BOT JI Aquat. Bot. PD NOV PY 2016 VL 135 SI SI BP 3 EP 17 DI 10.1016/j.aquabot.2016.06.006 PG 15 WC Plant Sciences; Marine & Freshwater Biology SC Plant Sciences; Marine & Freshwater Biology GA EB8HI UT WOS:000387631700002 ER PT J AU Cook, JA Greiman, SE Agosta, SJ Anderson, RP Arbogast, BS Baker, RJ Boeger, W Bradley, RD Brooks, DR Cole, R Demboski, JR Dobson, AP Dunnum, JL Eckerlin, RP Esselstyn, J Galbreath, KE Hawdon, J Hoekstra, HE Kutz, SJ Light, JE Olson, LE Patterson, BD Patton, JL Phillips, AJ Rickart, E Rogers, DS Siddall, ME Tkach, VV Hoberg, EP AF Cook, Joseph A. Greiman, Stephen E. Agosta, Salvatore J. Anderson, Robert P. Arbogast, Brian S. Baker, Robert J. Boeger, Walter Bradley, Robert D. Brooks, Daniel R. Cole, Rebecca Demboski, John R. Dobson, Andrew P. Dunnum, Jonathan L. Eckerlin, Ralph P. Esselstyn, Jacob Galbreath, Kurt E. Hawdon, John Hoekstra, Hopi E. Kutz, Susan J. Light, Jessica E. Olson, Link E. Patterson, Bruce D. Patton, James L. Phillips, Anna J. Rickart, Eric Rogers, Duke S. Siddall, Mark E. Tkach, Vasyl V. Hoberg, Eric P. TI Transformational Principles for NEON Sampling of Mammalian Parasites and Pathogens: A Response to Springer and Colleagues SO BIOSCIENCE LA English DT Editorial Material ID CLIMATE-CHANGE; ECOLOGY; DISEASE; COLLECTIONS C1 [Cook, Joseph A.; Greiman, Stephen E.; Dunnum, Jonathan L.] Univ New Mexico, Museum Southwestern Biol, Albuquerque, NM 87131 USA. [Cook, Joseph A.; Greiman, Stephen E.; Dunnum, Jonathan L.] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. [Agosta, Salvatore J.] Virginia Commonwealth Univ, Ctr Environm Studies, Richmond, VA 23284 USA. [Agosta, Salvatore J.] Virginia Commonwealth Univ, Dept Biol, Richmond, VA 23284 USA. [Anderson, Robert P.] CUNY City Coll, Dept Biol, 138th St & Convent Ave, New York, NY 10031 USA. [Arbogast, Brian S.] Univ North Carolina Wilmington, Dept Biol & Marine Biol, Wilmington, NC USA. [Baker, Robert J.; Bradley, Robert D.] Texas Tech Univ, Dept Biol, Lubbock, TX 79409 USA. [Baker, Robert J.; Bradley, Robert D.] Texas Tech Univ Museum, Lubbock, TX 79409 USA. [Boeger, Walter] Univ Fed Parana, Curitiba, Parana, Brazil. [Brooks, Daniel R.] Univ Nebraska, HW Manter Lab Parasitol, Lincoln, NE USA. [Cole, Rebecca] US Geol Survey, Natl Wildlife Hlth Ctr, Madison, WI USA. [Demboski, John R.] Denver Museum Nat & Sci, Denver, CO USA. [Dobson, Andrew P.] Princeton Univ, Dept Ecol & Evolutionary Biol, Princeton, NJ 08544 USA. [Eckerlin, Ralph P.] Northern Virginia Community Coll, Math Sci & Engn Div, Annandale, VA USA. [Esselstyn, Jacob] Louisiana State Univ, Museum Nat Sci, Baton Rouge, LA 70803 USA. [Esselstyn, Jacob] Louisiana State Univ, Dept Biol Sci, Baton Rouge, LA 70803 USA. [Galbreath, Kurt E.] Northern Michigan Univ, Dept Biol, Marquette, MI USA. [Hawdon, John] George Washington Univ, Sch Med & Hlth Sci, Washington, DC 20052 USA. [Hoekstra, Hopi E.] Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA. [Hoekstra, Hopi E.] Harvard Univ, Museum Comparat Zool, Cambridge, MA 02138 USA. [Kutz, Susan J.] Univ Calgary, Fac Vet Med, Calgary, AB T2N 1N4, Canada. [Light, Jessica E.] Texas A&M Univ, Dept Wildlife & Fisheries Sci, College Stn, TX 77843 USA. [Light, Jessica E.] Texas A&M Univ, Biodivers Res & Teaching Collect, College Stn, TX USA. [Olson, Link E.] Univ Alaska, Univ Alaska Museum, Fairbanks, AK 99701 USA. [Patterson, Bruce D.] Field Museum Nat Hist, Chicago, IL 60605 USA. [Patton, James L.] Univ Calif Berkeley, Museum Vertebrate Zool, Berkeley, CA 94720 USA. [Phillips, Anna J.; Hoberg, Eric P.] Smithsonian Inst, Natl Museum Nat Hist, Washington, DC 20560 USA. [Hoberg, Eric P.] ARS, USDA, Beltsville, MD USA. [Rickart, Eric] Nat Hist Museum, Salt Lake City, UT USA. [Rogers, Duke S.] Brigham Young Univ, Monte L Bean Life Sci Museum, Provo, UT 84602 USA. [Rogers, Duke S.] Brigham Young Univ, Dept Biol, Provo, UT 84602 USA. [Siddall, Mark E.] Amer Museum Nat Hist, New York, NY 10024 USA. [Tkach, Vasyl V.] Univ North Dakota, Dept Biol, Grand Forks, ND USA. RP Cook, JA (reprint author), Univ New Mexico, Museum Southwestern Biol, Albuquerque, NM 87131 USA.; Cook, JA (reprint author), Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. EM tucojoe@gmail.com OI Demboski, John/0000-0002-5163-4113 NR 13 TC 0 Z9 0 U1 4 U2 4 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0006-3568 EI 1525-3244 J9 BIOSCIENCE JI Bioscience PD NOV PY 2016 VL 66 IS 11 BP 917 EP 919 DI 10.1093/biosci/biw123 PG 3 WC Biology SC Life Sciences & Biomedicine - Other Topics GA EB6AU UT WOS:000387463900002 ER PT J AU Lowe, AJ Dormontt, EE Bowie, MJ Degen, B Gardner, S Thomas, D Clarke, C Rimbawanto, A Wiedenhoeft, A Yin, YF Sasaki, N AF Lowe, Andrew J. Dormontt, Eleanor E. Bowie, Matthew J. Degen, Bernd Gardner, Shelley Thomas, Darren Clarke, Caitlin Rimbawanto, Anto Wiedenhoeft, Alex Yin, Yafang Sasaki, Nophea TI Opportunities for Improved Transparency in the Timber Trade through Scientific Verification SO BIOSCIENCE LA English DT Article DE certification; illegal logging; scientific verification; timber trade; wood identification ID SPECIES IDENTIFICATION; WOOD IDENTIFICATION; DNA; ORIGIN; CITES; DISCRIMINATION; CONCESSION AB In May 2014, the Member States of the United Nations adopted Resolution 23/1 on "strengthening a targeted crime prevention and criminal justice response to combat illicit trafficking in forest products, including timber." The resolution promotes the development of tools and technologies that can be used to combat the illicit trafficking of timber. Stopping illegal logging worldwide could substantially increase revenue from the legal trade in timber and halt the associated environmental degradation, but law enforcement and timber traders themselves are hampered by the lack of available tools to verify timber legality. Here, we outline how scientific methods can be used to verify global timber supply chains. We advocate that scientific methods are capable of supporting both enforcement and compliance with respect to timber laws but that work is required to expand the applicability of these methods and provide the certification, policy, and enforcement frameworks needed for effective routine implementation. C1 [Lowe, Andrew J.; Dormontt, Eleanor E.; Bowie, Matthew J.; Sasaki, Nophea] Univ Adelaide, Sch Biol Sci, Ctr Conservat Sci & Technol, Adelaide, SA 5005, Australia. [Lowe, Andrew J.; Thomas, Darren; Clarke, Caitlin] Double Helix Tracking Technol, Singapore, Singapore. [Degen, Bernd] Thunen Inst Forest Genet, Grosshansdorf, Germany. [Gardner, Shelley] US Forest Serv, USDA, Int Programs, Washington, DC 20250 USA. [Gardner, Shelley] US Dept Justice, INTERPOL Washington, Econ Crimes Div, Washington, DC 20530 USA. [Rimbawanto, Anto] FORDA Ctr Forest Biotechnol & Tree Improvement, Yogyakarta, Indonesia. [Wiedenhoeft, Alex] US Forest Serv, USDA, Forest Prod Lab, Madison, WI USA. [Yin, Yafang] Chinese Acad Forestry, Res Inst Wood Ind, Dept Wood Anat & Utilizat, Beijing, Peoples R China. [Sasaki, Nophea] Univ Hyogo, Grad Sch Appl Informat, Kobe, Hyogo, Japan. RP Lowe, AJ (reprint author), Univ Adelaide, Sch Biol Sci, Ctr Conservat Sci & Technol, Adelaide, SA 5005, Australia.; Lowe, AJ (reprint author), Double Helix Tracking Technol, Singapore, Singapore. EM andrew.lowe@adelaide.edu.au RI Degen, Bernd/S-1730-2016; Yin, Yafang/C-1568-2017 FU ITTO grant [TFL-PD 037/13]; ACIAR grant [FST/2014/028, FST-2015-007] FX This work was funded through ITTO grant no. TFL-PD 037/13 and ACIAR grants nos. FST/2014/028 and FST-2015-007 awarded to AJL. The authors gratefully acknowledge the US Department of Justice's Joseph Poux, the UK Border Agency's Guy Clarke, and Environment Canada's Jean-Francois Dubois for their constructive comments on table 1. The authors also thank Dr. Rob Ogden, Dr. Edgard Espinoza, Dr. Martin Breed, and the three anonymous reviewers for their constructive feedback, which greatly improved the quality of the manuscript. NR 33 TC 1 Z9 1 U1 13 U2 13 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0006-3568 EI 1525-3244 J9 BIOSCIENCE JI Bioscience PD NOV PY 2016 VL 66 IS 11 BP 990 EP 998 DI 10.1093/biosci/biw129 PG 9 WC Biology SC Life Sciences & Biomedicine - Other Topics GA EB6AU UT WOS:000387463900011 ER PT J AU Pigeot, I Baranowski, T Lytle, L Ahrens, W AF Pigeot, Iris Baranowski, Tom Lytle, Leslie Ahrens, Wolfgang TI Prevention of Overweight and Obesity in Children and Adolescents Critical Appraisal of the Evidence Base SO BUNDESGESUNDHEITSBLATT-GESUNDHEITSFORSCHUNG-GESUNDHEITSSCHUTZ LA German DT Article ID SCHOOL-BASED INTERVENTION; PHYSICAL-ACTIVITY; CHILDHOOD OBESITY; SEDENTARY BEHAVIOR; DIETARY INTERVENTIONS; CONTROLLED-TRIALS; FOOD ENVIRONMENT; BODY-COMPOSITION; HEALTH OUTCOMES; DOWNWARD TRENDS AB Despite careful planning and implementation, overweight/obesity prevention interventions in children and adolescents typically show no, inconsistent or merely weak effects. Such programs usually aim at behavior changes, rarely also at environmental changes, that draw upon conventional wisdom regarding the commonly accepted determinants of childhood overweight/obesity. This paper evaluates the evidence base of the apparently overweight-/obesity-related determinants diet, physical activity and stress. The results of international intervention studies are discussed against this background. Based on the mediating-moderating variable model, we investigate the effect of theory specified mediating variables and how potential moderating variables may impact these relationships. Contrary to common beliefs, recent research has revealed inconsistent evidence regarding associations between potentially obesogenic behaviors and overweight/obesity in youth. Moreover, the evidence for strong and causal relationships between mediating variables and targeted behaviors seems to be inconsistent. In addition, inadequate attention is paid to moderating effects. The etiology of overweight/obesity in youth is likely the result of a complex interplay of multi-causal influences. Future prevention interventions would benefit from a more thorough understanding of the complex relationships that have been hypothesized and of the mechanisms of suspected behaviors for affecting overweight/obesity. Only if substantial change can be demonstrated in mediators with reasonable effort under real world circumstances, it will make sense to progress to community behavior change trials. C1 [Pigeot, Iris; Ahrens, Wolfgang] Univ Bremen, Leibniz Inst Pravent Forsch & Epidemiol BIPS, Fachbereich Math & Informat 03, Achterstr 30, D-28359 Bremen, Germany. [Baranowski, Tom] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Lytle, Leslie] Univ North Carolina Chapel Hill, Sch Publ Hlth, Dept Hlth Behav, Chapel Hill, NC USA. RP Pigeot, I (reprint author), Univ Bremen, Leibniz Inst Pravent Forsch & Epidemiol BIPS, Fachbereich Math & Informat 03, Achterstr 30, D-28359 Bremen, Germany. EM pigeot@leibniz-bips.de NR 73 TC 0 Z9 0 U1 9 U2 9 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1436-9990 EI 1437-1588 J9 BUNDESGESUNDHEITSBLA JI Bundesgesundheitsblatt-Gesund. PD NOV PY 2016 VL 59 IS 11 BP 1423 EP 1431 DI 10.1007/s00103-016-2449-x PG 9 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA EB1DQ UT WOS:000387087800007 PM 27709262 ER PT J AU Dao, TH AF Dao, Thanh H. TI Instantaneous accounting for leaf water in X-ray fluorescence spectra of corn grown in manure- and fertilizer-amended soils SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE Labile phosphorus status; In situ foliar analysis; Spectroscopic nutrient sensing; X-ray fluorescence; Foliar macronutrient sensing; Crop P status; Real time foliar water content ID PHOSPHORUS FRACTIONS; INORGANIC PHOSPHORUS; MEDICINAL-PLANTS; MAIZE; MANAGEMENT; NUTRITION; DYNAMICS AB Manure-amended soils have shown a high degree of temporal and geographic variability in labile phosphorus (P). Real time information and practical ways for determining foliar composition are needed to obtain direct evidence of plant uptake, and the supplying capacity of the soil. Field and laboratory experiments were conducted to determine leaf water content effects on spectral characteristics of corn (Zea mays) uppermost leaves when grown in mineral P fertilizers- (0-250 mg P kg(-1)) or manure-amended soils (0, 15, and 30 kg P ha(-1)). In situ X-ray fluorescence scans of fresh green leaves yielded foliar P concentrations that paralleled those obtained using oven-dried harvested leaf samples. Leaf structure and size of seedlings can cause significant variations in leaf-to-spectrometer contact, inter-element interference, and leaf water content and its attenuating effects on the yield of weak fluorescence radiation of low Z-elements (<19). Intensities of scattered radiation associated with the X-ray tube anode were significantly correlated with leaf water content (theta(w)), which was used to (i) assess crop water status and (ii) normalize fluorescence intensities of P to a common basis. The theta(w) - P reduced concentration relationship was best-described by a sigmoidal function y = 0.12 + 0.94/(1 + exp(-(theta(w) - 0.37)/-0.17)) with r(2) = 0.938 and RMSE = 0.02. Therefore, we proposed its use to obtain P concentration on a dry weight basis and unbiased estimates of crop P status. The in situ fluorescence sensing system presents a new paradigm in nutrient management to insure the sustainability of agroecosystems and development of field specific nutrient management practices. Published by Elsevier B.V. C1 [Dao, Thanh H.] USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Dao, TH (reprint author), USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM thanh.dao@ars.usda.gov NR 35 TC 0 Z9 0 U1 3 U2 3 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 EI 1872-7107 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD NOV 1 PY 2016 VL 129 BP 84 EP 90 DI 10.1016/j.compag.2016.09.012 PG 7 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA EB3WE UT WOS:000387298800010 ER PT J AU Aronson, MFJ Nilon, CH Lepczyk, CA Parker, TS Warren, PS Cilliers, SS Goddard, MA Hahs, AK Herzog, C Katti, M La Sorte, FA Williams, NSG Zipperer, W AF Aronson, Myla F. J. Nilon, Charles H. Lepczyk, Christopher A. Parker, Tommy S. Warren, Paige S. Cilliers, Sarel S. Goddard, Mark A. Hahs, Amy K. Herzog, Cecilia Katti, Madhusudan La Sorte, Frank A. Williams, Nicholas S. G. Zipperer, Wayne TI Hierarchical filters determine community assembly of urban species pools SO ECOLOGY LA English DT Article DE biodiversity; city; community assembly; filters; human-dominated landscapes; urban ecology; UrBioNet ID NEW-YORK-CITY; PLANT TRAITS; BIODIVERSITY CONSERVATION; PITTOSPORUM-UNDULATUM; SOCIOECONOMIC-STATUS; CLIMATE-CHANGE; DOMESTIC GARDENS; BIRD COMMUNITIES; TROPHIC DYNAMICS; CENTRAL VICTORIA AB The majority of humanity now lives in cities or towns, with this proportion expected to continue increasing for the foreseeable future. As novel ecosystems, urban areas offer an ideal opportunity to examine multi-scalar processes involved in community assembly as well as the role of human activities in modulating environmental drivers of biodiversity. Although ecologists have made great strides in recent decades at documenting ecological relationships in urban areas, much remains unknown, and we still need to identify the major ecological factors, aside from habitat loss, behind the persistence or extinction of species and guilds of species in cities. Given this paucity of knowledge, there is an immediate need to facilitate collaborative, interdisciplinary research on the patterns and drivers of biodiversity in cities at multiple spatial scales. In this review, we introduce a new conceptual framework for understanding the filtering processes that mold diversity of urban floras and faunas. We hypothesize that the following hierarchical series of filters influence species distributions in cities: (1) regional climatic and biogeographical factors; (2) human facilitation; (3) urban form and development history; (4) socioeconomic and cultural factors; and (5) species interactions. In addition to these filters, life history and functional traits of species are important in determining community assembly and act at multiple spatial scales. Using these filters as a conceptual framework can help frame future research needed to elucidate processes of community assembly in urban areas. Understanding how humans influence community structure and processes will aid in the management, design, and planning of our cities to best support biodiversity. C1 [Aronson, Myla F. J.] Rutgers State Univ, Dept Ecol Evolut & Nat Resources, New Brunswick, NJ 08901 USA. [Nilon, Charles H.] Univ Missouri, Sch Nat Resources, Columbia, MO 65211 USA. [Lepczyk, Christopher A.] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. [Parker, Tommy S.] Univ Memphis, Ecol Res Ctr, Memphis, TN 38152 USA. [Warren, Paige S.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [Cilliers, Sarel S.] North West Univ, Unit Environm Sci & Management, ZA-2520 Potchefstroom, South Africa. [Goddard, Mark A.] Newcastle Univ, Sch Civil Engn & Geosci, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England. [Hahs, Amy K.; Williams, Nicholas S. G.] Univ Melbourne, Sch Biosci, Australian Res Ctr Urban Ecol, Royal Bot Gardens Victoria, Melbourne, Vic 3010, Australia. [Herzog, Cecilia] Pontificia Univ Catolica Rio de Janeiro, Dept Architecture & Urbanism, BR-22451900 Rio De Janeiro, Brazil. [Katti, Madhusudan] North Carolina State Univ, Dept Forestry & Environm Resources, Chancellors Fac Excellence Program Leadership Pub, Raleigh, NC 27695 USA. [La Sorte, Frank A.] Cornell Univ, Cornell Lab Ornithol, Ithaca, NY 14850 USA. [Williams, Nicholas S. G.] Univ Melbourne, Sch Ecosyst & Forest Sci, Richmond, Vic 3121, Australia. [Zipperer, Wayne] US Forest Serv, USDA, Southern Res Stn, Gainesville, FL 32611 USA. RP Aronson, MFJ (reprint author), Rutgers State Univ, Dept Ecol Evolut & Nat Resources, New Brunswick, NJ 08901 USA. EM myla.aronson@rutgers.edu RI Goddard, Mark/D-1166-2010 OI Goddard, Mark/0000-0002-2283-6752 FU National Science Foundation (NSF RCN: DEB) [1354676/1355151] FX We appreciate the valuable comments from two anonymous reviewers that helped refine our thoughts. We would like to acknowledge financial support for this work from the National Science Foundation (NSF RCN: DEB # 1354676/1355151). NR 141 TC 0 Z9 0 U1 36 U2 36 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0012-9658 EI 1939-9170 J9 ECOLOGY JI Ecology PD NOV PY 2016 VL 97 IS 11 BP 2952 EP 2963 DI 10.1002/ecy.1535 PG 12 WC Ecology SC Environmental Sciences & Ecology GA EB2YJ UT WOS:000387228200007 PM 27870023 ER PT J AU Chapin, FS Conway, AJ Johnstone, JF Hollingsworth, TN Hollingsworth, J AF Chapin, F. Stuart, III Conway, Alexandra J. Johnstone, Jill F. Hollingsworth, Teresa N. Hollingsworth, Jamie TI Absence of net long-term successional facilitation by alder in a boreal Alaska floodplain SO ECOLOGY LA English DT Article DE Alaska; alder; boreal forest; competition; facilitation; floodplain; nitrogen fixation; primary succession; structural equation modeling ID TANANA RIVER FLOODPLAIN; INTERIOR ALASKA; GLACIER BAY; SAND DUNES; VEGETATION; ECOSYSTEMS; DYNAMICS; FOREST; TAIGA; BIOGEOCHEMISTRY AB Long-term experiments provide a way to test presumed causes of successional or environmentally driven vegetation changes. Early-successional nitrogen (N)-fixing plants are widely thought to facilitate productivity and vegetation development on N-poor sites, thus accounting for observed vegetation patterns later in succession. We tested this facilitative impact on vegetation development in a 23-yr field experiment on an Interior Alaska (USA) floodplain. On three replicate early-successional silt bars, we planted late-successional white spruce (Picea glauca) seedlings in the presence and absence of planted seedlings of an early-successional N-fixing shrub, thinleaf alder (Alnus incana). Alder initially facilitated survivorship and growth of white spruce. Within six years, however, after canopy closure, alder negatively affected spruce survivorship and growth. Our three replicate sites followed different successional trajectories. One site was eliminated by erosion and supported no vegetation development during our study. The other two sites, which differed in site moisture, diverged in vegetation composition. Structural equation modeling (SEM) suggested that, in the drier of these two sites, alder inhibited spruce growth directly (presumably by competition) and indirectly through effects mediated by competition with other woody species. However, at the wetter site, alder had both positive and negative effects on spruce growth, with negative effects predominating. Snowshoe hares (Lepus americanus) in alder thickets further reduced height growth of spruce in the wetter site. We conclude that net effects of alder on white spruce, the late-successional dominant, were primarily inhibitory and indirect, with the mechanisms depending on initial site moisture. Our results highlight the importance of long-term research showing that small differences among initial replicate sites can cause divergence in successional trajectories, consistent with individualistic distributions of species and communities along environmental gradients. This divergence was detectable only decades later. C1 [Chapin, F. Stuart, III; Hollingsworth, Jamie] Univ Alaska, Inst Arctic Biol, Fairbanks, AK 99775 USA. [Conway, Alexandra J.; Johnstone, Jill F.] Univ Saskatchewan, Dept Biol, 112 Sci Pl, Saskatoon, SK S7N 5E2, Canada. [Hollingsworth, Teresa N.] US Forest Serv, USDA, Pacific Northwest Res Stn, Fairbanks, AK 99775 USA. RP Chapin, FS (reprint author), Univ Alaska, Inst Arctic Biol, Fairbanks, AK 99775 USA. EM terry.chapin@alaska.edu OI Johnstone, Jill/0000-0001-6131-9339 FU National Science Foundation [8702629, 9211769, 9810217]; USDA Forest Service [PNW 96-5024-2-CA, PNW01-JV11261952-231, PNW06-JV-11261952-431] FX This research was supported by grants from the National Science Foundation grants (8702629, 9211769, and 9810217) and the USDA Forest Service (PNW 96-5024-2-CA, PNW01-JV11261952-231, and PNW06-JV-11261952-431) to the Bonanza Creek LTER Program. We thank Phyllis Adams, Jenna Baker, Brian Charlton, Shalane Frost, Melanie Jean, Erin Julianus, Knut Kielland, Lori Moilanen, Jonny Newman, Roger Ruess, and Trish Wurtz for participation in field work. NR 65 TC 0 Z9 0 U1 11 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0012-9658 EI 1939-9170 J9 ECOLOGY JI Ecology PD NOV PY 2016 VL 97 IS 11 BP 2986 EP 2997 DI 10.1002/ecy.1529 PG 12 WC Ecology SC Environmental Sciences & Ecology GA EB2YJ UT WOS:000387228200010 ER PT J AU Healey, SP Raymond, CL Lockman, IB Hernandez, AJ Garrard, C Huang, CQ AF Healey, Sean P. Raymond, Crystal L. Lockman, I. Blakey Hernandez, Alexander J. Garrard, Chris Huang, Chengquan TI Root disease can rival fire and harvest in reducing forest carbon storage SO ECOSPHERE LA English DT Article DE carbon; forest disturbance; forest management; forest pathology; root disease ID MOUNTAIN PINE-BEETLE; CLIMATE-CHANGE; UNITED-STATES; NATURAL DISTURBANCES; BRITISH-COLUMBIA; CONIFER FORESTS; DYNAMICS; IMPACT; USA; INVENTORY AB Root diseases are known to suppress forest regeneration and reduce growth rates, and they may become more common as susceptible tree species become maladapted in parts of their historic ranges due to climate change. However, current ecosystem models do not track the effects of root disease on net productivity, and there has been little research on how the dynamics of root disease affect carbon (C) storage and productivity across infected landscapes. We compared the effects of root disease against the effects of other types of forest disturbance across six national forest landscapes, 1990-2011. This was enabled by a monitoring tool called the Forest Carbon Management Framework (ForCaMF), which makes use of ground inventory data, an empirical growth model, and time series of Landsat satellite imagery. Despite several large fires that burned across these landscapes during the study period, retrospective ForCaMF analysis showed that fire and root disease had approximately equal impacts on C storage. Relative to C accumulation that would have occurred in their absence, fires from 1990 to 2011 were estimated to reduce regionwide C storage by 215.3 +/- 19.1 g/m(2) C, while disease in the same period was estimated to reduce storage by 211.4 +/- 59.9 g/m(2) C. Harvest (75.5 +/- 13.5 g/m(2) C) and bark beetle activity (14.8 +/- 12.5 g/m(2) C) were less important. While long-term disturbance processes such as root disease have generally been ignored by tools informing management of forest C storage, the recent history of several national forests suggests that such disturbances can be just as important to the C cycle as more conspicuous events like wildfires. C1 [Healey, Sean P.] US Forest Serv, Rocky Mt Res Stn, 507 25th St, Ogden, UT 84401 USA. [Raymond, Crystal L.] Seattle City Light, 700 5th Ave, Seattle, WA 98124 USA. [Lockman, I. Blakey] US Forest Serv State & Private Forestry, 200 East Broadway, Missoula, MT 59807 USA. [Hernandez, Alexander J.; Garrard, Chris] Utah State Univ, Coll Nat Resources, 5275 Old Main Hill, Logan, UT 84322 USA. [Huang, Chengquan] Univ Maryland, Dept Geog Sci, 1165 LeFrak Hall, College Pk, MD 20742 USA. RP Healey, SP (reprint author), US Forest Serv, Rocky Mt Res Stn, 507 25th St, Ogden, UT 84401 USA. EM seanhealey@fs.fed.us FU Forest Service Office of Sustainability and Climate Change; FIA program; NFS Northern Region Resource Information Management Board; NASA's Applied Science Program [NNH11AQ97I] FX The authors greatly appreciate the expertise and advice offered by members of the NFS Northern Regional Office, particularly Jim Morrison, Renate Bush, Barry Bollenbacher, and Keith Stockmann. Alicia Peduzzi of the Northern Research Station and Don Vandendriesche of the NFS Southwestern Region provided substantial help with FVS modeling. Liz Lapoint, Jim Menlove, Tracey Frescino, and Mike Thompson provided needed coaching in the interpretation of FIA data. Susan Hagle played an important role in the production of the root disease hazard map. This work was supported by the Forest Service Office of Sustainability and Climate Change, the FIA program, the NFS Northern Region Resource Information Management Board, and NASA's Applied Science Program (Grant No. NNH11AQ97I). NR 45 TC 0 Z9 0 U1 8 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD NOV PY 2016 VL 7 IS 11 AR e01569 DI 10.1002/ecs2.1569 PG 16 WC Ecology SC Environmental Sciences & Ecology GA EB2UI UT WOS:000387217700019 ER PT J AU Knapp, EE Ritchie, MW AF Knapp, Eric E. Ritchie, Martin W. TI Response of understory vegetation to salvage logging following a high-severity wildfire SO ECOSPHERE LA English DT Article DE high-severity wildfire; non-native species; Pinus ponderosa; postfire management; salvage logging; species diversity; species richness; variable retention salvage ID PONDEROSA PINE FORESTS; WESTERN UNITED-STATES; COARSE WOODY DEBRIS; MIXED-CONIFER FORESTS; CAVITY-NESTING BIRDS; SIERRA-NEVADA; NORTHEASTERN OREGON; NORTHERN ARIZONA; FIRE SEVERITY; SOUTHERN CASCADES AB Timber is frequently salvage-logged following high-severity stand-replacing wildfire, but the practice is controversial. One concern is that compound disturbances could result in more deleterious impacts than either disturbance individually, with mechanical operations having the potential to set back recovering native species and increase invasion by non-native species. Following the 2002 Cone Fire on the Lassen National Forest, three replicates of five salvage treatments were applied to 15 units formerly dominated by ponderosa pine, covering a range of disturbance intensities from unsalvaged to 100% salvaged. Understory species richness and cover data were collected every two years between 2006 and 2012. Richness of both native and non-native species did not differ among salvage treatments, but both showed strong changes over time. While cover of forbs and graminoids did not differ with salvage treatment, cover of shrubs was significantly reduced at the higher salvage intensities. The three main shrub species are all stimulated to germinate by fire, potentially leaving seedlings vulnerable to any mechanical disturbance occurring immediately postgermination. Many other native perennial species emerge from rhizomes or other deeply buried underground structures and appear to be less affected by salvage harvest. Over time, the plant community in all salvage treatments shifted from dominance by shrubs and forbs to shrubs and grasses. Most of the grasses were native, except Bromus tectorum (cheatgrass), which was found in 4% of measurement quadrats in 2006 and 52% in 2012. Our results indicated that understory vegetation change 4-10 years post-high-severity wildfire appeared to be influenced more strongly by factors other than salvage logging. C1 [Knapp, Eric E.; Ritchie, Martin W.] US Forest Serv, USDA, Pacific Southwest Res Stn, 3644 Avtech Pkwy, Redding, CA 96002 USA. RP Knapp, EE (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, 3644 Avtech Pkwy, Redding, CA 96002 USA. EM eknapp@fs.fed.us FU Joint Fire Science Program grant [07-2-2-09] FX Justin Crotteau, Todd Hamilton, Ron Saleme, Travis Springer, Ryan Tony, Hai Vuong, Brett Watson, and Brian Wing collected much of the field data. Don Lepley, botanist on the Lassen NF, helped with plant identification, Celeste Abbott and Terrie Alves helped to summarize data, Jim Baldwin assisted with the statistical analyses, and Carl Skinner provided feedback on an earlier version of the manuscript. Data collection was funded, in part, by the Joint Fire Science Program grant 07-2-2-09. NR 81 TC 0 Z9 0 U1 11 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD NOV PY 2016 VL 7 IS 11 AR e01550 DI 10.1002/ecs2.1550 PG 17 WC Ecology SC Environmental Sciences & Ecology GA EB2UI UT WOS:000387217700012 ER PT J AU Riley, KL Loehman, RA AF Riley, Karin L. Loehman, Rachel A. TI Mid-21st-century climate changes increase predicted fire occurrence and fire season length, Northern Rocky Mountains, United States SO ECOSPHERE LA English DT Article DE burn probability; climate change; climatic fire season length; fire occurrence; FSim; Northern Rocky Mountains ID WILDLAND FIRE; VEGETATION DYNAMICS; FOREST STRUCTURE; WILDFIRE; USA; REGIMES; MANAGEMENT; DANGER; MODELS; SCALE AB Climate changes are expected to increase fire frequency, fire season length, and cumulative area burned in the western United States. We focus on the potential impact of mid-21st-century climate changes on annual burn probability, fire season length, and large fire characteristics including number and size for a study area in the Northern Rocky Mountains. Although large fires are rare they account for most of the area burned in western North America, burn under extreme weather conditions, and exhibit behaviors that preclude methods of direct control. Allocation of resources, development of management plans, and assessment of fire effects on ecosystems all require an understanding of when and where fires are likely to burn, particularly under altered climate regimes that may increase large fire occurrence. We used the large fire simulation model FSim to model ignition, growth, and containment of wildfires under two climate scenarios: contemporary (based on instrumental weather) and mid-century (based on an ensemble average of global climate models driven by the A1B SRES emissions scenario). Modeled changes in fire patterns include increased annual burn probability, particularly in areas of the study region with relatively short contemporary fire return intervals; increased individual fire size and annual area burned; and fewer years without large fires. High fire danger days, represented by threshold values of Energy Release Component (ERC), are projected to increase in number, especially in spring and fall, lengthening the climatic fire season. For fire managers, ERC is an indicator of fire intensity potential and fire economics, with higher ERC thresholds often associated with larger, more expensive fires. Longer periods of elevated ERC may significantly increase the cost and complexity of fire management activities, requiring new strategies to maintain desired ecological conditions and limit fire risk. Increased fire activity (within the historical range of frequency and severity, and depending on the extent to which ecosystems are adapted) may maintain or restore ecosystem functionality; however, in areas that are highly departed from historical fire regimes or where there is disequilibrium between climate and vegetation, ecosystems may be rapidly and persistently altered by wildfires, especially those that burn under extreme conditions. C1 [Riley, Karin L.] US Forest Serv, Forestry Sci Lab, USDA, Rocky Mt Res Stn, 800 East Beckwith, Missoula, MT 59801 USA. [Loehman, Rachel A.] US Geol Survey, Alaska Sci Ctr, 4210 Univ Dr, Anchorage, AK 99508 USA. RP Riley, KL (reprint author), US Forest Serv, Forestry Sci Lab, USDA, Rocky Mt Res Stn, 800 East Beckwith, Missoula, MT 59801 USA. EM kriley@fs.fed.us FU Region 1 of the U.S. Forest Service FX We thank Jessica Haas for in-depth discussions that contributed to the methods presented here. Region 1 of the U.S. Forest Service provided partial funding for the project. We appreciate comments by Dr. Faith Ann Heinsch and two anonymous reviewers who helped improve this manuscript. Any use of trade names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 81 TC 0 Z9 0 U1 20 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD NOV PY 2016 VL 7 IS 11 AR e01543 DI 10.1002/ecs2.1543 PG 19 WC Ecology SC Environmental Sciences & Ecology GA EB2UI UT WOS:000387217700007 ER PT J AU Monroe, MC Ballard, HL Oxarart, A Sturtevant, VE Jakes, PJ Evans, ER AF Monroe, Martha C. Ballard, Heidi L. Oxarart, Annie Sturtevant, Victoria E. Jakes, Pamela J. Evans, Emily R. TI Agencies, educators, communities and wildfire: partnerships to enhance environmental education for youth SO ENVIRONMENTAL EDUCATION RESEARCH LA English DT Article DE community; agency; wildfire; educators; partnership ID ORGANIZATIONS; COLLABORATION; PERSPECTIVES AB We studied seven programs that engage youth from 10 to 18years old in wildfire risk reduction in their communities in the United States through in-depth interviews to examine the nature and role of community-school partnerships in resource-focused environmental education. While the programs use a variety of strategies, from Scout badge to summer school, they exhibit several common dimensions: they all engage youth in community projects; they all arise from partnerships between resource agencies, community organizations, and educators; they all began when people familiar with both wildfire and youth education saw an opportunity to improve the community and educate youth through action; and all partners are able to contribute to the common program yet retain their individual identity as they meet their own mission-based goals. We use themes and quotes to illustrate these common dimensions for establishing community-school partnerships that could build action competence through environment-based education projects. C1 [Monroe, Martha C.] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [Oxarart, Annie] Univ Florida, Sch Forest Resources & Conservat, Several Extens Programs, Gainesville, FL 32611 USA. [Ballard, Heidi L.; Evans, Emily R.] Univ Calif Davis, Sch Educ, Davis, CA 95616 USA. [Sturtevant, Victoria E.] Southern Oregon Univ, Dept Sociol, Ashland, OR USA. [Jakes, Pamela J.] US Forest Serv, USDA, Northern Res Stn, St Paul, MN USA. RP Monroe, MC (reprint author), Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. EM mcmonroe@ufl.edu FU US Forest Service, Northern Research Station [10-JV-11,242,309-069] FX We are grateful for the willingness and dedication of the leaders of the seven youth wildfire programs who welcomed us as we conducted interviews and learned about their activities. The four anonymous reviewers of this draft manuscript offered extremely valuable comments and suggestions. We also thank the US Forest Service, Northern Research Station, for their financial support of this joint venture [10-JV-11,242,309-069]. NR 41 TC 0 Z9 0 U1 4 U2 4 PU ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD PI ABINGDON PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 1350-4622 EI 1469-5871 J9 ENVIRON EDUC RES JI Environ. Educ. Res. PD NOV PY 2016 VL 22 IS 8 BP 1098 EP 1114 DI 10.1080/13504622.2015.1057555 PG 17 WC Education & Educational Research; Environmental Studies SC Education & Educational Research; Environmental Sciences & Ecology GA EB1US UT WOS:000387141700002 ER PT J AU Sullivan, PF Pattison, RR Brownlee, AH Cahoon, SMP Hollingsworth, TN AF Sullivan, Patrick F. Pattison, Robert R. Brownlee, Annalis H. Cahoon, Sean M. P. Hollingsworth, Teresa N. TI Effect of tree-ring detrending method on apparent growth trends of black and white spruce in interior Alaska SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE boreal; dendrochronology; detrending; drought; Picea glauca; Picea mariana ID CLIMATE VARIABILITY; CRUST SOFTWARE; BIOME SHIFT; FORESTS; STANDARDIZATION; RCS; IMPLEMENTATION; CONSISTENT; PROGRAM AB Boreal forests are critical sinks in the global carbon cycle. However, recent studies have revealed increasing frequency and extent of wildfires, decreasing landscape greenness, increasing tree mortality and declining growth of black and white spruce in boreal North America. We measured ring widths from a large set of increment cores collected across a vast area of interior Alaska and examined implications of data processing decisions for apparent trends in black and white spruce growth. We found that choice of detrending method had important implications for apparent long-term growth trends and the strength of climate-growth correlations. Trends varied from strong increases in growth since the Industrial Revolution, when ring widths were detrended using single-curve regional curve standardization (RCS), to strong decreases in growth, when ring widths were normalized by fitting a horizontal line to each ring width series. All methods revealed a pronounced growth peak for black and white spruce centered near 1940. Most detrending methods showed a decline from the peak, leaving recent growth of both species near the long-term mean. Climate-growth analyses revealed negative correlations with growing season temperature and positive correlations with August precipitation for both species. Multiple-curve RCS detrending produced the strongest and/or greatest number of significant climate-growth correlations. Results provide important historical context for recent growth of black and white spruce. Growth of both species might decline with future warming, if not mitigated by increasing precipitation. However, widespread drought-induced mortality is probably not imminent, given that recent growth was near the long-term mean. C1 [Sullivan, Patrick F.; Brownlee, Annalis H.; Cahoon, Sean M. P.] Univ Alaska Anchorage, Environm & Nat Resources Inst, Anchorage, AK 99508 USA. [Pattison, Robert R.] US Forest Serv, Pacific Northwest Res Stn, USDA, Anchorage, AK 99503 USA. [Hollingsworth, Teresa N.] US Forest Serv, Boreal Cooperat Res Unit, USDA, Fairbanks, AK 99775 USA. RP Sullivan, PF (reprint author), Univ Alaska Anchorage, Environm & Nat Resources Inst, Anchorage, AK 99508 USA. EM pfsullivan@alaska.edu FU Pacific Northwest Research Station (PNWRS); NASA Carbon Monitoring System (CMS) grant; PNWRS's Resource Monitoring Assessment Program; PNWRS [14-JV-11261919-030]; University of Alaska Anchorage [14-JV-11261919-030]; CMS FX This study was funded by two 'Focus Area' awards to RRP, TNH and H Andersen from the Pacific Northwest Research Station (PNWRS), by a NASA Carbon Monitoring System (CMS) grant and by the PNWRS's Resource Monitoring Assessment Program. Involvement of PFS, AHB and SMPC was funded through a joint venture agreement #14-JV-11261919-030 between the PNWRS and the University of Alaska Anchorage. J Hollingsworth and S Frost collected increment cores on the AIRIS plots. H Andersen, B Cook, D Morton, R Nelson and A Finley assisted in acquiring CMS funding. T Melvin and E Nicklen provided valuable comments on an early draft of the manuscript. NR 37 TC 0 Z9 0 U1 20 U2 20 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD NOV PY 2016 VL 11 IS 11 AR 114007 DI 10.1088/1748-9326/11/11/114007 PG 12 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA EB7JN UT WOS:000387563200001 ER PT J AU Shi, AN Mou, BQ Correll, JC AF Shi, Ainong Mou, Beiquan Correll, James C. TI Association analysis for oxalate concentration in spinach SO EUPHYTICA LA English DT Article DE Association mapping; Germplasm; Breeding; Genotyping by sequencing (GBS); Oxalate concentration; Single nucleotide polymorphism (SNP); Spinacia oleracea ID MARKER-ASSISTED SELECTION; GENOME-WIDE ASSOCIATION; SEQUENCING GBS; OXALIC-ACID; TRAITS; NITRATE; GERMPLASM; STRATIFICATION; COLLECTION; RESISTANCE AB Reducing oxalate content of spinach is a major breeding objective. The aim of this research was to conduct association analysis and identify SNP markers associated with oxalate concentration in spinach germplasm. A total of 310 spinach genotypes, including 300 USDA germplasm accessions and ten commercial cultivars, were used for the association analysis of oxalate concentration. Genotyping by sequencing was used to identify 841 SNPs among the genotypes examined for the association analysis. The distribution of oxalate concentration showed a near normal distribution with a wide range in concentrations from 647.2 to 1286.9 mg/100 g on a fresh weight basis and 53.4 to 108.8 mg/g on a dry weight basis. The range in oxalate concentration in spinach suggests that it is a complex quantitative trait which may be controlled by multiple genes, each with a minor effect among the tested spinach panel. Association analysis indicated that six SNP markers (AYZV02031464_116, AYZV02031464_117, AYZV02031464_95, AYZV02283363_2707, AYZV02287123_2830, and AYZV02296293_852) were associated with the oxalate concentration. The SNP markers may be useful for breeders to select germplasm for reduced oxalate concentrations in spinach breeding programs through marker-assisted selection. C1 [Shi, Ainong] Univ Arkansas, Dept Hort, Fayetteville, AR 72701 USA. [Mou, Beiquan] ARS, Crop Improvement & Protect Res Unit, USDA, 1636 E Alisal St, Salinas, CA 93905 USA. [Correll, James C.] Univ Arkansas, Dept Plant Pathol, Fayetteville, AR 72701 USA. RP Shi, AN (reprint author), Univ Arkansas, Dept Hort, Fayetteville, AR 72701 USA.; Mou, BQ (reprint author), ARS, Crop Improvement & Protect Res Unit, USDA, 1636 E Alisal St, Salinas, CA 93905 USA.; Correll, JC (reprint author), Univ Arkansas, Dept Plant Pathol, Fayetteville, AR 72701 USA. EM ashi@uark.edu; beiquan.mou@ars.usda.gov; jcorrell@uark.edu FU USDA National Institute of Food and Agriculture Hatch project [1002423] FX This work is supported, in part, by the USDA National Institute of Food and Agriculture Hatch project accession number 1002423. NR 57 TC 0 Z9 0 U1 9 U2 9 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD NOV PY 2016 VL 212 IS 1 BP 17 EP 28 DI 10.1007/s10681-016-1740-0 PG 12 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA EB4QP UT WOS:000387358200003 ER PT J AU McAllister, S Finney, M AF McAllister, Sara Finney, Mark TI Burning Rates of Wood Cribs with Implications for Wildland Fires SO FIRE TECHNOLOGY LA English DT Article DE Burning rate; Cribs; Porosity; Vertical gap; Wildland fire ID COMBUSTION CHARACTERISTICS; CONE CALORIMETER; VEGETATION; FUELS; TIMES AB Wood cribs are often used as ignition sources for room fire tests and the well characterized burning rates may also have applications to wildland fires. The burning rate of wildland fuel structures, whether the needle layer on the ground or trees and shrubs themselves, is not addressed in any operational fire model and no simple model exists. Several relations exist in the literature for the burning rate of wood cribs, but the cribs used to generate them were built with fairly limited geometries. This work explores the burning rate of cribs with a wide variety of geometries and aspect ratios in the loosely-packed regime to evaluate the rigor of several correlations from the literature. Specifically, stick thicknesses ranged from 0.16 cm to 1.27 cm and lengths from 6.4 cm to 61.0 cm resulting in aspect ratios (stick length/thickness) from 10 cm to 160. As wildland fuel beds occur both directly on the ground and suspended in the air, the effect of the vertical gap between the ground and crib base was also examined. The critical vertical gap was shown to be larger than previously thought (7.6 cm for all cribs) and a function of the aspect ratio. It was quite apparent that as the aspect ratio increases, a significant portion of the required oxidizer comes from the bottom of the crib. A relation is then found to adjust the predicted values for the reduction in burning rate due to insufficient vertical gap. C1 [McAllister, Sara; Finney, Mark] US Forest Serv, USDA, RMRS Missoula Fire Sci Lab, 5775 W US Highway 10, Missoula, MT 59808 USA. RP McAllister, S (reprint author), US Forest Serv, USDA, RMRS Missoula Fire Sci Lab, 5775 W US Highway 10, Missoula, MT 59808 USA. EM smcallister@fs.fed.us FU National Fire Decision Support Center FX The authors wish to thank James McGuire, Jennifer Kennedy, and Sophia Vernholm for their tireless and careful construction of the cribs and Cyle Wold for setting up the data acquisition system. Funding for this work was provided by the National Fire Decision Support Center. NR 40 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0015-2684 EI 1572-8099 J9 FIRE TECHNOL JI Fire Technol. PD NOV PY 2016 VL 52 IS 6 BP 1755 EP 1777 DI 10.1007/s10694-015-0543-5 PG 23 WC Engineering, Multidisciplinary; Materials Science, Multidisciplinary SC Engineering; Materials Science GA EB1IY UT WOS:000387105200007 ER PT J AU Kutyrev, I Cleveland, B Leeds, T Wiens, GD AF Kutyrev, Ivan Cleveland, Beth Leeds, Timothy Wiens, Gregory D. TI Proinflammatory cytokine and cytokine receptor gene expression kinetics following challenge with Flavobacterium psychrophilum in resistant and susceptible lines of rainbow trout (Oncorhynchus mykiss) SO FISH & SHELLFISH IMMUNOLOGY LA English DT Article DE Flavobacterium psychrophilum; Bacterial cold water disease; Genetic resistance; Selective breeding; Proinflammatory cytokines; Cytokine receptors ID SERUM-AMYLOID-A; SALMON SALMO-SALAR; COLD-WATER DISEASE; IMMUNE-RELEVANT GENES; ACUTE-PHASE RESPONSE; HEPATITIS-C VIRUS; ATLANTIC SALMON; TELEOST FISH; SPLEEN SIZE; SUBTRACTIVE HYBRIDIZATION AB Flavobacterium psychrophilum (Fp) is the causative agent of bacterial cold water disease (BCWD) which causes appreciable economic losses in rainbow trout aquaculture. We previously reported development of a genetic line, designated ARS-Fp-R that exhibits higher survival relative to a susceptible line, designated ARS-Fp-S, following either laboratory or natural on-farm challenge. The objectives of this study were to determine the temporal kinetics of gene expression between experimentally-challenged ARS-Fp-R and ARS-Fp-S fish and the correlation between gene expression and pathogen load. We developed a GeXP multiplex RT-PCR assay to simultaneously examine expression of immune-relevant genes, concentrating on tumor necrosis factor and interleukin-1 ligand/receptor systems and acute phase response genes. Spleen tissue was sampled at 6 h, 24 h, 48 h and 144 h post-challenge and pathogen load quantified by qPCR. Transcript abundance of cytokine genes tnfa1, tnfa2, tnfa3, il1b1, il1b2, il11a; acute phase response genes saa and drtp1; and putative cytokine receptors il1r1-like-b, il1r2, tnfrsf1a, tnfrsf9, tnfrsf1a-like-b increased following challenge while the transcript abundance of il1r-like-1 and tnfrsf1a-like-a decreased compared to PBS-injected line-matched control fish. Principal component analysis identified transcript levels of genes il1r-like-1 and tnfrsf1a-like-a as exhibiting differential expression between genetic lines. In summary, Fp i.p. injection challenge elicited a proinflammatory cytokine gene expression response in the spleen, with ARS-Fp-R line fish exhibiting modestly higher basal expression levels of several putative cytokine receptors. This study furthers the understanding of the immune response following Fp challenge and differences in gene expression associated with selective breeding for disease resistance. Published by Elsevier Ltd. C1 [Kutyrev, Ivan; Cleveland, Beth; Leeds, Timothy; Wiens, Gregory D.] ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, 11861 Leetown Rd, Kearneysville, WV 25430 USA. [Kutyrev, Ivan] Russian Acad Sci, Siberian Branch, Inst Gen & Expt Biol, Sakhyanovoi St 6, Ulan Ude 670047, Russia. RP Kutyrev, I; Wiens, GD (reprint author), ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, 11861 Leetown Rd, Kearneysville, WV 25430 USA. EM ivan.kutyrev@yahoo.com; greg.wiens@ars.usda.gov FU Agriculture and Food Research Initiative competitive from the USDA National Institute of Food and Agriculture [2012-67015-30217]; Agricultural Research Service CRIS Project "Integrated Research to Improve On-Farm Animal health in Salmonid Aqua-culture" [1930-32000-006]; Russian Fulbright Visiting Scholar Program; Russian Foundation for Basic Research [16-04-01213]; project of the Siberian Branch of the Russian Academy of Sciences [VI.51.1.3] FX The authors would like to thank Joel Caren and Keira Osbourn for assisting with sample and data collection and Dr. Gaungtu Gao for help with genome analyses. We thank Dr. P. Zwollo (College of Wiliam and Mary) for providing RNA used for initial primer testing. This work was supported by Agriculture and Food Research Initiative competitive grant no. 2012-67015-30217 from the USDA National Institute of Food and Agriculture and by Agricultural Research Service CRIS Project 1930-32000-006 "Integrated Research to Improve On-Farm Animal health in Salmonid Aqua-culture". Support was also received from the 2014-2015 Russian Fulbright Visiting Scholar Program awarded to IK and the Russian Foundation for Basic Research (project no. 16-04-01213). This study was performed under the project of the Siberian Branch of the Russian Academy of Sciences, VI.51.1.3. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S Department of Agriculture. USDA is an equal opportunity employer. NR 92 TC 0 Z9 0 U1 3 U2 3 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1050-4648 EI 1095-9947 J9 FISH SHELLFISH IMMUN JI Fish Shellfish Immunol. PD NOV PY 2016 VL 58 BP 542 EP 553 DI 10.1016/j.fsi.2016.09.053 PG 12 WC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences GA EB6VG UT WOS:000387523100064 PM 27693200 ER PT J AU Atherly, T Mosher, C Wang, C Hostetter, J Proctor, A Brand, MW Phillips, GJ Wannemuehler, M Jergens, AE AF Atherly, Todd Mosher, Curtis Wang, Chong Hostetter, Jesse Proctor, Alexandra Brand, Meghan W. Phillips, Gregory J. Wannemuehler, Michael Jergens, Albert E. TI Helicobacter bilis Infection Alters Mucosal Bacteria and Modulates Colitis Development in Defined Microbiota Mice SO INFLAMMATORY BOWEL DISEASES LA English DT Article DE Helicobacter bilis; colitis; altered Schaedler flora; IBD; fluorescence in situ hybridization ID INFLAMMATORY-BOWEL-DISEASE; MOLECULAR-PHYLOGENETIC CHARACTERIZATION; IN-SITU HYBRIDIZATION; HEPATICUS INFECTION; COMMENSAL BACTERIA; SCHAEDLER FLORA; CROHNS-DISEASE; IMMUNE REACTIVITY; ESCHERICHIA-COLI; DEFICIENT MICE AB Background:Helicobacter bilis infection of C3H/HeN mice harboring the altered Schaedler flora (ASF) triggers progressive immune responsiveness and the development of colitis. We sought to investigate temporal alterations in community structure of a defined (ASF-colonized) microbiota in normal and inflamed murine intestines and to correlate microbiota changes to histopathologic lesions.Methods:The colonic mucosal microbiota of healthy mice and ASF mice colonized with H. bilis for 3, 6, or 12 weeks were investigated by fluorescence in situ hybridization targeting the 16S ribosomal RNA genes of total bacteria, group-specific organisms, and individual ASF bacterial species. Microbial profiling of ASF and H. bilis abundance was performed on cecal contents.Results:Helicobacter bilis-colonized mice developed colitis associated with temporal changes in composition and spatial distribution of the mucosal microbiota. The number of total bacteria, ASF519, and helicobacter-positive bacteria were increased (P < 0.05), whereas ASF360/361-positive bacteria were decreased (P < 0.05) versus controls. Adherent biofilms in colitic mice were most often (P < 0.05) composed of total bacteria, ASF457, and H. bilis. Total numbers of ASF519 and H. bilis bacteria were positively correlated (P = 0.03, r = 0.39 and P < 0.0001, r = 0.73), and total numbers of ASF360/361 bacteria were negatively correlated (P = 0.003, r = -0.53) to histopathologic score. Differences in cecal abundance of ASF members were not observed.Conclusions:Altered community structure with murine colitis is characterized by distinct ASF bacteria that interact with the colonic mucosa, by formation of an isolating interlaced layer, by attachment, or by invasion, and this interaction is differentially expressed over time. C1 [Atherly, Todd] USDA ARS, Ames, IA 50011 USA. [Mosher, Curtis] Iowa State Univ, Dept Genet Dev & Cell Biol, Coll Liberal Arts & Sci, Ames, IA 50011 USA. [Wang, Chong] Iowa State Univ, Dept Vet Diagnost & Prod Anim Med, Ames, IA 50011 USA. [Hostetter, Jesse] Iowa State Univ, Dept Vet Pathol, Ames, IA 50011 USA. [Proctor, Alexandra; Brand, Meghan W.; Phillips, Gregory J.; Wannemuehler, Michael] Iowa State Univ, Dept Veterinary Microbiol & Prevent Med, Ames, IA 50011 USA. [Jergens, Albert E.] Iowa State Univ, Coll Vet Med, Dept Vet Clin Sci, Ames, IA USA. RP Jergens, AE (reprint author), Iowa State Univ, Coll Vet Med, Dept Vet Clin Sci, Ames, IA USA. EM ajergens@iastate.edu FU NCRR NIH HHS [K01 RR018618]; NIGMS NIH HHS [R01 GM099537] NR 50 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 1078-0998 EI 1536-4844 J9 INFLAMM BOWEL DIS JI Inflamm. Bowel Dis. PD NOV PY 2016 VL 22 IS 11 BP 2571 EP 2581 DI 10.1097/MIB.0000000000000944 PG 11 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA EB3FK UT WOS:000387248500011 PM 27755267 ER PT J AU Good, C May, T Crouse, C Summerfelt, S Welch, TJ AF Good, C. May, T. Crouse, C. Summerfelt, S. Welch, T. J. TI Assessing the impact of swimming exercise and the relative susceptibility of rainbow trout Oncorhynchus mykiss (Walbaum) and Atlantic salmon Salmo salar L. following injection challenge with Weissella ceti SO JOURNAL OF FISH DISEASES LA English DT Article DE Salmonids; swimming exercise; Weissellosis ID PERFORMANCE; INFECTION; MORTALITY; ENZYMES C1 [Good, C.; May, T.; Crouse, C.] Conservat Funds Freshwater Inst, 1098 Turner Rd, Shepherdstown, WV 25443 USA. [Welch, T. J.] ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, 11861 Leetown Rd, Kearneysville, WV 25430 USA. RP Good, C (reprint author), Conservat Funds Freshwater Inst, 1098 Turner Rd, Shepherdstown, WV 25443 USA.; Welch, TJ (reprint author), ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, 11861 Leetown Rd, Kearneysville, WV 25430 USA. EM c.good@freshwaterinstitute.org; tim.welch@ars.usda.gov FU USDA Agricultural Research Service (ARS) [59-1930-5-510] FX This research was funded by the USDA Agricultural Research Service (ARS) under Agreement No. 59-1930-5-510. Appreciation is extended to Jennifer Lipscomb (USDA-ARS-NCCCWA) for conducting the molecular analyses and to Travis Moreland (USDA-ARS-NCCCWA) for care of the research animals. The authors do not have any conflicts of interest to declare. Experimental protocols described are in compliance with the Animal Welfare Act (9CFR) and were approved by our Institutional Animal Care and Use Committee (IACUC). Use of trade names does not imply endorsement by the US Government. NR 14 TC 0 Z9 0 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7775 EI 1365-2761 J9 J FISH DIS JI J. Fish Dis. PD NOV PY 2016 VL 39 IS 11 BP 1387 EP 1391 DI 10.1111/jfd.12468 PG 5 WC Fisheries; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Marine & Freshwater Biology; Veterinary Sciences GA EB1KI UT WOS:000387109700011 PM 27087506 ER PT J AU Zerofsky, MS Jacoby, BN Pedersen, TL Stephensen, CB AF Zerofsky, Melissa S. Jacoby, Bryon N. Pedersen, Theresa L. Stephensen, Charles B. TI Daily Cholecalciferol Supplementation during Pregnancy Alters Markers of Regulatory Immunity, Inflammation, and Clinical Outcomes in a Randomized Controlled Trial SO JOURNAL OF NUTRITION LA English DT Article DE clinical trial; vitamin D; pregnancy; cytokines; T cell ID VITAMIN-D SUPPLEMENTATION; 25-HYDROXYVITAMIN D CONCENTRATIONS; GESTATIONAL DIABETES-MELLITUS; D DEFICIENCY; T-CELLS; BLOOD-PRESSURE; BIRTH-WEIGHT; DOUBLE-BLIND; RISK; SERUM AB Background: Vitamin D deficiency is widespread in pregnancy and has been associated with adverse health conditions in mothers and infants. Vitamin D supplementation in pregnancy may support the maintenance of pregnancy by its effects on innate and adaptive immunity. Objective: We assessed the effects of vitamin D supplementation during pregnancy on vitamin D status and markers of immune function associated with adverse pregnancy outcomes. Methods: We conducted a randomized, controlled, double-blind intervention of 2 doses of cholecalciferol (400 and 2000 IU/d) from <20 wk to delivery in 57 pregnant women. Vitamin D status, regulatory and inflammatory T cells, markers of innate immunity and systemic inflammation, and clinical outcomes including maternal blood pressure and birth weight were assessed at 26 and 36 wk of pregnancy. Results: Supplementation with 2000 IU/d vitamin D had a greater effect on the change in vitamin D status over pregnancy (P < 0.0001) and the final value at 36 wk (P <0.0001) than 400 IU/d, increasing serum 25-hydroxyvitamin D from 81.1 nmol/L at baseline to 116 nmol/L at 36 wk and from 69.6 nmol/L at baseline to 85.6 nmol/L at 36 wk, respectively. The 2000-IU/d group had 36% more interleukin-10(+) regulatory CD4(+) T cells at 36 wk than did the 400-IU/d group (P <0.007). The daily intake of 2000 compared with 400 IU/d tended to dampen the pregnancy-related increase in diastolic blood pressure by 1.3-fold (P = 0.06) and increase birth weight by 8.6% (P = 0.06), but these differences were not statistically significant. Conclusions: Supplementation with 2000 IU/d is more effective at increasing vitamin D status in pregnant women than 400 IU/d and is associated with increased regulatory T cell immunity that may prevent adverse outcomes caused by excess inflammation. This trial was registered at clinicaltrials.gov as NCT01417351. C1 [Zerofsky, Melissa S.] Univ Calif Davis, Davis Med Ctr, Grad Grp Nutr Biol, Davis, CA 95616 USA. [Jacoby, Bryon N.] Univ Calif Davis, Davis Med Ctr, Div Maternal Fetal Med, Davis, CA 95616 USA. [Zerofsky, Melissa S.; Pedersen, Theresa L.; Stephensen, Charles B.] USDA, Western Human Nutr Res Ctr, Davis, CA USA. [Jacoby, Bryon N.] Shenandoah Valley Maternal Fetal Med, 1840 Amherst St, Winchester, VA 22601 USA. RP Zerofsky, MS (reprint author), Univ Calif Davis, Davis Med Ctr, Grad Grp Nutr Biol, Davis, CA 95616 USA.; Zerofsky, MS (reprint author), USDA, Western Human Nutr Res Ctr, Davis, CA USA. EM mszerofsky@ucdavis.edu FU USDA; University of California; Davis Clinical and Translational Science Center [UL1 TR000002]; NOW Foods FX Supported by the USDA, University of California, Davis Clinical and Translational Science Center grant UL1 TR000002, and NOW Foods. NR 73 TC 2 Z9 2 U1 2 U2 2 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD NOV PY 2016 VL 146 IS 11 BP 2388 EP 2397 DI 10.3945/jn.116.231480 PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA EB3XD UT WOS:000387301500028 PM 27655755 ER PT J AU Schap, TE Kuczynski, KJ Ciampo, MA Chang, SA AF Schap, TusaRebecca E. Kuczynski, Kevin J. Ciampo, Melissa A. Chang, Sarah A. TI Food Choices: Small Shifts, Large Gains SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Editorial Material C1 [Schap, TusaRebecca E.; Kuczynski, Kevin J.; Ciampo, Melissa A.; Chang, Sarah A.] USDA, Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. RP Schap, TE (reprint author), USDA, Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. NR 2 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD NOV PY 2016 VL 116 IS 11 BP 1747 EP 1749 DI 10.1016/j.jand.2016.08.017 PG 3 WC Nutrition & Dietetics SC Nutrition & Dietetics GA EB2OZ UT WOS:000387203000003 PM 27788766 ER PT J AU Widaman, AM Witbracht, MG Forester, SM Laugero, KD Keim, NL AF Widaman, Adrianne M. Witbracht, Megan G. Forester, Shavawn M. Laugero, Kevin D. Keim, Nancy L. TI Chronic Stress Is Associated with Indicators of Diet Quality in Habitual Breakfast Skippers SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article DE Breakfast skipping; Stress; Healthy Eating Index; Food intake; Night-time eating ID ENERGY-BALANCE; FOOD-INTAKE; METABOLIC SYNDROME; AMERICAN ADULTS; YOUNG-ADULTS; HEALTH; CONSUMPTION; ADEQUACY; INSULIN; INDEX AB Background Previous studies suggest skipping breakfast is associated with lower diet quality, but possible reasons underlying this relationship are not clear. Objective Our aim was to determine the relationship between chronic stress and variations in diet quality in the context of breakfast eating or breakfast skipping. Design Based on morning eating habits, 40 breakfast eaters and 35 breakfast skippers participated in a cross-sectional study. Diet assessment was based on unannounced 24-hour recalls. Participants/setting Women, ages 18 to 45 years; with a body mass index (calculated as kg/m(2)) <40 were recruited in the greater Sacramento, CA, area between 2009 and 2013. Only women who consistently ate or skipped breakfast were enrolled. Main outcome measures Compliance with the 2010 Dietary Guidelines for Americans was measured using the Healthy Eating Index 2010 (HEI-2010). Stress and executive function were evaluated with validated questionnaires and a computer-based task, respectively. Statistical analyses performed Diet characteristics of breakfast eating and breakfast skipping were evaluated as nutrient densities (amounts per 1,000 kcal) and compared using a one-way analysis of covariance, with body mass index as covariate. Diet and stress variable associations were assessed using Pearson correlations. Results Despite no observed differences in daily energy intake between breakfast skipping and breakfast eating, overall diet quality (P=0.001), whole grains (P=0.002), fruit (P=0.002), empty calories (P=0.050), fiber (P=0.001), calcium (P=0.001), potassium (P=0.033), and folate (P=0.013) intakes were higher in breakfast eating. In the evening, breakfast skipping consumed more added sugars (P=0.012) and saturated fat (P=0.006). In breakfast skipping, reported stress was associated with empty calories (r=-0.39; P=0.027) and evening intake of added sugars (r=0.501: P=0.005). These relationships were not observed in breakfast eating. Conclusions Breakfast skippers were less likely to meet the Dietary Guidelines for Americans and consumed more empty calories at night. Chronic stress was related to evening eating choices and overall empty calories in the diet of breakfast skippers, whereas breakfast eaters' dietary intake did not appear to be affected by chronic stress. C1 [Widaman, Adrianne M.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Widaman, Adrianne M.; Witbracht, Megan G.] Univ Calif Davis, Davis, CA 95616 USA. [Witbracht, Megan G.] Univ Calif Irvine, Mind Res Unit, Irvine, CA USA. [Forester, Shavawn M.] Tahoe Forest Hosp, Truckee, CA USA. [Laugero, Kevin D.; Keim, Nancy L.] ARS, USDA, Western Human Nutr Res Ctr, 430 W Hlth Sci Dr, Davis, CA 95616 USA. RP Keim, NL (reprint author), ARS, USDA, Western Human Nutr Res Ctr, 430 W Hlth Sci Dr, Davis, CA 95616 USA. EM nancy.keim@ars.usda.gov FU ARS CRIS Projects [5306-51530-019-00D, 2032-51530-002-00D]; National Research Initiative Grant from the US Department of Agriculture's National Institute of Food and Agriculture, Human Nutrition and Obesity Program-31.5 [2009-35215-05364] FX Project supported by the National Research Initiative Grant #2009-35215-05364 from the US Department of Agriculture's National Institute of Food and Agriculture, Human Nutrition and Obesity Program-31.5 and ARS CRIS Projects 5306-51530-019-00D and 2032-51530-002-00D. The US Department of Agriculture is an equal opportunity provider and employer. NR 48 TC 0 Z9 0 U1 10 U2 10 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD NOV PY 2016 VL 116 IS 11 BP 1776 EP 1784 DI 10.1016/j.jand.2016.03.016 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA EB2OZ UT WOS:000387203000007 PM 27161025 ER PT J AU Costello, RB Dwyer, JT Saldanha, L Bailey, RL Merkel, J Wambogo, E AF Costello, Rebecca B. Dwyer, Johanna T. Saldanha, Leila Bailey, Regan L. Merkel, Joyce Wambogo, Edwina TI Do Cinnamon Supplements Have a Role in Glycemic Control in Type 2 Diabetes? A Narrative Review SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Review DE Cinnamon; Fasting plasma glucose; Hemoglobin A1c; Type 2 diabetes mellitus ID FASTING BLOOD-GLUCOSE; 3T3-L1 ADIPOCYTES; CLINICAL-TRIAL; EXTRACT; INSULIN; METAANALYSIS; HEMOGLOBIN; SAFETY; METABOLISM; COUMARIN AB Cinnamon (Cinnamomum sp) has been suggested to help patients with type 2 diabetes mellitus (T2DM) achieve better glycemic control, although conclusions from meta analyses are mixed. To evaluate whether the use of cinnamon dietary supplements by adults with T2DM had clinically meaningful effects on glycemic control, as measured by changes in fasting plasma glucose (FPG) or hemoglobin A1c (HbA1c), a comprehensive PubMed literature search was performed. Eleven randomized controlled trials were identified that met our inclusion criteria that enrolled 694 adults with T2DM receiving hypoglycemic medications or not. In 10 of the studies, participants continued to take their hypoglycemic medications during the cinnamon intervention period. Studies ranged from 4 to 16 weeks in duration; seven studies were double-blind. Cinnamon doses ranged from 120 to 6,000 mg/day. The species of cinnamon used varied: seven used Cinnamomum cassia or Cinnamomum aromaticum, one used Cinnamomum zeylanicum, and three did not disclose the species. Because of the heterogeneity of the studies, a meta-analysis was not conducted. All 11 of the studies reported some reductions in FPG during the cinnamon intervention, and of the studies measuring HbA1c very modest decreases were also apparent with cinnamon, whereas changes in the placebo groups were minimal. However, only four studies achieved the American Diabetes Association treatment goals (FPG <7.2 mmol/L [130 mg/dL] and/or HbAlc <7.0). We conclude that cinnamon supplements added to standard hypoglycemic medications and other lifestyle therapies had modest effects on FPG and HbA1c. Until larger and more rigorous studies are available, registered dietitian nutritionists and other health care professionals should recommend that patients continue to follow existing recommendations of authoritative bodies for diet, lifestyle changes, and hypoglycemic drugs. C1 [Costello, Rebecca B.; Dwyer, Johanna T.; Saldanha, Leila; Bailey, Regan L.; Merkel, Joyce; Wambogo, Edwina] NIH, Off Dietary Supplements, 6100 Execut Blvd,Suite 3B01, Bethesda, MD 20892 USA. [Dwyer, Johanna T.] Tufts Univ, Sch Med, Nutr, Boston, MA 02111 USA. [Dwyer, Johanna T.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Dwyer, Johanna T.] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Ctr Aging, Boston, MA 02111 USA. [Bailey, Regan L.] Purdue Univ, Dept Nutr Sci, W Lafayette, IN 47907 USA. RP Costello, RB (reprint author), NIH, Off Dietary Supplements, 6100 Execut Blvd,Suite 3B01, Bethesda, MD 20892 USA. EM costellb@od.nih.gov FU Office of Dietary Supplements of the National Institutes of Health, Department of Health and Human Services; US Department of Agriculture, Agricultural Research Service [58-1950-0-014] FX This work was supported by the Office of Dietary Supplements of the National Institutes of Health, Department of Health and Human Services. Partial support was also provided by the US Department of Agriculture, Agricultural Research Service, under agreement no. 58-1950-0-014. NR 50 TC 1 Z9 1 U1 13 U2 13 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD NOV PY 2016 VL 116 IS 11 BP 1794 EP 1802 DI 10.1016/j.jand.2016.07.015 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA EB2OZ UT WOS:000387203000009 PM 27618575 ER PT J AU Rahmani, MS Pijut, PM Shabanian, N AF Rahmani, Mohammad-Shafie Pijut, Paula M. Shabanian, Naghi TI Protoplast isolation and genetically true-to-type plant regeneration from leaf- and callus-derived protoplasts of Albizia julibrissin SO PLANT CELL TISSUE AND ORGAN CULTURE LA English DT Article DE Albizia; Cellulase; CPW; Genetic fidelity; Microcolony formation; Protoplast; Silk tree ID VITRO SHOOT FORMATION; MESOPHYLL PROTOPLASTS; MOLECULAR CHARACTERIZATION; SUSPENSION-CULTURES; SCION IMPROVEMENT; SATSUMA MANDARIN; SOMATIC HYBRIDS; EXCISED ROOTS; SWEET ORANGE; ISSR AB Protoplast isolation and subsequent plant regeneration of Albizia julibrissin was achieved from leaf and callus explants. Leaf tissue from 4 to 5-week-old in vitro seedlings was the best source for high-yield protoplast isolation. This approach produced 7.77 x 10(5) protoplasts (Pp) per gram fresh weight with 94 % viability; after 60 min pre-plasmolysis with 0.7 M sorbitol followed by digestion in a solution of cell and protoplast wash plus 0.7 M mannitol, 1.5 % cellulase Onozuka R10, and 1 % pectolyase Y-23 for 6 h. Liquid Kao and Michayluk medium containing 2.7 mu M alpha-naphthaleneacetic acid (NAA) and 2.2 mu M 6-benzylaminopurine (BA) was best for sustained cell division and microcolony formation from both leaf- and callus-derived protoplasts at a density of 3-5 x 10(5) Pp ml(-1). Protoplast-derived microcalli became visible after 3-4 weeks on semi-solid medium of the same composition. Microcalli were then cultured on Murashige and Skoog (MS) medium containing Gamborg B5 vitamins or woody plant medium supplemented with different concentrations of NAA plus 4.4 mu M BA for further growth. Proliferated leaf- and callus-protoplast-derived calli differentiated into microshoots on MS medium containing 13.2 mu M BA plus 4.6 mu M zeatin after 2-3 weeks, with an overall shoot organogenesis efficiency of 78-93 %. Rooting of microshoots on half-strength MS medium containing 4.9 A mu M indole-3-butyric acid was successful, and plantlets were acclimatized to the greenhouse with a survival rate of > 62 %. Using ten start codon targeted and ten inter-simple sequence repeat primers, the genetic integrity of nine leaf- and six callus-protoplast-based plants was validated along with the mother seedlings. C1 [Rahmani, Mohammad-Shafie; Shabanian, Naghi] Univ Kurdistan, Fac Nat Resources, Dept Forestry, Lab Forest Tree Biol & Biotechnol, Khanagah Campus, Sanandaj 6617715175, Iran. [Pijut, Paula M.] US Forest Serv, USDA, Northern Res Stn, Hardwood Tree Improvement & Regenerat Ctr, 715 West State St, W Lafayette, IN 47907 USA. RP Shabanian, N (reprint author), Univ Kurdistan, Fac Nat Resources, Dept Forestry, Lab Forest Tree Biol & Biotechnol, Khanagah Campus, Sanandaj 6617715175, Iran.; Pijut, PM (reprint author), US Forest Serv, USDA, Northern Res Stn, Hardwood Tree Improvement & Regenerat Ctr, 715 West State St, W Lafayette, IN 47907 USA. EM ppijut@purdue.edu; n.shabanian@uok.ac.ir FU Ministry of Science, Research and Technology of Iran FX The authors gratefully acknowledge Drs. Scott Merkle and Praveen K. Saxena for their constructive review and suggestions for the improvement of this manuscript. Financial support for this research was funded by the Ministry of Science, Research and Technology of Iran for the University of Kurdistan. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that also may be suitable. NR 78 TC 0 Z9 0 U1 11 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6857 EI 1573-5044 J9 PLANT CELL TISS ORG JI Plant Cell Tissue Organ Cult. PD NOV PY 2016 VL 127 IS 2 BP 475 EP 488 DI 10.1007/s11240-016-1072-8 PG 14 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA EB2DT UT WOS:000387168500018 ER PT J AU Cook, D Gardner, DR Roper, JM Ransom, CV Pfister, JA Panter, KE AF Cook, Daniel Gardner, Dale R. Roper, Jessie M. Ransom, Corey V. Pfister, James A. Panter, Kip E. TI Fungicide treatment and clipping of Oxytropis sericea does not disrupt swainsonine concentrations SO TOXICON LA English DT Article DE Locoweed; Oxytropis; Swainsonine; Fungicide; Clipping ID FUNGAL ENDOPHYTES; ALKALOID SWAINSONINE; EMBELLISIA-OXYTROPIS; ALPHA-MANNOSIDASE; LOCOWEED; ASTRAGALUS; UNDIFILUM; CANESCENS; PLANTS; DEFOLIATION AB Swainsonine, an indolizidine alkaloid, is an a.-mannosidase and mannosidase II inhibitor that causes lysosomal storage disease and alters glycoprotein processing. Swainsonine is found in a number of plant species worldwide, and is produced by associated endophytic fungi. Prolonged consumption of swainsonine-containing plants by livestock causes a condition characterized by weight loss, depression, altered behavior, decreased libido, infertility, and death. In contrast, Astragalus and Oxytropis that do not contain swainsonine may present a valuable food source for grazing livestock in regions where palatable forage is scarce. This study tested the hypothesis that swainsonine concentrations may be reduced by fungicide treatment or by clipping, thus reducing plant toxicity. Additionally we hypothesized that clipping plants may provide a mechanism for horizontal transmission of the endophyte. To this end, four different fungicides were applied to render the endophyte non-viable, and plant vegetative tissues were periodically clipped. Treatment of Oxytropis sericea with any of four different fungicides did not alter swainsonine concentrations in plants at any of three harvest times. Additionally, we found that individual or multiple clippings had no effect on swainsonine concentrations; plants that contained swainsonine maintained concentrations, and plants low or absent in swainsonine also remained as such at each harvest. These results suggest that there is no evidence of horizontal transmission of the endophyte among individual plants due to clipping. Published by Elsevier Ltd. C1 [Cook, Daniel; Gardner, Dale R.; Roper, Jessie M.; Pfister, James A.; Panter, Kip E.] USDA ARS, Poisonous Plant Res Lab, 1150 East 1400 North, Logan, UT 84341 USA. [Ransom, Corey V.] Utah State Univ, Dept Plants Soils & Climate, 4820 Old Main Hill, Logan, UT 84322 USA. RP Cook, D (reprint author), USDA ARS, Poisonous Plant Res Lab, 1150 East 1400 North, Logan, UT 84341 USA. EM daniel.cook@ars.usda.gov NR 38 TC 0 Z9 0 U1 7 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0041-0101 J9 TOXICON JI Toxicon PD NOV PY 2016 VL 122 BP 26 EP 30 DI 10.1016/j.toxicon.2016.09.012 PG 5 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA EB6UI UT WOS:000387520700003 PM 27644899 ER PT J AU Chen, J Cantrell, CL Oi, D Grodowitz, MJ AF Chen, Jian Cantrell, Charles L. Oi, David Grodowitz, Michael J. TI Update on the defensive chemicals of the little black ant, Monomorium minimum (Hymenoptera: Formicidae) SO TOXICON LA English DT Article DE Little black ants; Venom; Poison gland; Dufour's gland ID TRAIL-FOLLOWING PHEROMONE; LABIAL GLAND SECRETIONS; IMPORTED FIRE ANTS; DORSAL GLAND; DUFOUR GLAND; CEMBRENE-A; VOLATILE COMPONENTS; SOLENOPSIS-INVICTA; NEOCEMBRENE-A; SOFT CORAL AB Alkaloids, including 2,5-dialkylpyrrolidines and 2,5-dialkylpyrrolines, have been reported to be components in the venom of little black ants, Monomorium minimum (Buckley). Two fatty amines were recently reported as minor compounds. By analyzing the discharge collected from the stinger apparatus (milking), this study revealed the presence of an additional seven compounds in the defensive secretion of this ant species. Compounds identified were 9-decenyl-1-amine, N-methylenedecan-1-amine, N-methylenedodecan-1-amine, 2-(1-non-8-enyl)-5-(1-hex-5-enyl)-1-pyrroline, N-methyl-2-(hex-5-enyl)-5-nonanyl-1-pyrrolidine, beta-springene ((E,E)-7,11,15-trimethyl-3-methylene-1,6,10,14-hexadecatetraene) and neocembrene ((E,E,E)-1-isopropenyl-4,8,12-trimethylcyclotetradeca-3,7,11-triene). beta-springene and neocembrene were found only in the defensive secretion of queens. Analyses of the contents of isolated poison and Dufour's glands of the queen indicated that all amines and alkaloids were from the poison gland and that beta-springene and neocembrene were from the Dufour's gland. This demonstrated that the defensive secretion in M. minimum queens consists of components from both glands. This is also the first report on the natural occurrence of 9-decenyl-1-amine, N-methylenedecan-1-amine, and N-methyllenedodecan-1 -amine. Published by Elsevier Ltd. C1 [Chen, Jian; Grodowitz, Michael J.] USDA ARS, Natl Biol Control Lab, 59 Lee Rd, Stoneville, MS 38776 USA. [Cantrell, Charles L.] USDA ARS, Nat Prod Utilizat Res, University, MS 38677 USA. [Oi, David] USDA ARS, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. RP Chen, J (reprint author), USDA ARS, BCPRU, 59 Lee Rd, Stoneville, MS 38776 USA. EM jian.chen@ars.usda.gov NR 48 TC 0 Z9 0 U1 5 U2 5 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0041-0101 J9 TOXICON JI Toxicon PD NOV PY 2016 VL 122 BP 127 EP 132 DI 10.1016/j.toxicon.2016.09.009 PG 6 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA EB6UI UT WOS:000387520700017 PM 27641747 ER PT J AU Price, DJ Kacarab, M Cocker, DR Purvis-Roberts, KL Silva, PJ AF Price, Derek J. Kacarab, Mary Cocker, David R. Purvis-Roberts, Kathleen L. Silva, Philip J. TI Effects of temperature on the formation of secondary organic aerosol from amine precursors SO AEROSOL SCIENCE AND TECHNOLOGY LA English DT Article ID PARTICULATE AIR-POLLUTION; PARTICLE MASS ANALYZER; ALIPHATIC-AMINES; CHEMICAL MECHANISMS; PHOTOOXIDATION; SPECTROMETER; HYDROCARBONS; OXIDATION; CHAMBER AB Aerosol formation is directly influenced by meteorological properties such as temperature and relative humidity. This study examines the influence of temperature on the physical properties and chemical composition of the aerosol produced from radical oxidation of aliphatic amines. Aerosol formation for temperatures ranging from 10 to 40 degrees C was investigated in dual 90 m(3) indoor atmospheric chambers. Further, chemical and physical responses of aerosol formed at one temperature and then raised/cooled to another were investigated in detail. Around two to three times more aerosol formation occurred at 10 degrees C than at 40 degrees C. This has important implications for locations influenced by amine emissions during the winter months. Significant aerosol formation occurred with the oxidation of amines with nitrate radical (100-600 mg/m(3)) and consisted largely of amine nitrate salts. These reactions are important contributors to aerosol formation during the nighttime hours, when nitrate radical is the dominant oxidant and temperatures tend to be cooler. Solid/gas partitioning of amine nitrate salt aerosol was consistent with literature results. A novel, temperature dependent, mechanism describing peroxy and hydroperoxy radical reactions was observed in the trimethylamine with hydroxyl radical oxidation experiments. C1 [Price, Derek J.; Kacarab, Mary; Cocker, David R.] Univ Calif Riverside, Dept Chem & Environm Engn, 1084 Columbia Ave, Riverside, CA 92507 USA. [Price, Derek J.; Kacarab, Mary; Cocker, David R.] Univ Calif Riverside, Ctr Environm Res & Technol, Bourns Coll Engn, 1084 Columbia Ave, Riverside, CA 92507 USA. [Purvis-Roberts, Kathleen L.] Claremont Mckenna Coll, Claremont, CA 91711 USA. [Purvis-Roberts, Kathleen L.] Pitzer Coll, Claremont, CA 91711 USA. [Purvis-Roberts, Kathleen L.] Scripps Coll, WM Keck Sci Dept, Claremont, CA 91711 USA. [Silva, Philip J.] ARS, USDA, Bowling Green, KY USA. RP Cocker, DR (reprint author), Univ Calif Riverside, Dept Chem & Environm Engn, CE CERT, 1084 Columbia Ave, Riverside, CA 92507 USA. EM dcocker@engr.ucr.edu OI Price, Derek/0000-0003-3693-1475 NR 30 TC 0 Z9 0 U1 20 U2 20 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0278-6826 EI 1521-7388 J9 AEROSOL SCI TECH JI Aerosol Sci. Technol. PD NOV PY 2016 VL 50 IS 11 BP 1216 EP 1226 DI 10.1080/02786826.2016.1236182 PG 11 WC Engineering, Chemical; Engineering, Mechanical; Environmental Sciences; Meteorology & Atmospheric Sciences SC Engineering; Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA EA3BN UT WOS:000386473200006 ER PT J AU Roon, DA Wipfli, MS Wurtz, TL Blanchard, AL AF Roon, David A. Wipfli, Mark S. Wurtz, Tricia L. Blanchard, Arny L. TI Invasive European bird cherry (Prunus padus) reduces terrestrial prey subsidies to urban Alaskan salmon streams SO CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES LA English DT Article ID ENEMY RELEASE HYPOTHESIS; FOOD WEBS; RIPARIAN ZONES; HEADWATER STREAM; NITROGEN-SOURCES; AQUATIC INSECTS; TROPHIC LEVELS; RAINBOW-TROUT; LEAF-LITTER; INVERTEBRATES AB The spread of invasive species in riparian forests has the potential to affect both terrestrial and aquatic organisms linked through cross-ecosystem resource subsidies. However, this potential had not been explored in regards to terrestrial prey subsidies for stream fishes. To address this, we examined the effects of an invasive riparian tree, European bird cherry (EBC, Prunus padus), spreading along urban Alaskan salmon streams, by collecting terrestrial invertebrates present on the foliage of riparian trees, their subsidies to streams, and their consumption by juvenile coho salmon (Oncorhynchus kisutch). Riparian EBC supported four to six times less terrestrial invertebrate biomass on its foliage and contributed two to three times lower subsidies relative to native deciduous trees. This reduction in terrestrial invertebrate biomass was consistent between two watersheds over 2 years. In spite of this reduction in terrestrial prey resource input, juvenile coho salmon consumed similar levels of terrestrial invertebrates in stream reaches bordered by EBC. Although we did not see ecological effects extending to stream salmonids, reduced terrestrial prey subsidies to streams are likely to have negative consequences as EBC continues to spread. C1 [Roon, David A.] Univ Alaska, Dept Biol & Wildlife, Alaska Cooperat Fish & Wildlife Res Unit, Fairbanks, AK 99775 USA. [Wipfli, Mark S.] Univ Alaska, Inst Arctic Biol, Alaska Cooperat Fish & Wildlife Res Unit, US Geol Survey, Fairbanks, AK 99775 USA. [Wurtz, Tricia L.] US Forest Serv, USDA, Forest Hlth Program, Fairbanks, AK 99709 USA. [Blanchard, Arny L.] Univ Alaska Fairbanks, Inst Marine Sci, Fairbanks, AK 99775 USA. RP Roon, DA (reprint author), Univ Alaska, Dept Biol & Wildlife, Alaska Cooperat Fish & Wildlife Res Unit, Fairbanks, AK 99775 USA. EM david.roon@oregonstate.edu NR 65 TC 0 Z9 0 U1 5 U2 5 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0706-652X EI 1205-7533 J9 CAN J FISH AQUAT SCI JI Can. J. Fish. Aquat. Sci. PD NOV PY 2016 VL 73 IS 11 BP 1679 EP 1690 DI 10.1139/cjfas-2015-0548 PG 12 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA EA5PY UT WOS:000386674900008 ER PT J AU Piovia-Scott, J Sadro, S Knapp, RA Sickman, J Pope, KL Chandra, S AF Piovia-Scott, Jonah Sadro, Steven Knapp, Roland A. Sickman, James Pope, Karen L. Chandra, Sudeep TI Variation in reciprocal subsidies between lakes and land: perspectives from the mountains of California SO CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES LA English DT Article ID HIGH-ELEVATION LAKE; DISSOLVED ORGANIC-CARBON; AQUATIC INSECT EMERGENCE; YOSEMITE-NATIONAL-PARK; YELLOW-LEGGED FROG; SUB-ALPINE LAKE; SIERRA-NEVADA; FOOD WEBS; NONNATIVE FISH; INTRODUCED TROUT AB Lakes are connected to surrounding terrestrial habitats by reciprocal flows of energy and nutrients. We synthesize data from California's mountain lake catchments to investigate how these reciprocal subsidies change along an elevational gradient and with the introduction of a top aquatic predator. At lower elevations, well-developed terrestrial vegetation provides relatively large inputs of organic material to lakes, whereas at higher elevations, the paucity of terrestrial vegetation provides minimal organic input but allows for higher inputs of inorganic nitrogen. There are also pronounced elevational patterns in amphibians and aquatic insects, which represent important vectors for resource flows from lakes back to land. The introduction of trout can reduce this lake-to-land resource transfer, as trout consume amphibians and aquatic insects. We propose a conceptual model in which within-lake processes influence terrestrial consumers at higher elevations, while terrestrial inputs govern within-lake processes at lower elevations. This model contributes to a more general understanding of the connections between aquatic and terrestrial habitats in complex landscapes. C1 [Piovia-Scott, Jonah] Washington State Univ, Sch Biol Sci, 14204 NE Salmon Creek Ave, Vancouver, WA 98686 USA. [Sadro, Steven] Univ Calif Davis, Dept Environm Sci & Policy, One Shields Ave, Davis, CA 95616 USA. [Knapp, Roland A.] Univ Calif, Sierra Nevada Aquat Res Lab, 1016 Mt Morrison Rd, Mammoth Lakes, CA 93546 USA. [Sickman, James] Univ Calif Riverside, Dept Environm Sci, 900 Univ Ave, Riverside, CA 92521 USA. [Pope, Karen L.] US Forest Serv, Pacific Southwest Res Stn, 1700 Bayview Dr, Arcata, CA 95521 USA. [Chandra, Sudeep] Univ Nevada, Dept Biol, 1664 N Virginia St, Reno, NV 89557 USA. RP Piovia-Scott, J (reprint author), Washington State Univ, Sch Biol Sci, 14204 NE Salmon Creek Ave, Vancouver, WA 98686 USA. EM jonah.piovia-scott@wsu.edu NR 97 TC 0 Z9 0 U1 9 U2 9 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0706-652X EI 1205-7533 J9 CAN J FISH AQUAT SCI JI Can. J. Fish. Aquat. Sci. PD NOV PY 2016 VL 73 IS 11 BP 1691 EP 1701 DI 10.1139/cjfas-2015-0549 PG 11 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA EA5PY UT WOS:000386674900009 ER PT J AU Puhlick, JJ Weiskittel, AR Fernandez, IJ Fraver, S Kenefic, LS Seymour, RS Kolka, RK Rustad, LE Brissette, JC AF Puhlick, Joshua J. Weiskittel, Aaron R. Fernandez, Ivan J. Fraver, Shawn Kenefic, Laura S. Seymour, Robert S. Kolka, Randall K. Rustad, Lindsey E. Brissette, John C. TI Long-term influence of alternative forest management treatments on total ecosystem and wood product carbon storage SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE carbon stocks; forest products; forest soils; coarse fragments; site quality ID MIXED-SPECIES STANDS; CENTRAL MAINE; USA; DYNAMICS; POOLS; CONIFERS; IMPACTS; STATES; PINE AB Developing strategies for reducing atmospheric CO2 is one of the foremost challenges facing natural resource professionals today. The goal of this study was to evaluate total ecosystem and harvested wood product carbon (C) stocks among alternative forest management treatments (selection cutting, shelterwood cutting, commercial clearcutting, and no management) in mixed-species stands in central Maine, USA. These treatments were initiated in the 1950s and have been maintained since, and ecosystem C pools were measured in 2012. When compared across managed treatments, the commercial clearcut had the lowest total ecosystem C stocks by 21%, on average (P < 0.05), while the selection and shelterwood treatments had similar total ecosystem C stocks. Including the C stored in harvested wood products did not influence observed differences in C storage among treatments. Total ecosystem C stocks in the reference stand were 247.0 +/- 17.7 Mg.ha(-1) (mean +/- SD) compared with 161.7 +/- 31.3 Mg.ha(-1) in the managed stands (171.2 +/- 31.7 Mg.ha(-1) with products C). This study highlights the impacts of long-term forest management treatments on C storage and indicates that the timing of harvests and the species and sizes of trees removed influence C stored in harvested wood products. C1 [Puhlick, Joshua J.; Weiskittel, Aaron R.; Fernandez, Ivan J.; Fraver, Shawn; Seymour, Robert S.] Univ Maine, Sch Forest Resources, Orono, ME 04469 USA. [Kenefic, Laura S.] US Forest Serv, USDA, Northern Res Stn, 686 Govt Rd, Bradley, ME 04411 USA. [Kolka, Randall K.] US Forest Serv, USDA, Northern Res Stn, 1831 Hwy 169 E, Grand Rapids, MN 55744 USA. [Rustad, Lindsey E.; Brissette, John C.] US Forest Serv, USDA, Northern Res Stn, 271 Mast Rd, Durham, NH 03824 USA. RP Puhlick, JJ (reprint author), Univ Maine, Sch Forest Resources, Orono, ME 04469 USA. EM joshua.puhlick@maine.edu FU USDA Forest Service, Northern Research Station; USDA Forest Service, Northeastern States Research Cooperative; Maine Agricultural and Forest Experiment Station; Penobscot Experimental Forest Research Fund FX We thank Christopher Woodall (USDA Forest Service), Alex Finkral (The Forestland Group), and two anonymous reviewers who provided useful comments that helped us to improve this manuscript. We acknowledge Bruce Hoskins, Sue Erich, and Cheryl Spencer (University of Maine) for their advice on soil sampling and laboratory procedures. Funding for this project was provided by the USDA Forest Service, Northern Research Station and Northeastern States Research Cooperative; the Maine Agricultural and Forest Experiment Station; and the Penobscot Experimental Forest Research Fund. NR 50 TC 0 Z9 0 U1 9 U2 9 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD NOV PY 2016 VL 46 IS 11 BP 1404 EP 1412 DI 10.1139/cjfr-2016-0193 PG 9 WC Forestry SC Forestry GA EA5QD UT WOS:000386675600018 ER PT J AU Kandel, TP Elsgaard, L Andersen, MN Laerke, PE AF Kandel, Tanka P. Elsgaard, Lars Andersen, Mathias N. Laerke, Poul E. TI Influence of harvest time and frequency on light interception and biomass yield of festulolium and tall fescue cultivated on a peatland SO EUROPEAN JOURNAL OF AGRONOMY LA English DT Article DE Festulolium; Harvesting system; Perennial grasses; Radiation interception; Radiation use efficiency; Tall fescue ID REED CANARY GRASS; SOIL ORGANIC-CARBON; DRY-MATTER; AGRICULTURAL MANAGEMENT; ELEVATED-TEMPERATURE; METHANE PRODUCTION; SPRING BARLEY; WINTER-WHEAT; RAW-MATERIAL; STAY-GREEN AB In this study, we report efficiencies of light capture and biomass yield of festulolium and tall fescue cultivated on a riparian fen in Denmark under different harvesting managements. Green biomass targeted for biogas production was harvested either as two cuts (2C) or three cuts (3C) in a year. Three different timings of the first cut in the 2C systems were included as early (2C-early), middle (2C-mid) and late (2C late) cuts corresponding to pre-heading, inflorescence emergence and flowering stages, respectively. The fraction of intercepted photosynthetically active radiation (f(PAR)) was derived from the canopy reflectance measured on 61 dates throughout a year, and cumulative interception of PAR (IPAR) and radiation use efficiency (RUE) was calculated. The dynamics of f(PAR) and biomass accumulations was similar for both crops before the first cuts in all managements. Festulolium f(PAR) in 2C-early and 2C-mid managements declined faster than in 2C-late and 3C managements in the second growth period and thus growing period IPAR of 2C-early and 2C-mid declined by 8% as compared to 3C management where IPAR was 925 MJ m(-2). Annual festulolium dry matter (DM) yield in 2C-early and 2C-mid managements (average 14.1 Mg DM ha(-1)) decreased by 22% compared to 3C management (18.2 Mg DM ha(-1)). The highest and the lowest RUE of festulolium were observed in 3C and 2C-mid managements as 1.97 and 1.59 g MJ(-1), respectively. For tall fescue f(PAR) declined rather slowly in the second growing period in all 2C managements, which contributed to similar IPAR (908-919 MJ m(-2)), total biomass yield (16.4-18.8 Mg DM ha(-1) yr(-1)) and RUE (1.80-2.07 g MJ(-1)) for all managements. Whereas both crops were highly productive under both 3C management and 2C management with first harvest after flowering (i.e., 2C-late), the 2C-late strategy is recommended as the least intensive of the two management systems. (C) 2016 Elsevier B.V. All rights reserved. C1 [Kandel, Tanka P.; Elsgaard, Lars; Andersen, Mathias N.; Laerke, Poul E.] Aarhus Univ, Dept Agroecol, POB 50, DK-8830 Tjele, Denmark. RP Kandel, TP (reprint author), USDA ARS, Forage & Livestock Prod Res Unit, Grazinglands Res Lab, 7207 W Cheyenne St, El Reno, OK 73036 USA. EM tankakandel@gmail.com RI Andersen, Mathias Neumann/A-7658-2008; Kandel, Tanka/M-5764-2016; OI Andersen, Mathias Neumann/0000-0003-3845-4465; Kandel, Tanka/0000-0003-2543-9602; Laerke, Poul Erik/0000-0002-2224-3893 FU DCA - National Centre for Food and Agriculture; Innovation Fund Denmark under the EU FACCE-ERA-NET+ on Climate Smart Agriculture as a part of the CAOS project FX The study was supported by funding from the DCA - National Centre for Food and Agriculture (http://dca.au.dk/en/) as a part of the BioX project and by the Innovation Fund Denmark under the EU FACCE-ERA-NET+ on Climate Smart Agriculture as a part of the CAOS project http://www.caos-project.eu/. The authors like to express their gratitude for assistance of Jens B. Kjeldsen and the team of technicians from AU-Foulumgard in crop managements and harvesting. We also like to extend our gratitude to internship students Suman Thapa and Ben McCarthy for their assistance in grass harvesting. NR 65 TC 1 Z9 1 U1 9 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1161-0301 EI 1873-7331 J9 EUR J AGRON JI Eur. J. Agron. PD NOV PY 2016 VL 81 BP 150 EP 160 DI 10.1016/j.eja.2016.09.010 PG 11 WC Agronomy SC Agriculture GA EA9RN UT WOS:000386982500016 ER PT J AU Kim, GH Fratamico, P Breidt, F Oh, DH AF Kim, G-H. Fratamico, P. Breidt, F. Oh, D-H. TI Survival and expression of acid resistance genes in Shiga toxin-producing Escherichia coli acid adapted in pineapple juice and exposed to synthetic gastric fluid SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE acid resistance; gene expression; pineapple juice; real-time quantitative-PCR; Shiga toxin-producing Escherichia coli; synthetic gastric fluid ID REAL-TIME PCR; MEMBRANE-LIPID COMPOSITION; VEROTOXIN SECRETION; STRESS-RESPONSE; ORGANIC-ACIDS; APPLE JUICE; ACETIC-ACID; REDUCED PH; O157-H7; O157H7 AB AimsThe aim of this research was to determine the ability of non-O157 Shiga toxin-producing Escherichia coli (STEC) serogroups to survive with exposure to synthetic gastric fluid (SGF) after adaptation to pineapple juice (PJ) at room and refrigerated temperatures compared to E.coli O157:H7 and to examine the relative transcriptional expression of acid resistance (AR) genes, rpoS, gadA and adiA. Methods and ResultsResistant and sensitive strains belonging to five different STEC serogroups (O26, O103, O104, O111 and O157; n=10) were used in this study. All strains were adapted in PJ (pH 38) stored at 4 and 20 degrees C for 24h, and then the relative transcription levels of genes in all strains were quantified using a real-time quantitative-PCR assay. After adaptation in PJ, the STEC strains were exposed to SGF (pH 15 and 20) at 37 degrees C for 2h. Generally, the STEC adapted in PJ at 4 degrees C displayed enhanced survival compared to acid adaptation in PJ at 20 degrees C and nonadapted controls with exposure to SGF (P<005). Moreover, resistant strains exhibited higher survival rates compared to sensitive strains (P<005). Overall, adaptation at 4 degrees C resulted in significantly (P<005) enhanced gene expression levels in PJ, and transcript levels of gadA were higher than those of the rpoS and adiA genes. ConclusionsThe up-regulation of AR genes due to adaptation in PJ at low temperature may increase STEC survival in acidic environments such as the gastrointestinal tract. Some non-O157 STEC strains, including serotypes O103:H2 and O111:H8, showed relatively high AR levels similar to those of STEC O157:H7. Significance and Impact of the StudyInduction of AR genes in acidic fruit juice, and potentially in other acidic foods may increase the risk of foodborne illness by non-O157 STEC serogroups. C1 [Kim, G-H.; Oh, D-H.] Kangwon Natl Univ, Dept Food Sci & Biotechnol, Chunchon, Gangwon, South Korea. [Kim, G-H.; Fratamico, P.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA USA. [Breidt, F.] North Carolina State Univ, USDA ARS, Food Sci Res Unit, Dept Food Bioproc & Nutr Sci, Raleigh, NC USA. RP Oh, DH (reprint author), Kangwon Natl Univ, Dept Food Sci & Biotechnol, 1 Kangwondaehak Gil, Chuncheon Si 200701, Gangwon Do, South Korea. EM deoghwa@kanagwon.ac.kr FU Kangwon National University [120131286] FX This study was supported by 2013 Research Grants from Kangwon National University (No. 120131286). NR 37 TC 0 Z9 0 U1 6 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 EI 1365-2672 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD NOV PY 2016 VL 121 IS 5 BP 1416 EP 1426 DI 10.1111/jam.13223 PG 11 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA EA6RY UT WOS:000386757800020 PM 27377411 ER PT J AU Zhang, Y Long, RJ Anderson, RC Humec, ME Coverdale, JA Latham, EA Nisbet, DJ AF Zhang, Ying Long, Ruijun Anderson, Robin C. Humec, Michael E. Coverdale, Josie A. Latham, Elizabeth A. Nisbet, David J. TI Characterization of Nitrate-Reducing and Amino Acid-Using Bacteria Prominent in Nitrotoxin-Enriched Equine Cecal Populations SO JOURNAL OF EQUINE VETERINARY SCIENCE LA English DT Article DE Equine; Nitrotoxin metabolism; DGGE; Sporanaerobacter acetigens ID AMERICAN ASTRAGALUS FABACEAE; GEN. NOV.; TOXINS; NITROCOMPOUNDS; NITROPROPANOL; METABOLISM; DIVERSITY; METHANE; CATTLE; RUMEN AB Horses can be at risk for nitropoisoning by consuming plants containing 3-nitro-1-propionic acid or 3-nitro-1-propanol and to a lesser extent by plants containing nitrate. Populations of equine cecal microbes enriched for enhanced rates of 3-nitro-1-propionic acid (NPA) or nitrate metabolism were cultured for NPA- or nitrate-metabolizing bacteria on basal enrichment medium or tryptose soy agar supplemented with either 5-mM NPA or nitrate and under H-2:CO2 (20:80) as the energy source. After 72 hours, separated colonies picked from plates, or roll tubes were cultured in fresh broth medium for 72 hours and then identified by 16S rRNA gene sequencing. Isolates from the NPA-enriched populations were identified as Streptococcus lutetiensis (five strains), Escherichia coli (two strains), and Sporanaerobacter acetigens (one strain). Strains isolated from nitrate-enriched populations were identified as Escherichia coli (one strain) and Wolinella succinogenes (three strains). None of these strains degraded NPA. Enriched populations of equine cecal microbes, the isolated pure strains and the type strain of Denitrobacterium detoxificans, a competent NPA-metabolizing microbe, were examined using denaturing gradient gel electrophoresis (DGGE). The DGGE analysis indicated that none of the strains in the enriched population of equine cecal microbes were similar to D. detoxificans. However, we report for the first time the isolation of the anaerobic amino acid-using Sporanaerobacter acetigenes from the equine cecum. Published by Elsevier Inc. C1 [Zhang, Ying] Lanzhou Univ, Sch Publ Hlth, Lanzhou, Peoples R China. [Long, Ruijun] Lanzhou Univ, Sch Life Sci, Lanzhou, Peoples R China. [Anderson, Robin C.; Humec, Michael E.; Nisbet, David J.] ARS, USDA, College Stn, TX USA. [Coverdale, Josie A.; Latham, Elizabeth A.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA. RP Anderson, RC (reprint author), ARS, USDA, Southern Plains Agr Res Ctr, Food & Feed Safety Res Unit, 2881 F&B Rd, College Stn, TX 77845 USA. EM robin.anderson@ars.usda.gov FU National Natural Science Foundation of China [31501976]; Fundamental Research Funds for the Central Universities [lzujbky-2015-183, lzujbky-2015-261] FX This research was supported in part by the National Natural Science Foundation of China (31501976) and the Fundamental Research Funds for the Central Universities (lzujbky-2015-183; lzujbky-2015-261). The technical assistance of Jackie Kotzur and Charles Hernandez is appreciated. NR 23 TC 0 Z9 0 U1 6 U2 6 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0737-0806 EI 1542-7412 J9 J EQUINE VET SCI JI J. Equine Vet. Sci. PD NOV PY 2016 VL 46 BP 47 EP 53 DI 10.1016/j.jevs.2016.04.007 PG 7 WC Veterinary Sciences SC Veterinary Sciences GA EA7QI UT WOS:000386826200008 ER PT J AU Lacombe, A Niemira, BA Sites, J Boyd, G Gurtler, JB Tyrell, B Fleck, M AF Lacombe, Alison Niemira, Brendan A. Sites, Joseph Boyd, Glenn Gurtler, Joshua B. Tyrell, Breanna Fleck, Melissa TI Reduction of Bacterial Pathogens and Potential Surrogates on the Surface of Almonds Using High-Intensity 405-Nanometer Light SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Escherichia coli O157:H7; Food safety; High-intensity light; Light-emitting diodes; Salmonella; Surrogate ID ESCHERICHIA-COLI O157/H7; NM LIGHT; SALMONELLA-ENTERITIDIS; LISTERIA-MONOCYTOGENES; INOCULATED ALMONDS; MICROORGANISMS; INACTIVATION; SURVIVAL; QUALITY AB The disinfecting properties of high-intensity monochromatic blue light (MBL) were investigated against Escherichia coli O157:H7, Salmonella, and nonpathogenic bacteria inoculated onto the surface of almonds. MBL was generated from an array of narrow-band 405-nm light-emitting diodes. Almonds were inoculated with higher or lower levels (8 or 5 CFU/g) of pathogenic E. coli O157:H7 and Salmonella, as well as nonpathogenic E. coli K-12 and an avirulent strain of Salmonella Typhimurium, for evaluation as potential surrogates for their respective pathogens. Inoculated almonds were treated with MBL for 0, 1, 2, 4, 6, 8, and 10 min at a working distance of 7 cm. Simultaneous to treatment, cooling air was directed onto the almonds at a rate of 4 ft(3)/min (1.89 x 10(-3) m(3)/s), sourced through a container of dry ice. An infrared camera was used to monitor the temperature readings after each run. For E. coli K-12, reductions of up to 1.85 or 1.63 log CFU/g were seen for higher and lower inoculum levels, respectively; reductions up to 2.44 and 1.44 log CFU/g were seen for E. coli O157:H7 (higher and lower inoculation levels, respectively). Attenuated Salmonella was reduced by up to 0.54 and 0.97 log CFU/g, whereas pathogenic Salmonella was reduced by up to 0.70 and 0.55 log CFU/g (higher and lower inoculation levels, respectively). Inoculation level did not significantly impact minimum effective treatment times, which ranged from 1 to 4 min. Temperatures remained below ambient throughout treatment, indicating that MBL is a nonthermal antimicrobial process. The nonpathogenic strains of E. coli and Salmonella each responded to MBL in a comparable manner to their pathogenic counterparts. These results suggest that these nonpathogenic strains may be useful in experiments with MBL in which a surrogate is required, and that MBL warrants further investigation as a potential antimicrobial treatment for low-moisture foods. C1 [Lacombe, Alison] Natl Coll Nat Med, 014 Southeast Porter St, Portland, OR 97201 USA. [Niemira, Brendan A.; Sites, Joseph; Boyd, Glenn; Gurtler, Joshua B.; Tyrell, Breanna; Fleck, Melissa] ARS, Food Safety & Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, 600 Mermaid Lane, Wyndmoor, PA 19038 USA. RP Niemira, BA (reprint author), ARS, Food Safety & Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, 600 Mermaid Lane, Wyndmoor, PA 19038 USA. EM Brendan.Niemira@ars.usda.gov FU U.S. Department of Agriculture, National Institute of Food and Agriculture, NIPA award [2015-69003-23410] FX The authors acknowledge U.S. Department of Agriculture, National Institute of Food and Agriculture, NIPA award no. 2015-69003-23410. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. NR 28 TC 0 Z9 0 U1 4 U2 4 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD NOV PY 2016 VL 79 IS 11 BP 1840 EP 1845 DI 10.4315/0362-028X.JFP-15-418 PG 6 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA EB1FG UT WOS:000387094400003 PM 28221904 ER PT J AU Wang, R Luedtke, BE Bosilevac, JM Schmidt, JW Kalchayanand, N Arthur, TM AF Wang, Rong Luedtke, Brandon E. Bosilevac, Joseph M. Schmidt, John W. Kalchayanand, Norasak Arthur, Terrance M. TI Escherichia coli O157:H7 Strains Isolated from High-Event Period Beef Contamination Have Strong Biofilm-Forming Ability and Low Sanitizer Susceptibility, Which Are Associated with High pO157 Plasmid Copy Number SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Biofilm; Escherichia coli O157:H7; High-event period; pO157 plasmid; Sanitizer ID SALMONELLA-ENTERICA; RESISTANCE; CATTLE; DIVERSITY; O157-H7; SAKAI AB In the meat industry, a high-event period (HEP) is defined as a time period when beef processing establishments experience an increased occurrence of product contamination by Escherichia coli O157:H7. Our previous studies suggested that bacterial biofilm formation and sanitizer resistance might contribute to HEPs. We conducted the present study to further characterize E. coli O157:H7 strains isolated during HEPs for their potential to cause contamination and to investigate the genetic basis for their strong biofilm-forming ability and high sanitizer resistance. Our results show that, compared with the E. coli O157:H7 diversity control panel strains, the HEP strains had a significantly higher biofilm-forming ability on contact surfaces and a lower susceptibility to common sanitizers. No difference in the presence of disinfectant-resistant genes or the prevalence of antibiotic resistance was observed between the HEP and control strains. However, the HEP strains retained significantly higher copy numbers of the pO157 plasmid. A positive correlation was observed among a strain's high plasmid copy number, strong biofilmforming ability, low sanitizer susceptibility, and high survival and recovery capability after sanitization, suggesting that these specific phenotypes could be either directly correlated to gene expression on the pO157 plasmid or indirectly regulated via chromosomal gene expression influenced by the presence of the plasmid. Our data highlight the potential risk of biofilm formation and sanitizer resistance in HEP contamination by E. coli O157:H7, and our results call for increased attention to proper and effective sanitization practices in meat processing facilities. C1 [Wang, Rong; Luedtke, Brandon E.; Bosilevac, Joseph M.; Schmidt, John W.; Kalchayanand, Norasak; Arthur, Terrance M.] ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, Clay Ctr, NE 68933 USA. [Luedtke, Brandon E.] Univ Nebraska Kearney, Dept Biol, 2401 11th Ave, Kearney, NE 68849 USA. RP Wang, R (reprint author), ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, Clay Ctr, NE 68933 USA. EM rong.wang@ars.usda.gov FU Nebraska Beef Council [58-5438-4-014] FX The authors thank Dr. Shaohua Zhao (FDA, Laurel, MD) for kindly providing E. coli strains as positive controls for sanitizer-resistant gene detection and Dr. Likou Zou (Sichuan Agricultural University, Sichuan, People's Republic of China) for kindly providing primer sequences and protocols for the PCR amplification experiments. The authors also thank Alberto O. Alvarado and Bruce Jasch for technical support, and Jody Gallagher for secretarial assistance. This study was supported by Nebraska Beef Council Grant No. 58-5438-4-014. Names are necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval of the product to the exclusion of others that may also be suitable. NR 20 TC 0 Z9 0 U1 1 U2 1 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD NOV PY 2016 VL 79 IS 11 BP 1875 EP 1883 DI 10.4315/0362-028X.JFP-16-113 PG 9 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA EB1FG UT WOS:000387094400008 PM 28221917 ER PT J AU Tyson, GH Tate, HP Abbott, J Tran, TT Kabera, C Crarey, E Young, SN McDermott, PF Sprague, G Campbell, M Adeyemo, O Browne-Silva, J Myers, M Thitaram, S Zhao, SH AF Tyson, Gregory H. Tate, Heather P. Abbott, Jason Thu-Thuy Tran Kabera, Claudine Crarey, Emily Young, Shenia McDermott, Patrick F. Sprague, Grisselle Campbell, Mark Adeyemo, Oyewole Browne-Silva, Johnette Myers, Michael Thitaram, Sutawee Zhao, Shaohua TI Molecular Subtyping and Source Attribution of Campylobacter Isolated from Food Animals SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Campylobacter; Food animals; Pulsed-field gel electrophoresis; Source attribution ID FIELD GEL-ELECTROPHORESIS; UNITED-STATES; ANTIMICROBIAL RESISTANCE; JEJUNI CLONE; RETAIL MEATS; OUTBREAK; PATHOGENS; COLI; INFECTIONS; PREVALENCE AB Campylobacter spp. commonly cause gastrointestinal illness in humans. Poultry meats have long been considered the predominant source of these infections, but few in-depth Campylobacter source attribution studies have been completed. We analyzed more than 1,300 Campylobacter isolates recovered from a number of animal and food sources, including dairy and beef cattle, pigs, poultry, and retail poultry meat, and compared them with Campylobacter isolates recovered from human clinical samples. Each isolate was subtyped using pulsed-field gel electrophoresis (PFGE) with SmaI and queried against the Centers for Disease Control and Prevention PulseNet database to identify human isolates with indistinguishable patterns. Half (49.5%) of the PFGE patterns from poultry animal and retail meat isolates were indistinguishable from patterns of at least one human isolate. Among the isolates from beef and dairy cows, 56.6 and 65.0%, respectively, of their PFGE patterns were indistinguishable from those of human isolates. Only a small portion of the PFGE patterns of Campylobacter isolated from pigs (9.5%) were found to have PFGE patterns in common with human isolates. These data imply that cattle may be larger contributors to Campylobacter infections than previously recognized and help further our understanding of potential sources of human campylobacteriosis. C1 [Tyson, Gregory H.; Tate, Heather P.; Abbott, Jason; Thu-Thuy Tran; Kabera, Claudine; Crarey, Emily; Young, Shenia; McDermott, Patrick F.; Zhao, Shaohua] US FDA, Ctr Vet Med, 8401 Muirkirk Rd, Laurel, MD 20708 USA. [Sprague, Grisselle; Campbell, Mark; Adeyemo, Oyewole; Browne-Silva, Johnette; Myers, Michael; Thitaram, Sutawee] US Food Safety & Inspect Serv, USDA, 950 Coll Stn Rd, Athens, GA 30601 USA. RP Tyson, GH (reprint author), US FDA, Ctr Vet Med, 8401 Muirkirk Rd, Laurel, MD 20708 USA. EM Gregory.Tyson@fda.hhs.gov NR 32 TC 0 Z9 0 U1 6 U2 6 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD NOV PY 2016 VL 79 IS 11 BP 1891 EP 1897 DI 10.4315/0362-028X.JFP-16-195 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA EB1FG UT WOS:000387094400010 PM 28221911 ER PT J AU Berrang, ME Ladely, SR Meinersmann, RJ Line, JE Oakley, BB Cox, NA AF Berrang, M. E. Ladely, S. R. Meinersmann, R. J. Line, J. E. Oakley, B. B. Cox, N. A. TI Variation in Campylobacter Multilocus Sequence Typing Subtypes from Chickens as Detected on Three Plating Media SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Broiler; Campylobacter; Chicken; Multilocus sequence typing; Plating media ID CROSS-CONTAMINATION; BROILER FLOCKS; CARCASSES; JEJUNI; SPP.; SLAUGHTERHOUSE; DIVERSITY; DYNAMICS; FILTERS; AGAR AB The objective of this study was to compare subtypes of Campylobacter jejuni and Campylobacter coli detected on three selective Campylobacter plating media to determine whether each medium selected for different subtypes. Fifty ceca and 50 carcasses (representing 50 flocks) were collected from the evisceration line in a commercial broiler processing plant. Campylobacter was cultured and isolated from cecal contents and carcass rinses on Campy-Cefex, Campy Line, and RF Campylobacter jejuni coli agars. When a positive result was obtained with all three media, one colony of the most prevalent morphology on each medium was selected for further analysis by full genome sequencing and multilocus sequence typing. Sequence types were assigned according to PubMLST. A total of 49 samples were positive for Campylobacter on all three media. Forty samples contained only C. jejuni, three had only C. coli, and both species were detected in six samples. From 71% of samples, Campylobacter isolates of the same sequence type were recovered on all three media. From 81.6% of samples, isolates were all from the same clonal complex. From significantly fewer samples (26%, P < 0.01), one medium recovered an isolate with a sequence type different from the type recovered on the other two media. When multiple sequence types were detected, six times the medium with the odd sequence type was Campy-Cefex, four times it was Campy-Line, and six times it was RF Campylobacter jejuni/coli. From one sample, three sequence types were detected. In most cases, all three plating media allowed detection of the same type of Campylobacter from complex naturally contaminated chicken samples. C1 [Berrang, M. E.; Meinersmann, R. J.; Line, J. E.; Cox, N. A.] ARS, USDA, US Natl Poultry Res Ctr, Athens, GA 30505 USA. [Ladely, S. R.] Food Safety Inspect Serv, USDA, Athens, GA 30605 USA. [Oakley, B. B.] Western Univ Hlth Sci, Coll Vet Med, Pomona, CA 91766 USA. RP Berrang, ME (reprint author), ARS, USDA, US Natl Poultry Res Ctr, Athens, GA 30505 USA. EM mark.berrang@ars.usda.gov NR 19 TC 0 Z9 0 U1 1 U2 1 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD NOV PY 2016 VL 79 IS 11 BP 1986 EP 1989 DI 10.4315/0362-028X.JFP-16-188 PG 4 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA EB1FG UT WOS:000387094400024 PM 28221919 ER PT J AU Poudyal, NC Bowker, JM Moore, RL AF Poudyal, Neelam C. Bowker, J. M. Moore, Rebecca L. TI Understanding Public Knowledge and Attitudes toward Controlling Hemlock Woolly Adelgid on Public Forests SO JOURNAL OF FORESTRY LA English DT Article DE biological control; forest pest; hemlock; insecticide; invasive species; public opinion ID NATURAL DISTURBANCE; MANAGEMENT; PERCEPTIONS; IMPACTS; BEETLE AB Hemlock woolly adelgid (HWA) (Adelges tsugae), an exotic forest pest, has infested millions of acres of eastern hemlock (Tsuga canadensis) forest from Georgia to Maine. Hemlock mortality has been linked to a loss in esthetics, declines in trout habitat, and an increase in safety hazards in public forests. Available options to control HWA vary in terms of cost and environmental impact. As infestations occur often on public lands, which are popular recreation destinations, understanding public knowledge of and attitudes toward HWA control options will be important to any successful control program. Household survey results indicate public support for combating HWA, but public awareness of control options and of HWA in general is low. Survey respondents revealed concerns related to increased risk of wildfire, safety, and reduced quality of recreation but were less concerned with property value reduction or loss of trees on private land. Spraying oil and soap on branches and injecting insecticides into tree trunks were preferred to chemical soil treatments or releasing nonnative predatory beetles. Both site-and user group-specific differences in acceptability were observed for alternative control options, suggesting a need for careful selection of control options, depending on site characteristics and user populations. C1 [Poudyal, Neelam C.] Univ Tennessee, Dept Forestry Wildlife & Fisheries, Knoxville, TN 37996 USA. [Bowker, J. M.] US Forest Serv, USDA, Athens, GA USA. [Moore, Rebecca L.] Bur Land Management, Ft Collins, CO USA. RP Poudyal, NC (reprint author), Univ Tennessee, Dept Forestry Wildlife & Fisheries, Knoxville, TN 37996 USA. EM npoudyal@utk.edu; mbowker@fs.fed.us; rmoore@blm.gov FU USDA Forest Service FX We acknowledge the funding support provided by the USDA Forest Service to complete this study and the constructive comments provided by editors and three anonymous reviewers of this journal to improve the article. NR 39 TC 0 Z9 0 U1 10 U2 10 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD NOV PY 2016 VL 114 IS 6 BP 619 EP 628 DI 10.5849/jof.15-015 PG 10 WC Forestry SC Forestry GA EA9WB UT WOS:000386994300003 ER PT J AU Butler, BJ Hewes, JH Dickinson, BJ Andrejczyk, K Butler, SM Markowski-Lindsay, M AF Butler, Brett J. Hewes, Jaketon H. Dickinson, Brenton J. Andrejczyk, Kyle Butler, Sarah M. Markowski-Lindsay, Marla TI Family Forest Ownerships of the United States, 2013: Findings from the USDA Forest Service's National Woodland Owner Survey SO JOURNAL OF FORESTRY LA English DT Article DE private forest owners; nonindustrial private forest owners; attitudes; behaviors; demographics; management; trends ID BEHAVIOR; MANAGEMENT; LANDOWNERS AB There are an estimated 10.7 million family forest ownerships across the United States who collectively control 36% or 290 million acres of the nation's forestland. The US Department of Agriculture Forest Service National Woodland Owner Survey (NWOS) provides information on the characteristics, attitudes, and behaviors of these ownerships. Between 2011 and 2013, 8,576 randomly selected family forest ownerships with at least 10 acres of forestland participated in the NWOS. Results show: amenity values are the dominant reasons for owning; owners tend to be active on their land, but most are not engaged in traditional forestry programs; and owners are relatively old. Although the general ownership patterns and reasons for owning are the same between the 2002-2006 and current iterations of the NWOS, participation in some management activities changed (some increased and some decreased) and the percentage of female primary decisionmakers increased. C1 [Butler, Brett J.] US Forest Serv, USDA, No Res Stn, Amherst, MA 01003 USA. [Hewes, Jaketon H.; Dickinson, Brenton J.; Andrejczyk, Kyle; Butler, Sarah M.; Markowski-Lindsay, Marla] Univ Massachusetts Amherst, Amherst, MA USA. RP Butler, BJ (reprint author), US Forest Serv, USDA, No Res Stn, Amherst, MA 01003 USA. EM bbutler01@fs.fed.us; jhewes@eco.umass.edu; sbutler@eco.umass.edu; marla@eco.umass.edu FU Research and Development and State and Private Forestry deputy areas of the USDA Forest Service FX The NWOS would not be possible without the cooperation of thousands of family forest owners from across the United States. We are also thankful for the efforts of the FIA field crew and other FIA staff that make the NWOS possible and the reviewers who helped to improve the quality of this article. Funding for the NWOS comes from the Research and Development and State and Private Forestry deputy areas of the USDA Forest Service. The NWOS is implemented by the Family Forest Research Center (www.fitmilyforestresearchcenter.org), a joint venture between the USDA Forest Service and the University of Massachusetts Amherst. NR 26 TC 0 Z9 0 U1 2 U2 2 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD NOV PY 2016 VL 114 IS 6 BP 638 EP 647 DI 10.5849/jof.15-099 PG 10 WC Forestry SC Forestry GA EA9WB UT WOS:000386994300005 ER PT J AU Maes, PW Rodrigues, PAP Oliver, R Mott, BM Anderson, KE AF Maes, Patrick W. Rodrigues, Pedro A. P. Oliver, Randy Mott, Brendon M. Anderson, Kirk E. TI Diet-related gut bacterial dysbiosis correlates with impaired development, increased mortality and Nosema disease in the honeybee (Apis mellifera) SO MOLECULAR ECOLOGY LA English DT Article DE Diet; Frischella perrara; gut microbiota; hypopharyngeal gland; Ileum; mortality; Nosema spp.; nurse development; rectum; Snodgrassella alvi; stored pollen ID PARASACCHARIBACTER-APIUM; BUMBLE BEES; GEN. NOV.; MICROBIOTA; POLLEN; TRANSMISSION; COMMUNITIES; EXPRESSION; DIVERSITY; INFECTION AB Dysbiosis, defined as unhealthy shifts in bacterial community composition, can lower the colonization resistance of the gut to intrinsic pathogens. Here, we determined the effect of diet age and type on the health and bacterial community composition of the honeybee (Apis mellifera). We fed newly emerged bees fresh or aged diets, and then recorded host development and bacterial community composition from four distinct regions of the hosts' digestive tract. Feeding fresh pollen or fresh substitute, we found no difference in host mortality, diet consumption, development or microbial community composition. In contrast, bees fed aged diets suffered impaired development, increased mortality and developed a significantly dysbiotic microbiome. The consumption of aged diets resulted in a significant reduction in the core ileum bacterium Snod-grassella alvi and a corresponding increase in intrinsic pathogen Frischella perrara. Moreover, the relative abundance of S. alvi in the ileum was positively correlated with host survival and development. The inverse was true for both F. perrara and Parasacharibacter apium. Collectively, our findings suggest that the early establishment of S. alvi is associated with healthy nurse development and potentially excludes F. perrara and P. apium from the ileum. Although at low abundance, establishment of the common midgut pathogen Nosema spp. was significantly associated with ileum dysbiosis and associated host deficiencies. Moreover, dysbiosis in the ileum was reflected in the rectum, mouthparts and hypopharyngeal glands, suggesting a systemic host effect. Our findings demonstrate that typically occurring alterations in diet quality play a significant role in colony health and the establishment of a dysbiotic gut microbiome. C1 [Maes, Patrick W.; Rodrigues, Pedro A. P.; Anderson, Kirk E.] Univ Arizona, Grad Interdisciplinary Program Entomol & Insect S, POB 210036, Tucson, AZ 85721 USA. [Maes, Patrick W.; Rodrigues, Pedro A. P.; Anderson, Kirk E.] Univ Arizona, Dept Entomol, POB 210036, Tucson, AZ 85721 USA. [Oliver, Randy] ScientificBeekeeping Com, 14744 Meadow Dr, Grass Valley, CA 95945 USA. [Mott, Brendon M.; Anderson, Kirk E.] ARS, USDA, Carl Hayden Bee Res Ctr, 2000 East Allen Rd, Tucson, AZ 85719 USA. RP Maes, PW (reprint author), Univ Arizona, Grad Interdisciplinary Program Entomol & Insect S, POB 210036, Tucson, AZ 85721 USA.; Maes, PW (reprint author), Univ Arizona, Dept Entomol, POB 210036, Tucson, AZ 85721 USA. EM pmaes@email.arizona.edu OI Rodrigues, Pedro/0000-0002-3280-7576 NR 63 TC 1 Z9 1 U1 28 U2 28 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1083 EI 1365-294X J9 MOL ECOL JI Mol. Ecol. PD NOV PY 2016 VL 25 IS 21 BP 5439 EP 5450 DI 10.1111/mec.13862 PG 12 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA EA1LH UT WOS:000386353200013 PM 27717118 ER PT J AU Nacci, D Proestou, D Champlin, D Martinson, J Waits, ER AF Nacci, Diane Proestou, Dina Champlin, Denise Martinson, John Waits, Eric R. TI Genetic basis for rapidly evolved tolerance in the wild: adaptation to toxic pollutants by an estuarine fish species SO MOLECULAR ECOLOGY LA English DT Article DE adaptation; contemporary evolution; dioxin; evolved tolerance; quantitative trait locus ID ARYL-HYDROCARBON RECEPTOR; KILLIFISH FUNDULUS-HETEROCLITUS; POLYCYCLIC AROMATIC-HYDROCARBONS; MARINE SUPERFUND SITE; ATLANTIC KILLIFISH; DEVELOPMENTAL TOXICITY; POPULATION GENOMICS; NATURAL-POPULATIONS; ZEBRAFISH; EVOLUTION AB Atlantic killifish (Fundulus heteroclitus) residing in some urban and industrialized estuaries of the US eastern seaboard demonstrate recently evolved and extreme tolerance to toxic aryl hydrocarbon pollutants, characterized as dioxin-like compounds (DLCs). Here, we provide an unusually comprehensive accounting (69%) through quantitative trait locus (QTL) analysis of the genetic basis for DLC tolerance in killifish inhabiting an urban estuary contaminated with PCB congeners, the most toxic of which are DLCs. Consistent with mechanistic knowledge of DLC toxicity in fish and other vertebrates, the aryl hydrocarbon receptor (ahr2) region accounts for 17% of trait variation; however, QTL on independent linkage groups and their interactions have even greater explanatory power (44%). QTL interpreted within the context of recently available Fundulus genomic resources and shared synteny among fish species suggest adaptation via interacting components of a complex stress response network. Some QTL were also enriched in other killifish populations characterized as DLC-tolerant and residing in distant urban estuaries contaminated with unique mixtures of pollutants. Together, our results suggest that DLC tolerance in killifish represents an emerging example of parallel contemporary evolution that has been driven by intense human-mediated selection on natural populations. C1 [Nacci, Diane; Proestou, Dina; Champlin, Denise] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Atlantic Ecol Div, 27 Tarzwell Dr, Narragansett, RI 02882 USA. [Martinson, John; Waits, Eric R.] US EPA, Off Res & Dev, Natl Exposure Res Lab, Ecol Exposure Res Div, 26 W Martin Luther King Dr, Cincinnati, OH 45268 USA. [Proestou, Dina] Univ Rhode Isl, ARS, USDA,Shellfish Genet, Northeast Area,Natl Cold Water Marine Aquaculture, 469 CBLS,120 Flagg Rd, Kingston, RI 02881 USA. RP Nacci, D (reprint author), US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Atlantic Ecol Div, 27 Tarzwell Dr, Narragansett, RI 02882 USA. EM Nacci.diane@epa.gov FU National Science Foundation [DEB-1120512] FX This is tracking # ORD-011310 of the US Environmental Protection Agency, Office of Research and Development. This manuscript has been reviewed and approved for publication by the US EPA. An early version of the manuscript benefitted greatly by reviews by Dr. Bryan Clark, Dr. Mark Hahn, Dr. Jeffrey Markert, Dr. Isaac Wirgin, Dr. Caird E. Rexroad III and Mr. Joseph LiVolsi. Technical assistance was provided by Ashley Bertrand and Ian Kirby; graphic design was provided by Patricia DeCastro, SRA, Narragansett, RI. Approval does not signify that the contents necessarily reflect the views and policies of the US EPA. Mention of trade names, products or services does not convey, and should not be interpreted as conveying official US EPA approval, endorsement or recommendation. Data interpretation was aided by reference to a preliminary draft of the F. heteroclitus genome sequence which was supported by funding from the National Science Foundation (DEB-1120512). NR 63 TC 1 Z9 1 U1 17 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1083 EI 1365-294X J9 MOL ECOL JI Mol. Ecol. PD NOV PY 2016 VL 25 IS 21 BP 5467 EP 5482 DI 10.1111/mec.13848 PG 16 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA EA1LH UT WOS:000386353200015 PM 27662639 ER PT J AU Lin, JY Mazarei, M Zhao, N Hatcher, CN Wuddineh, WA Rudis, M Tschaplinski, TJ Pantalone, VR Arelli, PR Hewezi, T Chen, F Stewart, CN AF Lin, Jingyu Mazarei, Mitra Zhao, Nan Hatcher, Catherine N. Wuddineh, Wegi A. Rudis, Mary Tschaplinski, Timothy J. Pantalone, Vincent R. Arelli, Prakash R. Hewezi, Tarek Chen, Feng Stewart, Charles Neal, Jr. TI Transgenic soybean overexpressing GmSAMT1 exhibits resistance to multiple-HG types of soybean cyst nematode Heterodera glycines SO PLANT BIOTECHNOLOGY JOURNAL LA English DT Article DE salicylic acid methyltransferase; transgenic soybean; soybean cyst nematode; gene expression; metabolite; female index; yield ID SYSTEMIC ACQUIRED-RESISTANCE; SALICYLIC-ACID; ARABIDOPSIS-THALIANA; CHORISMATE MUTASE; METHYL SALICYLATE; GENE-EXPRESSION; PARASITISM; DEFENSE; ROOTS; BIOSYNTHESIS AB Soybean (Glycine max (L.) Merr.) salicylic acid methyl transferase (GmSAMT1) catalyses the conversion of salicylic acid to methyl salicylate. Prior results showed that when GmSAMT1 was overexpressed in transgenic soybean hairy roots, resistance is conferred against soybean cyst nematode (SCN), Heterodera glycines Ichinohe. In this study, we produced transgenic soybean overexpressing GmSAMT1 and characterized their response to various SCN races. Transgenic plants conferred a significant reduction in the development of SCN HG type 1.2.5.7 (race 2), HG type 0 (race 3) and HG type 2.5.7 (race 5). Among transgenic lines, GmSAMT1 expression in roots was positively associated with SCN resistance. In some transgenic lines, there was a significant decrease in salicylic acid titer relative to control plants. No significant seed yield differences were observed between transgenics and control soybean plants grown in one greenhouse with 22 degrees C day/night temperature, whereas transgenic soybean had higher yield than controls grown a warmer greenhouse (27 degrees C day/23 degrees C night) temperature. In a 1-year field experiment in Knoxville, TN, there was no significant difference in seed yield between the transgenic and nontransgenic soybean under conditions with negligible SCN infection. We hypothesize that GmSAMT1 expression affects salicylic acid biosynthesis, which, in turn, attenuates SCN development, without negative consequences to soybean yield or other morphological traits. Thus, we conclude that GmSAMT1 overexpression confers broad resistance to multiple SCN races, which would be potentially applicable to commercial production. C1 [Lin, Jingyu; Mazarei, Mitra; Hatcher, Catherine N.; Wuddineh, Wegi A.; Rudis, Mary; Pantalone, Vincent R.; Hewezi, Tarek; Chen, Feng; Stewart, Charles Neal, Jr.] Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA. [Zhao, Nan; Tschaplinski, Timothy J.] Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN USA. [Arelli, Prakash R.] USDA ARS, Crop Genet Res Unit, Jackson, TN USA. RP Stewart, CN (reprint author), Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA. EM nealstewart@utk.edu OI Hatcher, Catherine N./0000-0002-5502-0557; Tschaplinski, Timothy/0000-0002-9540-6622 FU Tennessee Soybean Promotion Board; Genomic Science Program, U.S. Department of Energy, Office of Science, Biological and Environmental Research, as part of the Plant-Microbe Interfaces Scientific Focus Area at Oak Ridge National Laboratory [DE-AC05-00OR22725] FX This research was supported by the Tennessee Soybean Promotion Board. We would like to thank Dana Pekarchick and Susan Thomas (USDA-ARS, Jackson, TN) and Hollis Rice for maintaining the SCN cultures, Andrew J. Carter for assistance with planting soybeans and isolating cysts, Holly Baxter for taking the photographs of the soybean field, Ben Wolfe and ETREC crew for assistance with the soybean field (University of Tennessee). Thanks to Reginald Millwood for assistance in regulatory matters, including especially the field permit from the USDA APHIS BRS. Co-authors NZ and TJT were also supported by the Genomic Science Program, U.S. Department of Energy, Office of Science, Biological and Environmental Research, as part of the Plant-Microbe Interfaces Scientific Focus Area at Oak Ridge National Laboratory, which is managed by UT-Battelle LLC, for the U.S. Department of Energy under contract DE-AC05-00OR22725. NR 41 TC 0 Z9 0 U1 9 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1467-7644 EI 1467-7652 J9 PLANT BIOTECHNOL J JI Plant Biotechnol. J. PD NOV PY 2016 VL 14 IS 11 BP 2100 EP 2109 DI 10.1111/pbi.12566 PG 10 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA DZ8TB UT WOS:000386143400002 PM 27064027 ER PT J AU Gutierrez-Gonzalez, JJ Garvin, DF AF Gutierrez-Gonzalez, Juan J. Garvin, David F. TI Subgenome-specific assembly of vitamin E biosynthesis genes and expression patterns during seed development provide insight into the evolution of oat genome SO PLANT BIOTECHNOLOGY JOURNAL LA English DT Article DE oat homeologs; vitamin E; homeolog expression; oat evolution; seed composition; tocols ID AVENA-SATIVA; METAGENOMIC DATASETS; MAXIMUM-LIKELIHOOD; SEQUENCES; TRANSCRIPTOME; SUBSTITUTION; PHYLOGENY; MODEL; WHEAT; OIL AB Vitamin E is essential for humans and thus must be a component of a healthy diet. Among the cereal grains, hexaploid oats (Avena sativa L.) have high vitamin E content. To date, no gene sequences in the vitamin E biosynthesis pathway have been reported for oats. Using deep sequencing and orthology-guided assembly, coding sequences of genes for each step in vitamin E synthesis in oats were reconstructed, including resolution of the sequences of homeologs. Three homeologs, presumably representing each of the three oat subgenomes, were identified for the main steps of the pathway. Partial sequences, likely representing pseudogenes, were recovered in some instances as well. Pairwise comparisons among homeologs revealed that two of the three putative subgenome-specific homeologs are almost identical for each gene. Synonymous substitution rates indicate the time of divergence of the two more similar subgenomes from the distinct one at 7.9-8.7MYA, and a divergence between the similar subgenomes from a common ancestor 1.1 MYA. A new proposed evolutionary model for hexaploid oat formation is discussed. Homeolog-specific gene expression was quantified during oat seed development and compared with vitamin E accumulation. Homeolog expression largely appears to be similar for most of genes; however, for some genes, homoeolog-specific transcriptional bias was observed. The expression of HPPD, as well as certain homoeologs of VTE2 and VTE4, is highly correlated with seed vitamin E accumulation. Our findings expand our understanding of oat genome evolution and will assist efforts to modify vitamin E content and composition in oats. C1 [Gutierrez-Gonzalez, Juan J.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Garvin, David F.] USDA ARS, Plant Sci Res Unit, St Paul, MN 55108 USA. RP Gutierrez-Gonzalez, JJ (reprint author), Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA.; Garvin, DF (reprint author), USDA ARS, Plant Sci Res Unit, St Paul, MN 55108 USA. EM jgutierr@umn.edu; david.garvin@ars.usda.gov OI Gutierrez-Gonzalez, Juan/0000-0002-6795-6192 NR 45 TC 0 Z9 0 U1 8 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1467-7644 EI 1467-7652 J9 PLANT BIOTECHNOL J JI Plant Biotechnol. J. PD NOV PY 2016 VL 14 IS 11 BP 2147 EP 2157 DI 10.1111/pbi.12571 PG 11 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA DZ8TB UT WOS:000386143400006 PM 27135276 ER PT J AU Xu, GL Singh, S Barnaby, J Buyer, J Reddy, V Sicher, R AF Xu, Guangli Singh, Shardendu Barnaby, Jinyoung Buyer, Jeffrey Reddy, Vangimalla Sicher, Richard TI Effects of growth temperature and carbon dioxide enrichment on soybean seed components at different stages of development SO PLANT PHYSIOLOGY AND BIOCHEMISTRY LA English DT Article DE Glycine max; Seed protein; Primary metabolism; Seed development; Heat stress; Oil seeds ID AMINO-ACIDS; CO2 ENRICHMENT; ORGANIC-ACIDS; ELEVATED CO2; PROTEIN; OIL; PHOTOSYNTHESIS; PHOTOPERIOD; QUALITY; ENZYMES AB Soybean plants were grown to maturity in controlled environment chambers and at the onset of flowering three temperature treatments were imposed that provided optimum [28/24 degrees C], low [22/18 degrees C] or high [36/32 degrees C] chamber air temperatures. In addition, plants were treated continuously with either 400 or 800 mu mol mol(-1) CO2. Seeds were harvested at 42, 53, 69 and 95 days after planting (i.e., final maturity). This study quantified 51 metabolites in developing soybean seeds, plus total lipids and proteins were measured at maturity. About 80% of measured soluble carbohydrates, amines and organic acids decreased to low levels in mature seeds, although important exceptions were raffinose, ribose/ arabinose, citrate and all eight fatty acids. This suggested that the metabolism of young seeds supported lipid and protein synthesis. A total of 35 and 9 metabolites differed among temperature and CO2 treatments, respectively, and treatment effects were predominately observed on the first and second samplings. However, shikimate, pinitol and oleate were increased by high temperature treatments in mature seeds. The above results indicated that CO2 enrichment primarily altered metabolite levels during the initial stages of seed development and this was likely due to enhanced photosynthate formation in leaves. Published by Elsevier Masson SAS. C1 [Xu, Guangli; Singh, Shardendu; Barnaby, Jinyoung; Reddy, Vangimalla; Sicher, Richard] USDA ARS, Crop Syst & Climate Change Lab, Room 342,Bldg 001,BARC West,10300 Baltimore Ave, Beltsville, MD 20705 USA. [Buyer, Jeffrey] USDA ARS, Sustainable Agr Syst Lab, Room 245,Bldg 001,BARC West,10300 Baltimore Ave, Beltsville, MD 20705 USA. [Xu, Guangli] Sichuan Agr Univ, Coll Agron, 211 Huimin Rd, Chengdu 611130, Peoples R China. RP Sicher, R (reprint author), USDA ARS, Crop Syst & Climate Change Lab, Room 342,Bldg 001,BARC West,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM richard.sicher@ars.usda.gov NR 35 TC 0 Z9 0 U1 14 U2 14 PU ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER PI PARIS PA 23 RUE LINOIS, 75724 PARIS, FRANCE SN 0981-9428 J9 PLANT PHYSIOL BIOCH JI Plant Physiol. Biochem. PD NOV PY 2016 VL 108 BP 313 EP 322 DI 10.1016/j.plaphy.2016.07.025 PG 10 WC Plant Sciences SC Plant Sciences GA EA9UH UT WOS:000386989700033 PM 27494565 ER PT J AU Ge, LF Yu, JB Wang, HL Luth, D Bai, GH Wang, K Chen, RJ AF Ge, Liangfa Yu, Jianbin Wang, Hongliang Luth, Diane Bai, Guihua Wang, Kan Chen, Rujin TI Increasing seed size and quality by manipulating BIG SEEDS1 in legume species SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE plant organ size; seed size; forage quality; Medicago; soybean ID MEDICAGO-TRUNCATULA; TRANSCRIPTION FACTOR; LEAF DEVELOPMENT; CELL-PROLIFERATION; ARABIDOPSIS; FAMILY; GROWTH; DIFFERENTIATION; EMBRYOGENESIS; CURVATURE AB Plant organs, such as seeds, are primary sources of food for both humans and animals. Seed size is one of the major agronomic traits that have been selected in crop plants during their domestication. Legume seeds are a major source of dietary proteins and oils. Here, we report a conserved role for the BIG SEEDS1 (BS1) gene in the control of seed size and weight in the model legume Medicago truncatula and the grain legume soybean (Glycine max). BS1 encodes a plant-specific transcription regulator and plays a key role in the control of the size of plant organs, including seeds, seed pods, and leaves, through a regulatory module that targets primary cell proliferation. Importantly, down-regulation of BS1 orthologs in soybean by an artificial microRNA significantly increased soybean seed size, weight, and amino acid content. Our results provide a strategy for the increase in yield and seed quality in legumes. C1 [Ge, Liangfa; Wang, Hongliang; Chen, Rujin] Samuel Roberts Noble Fdn Inc, Plant Biol Div, Ardmore, OK 73401 USA. [Yu, Jianbin; Bai, Guihua] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Luth, Diane; Wang, Kan] Iowa State Univ, Inst Plant Sci, Ctr Plant Transformat, Ames, IA 50011 USA. [Luth, Diane; Wang, Kan] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Bai, Guihua] ARS, Hard Winter Wheat Genet Res Unit, USDA, Manhattan, KS 66506 USA. [Yu, Jianbin] Pioneer HiBred Int Inc, Data Sci & Informat, Willmar, MN 56201 USA. [Wang, Hongliang] ARS, Crop Genet & Breeding Res, USDA, Tifton, GA 31793 USA. RP Chen, RJ (reprint author), Samuel Roberts Noble Fdn Inc, Plant Biol Div, Ardmore, OK 73401 USA. EM rchen@noble.org RI Ge, Liangfa/F-2110-2016 OI Ge, Liangfa/0000-0002-4831-4637 FU Samuel Roberts Noble Foundation; National Science Foundation [IOS-1127155] FX We thank Randy Allen (Oklahoma State University) for critical reading of the manuscript, Guifen Li and Yuhong Tang (Noble Foundation) for assistance with RNA in situ and microarray experiments, Jing Li and David Human (Noble Foundation) for fatty acid measurements, and Jay Gambee (Amino Acid Analysis Service Laboratory, Inc.) for amino acid analysis. Funding of this work was provided by the Samuel Roberts Noble Foundation. Development of the Medicago FNB mutant resources used in this study was funded by National Science Foundation Grant IOS-1127155 (to R.C.). NR 34 TC 0 Z9 0 U1 6 U2 6 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD NOV 1 PY 2016 VL 113 IS 44 BP 12414 EP 12419 DI 10.1073/pnas.1611763113 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EA4TR UT WOS:000386608200042 PM 27791139 ER PT J AU Zhang, B Tieman, DM Jiao, C Xu, YM Chen, KS Fe, ZJ Giovannoni, JJ Klee, HJ AF Zhang, Bo Tieman, Denise M. Jiao, Chen Xu, Yimin Chen, Kunsong Fe, Zhangjun Giovannoni, James J. Klee, Harry J. TI Chilling-induced tomato flavor loss is associated with altered volatile synthesis and transient changes in DNA methylation SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE fruit quality; methylome; transcriptome ID CAROTENOID OXIDATION-PRODUCTS; COLD RESPONSE PATHWAY; SOLANUM-LYCOPERSICON; STORAGE-TEMPERATURE; QUALITY ATTRIBUTES; STRESS SIGNALS; SYNTHASE GENE; CBF REGULON; PLANTS; IDENTIFICATION AB Commercial tomatoes are widely perceived by consumers as lacking flavor. A major part of that problem is a postharvest handling system that chills fruit. Low-temperature storage is widely used to slow ripening and reduce decay. However, chilling results in loss of flavor. Flavor-associated volatiles are sensitive to temperatures below 12 degrees C, and their loss greatly reduces flavor quality. Here, we provide a comprehensive view of the effects of chilling on flavor and volatiles associated with consumer liking. Reduced levels of specific volatiles are associated with significant reductions in transcripts encoding key volatile synthesis enzymes. Although expression of some genes critical to volatile synthesis recovers after a return to 20 degrees C, some genes do not. RNAs encoding transcription factors essential for ripening, including RIPENING INHIBITOR (RIN), NONRIPENING, and COLORLESS NONRIPENING are reduced in response to chilling and may be responsible for reduced transcript levels in many downstream genes during chilling. Those reductions are accompanied by major changes in the methylation status of promoters, including RIN. Methylation changes are transient and may contribute to the fidelity of gene expression required to provide maximal beneficial environmental response with minimal tangential influence on broader fruit developmental biology. C1 [Zhang, Bo; Tieman, Denise M.; Klee, Harry J.] Univ Florida, Genet Inst, Plant Innovat Ctr, Hort Sci, Gainesville, FL 32611 USA. [Zhang, Bo; Chen, Kunsong] Zhejiang Univ, Lab Fruit Qual Biol, Zhejiang Prov Key Lab Hort Plant Integrat Biol, Hangzhou 310058, Zhejiang, Peoples R China. [Jiao, Chen; Xu, Yimin; Fe, Zhangjun; Giovannoni, James J.] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Jiao, Chen; Xu, Yimin; Fe, Zhangjun; Giovannoni, James J.] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Klee, HJ (reprint author), Univ Florida, Genet Inst, Plant Innovat Ctr, Hort Sci, Gainesville, FL 32611 USA. EM hjklee@ufl.edu FU National Science Foundation [IOS-0923312]; National Key Research and Development Program [2016YFD0400101]; Program of International Science and Technology Cooperation [2011DFB31580]; New Star Program from Zhejiang University; China Scholarship Council FX We thank Drs. Charles Sims and Asli Odabasi for their help with the consumer panel; Dawn Bies for help with volatile collection; Mark Taylor for help with plant care; and Michael Thomashow for invaluable advice. This work was supported by National Science Foundation Grants IOS-0923312 (to H.J.K., J.J.G., and Z.F.); National Key Research and Development Program 2016YFD0400101; Program of International Science and Technology Cooperation Grant 2011DFB31580; the New Star Program from Zhejiang University; and the China Scholarship Council. NR 49 TC 2 Z9 2 U1 37 U2 37 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD NOV 1 PY 2016 VL 113 IS 44 BP 12580 EP 12585 DI 10.1073/pnas.1613910113 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EA4TR UT WOS:000386608200070 PM 27791156 ER PT J AU Li, L Shi, X Zheng, F Li, CC Wu, D Bai, GH Gao, DR Wu, JC Li, T AF Li, Lei Shi, Xuan Zheng, Fei Li, Changcheng Wu, Di Bai, Guihua Gao, Derong Wu, Jincai Li, Tao TI A novel nitrogen-dependent gene associates with the lesion mimic trait in wheat SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID CELL-DEATH; HYPERSENSITIVE RESPONSE; ARABIDOPSIS-THALIANA; RNA-SEQ; ENHANCED RESISTANCE; FEEDBACK-REGULATION; NITRIC-OXIDE; LEAF RUST; MUTANTS; RICE AB Using bulk segregant analysis (BSA) coupling with RNA-seq and DNA markers identified a potentially novel nitrogen-dependent lesion mimic gene Ndhrl1 on 2BS in wheat. Lesion mimic (LM) refers to hypersensitive reaction-like (HRL) traits that appear on leaf tissue in the absence of plant pathogens. In a wheat line P7001, LM showed up on the leaves under the 0 g nitrogen (N) treatment, but disappeared when sufficient N was supplied, suggesting that LM is N-responsive and N dosage dependent. Using BSA strategy together with RNA-seq and DNA markers, we identified an N-dependent LM gene (Ndhrl1) and mapped it to the short arm of chromosome 2B using an F-5 recombinant inbred population developed from the cross of P7001 x P216. The putative gene was delimited into an interval of 8.1 cM flanked by the CAPS/dCAPS markers 7hrC9 and 7hr2dc14, and co-segregated with the dCAPS marker 7hrdc2. This gene is most likely a novel gene for LM in wheat based on its chromosomal location. Further analysis of RNA-seq data showed that plant-pathogen interaction, nitrogen metabolism, zeatin biosynthesis and plant hormone signal transduction pathways were significantly differentially expressed between LM and non-LM lines. C1 [Li, Lei; Shi, Xuan; Zheng, Fei; Li, Changcheng; Wu, Di; Li, Tao] Yangzhou Univ, Jiangsu Prov Key Lab Crop Genet & Physiol, Coinnovat Ctr Modern Prod Technol Grain Crops, Key Lab Plant Funct Genom,Minist Educ,Wheat Res C, Yangzhou 225009, Jiangsu, Peoples R China. [Li, Lei; Wu, Jincai] Yangzhou Univ, Coll Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China. [Bai, Guihua] Lixiahe Agr Res Inst Jiangsu Prov, Yangzhou 225000, Jiangsu, Peoples R China. [Bai, Guihua] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Gao, Derong] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. RP Li, T (reprint author), Yangzhou Univ, Jiangsu Prov Key Lab Crop Genet & Physiol, Coinnovat Ctr Modern Prod Technol Grain Crops, Key Lab Plant Funct Genom,Minist Educ,Wheat Res C, Yangzhou 225009, Jiangsu, Peoples R China. EM taoli@yzu.edu.cn FU National Natural Science Foundation of China [31270704]; Vital Project of Natural Science of Universities in Jiangsu Province [12KJA210002]; China Postdoctoral Science Foundation [2014M560449]; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) FX We thank the financial support provided by the National Natural Science Foundation of China (31270704), the Vital Project of Natural Science of Universities in Jiangsu Province (12KJA210002), the China Postdoctoral Science Foundation (2014M560449), and by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). Our thanks also go to Dr. Mei Han and Dr. Tao Su for improving English language of this manuscript. NR 51 TC 0 Z9 0 U1 11 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD NOV PY 2016 VL 129 IS 11 BP 2075 EP 2084 DI 10.1007/s00122-016-2758-3 PG 10 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA EA4FJ UT WOS:000386565400004 PM 27460590 ER EF