FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Wilensky, RL Lehmann, KG Werner, JA Chambers, JW Harlamert, E Dillon, JC AF Wilensky, RL Lehmann, KG Werner, JA Chambers, JW Harlamert, E Dillon, JC TI The Biostent trial: A randomized, double-blind, prospective, multi-center trial evaluating the safety and tolerability of locally delivered cytochalasin B for prophylaxis of angioplasty restenosis SO CIRCULATION LA English DT Meeting Abstract C1 UNIV PENN,PHILADELPHIA,PA 19104. VA PUGET SOUND HLTH CARE SYST,SEATTLE,WA. PROVIDENCE HEART GRP,SEATTLE,WA. UNIV WASHINGTON,SEATTLE,WA 98195. COMMUNITY HOSP E,INDIANAPOLIS,IN. INDIANA UNIV,INDIANAPOLIS,IN 46204. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD OCT 21 PY 1997 VL 96 IS 8 SU S BP 3641 EP 3641 PG 1 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA YC880 UT WOS:A1997YC88003622 ER PT J AU Crawford, MH Ferry, DR Deedwania, PC Pepine, CJ Lehmann, KG Boden, WE AF Crawford, MH Ferry, DR Deedwania, PC Pepine, CJ Lehmann, KG Boden, WE TI Complications of revascularization in non Q-wave myocardial infarction patients: Results of the VA trial SO CIRCULATION LA English DT Meeting Abstract C1 UNIV NEW MEXICO,ALBUQUERQUE,NM 87131. VET ADM MED CTR,FRESNO,CA. UNIV FLORIDA,GAINESVILLE,FL. VA PUGET SOUND HLTH CARE SYST,SEATTLE,WA. VET ADM MED CTR,SYRACUSE,NY. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD OCT 21 PY 1997 VL 96 IS 8 SU S BP 3919 EP 3919 PG 1 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA YC880 UT WOS:A1997YC88003895 ER PT J AU Michaels, AD Barron, HV Maynard, C Every, NR AF Michaels, AD Barron, HV Maynard, C Every, NR TI Clinical predictors of ACE inhibitor use after acute myocardial infarction: Experience from the Second National Registry of Myocardial Infarction (NRMI 2) SO CIRCULATION LA English DT Meeting Abstract C1 UNIV CALIF SAN FRANCISCO,SAN FRANCISCO,CA 94143. VA PUGET SOUND HLTH CARE SYST,SEATTLE,WA. UNIV WASHINGTON,SEATTLE,WA 98195. RI Maynard, Charles/N-3906-2015 OI Maynard, Charles/0000-0002-1644-7814 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD OCT 21 PY 1997 VL 96 IS 8 SU S BP 3950 EP 3950 PG 2 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA YC880 UT WOS:A1997YC88003926 ER PT J AU Doranz, BJ GrovitFerbas, K Sharron, MP Mao, SH Goetz, MB Daar, ES Doms, RW OBrien, WA AF Doranz, BJ GrovitFerbas, K Sharron, MP Mao, SH Goetz, MB Daar, ES Doms, RW OBrien, WA TI A small-molecule inhibitor directed against the chemokine receptor CXCR4 prevents its use as an HIV-1 coreceptor SO JOURNAL OF EXPERIMENTAL MEDICINE LA English DT Article ID IMMUNODEFICIENCY-VIRUS TYPE-1; INFECTION; ENTRY; INDIVIDUALS; CCR5; GENE; MIP-1-ALPHA; PROGRESSION; LESTR/FUSIN; REPLICATION AB The chemokine receptor CXCR4 is the major coreceptor used for cellular entry by T cell-tropic human immunodeficiency virus (HIV)-1 strains, whereas CCR5 is used by macrophage (M)-tropic strains. Here we show that a small-molecule inhibitor, ALX40-4C, inhibits HIV-1 envelope (Env)-mediated membrane fusion and viral entry directly at the level of coreceptor use. ALX40-4C inhibited HIV-1 use of the coreceptor CXCR4 by T-and dual-tropic HIV-1 strains, whereas use of CCR5 by M-and dual-tropic strains was not inhibited. Dual-tropic viruses capable of using both CXCR4 and CCR5 were inhibited by ALX40-4C only when cells expressed CXCR4 alone. ALX40-4C blocked stromal-derived factor (SDF)-1 alpha-mediated activation of CXCR4 and binding of the monoclonal antibody 12G5 to cells expressing CXCR4. Overlap of the ALX40-4C binding site with that of 12G5 and SDF implicates direct blocking of Env interactions, rather than downregulation of receptor, as the mechanism of inhibition. Thus, ALX40-4C represents a small-molecule inhibitor of HIV-1 infection that acts directly against a chemokine receptor at the level of Env-mediated membrane fusion. C1 UNIV TEXAS,MED BRANCH,DEPT MED,GALVESTON,TX 77555. UNIV PENN,DEPT PATHOL & LAB MED,PHILADELPHIA,PA 19104. W LOS ANGELES VET AFFAIRS MED CTR,DEPT MED,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. CEDARS SINAI MED CTR,LOS ANGELES,CA 90048. OI Goetz, Matthew/0000-0003-4542-992X FU NIAID NIH HHS [AI-38225, AI-40880, R01 AI040880, T32 AI007388]; NINDS NIH HHS [NS-35705] NR 34 TC 290 Z9 295 U1 0 U2 1 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 SN 0022-1007 J9 J EXP MED JI J. Exp. Med. PD OCT 20 PY 1997 VL 186 IS 8 BP 1395 EP 1400 DI 10.1084/jem.186.8.1395 PG 6 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA YC950 UT WOS:A1997YC95000025 PM 9334380 ER PT J AU Buchsbaum, MS Trestman, RL Hazlett, E Siegel, BV Schaefer, CH LuuHsia, C Tang, C Herrera, S Solimando, AC Losonczy, M Serby, M Silverman, J Siever, LJ AF Buchsbaum, MS Trestman, RL Hazlett, E Siegel, BV Schaefer, CH LuuHsia, C Tang, C Herrera, S Solimando, AC Losonczy, M Serby, M Silverman, J Siever, LJ TI Regional cerebral blood flow during the Wisconsin Card Sort Test in schizotypal personality disorder SO SCHIZOPHRENIA RESEARCH LA English DT Article DE single photon emission computed tomography; frontal lobe; neuropsychology; schizophrenia ID DORSOLATERAL PREFRONTAL CORTEX; HIPPOCAMPAL PYRAMIDAL CELL; FRONTAL-LOBE LESIONS; SCHIZOPHRENIC-PATIENTS; PHYSIOLOGICAL DYSFUNCTION; TEST-PERFORMANCE; ACTIVATION; SPECT; ABNORMALITIES; AMPHETAMINE AB Regional cerebral blood flow (rCBF) was measured by single photon emission computed tomography in 10 patients with schizotypal personality disorder (SPD) and nine age-and sex-matched normal volunteers. Subjects performed both the Wisconsin Card Sort Test (WCST) and a control task, the Symbol Matching Test (SMT). Four-way analyses of variance were performed to assess relative rCBF of the prefrontal cortex and of the medial temporal region. Normal volunteers showed more marked activation in the precentral gyrus, while SPD patients showed greater activation in the middle frontal gyrus. Relative flow in the left prefrontal cortex was correlated with better WCST performance in normal volunteers. SPD patients, however, showed no such correlations in the left prefrontal cortex, but demonstrated correlations of good and bad performance with CBF in the right middle and inferior frontal gyrus, respectively. Thus, at least some SPD patients demonstrate abnormal patterns of prefrontal activation, perhaps as a compensation for dysfunction in other regions. (C) 1997 Elsevier Science B.V. C1 UNIV CALIF LOS ANGELES,DEPT PSYCHIAT,LOS ANGELES,CA 90073. W LOS ANGELES DEPT VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. BRONX VET AFFAIRS MED CTR,DEPT PSYCHIAT,BRONX,NY 10468. BRONX VET AFFAIRS MED CTR,DEPT NUCL MED,BRONX,NY 10468. FDR VET AFFAIRS MED CTR,PSYCHIAT SERV,MONTROSE,NY 10548. RP Buchsbaum, MS (reprint author), CUNY MT SINAI SCH MED,DEPT PSYCHIAT,BOX 1505,1 GUSTAVE L LEVY PL,NEW YORK,NY 10029, USA. FU NIMH NIH HHS [MH40071, 5R37MH42827-02] NR 44 TC 44 Z9 44 U1 1 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD OCT 17 PY 1997 VL 27 IS 1 BP 21 EP 28 DI 10.1016/S0920-9964(97)00081-9 PG 8 WC Psychiatry SC Psychiatry GA YD369 UT WOS:A1997YD36900003 PM 9373891 ER PT J AU Buchsbaum, MS Yang, S Hazlett, E Siegel, BV Germans, M Haznedar, M OFlaithbheartaigh, S Wei, TC Silverman, J Siever, LJ AF Buchsbaum, MS Yang, S Hazlett, E Siegel, BV Germans, M Haznedar, M OFlaithbheartaigh, S Wei, TC Silverman, J Siever, LJ TI Ventricular volume and asymmetry in schizotypal personality disorder and schizophrenia assessed with magnetic resonance imaging SO SCHIZOPHRENIA RESEARCH LA English DT Article DE temporal horn; laterality; ventricle-brain ratio; memory; duration of illness ID DEVELOPMENTAL BRAIN ABNORMALITIES; DORSOLATERAL PREFRONTAL CORTEX; STRUCTURED INTERVIEW; COMPUTED-TOMOGRAPHY; ENLARGEMENT; DEFICITS; MOTHERS; RISK; DYSFUNCTION; DIAGNOSIS AB Magnetic resonance imaging (MRI) was performed in 12 patients with schizotypal personality disorder (SPD), 11 patients with chronic schizophrenia, and 23 age- and sex-matched normal volunteers. MRI slices were acquired in the axial plane at 1.2-mm intervals, and the ventricles were traced on every other slice. The lateral ventricular system was divided into the anterior horn, temporal horn, and dorsal lateral ventricle. Schizophrenic patients had larger left anterior and temporal horns than the normal volunteers. Size of the left anterior and temporal horn in SPD patients was intermediate between those of normal volunteers and schizophrenic patients and differed significantly from schizophrenic patients. The left-minus-right difference was larger in schizophrenic patients than in normal volunteers or SPD patients. Thus, in their structural brain characteristics, as well as in their clinical symptomatology, SPD patients evidence, in attenuated form, abnormalities resembling those found in full-ledged schizophrenia. The findings suggest that decreased left hemispheric volume, in frontal and temporal regions, may characterize both psychotic and non-psychotic disorders of the schizophrenia spectrum. (C) 1997 Elsevier Science B.V. C1 UNIV CALIF LOS ANGELES,DEPT PSYCHIAT,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. DVA MED CTR,PSYCHIAT SERV,BRONX,NY 10468. RP Buchsbaum, MS (reprint author), CUNY MT SINAI SCH MED,DEPT PSYCHIAT,BOX 1505,1 GUSTAVE L LEVY PL,NEW YORK,NY 10029, USA. FU NIMH NIH HHS [MH48858, MH42827, MH40071] NR 43 TC 82 Z9 82 U1 1 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD OCT 17 PY 1997 VL 27 IS 1 BP 45 EP 53 DI 10.1016/S0920-9964(97)00087-X PG 9 WC Psychiatry SC Psychiatry GA YD369 UT WOS:A1997YD36900006 PM 9373894 ER PT J AU McHorney, CA AF McHorney, CA TI Generic health measurement: Past accomplishments and a measurement paradigm for the 21st century SO ANNALS OF INTERNAL MEDICINE LA English DT Review ID SICKNESS IMPACT PROFILE; QUALITY-OF-LIFE; FUNCTIONAL STATUS; RASCH ANALYSIS; PRIMARY CARE; RHEUMATOID-ARTHRITIS; POPULATION SURVEY; ITEM SELECTION; RESPONSE RATES; SURVEY SF-36 AB Generic health surveys have been proposed for use in increasingly diverse applications and populations. This paper describes the history of generic tools in the past 30 years and suggests a more modern measurement platform for advances in the 21st century. Many generic tools lack the precision required for effective health care decision making. A meaningful goal for the next era of development of generic measures should be the generation of equiprecise measurement for generic health concepts. Equiprecise tests yield measures of equal precision at all levels of the underlying construct. Equiprecise measurement can be achieved through conjoint use of computerized-adaptive testing as the survey platform and item response theory as the measurement theory. C1 UNIV WISCONSIN,SCH MED,MADISON,WI. RP McHorney, CA (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,PATHOL & LAB MED SERV,2500 OVERLOOK TERRACE,MADISON,WI 53705, USA. NR 102 TC 153 Z9 155 U1 23 U2 29 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD OCT 15 PY 1997 VL 127 IS 8 BP 743 EP 750 PN 2 PG 8 WC Medicine, General & Internal SC General & Internal Medicine GA YB385 UT WOS:A1997YB38500016 PM 9382391 ER PT J AU Roodman, GD AF Roodman, GD TI Mechanisms of bone lesions in multiple myeloma and lymphoma SO CANCER LA English DT Article; Proceedings Paper CT Symposium on Skeletal Complications of Malignancy CY APR 19-20, 1997 CL NIH, NATCHER CTR, BETHESDA, MD SP Paget Fdn HO NIH, NATCHER CTR DE myeloma; lymphoma; 1,25-(OH)(2)D-3; parathyroid hormone related protein; bone lesions; interleukin-1 ID T-CELL LEUKEMIA; HORMONE-RELATED PROTEIN; MARROW STROMAL CELLS; INTERLEUKIN-6 SECRETION; ADHESION MOLECULES; PAGETS-DISEASE; GIANT-CELLS; TUMOR-CELLS; HYPERCALCEMIA; RESORPTION AB BACKGROUND. Bone lesions and hypercalcemia occur rarely in patients with hematologic malignancies, except those patients with multiple myeloma and adult T-cell leukemia/lymphoma (ATL) associated with the human T-cell leukemia/lymphoma virus-1 (HTLV-1) virus. The primary mechanism for bone destruction in patients with myeloma and lymphoma is increased osteoclastic bone resorption. In patients with multiple myeloma, new bone formation is also inhibited. Mediators including lymphotoxin, interleukin-1 beta, parathyroid hormone related protein (PTHrP), and interleukin-6, produced by the myeloma cells or by marrow stromal cells in response to myeloma cells, have been implicated as osteoclast-activating factors (OAF) in multiple myeloma. However, most studies to identify OAF produced by myeloma cells have been inconclusive. METHODS. To try to identify the OAF produced by myeloma cells, we developed an in vivo model of human myeloma bone disease using the ARH-77 myeloma cell line transplanted into severe combined immunodeficiency mice. RESULTS. We found that a novel cytokine(s) may be responsible for bone destruction. Interleukin-1 and PTHrP mediate bone destruction in patients with ATL. These factors can be detected in media conditioned by ATL cells or by lymphocytes infected with HTLV-1. Furthermore, serum PTHrP levels are increased in ATL patients. In patients with Hodgkin's disease or other types of non-Hodgkin's lymphoma, 1,25-(OH)(2)D-3 or PTHrP is produced by the lymphoma cells and mediates bone destruction. Chemotherapy or resection of the lymphoma decreases 1,25-(OH)2D(3) levels and hypercalcemia in these patients. CONCLUSION. Thus, OAF produced locally by the tumor or the marrow microenvironment play an important role in the bone destruction seen in patients with hematologic malignancies. (C) 1997 American Cancer Society. C1 AUDIE L MURPHY MEM VET ADM MED CTR,DEPT MED HEMATOL,SAN ANTONIO,TX 78284. FU NCI NIH HHS [CA 69136-01A1] NR 38 TC 117 Z9 119 U1 0 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0008-543X J9 CANCER JI Cancer PD OCT 15 PY 1997 VL 80 IS 8 SU S BP 1557 EP 1563 DI 10.1002/(SICI)1097-0142(19971015)80:8+<1557::AID-CNCR5>3.3.CO;2-K PG 7 WC Oncology SC Oncology GA YA765 UT WOS:A1997YA76500006 PM 9362422 ER PT J AU Berenson, JR AF Berenson, JR TI Bisphosphonates in multiple myeloma SO CANCER LA English DT Article; Proceedings Paper CT Symposium on Skeletal Complications of Malignancy CY APR 19-20, 1997 CL NIH, NATCHER CTR, BETHESDA, MD SP Paget Fdn HO NIH, NATCHER CTR DE multiple myeloma; bisphosphonates; etidronate; clodronate; pamidronate ID BREAST-CANCER; BONE METASTASES; MULTICENTER TRIAL; IN-VIVO; PAMIDRONATE; CLODRONATE; RESORPTION; PLACEBO AB The major clinical manifestations of multiple myeloma are related to enhanced bone destruction resulting in osteolytic lesions, osteoporosis, and pathologic fractures in most patients as well as hypercalcemia and spinal cord compression in many individuals. These patients frequently require radiation therapy or surgery. In an attempt to reduce these complications, bisphosphonates have been evaluated in several large randomized trials in patients also receiving chemotherapy. Oral etidronate given daily showed no clinical benefit, whereas the use of oral clodronate daily did reduce the development of new osteolytic lesions but did not significantly affect bone pain or rates of pathologic fractures. A large, randomized, double-blind study was conducted in which Stage III multiple myeloma patients received either pamidronate (90 mg) or placebo as a 4-hour infusion every 4 weeks for 21 cycles in addition to antimyeloma chemotherapy. The proportion of patients with at least one skeletal complication was significantly reduced in the pamidronate group compared with the placebo group. Although survival was not different between the pamidronate and placebo groups overall, patients in whom first-line chemotherapy had failed when they entered the trial lived longer with pamidronate treatment than those receiving placebo. Patients who received pamidronate had significant decreases in bone pain, had less analgesic drug use, and had better Eastern Cooperative Oncology Group performance status than patients receiving placebo. Pamidronate was safe and well tolerated during the trial. (C) 1997 American Cancer Society. C1 UNIV CALIF LOS ANGELES,JONSSON COMPREHENS CANC CTR,SCH MED,LOS ANGELES,CA 90024. RP Berenson, JR (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,DIV MED ONCOL,111H,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 28 TC 27 Z9 27 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0008-543X J9 CANCER JI Cancer PD OCT 15 PY 1997 VL 80 IS 8 SU S BP 1661 EP 1667 PG 7 WC Oncology SC Oncology GA YA765 UT WOS:A1997YA76500017 PM 9362433 ER PT J AU Quinn, LS Haugk, KL Damon, SE AF Quinn, LS Haugk, KL Damon, SE TI Interleukin-15 stimulates C2 skeletal myoblast differentiation SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID FACTOR-BINDING-PROTEIN; GROWTH FACTOR-II; IL-2 RECEPTOR; BETA-CHAIN; EXPRESSION; MUSCLE; PROLIFERATION; CLONING AB Interleukin-15 (IL-15) is a cytokine which is highly expressed in skeletal muscle, and which stimulates muscle protein accretion in cultured skeletal muscle fibers. Using parental C2 skeletal myoblasts, no significant effects of IL-15 on skeletal muscle differentiation were observed. To test the hypothesis that IL-15 may stimulate skeletal muscle differentiation if the strong differentiation-inducing effects of autocrine insulin-like growth factor (IGF) production were inhibited, a C2 myoblast subline (C2-pBP4) was stably transfected with an expression vector for rat IGF binding protein-4 (IGFBP-4). Differentiation responses to autocrine and exogenous IGFs in C2-BP4 myoblasts were reduced 3- to 4-fold in C2-BP4 cultures compared to C2-pLXSN cultures, a subline transfected with a control plasmid. Addition of IL-15 to C2-pBP4 myoblasts doubled the number of differentiated muscle cells which arose. These findings indicate that IL-15 can stimulate myogenic differentiation in conditions in which the strongly differentiative effects of the IGFs are inhibited. The differentiative activity of IL-15 may be of physiological significance in conditions in which IGF concentrations are low or in which the IGFs are sequestered by binding proteins. (C) 1997 Academic Press. C1 UNIV WASHINGTON,DEPT MED,SEATTLE,WA 98195. RP Quinn, LS (reprint author), VA PUGET SOUND HLTH CARE SYST,CTR GERIATR RES EDUC & CLIN,TACOMA,WA 98493, USA. FU NIA NIH HHS [AG 13280]; NICHD NIH HHS [N01-HD-6-2915] NR 18 TC 34 Z9 36 U1 0 U2 4 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD OCT 9 PY 1997 VL 239 IS 1 BP 6 EP 10 DI 10.1006/bbrc.1997.7414 PG 5 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA YB131 UT WOS:A1997YB13100002 PM 9345260 ER PT J AU Navder, KP Baraona, E Lieber, CS AF Navder, KP Baraona, E Lieber, CS TI Polyenylphosphatidylcholine decreases alcoholic hyperlipemia without affecting the alcohol-induced rise of HDL-cholesterol SO LIFE SCIENCES LA English DT Article DE polyenylphosphatidylcholine; alcohol; serum lipoproteins; rats ID HYPERCHOLESTEROLEMIC RATS; POLYUNSATURATED LECITHIN; PLASMA-CHOLESTEROL; SERUM-CHOLESTEROL; CORN-OIL; LIPOPROTEINS; METABOLISM; ABSORPTION; LIPIDS; PATHOGENESIS AB In ethanol-fed rats, supplementation of the diet with soybean polyenylphosphatidylcholine (3 g/liter for 21 days) markedly decreased postprandial VLDL-triglycerides and both VLDL- and LDL-cholesterol levels, whereas it maintained high levels of HDL-cholesterol, compared to an equivalent intake of choline and polyunsaturated fatty acids. By contrast, there were no changes in the serum lipoproteins of the pair-fed controls. The prevention of alcoholic hypertriglyceridemia was associated with marked attenuation of the alcoholic fatty liver and it occurred despite a slight increase in fat absorption. Thus, the administration of polyenylphosphatidylcholine not only attenuates the hepatotoxicity of ethanol, but also increases the HDL/LDL cholesterol ratio, which may be beneficial for the prevention of atherosclerosis and coronary heart disease. C1 BRONX VET AFFAIRS MED CTR,CTR ALCOHOL RES & TREATMENT,BRONX,NY 10468. MT SINAI SCH MED,BRONX,NY 10468. CUNY HUNTER COLL,DEPT FOOD SCI & NUTR,NEW YORK,NY 10010. FU NIAAA NIH HHS [AA05934, AA11115] NR 41 TC 19 Z9 22 U1 1 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0024-3205 J9 LIFE SCI JI Life Sci. PD OCT 3 PY 1997 VL 61 IS 19 BP 1907 EP 1914 DI 10.1016/S0024-3205(97)00830-8 PG 8 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA YA565 UT WOS:A1997YA56500005 PM 9364195 ER PT J AU Kiesz, RS Rozek, MM Mego, DM Miller, LA OLeary, E Patel, V SaundersWebb, E Ebersole, DG Chilton, R AF Kiesz, RS Rozek, MM Mego, DM Miller, LA OLeary, E Patel, V SaundersWebb, E Ebersole, DG Chilton, R TI Acute directional coronary atherectomy prior to stenting: Long term angiographic results. SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,S TEXAS VET HLTH SYST,AUDIE MURPHY DIV,SAN ANTONIO,TX. BROOKE ARMY MED CTR,SAN ANTONIO,TX. NR 0 TC 0 Z9 0 U1 0 U2 0 PU EXCERPTA MEDICA INC PI NEW YORK PA 245 WEST 17TH STREET, NEW YORK, NY 10011 SN 0002-9149 J9 AM J CARDIOL JI Am. J. Cardiol. PD OCT PY 1997 VL 80 SI 7A BP TCT83 EP TCT83 PG 1 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA YC885 UT WOS:A1997YC88500083 ER PT J AU Bridges, AJ Lorden, TE Havighurst, TC AF Bridges, AJ Lorden, TE Havighurst, TC TI Autoantibody testing for connective tissue diseases - Comparison of immunodiffusion, immunoblot, and enzyme immunoassay SO AMERICAN JOURNAL OF CLINICAL PATHOLOGY LA English DT Article DE autoantibody; enzyme immunoassay; immunoblot; connective tissue disease; systemic lupus erythematosus; Sjogren's syndrome ID SYSTEMIC LUPUS-ERYTHEMATOSUS; AUTOIMMUNE-DISEASES; REVISED CRITERIA; ANTIBODIES; CLASSIFICATION; ELISA AB We evaluated 500 consecutive patient serum samples for the presence of six autoantibodies by three antibody detection methods: immunodiffusion, immunoblot, and enzyme immunoassay. Clinical data were reviewed for each patient with positive antibody test results. Serum samples from 60 patients revealed antibodies to Sm, ribonucleoprotein (RNP), SSA/Ro, SSB/La, Scl-70, or Jo-1. There were 7 false-positive test results (1%). All three methods detected autoantibodies in 36 (68%) of 53 patients with connective tissue disease. Immunoblot was the most sensitive method to detect autoantibodies (92%). Enzyme immunoassay and immunodiffusion were less sensitive (81% and 74%, respectively). Antiribonucleoprotein and anti-SSB/La antibodies were more often detected by immunoblotting, whereas anti-SSA/Ro antibodies were more often detected by enzyme immunoassay. Newer antibody detection methods (immunoblot and enzyme immunoassay) are less time consuming than immunodiffusion and show good interassay sensitivity without loss of specificity. A combination of immunoblot and enzyme immunoassay yielded excellent assay sensitivity (100%) and specificity (99%) for detection of autoantibodies. C1 UNIV WISCONSIN,SCH MED,DEPT MED,CLIN IMMUNOL SERV,MADISON,WI. UNIV WISCONSIN,SCH MED,DEPT BIOSTAT,MADISON,WI. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. NR 19 TC 13 Z9 15 U1 1 U2 1 PU AMER SOC CLIN PATHOLOGISTS PI CHICAGO PA 2100 W HARRISON ST, CHICAGO, IL 60612 SN 0002-9173 J9 AM J CLIN PATHOL JI Am. J. Clin. Pathol. PD OCT PY 1997 VL 108 IS 4 BP 406 EP 410 PG 5 WC Pathology SC Pathology GA XX434 UT WOS:A1997XX43400007 PM 9322593 ER PT J AU Kramer, P Zareparsi, S Kaye, J Camicioli, R Nutt, J Litt, M Payami, H AF Kramer, P Zareparsi, S Kaye, J Camicioli, R Nutt, J Litt, M Payami, H TI Exclusion of the locus for Parkinson's disease on Chromosome 4 in three families. SO AMERICAN JOURNAL OF HUMAN GENETICS LA English DT Meeting Abstract C1 Portland Vet Affairs Med Ctr, Portland, OR USA. Oregon Hlth Sci Univ, Portland, OR 97201 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5801 S ELLIS AVENUE, CHICAGO, IL 60637 USA SN 0002-9297 J9 AM J HUM GENET JI Am. J. Hum. Genet. PD OCT PY 1997 VL 61 IS 4 SU S MA 1639 BP A281 EP A281 PG 1 WC Genetics & Heredity SC Genetics & Heredity GA YQ995 UT WOS:000071446001642 ER PT J AU Chow, MT DiSilvestro, VA Yung, CY Nawab, ZM Leehey, DJ Ing, TS AF Chow, MT DiSilvestro, VA Yung, CY Nawab, ZM Leehey, DJ Ing, TS TI Treatment of acute methanol intoxication with hemodialysis using an ethanol-enriched, bicarbonate-based dialysate SO AMERICAN JOURNAL OF KIDNEY DISEASES LA English DT Article DE methanol; bicarbonate; hemodialysis; ethanol; dialysate ID THERAPY AB A patient poisoned with methanol was successfully hemodialyzed with an ethanol-enriched, bicarbonate-based dialysate, Along with a concomitant intravenous infusion of ethanol, the ethanol-enriched dialytic procedure was able to maintain an intradialytic plasma ethanol level of 80 to 102 mg/dL. The patient recovered without any sequelae of methanol intoxication. (C) 1997 by the National Kidney Foundation, Inc. C1 US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,DEPT MED,HINES,IL 60141. LOYOLA UNIV,DEPT MED,CHICAGO,IL 60611. LOYOLA UNIV,STRITCH SCH MED,MAYWOOD,IL 60153. NR 15 TC 22 Z9 24 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0272-6386 J9 AM J KIDNEY DIS JI Am. J. Kidney Dis. PD OCT PY 1997 VL 30 IS 4 BP 568 EP 570 DI 10.1016/S0272-6386(97)90318-8 PG 3 WC Urology & Nephrology SC Urology & Nephrology GA YA577 UT WOS:A1997YA57700019 PM 9328374 ER PT J AU Abrass, CK Spicer, D Berfield, AK StJohn, PL Abrahamson, DR AF Abrass, CK Spicer, D Berfield, AK StJohn, PL Abrahamson, DR TI Diabetes induces changes in glomerular development and laminin-beta 2 (s-laminin) expression SO AMERICAN JOURNAL OF PATHOLOGY LA English DT Article ID RAT MESANGIAL CELLS; EXTRACELLULAR-MATRIX; BASEMENT-MEMBRANE; DEVELOPING KIDNEY; NEUROMUSCULAR-JUNCTION; GROWTH-FACTORS; MESSENGER-RNA; HIGH GLUCOSE; IN-VITRO; COLLAGEN AB Offspring of diabetic mothers have developmental renal abnormalities; thus, we investigated the effects of the diabetic milieu on kidney development. Four groups of host rats, including insulin-deficient and insulin-treated streptozotocin-induced diabetic rats, normal controls, and insulin-treated nondiabetic rats, were prepared. After 38 days, rats received ocular implants of E14 fetal rat kidneys. Nine days later the fetal kidney grafts were harvested for analysis of glomerular development and expression of fibronectin, laminin, laminin-beta 2, and alpha-smooth muscle actin and m170, two additional markers of mesangial maturation. The rate of glomerular maturation was delayed in grafts placed in hyperglycemic, insulin-deficient diabetic rats. These glomeruli contained few mesangial cells or matrix, and laminin-beta 2 expression was reduced as compared with controls. Mesangial expression of alpha-smooth muscle actin and m170 was not detected. In contrast, grafts placed in insulin-treated diabetic animals had increased numbers of mesangial cells and expanded mesangial matrix. The content of laminin-beta 2 and expression of m170 and alpha-smooth muscle actin were also increased in these grafts. These data show that hyperglycemia and insulin status influence laminin isoform expression and play important roles in mesangial development. C1 VET AFFAIRS PUGET SOUND HLTH CARE SYST,DEPT MED,DIV NEPHROL,SEATTLE,WA 98108. UNIV WASHINGTON,SCH MED,SEATTLE,WA. UNIV ALABAMA,DEPT CELL BIOL,BIRMINGHAM,AL 35294. FU NIDDK NIH HHS [R01 DK34972, R01 DK49771, R01 DK37891] NR 43 TC 24 Z9 24 U1 0 U2 0 PU AMER SOC INVESTIGATIVE PATHOLOGY, INC PI BALTIMORE PA 428 EAST PRESTON ST, BALTIMORE, MD 21202-3993 SN 0002-9440 J9 AM J PATHOL JI Am. J. Pathol. PD OCT PY 1997 VL 151 IS 4 BP 1131 EP 1140 PG 10 WC Pathology SC Pathology GA XZ753 UT WOS:A1997XZ75300028 PM 9327747 ER PT J AU Ullian, ME Raymond, JR Willingham, MC Paul, RV AF Ullian, ME Raymond, JR Willingham, MC Paul, RV TI Regulation of vascular angiotensin II receptors by EGF SO AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY LA English DT Article DE vascular smooth muscle cells; mitogen-activated protein kinase; G proteins ID SMOOTH-MUSCLE CELLS; GROWTH-FACTOR; PHOSPHOLIPASE-C; KINASE INHIBITORS; BINDING; RAT; ALDOSTERONE; PHOSPHORYLATION; PROTEIN; PROLIFERATION AB After vascular endothelial injury, angiotensin II (ANG II) plays a role in the resulting hypertrophic response, and expression of epidermal growth factor (EGF) is enhanced. Therefore, we tested the possibility that EGF regulates vascular ANG II action and receptor expression. Incubation of cultured aortic vascular smooth muscle cells (VSMC) with EGF (or basic fibroblast growth factor but not platelet-derived growth factor isoforms) resulted in concentration-dependent (1-50 ng/ml EGF), time-dependent (> 8 h), and reversible decreases in ANG II surface receptor density. For example, a 50% reduction was observed after exposure to 50 ng/ml EGF for 24 h. Incubation of cultured VSMC with 50 ng/ml EGF for 24 h resulted in a 77% reduction in ANG II-stimulated inositol phosphate formation. EGF not only prevented but also reversed ANG II receptor upregulation by 100 nM corticosterone. The specific tyrosine kinase inhibitor tyrphostin A48 (50 mu M) reduced EGF-stimulated thymidine incorporation and EGF-stimulated phosphorylation of mitogen-activated protein kinase but did not prevent EGF from reducing ANG II receptor density. Neither pertussis toxin (100 ng/ml) nor downregulation of protein kinase C by phorbol myristate acetate (100 nM for 24 h) prevented EGF from reducing ANG; II receptor density. In summary, EGF is a potent negative regulator of vascular ANG II surface receptor density and ANG II action by mechanisms that do not appear to include tyrosine phorphorylation, pertussis toxin-sensitive G proteins, or phorbol ester-sensitive protein kinase C. The possibility that EGF shifts the cell culture phenotype to one that exhibits reduced surface ANG II density cannot be eliminated by the present studies. C1 RALPH H JOHNSON VET AFFAIRS MED CTR, DEPT MED, DIV NEPHROL, CHARLESTON, SC 29425 USA. RALPH H JOHNSON VET AFFAIRS MED CTR, DEPT PATHOL, CHARLESTON, SC 29425 USA. RP Ullian, ME (reprint author), MED UNIV S CAROLINA, DEPT MED, DIV NEPHROL, CLIN SCI BLDG 829, 171 ASHLEY AVE, CHARLESTON, SC 29425 USA. FU NIDDK NIH HHS [DK-52448] NR 26 TC 17 Z9 17 U1 0 U2 1 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0363-6143 J9 AM J PHYSIOL-CELL PH JI Am. J. Physiol.-Cell Physiol. PD OCT PY 1997 VL 273 IS 4 BP C1241 EP C1249 PG 9 WC Cell Biology; Physiology SC Cell Biology; Physiology GA YB153 UT WOS:A1997YB15300016 PM 9357768 ER PT J AU Patrick, DL Pearlman, RA Starks, HE Cain, KC Cole, WG Uhlmann, RF AF Patrick, DL Pearlman, RA Starks, HE Cain, KC Cole, WG Uhlmann, RF TI Validation of preferences for life-sustaining treatment: Implications for advance care planning SO ANNALS OF INTERNAL MEDICINE LA English DT Article DE advance directives; life support care; health status; treatment outcomes ID STATES WORSE; DIRECTIVES; ATTITUDES; STABILITY; CHOICES; QUALITY; VALUES; DEATH AB Background: Treatment preferences established before life-threatening illness occurs may differ from actual decisions because of changes in preferences or poor understanding of the link between prospective preferences and outcomes. Objective: To evaluate the validity of prospective treatment preferences by examining their concordance with ratings of health states. Design: Survey of seven cohorts of persons with diverse health status. Home-and hospital-based interviews were conducted at baseline and at 6, 18, and 30 months. Setting: The greater Seattle area. Participants: Younger and older well adults; persons with chronic conditions, terminal cancer, or AIDS; stroke survivors; and nursing home residents. Measurements: Concordance between six treatment preferences and five health state ratings (on a seven-point scale) was assessed by using logistic regression to measure the increase in odds of treatment refusal for each one-point change in health state ratings. Preferences were considered concordant if treatments were refused in health states rated as worse than death and were accepted in health states rated as better than death. Reasons for discordance were elicited at the final interview. Results: The probability of refusal of prospective treatment was strongly related to health state ratings. Odds ratios ranged from 1.7 to 1.9 (P < 0.001) for every treatment. When patients were shown their discordant preferences, they had a coherent explanation or changed their health state rating or treatment preference to make the two concordant. Conclusions: Prospective life-sustaining treatment preferences show high convergent validity. For most persons, treatment preferences are grounded in a consistent belief system. Concordance and discordance between treatment preferences and health state ratings offer clinicians the opportunity to explore patients' Values and reasoning. C1 VET AFFAIRS PUGET SOUND HLTH CARE SYST,SEATTLE,WA 98108. UNIV WASHINGTON,DEPT MED,SEATTLE,WA 98195. RP Patrick, DL (reprint author), UNIV WASHINGTON,DEPT HLTH SERV,BOX 357660,SEATTLE,WA 98195, USA. FU AHRQ HHS [HS06343] NR 25 TC 77 Z9 81 U1 1 U2 6 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD OCT 1 PY 1997 VL 127 IS 7 BP 509 EP & PG 10 WC Medicine, General & Internal SC General & Internal Medicine GA XY733 UT WOS:A1997XY73300002 PM 9313018 ER PT J AU Zareparsi, S Kaye, J Camicioli, R Grimslid, H Oken, B Litt, M Nutt, J Bird, T Schellenberg, G Payami, H AF Zareparsi, S Kaye, J Camicioli, R Grimslid, H Oken, B Litt, M Nutt, J Bird, T Schellenberg, G Payami, H TI Modulation of the age at onset of Parkinson's disease by apolipoprotein E genotypes SO ANNALS OF NEUROLOGY LA English DT Article ID ALZHEIMERS-DISEASE; EPSILON-4 ALLELE; DEMENTIA; FREQUENCY; GENE; INDIVIDUALS; RISK AB Parkinson's disease (PD) patients often develop dementia, and Alzheimer's disease (AD) patients frequently develop parkinsonian signs. The apolipoprotein E epsilon 4 allele is associated with increased risk and earlier onset of AD. We studied 137 unrelated white PD patients. Those with epsilon 4 had the earliest onset (52.7 +/- 9.8 years), epsilon 3/epsilon 3 patients had an intermediate onset (56.1 +/- 11.1 years), and those with epsilon 2 had the latest onset (59.1 +/- 13.4 years). The age at onset distribution for epsilon 4/epsilon- was significantly earlier than for epsilon 3/epsilon 3 and epsilon 2/epsilon 3. These preliminary results suggest that apolipoprotein E genotypes modulate the age at onset of PD. C1 OREGON HLTH SCI UNIV,DEPT NEUROL,PORTLAND,OR 97201. OREGON HLTH SCI UNIV,DEPT BIOCHEM & MOL BIOL,PORTLAND,OR 97201. PORTLAND VET AFFAIRS MED CTR,PORTLAND,OR. UNIV WASHINGTON,DEPT NEUROL,SEATTLE,WA 98195. UNIV WASHINGTON,DEPT MED,SEATTLE,WA 98195. UNIV WASHINGTON,DEPT PHARMACOL,SEATTLE,WA 98195. RP Zareparsi, S (reprint author), OREGON HLTH SCI UNIV,CDW2,DEPT MOL & MED GENET,745 SW GAINES RD,PORTLAND,OR 97201, USA. OI Kaye, Jeffrey/0000-0002-9971-3478; Camicioli, Richard/0000-0003-2977-8660 FU NIA NIH HHS [AG05136, AG0817] NR 24 TC 47 Z9 47 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0364-5134 J9 ANN NEUROL JI Ann. Neurol. PD OCT PY 1997 VL 42 IS 4 BP 655 EP 658 DI 10.1002/ana.410420417 PG 4 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA YA463 UT WOS:A1997YA46300016 PM 9382478 ER PT J AU Mironova, M Virella, G LopesVirella, MF AF Mironova, M Virella, G LopesVirella, MF TI Isolation and characterization of human antioxidized LDL autoantibodies (vol 16, pg 222, 1996) SO ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY LA English DT Correction, Addition C1 MED UNIV S CAROLINA,DEPT MED,DIV ENDOCRINOL DIABET & MED GENET,CHARLESTON,SC 29425. RALPH H JOHNSON VA MED CTR,CHARLESTON,SC. RP Mironova, M (reprint author), MED UNIV S CAROLINA,DEPT MICROBIOL & IMMUNOL,CHARLESTON,SC 29425, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 1079-5642 J9 ARTERIOSCL THROM VAS JI Arterioscler. Thromb. Vasc. Biol. PD OCT PY 1997 VL 17 IS 10 BP 2306 EP 2306 PG 1 WC Hematology; Peripheral Vascular Disease SC Hematology; Cardiovascular System & Cardiology GA YE282 UT WOS:A1997YE28200061 ER PT J AU Escary, JL Choy, HA Reue, K Schotz, MC AF Escary, JL Choy, HA Reue, K Schotz, MC TI Hormone-sensitive lipase overexpression increases cholesteryl-ester hydrolysis in macrophage foam cells SO ATHEROSCLEROSIS LA English DT Meeting Abstract C1 W LOS ANGELES VA MED CTR,LIPID RES LAB,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90024. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0021-9150 J9 ATHEROSCLEROSIS JI Atherosclerosis PD OCT PY 1997 VL 134 IS 1-2 SI SI BP 230 EP 230 DI 10.1016/S0021-9150(97)89225-8 PG 1 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA XY106 UT WOS:A1997XY10601057 ER PT J AU MarquesMagallanes, JA Koyal, SN Cooper, CB Kleerup, EC Tashkin, DP AF MarquesMagallanes, JA Koyal, SN Cooper, CB Kleerup, EC Tashkin, DP TI Impact of habitual cocaine smoking on the physiologic response to maximum exercise SO CHEST LA English DT Article DE cardiovascular response; cocaine smoking; crack; gas exchange; maximum exercise; ventilatory response ID GAS-EXCHANGE; ENDURANCE; RATS; CATECHOLAMINE; WITHDRAWAL; CRACK; USERS AB Background: Habitual smoking of alkaloidal cocaine (crack) has been reported to be associated with a number of cardiopulmonary complications that may not be clinically obvious but could potentially interfere with normal physiologic responses to exercise and thus impair maximum exercise performance. Study objective: To evaluate the impact of regular use of cocaine on maximum exercise. Design: Observational study in crack users and age- and gender-matched control subjects. Subjects: Thirty-five habitual cocaine smokers (21 male and 14 female) and 29 age-matched sedentary control nonsmokers of cocaine (15 male and 14 female), all of whom were in good general health. Methods: In these subjects, we compared physiologic responses to symptom-limited, incremental maximal exercise performed on a cycle ergometer using a ramp protocol. Comparisons were made for men and women separately. Results: For both men and women, long-term cocaine smokers had a reduced aerobic capacity (maximum oxygen consumption) compared with control nonsmokers but did not show evidence of ventilatory limitation, reduced gas exchange threshold, increased physiologic dead space, or gas exchange abnormality at maximum exercise compared with the healthy control subjects. Although cocaine smokers had reduced maximum heart rates compared with control subjects, the relationship between submaximal heart rate and oxygen uptake was normal, indicating a normal cardiovascular response pattern. However, effort perception was similar between the two groups despite the difference in heart rate at maximum exercise, suggesting the possibility of perceptual dysfunction for effort. Differences in aerobic capacity between the crack users and nonusers could not be explained by differences in physical fitness or altered perception of dyspnea. Conclusion: In the subjects we studied, long-term cocaine smoking was associated with reduced maximum exercise performance, probably due to poor motivation or altered effort perception. No other identifiable physiologic abnormality appeared to limit exercise in the habitual crack users. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90095. UNIV CALIF LOS ANGELES,SCH MED,DEPT PHYSIOL,LOS ANGELES,CA 90095. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. FU NIDA NIH HHS [R01 DA08254, R01 DA008254] NR 29 TC 11 Z9 11 U1 0 U2 1 PU AMER COLL CHEST PHYSICIANS PI NORTHBROOK PA 3300 DUNDEE ROAD, NORTHBROOK, IL 60062-2348 SN 0012-3692 J9 CHEST JI Chest PD OCT PY 1997 VL 112 IS 4 BP 1008 EP 1016 DI 10.1378/chest.112.4.1008 PG 9 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA YA351 UT WOS:A1997YA35100025 PM 9377910 ER PT J AU Mironova, M Virella, G VirellaLowell, I LopesVirella, MF AF Mironova, M Virella, G VirellaLowell, I LopesVirella, MF TI Anti-modified LDL antibodies and LDL-containing immune complexes in IDDM patients and healthy controls SO CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article ID LOW-DENSITY-LIPOPROTEIN; MONOCYTE-DERIVED MACROPHAGES; FOAM CELL-FORMATION; HUMAN RED-CELLS; DIABETES-MELLITUS; AUTOANTIBODIES; ATHEROSCLEROSIS; PROTEINS; PLASMA; GLYCOSYLATION AB Antibodies to oxidized LDL (ox-LDL) and LDL containing immune complexes (LDL-IC) have been reported to be associated with the presence or progression of arteriosclerosis. We screened for anti-modified LDL antibodies and isolated soluble IC by precipitation with 3.5% (w/v) polyethylene glycol (PEG) 6000 in two groups. The patient group was constituted by 16 insulin-dependent diabetes mellitus subjects free of macrovascular complications. The control group was constituted by 16 healthy, age-, gender-, race-, and body mass index-matched nondiabetic subjects. We detected anti-ox-LDL antibodies and anti-malondialdehyde-modified LDL antibodies with similar levels in patients and controls, while the levels of anti-glycated LDL antibodies were very low, but slightly higher in diabetics than in healthy controls. Isolated LDL-IC were adsorbed to red blood cells (RBC) and incubated with human macrophages for 18 hr at 37 degrees C. Under those experimental conditions, RBC-adsorbed IC are taken up by macrophages but the RBC remain intact and are not ingested. Slightly higher levels of cholesteryl ester (CE) accumulation were measured in macrophages incubated with RBC to which we adsorbed IC isolated from diabetics (15.4 +/- 2.5 mu g/mg of protein, mean +/- SEM) than in macrophages incubated with IC isolated from controls (12.5 +/- 1.6 mu g/mg of protein, mean +/- SEM), but the difference did not reach statistical significance. PEG-precipitable IC isolated from both normal and diabetic subjects led, in some instances, to the transformation of macrophages into foam cells. Significant correlations were observed between CE accumulation and the content of apo B (P < 0.0001), total cholesterol (P = 0.0004), IgG (P = 0.015), and IgA (P = 0.015) in the isolated IC. The correlation between CE accumulation and the content of apo B in isolated IC was stronger in diabetics than in the control group (r = 0.759 vs r = 0.500). Fractionation of isolated IC in immobilized protein A/G yielded immunoglobulin-rich fractions which contained cholesterol and IgG anti-ox-LDL antibodies. The cholesterol content of these fractions was significantly correlated (P = 0.001) with CE accumulation. In conclusion, both diabetics and normal individuals have circulating IC whose atherogenic potential appears to be related to the presence of LDL and antibodies of the IgG and IgA isotypes. (C) 1997 Academic Press. C1 MED UNIV S CAROLINA,DEPT MICROBIOL & IMMUNOL,CHARLESTON,SC 29401. MED UNIV S CAROLINA,RALPH H JOHNSON DEPT VET AFFAIRS MED CTR,CHARLESTON,SC 29401. RP Mironova, M (reprint author), MED UNIV S CAROLINA,DEPT MED,DIV ENDOCRINOL METAB & MED GENET,CHARLESTON,SC 29401, USA. FU NHLBI NIH HHS [HL46815] NR 37 TC 40 Z9 40 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0090-1229 J9 CLIN IMMUNOL IMMUNOP JI Clin. Immunol. Immunopathol. PD OCT PY 1997 VL 85 IS 1 BP 73 EP 82 DI 10.1006/clin.1997.4404 PG 10 WC Immunology; Pathology SC Immunology; Pathology GA YA706 UT WOS:A1997YA70600010 PM 9325072 ER PT J AU Fichtner, CG Poddig, BE deVito, RA AF Fichtner, CG Poddig, BE deVito, RA TI Post-traumatic stress disorder - Pathophysiological aspects and pharmacological approaches to treatment SO CNS DRUGS LA English DT Review ID VIETNAM COMBAT VETERANS; GLUCOCORTICOID RECEPTOR NUMBER; CONCURRENT PSYCHIATRIC-ILLNESS; PLATELET ADENYLATE-CYCLASE; PLASMA BETA-ENDORPHIN; MONOAMINE-OXIDASE; WAR NEUROSIS; PSYCHOPHARMACOLOGICAL TREATMENT; PSYCHOPHYSIOLOGICAL RESPONSES; CATECHOLAMINE EXCRETION AB Post-traumatic stress disorder (PTSD) is a syndrome of psychophysiological sequelae occurring in the aftermath of severe emotional trauma. Phenomenologically, symptoms occur in 3 clusters: re-experiencing, avoidance and hyperarousal. Other psychiatric disorders such as depression, panic disorder and substance abuse frequently co-occur with PTSD. Treatment strategies for PTSD are often multimodal and attempt to integrate biological, behavioural, cognitive, psychodynamic and social formulations. Pharmacotherapy must target specific symptoms in all 3 clusters, and address the presence of comorbid psychiatric disorders. Psychotropic medication can facilitate psychotherapeutic work, or serve as the primary modality in a biologically based approach to treatment. Specific medications used in the treatment of PTSD span a broad spectrum of pharmacological agents. The most empirically studied agents, tricyclic antidepressants and monoamine oxidase inhibitors, demonstrate a definite but limited impact on specific PTSD symptoms. For the patient who presents in an acute symptomatic state, benzodiazepine anxiolytics may be appropriate. For longer term treatment, selective serotonin (5-hydroxytryptamine; 5-HT) reuptake inhibitors (SSRIs), particularly fluoxetine, appear to have broader spectrum therapeutic effects on specific PTSD symptom clusters. Both antidepressants and anxiolytics have, in some studies, shown beneficial effects on depressive symptoms and anxiety, without measurable impact on the symptoms more specific to PTSD. Maintenance pharmacotherapy frequently requires a multidrug regimen; an SSRI may serve as the primary agent, with adjunctive agents targeting residual hyperarousal and re-experiencing symptoms. Benzodiazepines, sedating tricyclic or antihistamine compounds, and the noradrenergic drugs clonidine and propranolol are used in this way. Buspirone, a serotonergic anxiolytic, may serve a similar role for some patients, and cyproheptadine in particular appears to be helpful for nightmares. The antikindling agents carbamazepine and valproic acid (sodium valproate) have also been reported to be beneficial, and may provide an alternative for patients with persistent hyper-reactivity and explosiveness that have not responded to other treatments. C1 FINCH UNIV HLTH SCI CHICAGO MED SCH, DEPT PSYCHIAT & BEHAV SCI, CHICAGO, IL USA. US DEPT VET AFFAIRS, VET AFFAIRS EDWARD HINES JR HOSP, SERV PHARM, HINES, IL 60141 USA. LOYOLA UNIV, STRITCH SCH MED, DEPT PSYCHIAT, MAYWOOD, IL 60153 USA. RP Fichtner, CG (reprint author), ILLINOIS DEPT HUMAN SERV, DIV CLIN SCI, 100 W RANDOLPH ST, SUITE 6-400, CHICAGO, IL 60601 USA. NR 239 TC 17 Z9 17 U1 2 U2 4 PU ADIS INT LTD PI AUCKLAND PA 41 CENTORIAN DR, PRIVATE BAG 65901, MAIRANGI BAY, AUCKLAND 1311, NEW ZEALAND SN 1172-7047 J9 CNS DRUGS JI CNS Drugs PD OCT PY 1997 VL 8 IS 4 BP 293 EP 322 DI 10.2165/00023210-199708040-00004 PG 30 WC Clinical Neurology; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA YA335 UT WOS:A1997YA33500004 ER PT J AU Goldstein, O Asher, C Cragoe, E Kleyman, TR Garty, H AF Goldstein, O Asher, C Cragoe, E Kleyman, TR Garty, H TI An aldosterone regulated chicken intestine protein with high affinity to amiloride SO COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY LA English DT Article; Proceedings Paper CT 4th Abildgaard Symposium on Intestinal NaCl and SCFA Transport CY AUG 19-22, 1995 CL ROYAL VET & AGR UNIV, COPENHAGEN, DENMARK HO ROYAL VET & AGR UNIV DE amiloride-binding protein; epithelial transport; ion transport; Na+ channel; NaCl intake ID EPITHELIAL SODIUM-CHANNEL; SENSITIVE NA+ CHANNEL; MEMBRANE-VESICLES; BINDING-PROTEIN; TIGHT EPITHELIA; ION-TRANSPORT; H-3 PHENAMIL; EXPRESSION; CLONING; ANALOGS AB The pattern of chicken intestine amiloride-binding proteins was determined using the photoreactive amiloride analogue 2'-methoxy-5'-nitrobenzamil (NMBA) and a polyclonal anti-amiloride antibody. At 10(-7)M, NMBA inhibits similar to 62% of the Na+ channel activity. At this concentration the amiloride analogue labels a number of membrane proteins, and in particular a 40-45 kDa polypeptide denoted ABP40. Incorporation of NMBA into ABP40 could be prevented by a 100-fold excess of benzamil, but not by a 1000-fold excess of 5-(N-ethyl-N-isopropyl)-amiloride. Labeling of ABP40 was intense in membranes derived from salt-deprived chickens and similar to 5-fold weaker in membranes from salt-repleted animals. Because of its small size, ABP40 is not likely to be an avian Na+ channel subunit, yet this amiloride-binding protein could be involved in the response to aldosterone. (C) 1997 Elsevier Science Inc. C1 WEIZMANN INST SCI, DEPT MEMBRANE RES & BIOPHYS, IL-76100 REHOVOT, ISRAEL. UNIV PENN, DEPT PHYSIOL & MED, PHILADELPHIA, PA 19104 USA. VET AFFAIRS MED CTR, PHILADELPHIA, PA 19104 USA. NR 45 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1095-6433 EI 1531-4332 J9 COMP BIOCHEM PHYS A JI Comp. Biochem. Physiol. A-Mol. Integr. Physiol. PD OCT PY 1997 VL 118 IS 2 BP 201 EP 208 DI 10.1016/S0300-9629(97)00067-4 PG 8 WC Biochemistry & Molecular Biology; Physiology; Zoology SC Biochemistry & Molecular Biology; Physiology; Zoology GA XY783 UT WOS:A1997XY78300003 PM 9366044 ER PT J AU Goldstein, G AF Goldstein, G TI Clinical neuropsychology of alcoholism - Knight,RG, Longmore,BE SO CONTEMPORARY PSYCHOLOGY LA English DT Book Review RP Goldstein, G (reprint author), VET AFFAIRS PITTSBURGH HEALTHCARE SYST,PITTSBURGH,PA, USA. NR 4 TC 0 Z9 0 U1 0 U2 3 PU AMER PSYCHOLOGICAL ASSOC PI WASHINGTON PA 750 FIRST ST NE, WASHINGTON, DC 20002-4242 SN 0010-7549 J9 CONTEMP PSYCHOL JI Comtemp. Psychol. PD OCT PY 1997 VL 42 IS 10 BP 918 EP 919 PG 2 WC Psychology, Multidisciplinary SC Psychology GA XZ236 UT WOS:A1997XZ23600028 ER PT J AU Salinsky, MC AF Salinsky, MC TI A practical analysis of computer based seizure detection during continuous video-EEG monitoring SO ELECTROENCEPHALOGRAPHY AND CLINICAL NEUROPHYSIOLOGY LA English DT Article DE electroencephalography; telemetry; epilepsy; seizure; computer aided diagnosis ID EPILEPTIC SEIZURES AB Computer based seizure detection (CSD) systems improve the efficiency of CCTV-EEG monitoring by capturing epileptic seizures which would have otherwise been missed. We prospectively evaluated the yield of a commercial CSD system in 83 consecutive CCTV-EEG admissions. All seizures were coded as to the method of detection. The percentage of seizures detected only by CSD was calculated for each patient, and the impact on length of hospital stay was estimated. Overall, 33% of epileptic seizures were signaled by the patient, 45% were directly observed by family or medical personnel, and 22% were captured only by CSD. Forty admissions (48%) had at least one seizure captured only by CSD. The majority of these events were clinical and electrographic seizures (73%) and the remainder were purely electrographic. Five admissions concluded with all seizures captured only by CSD. We estimated an average saving of 1.3 hospital days per admission, based on the percentage of seizures captured only by CSD. (C) 1997 Elsevier Science Ireland Ltd. C1 PORTLAND VET AFFAIRS MED CTR,PORTLAND,OR. RP Salinsky, MC (reprint author), OREGON HLTH SCI UNIV,EPILEPSY CTR CDW3,3181 SW SAM JACKSON PK RD,PORTLAND,OR 97201, USA. NR 11 TC 19 Z9 19 U1 0 U2 2 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0013-4694 J9 ELECTROEN CLIN NEURO JI Electroencephalogr. Clin. Neurophysiol. PD OCT PY 1997 VL 103 IS 4 BP 445 EP 449 DI 10.1016/S0013-4694(97)00025-4 PG 5 WC Engineering, Biomedical; Clinical Neurology SC Engineering; Neurosciences & Neurology GA YE154 UT WOS:A1997YE15400005 PM 9368489 ER PT J AU Royall, DR Cordes, J Polk, M AF Royall, DR Cordes, J Polk, M TI Executive control and the comprehension of medical information by elderly retirees SO EXPERIMENTAL AGING RESEARCH LA English DT Article ID MINI-MENTAL-STATE; ALZHEIMERS-DISEASE; COMPETENCE; IMPAIRMENT; BEHAVIOR AB This study examined the independent contributions of executive control function, general cognition, age, education, and medication usage to the comprehension of medical information. Randomly selected elderly retirees (N = 105) more than 70 years of age completed the Executive Interview (EXIT25), the Mini-Mental State Exam (MMSE), and the Hopkins Competency Assessment Test (HCAT), Cognitive measures were stronger predictors of HCAT scores than age, education, or number of prescribed medications. A discriminant model bused on EXIT25 and MMSE scores correctly classified 91% of subjects relative to their HCAT scores. It was concluded that executive impairment is strongly associated with impaired comprehension of medical information, As many as 88% of probable Alzheimer's disease patients, 69% of institutionalized elderly retirees, and 49% of noninstitutionalized retirees may be impaired in their ability to comprehend medical information, even when it has been presented well below their educational level. C1 UNIV TEXAS,HLTH SCI CTR,DIV CLIN PHARMACOL,SAN ANTONIO,TX. RP Royall, DR (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT PSYCHIAT,S TEXAS VET HEALTHCARE SYST,SAN ANTONIO,TX 78284, USA. NR 31 TC 59 Z9 59 U1 0 U2 0 PU TAYLOR & FRANCIS PI BRISTOL PA 1900 FROST ROAD, SUITE 101, BRISTOL, PA 19007-1598 SN 0361-073X J9 EXP AGING RES JI Exp. Aging Res. PD OCT-DEC PY 1997 VL 23 IS 4 BP 301 EP 313 DI 10.1080/03610739708254033 PG 13 WC Geriatrics & Gerontology; Psychology SC Geriatrics & Gerontology; Psychology GA YC446 UT WOS:A1997YC44600001 PM 9352289 ER PT J AU Cornford, EM Varesi, JB Hyman, S Damian, RT Raleigh, MJ AF Cornford, EM Varesi, JB Hyman, S Damian, RT Raleigh, MJ TI Mitochondrial content of choroid plexus epithelium SO EXPERIMENTAL BRAIN RESEARCH LA English DT Article DE blood-CSF barrier mitochondrial volume; digital electron microscopy; cuboidal epithelium; rat; mouse; rabbit; monkey ID BLOOD-BRAIN-BARRIER; ENDOTHELIAL-CELL MITOCHONDRIA; MORPHOMETRIC ANALYSIS; GLUCOSE-TRANSPORTER; RAT; ULTRASTRUCTURE; NUMBER; LOCALIZATION; MICROVESSELS; MEMBRANES AB The objective of the present study was to examine the apparent work capacity of one of the two separate membrane systems (the blood-cerebrospinal fluid barrier) that isolate the mammalian brain extracellular fluid (and cerebrospinal fluid, CSF) from plasma. Digitized analyses of electron-microscopic images provided estimates of mitochondrial volumes, which were expressed as a percentage of the cell cytoplasm. We recorded a high mitochondrial content of 12-15% in the cuboidal epithelium of primate choroid plexus, which was consistent in vervet, rhesus, and squirrel monkeys, as well as in baboons. Similarly high mitochondrial contents were observed in the rabbit, rat, and mouse choroid plexus. It has been postulated that the high mitochondrial content of brain endothelium is associated with maintaining the ionic gradients within the central nervous system. We observed that the mitochondrial content of the choroid plexus (where CSF is produced) was slightly higher than in (prior measurements of) the blood-brain barrier (BBB). In addition, surface areas at the apical borders of the choroid plexus epithelia (where the Na(+)K(+)ATPase activity has been localized) were increased 7- to 13-fold over the basal borders, in the primate species examined. The observation of high mitochondrial volumes in choroid plexus cells is consistent with the suggestion that increased mitochondrial densities seen in choroidal epithelia and BBB capillaries provide a metabolic work capability for both secretory activities and maintaining ionic gradients across blood-CSF barriers. C1 W LOS ANGELES VET AFFAIRS MED CTR,NEUROL SERV,SW REG VA EPILEPSY CTR W12B,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,DEPT PSYCHIAT,LOS ANGELES,CA 90095. UNIV CALIF SANTA BARBARA,SCH ENGN,SANTA BARBARA,CA 93106. UNIV CALIF LOS ANGELES,SCH MED,INST BRAIN RES,LOS ANGELES,CA 90095. UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROL,LOS ANGELES,CA 90095. RP Cornford, EM (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,RES SERV,SW REG VA EPILEPSY CTR W12B,LOS ANGELES,CA 90073, USA. FU NINDS NIH HHS [NS 25554] NR 28 TC 27 Z9 28 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0014-4819 J9 EXP BRAIN RES JI Exp. Brain Res. PD OCT PY 1997 VL 116 IS 3 BP 399 EP 405 DI 10.1007/PL00005768 PG 7 WC Neurosciences SC Neurosciences & Neurology GA YC086 UT WOS:A1997YC08600003 PM 9372289 ER PT J AU Gorry, MC Gabbaizedeh, D Furey, W Gates, LK Preston, RA Aston, CE Zhang, YZ Ulrich, C Ehrlich, GD Whitcomb, DC AF Gorry, MC Gabbaizedeh, D Furey, W Gates, LK Preston, RA Aston, CE Zhang, YZ Ulrich, C Ehrlich, GD Whitcomb, DC TI Mutations in the cationic trypsinogen gene are associated with recurrent acute and chronic pancreatitis SO GASTROENTEROLOGY LA English DT Article ID HEREDITARY PANCREATITIS; LINKAGE ANALYSIS; DNA-SEQUENCE; LONG ARM; MAPS; SPHINCTER; ODDI AB Background & Aims: We recently identified a single R117H mutation in the cationic trypsinogen gene in several kindreds with an inherited form of acute and chronic pancreatitis (HP1), providing strong evidence that trypsin plays a central role in premature zymogen activation and pancreatitis, However, not all families studied have this mutation, The aim of this study was to determine the disease-causing mutation in kindreds with hereditary pancreatitis that lack the previously identified mutation. Methods: Clinical features of the HP1 kindreds were compared with those of the new kindreds (HP2), and genetic linkage analysis, screening for mutations through DNA sequencing, and screening an unaffected population were performed. Results: The onset of symptoms was delayed and hospitalizations were fewer in HP2 compared with HP1(P < 0.05). Linkage of the disease gene to chromosome 7q35 was established (logarithm of the odds, 3.73), Mutational screening identified a single A to T mutation resulting in an asparagine to isoleucine transition mutation at position 21 (N21I) in cationic trypsinogen. The mutation was absent in 94 unrelated individuals, representing 188 unique chromosomes. Conclusions: The identification of a second mutation in the cationic trypsinogen gene (HP2) suggests a dominant role of trypsin in premature protease activation-mediated forms of acute pancreatitis. The pathogenesis of hereditary pancreatitis also suggests that chronic pancreatitis may result from recurrent acute pancreatitis. C1 UNIV PITTSBURGH,DEPT MED,DIV GASTROENTEROL & HEPATOL,PITTSBURGH,PA 15261. UNIV PITTSBURGH,DEPT CRYSTALLOG,PITTSBURGH,PA 15261. UNIV PITTSBURGH,DEPT PATHOL,PITTSBURGH,PA 15261. UNIV PITTSBURGH,DEPT HUMAN GENET,PITTSBURGH,PA 15261. UNIV PITTSBURGH,DEPT CELL BIOL & PHYSIOL,PITTSBURGH,PA 15261. VET AFFAIRS PITTSBURGH HLTH CARE SYST,PITTSBURGH,PA. CHILDRENS HOSP PITTSBURGH,DEPT PEDIAT OTOLARYNGOL,PITTSBURGH,PA 15213. UNIV KENTUCKY,DEPT MED,LEXINGTON,KY 40506. UNIV CINCINNATI,DEPT MED,CINCINNATI,OH 45221. FU NIDCD NIH HHS [R01 DC02148]; NIDDK NIH HHS [R01 DK50236, R03 DK51954] NR 35 TC 312 Z9 315 U1 1 U2 7 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD OCT PY 1997 VL 113 IS 4 BP 1063 EP 1068 DI 10.1053/gast.1997.v113.pm9322498 PG 6 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA YB027 UT WOS:A1997YB02700007 PM 9322498 ER PT J AU Naliboff, BD Munakata, J Fullerton, S Gracely, RH Kodner, A Harraf, F Mayer, EA AF Naliboff, BD Munakata, J Fullerton, S Gracely, RH Kodner, A Harraf, F Mayer, EA TI Evidence for two distinct perceptual alterations in irritable bowel syndrome SO GUT LA English DT Article DE irritable bowel syndrome; visceral hypersensitivity; pain threshold ID RECTAL SENSITIVITY; DISTENSION; MOTILITY; PAIN AB Background and aims-Visceral hyperalgesia has been implicated as a factor contributing to symptom generation in irritable bowel syndrome (IBS). However, previous studies using intestinal balloon distension have used psychophysical procedures which do not provide adequate and unbiased measures of visceral sensitivity. Methods-Three psychophysical tasks were examined in 45 patients with IBS (positive Rome criteria) and 14 controls using rectal balloon distension with a computerised distension device. Discomfort threshold and tolerance were assessed during an ascending series of phasic pressure stimuli and during an interactive threshold tracking procedure. In addition, stimulus response functions were generated from intensity and unpleasantness ratings of the rectal distensions. Results-Discomfort threshold and tolerance for the ascending stimuli were significantly lower for the patients with IBS compared with the controls. In contrast, discomfort thresholds during the tracking procedure and stimulus response curves for the ascending series were not different between the groups. A factor analysis of the psychophysical data was consistent with the presence of two distinct and unrelated perceptual alterations related to rectal distension: hypervigilance for visceral stimuli, manifested as lowered response criteria for using the descriptor ''discomfort''; and rectal hypersensitivity, manifested as a lower discomfort threshold and left shift of the stimulus response curves. Conclusions-Patients with IBS as a group have a greater propensity to label visceral sensations negatively and show a lower tolerance for rectal balloon distension. A subgroup of patients also have baseline rectal hypersensitivity, assessed by unbiased measures of discomfort threshold and stimulus intensity judgements. C1 UNIV CALIF LOS ANGELES,LOS ANGELES,CA 90073. NIDR,NAB,DEPT MED,NIH,BETHESDA,MD 20892. NIDR,NAB,DEPT PHYSIOL,NIH,BETHESDA,MD 20892. NIDR,NAB,DEPT PSYCHOL,NIH,BETHESDA,MD 20892. NIDR,NAB,NEUROPATH PAIN & PAIN ASSESSMENT SECT,NIH,BETHESDA,MD 20892. RP Naliboff, BD (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,CURE DIGEST DIS RES CTR,NEUROENTER DIS PROGRAM,LOS ANGELES,CA 90073, USA. FU NIDDK NIH HHS [DK48351] NR 32 TC 279 Z9 282 U1 0 U2 0 PU BRITISH MED JOURNAL PUBL GROUP PI LONDON PA BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON, ENGLAND WC1H 9JR SN 0017-5749 J9 GUT JI Gut PD OCT PY 1997 VL 41 IS 4 BP 505 EP 512 PG 8 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA YB236 UT WOS:A1997YB23600018 PM 9391250 ER PT J AU Zaman, A Hapke, R Flora, K Rosen, H Benner, K AF Zaman, A Hapke, R Flora, K Rosen, H Benner, K TI Factors predicting the presence of esophageal and gastric varices in patients with advanced liver disease. SO HEPATOLOGY LA English DT Meeting Abstract C1 OREGON HLTH SCI UNIV,PORTLAND,OR 97201. PORTLAND VA MED CTR,PORTLAND,OR. NR 0 TC 2 Z9 2 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0270-9139 J9 HEPATOLOGY JI Hepatology PD OCT PY 1997 VL 26 IS 4 SU S BP 29 EP 29 PN 2 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA XY874 UT WOS:A1997XY87400029 ER PT J AU Raaphorst, FM Raman, CS Tami, J Fischbach, M Sanz, I AF Raaphorst, FM Raman, CS Tami, J Fischbach, M Sanz, I TI Human Ig heavy chain CDR3 regions in adult bone marrow pre-B cells display an adult phenotype of diversity: evidence for structural selection of D-H amino acid sequences SO INTERNATIONAL IMMUNOLOGY LA English DT Article DE antigen receptor; B lymphocyte; repertoire; VDJ rearrangement ID COMPLEMENTARITY-DETERMINING REGIONS; ANTIBODY-ANTIGEN COMPLEXES; PREFERENTIAL UTILIZATION; V(D)J RECOMBINATION; 3-DIMENSIONAL STRUCTURE; HYPERVARIABLE REGIONS; V(H) SEGMENTS; GENE SEGMENTS; FETAL LIVER; REPERTOIRE AB Ig repertoires generated at various developmental stages differ markedly in diversity. It is well documented that Ig H chain genes in human fetal liver are limited with regard to N-regional diversity and use of diversity elements. It is unclear whether these characteristics persist in pre-B cell H chain genes of adult bone marrow. Using Ig H chain CDR3 fingerprinting and sequence analysis, we analyzed the diversity of Ig H chain third complementarity determining regions (HCDR3) in adult bone marrow pre-B and mature a lymphocytes. pre-B cell HCDR3 sequences exhibited adult characteristics with respect to HCDR3 size, distribution of N regions and usage of diversity elements. This suggested that pre-B cells in adults are distinct from fetal a cell precursors with regard to Ig H chain diversification mechanisms. At the DNA sequence level, HCDR3 diversity in mature a cells was similar to that in pre-B cells, pre-B HCDR3s, however, frequently contained a consecutive stretch of hydrophobic amino acids, which were rare in mature a cells. We propose that highly hydrophobic pre-B HCDR3s may be negatively selected on the basis of structural limitations imposed by the antigen binding site. At the same time, usage of hydrophilic HCDR3 sequences (thought to support HCDR3 loop formation) may be promoted by positive selection. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT BIOCHEM,SAN ANTONIO,TX 78284. S TEXAS VET HLTH CARE SYST,AUDIE L MURPHY DIV,SAN ANTONIO,TX 78284. OI Raman, C. S./0000-0002-1036-3193 NR 64 TC 47 Z9 47 U1 0 U2 1 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD, ENGLAND OX2 6DP SN 0953-8178 J9 INT IMMUNOL JI Int. Immunol. PD OCT PY 1997 VL 9 IS 10 BP 1503 EP 1515 DI 10.1093/intimm/9.10.1503 PG 13 WC Immunology SC Immunology GA YD178 UT WOS:A1997YD17800011 PM 9352355 ER PT J AU Bradley, KA AF Bradley, KA TI Brief physician advice for problem drinkers SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Letter RP Bradley, KA (reprint author), VET AFFAIRS PUGET SOUND HLTH CARE SYST,SEATTLE DIV,SEATTLE,WA, USA. NR 1 TC 3 Z9 3 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD OCT 1 PY 1997 VL 278 IS 13 BP 1059 EP 1059 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA XX303 UT WOS:A1997XX30300013 PM 9315754 ER PT J AU Asch, DA Jedrziewski, MK Christakis, NA AF Asch, DA Jedrziewski, MK Christakis, NA TI Response rates to mail surveys published in medical journals SO JOURNAL OF CLINICAL EPIDEMIOLOGY LA English DT Article DE cost-effectiveness; data collection; epidemiology; financial incentives; health services research; mailed survey; research design; response bias; survey methods ID INCENTIVES; NONRESPONDENTS; METAANALYSIS; STRATEGIES; BEHAVIOR; BIAS AB Objective. The purpose of this study was to characterize response rates for mail surveys published in medical journals; to determine how the response rate among subjects who are typical targets of mail surveys varies; and to evaluate the contribution of several techniques used by investigators to enhance response rates. Methods. One hundred seventy-eight manuscripts published in 1991, representing 321 distinct mail surveys, were abstracted to determine response rates and survey techniques. In a follow-up mail survey, 113 authors of these manuscripts provided supplementary information. Results. The mean response rate among mail surveys published in medical journals is approximately 60%. However, response rates vary according to subject studied and techniques used. Published surveys of physicians have a mean response rate of only 54%, and those of non-physicians have a mean response rate of 68%. In addition, multivariable models suggest that written reminders provided with a copy of the instrument and telephone reminders are each associated with response rates about 13% higher than surveys that do not use these techniques. Other techniques, such as anonymity and financial incentives, are not associated with higher response rates. Conclusions. Although several mail survey techniques are associated with higher response rates, response rates to published mail surveys tend to be moderate. However, a survey's response rate is at best an indirect indication of the extent of non-respondent bias. Investigators, journal editors, and readers should devote more attention to assessments of bias, and less to specific response rate thresholds. (C) 1997 Elsevier Science Inc. C1 UNIV CHICAGO,DEPT SOCIOL,CHICAGO,IL 60637. VET AFFAIRS MED CTR,PHILADELPHIA,PA 19104. UNIV PENN,SCH MED,DIV GEN INTERNAL MED,PHILADELPHIA,PA 19104. RP Asch, DA (reprint author), UNIV CHICAGO,MED CTR,DIV GEN INTERNAL MED,GEN INTERNAL MED SECT,5841 S MARYLAND AVE,MC 6098,CHICAGO,IL 60637, USA. RI Christakis, Nicholas/B-6690-2008; Christakis, Nicholas/C-3205-2009 NR 19 TC 1339 Z9 1347 U1 2 U2 41 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0895-4356 J9 J CLIN EPIDEMIOL JI J. Clin. Epidemiol. PD OCT PY 1997 VL 50 IS 10 BP 1129 EP 1136 DI 10.1016/S0895-4356(97)00126-1 PG 8 WC Health Care Sciences & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA YA673 UT WOS:A1997YA67300008 PM 9368521 ER PT J AU Gukovskaya, AS Gukovsky, I Zaninovic, V Song, M Sandoval, D Gukovsky, S Pandol, SJ AF Gukovskaya, AS Gukovsky, I Zaninovic, V Song, M Sandoval, D Gukovsky, S Pandol, SJ TI Pancreatic acinar cells produce, release, and respond to tumor necrosis factor-alpha - Role in regulating cell death and pancreatitis SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE TNF alpha; apoptosis; NF-kappa B; TNF alpha receptor; pancreatitis ID PLATELET-ACTIVATING-FACTOR; NF-KAPPA-B; TNF-ALPHA; MONOCLONAL-ANTIBODIES; MOLECULAR MECHANISMS; FACTOR RECEPTORS; EXPRESSION; APOPTOSIS; CYTOKINE; RAT AB The aim of this study was to determine whether tumor necrosis factor-alpha (TNF alpha) and receptors for TNF alpha are expressed in the exocrine pancreas, and whether pancreatic acinar cells release and respond to TNF alpha. Reverse transcription PCR, immunoprecipitation, and Western blot analysis demonstrated the presence of TNF alpha and 55- and 75-kD TNF alpha receptors in pancreas from control rats, rats with experimental pancreatitis induced by supramaximal doses of cerulein, and in isolated pancreatic acini. Immunohistochemistry showed TNF alpha presence in pancreatic acinar cells. ELISA and bioassay measurements of TNF alpha indicated its release from pancreatic acinar cells during incubation in primary culture. Acinar cells responded to TNF alpha. TNF alpha potentiated NF-kappa B translocation into the nucleus and stimulated apoptosis in isolated acini while not affecting LDH release, In vivo studies demonstrated that neutralization of TNF alpha with an antibody produced a mild improvement in the parameters of cerulein-induced pancreatitis. However, TNF alpha neutralization greatly inhibited apoptosis in a modification of the cerulein model of pancreatitis which is associated with a high percentage of apoptotic cell death. The results indicate that pancreatic acinar cells produce, release, and respond to TNF alpha. This cytokine regulates apoptosis in both isolated pancreatic acini and experimental pancreatitis. C1 UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90073. RP Gukovskaya, AS (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,11301 WILSHIRE BLVD,BLDG 258,ROOM 340,LOS ANGELES,CA 90073, USA. NR 56 TC 272 Z9 281 U1 1 U2 10 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD OCT 1 PY 1997 VL 100 IS 7 BP 1853 EP 1862 DI 10.1172/JCI119714 PG 10 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA YA424 UT WOS:A1997YA42400025 PM 9312187 ER PT J AU ElZimaity, HMT Itani, K Graham, DY AF ElZimaity, HMT Itani, K Graham, DY TI Early diagnosis of signet ring cell carcinoma of the stomach: role of the Genta stain SO JOURNAL OF CLINICAL PATHOLOGY LA English DT Article DE signet ring cell; Genta stain; gastric carcinoma; early diagnosis ID GASTRIC-CARCINOMA; YOUNG AB Signet ring cell carcinoma is a poorly differentiated adenocarcinoma in which the tumour cells invade singly or in small groups. Early stages of the disease can be missed easily when using regular haematoxylin and eosin staining. This is a report of a case in which routine screening of gastric biopsies with the Genta stain was responsible for rapid identification of signet ring carcinoma. The patient, a 29 year old woman, had a large portion of the antrum excised surgically for signet ring cell gastric carcinoma. Follow up endoscopy six years later showed no evidence of tumour. Twenty six large cup biopsies were obtained and a single focus of signet ring tumour cells infiltrating the surface mucosa in single files was seen. The diagnosis was missed on haematoxylin and eosin stain by three senior pathologists but owing to the Alcian blue component of the Genta stain the tumour cells were recognised easily. Thus, the Genta stain not only facilitates detection of Helicobacter pylori but also allows for simultaneous visualisation of gastric morphology as well as signet ring carcinoma that can be missed with conventional stains. C1 BAYLOR COLL MED,VA MED CTR,DEPT SURG,HOUSTON,TX 77030. RP ElZimaity, HMT (reprint author), BAYLOR COLL MED,VA MED CTR 111D,DEPT MED,2002 HOLCOMBE BLVD,ROOM 3A352,HOUSTON,TX 77030, USA. NR 14 TC 13 Z9 14 U1 0 U2 0 PU BRITISH MED JOURNAL PUBL GROUP PI LONDON PA BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON, ENGLAND WC1H 9JR SN 0021-9746 J9 J CLIN PATHOL JI J. Clin. Pathol. PD OCT PY 1997 VL 50 IS 10 BP 867 EP 868 DI 10.1136/jcp.50.10.867 PG 2 WC Pathology SC Pathology GA YC587 UT WOS:A1997YC58700017 PM 9462273 ER PT J AU Kern, RS Marshall, BD Kuehnel, TG Mintz, J Hayden, JL Robertson, MJ Green, MF AF Kern, RS Marshall, BD Kuehnel, TG Mintz, J Hayden, JL Robertson, MJ Green, MF TI Effects of risperidone on polydipsia in chronic schizophrenia patients SO JOURNAL OF CLINICAL PSYCHOPHARMACOLOGY LA English DT Letter ID PSYCHIATRIC-PATIENTS; HYPONATREMIA; CLOZAPINE C1 CAMARILLO STATE HOSP & DEV CTR,CAMARILLO,CA. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. RP Kern, RS (reprint author), UNIV CALIF LOS ANGELES,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024, USA. FU NIMH NIH HHS [MH-30911] NR 12 TC 7 Z9 7 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0271-0749 J9 J CLIN PSYCHOPHARM JI J. Clin. Psychopharmacol. PD OCT PY 1997 VL 17 IS 5 BP 432 EP 435 DI 10.1097/00004714-199710000-00021 PG 4 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA XY987 UT WOS:A1997XY98700022 PM 9316001 ER PT J AU Mendez, MF Perryman, KM Miller, BL Swartz, JR Cummings, JL AF Mendez, MF Perryman, KM Miller, BL Swartz, JR Cummings, JL TI Compulsive behaviors as presenting symptoms of frontotemporal dementia SO JOURNAL OF GERIATRIC PSYCHIATRY AND NEUROLOGY LA English DT Article ID FRONTAL-LOBE DEMENTIA; CEREBRAL BLOOD-FLOW; NON-ALZHEIMER TYPE; CAUDATE-NUCLEUS; PICKS-DISEASE; DISORDER; DEGENERATION; PROVOCATION; SPECT; SCALE AB Frontotemporal dementia (FTD) is a common neurodegenerative dementia syndrome. Compulsive behaviors frequently occur in FTD and may be presenting symptoms of this disorder. This study evaluated compulsive behaviors as presenting symptoms in 29 patients with FTD compared to 48 patients with Alzheimer's disease (ADI enrolled in the UCLA Alzheimer's Disease Center, The FTD patients met the Lund and Manchester criteria for FTD and had predominant frontal hypoperfusion on single-photon emission computer tomography neuroimaging. The AD patients met National Institute of Neurological and Communicative Disorders-Alzheimer's Disease and Related Disorders criteria for clinically probable AD. Compulsive behaviors occurred in 11 FTD patients (38%) versus 5 AD patients (10%) (chi(2) = 6.73, P < .01). This difference persisted after controlling for the younger age of the FTD group. There was a range of compulsive behaviors, with the most frequent being repetitive checking activities. Compulsive behaviors are common presenting symptoms among FTD patients and may result from an inability to inhibit urges to perform compulsive movements from damage to frontal-striatal circuits. C1 W Los Angeles Vet Affairs Med Ctr, Neurobehav Unit 691 116AF, Los Angeles, CA 90073 USA. Univ Calif Los Angeles, Dept Neurol, Los Angeles, CA 90024 USA. Univ Calif Los Angeles, Dept Psychiat & Biobehav Sci, Los Angeles, CA 90024 USA. Harbor UCLA Med Ctr, Los Angeles, CA USA. RP Mendez, MF (reprint author), W Los Angeles Vet Affairs Med Ctr, Neurobehav Unit 691 116AF, 11301 Wilshire Blvd, Los Angeles, CA 90073 USA. FU NIA NIH HHS [AG10123] NR 41 TC 50 Z9 50 U1 0 U2 2 PU DECKER PERIODICALS INC PI HAMILTON PA 4 HUGHSON STREET SOUTH PO BOX 620, LCD 1, HAMILTON, ONTARIO L8N 3K7, CANADA SN 0891-9887 J9 J GERIATR PSYCH NEUR JI J. Geriatr. Psychiatry Neurol. PD OCT PY 1997 VL 10 IS 4 BP 154 EP 157 PG 4 WC Geriatrics & Gerontology; Clinical Neurology; Psychiatry SC Geriatrics & Gerontology; Neurosciences & Neurology; Psychiatry GA YQ720 UT WOS:000071416500004 PM 9453681 ER PT J AU Pascualy, M Tsuang, D Shores, M Agustin, C Krause, E Spain, W Bird, T Peskind, E AF Pascualy, M Tsuang, D Shores, M Agustin, C Krause, E Spain, W Bird, T Peskind, E TI Frontal-complex partial status epilepticus misdiagnosed as bipolar affective disorder in a 75-year-old man SO JOURNAL OF GERIATRIC PSYCHIATRY AND NEUROLOGY LA English DT Article ID PARTIAL SEIZURES; ORIGIN AB The incidence and prevalence of epilepsy increases with age, with the majority of cases having a known cause, and approximately half of elderly patients with epilepsy experiencing complex partial seizures that often present initially as neuropsychiatric symptoms. This presentation often delays diagnosis of the epileptiform disorder. To illustrate, we present the case of a 75-year-old man who was initially misdiagnosed with bipolar affective disorder later to be revealed as a frontal lobe seizure disorder. C1 VA Puget Sound Hlth Care Syst, Mental Hlth Serv 116, Seattle, WA 98108 USA. VA Puget Sound Hlth Care Syst, Neurol Serv, Seattle, WA 98108 USA. VA Puget Sound Hlth Care Syst, Ctr Geriatr Res Educ & Clin, Seattle, WA 98108 USA. Univ Washington, Sch Med, Dept Psychiat & Behav Sci, Seattle, WA 98195 USA. RP Pascualy, M (reprint author), VA Puget Sound Hlth Care Syst, Mental Hlth Serv 116, 1660 S Columbian Way, Seattle, WA 98108 USA. RI Tsuang, Debby/L-7234-2016 OI Tsuang, Debby/0000-0002-4716-1894 NR 12 TC 2 Z9 3 U1 0 U2 0 PU DECKER PERIODICALS INC PI HAMILTON PA 4 HUGHSON STREET SOUTH PO BOX 620, LCD 1, HAMILTON, ONTARIO L8N 3K7, CANADA SN 0891-9887 J9 J GERIATR PSYCH NEUR JI J. Geriatr. Psychiatry Neurol. PD OCT PY 1997 VL 10 IS 4 BP 158 EP 160 PG 3 WC Geriatrics & Gerontology; Clinical Neurology; Psychiatry SC Geriatrics & Gerontology; Neurosciences & Neurology; Psychiatry GA YQ720 UT WOS:000071416500005 PM 9453682 ER PT J AU Schultz, D Su, XF Bishop, SP Billadello, J DellItalia, LJ AF Schultz, D Su, XF Bishop, SP Billadello, J DellItalia, LJ TI Selective induction of the creatine kinase-B gene in chronic volume overload hypertrophy is not affected by ACE-inhibitor therapy SO JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY LA English DT Article DE creatine kinase; mitral regurgitation; angiotensin converting enzyme inhibitor; congestive heart failure; left ventricular hypertrophy ID COMPLETE NUCLEOTIDE-SEQUENCE; CORONARY-ARTERY OCCLUSION; MITRAL REGURGITATION; CARDIAC-HYPERTROPHY; DEVELOPMENTAL REGULATION; MYOCARDIAL-INFARCTION; PRESSURE OVERLOAD; MESSENGER-RNAS; HEART-FAILURE; EXPRESSION AB Hypertrophied and failing myocardium has been shown to undergo creatine kinase. (CK) isoform switching, resulting in increased MB and BB components, We tested the hypothesis that chronic volume overload hypertrophy due to mitral regurgitation in the dog causes CK isoenzyme switching and that this could be reversed by angiotensin converting enzyme inhibitor therapy. Thirteen adult mongrel dogs had mitral regurgitation induced by mitral valvular chordal rupture: six were treated with ramipril for 4 months and seven were untreated for 4 months. Twelve dogs were sham-operated: six received ramipril for 3 months and six were untreated. Left ventricular end-diastolic volume increased from 58 +/- 4 to 104 +/- 10 ml in untreated (P<0.001) and from 55 +/- 3 to 91 +/- 6 ml in treated dogs (P<0.01) as LV mass/volume ratio decreased in both untreated (1.60 +/- 0.07 to 1.13 +/- 0.08 g/ml, P<0.001) and treated dogs (1.44 +/- 0.06 to 1.20 +/- 0.08 g/ml, P<0.01). CK-MB isoform was 7.4 +/- 1.1% in normal shams and increased to 13.5 +/- 1.9% and 18.1 +/- 3.0% in both treated and untreated mitral regurgitation dogs; respectively (P<0.05). Steady state CK-B mRNA increased three fold in treated and untreated dogs with mitral regurgitation (P<0.003) compared to normals, while CK-M mRNA expression did not differ in all groups. Thus, chronic volume overload hypertrophy of mitral regurgitation induces CK isoform switching by selective induction of the CK-B gene, and ramipril therapy does not affect this isoform switch. This may reflect a response to increased diastolic stress and more efficient energy utilization in the volume overloaded myocardium. (C) 1997 Academic Press Limited. C1 UNIV ALABAMA, BIRMINGHAM VET AFFAIRS MED CTR, DEPT MED, DIV CARDIOVASC DIS HYPERTENS & VASC BIOL, BIRMINGHAM, AL 35294 USA. AUBURN COLL VET MED, AUBURN, AL 36849 USA. WASHINGTON UNIV, DEPT MED, DIV CARDIOVASC DIS, ST LOUIS, MO 63110 USA. FU NHLBI NIH HHS [HL54816, 2T32HL07457-16A1]; NICHD NIH HHS [HD SCOR HL 17667] NR 39 TC 10 Z9 10 U1 2 U2 2 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0022-2828 EI 1095-8584 J9 J MOL CELL CARDIOL JI J. Mol. Cell. Cardiol. PD OCT PY 1997 VL 29 IS 10 BP 2665 EP 2673 DI 10.1006/jmcc.1997.0498 PG 9 WC Cardiac & Cardiovascular Systems; Cell Biology SC Cardiovascular System & Cardiology; Cell Biology GA YC586 UT WOS:A1997YC58600007 PM 9344761 ER PT J AU Dawson, TM Starkebaum, G AF Dawson, TM Starkebaum, G TI Colchicine induced rhabdomyolysis SO JOURNAL OF RHEUMATOLOGY LA English DT Article DE colchicine; rhabdomyolysis; gout ID FAMILIAL MEDITERRANEAN FEVER; RENAL-FAILURE; MYOPATHY; NEUROPATHY AB A 59-year-old man with chronic obstructive pulmonary disease (COPD)? atrial fibrillation, and gout developed acute dyspnea, cough, and diffuse muscle aches and pains. He had commenced colchicine (0.6 mg bid po), for the first time, one month earlier for recurrent gout attacks. Clinical examination revealed atrial fibrillation, an exacerbation of his pulmonary disease, render muscles, especially calves, and diffuse muscle weakness. Laboratory results included creatinine phosphokinase 6961 U/l (1% MB), microscopic hematuria, myoglobinuria, elevated creatinine 1.6 mg/dl, and blood urea nitrogen 17 mg/dl. COPD and atrial fibrillation were treated and colchicine was discontinued. The patient made a full recovery. This 2nd reported case of colchicine induced rhabdomyolysis is the first reported in the treatment of gout. C1 UNIV WASHINGTON,DIV RHEUMATOL,SEATTLE,WA 98195. RP Dawson, TM (reprint author), VA PUGET SOUND HLTH CARE SYST,MED SERV,DEPT MED,ARTHRITIS SECT,1600 S COLUMBIAN WAY,SEATTLE,WA 98108, USA. NR 13 TC 18 Z9 19 U1 0 U2 0 PU J RHEUMATOL PUBL CO PI TORONTO PA 920 YONGE ST, SUITE 115, TORONTO ON M4W 3C7, CANADA SN 0315-162X J9 J RHEUMATOL JI J. Rheumatol. PD OCT PY 1997 VL 24 IS 10 BP 2045 EP 2046 PG 2 WC Rheumatology SC Rheumatology GA XY693 UT WOS:A1997XY69300033 PM 9330953 ER PT J AU Khuri, SF Daley, J Henderson, W Hur, K Gibbs, JO Barbour, G Demakis, J Irvin, G Stremple, JF Grover, F McDonald, G Passaro, E Fabri, PJ Spencer, J Hammermeister, K Aust, JB AF Khuri, SF Daley, J Henderson, W Hur, K Gibbs, JO Barbour, G Demakis, J Irvin, G Stremple, JF Grover, F McDonald, G Passaro, E Fabri, PJ Spencer, J Hammermeister, K Aust, JB TI Risk adjustment of the postoperative mortality rate for the comparative assessment of the quality of surgical care: Results of the National Veterans Affairs Surgical Risk Study SO JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS LA English DT Article ID HOSPITAL DISCHARGE DATA; CARDIAC-SURGERY; CARDIOVASCULAR MEDICINE; PROGNOSTIC PREDICTION; OPERATIVE MORBIDITY; RELATIVE VALUES; OUTCOMES; INDEX; IMPROVEMENT; PATIENT AB Background: The National Veterans Affairs Surgical Risk Study was designed to collect reliable, valid data on patient risk and outcomes for major surgery in the Veterans Health Administration and to report comparative risk-adjusted postoperative mortality rates for surgical services in Veterans Health Administration. Study Design: This cohort study was conducted in 44 Veterans Affairs Medical Centers. Included were 87,078 major noncardiac operations performed under general, spinal, or epidural anesthesia between October 1, 1991, and December 31, 1993. The main outcomes measure was all-cause mortality within 30 days after the index procedure. Multivariable logistic regression risk-adjustment models for all operations and for eight surgical subspecialties were developed. Risk-adjusted surgical mortality rates were expressed as observed-to-expected ratios and were compared with unadjusted 30-day postoperative mortality rates. Results: Patient risk factors predictive of postoperative mortality included serum albumin level, American So-ciety of Anesthesia class, emergency operation, and 31 additional preoperative variables. Considerable variability in unadjusted mortality rates for all operations was observed across the 44 hospitals (1.2-5.4%). After risk adjustment, observed-to-expected ratios ranged from 0.49 to 1.53. Rank order correlation of the hospitals by unadjusted and risk-adjusted mortality rates for all operations was 0.64. Ninety-three percent of the hospitals changed rank after risk adjustment, 50% by more than 5 and 25% by more than 10. Conclusions: The Department of Veterans Affairs has successfully implemented a system for the prospective collection and comparative reporting of risk-adjusted postoperative mortality rates after major noncardiac operations. Risk adjustment had an appreciable impact on the rank ordering of the hospitals and provided a means for monitoring and potentially improving the quality of surgical care. (C) 1997 by the American College of Surgeons. C1 HARVARD UNIV,SCH MED,BOSTON,MA. BETH ISRAEL MED CTR,DIV GEN MED & PRIMARY CARE,BOSTON,MA. HINES VA CTR COOPERAT STUDIES HLTH SERV,HINES,IL. WASHINGTON VA MED CTR,WASHINGTON,DC. GEORGE WASHINGTON UNIV,SCH MED,DEPT MED,WASHINGTON,DC. UNIV MIAMI,DEPT SURG,MIAMI,FL 33152. MIAMI VA MED CTR,MIAMI,FL. PITTSBURGH VA MED CTR,PITTSBURGH,PA. UNIV PITTSBURGH,SCH MED,PITTSBURGH,PA. DENVER VA MED CTR,DENVER,CO. UNIV COLORADO,HLTH SCI CTR,DENVER,CO. DEPT VET AFFAIRS HEADQUARTERS,DEPT SURG,WASHINGTON,DC. UNIV CALIF LOS ANGELES,LOS ANGELES,CA. TAMPA VA MED CTR,TAMPA,FL. UNIV S FLORIDA,COLL MED,TAMPA,FL. UNIV TEXAS,HLTH SCI CTR,DEPT SURG,SAN ANTONIO,TX 78284. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA. RP Khuri, SF (reprint author), VET AFFAIRS MED CTR,1400 VFW PKWY,W ROXBURY,MA 02132, USA. NR 54 TC 429 Z9 431 U1 3 U2 7 PU AMER COLL SURGEONS PI CHICAGO PA 54 EAST ERIE ST, CHICAGO, IL 60611 SN 1072-7515 J9 J AM COLL SURGEONS JI J. Am. Coll. Surg. PD OCT PY 1997 VL 185 IS 4 BP 315 EP 327 DI 10.1016/S1072-7515(01)00938-3 PG 13 WC Surgery SC Surgery GA XZ460 UT WOS:A1997XZ46000001 PM 9328380 ER PT J AU Daley, J Khuri, SF Henderson, W Hur, K Gibbs, JO Barbour, G Demakis, J Irvin, G Stremple, JF Grover, F McDonald, G Passaro, E Fabri, PJ Spencer, J Hammermeister, K Aust, JB Oprian, C AF Daley, J Khuri, SF Henderson, W Hur, K Gibbs, JO Barbour, G Demakis, J Irvin, G Stremple, JF Grover, F McDonald, G Passaro, E Fabri, PJ Spencer, J Hammermeister, K Aust, JB Oprian, C TI Risk adjustment of the postoperative morbidity rate for the comparative assessment of the quality of surgical care: Results of the National Veterans Affairs Surgical Risk Study SO JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS LA English DT Article ID HOSPITAL DISCHARGE DATA; ARTERY BYPASS-SURGERY; CARDIAC-SURGERY; HEALTH-CARE; CONTINUOUS IMPROVEMENT; PROGNOSTIC PREDICTION; PROSPECTIVE PAYMENT; ADVERSE EVENTS; MORTALITY; COMPLICATIONS AB Background: The National Veterans Affairs Surgical Risk Study was designed to collect reliable, valid data on patient risk and outcomes for major surgery in the Veterans Health Administration and to report comparative risk-adjusted postoperative mortality and morbidity rates for surgical services in the Veterans Health Administration. Study Design: This was a cohort study conducted at 44 Veterans Affairs Medical Centers closely affiliated with university medical centers. Included were 87,078 major noncardiac operations performed under general, spinal, or epidural anesthesia between October 1, 1991, and December 31, 1993. The main outcomes measures in this report are 21 postoperative adverse events (morbidities) occurring within 30 days after the index procedure. Multivariable logistic regression risk-adjustment models for all operations and for eight surgical subspecialties were developed, Results: Patient risk factors predictive of postoperative morbidity included serum albumin level, American Society of Anesthesia class, the complexity of the operation, and 17 other preoperative risk variables. Wide variation in the unadjusted rates of one or more postoperative morbidities for all operations was observed across the 44 hospitals (7.4-28.4%). Risk-adjusted observed-to-expected ratios ranged from 0.49 to 1.46. The Spearman rank order correlation between the ranking of the hospitals based on unadjusted morbidity rates and risk-adjusted observed-to-expected ratios for all operations was 0.87. There was little or no correlation between the rank order of the hospitals by risk-adjusted morbidity and risk-adjusted mortality. Conclusions: The Department of Veterans Affairs has successfully implemented a system for the prospective collection and comparative reporting of postoperative mortality and morbidity rates after major noncardiac operations. Risk adjustment had only a modest effect on the rank order of the hospitals. (C) 1997 by the American College of Surgeons. C1 HARVARD UNIV,SCH MED,BOSTON,MA. BETH ISRAEL MED CTR,DIV GEN MED & PRIMARY CARE,BOSTON,MA. BRIGHAM & WOMENS HOSP,BOSTON,MA 02115. HINES VA CTR COOPERAT STUDIES HLTH SERV,HINES,IL. WASHINGTON VA MED CTR,WASHINGTON,DC. GEORGE WASHINGTON UNIV,SCH MED,WASHINGTON,DC. UNIV MIAMI,DEPT SURG,MIAMI,FL 33152. UNIV MIAMI,SCH MED,MIAMI,FL 33152. MIAMI VA MED CTR,MIAMI,FL. PITTSBURGH VA MED CTR,PITTSBURGH,PA. UNIV PITTSBURGH,SCH MED,PITTSBURGH,PA. DENVER VA MED CTR,DENVER,CO. UNIV COLORADO,HLTH SCI CTR,DENVER,CO. DEPT VET AFFAIRS HEADQUARTERS,DEPT SURG,WASHINGTON,DC. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,LOS ANGELES,CA. TAMPA VA MED CTR,TAMPA,FL. UNIV S FLORIDA,COLL MED,TAMPA,FL. UNIV TEXAS,HLTH SCI CTR,DEPT SURG,SAN ANTONIO,TX 78284. RP Daley, J (reprint author), BROCKTON W ROXBURY VET AFFAIRS MED CTR,1400 VFW PKWY,W ROXBURY,MA 02132, USA. NR 53 TC 342 Z9 346 U1 4 U2 7 PU AMER COLL SURGEONS PI CHICAGO PA 54 EAST ERIE ST, CHICAGO, IL 60611 SN 1072-7515 J9 J AM COLL SURGEONS JI J. Am. Coll. Surg. PD OCT PY 1997 VL 185 IS 4 BP 328 EP 340 DI 10.1016/S1072-7515(01)00939-5 PG 13 WC Surgery SC Surgery GA XZ460 UT WOS:A1997XZ46000002 PM 9328381 ER PT J AU Mobley, C Saunders, MJ AF Mobley, C Saunders, MJ TI Oral health screening guidelines for nondental health care providers (Reprinted) SO JOURNAL OF THE AMERICAN DIETETIC ASSOCIATION LA English DT Reprint AB Clues identified in oral health screening can be associated with medical conditions, medical treatments, and overall health status. Thorough oral health screening involves not only talking with the patient, but touching and exploring the mouth. Oral health screening expands the role of dietitians in improving the nutritional and dietary practices of clients. C1 UNIV TEXAS,HLTH SCI CTR,DEPT DENT DIAGNOST SCI,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. S TEXAS VET HLTH CARE SYST,AUDIE L MURPHY DIV,CTR GERIATR RES EDUC & CLIN,SAN ANTONIO,TX. RP Mobley, C (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT COMMUNITY DIS,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. NR 10 TC 1 Z9 1 U1 0 U2 0 PU AMER DIETETIC ASSOC PI CHICAGO PA 216 W JACKSON BLVD #800, CHICAGO, IL 60606-6995 SN 0002-8223 J9 J AM DIET ASSOC JI J. Am. Diet. Assoc. PD OCT PY 1997 VL 97 IS 10 SU 2 BP S123 EP S126 DI 10.1016/S0002-8223(97)00745-1 PG 4 WC Nutrition & Dietetics SC Nutrition & Dietetics GA XZ571 UT WOS:A1997XZ57100007 PM 9336571 ER PT J AU Patel, A Dorey, F Franklin, J deKernion, JB AF Patel, A Dorey, F Franklin, J deKernion, JB TI Recurrence patterns after radical retropubic prostatectomy: Clinical usefulness of prostate specific antigen doubling times and log slope prostate specific antigen SO JOURNAL OF UROLOGY LA English DT Article DE prostate; prostate-specific antigen; prostatectomy; linear models; recurrence ID LOCAL RECURRENCE; CANCER; ADENOCARCINOMA; PROGRESSION; LEUPROLIDE; FLUTAMIDE; THERAPY; RATES AB Purpose: We studied the correlation between prostate specific antigen (PSA) doubling time or, equivalently, log slope PSA and clinical recurrence in patients with detectable PSA after radical retropubic prostatectomy who were followed expectantly. Materials and Methods: In patients with PSA recurrence after radical retropubic prostatectomy log slope PSA was determined from the difference in the 2 log PSA values divided by the time between readings in months. For a given slope the corresponding PSA doubling time was calculated as log x 2 divided by the slope of the log PSA line. When the initial PSA value was considerably greater than 0.4 ng./ml., the log slope PSA plot was extrapolated to determine the time point at which PSA would have become detectable (0.4 ng./ml.). The relationship between these values, and the time and pattern of clinical recurrence were studied. Results: In this series of 77 patients 80% with PSA doubling time of 6 months or greater remained clinically disease-free compared to 64% with PSA doubling time less than 6 months. PSA doubling time had better correlation with time to clinical recurrence after PSA became detectable (p <0.001 Cox proportional hazards model) than Gleason sum, pathological stage or margin status. Biochemical recurrence within 3 months was associated with early clinical recurrence (p <0.002). In addition, short PSA doubling time, that is a high log slope, regardless of the time at which PSA became positive was strongly associated with clinical recurrence (p <0.001). Distant recurrence was invariably associated with short PSA doubling time. Conversely, local recurrence reliably correlated with long PSA doubling time, that is a low log slope. Conclusions: After PSA became detectable PSA doubling time or, equivalently, log slope PSA, was a better indicator of the risk and time to clinical recurrence after radical retropubic prostatectomy than preoperative PSA, specimen Gleason sum or pathological stage. Hormone treatment may be targeted to patients at high risk for early metastatic clinical recurrence, appropriately timed radiation can be offered for proved local recurrence in those with long PSA doubling time and expectant treatment may be proposed for those with long PSA doubling time who remain clinically disease-free. Frequent and expensive imaging does not appear to be cost-effective in this latter group. C1 UNIV CALIF LOS ANGELES,DEPT UROL ONCOL & BIOSTAT,LOS ANGELES,CA. W LOS ANGELES VET AFFAIRS MED CTR,DEPT UROL ONCOL,LOS ANGELES,CA 90073. RP Patel, A (reprint author), UNIV CALIF LOS ANGELES,DEPT UROL,LOS ANGELES,CA 90024, USA. NR 21 TC 207 Z9 211 U1 1 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-5347 J9 J UROLOGY JI J. Urol. PD OCT PY 1997 VL 158 IS 4 BP 1441 EP 1445 DI 10.1016/S0022-5347(01)64238-1 PG 5 WC Urology & Nephrology SC Urology & Nephrology GA XV524 UT WOS:A1997XV52400034 PM 9302139 ER PT J AU Liu, HM Wu, XY Xiao, HL Conway, JA Kappes, JC AF Liu, HM Wu, XY Xiao, HL Conway, JA Kappes, JC TI Incorporation of functional human immunodeficiency virus type 1 integrase into virions independent of the Gag-Pol precursor protein SO JOURNAL OF VIROLOGY LA English DT Article ID VIRAL NUCLEIC-ACIDS; REVERSE-TRANSCRIPTASE; DNA INVITRO; GENETIC-ANALYSIS; CELL-LINE; EXPRESSION; MUTANTS; LOCALIZATION; REPLICATION; INFECTION AB Retroviral integrase (IN) is expressed and incorporated into virions as part of the Gag-Pol polyprotein precursor. IN catalyzes integration of the proviral DNA into host cell chromosomes during the early stages of the virus life cycle, and as a component of Gag-Pol, it is involved in virion morphogenesis during late stages. It is unknown whether the scheme, conserved among retroviruses, for expressing and incorporating IN as a component of the Gag-Pol precursor protein is necessary for its function in the infected cell after viral entry. We have developed human immunodeficiency virus (HIV) virion-associated accessory proteins (Vpr and Vpx) as vehicles to deliver both foreign and viral proteins into the virus particle by their expression in trans as heterologous fusion proteins (X. Wu, et al., J. virol. 69:3389-3398, 1995; X. Wu, et al., J. Virol. 70:3378-3384, 1996; X. Wu, et al., EMBO J. 16:5113-5122, 1977). To analyze IN function independent of its expression as a part of Gag-Pol, we expressed and incorporated IN into HIV type 1 (HIV-1) virions in trans as a fusion partner of Vpr (Vpr-IN). Our results demonstrate that the Vpr-IN fusion protein is efficiently incorporated into virions and then processed by the viral protease to liberate the IN protein. Virus derived from IN-minus provirus is noninfectious. However, this defect is overcome by trans complementation with the Vpr-IN fusion protein. Moreover, complemented virions are able to replicate through a complete cycle of infection, included formation of the provirus (integration). These results show, for the first time, that full IN function can be provided in trans, independent of its expression and incorporation into virions as a component of Gag-Pol. This finding also indicates that the IN domain of Gag-Pol is not required for the formation of infectious virions when IN is provided in trans. The ability to incorporate functional IN into retroviral particles in trans will provide unique opportunities to explore the function of this critical enzyme in a biologically relevant context, i.e., in infected cells as part of the nucleoprotein/preintegration complex. C1 UNIV ALABAMA,DEPT MED,BIRMINGHAM,AL 35294. UNIV ALABAMA,DEPT MICROBIOL,BIRMINGHAM,AL 35294. BIRMINGHAM VET AFFAIRS MED CTR,RES SERV,BIRMINGHAM,AL 35233. FU NIAID NIH HHS [P30-AI-27767, AI31816] NR 32 TC 28 Z9 28 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD OCT PY 1997 VL 71 IS 10 BP 7704 EP 7710 PG 7 WC Virology SC Virology GA XW142 UT WOS:A1997XW14200068 PM 9311854 ER PT J AU Geller, SE Bernstein, SJ Harlow, SD AF Geller, SE Bernstein, SJ Harlow, SD TI The decision-making process for the treatment of abnormal uterine bleeding SO JOURNAL OF WOMENS HEALTH LA English DT Article ID SMALL-AREA VARIATIONS; MEFENAMIC-ACID; UNITED-STATES; MEDICAL-CARE; HYSTERECTOMY; OUTCOMES; PATIENT; MENORRHAGIA; PREFERENCES; INFORMATION AB This pilot study was conducted to investigate the treatment decision-making process of patients and physicians for abnormal uterine bleeding (AUB). Frequently, women with AUB are referred for hysterectomy without diagnostic workup, alternative therapeutic management, or patient input (i.e., patient treatment preferences). Variations in treatment strategies used for patients may be related to a number of factors external to the patient's underlying disease. However, little is known about which factors are most influential or about the extent to which they influence physicians',and patients' decisions. We prospectively followed the management and treatment of 52 women with complaints of AUB and examined differences in treatment among these patients. Extensive previsit interviews were conducted with these women to identify each patient's symptoms, health status, functional status, and preferences for and expectations of treatment. We then conducted telephone interviews within a week of the visit and again 9-12 months later to determine the treatment plan, patient level of participation in and satisfaction with the treatment, symptoms, and functional status. Overall, our findings suggest that patients want to be involved in making treatment decisions and that when women were presented with alternatives to hysterectomy, many chose alternative medical therapy or other surgical procedures. In addition, women reported that these alternative treatments produced significant improvement in symptom intensity and functioning. Increased patient participation in decision making enhanced patient satisfaction. These findings suggest that hysterectomy rates may be decreased by offering women alternative treatments and by finding ways to increase women's participation in their treatment decisions. C1 UNIV MICHIGAN,SCH MED,DEPT INTERNAL MED,ANN ARBOR,MI. US DEPT VET AFFAIRS,ANN ARBOR,MI. UNIV MICHIGAN,SCH PUBL HLTH,DEPT EPIDEMIOL,ANN ARBOR,MI 48109. RP Geller, SE (reprint author), UNIV ILLINOIS,SCH PUBL HLTH,DEPT COMMUNITY HLTH SCI,2035 W TAYLOR,CHICAGO,IL 60612, USA. NR 33 TC 13 Z9 13 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 1059-7115 J9 J WOMENS HEALTH JI J. Womens Health PD OCT PY 1997 VL 6 IS 5 BP 559 EP 567 DI 10.1089/jwh.1997.6.559 PG 9 WC Public, Environmental & Occupational Health; Medicine, General & Internal; Obstetrics & Gynecology; Women's Studies SC Public, Environmental & Occupational Health; General & Internal Medicine; Obstetrics & Gynecology; Women's Studies GA YE674 UT WOS:A1997YE67400012 PM 9356979 ER PT J AU Shapiro, JI AF Shapiro, JI TI Pathogenesis of cardiac dysfunction during metabolic acidosis: Therapeutic implications SO KIDNEY INTERNATIONAL LA English DT Article; Proceedings Paper CT Conference Honoring Dr. Robert W Schrier CY NOV 09, 1996 CL DENVER, CO DE intracellular pH; adenosine triphosphate; acidosis; mitochondria; cardiac function ID NUCLEAR MAGNETIC-RESONANCE; HYPOXIC LACTIC-ACIDOSIS; SODIUM-BICARBONATE; SYSTEMIC ACIDOSIS; SKELETAL-MUSCLE; ISOLATED HEART; PH RESPONSES; CARBICARB; RAT; BRAIN C1 UNIV COLORADO,SCH MED,DEPT MED,DENVER VA MED CTR,DENVER,CO. UNIV COLORADO,SCH MED,DEPT RADIOL,DENVER,CO. UNIV COLORADO,SCH MED,WEBB WARING INST BIOMED RES,GILES FILLEY RES LAB,DENVER,CO. NR 33 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 SN 0085-2538 J9 KIDNEY INT JI Kidney Int. PD OCT PY 1997 SU 61 BP S47 EP S51 PG 5 WC Urology & Nephrology SC Urology & Nephrology GA XX137 UT WOS:A1997XX13700014 ER PT J AU Klingenspor, M Bodnar, J Welch, C Xia, XR Lusis, AJ Reue, K AF Klingenspor, M Bodnar, J Welch, C Xia, XR Lusis, AJ Reue, K TI Localization of ubiquitin gene family members to mouse Chromosomes 5, 11, and 18 SO MAMMALIAN GENOME LA English DT Article ID POLYUBIQUITIN GENE; UBB C1 W LOS ANGELES VET AFFAIRS MED CTR,LIPID RES LAB,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,INST MOL BIOL,LOS ANGELES,CA 90095. UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90095. UNIV CALIF LOS ANGELES,DEPT MICROBIOL & MOL GENET,LOS ANGELES,CA 90095. RI Klingenspor, Martin/D-6930-2011 OI Klingenspor, Martin/0000-0002-4502-6664 FU NHLBI NIH HHS [HL28481] NR 10 TC 1 Z9 1 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0938-8990 J9 MAMM GENOME JI Mamm. Genome PD OCT PY 1997 VL 8 IS 10 BP 789 EP 790 DI 10.1007/s003359900572 PG 2 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA XY081 UT WOS:A1997XY08100022 PM 9321482 ER PT J AU Gulli, G Rossetti, L DeFronzo, RA AF Gulli, G Rossetti, L DeFronzo, RA TI Hyperamylinemia is associated with hyperinsulinemia in the glucose-tolerant, insulin-resistant offspring of two Mexican-American non-insulin-dependent diabetic parents SO METABOLISM-CLINICAL AND EXPERIMENTAL LA English DT Article ID ISLET AMYLOID POLYPEPTIDE; PERFUSED RAT PANCREAS; BETA-CELL; EUGLYCEMIC CLAMP; INCREASED RISK; AMYLIN; SECRETION; SENSITIVITY; MELLITUS; INVIVO AB Several investigations have presented evidence that amylin inhibits insulin Secretion and induces insulin resistance both in vitro and in vivo. However, basal and postmeal amylin concentrations proved similar in non-insulin-dependent diabetes mellitus (NIDDM) patients and controls. Since hyperglycemia may alter both amylin and insulin secretion, we examined basal and glucose-stimulated amylin secretion in eight glucose-tolerant, insulin-resistant Mexican-American subjects with both parents affected with NIDDM (offspring) and correlated the findings with the insulin sensitivity data acquired by an insulin clamp. Eight offspring and eight Mexican-Americans without any family history of diabetes (controls) underwent measurement of fat free mass ((H2O)-H-3 dilution method), 180-minutes, 75-g oral glucose tolerance test (OGTT), and 40-mU/m(2), 180-minute euglycemic insulin clamp associated with H-3 - glucose infusion and indirect calorimetry. Fasting amylin was significantly increased in offspring versus controls (11.5 +/- 1.4 v 7.0 +/- 0.8 pmol/L, P < .05). After glucose ingestion, both total (3,073 +/- 257 v 1,870 +/- 202 pmol.L-1.min(-1), P < .01) and incremental (1,075 +/- 170 v 518 +/- 124 pmol.L-1.min(-1), P < .05) arias under the curve (AUCs) of amylin concentration were significantly greater in offspring. The amylin to insulin molar ratio was similar in offspring and controls at all time points. Basal and postglucose insulin and C-peptide concentrations were significantly increased in the offspring. No correlation was found between fasting amylin, postglucose amylin AUC or IAUC, and any measured parameter of glucose metabolism during a euglycemic-hyperinsulinemic clamp (total glucose disposal, 7.21 +/- 0,73 v 11.03 +/- 0.54; P < .001; nonoxidative glucose disposal, 3.17 +/- 0.59 v 6.33 +/- 0.56, P < .002; glucose oxidation, 4.05 +/- 0.46 v 4.71 +/- 0.21, P = NS; hepatic glucose production, 0.29 +/- 0.16 v 0.01, +/- 0.11, = NS; all mg.min(-1).kg(-1) fat-free mass, offspring v controls). In conclusion, these data do not support a causal role for amylin in the genesis of insulin resistance in NIDDM. Copyright (C) 1997 by W.B. Saunders Company. C1 UNIV TEXAS,HLTH SCI CTR,DIV DIABET,SAN ANTONIO,TX. YESHIVA UNIV,ALBERT EINSTEIN COLL MED,DIV ENDOCRINOL,BRONX,NY. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. FU NCRR NIH HHS [M01-RR-01346]; NIDDK NIH HHS [DK-24092] NR 47 TC 12 Z9 12 U1 1 U2 2 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0026-0495 J9 METABOLISM JI Metab.-Clin. Exp. PD OCT PY 1997 VL 46 IS 10 BP 1157 EP 1161 DI 10.1016/S0026-0495(97)90209-2 PG 5 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XY568 UT WOS:A1997XY56800011 PM 9322799 ER PT J AU Rettig, MB AF Rettig, MB TI Interleukin-6: An antagonizing problem becomes a solution SO NATURE BIOTECHNOLOGY LA English DT News Item ID ANTI-INTERLEUKIN-6 MONOCLONAL-ANTIBODY; MULTIPLE-MYELOMA; CYTOKINE; MICE RP Rettig, MB (reprint author), UNIV CALIF LOS ANGELES,SCH MED,W LOS ANGELES VET AFFAIRS MED CTR,DIV HEMATOL ONCOL,LOS ANGELES,CA 90073, USA. NR 14 TC 3 Z9 3 U1 0 U2 0 PU NATURE PUBLISHING CO PI NEW YORK PA 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 SN 1087-0156 J9 NAT BIOTECHNOL JI Nat. Biotechnol. PD OCT PY 1997 VL 15 IS 10 BP 952 EP 953 DI 10.1038/nbt1097-952 PG 2 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA XZ285 UT WOS:A1997XZ28500020 PM 9335041 ER PT J AU Loovis, CF Schall, DG Teter, DL AF Loovis, CF Schall, DG Teter, DL TI The role of assistive devices in the rehabilitation of hearing impairment SO OTOLARYNGOLOGIC CLINICS OF NORTH AMERICA LA English DT Article ID BENEFIT AB Formal investigation and clinical experience have revealed success in the use of assistive devices in aiding speech understanding where conventional amplification alone has failed. The long-term benefits of this technology are keeping people, particularly the elderly, socially engaged and independent. To accomplish this end, hearing health care professionals have not only to arm themselves with a rapidly advancing, complex array of technical tools, but also to apply them efficaciously. This article provides an overview of these tools, the procedures for using them, the criteria for selecting them, and the resources for obtaining them. A discussion of the various types of assistive devices and their application is followed by a review of the principles for selection and fitting and concludes with a review of the modern role of the hearing health care provider. C1 VA PUGET SOUND HLTH CARE SYST,SEATTLE,WA. MADIGAN ARMY MED CTR,DEPT OTOLARYNGOL HEAD & NECK SURG,TACOMA,WA 98431. DARREL L TETER & ASSOCIATES INC,HIGHLANDS RANCH,CO. NR 38 TC 5 Z9 5 U1 1 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0030-6665 J9 OTOLARYNG CLIN N AM JI Otolaryngol. Clin. N. Am. PD OCT PY 1997 VL 30 IS 5 BP 803 EP & PG 46 WC Otorhinolaryngology SC Otorhinolaryngology GA YB166 UT WOS:A1997YB16600010 PM 9295255 ER PT J AU Eibling, DE Boyd, EM AF Eibling, DE Boyd, EM TI Rehabilitation of lower cranial nerve deficits SO OTOLARYNGOLOGIC CLINICS OF NORTH AMERICA LA English DT Article ID VOCAL CORD PARALYSIS; RADICAL NECK DISSECTION; PASSY-MUIR VALVE; INTRACTABLE ASPIRATION; TRACHEOSTOMY; THYROPLASTY; DYSPHAGIA; INJECTION; PRESSURE; SURGERY AB Lower cranial nerve deficits following skull base surgery can be accompanied by significant morbidity, especially if the vagus nerve has been sacrificed or injured. Loss of pharyngeal function and glottic closure can result in dysphagia and aspiration. Left untreated, these can result in the major morbidity for the patient following skull base surgery. The authors discuss the management of lower cranial nerve deficits, with emphasis on rehabilitation of swallowing function and prevention of aspiration following vagal injury. C1 UNIV PITTSBURGH,SCH MED,VA PITTSBURGH HLTH CARE SYST,DEPT OTOLARYNGOL,PITTSBURGH,PA. WILFORD HALL USAF MED CTR,DEPT OTOLARYNGOL,LACKLAND AFB,TX 78236. NR 32 TC 7 Z9 7 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0030-6665 J9 OTOLARYNG CLIN N AM JI Otolaryngol. Clin. N. Am. PD OCT PY 1997 VL 30 IS 5 BP 865 EP & PG 12 WC Otorhinolaryngology SC Otorhinolaryngology GA YB166 UT WOS:A1997YB16600012 PM 9295257 ER PT J AU Meyer, JH Lake, R AF Meyer, JH Lake, R TI Mismatch of duodenal deliveries of dietary fat and pancreatin from enterically coated microspheres SO PANCREAS LA English DT Article DE pancreatic insufficiency; steatorrhea; treatment ID CYSTIC-FIBROSIS; ENZYME PREPARATION; CANINE MODEL; SOLID-LIQUID; INSUFFICIENCY; ABSORPTION; LIPASE; MEALS AB Gastric emptying of dietary fat is affected by both chemical and physical factors; but when ingested as a free oil or an aqueous emulsion, fat may empty most rapidly immediately after the meal. In contrast, gastric transit of 1- to 3-mm spheres (like those of enterically coated pancreatins) is known to vary inversely with sphere diameter; and spheres leave the stomach initially slowly, if their diameter is greater than or equal to 1.6 mm. Our objective was to determine whether 2-mm microspheres of Pancrease would empty much more slowly than free or emulsified oil and whether 1.2-mm microspheres of Creon would empty as fast as free oil. We used a gamma camera to track the concurrent gastric emptying of I-123-labeled oil and In-113m-labeled spheres of Pancrease or Creon in pancreatic-insufficient subjects with cystic fibrosis who ingested 20 g of free oil in spaghetti meals or 20 g of oil emulsified in a milk meal. We found that either type of oil emptied rapidly initially but slowed later, whereas either dosage form emptied slowly initially but rapidly later. Unexpectedly, the smaller spheres of Creon emptied about the same as Pancrease did after the spaghetti meal. For example, 50% of oil but <25% of either dosage form had left the stomach by 90 min after the meals. Both dosage forms were lipophilic, forming aggregates in vitro. We concluded that the gastric emptying of either dosage form frequently lagged behind the emptying of oil from ordinary meals. We speculated that the similar transits of the 1.2-mm Creon and the 2-mm Pancrease resulted from aggregation of these microspheres in the presence of free oil. C1 UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. W LOS ANGELES VET AFFAIRS MED CTR,DEPT MED,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,DEPT NUCL MED,LOS ANGELES,CA 90073. SEPULVEDA VA MED CTR,DEPT MED,LOS ANGELES,CA. SEPULVEDA VA MED CTR,DEPT NUCL MED,LOS ANGELES,CA. NR 28 TC 19 Z9 19 U1 0 U2 3 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0885-3177 J9 PANCREAS JI Pancreas PD OCT PY 1997 VL 15 IS 3 BP 226 EP 235 DI 10.1097/00006676-199710000-00003 PG 10 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA YJ775 UT WOS:A1997YJ77500003 PM 9336785 ER PT J AU Morrow, NS Schall, M Grijalva, CV Geiselman, PJ Garrick, T Nuccion, S Novin, D AF Morrow, NS Schall, M Grijalva, CV Geiselman, PJ Garrick, T Nuccion, S Novin, D TI Body temperature and wheel running predict survival times in rats exposed to activity-stress SO PHYSIOLOGY & BEHAVIOR LA English DT Article DE activity-stress; body temperature; wheel running; anorexia nervosa ID ACTIVITY-BASED ANOREXIA; ULCER AB The relationship between restricted feeding, core body temperature (T-b), wheel running, survival, and gastric erosion formation was examined in female rats exposed to activity-stress. Core body temperature and gross motor activity were telemetrically monitored in four groups of rats that had free access to running wheels and in one group that was not allowed to run on the wheels. Twenty-four hours prior to the onset of hypothermia and predicted mortality, different groups were left undisturbed, warmed with a heal lamp, denied access to running wheels, or euthanized. Length of survival in wheel-running rats varied from 2 to 12 days. During the first day of food deprivation, premorbid changes in the variability of T-b during the diurnal period and the mean number of wheel revolutions during the nocturnal period were strongly predictive of length of survival. Warming rats with a heat lamp or preventing rats from ever running on the wheel increased the length of survival and attenuated gastric erosion formation. only rats that were warmed had a greater likelihood of survival. Gastric pathology was also reduced in rats that were euthanized prior to becoming moribund. Rats that were left undisturbed or locked from the running wheel over the last 24 h of testing became moribund and had extensive gastric mucosal damage. These results indicate that thermoregulatory disturbances induced by restricted feeding and not wheel running alone are critical in determining survival and the degree of gastric mucosal injury in rats exposed to activity-stress. Results further suggest that predisposing factors may put some rats at risk for the development of activity-stress-induced mortality. (C) 1997 Elsevier Science Inc. C1 UNIV CALIF LOS ANGELES,DEPT PSYCHOL,LOS ANGELES,CA 90095. UNIV CALIF LOS ANGELES,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90095. UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,CURE,DIGEST DIS RES CTR,LOS ANGELES,CA 90073. TULANE UNIV,DEPT PSYCHOL,NEW ORLEANS,LA 70118. LOUISIANA STATE UNIV,DEPT PSYCHOL,BATON ROUGE,LA 70808. LOUISIANA STATE UNIV,PENNINGTON BIOMED RES CTR,BATON ROUGE,LA 70808. FU NIDDK NIH HHS [DK 41301, K04DK01897]; PHS HHS [5T32M17140] NR 26 TC 24 Z9 24 U1 0 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0031-9384 J9 PHYSIOL BEHAV JI Physiol. Behav. PD OCT PY 1997 VL 62 IS 4 BP 815 EP 825 DI 10.1016/S0031-9384(97)00243-6 PG 11 WC Psychology, Biological; Behavioral Sciences SC Psychology; Behavioral Sciences GA XT845 UT WOS:A1997XT84500020 PM 9284503 ER PT J AU Melany, ML Grant, EG Duerinckx, AJ Watts, TM Levine, BS AF Melany, ML Grant, EG Duerinckx, AJ Watts, TM Levine, BS TI Ability of a phase shift US contrast agent to improve imaging of the main renal arteries SO RADIOLOGY LA English DT Article DE ultrasound (US), contrast media; ultrasound (US), Doppler studies; hypertension, renovascular; renal arteries, stenosis or obstruction ID DOWNSTREAM CIRCULATION; SLOWED SYSTOLE; STENOSIS; DIAGNOSIS; TARDUS; ANGIOGRAPHY; ENHANCEMENT; SONOGRAPHY; PATTERN AB PURPOSE: To evaluate the ability of a phase shift contrast agent to improve Doppler sonographic imaging of the main renal arteries in cases of suspected renal artery stenosis. MATERIALS AND METHODS: In 25 patients in whom renal artery stenosis was suspected, baseline Doppler sonography was performed followed by two studies performed after blinded administration of contrast material or placebo (saline). Each kidney (n = 45) was evaluated for (n) visualization of the main renal artery on a scale of 0-4, (b) presence of accessory renal arteries, and (c) direct Doppler sonographic findings suggestive of renal artery stenosis. Correlative magnetic resonance angiography was performed in 24 patients, and angiograms were obtained in eight kidneys with stenosis at one or both imaging studies. RESULTS: Contrast enhancement was observed in 23 patients. Enhancement was 8-20 minutes. Renal artery visualization scores improved from a mean of 2.56 and 2.71 on baseline and noncontrast scans, respectively, to 3.69 after administration of contrast material. Contrast-enhanced images depicted seven kidneys with accessory renal arteries not seen at other studies. Two of eight cases of stenosis were seen only with contrast-enhanced sonography. CONCLUSION: Use of the phase shift contrast agent appears to enable a reduction in the number of equivocal findings of renal artery stenosis. C1 UNIV CALIF LOS ANGELES,DEPT INTERNAL MED,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,DEPT RADIOL,LOS ANGELES,CA. W LOS ANGELES VET AFFAIRS MED CTR,DEPT INTERNAL MED,LOS ANGELES,CA. RP Melany, ML (reprint author), UNIV CALIF LOS ANGELES,DEPT RADIOL SCI,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 22 TC 29 Z9 34 U1 1 U2 1 PU RADIOLOGICAL SOC NORTH AMER PI EASTON PA 20TH AND NORTHAMPTON STS, EASTON, PA 18042 SN 0033-8419 J9 RADIOLOGY JI Radiology PD OCT PY 1997 VL 205 IS 1 BP 147 EP 152 PG 6 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA XX266 UT WOS:A1997XX26600026 PM 9314976 ER PT J AU Freston, JW Ahnen, DJ Czinn, SJ Earnest, DL Farthing, MJ Gorbach, SL Hunt, RH Sandler, RS Schuster, MM AF Freston, JW Ahnen, DJ Czinn, SJ Earnest, DL Farthing, MJ Gorbach, SL Hunt, RH Sandler, RS Schuster, MM TI Review and analysis of the effects of Olestra, a dietary fat substitute, on gastrointestinal function and symptoms SO REGULATORY TOXICOLOGY AND PHARMACOLOGY LA English DT Review ID SORBITOL INTOLERANCE; NONABSORBABLE LIPIDS; SUCROSE POLYESTER; PREVALENCE; DISEASE; ENERGY; HEALTH; ABSORPTION; ADULTS; RAT AB Olestra, a dietary fat substitute, was recently made available to consumers in savory snacks in three cities. Early reports of gastrointestinal complaints attributed to olestra attracted media coverage and fostered confusion among physicians and consumers about the nature of olestra and its effects on the digestive system, We reviewed all published studies of olestra's gastrointestinal effects and all relevant unpublished studies submitted to the Food and Drug Administration. Each study was analyzed by a group of expert gastroenterologists and epidemiologists. The symptoms reported with olestra ingestion are similar to those reported with ingestion of fiber and sorbitol, although the mechanisms involved in changing stool characteristics differ among these food additives. Olestra's effects on stool habit and characteristics are due to its presence in the stool. Large amounts are more likely to induce gastrointestinal symptoms than small amounts. There is no evidence that olestra induces pathological change in bowel function: there is no increased fluid or electrolyte nor is there altered gastrointestinal motility or microflora, Olestra and triglyceride ingestion resulted in a similar frequency of symptoms in normal adults and children and in people with chronic inflammatory bowel disease in remission, Olestra traverses the digestive tract intact to become a stool additive. Some subjects develop a change in bowel habit and stool characteristics due to the presence of more olestra in the stool, These changes resemble those associated with ingestion of sorbitol and fiber. (C) 1997 Academic Press. C1 UNIV COLORADO,SCH MED,DENVER,CO. DENVER VA MED CTR,DEPT MED,DENVER,CO. CASE WESTERN RESERVE UNIV,SCH MED,DEPT PEDIAT,CLEVELAND,OH 44106. UNIV ARIZONA,HLTH SCI CTR,DEPT MED,TUCSON,AZ 85721. ST BARTHOLOMEWS & ROYAL LONDON SCH MED & DENT,DIGEST DIS RES CTR,LONDON,ENGLAND. TUFTS UNIV,SCH MED,DEPT COMMUNITY HLTH,BOSTON,MA 02111. TUFTS UNIV,SCH MED,DEPT MED,BOSTON,MA 02111. MCMASTER UNIV,DEPT MED,HAMILTON,ON L8S 4L8,CANADA. UNIV N CAROLINA,DEPT MED,CHAPEL HILL,NC 27515. JOHNS HOPKINS UNIV,BAYVIEW MED CTR,DEPT MED,BALTIMORE,MD 21218. RP Freston, JW (reprint author), UNIV CONNECTICUT,CTR HLTH,DEPT MED,FARMINGTON,CT 06032, USA. NR 36 TC 17 Z9 17 U1 0 U2 4 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0273-2300 J9 REGUL TOXICOL PHARM JI Regul. Toxicol. Pharmacol. PD OCT PY 1997 VL 26 IS 2 BP 210 EP 218 DI 10.1006/rtph.1997.1165 PG 9 WC Medicine, Legal; Pharmacology & Pharmacy; Toxicology SC Legal Medicine; Pharmacology & Pharmacy; Toxicology GA YH056 UT WOS:A1997YH05600008 PM 9356284 ER PT J AU Badr, MS Kawak, A Skatrud, JB Morrell, MJ Zahn, BR Babcock, MA AF Badr, MS Kawak, A Skatrud, JB Morrell, MJ Zahn, BR Babcock, MA TI Effect of induced hypocapnic hypopnea on upper airway patency in humans during NREM sleep SO RESPIRATION PHYSIOLOGY LA English DT Article DE control of breathing, reduced ventilatory drive; mammals, humans; sleep, upper airway patency; upper airways, patency, hypopnea ID TOTAL PULMONARY RESISTANCE; HYPOXIA; OBSTRUCTION; APNEA AB We wished to determine the effect of reduced ventilatory drive (hypopnea) on upper airway patency in humans during non-rapid-eye-movement (NREM) sleep. We studied nine subjects (58 trials) spanning the spectrum of susceptibility to upper airway collapse including normals, snorers and patients with mild sleep apnea. Hypocapnic hypopnea was induced by abrupt cessation of brief (1 min) nasal mechanical hyperventilation. Surface inspiratory EMG (EMG(insp)) was used as an index of drive. Upper airway resistance and supraglottic pressure-flow plots were used as indexes of upper airway patency. Termination of nasal mechanical ventilation resulted in reduced (V) over dot E to 49% of pre-mechanical ventilation eupneic control. Upper airway resistance at a fixed flow did not change significantly in inspiration or expiration. Likewise, pressure-flow plots showed no increase in upper airway resistance except in one subject. However, maximum flow ((V) over dot max) decreased during hypopnea in four subjects who demonstrated inspiratory flow-limitation (IFL) during eupneic control. in contrast, no IFL was noted in subjects who showed no evidence of IFL during eupnea. We concluded: (1) Reduced ventilatory drive does not compromise upper airway patency in normal subjects during NREM sleep; (2) the reduction in (V) over dot max during hypopnea in subjects with IFL during eupneic control, suggests that reduced drive is associated with increased upper airway compliance in these subjects; and (3) upper airway susceptibility to narrowing/closure is an important determinant of the response to induced hypopnea during NREM sleep. (C) 1997 Elsevier Science B.V. C1 UNIV WISCONSIN,SCH MED,WILLIAM S MIDDLETON MEM VET HOSP,MADISON,WI. UNIV WISCONSIN,SCH MED,DEPT MED,MADISON,WI. UNIV WISCONSIN,SCH MED,DEPT PREVENT MED,MADISON,WI. WAYNE STATE UNIV,SCH MED,DEPT MED,DETROIT,MI 48201. RP Badr, MS (reprint author), DETROIT VA MED CTR,4646 JOHN R,DETROIT,MI 48201, USA. FU NHLBI NIH HHS [HL-07654]; PHS HHS [R29 53443, P01 42242] NR 15 TC 34 Z9 34 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0034-5687 J9 RESP PHYSIOL JI Respir. Physiol. PD OCT PY 1997 VL 110 IS 1 BP 33 EP 45 DI 10.1016/S0034-5687(97)00072-8 PG 13 WC Physiology; Respiratory System SC Physiology; Respiratory System GA YD149 UT WOS:A1997YD14900004 PM 9361150 ER PT J AU Sakaguchi, N Yoshimura, M Hershman, JM Nishikawa, M Inada, M AF Sakaguchi, N Yoshimura, M Hershman, JM Nishikawa, M Inada, M TI Paracrine effect of human chorionic gonadotropin ectopically produced from papillary thyroid cancer cells on growth and function of FRTL-5 rat thyroid cells SO THYROID LA English DT Article ID HUMAN TSH RECEPTORS; CARCINOMA; HORMONE AB It is well known that human chorionic gonadotropin (hCG) is sometimes secreted from nontrophoblastic neoplasms. To elucidate the role of ectopic hCG, we investigated the effect of hCG produced from a papillary thyroid cancer cell line (B-CPAP cells) on stimulation and growth promotion of FRTL-5 rat thyroid cells. Ectopic hCG contained in the culture medium of B-CPAP cells was purified using gel filtration and bioassayed for thyrotropic activity in FRTL-5 cells. Addition of ectopic hCG (up to 5.2 x 10(4) IU/L) increased cyclic adenosine monophosphate (cAMP) accumulation and H-3-thymidine incorporation in FRTL-5 cells dose dependently. These effects were almost as potent as the stimulation induced by standard hCG CR-127. After the absorption of the ectopic hCG by anti-hCG-beta monoclonal antibody, the cAMP accumulation was significantly decreased. Analysis of ectopic hCG isoforms with different isoelectric points indicated the predominance of the acidic hCG isoform with isoelectric point (pI) 3.8-3.2 that is the major isoform of standard hCG. Basic isoforms (pI 5.7-5.3) with higher thyrotropic potency were also detected. These results indicate that the ectopic hCG secreted from papillary thyroid cancer cells possess intrinsic thyroid-stimulating and growth-promoting activity. The ectopic hCG may act as an autocrine-paracrine factor in nontrophoblastic neoplasms. C1 W LOS ANGELES VET AFFAIRS MED CTR, DIV ENDOCRINOL & METAB, LOS ANGELES, CA 90073 USA. RP Sakaguchi, N (reprint author), KANSAI MED UNIV, DEPT INTERNAL MED 2, HEMODIALYSIS CTR, 10-15 FUMIZONOCHO, MORIGUCHI, OSAKA 570, JAPAN. NR 19 TC 3 Z9 4 U1 0 U2 0 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1050-7256 EI 1557-9077 J9 THYROID JI Thyroid PD OCT PY 1997 VL 7 IS 5 BP 779 EP 782 DI 10.1089/thy.1997.7.779 PG 4 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA YD729 UT WOS:A1997YD72900014 PM 9349584 ER PT J AU Moon, TD AF Moon, TD TI Questionnaire survey of urologists and primary care physicians' diagnostic and treatment practices for prostatitis SO UROLOGY LA English DT Article ID PROSTATODYNIA AB Objectives, To establish diagnostic and treatment practices for chronic prostatitis by survey of urologists in Wisconsin and primary care providers in Dane County, Wisconsin. Methods. All Wisconsin urologists (n = 135) and primary care providers in Dane County, Wisconsin (n = 365) were surveyed by mail with a 10-item questionnaire used to establish diagnostic and treatment practices for prostatitis. Results. Seventy-eight percent of primary caregivers consider prostatitis to be bacterial in nature, whereas 59% of urologists consider it to be noninfectious. Fewer than 50% of primary care providers consider pain to be other than perineal in the diagnosis. Fewer than 50% of urologists or primary caregivers evaluate expressed prostatic secretions and few primary care providers (11%) use nonantibiotic therapy. Conclusions. The diagnostic and treatment practices for prostatitis do not follow standard textbook algorithms. C1 UNIV WISCONSIN HOSP & CLIN,WILLIAM S MIDDLETON MEM VET ADM HOSP,SURG SERV,MADISON,WI 53792. RP Moon, TD (reprint author), UNIV WISCONSIN,DIV UROL,CLIN SCI CTR G5 341,DEPT SURG,600 HIGHLAND AVE,MADISON,WI 53792, USA. NR 14 TC 67 Z9 73 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0090-4295 J9 UROLOGY JI UROLOGY PD OCT PY 1997 VL 50 IS 4 BP 543 EP 547 DI 10.1016/S0090-4295(97)00308-7 PG 5 WC Urology & Nephrology SC Urology & Nephrology GA YB077 UT WOS:A1997YB07700012 PM 9338729 ER PT J AU Buchner, DM AF Buchner, DM TI Preserving mobility in older adults SO WESTERN JOURNAL OF MEDICINE LA English DT Article ID RANDOMIZED CONTROLLED TRIAL; HUMAN GROWTH-HORMONE; LEG EXTENSOR POWER; MUSCLE STRENGTH; PHYSICAL-ACTIVITY; RISK-FACTORS; ALCOHOL-CONSUMPTION; REPLACEMENT THERAPY; FUNCTIONAL ABILITY; BODY-COMPOSITION AB Age-related loss of strength contributes to impaired mobility and increases the risk of falls. Recent research has focused on 2 approaches to preventing age-related loss of strength-promoting physical activity and exercise (especially strength training) and using trophic factors to enhance muscle performance. Epidemiologic evidence strongly supports a role of regular physical activity in successful aging by preserving muscle performance, promoting mobility, and reducing fall risk. Randomized controlled trials provide convincing evidence that strength and endurance training improve muscle performance in older adults. Evidence is rapidly accumulating from randomized trials that endurance, strength, and balance training promote mobility and reduce fall risk, though exercise effects differ according to the type of exercise, details of the exercise program, and the target group of older adults. Because lifetime regular physical activity is recommended for all older adults, a reasonable strategy (especially for weak adults) is an activity program that includes strength training. In contrast, insufficient evidence exists to recommend the long-term use of trophic factors to preserve muscular performance. An intervention that merits additional study is avoiding the use of psychoactive drugs because drugs like benzodiazepines appear to be risk factors for inactivity and may have unrecognized direct effects on muscular performance. Because chronic illness is a risk factor for inactivity and disuse muscle atrophy, randomized trials comparing strength training with other interventions would be useful in understanding whether-strength training has advantages in preserving muscle performance and improving health-related quality of life in a variety of chronic illnesses such as depressive illness. C1 UNIV WASHINGTON,SCH MED,NW PREVENT EFFECTIVENESS CTR,SEATTLE,WA 98195. VET AFFAIRS PUGENT SOUND HLTH CARE SYST,NW CTR OUTCOMES RES OLDER ADULTS,SEATTLE,WA. RP Buchner, DM (reprint author), UNIV WASHINGTON,SCH MED,DEPT HLTH SERV,BOX 358852,SEATTLE,WA 98195, USA. FU NIA NIH HHS [UO1/AG09095]; PHS HHS [R48/CCR002181] NR 93 TC 32 Z9 32 U1 2 U2 3 PU CARDEN JENNINGS PUBL CO LTD PI CHARLOTTESVILLE PA BLAKE CTR, STE 200, 1224 W MAIN ST, CHARLOTTESVILLE, VA 22903 SN 0093-0415 J9 WESTERN J MED JI West. J. Med. PD OCT PY 1997 VL 167 IS 4 BP 258 EP 264 PG 7 WC Medicine, General & Internal SC General & Internal Medicine GA YB464 UT WOS:A1997YB46400011 PM 9348757 ER PT J AU Huang, Z Cabanela, V Howell, T AF Huang, Z Cabanela, V Howell, T TI Stress, bottlefeeding, and diabetes SO LANCET LA English DT Letter C1 UNIV WISCONSIN,WISCONSIN REG PRIMATE RES CTR,DEPT AGING & METAB DIS,SECT GERIATR & GERONTOL,MADISON,WI. UNIV WISCONSIN,DEPT MED,MADISON,WI. UNIV WISCONSIN,DEPT PSYCHIAT,MADISON,WI 53706. RP Huang, Z (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON GERIAT RES EDUC & CLIN CTR,2500 OVERLOOK TERRACE,MADISON,WI 53705, USA. NR 5 TC 3 Z9 3 U1 0 U2 2 PU LANCET LTD PI LONDON PA 42 BEDFORD SQUARE, LONDON, ENGLAND WC1B 3SL SN 0140-6736 J9 LANCET JI Lancet PD SEP 20 PY 1997 VL 350 IS 9081 BP 889 EP 889 DI 10.1016/S0140-6736(05)62075-2 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA XX401 UT WOS:A1997XX40100065 PM 9310631 ER PT J AU Gelernter, J VanDyck, C vanKammen, DP Malison, R Price, LH Cubells, JF Berman, R Charney, DS Heninger, G AF Gelernter, J VanDyck, C vanKammen, DP Malison, R Price, LH Cubells, JF Berman, R Charney, DS Heninger, G TI Ciliary neurotrophic factor null allele frequencies in schizophrenia, affective disorders, and Alzheimer's disease SO AMERICAN JOURNAL OF MEDICAL GENETICS LA English DT Article DE CNTF; genetic association; schizophrenia; affective disorders; Alzheimer's disease; population stratification; null alleles ID CNTF GENE; RECEPTOR GENE; ASSOCIATION; MUTATION AB Ciliary neurotrophic factor (CNTF) is a cytokine that has been reported to affect cellular differentiation. Mouse CNTF knockouts have progressive motor neuron atrophy, but this protein has uncertain physiological function in humans, A naturally occurring CNTF variant in man, observed in many populations, abolishes function of the protein product, providing the opportunity to study loss of CNTF function in humans, It has been reported previously that this variant does not predispose to several neurological and neuropsychiatric disorders, including Alzheimer's disease, but findings have been more ambiguous with respect to other conditions, such as schizophrenia, We report here allele frequencies for this null mutation in populations diagnosed with mood disorders (unipolar depression, single episode or recurrent; N = 59), schizophrenia (N = 66), or Alzheimer's disease (N = 93), We found no association of the CNTF null with any of these phenotypes, There is presently no known phenotype consistently associated with either heterozygosity or homozygosity for the CNTF null allele, suggesting either that this protein does not serve a necessary function in humans or is redundant with some other protein or any human phenotype associated with absence of CNTF is considerably more subtle than that seen in mouse, (C) 1997 Wiley-Liss, Inc. C1 YALE UNIV,SCH MED,DIV MOL PSYCHIAT,DEPT PSYCHIAT,NEW HAVEN,CT. VA PITTSBURGH HLTH CARE SYST,PITTSBURGH,PA. WESTERN PSYCHIAT INST & CLIN,PITTSBURGH,PA. BUTLER HOSP,PROVIDENCE,RI 02906. BROWN UNIV,SCH MED,DEPT PSYCHIAT & HUMAN BEHAV,PROVIDENCE,RI 02912. RP Gelernter, J (reprint author), VA CONNECTICUT HEALTHCARE SYST,DEPT PSYCHIAT,PSYCHIAT 116A2,W HAVEN CAMPUS,950 CAMPBELL AVE,W HAVEN,CT 06516, USA. FU NIMH NIH HHS [MH44841, MH01387, MH00931] NR 22 TC 15 Z9 15 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0148-7299 J9 AM J MED GENET JI Am. J. Med. Genet. PD SEP 19 PY 1997 VL 74 IS 5 BP 497 EP 500 DI 10.1002/(SICI)1096-8628(19970919)74:5<497::AID-AJMG8>3.0.CO;2-L PG 4 WC Genetics & Heredity SC Genetics & Heredity GA YA947 UT WOS:A1997YA94700008 PM 9342199 ER PT J AU Ramakrishnan, S Sharma, HW Farris, AD Kaufman, KM Harley, JB Collins, K Pruijn, GJM vanVenrooij, WJ Martin, ML Narayanan, R AF Ramakrishnan, S Sharma, HW Farris, AD Kaufman, KM Harley, JB Collins, K Pruijn, GJM vanVenrooij, WJ Martin, ML Narayanan, R TI Characterization of human telomerase complex SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE bioinformatics; Ro60; RNP; autoantigen; telomeres ID HUMAN RO RIBONUCLEOPROTEIN; TETRAHYMENA TELOMERASE; SECONDARY STRUCTURE; SJOGRENS-SYNDROME; IMMORTAL CELLS; AUTO-ANTIGEN; AUTOANTIBODIES; ANTI-LA(SS-B); PURIFICATION; ASSOCIATION AB Telomerase, a ribonucleoprotein complex, adds hexameric repeats called ''telomeres'' to the growing ends of chromosomal DNA. Characterization of mammalian telomerase has been elusive because of its low level of expression, We describe a bioinformatics approach to enrich and characterize the human telomerase complex. Using local sequence homology search methods, we detected similarity of the Tetrahymena p80 subunit of telomerase with the autoantigen Ro60, Antibodies to Ro60 immunoprecipitated the telomerase activity, Ro60 and p80 proteins were cross-recognizable by antibodies to either protein, Telomerase activity and the RNA component of telomerase complex were localized to a doublet in a native gel from the Ro60 antibody-precipitated material, The enriched material showed specific binding to a TTA GGG probe in vitro in an RNA template-dependent manner, Polyclonal antibodies to the doublet also immunoprecipitated the telomerase activity. These results suggest an evolutionary conservation of the telomerase proteins. C1 HOFFMANN LA ROCHE INC,ROCHE RES CTR,DEPT ONCOL,NUTLEY,NJ 07110. HOFFMANN LA ROCHE INC,ROCHE RES CTR,DEPT BIOINFORMAT,NUTLEY,NJ 07110. US DEPT VET AFFAIRS,OKLAHOMA MED RES FDN,OKLAHOMA CITY,OK 73104. UNIV OKLAHOMA,HLTH SCI CTR,OKLAHOMA CITY,OK 73104. UNIV CALIF BERKELEY,DEPT MOL & CELL BIOL,BERKELEY,CA 94720. UNIV NIJMEGEN,DEPT BIOCHEM,NL-6500 HB NIJMEGEN,NETHERLANDS. RI Pruijn, Ger/D-6664-2012 OI Narayanan, Ramaswamy/0000-0001-9346-9083 NR 27 TC 22 Z9 22 U1 1 U2 5 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD SEP 16 PY 1997 VL 94 IS 19 BP 10075 EP 10079 DI 10.1073/pnas.94.19.10075 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA XX399 UT WOS:A1997XX39900015 PM 9294165 ER PT J AU Barrachina, MD Martinez, V Wang, LX Wei, JY Tache, Y AF Barrachina, MD Martinez, V Wang, LX Wei, JY Tache, Y TI Synergistic interaction between leptin and cholecystokinin to reduce short-term food intake in lean mice SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID C-FOS EXPRESSION; DORSAL VAGAL COMPLEX; RAT VAGUS NERVE; OB PROTEIN; PARAVENTRICULAR NUCLEUS; BINDING-SITES; OB/OB MICE; CIRCULATING CHOLECYSTOKININ; ENDOGENOUS CHOLECYSTOKININ; CCK-ANTAGONISTS AB Leptin is a circulating protein involved in the long-term regulation of food intake and body weight. Cholecystokinin (CCK) is released postprandially and elicits satiety signals. We investigated the interaction between leptin and CCK-8 in the short-term regulation of food intake induced by 24-hr fasting in lean mice. Leptin, injected intraperitoneally (i.p.) at low doses (4-120 mu g/kg), which did not influence feeding behavior for the first 3 hr postinjection, decreased food intake dose dependently by 47-83% during the first hour when coinjected with a subthreshold dose of CCK. Such an interaction was not observed between leptin and bombesin. The food-reducing effect of leptin injected with CCK was not associated with alterations in gastric emptying or locomotor behavior. Leptin-CCK action was blocked by systemic capsaicin at a dose inducing functional ablation of sensory afferent fibers and by devazepide, a CCK-A receptor antagonist but not by the CCK-B receptor antagonist, L-365,260. The decrease in food intake which occurs 5 hr after i.p. injection of leptin alone was also blunted by devazepide. Coinjection of leptin and CCK enhanced the number of Fos-positive cells in the hypothalamic paraventricular nucleus by 60%, whereas leptin or CCK alone did not modify Fos expression. These results indicate the existence of a functional synergistic interaction between leptin and CCK leading to early suppression of food intake which involves CCK-A receptors and capsaicin-sensitive afferent fibers. C1 W LOS ANGELES VET AFFAIRS MED CTR,CURE DIGEST DIS RES CTR,DEPT MED,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,BRAIN RES INST,LOS ANGELES,CA 90024. RI Martinez, Vicente/N-1189-2014 FU NIDDK NIH HHS [DK 41301, DK 30110, P30 DK041301]; NIMH NIH HHS [MH 00663] NR 67 TC 328 Z9 330 U1 0 U2 4 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD SEP 16 PY 1997 VL 94 IS 19 BP 10455 EP 10460 DI 10.1073/pnas.94.19.10455 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA XX399 UT WOS:A1997XX39900082 PM 9294232 ER PT J AU Kowluru, A Li, GD Metz, SA AF Kowluru, A Li, GD Metz, SA TI Glucose activates the carboxyl methylation of gamma subunits of trimeric GTP-binding proteins in pancreatic beta cells - Modulation in vivo by calcium, GTP, and pertussis toxin SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article; Proceedings Paper CT 16th International-Diabetes-Federation Congress CY JUL 20-25, 1997 CL HELSINKI, FINLAND SP Int Diabet Federat DE pancreatic beta cells; GTP-binding proteins; subunit carboxyl methylation; prostaglandin E-2; cyclooxygenase inhibitors and insulin secretion ID INDUCED INSULIN-SECRETION; PHOSPHOLIPASE-C; NORMAL RAT; FUNCTIONAL-SIGNIFICANCE; REGULATORY PROTEINS; ARACHIDONIC-ACID; ALPHA-SUBUNIT; KINASE-C; ISLETS; RECEPTOR AB The gamma subunits of trimeric G-proteins (gamma 1, gamma 1, gamma(5), and gamma(7) isoforms) were found to be methylated at their carboxyl termini in normal rat islets, human islets and pure beta [HIT-T15] cells. Of these, GTP gamma S significantly stimulated the carboxyl methylation selectively of gamma(2) and gamma(5) isoforms. Exposure of intact HIT cells to either of two receptor-independent agonists-a stimulatory concentration of glucose or a depolarizing concentration of K+-resulted in a rapid (within 30 s) and sustained (at least up to 60 min) stimulation of gamma subunit carboxyl methylation. Mastoparan, which directly activates G-proteins (and insulin secretion from beta cells), also stimulated the carboxyl methylation of gamma subunits in intact HIT cells. Stimulatory effects of glucose or K+ were not demonstrable after removal of extracellular Ca2+ or depletion of intracellular GTP, implying regulatory roles for calcium fluxes and GTP; however, the methyl transferase itself was not directly activated by either, The stimulatory effects of mastoparan were resistant to removal of extracellular Ca2+, implying a mechanism of action that is different from glucose or K+ but also suggesting that dissociation of the alpha beta gamma trimer is conducive to gamma subunit carboxyl methylation. Indeed, pertussis toxin also markedly attenuated the stimulatory effects of glucose, K+ or mastoparan without altering the rise in intracellular calcium induced by glucose or K+, Glucose-induced carboxyl methylation of gamma(2) and gamma(5) isoforms was vitiated by coprovision of any of three structurally different cyclooxygenase inhibitors. Conversely, exogenous PGE(2), which activates G(i) and G(o) in HIT cells and which thereby would dissociate alpha from beta(gamma), stimulated the carboxyl methylation of gamma(2) and gamma(5) isoforms and reversed the inhibition of glucose-stimulated carboxyl methylation of gamma subunits elicited by cyclooxygenase inhibitors. These data indicate that gamma subunits of trimeric G-proteins undergo a glucose-and calcium-regulated methylation-demethylation cycle in insulin-secreting cells, findings that may imply an important role in beta cell function. Furthermore, this is the first example of the regulation of the posttranslational modification of G-protein gamma subunits vi a nonreceptor-mediated activation mechanisms, which are apparently dependent on calcium influx and the consequent activation of phospholipases releasing arachidonic acid. C1 UNIV WISCONSIN,DEPT MED,SCH MED,MADISON,WI 53792. WILLIAM S MIDDLETON MEM VET ADM MED CTR,RES SERV,MADISON,WI 53705. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MED SERV,MADISON,WI 53705. NATL UNIV SINGAPORE,INST MED,SINGAPORE 119260,SINGAPORE. RP Kowluru, A (reprint author), UNIV WISCONSIN,CTR CLIN SCI,SCH MED,DIV ENDOCRINOL,H4-568,600 HIGHLAND AVE,MADISON,WI 53792, USA. FU NIDDK NIH HHS [DK 37312] NR 79 TC 33 Z9 34 U1 0 U2 0 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD SEP 15 PY 1997 VL 100 IS 6 BP 1596 EP 1610 DI 10.1172/JCI119684 PG 15 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA XY730 UT WOS:A1997XY73000034 PM 9294129 ER PT J AU Kiraly, A Suto, G Guth, PH Tache, Y AF Kiraly, A Suto, G Guth, PH Tache, Y TI Ketotifen prevents gastric hyperemia induced by intracisternal thyrotropin-releasing hormone at a low dose SO EUROPEAN JOURNAL OF PHARMACOLOGY LA English DT Article DE acid secretion; gastric mucosal blood flow; ketotifen; mast cell; mean arterial pressure; RX 77368; TRH (thyrotropin-releasing hormone); vagus; brain-gut ID GENE-RELATED PEPTIDE; HYDROGEN GAS CLEARANCE; MUCOSAL BLOOD-FLOW; RAT MAST-CELLS; NERVOUS-SYSTEM; SUBSTANCE-P; SUGGESTIVE EVIDENCE; EFFERENT FUNCTION; ANESTHETIZED RAT; SENSORY NEURONS AB The thyrotropin-releasing hormone (TRH) analog, RX 77368, (p-Glu-His-(3,3'-dimethyl)-Pro-NH2) injected intracisternally (i.c.) at low doses increases gastric mucosal blood flow through vagal cholinergic and calcitonin gene-related peptide dependent pathways. The influence of the mast cell stabilizer, ketotifen, on i.c. injection of RX 77368 (1.5 ng)-induced changes in gastric mucosal blood flow (hydrogen gas-clearance technique), gastric acid secretion and mean arterial pressure was studied in urethane-anesthetized rats. RX 77368 increased gastric blood flow by 131% and systemic arterial pressure by 11 mm Hg and decreased gastric mucosal vascular resistance by 54% whereas acid secretion was not altered within the 30 min period post injection. Ketotifen had no effect on these basal parameters but abolished i.c. RX 77368-induced increased gastric mucosal blood flow and decreased gastric vascular resistance. These data suggest that mast cells may be part of the peripheral mechanisms involved in vagal gastric hyperemia induced by TRH analog injected i.c. at a low dose. (C) 1997 Elsevier Science B.V. C1 W LOS ANGELES VET AFFAIRS MED CTR,CURE,DIGEST DIS RES CTR,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,BRAIN RES INST,LOS ANGELES,CA 90073. FU NIDDK NIH HHS [DK-41301, DK-30110]; NIMH NIH HHS [MH-00663] NR 55 TC 5 Z9 5 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-2999 J9 EUR J PHARMACOL JI Eur. J. Pharmacol. PD SEP 10 PY 1997 VL 334 IS 2-3 BP 241 EP 247 DI 10.1016/S0014-2999(97)01186-2 PG 7 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA YC089 UT WOS:A1997YC08900015 PM 9369354 ER PT J AU Ellis, KK Oehlke, M Helfand, M Lieberman, D AF Ellis, KK Oehlke, M Helfand, M Lieberman, D TI Management of symptoms of gastroesophageal reflux disease: Does endoscopy influence medical management? SO AMERICAN JOURNAL OF GASTROENTEROLOGY LA English DT Article ID BENIGN ESOPHAGEAL STRICTURES; CONSERVATIVE TREATMENT; NATURAL-HISTORY; PREVALENCE; BOUGIENAGE; DILATATION AB Objectives: The purpose of this study was to examine the theories that underlie the clinical decision to perform endoscopy in patients with symptoms of gastroesophageal reflux disease (GERD). Physicians reported that they use endoscopic findings to modify medical treatment of GERD. This study was undertaken to test this hypothesis in clinical practice. Methods: A consortium of community specialists in gastrointestinal disease was formed. Physicians completed a database on patients undergoing elective endoscopy for symptoms of GERD, which includes symptom severity, endoscopic findings, and medical treatment before and after endoscopy. An increase in medical treatment was defined as an increase in acid suppression therapy, and/or the addition of a promotility drug, and/or referral for surgery. Results: Data were collected prospectively over 6 months on 664 patients with symptoms of GERD, and complete data were available on 598 patients. Barrett's esophagus or active esophagitis (erythema, erosions, or ulceration) was present in 374 patients. Of these patients, 74% had an increase in therapy after endoscopy; for only 5% did therapy decrease. In contrast, among 224 patients with a normal-appearing esophagus, 35% had an increase in treatment and 65% had either a decrease in treatment or no change. In most cases, the increase in treatment was due to persistence of symptoms or because of endoscopic findings in the stomach or duodenum. The differences in treatment changes between the two groups was highly significant (p < 0.0001). Conclusion: The results support the theory that physicians often use endoscopic results to tailor medical therapy in patients with symptoms of GERD. C1 PORTLAND VET AFFAIRS MED CTR,DEPT VET AFFAIRS,PORTLAND,OR 97207. OREGON HLTH SCI UNIV,DEPT MED,DIV GASTROENTEROL,PORTLAND,OR 97201. NR 16 TC 27 Z9 28 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-9270 J9 AM J GASTROENTEROL JI Am. J. Gastroenterol. PD SEP PY 1997 VL 92 IS 9 BP 1472 EP 1474 PG 3 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA XX187 UT WOS:A1997XX18700013 PM 9317065 ER PT J AU Tagliente, TM AF Tagliente, TM TI Pharmacoeconomics of propofol in anesthesia SO AMERICAN JOURNAL OF HEALTH-SYSTEM PHARMACY LA English DT Review DE anesthetics; costs; hospitals; nausea; patient care; pharmacoeconomics; propofol; toxicity; vomiting ID ISOFLURANE NITROUS-OXIDE; COST-EFFECTIVENESS ANALYSIS; INTENSIVE-CARE UNIT; CARDIAC-SURGERY; OUTPATIENT ANESTHESIA; POSTOPERATIVE NAUSEA; INTRAVENOUS ANESTHESIA; STRABISMUS SURGERY; GENERAL-ANESTHESIA; PATIENT CHARGES AB Pharmacoeconomic data on the use of propofol in anesthesia are reviewed, with consideration of clinical characteristics that affect overall costs. Propofol is more expensive than many other anesthetics but its use can affect the costs of perioperative care as well as costs not directly related to the health care system, such as those associated with time off work. Using propofol can result in earlier discharge from the postanesthesia care unit (PACU), which can result in lower PACU costs. Anesthesia induction and maintenance with propofol versus barbiturates for induction and volatile anesthetics for maintenance have been associated with a lower frequency of postoperative nausea and vomiting (PONV) in the immediate postoperative period. A reduced frequency of PONV may improve the ability to increase patient flow through the PACU, which may reduce PACU costs. The cost of treating PONV in the PACU might not be high enough to justify the use of more expensive agents such as propofol solely for their reduced propensity to cause PONV. Propofol use has been associated with significantly shorter times to extubation of cardiac-surgery patients compared with the use of other agents. Early extubation has, in turn, been associated with shorter hospital stays, shorter stays in the cardiac intensive care unit, and lower hospital charges. Policy changes regarding criteria for discharge from the PACU may need to be implemented if the economic advantages of propofol are to be realized. Anesthesia with propofol, compared with other agents, has been associated with shorter stays in the PACU. Whether using propofol decreases the costs of care is unclear. C1 MT SINAI SCH MED,NEW YORK,NY. RP Tagliente, TM (reprint author), BRONX VET AFFAIRS MED CTR,ANESTHESIOL SECT,BOX 112-A,130 W KINGSBRIDGE RD,BRONX,NY 10468, USA. NR 65 TC 10 Z9 11 U1 0 U2 1 PU AMER SOC HEALTH-SYSTEM PHARMACISTS PI BETHESDA PA 7272 WISCONSIN AVE, BETHESDA, MD 20814 SN 1079-2082 J9 AM J HEALTH-SYST PH JI Am. J. Health-Syst. Pharm. PD SEP 1 PY 1997 VL 54 IS 17 BP 1953 EP 1962 PG 10 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA XW723 UT WOS:A1997XW72300011 PM 9290892 ER PT J AU Spannagel, AW Reeve, JR Liddle, RA Guan, DF Green, GM AF Spannagel, AW Reeve, JR Liddle, RA Guan, DF Green, GM TI An amino-terminal fragment of LCRF, LCRF-(1-35), has the same activity as the natural peptide SO AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY LA English DT Article DE exocrine pancreas; negative feedback regulation; luminal cholecystokinin-releasing factor ID CHOLECYSTOKININ-RELEASING PEPTIDE; BASAL PANCREATIC-SECRETION; FEEDBACK-REGULATION; PLASMA CHOLECYSTOKININ; TRYPSIN-INHIBITOR; RAT; BILE; INTESTINE; PROTEIN; JUICE AB A cholecystokinin (CCK)-releasing peptide, luminal CCK-releasing factor (LCRF), has been purified from rat jejunal secretion. Amino acid analysis and mass spectral analysis showed that the purified peptide is composed of 70-75 amino acid residues and has a mass of 8,136 Da. Microsequence analysis of LCRF yielded an amino acid sequence for the amino-terminal 41 residues. To determine the biologically active region of the molecule, a peptide was synthesized consisting of the amino-terminal 35 amino acids of LCRF. In this study, intraduodenal infusion of LCRF-(1-35) significantly stimulated pancreatic secretion in conscious rats. The dose-response curves to LCRF-(1-35) and to monitor peptide were similar and biphasic, with higher doses producing submaximal pancreatic secretory responses. The CCK-A receptor antagonist MK-329 abolished the pancreatic secretory response to intraduodenally infused LCRF-(1-35). These results demonstrate that LCRF biological activity is contained within the amino-terminal 35-amino acid portion of LCRF, and this fragment may be useful for investigating the role of LCRF in gastrointestinal function. C1 UNIV TEXAS, HLTH SCI CTR, DEPT PHYSIOL, SAN ANTONIO, TX 78284 USA. UNIV CALIF LOS ANGELES, W LOS ANGELES VET AFFAIRS MED CTR, DEPT MED, DURHAM, NC 27710 USA. UNIV CALIF LOS ANGELES, W LOS ANGELES VET AFFAIRS MED CTR, CURE, DURHAM, NC 27710 USA. UNIV CALIF LOS ANGELES, W LOS ANGELES VET AFFAIRS MED CTR, DIGEST DIS RES CTR, DURHAM, NC 27710 USA. FU NIDDK NIH HHS [R01-DK-33850, DK-37482, DK-38626] NR 19 TC 7 Z9 7 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0193-1857 J9 AM J PHYSIOL-GASTR L JI Am. J. Physiol.-Gastroint. Liver Physiol. PD SEP PY 1997 VL 273 IS 3 BP G754 EP G758 PG 5 WC Gastroenterology & Hepatology; Physiology SC Gastroenterology & Hepatology; Physiology GA XV767 UT WOS:A1997XV76700022 PM 9316481 ER PT J AU Prabhu, SD Rozek, MM Murray, DR Freeman, GL AF Prabhu, SD Rozek, MM Murray, DR Freeman, GL TI Ryanodine and left ventricular function in intact dogs: dissociation of force-based and velocity-based indexes SO AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY LA English DT Article DE cardiac mechanics; calcium handling; ventricular contraction; ventricular relaxation ID CLOSED-CHEST DOGS; SARCOPLASMIC-RETICULUM; INTRACELLULAR CA-2+; CARDIAC-MUSCLE; CONTRACTILE STATE; VOLUME RELATION; CONSCIOUS DOGS; PRESSURE; HEART; HYPERTROPHY AB After anesthesia and autonomic blockade, nine dogs chronically instrumented with left ventricular (LV) micromanometers and piezoelectric dimension crystals were studied before and after the intravenous administration of 4 mu g/kg ryanodine, a specific inhibitor of the sarcoplasmic reticulum Ca2+ release channel. Ryanodine prolonged LV contraction and relaxation (P < 0.001) without changing heart rate, end-diastolic volume (EDV), or end-systolic pressure. Velocity-dependent mechanical parameters were significantly depressed, including the maximal rate of LV pressure rise (dP/dt(max); P < 0.002), the mean velocity of circumferential fiber shortening (P < 0.002), the slope of the dP/dt(max)-EDV relation (P < 0.05), and the time constant of LV relaxation (P < 0.01). In contrast, the slopes of the end-systolic pressure-volume (P-ES-V-ES) and stroke work (SW)-EDV relations, both force-based parameters, were increased (P < 0.05) or maintained, respectively. Ryanodine reduced overall LV contractile performance, evidenced by significant rightward shifts of the P-ES-V-ES, dP/dt(max)-EDV, and SW-EDV relations and reduced SW at constant preload (P < 0.02). Thus, in the dosed-chest dog, low-dose ryanodine resulted in 1) generalized slowing of LV mechanical events without changes in heart rate or load, 2) dissociation of velocity-based and force-based measures of LV function, with depression of the former but enhancement or maintenance of the latter, and 3) reduced overall LV inotropic performance. These effects are consistent with ryanodine-induced alterations of the Ca2+ transient and altered sarcoplasmic reticulum Ca2+ availability. C1 Univ Texas, Hlth Sci Ctr, Dept Med, San Antonio, TX 78284 USA. Audie L Murphy Mem Vet Hosp, San Antonio, TX 78284 USA. RP Prabhu, SD (reprint author), Univ Texas, Hlth Sci Ctr, Dept Med Cardiol, 7703 Floyd Curl Dr, San Antonio, TX 78284 USA. RI Prabhu, Sumanth/D-5223-2009 NR 36 TC 8 Z9 8 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0363-6135 J9 AM J PHYSIOL-HEART C JI Am. J. Physiol.-Heart Circul. Physiol. PD SEP PY 1997 VL 273 IS 3 BP H1561 EP H1568 PG 8 WC Cardiac & Cardiovascular Systems; Physiology; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Physiology GA XV709 UT WOS:A1997XV70900063 ER PT J AU Roesler, J Srivatsan, E Moatamed, F Peters, J Livingston, EH AF Roesler, J Srivatsan, E Moatamed, F Peters, J Livingston, EH TI Tumor suppressor activity of neural cell adhesion molecule in colon carcinoma SO AMERICAN JOURNAL OF SURGERY LA English DT Article ID LUNG-CANCER; N-CAM; GENE-PRODUCT; COLORECTAL-CANCER; DCC GENE; EXPRESSION; GROWTH; NCAM; IDENTIFICATION; ASSOCIATION AB BACKGROUND AND AIMS: Neural Cell Adhesion Molecule (NCAM) is a well-characterized member of the immunoglobin superfamily. The structure of NCAM is similar to the tumor suppressor Deleted in Colon Carcinoma (DCC). NCAM has been found in some epithelial tissues and plays a role in tumorigenesis of some cancers. The purpose of the present study was to determine if NCAM is present in normal human colon. Once its presence was established, its function as a tumor suppressor was investigated. METHODS: Colon tumors and normal proximal margins were processed for reverse transcription-polymerase chain reaction (RT-PCR) of the NCAM-180 message. Immunohistochemistry of the tissue was performed to determine the distribution of NCAM. RESULTS: RT-PCR analysis demonstrated the presence of the NCAM-180 kD isoform in normal colonic epithelia. Immunohistochemistry showed NCAM on the basolateral surface of colonic epithelial cells of the villous tips. Tumors from 15 patients followed up for 4 years were studied. All seven tumors expressing NCAM-180 were from patients having a benign clinical course. Seven of eight tumors that lacked NCAM-180 were associated with aggressive clinical behaviors (presenting with obstruction, perforation or metastatic disease, or patient death within 18 months of presentation). The sole exception was in a villous adenoma excised from a patient who has had multiply recurrent polyps on follow-up. CONCLUSION: We conclude that like DCC, NCAM is an important colonic adhesion molecule that functions as a tumor suppressor. (C) 1997 by Excerpta Medica, Inc. C1 UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,SURG PATHOL SERV,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,RES SERV,LOS ANGELES,CA 90073. CHILDRENS HOSP LOS ANGELES,DEPT PATHOL,LOS ANGELES,CA 90027. UNIV SO CALIF,LOS ANGELES,CA 90089. NR 26 TC 49 Z9 49 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9610 J9 AM J SURG JI Am. J. Surg. PD SEP PY 1997 VL 174 IS 3 BP 251 EP 257 DI 10.1016/S0002-9610(97)00142-6 PG 7 WC Surgery SC Surgery GA XX314 UT WOS:A1997XX31400007 PM 9324132 ER PT J AU Vetto, JT Lum, S Morris, A Sicotte, M Davis, J Lemon, M Weinberg, A AF Vetto, JT Lum, S Morris, A Sicotte, M Davis, J Lemon, M Weinberg, A TI Presence of the T-cell activation marker OX-40 on tumor infiltrating lymphocytes and draining lymph node cells from patients with melanoma and head and neck cancers SO AMERICAN JOURNAL OF SURGERY LA English DT Article; Proceedings Paper CT 87th Annual Meeting of the American-Association-for-Cancer-Research CY APR 20-24, 1996 CL WASHINGTON, DC SP Amer Assoc Canc Res ID EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; NECROSIS-FACTOR; SPINAL-CORD; OX40 LIGAND; ANTIGEN; SYSTEM; RAT; SUBPOPULATIONS; IDENTIFICATION; EXPRESSION AB BACKGROUND: The OX-40 antigen is a cell surface glycoprotein in the tumor necrosis factor receptor family that is expressed primarily on activated CD4+ T cells, Selective target organ expression of the OX-40 receptor on autoantigen specific T cells has been found in autoimmune disease, In order to evaluate whether OX-40 is expressed on T cells from patients with nodal-draining carcinomas, OX-40 expression was assessed in tumor infiltrating lymphocytes (TILs), draining lymph node cells (DLNCs), and/or peripheral blood lymphocytes (PBLs) of 13 patients with head and neck squamous cell carcinomas and 9 patients with melanomas. METHODS: Cell phenotype was determined by fluorescence cell analysis using a monoclonal anti-body to human OX-40, and CD4+ T cell lymphokine production was determined by reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: Expression of the OX-40 receptor was found in as many as 31% of the TILs and as many as 28% of the DLNCs tested, Conversely, no OX-40 expression was found in PBLs, In addition, CD4+ T cells isolated from DLNCs (but not from TILs or PBLs) secreted a Th1 pattern of cytokines (IL-2, gamma interferon), Go-culture of autologous CD4+ TILs with an MHC class II+ melanoma cell line transfected with OX-40 ligand cDNA resulted in T cell proliferation and in vitro tumor regression, CONCLUSIONS: These findings suggest that OX-40+ CD4+ T cells isolated from tumors and their adjacent draining nodes may represent a tumor-specific population of activated T cells capable of mediating tumor reactivity, These cells may play an exploitable role in future trials of immunotherapy, (C) 1997 by Excerpta Medica, Inc. C1 EARLE A CHILES RES INST,PROVIDENCE PORTLAND MED CTR,PORTLAND,OR. RP Vetto, JT (reprint author), OREGON HLTH SCI UNIV,DEPT SURG,SECT SURG ONCOL,PORTLAND VET AFFAIRS MED CTR,L223A,PORTLAND,OR 97201, USA. NR 35 TC 69 Z9 71 U1 2 U2 3 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9610 J9 AM J SURG JI Am. J. Surg. PD SEP PY 1997 VL 174 IS 3 BP 258 EP 265 DI 10.1016/S0002-9610(97)00139-6 PG 8 WC Surgery SC Surgery GA XX314 UT WOS:A1997XX31400008 PM 9324133 ER PT J AU Ebrahimi, SA Sawicki, MP AF Ebrahimi, SA Sawicki, MP TI Tracking the MEN1 gene SO AMERICAN JOURNAL OF SURGERY LA English DT Article ID SUSCEPTIBILITY GENE; OVARIAN-CANCER; IDENTIFICATION; CANDIDATE; BREAST; RETINOBLASTOMA; CHROMOSOME-11; 11Q13 AB Multiple endocrine neoplasia type 1 (MEN1) is a hereditary autosomal dominant disease characterized by parathyroid hyperplasia, pancreatic endocrine tumors, and pituitary adenomas. Sporadic forms of these tumors are more common than their inherited counterparts and share common genetic abnormalities. We have been studying the molecular genetics of sporadic pancreatic endocrine tumors to identify the tumor suppressor gene responsible for MEN1 by positional cloning, This review introduces the reader to the fundamentals of these molecular genetic techniques and outlines the general strategy used to isolate this gene. (C) 1997 by Excerpta Medica, Inc. C1 W LOS ANGELES VET AFFAIRS MED CTR,DEPT SURG W112,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA 90024. NR 23 TC 4 Z9 4 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9610 J9 AM J SURG JI Am. J. Surg. PD SEP PY 1997 VL 174 IS 3 BP 266 EP 270 DI 10.1016/S0002-9610(97)00143-8 PG 5 WC Surgery SC Surgery GA XX314 UT WOS:A1997XX31400009 PM 9324134 ER PT J AU Schwid, HA Rooke, A Ross, BK Sivarajan, M AF Schwid, HA Rooke, A Ross, BK Sivarajan, M TI Use of a computerized ACLS simulator improves performance of ACLS guidelines SO ANESTHESIOLOGY LA English DT Meeting Abstract C1 UNIV WASHINGTON,DEPT ANESTHESIOL,SEATTLE,WA 98108. VA PUGET SOUND HLTH CARE SYST,ANESTHESIA SERV,SEATTLE,WA 98108. NR 0 TC 5 Z9 5 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0003-3022 J9 ANESTHESIOLOGY JI Anesthesiology PD SEP PY 1997 VL 87 IS 3 SU S BP A934 EP A934 DI 10.1097/00000542-199709001-00934 PG 1 WC Anesthesiology SC Anesthesiology GA XV636 UT WOS:A1997XV63600934 ER PT J AU Rudick, R Antel, J Confavreux, C Cutter, G Ellison, G Fischer, J Lublin, F Miller, A Petkau, J Rao, S Reingold, S Syndulko, K Thompson, A Wallenberg, J Weinshenker, B Willoughby, E AF Rudick, R Antel, J Confavreux, C Cutter, G Ellison, G Fischer, J Lublin, F Miller, A Petkau, J Rao, S Reingold, S Syndulko, K Thompson, A Wallenberg, J Weinshenker, B Willoughby, E TI Recommendations from the national multiple sclerosis society clinical outcomes assessment task force SO ANNALS OF NEUROLOGY LA English DT Article ID PROGRESSION AB This article provides recommendations from the National Multiple Sclerosis Society's Clinical Outcomes Assessment Task Force. The Task Force was appointed in 1994 and charged with recommendending improved approaches for clinical outcomes assessment in future controlled clinical trials. The recommendations herein follow extensive deliberation and data analysis during 2.5 years. General principles and desirable measurement attributes were used to assess alternative measurement techniques and clinical scales. On the basis of the analysis of existing multiple sclerosis (MS) data sets, a new measurement approach is proposed. The approach is based on quantitative functional composites that consist of simple quantitative measures from the major clinical dimensions of MS combined into a single score. Quantitative functional composites are likely to provide improved precision and sensitivity in future MS clinical trials. Studies necessary to further refine quantitative functional composites as useful MS clinical trial outcomes are delineated. C1 MCGILL UNIV,MONTREAL NEUROL INST,MONTREAL,PQ,CANADA. HOP DE ANTIQUAILLE,LYON,FRANCE. AMC,CTR CANC RES,DENVER,CO. UNIV CALIF LOS ANGELES,MED CTR,LOS ANGELES,CA 90024. MAIMONIDES HOSP,BROOKLYN,NY 11219. UNIV BRITISH COLUMBIA,VANCOUVER,BC V5Z 1M9,CANADA. MED COLL WISCONSIN,MILWAUKEE,WI 53226. NATL MULTIPLE SCLEROSIS SOC,NEW YORK,NY. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. INST NEUROL,LONDON WC1N 3BG,ENGLAND. BERLEX LABS INC,RICHMOND,CA. MAYO CLIN,ROCHESTER,MN. AUCKLAND HOSP,AUCKLAND,NEW ZEALAND. RP Rudick, R (reprint author), CLEVELAND CLIN FDN,DEPT NEUROL,MELLEN CTR MULTIPLE SCLEROSIS TREATMENT & RES,CLEVELAND,OH 44106, USA. RI Rao, Stephen/A-2460-2010; Thompson, Alan/C-2654-2008 OI Rao, Stephen/0000-0002-6463-7460; Thompson, Alan/0000-0002-4333-8496 NR 15 TC 230 Z9 233 U1 0 U2 2 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0364-5134 J9 ANN NEUROL JI Ann. Neurol. PD SEP PY 1997 VL 42 IS 3 BP 379 EP 382 DI 10.1002/ana.410420318 PG 4 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA XV476 UT WOS:A1997XV47600017 PM 9307263 ER PT J AU Graybill, JR Bocanegra, R Luther, M Fothergill, A Rinaldi, MJ AF Graybill, JR Bocanegra, R Luther, M Fothergill, A Rinaldi, MJ TI Treatment of murine Candida krusei or Candida glabrata infection with L-743,872 SO ANTIMICROBIAL AGENTS AND CHEMOTHERAPY LA English DT Article ID FLUCONAZOLE RESISTANCE; RISK-FACTORS; IN-VITRO; PNEUMOCANDIN; NEUTROPENIA; L-733560; L-705589; L-731373 AB L-743,872 is a broad-spectrum pneumocandin antifungal drug developed by Merck Research Co., and in the present work it was evaluated in vivo in murine models of Candida krusei and Candida glabrata infection, Mice were infected intravenously with two isolates of C, krusei and treated with fluconazole or L-743,872, Fluconazole was beneficial only in immune-competent mice infected with isolate 94-2696. At >0.5 mg/kg of body weight/day, L-743,872 was effective against both infecting isolates in immune-competent and immune-suppressed mice. Against C,glabrata, L-743,872 nas effective, at doses greater than or equal to 0.5 mg/kg, in reducing fungal cell counts in the kidneys but not in the spleen, L-743,872 has significant potential for clinical development. C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX 78284. RP Graybill, JR (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. NR 14 TC 59 Z9 59 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0066-4804 J9 ANTIMICROB AGENTS CH JI Antimicrob. Agents Chemother. PD SEP PY 1997 VL 41 IS 9 BP 1937 EP 1939 PG 3 WC Microbiology; Pharmacology & Pharmacy SC Microbiology; Pharmacology & Pharmacy GA XV701 UT WOS:A1997XV70100017 PM 9303388 ER PT J AU Bonomo, RA Knox, JR Rudin, SD Shlaes, DM AF Bonomo, RA Knox, JR Rudin, SD Shlaes, DM TI Construction and characterization of an OHIO-1 beta-lactamase bearing Met69Ile and Gly238Ser mutations SO ANTIMICROBIAL AGENTS AND CHEMOTHERAPY LA English DT Article ID ESCHERICHIA-COLI; CONFERRING RESISTANCE; EXTENDED-SPECTRUM; CLAVULANIC ACID; SHV-1 FAMILY; MUTAGENESIS; INHIBITORS; MUTANTS; CEPHALOSPORINS; ARGININE-244 AB Amino acid changes that influence activity and resistance to beta-lactams and beta-lactamase inhibitors were explored by constructing the Gly238Ser and Met69Ile-Gly238Ser mutants of the OHIO-1 beta-lactamase, a class A enzyme of the SHV family, The K-m values of cefotaxime and ceftazidime for OHIO-1 and Met69Ile beta-lactamases were greater than or equal to 500 mu M. The K-m of cefotaxime for the Gly238Ser beta-lactamase was 26 mu M, and that of ceftazidime was 105 mu M. The K-m of cefotaxime for the Met69Ile-Gly238Ser beta-lactamase was 292 mu M, and that of ceftazidime was 392 mu M, For the beta-lactamase inhibitors clavulanate and sulbactam, the apparent K-i values for the Met69Ile-Gly238Ser enzyme were 0.03 and 0.15 mu M, respectively, Relative V-max values indicate that the Met69Ile-Gly238Ser mutant of the OHIO-1 beta-lactamase possesses cephalosporinase activity similar to that of the Gly238Ser mutant but diminished penicillinase activity,In an Escherichia coli DH5 alpha strain that possesses a Met69Ile beta-lactamase of the OHIO-1 family, the added Gly238Ser mutation resulted in a phenotype with qualities that confer resistance to expanded-spectrum cephalosporins and, to a lesser extent, beta-lactamase inhibitors. C1 US DEPT VET AFFAIRS,MED CTR,RES SERV,CLEVELAND,OH 44106. CASE WESTERN RESERVE UNIV,DEPT MED,CLEVELAND,OH 44106. UNIV CONNECTICUT,DEPT MOL & CELL BIOL,STORRS,CT 06269. WYETH AYERST RES,INFECT DIS RES,PEARL RIVER,NY 10965. FU NIA NIH HHS [1 KO8 AG 00684-01] NR 28 TC 11 Z9 11 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0066-4804 J9 ANTIMICROB AGENTS CH JI Antimicrob. Agents Chemother. PD SEP PY 1997 VL 41 IS 9 BP 1940 EP 1943 PG 4 WC Microbiology; Pharmacology & Pharmacy SC Microbiology; Pharmacology & Pharmacy GA XV701 UT WOS:A1997XV70100018 PM 9303389 ER PT J AU Roger, L Masi, AT Luther, M AF Roger, L Masi, AT Luther, M TI High clinical remission rates following early therapy of active rheumatoid arthritis (RA) with aurothioglucose and low-dose triamcinolone. SO ARTHRITIS AND RHEUMATISM LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. UNIV ILLINOIS,COLL MED,PEORIA,IL 61656. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0004-3591 J9 ARTHRITIS RHEUM JI Arthritis Rheum. PD SEP PY 1997 VL 40 IS 9 SU S BP 126 EP 126 PG 1 WC Rheumatology SC Rheumatology GA XY634 UT WOS:A1997XY63400126 ER PT J AU Roger, L Masi, AT Luther, M AF Roger, L Masi, AT Luther, M TI Clinical remission in rheumatoid arthritis correlates with entry status: A longitudinal study of 191 patients treated with aurothioglucose and low-dose triamcinolone. SO ARTHRITIS AND RHEUMATISM LA English DT Meeting Abstract C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. UNIV ILLINOIS,COLL MED,PEORIA,IL 61656. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0004-3591 J9 ARTHRITIS RHEUM JI Arthritis Rheum. PD SEP PY 1997 VL 40 IS 9 SU S BP 127 EP 127 PG 1 WC Rheumatology SC Rheumatology GA XY634 UT WOS:A1997XY63400127 ER PT J AU Minghetti, PP Drlica, K Zhang, HG Blackburn, WD AF Minghetti, PP Drlica, K Zhang, HG Blackburn, WD TI TNF-alpha ribozymes and antisense molecules as potential therapeutic agents for treatment of rheumatoid arthritis. SO ARTHRITIS AND RHEUMATISM LA English DT Meeting Abstract C1 UNIV ALABAMA,BIRMINGHAM VA MED CTR,BIRMINGHAM,AL. PUBL HLTH RES INST,NEW YORK,NY. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0004-3591 J9 ARTHRITIS RHEUM JI Arthritis Rheum. PD SEP PY 1997 VL 40 IS 9 SU S BP 306 EP 306 PG 1 WC Rheumatology SC Rheumatology GA XY634 UT WOS:A1997XY63400305 ER PT J AU Zhou, T Zhang, HG Edwards, CK Bluethmann, H Mountz, JD AF Zhou, T Zhang, HG Edwards, CK Bluethmann, H Mountz, JD TI Induction of specific T cell tolerance by Fas ligand expressing antigen presenting cells. SO ARTHRITIS AND RHEUMATISM LA English DT Meeting Abstract C1 AMGEN INC,BOULDER,CO 80301. UNIV ALABAMA,BIRMINGHAM VAMC,BIRMINGHAM,AL 35294. F HOFFMANN LA ROCHE & CO LTD,CH-4002 BASEL,SWITZERLAND. NR 0 TC 0 Z9 0 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0004-3591 J9 ARTHRITIS RHEUM JI Arthritis Rheum. PD SEP PY 1997 VL 40 IS 9 SU S BP 517 EP 517 PG 1 WC Rheumatology SC Rheumatology GA XY634 UT WOS:A1997XY63400516 ER PT J AU Wintersberger, W Fleck, M Edwards, CK Mountz, JD AF Wintersberger, W Fleck, M Edwards, CK Mountz, JD TI RA synovial T cells exhibit susceptibility to Fas apoptosis which is inhibited by TNF. SO ARTHRITIS AND RHEUMATISM LA English DT Meeting Abstract C1 AMGEN INC,BOULDER,CO 80301. BIRMINGHAM VAMC,BIRMINGHAM,AL 35294. UNIV ALABAMA,BIRMINGHAM,AL 35294. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0004-3591 J9 ARTHRITIS RHEUM JI Arthritis Rheum. PD SEP PY 1997 VL 40 IS 9 SU S BP 535 EP 535 PG 1 WC Rheumatology SC Rheumatology GA XY634 UT WOS:A1997XY63400534 ER PT J AU Su, X Hsu, HC Zhou, T Mountz, JD AF Su, X Hsu, HC Zhou, T Mountz, JD TI Increased DNA damage associated with decreased apoptosis in autoimmune SHPTP1-deficient motheaten mice. SO ARTHRITIS AND RHEUMATISM LA English DT Meeting Abstract C1 UNIV ALABAMA,BIRMINGHAM,AL 35294. BIRMINGHAM VAMC,BIRMINGHAM,AL 35294. NR 0 TC 0 Z9 0 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0004-3591 J9 ARTHRITIS RHEUM JI Arthritis Rheum. PD SEP PY 1997 VL 40 IS 9 SU S BP 549 EP 549 PG 1 WC Rheumatology SC Rheumatology GA XY634 UT WOS:A1997XY63400547 ER PT J AU Watanabe, H Sherris, D Gilkeson, GS AF Watanabe, H Sherris, D Gilkeson, GS TI Soluble CD16 in the treatment of murine lupus nephritis. SO ARTHRITIS AND RHEUMATISM LA English DT Meeting Abstract C1 MED UNIV S CAROLINA,CHARLESTON,SC 29425. RALPH H JOHNSON VA MED CTR,CHARLESTON,SC 29425. AREA ADV TECHNOL,RANDOLPH,MA 02368. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0004-3591 J9 ARTHRITIS RHEUM JI Arthritis Rheum. PD SEP PY 1997 VL 40 IS 9 SU S BP 1075 EP 1075 PG 1 WC Rheumatology SC Rheumatology GA XY634 UT WOS:A1997XY63401073 ER PT J AU Oates, J Gilkeson, GS AF Oates, J Gilkeson, GS TI Increased apoptosis of MRL/lpr splenocytes is secondary to elevated nitric oxide production SO ARTHRITIS AND RHEUMATISM LA English DT Meeting Abstract C1 MED UNIV S CAROLINA,CHARLESTON,SC 29414. RALPH H JOHNSON VA MED CTR,CHARLESTON,SC 29414. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0004-3591 J9 ARTHRITIS RHEUM JI Arthritis Rheum. PD SEP PY 1997 VL 40 IS 9 SU S BP 1361 EP 1361 PG 1 WC Rheumatology SC Rheumatology GA XY634 UT WOS:A1997XY63401358 ER PT J AU Silver, DS Silverman, SL AF Silver, DS Silverman, SL TI A comparison of p-DEXA and spine DEXA to hip DEXA in an older postmenopausal population. SO ARTHRITIS AND RHEUMATISM LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES,CEDARS SINAI MED CTR,W LOS ANGELES VA MED CTR,OSTEOPOROSIS MED CTR,LOS ANGELES,CA 90211. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0004-3591 J9 ARTHRITIS RHEUM JI Arthritis Rheum. PD SEP PY 1997 VL 40 IS 9 SU S BP 1477 EP 1477 PG 1 WC Rheumatology SC Rheumatology GA XY634 UT WOS:A1997XY63401474 ER PT J AU Elango, N Vivekananda, J Strong, R Katz, MS AF Elango, N Vivekananda, J Strong, R Katz, MS TI Nuclei isolation from bone cells for nuclear run-on assays SO BIOTECHNIQUES LA English DT Article RP Elango, N (reprint author), UNIV TEXAS,HLTH SCI CTR,S TEXAS VET HLTH CARE SYST,GRECC 182,AUDIE L MURPHY DIV,SAN ANTONIO,TX 78284, USA. NR 4 TC 6 Z9 6 U1 0 U2 0 PU EATON PUBLISHING CO PI NATICK PA 154 E. CENTRAL ST, NATICK, MA 01760 SN 0736-6205 J9 BIOTECHNIQUES JI Biotechniques PD SEP PY 1997 VL 23 IS 3 BP 422 EP 424 PG 3 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA XW102 UT WOS:A1997XW10200015 PM 9298210 ER PT J AU Pedrozo, HA Schwartz, Z Dean, DD Harrison, JL Campbell, JW Wiederhold, ML Boyan, BD AF Pedrozo, HA Schwartz, Z Dean, DD Harrison, JL Campbell, JW Wiederhold, ML Boyan, BD TI Evidence for the involvement of carbonic anhydrase and urease in calcium carbonate formation in the gravity-sensing organ of Aplysia californica SO CALCIFIED TISSUE INTERNATIONAL LA English DT Article DE calcium carbonate; Aplysia californica; statoconia; urease; carbonic anhydrase ID PARATHYROID-HORMONE; LOCALIZATION; OSTEOCLASTS; CALCITONIN; CRYSTALS AB To better understand the mechanisms that could modulate the formation of otoconia, calcium carbonate granules in the inner ear of vertebrate species, we examined statoconia formation in the gravity-sensing organ, the statocyst, of the gastropod mollusk Aplysia californica using an in vitro organ culture model. We determined the type of calcium carbonate present in the statoconia and investigated the role of carbonic anhydrase (CA) and urease in regulating statocyst pH as well as the role of protein synthesis and urease in statoconia production and homeostasis in vitro. The type of mineral present in statoconia was found to be aragonitic calcium carbonate. When the CA inhibitor, acetazolamide (AZ), was added to cultures of statocysts, the pH initially (30 min) increased and then decreased. The urease inhibitor, acetohydroxamic acid (AHA), decreased statocyst pH. Simultaneous addition of AZ and AHA caused a decrease in pH. Inhibition of urease activity also reduced total statoconia number, but had no effect on statoconia volume. Inhibition of protein synthesis reduced statoconia production and increased statoconia volume. In a previous study, inhibition of CA was shown to decrease statoconia production. Taken together, these data show that urease and CA play a role in regulating statocyst pH and the formation and maintenance of statoconia. CA produces carbonate ion for calcium carbonate formation and urease neutralizes the acid formed due to CA action, by production of ammonia. C1 UNIV TEXAS,HLTH SCI CTR,DEPT ORTHOPAED,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT PHYSIOL,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT OTOLARYNGOL HEAD & NECK SURG,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT PERIODONT,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT BIOCHEM,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. HEBREW UNIV JERUSALEM,HADASSAH FAC DENT MED,DEPT PERIODONT,JERUSALEM,ISRAEL. RICE UNIV,DEPT BIOCHEM,HOUSTON,TX 77251. OI Dean, David/0000-0002-4512-9065 NR 49 TC 12 Z9 13 U1 0 U2 5 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0171-967X J9 CALCIFIED TISSUE INT JI Calcif. Tissue Int. PD SEP PY 1997 VL 61 IS 3 BP 247 EP 255 DI 10.1007/s002239900330 PG 9 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XR504 UT WOS:A1997XR50400013 PM 9262517 ER PT J AU Lewis, BS Kattan, D Laughrun, D AF Lewis, BS Kattan, D Laughrun, D TI Use and limitations of immediate postprocedural intracoronary Doppler blood flow measurements for predicting late result after coronary balloon angioplasty - Results of a pilot study SO CARDIOLOGY LA English DT Article DE coronary flow reserve; intracoronary Doppler; coronary blood flow; balloon angioplasty; coronary restenosis ID LUMINAL DIAMETER; LATE RESTENOSIS; ELASTIC RECOIL; ARTERY DISEASE; GUIDE-WIRE; VELOCITY; RESERVE; ATHERECTOMY; HYPERPLASIA; MORPHOLOGY AB Background and Aims: Physiologic measurement of myocardial perfusion in the immediate postangioplasty period may complement the angiographic assessment of the outcome of the procedure and improve our ability to identify patients at increased risk for a suboptimal late result. Immediate in-lab identification of patients at risk for late coronary restenosis would allow the interventionalist to implement alternate interventional and/or pharmacologic strategies aimed at improving the long-term outcome of angioplasty. The present single-center pilot study was undertaken to examine prospectively the value of intracoronary Doppler flow measurements immediately postangioplasty for predicting long-term patency of the dilated coronary artery. Patients and Methods: Coronary average peak flow velocity (APV) at rest and during hyperemia (6-18 mu g intracoronary adenosine) and coronary flow reserve in the distal coronary segment were measured in 24 consecutive patients 10-15 min after successful elective coronary angioplasty. Volume flow (Q) was calculated as APV/2.coronary cross-sectional area heart rate. Coronary arterial vessels and narrowings were measured by quantitative angiography using a geometric based method and automated edge detection. The present study reports the findings in the 16 patients undergoing conventional balloon angioplasty for whom hard endpoint angiographic data were available 4.9 +/- 1.5 months after angioplasty. Results: A linear relation was present between angiographically measured minimal luminal dimension immediately postangioplasty and the late angiographic result of the procedure (r = 0.71, p = 0.0005). A greater acute gain during angioplasty was predictive of a larger luminal dimension at late angiographic follow-up (p = 0.006). There was no relation between the immediate postangioplasty Doppler flow measurements and the late angiographic result of the procedure. Late luminal dimension was not related to immediate postangioplasty basal or hyperemia APV, nor to immediate postangioplasty basal or hyperemic volume flow or to coronary flow reserve (all NS). Conclusions: In this single-center study, intracoronary blood flow and Doppler-derived coronary flow reserve immediately postpercutaneous transluminal coronary angioplasty were not predictive of long-term vessel patency or late coronary restenosis. The immediate angiographic result of angioplasty did correlate with the late result of the procedure. C1 UNIV CALIF LOS ANGELES,SCH MED,CARDIOL SECT,W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90024. TECHNION ITT,BRUCE RAPPAPORT SCH MED,HAIFA,ISRAEL. NR 39 TC 0 Z9 0 U1 0 U2 1 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0008-6312 J9 CARDIOLOGY JI Cardiology PD SEP-OCT PY 1997 VL 88 IS 5 BP 433 EP 440 DI 10.1159/000177373 PG 8 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA XR992 UT WOS:A1997XR99200007 PM 9286505 ER PT J AU Tu, SP McDonell, MB Spertus, JA Steele, BG Fihn, SD AF Tu, SP McDonell, MB Spertus, JA Steele, BG Fihn, SD TI A new self-administered questionnaire to monitor health-related quality of life in patients with COPD SO CHEST LA English DT Article DE chronic obstructive pulmonary disease; functional status; health-related quality of life; questionnaire ID OBSTRUCTIVE PULMONARY-DISEASE; CHRONIC RESPIRATORY QUESTIONNAIRE; OF-LIFE; EFFICACY; CRQ AB Study objective: To develop and validate a brief, computer-scannable, self-administered questionnaire to monitor health-related quality of life in patients with COPD. The Seat-tie Obstructive Lung Disease Questionnaire (SOLQ) consists of 29 items measuring four health dimensions: physical function, emotional function, coping skills, and treatment satisfaction. Methods: A series of studies was performed to assess reliability, validity, and responsiveness, Internal consistency was measured using a cross-sectional survey of 203 COPD patients, Reproducibility was tested over a 4-month interval among 97 patients with self-reported stable conditions, To assess construct validity, SOLO scales were correlated with corresponding Chronic Respiratory Disease Questionnaire (CRDQ) scales, the CORD Self-Efficacy Scale (CSES), percent predicted FEV1, and 6-min walk test, Treatment satisfaction scores of 920 subjects were correlated with a general measure of patient satisfaction. Baseline and follow-up scores of subjects were compared to assess treatment responsiveness. Results: SOLQ scales were reliable (Cronbach's alpha 0.79 to 0.93, and intraclass correlation coefficients 0.64 to 0.87). Change in SOLO scores correlated with corresponding CRDQ scales: dyspnea, r = 0.42; emotional burden, r = 0.49; mastery, r = 0.36. Coping skills correlated highly with CSES, r = 0.93, Treatment satisfaction correlation was r = 0.54. Significant changes occurred in all three scales postintervention. Conclusion: The SOLO is a reliable, valid, and responsive measure of physical and emotional function, coping skills, and treatment satisfaction, Brief, self-administered, and computer scannable, it is useful in monitoring long-term outcomes among large groups of COPD patients. C1 UNIV WASHINGTON,DEPT INTERNAL MED,SEATTLE,WA 98195. VET AFFAIRS PUGET SOUND HLTH CARE SYST,HLTH SERV RES & DEV PROGRAM,SEATTLE,WA. VET AFFAIRS PUGET SOUND HLTH CARE SYST,DEPT PULM MED,SEATTLE,WA. UNIV MISSOURI,DEPT CARDIOL,KANSAS CITY,MO 64110. NR 25 TC 56 Z9 65 U1 0 U2 2 PU AMER COLL CHEST PHYSICIANS PI NORTHBROOK PA 3300 DUNDEE ROAD, NORTHBROOK, IL 60062-2348 SN 0012-3692 J9 CHEST JI Chest PD SEP PY 1997 VL 112 IS 3 BP 614 EP 622 DI 10.1378/chest.112.3.614 PG 9 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA XX778 UT WOS:A1997XX77800009 PM 9315792 ER PT J AU Siegel, R AF Siegel, R TI Community-acquired pneumonia - Need for a cost-effective approach to treatment - Response SO CHEST LA English DT Letter ID THERAPY RP Siegel, R (reprint author), MT SINAI SCH MED,BRONX VET AFFAIRS MED CTR,NEW YORK,NY 10029, USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU AMER COLL CHEST PHYSICIANS PI NORTHBROOK PA 3300 DUNDEE ROAD, NORTHBROOK, IL 60062-2348 SN 0012-3692 J9 CHEST JI Chest PD SEP PY 1997 VL 112 IS 3 BP 862 EP 862 DI 10.1378/chest.112.3.862 PG 1 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA XX778 UT WOS:A1997XX77800059 ER PT J AU vanLinthoudt, D Beutler, A Clayburne, G Sieck, M Fernandes, L Schumacher, HR AF vanLinthoudt, D Beutler, A Clayburne, G Sieck, M Fernandes, L Schumacher, HR TI Morphometric studies on synovium in advanced osteoarthritis: Is there an association between apatite-like material and collagen deposits? SO CLINICAL AND EXPERIMENTAL RHEUMATOLOGY LA English DT Article DE apatite-like material; fibrosis; osteoarthritis; synovium ID PYROPHOSPHATE DIHYDRATE CRYSTALS; ALIZARIN RED; OSTEO-ARTHRITIS; FLUID; HYDROXYAPATITE; STIMULATION; MEMBRANE AB Objective. To look for the frequency and the influence of apatite-like deposits in the synovial membrane of advanced osteoarthritic joints. Methods. Synovium of 16 joints undergoing total arthroplasty for advanced primary osteoarthritis was embedded in paraffin. Adjacent sections were stained with alizarin red S, Mowat's pentachrome, Hematoxylin-eosin and Gomori to show apatite-like deposits, collagen, cartilage fragments, vessels, cells and iron respectively. Histomorphometry was carried out for the apatite-like and collagen deposits by the point counting method; the density of vessels and cells was also quantified. Results. 14 out of the 16 specimens contained apatite-like material, mostly on the synovial surface or just beneath. There was no correlation between the apatite-like deposits and any other measured histological parameter (fibrosis, villus length, synovial lining cell width, density of synovial vessels or cells). Interestingly, the p value for a correlation between the amounts of apatite and collagen deposits was close to significance (r = 0.53; p = 0.055) when the relative volume of the apatite-like material was less than 1.2% (n = 13). Conclusions. Apatite-like deposits are frequently observed in the synovium of late stage osteoarthritis. Although not statistically significant these results suggest a possible association between apatite-like and collagen deposits when the amount of apatite deposits is low. Further quantitative studies are recommended to investigate this observation. C1 VET AFFAIRS MED CTR,ARTHRIT IMMUNOL CTR,PHILADELPHIA,PA 19104. HOSP UNIV PENN,DEPT INTERNAL MED,DIV RHEUMATOL,PHILADELPHIA,PA 19104. NR 31 TC 3 Z9 3 U1 0 U2 0 PU CLINICAL & EXPER RHEUMATOLOGY PI PISA PA VIA SANTA MARIA 31, 56126 PISA, ITALY SN 0392-856X J9 CLIN EXP RHEUMATOL JI Clin. Exp. Rheumatol. PD SEP-OCT PY 1997 VL 15 IS 5 BP 493 EP 497 PG 5 WC Rheumatology SC Rheumatology GA XW695 UT WOS:A1997XW69500005 PM 9307856 ER PT J AU Shlaes, DM Gerding, DN John, JF Craig, WA Bornstein, DL Duncan, RA Eckman, MR Farrer, WE Greene, WH Lorian, V Levy, S McGowan, JE Paul, SM Ruskin, J Tenover, FC Watanakunakorn, C AF Shlaes, DM Gerding, DN John, JF Craig, WA Bornstein, DL Duncan, RA Eckman, MR Farrer, WE Greene, WH Lorian, V Levy, S McGowan, JE Paul, SM Ruskin, J Tenover, FC Watanakunakorn, C TI Society for Healthcare Epidemiology of America and Infectious Diseases Society of America Joint Committee on the Prevention of Antimicrobial Resistance: Guidelines for the prevention of antimicrobial resistance in hospitals (Reprinted from Infection Control and Hospital Epidemiology, vol 18, pg 275-91, 1997) SO CLINICAL INFECTIOUS DISEASES LA English DT Reprint ID GRAM-NEGATIVE BACILLI; STAPHYLOCOCCUS-AUREUS; ANTIBIOTIC USE; AMIKACIN USE; AMINOGLYCOSIDE RESISTANCE; CIPROFLOXACIN RESISTANCE; CEPHALOSPORIN USE; ACTIVE EFFLUX; USAGE; SURVEILLANCE AB Antimicrobial resistance results in increased morbidity, mortality, and costs of health care, Prevention of the emergence of resistance and the dissemination of resistant microorganisms will reduce these adverse effects and their attendant costs. Appropriate antimicrobial stewardship that includes optimal selection, dose, and duration of treatment, as well as control of antibiotic use, will prevent or slow the emergence of resistance among microorganisms. A comprehensively applied infection control program will interdict the dissemination of resistant strains. C1 VET AFFAIRS LAKESIDE MED CTR,CHICAGO,IL. UNIV MED & DENT NEW JERSEY,ROBERT WOOD JOHNSON MED SCH,NEW BRUNSWICK,NJ. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. SUNY HLTH SCI CTR,SYRACUSE,NY 13210. LAHEY CLIN FDN,BURLINGTON,MA. DULUTH CLIN LTD,DULUTH,MN. ST ELIZABETH HOSP,ELIZABETH,NJ. SUNY STONY BROOK,STONY BROOK,NY 11794. BRONX LEBANON HOSP CTR,BRONX,NY 10456. TUFTS UNIV,SCH MED,BOSTON,MA 02111. GRADY MEM HOSP,ATLANTA,GA. NEW JERSEY DEPT HLTH,TRENTON,NJ. KAISER PERMANENTE MED CTR,LOS ANGELES,CA 90034. CTR DIS CONTROL & PREVENT,ATLANTA,GA. ST ELIZABETH HOSP,MED CTR,YOUNGSTOWN,OH 44501. RP Shlaes, DM (reprint author), WYETH AYERST RES,401 N MIDDLETOWN RD,PEARL RIVER,NY 10965, USA. RI mcgowan jr, john/G-5404-2011 NR 85 TC 316 Z9 327 U1 1 U2 13 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD SEP PY 1997 VL 25 IS 3 BP 584 EP 599 DI 10.1086/513766 PG 16 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA XW375 UT WOS:A1997XW37500004 PM 9314444 ER PT J AU Finegold, SM AF Finegold, SM TI Perspective on susceptibility testing of anaerobic bacteria SO CLINICAL INFECTIOUS DISEASES LA English DT Article; Proceedings Paper CT 2nd Meeting of the Anaerobe-Society-of-the-Americas CY JUL 19-21, 1996 CL CHICAGO, IL SP Anaerobe Soc Amer AB Physicians must use empirical treatment initially for anaerobic infections, However, such treatment can be targeted if clinicians establish the nature of the infection and know the usual infecting flora of that type of infection and how the flora may have been modified by the use of antimicrobials. Physicians must also be aware of the usual susceptibility patterns of various anaerobes and nonanaerobes in the hospitals in which they work, Despite cost containment, it is still important to isolate all anaerobes present, to provide at least general identification (and specific identification of key organisms such as the Bacteroides fragilis group), and to keep the organisms alive so that they may be referred elsewhere for definitive identification and susceptibility testing, if indicated. Because resistance is increasing among anaerobes, susceptibility testing is very important. Susceptibility testing should be done when patients are seriously ill, when patients do not respond to therapy or relapse, when there are few data available on a species, when the organisms isolated are frequently resistant, and when patients require prolonged therapy, Periodic surveys of susceptibility patterns should be done on isolates from individual hospitals, Tests most useful for individual patient isolates are the Etest (AB BIODISK, Solna, Sweden), an expensive test, and the microbroth dilution test. Testing should be done on organisms that are the most virulent and most resistant to antimicrobial agents. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90024. RP Finegold, SM (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,INFECT DIS SECT 111 F,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 2 TC 9 Z9 10 U1 0 U2 1 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD SEP PY 1997 VL 25 SU 2 BP S251 EP S253 PG 3 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA XX136 UT WOS:A1997XX13600058 PM 9310696 ER PT J AU Finegold, SM JousimiesSomer, H AF Finegold, SM JousimiesSomer, H TI Recently described clinically important anaerobic bacteria: Medical aspects SO CLINICAL INFECTIOUS DISEASES LA English DT Article; Proceedings Paper CT 2nd Meeting of the Anaerobe-Society-of-the-Americas CY JUL 19-21, 1996 CL CHICAGO, IL SP Anaerobe Soc Amer ID BILOPHILA-WADSWORTHIA BACTEREMIA; SP-NOV; FUSOBACTERIUM-NUCLEATUM; GEN-NOV; HUMAN INFECTIONS; ORAL CAVITY; ACTINOMYCES; PYOGENES; APPENDICITIS; CLOSTRIDIUM AB There is still inadequate information on the role of certain newly described or reclassified anaerobes in disease processes, on their normal sites of carriage, and on their antimicrobial susceptibilities. Herein, we summarize this information (most of the literature reviewed is from the past 5 years, but a few of the articles are similar to 10 years old). Porphyromonas species had seemed to be relatively nonpathogenic, but recent work indicates that this belief is incorrect. P. gingivalis, P. levii-like organisms, and P. endodontalis-like organisms have been recovered from a variety of oral and extraoral infections. P. macacae has been recovered from infected cat bite wounds. Sutterella wadsworthensis, recently differentiated from Campylobacter gracilis, has been found in a variety of infections. Bilophila wadsworthia has also been recovered from a wide variety of infections. Newly described anaerobic cocci, gram-positive nonsporeforming rods, and clostridia have also been isolated from various infections. C1 W LOS ANGELES VET AFFAIRS MED CTR,MICROBIAL DIS RES LAB,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90024. NATL PUBL HLTH INST,ANAEROBE REFERENCE LAB,HELSINKI,FINLAND. RP Finegold, SM (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,INFECT DIS SECT 111 F,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 70 TC 28 Z9 29 U1 1 U2 2 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD SEP PY 1997 VL 25 SU 2 BP S88 EP S93 PG 6 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA XX136 UT WOS:A1997XX13600003 PM 9310641 ER PT J AU Finegold, SM Goldstein, EJC AF Finegold, SM Goldstein, EJC TI Proceedings of the 1996 Meeting of the Anaerobe Society of the Americas - Chicago, Illinois - 19-21 July 1996 - Introduction SO CLINICAL INFECTIOUS DISEASES LA English DT Editorial Material C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90024. SANTA MONICA HOSP MED CTR,MED CTR,RM ALDEN RES LAB,SANTA MONICA,CA. RP Finegold, SM (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,INFECT DIS SECT IIIF,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD SEP PY 1997 VL 25 SU 2 BP S77 EP S77 PG 1 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA XX136 UT WOS:A1997XX13600001 ER PT J AU JousimiesSomer, H AF JousimiesSomer, H TI Recently described clinically important anaerobic bacteria: Taxonomic aspects and update SO CLINICAL INFECTIOUS DISEASES LA English DT Article; Proceedings Paper CT 2nd Meeting of the Anaerobe-Society-of-the-Americas CY JUL 19-21, 1996 CL CHICAGO, IL SP Anaerobe Soc Amer ID 16S RIBOSOMAL-RNA; GRAM-POSITIVE BACTERIA; STREPTOCOCCUS-MILLERI GROUP; NECROPHORUM FLUGGE MOORE; HUMAN GINGIVAL CREVICE; SP-NOV; COMB-NOV; SUBSP-NOV; GEN-NOV; FUSOBACTERIUM-NUCLEATUM AB A new method of identifying bacteria, phylogenetic 16S rRNA sequencing, has led to major reorganizations among most genera of anaerobic bacteria, The pigmented Prevotella species now comprise seven species including P. nigrescens and P. tannerae; P. intermedia/P. nigrescens-like organisms await inclusion, The former Mitsuokella dentalis and Hallella seregens were transferred to Prevotella as one species, P. dentalis. P. enoeca is a new nonpigmenting Prevotella, The genus Porphyromonas currently includes 11 pigmented species and one nonpigmented species, P. catoniae; P. levii-like and P. endodontalis-like organisms are candidates for the genus. Fusobacterium nucleatum currently has five subspecies, and F. varium includes the former F. pseudonecrophorum. Former Wolinella recta and Wolinella curva now are Campylobacter rectus and Campylobacter curvus; Campylobacter showae is a new species. Isolates included in the bile-sensitive former Bacteroides gracilis now are Campylobacter gracilis; the bile-resistant B. gracilis isolates were transferred to a new genus, Sutterella, as S. wadsworthensis. The new Actinomyces species include two subspecies of the A, neuii and the A, radingae-A. turicensis complex. The genus Eubacterium sensu stricto is represented by E. limosum, and the former E. alactolyticum was reclassified in a new genus, Pseudoramibacter, as P. alactolyticus. Recent entries include E. saphenum, E. minutum, E. exiguum, E. infirmum, and E. tardum. A new genus, Atopobium houses some former lactobacilli and streptococci. The genus Peptostreptococcus also have four new species: P. hydrogenalis, P. lacrimalis, P. lactolyticus, and P. vaginalis. C1 W LOS ANGELES VET AFFAIRS MED CTR,MICROBIAL DIS RES LAB,LOS ANGELES,CA 90073. RP JousimiesSomer, H (reprint author), NATL PUBL HLTH INST,ANAEROBE REFERENCE LAB,MANNERHEIMINTIE 166,SF-00300 HELSINKI,FINLAND. NR 89 TC 32 Z9 33 U1 2 U2 5 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD SEP PY 1997 VL 25 SU 2 BP S78 EP S87 PG 10 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA XX136 UT WOS:A1997XX13600002 PM 9310640 ER PT J AU Wexler, HM Molitoris, E Molitoris, D AF Wexler, HM Molitoris, E Molitoris, D TI Susceptibility testing of anaerobes: Old problems, new options? SO CLINICAL INFECTIOUS DISEASES LA English DT Article; Proceedings Paper CT 2nd Meeting of the Anaerobe-Society-of-the-Americas CY JUL 19-21, 1996 CL CHICAGO, IL SP Anaerobe Soc Amer ID BACTERIA C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90024. RP Wexler, HM (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,RES SERV,WADSWORTH DIV,11301 WILSHIRE BLVD 691-151J,LOS ANGELES,CA 90073, USA. NR 11 TC 5 Z9 6 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD SEP PY 1997 VL 25 SU 2 BP S275 EP S278 PG 4 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA XX136 UT WOS:A1997XX13600067 PM 9310705 ER PT J AU Wexler, HM AF Wexler, HM TI Pore-forming molecules in gram-negative anaerobic bacteria SO CLINICAL INFECTIOUS DISEASES LA English DT Article; Proceedings Paper CT 2nd Meeting of the Anaerobe-Society-of-the-Americas CY JUL 19-21, 1996 CL CHICAGO, IL SP Anaerobe Soc Amer ID OUTER-MEMBRANE PROTEINS; FUSOBACTERIUM-NUCLEATUM; BACTEROIDES-FRAGILIS; ESCHERICHIA-COLI; PSEUDOMONAS-AERUGINOSA; CAMPYLOBACTER-JEJUNI; BETA-LACTAMASE; DISULFIDE BOND; OMPA PROTEIN; LAMB PROTEIN AB Little information is available about porin molecules in anaerobes. Porins from Bacteroides fragilis and Porphyromonas, Fusobacterium, and Campylobacter species have been described, A pore-forming outer membrane (OM) porin protein was isolated from B. fragilis (Omp-200); it is exposed at the cell surface and dissociated by boiling and application of reducing agents. Fusobacterium nucleatum FomA, an OM porin protein of 40 kD, had a deduced topology of FomA similar to that of established porins, despite the lack of sequence similarity, An OM preparation from Porphyromonas endodontalis (including a major protein with an apparent molecular mass of 31 kD and other proteins of 40.3-71.6 kD) formed pores in a liposome assay, A major outer membrane protein (MOMP) From Campylobacter jejuni (a microaerophile) is related to the family of trimeric bacterial porins, although little homology was seen with other porins. The development of antimicrobial resistance related to decreased permeability underlines the importance of identifying and characterizing the pore-forming molecules of anaerobes. C1 UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. RP Wexler, HM (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,RES SERV,11301 WILSHIRE BLVD,691-151J,LOS ANGELES,CA 90073, USA. NR 34 TC 9 Z9 9 U1 0 U2 1 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD SEP PY 1997 VL 25 SU 2 BP S284 EP S286 PG 3 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA XX136 UT WOS:A1997XX13600070 PM 9310708 ER PT J AU Anzueto, A Peters, JI Seidner, SR Cox, WJ Schroeder, W Coalson, JJ AF Anzueto, A Peters, JI Seidner, SR Cox, WJ Schroeder, W Coalson, JJ TI Effects of continuous bed rotation and prolonged mechanical ventilation on healthy, adult baboons SO CRITICAL CARE MEDICINE LA English DT Article; Proceedings Paper CT Annual Meeting of the American-College-of-Chest-Physicians CY OCT, 1994 CL NEW ORLEANS, LA SP Amer Coll Chest Physicians DE mechanical ventilation; bronchoalveolar lavage; hemodynamics; lung pathology; continuous bed rotation; Papio cynocephalus; animal model-mechanical ventilation; lung morphology; pulmonary functions; atelectasis ID ACUTE RESPIRATORY-FAILURE; END-EXPIRATORY PRESSURE; KINETIC TREATMENT TABLE; HIGH AIRWAY PRESSURE; INDUCED LUNG INJURY; PULMONARY COMPLICATIONS; PRONE POSITION; NOSOCOMIAL PNEUMONIA; EDEMA; OXYGENATION AB Objective: To study, in a model of prolonged mechanical ventilation, the role of continuous bed rotation on lung function and pathology. Design: Prospective animal study. Setting: Animal research laboratory. Subjects: Healthy adult baboons (Papio cynocephalus), anesthetized with ketamine, sedated, paralyzed, mechanically ventilated for 11 days, and monitored with pulmonary and peripheral arterial catheters. Interventions: Animals were divided into two experimental groups: a)mechanical ventilation alone (control, n = 7); and b) mechanical ventilation with continuous bed rotation therapy to 45 degrees (continuous rotation group, n = 5). Mechanical ventilation was provided far 11 days with an FIO2 of 0.21 and tidal volume of 12 mL/kg. Bronchoalveolar lavage was performed through a fiberoptic bronchoscope. Nursing care procedures, antacids, enteral feeding, and prophylactic antibiotics were administered. Measurements and Main Results: Measurements of hemodynamics, pulmonary functions, lung volumes, arterial blood gases, and chest radiographs were done daily. Bronchoalveolar lavage was performed at days 0, 7, and 11. There were no significant changes in hemodynamics gas exchange, or pulmonary functions during the study period in either group. Microbiological surveillance cultures were negative in both experimental groups. In the control group after 7 days, six of seven animals developed patchy atelectasis; by day 11, two of seven animals demonstrated persistent radiologic abnormalities. Bronchoalveolar ravage neutrophils were significantly increased in control animals at days 7 and 11. Lung pathology in the control group showed areas of bronchiolitis, with surrounding bronchopneumonia in five of seven animals. None of the continuous rotation animals showed any radiologic or morphologic abnormalities. Conclusions: Prolonged mechanical ventilation in the control group resulted in atelectasis, increased concentrations of bronchoalveolar lavage neutrophils, and mild pneumonitis. These effects were not associated with changes in lung volumes, oxygenation, or hemodynamic parameters. Continuous bed rotation helped to prevent these abnormalities. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV PULM DIS CRIT CARE MED,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT PEDIAT,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX 78284. SW FDN BIOMED RES,SAN ANTONIO,TX 78284. KINET CONCEPTS INC,SAN ANTONIO,TX 78284. RP Anzueto, A (reprint author), AUDIE L MURPHY MEM VET HOSP DIV,S TEXAS VET HLTH CARE SYST,PULM DIS SECT 111E,SAN ANTONIO,TX 78284, USA. NR 34 TC 20 Z9 20 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0090-3493 J9 CRIT CARE MED JI Crit. Care Med. PD SEP PY 1997 VL 25 IS 9 BP 1560 EP 1564 DI 10.1097/00003246-199709000-00025 PG 5 WC Critical Care Medicine SC General & Internal Medicine GA XV557 UT WOS:A1997XV55700025 PM 9295832 ER PT J AU Schwartz, MW Prigeon, RL Kahn, SE Nicolson, M Moore, J Morawiecki, A Boyko, EJ Porte, D AF Schwartz, MW Prigeon, RL Kahn, SE Nicolson, M Moore, J Morawiecki, A Boyko, EJ Porte, D TI Evidence that plasma leptin and insulin levels are associated with body adiposity via different mechanisms SO DIABETES CARE LA English DT Article ID OBESE GENE; PROTEIN; EXPRESSION; RNA AB OBJECTIVE - Like insulin, the adipocyte hormone, leptin, circulates at levels proportionate to body adiposity. Because insulin may regulate leptin secretion, we sought to determine if plasma leptin levels are coupled to body adiposity via changes in circulating insulin levels or insulin sensitivity and whether leptin secretion from adipocytes is impaired in subjects with NIDDM. RESEARCH DESIGN AND METHODS - We used multiple linear regression to analyze relationships between BMI (a measure of body adiposity) and fasting plasma levels of leptin and insulin in 98 nondiabetic human subjects (68 men/30 women) and 38 subjects with NIDDM (27 men/11 women). The insulin sensitivity index (S-1) was also determined in a subset of nondiabetic subjects (n = 38). RESULTS - Fasting plasma leptin concentrations were correlated to both BMI (r = 0.66, P = 0.0001) and fasting plasma insulin levels (r = 0.65, P = 0.0001) in nondiabetic men and women (r = 0.58, P = 0.0009 for BMI; r = 0.47, P = 0.01 for insulin). While the plasma leptin level was also inversely related to S-1 (r = -0.35; P = 0.03), this association was dependent on BMI, whereas the association between insulin and S-1 was not. Conversely, the relationship between plasma leptin and BMI was independent of S-1, whereas that between insulin and BMI was dependent on S-1. The relationship between plasma leptin levels and BMI did not differ significantly among NIDDM subjects from that observed in nondiabetic subjects. CONCLUSIONS - We conclude that 1) body adiposity, sex, and the fasting insulin level are independently associated with plasma leptin level; 2) because NIDDM does not influence leptin levels, obesity associated with NIDDM is unlikely to result from impaired leptin secretion; and 3) insulin sensitivity contributes to the association between body adiposity and plasma levels of insulin, but not leptin. The mechanisms underlying the association between body adiposity and circulating levels of these two hormones, therefore, appear to be different. C1 AMGEN INC, THOUSAND OAKS, CA 91320 USA. UNIV WASHINGTON, SCH MED, DEPT MED, SEATTLE, WA 98195 USA. RP Schwartz, MW (reprint author), VET AFFAIRS PUGET SOUND HLTH CARE SYST, 1660 S COLUMBIAN WAY, SEATTLE, WA 98108 USA. RI Schwartz, Michael/H-9950-2012 OI Kahn, Steven/0000-0001-7307-9002; Boyko, Edward/0000-0002-3695-192X FU NIDDK NIH HHS [DK-17844, DK12829]; NINDS NIH HHS [NS-32273] NR 24 TC 81 Z9 81 U1 0 U2 4 PU AMER DIABETES ASSOC PI ALEXANDRIA PA 1701 N BEAUREGARD ST, ALEXANDRIA, VA 22311-1717 USA SN 0149-5992 EI 1935-5548 J9 DIABETES CARE JI Diabetes Care PD SEP PY 1997 VL 20 IS 9 BP 1476 EP 1481 DI 10.2337/diacare.20.9.1476 PG 6 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XT091 UT WOS:A1997XT09100026 PM 9283801 ER PT J AU Ho, M Yip, I Shekelle, PG AF Ho, M Yip, I Shekelle, PG TI Outcomes in diabetic patients - Response SO DIABETES CARE LA English DT Letter C1 W LOS ANGELES VET AFFAIRS MED CTR,GEN MED SECT,MED SERV,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,ENDOCRINOL SECT,MED SERV,LOS ANGELES,CA 90073. VET HLTH ADM SERV,RES & DEV FIELD PROGRAM,CTR STUDY HEALTHCARE PROVIDER BEHAV,LOS ANGELES,CA. NR 2 TC 0 Z9 0 U1 0 U2 0 PU AMER DIABETES ASSOC PI ALEXANDRIA PA 1660 DUKE ST, ALEXANDRIA, VA 22314 SN 0149-5992 J9 DIABETES CARE JI Diabetes Care PD SEP PY 1997 VL 20 IS 9 BP 1497 EP 1498 PG 2 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XT091 UT WOS:A1997XT09100036 ER PT J AU Frazao, JM Levine, BS Tan, AU Mazess, RB Kyllo, DM Knutson, JC Bishop, CW Coburn, JW AF Frazao, JM Levine, BS Tan, AU Mazess, RB Kyllo, DM Knutson, JC Bishop, CW Coburn, JW TI Efficacy and safety of intermittent oral 1 alpha(OH) vitamin D-2 in suppressing 2 degrees hyperparathyroidism in hemodialysis patients SO DIALYSIS & TRANSPLANTATION LA English DT Article; Proceedings Paper CT 29th Annual Meeting of the American-Society-of-Nephrology CY NOV 03-06, 1996 CL NEW ORLEANS, LA SP Amer Soc Nephrol ID DOSE INTRAVENOUS CALCITRIOL; SECONDARY HYPERPARATHYROIDISM; PARATHYROID-HORMONE; DIALYSIS PATIENTS; PROSPECTIVE TRIAL; UREMIC PATIENTS; BONE-DISEASE; CALCIUM; RATS; 1-ALPHA-HYDROXYVITAMIN-D3 AB Calcitriol has a low therapeutic index for the treatment of 2 degrees hyperparathyroidism (HPT) in hemodialysis (HD) patients. In an earlier protocol (Protocol 1), the vitamin D analog, 1 alpha-hydroxyvitamin D-2 1 alpha D-2), was effective in lowering serum intact PTH (iPTH) in HD patients with 2 degrees HPT (iPTH >400 pg/ml) without significant hypercalcemia or hyperphosphatemia; most patients received 4 mu g daily (28 mu g/week), and a few received 4 mu g after each dialysis session (3 times/week). The present protocol (Protocol 2) evaluated the effectiveness and safety of the same starting dose of 10 mu g per HD in all patients (30 mu g/week). Ten patients who had completed Protocol 1 with a dose of 4 mu g daily (6 patients) or 4 mu g three times a week after HD (4 patients) completed Protocol 2. Nine men and 1 woman-ages 27 to 72 years who were dialyzed for between 4 to 116 months-were treated. After a washout period of 8 weeks, 1 alpha D-2 was given for 12 weeks or until iPTH fell below 100 pg/ml. Temporary stop points were a serum phosphorus (P) >8.0 mg/dl or serum calcium (Ca) > 11.4 mg/dl; treatment was resumed at a lower dose when Ca fell to less than or equal to 10.2 mg/dl or P fell to less than or equal to 6.9 mg/dl. Only Ca-based phosphorus binders were used. For the 6 patients treated with 4 mu g/day 1 alpha D-2 in Protocol 1 and later with 10 mu g/3 times a week in Protocol 2, the iPTH decreased from a baseline of 893 +/- 100 pg/ml to a nadir of 215 +/- 67 pg/ml, and from 879 +/- 118 pg/ml to 217 +/- 76 pg/ml, respectively. In Protocol 1, the baseline serum Ca rose from 8.97 +/- 0.31 mg/dl to 9.67 +/- 0.27 mg/dl at the iPTH nadir, and in Protocol 2 from 8.78 +/- 0.18 mg/dl to 9.95 +/- 0.26 mg/dl. For the 4 patients treated in Protocol 1 with 1 alpha D-2 4 mu g/3 times a week and later with 10 mu g/3 times a week, the baseline iPTH fell from 484 +/- 40 pg/ml to a nadir of 160 +/- 22 pg/ml, and from 537 +/- 60 pg/ml to 108 +/- 35 pg/ml, respectively. The serum Ca rose from 8.66 +/- 0.32 mg/dl at baseline to 9.58 +/- 0.45 at iPTH nadir and from 8.66 +/- 0.18 mg/dl to 9.23 +/- 0.39 mg/dl in the first and second protocols, respectively. Serum P levels at baseline and the nadir did not differ with either protocol. Thus, intermittent ''high'' doses of 1 alpha D-2 are as effective as a similar total dose given daily, both treatments ave associated with mild increases in the serum Ca but no change in serum P. Dosing with the dialysis procedure offers the advantage of greater compliance, and the therapeutic index of 1 alpha D-2 appears relatively high with both regimens. RP Frazao, JM (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,WADSWORTH DIV,MED SERV,LOS ANGELES,CA 90073, USA. RI Frazao, Joao/J-9811-2013 OI Frazao, Joao/0000-0002-8081-5474 NR 31 TC 7 Z9 7 U1 0 U2 0 PU CREATIVE AGE PUBL PI VAN NUYS PA 7628 DENSMORE AVE, VAN NUYS, CA 91406-2088 SN 0090-2934 J9 DIALYSIS TRANSPLANT JI Dial. Transplant. PD SEP PY 1997 VL 26 IS 9 BP 583 EP & PG 10 WC Engineering, Biomedical; Transplantation; Urology & Nephrology SC Engineering; Transplantation; Urology & Nephrology GA XW874 UT WOS:A1997XW87400008 ER PT J AU Priefer, BA Robbins, J AF Priefer, BA Robbins, J TI Eating changes in mild-stage Alzheimer's disease: A pilot study SO DYSPHAGIA LA English DT Article DE Alzheimer's disease; eating; swallowing; self-feeding; deglutition; deglutition disorders ID SENILE DEMENTIA; CARE; STROKE; SCALE; HOME AB Eating impairment is well documented in the late stage of Alzheimer's disease (AD) but when these eating changes actually begin in the disease process is not known. Eating was defined as consisting of two components, self-feeding and swallowing. Self-feeding and swallowing of healthy elderly were compared with a group of individuals with mild AD. AD subjects received significantly more partner-initiated cues or direct assistance than controls. In addition, subject-initiated cued behaviors occurred more frequently in the AD group. AD subjects demonstrated significantly prolonged swallow durations for the oral transit duration (cookie), pharyngeal response duration (liquid), and total swallow duration (liquid). This pilot study suggests that self-feeding and swallowing changes may occur early in the course of AD. C1 UNIV WISCONSIN,SCH NURSING,MADISON,WI. UNIV WISCONSIN,DEPT MED,MADISON,WI. UNIV WISCONSIN,DEPT SURG,MADISON,WI. RP Priefer, BA (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,CTR GERIATR RES EDUC & CLIN,2500 OVERLOOK TERRACE,MADISON,WI 53705, USA. NR 54 TC 57 Z9 59 U1 3 U2 9 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0179-051X J9 DYSPHAGIA JI Dysphagia PD FAL PY 1997 VL 12 IS 4 BP 212 EP 221 DI 10.1007/PL00009539 PG 10 WC Otorhinolaryngology SC Otorhinolaryngology GA XR855 UT WOS:A1997XR85500008 PM 9294942 ER PT J AU Martinez, V Cuttitta, F Tache, Y AF Martinez, V Cuttitta, F Tache, Y TI Central action of adrenomedullin to inhibit gastric emptying in rats SO ENDOCRINOLOGY LA English DT Article ID GENE-RELATED PEPTIDE; CORTICOTROPIN-RELEASING FACTOR; PROADRENOMEDULLIN N-TERMINAL-20 PEPTIDE; HYPOTENSIVE PEPTIDE; CONSCIOUS RATS; NERVOUS-SYSTEM; MOTOR FUNCTION; CALCITONIN; RECEPTOR; NUCLEUS AB The central action of human adrenomedullin (AM) to influence gastric emptying and the peripheral mechanisms involved were studied in conscious rats. The 20-min rate of gastric emptying of a methylcellulose solution was assessed after intracisternal (ic) injection of AM or rat alpha-calcitonin gene-related peptide (alpha CGRP). AM and alpha CGRP dose-dependently inhibited gastric emptying with ic ED50 values of 120 and 100 pmol, respectively. Human proadrenomedullin N-terminal 20 peptide (150-600 pmol, ic) and AM (150 pmol, iv) had no effect. The inhibitory actions of AM and alpha CGRP (150 pmol, ic) were completely blocked by the CGRP antagonist, human CGRP-(8-37) injected ic at 30 mu g, but not at 15 mu g. The CRF antagonist, [D-Phe(12),Nle(21,38),C(alpha)MeLeu(37)]CRF-(12-41)(10 mu g/rat) injected in prevented ic rat/human CRF (150 pmol)-induced 53% inhibition of gastric emptying while not modifying the effect of AM. The action of AM (150 pmol, ic) was abolished by bilateral adrenalectomy or the beta-adrenergic blocker, propranolol (1 mg/kg, ip), but was not altered by indomethacin (5 mg/kg, ip) or subdiaphragmatic vagotomy. These results indicate that ic AM and alpha CGRP equipotently inhibit gastric emptying through mechanisms similarly antagonized by a high dose of CGRP(8-37). The central AM action is mediated through adrenal-dependent, beta-adrenergic pathways independently from activation of central CRF receptors. C1 UNIV CALIF LOS ANGELES, SCH MED, INST BRAIN RES, LOS ANGELES, CA 90073 USA. NCI, DIV CANC PREVENT & CONTROL, BIOMARKERS & PREVENT RES BRANCH, NIH, ROCKVILLE, MD 20850 USA. RP Martinez, V (reprint author), UNIV CALIF LOS ANGELES, SCH MED, W LOS ANGELES VET AFFAIRS MED CTR, CURE DIGEST DIS RES CTR, LOS ANGELES, CA 90073 USA. RI Martinez, Vicente/N-1189-2014 NR 51 TC 46 Z9 46 U1 0 U2 0 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0013-7227 EI 1945-7170 J9 ENDOCRINOLOGY JI Endocrinology PD SEP PY 1997 VL 138 IS 9 BP 3749 EP 3755 DI 10.1210/en.138.9.3749 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XR907 UT WOS:A1997XR90700025 PM 9275061 ER PT J AU Brennecke, R Hammermeister, K AF Brennecke, R Hammermeister, K TI Computers and the Internet in cardiac care - Will cardiology rise to this extraordinary opportunity? SO EUROPEAN HEART JOURNAL LA English DT Editorial Material ID OUTCOMES C1 UNIV MAINZ,MED KLIN 3,D-6500 MAINZ,GERMANY. UNIV COLORADO,CTR SCI,BOULDER,CO 80309. DENVER VA MED CTR,DENVER,CO. NR 8 TC 2 Z9 2 U1 0 U2 0 PU W B SAUNDERS CO LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0195-668X J9 EUR HEART J JI Eur. Heart J. PD SEP PY 1997 VL 18 IS 9 BP 1382 EP 1384 PG 3 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA XV988 UT WOS:A1997XV98800008 PM 9458442 ER PT J AU Kizer, KW Fonseca, ML Long, LM AF Kizer, KW Fonseca, ML Long, LM TI The veterans healthcare system: Preparing for the twenty-first century SO HOSPITAL & HEALTH SERVICES ADMINISTRATION LA English DT Article ID FUTURE AB Since its establishment in 1946, the veterans healthcare system has greatly expanded in both size and responsibility. It is now the largest integrated healthcare system in the United States, the nation's largest provider of graduate medical and other health professional training, and one of the largest research enterprises in America. It is also the nation's largest provider of services to homeless persons, an essential provider in the public healthcare safety net, and an increasingly important element in the federal response to disasters and national emergencies. Patterned after what was considered the best in American healthcare, for most of the past 50 years the Department of Veterans Affairs (VA) healthcare has focused primarily on acute inpatient care, high technology, and medical specialization. Now, in response to societal and industrywide forces, the Veterans Health Administration (VHA) is reengineering the veterans healthcare system, changing the operational and management structure from individual hospitals to 22 integrated service networks and transitioning the system to one that is grounded in ambulatory and primary care. This article briefly describes the history and functions of the veterans healthcare system, its service population, and key aspects of its restructuring. C1 VET ADM MED CTR,HLTH SERV RES & DEV SERV,MANAGEMENT DECIS & RES CTR,BOSTON,MA. RP Kizer, KW (reprint author), US DEPT VET AFFAIRS,810 VERMONT AVE NW,ROOM 800,WASHINGTON,DC 20420, USA. NR 20 TC 55 Z9 55 U1 2 U2 6 PU AMER COLL HEALTHCARE EXEC HEALTH ADMINISTRATION PRESS PI CHICAGO PA ONE NORTH FRANKLIN ST SUITE 1700, CHICAGO, IL 60606 SN 8750-3735 J9 HOSP HEALTH SERV ADM JI Hosp. Health Serv. Adm. PD FAL PY 1997 VL 42 IS 3 BP 283 EP 298 PG 16 WC Health Policy & Services SC Health Care Sciences & Services GA XN874 UT WOS:A1997XN87400002 PM 10169289 ER PT J AU Skare, JT Mirzabekov, TA Shang, ES Blanco, DR ErdjumentBromage, H Bunikis, J Bergstrom, S Tempst, P Kagan, BL Miller, JN Lovett, MA AF Skare, JT Mirzabekov, TA Shang, ES Blanco, DR ErdjumentBromage, H Bunikis, J Bergstrom, S Tempst, P Kagan, BL Miller, JN Lovett, MA TI The Oms66 (p66) protein is a Borrelia burgdorferi porin SO INFECTION AND IMMUNITY LA English DT Article ID OUTER-MEMBRANE PROTEIN; LYME-DISEASE SPIROCHETE; FRACTURE ELECTRON-MICROSCOPY; PALLIDUM SUBSP PALLIDUM; TREPONEMA-PALLIDUM; MOLECULAR ANALYSIS; SEQUENCE-ANALYSIS; SURFACE-PROTEINS; SHEATH PROTEIN; DENTICOLA AB In this study we report the purification and characterization of a 66-kDa protein, designated Oms66, for outer membrane-spanning 66-kDa protein, that functions as a porin in the outer membrane (OM) of Borrelia burgdorferi. Oms66 was purified by fast-performance liquid chromatography and exhibited an average single-channel conductance of 9.62 +/- 0.37 nS in 1 M KCI, as evidenced by 581 individual insertional events in planar lipid bilayers, Electrophysiological characterization indicated that Oms66 was virtually nonselective between cations and anions and exhibited voltage-dependent closure with multiple substates, The amino acid sequence of tryptic peptides derived from purified Oms66 was identical to the deduced amino acid sequence of p66, a previously described surface-exposed protein of B. burgdorferi, Purified Oms66 was recognized by antiserum specific for p66 and serum from rabbits immune to challenge with virulent B. burgdorferi, indicating that p66 and Oms66 were identical proteins and that Oms66/p66 is an immunogenic protein in infected rabbits. In a methodology that reduces liposomal trapping and nonspecific interactions, native Oms66 was incorporated into liposomes, confirming that Oms66 is an outer membrane-spanning protein, Proteoliposomes containing Oms66 exhibited porin activity nearly identical to that of native, purified Oms66, indicating that reconstituted Oms66 retained native conformation. The use of proteoliposomes reconstituted with Oms66 and other Oms proteins provides an experimental system for determinating the relationship between conformation, protection, and biological function of these molecules. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,DIV INFECT DIS,LOS ANGELES,CA 90095. UNIV CALIF LOS ANGELES,SCH MED,DEPT PSYCHIAT & BEHAV SCI,LOS ANGELES,CA 90095. UNIV CALIF LOS ANGELES,SCH MED,INST NEUROPSYCHIAT,LOS ANGELES,CA 90095. UNIV CALIF LOS ANGELES,SCH MED,INST BRAIN RES,LOS ANGELES,CA 90095. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. MEM SLOAN KETTERING CANC CTR,PROGRAM MOL BIOL,NEW YORK,NY 10021. UMEA UNIV,DEPT MICROBIOL,S-90187 UMEA,SWEDEN. UNIV CALIF LOS ANGELES,SCH MED,DEPT MICROBIOL & IMMUNOL,LOS ANGELES,CA 90095. RP Skare, JT (reprint author), TEXAS A&M UNIV,HLTH SCI CTR,DEPT MED MICROBIOL & IMMUNOL,COLLEGE STN,TX 77843, USA. FU NIAID NIH HHS [AI-37312, AI-21352, AI-29733] NR 45 TC 67 Z9 67 U1 1 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD SEP PY 1997 VL 65 IS 9 BP 3654 EP 3661 PG 8 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA XT420 UT WOS:A1997XT42000022 PM 9284133 ER PT J AU vonMoll, LK Cantey, JR AF vonMoll, LK Cantey, JR TI Peyer's patch adherence of enteropathogenic Escherichia coli strains in rabbits SO INFECTION AND IMMUNITY LA English DT Article ID DIARRHEIC COMMERCIAL RABBITS; SHIGELLA-FLEXNERI; M-CELLS; WEANLING RABBITS; WEANED RABBITS; RDEC-1; PATHOGENICITY; MECHANISM; INVASION; ATTACHMENT AB RDEC-1 (serotype 015) is an attaching and effacing strain of rabbit enteropathogenic Escherichia coli (REPEC) that causes diarrhea in postweanling rabbits, It expresses AF/R1 pill that mediate Peyer's patch M-cell adherence, We investigated Peyer's patch adherence, the presence of virulence genes, ileal brush border aggregation, and pilus expression in 9 strains representing several serotypes of REPEC as well as in two commensal strains, Postweanling rabbits were inoculated with 10(6) organisms and sacrificed at 24 h, and tissues were prepared for examination by light microscopy, Strains B10 and RDEC-1 were also studied at 12 and 72 h postinoculation, All REPEC strains were eneA positive, expressed pill, and adhered to ileal brush borders, Both commensal strains expressed pill, and one strain adhered to brush borders, All REPEC strains demonstrated some degree of Peyer's patch lymphoid follicle adherence, ranging from diffuse coverage to small patches covering two to three dome epithelial cells, Strains C102 and C110 had genes homologous with the structural subunit gene of the AF/R1 pilus (afrA) of RDEC-1, which correlated with greater degrees of lymphoid follicle adherence and lesser degrees of ileal villus adherence. The observation that all REPEC strains adhere to Peyer's patch epithelium suggests the possibility that human strains of enteropathogenic E. col, (EPEC) might do likewise, EPEC strains might thus serve as mucosal vaccine vectors in humans, Better understanding of the molecular mechanism of REPEC adherence should provide a model for the targeting of the Peyer's patch in humans. C1 RALPH H JOHNSON VA MED CTR, CHARLESTON, SC 29425 USA. RP vonMoll, LK (reprint author), MED UNIV S CAROLINA, DEPT MED, DIV INFECT DIS, 171 ASHLEY AVE, CHARLESTON, SC 29425 USA. NR 29 TC 19 Z9 20 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0019-9567 EI 1098-5522 J9 INFECT IMMUN JI Infect. Immun. PD SEP PY 1997 VL 65 IS 9 BP 3788 EP 3793 PG 6 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA XT420 UT WOS:A1997XT42000042 PM 9284153 ER PT J AU Paulino, AC Fisher, SG Marks, JE AF Paulino, AC Fisher, SG Marks, JE TI Is prophylactic neck irradiation indicated in patients with squamous cell carcinoma of the maxillary sinus? SO INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS LA English DT Article DE maxillary sinus cancer; radiotherapy ID PARA-NASAL SINUSES; RADIATION-THERAPY; POSTOPERATIVE RADIOTHERAPY; ELECTIVE IRRADIATION; MANAGEMENT; ANTRUM; METASTASES; HEAD AB Purpose: To determine the proportion of patients with squamous cell carcinoma of the maxillary sinus who will fail in regional nodes without elective neck treatment and to identify any prognostic factors that may influence neck control. Methods and Materials: From 1971-1995, 42 consecutive patients with squamous cell carcinoma of the maxillary sinus were seen at our department for curative treatment. There were 35 males and 7 females, with a median age at diagnosis of 63.5 years (range, 42-77 years). One tumor was classified as T1, 5 had T2, 15 had T3, and 21 had T4 disease. Four of 42 patients (9.5%) had cervical lymphadenopathy at initial presentation. Thirty-three patients had surgical resection and radiotherapy and nine had radiotherapy alone. None of the 38 patients with clinical N0 necks received elective treatment to the cervical nodes. Results: Median overall survival was 30 months for all patients. Of the 38 patients with N0 disease, 11 (28.9%) had neck recurrence. Of the 11 neck failures, 9 were ipsilateral only, 1 was contralateral, and 1 had bilateral neck recurrence. The most common site of neck failure was in the upper neck (suhmandibular and jugulodigastric lymph nodes). Four of the 38 patients (10.5%) had isolated neck failure. Only tumor stage was found to be significant for neck relapse, with T1 and T2 doing worse compared to T3 and T4 tumors. Location of tumor (infrastructure vs. suprastructure), involvement of the oral cavity/oropharynx, nasal cavity, nasopharynx or orbit did not predict for cervical node relapse. Local control at the primary site was likewise not prognostic. The median overall survival for patients who remained N0 was 80 months and for those with initial cervical involvement or recurred in the neck without elective neck irradiation was 25 months (p = 0.05). Conclusion: Based on the 28.9 % rate of neck recurrence and the poor median survival of patients who recur in the neck, we recommend prophylactic ipsilateral neck irradiation in patients with T1-T4 squamous cell carcinoma of the maxillary sinus. (C) 1997 Elsevier Science Inc. C1 LOYOLA UNIV,CARDINAL BERNARDIN CANC CTR,MAYWOOD,IL 60153. US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,HINES,IL 60141. RP Paulino, AC (reprint author), LOYOLA UNIV,MED CTR,DEPT RADIOTHERAPY,2160 S 1ST AVE,MAYWOOD,IL 60153, USA. NR 25 TC 59 Z9 59 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0360-3016 J9 INT J RADIAT ONCOL JI Int. J. Radiat. Oncol. Biol. Phys. PD SEP 1 PY 1997 VL 39 IS 2 BP 283 EP 289 DI 10.1016/S0360-3016(97)00293-9 PG 7 WC Oncology; Radiology, Nuclear Medicine & Medical Imaging SC Oncology; Radiology, Nuclear Medicine & Medical Imaging GA XV969 UT WOS:A1997XV96900003 PM 9308929 ER PT J AU Ubel, PA Asch, DA AF Ubel, PA Asch, DA TI Semantic and moral debates about hastening death: A survey of bioethicists SO JOURNAL OF CLINICAL ETHICS LA English DT Article ID PHYSICIAN-ASSISTED SUICIDE; VOLUNTARY ACTIVE EUTHANASIA; LIFE-SUSTAINING TREATMENT; PASSIVE EUTHANASIA; ATTITUDES; OREGON C1 Vet Affairs Med Ctr, Philadelphia, PA 19104 USA. Univ Penn, Div Gen Internal Med, Philadelphia, PA 19104 USA. Univ Penn, Ctr Bioeth, Philadelphia, PA 19104 USA. RP Ubel, PA (reprint author), Vet Affairs Med Ctr, Philadelphia, PA 19104 USA. NR 30 TC 5 Z9 5 U1 0 U2 0 PU UNIV PUBL GROUP, INC PI FREDERICK PA 12 SOUTH MARKET ST, STE 301, FREDERICK, MD 21701 USA SN 1046-7890 J9 J CLIN ETHIC JI J. Clin. Ethics PD FAL PY 1997 VL 8 IS 3 BP 242 EP 249 PG 8 WC Ethics; Social Sciences, Biomedical SC Social Sciences - Other Topics; Biomedical Social Sciences GA YM589 UT WOS:000071079900004 PM 9436082 ER PT J AU Seensalu, R Avedian, D Barbuti, R Song, M Slice, L Walsh, JH AF Seensalu, R Avedian, D Barbuti, R Song, M Slice, L Walsh, JH TI Bombesin-induced gastrin release from canine G cells is stimulated by Ca2+ but not by protein kinase C, and is enhanced by disruption of rho/cytoskeletal pathways SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE bombesin; calcium; protein kinase C; rho factor; cytoskeleton ID SWISS 3T3 CELLS; FOCAL ADHESION KINASE; RHO GENE-PRODUCT; ANTRAL G-CELLS; TYROSINE PHOSPHORYLATION; PRIMARY CULTURE; GROWTH-FACTOR; PAXILLIN; SECRETION; CALCIUM AB Isolated canine G cells in primary culture have been used to study calcium, protein kinase C (PKC), and rho/cytoskeletal-dependent intracellular pathways involved in bombesin-stimulated gastrin release. A method to obtain highly purified G cells by culture (64% G cells) after now cytometry on elutriated fractions of cells from digested canine gastric antral mucosa has been developed. Pretreatment of G cells with thapsigargin (10(-8)-10(-6) M) and release experiments in Ca2+-containing or -depleted media showed that influx of Ca2+ into the cells and not acute release from intracellular stores plays an important role in bombesin-stimulated gastrin release. Inhibition of PKC by the specific inhibitor GF 109 203X did not affect bombesin-stimulated release. Rho, a small GTP-binding protein that regulates the actin cytoskeleton, is specifically antagonized by Clostridium botulinum C3 exoenzyme, C3 (10 mu g/ml) enhanced basal and bombesin-stimulated gastrin release by 315 and 266%, respectively. The importance of the cytoskeleton for regulation of gastrin release was emphasized by a more pronounced release of gastrin when the organization of the actin cytoskeleton was disrupted by cytochalasin D (5 x 10(-7) and 10(-6) M). Wortmannin, a potent inhibitor of phosphoinositide-3-kinase, did not alter bombesin-stimulated gastrin release, Thus, it is concluded that bombesin-induced gastrin release from canine G cells is stimulated by Ca2+ but not by PKC, and is enhanced by disruption of rho/cytoskeletal pathways. C1 W LOS ANGELES VET AFFAIRS MED CTR,CURE,DIGEST DIS RES CTR,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,CURE,DIGEST DIS RES CTR,DEPT MED,LOS ANGELES,CA 90024. RI Barbuti, Ricardo /H-6277-2015 FU NIDDK NIH HHS [DK-41301, DK-17294] NR 41 TC 16 Z9 16 U1 0 U2 0 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD SEP 1 PY 1997 VL 100 IS 5 BP 1037 EP 1046 DI 10.1172/JCI119614 PG 10 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA XV753 UT WOS:A1997XV75300011 PM 9276720 ER PT J AU Anderson, CA Rubinstein, D Filley, CM Stears, JC AF Anderson, CA Rubinstein, D Filley, CM Stears, JC TI MR enhancing brain lesions in methanol intoxication SO JOURNAL OF COMPUTER ASSISTED TOMOGRAPHY LA English DT Article DE poisons and poisoning; alcohol, abuse; brain, abnormalities; magnet resonance imaging ID COMPUTED-TOMOGRAPHY; CT; NECROSIS; TOXICITY AB Methanol intoxication can cause necrosis of the putamen and subcortical white matter that is evident on neuroimaging. We report a 47-year-old man with significant methanol intoxication who had enhancing lesions in the caudate nuclei, putamina, hypothalamus, and subcortical white matter by MRI. This case demonstrates that contrast enhancement of brain lesions can be observed after methanol poisoning. C1 UNIV COLORADO,HLTH SCI CTR,DEPT RADIOL,DENVER,CO 80262. UNIV COLORADO,HLTH SCI CTR,DEPT PSYCHIAT,DENVER,CO 80262. DENVER VET AFFAIRS MED CTR,DENVER,CO. COLORADO MENTAL HLTH INST,PUEBLA,MEXICO. RP Anderson, CA (reprint author), UNIV COLORADO,HLTH SCI CTR,DEPT NEUROL,4200 E 9TH AVE,DENVER,CO 80262, USA. NR 23 TC 28 Z9 29 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0363-8715 J9 J COMPUT ASSIST TOMO JI J. Comput. Assist. Tomogr. PD SEP-OCT PY 1997 VL 21 IS 5 BP 834 EP 836 DI 10.1097/00004728-199709000-00034 PG 3 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA XT931 UT WOS:A1997XT93100035 PM 9294585 ER PT J AU Bogdan, I Leib, SL Bergeron, M Chow, L Tauber, MG AF Bogdan, I Leib, SL Bergeron, M Chow, L Tauber, MG TI Tumor necrosis factor-alpha contributes to apoptosis in hippocampal neurons during experimental group B streptococcal meningitis SO JOURNAL OF INFECTIOUS DISEASES LA English DT Article; Proceedings Paper CT Antibody Workshop - The Role of Humoral Immunity in the Treatment and Prevention of Emerging and Extant Infectious Diseases CY JUN 02-04, 1996 CL WASHINGTON, DC SP Fogarty Int Ctr, NIH, NIAID, NICHD, NCI, US FDA ID BACTERIAL-MENINGITIS; CEREBROSPINAL-FLUID; BRAIN INJURY; RAT MODEL; ACTIVATION; ANTIBODY; CULTURES; INFANTS; INSULTS; CELLS AB To evaluate the role of tumor necrosis factor-alpha (TNF-alpha) in neuronal injury in experimental group B streptococcal meningitis, infected neonatal rats were treated with a monoclonal antibody against TNF-alpha (20 mg/kg intraperitoneally) or saline given at the time of infection. Histopathology after 24 h showed necrosis in the cortex and apoptosis in the hippocampal dentate gyrus. Treated animals had significantly less hippocampal injury than did controls (P < .001) but had similar cortical injury and cerebrospinal fluid (CSF) inflammation. The antibody was then administered directly intracisternally (170 mu g) to test whether higher CSF concentrations reduced inflammation or cortical injury. Again, hippocampal apoptosis was significantly reduced (P < .01), while cortical injury and inflammation were not. Thus, TNF-alpha played a critical role in neuronal apoptosis in the hippocampus, while it was not essential for the development of inflammation and cortical injury in this model. C1 SAN FRANCISCO GEN HOSP,SAN FRANCISCO VA MED CTR,DEPT NEUROL,DIV INFECT DIS,SAN FRANCISCO,CA 94110. UNIV CALIF SAN FRANCISCO,DEPT MED,SAN FRANCISCO,CA. UNIV CALIF SAN FRANCISCO,DEPT NEUROL,SAN FRANCISCO,CA 94143. FU NINDS NIH HHS [NS-32553, NS-34028] NR 28 TC 72 Z9 76 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0022-1899 J9 J INFECT DIS JI J. Infect. Dis. PD SEP PY 1997 VL 176 IS 3 BP 693 EP 697 PG 5 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA XT819 UT WOS:A1997XT81900020 PM 9291317 ER PT J AU Nagra, RM Becher, B Tourtellotte, WW Antel, JP Gold, D Paladino, T Smith, RA Nelson, JR Reynolds, WF AF Nagra, RM Becher, B Tourtellotte, WW Antel, JP Gold, D Paladino, T Smith, RA Nelson, JR Reynolds, WF TI Immunohistochemical and genetic evidence of myeloperoxidase involvement in multiple sclerosis SO JOURNAL OF NEUROIMMUNOLOGY LA English DT Article DE multiple sclerosis; myeloperoxidase; microglia; macrophages; Alu hormone response elements ID CENTRAL-NERVOUS-SYSTEM; MYELIN BASIC-PROTEIN; HUMAN-BONE MARROW; MYELOID DIFFERENTIATION; EXPRESSION; CELLS; AGGREGATION; NEUTROPHILS; ACTIVATION; LEUKOCYTES AB The myeloperoxidase enzyme (MPG) is expressed specifically in myeloid cells and catalyzes the formation of hypochlorous acid and other cytotoxic oxidants. We previously reported that two alleles of MPO exist which differ in promoter strength due to a base difference in an Alu-encoded hormone response element. The present study shows that the higher expressing MPO genotype is overrepresented in early onset multiple sclerosis in females, implicating MPO in this demyelinating disease. Contrary to the general conception that macrophages lack MPG, immunohistochemical analysis shows that MPO is present in microglia/macrophages in and around MS lesions as shown by colocalization with major histocompatibility antigens HLA-DR and phagocytized myelin. Also, MPO mRNA sequences are detected in cDNA derived from isolated human adult microglia. This is the first evidence that MPO is present in microglia/macrophages at MS lesions, that MPO gene expression occurs in microglia and that MPO plays a role in MS pathogenesis as shown by the allelic disequilibrium in early onset disease. (C) 1997 Elsevier Science B.V. C1 SIDNEY KIMMEL CANC CTR,SAN DIEGO,CA 92121. W LOS ANGELES VET AFFAIRS MED CTR,NEUROL RES SERV,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,MED CTR,BRAIN RES INST,LOS ANGELES,CA 90073. MCGILL UNIV,DEPT NEUROL & NEUROSURG,NEUROIMMUNOL UNIT,MONTREAL,PQ H3A 2B4,CANADA. UNIV CALIF SAN DIEGO,SCH MED,DEPT PATHOL,LA JOLLA,CA 92093. CTR NEUROL STUDIES,SAN DIEGO,CA 92121. OI Becher, Burkhard/0000-0002-1541-7867 FU NCRR NIH HHS [RR09118-11] NR 43 TC 176 Z9 178 U1 0 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-5728 J9 J NEUROIMMUNOL JI J. Neuroimmunol. PD SEP PY 1997 VL 78 IS 1-2 BP 97 EP 107 DI 10.1016/S0165-5728(97)00089-1 PG 11 WC Immunology; Neurosciences SC Immunology; Neurosciences & Neurology GA XV851 UT WOS:A1997XV85100011 PM 9307233 ER PT J AU Newton, TF Leuchter, AF van Gorp, WG Hinkin, CH Mandelkern, M Weiner, H AF Newton, TF Leuchter, AF van Gorp, WG Hinkin, CH Mandelkern, M Weiner, H TI EEG power correlates with subcortical metabolic activity in AIDS SO JOURNAL OF NEUROPSYCHIATRY AND CLINICAL NEUROSCIENCES LA English DT Article ID DEMENTIA COMPLEX; HIV-1 INFECTION; COHERENCE; ELECTROENCEPHALOGRAPHY; ZIDOVUDINE; DIAGNOSIS; MEN AB The authors studied the relationship between brain metabolic activity and quantitative electroencephalographic power in AIDS. Basal ganglia and thalamic metabolic activity, measured with positron emission tomography, correlated positively with EEG power in the 6-10-Hz band across most head regions. Metabolic activity of anatomically defined cortical regions did not correlate with EEG power recorded over each region. These results support previously reported associations between abnormalities in subcortical metabolic activity and EEG activity. The lack of correlation between cortical metabolic activity and EEG activity suggests that previously observed abnormalities in EEG activity are primarily subcortical in origin. C1 W Los Angeles Vet Affairs Med Ctr, Dept Psychiat W116AC, Los Angeles, CA 90073 USA. Univ Calif Los Angeles, Neuropsychiat Inst & Hosp, Dept Psychiat & Biobehav Sci, Los Angeles, CA 90024 USA. Univ Calif Irvine, Dept Phys, Irvine, CA 92717 USA. RP Newton, TF (reprint author), W Los Angeles Vet Affairs Med Ctr, Dept Psychiat W116AC, 11301 Wilshire Blvd, Los Angeles, CA 90073 USA. OI newton, thomas/0000-0002-3198-5901 FU NIMH NIH HHS [5T32 MH19200-2] NR 25 TC 6 Z9 7 U1 0 U2 0 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 USA SN 0895-0172 J9 J NEUROPSYCH CLIN N JI J. Neuropsychiatr. Clin. Neurosci. PD FAL PY 1997 VL 9 IS 4 BP 574 EP 578 PG 5 WC Clinical Neurology; Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA YP400 UT WOS:000071273000008 PM 9447499 ER PT J AU Brosnan, J Roper, JM AF Brosnan, J Roper, JM TI The reality of political ethical conflicts - Nurse manager dilemmas SO JOURNAL OF NURSING ADMINISTRATION LA English DT Article RP Brosnan, J (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 5 TC 4 Z9 4 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0002-0443 J9 J NURS ADMIN JI J. Nurs. Adm. PD SEP PY 1997 VL 27 IS 9 BP 42 EP 46 DI 10.1097/00005110-199709000-00010 PG 5 WC Nursing SC Nursing GA XV743 UT WOS:A1997XV74300013 PM 9300014 ER PT J AU Navder, KP Baraona, E Lieber, CS AF Navder, KP Baraona, E Lieber, CS TI Polyenylphosphatidylcholine attenuates alcohol-induced fatty liver and hyperlipemia in rats SO JOURNAL OF NUTRITION LA English DT Article; Proceedings Paper CT 1996 Digestive Disease Week Meeting CY MAY 19-22, 1996 CL SAN FRANCISCO, CA SP Amer Gastroenterol Assoc DE polyenylphosphatidylcholine; alcohol; fatty liver; hyperlipemia; rats ID CHRONIC ETHANOL-CONSUMPTION; HEPATIC MITOCHONDRIA; PATHOGENESIS; ACETALDEHYDE; TOXICITY; FIBROSIS; BABOON; INJURY; OXIDATION; CIRRHOSIS AB Chronic administration of a soybean-derived polyenylphosphatidylcholine (PPC) extract prevents the development of cirrhosis in alcohol-fed baboons. To assess whether this phospholipid also affects earlier changes induced by alcohol consumption (such as fatty liver and hyperlipemia), 28 male rat littermates were pair-fed liquid diets containing 36% of energy either as ethanol or as additional carbohydrate for 21 d, and killed 90 min after intragastric administration of the corresponding diets, Half of the rats were given PPC (3 g/l), whereas the other half received the same amount of linoleate (as safflower oil) and choline (as bitartrate salt), PPC did not affect diet or alcohol consumption [15.4 +/- 0.5 G/(kg.d)], but the ethanol-induced hepatomegaly and the hepatic accumulation of lipids (principally triglycerides and cholesterol esters) and proteins were about half those in rats not given PPC. The ethanol-induced postprandial hyperlipemia was lower with PPC than without, despite an enhanced fat absorption and no difference in the level of plasma free fatty acids. The attenuation of fatty liver and hyperlipemia was associated with correction of the ethanol-induced inhibition of mitochondrial oxidation of palmitoyl-1-carnitine and the depression of cytochrome oxidase activity, as well as the increases in activity of serum glutamate dehydrogenase and aminotransferases. Thus, PPC attenuates early manifestations of alcohol toxicity, at least in part, by improving mitochondrial injury, These beneficial effects of PPC at the initial stages of alcoholic liver injury may prevent or delay the progression to more advanced forms of alcoholic liver disease. C1 MT SINAI SCH MED,NEW YORK,NY 10468. BRONX VET AFFAIRS MED CTR,CTR ALCOHOL RES & TREATMENT,NEW YORK,NY 10468. CUNY HUNTER COLL,DEPT NUTR & FOOD SCI,NEW YORK,NY 10021. FU NIAAA NIH HHS [AA05934, AA11115] NR 41 TC 57 Z9 59 U1 0 U2 0 PU AMER INST NUTRITION PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-3166 J9 J NUTR JI J. Nutr. PD SEP PY 1997 VL 127 IS 9 BP 1800 EP 1806 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA XY002 UT WOS:A1997XY00200012 PM 9278563 ER PT J AU Lang, WP Borgnakke, WS Taylor, GW Woolfolk, MW Ronis, DL Nyquist, LV AF Lang, WP Borgnakke, WS Taylor, GW Woolfolk, MW Ronis, DL Nyquist, LV TI Evaluation and use of an index of oral health status SO JOURNAL OF PUBLIC HEALTH DENTISTRY LA English DT Article; Proceedings Paper CT Annual Session of the American-Association-for-Public-Health-Dentistry CY OCT 05-07, 1995 CL LAS VEGAS, NEVADA SP Amer Assoc Publ Hlth Dent DE oral health status; index; construct validation; population studies ID UNITED-STATES; MEDICAL-CARE; BEHAVIORS AB Objectives: The goals of this investigation were (1) to evaluate the Oral Health Status Index in relation to demographic characteristics, socioeconomic status, and preventive behaviors of an adult population; and (2) to understand how individual index components performed as indicators of oral health status compared to the composite index. Methods: The Oral Health Status index (OHSI) was used on a probability sample of adults, aged 18-93 years, living in the Detroit tricounty area. Data were collected on 509 subjects via in-home dental examinations. Bivariate and multivariate analyses were used to compare the OHSI and its components, including decayed, missing, and replaced teeth, free ends, and moderate and severe periodontal disease measures. Results: The mean OHSI score for subjects was 77.3 (SE=1.83) with a range of -8.0 to 100.0. In regression analyses, OHSI scores were positively correlated with subjects' education level, self-rated oral health scores, and frequency of dental checkups and negatively correlated with age, nonwhite race, and smoking. Of the index components, missing teeth performed well as an indicator of oral health status. Missing teeth were positively correlated with age, nonwhite race, and smoking and negatively correlated with education level, self-rated oral health, and use of Medicaid. About 53 percent of Variance in OHSI scores was explained by the multivariate models, compared to 46 percent for missing teeth. Conclusions: Choosing an indicator of oral health status likely will depend upon the characteristics of the population to be studied. As a composite measure of oral health status, the OHSI performed acceptably; however, missing teeth, an index component, also worked well. Continued evaluation of the OHSI is warranted. C1 Univ Michigan, Sch Dent, Dept Periodont Prevent Geriatr, Ann Arbor, MI 48109 USA. Univ Michigan, Inst Social Res, Ann Arbor, MI 48109 USA. Univ Michigan, Sch Nursing, Ann Arbor, MI 48109 USA. US Dept Vet Affairs, Washington, DC USA. RP Lang, WP (reprint author), Univ Michigan, Sch Dent, Dept Periodont Prevent Geriatr, 1011 N Univ, Ann Arbor, MI 48109 USA. FU NIDCR NIH HHS [DE10145] NR 33 TC 19 Z9 20 U1 2 U2 3 PU AAPHD NATIONAL OFFICE PI PORTLAND PA 3760 SW LYLE COURT, PORTLAND, OR 97221 USA SN 0022-4006 J9 J PUBLIC HEALTH DENT JI J. Public Health Dent. PD FAL PY 1997 VL 57 IS 4 BP 233 EP 242 DI 10.1111/j.1752-7325.1997.tb02980.x PG 10 WC Dentistry, Oral Surgery & Medicine; Public, Environmental & Occupational Health SC Dentistry, Oral Surgery & Medicine; Public, Environmental & Occupational Health GA ZF024 UT WOS:000072855100006 PM 9558627 ER PT J AU Ferrari, AJL Rothfuss, S Schumacher, HR AF Ferrari, AJL Rothfuss, S Schumacher, HR TI Dialysis arthropathy: Identification and evaluation of a subset of patients with unexplained inflammatory effusions SO JOURNAL OF RHEUMATOLOGY LA English DT Article DE amyloid; apatite; inflammation ID CHRONIC-RENAL-FAILURE; CHRONIC-HEMODIALYSIS; BETA-2 MICROGLOBULIN; SYNOVIAL-FLUID; URATE CRYSTALS; ARTHRITIS; ALUMINUM; AMYLOIDOSIS; IRON; MANIFESTATIONS AB Objective. Rheumatic disorders have been reported in patients with chronic renal failure treated with hemodialysis. We identified and evaluated 9 patients undergoing hemodialysis with inflammatory joint effusions not explained by known causes such as gout and bacterial infection. Methods. Forty-nine consecutive synovial fluid (SF) analyses on 41 dialysis patients were reviewed. Nine with unexplained inflammatory arthritis were studied in detail. SF analysis included polarized light examination, alizarin red S stain, Congo red stain, cultures, transmission electron microscopy, and electron probe elemental analysis. Results. SF leukocyte counts ranged from 4550 to 36,000/mm(3) with 44-98% neutrophils. No infections were identifiable in these patients. Findings evaluated as possible factors in the joint inflammation included apatite crystals, iron, lipids, amyloid, and difficult to diagnose nonbacterial infections such as hepatitis C. Conclusion. Some highly inflammatory joint effusions in patients undergoing chronic hemodialysis are not due to pyogenic infections and may be attributable to other factors. C1 VET AFFAIRS MED CTR,ARTHRIT IMMUNOL CTR,PHILADELPHIA,PA 19104. UNIV PENN,SCH MED,PHILADELPHIA,PA 19104. FU NCRR NIH HHS [RR 00040] NR 45 TC 7 Z9 8 U1 1 U2 1 PU J RHEUMATOL PUBL CO PI TORONTO PA 920 YONGE ST, SUITE 115, TORONTO ON M4W 3C7, CANADA SN 0315-162X J9 J RHEUMATOL JI J. Rheumatol. PD SEP PY 1997 VL 24 IS 9 BP 1780 EP 1786 PG 7 WC Rheumatology SC Rheumatology GA XU867 UT WOS:A1997XU86700022 PM 9292804 ER PT J AU Cacciola, JS Alterman, AI Fureman, I Parikh, GA Rutherford, MJ AF Cacciola, JS Alterman, AI Fureman, I Parikh, GA Rutherford, MJ TI The use of case vignettes for addiction severity index training SO JOURNAL OF SUBSTANCE ABUSE TREATMENT LA English DT Article DE ASI; Addiction Severity Index; reliability; validity; assessment ID RELIABILITY; VALIDITY AB In an attempt to enhance the reliability of Addiction Severity Index (ASI) interviewer severity ratings (ISRs), we developed a set of eight ASI vignettes, fictionalized narrative case summaries that reproduced the quantitative ASI information in case report format. Each vignette has an ISR answer key, ct consensus ISR of two expert ASI trainers for each problem area. Additionally, for four of the vignettes the rationale for the correct ISRs was operationalized. This report is a description of the ASI vignette packet, its use as a supplement to standard ASI training and the results of a pilot study to gauge its effectiveness in improving criterion validity of ISRs. Five ASI videotapes were also developed for the purposes of this investigation. There tvas limited evidence in this preliminary investigation that the addition of the ASI vignette packet to standard ASI training increased agreement with expert consensus ISRs. The ASI vignettes, relative to videotaped or live observed interviews, do however provide a brief means of assessing the adequacy of ASI interviewer skills with regard to ISRs. Published by Elsevier Science Inc. C1 Univ Penn, TRC, Ctr Studies Addict, Dept Psychiat, Philadelphia, PA 19104 USA. Vet Affairs Med Ctr, Philadelphia, PA USA. RP Cacciola, JS (reprint author), Univ Penn, TRC, Ctr Studies Addict, Dept Psychiat, 3900 Chestnut St, Philadelphia, PA 19104 USA. NR 8 TC 6 Z9 6 U1 1 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0740-5472 J9 J SUBST ABUSE TREAT JI J. Subst. Abus. Treat. PD SEP-OCT PY 1997 VL 14 IS 5 BP 439 EP 443 DI 10.1016/S0740-5472(97)00123-2 PG 5 WC Psychology, Clinical; Substance Abuse SC Psychology; Substance Abuse GA YN993 UT WOS:000071230600004 PM 9437613 ER PT J AU Ghusn, HF Teasdale, TA Boyer, K AF Ghusn, HF Teasdale, TA Boyer, K TI Characteristics of patients receiving or forgoing resuscitation at the time of cardiopulmonary arrest SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Article; Proceedings Paper CT 48th Gerontological-Society Meeting CY NOV 15-19, 1995 CL LOS ANGELES, CA SP Gerontol Soc ID NURSING-HOMES; SURVIVAL; OUTCOMES; CPR AB OBJECTIVE: To compare clinical, functional and social characteristics of DNR patients at the time of their cardiopulmonary arrest with characteristics of patients who receive cardiopulmonary resuscitation. DESIGN: Retrospective chart review of all 261 patients who had a cardiopulmonary arrest during a 6-month period in an academic institution. SETTING: Teaching Veterans Affairs Medical Center serving a large metropolitan area. MEASUREMENTS: Demographic characteristics, medical diagnoses, and measures of functional status were collected when DNR orders were initiated and at the time of cardiopulmonary arrest. RESULTS: The mean age of the studied group was 62 years. Ninety-nine percent were males, and the majority were non-Hispanic white men. One hundred ninety-eight (76%) patients/proxies elected for limiting treatment. Most (85%) elected a DNR order only. Patients were the most frequently documented participants in advance directive decisions in the DNR group. At the time of cardiopulmonary arrest, a higher proportion of the CPR group had coronary artery disease or chronic renal failure, and a higher proportion of the DNR group had cancer or AIDS. The functional status of the DNR group deteriorated from the time of DNR order to death. At the time of cardiopulmonary arrest, the majority of both groups were dependent in all functional domains, and 70% of the DNR group were stuporous or comatose compared with 47% of the CPR group (P = .05). DNR patients were hospitalized for an average of 13.7 +/- 29.5 days after a DNR order was initiated. Six of the 81 patients who received CPR (7.4%) were alive at discharge. CONCLUSIONS: Patients and physicians deciding to implement a DNR order may be overly focused on medical diagnoses and less so on functional status. A significant proportion of patients with clinical characteristics associated with poor CPR outcome are electing for CPR. C1 BAYLOR COLL MED,HUFFINGTON CTR AGING,HOUSTON,TX 77030. BAYLOR COLL MED,DEPT MED,HOUSTON,TX 77030. RP Ghusn, HF (reprint author), HOUSTON VET AFFAIRS MED CTR,GERIATR & EXTENDED CARE SERV 110,2002 HOLCOMBE BLVD,HOUSTON,TX 77030, USA. NR 17 TC 8 Z9 8 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD SEP PY 1997 VL 45 IS 9 BP 1118 EP 1122 PG 5 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA XU233 UT WOS:A1997XU23300015 PM 9288022 ER PT J AU Gruenewald, DA Gilchriest, JK Anawalt, BD Bremner, WJ Matsumoto, AM AF Gruenewald, DA Gilchriest, JK Anawalt, BD Bremner, WJ Matsumoto, AM TI Development of a clinic for management of benign prostatic hyperplasia in a primary care setting SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract C1 UNIV WASHINGTON,DEPT MED,SEATTLE,WA 98108. VET AFFAIRS PUGET SOUND HLTH CARE SYST,SEATTLE,WA 98108. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD SEP PY 1997 VL 45 IS 9 BP P52 EP P52 PG 1 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA XU233 UT WOS:A1997XU23300125 ER PT J AU Jernberg, JB Mahoney, JE AF Jernberg, JB Mahoney, JE TI The association between strength and function in post-hospitalized elderly patients SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,GRECC,MADISON,WI 53705. UNIV WISCONSIN,MADISON,WI 53705. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD SEP PY 1997 VL 45 IS 9 BP P64 EP P64 PG 1 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA XU233 UT WOS:A1997XU23300137 ER PT J AU Korivi, N Castle, S Wilkins, S Harada, N AF Korivi, N Castle, S Wilkins, S Harada, N TI Relationship among mood, exercise and health in elderly caregivers of dementia patients. SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90024. NR 0 TC 0 Z9 0 U1 1 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD SEP PY 1997 VL 45 IS 9 BP P184 EP P184 PG 1 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA XU233 UT WOS:A1997XU23300257 ER PT J AU MedinaWalpole, AM McCormick, WC AF MedinaWalpole, AM McCormick, WC TI Provider practice patterns in Nursing Home-Acquired Pneumonia SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract C1 UNIV WASHINGTON,DIV GERONTOL & GERIATR MED,DEPT MED,SEATTLE,WA 98104. VA PUGET SOUND HLTH CARE SYST,CTR GERIATR RES EDUC & CLIN,SEATTLE,WA 98104. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD SEP PY 1997 VL 45 IS 9 BP P14 EP P14 PG 1 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA XU233 UT WOS:A1997XU23300087 ER PT J AU Roche, VML Hester, E Welsh, CH MaWhinney, S Shroyer, LS Hammermeister, KE Grover, FL Kramer, A AF Roche, VML Hester, E Welsh, CH MaWhinney, S Shroyer, LS Hammermeister, KE Grover, FL Kramer, A TI Social support major life events and length of stay for coronary artery bypass graft (CABG) patients. SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract C1 UNIV COLORADO,HLTH SCI CTR,DENVER VA MED CTR,DENVER,CO 80220. RI Shroyer, Annie Laurie/B-8836-2016 OI Shroyer, Annie Laurie/0000-0001-6461-0623 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD SEP PY 1997 VL 45 IS 9 BP P308 EP P308 PG 1 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA XU233 UT WOS:A1997XU23300383 ER PT J AU Tan, G Ghusn, H Russell, H Artymiak, K Kelly, J Calhoun, W Thornby, J Teasdale, T AF Tan, G Ghusn, H Russell, H Artymiak, K Kelly, J Calhoun, W Thornby, J Teasdale, T TI Brain stimulation improves cognition and mood of patients with dementia. SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract C1 BAYLOR COLL MED,HUFFINGTON CTR AGING,HOUSTON,TX 77030. HOUSTON VAMC,HOUSTON,TX 77030. NR 0 TC 0 Z9 0 U1 1 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD SEP PY 1997 VL 45 IS 9 BP P233 EP P233 PG 1 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA XU233 UT WOS:A1997XU23300309 ER PT J AU Cohen, DM Zhang, Z Soltoff, SP AF Cohen, DM Zhang, Z Soltoff, SP TI Urea activates PI3K and its effector, P70 S6 kinase, in renal medullary cells. SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 OREGON HLTH SCI UNIV,PORTLAND,OR 97201. PORTLAND VA MED CTR,PORTLAND,OR. HARVARD UNIV,SCH MED,BOSTON,MA. BETH ISRAEL HOSP,BOSTON,MA 02215. RI Zhang, Zheng/J-2388-2014 OI Zhang, Zheng/0000-0003-2497-0362 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1997 VL 8 SU S BP A0238 EP A0238 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA XY103 UT WOS:A1997XY10300238 ER PT J AU Fitzgibbon, WR Ploth, DW AF Fitzgibbon, WR Ploth, DW TI Luminal injection of a bradykinin B-2 receptor antagonist (HOE140) increases distal nephron Na-22 absorption in rats. SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 MED UNIV S CAROLINA,DIV NEPHROL,CHARLESTON,SC 29425. RALPH H JOHNSON VAMC,CHARLESTON,SC. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1997 VL 8 SU S BP A0158 EP A0158 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA XY103 UT WOS:A1997XY10300158 ER PT J AU Frazao, JM Coburn, JW Chesney, RW AF Frazao, JM Coburn, JW Chesney, RW TI Use of one-alpha-hydroxyvitamin D-2 (1 alpha D-2) in 121 hemodialysis patients with secondary hyperparathyroidism: A phase 3 trial. SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 UNIV TENNESSEE,MEMPHIS,TN. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,LOS ANGELES,CA. BONE CARE INT INC,MADISON,WI. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1997 VL 8 SU S BP A2671 EP A2671 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA XY103 UT WOS:A1997XY10302670 ER PT J AU Ghosh, PM Mott, GE Stapleton, ML GhoshChoudhury, N Kreisberg, JI AF Ghosh, PM Mott, GE Stapleton, ML GhoshChoudhury, N Kreisberg, JI TI Lovastatin causes apoptosis in a mouse prostate cancer cell by inhibiting mitotic and post-mitotic events. SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 S TEXAS VET HLTH CARE SYST,RES & DEV,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1997 VL 8 SU S BP A1956 EP A1956 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA XY103 UT WOS:A1997XY10301956 ER PT J AU Kher, R Nagami, GT Bacallao, RL AF Kher, R Nagami, GT Bacallao, RL TI Cloning of a putative receptor tyrosine kinase from a kidney cell line using differential display polymerase chain reaction (DD-PCR). SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 ROUDEBUSCH VA MED CTR,DIV NEPHROL,INDIANAPOLIS,IN. INDIANA UNIV,SCH MED,INDIANAPOLIS,IN. W LOS ANGELES VAMC,NEPHROL SECT,MED & RES SVCS,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1997 VL 8 SU S BP A1663 EP A1663 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA XY103 UT WOS:A1997XY10301663 ER PT J AU Kroning, R Lichtenstein, AK Nagami, GT AF Kroning, R Lichtenstein, AK Nagami, GT TI Differential inhibition of cisplatin toxicity and uptake in cultured S1, S3 and DCT cells by L-cysteine. SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,DIV NEPHROL,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,DEPT MED,DIV HEMATOL ONCOL,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA 90024. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1997 VL 8 SU S BP A2815 EP A2815 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA XY103 UT WOS:A1997XY10302813 ER PT J AU Landis, CA Nugent, ME Wang, MM Kraut, J Nagami, G Shinaberger, J AF Landis, CA Nugent, ME Wang, MM Kraut, J Nagami, G Shinaberger, J TI Improved survival with self-care center hemodialysis compared to conventional center hemodialysis: A single center historical study. SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 W LOS ANGELES VAMC,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,LOS ANGELES,CA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1997 VL 8 SU S BP A0927 EP A0927 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA XY103 UT WOS:A1997XY10300927 ER PT J AU Nagami, GT Katz, D Warech, EM AF Nagami, GT Katz, D Warech, EM TI Identification and mRNA expression of the 5' untranslated region of the mouse proximal tubule angiotensin II type 1A receptor. SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,DEPT MED,NEPHROL SECT,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,RES DEPT,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA 90024. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1997 VL 8 SU S BP A0036 EP A0036 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA XY103 UT WOS:A1997XY10300036 ER PT J AU Simon, M Marish, JG Hernandez, JD Olson, M Abboud, HE AF Simon, M Marish, JG Hernandez, JD Olson, M Abboud, HE TI Localization of BMP-7 mRNA in the adult rat kidney: Potential role in ischemia and injury. SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET AFFAIRS HOSP,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT BIOCHEM,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1997 VL 8 SU S BP A2775 EP A2775 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA XY103 UT WOS:A1997XY10302773 ER PT J AU Simon, M Hernandez, JD Cleary, TG Abboud, HE AF Simon, M Hernandez, JD Cleary, TG Abboud, HE TI MCP-1 gene expression induced by shiga-like toxin (SLT-1) in human mesangial cells. SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. UNIV TEXAS,SCH MED,DEPT PEDIAT,HOUSTON,TX. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1997 VL 8 SU S BP A2358 EP A2358 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA XY103 UT WOS:A1997XY10302358 ER PT J AU Ullian, ME Greene, EL Raymond, JR AF Ullian, ME Greene, EL Raymond, JR TI Epidermal growth factor (EGF) downregulates angiotensin II (Ang II) receptors without tyrosine kinase activation. SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 RALPH H JOHNSON VET ADM MED CTR,CHARLESTON,SC. MED UNIV S CAROLINA,CHARLESTON,SC 29425. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1997 VL 8 SU S BP A1894 EP A1894 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA XY103 UT WOS:A1997XY10301894 ER PT J AU Xu, Y Arar, M Bunegin, M Simon, M Barnes, J Elshihabi, I Abboud, H AF Xu, Y Arar, M Bunegin, M Simon, M Barnes, J Elshihabi, I Abboud, H TI Morphological insights into the origin of glomerular mesangial and endothelial cells: Possible role of platelet-derived growth factor receptor (PDGFR-beta) in the developing kidney. SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1997 VL 8 SU S BP A1700 EP A1700 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA XY103 UT WOS:A1997XY10301700 ER PT J AU Jacob, AD Elkins, N Reiss, OK Chan, L Shapiro, JI AF Jacob, AD Elkins, N Reiss, OK Chan, L Shapiro, JI TI Effects of acetate on energy metabolism and function in the isolated perfused rat heart SO KIDNEY INTERNATIONAL LA English DT Article DE energy metabolism; acetate; hypotension during dialysis; cardiac function; vasodilation ID NUCLEAR MAGNETIC-RESONANCE; CONTRACTILE DYSFUNCTION; INTRACELLULAR ACIDOSIS; MYOCARDIAL-FUNCTION; REGIONAL ISCHEMIA; CORONARY FLOW; INVIVO; HEMODIALYSIS; SPECTROSCOPY; BICARBONATE AB Impairment of cardiac contractile function is an important component of acetate associated hypotension during hemodialysis treatments. We examined the effect of acetate on cardiac energy metabolism using the isovolumic isolated perfused heart model. In this preparation, acetate (10 M) caused decreases in tissue ATP concentrations (12.3 +/- 0.8 vs. 15.6 +/- 1.0 mu mol/g dry at 30 min, P < 0.05) as well as marked impairment of systolic function (dpdt = 863 +/- 135 vs. 1288 +/- 166 mm Hg/second at 30 min, P < 0.05). Although altering perfusate calcium concentrations (0.6, 1.2 and 2.4 mM) affected physiological responses to acetate (5 and 10 mM), the reductions in tissue ATP concentrations were similar. In isolated heart mitochondria, acetate (100 mu M -10 mM) selectively impaired octanoate and palmityl carnitine supported State 3 respiration in a dose dependent fashion (P < 0.01), but did not affect respiration when succinate, pyruvate/malate or malate-glutamate was used as substrate. We suggest that high concentrations of acetate selectively impair fatty acid metabolism in heart issue. This in turn leads to decreases in ATP production and tissue ATP concentrations that ultimately result in impaired contractile function. As this occurs at relatively low concentrations of acetate, this finding may be relevant to other parenterally-administered acetate containing fluids. C1 UNIV COLORADO,SCH MED,GILES FILLEY RES LAB,WEBB WARING INST BIOMED RES,BOULDER,CO 80309. UNIV COLORADO,SCH MED,DENVER VA MED CTR,DEPT MED,BOULDER,CO 80309. UNIV COLORADO,SCH MED,DENVER VA MED CTR,DEPT RADIOL,BOULDER,CO 80309. NR 33 TC 15 Z9 16 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 SN 0085-2538 J9 KIDNEY INT JI Kidney Int. PD SEP PY 1997 VL 52 IS 3 BP 755 EP 760 DI 10.1038/ki.1997.392 PG 6 WC Urology & Nephrology SC Urology & Nephrology GA XT549 UT WOS:A1997XT54900022 PM 9291197 ER PT J AU Abboud, HE AF Abboud, HE TI Growth factors and diabetic nephropathy: An overview SO KIDNEY INTERNATIONAL LA English DT Article; Proceedings Paper CT 3rd Hyonam-Kidney-Laboratory International Symposium on Pathogenesis of Diabetic Nephropathy - Experimental Approaches, an Update CY JAN 24-25, 1997 CL SEOUL, SOUTH KOREA SP Hyonam Kidney Lab DE cytokines; growth factors; diabetes; hemodynamics ID NECROSIS-FACTOR-ALPHA; GENE-EXPRESSION; MESANGIAL CELLS; KIDNEY-DISEASE; MESSENGER-RNA; END-PRODUCTS; FACTOR-BETA; RAT-KIDNEY; RECEPTOR; HYPERTROPHY C1 S TEXAS VET HLTH CARE SYST,AUDIE L MURPHY DIV,SAN ANTONIO,TX. RP Abboud, HE (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV NEPHROL,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. NR 35 TC 3 Z9 3 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 SN 0085-2538 J9 KIDNEY INT JI Kidney Int. PD SEP PY 1997 SU 60 BP S3 EP S6 PG 4 WC Urology & Nephrology SC Urology & Nephrology GA XU763 UT WOS:A1997XU76300002 ER PT J AU Asch, DA DeKay, ML AF Asch, DA DeKay, ML TI Euthanasia among US critical care nurses - Practices, attitudes, and social and professional correlates SO MEDICAL CARE LA English DT Article DE attitudes; behaviors; decision-making; ethics; euthanasia; life-support; nursing; survey research ID LIFE-SUSTAINING TREATMENTS; ASSISTED SUICIDE; PHYSICIAN ATTITUDES; ACTIVE EUTHANASIA; DECISIONS; WITHDRAW; SUPPORT; PREFER AB OBJECTIVES. The authors sought to identify associations between critical care nurses' self-reported participation in euthanasia, their social and professional characteristics, and their attitudes toward end-of-life care. METHODS. Data were collected through an anonymous mail survey of 1,560 US critical care nurses, of whom 1,139 (73%) responded. Nurses were asked to report whether they had received requests to engage in euthanasia and whether they had engaged in euthanasia. In addition, nurses were asked to respond to items assessing their attitudes toward end-of-life care. RESULTS. Of 852 nurses who identified themselves as practicing exclusively in adult intensive care units, 164 (19%) reported that they had engaged in euthanasia, 650 (76%) reported that they had not engaged in euthanasia, and 38 (4%) could not be classified. Only 30% of respondents believed that euthanasia is unethical. Logistic regression indicated that older nurses, more religious nurses, nurses practicing in cardiac care units, and nurses with less favorable attitudes toward euthanasia were significantly less likely to report having engaged in euthanasia, although the effects of age and religious beliefs appear to have been mediated by attitudes. CONCLUSIONS. These results help explain why some US critical care nurses engaged in euthanasia despite legal and professional prohibitions against it. Because critical care nurses may have a special understanding of the needs of critically ill patients, these results may indicate that current guidelines for end-of-life care are inadequate. C1 VET AFFAIRS MED CTR,PHILADELPHIA,PA. UNIV PENN,SCH MED,CTR BIOETH,PHILADELPHIA,PA 19104. UNIV PENN,LEONARD DAVIS INST HLTH ECON,PHILADELPHIA,PA 19104. UNIV PENN,WHARTON SCH,DEPT OPERAT & INFORMAT MANAGEMENT,PHILADELPHIA,PA 19104. RP Asch, DA (reprint author), UNIV PENN,SCH MED,DIV GEN INTERNAL MED,RALSTON PENN CTR 317,3615 CHESTNUT ST,PHILADELPHIA,PA 19104, USA. NR 26 TC 23 Z9 23 U1 3 U2 8 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0025-7079 J9 MED CARE JI Med. Care PD SEP PY 1997 VL 35 IS 9 BP 890 EP 900 PG 11 WC Health Care Sciences & Services; Health Policy & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA XV885 UT WOS:A1997XV88500002 PM 9298078 ER PT J AU Wray, NP Hollingsworth, JC Petersen, NJ Ashton, CM AF Wray, NP Hollingsworth, JC Petersen, NJ Ashton, CM TI Case-mix adjustment using administrative databases: A paradigm to guide future research SO MEDICAL CARE RESEARCH AND REVIEW LA English DT Review ID ACUTE MYOCARDIAL-INFARCTION; CLINICAL COMORBIDITY INDEX; PROSPECTIVE-PAYMENT SYSTEM; CONGESTIVE-HEART-FAILURE; ACADEMIC-MEDICAL-CENTER; IN-HOSPITAL MORTALITY; MARITAL-STATUS; RISK ADJUSTMENT; SOCIAL-CLASS; PATIENT CHARACTERISTICS AB One of the most persistent problems in the field of quality assessment remains how to remove the confounding effect of different institutions providing care to patients with dissimilar severity of illness and case complexity. The authors review the literature to determine whether risk adjust ment systems based on administrative data are inherently inferior to systems that depend on primary data collection and conclude that they are not. In light of the potential competence of risk adjustment systems based on administrative data, the authors identify those systems that are best supported by theory and evidence. Data elements that have been found most explanatory of medical outcomes are also identified. On the basis of an evaluation of the performance of various risk adjustment approaches, the authors propose a paradigm that could serve to unify and direct future studies. RP Wray, NP (reprint author), BAYLOR COLL MED,HOUSTON VA MED CTR,HOUSTON,TX 77030, USA. NR 104 TC 34 Z9 35 U1 4 U2 5 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 SN 1077-5587 J9 MED CARE RES REV JI Med. Care Res. Rev. PD SEP PY 1997 VL 54 IS 3 BP 326 EP 356 DI 10.1177/107755879705400306 PG 31 WC Health Care Sciences & Services; Health Policy & Services SC Health Care Sciences & Services GA XU944 UT WOS:A1997XU94400006 PM 9437171 ER PT J AU Leaf, DA Parker, DL Schaad, D AF Leaf, DA Parker, DL Schaad, D TI Changes in VO2max, physical activity, and body fat with chronic exercise: Effects on plasma lipids SO MEDICINE AND SCIENCE IN SPORTS AND EXERCISE LA English DT Article DE exercise; physical activity; lipids; coronary artery disease ID DENSITY-LIPOPROTEIN CHOLESTEROL; ALL-CAUSE MORTALITY; MIDDLE-AGED MEN; SKELETAL-MUSCLE; ADIPOSE-TISSUE; WEIGHT-LOSS; FITNESS; LIPASE; SEDENTARY; HEALTHY AB 0The effect of changes in physical activity levels during chronic exercise on plasma lipids and lipoproteins has not been reported. We examine the relationships between changes in VO2max, leisure time physical activity (LTPA), and percent body fat on changes in plasma lipids and lipoproteins in 137 men without coronary artery disease (CAD) and/or dyslipidemia, hypertension, or diabetes mellitus who participated in an employee exercise program. Measurements obtained at (sic) entry (sic) and 1- and 4-yr follow-up include VO2max, LTPA in kcal.wk(-1), percent body fat, and plasma lipids and lipoproteins. The relationship between changes in the measurements between 1 and 4 yr of follow-up (N = 34) revealed the following significant (P < 0.05) correlations: i) changes in VO2max with changes in percent body fat (r = -0.289) and changes in plasma triglycerides (r = -0.354), ii) changes in LTPA with changes in percent body fat (-0.361), and iii) changes in percent body fat with changes in the total/high density lipoprotein (HDL)-cholesterol ratio (0.358), HDL-cholesterol (-0.212), and triglycerides (0.289). Multiple regression analysis revealed that changes in percent body fat affected changes in plasma triglycerides (P < 0.05). The effects of chronic physical activity on plasma triglycerides appear to result from exercise-related effects on body adiposity. These findings support the role of regular physical activity as mandated by Healthy People 2000 for CAD risk reduction. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90024. WASHINGTON SPORTS MED INST,KIRKLAND,WA. UNIV WASHINGTON,DEPT MED EDUC,SEATTLE,WA 98195. RP Leaf, DA (reprint author), W LOS ANGELES VA MED CTR,DEPT MED,111G WILSHIRE & SAWTELLE BLVD,LOS ANGELES,CA 90073, USA. FU NHLBI NIH HHS [HL 30564] NR 52 TC 20 Z9 21 U1 3 U2 6 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0195-9131 J9 MED SCI SPORT EXER JI Med. Sci. Sports Exerc. PD SEP PY 1997 VL 29 IS 9 BP 1152 EP 1159 DI 10.1097/00005768-199709000-00006 PG 8 WC Sport Sciences SC Sport Sciences GA XW921 UT WOS:A1997XW92100006 PM 9309625 ER PT J AU Bader, TJ Macones, GA Asch, DA AF Bader, TJ Macones, GA Asch, DA TI Prenatal screening for toxoplasmosis SO OBSTETRICS AND GYNECOLOGY LA English DT Article ID CONGENITAL TOXOPLASMOSIS; AMNIOCENTESIS; DIAGNOSIS; MALFORMATIONS; PREVENTION; INFECTIONS; INFANTS AB Objective: To evaluate the merits of screening for toxoplasmosis in all pregnant women. Methods: We used decision analysis to compare three strategies for the antepartum management of congenital toxoplasmosis: 1) no testing for congenital toxoplasmosis; 2) current practice, which is to perform targeted screening in cases of incidental abnormalities noted on ultrasound; and 3) universal serologic screening of pregnant women followed by amniocentesis to diagnose fetal infection in cases of maternal seroconversion. For each of the three strategies, we considered the two available treatment options: intrauterine antiparasitic treatment or pregnancy termination. Results: Universal screening reduced the total number of cases of congenital toxoplasmosis compared with no testing or targeted screening. However, compared with no testing, universal screening with medical treatment resulted in 18.5 additional pregnancy losses for each case of toxoplasmosis avoided. If infected pregnancies underwent Germination, universal screening resulted in 12.1 additional pregnancy losses for each case avoided. Conclusion: Maternal screening reduces the number of cases of disease, but at a substantial clinical cost. The rarity of the disease and limitations in diagnosis and therapy limit the effectiveness of screening strategies. The risks associated with amniocentesis are particularly important. Universal maternal screening for congenital toxoplasmosis should not be performed. ((C) 1997 by The American College of Obstetricians and Gynecologists). C1 UNIV PENN,SCH MED,CTR CLIN EPIDEMIOL & BIOSTAT,PHILADELPHIA,PA 19104. UNIV PENN,SCH MED,DIV GEN INTERNAL MED,PHILADELPHIA,PA 19104. VET AFFAIRS MED CTR,PHILADELPHIA,PA. RP Bader, TJ (reprint author), UNIV PENN,SCH MED,DEPT OBSTET & GYNECOL,106 DULLES BLDG,3400 SPRUCE ST,PHILADELPHIA,PA 19104, USA. NR 30 TC 27 Z9 28 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0029-7844 J9 OBSTET GYNECOL JI Obstet. Gynecol. PD SEP PY 1997 VL 90 IS 3 BP 457 EP 464 DI 10.1016/S0029-7844(97)00291-3 PG 8 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA XT158 UT WOS:A1997XT15800026 PM 9277662 ER PT J AU Bussey, HI Chiquette, E Bianco, M LowderBender, K Kraynak, MA Linn, WD Farnett, L Clark, GM AF Bussey, HI Chiquette, E Bianco, M LowderBender, K Kraynak, MA Linn, WD Farnett, L Clark, GM TI A statistical and clinical evaluation of fingerstick and routine laboratory prothrombin time measurements SO PHARMACOTHERAPY LA English DT Article; Proceedings Paper CT Winter Practice and Research Forum of the American-College-of-Clinical-Pharmacy CY FEB 12-15, 1994 CL ORLANDO, FL SP Amer Coll Clin Pharm ID INTERNATIONAL NORMALIZED RATIO; ORAL ANTICOAGULANT-THERAPY; RELIABILITY; MONITOR AB Study Objective. To compare prothrombin time measurements by fingerstick and routine laboratory methods. Design. Prospective cohort study. Setting. University-affiliated anticoagulation clinic. Patients. Thirty-three patients receiving warfarin with stable anticoagulation for 3 months preceding the two studies. Interventions. Groups 1 (17 patients) and 2 (16 patients) provided 150 and 125 paired samples, respectively, for fingerstick and routine laboratory analysis. The fact that no patient required a dosage change allowed for a clinical assessment. Measurements and Main Results. Correlation and agreement between methods were good in group 1 but poor in group 2. Fingerstick results were less variable (smaller standard deviation and smaller coefficient of repeatability) in both groups. By analysis of discrepant pairs (25 in group 1, 63 in group 2), the routine laboratory results indicated dosage changes erroneously more often than did the fingerstick method. Conclusions. In these two trials, the fingerstick system was superior to the routine laboratory method in that it was more reliable (less variability and more repeatable) and less likely to indicate dosage changes erroneously. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SERV PHARM,SAN ANTONIO,TX 78284. UNIV TEXAS,COLL PHARM,AUSTIN,TX 78712. RP Bussey, HI (reprint author), UNIV TEXAS,HLTH SCI CTR,DIV PHARMACOTHERAPY,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. NR 16 TC 26 Z9 27 U1 0 U2 0 PU PHARMACOTHERAPY PUBLICATIONS INC PI BOSTON PA NEW ENGLAND MEDICAL CENTER BOX 806 171 HARRISON AVE, BOSTON, MA 02111 SN 0277-0008 J9 PHARMACOTHERAPY JI Pharmacotherapy PD SEP-OCT PY 1997 VL 17 IS 5 BP 861 EP 866 PG 6 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA XX704 UT WOS:A1997XX70400002 PM 9324174 ER PT J AU Streim, JE Oslin, D Katz, IR Parmelee, PA AF Streim, JE Oslin, D Katz, IR Parmelee, PA TI Lessons from geriatric psychiatry in the long term care setting SO PSYCHIATRIC QUARTERLY LA English DT Article ID NURSING-HOME PATIENTS; NEUROLEPTIC TREATMENT; DEPRESSIVE SYMPTOMS; AGITATED BEHAVIORS; MENTAL-DISORDERS; DEMENTIA; FACILITIES; REGULATIONS; MORTALITY; RESIDENTS AB Of all long term care settings, the nursing home has served as the most productive laboratory for the study of the mental health problems of late Life. Lessons from geriatric psychiatry research and practice in the nursing home have relevance to general psychiatry and to other health care settings, informing us about (a) psychiatric disorders in medically ill and disabled populations; (b) subsyndromes and subtypes of depression; (c) behavioral disturbances in patients with brain injury; (d) the effects of government regulation and education on mental health care; and (e) essential roles for psychiatrists in changing health care systems. Selected areas of knowledge based on geriatric psychiatry research and experience in long term care are reviewed in this paper, and their applications for the field of psychiatry in general are explored. C1 HOSP UNIV PENN,PHILADELPHIA,PA 19104. UNIV PENN,PHILADELPHIA VA MED CTR,SECT GERIATR PSYCHIAT,PHILADELPHIA,PA 19104. NR 79 TC 12 Z9 12 U1 2 U2 2 PU HUMAN SCI PRESS INC PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013-1578 SN 0033-2720 J9 PSYCHIAT QUART JI Psychiatr. Q. PD FAL PY 1997 VL 68 IS 3 BP 281 EP 307 DI 10.1023/A:1025440408223 PG 27 WC Psychiatry SC Psychiatry GA XK087 UT WOS:A1997XK08700006 PM 9237321 ER PT J AU Kennefick, TM Anderson, S AF Kennefick, TM Anderson, S TI Role of angiotensin II in diabetic nephropathy SO SEMINARS IN NEPHROLOGY LA English DT Review ID CONVERTING ENZYME-INHIBITION; METABOLIC-CLEARANCE RATE; SMOOTH-MUSCLE CELLS; ANTIHYPERTENSIVE THERAPY; GENE POLYMORPHISM; MESANGIAL CELLS; GROWTH-FACTOR; PROXIMAL TUBULE; RENIN; SYSTEM C1 OREGON HLTH SCI UNIV,DEPT MED,DIV NEPHROL & HYPERTENS,PORTLAND,OR 97201. PORTLAND VA MED CTR,PORTLAND,OR. NR 75 TC 31 Z9 32 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0270-9295 J9 SEMIN NEPHROL JI Semin. Nephrol. PD SEP PY 1997 VL 17 IS 5 BP 441 EP 447 PG 7 WC Urology & Nephrology SC Urology & Nephrology GA XY046 UT WOS:A1997XY04600007 PM 9316212 ER PT J AU Safran, DG Rogers, WH Tarlov, AR McHorney, CA Ware, JE AF Safran, DG Rogers, WH Tarlov, AR McHorney, CA Ware, JE TI Gender differences in medical treatment: The case of physician-prescribed activity restrictions SO SOCIAL SCIENCE & MEDICINE LA English DT Article DE gender; illness behavior; physician-patient interaction ID HEALTH SURVEY SF-36; SEX-DIFFERENCES; MENTAL-HEALTH; UNITED-STATES; CARE; MORTALITY; AGE; OUTCOMES; TRANSPLANTATION; ADMISSION AB A growing scientific literature highlights concern about the influence of social bias in medical care. Differential treatment of male and female patients has been among the documented concerns. Yet, little is known about the extent to which differential treatment of male and female patients reflects the influence of social bias or of more acceptable factors, such as different patient preferences or different anticipated outcomes of care. This paper attempts to ascertain the underlying basis for an observed differential in physicians' tendency to advise activity restrictions for male and female patients. We explore the extent to which the gender-based treatment differential is attributable to: (1) patients' health profile, (2) patients' role responsibilities, (3) patients' illness behaviors, and (4) physician characteristics. These four categories of variables correspond to four prominent social science hypotheses concerning gender differences in health and health care utilization (i.e. biological basis hypothesis, fixed role hypothesis, socialization hypothesis, physician bias hypothesis). Data are drawn from the Medical Outcomes Study (MOS), a longitudinal observational study of 1546 patients of 349 physicians practicing in three U.S. cities. Multivariate logistic regression is used to evaluate the likelihood of physician-prescribed activity restrictions for male and female patients, and to explore the absolute and relative influence of patient and physician factors on the observed treatment differential. Results reveal that the odds of prescribed activity restrictions are 3.6 times higher for female patients than for males with equivalent characteristics. The observed differential is not explained by differences in male and female patients' health or role responsibilities. Gender differences in illness behavior and physician gender biases both appear to contribute to the observed differential. Female patients exhibit more illness behavior than males, and these behaviors increase physicians' tendency to prescribe activity restrictions. After accounting for illness behavior differences and all other factors, the odds of prescribed activity restrictions among female patients of male physicians is four times that of equivalent male patients of those physicians. Medical practice, education, and research must strive to identify and remove the likely unconscious role of social bias in medical decision making. (C) 1997 Elsevier Science Ltd. C1 TUFTS UNIV,DEPT MED,BOSTON,MA 02111. TUFTS UNIV,DEPT PSYCHIAT,BOSTON,MA 02111. HARVARD UNIV,SCH PUBL HLTH,BOSTON,MA 02115. UNIV WISCONSIN,SCH MED,DEPT MED,MADISON,WI. UNIV WISCONSIN,SCH MED,DEPT PREVENT MED,MADISON,WI. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. RP Safran, DG (reprint author), TUFTS UNIV NEW ENGLAND MED CTR,HLTH INST,750 WASHINGTON ST,BOX 345,BOSTON,MA 02111, USA. NR 48 TC 43 Z9 43 U1 2 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0277-9536 J9 SOC SCI MED JI Soc. Sci. Med. PD SEP PY 1997 VL 45 IS 5 BP 711 EP 722 DI 10.1016/S0277-9536(96)00405-4 PG 12 WC Public, Environmental & Occupational Health; Social Sciences, Biomedical SC Public, Environmental & Occupational Health; Biomedical Social Sciences GA XH543 UT WOS:A1997XH54300007 PM 9226794 ER PT J AU Hayden, JL Kern, RS Burdick, NL Green, MF AF Hayden, JL Kern, RS Burdick, NL Green, MF TI Neurocognitive impairments associated with ambiguous handedness in the chronically mentally ill SO PSYCHIATRY RESEARCH LA English DT Article DE schizophrenia; ambiguous handedness; neurocognitive functioning; verbal learning; motor learning; manual dexterity ID PATHOLOGICAL LEFT-HANDEDNESS; THOUGHT-DISORDER; SCHIZOPHRENIA; LANGUAGE AB One form of atypical handedness, ambiguous handedness, is found in roughly one-quarter of chronic schizophrenic patients. Despite its prevalence, relatively little is known about the neurccognitive underpinnings of ambiguous handedness. In the present study we examined the performance of ambiguous (n = 19) and non-ambiguous (n = 39) handed chronically mentally ill inpatients on selected measures of verbal learning, motor learning and manual dexterity. The results revealed that ambiguous handers were more impaired than non-ambiguous handers in verbal learning, but not motor learning. Group differences in manual dexterity were significant for the entire sample, but not when analyses were limited to males. These findings suggest that impairments in verbal learning may be linked to the pathogenesis of ambiguous handedness in chronic psychiatric patients. (C) 1997 Elsevier Science Ireland Ltd. C1 UNIV CALIF LOS ANGELES,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. VENTURA CTY SCH DIST,VENTURA,CA 90073. FU NIMH NIH HHS [MH-30911] NR 19 TC 5 Z9 5 U1 0 U2 1 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0165-1781 J9 PSYCHIAT RES JI Psychiatry Res. PD AUG 29 PY 1997 VL 72 IS 1 BP 9 EP 16 DI 10.1016/S0165-1781(97)00088-7 PG 8 WC Psychiatry SC Psychiatry GA YB640 UT WOS:A1997YB64000002 PM 9355814 ER PT J AU Roitman, SEL Keefe, RSE Harvey, PD Siever, LJ Mohs, RC AF Roitman, SEL Keefe, RSE Harvey, PD Siever, LJ Mohs, RC TI Attentional and eye tracking deficits correlate with negative symptoms in schizophrenia SO SCHIZOPHRENIA RESEARCH LA English DT Article DE schizophrenia; continuous performance test; eye tracking; attention; positive and negative symptoms ID CONTINUOUS PERFORMANCE-TEST; SMOOTH-PURSUIT PERFORMANCE; AFFECTIVE-DISORDERS; REMITTED SCHIZOPHRENICS; SUSTAINED ATTENTION; CLINICAL SYMPTOMS; VULNERABILITY; SCHIZOTYPAL; RELATIVES; SPECIFICITY AB Thirty patients with a DSM-III-R diagnosis of schizophrenia were assessed for severity of schizophrenic symptoms using the Brief Psychiatric Rating Scale (BPRS) and were tested on a Continuous Performance Test (CPT) and a smooth pursuit eye tracking task. Negative symptoms were significantly correlated with eye tracking impairment (r = 0.43, p<0.01) and CPT deficits (r =0.67, p<0.001), but performance on neither task was correlated with positive symptoms. CPT performance and eye tracking performance were modestly correlated with each other (r=0.39, p<0.01) and CPT performance was found to be a stronger predictor of negative symptoms than eye tracking performance. These data indicate that neurocognitive markers of vulnerability to schizophrenia are associated with negative rather than positive symptoms. (C) 1997 Elsevier Science B.V. C1 MT SINAI SCH MED,DEPT PSYCHIAT,NEW YORK,NY 10029. BRONX VET ADM MED CTR,NEW YORK,NY 10468. NR 51 TC 28 Z9 28 U1 1 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD AUG 29 PY 1997 VL 26 IS 2-3 BP 139 EP 146 PG 8 WC Psychiatry SC Psychiatry GA XY358 UT WOS:A1997XY35800008 PM 9323344 ER PT J AU Pahlavani, MA Harris, MD Richardson, A AF Pahlavani, MA Harris, MD Richardson, A TI The increase in the induction of IL-2 expression with caloric restriction is correlated to changes in the transcription factor NFAT SO CELLULAR IMMUNOLOGY LA English DT Article ID ACTIVATED T-CELLS; AGE-RELATED DECLINE; DNA-BINDING ACTIVITY; DIETARY RESTRICTION; NUCLEAR FACTOR; INTERLEUKIN-2 SYNTHESIS; GENE-EXPRESSION; IMMUNE FUNCTION; KAPPA-B; RATS AB The objective of this study was to determine if the increase in the induction of interleukin-2 (IL-2) expression with caloric restriction correlates with changes in binding activity of the IL-a-specific transcription factor NFAT (nuclear factor of activated T cells) and/or the ubiquitous transcription factor AP-1 in T cells from male Fischer 344 rats. Splenic T cells were isolated from young (6-month) and old (24-month) rats fed ad libitum and from old (24-month) rats fed a restricted diet (40% caloric restriction) that began at 6 weeks of age. T cells were stimulated with concanavalin A (Con A) and the expression of IL-2 and the DNA binding activity of the transcription factors NFAT and AP-1 were measured in these cells. We found that the induction of IL-2 activity and mRNA levels decreased with age and that caloric restriction significantly (P < 0.05) reduced the age-related decline in IL-2 expression. The ability of nuclear extracts from T cells isolated from old rats fed ad libitum and restricted old rats to bind to the NFAT oligonucleotide or AP-1 oligonucleotide decreased with age. Caloric restriction significantly (P < 0.05) reduced the age-related decline in NFAT but had no significant effect on AP-1 binding activity. We also measured the induction of c-fos and c-jun expression by Con A in T cells from young and old rats fed ad libitum or caloric-restricted diet. The induction of c-fos protein and mRNA levels but not c-jun protein or mRNA levels decreased significantly with age. Caloric restriction significantly (P < 0.05) reduced the age-related decline in c-fos expression but had no significant effect on c-jun expression. Therefore, the increase in IL-2 expression with caloric restriction correlates with an increase in binding activity of transcription factor NFAT and an increase in the expression of c-fos, which is a component of the NFAT-protein complex. (C) 1997 Academic Press. C1 UNIV TEXAS, HLTH SCI CTR, DEPT PHYSIOL, SAN ANTONIO, TX 78284 USA. RP Pahlavani, MA (reprint author), UNIV TEXAS, HLTH SCI CTR, S TEXAS VET HLTH CARE SYST, CTR GERIATR RES EDUC & CLIN, SAN ANTONIO, TX 78284 USA. FU NIA NIH HHS [AG00677, AG00165, AG01548] NR 57 TC 21 Z9 22 U1 0 U2 0 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0008-8749 J9 CELL IMMUNOL JI Cell. Immunol. PD AUG 25 PY 1997 VL 180 IS 1 BP 10 EP 19 DI 10.1006/cimm.1997.1155 PG 10 WC Cell Biology; Immunology SC Cell Biology; Immunology GA XZ246 UT WOS:A1997XZ24600002 PM 9316634 ER PT J AU Tourian, K Alterman, A Metzger, D Rutherford, M Cacciola, JS McKay, JR AF Tourian, K Alterman, A Metzger, D Rutherford, M Cacciola, JS McKay, JR TI Validity of three measures of antisociality in predicting HIV risk behaviors in methadone-maintenance patients SO DRUG AND ALCOHOL DEPENDENCE LA English DT Article DE substance abuse; antisocial personality disorder; sociopathy; IDU; HIV risk factors ID INTRAVENOUS DRUG-ABUSERS; ADDICTION SEVERITY INDEX; PERSONALITY-DISORDER; PSYCHIATRIC-DIAGNOSIS; OPIATE ADDICTS; USERS AB Most opiate users are injection drug users (IDUs). A significant percentage of IDUs have antisocial personality disorder (APD). APD has been found by some researchers to be an additional risk factor for human immunodeficiency virus (HIV) infection in IDUs. The present study evaluated the association of sociodemographic characteristics, substance abuse history, and several measures of antisociality including the DSM-III-R diagnosis made by the Personality Disorder Examination, the California Psychological Inventory-Socialization Scale, and Hare's Revised Psychopathy Checklist, to behaviors associated with HIV risk in 289 opiate-dependent methadone-maintained subjects. The presence of drug-and sex-related risky behaviors measured by the Risk Assessment Battery was predicted more consistently by measures of personality traits associated with antisociality than by a diagnosis of APD. (C) 1997 Elsevier Science Ireland Ltd. C1 VET AFFAIRS MED CTR,PHILADELPHIA,PA. RP Tourian, K (reprint author), UNIV PENN,TREATMENT RES CTR,3900 CHESTNUT ST,PHILADELPHIA,PA 19104, USA. RI Metzger, David/D-9499-2012 FU NIDA NIH HHS [DA00172, DA05858, DA05186] NR 28 TC 20 Z9 20 U1 1 U2 3 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0376-8716 J9 DRUG ALCOHOL DEPEN JI Drug Alcohol Depend. PD AUG 25 PY 1997 VL 47 IS 2 BP 99 EP 107 DI 10.1016/S0376-8716(97)00076-8 PG 9 WC Substance Abuse; Psychiatry SC Substance Abuse; Psychiatry GA XR867 UT WOS:A1997XR86700003 PM 9298331 ER PT J AU DeNino, LA AF DeNino, LA TI Duty calls SO HOSPITALS & HEALTH NETWORKS LA English DT Letter C1 S TEXAS VET HLTH CARE SYST,SAN ANTONIO,TX. RP DeNino, LA (reprint author), UNIV TEXAS,HLTH SCI CTR,7703 FLOYD CURL DR,SAN ANTONIO,TX 78285, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU AMER HOSPITAL PUBL INC PI CHICAGO PA 737 N MICHIGAN AVE, CHICAGO, IL 60611-2615 SN 1068-8838 J9 HOSP HEALTH NETWORK JI Hosp. Health Netw. PD AUG 20 PY 1997 VL 71 IS 16 BP 10 EP 10 PG 1 WC Health Policy & Services SC Health Care Sciences & Services GA XR946 UT WOS:A1997XR94600002 ER PT J AU Kiefe, CI Williams, OD Bild, DE Lewis, CE Hilner, JE Oberman, A AF Kiefe, CI Williams, OD Bild, DE Lewis, CE Hilner, JE Oberman, A TI Regional disparities in the incidence of elevated blood pressure among young adults - The CARDIA study SO CIRCULATION LA English DT Article DE blood pressure; epidemiology; risk factors; hypertension; prevention ID 15-YEAR FOLLOW-UP; UNITED-STATES; POPULATION; MORTALITY; AGE; HYPERTENSION; HEALTH; WOMEN; MEN AB Background Within the United States, little is known about regional disparities in blood pressure (BP), their changes over time. or explanations for their existence. Methods and Results A population-based cohort of 5115 black and white men and women, 18 to 30 years old in 1985-1986 (balanced on age, race, sex, and education), was followed up for 7 years in four centers: Birmingham, Ala; Chicago, III; Minneapolis, Minn; and Oakland, Calif. Differences in elevated BP (EBP) prevalence among centers at years 0, 2, 5, and 7 and in 7-year incidence of EBP were assessed. Sociodemographic and dietary variables, physical activity, weight, smoking and alcohol were considered. Ar year 0, no regional differences were seen. Seven years later, there was marked variability in prevalence of EBP overall and fur both black and white men, from a low in Chicago (9% for black men and 5% for white men) to a high in Birmingham (25% for black men and 14% for white men). Birmingham also had the highest 7-year incidence (11%) and overall prevalence at year 7 (14%). The adjusted odds ratios, with Birmingham as referent (95% CIs), for 7-year incidence of EBP overall were 0.38 (0.24, 0.60) for Chicago, 0.37 (0.24, 0.57) for Minneapolis, and 0.74 (0.52, 1.07) for Oakland. Conclusions Regional disparities are absent at baseline but become apparent as the cohort ages. These differences are not fully explained by the available behavioral and sociodemographic characteristics. C1 BIRMINGHAM VA MED CTR,BIRMINGHAM,AL. NHLBI,NIH,BETHESDA,MD 20892. RP Kiefe, CI (reprint author), UNIV ALABAMA,DIV PREVENT MED,1717 11TH AVE S,MT 700,BIRMINGHAM,AL 35205, USA. FU NHLBI NIH HHS [N01-HC-48048, N01-HC-48047, N01-HC-48049] NR 35 TC 22 Z9 23 U1 0 U2 1 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD AUG 19 PY 1997 VL 96 IS 4 BP 1082 EP 1088 PG 7 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA XR082 UT WOS:A1997XR08200008 PM 9286933 ER PT J AU Street, VA Tempel, BL AF Street, VA Tempel, BL TI Physical mapping of potassium channel gene clusters on mouse chromosomes three and six SO GENOMICS LA English DT Article ID CPG ISLANDS; YEAST; TRANSFORMATION; CLONES; RECOMBINATION; REGIONS; BRAIN; DNA; SEQUENCES; HOMOLOGY AB Mammalian voltage-gated K channel genes have been divided into four subfamilies (Shaker, Shab, Shal, and Shaw) based on their sequence identity and similarity to related genes in Drosophila. Genetic mapping of the voltage-gated K channel genes has shown that similar multigene clusters exist on mouse Chr 3 and 6 and suggests that the clusters may have arisen through chromosomal duplication. In this report, YAC-based physical maps of the clustered mouse Shaker-like K channel genes have been constructed using restriction endonuclease and yeast chromosome fragmentation approaches. These data define the physical spacing as 5'-Kcna3-(60 kb)-Kcna2-(90 kb)-Kcna8-3' on Chr 3, and as 5'-Kcna6-(80 kb)-Kcna1-(110 kb)-Kcna5-3' on Chr 6, with all genes oriented in a head-to-tail manner within their respective clusters. These detailed physical maps of both K channel gene clusters provide additional support for the idea of an ancient genome tetraploidization event. (C) 1997 Academic Press. C1 UNIV WASHINGTON, SCH MED, VM BLOEDEL HEARING RES CTR, DEPT OTOLARYNGOL HEAD & NECK SURG, SEATTLE, WA 98195 USA. UNIV WASHINGTON, SCH MED, DEPT PHARMACOL, SEATTLE, WA 98195 USA. VET AFFAIRS PUGET SOUND HLTH CARE SYST, GERIATR RES EDUC & CLIN CTR 182B, SEATTLE, WA 98108 USA. FU NINDS NIH HHS [R01-NS27206] NR 41 TC 5 Z9 5 U1 0 U2 1 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0888-7543 EI 1089-8646 J9 GENOMICS JI Genomics PD AUG 15 PY 1997 VL 44 IS 1 BP 110 EP 117 DI 10.1006/geno.1997.4799 PG 8 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA XT456 UT WOS:A1997XT45600013 PM 9286706 ER PT J AU Alkhatib, G Ahuja, SS Light, D Mummidi, S Berger, EA Ahuja, SK AF Alkhatib, G Ahuja, SS Light, D Mummidi, S Berger, EA Ahuja, SK TI CC chemokine receptor 5-mediated signaling and HIV-1 co-receptor activity share common structural determinants - Critical residues in the third extracellular loop support HIV-1 fusion SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID RECOMBINANT VACCINIA VIRUS; ENVELOPE GLYCOPROTEIN; EXPRESSION; CELLS AB There is a close correspondence between the ability of RANTES and macrophage inflammatory proteins 1 alpha and 1 beta to activate CC chemokine receptor 5 (CCR5) and the ability to inhibit CCR5-dependent membrane fusion mediated by the envelope glycoprotein of human immuno-deficiency virus (HIV), type 1, This finding suggests that some of the structural determinants for CC chemokine/CCR5 interactions and CCR5 HIV-1 fusion co-receptor activity may be shared, Recent studies rising human CCR5/CCR2B chimeras have suggested that the determinants of CCR5 co-receptor activity are complex and may involve multiple extracellular receptor domains and that viral co-receptor activity is dissociable from ligand-dependent signaling responses, However, conclusive evidence demonstrating an important role for the second and third extracellular regions of human CCR5 is lacking, Furthermore, to determine whether the determinants for CCR5 co-receptor activity overlap with those required for agonist activity, studies that compare the chemokine specificity for inhibition of envelope-mediated cell fusion and the agonist profile of chimeric receptors are necessary, In the present report, using a series of CCR5/CCR2B chimeras we ascribe an important role for the second and third extracellular loop of CCR5 in supporting the co-receptor activity of CCR5, We also provide evidence that the intracytoplasmic tail of CCR5 does not play an important role in supporting HIV-1 entry, The hypothesis that the structural determinants for CC chemokine/CCR5 interactions and CCR5 HIV-1 fusion co-receptor activity may be shared was confirmed by two novel observations: first, the fusion activity supported by two hybrid receptors could be inhibited by both RANTES and monocyte chemoattractant protein-1, chemokines specific to CCR5 and CCR2B, respectively; and second, the chemokine specificity for inhibition of envelope-mediated cell fusion matched the agonist profile of these hybrid receptors. These data shed new light on the structural determinants involved in these distinct activities of CCR5 and may have important implications for the development of CCR5-targeted anti-viral compounds. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. NIAID,VIRAL DIS LAB,NIH,BETHESDA,MD 20892. S TEXAS VET HLTH CARE SYST,SAN ANTONIO,TX 78284. RI Mummidi, Srinivas/C-1004-2008 OI Mummidi, Srinivas/0000-0002-4068-6380 NR 30 TC 74 Z9 74 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD AUG 8 PY 1997 VL 272 IS 32 BP 19771 EP 19776 DI 10.1074/jbc.272.32.19771 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA XQ059 UT WOS:A1997XQ05900023 PM 9242636 ER PT J AU Schlesinger, N AF Schlesinger, N TI Identification of Gulf War syndrome: Methodological issues and medical illnesses SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Letter RP Schlesinger, N (reprint author), VET AFFAIRS MED CTR,UNIV & WOODLAND AVE,PHILADELPHIA,PA 19104, USA. NR 3 TC 1 Z9 1 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD AUG 6 PY 1997 VL 278 IS 5 BP 383 EP 383 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA XN518 UT WOS:A1997XN51800016 PM 9244322 ER PT J AU Gilkeson, GS Mudgett, JS Seldin, MF Ruiz, P Alexander, AA Misukonis, MA Pisetsky, DS Weinberg, JB AF Gilkeson, GS Mudgett, JS Seldin, MF Ruiz, P Alexander, AA Misukonis, MA Pisetsky, DS Weinberg, JB TI Clinical and serologic manifestations of autoimmune disease in MRL-lpr/lpr mice lacking nitric oxide synthase type 2 SO JOURNAL OF EXPERIMENTAL MEDICINE LA English DT Article ID MONOMETHYL-L-ARGININE; ANTIMICROBIAL ACTIVITY; LPR MICE; EXPRESSION; CELLS; GENE; GLOMERULONEPHRITIS; VASCULITIS; MECHANISMS; ACTIVATION AB Nitric oxide (NO) is an important mediator of the inflammatory response. MRL-lpr/lpr mice overexpress inducible nitric oxide synthase (NOS2) and overproduce NO in parallel with the development of an autoimmune syndrome with a variety of inflammatory manifestations. In previous studies, we showed that inhibiting NO production with the nonselective nitric oxide synthase (NOS) inhibitor NG-monomethyl-arginine reduced glomerulonephritis, arthritis, and vasculitis in MRL-lpr/lpr mice. To define further the role of NO and NOS2 in disease in MRL-lpr/lpr mice, mice with targeted disruption of NOS2 were produced by homologous recombination and bred to MRL-lpr/lpr mice to the N4 generation. MRL-lpr/lpr littermates homozygous for disrupted NOS2 (-/-), heterozygous for disrupted NOS2 (+/-), or wildtype (+/+) were derived for this study. Measures of NO production were markedly decreased in the MRL-lpr/lpr (-/-) mice compared with MRL-lpr/lpr (+/+) mice, with intermediate production by the MRL-lyr/lpr (+/-) mice. There was no detectable NOS2 protein by immunoblot analysis of the spleen, liver, kidney, and peritoneal macrophages of the (-/-) animals, whereas that of (+/+) was high and (+/-) intermediate. The (-/-) mice developed glomerular and synovial pathology similar to that of the (+/-) and (+/+) mice. However, (-/-) mice and (+/-) mice had significantly less vasculitis of medium-sized renal vessels than (+/+) mice. IgG rheumatoid factor levels were significantly lower in the (-/-) mice as compared with (+/+) mice, but levels of anti-DNA antibodies were comparable in all groups. Our findings show that NO derived from NOS2 has a variable impact on disease manifestations in MRL-lpr/lpr mice, suggesting heterogeneity in disease mechanisms. C1 VET AFFAIRS MED CTR,DURHAM,NC 27705. RALPH H JOHNSON VET AFFAIRS MED CTR,CHARLESTON,SC 29425. MED UNIV S CAROLINA,CHARLESTON,SC 29425. MERCK RES LABS,RAHWAY,NJ 07065. UNIV CALIF DAVIS,DAVIS,CA 95616. UNIV MIAMI,MED CTR,MIAMI,FL 33136. DUKE UNIV,MED CTR,DURHAM,NC 27705. FU NIAMS NIH HHS [AR-39162] NR 37 TC 81 Z9 81 U1 0 U2 0 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 SN 0022-1007 J9 J EXP MED JI J. Exp. Med. PD AUG 4 PY 1997 VL 186 IS 3 BP 365 EP 373 DI 10.1084/jem.186.3.365 PG 9 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA XP998 UT WOS:A1997XP99800004 PM 9236188 ER PT J AU Gallik, DM Kim, SG Ferrick, KJ Roth, JA Fisher, JD AF Gallik, DM Kim, SG Ferrick, KJ Roth, JA Fisher, JD TI Efficacy and safety of sotalol in patients with refractory atrial fibrillation or flatter SO AMERICAN HEART JOURNAL LA English DT Article ID SINUS RHYTHM; CATHETER ABLATION; DRUG-TREATMENT; ORAL SOTALOL; FLUTTER; ARRHYTHMIAS; MAINTENANCE AB Sotalol's usefulness in treatment of atrial fibrillation and atrial flutter is unproven. This study evaluated (1) the efficacy of sotalol in preventing recurrences of paroxysmal atrial fibrillation or atrial flutter and controlling ventricular rate (in chronic atrial fibrillation or relapse of paroxysmal atrial arrhythmias), (2) the safety of sotalol, and (3) predictors of sotalol efficacy. Thirty-three patients, 28 with paroxysmal and five with chronic atrial fibrillation or atrial flutter, received an average dose of 265 +/- 119 mg of oral sotalol per day. During a 10 +/- 12 month Follow-up, recurrence rate for paroxysmal arrhythmia was 64%, with a 50% recurrence at 4.6 months. For patients with chronic atrial fibrillation, ventricular rates were well controlled with sotalol administration (136 +/- 33 beats/min versus 88 +/- 23 beats/min; p = 0.04). No patient with chronic atrial fibrillation converted to sinus rhythm during the study. Side effects necessitated sotalol discontinuation in three patients. By multivariate analysis, younger age, higher election fraction, and absence of hypertension independently predicted sotalol efficacy. C1 MONTEFIORE MED CTR,NEW YORK,NY. RP Gallik, DM (reprint author), UNIV CALIF LOS ANGELES,SCH MED,W LOS ANGELES VET AFFAIRS MED CTR,DIV CARDIOL,LOS ANGELES,CA 90073, USA. NR 23 TC 8 Z9 8 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0002-8703 J9 AM HEART J JI Am. Heart J. PD AUG PY 1997 VL 134 IS 2 BP 155 EP 160 DI 10.1016/S0002-8703(97)70118-2 PN 1 PG 6 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA XX296 UT WOS:A1997XX29600001 PM 9313591 ER PT J AU Lieberman, DA Oehlke, M Helfand, M AF Lieberman, DA Oehlke, M Helfand, M TI Risk factors for Barrett's esophagus in community-based practice SO AMERICAN JOURNAL OF GASTROENTEROLOGY LA English DT Article ID PREVALENCE AB Objectives: Endoscopy is often performed in patients with gastroesophageal reflux (GER) disease because of concern about the presence of Barrett's esophagus (BE). The purpose of this study was to determine,whether the duration of GER symptoms and/or a history of esophagitis was associated with an increased risk of BE. Methods: This was an observational, prospective, community-based study. Consecutive patients undergoing elective endoscopy because of GER symptoms were enrolled. Endoscopy reports and pathological findings were reviewed to classify patients as having no esophagitis, esophagitis, or probable BE. Correlations with duration of symptoms and a history of esophagitis were analyzed. Results: In all, 701 of 2641 patients (27%) undergoing elective endoscopy had GER symptoms, and 77 of these patients had probable BE. Compared with patients with GER symptoms for less than 1 yr, the odds ratio for BE in patients with GER symptoms for 1-5 years was 3.0 and increased to 6.4 in patients with symptoms for more than 10 yr (p < 0.001). A history of esophagitis was not an independent risk factor for BE (p = 0.17). Conclusions: Prevalence of BE is strongly associated with duration of GER symptoms. C1 OREGON HLTH SCI UNIV, DEPT MED, DIV GEN MED, PORTLAND, OR 97207 USA. PORTLAND VET AFFAIRS MED CTR, GASTROENTEROL SECT, PORTLAND, OR 97207 USA. RP OREGON HLTH SCI UNIV, DEPT MED, DIV GASTROENTEROL, 111-A, POB 1034, PORTLAND, OR 97207 USA. NR 12 TC 197 Z9 200 U1 1 U2 3 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0002-9270 EI 1572-0241 J9 AM J GASTROENTEROL JI Am. J. Gastroenterol. PD AUG PY 1997 VL 92 IS 8 BP 1293 EP 1297 PG 5 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA XP756 UT WOS:A1997XP75600011 PM 9260792 ER PT J AU Strausbaugh, L AF Strausbaugh, L TI Antimicrobial resistance: Problems, laments, and hopes SO AMERICAN JOURNAL OF INFECTION CONTROL LA English DT Editorial Material ID STAPHYLOCOCCUS-AUREUS RP Strausbaugh, L (reprint author), PORTLAND VA MED CTR,648-111F,3710 SW VET HOSP RD,PORTLAND,OR 97207, USA. NR 15 TC 8 Z9 8 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0196-6553 J9 AM J INFECT CONTROL JI Am. J. Infect. Control PD AUG PY 1997 VL 25 IS 4 BP 294 EP 296 DI 10.1016/S0196-6553(97)90020-1 PG 3 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA XR045 UT WOS:A1997XR04500002 PM 9276540 ER PT J AU Dell'Italia, LJ Balcells, E Meng, QC Su, XF Schultz, D Bishop, SP Machida, N Straeter-Knowlen, IM Hankes, GH Dillon, R Cartee, RE Oparil, S AF Dell'Italia, LJ Balcells, E Meng, QC Su, XF Schultz, D Bishop, SP Machida, N Straeter-Knowlen, IM Hankes, GH Dillon, R Cartee, RE Oparil, S TI Volume-overload cardiac hypertrophy is unaffected by ACE inhibitor treatment in dogs SO AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY LA English DT Article DE angiotensin II; angiotensin-converting enzyme inhibitor; heart failure; congestive ID ANGIOTENSIN-CONVERTING ENZYME; MESSENGER-RNA; HEART-FAILURE; RECEPTOR SUBTYPE; FORMING PATHWAYS; II RECEPTOR; SYSTEM; RENIN; RATS; EXPRESSION AB We tested the hypothesis that angiotensin-converting enzyme (ACE) inhibitor therapy prevents volume-overload hypertrophy in dogs with chronic mitral regurgitation (MR). Seven adult mongrel dogs receiving ramipril (R; 10 mg orally, twice/day) for 4 mo were compared with 11 dogs receiving no R (N) for 4 mo after induction of MR. Cine-magnetic resonance imaging demonstrated that left ventricular (LV) mass increased in the R-MR dogs [80 +/- 4 (SE) to 108 +/- 7 g, P < 0.01] and in the N-MR dogs (92 +/- 7 to 112 +/- 8 g, P < 0.001). LV myocyte cell length was greater in the R-MR and N-MR dogs (203 +/- 6 and 177 +/- 10 mu m, respectively) than in normal (144 +/- 4 mu m, P < 0.05) dogs. There was significant loss of the collagen weave pattern by scanning electron microscopy in both R-MR and N-MR dogs. LV ACE and chymase activities were significantly elevated in R-MR and N-MR compared with normal dogs. LV angiotensin II (ANG II) levels in the R-MR dogs (28 +/- 12 pg/g) were reduced to levels seen in normal dogs (28 +/- 4 pg/g) compared with N-MR dogs (72 +/- 11 pg/g, P < 0.05). Steady-state AT(1)-receptor mRNA levels decreased 66% in N-MR compared with normal dogs (P < 0.001) and increased 1.5-fold in R-MR compared with normal dogs (P < 0.01). Thus upregulation of the AT(1) receptor in the R-MR hearts may provide a mechanism by which normal intracardiac ANG II levels could continue to mediate LV hypertrophy. However, the mechanism of dissolution collagen weave in both N-MR and R-MR hearts may be related to the stretch of volume overload. C1 Univ Alabama, Birmingham Vet Affairs Med Ctr, Div Cardiovasc Dis, Birmingham, AL 35294 USA. Auburn Univ, Coll Vet Med, Auburn, AL 36849 USA. RP Dell'Italia, LJ (reprint author), Univ Alabama, Dept Med, Div Cardiol, 310 Lyons Harrison Res Bldg,701 S 19th St, Birmingham, AL 35294 USA. RI Machida, Noboru/H-2729-2013 OI Machida, Noboru/0000-0003-2960-1523 FU NHLBI NIH HHS [2T32HL-07457-16A1, HL-17667, R01-HL-54816] NR 34 TC 80 Z9 82 U1 0 U2 3 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0363-6135 J9 AM J PHYSIOL-HEART C JI Am. J. Physiol.-Heart Circul. Physiol. PD AUG PY 1997 VL 273 IS 2 BP H961 EP H970 PG 10 WC Cardiac & Cardiovascular Systems; Physiology; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Physiology GA XQ295 UT WOS:A1997XQ29500053 PM 9277516 ER PT J AU Deneke, SM Harford, PH Lee, KY Deneke, CF Wright, SE Jenkinson, SG AF Deneke, SM Harford, PH Lee, KY Deneke, CF Wright, SE Jenkinson, SG TI Induction of cystine transport and other stress proteins by disulfiram: Effects on glutathione levels in cultured cells SO AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY LA English DT Article ID ENDOTHELIAL-CELLS; SODIUM ARSENITE; DIETHYL MALEATE; SUPEROXIDE-DISMUTASE; LIPID-PEROXIDATION; HYPERBARIC-OXYGEN; PROTECTIVE AGENTS; MAMMALIAN-CELLS; ACID UPTAKE; RAT AB Disulfiram (Antabuse) (DSF) has been reported to protect rats and other animals from the effects of hyperbaric hyperoxia at 4 to 6 ATA (atmospheres). In contrast, DSF and diethyldithiocarbamate (DDC), its metabolite, accelerate the toxic effects in rats of 100% oxygen at 1 to 2 ATA. We have examined the effects of DSF and DDC on glutathione (GSH) levels in bovine pulmonary artery endothelial cells and Chinese hamster ovary cells. Increases in intracellular GSH occurred 8 to 24 h after addition of DSF to the culture media. These increases in intracellular GSH were associated with increases in the rate of uptake of cystine into the cells. DDC was a less effective inducer of cystine uptake and increased intracellular GSH levels than was DSF. At the concentrations used, neither DDC nor DSF caused significant decreases intracellular superoxide dismutase levels. Exogenous sulfhydryl compounds including GSH and cysteine partially blocked the induction of cystine transport by DSF or DDC, suggesting that the induction might be mediated through a sulfhydryl reaction between DSF and some cellular components. The increases in GSH in the cultured cells were not significant by 4 h of exposure, In contrast, other stress proteins including heme oxygenase are induced by 2 to 4 h after DSF addition, In previously reported in vitro studies, DSF treatment protected against hyperbaric oxygen damage after as little as 1 to 4 h pre-exposure. This suggests that effects of DSF exposure other than GSH augmentation may be responsible for the protective effects seen in vivo. C1 AUDIE L MURPHY MEM VET ADM MED CTR,S TEXAS VET HLTH CARE SYST,SAN ANTONIO,TX 78284. RP Deneke, SM (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV PULM DIS CRIT CARE MED,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. FU NHLBI NIH HHS [HL-32824] NR 47 TC 6 Z9 6 U1 1 U2 2 PU AMER LUNG ASSOC PI NEW YORK PA 1740 BROADWAY, NEW YORK, NY 10019 SN 1044-1549 J9 AM J RESP CELL MOL JI Am. J. Respir. Cell Mol. Biol. PD AUG PY 1997 VL 17 IS 2 BP 227 EP 234 PG 8 WC Biochemistry & Molecular Biology; Cell Biology; Respiratory System SC Biochemistry & Molecular Biology; Cell Biology; Respiratory System GA XR280 UT WOS:A1997XR28000013 PM 9271311 ER PT J AU Ofman, JJ Soll, AH Kahn, KL AF Ofman, JJ Soll, AH Kahn, KL TI Management of Helicobacter pylori - Positive patients with dyspepsia - Response SO ANNALS OF INTERNAL MEDICINE LA English DT Letter ID INFECTION; TESTS C1 UNIV CALIF LOS ANGELES,LOS ANGELES,CA 90024. RP Ofman, JJ (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 5 TC 0 Z9 0 U1 0 U2 0 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD AUG 1 PY 1997 VL 127 IS 3 BP 244 EP 244 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA XN129 UT WOS:A1997XN12900019 ER PT J AU Graybill, JR Najvar, LK Luther, MF Fothergill, AW AF Graybill, JR Najvar, LK Luther, MF Fothergill, AW TI Treatment of murine disseminated candidiasis with L-743,872 SO ANTIMICROBIAL AGENTS AND CHEMOTHERAPY LA English DT Article ID IN-VIVO RESPONSE; AMPHOTERICIN-B; FLUCONAZOLE TREATMENT; CILOFUNGIN LY121019; ALBICANS INFECTION; RESISTANT CANDIDA; ANTIFUNGAL AGENT; CELL-WALL; SUSCEPTIBILITY; AIDS AB L-743,872 (M991), which is a pneumocandin derivative, was evaluated in a mouse model of disseminated candidiasis caused by a fluconazole-resistant isolate of Candida albicans, In immunocompetent mice M991 prolonged survival at doses as low as 0.0125 mg/kg of body weight per day. In neutropenic mice 0.05 mg/kg was the lowest effective dose. M991 is a very potent drug for treatment of disseminated candidiasis. C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. RP Graybill, JR (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,INFECT DIS SECT 111F,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. NR 21 TC 59 Z9 60 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0066-4804 J9 ANTIMICROB AGENTS CH JI Antimicrob. Agents Chemother. PD AUG PY 1997 VL 41 IS 8 BP 1775 EP 1777 PG 3 WC Microbiology; Pharmacology & Pharmacy SC Microbiology; Pharmacology & Pharmacy GA XP605 UT WOS:A1997XP60500028 PM 9257759 ER PT J AU McGinnis, MR Pasarell, L Sutton, DA Fothergill, AW Cooper, CR Rinaldi, MG AF McGinnis, MR Pasarell, L Sutton, DA Fothergill, AW Cooper, CR Rinaldi, MG TI In vitro evaluation of voriconazole against some clinically important fungi SO ANTIMICROBIAL AGENTS AND CHEMOTHERAPY LA English DT Article AB Voriconazole was compared to amphotericin B. fluconazole, and itraconazole by using an in vitro macrobroth dilution test based upon current National Committee for clinical Laboratory Standards tentative standards against the dimorphic fungi and several opportunistic molds and yeasts, In all instances, the voriconazole MICs were lower than those of fluconazole. In most instances, tbe MICs were lower than the recorded MICs of amphotericin 15 and itraconazole. C1 UNIV TEXAS,MED BRANCH,WHO,COLLABORATING CTR TROP DIS,GALVESTON,TX 77555. UNIV TEXAS,HLTH SCI CTR,FUNGUS TESTING LAB,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,CLIN MICROBIOL LABS,SAN ANTONIO,TX 78284. RP McGinnis, MR (reprint author), UNIV TEXAS,MED BRANCH,DEPT PATHOL,301 UNIV BLVD,GALVESTON,TX 77555, USA. NR 21 TC 85 Z9 86 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0066-4804 J9 ANTIMICROB AGENTS CH JI Antimicrob. Agents Chemother. PD AUG PY 1997 VL 41 IS 8 BP 1832 EP 1834 PG 3 WC Microbiology; Pharmacology & Pharmacy SC Microbiology; Pharmacology & Pharmacy GA XP605 UT WOS:A1997XP60500042 PM 9257773 ER PT J AU Volpicelli, JR Rhines, KC Rhines, JS Volpicelli, LA Alterman, AI OBrien, CP AF Volpicelli, JR Rhines, KC Rhines, JS Volpicelli, LA Alterman, AI OBrien, CP TI Naltrexone and alcohol dependence - Role of subject compliance SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Article ID ETHANOL-CONSUMPTION; STRATEGIES AB Background: Two previous double-blind, placebo-controlled studies demonstrated that naltrexone (50 mg/d) reduces alcohol drinking in alcohol-dependent subjects. In both studies, treatment compliance was excellent. However, a robust treatment effect size for naltrexone relative to placebo has been shown for compliant subjects but not for subjects who missed research visits. The goal of this study was to determine the effectiveness of naltrexone in subjects who received psychosocial treatment in a more naturalistic setting with respect to the role of treatment attendance and medication compliance. Methods: Ninety-seven alcohol-dependent subjects were randomly assigned to receive either naltrexone (n=48) or matching placebo (n=49) for 12 weeks. All subjects received individual counseling (twice per week for the first month followed by once per week). Results: Overall, naltrexone showed only modest effects in reducing alcohol drinking for the 12 weeks of treatment. However, naltrexone treatment efficacy improved across a variety of outcome measures for subjects who completed treatment and were highly compliant with taking medication. Conclusions: Naltrexone is clinically effective relative to placebo in individuals who comply with the treatment protocol and take medication. The modest treatment effects in the entire sample suggest that the clinical efficacy of naltrexone could be improved by enhancing treatment compliance. C1 UNIV PENN, DEPT PSYCHIAT, PHILADELPHIA, PA 19104 USA. VET AFFAIRS MED CTR, PHILADELPHIA, PA USA. RUTGERS STATE UNIV, DEPT PSYCHOL, PISCATAWAY, NJ USA. FU NIAAA NIH HHS [AA-07517]; NIDA NIH HHS [DA-06107] NR 26 TC 297 Z9 310 U1 1 U2 6 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60654-0946 USA SN 0003-990X EI 1538-3636 J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD AUG PY 1997 VL 54 IS 8 BP 737 EP 742 PG 6 WC Psychiatry SC Psychiatry GA XQ037 UT WOS:A1997XQ03700008 PM 9283509 ER PT J AU Sanders, VJ Felisan, SL Waddell, AE Conrad, AJ Schmid, P Kaufman, M Walsh, GO DeSalles, AAF Tourtellotte, WW AF Sanders, VJ Felisan, SL Waddell, AE Conrad, AJ Schmid, P Kaufman, M Walsh, GO DeSalles, AAF Tourtellotte, WW TI Presence of herpes simplex DNA in surgical tissue from human epileptic seizure foci detected by polymerase chain reaction - Preliminary study SO ARCHIVES OF NEUROLOGY LA English DT Article ID TEMPORAL-LOBE EPILEPSY; VIRUS; BRAIN; ENCEPHALITIS; PATHOLOGY; TYPE-1 AB Objectives: To determine whether herpes simplex virus causes monofocal epilepsy and to assess the presence of herpes simplex virus 1 (HSV-1) and HSV-2 in surgical specimens from patients with epilepsy by using polymerase chain reaction and Southern blot analysis. Background: Herpes simplex virus is a common neurotropic virus capable of latency within the central nervous system; it has a predilection for the temporal lobe. Central nervous system infection with HSV has been associated with seizure activity. Design and Methods: Surgical specimens were removed from 50 patients as part of a treatment protocol for monofocal epilepsy. Neuropathological classification was done, and adjacent sections were screened for HSV by using polymerase chain reaction. Tissues obtained post mortem from the temporal lobe cortex of persons with Alzheimer disease (n = 17), Parkinson disease (n = 14), or nonneurological disease (n = 17) served as controls. Results: Twenty (40%) of the 50 epilepsy cases and 2 (4%) of the 48 control cases had at least one sample that tested positive for HSV (P < .001). Sixty-seven percent (8/12) of the epilepsy cases with heterotopia were positive for HSV. Conclusions: There was a statistically significant difference in the frequency of HSV-positive surgical specimens from monofocal seizure epicenters compared with nonepilepsy control specimens. These data suggest an association of the virus with seizure activity. All specimens positive for HSV (surgical specimens and control specimens) should be examined to determine the activity or latency state of the virus and cellular localization. C1 W LOS ANGELES VET AFFAIRS MED CTR,NEUROL SERV 127A,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,RES SERV,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,DEPT SURG,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,INST BRAIN RES,LOS ANGELES,CA 90024. CALIF COMPREHENS EPILEPSY PROGRAM,LOS ANGELES,CA. FU NINDS NIH HHS [NS21908B] NR 21 TC 24 Z9 25 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-9942 J9 ARCH NEUROL-CHICAGO JI Arch. Neurol. PD AUG PY 1997 VL 54 IS 8 BP 954 EP 960 PG 7 WC Clinical Neurology SC Neurosciences & Neurology GA XQ778 UT WOS:A1997XQ77800005 PM 9267969 ER PT J AU Bailet, JW Lichtenstein, A Chen, G Mickel, RA AF Bailet, JW Lichtenstein, A Chen, G Mickel, RA TI Inhibition of lymphocyte function by head and neck carcinoma cell line soluble factors SO ARCHIVES OF OTOLARYNGOLOGY-HEAD & NECK SURGERY LA English DT Article; Proceedings Paper CT 35th Annual Meeting of the American-Society-for-Head-and-Neck-Surgery CY APR 21-22, 1993 CL LOS ANGELES, CA SP Amer Soc Head & Neck Surg ID ACTIVATED KILLER-CELLS; CANCER-PATIENTS; RECOMBINANT INTERLEUKIN-2; ADOPTIVE IMMUNOTHERAPY; CYTO-TOXICITY; LAK CELLS; SUPPRESSION; NODES; LYSIS; METASTASES AB Background: Immunosuppression in patients with head and neck cancer is well recognized. Previous investigations have demonstrated graded immunosuppression that becomes more pronounced as lymphocyte activity is measured closer to the primary neoplasm. In fresh tumors, a soluble factor has been identified that may partly account for the observed graded immunosuppression. Objective: To examine the effect of soluble factors produced by head and neck sqamous cell carcinoma cell lines on the generation of lymphokine activated killer cell cytotoxicity and peripheral blood lymphocyte proliferation induced by interleukin 2, concanavalin A, and phytohemagglutinin. Design: Conditioned supernatant fluids were generated in a 4-day incubation period, using 5 head and neck squamous cell carcinoma cell lines, and were assayed for the ability to inhibit the generation of lymphokine activated killer cell cytotoxicity and naive peripheral blood lymphocyte proliferation induced by interleukin 2, concanavalin A, and phytohemagglutinin. Results: All conditioned supernatant fluids significantly inhibited the generation of lymphokine activated killer cell cytotoxicity relative to controls, and this inhibition was dose dependent. In contrast, supernatant fluids from a myelogenous leukemic tumor cell line, K562, and an ovarian epithelial cell line, SKOV-3, failed to inhibit cytotoxicity. Supernatant fluids conditioned with head and neck squamous cell carcinoma cell lines also profoundly inhibited the naive peripheral blood lymphocyte proliferation induced by interleukin 2, concanavalin A, and phytohemagglutinin. Conclusions: These studies demonstrate that the cell lines of head and neck squamous cell carcinoma produce soluble factors that inhibit lymphocyte function. Furthermore, these experiments suggest that the inhibition previously observed with enzymatically disaggregated fresh tumors is due to factors produced by the tumor cells rather than by other cells within the tumor matrix. C1 UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,DEPT MED,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,DIV HEAD & NECK SURG,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA 90024. JONSSON COMPREHENS CANC CTR,LOS ANGELES,CA 90034. W LOS ANGELES VET AFFAIRS MED CTR,CTR CANC,LOS ANGELES,CA 90073. NR 31 TC 4 Z9 4 U1 0 U2 1 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0886-4470 J9 ARCH OTOLARYNGOL JI Arch. Otolaryngol. Head Neck Surg. PD AUG PY 1997 VL 123 IS 8 BP 855 EP 862 PG 8 WC Otorhinolaryngology; Surgery SC Otorhinolaryngology; Surgery GA XQ525 UT WOS:A1997XQ52500011 PM 9260552 ER PT J AU Hardman, WE Cameron, IL Beer, WH Speeg, KV Kadakia, SC Lang, KA AF Hardman, WE Cameron, IL Beer, WH Speeg, KV Kadakia, SC Lang, KA TI Transforming growth factor alpha distribution in rectal crypts as a biomarker of decreased colon cancer risk in patients consuming cellulose SO CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION LA English DT Article ID LOSE PROLIFERATIVE RESPONSE; CELL-PROLIFERATION; DIETARY CELLULOSE; TGF-ALPHA; CARCINOGENESIS; RATS; DIFFERENTIATE; NEOPLASIA; RECEPTOR; CALCIUM AB Data from rat experimental carcinogenesis studies indicate that supplemental dietary cellulose reduces the incidence of colon cancer. Epidemiology studies also indicate that high dietary fiber reduces the risk of colorectal cancer in humans. Patients diagnosed with sporadic adenomas were entered into a randomized clinical trial to determine if supplemental dietary cellulose would reduce the patients' risk for colon cancer. Immunohistochemical staining for transforming growth factor alpha (TGF-alpha) was done on biopsies of rectal mucosa taken from patients at the time of initial polypectomy and 1 year later. Results were evaluated for utility as a surrogate end point biomarker for reduction in colon cancer risk. There was a significant decrease in the fraction of the rectal crypt cells that stained for TGF-alpha in six of seven of the patients given the cellulose supplements but in only one of six of the patients not given cellulose. Thus, whether evaluated as a group or in individual patients, there was a significant decrease in TGF-alpha in rectal crypts due to cellulose intervention, which correlated with the expected ability of supplemental dietary cellulose to decrease the risk for colon cancer. Long-term testing of the ability of dietary cellulose to reduce adenoma recurrence is under way to validate the use of TGF-alpha as a surrogate end point biomarker. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. AUDIE MURPHY DIV,S TEXAS VET HLTH CARE SYST,SAN ANTONIO,TX 78284. BROOKE ARMY MED CTR,SAN ANTONIO,TX 78234. WILFORD HALL USAF MED CTR,SAN ANTONIO,TX 78236. RP Hardman, WE (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT CELLULAR & STRUCT BIOL,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. NR 38 TC 10 Z9 10 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 1055-9965 J9 CANCER EPIDEM BIOMAR JI Cancer Epidemiol. Biomarkers Prev. PD AUG PY 1997 VL 6 IS 8 BP 633 EP 637 PG 5 WC Oncology; Public, Environmental & Occupational Health SC Oncology; Public, Environmental & Occupational Health GA XR010 UT WOS:A1997XR01000012 PM 9264277 ER PT J AU Uy, HL Mundy, GR Boyce, BF Story, BM Dunstan, CR Yin, JJ Roodman, GD Guise, TA AF Uy, HL Mundy, GR Boyce, BF Story, BM Dunstan, CR Yin, JJ Roodman, GD Guise, TA TI Tumor necrosis factor enhances parathyroid hormone-related protein-induced hypercalcemia and bone resorption without inhibiting bone formation in vivo SO CANCER RESEARCH LA English DT Article ID COLONY-STIMULATING FACTOR; SQUAMOUS-CELL CARCINOMA; HUMORAL HYPERCALCEMIA; IN-VIVO; NUDE-MICE; FORMATION INVITRO; TNF-ALPHA; MALIGNANCY; INTERLEUKIN-1-ALPHA; SERUM AB Humoral hypercalcemia of malignancy results from the effects of tumor-produced factors on bone, kidney, and intestine that disrupt normal calcium homeostasis, Although parathyroid hormone-related protein (PTHrP) is a major mediator of the syndrome, tumors also produce other hypercalcemic factors, such as tumor necrosis factor (TNF), which may modulate the effects of PTHrP, It has been postulated that TNF may counteract the stimulatory effects of PTHrP on bone formation, To examine the effects of TNF on PTHrP-induced changes in calcium and bone metabolism a murine tumor model of hypercalcemia was used. Nude mice were inoculated with Chinese hamster ovarian (CHO) cells expressing human TNF (CHO/TNF) or nontransfected CHO cells (CHO/-) and further treated with injections of human PTHrP(1-34) or vehicle, The effects of TNF, PTHrP, and the combination of the two factors on blood ionized calcium, osteoclast recruitment, and bone histomorphometry were evaluated. Mice bearing CHO/TNF tumors that were injected with PTHrP had significantly higher calcium concentrations, increased committed osteoclast progenitors, and mature osteoclasts as well as enhanced bone resorption compared with mice bearing CHO/TNF tumors injected with vehicle or those bearing CHO/- tumors injected with PTHrP or vehicle, A 2-fold increase in new woven bone formed in the calvaria at sites of previous bone resorption was observed in CHO/TNF mice treated with PTHrP, Bone formation rates in the vertebrae were similar in both CHO/- and CHO/TNF mice treated with PTHrP, These data demonstrate that the hypercalcemic effects of PTHrP are enhanced by TNF and that this effect is due to the increased production of committed osteoclast precursors with a subsequent increase in osteoclastic bone resorption, Furthermore, PTHrP caused a coupled increase in osteoclastic bone resorption and new bone formation that was not inhibited by TNF, These findings highlight the complex interactions that may occur between tumor-produced factors on bone that result in malignancy-associated hypercalcemia and suggest that TNF may not be responsible for the decreased bone formation seen in some patients with this condition. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV ENDOCRINOL,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. AMGEN INC,THOUSAND OAKS,CA 91320. RI Dunstan, Colin/G-6214-2013 OI Dunstan, Colin/0000-0001-7586-4071 FU NCI NIH HHS [CA 40035]; NIAMS NIH HHS [AR 01899] NR 36 TC 44 Z9 45 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD AUG 1 PY 1997 VL 57 IS 15 BP 3194 EP 3199 PG 6 WC Oncology SC Oncology GA XM812 UT WOS:A1997XM81200024 PM 9242449 ER PT J AU Levine, G Khan, MM Kleiman, NS Raizner, AE Jeroudi, MO Mickelson, J Ali, MN AF Levine, G Khan, MM Kleiman, NS Raizner, AE Jeroudi, MO Mickelson, J Ali, MN TI Use of bare-mounted Palmaz-Schatz stents employing the stent saddle technique on the delivery balloon: A single center experience SO CATHETERIZATION AND CARDIOVASCULAR DIAGNOSIS LA English DT Article DE Palmaz-Schatz stent; coronary angioplasty; abrupt vessel closure AB The major limitations of the Palmaz-Schatz stent stem from the design of its stent delivery system (SDS). The SDS is bulky and has poor trackability in lesions with proximal tortuosity and/or vessel calcification. The use of bare-mounted Palmaz-Schatz stents on low profile balloons represents an alternate approach for lesions that are not accessible for stenting with the SDS, Thus we evaluated the indications, procedural success rate, and in-hospital complications of patients undergoing bare stenting at a single center between 1 October 1995 through 30 September 1996. A total of 363 coronary interventions were performed during this period, including coronary stenting in 194 vessels. In 18 of these 194 vessels, bare-mounted Palmaz-Schatz stents were used. The indications for bare stenting were: inability to deliver the Palmaz-Schatz stent on SDS for suboptimal angioplasty results or acute/threatened abrupt closure; use of half stents; stenting in vessels < 3.0 mm; intermediate disease in the proximal segment that would have precluded optimal visualization of stent placement; and use of guides 7 French or smaller. Bare stenting was successful in 15 of the 18 patients (vessels) in whom it was attempted, There were no deaths, myocardial infarctions, stent thrombosis, repeat interventions, or significant bleeding in patients with successful bare stent delivery, The stents were successfully retrieved in the three patients in whom the stent could not be advanced into the target coronary segment, One of these patients had a propagated spiral dissection prior to attempts at bare stenting and required emergent bypass surgery. The remaining two patients with failed deployment had suboptimal angioplasty results but had an uncomplicated hospital course, Thus bare stenting represents an alternate percutaneous approach to tackle suboptimal procedural results and/or complications in patients who have failed stent deployment with the standard sheathed stent delivery system currently available in the United States. (C) 1997 Wiley-Liss, Inc. C1 BAYLOR COLL MED,VA MED CTR,CARDIOL SECT,DEPT MED,HOUSTON,TX 77030. METHODIST HOSP,HOUSTON,TX 77030. NR 12 TC 7 Z9 7 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0098-6569 J9 CATHETER CARDIO DIAG JI Catheter. Cardiovasc. Diagn. PD AUG PY 1997 VL 41 IS 4 BP 361 EP 368 PG 8 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA XN788 UT WOS:A1997XN78800001 PM 9258473 ER PT J AU Bradley, J Forero, N Pho, H Escobar, B Kasinath, BS Anzueto, A AF Bradley, J Forero, N Pho, H Escobar, B Kasinath, BS Anzueto, A TI Progressive somnolence leading to coma in a 68-year-old man SO CHEST LA English DT Article ID ACYCLOVIR; NEUROTOXICITY C1 AUDIE L MURPHY MEM VET HOSP DIV,S TEXAS VET HLTH CARE SYST,SAN ANTONIO,TX. UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DIV NEPHROL,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DIV PULM DIS CRIT CARE MED,SAN ANTONIO,TX 78284. RP Bradley, J (reprint author), BROOKE ARMY MED CTR,SAN ANTONIO,TX, USA. NR 5 TC 4 Z9 4 U1 0 U2 0 PU AMER COLL CHEST PHYSICIANS PI NORTHBROOK PA 3300 DUNDEE ROAD, NORTHBROOK, IL 60062-2348 SN 0012-3692 J9 CHEST JI Chest PD AUG PY 1997 VL 112 IS 2 BP 538 EP 540 DI 10.1378/chest.112.2.538 PG 3 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA XQ816 UT WOS:A1997XQ81600042 PM 9266897 ER PT J AU MacLellan, WR Schneider, MD AF MacLellan, WR Schneider, MD TI Death by design - Programmed cell death in cardiovascular biology and disease SO CIRCULATION RESEARCH LA English DT Review DE apoptosis; gene transfer; heart failure; ischemia ID WILD-TYPE P53; ENDONUCLEASE ACTIVATION; DILATED CARDIOMYOPATHY; MEDIATED APOPTOSIS; NECROSIS-FACTOR; HEART-FAILURE; MESSENGER-RNA; GENE CED-3; BCL-2; EXPRESSION AB Programmed cell death (apoptosis) is recognized, increasingly, as a contributing cause of cardiac myocyte loss with ischemia/reperfusion injury, myocardial infarction, and long-standing heart failure. Although the exact mechanisms initiating apoptosis in these in vivo settings remain unproven, insights into the molecular circuitry controlling apoptosis more widely suggest the potential to protect mammalian ventricular muscle from apoptosis through one or more of these pathways, by pharmacological means or, conceivably, gene transfer. C1 BAYLOR COLL MED,MOL CARDIOL UNIT,HOUSTON,TX 77030. BAYLOR COLL MED,DEPT MED,HOUSTON,TX 77030. BAYLOR COLL MED,DEPT CELL BIOL,HOUSTON,TX 77030. BAYLOR COLL MED,DEPT PHYSIOL & MOL BIOPHYS,HOUSTON,TX 77030. BAYLOR COLL MED,HOUSTON VET AFFAIRS MED CTR,HOUSTON,TX 77030. FU NHLBI NIH HHS [R01 HL-47567, P01 HL-49953, R01 HL-52555] NR 101 TC 283 Z9 293 U1 0 U2 3 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7330 J9 CIRC RES JI Circ.Res. PD AUG PY 1997 VL 81 IS 2 BP 137 EP 144 PG 8 WC Cardiac & Cardiovascular Systems; Hematology; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Hematology GA XN585 UT WOS:A1997XN58500001 PM 9242174 ER PT J AU Eisenberg, LS Dirks, DD Gornbein, JA AF Eisenberg, LS Dirks, DD Gornbein, JA TI Subjective judgments of speech clarity measured by paired comparisons and category rating SO EAR AND HEARING LA English DT Article ID NATIONAL-ACOUSTIC-LABORATORIES; SOUND QUALITY JUDGMENTS; HEARING-AID SELECTION; FREQUENCY RESPONSES; LISTENING CONDITIONS; INTELLIGIBILITY; RELIABILITY; SENSITIVITY; LOUDNESS; STIMULI AB Objective: The purpose of this study was to compare listeners' subjective judgments of speech clarity via paired comparisons and category rating using stimulus conditions that varied in the relative spacing between stimulus items, producing either a wide or narrow range of performance. Design: Subjective judgments of speech clarity were measured via paired comparisons and category rating in 12 normal-hearing (Experiment 1) and eight hearing-impaired adults (Experiment 2). Sentences processed by six band-pass filters that increased monotonically in Articulation Index (AI) estimates constituted the stimuli to be judged. Using subsets of three filters from the group of six, subjective judgments were additionally obtained for stimulus conditions in which the performance ranges were wide (large differences in Al) and narrow (small differences in AI). Results: Speech clarity judgments obtained by paired comparisons and category rating were highly related to the Al estimates both for normal-hearing and hearing-impaired subjects. When the performance range was wide, both methods provided similar judgments for the normal-hearing subjects. For the hearing-impaired subjects, paired comparisons were more sensitive than category rating. When the performance range was narrow, paired comparisons were more sensitive than category rating in differentiating between filters for both groups of subjects. This difference was less obvious for the normal-hearing subjects when paired comparison data were converted to a scale comparable to the category ratings. Large between-subject variability was evident for the hearing-impaired subjects on the psychophysical scaling procedures, most notably for category rating. Conclusions: When judging the clarity among stimulus items where performance varied over a wide range, both category rating and paired comparisons provided comparable judgments for normal-hearing listeners. For conditions in which perceptual differences between stimulus items were restricted either by the choice of conditions or by the effects of sensorineural hearing loss, the method of paired comparisons was the more sensitive procedure. C1 UNIV CALIF LOS ANGELES,SCH MED,DIV HEAD & NECK SURG,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,DEPT BIOMATH,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. FU NIDCD NIH HHS [5 K08 DC 00083-04] NR 48 TC 22 Z9 22 U1 3 U2 5 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0196-0202 J9 EAR HEARING JI Ear Hear. PD AUG PY 1997 VL 18 IS 4 BP 294 EP 306 DI 10.1097/00003446-199708000-00004 PG 13 WC Audiology & Speech-Language Pathology; Otorhinolaryngology SC Audiology & Speech-Language Pathology; Otorhinolaryngology GA XU406 UT WOS:A1997XU40600005 PM 9288475 ER PT J AU Merriam, GR Buchner, DM Prinz, PN Schwartz, RS Vitiello, MV AF Merriam, GR Buchner, DM Prinz, PN Schwartz, RS Vitiello, MV TI Potential applications of GH secretagogs in the evaluation and treatment of the age-related decline in growth hormone secretion SO ENDOCRINE LA English DT Article; Proceedings Paper CT Meeting on Assessment of the Growth Hormone/IGF-I Axis in Aging CY APR 14, 1997 CL BETHESDA, MD DE growth hormone (GH); GH-releasing hormone (GHRH); GH-releasing peptide (GHRP); insulin-like growth factor-I (IGF-I) ID FACTOR-I LEVELS; DEFICIENT CHILDREN; (GH)-RELEASING HORMONE; ELDERLY SUBJECTS; NORMAL MEN; OLD MEN; RESPONSES; ARGININE; ADULTS; STIMULATION AB The two classes of GH secretagogs-GH-releasing hormone (GHRH) and the GH-releasing peptides and their analogs (GHRP's)-retain their ability to endogenous GH secretion in healthy and frail elderly subjects. They have very limited utility in assessment of the state of the GH/IGF-I axis except to confirm an intact pituitary, but they are attractive potential alternatives to GH as therapeutic agents. There is wide interest in the possibility that elevating GH and IGF-I might increase muscle mass, physical strength and performance, and possibly sleep and cognition in aging, The GH secretagogs, like CH, can produce a sustained stimulation of this axis; in contrast to GH, they preserve feedback regulation at the pituitary level and stimulate a near-physiologic pulsatile pattern of GH release. GHRP's and their nonpeptide analogs are also active when given orally, a significant practical advantage. Short-term treatment studies have shown that GHRH and the GHRP's can enhance GH secretion and elevate IGF-I and IGFBP-3 levels; that GHRH may promote sleep; and that these agents are generally well tolerated, Longer-term studies assessing effects upon body composition and physical and psychological function are underway. C1 UNIV WASHINGTON, SCH MED, SEATTLE, WA USA. RP Merriam, GR (reprint author), VA PUGET SOUND HLTH CARE SYST, RES SERV 151, AMER LAKE DIV, BLDG 18S, ROOM 7, TACOMA, WA 98493 USA. OI Vitiello, Michael/0000-0002-9776-0473 NR 27 TC 11 Z9 11 U1 0 U2 1 PU HUMANA PRESS INC PI TOTOWA PA 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA SN 1355-008X EI 1559-0100 J9 ENDOCRINE JI Endocrine PD AUG PY 1997 VL 7 IS 1 BP 49 EP 52 DI 10.1007/BF02778062 PG 4 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA YJ962 UT WOS:A1997YJ96200007 PM 9449031 ER PT J AU Yin, A Miraglia, S Magee, K Zanjani, E Ogawa, M Buck, DW AF Yin, A Miraglia, S Magee, K Zanjani, E Ogawa, M Buck, DW TI AC133, a novel monoclonal antibody to human hematopoietic stem and progenitor cells SO EXPERIMENTAL HEMATOLOGY LA English DT Meeting Abstract C1 AMCORP CORP,SUNNYVALE,CA. UNIV NEVADA,RENO,NV 89557. RALPH H JOHNSON VET ADM MED CTR,CHARLESTON,SC. NR 0 TC 2 Z9 2 U1 0 U2 1 PU CARDEN JENNINGS PUBL CO LTD PI CHARLOTTESVILLE PA BLAKE CTR, STE 200, 1224 W MAIN ST, CHARLOTTESVILLE, VA 22903 SN 0301-472X J9 EXP HEMATOL JI Exp. Hematol. PD AUG PY 1997 VL 25 IS 8 BP 523 EP 523 PG 1 WC Hematology; Medicine, Research & Experimental SC Hematology; Research & Experimental Medicine GA XR330 UT WOS:A1997XR33000508 ER PT J AU Gralnek, IM Jensen, DM Kovacs, TOG Jutabha, R Jensen, ME Cheng, S Gornbein, J Freeman, ML Machicado, GA Smith, J Sue, M Kominski, G AF Gralnek, IM Jensen, DM Kovacs, TOG Jutabha, R Jensen, ME Cheng, S Gornbein, J Freeman, ML Machicado, GA Smith, J Sue, M Kominski, G TI An economic analysis of patients with active arterial peptic ulcer hemorrhage treated with endoscopic heater probe, injection sclerosis, or surgery in a prospective, randomized trial SO GASTROINTESTINAL ENDOSCOPY LA English DT Article; Proceedings Paper CT Annual Meeting of the American-Society-for-Gastrointestinal-Endoscopy CY MAY 14-18, 1995 CL SAN DIEGO, CA SP Amer Soc Gastrointestinal Endoscopy ID UPPER GASTROINTESTINAL-TRACT; PURE ALCOHOL INJECTION; MULTIPOLAR ELECTROCOAGULATION; UNITED-STATES; THERAPY; THERMOCOAGULATION; COST; HEMOSTASIS; DISEASE; LASER AB Background: There are no published, detailed assessments of the direct costs of endoscopic hemostasis for actively bleeding peptic ulcers. We compared the direct costs of care for patients with active ulcer hemorrhage treated with endoscopic or medical-surgical therapies and correlated these costs with patient outcomes. Methods: In a prospective, randomized, controlled trial, 31 patients with active ulcer hemorrhage at emergency endoscopy were randomly assigned to heater probe, injection, or medical-surgical treatment. For further ulcer bleeding, heater probe and injection patients were re-treated endoscopically and medical-surgical patients were referred for surgery. Direct costs were estimated using fixed and variable costs for resources consumed and Medicare reimbursement rates for physician fees. Results: Compared to medical-surgical treatment, the heater probe and injection groups had significantly higher primary hemostasis rates (100% and 90% vs 8%) and lower rates of emergency surgery (0% and 10% vs 75%), blood transfusions, and median direct costs per patient ($4153 and $5247 vs $11,149). Furthermore, compared to medical-surgical treatment, the heater probe group had a significantly lower incidence of severe ulcer rebleeding (11% vs 75%). Conclusions: Heater probe and injection sclerosis are similarly efficacious treatments for active ulcer hemorrhage, and both treatments yield significantly lower direct costs of medical care and cost savings. C1 W LOS ANGELES VET AFFAIRS MED CTR,CURE DIGEST DIS RES CTR,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,DEPT BIOMATH,LOS ANGELES,CA 90024. HENNEPIN CTY MED CTR,MINNEAPOLIS,MN 55415. NORTHRIDGE HOSP MED CTR,NORTHRIDGE,CA. ALTON OCHSNER MED FDN & OCHSNER CLIN,NEW ORLEANS,LA 70121. WHITE MEM MED CTR,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,SCH PUBL HLTH,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,CTR HLTH POLICY RES,LOS ANGELES,CA 90024. RP Gralnek, IM (reprint author), UNIV CALIF LOS ANGELES,SCH MED,DIV DIGEST DIS,CTR HLTH SCI,DEPT MED,CHS 44-138,10833 LECONTE AVE,LOS ANGELES,CA 90095, USA. RI smith, james/C-9922-2016 FU NIADDK NIH HHS [R0I-AM33273] NR 42 TC 46 Z9 46 U1 2 U2 2 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0016-5107 J9 GASTROINTEST ENDOSC JI Gastrointest. Endosc. PD AUG PY 1997 VL 46 IS 2 BP 105 EP 112 DI 10.1016/S0016-5107(97)70056-7 PG 8 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA XU280 UT WOS:A1997XU28000001 PM 9283858 ER PT J AU Jarvis, BW Lichenstein, H Qureshi, N AF Jarvis, BW Lichenstein, H Qureshi, N TI Diphosphoryl lipid A from Rhodobacter sphaeroides inhibits complexes that form in vitro between lipopolysaccharide (LPS)-binding protein, soluble CD14, and spectrally pure LPS SO INFECTION AND IMMUNITY LA English DT Article ID BINDING-PROTEIN; IDENTIFICATION; RECOGNITION; ANTAGONIZE; ENDOTOXINS; ATCC-17023 AB An early event in septic shock is the activation of macrophages by a complex consisting of lipopolysaccharide (LPS), LPS-binding protein (LBP), and the cell surface antigen CD14, The complexes that form between [H-3]ReLPS (ReLPS is deep-rough-chemotype hexacyl LPS from B. coli D31m4), soluble CD14 (sCD14), and LBP were analyzed by two independent methods, native (nondenaturing) gel electrophoresis and size-exclusion high-performance liquid chromatography (HPLC), This is the first reported use of HPLC to purify and study LPS-protein complexes, The binding of [H-3]ReLPS to LBP and sCD14 was inhibited by preincubation with diphosphoryl lipid A from Rhodobacter sphaeroides (RsDPLA), a potent LPS antagonist, In addition, [3H]ReLPS bound to LBP and to a truncated form of sCD14 [sCD14((1-152))] that contained the LPS binding domain. Binding to both proteins was blocked by RsDPLA. Thus, RsDPLA competes in a 1:1 ratio for the same or nearby binding sites on ReLPS complexes, Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of aggregated ReLPS eluting from the HPLC indicated that only LBP not sCD14, was bound to the aggregated ReLPS. This finding supports the binary model of LPS complex formation with LBP and sCD14. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,LAB MYCOBACTERIOL,MADISON,WI 53705. UNIV WISCONSIN,DEPT BACTERIOL,MADISON,WI 53705. AMGEN INC,BOULDER,CO 80301. FU NIGMS NIH HHS [GM-50870] NR 24 TC 33 Z9 33 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD AUG PY 1997 VL 65 IS 8 BP 3011 EP 3016 PG 6 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA XM714 UT WOS:A1997XM71400003 PM 9234747 ER PT J AU Stock, JL Avioli, LV Baylink, DJ Chesnut, C Genant, HK Maricic, MJ Silverman, SL Schaffer, AV Feinblatt, J AF Stock, JL Avioli, LV Baylink, DJ Chesnut, C Genant, HK Maricic, MJ Silverman, SL Schaffer, AV Feinblatt, J TI Calcitonin-salmon nasal spray reduces the incidence of new vertebral fractures in postmenopausal women: Three-year interim results of the proof study. SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract C1 WASHINGTON UNIV,SCH MED,ST LOUIS,MO. MEM HLTH CARE,WORCESTER,MA. LOMA LINDA UNIV,LOMA LINDA,CA 92350. UNIV WASHINGTON,MED CTR,SEATTLE,WA 98195. UNIV CALIF SAN FRANCISCO,SAN FRANCISCO,CA 94143. UNIV ARIZONA,HLTH SCI CTR,TUCSON,AZ. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. NOVARTIS PHARMACEUT,E HANOVER,NJ. NR 0 TC 1 Z9 1 U1 0 U2 1 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 SN 0884-0431 J9 J BONE MINER RES JI J. Bone Miner. Res. PD AUG PY 1997 VL 12 SU 1 BP 187 EP 187 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XP627 UT WOS:A1997XP62700186 ER PT J AU Greenspan, SL Bouxsein, ML Melton, ME Kolodny, AH Clair, JH Delucca, PT Stek, M Faulkner, KG Orwoll, ES AF Greenspan, SL Bouxsein, ML Melton, ME Kolodny, AH Clair, JH Delucca, PT Stek, M Faulkner, KG Orwoll, ES TI Precision and discriminatory ability of calcaneal bone assessment technologies SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Article; Proceedings Paper CT 2nd Annual Scientific Meeting of the Society-for-Clinical-Densitometry CY JAN 18-21, 1996 CL COLORADO SPRINGS, CO SP Soc Clin Densitometry ID X-RAY ABSORPTIOMETRY; BAND ULTRASOUND ATTENUATION; HIP FRACTURE; POSTMENOPAUSAL WOMEN; MINERAL DENSITY; RISK-FACTORS; QUANTITATIVE ULTRASOUND; SKELETAL SITES; ELDERLY WOMEN; OS CALCIS AB To determine if measuring skeletal status at the calcaneus is a potentially valuable technique for diagnosing osteoporosis, we examined five calcaneal assessment techniques in 53 young normal women and 108 postmenopausal women with osteoporosis and compared these measurements to dual-energy X-ray absorptiometry (DEXA)) at the calcaneus, hip, and spine. The five instruments, including single-energy X-ray absorptiometry (SEXA) and four quantitative ultrasound (QUS) instruments, were evaluated for precision, ability to discriminate osteoporotic from young normal subjects, and correlation to the other instruments, The coefficient of variation (%CV) for instrument, positioning, interobserver, and short-term precision of the five calcaneal instruments ranged from 1.34-7.76%, 1.63-7.00%, 1.84-9.44%, and 1.99-7.04%, respectively, The %CVs for positioning, interobserver, and short-term precision were similar for calcaneal DEXA, calcaneal SEXA, and stiffness (as measured by Achilles), The %CVs for instrument precision were similar between calcaneal DEXA and SEXA. The ability of the five calcaneal instruments to discriminate osteoporotic from young normal subjects was similar based on the analysis of area under the receiver operating characteristic curves (range 0.88-0.93) and equivalent to DEXA of the calcaneus and hip (0.88-0.93), The correlations between the measurements of five calcaneal instruments were strong (0.80 less than or equal to r less than or equal to 0.91,p < 0.001), These data suggest that although the precision is variable? the calcaneal QUS and SEXA instruments can discriminate between osteoporotic patients and young normal controls and appear to be a useful technique for assessment of osteoporosis. C1 MERCK & CO INC, W POINT, PA 19486 USA. BETH ISRAEL DEACONESS MED CTR, OSTEOPOROSIS PREVENT & TREATMENT CTR, BOSTON, MA USA. BETH ISRAEL DEACONESS MED CTR, ORTHOPAED BIOMECH LAB, BOSTON, MA USA. OREGON OSTEOPOROSIS CTR, PORTLAND, OR USA. PORTLAND VA MED CTR, BONE & MINERAL RES UNIT, PORTLAND, OR USA. OREGON HLTH SCI UNIV, PORTLAND, OR 97201 USA. OI Orwoll, Eric/0000-0002-8520-7355 NR 41 TC 142 Z9 144 U1 1 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0884-0431 EI 1523-4681 J9 J BONE MINER RES JI J. Bone Miner. Res. PD AUG PY 1997 VL 12 IS 8 BP 1303 EP 1313 DI 10.1359/jbmr.1997.12.8.1303 PG 11 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XN797 UT WOS:A1997XN79700020 PM 9258762 ER PT J AU Chen, S McDougall, S IidaKlein, A Lee, S Hahn, TJ AF Chen, S McDougall, S IidaKlein, A Lee, S Hahn, TJ TI ECM-mediated regulation of PTH/PTHrP receptor expression in rat chondrocytes. SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES,SCH MED,W LOS ANGELES VET AFFAIRS MED CTR,CTR GERIATR RES EDUC & CLIN,LOS ANGELES,CA 90073. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 SN 0884-0431 J9 J BONE MINER RES JI J. Bone Miner. Res. PD AUG PY 1997 VL 12 SU 1 BP F275 EP F275 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XP627 UT WOS:A1997XP62700764 ER PT J AU Lim, T Lee, S Chen, S McDougall, S IidaKlein, A Hahn, TJ AF Lim, T Lee, S Chen, S McDougall, S IidaKlein, A Hahn, TJ TI The alpha v beta 3 vitronectin receptor: Second messenger generation and cell phenotype regulation in mammalian chondrocytes. SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES,SCH MED,W LOS ANGELES VET AFFAIRS MED CTR,CTR GERIATR RES EDUC & CLIN,LOS ANGELES,CA 90073. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 SN 0884-0431 J9 J BONE MINER RES JI J. Bone Miner. Res. PD AUG PY 1997 VL 12 SU 1 BP F274 EP F274 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XP627 UT WOS:A1997XP62700763 ER PT J AU McDougall, S Chen, S Glackin, C Fang, MA AF McDougall, S Chen, S Glackin, C Fang, MA TI The role of basic fibroblast growth factor and EGR-1 in regulating alpha 2(I) collagen and twist gene expression in osteoblasts. SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES,SCH MED,W LOS ANGELES VET AFFAIRS MED CTR,CTR GERIATR RES EDUC & CLIN,LOS ANGELES,CA 90073. CITY HOPE NATL MED CTR,DUARTE,CA 91010. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 SN 0884-0431 J9 J BONE MINER RES JI J. Bone Miner. Res. PD AUG PY 1997 VL 12 SU 1 BP F215 EP F215 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XP627 UT WOS:A1997XP62700704 ER PT J AU Menaa, C Roodman, GD AF Menaa, C Roodman, GD TI Increased sensitivity to 1,25-(OH)(2)D-3 of osteoclast precursors in Paget's disease is not due to differences in expression levels of the 1,25-D receptor. SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 SN 0884-0431 J9 J BONE MINER RES JI J. Bone Miner. Res. PD AUG PY 1997 VL 12 SU 1 BP F418 EP F418 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XP627 UT WOS:A1997XP62700907 ER PT J AU Peters, JH Lee, S McDougall, S Hahn, TJ AF Peters, JH Lee, S McDougall, S Hahn, TJ TI Rat chondrocytes interact with multiple segments of fibronectin. SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES,SCH MED,W LOS ANGELES VET AFFAIRS MED CTR,GRECC,LOS ANGELES,CA 90073. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 SN 0884-0431 J9 J BONE MINER RES JI J. Bone Miner. Res. PD AUG PY 1997 VL 12 SU 1 BP F273 EP F273 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XP627 UT WOS:A1997XP62700761 ER PT J AU Choi, SJ Devlin, RD Reddy, SV Roodman, GD AF Choi, SJ Devlin, RD Reddy, SV Roodman, GD TI Cloning and identification of a novel inhibitor of osteoclast formation and bone resorption derived from an osteoclast library. SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 SN 0884-0431 J9 J BONE MINER RES JI J. Bone Miner. Res. PD AUG PY 1997 VL 12 SU 1 BP P217 EP P217 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XP627 UT WOS:A1997XP62700211 ER PT J AU Yamaguchi, DT Ma, D Huang, J Kim, T Ajmera, A AF Yamaguchi, DT Ma, D Huang, J Kim, T Ajmera, A TI Inhibition of gap junction communication in osteoblastic cells by electromagnetic fields is dependent on intracellular pH. SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,GRECC,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,RES SERV,LOS ANGELES,CA 90073. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 SN 0884-0431 J9 J BONE MINER RES JI J. Bone Miner. Res. PD AUG PY 1997 VL 12 SU 1 BP S248 EP S248 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XP627 UT WOS:A1997XP62701243 ER PT J AU Feliers, D Woodruff, K Abboud, S AF Feliers, D Woodruff, K Abboud, S TI Role of insulin-like growth factor binding protein (IGFBP)-4 in decreasing bone formation in multiple myeloma (MM). SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 SN 0884-0431 J9 J BONE MINER RES JI J. Bone Miner. Res. PD AUG PY 1997 VL 12 SU 1 BP T410 EP T410 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XP627 UT WOS:A1997XP62700405 ER PT J AU Peterson, WJ Yamaguchi, DT AF Peterson, WJ Yamaguchi, DT TI Down-regulation of DNA synthesis during osteogenesis in vitro: The effect of cell density and the extracellular matrix. SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,GRECC,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA 90073. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 SN 0884-0431 J9 J BONE MINER RES JI J. Bone Miner. Res. PD AUG PY 1997 VL 12 SU 1 BP T397 EP T397 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XP627 UT WOS:A1997XP62700391 ER PT J AU Ohta, K Pang, XP Berg, L Hershman, JM AF Ohta, K Pang, XP Berg, L Hershman, JM TI Growth inhibition of new human thyroid carcinoma cell lines by activation of adenylate cyclase through the beta-adrenergic receptor SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Article; Proceedings Paper CT 69th Annual Meeting of the American-Thyroid-Association CY NOV 13-17, 1996 CL SAN DIEGO, CA SP Amer Thyroid Assoc ID MOLECULAR-CLONING; THYROTROPIN RECEPTOR; TRANSCRIPTION; EXPRESSION; DIFFERENTIATION; HOMEODOMAIN; SEQUENCE; PROTEIN; BINDING; TTF-1 AB In normal thyroid cells, the TSH-adenylate cyclase system plays a pivotal role in controlling growth and differentiation. However, the role of this system in the growth of thyroid carcinoma is not well understood. To investigate this subject, we have established four new human thyroid carcinoma cell lines, designated BHP 2-7, 7-13, 10-3, and 18-21, from different patients. Northern gel analysis revealed that all of these cell lines expressed Pax-8 messenger ribonucleic acid; additionally, only BHP 18-21 cells expressed TTF-1 messenger ribonucleic acid. These cells were treated with various concentrations of 8-bromo-cAMP, forskolin, TSH, and adrenergic receptor agonist (norepinephrine, epinephrine, and isoproterenol), Cell proliferation was assessed by [H-3]thymidine incorporation and cell number. In these human thyroid carcinoma cell lines, the addition of 8-bromo-cAMP reduced [H-3]thymidine incorporation at a concentration of 10 mu mol/L. Forskolin (0.1-10 mu mol/L) significantly induced cAMP accumulation, decreased [H-3]thymidine incorporation, and reduced cell number in a dose-dependent manner. Conversely, TSH (0.01-1 mU/mL) did not affect the accumulation of cAMP or cell growth. We found that adrenergic receptor agonists induced the accumulation of cAMP and inhibited cell growth. The rank of potency was isoproterenol > epinephrine >> norepinephrine. The binding studies of [H-3]CGP-12177, a specific beta-adrenergic agonist, revealed that these new thyroid carcinoma cells had beta-adrenergic receptors. These results indicate that cAMP inhibits the growth of some human thyroid carcinoma cells, and that cAMP production is regulated through beta-adrenergic receptor-mediated pathways, but not through TSH receptor-mediated pathways. C1 W LOS ANGELES VET AFFAIRS MED CTR, DIV ENDOCRINOL 111D, ENDOCRINE RES LAB, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, SCH MED, LOS ANGELES, CA 90073 USA. FU NCI NIH HHS [CA-61863] NR 28 TC 54 Z9 54 U1 2 U2 2 PU ENDOCRINE SOC PI WASHINGTON PA 2055 L ST NW, SUITE 600, WASHINGTON, DC 20036 USA SN 0021-972X EI 1945-7197 J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD AUG PY 1997 VL 82 IS 8 BP 2633 EP 2638 DI 10.1210/jc.82.8.2633 PG 6 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XP098 UT WOS:A1997XP09800043 PM 9253346 ER PT J AU Ford, JD Fisher, P Larson, L AF Ford, JD Fisher, P Larson, L TI Object relations as a predictor of treatment outcome with chronic posttraumatic stress disorder SO JOURNAL OF CONSULTING AND CLINICAL PSYCHOLOGY LA English DT Article ID COLLABORATIVE RESEARCH-PROGRAM; PERSONALITY-DISORDERS; VIETNAM VETERANS; DYNAMIC PSYCHOTHERAPY; CASE FORMULATION; DEPRESSION; SEVERITY; IMPACT; DISSOCIATION; RELIABILITY AB The role of object relations as a predictor of outcome was evaluated in inpatient posttraumatic stress disorder( PTSD) treatment. Cohort outcome at discharge on psychometric indices was mixed, with limited evidence of reliable or clinically significant change. Treatment was associated with an overall reduction in utilization of inpatient psychiatric and residential domiciliary services. However, moderate (vs. low) levels of object relations were predictive of reliable change outcome, independent of demographics, Axis II diagnosis, symptomatic severity, or early childhood or war zone trauma exposure. The findings suggest that consideration should be given both to the manner in which patients seeking treatment for PTSD are screened and matched with a range of treatment or rehabilitation services and to how treatment outcome is conceptualized beyond symptom reduction. Rehabilitation of chronic posttraumatic symptomatology and associated psychosocial impairment may be facilitated by assessment, treatment design, and client-treatment matching on the basis of multidimensional psychological indices. C1 DARTMOUTH COLL, SCH MED, HANOVER, NH 03755 USA. OREGON SOCIAL LEARNING CTR, EUGENE, OR 97401 USA. PORTLAND VET AFFAIRS MED CTR, PORTLAND, OR USA. RP Ford, JD (reprint author), DEPT VET AFFAIRS, EXECUT DIV, NATL CTR PTSD, VET AFFAIRS MED CTR 116D, WHITE RIVER JCT, VT 05009 USA. NR 72 TC 40 Z9 41 U1 4 U2 10 PU AMER PSYCHOLOGICAL ASSOC PI WASHINGTON PA 750 FIRST ST NE, WASHINGTON, DC 20002-4242 USA SN 0022-006X EI 1939-2117 J9 J CONSULT CLIN PSYCH JI J. Consult. Clin. Psychol. PD AUG PY 1997 VL 65 IS 4 BP 547 EP 559 PG 13 WC Psychology, Clinical SC Psychology GA XM674 UT WOS:A1997XM67400003 PM 9256555 ER PT J AU Li, GQ Salhany, KE Rook, AH Lessin, SR AF Li, GQ Salhany, KE Rook, AH Lessin, SR TI The pathogenesis of large cell transformation in cutaneous T-cell lymphoma is not associated with t(2;5)(p23;q35) chromosomal translocation SO JOURNAL OF CUTANEOUS PATHOLOGY LA English DT Article ID POLYMERASE CHAIN-REACTION; NON-HODGKINS-LYMPHOMA; MYCOSIS-FUNGOIDES; DISEASE; RECEPTOR; PROTEIN; GENE; CLASSIFICATION; EXPRESSION; AMERICAN AB In 20%-50% of the advanced cutaneous T-cell lymphomas (CTCL), malignant T cells undergo large cell transformation (LCT). The malignant T cells of LCT in CTCL can share morphologic and immunophenotypic similarities with CD30 (Ki-1)-positive anaplastic lar-ge cell lymphoma (ALCL), suggesting a common mechanism of pathogenesis. The t(2;5) (p23;q35) translocation, resulting in the fusion of the nucleophosmin (NPM) gene and the anaplastic lymphoma kinase (ALK) gene, is associated with primary CD30+ ALCL. To determine whether acquisition of this chromosomal translocation is involved in the pathogeneis of LCT in CTCL, we examined 12 tumor samples from 9 CTCL patients, including 8 with LCT-CTCL and one with concurrent CTCL and Hodgkin's disease, for the presence of the t(2;5) translocation. Numerous CD30+ large cells were present in 4 LCT-CTCL consistent with secondary CD30+ ALCL; CD30 was expressed by <10% of the large cells in another case and was negative in the other 3 lymphomas. Using primers spanning the NPM/ALK fusion junction, PCR amplification following reverse transcription (RT) of mRNA failed to show the products of NPM/ALK fusion in all samples tested. Thus, the t(2;5) (p23;q35) translocation does not appear to be involved in the molecular pathogenesis of LCT in CTCL, including CD30+ cases. (C) Munksgaard 1997. C1 UNIV PENN,MED CTR,DEPT DERMATOL,PHILADELPHIA,PA 19104. UNIV PENN,MED CTR,DEPT PATHOL & LAB MED,PHILADELPHIA,PA 19104. VET AFFAIRS MED CTR,PHILADELPHIA,PA. FU NCI NIH HHS [R01 CA-58841, R29 CA-55017]; NIAMS NIH HHS [T32 AR-07565] NR 35 TC 18 Z9 18 U1 0 U2 0 PU MUNKSGAARD INT PUBL LTD PI COPENHAGEN PA 35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK SN 0303-6987 J9 J CUTAN PATHOL JI J. Cutan. Pathol. PD AUG PY 1997 VL 24 IS 7 BP 403 EP 408 DI 10.1111/j.1600-0560.1997.tb00814.x PG 6 WC Dermatology; Pathology SC Dermatology; Pathology GA XP876 UT WOS:A1997XP87600003 PM 9274957 ER PT J AU Verma, RC Wu, HM Duerinckx, AJ Landowski, L Schiepers, C Rooholamini, SA AF Verma, RC Wu, HM Duerinckx, AJ Landowski, L Schiepers, C Rooholamini, SA TI Picture archiving and communication system-asynchronous transfer mode network in a midsized hospital SO JOURNAL OF DIGITAL IMAGING LA English DT Article; Proceedings Paper CT 14th Symposium for Computer Applications in Radiology CY JUN 21-24, 1997 CL MAYO CLIN, ROCHESTER, MN SP Soc Comp Applicat Radiol HO MAYO CLIN DE PACS; LAN; WAN; ATM AB This article describes the pathway to full implementation of a hospital information system-picture archiving and communication system-wide area network (HIS-PACS-WAN) in a 300-bed acute care hospital, and the linking of th at system to two other off-site medical centers. The PACS included direct digital capture of computed tomography (CT), magnetic resonance (MR) imaging, nuclear medicine, and ultrasonography images into an Olicon archive. Plain radiographs and fluoroscopy images were digitized manually and archived into an Olicon system. The active archive included current images on each Olicon workstation and the juke box. Long-term archiving of the images on removable optical discs, which would be loaded manually by an operator every time a request for one of these studies appeared on the operator's monitor, also was implemented. Ability to store, retrieve, and display simultaneously the physician's report of each procedure along with the images was an ultimate goal. The WAN is to be used for teleradiology and teleconferencing among the three medical centers involved in this study as well as other off-site locations. Phase I included the design and installation of the local area network (LAN) in the Department of Radiology at Olive View-UCLA Medical Center. This included the clinics and the inpatient and hospital-wide fiber-optic network and its linkage to the local telephone company. Phase II involved linkage of the Olicon workstations to imaging equipment. This implementation has been delayed significantly because of inadequate needs assessment, absence of planning for forward-compatibility to imaging equipment, and incompatibilities in DICOM conformance among vendors. Every PACS project must include an in-depth needs analysis, which should be updated yearly because of rapid turnover of technology. Although this analysis should have a heavy emphasis on clinical needs, it must incorporate the hospital-wide needs for an integrated information systems network. Integration of PACS, HIS, RIS, and a dictation/transcription system is a complex task that requires a full-time, clinically oriented project officer for successful completion. Copyright (C) 1997 by W.B. Saunders Company. C1 W LOS ANGELES VET AFFAIRS MED CTR,SERV RADIOL,LOS ANGELES,CA 90073. RP Verma, RC (reprint author), OLIVE VIEW UCLA MED CTR,DEPT RADIOL SCI,2C 176,14445 OLIVE VIEW DR,SYLMAR,CA 91342, USA. NR 0 TC 2 Z9 2 U1 0 U2 3 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0897-1889 J9 J DIGIT IMAGING JI J. Digit. Imaging PD AUG PY 1997 VL 10 IS 3 SU 1 BP 99 EP 102 PG 4 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA XR542 UT WOS:A1997XR54200034 PM 9268851 ER PT J AU Teslow, TN AF Teslow, TN TI The laser film digitizer: Density, contrast, and resolution SO JOURNAL OF DIGITAL IMAGING LA English DT Article; Proceedings Paper CT 14th Symposium for Computer Applications in Radiology CY JUN 21-24, 1997 CL MAYO CLIN, ROCHESTER, MN SP Soc Comp Applicat Radiol HO MAYO CLIN DE quality assurance; image quality; laser film digitizer; film densitometry; PACS; laser scanner; digital radiography ID TEST PATTERN; DISPLAYS AB This work evaluates differences in density linearity, contrast, and resolution of digital images acquired from a laser film scanner. A calibrated photographic step tablet tested linearity of diffuse optical density (OD). It was digitized within center openings of a dark and light film. The 14- x 17-inch films were scanned into 1024 x 1280 picture elements of 8 bits. A photographic tapered resolution guide evaluated the scanner's detail resolution. A single characteristic curve adequately represents the rate of change of OD per pixel value, but two piecewise linear functions are better. One ranges in light OD and a second in dark OD. Contrast shows a steeper slope in a light film rather than a dark film, yet a greater difference exists between two identical digitizers. The resolution test diminishes at 1.3 line pairs per mm (LP/mm), which compares to a Nyquist limit of 1.44 LP/mm. Such density, contrast, and resolution tests yield a benchmark to assure quality operations. Copyright (C) 1997 by W.B. Saunders Company. C1 DEPT VET AFFAIRS MED CTR,RADIOTHERAPY SERV,HOUSTON,TX. RP Teslow, TN (reprint author), BAYLOR COLL MED,DEPT RADIOL,1 BAYLOR PLAZA,HOUSTON,TX 77030, USA. NR 9 TC 3 Z9 4 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0897-1889 J9 J DIGIT IMAGING JI J. Digit. Imaging PD AUG PY 1997 VL 10 IS 3 SU 1 BP 128 EP 132 PG 5 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA XR542 UT WOS:A1997XR54200040 PM 9268857 ER PT J AU Protopapas, Z Siegel, EL Scanlon, M Mazan, W Reiner, BI Ghabrekidan, H Warner, J Pomerantz, SM Gitlin, JN AF Protopapas, Z Siegel, EL Scanlon, M Mazan, W Reiner, BI Ghabrekidan, H Warner, J Pomerantz, SM Gitlin, JN TI Experience with comparative picture archiving and communication system baseline data collection at four Veterans Affairs Medical Centers: Methodology, lessons learned, and suggestions for improvement SO JOURNAL OF DIGITAL IMAGING LA English DT Article; Proceedings Paper CT 14th Symposium for Computer Applications in Radiology CY JUN 21-24, 1997 CL MAYO CLIN, ROCHESTER, MN SP Soc Comp Applicat Radiol HO MAYO CLIN C1 VET AFFAIRS MED CTR,DEPT DIAGNOST RADIOL,BALTIMORE,MD 21201. UNIV MARYLAND,SCH MED,BALTIMORE,MD 21201. VET AFFAIRS MED CTR,PHILADELPHIA,PA. JOHNS HOPKINS SCH MED,BALTIMORE,MD. NR 2 TC 2 Z9 2 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0897-1889 J9 J DIGIT IMAGING JI J. Digit. Imaging PD AUG PY 1997 VL 10 IS 3 SU 1 BP 161 EP 164 PG 4 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA XR542 UT WOS:A1997XR54200051 PM 9268868 ER PT J AU Scremin, OU Li, MG Jenden, DJ AF Scremin, OU Li, MG Jenden, DJ TI Cholinergic modulation of cerebral cortical blood flow changes induced by trauma SO JOURNAL OF NEUROTRAUMA LA English DT Article; Proceedings Paper CT 13th Annual Houston Conference on Biomedical Engineering Research CY FEB 09, 1996 CL HOUSTON, TX DE cerebrovascular circulation; brain injuries; acetylcholine; physostigmine; scopolamine ID COMPRESSION CONTUSION TRAUMA; PERCUSSION BRAIN INJURY; SEVERE HEAD-INJURY; CHOLINESTERASE INHIBITOR; INTRACRANIAL-PRESSURE; WORKING MEMORY; AGED RATS; LONG-TERM; RESPONSES; ACETYLCHOLINE AB These experiments tested the role of cholinergic mechanisms in the changes of cerebral cortical blood flow (CBF) induced by brain trauma. CBF was measured with Iodo-C-14-antipyrine autoradiography, in 128 cerebral cortex regions of both hemispheres, distributed in eight coronal slices. The effects of a 6.3-mm diameter craniotomy over the left motor-sensory cortex with no weight drop, and of trauma (drop weight of 20 g from 30 cm height on left motor-sensory cortex through a 6.3 mm circular craniotomy) on CBF were studied at 2 and 24 h after the interventions. A group of control animals that received no intervention was also set up. Animals were treated with the cholinesterase inhibitor physostigmine salicylate (3.3 mu g/kg/min i.v. infusion started 60 min before CBF measurements), the cholinergic blocker scopolamine hydrobromide (1 mg/kg i.v. pulse, 18 min before CBF measurements), or with the drugs vehicle (saline). A focus of decreased CBF at the site of impact was observed 2 h after trauma, extending caudally as far as the occipital cortex. CBF on the contralateral cerebral cortex was also decreased. Both phenomena reversed partially at 24 h. This spontaneous recovery of CBF was blocked by scopolamine. Physostigmine reversed the decrease in CBF of the traumatized cortex, partially around the contused area and completely in more distant regions. The cerebral cortex contralateral to the trauma showed significantly higher CBF 24 h after trauma when compared to intact controls or craniotomy that peaked at the area symmetrical to the center of trauma. This phenomenon was also enhanced by physostigmine and completely blocked by scopolamine. These results suggest a prominent role of cholinergic mechanisms in the vascular adjustments that accompany cerebral trauma. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT PHYSIOL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,DEPT PHARMACOL & BIOMATH,LOS ANGELES,CA. RP Scremin, OU (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,RES DEPT,BLDG 115,ROOM 317,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 62 TC 17 Z9 17 U1 0 U2 1 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 0897-7151 J9 J NEUROTRAUM JI J. Neurotrauma PD AUG PY 1997 VL 14 IS 8 BP 573 EP 586 DI 10.1089/neu.1997.14.573 PG 14 WC Critical Care Medicine; Clinical Neurology; Neurosciences SC General & Internal Medicine; Neurosciences & Neurology GA XU894 UT WOS:A1997XU89400008 PM 9300567 ER PT J AU McNay, AT Ostad, A Moy, RL AF McNay, AT Ostad, A Moy, RL TI Surgical Pearl: Modified rhombic flap SO JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY LA English DT Article C1 UNIV CALIF LOS ANGELES,W LOS ANGELES VET ADM MED CTR,DIV DERMATOL,LOS ANGELES,CA 90024. NR 8 TC 3 Z9 3 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0190-9622 J9 J AM ACAD DERMATOL JI J. Am. Acad. Dermatol. PD AUG PY 1997 VL 37 IS 2 BP 256 EP 258 DI 10.1016/S0190-9622(97)80134-X PN 1 PG 3 WC Dermatology SC Dermatology GA XT275 UT WOS:A1997XT27500015 PM 9270513 ER PT J AU Lehmann, KG Ferris, ST HeathLange, SJ AF Lehmann, KG Ferris, ST HeathLange, SJ TI Maintenance of hemostasis after invasive cardiac procedures: Implications for outpatient catheterization SO JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY LA English DT Article ID PERIPHERAL VASCULAR COMPLICATIONS; CONVENTIONAL MANUAL COMPRESSION; ARTERIAL PUNCTURE SITES; RANDOMIZED TRIAL; CORONARY ANGIOPLASTY; STENT IMPLANTATION; GUIDING CATHETERS; BRACHIAL APPROACH; ANGIOGRAPHY; DEVICE AB Objectives. This study investigated the efficacy of four different methods of arterial puncture site management during recovery from invasive cardiac procedures, The primary goals were less patient discomfort and improved clinical outcome, Background The increasing use of outpatient catheterization, large interventional devices and potent periprocedural anticoagulation regimens has made the reduction of groin complications a high priority, Despite these trends, there are no randomized trials comparing commonly used techniques in treating the catheter entry site for the first few hours after the procedure, Methods. Four-hundred consecutive patients undergoing catheterization laboratory procedures were randomly assigned to one of four dressing techniques applied after achieving hemostasis: a sandbag placed over the site; a pressure dressing constructed from surgical gauze and elastic tape; a commercially available compression device; and no use of compressive dressing, Of these 400 patients, 171 would have been eligible for outpatient procedures in the absence of geographic constraints, The dressings were removed, and ambulation was encouraged 5 h after sheath removal. Uniform initial compression times, patient instructions, nursing follow-up and a structured interview and physical examination at 24 h were used, Results. The level of patient discomfort before and after dressing removal, as well as site tenderness at 24-h follow-up, was statistically similar in all four groups, Hematomas (typically small) and areas of ecchymosis were observed in 58 and 122 patients, respectively, but both their frequency and size were equally represented in each group, Important adverse events were confined to bleeding, rated as mild in 5.8%, moderate in 0.8% and severe in 0.6% of patients, Again, all four groups were statistically similar, Comparable findings were observed in the subgroup of patients eligible for outpatient procedures, Conclusions, Despite an increase in inconvenience and expense, none of the three compression techniques that were investigated improved patient satisfaction or outcome, Therefore, the routine use of compression dressings after invasive cardiac procedures cannot be recommended. (C) 1997 by the American College of Cardiology. C1 UNIV WASHINGTON,SCH MED,SEATTLE,WA 98108. RP Lehmann, KG (reprint author), VET AFFAIRS PUGET SOUND HLTH CARE SYST,SECT CARDIOL 111C,1660 S COLUMBIAN WAY,SEATTLE,WA 98108, USA. NR 35 TC 10 Z9 10 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0735-1097 J9 J AM COLL CARDIOL JI J. Am. Coll. Cardiol. PD AUG PY 1997 VL 30 IS 2 BP 444 EP 451 DI 10.1016/S0735-1097(97)00156-3 PG 8 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA XN171 UT WOS:A1997XN17100017 PM 9247517 ER PT J AU Rowe, CL Fleming, JO Nathan, MJ Sgro, JY Palmenberg, AC Baker, SC AF Rowe, CL Fleming, JO Nathan, MJ Sgro, JY Palmenberg, AC Baker, SC TI Generation of coronavirus spike deletion variants by high-frequency recombination at regions of predicted RNA secondary structure SO JOURNAL OF VIROLOGY LA English DT Article ID INTERTYPIC POLIOVIRUS RECOMBINANTS; MURINE HEPATITIS-VIRUS; QUASI-SPECIES NATURE; NEUROTROPIC CORONAVIRUS; MONOCLONAL-ANTIBODIES; CROSSOVER REGIONS; SEQUENCE-ANALYSIS; C VIRUS; GLYCOPROTEIN; PROTEIN AB Coronavirus RNA evolves in the central nervous systems (CNS) of mice during persistent infection. This evolution can be monitored by detection of a viral quasispecies of spike deletion variants (SDVs) (C. L. Rowe, S. C. Baker, M. J. Nathan, and J. O. Fleming, J. Virol. 71:2959-2969, 1997). We and others have Pound that the deletions cluster in the region from 1,200 to 1,800 nucleotides from the 5' end of the spike gene sequence, termed the ''hypervariable'' region. To address how SDVs might arise, we generated the predicted folding structures of the positive- and negative-strand senses of the entire 4,139-nt spike RNA sequence. We found that a prominent, isolated stem-loop structure is coincident with the hypervariable region in each structure, To determine if this predicted stem-loop is a ''hot spot'' for RNA recombination, Re assessed whether this region of the spike is more frequently deleted than three other selected regions of the spike sequence in a population of viral sequences isolated from the CNS of acutely and persistently infected mice. Using differential colony hybridization of cloned spike reverse transcription-PCR products, we detected SDVs in which the hot spot was deleted but did not detect SDVs in which other regions of the spike sequence were exclusively deleted. Furthermore, sequence analysis and mapping of the crossover sites of 25 distinct patterns of SDVs showed that the majority of crossover sites clustered to two regions at the base of the isolated stem-loop, which we designated as high-frequency recombination sites 1 and 2. Interestingly, the majority of the left and right crossover sites of the SDVs were directly across from or proximal to one another, suggesting that these SDVs are likely generated by intramolecular recombination. Overall, our results are consistent with there being an important role for the spike RNA secondary structure as a contributing factor in the generation of SDVs during persistent infection. C1 LOYOLA UNIV,STRITCH SCH MED,DEPT MICROBIOL & IMMUNOL,MAYWOOD,IL 60153. LOYOLA UNIV,STRITCH SCH MED,PROGRAM MOL BIOL,MAYWOOD,IL 60153. UNIV WISCONSIN,DEPT NEUROL,MADISON,WI 53792. UNIV WISCONSIN,DEPT MED MICROBIOL,MADISON,WI 53792. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI 53792. UNIV WISCONSIN,INST MOL VIROL,MADISON,WI 53706. UNIV WISCONSIN,DEPT ANIM HLTH & BIOMED SCI,MADISON,WI 53706. FU NIAID NIH HHS [AI32065] NR 52 TC 19 Z9 21 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD AUG PY 1997 VL 71 IS 8 BP 6183 EP 6190 PG 8 WC Virology SC Virology GA XK153 UT WOS:A1997XK15300058 PM 9223514 ER PT J AU Cohen, RM Feldman, GM Fernandez, PC AF Cohen, RM Feldman, GM Fernandez, PC TI The balance of acid, base and charge in health and disease SO KIDNEY INTERNATIONAL LA English DT Article ID RENAL TUBULAR-ACIDOSIS; LACTIC-ACIDOSIS; METABOLIC CONSEQUENCES; CITRATE EXCRETION; PROXIMAL COLON; DISTAL COLON; OXALATE; PH; KETOACIDOSIS; BICARBONATE C1 DEPT VET AFFAIRS MED CTR,RENAL SECT,PHILADELPHIA,PA 19104. ALLEGHENY UNIV HLTH SCI,GRAD HOSP,DIV NEPHROL,PHILADELPHIA,PA 19102. UNIV PENN,SCH MED,DIV NEPHROL,PHILADELPHIA,PA 19104. VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DIV NEPHROL,RICHMOND,VA 23298. FU NIDDK NIH HHS [DK-07006] NR 69 TC 23 Z9 23 U1 0 U2 2 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 SN 0085-2538 J9 KIDNEY INT JI Kidney Int. PD AUG PY 1997 VL 52 IS 2 BP 287 EP 293 DI 10.1038/ki.1997.334 PG 7 WC Urology & Nephrology SC Urology & Nephrology GA XP205 UT WOS:A1997XP20500001 PM 9263983 ER PT J AU Wray, NP Peterson, NJ Souchek, J Ashton, CM Hollingsworth, JC AF Wray, NP Peterson, NJ Souchek, J Ashton, CM Hollingsworth, JC TI Application of an analytic model to early readmission rates within the Department of Veterans Affairs SO MEDICAL CARE LA English DT Article DE quality of care; disease-outcome pairs; database analysis; administrative databases; readmission ID HOSPITAL DISCHARGE DATA; QUALITY; OUTCOMES; CARE; COMPLICATIONS; MORTALITY; DATABASES; ACCURACY; PAYMENT; BIAS AB OBJECTIVES. Adverse outcome rates are increasingly used as yardsticks for the quality of hospital care. However, the validity of many outcome studies has been undermined by the application of one outcome to all patients in large, diagnostically diverse populations, many of which lack evidence of a link between antecedent process of care and the rate of the outcome, the underlying assumption of the analysis. METHODS. TO address this analytic problem, the authors developed a model that improves the ability to identify quality problems because it selects diseases for which there are processes of care known to affect the outcome of interest. Thus, for these diseases, the outcome is most likely to be causally related to the antecedent care. In this study of hospital readmissions, risk-adjusted models were created for 17 disease categories with strong links between process and outcome. Using these models, we identified outlier hospitals. RESULTS. The authors hypothesized that if the model improved on identifying hospitals with quality of care problems, then outlier status would not be random. That is, hospitals found to have extreme rates in one year would be more likely to have extreme rates in subsequent years, and hospitals with extreme rates in one condition would be more likely to have extreme rates in related disease categories. It was hypothesized further that the correlation of outlier status across time and across diseases would be stronger in the 17 disease categories selected by the model than in 10 comparison disease categories with weak links between process and outcome. CONCLUSIONS. The findings support all these hypotheses. Although the present study shows that the model selects disease;outcome pairs where hospital outlier status is not random, the causal factors leading to outlier status could include (1) systematic unmeasured patient variation, (2) practice pattern variation that, although stable with time, is not indicative of substandard care, or (3) true quality-of-care problems. Primary data collection must be done to determine which of these three factors is most causally related to hospital outlier status. C1 BAYLOR COLL MED,DEPT MED,HOUSTON,TX 77030. RP Wray, NP (reprint author), HOUSTON VA MED CTR,VA HLTH SERV RES & DEV FIELD PROGRAM,2002 HOLCOMBE BLVD,HOUSTON,TX 77030, USA. NR 27 TC 16 Z9 16 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0025-7079 J9 MED CARE JI Med. Care PD AUG PY 1997 VL 35 IS 8 BP 768 EP 781 PG 14 WC Health Care Sciences & Services; Health Policy & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA XP851 UT WOS:A1997XP85100003 PM 9268250 ER PT J AU Leaf, DA Kleinman, MT Hamilton, M Barstow, TJ AF Leaf, DA Kleinman, MT Hamilton, M Barstow, TJ TI The effect of exercise intensity on lipid peroxidation SO MEDICINE AND SCIENCE IN SPORTS AND EXERCISE LA English DT Article DE lipid peroxidation; physical activity ID OXIDATIVE STRESS; PENTANE AB This study characterizes exercise-induced lipid peroxidation during graded aerobic exercise in seven healthy men and women (36.4 +/- 3 yr). Levels of ethane and pentane in expired breath during cardiopulmonary exercise stress testing were measured at rest, lactic acidosis threshold (LAT), maximal exercise ((V) over dot O-2max), and recovery. Serum malonaldehyde (MDA) levels were measured at rest before exercise and 5 min after maximal exercise. Expired ethane and pentane flux levels were increased above resting levels at LAT, continued to rise at (V) over dot O-2max, then declined during recovery. Serum MDA levels were not significantly different before and after maximal exercise. Substantial exercise-induced lipid peroxidation (by expired ethane and pentane) apparently occurred in healthy individuals at LAT and continued to increase at (V) over dot O-2max, yet rapidly attenuated during post-exercise recovery. These findings indicate that in healthy individuals physical exercise induced lipid peroxidation transiently and that there was a removal of lipid peroxidation byproducts during recovery. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90073. UNIV CALIF IRVINE,DEPT COMMUNITY & ENVIRONM MED,IRVINE,CA 92717. UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,DIV CARDIOL,LOS ANGELES,CA 90095. HARBOR UCLA MED CTR,DEPT MED,DIV RESP & CRIT CARE PHYSIOL MED,TORRANCE,CA 90509. RP Leaf, DA (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,DIV GEN INTERNAL MED,DEPT MED,WILSHIRE & SAWTELLE BLVDS,LOS ANGELES,CA 90073, USA. NR 21 TC 74 Z9 77 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0195-9131 J9 MED SCI SPORT EXER JI Med. Sci. Sports Exerc. PD AUG PY 1997 VL 29 IS 8 BP 1036 EP 1039 DI 10.1097/00005768-199708000-00008 PG 4 WC Sport Sciences SC Sport Sciences GA XR035 UT WOS:A1997XR03500008 PM 9268960 ER PT J AU Haake, DA Summers, TA McCoy, AM Schwartzman, W AF Haake, DA Summers, TA McCoy, AM Schwartzman, W TI Heat shock response and groEL sequence of Bartonella henselae and Bartonella quintana SO MICROBIOLOGY-UK LA English DT Article DE heat shock; Bartonella henselae; Bartonella quintana; GroEL ID SP-NOV; BACILLARY ANGIOMATOSIS; BRUCELLA-ABORTUS; COMB-NOV; ROCHALIMAEA; BACILLIFORMIS; PROTEINS; CELLS; PROPOSALS; PELIOSIS AB Transmission of Bartonella species from edoparasites to the mammalian host involves adaptation to thermal and other forms of stress. in order to better understand this process, the heat shock response of Bartonella henselae and Bartonella quintana was studied. Cellular proteins synthesized after shift to higher temperatures were intrinsically labelled with [S-35]methionine and analysed by gel electrophoresis and fluorography. The apparent molecular masses of three of the major heat shock proteins produced by the two Bartonella species were virtually identical, migrating at 70, 60 and 10 kDa. A fourth major shock protein was larger in B. quintana (20 kDa) than in B. henselae (17 kDa). The maximum heat shock response in B. quintana and B. henselae was observed at 39 degrees C and 42 degrees C, respectively. The groEL genes of both Bartonella species were amplified, sequenced and compared to other known groEL genes. The phylogenetic tree based on the groEL alignment places B. quintana and B. henselae in a monophyletic group with Bartonella bacilliformis. the deduced amino acid sequences of Bartonella GroEL homologues contain signature sequences that are uniquely shared by members of the Gram-negative alpha-purple subdivision of bacteria, which live within eukaryotic cells. Recombinant His(6)-GroEL fusion proteins were expressed in Escherichia coli to generate specific rabbit antisera. The GroEL antisera were used to confirm the identity of the 60 kDa Bartonella heat shock protein. These studies provide a foundation for evaluating the role of the heat shock response in the pathogenesis of Bartonella infection. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT IMMUNOL & MICROBIOL,LOS ANGELES,CA 90095. VET AFFAIRS MED CTR,DIV INFECT DIS,SEPULVEDA,CA 91343. RP Haake, DA (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,DIV INFECT DIS,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 36 TC 13 Z9 15 U1 0 U2 1 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING, BERKS, ENGLAND RG7 1AE SN 1350-0872 J9 MICROBIOL-UK JI Microbiology-(UK) PD AUG PY 1997 VL 143 BP 2807 EP 2815 PN 8 PG 9 WC Microbiology SC Microbiology GA XQ875 UT WOS:A1997XQ87500032 PM 9274034 ER PT J AU NevelMcGarvey, CA Levin, RM Hudson, AP AF NevelMcGarvey, CA Levin, RM Hudson, AP TI Transcription of mitochondrial and mitochondria-related nuclear genes in rabbit bladder following partial outlet obstruction SO MOLECULAR AND CELLULAR BIOCHEMISTRY LA English DT Article DE outlet obstruction; bladder; mitochondria; transcription; RNA ID URINARY-BLADDER; EXPRESSION; REGIONS; ACID AB Using the rabbit model, we showed that partial outlet obstruction of the urinary bladder causes significant changes in the status and expression of the mitochondrial (mt) genetic system in bladder smooth muscle immediately after obstruction is initiated. Here we investigate quantitatively the severity of the mt genetic response to partial outlet obstruction in both short-and long-term obstructed rabbits. Based on previous functional studies, bladders with mass < 6 fold greater than control were considered compensated; bladders with mass > 6 fold that of control were considered decompensated. Analyses of DNA from compensated rabbit bladders showed that relative mt genome copy number decreased to 30% of control values. Transcript analyses for these samples showed that mt RNA levels increased 3 fold to compensate for lower template copy number. Analysis of decompensated bladders demonstrated that mt genome copy number increased to approximately 90% of control levels; mt transcripts progressively decreased in these samples by as much as 30 fold. In contrast, transcription of a mt-related nuclear gene decreased 3-9 fold in compensated bladders but increased 10-30 fold in decompensated bladders. Activity for the cytochrome oxidase complex, and for the mt enzyme citrate synthase, decreased steadily with increasing bladder hypertrophy. These data suggest that bladder dysfunction following partial outlet obstruction is mediated partly by a significant loss in mt and mt-related nuclear gene coordination. C1 DEPT VET AFFAIRS MED CTR, MED RES SERV, PHILADELPHIA, PA 19104 USA. MED COLL PENN & HAHNEMANN UNIV, SCH MED, DEPT MICROBIOL & IMMUNOL, PHILADELPHIA, PA 19102 USA. UNIV PENN, SCH MED, DEPT SURG, DIV UROL, PHILADELPHIA, PA 19104 USA. FU PHS HHS [33559, 44689, 26508] NR 28 TC 11 Z9 11 U1 0 U2 0 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0300-8177 EI 1573-4919 J9 MOL CELL BIOCHEM JI Mol. Cell. Biochem. PD AUG PY 1997 VL 173 IS 1-2 BP 95 EP 102 PG 8 WC Cell Biology SC Cell Biology GA XU450 UT WOS:A1997XU45000011 PM 9278259 ER PT J AU Felsenfeld, AJ Rodriguez, M AF Felsenfeld, AJ Rodriguez, M TI Impact of the method of calculation on assessment of the parathyroid hormone calcium set point SO NEPHROLOGY DIALYSIS TRANSPLANTATION LA English DT Letter C1 HOSP REINA SOFIA,UNIT INVEST,CORDOBA,SPAIN. RP Felsenfeld, AJ (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. RI Rodriguez, teresa/H-5452-2011 NR 5 TC 0 Z9 0 U1 0 U2 1 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD, ENGLAND OX2 6DP SN 0931-0509 J9 NEPHROL DIAL TRANSPL JI Nephrol. Dial. Transplant. PD AUG PY 1997 VL 12 IS 8 BP 1768 EP 1769 DI 10.1093/ndt/12.8.1768 PG 2 WC Transplantation; Urology & Nephrology SC Transplantation; Urology & Nephrology GA XQ982 UT WOS:A1997XQ98200051 PM 9269674 ER PT J AU Payami, H Schellenberg, GD Zareparsi, S Kaye, J Sexton, GJ Head, MA Matsuyama, SS Jarvik, LF Miller, B McManus, DQ Bird, TD Katzman, R Heston, L Norman, D Small, GW AF Payami, H Schellenberg, GD Zareparsi, S Kaye, J Sexton, GJ Head, MA Matsuyama, SS Jarvik, LF Miller, B McManus, DQ Bird, TD Katzman, R Heston, L Norman, D Small, GW TI Evidence for association of HLA-A2 allele with onset age of Alzheimer's disease SO NEUROLOGY LA English DT Article ID APOLIPOPROTEIN-E; TYPE-4 ALLELE; GENE; LONGEVITY; LOCUS; MEN; FREQUENCY; EPSILON-4; LINKAGE; MARKER AB Our earlier studies had suggested a possible association between the HLA-A2 allele and Alzheimer's disease (AD). In the present study are tested the hypothesis that A2 is associated with earlier AD onset. We performed two independent studies: a collaborative study with 111 patients and a confirmatory study with 96 patients. We found similar patterns of reduced age at onset as a function of A2 in both data sets. Overall, A2 was associated with a significant S-year shift to earlier onset. The effects of A2 and epsilon 4 on age at onset appeared additive. Our results suggest A2, or a closely linked gene, modulates onset age of AD. Association with A2 would suggest an immune/inflammatory response mechanism for AD. C1 OREGON HLTH SCI UNIV,VET AFFAIRS MED CTR,DEPT NEUROL,PORTLAND,OR 97201. OREGON HLTH SCI UNIV,VET AFFAIRS MED CTR,DEPT PUBL HLTH & PREVENTAT MED SURG & MED,PORTLAND,OR 97201. OREGON HLTH SCI UNIV,VET AFFAIRS MED CTR,DEPT MED TRANSPLANTAT MED,PORTLAND,OR 97201. OREGON HLTH SCI UNIV,VET AFFAIRS MED CTR,LAB IMMUNOGENET & TRANSPLANTAT,PORTLAND,OR 97201. UNIV WASHINGTON,VET AFFAIRS PEUGEOT SOUND HLTH CARE SYST,DIV NEUROL,SEATTLE,WA 98195. UNIV WASHINGTON,VET AFFAIRS PEUGEOT SOUND HLTH CARE SYST,DEPT PSYCHIAT,SEATTLE,WA 98195. VET AFFAIRS MED CTR,SEATTLE,WA 98108. UNIV CALIF SAN DIEGO,DEPT NEUROSCI,SAN DIEGO,CA 92103. SO ILLINOIS UNIV,DEPT NEUROL,SPRINGFIELD,IL. UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024. HARBOR UCLA MED CTR,DEPT NEUROL,LOS ANGELES,CA. RP Payami, H (reprint author), OREGON HLTH SCI UNIV,VET AFFAIRS MED CTR,DEPT MOL & MED GENET,CDW 2,745 SW GAINES RD,PORTLAND,OR 97201, USA. OI Kaye, Jeffrey/0000-0002-9971-3478 FU NIA NIH HHS [U24 AG021886, AG0513C, AG08017, AG10123] NR 35 TC 68 Z9 69 U1 0 U2 2 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0028-3878 J9 NEUROLOGY JI Neurology PD AUG PY 1997 VL 49 IS 2 BP 512 EP 518 PG 7 WC Clinical Neurology SC Neurosciences & Neurology GA XR937 UT WOS:A1997XR93700033 PM 9270587 ER PT J AU Morrow, NS Garrick, T AF Morrow, NS Garrick, T TI Effects of intermittent tail shock or water avoidance on proximal colonic motor contractility in rats SO PHYSIOLOGY & BEHAVIOR LA English DT Article DE proximal colon; tail shock; water avoidance; defecation; bowel dysfunction; feeding ID CORTICOTROPIN-RELEASING FACTOR; IRRITABLE BOWEL SYNDROME; GASTROINTESTINAL TRANSIT; PSYCHOLOGICAL STRESS; RESTRAINT STRESS; EMOTIONAL-STRESS; NEUROPEPTIDE-Y; MOTILITY; CRF; COLD AB Changes in proximal colonic mechanical activity and defecation during exposure to three different types of experimental stressors were examined in rats chronically implanted with 2 force transducers on the proximal colon. To validate the integrity of the recording system, meal-induced changes in proximal colonic contractility were initially measured in all rats 1-2 days prior to stress induction. Different groups of ad lib fed rats were then exposed to tail shock, re-exposure to the shock chamber or water avoidance for 1 h over the next 1-2 days. Two types of phasic colonic contractions, long (0-3/min) and short (6-8/min) duration, were analyzed separately using a computer. Long duration contractions were significantly elevated 21-71% over fasting basal values from 61-120 min following a meal. No other consistent changes during the prandial or postprandial period were observed. Tail shock significantly suppressed proximal colonic contractility from pre-shock values and increased fecal output and fluid content when compared to ad lib fed rats that were not shocked. Fecal output increased but proximal colonic contractility did not change when previously shocked rats were re-exposed to the tail shock chamber but not shocked. In rats exposed to water avoidance, proximal colonic contractility was minimally suppressed but defecation was significantly greater than home cage control animals. These results indicate that proximal colonic contractile activity is differentially altered by exposure to different environmental stressors and may be a contributing factor in stress-induced bowel dysfunction. (C) 1997 Elsevier Science Inc. C1 UNIV CALIF LOS ANGELES,DEPT PSYCHIAT,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,DEPT BIOBEHAV SCI,LOS ANGELES,CA 90024. RP Morrow, NS (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,DIGEST DIS RES CTR,11301 WILSHIRE BLVD,RM 203,LOS ANGELES,CA 90073, USA. FU NIDDK NIH HHS [DK 41301] NR 32 TC 19 Z9 19 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0031-9384 J9 PHYSIOL BEHAV JI Physiol. Behav. PD AUG PY 1997 VL 62 IS 2 BP 233 EP 239 DI 10.1016/S0031-9384(97)00108-X PG 7 WC Psychology, Biological; Behavioral Sciences SC Psychology; Behavioral Sciences GA XN410 UT WOS:A1997XN41000003 PM 9251963 ER PT J AU Carey, K AF Carey, K TI A panel data design for estimation of hospital cost functions SO REVIEW OF ECONOMICS AND STATISTICS LA English DT Article ID MARKET-STRUCTURE; COMPETITION; INDUSTRY; QUALITY AB This paper estimates a multiple-output hospital cost function using a panel data technique that allows for correlation between unobservable individual effects and observable determinants of behavior. Analysis of 1733 facilities for the period of 1987-1991 yields estimates that differ widely from those obtained from a more standard cross-sectional procedure. While the latter method results in negative and stable measures of ray economies of scale, the panel model indicates positive economies of scale that fall slightly over time. RP Carey, K (reprint author), US DEPT VET AFFAIRS,WASHINGTON,DC, USA. NR 38 TC 46 Z9 47 U1 3 U2 6 PU M I T PRESS PI CAMBRIDGE PA FIVE CAMBRIDGE CENTER, CAMBRIDGE, MA 02142 SN 0034-6535 J9 REV ECON STAT JI Rev. Econ. Stat. PD AUG PY 1997 VL 79 IS 3 BP 443 EP 453 DI 10.1162/003465300556850 PG 11 WC Economics; Social Sciences, Mathematical Methods SC Business & Economics; Mathematical Methods In Social Sciences GA XP330 UT WOS:A1997XP33000012 ER PT J AU Pekary, AE Chopra, IJ Berg, L Hershman, JM AF Pekary, AE Chopra, IJ Berg, L Hershman, JM TI Sphingomyelinase and phospholipase A(2) regulate type I deiodinase expression in FRTL-5 cells SO THYROID LA English DT Article ID TUMOR-NECROSIS-FACTOR; RAT-THYROID CELLS; FACTOR-ALPHA; IODOTHYRONINE DEIODINASE; SIGNAL-TRANSDUCTION; INTERFERON-GAMMA; ARACHIDONIC-ACID; CERAMIDE; IDENTIFICATION; INTERLEUKIN-1 AB We have previously reported (Mol. Cell. Endocrinol. (1994) 101, R31-R35) that the proinflammatory cytokines, tumor necrosis factor-alpha (TNF), interleukin-1 beta (IL-1 beta), and interferon-gamma (IFN-gamma), have a marked inhibitory effect on the expression and activity of type I iodothyronine deiodinase (D1) in FRTL-5 rat thyroid cells, while the antiinflammatory cytokine, transforming growth factor-beta(1) (TGF-beta(1)) had no effect. These three proinflammatory cytokines utilize a number of intracellular second messenger systems including the pathways beginning with activation of sphingomyelinase and phospholipase A(2). We have studied the time-dependent and dose-dependent effects of sphingomyelinase, ceramide, phospholipase A(2) (PLA(2)), and arachidonic acid on the expression and activity of D1 in FRTL-5 cells. Sphingomyelinase (0.3 U/mL) inhibited D1 activity 55% and reduced D1 mRNA levels 70% to 90% by 8 hours. Similar treatment with 10 U/mL PLA(2) inhibited D1 activity 54%. Treatment with 15 mu M 5,8,11-eicosatriynoic acid (ETI), a nonmetabolizable analog of arachidonic acid, or 15 mu M ceramide for 3 hours reduced D1 activity with a half-time of disappearance (t(1/2)) of 4.2 hours and 3.7 hours, respectively, but ETI and ceramide did not alter the D1 immunoreactivity or mRNA levels. Treatment for 8 hours with cycloheximide (5 or 10 mu g/mL) had no effect on the D1 mRNA level, but blocked the TNF-induced reduction of this mRNA. We conclude that proinflammatory cytokines inhibit D1 expression and activity in FRTL-5 cells, in part, by activation of sphingomyelinase and PLA(2) that results in (1) competitive inhibition of D1 activity by the enzymatic products ceramide and arachidonic acid and (2) reduction of D1 mRNA stability by protein synthesis-dependent mechanisms. C1 UNIV CALIF LOS ANGELES,DEPT MED,DIV ENDOCRINOL,LOS ANGELES,CA 90024. RP Pekary, AE (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,ENDOCRINOL RES LAB,11301 WILSHIRE BLVD,BLDG 114,ROOM 200,LOS ANGELES,CA 90073, USA. NR 36 TC 10 Z9 10 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 1050-7256 J9 THYROID JI Thyroid PD AUG PY 1997 VL 7 IS 4 BP 647 EP 654 DI 10.1089/thy.1997.7.647 PG 8 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XU747 UT WOS:A1997XU74700020 PM 9292957 ER PT J AU Roper, JM AF Roper, JM TI Trauma and addiction experiences of African American women - Commentary SO WESTERN JOURNAL OF NURSING RESEARCH LA English DT Editorial Material RP Roper, JM (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 SN 0193-9459 J9 WESTERN J NURS RES JI West. J. Nurs. Res. PD AUG PY 1997 VL 19 IS 4 BP 462 EP 463 PG 2 WC Nursing SC Nursing GA XQ149 UT WOS:A1997XQ14900008 ER PT J AU Richardson, A Heydari, AR AF Richardson, A Heydari, AR TI Mammals: Aging and response to stress. SO FASEB JOURNAL LA English DT Meeting Abstract C1 UTHSCSA, Audie L Murphy Div, S Texas Vet Hlth Care Syst, GRECC, San Antonio, TX 78284 USA. UTHSCSA, Dept Physiol, San Antonio, TX 78284 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD JUL 31 PY 1997 VL 11 IS 9 SU S MA 2527 BP A1289 EP A1289 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA ZK302 UT WOS:000073305603014 ER PT J AU Wong, H Yang, D Hill, JS Nikazy, J Schotz, MC AF Wong, H Yang, D Hill, JS Nikazy, J Schotz, MC TI Lipoprotein lipase subunit arrangement SO FASEB JOURNAL LA English DT Meeting Abstract C1 Univ Calif Los Angeles, Los Angeles, CA USA. W Los Angeles Vet Affairs Med Ctr, Los Angeles, CA 90073 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD JUL 31 PY 1997 VL 11 IS 9 SU S MA 139 BP A882 EP A882 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA ZK302 UT WOS:000073305600625 ER PT J AU Glassman, P Peabody, J Model, K AF Glassman, P Peabody, J Model, K TI The illusion of deterministic rules - Response SO ANNALS OF INTERNAL MEDICINE LA English DT Letter C1 NATL ECON RES ASSOCIATES INC,LOS ANGELES,CA 90017. RP Glassman, P (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 1 TC 1 Z9 1 U1 0 U2 0 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD JUL 15 PY 1997 VL 127 IS 2 BP 166 EP 166 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA XK158 UT WOS:A1997XK15800027 ER PT J AU DellItalia, LJ Meng, QC Balcells, E Wei, CC Palmer, R Hageman, GR Durand, J Hankes, GH Oparil, S AF DellItalia, LJ Meng, QC Balcells, E Wei, CC Palmer, R Hageman, GR Durand, J Hankes, GH Oparil, S TI Compartmentalization of angiotensin II generation in the dog heart - Evidence for independent mechanisms in intravascular and interstitial spaces SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE angiotensin-converting enzyme inhibitors; left ventricular function; angiotensins; heart; heart hypertrophy ID CARDIAC-HYPERTROPHY; ADENOSINE-DEAMINASE; FORMING CHYMASE; EXPRESSION; SYSTEM; QUANTITATION; INCREASES; MYOCYTES; PATHWAYS; PEPTIDES AB Angiotensin-converting enzyme inhibitors have beneficial effects that are presumably mediated by decreased angiotensin II (ANG II) production. In this study, we measure for the first time ANG I and ANG II levels in the interstitial fluid (ISF) space of the heart. ISF and aortic plasma ANG I and II levels were obtained at baseline, during intravenous infusion of ANG I (5 mu M, 0.1 ml/min, 60 min), and during ANG I + the angiotensin-converting enzyme inhibitor captopril (cap) (2.5 mM, 0.1 ml/min, 60 min) in six anesthetized open-chested dogs, ISF samples were obtained using microdialysis probes inserted into the left ventricular myocardium (3-4 probes/dog). ANC I increased mean arterial pressure from 102+/-3 (SEM) to 124+/-3 mmHg (P < 0.01); addition of cap decreased MAP to 95+/-3 mmHg (P < 0.01). ANG I infusion increased aortic plasma ANG I and ANG II (pg/ml) (ANG I = 101+/-129 to 370+/-158 pg/ml, P < 0.01; and ANG II = 22+/-40 to 466+/-49, P < 0.01); addition of cap further increased ANG I(1,790+/-158, P < 0.01) and decreased ANG II (33+/-49, P < 0.01), ISF ANG I and ANG II levels (pg/ml) were > 100-fold higher than plasma levels, and did not change from baseline (8,122+/-528 and 6,333+/-677), during ANG 1(8,269+/-502 and 6,139+/-695) or ANG I + cap (8,753+/-502 and 5,884+/-695), The finding of very high ANG I and ANG II levels in the ISF vs, intravascular space that are not affected by IV ANG I or cap suggests that ANG II production and/or degradation in the heart is compartmentalized and mediated by different enzymatic mechanisms in the interstitial and intravascular spaces. C1 UNIV ALABAMA,BIRMINGHAM VET AFFAIRS MED CTR,BIRMINGHAM,AL 35294. UNIV ALABAMA,DEPT MED,DIV CARDIOVASC DIS,BIRMINGHAM,AL 35294. UNIV ALABAMA,DEPT PHYSIOL & BIOPHYS,BIRMINGHAM,AL 35294. AUBURN UNIV,COLL VET MED,AUBURN,AL 36849. FU NHLBI NIH HHS [R01 HL54816, 2T32HLO7457-16A1]; NICHD NIH HHS [HD SCOR HL 17667] NR 37 TC 141 Z9 142 U1 1 U2 3 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD JUL 15 PY 1997 VL 100 IS 2 BP 253 EP 258 DI 10.1172/JCI119529 PG 6 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA XN372 UT WOS:A1997XN37200003 PM 9218500 ER PT J AU Li, SL Valente, AJ Zhao, SJ Clark, RA AF Li, SL Valente, AJ Zhao, SJ Clark, RA TI PU.1 is essential for p47(phox) promoter activity in myeloid cells SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID CHRONIC GRANULOMATOUS-DISEASE; RESPIRATORY BURST OXIDASE; COLONY-STIMULATING FACTOR-1; CCAAT DISPLACEMENT PROTEIN; TRANSCRIPTION FACTOR PU.1; TUMOR-NECROSIS-FACTOR; RNA-POLYMERASE-II; NADPH OXIDASE; CD11B PROMOTER; GENE PROMOTER AB Expression of the phagocyte cytosolic protein p47(phox), a component of NADPH oxidase, is restricted mainly to myeloid cells. To study the cis elements and trans acting factors responsible for its gene expression, we have cloned and characterized the p47(phox) promoter, A predominant transcriptional start site was identified 21 nucleotides upstream of the translation initiation codon, To identify the gene promoter sequences, transient transfections of HL-60 human myeloid cells were performed with a series of 5'-deletion p47(phox)-luciferase reporter constructs that extended as far upstream as -3050 bp relative to the transcriptional start site, The -224 and -86 constructs had the strongest p47(phax) promoter activity, whereas the -46 construct showed a major reduction in activity and the -36 construct a complete loss of activity. DNase I footprint analysis identified a protected region from -57 to -53. This region containing a consensus PU.1 site bound specifically both PU,I present in nuclear extracts from myeloid cells and PU.1 synthesized in vitro. Mutations of this site eliminated PU.1 binding and abolished the ability of the p47(phox) promoter to direct expression of the reporter gene, The p47(phox) promoter was active in all myeloid cell Lines tested (HL-60, THP-1, U937, PLB-985), but not in non-myeloid cells (HeLa, HEK293). Finally, PU.1 transactivated the p47(phox)-luciferase constructs in HeLa cells, We conclude that, similar to certain other myeloid specific genes, p47(phox) promoter activity in myeloid cells requires PU.1. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. S TEXAS VET HLTH CARE SYST,AUDIE L MURPHY DIV,SAN ANTONIO,TX 78284. FU NHLBI NIH HHS [HL52665]; NIAID NIH HHS [R01 AI020866, AI20866, R37 AI020866] NR 70 TC 56 Z9 56 U1 0 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JUL 11 PY 1997 VL 272 IS 28 BP 17802 EP 17809 DI 10.1074/jbc.272.28.17802 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA XK165 UT WOS:A1997XK16500077 PM 9211934 ER PT J AU Pisegna, JR Leyton, J Coelho, T Hida, T Jakowlew, S Birrer, M Fridkin, M Gozes, I Moody, TW AF Pisegna, JR Leyton, J Coelho, T Hida, T Jakowlew, S Birrer, M Fridkin, M Gozes, I Moody, TW TI PACAP hybrid: A new PACAP receptor antagonist SO LIFE SCIENCES LA English DT Article DE PACAP; PACAP antagonist; receptor; splice variants ID CYCLASE-ACTIVATING POLYPEPTIDE; VASOACTIVE INTESTINAL POLYPEPTIDE; LUNG-CANCER CELLS; ADENYLATE-CYCLASE; FUNCTIONAL EXPRESSION; MOLECULAR-CLONING; STRUCTURAL REQUIREMENTS; I RECEPTOR; PEPTIDE; VIP AB The effects of pituitary adenylate cyclase activating polypeptide (PACAP) hybrid, a synthetic antagonist, was investigated on NIH/3T3 cells containing PACAP receptor (R) splice variants (SVs). PACAPhybrid inhibited I-125-PACAP-27 binding to NIH/3T3 cells stably expressing PACAP-R basic, SV- 1, SV-2 or SV-3 with an IC50 of 1000 nM. PACAPhybrid antagonized the ability of PACAP-27 to elevate cAMP regardless of the PACAP-R SV used. PACAP was more efficacious at increasing cytosolic Ca2+ in NIH/3T3 cells containing PACAP-R SV-2 than PACAP-R basic, SV-1 or SV-3. PACAPhybrid antagonized the increase in cytosolic Ca2+ caused by PACAP-27 regardless of the PACAP-R SV used. PACAP was more potent at elevating c-fos mRNA using NIH/3T3 cells transfected with PACAP-R SV-2 than PACAP-R basic, SV-1 or SV-3. PACAPhybrid antagonized the increase in c-fos mRNA caused by PACAP-27. These data suggest that PACAPhybrid is a useful PACAP receptor antagonist for PACAP-R SVs. C1 NCI,BIOMARKERS & PREVENT RES BRANCH,ROCKVILLE,MD 20850. UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,CURE,CTR DIGEST DIS,LOS ANGELES,CA 90073. WEIZMANN INST SCI,DEPT ORGAN CHEM,IL-76100 REHOVOT,ISRAEL. SACKLER SCH MED,DEPT CLIN BIOCHEM,TEL AVIV,ISRAEL. NR 30 TC 15 Z9 15 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0024-3205 J9 LIFE SCI JI Life Sci. PD JUL 3 PY 1997 VL 61 IS 6 BP 631 EP 639 DI 10.1016/S0024-3205(97)00438-4 PG 9 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA XK788 UT WOS:A1997XK78800006 PM 9250719 ER PT J AU Carabello, BA Crawford, FA AF Carabello, BA Crawford, FA TI Valvular heart disease SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Review ID AORTIC-VALVE REPLACEMENT; LEFT-VENTRICULAR FUNCTION; CORONARY-ARTERY DISEASE; CHRONIC MITRAL REGURGITATION; LONG-TERM SURVIVAL; ASYMPTOMATIC PATIENTS; EJECTION PERFORMANCE; SYMPTOMATIC PATIENTS; NATURAL-HISTORY; WALL STRESS C1 MED UNIV S CAROLINA,GAZES CARDIAC RES INST,CHARLESTON,SC 29425. MED UNIV S CAROLINA,DEPT SURG,DIV CARDIOTHORAC SURG,CHARLESTON,SC 29425. RALPH H JOHNSON DEPT VET AFFAIRS,CHARLESTON,SC 29425. RP Carabello, BA (reprint author), MED UNIV S CAROLINA,DIV CARDIOL,DEPT MED,171 ASHLEY AVE,CHARLESTON,SC 29425, USA. NR 99 TC 165 Z9 171 U1 0 U2 3 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD JUL 3 PY 1997 VL 337 IS 1 BP 32 EP 41 DI 10.1056/NEJM199707033370107 PG 10 WC Medicine, General & Internal SC General & Internal Medicine GA XH186 UT WOS:A1997XH18600007 PM 9203430 ER PT J AU Kiraly, A Suto, G Guth, PH Tache, Y AF Kiraly, A Suto, G Guth, PH Tache, Y TI Mechanisms mediating gastric hyperemic and acid responses to central TRH analog at a cytoprotective dose SO AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY LA English DT Article DE prostaglandins; capsaicin; calcitonin gene-related peptide antagonist; gastric mucosal blood flow; vagus ID GENE-RELATED PEPTIDE; THYROTROPIN-RELEASING-HORMONE; MUCOSAL BLOOD-FLOW; HYDROGEN GAS CLEARANCE; VAGAL-STIMULATION; NERVOUS-SYSTEM; RAT; SECRETION; CGRP; NEUROPEPTIDES AB Gastric hyperemic and acid responses to the stable thyrotropin-releasing hormone (TRH) analog RX-77368 injected intracisternally at a cytoprotective dose were investigated, as well as the underlying mechanisms of the responses. Gastric acid secretion (GAS), mucosal blood flow (GMBF; measured by the hydrogen gas clearance technique), and mucosal vascular resistance (GMVR) and mean arterial pressure (MAP) were assessed simultaneously for 30 min before and after RX-77368 (1.5 ng) administration in urethan-anesthetized rats. RX-77368 increased GMBF from 46.8 +/- 5.3 to 100.6 +/- 20.9 ml.min(-1).100 g(-1) and MAP from 70.3 +/- 2.1 to 84.3 +/- 5.9 mmHg and decreased GMVR from 1.50 +/- 0.33 to 0.84 +/- 0.08 mmHg.ml(-1).min.100 g, whereas GAS was not significantly altered (1.8 +/- 0.4 vs. 4.7 +/- 1.7 mu mol/30 min) in vehicle-pretreated rats. The GMBF, MAP, and GMVR responses to RX-77368 were not modified by indomethacin (5 mg/kg ip), whereas GAS was increased. In rats pretreated with capsaicin (125 mg/kg sc) or calcitonin gene-related peptide (CGRP) antagonist hCGRP-(8-37), intracisternal RX-77368 did not increase GMBF or decrease GMVR but did stimulate GAS. These data show that vagal stimulation by the TRH analog RX-77368 injected intracisternally at a nonacid secretory dose increases GMBF. Gastric hyperemia is mediated by CGRP contained in capsaicin-sensitive afferent fibers, whereas acid secretion is under the inhibitory influence of prostaglandins and CGRP. C1 W LOS ANGELES VET AFFAIRS MED CTR, DIGEST DIS RES CTR, CURE, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, DEPT MED, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, BRAIN RES INST, LOS ANGELES, CA 90073 USA. FU NIDDK NIH HHS [DK-30110, DK-41301]; NIMH NIH HHS [MH-00663] NR 36 TC 15 Z9 15 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0193-1857 J9 AM J PHYSIOL-GASTR L JI Am. J. Physiol.-Gastroint. Liver Physiol. PD JUL PY 1997 VL 273 IS 1 BP G31 EP G38 PG 8 WC Gastroenterology & Hepatology; Physiology SC Gastroenterology & Hepatology; Physiology GA XL328 UT WOS:A1997XL32800005 PM 9252506 ER PT J AU Kaltashov, IA Doroshenko, V Cotter, RJ Takayama, K Qureshi, N AF Kaltashov, IA Doroshenko, V Cotter, RJ Takayama, K Qureshi, N TI Confirmation of the structure of lipid A derived from the lipopolysaccharide of Rhodobacter sphaeroides by a combination of MALDI, LSIMS, and tandem mass spectrometry SO ANALYTICAL CHEMISTRY LA English DT Article ID FAST ATOM BOMBARDMENT; RHODOPSEUDOMONAS-SPHAEROIDES; FATTY-ACIDS; ATCC-17023 AB The chemical structure of nontoxic diphosphoryl lipid A from Rhodobacter sphaeroides was confirmed using a combination of LSIMS (on a two-sector mass spectrometer) and MALDI (on time-of-flight and ion trap mass spectrometers) in conjunction with tandem mass spectrometry in both positive and negative ion modes. Accurate molecular weight measurement accompanied by the analysis of fragment ion masses yielded the composition of fatty acyl groups. Tandem experiments (collisionally induced dissociation of both quasimolecular and oxonium ions) were also performed, revealing the precise location and nature of the fatty acyl groups on the disaccharide backbone. C1 JOHNS HOPKINS UNIV,SCH MED,DEPT PHARMACOL,MIDDLE ATLANTIC MASS SPECTROMETRY LAB,BALTIMORE,MD 21205. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MYCOBACTERIOL RES LAB,MADISON,WI 53705. UNIV WISCONSIN,COLL AGR & LIFE SCI,DEPT BACTERIOL,MADISON,WI 53706. FU NCRR NIH HHS [R01RR08912]; NIGMS NIH HHS [GM50870, R01GM33967] NR 16 TC 46 Z9 46 U1 2 U2 6 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0003-2700 J9 ANAL CHEM JI Anal. Chem. PD JUL 1 PY 1997 VL 69 IS 13 BP 2317 EP 2322 DI 10.1021/ac9612943 PG 6 WC Chemistry, Analytical SC Chemistry GA XG497 UT WOS:A1997XG49700019 PM 9212704 ER PT J AU Kharasch, ED Russell, M Garton, K Lentz, G Bowdle, TA Cox, K AF Kharasch, ED Russell, M Garton, K Lentz, G Bowdle, TA Cox, K TI Assessment of cytochrome P450 3A4 activity during the menstrual cycle using alfentanil as a noninvasive probe SO ANESTHESIOLOGY LA English DT Article; Proceedings Paper CT 1996 Annual Meeting of the American-Society-of-Anesthesiologists CY OCT 18-25, 1996 CL NEW ORLEANS, LA SP Amer Soc Anesthesiologists DE anesthetics, intravenous, alfentanil, fentanyl, sufentanil; analgesics, opioid, alfentanil, fentanyl, sufentanil; enzymes, cytochrome P450 3A4; menstrual cycle, pharmacokinetics, variability, women ID ERYTHROMYCIN BREATH TEST; CYCLOSPORINE-A OXIDASE; HUMAN-LIVER; IN-VIVO; HUMAN HEPATOCYTES; PRIMARY CULTURES; RAT-LIVER; PHARMACOKINETICS; METABOLISM; GENDER AB Background: Gender-dependent differences in cytochrome P450 activity, drug metabolism, drug elimination, and their clinical consequences are increasingly apparent. P450 3A4 is the most abundant P450 isoform in the human liver and is responsible for metabolizing a vast and diverse assortment of therapeutic agents, including opioids, benzodiazepines, and local anesthetics. P450 3A4 activity is higher in women, influenced by steroid hormone levels, and is speculated to vary during the menstrual cycle. This investigation tested the hypothesis that P450 3A4 activity varies during the menstrual cycle. Alfentanil clearance was used as a metabolic probe for P450 3A4 activity. Methods: Alfentanil (20 mu g/kg bolus) was administered to nine nonsmoking, nonpregnant female volunteers (age, 26 +/- 5 yr) with normal menstrual cycles on three separate occasions during the same menstrual cycle: days 2 (menstrual phase), 13 (estrogen peak), and 21 (progesterone peak). Venous plasma alfentanil concentrations were determined by gas chromatography-mass spectrometry. Alfentanil clearance was determined by noncompartmental methods and by a three-compartment model with both pooled population and two-stage analysis. Results: There was no significant difference in any measure of alfentanil clearance. Noncompartmental clearances (mean +/- SD) were 3.62 +/- 0.76, 3.81 +/- 0.96, and 3.60 +/- 0.84 ml/kg/ min, respectively, on days 2, 13, and 21 of the menstrual cycle. Conclusions: Alfentanil clearances were not different on menstrual cycle days 2, 13, and 21, strongly suggesting no change in P450 3A4 activity. Menstrual cycle differences in alfentanil clearances do not contribute to interindividual variability in alfentanil disposition In women. If other P450 3A4 substrates are comparable, then menstrual cycle variability in their metabolism may not be a consideration in dosing or in the design of pharmacokinetic investigations. C1 VET AFFAIRS PUGET SOUND HLTH CARE SYST,ANESTHESIOL SERV,SEATTLE,WA. RP Kharasch, ED (reprint author), UNIV WASHINGTON,DEPT ANESTHESIOL,BOX 356540,SEATTLE,WA 98195, USA. FU NCRR NIH HHS [M01 RR00037] NR 54 TC 51 Z9 52 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0003-3022 J9 ANESTHESIOLOGY JI Anesthesiology PD JUL PY 1997 VL 87 IS 1 BP 26 EP 35 DI 10.1097/00000542-199707000-00005 PG 10 WC Anesthesiology SC Anesthesiology GA XJ862 UT WOS:A1997XJ86200009 PM 9232131 ER PT J AU deVirgilio, C Toosie, K Arnell, T Lewis, RJ Donayre, CE Baker, JD Melany, M White, RA AF deVirgilio, C Toosie, K Arnell, T Lewis, RJ Donayre, CE Baker, JD Melany, M White, RA TI Asymptomatic carotid artery stenosis screening in patients with lower extremity atherosclerosis: A prospective study SO ANNALS OF VASCULAR SURGERY LA English DT Article; Proceedings Paper CT 15th Annual Meeting of the Southern-California-Vascular-Surgical-Society CY SEP 15-17, 1996 CL DANA POINT, CA SP So Calif Vasc Surg Soc ID SURGERY; DISEASE AB Eighty-nine male veterans presenting to a vascular surgery clinic with symptomatic lower extremity atherosclerosis were prospectively screened by duplex scan for asymptomatic carotid artery stenosis (GAS). Their chief complaint was: claudication (90%), rest pain (6%), and ischemic ulcer or gangrene (4%). The mean ankle-brachial index (ABI) was 0.77. Twenty-five CAS > 50% were detected in 18 (20%) patients. Twelve CAS > 75% were detected in 11 (12%) patients. There was no difference between patients with and without CAS > 50% with regards to mean ABI, history of: angina, diabetes, hypertension, prior coronary artery bypass, or history of smoking. Carotid bruit was associated with ipsilateral CAS > 50% [p<0.0001, sensitivity (52%), specificity (88%), positive predictive value (41%), negative predictive value (92%)]. As a result of the screening, eight elective carotid endarterectomies have been performed to date in six (7%) patients with one transient twelfth cranial nerve paresis as the only postoperative complication. We conclude that: (1) male patients presenting with symptomatic lower extremity atherosclerosis have a 20% prevalence of asymptomatic CAS > 50%, (2) there is no correlation between the degree of lower extremity ischemia and CAS > 50%, (3) carotid bruit is significantly associated with CAS > 50%, but has a low sensitivity, and (4) routine CAS screening should be considered far all male patients with symptomatic lower extremity atherosclerosis regardless of whether a bruit is present. C1 W LOS ANGELES VET AFFAIRS MED CTR, DEPT SURG, LOS ANGELES, CA 90073 USA. W LOS ANGELES VET AFFAIRS MED CTR, DEPT RADIOL, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, HARBOR MED CTR, DEPT EMERGENCY MED, TORRANCE, CA 90509 USA. NR 10 TC 26 Z9 27 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0890-5096 J9 ANN VASC SURG JI Ann. Vasc. Surg. PD JUL PY 1997 VL 11 IS 4 BP 374 EP 377 DI 10.1007/s100169900063 PG 4 WC Surgery; Peripheral Vascular Disease SC Surgery; Cardiovascular System & Cardiology GA XJ350 UT WOS:A1997XJ35000007 PM 9236993 ER PT J AU Lyon, JG Cariski, D Keisler, L Rosenbek, J Levine, R Kumpula, J Ryff, C Coyne, S Blanc, M AF Lyon, JG Cariski, D Keisler, L Rosenbek, J Levine, R Kumpula, J Ryff, C Coyne, S Blanc, M TI Communication Partners: Enhancing participation in life and communication for adults with aphasia in natural settings SO APHASIOLOGY LA English DT Article AB Communication partners is a novel aphasia treatment plan whose aim is to restore a sense of purpose, direction, and control to daily life for both patient and caregiver. Treatment involves the participation of a community volunteer who, once paired with an aphasic adult, acts as a Liaison in bridging clinical and real-life pursuits. Initially, treatment pairs are taught how to interact freely and effectively. Once comfortable in each other's company these partners embark on activities of the patient's choosing at home or in the community. Results from 10 treated pairs failed to yield significant differences on the Boston Diagnostic Aphasia Examination, Communication Abilities in Daily Living, or the Affect Balance Scale. However, all participants, i.e. patients, caregivers, and communication partners, noted statistically significant gains in the aphasic adult's well-being and communication on two investigator-constructed questionnaires. Other informal measures also supported the use of this treatment. The significance of these findings, as well as methodological considerations when undertaking this type of research, are discussed. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. UNIV WISCONSIN,SCH MED,DEPT NEUROL,MADISON,WI. UNIV WISCONSIN,DEPT PSYCHOL,MADISON,WI 53706. UNIV WISCONSIN,DEPT COMMUN DISORDERS,MADISON,WI 53706. RP Lyon, JG (reprint author), LIVING APHASIA INC,6344 HILLSANDWOOD RD,MAZOMANIE,WI 53560, USA. NR 17 TC 106 Z9 107 U1 0 U2 13 PU TAYLOR & FRANCIS LTD PI LONDON PA ONE GUNPOWDER SQUARE, LONDON, ENGLAND EC4A 3DE SN 0268-7038 J9 APHASIOLOGY JI Aphasiology PD JUL PY 1997 VL 11 IS 7 BP 693 EP 708 DI 10.1080/02687039708249416 PG 16 WC Clinical Neurology SC Neurosciences & Neurology GA XJ639 UT WOS:A1997XJ63900005 ER PT J AU Raskind, MA Sadowsky, CH Sigmund, WR Beitler, PJ Auster, SB AF Raskind, MA Sadowsky, CH Sigmund, WR Beitler, PJ Auster, SB TI Effect of tacrine on language, praxis, and noncognitive behavioral problems in Alzheimer disease SO ARCHIVES OF NEUROLOGY LA English DT Article AB Objective: To examine the effects of tacrine hydrochloride in patients with Alzheimer disease (AD) and detectable baseline deficits in discrete cognitive and noncognitive parameters who were enrolled in a previously reported multicenter, double-blind, 30-week trial. Design: An exploratory analysis using last observation carried forward. The study population included a placebo group (n=181) and all patients randomized to treatment with 160 mg/d of tacrine hydrochloride (n=238), regardless of highest dose achieved or duration of tacrine therapy. Study Population: Male and female subjects, at least 50 years of age, with mild to moderate AD and detectable baseline deficits in discrete cognitive and noncognitive parameters. Main Outcome Measures: Change from baseline to last observation carried forward in discrete subscale scores of the Alzheimer's Disease Assessment Scale (ADAS): cognitive (memory, language, praxis) and noncognitive (mood, behavior). Improvement was defined as a decrease of at least 1 point hom baseline; stabilization was defined as no change or a decrease from baseline. Results: Compared with the placebo group, the percentage of patients receiving tacrine whose conditions improved or stabilized was significantly greater for 8 of 11 ADAS-cognitive items (word recall, word recognition, orientation, language production, comprehension, word finding, following commands, ideational praxis) and for the ADAS-noncognitive items: cooperation, delusions, and pacing. Conclusions: Tacrine stabilizes or improves specific behavioral deficits and symptoms in AD. The previous demonstration of tacrine's effect on global cognitive function has been extended by suggesting an association between tacrine therapy and improvements in individual cognitive and noncognitive items of the ADAS. Effects of tacrine in clinical practice might be more accurately and efficiently assessed by measuring individual ADAS cognitive and noncognitive items relevant to individual patient pretreatment clinical status. C1 UNIV WASHINGTON,SCH MED,SEATTLE,WA. PALM BEACH NEUROL GRP,PALM BEACH,FL. WARNER LAMBERT PARKE DAVIS,PARKE DAVIS PHARMACEUT RES DIV,MORRIS PLAINS,NJ 07950. RP Raskind, MA (reprint author), VET AFFAIRS PUGET SOUND HLTH CARE SYST,MENTAL HLTH SERV 116,1600 S COLUMBIA WAY,SEATTLE,WA 98109, USA. RI Sigmund, Wolfgang/B-2856-2008 NR 9 TC 92 Z9 93 U1 0 U2 1 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-9942 J9 ARCH NEUROL-CHICAGO JI Arch. Neurol. PD JUL PY 1997 VL 54 IS 7 BP 836 EP 840 PG 5 WC Clinical Neurology SC Neurosciences & Neurology GA XL131 UT WOS:A1997XL13100007 PM 9236571 ER PT J AU Mutton, DL Scremin, AME Barstow, TJ Scott, MD Kunkel, CF Cagle, TG AF Mutton, DL Scremin, AME Barstow, TJ Scott, MD Kunkel, CF Cagle, TG TI Physiologic responses during functional electrical stimulation leg cycling and hybrid exercise in spinal cord injured subjects SO ARCHIVES OF PHYSICAL MEDICINE AND REHABILITATION LA English DT Article ID INDIVIDUALS; PARAPLEGIA; DISEASE AB Objectives: (1) To determine if a hybrid exercise (leg plus arm) training program performed immediately after functional electrical stimulation (FES) leg cycle exercise (LCE) training would further improve aerobic capacity when compared with FES leg cycle training alone, and (2) to compare the submaximal responses occurring during both FES-LCE alone and hybrid exercise in the same SCI subjects. Design: Nonrandomized control trial whereby subjects act as their own control. Setting: Outpatient rehabilitation in a primary care hospital, Patients: A volunteer sample (n = 11) of men 20 to 50 years old with complete spinal cord injury, free from cardiovascular and metabolic disease with spasticity. Interventions: Three phases of exercise training: phase I, progressive FES-LCE to 30 minutes of exercise (n = 11); phase II, 35.2 +/- 16.2 sessions of FES-LCE (n = 11); phase III, 41.4 +/- 17.7 30-minute sessions of hybrid exercise in = 8), Main Outcome Measures: (1) Aerobic capacity-a further increase after hybrid exercise when compared with FES-LCE alone; (2) submaximal physiologic parameters (oxygen uptake [(V) over dot O-2], heart rate [HR], blood lactate [BLa-])-measurement of these during constant work rate exercise and a training effect, Results: (V) over dot O-2 (the body's ability to utilize oxygen) significantly improved (p < .05) after both FES-LCE and then further after hybrid training, Hybrid exercise training resulted in significantly (p < .05) greater work rates and (V) over dot O-2 values than both FES-LCE at baseline and training work rates, Conclusion: These subjects demonstrated that hybrid exercise performed twice a week provided sufficient intensity to improve aerobic capacity and provide a medium whereby patients with SCI can burn more calories than via FES-LCE alone. This has important implications for improving the health and fitness levels of individuals with SCI and may ultimately reduce their risk of cardiovascular disease. (C) 1997 by the American Congress of Rehabilitation Medicine and the American Academy of Physical Medicine and Rehabilitation. C1 W LOS ANGELES VET AFFAIRS MED CTR,PHYS MED & REHABIL SERV 117,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90024. HARBOR UCLA MED CTR,DEPT MED,DIV RESP & CRIT CARE PHYSIOL & MED,TORRANCE,CA 90509. UNIV SO CALIF,RANCHO LOS AMIGOS MED CTR,DOWNEY,CA 90242. ALBUQUERQUE VET AFFAIRS MED CTR,PHYS MED & REHABIL SERV,ALBUQUERQUE,NM. UNIV NEW MEXICO,DEPT ORTHOPED,ALBUQUERQUE,NM 87131. NR 25 TC 50 Z9 51 U1 0 U2 10 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0003-9993 J9 ARCH PHYS MED REHAB JI Arch. Phys. Med. Rehabil. PD JUL PY 1997 VL 78 IS 7 BP 712 EP 718 DI 10.1016/S0003-9993(97)90078-2 PG 7 WC Rehabilitation; Sport Sciences SC Rehabilitation; Sport Sciences GA XK216 UT WOS:A1997XK21600006 PM 9228873 ER PT J AU Denning, GM Leidal, KG Holst, VA Iyer, SS Pearson, DW Clark, JR Nauseef, WM Clark, RA AF Denning, GM Leidal, KG Holst, VA Iyer, SS Pearson, DW Clark, JR Nauseef, WM Clark, RA TI Calreticulin biosynthesis and processing in human myeloid cells: Demonstration of signal peptide cleavage and N-glycosylation SO BLOOD LA English DT Article ID CALCIUM-BINDING PROTEIN; ENDOPLASMIC-RETICULUM PROTEINS; MUSCLE SARCOPLASMIC-RETICULUM; RO/SS-A AUTOANTIGEN; HIGH-AFFINITY; GENE-EXPRESSION; MOLECULAR-CLONING; CA2+ BINDING; HL-60 CELLS; RAT-LIVER AB Calreticulin is a soluble endoplasmic reticulum protein comprising the major storage reservoir for inositol trisphosphate-releasable calcium. Although its highly conserved primary structure and a wide range of functions have been well described, less attention has been paid to its biosynthesis, particularly in human tissues. We report analyses of synthesis, proteolytic processing and glycosylation of human calreticulin, In both HL-60 and PLB-985 myeloid cell lines calreticulin was immunoprecipitated as a single 60-kD species without evidence of precursor forms. However, in vitro cell-free synthesis produced a 62-kD primary translation product, which in the presence of microsomal membranes, was processed by cotranslational signal peptide cleavage to a 60-kD species that comigrated with mature calreticulin produced in myeloid cells, Neither tunicamycin treatment of the cells nor endoglycosidase digestion of calreticulin resulted in any forms other than the 60-kD protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, suggesting that the potential site for N-glycosylation at asparagine-327 was unmodified, However, oxidative derivatization of carbohydrate components with digoxigenin showed that human calreticulin produced in either HL-60 cells or Sf9 insect cells is glycosylated, indicating that glycosylated and nonglycosylated human calreticulin have indistinguishable electrophoretic mobilities, Direct measurement by phenol-H2SO4 confirmed the presence of carbohydrate on recombinant human calreticulin, These data show that human myeloid calreticulin undergoes cotranslational signal peptide cleavage and posttranslational N-linked glycosylation, Although glycosylation of calreticulin has been shown in rat liver and bovine liver and brain, it has been reported to be lacking in other tissues including human lymphocytes. (C) 1997 by The American Society of Hematology. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. DEPT VET AFFAIRS MED CTR,DEPT MED,IOWA CITY,IA 52242. UNIV IOWA,IOWA CITY,IA. S TEXAS VET HLTH CARE SYST,AUDIE L MURPHY DIV,DEPT MED,SAN ANTONIO,TX. FU BLRD VA [I01 BX000513] NR 49 TC 29 Z9 30 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD JUL 1 PY 1997 VL 90 IS 1 BP 372 EP 381 PG 10 WC Hematology SC Hematology GA XH428 UT WOS:A1997XH42800043 PM 9207473 ER PT J AU Mendez, MF Cherrier, MM Perryman, KM AF Mendez, MF Cherrier, MM Perryman, KM TI Differences between Alzheimer's disease and vascular dementia on information processing measures SO BRAIN AND COGNITION LA English DT Article ID MULTIPLE LACUNAR INFARCTS; CLINICAL-DIAGNOSIS; REACTION-TIME; LESIONS AB This study evaluated information processing differences between 30 vascular dementia (VaD) patients, 30 Alzheimer's disease (AD) patients, and 30 normal elderly (NE) controls. They were administered a complex reaction time test, a continuous performance test (CPT), and a neuropsychological battery. Compared to NE, both dementia groups had significantly slower motor reaction times and made more errors on the CPT. Compared to AD patients, the VaD patients were slower in stimulus categorization time and had increasing omission errors and persistent commission errors throughout the CPT trial. VaD, which usually includes frontal-subcortical circuit injury, can impair mental speed and stimulus response initiation. (C) 1997 Academic Press. C1 UNIV CALIF LOS ANGELES,DEPT NEUROL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,DEPT BIOBEHAV SCI,LOS ANGELES,CA 90024. RP Mendez, MF (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,NEUROBEHAV UNIT 691 116AF,13001 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 36 TC 37 Z9 38 U1 2 U2 2 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0278-2626 J9 BRAIN COGNITION JI Brain Cogn. PD JUL PY 1997 VL 34 IS 2 BP 301 EP 310 DI 10.1006/brcg.1997.0923 PG 10 WC Neurosciences; Psychology, Experimental SC Neurosciences & Neurology; Psychology GA XH668 UT WOS:A1997XH66800009 PM 9220093 ER PT J AU Brunicardi, FC Moldovan, S Gilman, S Livingston, E Passaro, E AF Brunicardi, FC Moldovan, S Gilman, S Livingston, E Passaro, E TI An Internet multicast symposium concerning the microcirculation of the islets of Langerhans SO CELL TRANSPLANTATION LA English DT Article DE Internet; MBone; pancreatic islet; microcirculation ID CELLULAR PERFUSION; CORROSION CASTS; HUMAN PANCREAS; MONKEY; MICROSCOPY; ORDER AB The Internet Globally-linked Computer System was used to conduct an international scientific symposium, The symposium was held at the VAMC-Long Beach and consisted of prepared lectures that were multicast over the Internet, The basic unit of hardware used for the Internet Multicast was the Silicon Graphics Indy Unix Workstation, which was equipped with a color video camera, The multicast required four additional pieces of software from the file transfer protocol, The multicast backbone protocol allowed for simultaneous audio and video signals (the presenter, the slides, and the videotape images of islet microcirculation studies) to be transmitted over the computer network, The faculty included 12 experts in microcirculation, who gave 15-min lectures that included a question and answer period, All lectures were received at 14 computer stations in six countries, Eleven of the faculty gave their lectures at the VAMC-Long Beach, and one gave her lecture at the Massachusetts Institute of Technology in Boston, MA. The presenter from Boston was able to receive and answer questions from the faculty at the VAMC-Long Beach, An estimated $12,000 was saved in travel, hotel, and food costs and an estimated 180 travel hours were saved by viewers who did not have to travel to the symposium, We have demonstrated that a scientific symposium can be conducted using the Internet, We propose that many of our future meetings will be organized over the computer network, This format of multiimage projections allows us to effectively communicate in a personal way with a reduction in expensive and time-consuming travel. (C) 1997 Elsevier Science Inc. C1 W LOS ANGELES VET AFFAIRS MED CTR,DEPT SURG,LOS ANGELES,CA 90073. VA EDUC CTR,LONG BEACH,CA. NR 24 TC 0 Z9 0 U1 1 U2 4 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0963-6897 J9 CELL TRANSPLANT JI Cell Transplant. PD JUL-AUG PY 1997 VL 6 IS 4 BP 439 EP 442 PG 4 WC Cell & Tissue Engineering; Medicine, Research & Experimental; Transplantation SC Cell Biology; Research & Experimental Medicine; Transplantation GA XN786 UT WOS:A1997XN78600010 PM 9258519 ER PT J AU Singh, BN AF Singh, BN TI Amiodarone: The expanding antiarrhythmic role and how to follow a patient on chronic therapy SO CLINICAL CARDIOLOGY LA English DT Review DE class III antiarrhythmic drugs; ventricular tachycardia and fibrillation; sotalol; atrial fibrillation; heart failure; myocardial infarction; proarrhythmia; monitoring amiodarone therapy ID LOW-DOSE AMIODARONE; VENTRICULAR TACHYARRHYTHMIAS; INTRAVENOUS AMIODARONE; MYOCARDIAL-INFARCTION; ATRIAL-FIBRILLATION; DOUBLE-BLIND; TRIAL; DEFIBRILLATORS; TACHYCARDIA; RECURRENT AB Amiodarone was introduced as an antiarrhythmic compound in the early 1970s and was approved in the U.S. for the treatment of refractory ventricular arrhythmias in late 1984. Since that time the drug has become the most widely studied antiarrhythmic compound with expanding potential indications, including maintaining stability of sinus rhythm, secondary prevention in the survivors of myocardial infarction, and prolongation of survival in certain subsets of patients with congestive heart failure. Intravenous amiodarone was introduced in the U.S. in 1995 for the control of recurrent destabilizing ventricular tachycardia or ventricular fibrillation resistant to conventional therapy. The level of comfort in its use has risen considerably in the recent past. This has stemmed from the reasonably decisive evidence that class I agents increase mortality in patients with structural heart disease. In contrast, amiodarone either reduces mortality or its effect is neutral; this is consistent with its low to negligible proarrhythmic actions. The drug does not aggravate heart failure and it may even increase left ventricular ejection fraction and improve exercise capacity. Above all, it is becoming increasingly evident from wider experience and from controlled clinical trials that the side-effect profile of the drug is not as compelling an issue as it appeared to be when first used in much higher doses. Therefore, the overall objective of amiodarone therapy is to use the lowest dose that produces a defined therapeutic end point without causing serious side effects. Careful clinical surveillance in conjunction with monitoring of certain laboratory parameters and indices of efficacy at regular intervals permits the drug to be used effectively in a large number of patients who fail to respond to, or are intolerant of other antiarrhythmic compounds. Many experienced clinicians have begun to consider the use of amiodarone as first-line therapy in certain disorders of rhythm, especially in patients with severely compromised ventricular function. C1 UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA 90024. RP Singh, BN (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,CARDIOL SECT,11301 WILSHIRE BLVD 111E,LOS ANGELES,CA 90073, USA. NR 31 TC 40 Z9 41 U1 0 U2 1 PU CLINICAL CARDIOLOGY PUBL CO PI MAHWAH PA PO BOX 832, MAHWAH, NJ 07430-0832 SN 0160-9289 J9 CLIN CARDIOL JI Clin. Cardiol. PD JUL PY 1997 VL 20 IS 7 BP 608 EP 618 PG 11 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA XH249 UT WOS:A1997XH24900004 PM 9220176 ER PT J AU Graybill, JR AF Graybill, JR TI Can we agree on the treatment of candidiasis? Response SO CLINICAL INFECTIOUS DISEASES LA English DT Editorial Material ID MULTIPLE-DOSE PHARMACOKINETICS; AMPHOTERICIN-B; ORAL SOLUTION; FLUCONAZOLE; ITRACONAZOLE; FLUCYTOSINE; CANDIDEMIA; MENINGITIS; LEUKEMIA; TRIAL RP Graybill, JR (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. NR 21 TC 17 Z9 17 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD JUL PY 1997 VL 25 IS 1 BP 60 EP 62 DI 10.1086/514503 PG 3 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA XL367 UT WOS:A1997XL36700011 PM 9243033 ER PT J AU Anzueto, A Peters, JI Tobin, MJ DelosSantos, R Seidenfeld, JJ Moore, G Cox, WJ Coalson, JJ AF Anzueto, A Peters, JI Tobin, MJ DelosSantos, R Seidenfeld, JJ Moore, G Cox, WJ Coalson, JJ TI Effects of prolonged controlled mechanical ventilation on diaphragmatic function in healthy adult baboons SO CRITICAL CARE MEDICINE LA English DT Article DE mechanical ventilation; lung pathology/morphology; diaphragmatic function ID LUNG INJURY; PRESSURE; MUSCLES; VOLUME; EDEMA AB Objective: To study diaphragmatic strength and endurance after a prolonged period of mechanical ventilation, Design: Prospective animal study, Setting: Animal research laboratory, Subjects: Seven uninjured adult baboons (Papio cynocephalus) were anesthetized with ketamine, sedated, paralyzed, and mechanically ventilated, Animals were monitored with pulmonary arterial and peripheral arterial catheters, Interventions: Mechanical Ventilation was provided for 11 days with an FIO2 of 0.21 and tidal Volume of 15 mL/kg, Pulmonary function tests, including lung volumes, arterial brood gases, and chest radiographs were also monitored, Nursing care procedures included frequent turning, chest physiotherapy, and endotracheal suction, Antacids and prophylactic antibiotics (intravenous penicillin, topical polymyxin B, and gentamicin sulfate) were administered, In three animals, fishhook electrodes were surgically placed around both phrenic nerves on both day 0 and after 11 days of mechanical ventilation for diaphragmatic stimulation, On day 0, the electrodes were removed after phrenic nerve stimulation studies were performed, After 11 days of mechanical ventilation, animals were electively killed and full autopsy performed. Measurements and Main Results: Hemodynamic and pulmonary function parameters were measured at baseline and every day during the 11 days of mechanical ventilation, Diaphragmatic strength and endurance were measured on days 0 and 11. Diaphragmatic endurance was determined by an inspiratory resistive loading protocol, There were no significant changes in hemodynamics, lung volumes, or gas exchange during the period of mechanical ventilation, On day 7,the chest radiographs showed patchy lobar atelectasis in six animals, which cleared by day 11 in ail but two of the animals, Lung pathology showed mild, focal pneumonitis, By day 11, maximum transdiaphragmatic pressure had decreased by 25% from day 0 and diaphragmatic endurance had decreased by 36%, Conclusions: Eleven days of mechanical ventilation and neuromuscular blockade in healthy baboons resulted in nonsignificant changes in hemodynamics, oxygenation, and/or lung function, However, significant impairment in diaphragmatic endurance and strength were seen, Based on these results, it is likely that prolonged mechanical ventilation by itself impairs diaphragmatic function independent of underlying lung disease. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV PULM DIS CRIT CARE MED,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX 78284. SW FDN BIOMED RES,SAN ANTONIO,TX 78284. UNIV ARKANSAS MED SCI HOSP,LITTLE ROCK,AR 72205. LOYOLA UNIV,STRITCH SCH MED,DEPT MED,DIV PULM & CRIT CARE MED,CHICAGO,IL 60611. VET AFFAIRS EDWARD HINES JR HOSP,MAYWOOD,IL. RP Anzueto, A (reprint author), AUDIE L MURPHY MEM VET HOSP DIV,PULM DIS SECT,S TEXAS VET HLTH CARE SYST,SAN ANTONIO,TX 78284, USA. FU NHLBI NIH HHS [HL-23578] NR 18 TC 94 Z9 99 U1 0 U2 3 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0090-3493 J9 CRIT CARE MED JI Crit. Care Med. PD JUL PY 1997 VL 25 IS 7 BP 1187 EP 1190 DI 10.1097/00003246-199707000-00021 PG 4 WC Critical Care Medicine SC General & Internal Medicine GA XL366 UT WOS:A1997XL36600021 PM 9233746 ER PT J AU Mendez, MF Stanley, TM Medel, NM Li, ZP Tedesco, DT AF Mendez, MF Stanley, TM Medel, NM Li, ZP Tedesco, DT TI The vascular dementia of Fabry's disease SO DEMENTIA AND GERIATRIC COGNITIVE DISORDERS LA English DT Article DE Fabry's disease; dementia; vascular dementia; cerebrovascular disease ID ALPHA-GALACTOSIDASE-A; INVOLVEMENT; GENE AB Fabry's disease, a rare X-linked disorder of glycosphingolipid metabolism, can present as an insidious dementia in middle or later life. This genetic disorder produces a deficiency of a-galactosidase A which results in the deposition of glycosphingolipids in blood vessel walls in the brain as well as in the kidney, heart, peripheral nerves, and other organs. Among the cerebrovascular manifestations of this disorder is a vascular dementia from involvement of multiple small penetrating blood vessels, Fabry's disease is a consideration in the workup of an otherwise unexplained vascular dementia, particularly in males less than 65 years of age. C1 UNIV CALIF LOS ANGELES,DEPT NEUROL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,DEPT PSYCHIAT,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,DEPT PATHOL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,DEPT INTERNAL MED,LOS ANGELES,CA 90024. RP Mendez, MF (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 23 TC 40 Z9 42 U1 0 U2 1 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 1420-8008 J9 DEMENT GERIATR COGN JI Dement. Geriatr. Cogn. Disord. PD JUL-AUG PY 1997 VL 8 IS 4 BP 252 EP 257 DI 10.1159/000106640 PG 6 WC Geriatrics & Gerontology; Clinical Neurology; Psychiatry SC Geriatrics & Gerontology; Neurosciences & Neurology; Psychiatry GA XF934 UT WOS:A1997XF93400009 PM 9213072 ER PT J AU Segu, VB Mahvi, DM Wilson, MA Hale, SJ Warner, TF Meredith, M Shenker, Y AF Segu, VB Mahvi, DM Wilson, MA Hale, SJ Warner, TF Meredith, M Shenker, Y TI Use of In-111 pentetreotide scintigraphy in the diagnosis of a midgut carcinoid causing Cushing's syndrome SO EUROPEAN JOURNAL OF ENDOCRINOLOGY LA English DT Article ID SOMATOSTATIN-RECEPTOR SCINTIGRAPHY; ECTOPIC ACTH SYNDROME; CORTICOTROPIN-RELEASING HORMONE; ENDOCRINE TUMORS; DIFFERENTIAL-DIAGNOSIS; LOCALIZATION; MANAGEMENT; ANALOGS AB A 57-year-old man presented with clinical features of hypercortisolism and was diagnosed with ACTH-dependent Cushing's syndrome. Biochemical testing showed partial suppression of urinary free cortisol with high dose dexamethasone. Initial computed tomography (GT) of the chest and abdomen, and magnetic resonance imaging of the pituitary were negative. In-111 pentetreotide scintigraphy with single photon emission computerized tomography revealed two 'hot' lesions in the abdomen which were then confirmed by subsequent directed thin-slice abdominal CT and small bowel barium study, At surgery, two segments of ileum, adjoining mesentery and lymph nodes were resected. Histopathology was consistent with a malignant carcinoid tumor of the ileum which stained intensely for ACTH. Plasma ACTH, and serum and urinary cortisol normalized postoperatively. To our knowledge, this is the first reported case of ileal carcinoid tumor causing Cushing's syndrome with premortem diagnosis. Another unique feature of this case is that In-111 pentetreotide scan provided the decisive clue to localization of the tumor. C1 UNIV WISCONSIN HOSP & CLIN, DEPT MED, SECT ENDOCRINOL DIABET & METAB, CTR CLIN SCI H4568, MADISON, WI 53792 USA. UNIV WISCONSIN HOSP & CLIN, DEPT SURG, MADISON, WI 53792 USA. UNIV WISCONSIN HOSP & CLIN, DEPT RADIOL, MADISON, WI 53792 USA. UNIV WISCONSIN HOSP & CLIN, DEPT PATHOL, MADISON, WI 53792 USA. WILLIAM S MIDDLETON MEM VET ADM MED CTR, MED SERV, MADISON, WI 53705 USA. NR 33 TC 7 Z9 7 U1 0 U2 1 PU SCANDINAVIAN UNIVERSITY PRESS PI OSLO PA PO BOX 2959 TOYEN, JOURNAL DIVISION CUSTOMER SERVICE, N-0608 OSLO, NORWAY SN 0804-4643 J9 EUR J ENDOCRINOL JI Eur. J. Endocrinol. PD JUL PY 1997 VL 137 IS 1 BP 79 EP 83 DI 10.1530/eje.0.1370079 PG 5 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XK498 UT WOS:A1997XK49800016 PM 9242206 ER PT J AU Shea, JA Asch, DA Johnson, RF Staroscik, RN Malet, PF Pollack, BJ Clarke, JR Green, PE Schwartz, JS Williams, SV AF Shea, JA Asch, DA Johnson, RF Staroscik, RN Malet, PF Pollack, BJ Clarke, JR Green, PE Schwartz, JS Williams, SV TI What predicts gastroenterologists' and surgeons' diagnosis and management of common bile duct stones? SO GASTROINTESTINAL ENDOSCOPY LA English DT Article ID ENDOSCOPIC RETROGRADE CHOLANGIOGRAPHY; LAPAROSCOPIC CHOLECYSTECTOMY; OPERATIVE CHOLANGIOGRAPHY; INTRAOPERATIVE CHOLANGIOGRAPHY; MULTIVARIATE-ANALYSIS; RANDOMIZED TRIAL; ROUTINE; EXPERIENCE; PHYSICIANS; INJURIES AB Background: Because the literature suggests numerous indicators of common bile duct stones, we undertook a systematic assessment of physicians' judgments of the clinical utility of eight indicators: patient age, history of jaundice, history of pancreatitis, levels of serum alanine aminotransferase, alkaline phosphatase, amylase, and total bilirubin, and common bile duct diameter on ultrasonography. Methods: Random samples of 1500 gastroenterologists and 1500 surgeons were sent a survey asking them to indicate the importance of each potential indicator of common bile duct stones, the likelihood of common bile duct stones for each of nine clinical vignettes, and whether they would order a preoperative ERCP. An abbreviated survey was sent to nonrespondents. Results: Although there was substantial variation in the importances assigned to each indicator, the most important indicators were serum total bilirubin and diameter of common bile duct on ultrasound. The best predictors of the decision to order an ERCP were perceived likelihood of stones and specialty. The average threshold for ordering an ERCP was 37%. Respondents did not differ from nonrespondents in the perceived importance of the eight indicators. Conclusions: The substantial variation among gastroenterologists and surgeons regarding the optimal approach to common bile duct stones has clinical implications. Patients will receive varying recommendations for care, depending on whom they see. C1 UNIV PENN,WHARTON SCH,DEPT SURG,DEPT MED,DIV GASTROENTEROL,PHILADELPHIA,PA 19104. UNIV PENN,LEONARD DAVIS INST HLTH ECON,PHILADELPHIA,PA 19104. UNIV PENN,SCH MED,CTR CLIN EPIDEMIOL & BIOSTAT,PHILADELPHIA,PA 19104. VET AFFAIRS MED CTR,PHILADELPHIA,PA. ALLEGHENY UNIV HLTH SCI,DEPT SURG,PHILADELPHIA,PA 19102. RP Shea, JA (reprint author), UNIV PENN,WHARTON SCH,DEPT SURG,DEPT MED,DIV GEN INTERNAL MED,RALSTON HOUSE 318,3615 CHESTNUT ST,PHILADELPHIA,PA 19104, USA. FU AHRQ HHS [HS06481] NR 45 TC 12 Z9 12 U1 0 U2 1 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0016-5107 J9 GASTROINTEST ENDOSC JI Gastrointest. Endosc. PD JUL PY 1997 VL 46 IS 1 BP 40 EP 47 DI 10.1016/S0016-5107(97)70208-6 PG 8 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA XQ654 UT WOS:A1997XQ65400008 PM 9260704 ER PT J AU Burack, JH Back, AL Pearlman, RA AF Burack, JH Back, AL Pearlman, RA TI Provoking nonepileptic seizures: The ethics of deceptive diagnostic testing SO HASTINGS CENTER REPORT LA English DT Article ID PSYCHOGENIC SEIZURES; PSEUDOSEIZURES; EPILEPSY; PROVOCATION; SUGGESTION C1 UNIV CALIF BERKELEY,SCH PUBL HLTH,BERKELEY,CA 94720. UNIV WASHINGTON,SCH MED,DIV ONCOL,SEATTLE,WA 98195. UNIV WASHINGTON,SCH MED,DIV GERONTOL & GERIATR MED,SEATTLE,WA 98195. VET AFFAIRS PUGET SOUND HLTH CARE SYST,CTR CLIN,SEATTLE,WA. RP Burack, JH (reprint author), UNIV CALIF SAN FRANCISCO,DIV GEN INTERNAL MED,SAN FRANCISCO,CA 94143, USA. NR 25 TC 5 Z9 5 U1 1 U2 2 PU HASTINGS CENTER PI BRIARCLIFF MANOR PA 255 ELM ROAD, BRIARCLIFF MANOR, NY 10510 SN 0093-0334 J9 HASTINGS CENT REP JI Hastings Cent. Rep. PD JUL-AUG PY 1997 VL 27 IS 4 BP 24 EP 33 DI 10.2307/3528776 PG 10 WC Ethics; Health Care Sciences & Services; Medical Ethics; Social Sciences, Biomedical SC Social Sciences - Other Topics; Health Care Sciences & Services; Medical Ethics; Biomedical Social Sciences GA XR919 UT WOS:A1997XR91900009 PM 9271719 ER PT J AU Williams, DM Grubbs, BG Pack, E Kelly, K Rank, RG AF Williams, DM Grubbs, BG Pack, E Kelly, K Rank, RG TI Humoral and cellular immunity in secondary infection due to murine Chlamydia trachomatis SO INFECTION AND IMMUNITY LA English DT Article ID DEFICIENT MICE; T-CELLS; MOUSE PNEUMONITIS; GAMMA-INTERFERON; HOST DEFENSE; GENITAL-INFECTION; ROLE INVIVO; AGENT; RESOLUTION; ANTIBODY AB A murine model of pneumonia due to the mouse pneumonitis agent (MoPn [murine Chlamydia trachomatis]) in mice deficient in CD4(+) T-cell function (major histocompatibility complex [MHC] class II function [class II-/-], CD8(+) T-cell function (beta(2)-microglobulin deficient, MHC class I deficient [Beta(2)m(-/-)]), B-cell function (C57BL/10J-Igh(tm1Cgn) [Igh(-/-)]), and gamma Interferon (IFN-gamma) (C57BL/6-Ifg(tm1) [Ifg(-/-)]) or interleukin-4 (C57/BLtm1Cgn129 [IL4(-/-)]) production was employed to determine if each of these mechanisms was critical to resistance against reinfection by C. trachomatis or if alternate compensatory mechanisms existed in their absence which could potentially be exploited in vaccine development. Resistance to reinfection with MoPn was heavily dependent on CD4(+) T cells. CD4 T-cell-deficient MHC class II-/- mice sere very susceptible to reinfection with MoPn, showing the critical importance of this cell to resistance. These mice lacked antibody production hut did produce IFN-gamma, apparently hy mechanisms involving NK and CD8(+) T cells. Neutralization of IFN-gamma in these mice led to a borderline increase in susceptibility showing a possible role (albeit small) of this cytokine in this setting, Tumor necrosis factor alpha (TNF-alpha) was also present at increased levels in these mice, Igh(-/-) B-cell-deficient mice which produce no antibody to MoPn were only modestly more susceptible to reinfection than immunized B-cell-intact controls, shelving that antibody, including lung immunoglobulin A, if not an absolute requirement for relatively successful host defense in this setting. Levels of lung IFN-gamma and TNF-alpha were elevated in Igh(-/-) miff compared to those in controls. IL-4(-/-) mice (deficient in Th2 function) could develop normal resistance to reinfection with MoPn. Conversely, normal mice rendered partially IFN-gamma deficient by antibody depletion were somewhat impair ed in their ability to develop acquired immunity to MoPn, again indicating a role for this cytokine in host defense against rechallenge. Of most importance, however, congenitally IFN-gamma-deficient Ifg(-/-) mice (which have elevated levels of other cytokines, including TNF-alpha and granulocyte-macrophage colony-stimulating fatter) are paradoxically more resistant to MoPn rechallenge than controls, showing that IFN-gamma if not an absolute requirement for acquired resistance and implying the presence of very effective compensatory host defense mechanism(s). In vivo depletion of TNF-alpha significantly increased MoPn levels in the lungs in these mice. Thus, resistance to reinfection in this model is flexible and multifactorial and is heavily dependent on CD4(+) T cells, with a probable role for IFN-gamma and TNF-alpha and a possible modest role for Th1-dependent antibody Since IFN-gamma was dispensable in host defense, the highly effective mechanism or mechanisms which can compensate for its absence (which include TNF-alpha) deserve further study. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. UNIV ARKANSAS MED SCI HOSP,DEPT MICROBIOL & IMMUNOL,LITTLE ROCK,AR 72205. RP Williams, DM (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,DIV INFECT DIS,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. FU NIAID NIH HHS [R01 AI026328] NR 35 TC 62 Z9 63 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD JUL PY 1997 VL 65 IS 7 BP 2876 EP 2882 PG 7 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA XF631 UT WOS:A1997XF63100051 PM 9199462 ER PT J AU Mohammad, AR Bauer, RL Yeh, CK AF Mohammad, AR Bauer, RL Yeh, CK TI Spinal bone density and tooth loss in a cohort of postmenopausal women SO INTERNATIONAL JOURNAL OF PROSTHODONTICS LA English DT Article ID OSTEOPOROSIS; PERIODONTITIS AB Osteoporosis has been suggested to contribute to the edentulous condition. However, studies specifically designed to examine the relationship between tooth loss and osteoporosis are scarce, and reported results have been mixed. This study investigated the correlation between tooth loss and spinal bone density in a nonHispanic white female population. Forty-four subjects were recruited and divided into high and low spinal bone density groups based on whether the bone density was higher or lower than the age-adjusted population mean bone density. Missing teeth and periodontal status in these groups were assessed and analyzed. Tooth loss was not significantly different between subjects with low spinal bone density (4.90 +/- 0.89 teeth) and high spinal bone density (3.81 +/- 0.90 teeth). Tooth loss did not differ after age and periodontal adjustment. However, the loss of attachment levels (relative to the cementoenamel junction) was greater in the low spinal bone density subjects (3.42 +/- 0.30 mm) than in the high spinal bone density subjects (2.37 +/- 0.26 mm) (P<.05). The difference may have resulted from gingival recession (P<.05), because the gingival pocket depths were not significantly different between the two groups. In this study, total tooth loss was not directly associated with systemic bone density. However, periodontal disease was negatively associated with spinal bone density. Whether periodontal disease will ultimately lead to greater tooth loss in law spinal bone density group merits further investigation. C1 UNIV SO CALIF,SCH DENT,DEPT DENT MED & PUBL HLTH,LOS ANGELES,CA 90089. UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM HOSP DIV,S TEXAS VET HLTH CARE SYST,CTR GERIATR RES EDUC & CLIN,SAN ANTONIO,TX. UNIV TEXAS,HLTH SCI CTR,DEPT DENT DIAGNOST SCI,SAN ANTONIO,TX. NR 22 TC 41 Z9 43 U1 0 U2 1 PU QUINTESSENCE PUBL CO INC PI CAROL STREAM PA 551 KIMBERLY DR, CAROL STREAM, IL 60188-1881 SN 0893-2174 J9 INT J PROSTHODONT JI Int. J. Prosthodont. PD JUL-AUG PY 1997 VL 10 IS 4 BP 381 EP 385 PG 5 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA XN376 UT WOS:A1997XN37600011 PM 9484049 ER PT J AU Silva, JA Ferrari, MM Leong, GB Weinstock, R AF Silva, JA Ferrari, MM Leong, GB Weinstock, R TI The role of mania in the genesis of dangerous delusional misidentification. SO JOURNAL OF FORENSIC SCIENCES LA English DT Article DE forensic science; forensic psychiatry; dangerousness; delusional misidentification; violence; aggression; mania; bipolar disorder; schizoaffective disorder ID CAPGRAS SYNDROME; SELF; INTERMETAMORPHOSIS; VIOLENCE AB The delusional misidentification syndromes are characterized by a misidentification delusion of the self and/or others. Delusional misidentification syndromes frequently occur in psychotic disorders such as schizophrenia, psychotic disorder due to general medical condition, or psychotic disorder not otherwise specified. On rare occasions these syndromes may be associated with manic states secondary to bipolar disorder, schizoaffective disorder, or general medical conditions. The delusional misidentification syndromes may also be associated with verbal and physical aggression. in this article we present three cases of dangerous delusional misidentification associated with mania. We will discuss the role that mania may have in the genesis of dangerous delusional misidentification. C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. OHIO STATE UNIV,COLL MED,COLUMBUS,OH 43210. VET AFFAIRS OUTPATIENT CLIN,COLUMBUS,OH. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. RP Silva, JA (reprint author), S TEXAS VET HLTH CARE SYST,PSYCHIAT SERV 116A,PSYCHIAT RES UNIT,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. NR 39 TC 3 Z9 3 U1 1 U2 1 PU AMER SOC TESTING MATERIALS PI W CONSHOHOCKEN PA 100 BARR HARBOR DR, W CONSHOHOCKEN, PA 19428-2959 SN 0022-1198 J9 J FORENSIC SCI JI J. Forensic Sci. PD JUL PY 1997 VL 42 IS 4 BP 670 EP 674 PG 5 WC Medicine, Legal SC Legal Medicine GA XL348 UT WOS:A1997XL34800013 PM 9243830 ER PT J AU Agostini, HT Ryschkewitsch, CF Mory, R Singer, EJ Stoner, GL AF Agostini, HT Ryschkewitsch, CF Mory, R Singer, EJ Stoner, GL TI JC virus (JCV) genotypes in brain tissue from patients with progressive multifocal leukoencephalopathy (PML) and in urine from controls without PML: Increased frequency of JCV type 2 in PML SO JOURNAL OF INFECTIOUS DISEASES LA English DT Article; Proceedings Paper CT 48th Annual Meeting of the American-Academy-of-Neurology CY MAR 23-30, 1996 CL SAN FRANCISCO, CA SP Amer Acad Neurol ID HUMAN-IMMUNODEFICIENCY-VIRUS; POLYMERASE CHAIN-REACTION; PROMOTER ENHANCER; BK-VIRUS; DNA; AIDS; REARRANGEMENTS; AMPLIFICATION; INDIVIDUALS; INFECTION AB Progressive multifocal leukoencephalopathy (PML) is caused by the human polyomavirus JC (JCV), and there are at least 4 different genotypes of JCV in the United States, Type 1 strains are of European origin, whereas type 2 and 3 strains are of Asian and African origin, respectively, JCV type 4 strains are derived from a type 1/3 recombinant, In this study, the genotype distribution of JCV strains found in brain tissue or cerebrospinal fluid of 50 PML patients was compared with JCV genotypes excreted in the urine of 103 control subjects, Type determination was based on the polymerase chain reaction-amplified partial sequence of the VP1 coding gene and the noncoding region left of ori, Brain tissues from patients with PML were infected with a significantly higher proportion of JCV type 2 strains than were urine samples from the control group (P = .004), This evidence indicates a biologic difference between JCV genotypes and suggests a difference in their potential to cause PML. C1 NINCDS,EXPT NEUROPATHOL LAB,NIH,BETHESDA,MD 20892. W LOS ANGELES VET AFFAIRS MED CTR,NEUROL SERV,LOS ANGELES,CA 90073. NR 37 TC 56 Z9 56 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0022-1899 J9 J INFECT DIS JI J. Infect. Dis. PD JUL PY 1997 VL 176 IS 1 BP 1 EP 8 DI 10.1086/514010 PG 8 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA XH275 UT WOS:A1997XH27500001 PM 9207343 ER PT J AU Moss, P Charmley, P Mulvihill, E Ziegler, S Raugi, GJ Kern, D Piepkorn, M Gelinas, R AF Moss, P Charmley, P Mulvihill, E Ziegler, S Raugi, GJ Kern, D Piepkorn, M Gelinas, R TI The repertoire of T cell antigen receptor beta-chain variable regions associated with psoriasis vulgaris SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Article DE T lymphocytes; psoriasis; RT-PCR; repertoire; analysis ID LYMPHOCYTES-T; GENE USAGE; LESIONS; KERATINOCYTE; EXPRESSION; GUTTATE; IMMUNE; LINES; SKIN AB We investigated whether the pattern of T-cell receptors expressed by T cells in inflamed psoriatic skin differed substantially from the pattern seen in T cells from the peripheral blood. A bias or restriction in the repertoire of T-cell receptors found in the lesional skin of different patients might imply that specific subsets of T cells were causally associated with initiating or maintaining the lesions, By using a polymerase chain reaction-based assay of T-cell receptor beta-chain variable region mRNA, we found that the patterns of beta-chain mRNAs displayed in 14 samples of lesional skin or six samples of noninvolved skin were not significantly less diverse than the patterns found in matched peripheral blood samples. There was no evidence that the active lesions of multiple patients showed overexpression of T cells expressing one or a few T-cell receptor forms. The pattern of T-cell receptors displayed in clinically normal skin from normal control individuals showed about the same diversity as normal blood. While these results may not exclude either classical antigen or superantigen-based T-cell activation mechanisms in active plaques, the absence of a simple pattern of V beta usage in different patients suggests than other aspects of T-cell biology including trafficking, proliferation, co-stimulation, or responses to cytokines must also be considered. C1 DARWIN MOL CORP,DEPT MOL BIOL & IMMUNOL,BOTHELL,WA 98021. DARWIN MOL CORP,DEPT GEN,BOTHELL,WA 98021. VA PUGET SOUND HLTH CARE SYST,MED SERV,DERMATOL SECT,SEATTLE,WA. UNIV WASHINGTON,SCH MED,DEPT MED,DIV DERMATOL,SEATTLE,WA 98195. NR 34 TC 23 Z9 23 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD JUL PY 1997 VL 109 IS 1 BP 14 EP 19 DI 10.1111/1523-1747.ep12276338 PG 6 WC Dermatology SC Dermatology GA XF815 UT WOS:A1997XF81500003 PM 9204948 ER PT J AU Graybill, JR Bocanegra, R Lambros, C Luther, MF AF Graybill, JR Bocanegra, R Lambros, C Luther, MF TI Granulocyte colony stimulating factor therapy of experimental cryptococcal meningitis SO JOURNAL OF MEDICAL AND VETERINARY MYCOLOGY LA English DT Article DE cryptococcal meningitis; GCSF ID AMPHOTERICIN-B; MURINE CRYPTOCOCCOSIS; NEOFORMANS; MICE; PROTECTION; ANTIBODIES; AIDS AB Cryptococcal meningitis was induced in mice using intracerebral injection of Cryptococcus neoformans. Beginning either 3 days before or 1 day after infection, mice were treated with human recombinant granulocyte colony stimulating factor (hGCSF). In high doses hGCSF reduced the brain tissue burden of C. neoformans but had no effect on survival. The effect of hGCSF was dependent on size of the infecting dose and time of administration. A large innocula of C. neoformans, or when hGCSF was initiated after infection, there was no added benefit. Some groups of mice also received low doses of fluconazole beginning 1 day after infection. Fluconazole both prolonged survival and reduced brain tissue counts of C. neoformans. Combined cytokine/fluconazole therapy was superior to either agent given alone. These studies suggest that hGCSF can add to the efficacy of fluconazole therapy in murine cryptococcosis, and suggest that polymorphonuclear leucocytes contribute to host defence in cryptococcal meningitis. The relative potency of fluconazole appears greater than hGCSF. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. NIAID,BETHESDA,MD 20892. RP Graybill, JR (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,DEPT MED,DIV INFECT DIS,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. FU PHS HHS [N0I-A1-25141] NR 17 TC 28 Z9 28 U1 0 U2 0 PU BLACKWELL SCIENCE LTD PI OXFORD PA P O BOX 88, OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0NE SN 0268-1218 J9 J MED VET MYCOL JI J. Med. Vet. Mycol. PD JUL-AUG PY 1997 VL 35 IS 4 BP 243 EP 247 PG 5 WC Mycology SC Mycology GA XW007 UT WOS:A1997XW00700003 PM 9292420 ER PT J AU Novoa, LJ Nagra, RM Nakawatase, T EdwardsLee, T Tourtellotte, WW Cornford, ME AF Novoa, LJ Nagra, RM Nakawatase, T EdwardsLee, T Tourtellotte, WW Cornford, ME TI Fulminant demyelinating encephalomyelitis associated with productive HHV-6 infection in an immunocompetent adult SO JOURNAL OF MEDICAL VIROLOGY LA English DT Article DE human herpes virus 6; encephalomyelitis; demyelination ID HUMAN HERPESVIRUS-6 INFECTION; EXANTHEM-SUBITUM; ENCEPHALITIS; VIRUS AB Human herpesvirus 6 (HHV-6), the etiologic agent of roseola in young children, has been reported to be detectable in the brain of many neurologically normal adults, although regional localization to plaques of multiple sclerosis has also been demonstrated. Large amounts of this virus were present in multifocal demyelinating white matter lesions of fulminant encephalomyelitis with seizures in a 21-year-old woman with normal immune parameters. Brain biopsy after 3 weeks of neurologic deterioration revealed a viral etiology by light and electron microscopy; the virus was identified as HHV-6 by immunohistochemistry and by polymerase chain reaction (PCR) amplification in biopsy and autopsy specimens. (C) 1997 Wiley-Liss, Inc. C1 UNIV CALIF LOS ANGELES,LOS ANGELES CTY HARBOR MED CTR,DEPT PATHOL,TORRANCE,CA 90509. UNIV CALIF LOS ANGELES,LOS ANGELES CTY HARBOR MED CTR,DEPT NEUROL,TORRANCE,CA 90509. UNIV CALIF LOS ANGELES,SCH MED,BRAIN RES INST,NEUROL RES SERV,W LOS ANGELES VET ADM MED CTR,LOS ANGELES,CA 90024. NR 16 TC 63 Z9 65 U1 1 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0146-6615 J9 J MED VIROL JI J. Med. Virol. PD JUL PY 1997 VL 52 IS 3 BP 301 EP 308 DI 10.1002/(SICI)1096-9071(199707)52:3<301::AID-JMV11>3.0.CO;2-L PG 8 WC Virology SC Virology GA XG228 UT WOS:A1997XG22800011 PM 9210040 ER PT J AU Levine, RL Turski, PA Turnipseed, WD Dulli, DA Grist, TM AF Levine, RL Turski, PA Turnipseed, WD Dulli, DA Grist, TM TI Vasodilatory responses and magnetic resonance angiography - Extracranial and intracranial intravascular flow data SO JOURNAL OF NEUROIMAGING LA English DT Article ID CAROTID-ARTERY STENOSIS; CEREBRAL BLOOD-FLOW; CEREBROVASCULAR REACTIVITY; PERFUSION RESERVE; OCCLUSION; DISEASE; ISCHEMIA; VASOCAPACITANCE; VASOREACTIVITY; ACETAZOLAMIDE AB This study measured the responses of both extracranial (internal carotid arteries) and intracranial (middle cerebral/angular, basilar arteries) intravascular arterial volume flow rates to acetazolamide using phase-contrast magnetic resonance angiography. Twenty-eight newly studied patients were subdivided into four groups: Group l-Nonocclusive, asymptomatic (n = 7, or 14 carotid and middle cerebral/angular artery sides); Group II-unilateral carotid transient ischemic attacks, nonhemodynamic (embolic), varying stenoses (n = 11); Group Ill-unilateral carotid transient ischemic attacks, hemodynamic, varying stenoses (n = 5); and Group lV-unilateral carotid occlusion, asymptomatic (n = 5). The data were separated into nonischemic and ischemic sides so as to illustrate group differences based on vasodilatory responses to acetazolamide. For example, the percent change in volume flow rates over baseline values for the ischemic-side middle cerebral arteries of Group III was significantly the lowest of all of the vasodilatory responses (-25 +/- 11% vs 40 +/- 14% for group II ischemic middle cerebral/angular artery sides, p = 0.008). Group III patients also had significantly lower standing blood pressures (p = 0.012), higher number of transient ischemic attacks (p = 0.008), and shorter duration of events (p = 0.013). Determinations of volume flow rate continue to assist in determining the degree of hemodynamic compromise of a particular vascular territory. C1 UNIV WISCONSIN,DEPT RADIOL,MADISON,WI 53705. UNIV WISCONSIN,DEPT SURG,MADISON,WI 53705. UNIV WISCONSIN,DEPT NEUROL,MADISON,WI 53705. RP Levine, RL (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,DEPT NEUROL,2500 OVERLOOK TERRACE,MADISON,WI 53705, USA. NR 41 TC 5 Z9 5 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 1051-2284 J9 J NEUROIMAGING JI J. Neuroimaging PD JUL PY 1997 VL 7 IS 3 BP 152 EP 158 PG 7 WC Clinical Neurology; Neuroimaging; Radiology, Nuclear Medicine & Medical Imaging SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging GA XM018 UT WOS:A1997XM01800003 PM 9237434 ER PT J AU Saxon, AJ McGuffin, R Walker, RD AF Saxon, AJ McGuffin, R Walker, RD TI An open trial of transdermal nicotine replacement therapy for smoking cessation among alcohol- and drug-dependent inpatients SO JOURNAL OF SUBSTANCE ABUSE TREATMENT LA English DT Article DE nicotine dependence; alcohol dependence; drug dependence; transdermal nicotine; smoking cessation ID FAGERSTROM TOLERANCE QUESTIONNAIRE; TOBACCO WITHDRAWAL; QUITTERS AB An open trial of transdermal nicotine replacement for smoking cessation was conducted Over a 7-month period, all patients admitted to the inpatient alcohol and drug treatment unit of the Seattle Veterans Affairs Medical Center (n = 207) were offered the opportunity to participate in an open trial of transdermal nicotine replacement for smoking cessation. Forty-nine (23.7%) elected to attempt cessation with transdermal nicotine during their inpatient treatment episodes. These subjects received no psychosocial treatments directed specifically at smoking cessation. They smoked a mean of 28.5 (SD = 16.4) cigarettes per day and obtained a mean score of 8.3 (SD = 1.9) on the Fagerstrom Test for Nicotine Dependence. Subjects remained on transdermal nicotine an average of 18.8 (SD = 8.2) days with desire to resume smoking the major reason for discontinuation. Seven subjects (14.3%) self-reported tobacco abstinence at 21 days, and 5 (10.2%) self-reported abstinence as outpatients at 6 weeks. These results show that a substantial proportion of alcohol-and drug-dependent patients entering inpatient treatment are willing to attempt alcohol and illicit drug cessation and tobacco cessation simultaneously and that transdermal nicotine holds promise as a treatment modality in this population. RP Saxon, AJ (reprint author), UNIV WASHINGTON,SCH MED,DEPT PSYCHIAT & BEHAV SCI,VA PUGET SOUND HLTH CARE SYST,SEATTLE,WA 98195, USA. NR 20 TC 36 Z9 36 U1 0 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0740-5472 J9 J SUBST ABUSE TREAT JI J. Subst. Abus. Treat. PD JUL-AUG PY 1997 VL 14 IS 4 BP 333 EP 337 DI 10.1016/S0740-5472(97)00031-7 PG 5 WC Psychology, Clinical; Substance Abuse SC Psychology; Substance Abuse GA YE196 UT WOS:A1997YE19600003 PM 9368209 ER PT J AU Rosenfeld, KE Pearlman, RA AF Rosenfeld, KE Pearlman, RA TI Allocating medical resources: Recommendations for a professional response SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Editorial Material ID RACIAL-DIFFERENCES; UNITED-STATES; APPROPRIATENESS; SYSTEM C1 VET ADM MED CTR,SEATTLE,WA. RP Rosenfeld, KE (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 19 TC 2 Z9 2 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD JUL PY 1997 VL 45 IS 7 BP 886 EP 888 PG 3 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA XH933 UT WOS:A1997XH93300022 PM 9215345 ER PT J AU Royall, DR AF Royall, DR TI Some ''depressive'' symptoms may not imply depression SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Letter ID ALZHEIMERS-DISEASE; DEMENTIA; CORTEX C1 AUDIE L MURPHY MEM VET ADM MED CTR,CTR GERIATR RES EDUC & CLIN,SAN ANTONIO,TX 78284. RP Royall, DR (reprint author), S TEXAS VET HLTH CARE SYST,SAN ANTONIO,TX, USA. NR 15 TC 9 Z9 9 U1 1 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD JUL PY 1997 VL 45 IS 7 BP 891 EP 892 PG 2 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA XH933 UT WOS:A1997XH93300024 PM 9215347 ER PT J AU GuerraGarcia, H AF GuerraGarcia, H TI Charles Bonnet Syndrome and early dementia SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Letter RP GuerraGarcia, H (reprint author), BAYLOR COLL MED,HUFFINGTON CTR AGING,HOUSTON VET AFFAIRS MED CTR,HOUSTON,TX 77030, USA. NR 4 TC 5 Z9 5 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD JUL PY 1997 VL 45 IS 7 BP 893 EP 894 PG 2 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA XH933 UT WOS:A1997XH93300027 PM 9215349 ER PT J AU Walker, RP Kazuko, E Gopalsami, C Bassali, J Lawrence, AM Paloyan, E AF Walker, RP Kazuko, E Gopalsami, C Bassali, J Lawrence, AM Paloyan, E TI Hyperparathyroidism associated with a chronic hypothyroid state SO LARYNGOSCOPE LA English DT Article; Proceedings Paper CT Meeting of the Middle-Section of the American-Laryngological-Rhinological-and-Otological-Society CY JAN 20-21, 1996 CL DEARBORN, MI SP Amer Laryngol Rhinol & Otol Soc Inc ID HASHIMOTOS THYROIDITIS; PARATHYROID ADENOMA; RADIATION; DISEASE; HYPERCALCEMIA; PREVALENCE; NEOPLASIA; SERUM AB Reports of the coexistence of hyperparathyroidism and thyroid disease have raised the issue of a possible etiologic relationship. The present study tests the hypothesis that chronic elevation of thyroid-stimulating hormone (TSH) is related to the development of hyperparathyroidism. Four groups of 60 female rats were treated as follows: group 1, control; group 2, propylthiouracil (PTU) 0.0025%; group 3, PTU 0.0025% plus thyroxine, 5 mu g two times per week; and group 4, only thyroxine. The animals' serum calcium, phosphorus, TSH, thyroxine, and parathyroid hormone (PTH) levels were evaluated at 0, 6, 12, and 18 months. Significant elevation of TSH was sustained throughout the 18 months in groups 2 and 3. The PTH levels were also significantly elevated in both group 2 and group 3 animals (P = 0.02), The histopathologic features of the parathyroids were evaluated at 18 months. In the group 2 (PTU only) animals, which had profound hypothyroid, 44% developed parathyroid adenomas. In the group 3 (PTU plus thyroxine) animals, who had mildly elevated TSH levels, 53% developed parathyroid adenomas. These findings are consistent with the hypothesis that prolonged TSH stimulation may lead to hyperparathyroidism in the rat model. C1 LOYOLA UNIV, MED CTR, DEPT BIOCHEM, MAYWOOD, IL 60153 USA. US DEPT VET AFFAIRS, VET AFFAIRS EDWARD HINES JR HOSP, RES SERV, HINES, IL 60141 USA. US DEPT VET AFFAIRS, VET AFFAIRS EDWARD HINES JR HOSP, EDUC SERV, HINES, IL 60141 USA. HINSDALE HOSP, HINSDALE, IL USA. RP Walker, RP (reprint author), LOYOLA UNIV, MED CTR, DEPT OTOLARYNGOL, 2160 S 1ST AVE, MAYWOOD, IL 60153 USA. NR 40 TC 3 Z9 3 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0023-852X EI 1531-4995 J9 LARYNGOSCOPE JI Laryngoscope PD JUL PY 1997 VL 107 IS 7 BP 903 EP 909 DI 10.1097/00005537-199707000-00013 PG 7 WC Medicine, Research & Experimental; Otorhinolaryngology SC Research & Experimental Medicine; Otorhinolaryngology GA XJ298 UT WOS:A1997XJ29800013 ER PT J AU Klingenspor, M Bodnar, J Xia, YR Welch, C Lusis, AJ Reue, K AF Klingenspor, M Bodnar, J Xia, YR Welch, C Lusis, AJ Reue, K TI Localization of profilin-1 (Pfn1) and a related sequence (Pfn1-rs) to mouse Chromosomes 11 and 15 respectively SO MAMMALIAN GENOME LA English DT Article ID CDNA C1 W LOS ANGELES VET AFFAIRS MED CTR,LIPID RES LAB,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,INST MOL BIOL,LOS ANGELES,CA 90095. UNIV CALIF LOS ANGELES,DEPT PATHOL,LOS ANGELES,CA 90095. UNIV CALIF LOS ANGELES,DEPT MICROBIOL & MOL GENET,LOS ANGELES,CA 90095. UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90095. RI Klingenspor, Martin/D-6930-2011 OI Klingenspor, Martin/0000-0002-4502-6664 FU NHLBI NIH HHS [HL28481] NR 10 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0938-8990 J9 MAMM GENOME JI Mamm. Genome PD JUL PY 1997 VL 8 IS 7 BP 539 EP 540 DI 10.1007/s003359900496 PG 2 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA XG180 UT WOS:A1997XG18000021 PM 9196007 ER PT J AU Mendez, MF Cherrier, MM Cymerman, JS AF Mendez, MF Cherrier, MM Cymerman, JS TI Hemispatial neglect on visual search tasks in Alzheimer's disease SO NEUROPSYCHIATRY NEUROPSYCHOLOGY AND BEHAVIORAL NEUROLOGY LA English DT Article DE Alzheimer's disease; neglect; attention; visuospatial ID UNILATERAL NEGLECT; DIRECTED ATTENTION; RIGHT-HEMISPHERE; LINE BISECTION; ASYMMETRIES; IMPAIRMENT; MOVEMENTS; DEMENTIA; CUES AB Abnormal visual attention may underlie certain visuospatial difficulties in patients with Alzheimer's disease (AD). These patients have hypometabolism and neuropathology in parietal cortex. Given the role of parietal function for visuospatial attention, patients with AD may have relative hemispatial neglect masked by other cognitive disturbances. Fifteen patients with mild-to-moderate AD and 15 healthy elderly controls matched for age, sex, and education were compared on four measures of neglect: the visual search of a complex picture, a letter cancellation task, the Schenkenberg line bisection test, and a computerized line bisection task. Compared with controls, the group with AD was significantly impaired overall in attending to left hemispace on both picture search (F[1,56] = 11.27, p < 0.05) and cancellation tasks (F [1,112] = 12.68, p < 0.01); however, a subgroup of patients with AD had disproportionate difficulty in attending to right hemispace. The performance of the groups did not differ on either of the line bisection tasks regardless of the hand used. In AD, hemispatial neglect on visual search tasks may relate to difficulty in disengaging attention or in visual exploration, as well as to the severity of the disease. Future investigations may implicate neglect in visually related deficits in AD, for example, the prominent difficulty with left turns on driving a car. C1 UNIV CALIF LOS ANGELES,DEPT NEUROL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024. RP Mendez, MF (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,NEUROBEHAV UNIT 691 116AF,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 43 TC 33 Z9 33 U1 1 U2 4 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0894-878X J9 NEUROPSY NEUROPSY BE JI Neuropsychiatr. Neuropsychol. Behav. Neurol. PD JUL PY 1997 VL 10 IS 3 BP 203 EP 208 PG 6 WC Clinical Neurology; Psychiatry; Psychology SC Neurosciences & Neurology; Psychiatry; Psychology GA XU990 UT WOS:A1997XU99000007 PM 9297714 ER PT J AU Low, PA Yao, JK Kishi, Y Tritschler, HJ Schmelzer, JD Zollman, PJ Nickander, KK AF Low, PA Yao, JK Kishi, Y Tritschler, HJ Schmelzer, JD Zollman, PJ Nickander, KK TI Peripheral nerve energy metabolism in experimental diabetic neuropathy SO NEUROSCIENCE RESEARCH COMMUNICATIONS LA English DT Article; Proceedings Paper CT 4th International Symposium on Diabetic Neuropathy and Neurodiab VII CY JUL 15-19, 1997 CL NOORDWIJKERHOUT, NETHERLANDS DE diabetic neuropathy; glucose uptake; energy metabolism ID GLUCOSE-TRANSPORTER PROTEINS; ISCHEMIC CONDUCTION FAILURE; BLOOD-FLOW; THIOCTIC ACID; PERINEURIAL CELLS; INSULIN-TREATMENT; SKELETAL-MUSCLE; OXYGEN DELIVERY; MESSENGER-RNA; RAT AB Peripheral nerve has low energy demands at rest and when maximally stimulated. Coupled with the relatively high energy substrates available, it is more resistant to ischemia and anoxia than brain. Diabetic peripheral nerve, by virtue of its enhanced energy stores, and chronic hypoxia, has an increased resistance to ischemic conduction failure, and subsists proportionately more than tissues such as brain on anaerobic metabolism. Since anaerobic metabolism is very inefficient, however, a reduction in energy substrates can increase the risk of fiber degeneration. Glucose uptake into peripheral neural tissues (sciatic; L5 dorsal root ganglion; superior cervical ganglion) are markedly reduced. alpha-lipoic acid dose- dependently improves glucose uptake. This increase in glucose uptake increases energy stores without a reduction in nerve myoinositol. C1 VA PITTSBURGH HLTH CARE SYST,PATHOL & LAB MED SERV 113A,PITTSBURGH,PA. ASTA MED,FRANKFURT,GERMANY. RP Low, PA (reprint author), MAYO CLIN & MAYO FDN,DEPT NEUROL,200 1ST ST SW,ROCHESTER,MN 55905, USA. NR 60 TC 16 Z9 16 U1 0 U2 3 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD SN 0893-6609 J9 NEUROSCI RES COMMUN JI Neurosci. Res. Commun. PD JUL-AUG PY 1997 VL 21 IS 1 BP 49 EP 56 PG 8 WC Neurosciences SC Neurosciences & Neurology GA XL978 UT WOS:A1997XL97800007 ER PT J AU Wittenkeller, JL Storer, B Bittner, G Schiller, JH AF Wittenkeller, JL Storer, B Bittner, G Schiller, JH TI Comparison of spontaneous and induced mutation rates in an immortalized human bronchial epithelial cell line and its tumorigenic derivative SO ONCOLOGY LA English DT Article DE transformation; mutation; fluctuation analysis; hypoxanthine phosphoribosyltransferase; bronchial epithelial cells, human ID METASTATIC VARIANTS; MUTAGENESIS; INSTABILITY; FIBROBLASTS; TUMOR AB To determine the relationship between neoplastic transformation and increased genetic instability, spontaneous and induced mutation rates were compared in a nontumorigenic, immortalized human bronchial epithelial cell line (NL20) and a tumorigenic cell line (NL20T) spontaneously derived from the NL20 line. Using the hypoxanthine phosphoribosyltransferase (HPRT) locus as a marker for determining mutation rate, fluctuation analysis was utilized to evaluate the spontaneous mutation rate. Induced mutation rates were determined for each cell line after N-methyl-N'-nitro-N-nitrosoguanidine exposure. Both the spontaneous and induced mutation rates were noted to be significantly higher in the nontumorigenic NL20 cell line. These findings suggest that increasing genetic instability, as measured by spontaneous or induced mutation rate in the HPRT locus, does not correlate with tumorigenicity in these cells. C1 UNIV WISCONSIN,CTR COMPREHENS CANC,CLIN SCI CTR K4 666,MADISON,WI 53792. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. NR 21 TC 10 Z9 10 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0030-2414 J9 ONCOLOGY JI Oncology PD JUL-AUG PY 1997 VL 54 IS 4 BP 335 EP 341 PG 7 WC Oncology SC Oncology GA XH064 UT WOS:A1997XH06400013 PM 9216860 ER PT J AU Schwartz, MW Seeley, RJ Woods, SC AF Schwartz, MW Seeley, RJ Woods, SC TI Wasting illness as a disorder of body weight regulation SO PROCEEDINGS OF THE NUTRITION SOCIETY LA English DT Article; Proceedings Paper CT Symposium on New Perspectives in the Anorexia-Cachexia Syndrome CY DEC 03-05, 1996 CL BLACKPOOL, ENGLAND SP Nutr Soc, Clin Nutr & Metab Grp, British Assoc Parenteral & Enteral Nutr ID CORTICOTROPIN-RELEASING FACTOR; CENTRAL-NERVOUS-SYSTEM; NEUROPEPTIDE-Y; OBESE GENE; ENERGY-BALANCE; EXPRESSION; INSULIN; PROTEIN; RATS; HYPERPHAGIA C1 UNIV WASHINGTON,HARBORVIEW MED CTR,DEPT PSYCHOL,SEATTLE,WA 98108. VA PUGET SOUND HLTH CARE SYST,SEATTLE,WA 98108. RP Schwartz, MW (reprint author), UNIV WASHINGTON,HARBORVIEW MED CTR,DEPT MED,SEATTLE,WA 98108, USA. RI Schwartz, Michael/H-9950-2012 FU NIDDK NIH HHS [DK-12829]; NINDS NIH HHS [NS32273] NR 45 TC 8 Z9 8 U1 0 U2 1 PU C A B INTERNATIONAL PI WALLINGFORD PA C/O PUBLISHING DIVISION, WALLINGFORD, OXON, ENGLAND OX10 8DE SN 0029-6651 J9 P NUTR SOC JI Proc. Nutr. Soc. PD JUL PY 1997 VL 56 IS 2 BP 785 EP 791 DI 10.1079/PNS19970079 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA XR144 UT WOS:A1997XR14400025 PM 9264129 ER PT J AU Tyan, ML AF Tyan, ML TI Modulation of the antibody response to sheep red blood cells in normal and immunodeficient XID mice by myo-inositol SO PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE LA English DT Article ID X-LINKED AGAMMAGLOBULINEMIA; PROTEIN-TYROSINE KINASES; CBA-N MICE; VITAMIN-A; IMMUNE DEFECT; B-CELLS; TRISPHOSPHATE AB Over the dose ranges tested, dietary vitamin A and myo-inositol induce similar changes in murine fetal growth and development. Because vitamin A is also known to affect the immune response, studies were conducted to determine if dietary myo-inositol might have an effect on antibody production. It was found that in vivo in inbred mice myo-inositol (4 mg/g of diet) accelerated the rate of appearance of plaque-forming cells (PFC) in a primary response to sheep red blood cells (SRBC), In vitro, myo-inositol accelerated the rate of appearance of colonies of anti-SRBC PFC (foci) and significantly increased the number of PFC per colony, but did not affect the number of foci per culture noted at the end of the culture period. myo-inositol had no effect on the PFC IgM:IgG ratio following a single exposure to the agent, but exposure to myo-inositol in vivo and In vitro resulted in a decrease in the number of IgM PFC per focus in a primary response and IgM and IgG PFC per focus in a secondary response, Based on studies suggesting that myo-inositol or a phosphorylated metabolite might act downstream from Bruton's tyrosine kinase (Btk) in a signal transduction pathway in B cells, immunodeficient CBA/CaHN-XID/J mice were fed a standard diet or the same diet supplemented with 0.4% myo-inositol. Mice given the supplemented diet produced significantly more IgM anti-SRBC antibody than did XID mice given the control diet (4.3 +/- 2.5 vs 1.7 +/- 2.8, 1/log(2)), and produced approximately the same amount as immunocompetent controls (2.9 +/- 0.9). When rechallenged with SRBC, XID mice given supplemental inositol produced significantly more IgM antibody than did the XID and immunocompetent controls (3.6 +/- 0.5 vs 1.8 +/- 1.1 and 1.5 +/- 0.7, respectively). Added dietary inositol did not have a significant effect on primary or secondary IgG responses to SRBC, which remained impaired. These results suggest that dietary myo-inositol or a derivative may be able to modulate B-cell IgM responses by interacting within the inositol second messenger system downstream from Bruton's tyrosine kinase. RP Tyan, ML (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 21 TC 2 Z9 2 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 SN 0037-9727 J9 P SOC EXP BIOL MED JI Proc. Soc. Exp. Biol. Med. PD JUL PY 1997 VL 215 IS 3 BP 258 EP 263 PG 6 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA XF600 UT WOS:A1997XF60000007 PM 9207861 ER PT J AU Deyo, SN Lytton, WW AF Deyo, SN Lytton, WW TI Inhibition can disrupt hypersynchrony in model neuronal networks SO PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY LA English DT Review DE computer models; epilepsy; inhibition; oscillations ID THALAMIC NEURONS; HIPPOCAMPUS; SYNCHRONIZATION; INTERNEURONS; CONDUCTANCES; MODULATION AB 1. Model neuronal network simulations were performed using a reduced Traub neuronal network model. In the absence of inhibition the network produced synchronous population bursting. 2. Bursting of individual neurons was dependent on 1 or more of the following: build-up of charge in the dendritic compartment, prolonged current flow through simulated NMDA associated channels, current flow through T channels. Interburst interval duration and consequent burst frequency was dependent on the density of slow afterhyperpolarizing potassium channels. 3. Addition of an inhibitory interneuron population projecting to GABA(A) receptors resulted in rapid desynchronization of the population, generally after only 1-2 cycles. This effect was found to be due to reduced participation in the individual population burst and to the need for multi-synaptic activation of the individual neuron in the presence of inhibition. 4. This desynchronizing effect could be offset by increasing the strength of interburst hyperpolarization either through increased density of I-AHP, Or through the addition of a separate inhibitory interneuron pool projected to GABA(B) receptors. 5. These data suggest that the synchronizing effects of inhibition may vary depending on circumstances - with desynchronization being dominant in cases characterized by large population bursts such as those seen in epilepsy. C1 UNIV WISCONSIN,WILLIAM S MIDDLETON MEM VET HOSP,DEPT NEUROL,MADISON,WI 53706. NR 28 TC 2 Z9 2 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0278-5846 J9 PROG NEURO-PSYCHOPH JI Prog. Neuro-Psychopharmacol. Biol. Psychiatry PD JUL PY 1997 VL 21 IS 5 BP 735 EP 750 DI 10.1016/S0278-5846(97)00076-6 PG 16 WC Clinical Neurology; Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA XP391 UT WOS:A1997XP39100001 PM 9278947 ER PT J AU Goodfriend, TL Egan, BM AF Goodfriend, TL Egan, BM TI Nonesterified fatty acids in the pathogenesis of hypertension: theory and evidence SO PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS LA English DT Article ID PROTEIN-KINASE-C; BLOOD-PRESSURE; ALDOSTERONE SECRETION; INSULIN STIMULATION; ARTERIAL-PRESSURE; ANGIOTENSIN-II; RAT ADIPOCYTES; FISH-OIL; MECHANISM; INHIBITION AB This paper approaches the hypothesis that fatty acids contribute to hypertension by examining possible interactions of nonesterified fatty acids with renal pressure-natriuresis, peripheral vascular resistance, and the central nervous barostat, three loci where long-term regulation of blood pressure is probably controlled. By inhibiting aldosterone secretion, nonesterified fatty acids may lower blood pressure by facilitating pressure-natriuresis. Oxygenated metabolites of fatty acids appear to stimulate aldosterone secretion. In different experimental situations, fatty acids either constrict or dilate arteries. There is no evidence of an effect of fatty acids on the central nervous barostat, but they do sensitize peripheral vessels to alpha-adrenergic stimuli. Obesity and diabetes are marked by increased incidence of hypertension, and elevated levels of fatty acids or their P450 oxygenated metabolites may contribute to this association. Drugs that influence plasma fatty acids, like heparin, do not have reproducible effects on blood pressure. Experimental evidence suggests but does not prove that nonesterified fatty acids can affect the longterm set-point of blood pressure. C1 UNIV WISCONSIN,DEPT MED,MADISON,WI 53706. UNIV WISCONSIN,DEPT PHARMACOL,MADISON,WI 53706. MED UNIV S CAROLINA,DEPT PHARMACOL,DIV CLIN PHARMACOL,CHARLESTON,SC 29425. MED UNIV S CAROLINA,DEPT MED,DIV CLIN PHARMACOL,CHARLESTON,SC 29425. RP Goodfriend, TL (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,RES SERV,2500 OVERLOOK TERRACE,MADISON,WI 53705, USA. FU NCRR NIH HHS [RR-01070]; PHS HHS [R01-43164] NR 43 TC 13 Z9 13 U1 0 U2 0 PU CHURCHILL LIVINGSTONE PI EDINBURGH PA JOURNAL PRODUCTION DEPT, ROBERT STEVENSON HOUSE, 1-3 BAXTERS PLACE, LEITH WALK, EDINBURGH EH1 3AF, MIDLOTHIAN, SCOTLAND SN 0952-3278 J9 PROSTAG LEUKOTR ESS JI Prostaglandins Leukot. Essent. Fatty Acids PD JUL PY 1997 VL 57 IS 1 BP 57 EP 63 DI 10.1016/S0952-3278(97)90493-2 PG 7 WC Biochemistry & Molecular Biology; Cell Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Cell Biology; Endocrinology & Metabolism GA XM424 UT WOS:A1997XM42400010 PM 9250609 ER PT J AU Tsuang, JW Ho, AP Eckman, TA Shaner, A AF Tsuang, JW Ho, AP Eckman, TA Shaner, A TI Dual diagnosis treatment for patients with schizophrenia who are substance dependent SO PSYCHIATRIC SERVICES LA English DT Editorial Material ID REVOLVING-DOOR; DISORDERS C1 UNIV CALIF LOS ANGELES,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024. RP Tsuang, JW (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,11301 WILSHIRE BLVD 00MH,LOS ANGELES,CA 90073, USA. NR 9 TC 7 Z9 7 U1 1 U2 2 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 1075-2730 J9 PSYCHIATR SERV JI Psychiatr. Serv. PD JUL PY 1997 VL 48 IS 7 BP 887 EP 889 PG 3 WC Health Policy & Services; Public, Environmental & Occupational Health; Psychiatry SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Psychiatry GA XH532 UT WOS:A1997XH53200006 PM 9219296 ER PT J AU Gutierrez, O Melo, M Segura, AM Angel, A Genta, RM Graham, DY AF Gutierrez, O Melo, M Segura, AM Angel, A Genta, RM Graham, DY TI Cure of Helicobacter pylori infection improves gastric acid secretion in patients with corpus gastritis SO SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY LA English DT Article DE acid secretion; antimicrobial therapy; clinical trial; Helicobacter pylori ID DUODENAL-ULCER DISEASE; NECROSIS-FACTOR-ALPHA; GROWTH-FACTOR-ALPHA; CAMPYLOBACTER-PYLORI; ANTRAL GASTRITIS; GENE-EXPRESSION; PARIETAL-CELLS; NITRIC-OXIDE; ERADICATION; INHIBITION AB Background: For more than 30 years it has been known that gastric acid secretion is inversely related to the extent and severity of corpal gastritis. We therefore evaluated the effect of cure of Helicobacter pylori infection on basal and pentagastrin-stimulated acid secretion. Methods: Basal acid output (BAG) and maximal acid output (MAO) were assessed in 11 H. pylori-infected dyspeptic patients (8 women and 3 men; mean age, 28 years) before and after successful anti-H. pylori therapy. Results: The gastritis index was significantly lower after therapy and was associated with an increase in both BAO and MAO after cure of the H. pylori infection (BAG from 0.3 mmol/h to 3.1 mmol/h and MAO from 4.8 mmol/h to 19 mmol/h). Basal and stimulated acid concentrations also increased (29.1 +/- 36.6 to 54 +/- 31 mmol/l and 72.5 +/- 46 to 120.1 +/- 30 mmol/l, respectively, for basal and stimulated acid concentrations; P < 0.05 for peak and MAO, P = 0.07 for BAG). Conclusion: Gastric acid secretion increased into the normal range after successful treatment of H. pylori infection, suggesting that gastric function can recover to normal or almost normal after cure of H. pylori infection. C1 VET AFFAIRS MED CTR 111D,DEPT PATHOL,HOUSTON,TX 77030. VET AFFAIRS MED CTR,DEPT MED,HOUSTON,TX 77030. UNIV NACL COLOMBIA,HOSP SAN JUAN DIOS,DEPT GASTROENTEROL,BOGOTA,COLOMBIA. BAYLOR COLL MED,HOUSTON,TX 77030. NR 53 TC 110 Z9 111 U1 0 U2 0 PU SCANDINAVIAN UNIVERSITY PRESS PI OSLO PA PO BOX 2959 TOYEN, JOURNAL DIVISION CUSTOMER SERVICE, N-0608 OSLO, NORWAY SN 0036-5521 J9 SCAND J GASTROENTERO JI Scand. J. Gastroenterol. PD JUL PY 1997 VL 32 IS 7 BP 664 EP 668 DI 10.3109/00365529708996515 PG 5 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA XJ694 UT WOS:A1997XJ69400008 PM 9246705 ER PT J AU Meining, A Stolte, M Hatz, R Lehn, N Miehlke, S Morgner, A Bayerdorffer, E AF Meining, A Stolte, M Hatz, R Lehn, N Miehlke, S Morgner, A Bayerdorffer, E TI Differing degree and distribution of gastritis in Helicobacter pylori-associated diseases SO VIRCHOWS ARCHIV-AN INTERNATIONAL JOURNAL OF PATHOLOGY LA English DT Article DE gastritis; Helicobacter pylori; peptic ulcer; gastric cancer; MALT lymphoma ID LYMPHOID-TISSUE TYPE; GASTRODUODENAL DISEASE; INTESTINAL METAPLASIA; PEPTIC-ULCERATION; DUODENAL-ULCER; INFECTION; CANCER; RECOGNITION; ERADICATION; REGRESSION AB Infection with Helicobacter pylori (H. pylori) causes gastritis, and may be associated with gastric and duodenal ulcers and also with such malignant diseases as MALT lymphoma and gastric carcinoma. In order to determine whether there are differences in the degree and distribution of gastritis, each patient with H. pylori gastritis only (n = 50) was matched for sex and age with four patients, one each with H. pylori-associated duodenal ulcer, gastric ulcer, gastric carcinoma or MALT lymphoma. From each patient, two biopsies were taken from the antrum and two from the corpus for histopathological examination of H. pylori gastritis. The median summed gastritis score decreases in the following order: antrum: gastric ulcer > duodenal ulcer > gastritis alone > carcinoma > MALT lymphoma, and corpus: gastric ulcer > carcinoma > MALT lymphoma > gastritis alone and duodenal ulcer We conclude that the degree and distribution of H. pylori gastritis differs significantly among H. pylori-associated diseases. These differences may explain some of the underlying pathomechanisms associated with H. pylori infection. C1 KLINIKUM BAYREUTH,INST PATHOL,D-95445 BAYREUTH,GERMANY. UNIV MUNICH,KLINIKUM GROSSHADERN,DEPT SURG,D-8000 MUNICH,GERMANY. TECH UNIV MUNICH,KLINIKUM RECHTS ISAR,DEPT MICROBIOL,D-8000 MUNICH,GERMANY. BAYLOR COLL MED,VA MED CTR,HOUSTON,TX 77030. UNIV MAGDEBURG,DEPT GASTROENTEROL HEPATOL & INFECT DIS,D-39106 MAGDEBURG,GERMANY. UNIV MUNICH,KLINIKUM GROSSHADERN,DEPT MED 2,D-8000 MUNICH,GERMANY. NR 30 TC 68 Z9 70 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0945-6317 J9 VIRCHOWS ARCH JI Virchows Arch. Int. J. Pathol. PD JUL PY 1997 VL 431 IS 1 BP 11 EP 15 DI 10.1007/s004280050063 PG 5 WC Pathology SC Pathology GA XL502 UT WOS:A1997XL50200002 PM 9247628 ER PT J AU Bardin, T Schumacher, HR AF Bardin, T Schumacher, HR TI Special issue - Proceedings of the Symposium Bone Disease for the Rheumatologist: Osteoporosis, New Management Strategies, and Beyond - Held in Philadelphia on September 19-21, 1996 - Under the Aegis of the Societe Francaise de Rhumatologie, the American College of Rheumatology and the French Institute of the University of Pennsylvania - Foreword SO REVUE DU RHUMATISME LA English DT Editorial Material C1 UNIV PENN,VET AFFAIRS MED CTR,SCH MED,ARTHRIT IMMUNOL CTR,PHILADELPHIA,PA 19104. RP Bardin, T (reprint author), UNIV PARIS 07,HOP LARIBOISIERE,RHUMATOL CLIN,CTR VIGGO PETERSEN,PARIS,FRANCE. NR 0 TC 0 Z9 0 U1 0 U2 0 PU EXPANSION SCI FRANCAISE PI PARIS PA 31 BLVD LATOUR MAUBOURG, 75007 PARIS, FRANCE SN 1169-8446 J9 REV RHUM JI Rev. Rhum. PD JUN 30 PY 1997 VL 64 IS 6 SU S BP S1 EP S1 PG 1 WC Rheumatology SC Rheumatology GA XP527 UT WOS:A1997XP52700001 ER PT J AU Jacoby, RF Schlack, S Sekhon, G Laxova, R AF Jacoby, RF Schlack, S Sekhon, G Laxova, R TI Del(10)(q22.3q24.1) associated with juvenile polyposis SO AMERICAN JOURNAL OF MEDICAL GENETICS LA English DT Article DE juvenile polyps; familial cancer; gastrointestinal neoplasms; juvenile polyposis; chromosome abnormality; del(10)(q22.3q24.1) ID INTERSTITIAL DELETION; LONG ARM; CHROMOSOME-10; PATHOGENESIS; CARCINOMA; CANCER; COLI AB Juvenile polyps are the most frequent gastrointestinal polyps with a malignant potential for which the genetic basis is unknown, Juvenile polyps, with a normal epithelium but hypertrophic lamina propria, are histologically quite distinct from adenomatous polyps which have dysplastic changes in epithelial nuclei. Furthermore, the adenomatous polyposis coli (APC) gene on Chr 5, mutated somatically in adenomatous polyps and mutated in the germline of patients with familial adenomatous polyposis, is not linked to hereditary juvenile polyposis, We provide the first report indicating that a tumor suppressor gene associated with juvenile polyposis may be located at 10q22.3q24.1, Cytogenetic studies of a patient with juvenile polyposis and multiple congenital abnormalities of the head, extremities, and abdomen revealed a de novo interstitial deletion of Chr 10 as the only defect, del(10)(10q22.3q24.1). Am. J. Med. Genet. 70:361-364, 1997. (C) 1997 Wiley-Liss, Inc. C1 UNIV WISCONSIN,DEPT MED,GASTROENTEROL SECT,MADISON,WI. UNIV WISCONSIN,DEPT GENET,MADISON,WI 53706. UNIV WISCONSIN,DEPT PEDIAT,MADISON,WI 53706. UNIV WISCONSIN,CTR COMPREHENS CANC,MADISON,WI. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. FU NCI NIH HHS [U01 CA59352, P30 CA14520] NR 29 TC 40 Z9 40 U1 1 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0148-7299 J9 AM J MED GENET JI Am. J. Med. Genet. PD JUN 27 PY 1997 VL 70 IS 4 BP 361 EP 364 DI 10.1002/(SICI)1096-8628(19970627)70:4<361::AID-AJMG6>3.0.CO;2-W PG 4 WC Genetics & Heredity SC Genetics & Heredity GA XC369 UT WOS:A1997XC36900006 PM 9182775 ER PT J AU Salvatore, M Morzunov, S Schwemmle, M Lipkin, WI Bunney, WE Cotman, CW Even, C Hatalski, CG Potkin, SG Portlance, ML Nichol, ST Tourtelloutte, WW Riederer, P terMeulen, V AF Salvatore, M Morzunov, S Schwemmle, M Lipkin, WI Bunney, WE Cotman, CW Even, C Hatalski, CG Potkin, SG Portlance, ML Nichol, ST Tourtelloutte, WW Riederer, P terMeulen, V TI Borna disease virus in brains of North American and European people with schizophrenia and bipolar disorder SO LANCET LA English DT Article C1 UNIV CALIF IRVINE,DEPT ANAT & NEUROBIOL,IRVINE,CA 92697. UNIV CALIF IRVINE,DEPT MICROBIOL & MOL GENET,IRVINE,CA 92697. UNIV CALIF IRVINE,DEPT PSYCHIAT & HUMAN BEHAV,IRVINE,CA 92697. CTR DIS CONTROL & PREVENT,SPECIAL PATHOGENS BRANCH,DIV VIRAL & RICKETTSIAL DIS,ATLANTA,GA. W LOS ANGELES VET AFFAIRS MED CTR,NATL NEUROL RES SPECIMEN BANK,LOS ANGELES,CA 90073. UNIV NEVADA,RENO,NV 89557. UNIV WURZBURG,DEPT PSYCHIAT,D-8700 WURZBURG,GERMANY. UNIV WURZBURG,INST VIROL & IMMUNBIOL,D-8700 WURZBURG,GERMANY. RP Salvatore, M (reprint author), UNIV CALIF IRVINE,DEPT NEUROL,IRVINE,CA 92697, USA. RI Potkin, Steven/A-2021-2013; salvatore, mirella/K-6691-2016 OI salvatore, mirella/0000-0002-8296-0376 NR 5 TC 78 Z9 86 U1 1 U2 3 PU LANCET LTD PI LONDON PA 42 BEDFORD SQUARE, LONDON, ENGLAND WC1B 3SL SN 0140-6736 J9 LANCET JI Lancet PD JUN 21 PY 1997 VL 349 IS 9068 BP 1813 EP 1814 DI 10.1016/S0140-6736(05)61693-5 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA XF735 UT WOS:A1997XF73500017 PM 9269221 ER PT J AU Rettig, MB Ma, HJ Vescio, RA Pold, M Schiller, G Belson, D Savage, A Nishikubo, C Wu, C Fraser, J Said, JW Berenson, JR AF Rettig, MB Ma, HJ Vescio, RA Pold, M Schiller, G Belson, D Savage, A Nishikubo, C Wu, C Fraser, J Said, JW Berenson, JR TI Kaposi's sarcoma-associated herpesvirus infection of bone marrow dendritic cells from multiple myeloma patients SO SCIENCE LA English DT Article ID MONOCLONAL GAMMOPATHY; DNA-SEQUENCES; UNDETERMINED SIGNIFICANCE; INTERLEUKIN-6; CYTOKINES; RECEPTOR AB Kaposi's sarcoma-associated herpesvirus (KSHV) was found in the bone marrow dendritic cells of multiple myeloma patients but not in malignant plasma cells or bone marrow dendritic cells from normal individuals or patients with other malignancies. In addition the virus was detected in the bone marrow dendritic cells from two out of eight patients with monoclonal gammopathy of undetermined significance (MGUS), a precursor to myeloma. Viral interleukin-6, the human homolog of which is a growth factor for myeloma, was found to be transcribed in the myeloma bone marrow dendritic cells. KSHV may be required for transformation from MGUS to myeloma and perpetuate the growth of malignant plasma cells. C1 UNIV CALIF LOS ANGELES,SCH MED,DIV HEMATOL & ONCOL,LOS ANGELES,CA 90095. UNIV CALIF LOS ANGELES,SCH MED,JONSSON COMPREHENS CANC CTR,LOS ANGELES,CA 90095. UNIV CALIF LOS ANGELES,SCH MED,DEPT PATHOL,LOS ANGELES,CA 90095. RP Rettig, MB (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,DIV HEMATOL ONCOL,11301 WILSHIRE BLVD,111-H,LOS ANGELES,CA 90073, USA. RI Fraser, John/I-7309-2013 NR 40 TC 396 Z9 409 U1 0 U2 0 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD JUN 20 PY 1997 VL 276 IS 5320 BP 1851 EP 1854 DI 10.1126/science.276.5320.1851 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA XF103 UT WOS:A1997XF10300046 PM 9188529 ER PT J AU Schwartz, MW Seeley, RJ AF Schwartz, MW Seeley, RJ TI Neuroendocrine responses to starvation and weight loss SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Review ID CORTICOTROPIN-RELEASING FACTOR; LUTEINIZING-HORMONE SECRETION; NEUROPEPTIDE GENE-EXPRESSION; CENTRAL-NERVOUS-SYSTEM; BODY-WEIGHT; PARAVENTRICULAR NUCLEUS; OBESE GENE; FOOD-DEPRIVATION; ENERGY-BALANCE; MESSENGER-RNA C1 UNIV WASHINGTON, HARBORVIEW MED CTR, DEPT MED, SEATTLE, WA 98104 USA. UNIV WASHINGTON, HARBORVIEW MED CTR, DEPT PSYCHOL, SEATTLE, WA 98104 USA. RP Schwartz, MW (reprint author), VET AFFAIRS PUGET SOUND HLTH CARE SYST, 1660 S COLUMBIAN WAY, SEATTLE, WA 98108 USA. RI Schwartz, Michael/H-9950-2012 FU NIDDK NIH HHS [DK-12829]; NINDS NIH HHS [NS32273] NR 88 TC 207 Z9 211 U1 1 U2 6 PU MASSACHUSETTS MEDICAL SOC PI WALTHAM PA WALTHAM WOODS CENTER, 860 WINTER ST,, WALTHAM, MA 02451-1413 USA SN 0028-4793 EI 1533-4406 J9 NEW ENGL J MED JI N. Engl. J. Med. PD JUN 19 PY 1997 VL 336 IS 25 BP 1802 EP 1811 PG 10 WC Medicine, General & Internal SC General & Internal Medicine GA XE485 UT WOS:A1997XE48500007 PM 9187072 ER PT J AU Goldstein, B Hammond, M AF Goldstein, B Hammond, M TI Physical medicine and rehabilitation SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Article C1 UNIV WASHINGTON,SEATTLE,WA 98195. RP Goldstein, B (reprint author), VA PUGET SOUND HLTH CARE SYST,SEATTLE,WA 98195, USA. NR 15 TC 4 Z9 5 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD JUN 18 PY 1997 VL 277 IS 23 BP 1891 EP 1892 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA XD544 UT WOS:A1997XD54400039 PM 9185820 ER PT J AU OBrien, WA Hartigan, PM Daar, ES Simberkoff, MS Hamilton, JD AF OBrien, WA Hartigan, PM Daar, ES Simberkoff, MS Hamilton, JD TI Changes in plasma HIV RNA levels and CD4(+) lymphocyte counts predict both response to antiretroviral therapy and therapeutic failure SO ANNALS OF INTERNAL MEDICINE LA English DT Article ID IMMUNODEFICIENCY-VIRUS INFECTION; ZIDOVUDINE; QUANTITATION; TUBERCULOSIS; REPLICATION; VACCINATION AB Background: Markers are needed for assessing response to antiretroviral therapy over time. The CD4(+) lymphocyte count is one such surrogate, but it is relatively weak. Objective: To assess the association of changes in plasma human immunodeficiency virus (HIV) RNA level and CD4(+) lymphocyte count with progression to the acquired immunodeficiency syndrome (AIDS). Design: Analysis of data from a subset of patients in a multicenter, randomized, clinical trial. Setting: Six Veterans Affairs medical centers and one U.S. Army medical center. Patients: 270 symptomatic HIV-infected patients from the Veterans Affairs Cooperative Study on AIDS. Intervention: Patients were randomly assigned to receive zidovudine or placebo initially; a cross-over protocol was established for patients receiving placebo who had disease progression. Measurements: Reverse transcriptase polymerase chain reaction on cryopreserved plasma samples, previously obtained CD4(+) lymphocyte counts, and clinical events. Results: For each decrease of 0.5 log(10) copies/mL in plasma HIV RNA level, averaged over the 6 months after randomization, the relative risk (RR) for progression to AIDS was 0.67 (P < 0.001). In a subset of 70 treated patients with long-term follow-up, a return to baseline plasma HIV RNA levels within 6 months of randomization was associated with progression to AIDS (RR, 4.28; P = 0.004). Plasma HIV RNA levels or CD4(+) lymphocyte counts over time were more strongly associated with progression to AIDS than were baseline levels or counts. Conclusions: An adequate virologic response after initiation of antiretroviral therapy seems to require a decrease in plasma HIV RNA level of at least 0.5 log(10) copies/mL that is sustained for at least 6 months. The independent relation between plasma HIV RNA level and CD4(+) lymphocyte count over time and clinical outcome suggests that the measurement of plasma HIV RNA level, in addition to the CD4(+) lymphocyte count, has a role in guiding the management of antiretroviral therapy. C1 W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, SCH MED, LOS ANGELES, CA USA. W HAVEN VET AFFAIRS MED CTR, W HAVEN, CT USA. CEDARS SINAI MED CTR, NEW YORK, NY USA. VET AFFAIRS MED CTR, NEW YORK, NY USA. NYU, SCH MED, NEW YORK, NY USA. DUKE UNIV, MED CTR, DIV INFECT DIS & INT HLTH, DURHAM, NC 27710 USA. VET AFFAIRS MED CTR, DURHAM, NC 27705 USA. RES CTR AIDS & HIV INFECT, DURHAM, NC USA. NR 30 TC 227 Z9 229 U1 0 U2 6 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD JUN 15 PY 1997 VL 126 IS 12 BP 939 EP 945 PG 7 WC Medicine, General & Internal SC General & Internal Medicine GA XE549 UT WOS:A1997XE54900005 PM 9182470 ER PT J AU Meredith, M Li, GD Metz, SA AF Meredith, M Li, GD Metz, SA TI Inhibition of calcium-induced insulin secretion from intact HIT-T15 or INS-1 beta cells by GTP depletion SO BIOCHEMICAL PHARMACOLOGY LA English DT Article DE GTP; insulin secretion; Ca2+; exocytosis; guanine; adenine; mycophenolate ID RAT ISLETS; MYCOPHENOLATE MOFETIL; GUANINE-NUCLEOTIDES; PANCREATIC-ISLETS; B-CELLS; RELEASE; GLUCOSE; ESTABLISHMENT; ACTIVATION; METABOLISM AB Using intact rat islets, we previously observed that GTP depletion (achieved through the use of mycophenolic acid or other synthesis inhibitors) impedes nutrient- but not K+-induced insulin secretion. It was concluded that a proximal nutrient-dependent step in stimulus-secretion coupling (but not the process of Ca2+-induced exocytosis itself) is modulated by ambient GTP levels. To examine Ca2+-dependent steps further in intact beta cells, INS-1 cells (which synthesize GTP and ATP similarly to rat islets) and HIT-T15 cells (whose synthesis of purine nucleotides is different) were studied following cell culture for 1-18 hr in various concentrations of mycophenolic acid (MPA) or mizoribine (MZ). Both agents profoundly reduced GTP content (mean: -78%) and lowered the GTP/GDP ratio by an average of -73%; concomitantly, MPA or MZ reduced insulin secretion induced by 10 mM glucose, 30 or 40 mM KCl, or 100 mu M tolbutamide, independent of any changes in cell viability, insulin content, ATP content, the ATP/ADP ratio, or cytosolic free Ca2+ concentrations. In INS-1 cells (which appear to have normal nucleobase transport and ''salvage'' pathway activities), guanine (but not adenine) restored GTP content, the GTP/GDP ratio, and Ca2+-induced secretion. In HIT cells, the phosphoribosylation of exogenous guanine or hypoxanthine is defective; however, provision of 500 mu M guanosine (but not adenosine) reversed the effects of MPA. We conclude that, at least in certain situations, a requisite role for GTP in the distal step(s) of exocytosis can be demonstrated. (C) 1997 Elsevier Science Inc. C1 UNIV WISCONSIN,ENDOCRINOL SECT,MADISON,WI. UNIV WISCONSIN,DEPT MED,MADISON,WI. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. FU NIDDK NIH HHS [DK 37312] NR 32 TC 18 Z9 23 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0006-2952 J9 BIOCHEM PHARMACOL JI Biochem. Pharmacol. PD JUN 15 PY 1997 VL 53 IS 12 BP 1873 EP 1882 DI 10.1016/S0006-2952(97)00057-9 PG 10 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA XN780 UT WOS:A1997XN78000012 PM 9256162 ER PT J AU Ripple, MO Pickhardt, PA Wilding, G AF Ripple, MO Pickhardt, PA Wilding, G TI Alteration in gamma-glutamyl transpeptidase activity and messenger RNA of human prostate carcinoma cells by androgen SO CANCER RESEARCH LA English DT Article ID TRANSFERASE TRANSPEPTIDASE; EPITHELIAL-CELLS; RAT EPIDIDYMIS; EXPRESSION; GLUTATHIONE; HEPATOCARCINOGENESIS; DIFFERENTIATION; LOCALIZATION; HEPATOMA; KIDNEY AB Concentrations of the synthetic androgen R1881 that correspond to physiologically relevant concentrations of 5 alpha-dihydrotestosterone are capable of altering the activity of gamma-glutamyl transpeptidase (GGT) in human prostate carcinoma cells. GGT activity of the androgen-responsive prostate dancer cell line LNCaP increases >50% above that of the control after a 72-h exposure to 1 nM R1881. This elevation in GGT activity occurs as early as 48 h after treatment and is maintained for at least 96 h. Loss of glutathione (GSH) from media and accumulation of intracellular GSH of cells pretreated with 1 nM R1881 occur at a higher rate than in control cells, suggesting that a greater rate of GSH salvage is associated with the increased GGT activity. Immunohistochemical staining detects an increase in GGT-positive staining in cells treated with 1 nM R1881 for 72 h. Steady-state mRNA levels for GGT are elevated above those of the control 24-72 h after treatment. R1881 has no effect on the GGT activity of the androgen-independent prostate cell line DU145. Growth of LNCaP but not DU145 cells is inhibited by 1 nM R1881 compared to that of the control. Inhibitors of GGT activity, acivicin and serine-borate, are capable of dampening or blocking the effect of R1881 on growth. Growth of LNCaP cells treated with 1 nM R1881 plus 100 mM glycylglycine, a stimulator of GGT activity, is inhibited to a greater extent than the growth of LNCaP cells treated with R1881 alone. These data demonstrate that androgens can elevate GGT activity and increase GGT mRNA and protein levels in human prostate carcinoma cells. In addition, compounds able to alter GGT activity are capable of altering androgen-related growth effects. C1 UNIV WISCONSIN,CTR COMPREHENS CANC,DEPT MED,ENVIRONM TOXICOL CTR,MED ONCOL SECT,MADISON,WI 53792. UNIV WISCONSIN,WILLIAM S MIDDLETON MEM VET ADM HOSP,MADISON,WI 53792. FU NIEHS NIH HHS [5-T32-ES07015-18] NR 41 TC 13 Z9 13 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD JUN 15 PY 1997 VL 57 IS 12 BP 2428 EP 2433 PG 6 WC Oncology SC Oncology GA XD800 UT WOS:A1997XD80000023 PM 9192821 ER PT J AU Sawicki, M Arnold, E Ebrahimi, S Duell, T Jin, S Wood, T Chakrabarti, R Peters, J Wan, Y Samara, G Weier, HUG Udar, N Passaro, E Srivatsan, ES AF Sawicki, M Arnold, E Ebrahimi, S Duell, T Jin, S Wood, T Chakrabarti, R Peters, J Wan, Y Samara, G Weier, HUG Udar, N Passaro, E Srivatsan, ES TI A transcript map encompassing the multiple endocrine neoplasia type-1 (MEN1) locus on chromosome 11q13 SO GENOMICS LA English DT Article ID HUMAN Y-CHROMOSOME; CDNA SELECTION; PARATHYROID TUMORS; LINKAGE ANALYSIS; SMALL REGION; GENE; MARKERS; PROTEIN; DNA; IDENTIFICATION AB A transcription map of a 1200-kb region encompassing the MEN1 locus was constructed by direct cDNA selection and mapping ESTs. A total of 29 genes were mapped. Ten transcripts were identified by cDNA selection of a focused 300-kb genomic region telomeric to the MEN1 consensus region. Since many of the sequences cloned by cDNA selection also identified ESTs from the region, 19 additional RH-mapped ESTs were mapped to the entire contig region by CR amplification of genomic clones. Nine known genes, 2 putative human homologues to mouse genes, and is novel transcripts map to the region. Transcripts that map to the MEN1 interval PYGM-D11S449 include SGC35223, IB1256, AA147620, ZFM1, FAU, and CAPN1. The latter 3 known genes have already been excluded as candidate MEN1 genes. The 2 putative human homologues of mouse genes Ltbp2 and Spa-1 may be candidate tumor suppressor genes, but they map telomeric to D11S449. Although both of these genes map outside the MEN1 consensus region they may play a role in sporadic endocrine tumors independent of the MEN1 gene or in other tumors, such as breast cancer, that have loss of heterozygosity within this region. (C) 1997 Academic Press. C1 UNIV CALIF LOS ANGELES,DEPT PATHOL,LOS ANGELES,CA 90073. UNIV CALIF BERKELEY,LAWRENCE BERKELEY LAB,DIV LIFE SCI,BERKELEY,CA 94720. UNIV MUNICH,MED KLIN 3,D-80539 MUNICH,GERMANY. CHILDRENS HOSP LOS ANGELES,DEPT PATHOL,LOS ANGELES,CA 90027. HARBOR UCLA MED CTR,DEPT PATHOL,TORRANCE,CA 90509. RP Sawicki, M (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,DEPT SURG W112,MOL BIOL CORE UNIT,LOS ANGELES,CA 90073, USA. NR 46 TC 11 Z9 11 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0888-7543 J9 GENOMICS JI Genomics PD JUN 15 PY 1997 VL 42 IS 3 BP 405 EP 412 DI 10.1006/geno.1997.4773 PG 8 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA XG519 UT WOS:A1997XG51900006 PM 9205112 ER PT J AU Li, XL Sandoval, D Freeberg, L Carter, RH AF Li, XL Sandoval, D Freeberg, L Carter, RH TI Role of CD19 tyrosine 391 in synergistic activation of B lymphocytes by coligation of CD19 and membrane Ig SO JOURNAL OF IMMUNOLOGY LA English DT Article ID PROTEIN-KINASE; T-CELL; MAP KINASE; COMPLEMENT RECEPTOR; PHOSPHATIDYLINOSITOL 3-KINASE; SELECTIVE ACTIVATION; SIGNALING PATHWAY; EXCHANGE ACTIVITY; CROSS-LINKING; MEK KINASE AB CD19 and CD21,which form a complex on B lymphocytes, are required for normal Ab responses to T-dependent Ags, Coligation of the CD21/CD19 complex with membrane IgM (mIgM) powerfully enhances B cell activation in vitro and in vivo, To determine how CD19-mIgM synergy is produced, we examined immediate and downstream signaling events after ligation of either complex alone or after CD19-mIgM coligation in normal and lymphoblastoid B cells, Ligation of mIgM alone is known to result in tyrosine phosphorylation of CD19 and association of CD19 with phosphatidylinositol 3-kinase and Vav, and these events are not enhanced by coligation, In contrast, tyrosine phosphorylation of phosphatidylinositol 3-kinase and Vav is markedly enhanced after CD19-mIgM coligation. Coligation also results in synergistic, prolonged enhancement of mitogen-activated protein kinase activity, relative to ligation of either receptor complex alone, Mutation of CD19 Y391, the site at which Vav binds, but not mutation of CD19 Y482 and Y513, the sites of association with phosphatidylinositol 3-kinase, blocks the enhancement of mitogen-activated protein kinase activation, These findings suggest that the synergistic enhancement of B cell activation following CD19-mIgM coligation results from greater tyrosine phosphorylation of Vav and Vav-dependent enhanced activation of mitogen-activated protein kinases. C1 UNIV ALABAMA,LHRB 409,DEPT MED,BIRMINGHAM,AL 35294. UNIV ALABAMA,DEPT MICROBIOL,BIRMINGHAM,AL 35294. BIRMINGHAM VET AFFAIRS MED CTR,BIRMINGHAM,AL 35294. FU NIAMS NIH HHS [T32 AR07450] NR 50 TC 50 Z9 50 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD JUN 15 PY 1997 VL 158 IS 12 BP 5649 EP 5657 PG 9 WC Immunology SC Immunology GA XE749 UT WOS:A1997XE74900011 PM 9190913 ER PT J AU Goldzweig, CL Mittman, BS Carter, GM Donyo, T Brook, RH Lee, P Mangione, CM AF Goldzweig, CL Mittman, BS Carter, GM Donyo, T Brook, RH Lee, P Mangione, CM TI Variations in cataract extraction rates in Medicare prepaid and fee-for-service settings SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Article ID HEALTH-MAINTENANCE ORGANIZATIONS; QUALITY-OF-LIFE; ELDERLY PATIENTS; VISUAL FUNCTION; RISK-FACTORS; SURGERY; VISION; CARE; PHYSICIANS; OUTCOMES AB Objective.-To compare rates of cataract extraction in 2 prepaid health settings and in traditional fee-for-service (FFS) settings. Design.-A cross-sectional analysis using 1993 health maintenance organization (HMO) Medicare claims and encounter files, the Health Care Financing Administration (HCFA) 5% Medicare Part B provider/supplier file, and the HCFA October 1992 100% Medicare population file. Setting.-Southern California Medicare FFS settings and the staff-model and independent practice association (IPA) plans of a large California HMO. Patients.-1993 Medicare beneficiaries aged 65 years and older, The study included 43 387 staff-model HMO enrollees, 19 050 IPA enrollees, and 47 150 FFS beneficiaries (a 5% sample of all Southern California FFS beneficiaries). Main Outcome Measure.-Age and risk-factor adjusted rates of cataract extraction per 1000 beneficiary-years. Results.-After controlling for age, sex, and diabetes mellitus status, FFS beneficiaries were twice as likely to undergo cataract extraction as were prepaid beneficiaries (P<.01). Female FFS beneficiaries were nearly twice as likely to undergo the procedure as were male FFS beneficiaries (P<.001); there were no extraction rate differences by sex in the prepaid settings. Conclusion.-Because of the potential implications for vision care in the elderly, the significantly different rates of cataract extraction in FFS and prepaid settings warrant further clinical investigation to determine whether there is overuse in FFS vs underuse in prepaid settings. Such investigations must assess the appropriateness of cataract surgery by evaluating its use relative to clinical need. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90024. RAND CORP,SANTA MONICA,CA. UNIV SO CALIF,SCH MED,DEPT OPHTHALMOL,LOS ANGELES,CA 90033. RP Goldzweig, CL (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,DIV GEN INTERNAL MED,11301 WILSHIRE BLVD,111G,LOS ANGELES,CA 90073, USA. OI Lee, Paul/0000-0002-3338-136X FU NIA NIH HHS [K08 AG000605]; OHS HRSA HHS [ST32PE19001] NR 28 TC 60 Z9 60 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD JUN 11 PY 1997 VL 277 IS 22 BP 1765 EP 1768 DI 10.1001/jama.277.22.1765 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA XC499 UT WOS:A1997XC49900028 PM 9178788 ER PT J AU Badgett, RG Lucey, CR Mulrow, CD AF Badgett, RG Lucey, CR Mulrow, CD TI Can the clinical examination diagnose left-sided heart failure in adults? SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Review ID VENTRICULAR EJECTION FRACTION; ACUTE MYOCARDIAL-INFARCTION; PULMONARY-ARTERY CATHETERIZATION; CRITICALLY ILL PATIENTS; CHEST-X-RAY; RADIONUCLIDE VENTRICULOGRAPHY; DIASTOLIC DYSFUNCTION; SYSTOLIC FUNCTION; ECHOCARDIOGRAPHIC INSIGHTS; VALSALVA MANEUVER AB We systematically reviewed the literature to ascertain how well clinicians determine the probability and type of left-sided heart failure in their patients, Left-sided heart failure is characterized by decreased left ventricular ejection fraction or increased filling pressure. The type of heart failure determines optimal treatment, Systolic dysfunction exists when ejection fraction is reduced, Diastolic dysfunction is presumed to be present when filling pressure is increased with a normal ejection fraction and without another explanatory diagnosis, Many findings are associated with heart failure, and wide variation exists in clinicians' ability to detect these findings. The best findings for detecting increased filling pressure are jugular venous distention and radiographic redistribution, The best findings for detecting systolic dysfunction are abnormal apical impulse, radiographic cardiomegaly, and q waves or left bundle branch block on an electrocardiogram. Diastolic dysfunction is especially difficult to diagnose, but is associated with an elevated blood pressure during heart failure. C1 AUDIE L MURPHY MEM VET ADM MED CTR, DEPT MED, SAN ANTONIO, TX 78284 USA. WASHINGTON HOSP CTR, WASHINGTON, DC 20010 USA. RP Badgett, RG (reprint author), UNIV TEXAS, HLTH SCI CTR, DEPT MED, DIV GEN MED, 4200 FLOYD CURL DR, SAN ANTONIO, TX 78284 USA. OI Badgett, Robert/0000-0003-2888-3303 NR 89 TC 136 Z9 139 U1 0 U2 4 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60654-0946 USA SN 0098-7484 EI 1538-3598 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD JUN 4 PY 1997 VL 277 IS 21 BP 1712 EP 1719 DI 10.1001/jama.277.21.1712 PG 8 WC Medicine, General & Internal SC General & Internal Medicine GA XB088 UT WOS:A1997XB08800034 PM 9169900 ER PT J AU Tanaka, S Guth, PH Carryl, OR Kaunitz, JD AF Tanaka, S Guth, PH Carryl, OR Kaunitz, JD TI Cytoprotective effect of bismuth subsalicylate in indomethacin-treated rats is associated with enhanced mucus bismuth concentration SO ALIMENTARY PHARMACOLOGY & THERAPEUTICS LA English DT Article ID GASTRIC-MUCOSAL LESIONS; INTRACELLULAR PH; ALKALINE SECRETION; GEL THICKNESS; IN-VIVO; SUBCITRATE; PATHOGENESIS; PROSTAGLANDIN-E2; STIMULATION; MECHANISM AB Background: Bismuth compounds prevent gastric injury from the short-term administration of nonsteroidal antiinflammatory drugs. We studied the mechanisms underlying the gastroprotective actions of bismuth subsalicylate against indomethacin-induced injury in rats. Methods: An in vivo microscopic technique was used in which acid output, surface cell intracellular pH (pH(i)), gastric mucus gel thickness and mucosaol blood flow were measured simultaneously. Concentrations of bismuth in mucus were measured by atomic absorption. Results: Indomethacin (60 mg/kg) significantly thinned the mucus gel layer and augmented the decrease of pH(i) during luminal acid superfusion, consistent with a weakened gastric mucosal barrier to acid. Bismuth subsalicylate partially reversed this effect of indomethacin on pH(i), consistent with gastroprotection. Neither a prostaglandin-inhibiting but non-injurious dose of indomethacin (5 mg/kg), bismuth subsalicylate, or their combination affected mucus gel thickness or pH(i) homeostasis. In separate experiments, indomethacin (60 mg/kg) significantly increased gastric mucus bismuth concentration in rats given bismuth subsalicylate. Conclusion: Bismuth accumulation in the gastric mucus during the evolution of mucosal injury may play an important role in the gastroprotective effect of bismuth subsalicylate against indomethacin injury. C1 W LOS ANGELES VET AFFAIRS MED CTR,MED SERV,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90024. PROCTER & GAMBLE CO,HLTH CARE PROD,CINCINNATI,OH. NR 33 TC 7 Z9 8 U1 0 U2 1 PU BLACKWELL SCIENCE LTD PI OXFORD PA P O BOX 88, OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0NE SN 0269-2813 J9 ALIMENT PHARM THERAP JI Aliment. Pharmacol. Ther. PD JUN PY 1997 VL 11 IS 3 BP 605 EP 612 DI 10.1046/j.1365-2036.1997.00170.x PG 8 WC Gastroenterology & Hepatology; Pharmacology & Pharmacy SC Gastroenterology & Hepatology; Pharmacology & Pharmacy GA XF543 UT WOS:A1997XF54300026 PM 9218090 ER PT J AU Herbert, V AF Herbert, V TI Destroying immune homeostasis in normal adults with antioxidant supplements SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Letter C1 BRONX VET ADM MED CTR,NEW YORK,NY 10468. RP Herbert, V (reprint author), MT SINAI MED CTR,NEW YORK,NY 10468, USA. NR 9 TC 3 Z9 3 U1 0 U2 0 PU AMER SOC CLINICAL NUTRITION PI BETHESDA PA 9650 ROCKVILLE PIKE, SUBSCRIPTIONS, RM L-2310, BETHESDA, MD 20814-3998 SN 0002-9165 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JUN PY 1997 VL 65 IS 6 BP 1901 EP 1901 PG 1 WC Nutrition & Dietetics SC Nutrition & Dietetics GA XB663 UT WOS:A1997XB66300035 PM 9174495 ER PT J AU Writer, JV Kang, H Lee, KL Kelley, PW AF Writer, JV Kang, H Lee, KL Kelley, PW TI Pre-war hospitalization as a risk factor for enrollment on a Persian Gulf Registry. SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Meeting Abstract C1 WALTER REED ARMY MED CTR,WALTER REED ARMY INST RES,WASHINGTON,DC 20307. US DEPT VET AFFAIRS,WASHINGTON,DC 20036. NR 0 TC 1 Z9 1 U1 0 U2 0 PU JOHNS HOPKINS UNIV SCHOOL HYGIENE PUB HEALTH PI BALTIMORE PA 111 MARKET PLACE, STE 840, BALTIMORE, MD 21202-6709 SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD JUN 1 PY 1997 VL 145 IS 11 SU S BP 340 EP 340 PG 1 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA XA896 UT WOS:A1997XA89600338 ER PT J AU Yousfi, MM ElZimaity, HMT Cole, RA Genta, RM Graham, DY AF Yousfi, MM ElZimaity, HMT Cole, RA Genta, RM Graham, DY TI Comparison of agar gel (CLOtest) or reagent strip (PyloriTek) rapid urease tests for detection of Helicobacter pylori infection SO AMERICAN JOURNAL OF GASTROENTEROLOGY LA English DT Article ID CAMPYLOBACTER-PYLORI; DIAGNOSTIC-TESTS; COLONIZATION; GASTRITIS; HISTOLOGY; DISEASE; CULTURE AB Objective: Rapid urease tests (RUTs) are used commonly as a convenient method to detect Helicobacter pylori infection, New rapid tests have been commercially available with promotional literature suggesting enhanced utility, We compared CLOtest to a new reagent strip RUT, PyloriTek, Methods: Gastric antral mucosal biopsy specimens were obtained from 102 patients for comparison between CLOtest and PyloriTek (204 specimens), Biopsy specimens obtained from a nearby area were stained using the Genta stain for determination of H. pylori status, The RUT to be used first was selected randomly, Results: Sixty-five of the 102 patients had peptic ulcer disease, two had gastric cancer, and 35 had dyspepsia; 61 patients had active H. pylori infection, There were one false-negative and three false-positive CLOtest results, compared with one false-negative and 13 false-positive PyloriTek results (p < 0.02 for incorrect categorization with PyloriTek), Sensitivity and specificity were 98 and 92% compared with 98 and 68% for CLOtest and PyloriTek, respectively, An erroneous categorization of H. pylori status occurred in 3.9% (95% confidence interval [CI]: 1-9.7%) with CLOtest compared with 13.7% (95% CI: 7.7-22%) with PyloriTek, When the PyloriTek was scored at 1 h (0-1 h) after obtaining the specimen, the accuracy improved; erroneous categorization of H. pylori status occurred in only 2.9% (95% CI: 0.6-8.3%), Conclusion: Used according to manufacturer instructions, the new reagent strip RUT PyloriTek has too many false-positive results for use in a clinical situation, In contrast, when the test was interpreted within 1 h, accuracy was comparable to that of CLOtest. C1 VET AFFAIRS MED CTR 111D,DEPT MED,HOUSTON,TX 77030. VET AFFAIRS MED CTR,DEPT PATHOL,HOUSTON,TX 77030. VET AFFAIRS MED CTR,DIV MOL VIROL,HOUSTON,TX 77030. BAYLOR COLL MED,HOUSTON,TX 77030. NR 26 TC 19 Z9 20 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-9270 J9 AM J GASTROENTEROL JI Am. J. Gastroenterol. PD JUN PY 1997 VL 92 IS 6 BP 997 EP 999 PG 3 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA XC373 UT WOS:A1997XC37300018 PM 9177518 ER PT J AU Small, GW AF Small, GW TI Geriatric psychiatry: Key research topics for clinicians - Murphy,E, Alexopoulos,G SO AMERICAN JOURNAL OF GERIATRIC PSYCHIATRY LA English DT Book Review C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,GERIATR PSYCHIAT FELLOWSHIP TRAINING PROGRAM,LOS ANGELES,CA 90024. RP Small, GW (reprint author), UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA 90024, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 1064-7481 J9 AM J GERIAT PSYCHIAT JI Am. J. Geriatr. Psychiatr. PD SUM PY 1997 VL 5 IS 3 BP 271 EP 272 PG 2 WC Geriatrics & Gerontology; Gerontology; Psychiatry SC Geriatrics & Gerontology; Psychiatry GA XJ829 UT WOS:A1997XJ82900014 ER PT J AU Macones, GA Asch, DA AF Macones, GA Asch, DA TI Costs, true costs, and whose costs in economic analyses in medicine? SO AMERICAN JOURNAL OF MANAGED CARE LA English DT Article ID BLOOD ACID-BASE; GAS AB Cost-effectiveness analyses of clinical practices are becoming more common in the development of health policy. However such analyses can be based on misconceptions and flawed assumptions, leading to flawed policies, We argue that such is the case with the recent recommendations for routine measurement of umbilical cord gases at delivery, a policy based on the assumption that this clinical strategy will pay for itself by reduced malpractice awards, As we demonstrate, this argument reflects the physicians' perspective, not that of society or of patients. It also ignores the fact that malpractice awards are largely transfer payments, not costs of healthcare. C1 UNIV PENN,LEONARD DAVIS INST HLTH ECON,PHILADELPHIA,PA 19104. UNIV PENN,SCH MED,DEPT OBSTET & GYNECOL,PHILADELPHIA,PA 19104. VET AFFAIRS MED CTR,PHILADELPHIA,PA. UNIV PENN,SCH MED,DIV GEN INTERNAL MED,PHILADELPHIA,PA 19104. RP Macones, GA (reprint author), UNIV PENN,CTR CLIN EPIDEMIOL & BIOSTAT,ROOM 901,BLOCKLEY HALL,423 GUARDIAN DR,PHILADELPHIA,PA 19104, USA. NR 11 TC 2 Z9 2 U1 0 U2 1 PU AMER MED PUBLISHING, M W C COMPANY PI OLD BRIDGE PA 1816 ENGLISHTOWN RD, STE 101, OLD BRIDGE, NJ 08857 SN 1088-0224 J9 AM J MANAG C JI Am. J. Manag. Care PD JUN PY 1997 VL 3 IS 6 BP 915 EP 917 PG 3 WC Health Care Sciences & Services; Health Policy & Services; Medicine, General & Internal SC Health Care Sciences & Services; General & Internal Medicine GA YB220 UT WOS:A1997YB22000006 PM 10170295 ER PT J AU CoupayeGerard, B Zuckerman, JB Duncan, P Bortnik, A Avery, DI Ernst, SA Kleyman, TR AF CoupayeGerard, B Zuckerman, JB Duncan, P Bortnik, A Avery, DI Ernst, SA Kleyman, TR TI Delivery of newly synthesized Na+-K+-ATPase to the plasma membrane of A6 epithelia SO AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY LA English DT Article DE sodium pump; sorting; intracellular trafficking; biotin; sodium-potassium adenosinetriphosphatase ID CELL-SURFACE DELIVERY; INTRACELLULAR-TRANSPORT; BETA-SUBUNITS; ALPHA-SUBUNIT; IMMUNOCYTOCHEMICAL LOCALIZATION; CYTOPLASMIC DOMAIN; KIDNEY-CELLS; MDCK CELLS; NA,K-ATPASE; EXPRESSION AB Na+-K+-ATPase is localized to the basolateral cell surface of most epithelial cells. Conflicting results regarding the intracellular trafficking of Na+-K+- ATPase in Madin-Darby canine kidney cells have been reported, with delivery to both apical and basolateral membranes or exclusively to the basolateral cell surface. We examined the delivery and steady-state distribution of Na+-K+-ATPase in the amphibian epithelial cell line A6 using an antibody raised against Na+-K+-ATPase alpha-subunit and sulfo-N-hydroxysuccinimidobiotin to tag cell surface proteins. The steady-state distribution of the Na+-K+-ATPase was basolateral, as confirmed by immunocytochemistry. Delivery of newly synthesized Na+-K+-ATPase to the cell surface was examined using [S-35]methionine and [S-35]cysteine in a pulse-chase protocol. After a 20-min pulse, the alpha-subunit and core glycosylated beta-subunit were present at both apical and basolateral cell surfaces. The alpha-subunit and core glycosylated beta-subunit delivered to the apical cell surface were degraded within 2 h. Mature alpha/beta-heterodimer was found almost exclusively at the basolateral surface after a 1- to 24-h chase. These data suggest that immature Na+-K+-ATPase alpha-subunit and core glycosylated beta-subunits are not retained in the endoplasmic reticulum of A6 cells and apparently lack sorting signals. Mature Na+-K+-ATPase is targeted to the basolateral surface, suggesting that basolateral targeting of the protein is conformation dependent. C1 VET AFFAIRS MED CTR, PHILADELPHIA, PA 19104 USA. UNIV PENN, DEPT MED, PHILADELPHIA, PA 19104 USA. UNIV PENN, DEPT PHYSIOL, PHILADELPHIA, PA 19104 USA. UNIV MICHIGAN, DEPT ANAT & CELL BIOL, ANN ARBOR, MI 48109 USA. NR 30 TC 11 Z9 12 U1 0 U2 1 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0363-6143 J9 AM J PHYSIOL-CELL PH JI Am. J. Physiol.-Cell Physiol. PD JUN PY 1997 VL 272 IS 6 BP C1781 EP C1789 PG 9 WC Cell Biology; Physiology SC Cell Biology; Physiology GA XF782 UT WOS:A1997XF78200004 ER PT J AU Lloyd, KCK Amirmoazzami, S Friedik, F Chew, P Walsh, JH AF Lloyd, KCK Amirmoazzami, S Friedik, F Chew, P Walsh, JH TI Somatostatin inhibits gastrin release and acid secretion by activating sst(2) in dogs SO AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY LA English DT Article DE somatostatin receptors; stomach; G cells ID FAT-INDUCED INHIBITION; PERFUSED RAT STOMACH; MONOCLONAL-ANTIBODY IMMUNONEUTRALIZATION; ENTEROCHROMAFFIN-LIKE CELLS; HISTAMINE-RELEASE; PRIMARY CULTURE; INTESTINAL FAT; RECEPTOR SUBTYPE; PARIETAL-CELLS; MUCOSAL CELLS AB Somatostatin is a potent inhibitor of gastrin-stimulated acid secretion by activation of somatostatin receptor type 2 (sst(2)) in vivo, probably in part by blocking gastrin-stimulated histamine release from enterochromaffin-like cells expressing sst(2). We propose that activation of sst(2) may also regulate meal-stimulated acid secretion by blocking gastrin release from antral G cells. Using peptide analogs relatively selective for sst(2) (NC-8-12), sst(3) (BIM-23058), and sst(5) (BIM-23052), we tested this hypothesis in two ways: first, in vivo by measuring plasma gastrin release during meal-stimulated acid secretion in dogs, and second, in vitro by measuring bombesin-stimulated gastrin release from an enriched culture of canine antral G cells. In vivo, a low dose (0.05 nmol.kg(-1).h(-1)) of NC-8-12 inhibited acid secretion 56 +/- 16% without blocking gastrin release. A higher dose (1 nmol.kg(-1).h(-1)) of NC-8-12 abolished acid secretion and inhibited gastrin release by 61 +/- 4%, whereas the highest dose (5 nmol.kg(-1).h(-1)) inhibited gastrin release by 84 +/- 3%. Only the highest doses (5 nmol.kg(-1).h(-1)) of BIM-23058 and BIM-23052 significantly inhibited gastrin release and acid secretion. In vitro, NC-8-12 (10(-9) M) reduced bombesin-stimulated gastrin release from antral G cells by 49 +/- 5%, whereas BIM-23058 and BIM-23052 were at least 100-fold less effective. These results indicate that somatostatin activation of sst(2), but not sst(3) Or sst(5), is the major pathway for somatostatin-induced inhibition of meal-stimulated gastrin release and acid secretion. C1 W LOS ANGELES VET AFFAIRS MED CTR, RES SERV, LOS ANGELES, CA 90073 USA. W LOS ANGELES VET AFFAIRS MED CTR, MED SERV, LOS ANGELES, CA 90073 USA. W LOS ANGELES VET AFFAIRS MED CTR, CURE, DIGEST DIS RES CTR, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT MED, LOS ANGELES, CA 90095 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT PHYSIOL, LOS ANGELES, CA 90095 USA. NR 55 TC 24 Z9 25 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0193-1857 J9 AM J PHYSIOL-GASTR L JI Am. J. Physiol.-Gastroint. Liver Physiol. PD JUN PY 1997 VL 272 IS 6 BP G1481 EP G1488 PG 8 WC Gastroenterology & Hepatology; Physiology SC Gastroenterology & Hepatology; Physiology GA XG113 UT WOS:A1997XG11300023 ER PT J AU Kraut, JA Hiura, J Besancon, M Smolka, A Sachs, G Scott, D AF Kraut, JA Hiura, J Besancon, M Smolka, A Sachs, G Scott, D TI Effect of hypokalemia on the abundance of HK alpha(1) and HK alpha(2) protein in the rat kidney SO AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY LA English DT Article; Proceedings Paper CT XIIIth International Congress of Nephrology CY JUL 02-06, 1995 CL MADRID, SPAIN DE renal hydrogen-potassium-adenosinetriphosphatase; potassium; immunoprecipitation; Western blot analysis ID H-K-ATPASE; GASTRIC H,K-ATPASE; DISTAL COLON; ADENOSINE-TRIPHOSPHATASE; FUNCTIONAL EXPRESSION; COLLECTING DUCT; IMMUNOREACTIVITY; TRANSPORT; TUBULE AB An H+-K+-adenosinetriphosphatase (H+-K+-ATPase) contributes to potassium reabsorption by the collecting ducts of the rat kidney. mRNAs for two isoforms of the H+-K+-ATPase, HK alpha(1) and HK alpha(2), have been found in the rat kidney. To evaluate whether the HK alpha(1) and HK alpha(2) proteins are present in the rat kidney, microsomes enriched in HK alpha(1) or HK alpha(2) were isolated using the MiniMac magnetic separation system with antibodies directed against either HK alpha(1) (HK 12.18) or HK alpha(2) (AS 31.7). Immunoblots of rat kidney microsomal protein isolated with HK 12.18 revealed a band similar to 94 kDa in size that comigrated with the G1 fraction of the stomach. Immunoblots of rat kidney microsomal protein isolated with AS 31.7 revealed a band slightly greater than 94 kDa that comigrated with a band obtained from rat colonic microsomal protein. To examine the effect of perturbations in potassium metabolism, the abundance of the HK alpha(1) and HK alpha(2) isoforms was compared in rats fed a normal or potassium-deficient diet. A low-potassium diet increased the abundance of HK alpha(2), whereas that of HK alpha(1) was not altered. These data suggest that HK alpha(2) might be the isoform responsible for potassium conservation by the kidney. C1 UNIV CALIF LOS ANGELES, SCH MED,W LOS ANGELES VET AFFAIRS MED CTR, MEMBRANE BIOL LAB,RES SERV, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, SCH MED, W LOS ANGELES VET AFFAIRS MED CTR, DEPT MED, LOS ANGELES, CA 90073 USA. MED UNIV S CAROLINA, DEPT MED, CHARLESTON, SC 29425 USA. RP Kraut, JA (reprint author), UNIV CALIF LOS ANGELES, SCH MED, W LOS ANGELES VET AFFAIRS MED CTR, DIV NEPHROL 691111L, LOS ANGELES, CA 90073 USA. FU NIDDK NIH HHS [DK-43138] NR 31 TC 41 Z9 42 U1 0 U2 1 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 1931-857X J9 AM J PHYSIOL-RENAL JI Am. J. Physiol.-Renal Physiol. PD JUN PY 1997 VL 272 IS 6 BP F744 EP F750 PG 7 WC Physiology; Urology & Nephrology SC Physiology; Urology & Nephrology GA XG017 UT WOS:A1997XG01700008 PM 9227635 ER PT J AU Green, MF Marshall, BD Wirshing, WC Ames, D Marder, SR McGurk, S Kern, RS Mintz, J AF Green, MF Marshall, BD Wirshing, WC Ames, D Marder, SR McGurk, S Kern, RS Mintz, J TI Does risperidone improve verbal working memory in treatment-resistant schizophrenia? SO AMERICAN JOURNAL OF PSYCHIATRY LA English DT Article ID NEUROPSYCHOLOGICAL TEST-PERFORMANCE; ATTENTIONAL PERFORMANCE; PSYCHIATRIC-SYMPTOMS; COGNITIVE FUNCTIONS; CLOZAPINE; MEDICATION AB Objective: Treatment efficacy in schizophrenia is typically defined in terms of symptom reduction. However, new antipsychotic medications could potentially have an impact on aspects of disability, such as neurocognitive deficits. The authors evaluated the effects of risperidone on vel bal working memory, a memory component of theoretical interest because of its link to prefrontal activity and of practical interest because of its link to psychosocial rehabilitation. Method: Verbal working memory of 59 treatment-resistant schizophrenic patients was assessed as part of a randomized, double-blind comparison of treatment with risperidone and haloperidol. Verbal working memory was measured under both distracting and nondistracting conditions at baseline and after 4 weeks of both fixed- and flexible-dose pharmacotherapy. Results: Risperidone treatment had a greater beneficial effect on verbal working memory than haloperidol treatment across testing conditions (with and without distraction) and study phases (fixed and flexible nose). The treatment effect remained significant after the effects of benztropine cotreatment, change in psychotic symptoms, and change in negative symptoms were controlled. Neither benztropine status nor symptom changes were significantly related to memory Performance. Conclusions: Treatment with risperidone appears to exert a more favorable effect on verbal working memory than treatment with a conventional neuroleptic. The beneficial effect appears to be due, at least partially, to a direct effect of the drug, possibly through antagonism of the 5-HT2A receptor. Results from this study suggest that pharmacotherapeutic efficacy in schizophrenia treatment could be broadened to include impact on neurocognitive abilities. C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. CAMARILLO STATE HOSP & DEV CTR,LOS ANGELES,CA. RP Green, MF (reprint author), UNIV CALIF LOS ANGELES,DEPT PSYCHIAT & BIOBEHAV SCI,760 WESTWOOD PLAZA,C9-420,LOS ANGELES,CA 90024, USA. NR 33 TC 345 Z9 351 U1 3 U2 3 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 0002-953X J9 AM J PSYCHIAT JI Am. J. Psychiat. PD JUN PY 1997 VL 154 IS 6 BP 799 EP 804 PG 6 WC Psychiatry SC Psychiatry GA XA587 UT WOS:A1997XA58700012 PM 9167507 ER PT J AU Glassman, PA Jacobson, PD Asch, S AF Glassman, PA Jacobson, PD Asch, S TI Medical necessity and defined coverage benefits in the Oregon Health Plan SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Article ID RATIONING PLAN; MANAGED CARE; PHYSICIANS; INSURANCE AB The policy debate in Oregon has primarily focused on the Prioritized List of Services. However, little information is available on how defined coverage benefits and managed care affect the role of medical necessity in determining care for Medicaid patients. This issue is important because medical necessity determinations are currently used by many states to limit extraneous health care costs but require resource-intensive oversight, are open to wide variance, and frequently prompt litigation challenging interpretations of what is necessary and what is not. The qualitative study described here addressed whether medical necessity remains a salient and useful concept in the Oregon Health Plan. Our results indicate that defined coverage benefits, as described by the funded portion of the Prioritized List of Services, supplant medical necessity determinations for coverage, while managed care incentives Limit the need for medical necessity determinations at the provider level. Clinical choices are, for the most part, guided by providers' judgment within the financial constraints of capitation and by targeted use management techniques. The combination of capitated care and Oregon's defined coverage benefits package has marginalized the use of medical necessity, albeit with consequences for state oversight of Medicaid services. C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. RAND CORP,SANTA MONICA,CA. UNIV MICHIGAN,SCH PUBL HLTH,DEPT HLTH POLICY & MANAGEMENT,ANN ARBOR,MI 48109. UNIV SO CALIF,LOS ANGELES,CA. RP Glassman, PA (reprint author), UNIV CALIF LOS ANGELES,DIV GEN INTERNAL MED 111G,11301 WILSHIRE AVE,LOS ANGELES,CA 90073, USA. NR 23 TC 3 Z9 3 U1 0 U2 1 PU AMER PUBLIC HEALTH ASSOC INC PI WASHINGTON PA 1015 FIFTEENTH ST NW, WASHINGTON, DC 20005 SN 0090-0036 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD JUN PY 1997 VL 87 IS 6 BP 1053 EP 1058 DI 10.2105/AJPH.87.6.1053 PG 6 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA XJ859 UT WOS:A1997XJ85900039 PM 9224198 ER PT J AU Eubanks, PJ Sawicki, MP Samara, GJ Wan, YJY Gatti, RA Hurwitz, M Passaro, E AF Eubanks, PJ Sawicki, MP Samara, GJ Wan, YJY Gatti, RA Hurwitz, M Passaro, E TI Pancreatic endocrine tumors with loss of heterozygosity at the multiple endocrine neoplasia type I locus SO AMERICAN JOURNAL OF SURGERY LA English DT Article ID SUPPRESSOR GENE; CHROMOSOME-11 AB BACKGROUND: Loss of heterozygosity (LOH) at chromosome 11q13 has been demonstrated in multiple endocrine neoplasia type I (MEN I) and sporadic parathyroid tumors, pituitary adenomas, and a few types of pancreatic endocrine tumors. Gastrinomas are the most common pancreatic endocrine tumor in MEN I. We hypothesized that all pancreatic endocrine tumors have LOH at 11q13, resulting in inactivation of the previously described tumor suppressor gene in this region. METHODS: We analyzed a sporadic gastrinoma, a MEN I-associated gastrinoma, and a nonfunctional pancreatic endocrine tumor: from a patient with Von Hippel-Lindau (VHL) disease for LOH at seven loci at 11q13: D11S149, PYGM, D11S427, D11S546, SEA, D11S97, and D11S146. RESULTS AND CONCLUSIONS: We found LOH at 11q13 in all three tumors. The MEN I-associated gastrinoma we analyzed is the first tumor of this type to have LOH. This is also the first report of LOH at 11q13 in a pancreatic endocrine tumor from a patient with VHL. These findings suggest that the etiology of pancreatic endocrine tumor formation involves a common genetic pathway for sporadic, MEN I, and VHL tumors. (C) 1997 by Excerpta Medica, Inc. C1 UNIV CALIF LOS ANGELES,HARBOR MED CTR,DEPT PATHOL,TORRANCE,CA 90509. UNIV CALIF LOS ANGELES,SCH MED,DEPT SURG,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,DEPT PATHOL,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. RP Eubanks, PJ (reprint author), UNIV CALIF LOS ANGELES,HARBOR MED CTR,DEPT SURG,1000 W CARSON ST,BOX 15,TORRANCE,CA 90509, USA. NR 10 TC 8 Z9 8 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9610 J9 AM J SURG JI Am. J. Surg. PD JUN PY 1997 VL 173 IS 6 BP 518 EP 520 DI 10.1016/S0002-9610(97)00001-9 PG 3 WC Surgery SC Surgery GA XG218 UT WOS:A1997XG21800013 PM 9207166 ER PT J AU Grover, FL Fullerton, DA Zamora, MR Mills, C Ackerman, B Badesch, D Brown, JM Campbell, DN Chetham, P Dhaliwal, A Diercks, M Kinnard, T Niejadlik, K Ochs, M AF Grover, FL Fullerton, DA Zamora, MR Mills, C Ackerman, B Badesch, D Brown, JM Campbell, DN Chetham, P Dhaliwal, A Diercks, M Kinnard, T Niejadlik, K Ochs, M TI The past, present, and future of lung transplantation SO AMERICAN JOURNAL OF SURGERY LA English DT Article; Proceedings Paper CT 47th Annual Meeting of the Southwestern Surgical Conference CY APR 23-26, 1995 CL SAN ANTONIO, TX ID OBLITERATIVE BRONCHIOLITIS; LOBAR TRANSPLANTATION; AUTO-TRANSPLANTATION; CYSTIC-FIBROSIS; HEART; METHYLPREDNISOLONE; AZATHIOPRINE; RECIPIENTS; EMPHYSEMA; SURVIVAL AB BACKGROUND: The history of lung transplantation from the first human transplant performed in 1963 to the present is reviewed with particular focus on the added challenges because of the contaminated bronchus, exposure of the graft to airborne organisms, the poor blood supply to the bronchus, and the problem of reperfusion pulmonary edema. METHODS: The technical aspects of single and double sequential lung transplantation are reviewed, as are the current indications for single, double sequential, and heart/lung transplantation. Criteria for lung transplant recipients, in addition to their primary disease are noted, as are absolute and relative contraindications. The standard criteria for donor selection are also reviewed. RESULTS: The results of single, double sequential, and heart-lung transplantation over the past 10 years as reported by the International Society for Heart and Lung Transplantation Database are reviewed. In addition, the statistics of the lung and heart-lung transplantation program at the University of Colorado Health Sciences Center are reviewed, including the current immunosuppressive regimens and early and late monitoring for infection and rejection. This experience includes 3 early deaths in the first 53 patients for an operative mortality of 5.6%, with a 1-year actuarial survival of 90%. CONCLUSIONS: During the past decade remarkable improvement in the result of single and double sequential lung transplantation have occurred. As 1-year, actuarial survival is now approaching 90% at some institutions. Living related lobar transplantation, new antirejection agents, chimerism, and xenograft transplantation are areas for continuing and future investigation. The shortage in donor organ supply continues to be a very significant factor in limiting human lung transplantation. (C) 1997 by Excerpta Medica, Inc. C1 UNIV COLORADO,HLTH SCI CTR,DIV PULM MED,DENVER,CO 80262. UNIV COLORADO,HLTH SCI CTR,DEPT ANESTHESIOL,DENVER,CO 80262. DENVER VA MED CTR,DENVER,CO. RP Grover, FL (reprint author), UNIV COLORADO,HLTH SCI CTR,DIV CARDIOTHORAC SURG C310,4200 E 9TH AVE,DENVER,CO 80262, USA. NR 31 TC 22 Z9 22 U1 0 U2 1 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9610 J9 AM J SURG JI Am. J. Surg. PD JUN PY 1997 VL 173 IS 6 BP 523 EP 533 DI 10.1016/S0002-9610(97)00004-4 PG 11 WC Surgery SC Surgery GA XG218 UT WOS:A1997XG21800015 PM 9207168 ER PT J AU Sivarajan, M Wasse, L AF Sivarajan, M Wasse, L TI Perioperative acute renal failure associated with preoperative intake of ibuprofen SO ANESTHESIOLOGY LA English DT Article DE nonsteroidal antiinflammatory drugs; renal dysfunction, perioperative ID NONSTEROIDAL ANTIINFLAMMATORY DRUGS; KETOROLAC C1 UNIV WASHINGTON,SCH MED,DEPT ANESTHESIOL,SEATTLE,WA 98195. RP Sivarajan, M (reprint author), VET AFFAIRS PUGET SOUND HLTH CARE SYST,SEATTLE DIV,DEPT ANESTHESIOL 112A,ANESTHESIOL SERV,SEATTLE,WA 98108, USA. NR 10 TC 13 Z9 13 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0003-3022 J9 ANESTHESIOLOGY JI Anesthesiology PD JUN PY 1997 VL 86 IS 6 BP 1390 EP 1392 DI 10.1097/00000542-199706000-00023 PG 3 WC Anesthesiology SC Anesthesiology GA XE806 UT WOS:A1997XE80600023 PM 9197311 ER PT J AU Badgett, RG OKeefe, M Henderson, MC AF Badgett, RG OKeefe, M Henderson, MC TI Using systematic reviews in clinical education SO ANNALS OF INTERNAL MEDICINE LA English DT Article ID MEDICAL LITERATURE; ALCOHOLIC HEPATITIS; INFORMATION NEEDS; METAANALYSIS; PHYSICIANS; KNOWLEDGE; SCIENCE; TRIALS; CARE AB Traditional educational methods change clinical practice only with considerable effort and difficulty. In particular, the teaching of critical appraisal in the setting of journal clubs does not increase the amount of medical research read by trainees. Experiential learning theory, corroborated by the success of problem-based learning, encourages us to link learning to the numerous medical questions that physicians generate while providing patient care. Systematic reviews can link these questions with the results of research that would otherwise be difficult to locate, read, and appraise. Systematic reviews are a uniquely powerful mechanism for teaching, and they offer teachers a new opportunity to model rational and effective use of information. Systematic reviews should be made available at clinical sites for use during ''teachable moments.'' Resistance to the use of systematic reviews can be reduced by using existing journal clubs to teach about the strengths and limitations of these reviews. The point that systematic reviews are meant to assist, not replace, clinical decision making deserves emphasis in such teaching. C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RP Badgett, RG (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT MED,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. OI Badgett, Robert/0000-0003-2888-3303 NR 46 TC 19 Z9 21 U1 0 U2 1 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD JUN 1 PY 1997 VL 126 IS 11 BP 886 EP 891 PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA XA911 UT WOS:A1997XA91100006 PM 9163290 ER PT J AU Freedman, LR AF Freedman, LR TI Helping physicians recognize bedside rationing SO ANNALS OF INTERNAL MEDICINE LA English DT Letter RP Freedman, LR (reprint author), UNIV CALIF LOS ANGELES,SCH MED,W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD JUN 1 PY 1997 VL 126 IS 11 BP 921 EP 921 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA XA911 UT WOS:A1997XA91100030 PM 9163308 ER PT J AU Small, JG Hirsch, SR Arvanitis, LA Miller, BG Link, CGG Murphy, AL Bowden, CL OBrien, CP Steinbook, RM Wagner, RL Pitts, WM Crabtree, B Corrigan, MHN Lohr, JB Miller, AL Ereshefsky, L Anderson, CB Knesevich, MA Rotrosen, J Owen, RR Karson, CN Lindenmayer, JP Pfefferbaum, A Faustman, WO Keck, PE Patterson, WM Riesenberg, RA Gladson, MB Kolin, IS Barnes, TRE Sensky, T Riccio, M Franks, S Jolley, A Hallstrom, C Bennie, E Darcourt, G Patris, M DeSilva, M Patel, AG Fleischhacker, WW Dietzel, M Seifertova, D Vinar, O Faltus, F Bitter, I Ozsvath, K Shaw, SH Mahapatra, SN Szulecka, TK Sundararajan, K Creaven, F Lynch, J Klieser, E Cooper, SJ Aschauer, H AF Small, JG Hirsch, SR Arvanitis, LA Miller, BG Link, CGG Murphy, AL Bowden, CL OBrien, CP Steinbook, RM Wagner, RL Pitts, WM Crabtree, B Corrigan, MHN Lohr, JB Miller, AL Ereshefsky, L Anderson, CB Knesevich, MA Rotrosen, J Owen, RR Karson, CN Lindenmayer, JP Pfefferbaum, A Faustman, WO Keck, PE Patterson, WM Riesenberg, RA Gladson, MB Kolin, IS Barnes, TRE Sensky, T Riccio, M Franks, S Jolley, A Hallstrom, C Bennie, E Darcourt, G Patris, M DeSilva, M Patel, AG Fleischhacker, WW Dietzel, M Seifertova, D Vinar, O Faltus, F Bitter, I Ozsvath, K Shaw, SH Mahapatra, SN Szulecka, TK Sundararajan, K Creaven, F Lynch, J Klieser, E Cooper, SJ Aschauer, H TI Quetiapine in patients with schizophrenia - A high- and low-dose double-blind comparison with placebo SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Article ID RISPERIDONE AB Background: Quetiapine fumarate (Seroquel [ICI 204,636]) is an atypical dibenzothiazepine antipsychotic with a greater affinity for 5-hydroxytryptamine(2) (5-HT2) receptors than for D-2 dopamine receptors; its efficacy in patients with schizophrenia was shown in early phase 2 trials (maximum dose, 750 mg/d). Methods: In this multicenter, double-blind, placebo-controlled trial, 286 patients hospitalized with chronic or subchronic schizophrenia (DSM-III-R) were randomized to 6 weeks of treatment with high-dose quetiapine fumarate (less than or equal to 750 mg/d), n = 96; low-dose quetiapine fumarate (less than or equal to 250 mg/d), n = 94; or placebo, n = 96. The Brief Psychiatric Rating Scale (BPRS) and Clinical Global Impression Severity of Illness item scores were the primary efficacy variables. Secondary efficacy variables included the BPRS positive-symptom cluster score, the Modified Scale for the Assessment of Negative Symptoms summary score (United States only), and the total score from the negative scale of the Positive and Negative Syndrome Scale (Europe only). Scores were analyzed using an analysis of covariance for change from baseline at end point with last observations carried forward. The model included baseline score (covariate), center, and treatment. Extrapyramidal symptoms were assessed using the Simpson-Angus Scale and the Barnes Akathisia Scale; abnormal involuntary movements were assessed using the Abnormal Involuntary Movement Scale. Frequency distributions of grouped change-from-baseline scores were analyzed using chi(2) tests. Results: Of 280 patients in whom the efficacy of quetiapine was evaluated, 159 (42% of those receiving high-dose treatment; 57%, low-dose treatment; and 59%, placebo) withdrew before trial completion, primarily because of treatment failure. Significant (P < .001, BPRS; P = .003, Clinical Global Impression Severity of Illness item; and P = .003, BPRS positive-symptom cluster) differences were identified between patients receiving high-dose quetiapine and placebo for both primary efficacy variables, with end point differences in the BPRS positive-symptom cluster score showing quetiapine's consistency in reducing positive symptoms. The reduction of negative symptoms was less consistent; high-dose quetiapine was superior on the Modified Scale for the Assessment of Negative Symptoms but not on the negative scale of the Positive and Negative Syndrome Scale. Quetiapine was well tolerated and did not induce extrapyramidal symptoms, sustained elevations of prolactin, or clinically significant changes in hematologic parameters. Conclusions: Quetiapine is an effective antipsychotic with a favorable safety profile. The optimum dose is probably greater than 250 mg/d. C1 LARUE D CARTER MEM HOSP, DIV MENTAL HLTH, INDIANAPOLIS, IN USA. CHARING CROSS HOSP, DEPT PSYCHIAT, LONDON, ENGLAND. ZENECA PHARMACEUT, DEPT CLIN & MED AFFAIRS, WILMINGTON, DE USA. ZENECA PHARMACEUT, DEPT MED AFFAIRS, MACCLESFIELD, CHESHIRE, ENGLAND. VET MEM HOSP, SAN ANTONIO, TX USA. VET AFFAIRS MED CTR, PHILADELPHIA, PA USA. UNIV MIAMI, JACKSON MEM MED CTR, MIAMI, FL 33101 USA. INST MENTAL HLTH, CRANSTON, RI USA. UNIV MISSISSIPPI, MED CTR, JACKSON, MS 39216 USA. VET AFFAIRS MED CTR, JACKSON, MS USA. DOROTHEA DIX HOSP, RALEIGH, NC USA. VET AFFAIRS MED CTR, SAN DIEGO, CA 92161 USA. UNIV TEXAS, HLTH SCI CTR, SAN ANTONIO, TX USA. SAN ANTONIO STATE HOSP, SAN ANGELO, TX USA. CLIN PHARM PROGRAMS, SAN ANTONIO, TX USA. TERRELL STATE HOSP, TERRELL, TX USA. NEW YORK VET AFFAIRS MED CTR, NEW YORK, NY USA. VET AFFAIRS MED CTR, LITTLE ROCK, AR USA. ARKANSAS STATE HOSP, LITTLE ROCK, AR USA. SCHIZOPHREN RES UNIT, BRONX, NY USA. STANFORD VET AFFAIRS MENT HLTH CLIN RES CTR, PALO ALTO, CA USA. UNIV CINCINNATI, COLL MED, CINCINNATI, OH USA. HILLCREST HOSP, BIRMINGHAM RES GRP, BIRMINGHAM, AL USA. DEKALB MED CTR, DECATUR, GA USA. SAND LAKE HOSP, ORLANDO, FL USA. HORTON HOSP, SURREY, ENGLAND. W MIDDLESEX HOSP, ISLEWORTH, MIDDX, ENGLAND. ST MARY ABBOT HOSP, LONDON, ENGLAND. GORDON HOSP, LONDON, ENGLAND. EALING GEN HOSP, LONDON, MIDDX, ENGLAND. CHARING CROSS HOSP, LONDON, ENGLAND. LEVERNADALE HOSP, GLASGOW, LANARK, SCOTLAND. PASTEUR HOSP, CLIN PSYCHIAT & PSYCOL, NICE, FRANCE. PSYCHIAT CLIN, STRASBOURG, FRANCE. UNIV INNSBRUCK CLIN, INNSBRUCK, AUSTRIA. UNIV VIENNA, PSYCHIAT CLIN, A-1090 VIENNA, AUSTRIA. INST PSYCHIAT RES, PRAGUE, CZECH REPUBLIC. HOSP PSYCHIAT, PRAGUE, CZECH REPUBLIC. CHARLES UNIV, PRAGUE, CZECH REPUBLIC. SEMMELWEIS UNIV MED, H-1085 BUDAPEST, HUNGARY. UNIV PECS, SCH MED, PECS, HUNGARY. GEN HOSP, HARTLEPOOL, ENGLAND. BASSETLAW DISTRICT GEN HOSP, NOTTINGHAM, ENGLAND. HALIFAX GEN HOSP, HALIFAX, W YORKSHIRE, ENGLAND. ST MARYS PSYCHIAT HOSP, CASLEBAR, MAYO, IRELAND. ST LUKES HOSP, CLONMEL, TIPPERARY, IRELAND. PSYCHIAT KLIN, DUSSELDORF, GERMANY. QUEENS UNIV BELFAST, BELFAST, ANTRIM, NORTH IRELAND. UNIV CLIN VIENNA, VIENNA, AUSTRIA. NR 20 TC 430 Z9 455 U1 0 U2 9 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60654-0946 USA SN 0003-990X J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD JUN PY 1997 VL 54 IS 6 BP 549 EP 557 PG 9 WC Psychiatry SC Psychiatry GA XE945 UT WOS:A1997XE94500007 PM 9193196 ER PT J AU Gabriel, SM Haroutunian, V Powchik, P Honer, WG Davidson, M Davies, P Davis, KL AF Gabriel, SM Haroutunian, V Powchik, P Honer, WG Davidson, M Davies, P Davis, KL TI Increased concentrations of presynaptic proteins in the cingulate cortex of subjects with schizophrenia SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Article ID SYNAPTOSOMAL-ASSOCIATED PROTEIN; ALZHEIMERS-DISEASE; COGNITIVE IMPAIRMENT; PREFRONTAL CORTEX; SYNAPTIC VESICLES; CEREBRAL-CORTEX; RAT-BRAIN; SYNAPTOPHYSIN; HALOPERIDOL; EXPRESSION AB Background: Cytoarchitectural and neurochemical studies demonstrate disorganization in the cerebral cortex in schizophrenia, which perhaps underlies the severe behavioral disturbances of the disease. This neuronal disarray should be accompanied by synaptic abnormalities. As such, presynaptic proteins have proved valuable indexes of synaptic density and their concentrations have correlated markedly with synaptic loss. Our study sought to determine whether abnormalities exist in the concentrations of presynaptic proteins in the postmortem cerebral cortex of subjects with schizophrenia. Methods: Presynaptic protein immunoreactivities were assessed in 4 different cerebrocortical regions derived from 16 elderly controls, 19 elderly subjects with schizophrenia, and 24 subjects with Alzheimer's disease. Tissues were assayed with the monoclonal antibodies EP10 and SP4, which recognize synaptophysin, and the monoclonal antibodies SP6 and SP14, which detect syntaxin and synaptosomal-associated protein-25-kd immunoreactivities, respectively. Results: In subjects with schizophrenia relative to controls, presynaptic proteins were increased in the cingulate cortex, but were unchanged in the temporal, frontal, and parietal cortices. In contrast, when cases with Alzheimer's disease were compared with controls, pre synaptic proteins were decreased in the frontal, temporal, and parietal samples. Conclusions: These findings reveal changes in the synaptic organization of the cingulate cortex in schizophrenia relative to other areas examined. These changes are distinct from the deficits in presynaptic proteins observed in Alzheimer's disease. C1 BRONX VET AFFAIRS MED CTR, DEPT PSYCHIAT, BRONX, NY 10468 USA. MT SINAI SCH MED, DEPT PSYCHIAT, NEW YORK, NY USA. ALBERT EINSTEIN COLL MED, DEPT PATHOL, BRONX, NY 10467 USA. UNIV BRITISH COLUMBIA, DEPT PSYCHIAT, VANCOUVER, BC, CANADA. FU NIA NIH HHS [AG02219, AG5138]; NIMH NIH HHS [MH46436] NR 52 TC 105 Z9 106 U1 1 U2 1 PU AMER MEDICAL ASSOC PI CHICAGO PA 330 N WABASH AVE, STE 39300, CHICAGO, IL 60611-5885 USA SN 0003-990X EI 1538-3636 J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD JUN PY 1997 VL 54 IS 6 BP 559 EP 566 PG 8 WC Psychiatry SC Psychiatry GA XE945 UT WOS:A1997XE94500008 PM 9193197 ER PT J AU Aiba, Y Hirayama, F Ogawa, M AF Aiba, Y Hirayama, F Ogawa, M TI Clonal proliferation and cytokine requirement of murine progenitors for natural killer cells SO BLOOD LA English DT Article ID RECEPTOR-GAMMA-CHAIN; IL-2 RECEPTOR; INTERLEUKIN-2 RECEPTOR; NK CELLS; LYMPHOID DIFFERENTIATION; FUNCTIONAL COMPONENT; MONOCLONAL-ANTIBODY; STAT PROTEINS; BETA-CHAIN; STEM-CELLS AB We have established a clonal cell culture system that supports the proliferation of committed natural killer (NK) cell progenitors of mice to investigate the pathway and cytokine regulation of NK cell development. Day 14 fetal thymocytes cultured in methylcellulose with interleukin-7 (IL-7), IL-15, and steel factor (SF) formed diffuse colonies that could not be classified to known colony types, Single-cell origin of the colonies was established by micromanipulation of the colony-forming cells, Cells in the colonies are very blastic, showing no cytoplasmic differentiation, and express Ly5, Thy-1, and CD25 but not myeloid, B, mature T, or NK cell markers. The cells lack T, B, and myeloid potentials but can differentiate to mature NK cells in fetal thymus organ culture, suggesting that the colonies consist of NK committed progenitors. Examination of the minimal cytokine requirement for the NK colony formation showed that IL-7 and SF are indispensable for the formation of immature NK cell colonies. Both IL-2 and IL-15 increased the frequency of colonies. In contrast to IL-2, IL-7, and IL-15, IL-4 strongly inhibited the formation of the colonies, This quantitative clonal culture will provide a useful means to examine the mechanism of NK cell development. (C) 1997 by The American Society of Hematology. C1 RALPH H JOHNSON DEPT VET AFFAIRS MED CTR, CHARLESTON, SC 29401 USA. MED UNIV S CAROLINA, DEPT MED, CHARLESTON, SC 29425 USA. FU NIDDK NIH HHS [DK/HL48714, DK32294] NR 55 TC 18 Z9 18 U1 0 U2 0 PU AMER SOC HEMATOLOGY PI WASHINGTON PA 1900 M STREET. NW SUITE 200, WASHINGTON, DC 20036 USA SN 0006-4971 J9 BLOOD JI Blood PD JUN 1 PY 1997 VL 89 IS 11 BP 4005 EP 4012 PG 8 WC Hematology SC Hematology GA XD379 UT WOS:A1997XD37900014 PM 9166839 ER PT J AU Jeffers, L Church, D Basu, H Marton, L Wilding, G AF Jeffers, L Church, D Basu, H Marton, L Wilding, G TI Effects of the polyamine analogues BE-4-4-4-4, BE-3-7-3, and BE-3-3-3 on the proliferation of three prostate cancer cell lines SO CANCER CHEMOTHERAPY AND PHARMACOLOGY LA English DT Article DE polyamine; prostate carcinoma; nude mouse ID SPERMIDINE SPERMINE N1-ACETYLTRANSFERASE; ALPHA-DIFLUOROMETHYLORNITHINE; ANTITUMOR-ACTIVITY; NECK TUMORS; GROWTH; DNA; CARCINOMA; INVIVO; POTENTIATION; DEPRIVATION AB Purpose: Polyamines are biologic cations necessary for normal cell growth. Polyamine analogues have been shown to be effective inhibitors of tumor growth. We tested the effect of the polyamine analogues 1,19-bis(ethylamino)-5,10,15-triazanonadecane (BE-4-4-4-4), N-1,N-11-bis(ethyl)norspermine (BE-3-3-3) and 1,15-bis(ethylamino)-4,12-diazapentadecane (BE-3-7-3) on the growth of the prostate cancer cell lines DU145, LNCaP and PC-3 in vitro. We also tested the effect of BE-4-4-4-4 on androgen-independent DU145 cells in vivo via a nude mouse xenograft model. Methods: In vitro, cell proliferation was measured using a DNA assay or a colony-formation assay. In vivo, mice were given saline or BE-4-4-4-4 3 mg/kg or 5 mg/kg intraperitoneally twice daily on days 7-10 and 14-17 (cycle 1), days 49-52 and 56-59 (cycle 2) and days 91-94 and 98-101 (cycle 3). Results: The proliferation of DU145, LNCaP and PC-3 prostate cancer cell lines was inhibited in a dose-dependent manner by BE-4-4-4-4. Intracellular putrescine, spermidine and spermine levels in all three cell lines declined after only 24 h exposure to BE-4-4-4-4 in vitro. Animals receiving BE-4-4-4-4 showed inhibition of tumor growth which continued throughout the experiment with 74% (3 mg/kg) and 81% (5 mg/kg) growth inhibition seen on day 101, No overt toxic reactions besides weight loss were observed in BE-4-4-4-4-treated animals. Tumor tissue from animals treated with BE-4-4-4-4 showed a dose-dependent decrease in spermidine and spermine levels but no decline in putrescine levels as compared with control. BE-4-4-4-4 levels were highest in tumors on day 63 with levels reaching 0.33 and 1.45 nmol/mg protein from animals treated at the 3 mg/kg and 5 mg/kg doses, respectively. Conclusion: These results show the polyamine analogues BE-4-4-4-4, BE-3-3-3 and BE-3-7-3 to be effective inhibitors of prostate cancer cell growth in vitro and BE-4-4-4-4 to be an effective inhibitor of DU145 cells in vivo with minimal toxicity. C1 UNIV WISCONSIN,CTR COMPREHENS CANC,DEPT MED,MADISON,WI 53792. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,NJ. UNIV WISCONSIN,CTR COMPREHENS CANC,DEPT HUMAN ONCOL,MADISON,WI 53792. UNIV WISCONSIN,SCH MED,DEPT PATHOL & LAB MED,MADISON,WI 53792. UNIV WISCONSIN,SCH MED,MCARDLE LAB CANC RES,DEPT ONCOL,MADISON,WI 53706. NR 38 TC 18 Z9 19 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0344-5704 J9 CANCER CHEMOTH PHARM JI Cancer Chemother. Pharmacol. PD JUN PY 1997 VL 40 IS 2 BP 172 EP 179 DI 10.1007/s002800050643 PG 8 WC Oncology; Pharmacology & Pharmacy SC Oncology; Pharmacology & Pharmacy GA XB021 UT WOS:A1997XB02100011 PM 9182840 ER PT J AU DeNino, LA Lawrence, VA Averyt, EC Hilsenbeck, SG Dhanda, R Page, CP AF DeNino, LA Lawrence, VA Averyt, EC Hilsenbeck, SG Dhanda, R Page, CP TI Preoperative spirometry and laparotomy - Blowing away dollars SO CHEST LA English DT Article DE economics; preoperative care; pulmonary complications; pulmonary function testing; surgery/operative ID OBSTRUCTIVE PULMONARY-DISEASE; COMPLICATIONS AB Study Objective: Increasing evidence indicates that routine preoperative diagnostic spirometry (pulmonary function tests [PFTs]) before elective abdominal surgery does not predict individual risk of postoperative pulmonary complications and is overutilized, This economic evaluation estimates potential savings from reduced use of preoperative PFTs. Design: Analyses of (1) real costs (resource consumption to perform tests) and (2) reimbursements (expenditures for charges) by third-party payers. Setting: University-affiliated public and Veterans Affairs hospitals. Patients: Adults undergoing elective abdominal operations. Measurements and results: Average real cost of PFTs Was $19.07 (95% confidence interval [CI], $18.53 to $19.61), based on a time and motion study. Average reimbursement expenditure by third-party payers for PFTs was $85 (range, $33 to $150; 95% CI, $68 to $103), based on Medicare payment of $52 and a survey of nine urban US hospitals with a spectrum of bed sizes and teaching status, Estimates from published literature included the following: (1) annual number of major abdominal operations, 3.5 million; and (2) proportion of PFTs not meeting current guidelines, 39% (95% CI, 0.31 to 0.47). Local data were used when estimates were not available in the literature: (1) proportion of laparotomies that are elective, 76% (95% CI, 0.73 to 0.79); and (2) frequency of PFTs before laparotomy, 69% (95% CI, 0.54 to 0.84), Estimated annual national real costs for preoperative PFTs are $25 million to $45 million, If use of PFTs were reduced by our estimate for the proportion of PFTs not meeting current guidelines, potential annual national cost savings would be $7,925,411 to $21,406,707. National reimbursement expenditures by third-party payers range from more than $90 million to more than $235 million. If use were reduced potential annual savings in reimbursements would be $29,084,076 to $111,345,440. Potential savings to Medicare approach $8 million to $20 million annually. Conclusion: Reduced use of PFTs before elective abdominal surgery could generate substantial savings, Current evidence indicates reduced use would not compromise patients' outcomes. C1 S TEXAS VET HEALTHCARE SYST,AUDIE L MURPHY DIV,DIV GEN MED,SAN ANTONIO,TX. S TEXAS VET HEALTHCARE SYST,AUDIE L MURPHY DIV,SURG SERV,SAN ANTONIO,TX. UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV GEN MED,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV ONCOL,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV GERIATR,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT SURG,SAN ANTONIO,TX 78284. RP DeNino, LA (reprint author), S TEXAS VET HEALTHCARE SYST,AUDIE L MURPHY DIV,CTR GERIATR RES EDUC & CLIN,SAN ANTONIO,TX, USA. NR 17 TC 20 Z9 19 U1 0 U2 0 PU AMER COLL CHEST PHYSICIANS PI NORTHBROOK PA 3300 DUNDEE ROAD, NORTHBROOK, IL 60062-2348 SN 0012-3692 J9 CHEST JI Chest PD JUN PY 1997 VL 111 IS 6 BP 1536 EP 1541 DI 10.1378/chest.111.6.1536 PG 6 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA XC902 UT WOS:A1997XC90200017 PM 9187170 ER PT J AU Susanto, I Peters, JI AF Susanto, I Peters, JI TI Acute lupus pneumonitis with normal chest radiograph SO CHEST LA English DT Article DE corticosteroids; hypoxemia; lupus; pneumonitis ID ERYTHEMATOSUS; DISEASE; HYPOXEMIA; ADHESION AB Patients with acute lupus pneumonitis (ALP) usually have hypoxemia, patchy infiltrates evidenced on a chest x-ray film, and an incomplete response to corticosteroids with high mortality. In contrast, lupus patients with a syndrome of acute reversible hypoxemia (SARH) have hypoxemia with normal chest x-ray films and a rapid response to corticosteroids. We present a case of biopsy-proven ALP with normal initial chest x-ray films, and a normal CT scan. We hypothesize that a continuum of vascular and parenchymal abnormalities may exist in the lungs of lupus patients. This case also illustrates the insensitivity of routine chest radiographs in demonstrating mild or early pneumonitis. C1 AUDIE L MURPHY MEM VET HOSP DIV,S TEXAS VET HLTH CARE SYST,SAN ANTONIO,TX. RP Susanto, I (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV PULM DIS CRIT CARE MED,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. NR 9 TC 15 Z9 15 U1 0 U2 0 PU AMER COLL CHEST PHYSICIANS PI NORTHBROOK PA 3300 DUNDEE ROAD, NORTHBROOK, IL 60062-2348 SN 0012-3692 J9 CHEST JI Chest PD JUN PY 1997 VL 111 IS 6 BP 1781 EP 1783 DI 10.1378/chest.111.6.1781 PG 3 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA XC902 UT WOS:A1997XC90200061 PM 9187214 ER PT J AU Anderson, PH Bankson, DD Bock, JL Bodin, SG Eisen, CN Levine, JB Marcus, MN Senior, MB Zhou, Z AF Anderson, PH Bankson, DD Bock, JL Bodin, SG Eisen, CN Levine, JB Marcus, MN Senior, MB Zhou, Z TI Multi-site evaluation of PSA in free and ACT-complexed standard mixtures, CAP certified reference serum, survey samples and human serum. SO CLINICAL CHEMISTRY LA English DT Meeting Abstract C1 ST VINCENTS MED CTR,DEPT PATHOL,PORTLAND,OR. VA PUGET SND HLTH CARE,PATHOL & LAB MED SERV,SEATTLE,WA. SUNY STONY BROOK,MED CTR,STONY BROOK,NY 11794. HUNTSVILLE DIST MEM HOSP,DEPT CHEM,HUNTSVILLE,AL. MEM SLOAN KETTERING CANC CTR,DEPT CLIN CHEM,NEW YORK,NY 10021. BAYER DIAGNOST,CLIN & SCI SVC,TARRYTOWN,NY. HOSP UNIV PENN,SCH MED,DEPT ENDOCRINOL,PHILADELPHIA,PA 19104. BAYER DIAGNOST,ADV TECHNOL,TARRYTOWN,NY. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CLINICAL CHEMISTRY PI WASHINGTON PA 2101 L STREET NW, SUITE 202, WASHINGTON, DC 20037-1526 SN 0009-9147 J9 CLIN CHEM JI Clin. Chem. PD JUN PY 1997 VL 43 SU 6 BP 140 EP 140 PN 2 PG 1 WC Medical Laboratory Technology SC Medical Laboratory Technology GA XD363 UT WOS:A1997XD36300140 ER PT J AU Hershman, JM Berg, L AF Hershman, JM Berg, L TI Rapid qualitative TSH test to screen for primary hypothyroidism SO CLINICAL CHEMISTRY LA English DT Letter C1 UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA 90073. RP Hershman, JM (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,ENDOCRINE RES LAB,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 2 TC 3 Z9 3 U1 0 U2 0 PU AMER ASSOC CLINICAL CHEMISTRY PI WASHINGTON PA 2101 L STREET NW, SUITE 202, WASHINGTON, DC 20037-1526 SN 0009-9147 J9 CLIN CHEM JI Clin. Chem. PD JUN PY 1997 VL 43 IS 6 BP 1097 EP 1098 PN 1 PG 2 WC Medical Laboratory Technology SC Medical Laboratory Technology GA XC898 UT WOS:A1997XC89800041 PM 9191577 ER PT J AU Johnson, CC Baldessarre, J Levison, ME AF Johnson, CC Baldessarre, J Levison, ME TI Peritonitis: Update on pathophysiology, clinical manifestations, and management SO CLINICAL INFECTIOUS DISEASES LA English DT Article ID SPONTANEOUS BACTERIAL PERITONITIS; NECROSIS-FACTOR-ALPHA; INTRAABDOMINAL INFECTION; ASCITIC FLUID; FUNGAL PERITONITIS; TNF-ALPHA; ANTIBIOTIC-THERAPY; MYCOBACTERIAL PERITONITIS; DIFFUSE PERITONITIS; SURGICAL PATIENTS C1 ALLEGHENY UNIV HLTH SCI,MCP,DIV INFECT DIS,PHILADELPHIA,PA 19129. RW JOHNSON PHARMACEUT RES INST,RARITAN,NJ 08869. VET AFFAIRS MED CTR,PHILADELPHIA,PA. NR 98 TC 56 Z9 58 U1 0 U2 2 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD JUN PY 1997 VL 24 IS 6 BP 1035 EP 1045 DI 10.1086/513658 PG 11 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA XE366 UT WOS:A1997XE36600001 PM 9195055 ER PT J AU Sekeres, MA Abrutyn, E Berlin, JA Kaye, D Kinman, JL Korzeniowski, OM Levison, ME Feldman, RS Strom, BL AF Sekeres, MA Abrutyn, E Berlin, JA Kaye, D Kinman, JL Korzeniowski, OM Levison, ME Feldman, RS Strom, BL TI An assessment of the usefulness of the Duke criteria for diagnosing active infective endocarditis SO CLINICAL INFECTIOUS DISEASES LA English DT Article AB We evaluated the usefulness of the Duke criteria for diagnosing cases of active infective endocarditis (IE). Patients were identified prospectively over a 3-year period at 54 hospitals in the Philadelphia metropolitan area. Three of us independently reviewed abstracted hospital records and classified 410 patients as definite, probable, or possible cases of IE or as probable noncases, We then applied the Duke criteria to this sample to assess the degree of agreement between our diagnoses and the diagnoses based on these new criteria. Agreement was good to excellent, ranging from 72% to 90%, depending on the case definition used, The sensitivity of the Duke criteria was also good to excellent, varying from 71% to 99%, again depending on case definition used. Specificity was lower (0-89%). We conclude that use of the Duke criteria will result in little underdiagnosis of IE but that it may result in overdiagnosis of IE; therefore, these criteria should be applied prospectively to determine their clinical usefulness. C1 UNIV PENN, SCH MED, DEPT BIOSTAT & EPIDEMIOL, CTR CLIN EPIDEMIOL & BIOSTAT, PHILADELPHIA, PA 19104 USA. UNIV PENN, SCH MED, DEPT MED, DIV GEN INTERNAL MED, PHILADELPHIA, PA 19104 USA. UNIV PENN, SCH DENT MED, PHILADELPHIA, PA 19104 USA. MED COLL PENN & HAHNEMANN UNIV, DIV INFECT DIS, PHILADELPHIA, PA USA. VET AFFAIRS MED CTR, MED SERV, INFECT DIS SECT, PHILADELPHIA, PA USA. VET AFFAIRS MED CTR, DENT SERV, PHILADELPHIA, PA USA. FU NHLBI NIH HHS [R01 HL 39000] NR 23 TC 35 Z9 35 U1 0 U2 0 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1058-4838 EI 1537-6591 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD JUN PY 1997 VL 24 IS 6 BP 1185 EP 1190 DI 10.1086/513657 PG 6 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA XE366 UT WOS:A1997XE36600026 PM 9195080 ER PT J AU Pons, V Greenspan, D LozadaNur, F McPhail, L Gallant, JE Tunkel, A Johnson, CC McCarty, J Panzer, H Levenstein, M Barranco, A Green, S AF Pons, V Greenspan, D LozadaNur, F McPhail, L Gallant, JE Tunkel, A Johnson, CC McCarty, J Panzer, H Levenstein, M Barranco, A Green, S TI Oropharyngeal candidiasis in patients with AIDS: Randomized comparison of fluconazole versus nystatin oral suspensions SO CLINICAL INFECTIOUS DISEASES LA English DT Article AB A total of 167 human immunodeficiency virus (HIV)-infected patients with oropharyngeal candidiasis were randomly assigned to receive 14 days of therapy with liquid suspension fluconazole (100 mg once daily) or liquid nystatin (500,000 U four times daily). At day 14, 87% of the fluconazole-treated patients were clinically cured, as opposed to 52% in the nystatin-treated group (P <.001), Fluconazole eradicated Candida organisms from the oral flora in 60%, vs, a 6% eradication rate with nystatin (P <.001), The fluconazole group had fewer relapses noted on day 28 (18%, vs, 44% in the nystatin group; P <.001), This relapse difference no longer existed by day 42. Fluconazole oral suspension as a systemic therapy was more effective than liquid nystatin as a topical therapy in the treatment of oral candidiasis in HIV-infected patients and provided a longer disease-free interval before relapse. C1 UNIV CALIF HOSP,DEPT STOMATOL,SAN FRANCISCO,CA 94143. MED COLL PENN & HAHNEMANN UNIV,VET AFFAIRS MED CTR,PHILADELPHIA,PA. CALIF MED RES GRP,FRESNO,CA. PFIZER,US PHARMACEUT GRP,NEW YORK,NY. RP Pons, V (reprint author), UNIV CALIF HOSP,DIV INFECT DIS,521 PARNASSUS AVE,ROOM C-443,SAN FRANCISCO,CA 94143, USA. NR 9 TC 44 Z9 47 U1 0 U2 4 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD JUN PY 1997 VL 24 IS 6 BP 1204 EP 1207 DI 10.1086/513664 PG 4 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA XE366 UT WOS:A1997XE36600029 PM 9195083 ER PT J AU Allan, CJ Argyropoulos, G Bowker, M Zhu, JG Lin, PM Stiver, K Golichowski, A Garvey, WT AF Allan, CJ Argyropoulos, G Bowker, M Zhu, JG Lin, PM Stiver, K Golichowski, A Garvey, WT TI Gestational diabetes mellitus and gene mutations which affect insulin secretion SO DIABETES RESEARCH AND CLINICAL PRACTICE LA English DT Article DE gestational diabetes; gene mutations; impaired insulin secretion; glucokinase; tRNA(leu); mitochondrial DNA ID MITOCHONDRIAL TRNA(LEU(UUR)) GENE; GLUCOKINASE GENE; JAPANESE SUBJECTS; NIDDM; YOUNG; IDENTIFICATION; VARIANTS; MARKER; LOCUS AB We investigated whether genetic mutations known to impair insulin secretion and glucose tolerance are operative in a group of American women with gestational diabetes mellitus. Study groups were comprised of elderly non-diabetic controls (n = 55) with normal glucose tolerance and patients with gestational diabetes (n = 50), together with one family with maturity-onset diabetes of the young (three controls and three affected). No mutations were detected in any exon of the human glucokinase gene or the mitochondrial tRNA(Leu(UUR)) gene by single strand conformational analysis; and direct exon sequencing. Also, X-2 analysis showed no significant association with gestational diabetes for a polymorphism at position -30 (G --> A) of the beta-cell-specific glucokinase gene promoter. We have determined that glucokinase and mitochondrial tRNA(Leu(UUR)) gene mutations, which are known to impair insulin secretion are relatively uncommon and do not constitute a large component of genetic risk for gestational diabetes in the study population. (C) 1997 Elsevier Science Ireland Ltd. C1 MED UNIV S CAROLINA,DEPT MED,DIV ENDOCRINOL,CHARLESTON,SC 29425. MED UNIV S CAROLINA,DEPT MED,DIV ENDOCRINOL DIABET & MED GENET,CHARLESTON,SC 29425. RALPH H JOHNSON VET AFFAIRS MED CTR,CHARLESTON,SC 29425. INDIANA UNIV,SCH MED,DEPT OBSTET & GYNECOL,INDIANAPOLIS,IN 46202. FU NIDDK NIH HHS [DK-47461, DK-38765] NR 24 TC 16 Z9 16 U1 0 U2 3 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0168-8227 J9 DIABETES RES CLIN PR JI Diabetes Res. Clin. Pract. PD JUN PY 1997 VL 36 IS 3 BP 135 EP 141 DI 10.1016/S0168-8227(97)00042-9 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XK925 UT WOS:A1997XK92500001 PM 9237779 ER PT J AU Draznin, B Goalstone, M Leitner, JW AF Draznin, B Goalstone, M Leitner, JW TI Hyperinsulinemia potentiates activation of p21Ras by growth factors. SO DIABETOLOGIA LA English DT Meeting Abstract C1 UNIV COLORADO,HLTH SCI CTR,DENVER,CO. DENVER VAMC,DENVER,CO. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0012-186X J9 DIABETOLOGIA JI Diabetologia PD JUN PY 1997 VL 40 SU 1 BP 151 EP 151 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XG123 UT WOS:A1997XG12300150 ER PT J AU delAguila, MA Pugh, J Reiber, GE AF delAguila, MA Pugh, J Reiber, GE TI How does delay in seeking care for a diabetic foot lesion influence outcome? SO DIABETOLOGIA LA English DT Meeting Abstract C1 VA PUGET SOUND HLTH CARE SYST,SEATTLE,WA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0012-186X J9 DIABETOLOGIA JI Diabetologia PD JUN PY 1997 VL 40 SU 1 BP 1884 EP 1884 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XG123 UT WOS:A1997XG12301873 ER PT J AU Parker, D Kilo, C Baum, J Joynes, J Pistone, B AF Parker, D Kilo, C Baum, J Joynes, J Pistone, B TI Evaluation of an enhanced electrochemical glucose meter system by diabetics and nurses at three clinical sites SO DIABETOLOGIA LA English DT Meeting Abstract C1 W CTY INTERNAL MED,ST LOUIS,MO. ABBOTT NW HOSP,MINNEAPOLIS,MN 55407. DENVER VA MED CTR,DENVER,CO. BAYER CORP,ELKHART,IN. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0012-186X J9 DIABETOLOGIA JI Diabetologia PD JUN PY 1997 VL 40 SU 1 BP 2514 EP 2514 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XG123 UT WOS:A1997XG12302499 ER PT J AU Lembo, T Munakata, J Naliboff, B Fullerton, S Mayer, EA AF Lembo, T Munakata, J Naliboff, B Fullerton, S Mayer, EA TI Sigmoid afferent mechanisms in patients with irritable bowel syndrome SO DIGESTIVE DISEASES AND SCIENCES LA English DT Article DE sigmoid colon; irritable bowel syndrome ID INTESTINAL MOTILITY; RECTAL DISTENSION; COLON; IBS; DISORDER; BALLOON; TONE AB Up to 60% of patients with IBS have lowered perception thresholds in the rectum to balloon distension. The current study sought to test the hypothesis that IBS patients with normal perception thresholds in the rectum show hypersensitivity of afferent pathways in the sigmoid colon. Eleven healthy normal subjects and eight IBS patients with normal rectal perception thresholds underwent a balloon distension protocol in the sigmoid and rectum. Discomfort thresholds, receptive relaxation, compliance, and referral patterns were measured. Although IBS patients had significantly lower discomfort thresholds in the sigmoid when measured as volume, pressure, and wall tension, thresholds were similar to normals. Receptive relaxation and dynamic compliance were significantly decreased in IBS patients in the sigmoid. Referral patterns were similar during sigmoid distention in IBS patients in comparison to normals. Despite normal perception thresholds in rectum and sigmoid, IBS patients show evidence for alterations in rectosigmoid afferent mechanisms. In the sigmoid, this is seen in the form of reduced reflex relaxation and compliance and in the rectum in the form of altered viscerosomatic referral. C1 W LOS ANGELES VET AFFAIRS MED CTR,CURE,DEPT MED,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,DEPT MED,GASTROENTER BIOL CTR,LOS ANGELES,CA 90095. NR 27 TC 48 Z9 48 U1 0 U2 1 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0163-2116 J9 DIGEST DIS SCI JI Dig. Dis. Sci. PD JUN PY 1997 VL 42 IS 6 BP 1112 EP 1120 DI 10.1023/A:1018817132213 PG 9 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA XF479 UT WOS:A1997XF47900002 PM 9201070 ER PT J AU Stoltenberg, RL Madsen, JA Schlack, SC Harms, BA Jacoby, RF AF Stoltenberg, RL Madsen, JA Schlack, SC Harms, BA Jacoby, RF TI Neoplasia in ileal pouch mucosa after total proctocolectomy for juvenile polyposis - Report of a case SO DISEASES OF THE COLON & RECTUM LA English DT Article DE proctocolectomy, restorative; neoplastic syndromes, hereditary; intestinal polyps; colonic polyps; adenomatous polyposis coli; adenomatous polyps ID ILEOANAL ANASTOMOSIS; ULCERATIVE-COLITIS; FAMILIAL POLYPOSIS; TOTAL COLECTOMY; CARCINOMA; PATHOGENESIS; RISK AB PURPOSE: Patients treated with restorative proctocolectomy for familial adenomatous polyposis or ulcerative colitis occasionally develop disease in the ileal pouch similar to that originally present in the colon. We investigated the possibility of analogous involvement in the ileal pouch of juvenile polyposis patients. METHODS: Endoscopic surveillance for neoplasia throughout the gastrointestinal tract was performed, with retrieval of all polypectomy specimens for histologic classification using the criteria of Morson. RESULTS: Multiple large juvenile polyps were found in the ileal pouch of one patient less than 10 years after restorative proctocolectomy for hereditary juvenile polyposis. The pouch was much more severely affected than the proximal ileum, small intestine, or stomach. Although most polyps had a completely benign histologic appearance, three had moderate to severe dysplasia. DISCUSSION: Mucosal changes induced by bacteria or stasis of luminal contents may promote manifestation in the ileal pouch of the disease phenotype usually more evident in the colon. Patients with severe or generalized juvenile polyposis should be considered for periodic endoscopic surveillance of the ileal pouch beginning several years after restorative proctocolectomy. C1 UNIV WISCONSIN,SCH MED,DEPT SURG,MADISON,WI 53792. UNIV WISCONSIN,DEPT PATHOL,MADISON,WI 53792. UNIV WISCONSIN,DEPT MED,GASTROENTEROL SECT,MADISON,WI 53792. UNIV WISCONSIN,CTR COMPREHENS CANC,MADISON,WI 53706. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. FU NCI NIH HHS [U01 CA59352, P30 CA14520] NR 27 TC 2 Z9 2 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0012-3706 J9 DIS COLON RECTUM JI Dis. Colon Rectum PD JUN PY 1997 VL 40 IS 6 BP 726 EP 730 DI 10.1007/BF02140904 PG 5 WC Gastroenterology & Hepatology; Surgery SC Gastroenterology & Hepatology; Surgery GA XE007 UT WOS:A1997XE00700014 PM 9194469 ER PT J AU Elliott, ME Goodfriend, TL Ball, DL Jefcoate, CR AF Elliott, ME Goodfriend, TL Ball, DL Jefcoate, CR TI Angiotensin-responsive adrenal glomerulosa cell proteins: Characterization by protease mapping, species comparison, and specific angiotensin receptor antagonists SO ENDOCRINOLOGY LA English DT Article ID LEYDIG TUMOR-CELLS; ACUTE REGULATORY PROTEIN; STIMULATED ALDOSTERONE SYNTHESIS; MITOCHONDRIAL PROTEINS; CORPUS-LUTEUM; ADRENOCORTICOTROPIC HORMONE; RAPID ACCUMULATION; FASCICULATA CELLS; STEROID-SYNTHESIS; ZONA-GLOMERULOSA AB Angiotensin II (AngII)-stimulated aldosterone synthesis is mediated by the AngII type 1 (AT(1)) receptor and requires ongoing protein synthesis. Hormonally-stimulated turnover of a family of 28- to 30-kDa proteins (p30, or steroidogenic acute regulatory proteins) has been linked to enhanced steroid synthesis in several tissues. Our previous work showed that AngII, dibutyryl cAMP, potassium, and atrial natriuretic peptide affected labeling of a group of eight proteins (four of 28 kDa and four of 30 kDa) in bovine adrenal glomerulosa cells. This report extends our findings in three ways: I) The eight [S-35]-methionine-labeled p30 proteins in bovine cells were compared with each other by chymotryptic peptide mapping. Similarity in maps indicated that the eight proteins share a common primary structure. 2)Dibutyryl cAMP treatment of rat adrenal glomerulosa cells affected the levels of Four 28-kDa proteins and one 35-kDa protein, whereas AngII affected two of the 28-kDa proteins. There were no responsive 80-kDa proteins in rats comparable with those seen in bovine cells. These results indicate a species difference in the affected proteins. 3) The AT(1) receptor antagonist, losartan, inhibited the effects of AngII on aldosterone synthesis and turnover of the p30 proteins in bovine adrenal glomerulosa cells. PD123319, an antagonist specific for the AngII type 2 receptor, did not block AngII-stimulated aldosterone synthesis and had much less effect on p30 protein labeling than did losartan. These results add to the growing body of evidence that this family of p30 or steroidogenic acute regulatory proteins plays a role in the acute regulation of steroidogenesis by a wide variety of stimulatory hormones in several tissues and species. In addition, Iosartan's inhibition of AngII's effects on the p30 proteins is consistent with a key role for these proteins in processes linking occupation of the AT, receptor to stimulation of aldosterone synthesis. C1 UNIV WISCONSIN,SCH MED,DEPT MED,MADISON,WI 53706. UNIV WISCONSIN,SCH MED,DEPT PHARMACOL,MADISON,WI 53706. RP Elliott, ME (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,HYPERTENS RES LAB,ROOM C4114,2500 OVERLOOK TERRACE,MADISON,WI 53705, USA. FU NIDDK NIH HHS [DK-18585] NR 45 TC 9 Z9 9 U1 0 U2 1 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 SN 0013-7227 J9 ENDOCRINOLOGY JI Endocrinology PD JUN PY 1997 VL 138 IS 6 BP 2530 EP 2536 DI 10.1210/en.138.6.2530 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XA025 UT WOS:A1997XA02500044 PM 9165045 ER PT J AU Aspnes, LE Lee, CM Weindruch, R Chung, SS Roecker, EB Aiken, JM AF Aspnes, LE Lee, CM Weindruch, R Chung, SS Roecker, EB Aiken, JM TI Caloric restriction reduces fiber loss and mitochondrial abnormalities in aged rat muscle SO FASEB JOURNAL LA English DT Article DE aging; sarcopenia; mitochondria; skeletal muscle ID CYTOCHROME-C-OXIDASE; ANTIOXIDANT ENZYME-ACTIVITIES; DNA OXIDATIVE DAMAGE; SKELETAL-MUSCLE; DIETARY RESTRICTION; FOOD RESTRICTION; MULTIPLE DELETIONS; RHESUS-MONKEYS; LIFE-SPAN; ATROPHY AB The influence of caloric restriction (CR) initiated at 17 months of age was investigated on selected age-associated measures in skeletal muscle. Tissue from young (3-4 months) ad libitum-fed, old (30-32 months) restricted (35% and 50% CR, designated CR35 and CR50, respectively), and old ad libitum-fed rats (29 months) was studied. CR preserved fiber number and fiber type composition in the vastus lateralis muscle of the CR50 rats. In the old rats from all groups, individual fibers were found with either no detectable cytochrome c oxidase activity (COX-), hyperreactivity for succinate dehydrogenase activity (SDH++; also known as ragged red fibers [RRF]), or both COX- and SDH++. Muscle from the CR50 rats contained significantly fewer COX- and SDH++ fibers than did the muscle from CR35 rats. CR50 rats also had significantly lower numbers of mtDNA deletion products in two (adductor longus and soleus) of the four muscles examined compared to CR35 rats. These data indicate that CR begun in late middle age can retard age-associated fiber loss and fiber type changes, as well as increases in the number of skeletal muscle fibers showing mitochondrial enzyme abnormalities. CR also decreased the accumulation of mtDNA deletions. C1 UNIV WISCONSIN,DEPT NUTR SCI,MADISON,WI 53706. UNIV WISCONSIN,DEPT MED,MADISON,WI 53706. UNIV WISCONSIN,DEPT BIOSTAT,MADISON,WI 53706. WILLIAM S MIDDLETON MEM VET ADM MED CTR,CTR GERIATR RES EDUC & CLIN,MADISON,WI 53706. RP Aspnes, LE (reprint author), UNIV WISCONSIN,DEPT ANIM HLTH & BIOMED SCI,1655 LINDEN DR,MADISON,WI 53706, USA. FU NIA NIH HHS [R01 AG11604, R01 AG10536, P01 AG11915] NR 82 TC 108 Z9 108 U1 0 U2 5 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD JUN PY 1997 VL 11 IS 7 BP 573 EP 581 PG 9 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA XH342 UT WOS:A1997XH34200009 PM 9212081 ER PT J AU Smith, DG Barnes, BC Sands, AK Boyko, EJ Ahroni, JH AF Smith, DG Barnes, BC Sands, AK Boyko, EJ Ahroni, JH TI Prevalence of radiographic foot abnormalities in patients with diabetes SO FOOT & ANKLE INTERNATIONAL LA English DT Article; Proceedings Paper CT 63rd Annual Meeting of the American-Academy-of-Orthopaedic-Surgeons CY FEB 22-24, 1996 CL ATLANTA, GA SP Amer Acad Orthopaed Surgeons ID ULCERATION; PRESSURE; JOINT; MANAGEMENT; NEUROPATHY; CALLUS; FEET AB Clinicians are increasingly aware that mechanical aspects of foot deformities, such as Charcot changes, clawtoes, bunion deformities, or cavus or planus foot deformities, might have an impact on the occurrence, potential healing, and recurrence of foot ulcers. We report the prevalence of plain radiographic changes and attempt to rate the severity of those deformities in the feet of 456 diabetic veteran medicine clinic enrollees. All 456 radiographs were reviewed by orthopaedic surgeons to specifically identify Charcot changes, presence of arterial calcification, dislocation of the lesser toe metatarsophalangeal joints, hallux interphalangeal joint dislocation, and radiographic evidence of previous surgery, Radiographs of 428 patients were taken while weightbearing, and these were reviewed to quantify hallux valgus angles, intermetatarsal 1-2 angles, fifth metatarsal-proximal phalangeal angles, second metatarsal lengths, lateral talocalcaneal and talar-first metatarsal angles, and claw toe deformities. The prevalence of Charcot changes was 1.4% (six subjects), and all had radiographic evidence of midfoot Charcot changes. Other deformities, such as clawtoes, hallux valgus, lesser toe joint dislocations, and alterations in arch freight, are more common in veterans with diabetes. C1 VET AFFAIRS PUGET SOUND HLTH CARE SYST,SURG SERV,SEATTLE,WA. UNIV WASHINGTON,DEPT MED,SEATTLE,WA. VET AFFAIRS PUGET SOUND HLTH CARE SYST,RES & DEV SERV,SEATTLE,WA. RP Smith, DG (reprint author), UNIV WASHINGTON,HARBORVIEW MED CTR,DEPT ORTHOPAED SURG,BOX 359798,325 9TH AVE,SEATTLE,WA 98104, USA. OI Boyko, Edward/0000-0002-3695-192X NR 24 TC 30 Z9 32 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1071-1007 J9 FOOT ANKLE INT JI Foot Ankle Int. PD JUN PY 1997 VL 18 IS 6 BP 342 EP 346 PG 5 WC Orthopedics SC Orthopedics GA XF355 UT WOS:A1997XF35500006 PM 9208292 ER PT J AU Verchere, CB DAlessio, DA Wang, S Andrikopoulos, S Kahn, SE AF Verchere, CB DAlessio, DA Wang, S Andrikopoulos, S Kahn, SE TI Transgenic overproduction of islet amyloid polypeptide (amylin) is not sufficient for islet amyloid formation SO HORMONE AND METABOLIC RESEARCH LA English DT Article; Proceedings Paper CT 2nd Seattle Islet Symposium on Regulation of Islet Growth CY 1996 CL SEATTLE, WA SP ZymoGenet Inc, Seattle, Univ Washington, Diabet Endocrinol Res Ctr DE transgenic mice; non-insulin-dependent diabetes mellitus; insulin; pancreatic beta cell; hyperglycemia; nicotinic acid ID INDUCED INSULIN RESISTANCE; LONG-TERM EXPOSURE; DIABETES-MELLITUS; PANCREATIC-ISLETS; NICOTINIC-ACID; GROWTH-HORMONE; FATTY-ACIDS; BETA-CELLS; IN-VITRO; MICE AB Islet amyloid polypeptide forms islet amyloid deposits in non-insulin-dependent diabetes mellitus, We have generated transgenic mice which express human islet amyloid polypeptide in their pancreatic beta cells yet do not develop islet amyloid deposits despite producing levels of the amyloidogenic human peptide 2-3 fold higher than the native (mouse) peptide. To determine whether marked overproduction of islet amyloid polypeptide is a potential cause of islet amyloid formation, we increased expression of this transgene by producing homozygous transgenic animals and by making heterozygous mice experimentally insulin resistant with nicotinic acid. Pancreatic content of islet amyloid polypeptide-like immunoreactivity in homozygous and nicotinic acid-treated mice was 2-fold (25 +/- 7 fmol/mu g; n = 6) and 3.5-fold (47 +/- 20 fmol/mu g; n = 3) higher, respectively, than that of untreated heterozygous animals (13 +/- 2 fmol/mu g; n = 11; both p < 0.05). Despite this marked increase in production of islet amyloid polypeptide, neither group of mice developed gross islet amyloid deposits even after 16 months of age. We conclude that overproduction of islet amyloid polypeptide, even as produced by extreme insulin resistance, is not in itself sufficient for islet amyloid formation. C1 UNIV WASHINGTON,SEATTLE,WA 98195. RP Verchere, CB (reprint author), VA PUGET SOUND HLTH CARE SYST,DIV METAB ENDOCRINOL & NUTR,1660 S COLUMBIAN WAY,SEATTLE,WA 98108, USA. OI Kahn, Steven/0000-0001-7307-9002 FU NIDDK NIH HHS [DK-12829, DK-17047] NR 33 TC 18 Z9 18 U1 0 U2 0 PU GEORG THIEME VERLAG PI STUTTGART PA P O BOX 30 11 20, D-70451 STUTTGART, GERMANY SN 0018-5043 J9 HORM METAB RES JI Horm. Metab. Res. PD JUN PY 1997 VL 29 IS 6 BP 311 EP 316 DI 10.1055/s-2007-979042 PG 6 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XH664 UT WOS:A1997XH66400011 PM 9230354 ER PT J AU Duerinckx, AJ AF Duerinckx, AJ TI MRI of coronary arteries SO INTERNATIONAL JOURNAL OF CARDIAC IMAGING LA English DT Article DE heart; MRI; coronary angiography; MR angiography (MRA); coronary MRA; coronary flow; coronary vessel ID MAGNETIC-RESONANCE ANGIOGRAPHY; CONTRAST AB Magnetic Resonance Angiography (MRA) of the coronary arteries has recently become possible due to the development of a new group of ultrafast imaging sequences. Although the role of coronary MR angiography in screening for coronary artery lesions has not yet been established, coronary MR angiography has been very successful in the detection of coronary artery variants, and the imaging of coronary stents and bypass grafts. Variants of these new MRI techniques can also quantitate velocity in native coronary arteries. Coronary MR angiographic techniques can be subdivided in breath-hold (single or repeated breath-hold) and non-breath-hold techniques. Most of the clinical experience so far has been with a single breath-hold technique, and was limited to cooperative patients. The recent introduction of navigator pulses for real-time respiratory gating or triggering allows non-breath-herd or repeated breath-hold 3-D coronary MR angiography, and will allow a more widespread use of this technique. Notwithstanding the progress being made and the excitement created by the prospect of a noninvasive coronary artery screening tool, several key technical problems remain unresolved and are now being addressed by the scientific and clinical community. This paper reviews ongoing research in coronary MR angiography. C1 UNIV CALIF LOS ANGELES,MED CTR,DEPT RADIOL,LOS ANGELES,CA 90095. RP Duerinckx, AJ (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,SERV RADIOL,11301 WILSHIRE BLVD,MRI BLD 507,LOS ANGELES,CA 90073, USA. NR 52 TC 8 Z9 8 U1 0 U2 1 PU KLUWER ACADEMIC PUBL PI DORDRECHT PA SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS SN 0167-9899 J9 INT J CARDIAC IMAG JI Int. J. Card. Imaging PD JUN PY 1997 VL 13 IS 3 BP 191 EP 197 DI 10.1023/A:1005791317440 PG 7 WC Cardiac & Cardiovascular Systems; Radiology, Nuclear Medicine & Medical Imaging SC Cardiovascular System & Cardiology; Radiology, Nuclear Medicine & Medical Imaging GA XH809 UT WOS:A1997XH80900003 PM 9220281 ER PT J AU Boyko, EJ Ahroni, JH Davignon, D Stensel, V Prigeon, RL Smith, DG AF Boyko, EJ Ahroni, JH Davignon, D Stensel, V Prigeon, RL Smith, DG TI Diagnostic utility of the history and physical examination for peripheral vascular disease among patients with diabetes mellitus SO JOURNAL OF CLINICAL EPIDEMIOLOGY LA English DT Article ID ARTERIAL-DISEASE; LIKELIHOOD RATIOS; BLOOD-PRESSURE; FOOT; AMPUTATION; MORTALITY; LEVEL AB Background: We assessed the value of the medical history and physical examination in the diagnosis of peripheral vascular disease in diabetic subjects. Methods: We performed a cross-sectional study in 631 diabetic veteran enrollees of a general internal medicine clinic that compared data obtained from a history and clinical evaluation with the presence of severe peripheral vascular disease defined as an ankle-arm index (AAI) less than or equal to 0.5 derived from Doppler blood pressure measurement. Results: We identified 90 limbs with an AAI less than or equal to 0.5. Results presented below apply to the right leg, but do not differ from the left. Diminished or absent foot peripheral pulses (sensitivity 65%, specificity 78%), venous filling time > 20 sec (sensitivity 22%, specificity 93.9%), age > 65 years (sensitivity 83%, specificity 54%), claudication symptoms in < 1 block (sensitivity 50%, specificity 87%), and patient reported history of physician diagnosed peripheral vascular disease (PVD) (sensitivity 80%, specificity 70%) had the largest positive (or smallest negative) likelihood ratios. Capillary refill time > 5 sec or foot characteristics (absent hair, blue/purple color, skin coolness, or atrophy) conveyed little diagnostic information. Individual factors did not change disease probability to a clinically important degree. A stepwise logistic regression model identified four factors significantly (P < 0.05) associated with low AAI: absent or diminished peripheral pulses, patient reported history of PVD, age, and venous filling time. Substitution of < 1 block claudication for PVD history in this model resulted in a small reduction in model accuracy. Conclusions: Many purportedly useful historical and exam findings need not be elicited in diabetic patients suspected of having severe peripheral vascular disease, since most information related to probability of this disorder may be obtained from patient age, self-reported history of physician diagnosed PVD (or < 1 block claudication), peripheral pulse palpation, and venous filling time. (C) 1997 Elsevier Science Inc. C1 UNIV WASHINGTON,DEPT MED,SEATTLE,WA 98108. VA PUGET SOUND HLTH CARE SYST,DEPT ORTHOPED,SEATTLE,WA 98108. UNIV WASHINGTON,DEPT ORTHOPED,SEATTLE,WA 98108. RP Boyko, EJ (reprint author), VA PUGET SOUND HLTH CARE SYST,MED SERV,1660 S COLUMBIAN WAY,SEATTLE,WA 98108, USA. OI Boyko, Edward/0000-0002-3695-192X NR 32 TC 43 Z9 43 U1 1 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0895-4356 J9 J CLIN EPIDEMIOL JI J. Clin. Epidemiol. PD JUN PY 1997 VL 50 IS 6 BP 659 EP 668 DI 10.1016/S0895-4356(97)00005-X PG 10 WC Health Care Sciences & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA XH671 UT WOS:A1997XH67100006 PM 9250264 ER PT J AU Nakabayashi, T Letterio, JJ Geiser, AG Kong, LP Ogawa, N Zhao, WG Koike, T Fernandes, G Dang, H Talal, N AF Nakabayashi, T Letterio, JJ Geiser, AG Kong, LP Ogawa, N Zhao, WG Koike, T Fernandes, G Dang, H Talal, N TI Up-regulation of cytokine mRNA, adhesion molecule proteins, and MHC class II proteins in salivary glands of TGF-beta 1 knockout mice - MHC class II is a factor in the pathogenesis of TGF-beta 1 knockout mice SO JOURNAL OF IMMUNOLOGY LA English DT Article ID GROWTH-FACTOR-BETA; SJOGRENS-SYNDROME; HUMAN-LYMPHOCYTES; INTERFERON-GAMMA; DEFICIENT MICE; TGF-BETA; EXPRESSION; MOUSE; ICAM-1; DEATH AB Mice homozygous for a disrupted TGF-beta 1 allele develop multiple lymphoproliferative disorders similar to those seen in the pseudolymphoma of Sjogren's syndrome. At 2 wk of age, these TGF-beta 1 mutant mice begin to develop wasting syndrome and die at around 4 to 5 wk of age, We studied salivary glands from symptomatic mutant mice >14 days of age, Reverse transcriptase-PCR analysis showed up-regulation of proinflammatory cytokine genes such as IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-6, IL-10, and IFN-gamma in these mutant mice. Enhanced expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule (VCAM-1), and MHC class II as well as CD4-positive T lymphocyte infiltration was detected by immunostaining, To elucidate the role of MHC class II, salivary glands from TGF-beta 1/MHC class II double knockout mice were used to investigate the expression of adhesion molecules and MHC class II, In spite of the existence of basal intercellular adhesion molecule-1 expression on vessels, there was neither MHC class II expression, enhanced vascular cell adhesion molecule-1 expression, nor lymphocytic infiltration in the salivary glands, These results suggest that MHC class II plays a significant role in the pathogenesis of TGF-beta 1 mutant mice, Although the mechanism that initiates multiple inflammatory diseases in these mice remains unclear, the context reported here would provide insight into the immunopathology of Sjogren's syndrome. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV CLIN IMMUNOL,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NCI,CHEMOPREVENT LAB,NIH,BETHESDA,MD 20892. LILLY CORP CTR,LILLY RES LABS,INDIANAPOLIS,IN 46285. HOKKAIDO UNIV,SCH MED,SAPPORO,HOKKAIDO 060,JAPAN. FU NIDCR NIH HHS [DE 10863, DE 09311] NR 41 TC 34 Z9 34 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD JUN 1 PY 1997 VL 158 IS 11 BP 5527 EP 5535 PG 9 WC Immunology SC Immunology GA XA063 UT WOS:A1997XA06300061 PM 9164977 ER PT J AU Aquino, DA Capello, E Weisstein, J Sanders, V Lopez, C Tourtellotte, WW Brosnan, CF Raine, CS Norton, WT AF Aquino, DA Capello, E Weisstein, J Sanders, V Lopez, C Tourtellotte, WW Brosnan, CF Raine, CS Norton, WT TI Multiple sclerosis: Altered expression of 70- and 27-kDa heat shock proteins in lesions and myelin SO JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY LA English DT Article ID EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; BASIC-PROTEIN; T-CELLS; MONOCLONAL-ANTIBODIES; CANDIDATE AUTOANTIGEN; ACTIN POLYMERIZATION; CEREBROSPINAL-FLUID; GENE REARRANGEMENTS; PROTEOLIPID PROTEIN; INCREASED FREQUENCY AB Recent studies have implicated heat shock proteins (HSP) in the pathogenesis of the multiple sclerosis (MS) lesion. Expression of the 73 kDa constitutive HSP (HSC70), the 72 kDa stress-inducible HSP (HSP70), and the 27 kDa small HSP (HSP27) was analyzed in white matter and myelin from central nervous system (CNS) tissue of MS and normal subjects using a combination of immunocytochemistry and quantitative immunoblotting. Plaques of all types were sharply defined by reduced immunostaining for HSC70, and shown by immunoblotting to contain 30 to 50% less HSC70 than surrounding white matter or normal tissue. Ln contrast, HSP27 was markedly enhanced 2.5- to 4-fold in plaque regions, especially in fibrous astrocytes and in hyperplastic interfascicular oligodendrocytes at the lesion edge. HSP70 was less abundant than HSC70, and no significant differences in HSP70 levels were noted between MS and normal white matter. Myelin isolated from active plaques contained 3- to 4-fold more HSC70 than normal myelin. Pronounced expression of HSP70 and HSP27 was also found in MS myelin, although neither protein was detected in normal myelin. Thus, white matter undergoing immune-mediated destruction in MS was associated with altered distribution and expression of HSC70 and HSP27. These changes may initially serve to protect myelin from further destruction and facilitate repair; however, enhanced expression of HSC70, HSP70, and HSP27 in myelin may subsequently present as additional immune targets involved in the progression of disease. C1 YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT PATHOL,BRONX,NY 10461. YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT NEUROSCI,BRONX,NY 10461. UNIV CALIF LOS ANGELES,BRAIN RES INST,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,NEUROL SERV,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,RES SERV,LOS ANGELES,CA 90073. RP Aquino, DA (reprint author), YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT NEUROL,1300 MORRIS PK AVE,F-140,BRONX,NY 10461, USA. FU NINDS NIH HHS [NS-23705, NS-30319]; PHS HHS [MS-08952] NR 46 TC 62 Z9 64 U1 0 U2 3 PU AMER ASSN NEUROPATHOLOGISTS INC PI LAWRENCE PA 1041 NEW HAMPSHIRE ST, LAWRENCE, KS 66044 SN 0022-3069 J9 J NEUROPATH EXP NEUR JI J. Neuropathol. Exp. Neurol. PD JUN PY 1997 VL 56 IS 6 BP 664 EP 672 PG 9 WC Clinical Neurology; Neurosciences; Pathology SC Neurosciences & Neurology; Pathology GA XD281 UT WOS:A1997XD28100004 PM 9184657 ER PT J AU Trimble, MR Mendez, MF Cummings, JL AF Trimble, MR Mendez, MF Cummings, JL TI Neuropsychiatric symptoms from the temporolimbic lobes SO JOURNAL OF NEUROPSYCHIATRY AND CLINICAL NEUROSCIENCES LA English DT Article ID INTERICTAL BEHAVIOR; EMISSION TOMOGRAPHY; TEMPORAL LOBES; MOOD DISORDERS; EPILEPSY; SCHIZOPHRENIA; HYPERGRAPHIA; DEPRESSION; PSYCHOSIS; ANXIETY AB Neuropsychiatric symptoms are common manifestations of temporolimbic. lesions. These neuropsychiatric symptoms result from disturbances of specific temporolimbic networks, including medial limbic circuits, lateral limbic circuits, and the ''extended'' amygdala. Moreover, temporolimbic networks interface with multiple cortical and subcortical circuits that modulate emotional behavior and affect. This article not only reviews these behaviorally relevant aspects of temporolimbic neuroanatomy, but also describes positive, productive symptoms from the temporal lobes. The Kluver-Bucy syndrome, the Gastaut-Geschwind syndrome, emotional or mood disorders, delusions, anxiety and associative disorders, and neurovegetative symptoms are discussed, as well as amnesia, the signature temporolimbic disturbance of cognition. The temporolimbic lobe is a classic example of a widely distributed circuit within the brain that has behaviorally relevant manifestations. C1 UNIV CALIF LOS ANGELES,SCH MED,REED NEUROL RES CTR,DEPT NEUROL,LOS ANGELES,CA 90095. UNIV CALIF LOS ANGELES,SCH MED,DEPT PSYCHIAT,LOS ANGELES,CA 90095. INST NEUROL,LONDON WC1N 3BG,ENGLAND. W LOS ANGELES VET AFFAIRS MED CTR,PSYCHIAT SERV,NEUROBEHAV & NEUROPSYCHIAT PROGRAM,LOS ANGELES,CA 90073. FU NIA NIH HHS [AG10123] NR 86 TC 42 Z9 42 U1 0 U2 1 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 0895-0172 J9 J NEUROPSYCH CLIN N JI J. Neuropsychiatr. Clin. Neurosci. PD SUM PY 1997 VL 9 IS 3 BP 429 EP 438 PG 10 WC Clinical Neurology; Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA XR782 UT WOS:A1997XR78200009 PM 9276844 ER PT J AU Frueh, BC Gold, PB deArellano, MA Brady, KL AF Frueh, BC Gold, PB deArellano, MA Brady, KL TI A racial comparison of combat veterans evaluated for PTSD SO JOURNAL OF PERSONALITY ASSESSMENT LA English DT Article ID POSTTRAUMATIC-STRESS-DISORDER; VIETNAM VETERANS; SCALE; BLACK; RELIABILITY; ETHNICITY; VALIDITY; IMPACT; WHITE AB This study attempted to replicate the work of Frueh, Smith, and Libet (1996), which showed racial differences on psychological measures of dissociation/thought disturbance and the Minnesota Multiphasic Personality Inventory-2 (MMPI-2) F-K index in combat veterans evaluated for posttraumatic stress disorder (PTSD). Veterans completed the Beck Depression Inventory, Mississippi Scale for Combat-Related PTSD, a fixed-response format version of the Dissociative Experiences Scale (DES-FRF), and MMPI-2. prior to treatment at a Veterans affairs hospital outpatient PTSD clinic, Contrary to expectation, significant racial differences on the DES-FRF, MMPI-2 validity scales, and MMPI-2 Scales 6 and 8 were not found. Consistent with the previous study, no racial differences on measures of anxiety, depression, or PTSD symptomatology were found; nor were there racial differences on clinician ratings of global assessment of functioning or on most categories of psychiatric diagnoses. This suggests that Black and White combat veterans evaluated for PTSD do not differ with regard to reported manifestation or severity of psychopathology. C1 MED UNIV S CAROLINA,DEPT PSYCHIAT & BEHAV SCI,NATL CRIME VICTIM RES & TREATMENT CTR,CHARLESTON,SC 29425. RP Frueh, BC (reprint author), RALPH H JOHNSON VA MED CTR,MENTAL HLTH SERV 116,109 BEE ST,CHARLESTON,SC 29401, USA. NR 32 TC 18 Z9 18 U1 1 U2 3 PU LAWRENCE ERLBAUM ASSOC INC PI MAHWAH PA 10 INDUSTRIAL AVE, MAHWAH, NJ 07430-2262 SN 0022-3891 J9 J PERS ASSESS JI J. Pers. Assess. PD JUN PY 1997 VL 68 IS 3 BP 692 EP 702 DI 10.1207/s15327752jpa6803_14 PG 11 WC Psychology, Clinical; Psychology, Social SC Psychology GA WZ566 UT WOS:A1997WZ56600014 PM 9170304 ER PT J AU Strack, S Lorr, M AF Strack, S Lorr, M TI Invited essay: The challenge of differentiating normal and disordered personality SO JOURNAL OF PERSONALITY DISORDERS LA English DT Article ID TAXONOMY; NUMBER AB By separating personality disorders from other psychiatric conditions and requiring mental health professionals to assess the personalities of all their patients, DSM-III Axis II created an explosion of ideas and research on the nature and structure of personality, Since 1980, theorists and researchers from previously segregated camps have come together to address a number of important taxonomic issues, including the relationship between normal and disordered character, In this article, we place the challenge of differentiating normal and abnormal personality in historical perspective. and outline major theories, models, and methods that inform personologists in their quest. The complexity of personality, and the differences in the way people view the subject matter, ensure that there will be several research lines in the next generation, Progress in the field can be quickened by refinements in theory, the development of more assessment instruments that tap both normal and abnormal traits, and empirical studies that follow well-match groups of normals and patients over significant time periods. C1 CATHOLIC UNIV AMER,INST LIFE CYCLE,WASHINGTON,DC 20064. RP Strack, S (reprint author), US DEPT VET AFFAIRS,OUTPATIENT CLIN,PSYCHOL SERV 116B,351 E TEMPLE ST,LOS ANGELES,CA 90012, USA. NR 88 TC 12 Z9 12 U1 0 U2 1 PU GUILFORD PUBLICATIONS INC PI NEW YORK PA 72 SPRING STREET, NEW YORK, NY 10012 SN 0885-579X J9 J PERS DISORD JI J. Pers. Disord. PD SUM PY 1997 VL 11 IS 2 BP 105 EP 122 PG 18 WC Psychiatry SC Psychiatry GA XF773 UT WOS:A1997XF77300001 PM 9203106 ER PT J AU Bernstein, DP Kasapis, C Bergman, A Weld, E Mitropoulou, V Horvath, T Klar, HM Silverman, J Siever, LJ AF Bernstein, DP Kasapis, C Bergman, A Weld, E Mitropoulou, V Horvath, T Klar, HM Silverman, J Siever, LJ TI Assessing axis II disorders by informant interview SO JOURNAL OF PERSONALITY DISORDERS LA English DT Article ID DIAGNOSING PERSONALITY-DISORDERS; DSM-III AB Although much of personality disorder research depends on diagnostic data obtained directly from patients, this approach has rarely been compared to interviews with knowledgeable informants. The purpose of this study was to determine the diagnostic agreement between these two assessment methods, as well as their relative contribution to the formulation of consensus diagnoses, Sixty-two psychiatric patients were assessed directly with the Structured Interview for DSM-III Personality Disorders (SIDP), and were asked to nominate an informant - either a family member or friend - to provide information about the patient in an interview with the same instrument, Informant interviews were conducted blind to patient-based information whenever feasible, and diagnostic consensus was achieved by an independent review of all available data by a senior clinician, Diagnostic agreement between patient-based and informant-based personality disorder interview was poor, confirming the findings of two previous studies, Information obtained from patients tended to be given greater weight in formulating consensus diagnoses than information provided by informants. However, about one quarter of diagnostic disagreements were resolved in favor of informant-based information. in contrast to a previous study, the inclusion of informant information did not appear to reveal greater psychopathology in patients. We conclude that supplementing direct patient interview with data provided by a knowledgeable informant appears to enhance the resolution of some personality disorder diagnoses. The utility of informant Interviews may depend on an analysis of the costs and benefits of this additional degree of descriptive refinement. C1 COLUMBIA UNIV,NEW YORK,NY. NEW YORK STATE PSYCHIAT INST & HOSP,NEW YORK,NY 10032. NORTHPORT VET AFFAIRS MED CTR,NORTHPORT,NY. RP Bernstein, DP (reprint author), BRONX VET ADM MED CTR,PSYCHIAT SERV 116A,130 W KINGSBRIDGE RD,BRONX,NY 10468, USA. FU NIMH NIH HHS [MH42827-07] NR 18 TC 25 Z9 25 U1 0 U2 1 PU GUILFORD PUBLICATIONS INC PI NEW YORK PA 72 SPRING STREET, NEW YORK, NY 10012 SN 0885-579X J9 J PERS DISORD JI J. Pers. Disord. PD SUM PY 1997 VL 11 IS 2 BP 158 EP 167 PG 10 WC Psychiatry SC Psychiatry GA XF773 UT WOS:A1997XF77300005 PM 9203110 ER PT J AU Petrecca, K Amellal, F Laird, DW Cohen, SA Shrier, A AF Petrecca, K Amellal, F Laird, DW Cohen, SA Shrier, A TI Sodium channel distribution within the rabbit atrioventricular node as analysed by confocal microscopy SO JOURNAL OF PHYSIOLOGY-LONDON LA English DT Article ID JUNCTION PROTEIN PHENOTYPES; CONDUCTION SYSTEM; RAT-HEART; PURKINJE-FIBERS; GAP-JUNCTIONS; PATTERNS; MUSCLE; CONNEXIN43; TISSUES AB 1. Paired 20 mu m thick sections of fresh frozen tissue taken from the frontal plane of the rabbit atrioventricular (AV) nodal region were processed for histology and immunohistochemistry. Confocal microscopy was used to image the distribution of sodium channels using IgG (R12) developed against a highly conserved sequence in the interdomain 3-4 region of cloned sodium channels. 2. In ventricular and atrial cells, sodium channel immunofluorescence was localized to lateral membranes and T-tubules. In the open AV node, levels of sodium channel immunofluorescence in the transitional cell zone and in the lower nodal cell tract were comparable to that found in the atrial and ventricular myocardium. 3. In the enclosed AV node a gradation of sodium channel immunofluorescence is present such that peripherally located circumferential transitional cells display high levels of immunofluorescence, comparable to that of atrial and ventricular myocardium, while centrally located midnodal cells display decreased levels of or no immunofluorescence. 4. In order to correlate the distribution of sodium channels with the distribution of gap junctions, we used IgG directed against the carboxyl terminus of connexin43 (CT-360). Ventricular cell immunofluorescence was localized primarily to the intercalated disk region, while in the AV node, the pattern of distribution was found to be similar to that of sodium channels. 5. The reduced levels of and/or absence of immunofluorescence in the midnodal cell region indicates a paucity of sodium channel and connexin43 protein expression in this region of the AV node that would favour slow impulse conduction. C1 MCGILL UNIV,DEPT PHYSIOL,MONTREAL,PQ,CANADA. MCGILL UNIV,DEPT ANAT & CELL BIOL,MONTREAL,PQ,CANADA. UNIV PENN,SCH MED,DEPT MED,PHILADELPHIA,PA 19104. VET AFFAIRS MED CTR,PHILADELPHIA,PA. RI Laird, Dale /E-4176-2015 NR 33 TC 35 Z9 35 U1 0 U2 5 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 40 WEST 20TH STREET, NEW YORK, NY 10011-4211 SN 0022-3751 J9 J PHYSIOL-LONDON JI J. Physiol.-London PD JUN 1 PY 1997 VL 501 IS 2 BP 263 EP 274 DI 10.1111/j.1469-7793.1997.263bn.x PG 12 WC Neurosciences; Physiology SC Neurosciences & Neurology; Physiology GA XF545 UT WOS:A1997XF54500002 PM 9192299 ER PT J AU Richey, GK Gorp, WG AF Richey, GK Gorp, WG TI The impact of psychosocial variables on immune system functioning in a sample of HIV-positive males SO JOURNAL OF PSYCHOPATHOLOGY AND BEHAVIORAL ASSESSMENT LA English DT Meeting Abstract C1 PLYMOUTH STATE COLL,PLYMOUTH,DEVON,ENGLAND. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. NR 0 TC 0 Z9 0 U1 0 U2 0 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0882-2689 J9 J PSYCHOPATHOL BEHAV JI J. Psychopathol. Behav. Assess. PD JUN PY 1997 VL 19 IS 2 BP 179 EP 180 PG 2 WC Psychology, Clinical SC Psychology GA YL136 UT WOS:A1997YL13600015 ER PT J AU Moore, CA Fishman, IJ Hirshkowitz, M AF Moore, CA Fishman, IJ Hirshkowitz, M TI Evaluation of erectile dysfunction and sleep-related erections SO JOURNAL OF PSYCHOSOMATIC RESEARCH LA English DT Review DE sleep; impotence; erections; erectile dysfunction; nocturnal penile tumescence ID NOCTURNAL PENILE TUMESCENCE; SMOOTH-MUSCLE CELLS; CORPUS CAVERNOSUM; NITRIC-OXIDE; MEN; TESTOSTERONE; RELAXATION; PREVALENCE; IMPOTENCE; DOGS AB Significant advances in this past decade have improved our understanding of erectile physiology. A variety of tests are available for diagnosing impotence, SRE testing provides objective physiological information that is useful for indexing erectile capability and formulating a rational treatment plan. As such, SRE testing is a powerful noninvasive tool for assessing dysfunction. Nonetheless, in making a final diagnosis, the skillful clinician relies on more than one assessment parameter and on clinical acumen. (C) 1997 Elsevier Science Inc. C1 BAYLOR COLL MED,HOUSTON,TX 77030. RP Moore, CA (reprint author), DEPT VET AFFAIRS MED CTR,SLEEP CTR 116A,2002 HOLCOMBE BLVD,HOUSTON,TX 77030, USA. NR 33 TC 7 Z9 9 U1 1 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0022-3999 J9 J PSYCHOSOM RES JI J. Psychosomat. Res. PD JUN PY 1997 VL 42 IS 6 BP 531 EP 539 DI 10.1016/S0022-3999(97)00014-7 PG 9 WC Psychiatry SC Psychiatry GA XE719 UT WOS:A1997XE71900004 PM 9226600 ER PT J AU Hirshkowitz, M Moore, CA OConnor, S Bellamy, M Cunningham, GR AF Hirshkowitz, M Moore, CA OConnor, S Bellamy, M Cunningham, GR TI Androgen and sleep-related erections SO JOURNAL OF PSYCHOSOMATIC RESEARCH LA English DT Article DE testosterone; sleep; erections ID NOCTURNAL PENILE TUMESCENCE; HYPOGONADAL MEN; PLASMA TESTOSTERONE; SERUM TESTOSTERONE; MEDROXYPROGESTERONE ACETATE; CIRCADIAN-RHYTHM; REPLACEMENT; BEHAVIOR; GONADOTROPIN; PROLACTIN AB Sleep-related penile erections provide a unique opportunity to objectively study erectile physiology in man. Testosterone is one of several factors involved in normal sexual function and testosterone reduction can be achieved by administering luteinizing-hormone releasing-hormone agonists (LHRH-A). In this study, ten healthy, young adult males were administered LHRH-A or placebo for a 12-week period. Subjects taking LHRH-A had a marginally significant decline in sleep-related erection duration at week 4 and significant reductions at weeks 8 and 12. By contrast, no statistically reliable change was found for the number of erections over the course of study. Maximum circumference increase during sleep erections showed mixed results. These results indicate that, whereas androgen reduction adversely affects sleep-related erections, it does not eliminate them over a 12-week trial in healthy young adult men. Further study in a larger sample is needed. Nonetheless, these preliminary findings support androgen having an important role in sleep-related erections. (C) 1997 Elsevier Science Inc. C1 BAYLOR COLL MED,HOUSTON,TX 77030. RP Hirshkowitz, M (reprint author), DEPT VET AFFAIRS MED CTR,SLEEP CTR 116A,2002 HOLCOMBE BLVD,HOUSTON,TX 77030, USA. NR 20 TC 27 Z9 29 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0022-3999 J9 J PSYCHOSOM RES JI J. Psychosomat. Res. PD JUN PY 1997 VL 42 IS 6 BP 541 EP 546 DI 10.1016/S0022-3999(97)00006-8 PG 6 WC Psychiatry SC Psychiatry GA XE719 UT WOS:A1997XE71900005 PM 9226601 ER PT J AU Ellabban, A Schumacher, HR AF Ellabban, A Schumacher, HR TI Erythema elevatum diutinum with extensive acro-osteolysis SO JOURNAL OF RHEUMATOLOGY LA English DT Article DE erythema elevatum diutinum; acro-osteolysis; leukocytoclastic vasculitis ID PATIENT AB Erythema elevatum diutinum (EED) is a rare chronic skin disease with cutaneous vasculitis, characterized by the absence of systemic vasculopathy, We describe a 79-year-old white woman who has been followed with the diagnosis of EED for 27 years, Our patient has a previously unreported combination of EED and acro-osteolysis. After reviewing possible reasons for the association, her vasculitis seems the most likely factor. C1 DEPT VET AFFAIRS MED CTR,ARTHRIT IMMUNOL CTR,PHILADELPHIA,PA 19104. UNIV PENN,SCH MED,DIV RHEUMATOL,PHILADELPHIA,PA 19104. NR 9 TC 5 Z9 5 U1 0 U2 0 PU J RHEUMATOL PUBL CO PI TORONTO PA 920 YONGE ST, SUITE 115, TORONTO ON M4W 3C7, CANADA SN 0315-162X J9 J RHEUMATOL JI J. Rheumatol. PD JUN PY 1997 VL 24 IS 6 BP 1203 EP 1205 PG 3 WC Rheumatology SC Rheumatology GA XD791 UT WOS:A1997XD79100033 PM 9195535 ER PT J AU Silverman, SL Cranney, A AF Silverman, SL Cranney, A TI Quality of life measurement in osteoporosis SO JOURNAL OF RHEUMATOLOGY LA English DT Article; Proceedings Paper CT Conference on the Outcome Measures in Arthritis Clinical Trials (OMERACT III) CY APR 16-19, 1996 CL CAIRNS, AUSTRALIA DE osteoporosis; quality of life ID VERTEBRAL COMPRESSION FRACTURES; HEALTH-STATUS; OF-LIFE; FUNCTIONAL STATUS; QUESTIONNAIRE; INSTRUMENT; COMPLAINTS; ARTHRITIS; WOMEN AB Quality of life measurement may be helpful in randomized clinical trials in osteoporosis to assess therapeutic tradeoffs and compare effects of different interventions. Quality of life measures include generic measures, disease targeted measures, and performance based measures. Disease targeted measures increase the coverage of domains that are of particular importance to the patient with established osteoporosis. Several disease targeted measures are currently available. These measures show discriminant validity, but data on longitudinal responsiveness and validity in randomized clinical trials are not yet available. C1 OTTAWA GEN HOSP,OTTAWA,ON K1H 8L6,CANADA. RP Silverman, SL (reprint author), UNIV CALIF LOS ANGELES,DIV RHEUMATOL 111J,W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073, USA. NR 29 TC 27 Z9 28 U1 2 U2 3 PU J RHEUMATOL PUBL CO PI TORONTO PA 920 YONGE ST, SUITE 115, TORONTO ON M4W 3C7, CANADA SN 0315-162X J9 J RHEUMATOL JI J. Rheumatol. PD JUN PY 1997 VL 24 IS 6 BP 1218 EP 1221 PG 4 WC Rheumatology SC Rheumatology GA XD791 UT WOS:A1997XD79100038 PM 9195540 ER PT J AU Shekelle, PG Coulter, I AF Shekelle, PG Coulter, I TI Cervical spine manipulation: Summary report of a systematic review of the literature and a multidisciplinary expert panel SO JOURNAL OF SPINAL DISORDERS LA English DT Review ID RANDOMIZED CLINICAL-TRIAL; LOW-BACK-PAIN; NECK COMPLAINTS; MANUAL THERAPY; PHYSIOTHERAPY C1 W LOS ANGELES VET AFFAIRS MED CTR,SANTA MONICA,CA. NR 21 TC 37 Z9 37 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0895-0385 J9 J SPINAL DISORD JI J. Spinal Disord. PD JUN PY 1997 VL 10 IS 3 BP 223 EP 228 PG 6 WC Clinical Neurology; Orthopedics SC Neurosciences & Neurology; Orthopedics GA XG955 UT WOS:A1997XG95500007 PM 9213278 ER PT J AU Morrison, DA Sacks, J Barbiere, CC Sethi, G AF Morrison, DA Sacks, J Barbiere, CC Sethi, G TI PTCA comes to the bifurcations in the road: What the VA has to offer SO JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY LA English DT Editorial Material ID TRANSLUMINAL CORONARY ANGIOPLASTY; ASSOCIATION TASK-FORCE; UNSTABLE ANGINA; BYPASS-SURGERY; FOLLOW-UP; HIGH-RISK; CARDIOLOGY; REVASCULARIZATION; GUIDELINES; SURVIVAL C1 UNIV COLORADO,HLTH SCI CTR,DENVER,CO. US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,COOPERAT STUDIES PROGRAM COORDINATING CTR,HINES,IL 60141. VET AFFAIRS MED CTR,TUCSON,AZ. RP Morrison, DA (reprint author), DENVER VET AFFAIRS MED CTR,CARDIOL SECT,1055 CLERMONT ST 111-B,DENVER,CO 80220, USA. NR 24 TC 3 Z9 3 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0735-1097 J9 J AM COLL CARDIOL JI J. Am. Coll. Cardiol. PD JUN PY 1997 VL 29 IS 7 BP 1512 EP 1514 PG 3 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA XB643 UT WOS:A1997XB64300016 PM 9180112 ER PT J AU Flynn, MA Herbert, V Nolph, GB Krause, G AF Flynn, MA Herbert, V Nolph, GB Krause, G TI Atherogenesis and the homocysteine-folate-cobalamin triad: Do we need standardized analyses? SO JOURNAL OF THE AMERICAN COLLEGE OF NUTRITION LA English DT Article DE homocysteine; cobalamin; holotranscobalamin; folate; methionine ID CORONARY-ARTERY DISEASE; HOLO-TRANSCOBALAMIN-II; HOLOTRANSCOBALAMIN-II; PLASMA HOMOCYST(E)INE; ELDERLY POPULATION; ATROPHIC GASTRITIS; HEALTHY-SUBJECTS; SERUM; DEFICIENCY; VITAMIN-B-12 AB Background: Bioscientists, physicians and nutritionists are newly interested in the homocysteine-folate cobalamin triad, in part because homocysteine may be important both in atherogenesis and thrombogenesis. Homocysteine imbalance may be an early marker for cobalamin disorders because cobalamin is a cofactor in remethylation of homocysteine to methionine. Methods: In 139 men and 32 women of similar mean age of 65 years, we measured markers which have been cited as risk for atherosclerosis: serum homocysteine, folate, total cobalamin, holotranscobalamin I and II, (TCI and TCII), total serum cholesterol (SCHOL), high density lipoprotein cholesterol (HDLC), triglycerides (STG) as well as red blood cell (RBC) folate, food records and body composition by whole body counting of potassium-forty (K-40). Results: Statistical relationships among the data showed healthy women had lower mean serum homocysteine and their mean RBC folate and TCI and TCII were higher than men. Eighty-three subjects had TCII much lower than 60 pg/ml (subnormal), yet only 11 of these men and two women had total cobalamin <200 pg/ml (abnormal). Fifty-two subjects with serum homocysteine greater than 17.5 nmol/ml had TCII less than 60 pg/ml, suggesting serum homocysteine may be a marker for early cobalamin negative balance. None of the subjects in the study had serum folate below abnormal values, i.e., less than 1.6 mg/ml, All subjects had RBC folate within normal range. Serum homocysteine showed inverse relationship with RBC folate and serum total cobalamin, TCI and TCII. Conclusions: 1) importance of using serum holotranscobalamin TCI and TCII as markers of cobalamin deficiency, 2) necessity to use documented quantitative components of dietary intake if strong comparisons are to be made among quantitative values of serum or plasma homocysteine, folate, cobalamin, and nutrients in food intake. C1 MT SINAI VET AFFAIRS MED CTR,NEW YORK,NY. BRONX VET AFFAIRS MED CTR,NEW YORK,NY. RP Flynn, MA (reprint author), UNIV MISSOURI,HLTH SCI CTR,DEPT FAMILY & COMMUNITY MED,DEPT STAT,1 HOSP DR M221,COLUMBIA,MO 65212, USA. NR 54 TC 20 Z9 21 U1 0 U2 1 PU AMER COLL NUTRITION PI NEW YORK PA C/O HOSP. JOINT DIS. 301 E. 17TH ST., NEW YORK, NY 10003 SN 0731-5724 J9 J AM COLL NUTR JI J. Am. Coll. Nutr. PD JUN PY 1997 VL 16 IS 3 BP 258 EP 267 PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA XB707 UT WOS:A1997XB70700012 PM 9176833 ER PT J AU Felsenfeld, AJ AF Felsenfeld, AJ TI Considerations for the treatment of secondary hyperparathyroidism in renal failure SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Article ID DIALYSATE CALCIUM-CONCENTRATION; PARATHYROID-HORMONE; INTRAVENOUS CALCITRIOL; HEMODIALYSIS-PATIENTS; UREMIC PATIENTS; BONE-DISEASE; PROSPECTIVE TRIAL; IONIZED CALCIUM; INTERMITTENT; HYPERPLASIA C1 UNIV CALIF LOS ANGELES,LOS ANGELES,CA. RP Felsenfeld, AJ (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,NEPHROL SECT 111L,DEPT MED,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 48 TC 46 Z9 48 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD JUN PY 1997 VL 8 IS 6 BP 993 EP 1004 PG 12 WC Urology & Nephrology SC Urology & Nephrology GA XD569 UT WOS:A1997XD56900018 PM 9189868 ER PT J AU Barnett, PG AF Barnett, PG TI Research without billing data - Econometric estimation of patient-specific costs SO MEDICAL CARE LA English DT Article DE hospital cost; long-term care cost; psychiatric care cost; ambulatory care cost; US Department of Veterans Affairs ID HOSPITAL COST AB OBJECTIVES. This article describes a method for computing the cost of care provided to individual patients in health care systems that do not routinely generate billing data, but gather information on patient utilization and total facility costs. METHODS. Aggregate data on cost and utilization were used to estimate how costs vary with characteristics of patients and facilities of the US Department of Veterans Affairs. A set of cost functions was estimated, taking advantage of the department-level organization of the data. Casemix measures were used to determine the costs of acute hospital and long-term care. RESULTS. Hospitalization for medical conditions cost an average of $5,642 per US Health Care Financing Administration diagnosis-related group weight; surgical hospitalizations cost $11,836. Nursing home care cost $197.33 per day, intermediate care cost $280.66 per day, psychiatric care cost $307.33 per day, and domiciliary care cost $111.84 per day. Outpatient visits cost an average of $90.36. These estimates include the cost of physician services. CONCLUSIONS. The econometric method presented here accounts for variation in resource use caused by casemix that is not reflected in length of stay and for the effects of medical education, research, facility size, and wage rates. Data on non-Veteran's Affairs hospital stays suggest that the method accounts for 40% of the variation in acute hospital care costs and is superior to cost estimates based on length of stay or diagnosis-related group weight alone. C1 US DEPT VET AFFAIRS,CTR COOPERAT STUDIES HLTH SERV,MENLO PK,CA. US DEPT VET AFFAIRS,HLTH SERV RES & DEV FIELD PROGRAM,MENLO PK,CA. STANFORD UNIV,DEPT HLTH RES & POLICY,STANFORD,CA 94305. NR 6 TC 18 Z9 18 U1 1 U2 2 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0025-7079 J9 MED CARE JI Med. Care PD JUN PY 1997 VL 35 IS 6 BP 553 EP 563 DI 10.1097/00005650-199706000-00002 PG 11 WC Health Care Sciences & Services; Health Policy & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA XD644 UT WOS:A1997XD64400002 PM 9191701 ER PT J AU Geraci, JM Ashton, CM Kuykendall, DH Johnson, ML Wu, L AF Geraci, JM Ashton, CM Kuykendall, DH Johnson, ML Wu, L TI International Classification of Diseases, 9th Revision, Clinical Modification codes in discharge abstracts are poor measures of complication occurrence in medical inpatients SO MEDICAL CARE LA English DT Article DE ICD-9-CM; administrative data bases; adverse events; complications ID ADMINISTRATIVE DATA; IDENTIFYING COMPLICATIONS; NOSOCOMIAL INFECTIONS; QUALITY ASSESSMENT; OUTCOMES RESEARCH; CARE; HOSPITALS; CLAIMS; SYSTEM; REIMBURSEMENT AB OBJECTIVES. The authors tested the ability of international Classification of Diseases, 9th Revision, Clinical Modification (ICD-9-CM) codes in discharge abstracts to identify medical inpatients who experienced an in-hospital complication, using complications identified through chart review as the gold standard. METHODS. TWO sets of ICD-9-CM codes were used: an inclusive set including many medical diagnoses that may also be coexistent complicating conditions on admission rather than complications and an exclusive set consisting primarily of ICD-9-CM-specified complication and adverse drug event codes. RESULTS. Neither set performed well as a diagnostic test for complication occurrence according to receiver operating characteristic analysis (ROC areas were 0.61 for the inclusive set and 0.55 for the exclusive set). Sensitivities of the ICD-9-CM codes for complications were 0.34 for the inclusive set and 0.14 for the exclusive set. Corresponding positive predictive values were 0.32 and 0.37, respectively. Sensitivities of code definitions for individual complications were generally poor, less than 0.5 in most cases. CONCLUSIONS. The authors conclude that ICD-9-CM codes in discharge abstracts are poor measures of complication occurrence. RP Geraci, JM (reprint author), BAYLOR COLL MED,DEPT MED,HOUSTON VET AFFAIRS MED CTR,MED SERV 3C,HOUSTON,TX 77030, USA. NR 28 TC 54 Z9 56 U1 1 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0025-7079 J9 MED CARE JI Med. Care PD JUN PY 1997 VL 35 IS 6 BP 589 EP 602 DI 10.1097/00005650-199706000-00005 PG 14 WC Health Care Sciences & Services; Health Policy & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA XD644 UT WOS:A1997XD64400005 PM 9191704 ER PT J AU Nagy, AK Walton, NY Treiman, DM AF Nagy, AK Walton, NY Treiman, DM TI Reduced cortical ecto-ATPase activity in rat brains during prolonged status epilepticus induced by sequential administration of lithium and pilocarpine SO MOLECULAR AND CHEMICAL NEUROPATHOLOGY LA English DT Article DE ecto-ATPase; synaptosomes; rat; lithium/pilecarpine; status epilepticus; subcellular brain fractions; Mg2+-dependent ATPase; Ca2+-dependent ATPase ID CENTRAL-NERVOUS-SYSTEM; ADENOSINE-TRIPHOSPHATASE-ACTIVITY; SYNAPTIC PLASMA-MEMBRANES; TORPEDO ELECTRIC ORGAN; SEIZURE-PRONE MICE; EXTRACELLULAR ATP; FOCAL EPILEPSY; SYNAPTOSOMES; RELEASE; DIPHOSPHOHYDROLASE AB Considerable evidence indicates that ATP, acting intracellularly or as a neurotransmitter, can influence nerve cell physiology in a variety of ways. Defects in the functioning of ATP-metabolizing enzymes could therefore lead to disturbances in neurotransmission and creation of sustained neuronal discharges characteristic of status epilepticus. in this study we investigated synaptosomal ATPase changes in rat brains during lithium/pilocarpine-induced status epilepticus. After 2 h of continuous electroencephalographic spiking, both Mg2+- and Ca2+-dependent ecto-ATPases were significantly decreased in freshly prepared synaptosomal preparations from the status rats. The intracellulary acting Ca2+M2+-ATPase (Ca-pump) was also decreased, but no changes occurred in synaptosomal Na+K+-ATPase activity. The difference between ecto-ATPase activities of the control and status rat brains was not affected by repeated freezing-thawing and lengthy storage. Possible involvement of reduced synaptosomal divalent cation-dependent ATPases in the pathophysiology of status epilepticus is discussed. C1 W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA 90095 USA. RP Nagy, AK (reprint author), UNIV CALIF LOS ANGELES, SCH MED, DEPT NEUROL, 10833 LE CONTE AVE, LOS ANGELES, CA 90095 USA. NR 52 TC 21 Z9 21 U1 0 U2 0 PU HUMANA PRESS INC PI TOTOWA PA 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA SN 1044-7393 J9 MOL CHEM NEUROPATHOL JI Mol. Chem. Neuropathol. PD JUN PY 1997 VL 31 IS 2 BP 135 EP 147 DI 10.1007/BF02815238 PG 13 WC Neurosciences; Pathology SC Neurosciences & Neurology; Pathology GA XT683 UT WOS:A1997XT68300003 PM 9376020 ER PT J AU Haroutunian, V Greig, N Pei, XF Utsuki, T Gluck, R Acevedo, LD Davis, KL Wallace, WC AF Haroutunian, V Greig, N Pei, XF Utsuki, T Gluck, R Acevedo, LD Davis, KL Wallace, WC TI Pharmacological modulation of Alzheimer's beta-amyloid precursor protein levels in the CSF of rats with forebrain cholinergic system lesions SO MOLECULAR BRAIN RESEARCH LA English DT Article DE amyloid beta-protein; amyloid beta-protein, precursor; rat; Alzheimer's disease; cerebrospinal fluid; cerebral cortex; basal nucleus of Meynert; acetylcholine; acetylcholinesterase; acetylcholinesterase inhibitor; butyrylcholinesterase; receptor, muscarinic; DFP; phenserine; in vivo ID INDUCED LEARNING IMPAIRMENT; 14-UNIT T-MAZE; ORAL PHYSOSTIGMINE; SENILE DEMENTIA; NUCLEUS BASALIS; CEREBRAL-CORTEX; AGED RATS; DISEASE; MEMORY; CHOLINESTERASE AB Abnormal deposition and accumulation of Alzheimer's amyloid beta-protein (A beta) and degeneration of forebrain cholinergic neurons are among the principal features of Alzheimer's disease. Studies in rat model systems have shown that forebrain cholinergic deficits are accompanied by induction of cortical beta-amyloid precursor protein (beta-APP) mRNAs and increased levels of secreted beta-APP in the CSF. The studies reported here determined whether the CSF levels of secreted beta-APP could be altered pharmacologically. In different experiments, rats with lesions of the forebrain cholinergic system received injections of vehicle, a muscarinic receptor antagonist scopolamine, or one of two cholinesterase inhibitors - diisopropyl phosphorofluoridate (DFP) or phenserine. Scopolamine was administered to determine whether the levels of beta-APP in the CSF could be increased by anticholinergic agents. The cholinesterase inhibitors were administered to determine whether the forebrain cholinergic system lesion-induced increases in CSF beta-APP could be reduced by cholinergic augmentation. Scopolamine administration led to a significant increase in the CSF levels of secreted beta-APP in sham-lesioned rats. Phenserine, a novel, reversible acetyl-selective cholinesterase inhibitor, significantly decreased the levels of secreted beta-APP in the CSF of forebrain cholinergic system-lesioned rats whereas DFP, a relatively non-specific cholinesterase inhibitor, failed to affect CSF levels of secreted beta-APP. These results suggest that the levels of secreted beta-APP in the CSF can be pharmacologically modulated but that this modulation is dependent upon the status of the forebrain cholinergic system and the pharmacological properties of the drugs used to influence it. C1 BRONX VET ADM MED CTR,NEW YORK,NY. MT SINAI SCH MED,DEPT PSYCHIAT,NEW YORK,NY. NIA,NEUROSCI LAB,BETHESDA,MD 20892. NIA,MOL & CELLULAR BIOL PROGRAM,MOL PHYSIOL & GENET SECT,BETHESDA,MD 20892. NR 59 TC 61 Z9 61 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-328X J9 MOL BRAIN RES JI Mol. Brain Res. PD JUN PY 1997 VL 46 IS 1-2 BP 161 EP 168 DI 10.1016/S0169-328X(96)00297-5 PG 8 WC Neurosciences SC Neurosciences & Neurology GA XA506 UT WOS:A1997XA50600019 ER PT J AU Shaner, A Roberts, LJ Eckman, TA Tucker, DE Tsuang, JW Wilkins, JN Mintz, J AF Shaner, A Roberts, LJ Eckman, TA Tucker, DE Tsuang, JW Wilkins, JN Mintz, J TI Monetary reinforcement of abstinence from cocaine among mentally ill patients with cocaine dependence SO PSYCHIATRIC SERVICES LA English DT Article; Proceedings Paper CT 58th Annual Meeting of the College-on-Problems-of-Drug-Dependence CY JUN 22-27, 1996 CL SAN JUAN, PR SP Coll Problems Drug Dependence ID ABUSERS AB Objective: The study investigated whether contingency management could reduce cocaine use by patients with schizophrenia. Methods: An A-B-A research design, with two-month baseline, intervention, and follow-up phases, was used to study two homeless, treatment-resistant male outpatients with DSM-III-R diagnoses of schizophrenia and cocaine dependence. During the intervention phase, subjects provided daily urine specimens for testing for the cocaine metabolite benzoylecgonine (BE) and received $25 for each negative test. Concentrations of BE and metabolites of other illicit drugs were assayed twice a week to determine the amount of drug use in addition to frequency. Analysis of variance was used to compare drug use during the three study phases. Results: During the intervention, the proportion of tests positive for cocaine was lower for both subjects. Mean urinary concentrations of BE were significantly lower during the intervention than during the baseline. Conclusions: These results suggest that modest monetary reinforcement of abstinence may decrease cocaine use among cocaine-dependent patients with schizophrenia. C1 UNIV CALIF LOS ANGELES, SCH MED, DEPT PSYCHIAT & BIOBEHAV SCI, LOS ANGELES, CA 90024 USA. RP W LOS ANGELES VET AFFAIRS MED CTR, 11301 WILSHIRE BLVD OOMH, LOS ANGELES, CA 90073 USA. FU NIMH NIH HHS [MH 30911] NR 13 TC 62 Z9 62 U1 1 U2 3 PU AMER PSYCHIATRIC PUBLISHING, INC PI ARLINGTON PA 1000 WILSON BOULEVARD, STE 1825, ARLINGTON, VA 22209-3901 USA SN 1075-2730 EI 1557-9700 J9 PSYCHIAT SERV JI Psychiatr. Serv. PD JUN PY 1997 VL 48 IS 6 BP 807 EP 810 PG 4 WC Health Policy & Services; Public, Environmental & Occupational Health; Psychiatry SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Psychiatry GA XB452 UT WOS:A1997XB45200011 PM 9175190 ER PT J AU Arita, S Thompson, K Stein, E Matsuda, N Cochrum, K Jemtrud, S Une, S Kawahara, T Shevlin, L Trieu, C Mullen, Y AF Arita, S Thompson, K Stein, E Matsuda, N Cochrum, K Jemtrud, S Une, S Kawahara, T Shevlin, L Trieu, C Mullen, Y TI In vitro activation of human lymphocytes by crude and purified alginates SO TRANSPLANTATION PROCEEDINGS LA English DT Article; Proceedings Paper CT International Congress of the Transplant-Society CY SEP 29-OCT 02, 1996 CL MIAMI BEACH, FL SP Transplantat Soc C1 UNIV CALIF LOS ANGELES,DEPT SURG,HUMAN ISLET PROGRAM,LOS ANGELES,CA 90024. RP Arita, S (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,DEPT SURG,HUMAN ISLET PROGRAM,11301 WILSHIRE BLVD,BLDG 304,LOS ANGELES,CA 90073, USA. NR 0 TC 3 Z9 3 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0041-1345 J9 TRANSPLANT P JI Transplant. Proc. PD JUN PY 1997 VL 29 IS 4 BP 2125 EP 2125 DI 10.1016/S0041-1345(97)00258-3 PG 1 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA XE833 UT WOS:A1997XE83300098 PM 9193553 ER PT J AU Teichman, JMH Rogenes, VJ Barber, DB AF Teichman, JMH Rogenes, VJ Barber, DB TI The Malone antegrade continence enema combined with urinary diversion in adult neurogenic patients: Early results SO UROLOGY LA English DT Article ID SPINAL-CORD INJURY; FECAL INCONTINENCE; CONSTIPATION AB Objectives. Patients with neurogenic voiding dysfunction often have coexisting neurogenic bowel problems. Impaired bowel evacuation is a cause of major morbidity and impaired lifestyle for these patients. The Malone antegrade continence enema (ACE) performed synchronously with a urinary continence procedure has been successful in pediatric patients. We report early experience combining the ACE with a urinary continence procedure in adult neurogenic patients. Methods. Adult patients with neurogenic voiding dysfunction and impaired bowel evacuation refractory to conservative management underwent a urinary continence procedure synchronously with an ACE. Results. Two patients have undergone the procedure. One patient chose a continent catheterizable supravesical bladder augmentation, whereas the other patient chose an ileal conduit. Both patients had a separate appendiceal stoma for their ACE. Both patients are continent of stool at their appendiceal stoma and per rectum. Both patients have stabilized their urinary tracts. Complications were minimal. Conclusions. The ACE may benefit adult patients with impaired bowel evacuation and may be combined with a urinary continence procedure. Further study of the ACE is warranted. (C) 1997, Elsevier Science Inc. C1 UNIV TEXAS,HLTH SCI CTR,SPINAL CORD INJURY SERV,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RP Teichman, JMH (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT REHABIL MED,DIV UROL,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. NR 17 TC 3 Z9 3 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0090-4295 J9 UROLOGY JI UROLOGY PD JUN PY 1997 VL 49 IS 6 BP 963 EP 967 DI 10.1016/S0090-4295(97)00087-3 PG 5 WC Urology & Nephrology SC Urology & Nephrology GA XC721 UT WOS:A1997XC72100034 PM 9187713 ER PT J AU McGough, P Back, AL Colley, J AF McGough, P Back, AL Colley, J TI Physician-assisted suicide - Finding common ground SO WESTERN JOURNAL OF MEDICINE LA English DT Article; Proceedings Paper CT Comprehensive Care of the Terminally Ill: The Northern California Consensus Development Conference for Guidelines on Aid-in-Dying CY SEP, 1996 CL STANFORD UNIV CTR BIOMED ETH, STANNFORD, CA SP Stanford Univ, Ctr Biomed Eth HO STANFORD UNIV CTR BIOMED ETH ID PATIENT REQUESTS; EUTHANASIA; DEATH; MANAGEMENT AB In Washington state, practicing physicians have been forced to confront the emotional, complex issue of physician-assisted suicide sooner than physicians elsewhere in the US.(1-12) The Washington State Medical Association has struggled at length with the issue and ultimately delineated a policy on safeguards for physician-assisted suicide. The Washington experience may prove instructive to other professional physician organizations even before the US Supreme Court rules on the issue. C1 VA PUGET SOUND HLTH CARE SYST,SEATTLE,WA. UNIV WASHINGTON,SEATTLE,WA 98195. RP McGough, P (reprint author), WASHINGTON STATE MED ASSOC END LIFE TASK FORCE,2033 6TH AVE,SUITE 1100,SEATTLE,WA 98121, USA. NR 26 TC 0 Z9 0 U1 0 U2 0 PU CARDEN JENNINGS PUBL CO LTD PI CHARLOTTESVILLE PA BLAKE CTR, STE 200, 1224 W MAIN ST, CHARLOTTESVILLE, VA 22903 SN 0093-0415 J9 WESTERN J MED JI West. J. Med. PD JUN PY 1997 VL 166 IS 6 BP 394 EP 397 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA XG751 UT WOS:A1997XG75100005 PM 9217451 ER PT J AU Sayin, U Rutecki, PA AF Sayin, U Rutecki, PA TI Effects of pilocarpine on paired pulse inhibition in the CA3 region of the rat hippocampus SO BRAIN RESEARCH LA English DT Article DE alveus; Str radiatum; epilepsy; muscarinic receptor; gamma-aminobutyric acid (GABA); inhibition; status epilepticus ID SPONTANEOUS RECURRENT SEIZURES; GABA-MEDIATED INHIBITION; DENTATE GYRUS; GABAERGIC INTERNEURONS; SYNAPTIC TRANSMISSION; EXPERIMENTAL-MODEL; PERFORANT PATH; FASCIA-DENTATA; EPILEPSY; DEPRESSION AB Pilocarpine (PILO), a muscarinic agonist, produces status epilepticus when administered to rats in vivo and induces interictal or ictal patterns of epileptiform activity in rat hippocampal slices. We investigated the effects of PILO (10 mu M) on paired pulse inhibition (PPI) in the CA3 region of rat hippocampal slices. PPI was assessed by stimulating either the alveus or str. radiatum and recording the extracellular response from str. pyramidale of CA3. The evoked population spike following the second stimulus was compared to the first. PILO was bath applied for 1 h and then washed out to assess acute and long lasting effects. PILO decreased the amplitude of evoked population spikes measured in CA3. PPI following alveus stimulation was not affected by PILO; however, a significant loss of PPI at 15 and 30 ms interpulse intervals occurred following str. radiatum stimulation in the presence of PILO and 5 mM [K+](0) artificial cerebrospinal fluid (ACSF). The decrease in PPI at the 15 ms interval persisted following wash-out of PILO. PILO in 7.5 mM [K+](0) ACSF produced epileptiform activity and a resultant long lasting loss of PPI that followed str. radiatum stimulation. This effect was not observed following epileptiform activity produced by 7.5 mM [K+](0) alone, suggesting that the loss of PPI was due to PILO. Because str. radiatum-evoked PPI was selectively impaired, PILO appears to preferentially decreased feed-forward inhibition. The more dramatic loss of PPI following exposure to PILO and high [K+](0) may represent the first steps in the development of chronic seizures that results from PILO-induced status epilepticus in rats. C1 UNIV WISCONSIN,DEPT NEUROSURG,MADISON,WI 53706. UNIV WISCONSIN,NEUROSCI TRAINING PROGRAM,MADISON,WI 53706. RP Sayin, U (reprint author), UNIV WISCONSIN,WILLIAM S MIDDLETON MEM VET ADM HOSP,DEPT NEUROL,600 HIGHLAND AVE,MADISON,WI 53792, USA. FU NINDS NIH HHS [NS 28580] NR 42 TC 7 Z9 7 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD MAY 30 PY 1997 VL 758 IS 1-2 BP 136 EP 142 DI 10.1016/S0006-8993(97)00198-4 PG 7 WC Neurosciences SC Neurosciences & Neurology GA XE606 UT WOS:A1997XE60600017 PM 9203542 ER PT J AU Belisle, JT Vissa, VD Sievert, T Takayama, K Brennan, PJ Besra, GS AF Belisle, JT Vissa, VD Sievert, T Takayama, K Brennan, PJ Besra, GS TI Role of the major antigen of Mycobacterium tuberculosis in cell wall biogenesis SO SCIENCE LA English DT Article ID CYCLOPROPANATED MYCOLIC ACIDS; PROTEINS; IDENTIFICATION; BIOSYNTHESIS; ETHAMBUTOL; SEQUENCE; COMPLEX; TARGET AB The dominant exported proteins and protective antigens of Mycobacterium tuberculosis are a triad of related gene products called the antigen 85 (Ag85) complex. Each has also been implicated in disease pathogenesis through its fibronectin-binding capacities, A carboxylesterase domain was found within the amino acid sequences of Ag85A, B, and C, and each protein acted as a mycolyltransferase involved in the final stages of mycobacterial cell wall assembly, as shown by direct enzyme assay and site-directed mutagenesis. Furthermore, the use of an antagonist (6-azido-6-deoxy-alpha,alpha'-trehalose) of this activity demonstrates that these proteins are essential and potential targets for new antimycobacterial drugs. C1 UNIV WISCONSIN, WILLIAM S MIDDLETON MEM VET HOSP, MYCOBACTERIOL RES LAB, MADISON, WI 53705 USA. RP COLORADO STATE UNIV, DEPT MICROBIOL, FT COLLINS, CO 80523 USA. RI Belisle, John/B-8944-2017 OI Belisle, John/0000-0002-2539-2798; Besra, Gurdyal/0000-0002-5605-0395 FU NIAID NIH HHS [AI-35220, AI-18357, AI-38087] NR 32 TC 428 Z9 464 U1 6 U2 41 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 EI 1095-9203 J9 SCIENCE JI Science PD MAY 30 PY 1997 VL 276 IS 5317 BP 1420 EP 1422 DI 10.1126/science.276.5317.1420 PG 3 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA XB533 UT WOS:A1997XB53300051 PM 9162010 ER PT J AU Wong, H Yang, D Hill, JS Davis, RC Nikazy, J Schotz, MC AF Wong, H Yang, D Hill, JS Davis, RC Nikazy, J Schotz, MC TI A molecular biology-based approach to resolve the subunit orientation of lipoprotein lipase SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID RECEPTOR-RELATED PROTEIN; HEPATIC LIPASE; TERMINAL DOMAIN; HEPARIN-BINDING; ENZYME-ACTIVITY; MUTAGENESIS; IDENTIFICATION; INACTIVATION; CATABOLISM; RESIDUES AB The subunit orientation of a dimeric enzyme influences the mechanism of action and function, To determine the subunit arrangement of lipoprotein lipase (LPL), a molecular biology-based approach was initiated, An eight amino acid linker region was engineered between two LPL monomers and expressed in COS-7 cells, The resultant tandem-repeat molecule (LPLTR) was lipolytically active and had kinetic parameters, salt inhibition, cofactor-dependent activity, heparin-binding characteristics, and a functional unit size very similar to the expressed native human enzyme, By these criteria, LPLTR was the functional equivalent of native LPL. Considering the length of the linker peptide (no more than 24 Angstrom), monomers in the tethered molecule were restricted to a head-to-tail subunit arrangement, Since LPLTR demonstrated native enzyme-like properties while constrained to this subunit arrangement, these results provide the first compelling evidence that native LPL monomers are arranged in a head-to-tail subunit orientation within the active dimer, Thus, LPL function in physiology, lipolysis, and binding to cell-surface components must now be addressed with this subunit orientation in mind, The utility of the tandem-repeat approach to resolve the subunit arrangement of an obligate dimer has been demonstrated with LPL and could be generalized for use with other oligomeric enzymes. C1 UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90024. RP Wong, H (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,LIPID RES LAB,BLDG 113,ROOM 312,LOS ANGELES,CA 90073, USA. FU NHLBI NIH HHS [HL28481, P01 HL028481] NR 33 TC 49 Z9 51 U1 0 U2 5 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAY 27 PY 1997 VL 94 IS 11 BP 5594 EP 5598 DI 10.1073/pnas.94.11.5594 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA XB711 UT WOS:A1997XB71100022 PM 9159117 ER PT J AU Byrd, TF AF Byrd, TF TI Tumor necrosis factor alpha (TNF alpha) promotes growth of virulent Mycobacterium tuberculosis in human monocytes - Iron-mediated growth suppression is correlated with decreased release of TNF alpha from iron-treated infected monocytes SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article; Proceedings Paper CT Annual Meeting of the Infectious-Diseases-Society-of-America CY OCT 06-10, 1994 CL ORLANDO, FL SP Infect Dis Soc Amer DE transferrin; lactoferrin; polymorphonuclear leukocyte; interferon gamma; calcitriol ID ACTIVATED HUMAN-MONOCYTES; TRANSFERRIN RECEPTOR EXPRESSION; INTERFERON-GAMMA; INTRACELLULAR MULTIPLICATION; LEGIONELLA-PNEUMOPHILA; HUMAN MACROPHAGES; POLYMORPHONUCLEAR LEUKOCYTES; MONONUCLEAR PHAGOCYTES; SCHISTOSOMA-MANSONI; IFN-GAMMA AB The human immune response to Mycobacterium tuberculosis is not well characterized, To better understand the cellular immune response to tuberculosis, a human mononuclear phagocyte culture system using a low-infecting inoculum of M. tuberculosis to mimic in vivo conditions was developed, Using this system, monocytes treated with IFN gamma/TNF alpha/calcitriol (CytD) were permissive for the growth of virulent M. tuberculosis. In the presence of iron, however, these monocytes suppressed the growth of M. tuberculosis, The enhanced permissiveness of CytD-preincubated monocytes was found to be due to TNF alpha, however, the ability of iron to suppress M. tuberculosis growth also required preincubation with TNF alpha Iron-mediated growth suppression was correlated with selective suppression of TNF alpha release from infected monocytes, In addition, removal of TNF alpha from CytD-treated monocytes 2 d after infection mimicked the suppressive effect of iron, suggesting that iron may also be decreasing monocyte sensitivity to exogenously added TNF alpha. In the absence of iron, permissive, CytD-treated monocytes formed large infected cellular aggregates, With iron treatment, aggregation was suppressed, suggesting that the iron-suppressive effect on M. tuberculosis growth may be related to suppression of monocyte aggregation and diminished cell-to-cell spread of M. tuberculosis, The results of this study indicate that TNF alpha preincubation is required for human monocytes to exert an iron-mediated suppressive effect on M. tuberculosis growth. In the absence of iron, however, the continued presence of TNF alpha has a growth-promoting effect on M. tuberculosis in human monocytes. Iron may be an important early modulator of M. tuberculosis growth via its effects on TNF alpha. C1 UNIV CALIF LOS ANGELES,SCH MED,W LOS ANGELES VET AFFAIRS MED CTR,DEPT MED,DIV INFECT DIS,LOS ANGELES,CA 90073. FU NIAID NIH HHS [AI-93-03] NR 66 TC 69 Z9 70 U1 0 U2 0 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD MAY 15 PY 1997 VL 99 IS 10 BP 2518 EP 2529 DI 10.1172/JCI119436 PG 12 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA XB226 UT WOS:A1997XB22600032 PM 9153296 ER PT J AU Yu, EY Siegal, JA Meyer, GE Ji, XR Ni, XZ Brawer, MK AF Yu, EY Siegal, JA Meyer, GE Ji, XR Ni, XZ Brawer, MK TI Histologic differences in benign prostrate hyperplasia between Chinese and American men SO PROSTATE LA English DT Article DE morphometry; Chinese; American; BPH ID PROSTATIC HYPERPLASIA; CANCER AB BACKGROUND. This investigation sought to determine morphologic differences between Chinese and Americans with symptomatic benign prostatic hyperplasia (BPH), in an admixture of stroma, epithelium, and luminal spaces. METHODS. Adjacent sections of simple prostatectomy specimens from China and the U.S. were stained to highlight the stroma and epithelium. Image analysis was performed on random fields. RESULTS. Chinese tissue had higher glandular densities (mean = 12.5 acini/mm(2) vs. 6.2 acini/mm(2); P < 0.0001), while American samples had higher percent stroma (mean = 66% vs. 51%; P = 0.0003). Mean luminal cell heights in the Chinese and American prostate acini were 11.7 and 19.0 microns, respectively (P < 0.0001). CONCLUSIONS. These data indicate significant histologic variation in BPH between Chinese and American men undergoing simple prostatectomy. These differences may provide a focus for investigating the epidemiological variation in clinical BPH and carcinoma between our two countries, and could have applications in clinical management of symptomatic BPH. (C) 1997 Wiley-Liss, Inc. C1 VA PUGET SOUND HLTH CARE SYST,UROL SECT,SEATTLE,WA 98108. AFFILIATED HOSP,QUINGDAO MED COLL,DEPT UROL,QINGDAO,PEOPLES R CHINA. UNIV WASHINGTON,DEPT UROL,SEATTLE,WA 98195. FU NIDDK NIH HHS [DK45761-01] NR 12 TC 8 Z9 9 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0270-4137 J9 PROSTATE JI Prostate PD MAY 15 PY 1997 VL 31 IS 3 BP 175 EP 179 PG 5 WC Endocrinology & Metabolism; Urology & Nephrology SC Endocrinology & Metabolism; Urology & Nephrology GA XA510 UT WOS:A1997XA51000005 PM 9167769 ER PT J AU Yan, SD Zhu, HJ Fu, J Yan, SF Roher, A Tourtellotte, WW Rajavashisth, T Chen, X Godman, GC Stern, D Schmidt, AM AF Yan, SD Zhu, HJ Fu, J Yan, SF Roher, A Tourtellotte, WW Rajavashisth, T Chen, X Godman, GC Stern, D Schmidt, AM TI Amyloid-beta peptide-receptor for advanced glycation endproduct interaction elicits neuronal expression of macrophage-colony stimulating factor: A proinflammatory pathway in Alzheimer disease SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE microglia; neurons; cerebrospinal fluid ID ADHESION MOLECULE-1 VCAM-1; HUMAN ENDOTHELIAL-CELLS; FACTOR-KAPPA-B; MICROGLIAL CELLS; NEURITE OUTGROWTH; PROTEIN TOXICITY; OXIDANT STRESS; IMMUNE-SYSTEM; END-PRODUCTS; ACTIVATION AB In Alzheimer disease (AD), neurons are thought to be subjected to the deleterious cytotoxic effects of activated microglia, We demonstrate that binding of amyloid-beta peptide (A beta) to neuronal Receptor for Advanced Glycation Endproduct (RAGE), a cell surface receptor for A beta, induces macrophage-colony stimulating factor (M-CSF) by an oxidant sensitive, nuclear factor kappa B-dependent pathway, AD brain shows increased neuronal expression of M-CSF in proximity to A beta deposits, and in cerebrospinal fluid from AD patients there was approximate to 5-fold increased M-CSF antigen (P < 0.01), compared with age-matched controls, M-CSF released by A beta-stimulated neurons interacts with its cognate receptor, c-fms, on microglia, thereby triggering chemotaxis, cell proliferation, increased expression of the macrophage scavenger receptor and apolipoprotein E, and enhanced survival of microglia exposed to A beta, consistent with pathologic findings in AD, These data delineate an inflammatory pathway triggered by engagement of A beta on neuronal RAGE, We suggest that M-CSF, thus generated, contributes to the pathogenesis of AD, and that M-CSF in cerebrospinal fluid might provide a means for monitoring neuronal perturbation at an early stage in AD. C1 COLUMBIA UNIV,COLL PHYS & SURG,DEPT SURG,NEW YORK,NY 10032. COLUMBIA UNIV,COLL PHYS & SURG,DEPT PHYSIOL,NEW YORK,NY 10032. COLUMBIA UNIV,COLL PHYS & SURG,DEPT MED,NEW YORK,NY 10032. SUN HLTH RES INST,HALDEMAN LAB ALZHEIMERS DIS RES,SUN CITY,AZ 85372. W LOS ANGELES VET AFFAIRS MED CTR,NATL NEUROL RES SPECIMEN BANK,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,LOS ANGELES CTY HARBOR MED CTR,SCH MED,TORRANCE,CA 90502. RP Yan, SD (reprint author), COLUMBIA UNIV,COLL PHYS & SURG,DEPT PATHOL,NEW YORK,NY 10032, USA. RI Fu, Jin/A-3510-2012 NR 53 TC 144 Z9 149 U1 0 U2 6 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAY 13 PY 1997 VL 94 IS 10 BP 5296 EP 5301 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA WZ257 UT WOS:A1997WZ25700086 ER PT J AU Shaker, JL Brickner, RC Findling, JW Kelly, TM Rapp, R Rizk, G Haddad, JG Schalch, DS Shenker, Y AF Shaker, JL Brickner, RC Findling, JW Kelly, TM Rapp, R Rizk, G Haddad, JG Schalch, DS Shenker, Y TI Hypocalcemia and skeletal disease as presenting features of celiac disease SO ARCHIVES OF INTERNAL MEDICINE LA English DT Article; Proceedings Paper CT 77th Annual Meeting of the Endocrine-Society CY JUN 14-17, 1995 CL WASHINGTON, DC SP Endocrine Soc, Minist Educ, Sci & Culture Japan, Dtsch ForschGemeinsch ID BONE-MINERAL DENSITY; VITAMIN-D DEFICIENCY; GLUTEN-FREE DIET; DIABETES-MELLITUS; OSTEOMALACIA; ENTEROPATHY; MECHANISM AB Objective: To describe 15 patients examined for hypo calcemia, skeletal disease, or both in whom the diagnosis of celiac disease was subsequently made. Design: Observational case series. Patients: Fifteen patients (7 women and 8 men) were examined for hypocalcemia (n=11), skeletal disease (n=3), or both (n=1). The diagnosis of celiac disease was subsequently made. The mean age of the patients was 62 years, and 11 patients were 60 years of age or older. Results: Four patients had no gastrointestinal symptoms, 7 patients had mild or intermittent gastrointestinal symptoms, and 4 patients had persistent diarrhea. Ten patients had experienced weight loss. The serum total alkaline phosphatase level was elevated in 10 of 15 patients, the parathyroid hormone level was elevated in all patients, and the urinary calcium level was low in all 6 of the patients tested. The level of 25-hydroxyvitamin D was frankly low in 4 patients, marginal in 8 patients, and normal in 3 patients. Bone mineral density was reduced in all 8 patients in whom it was measured. Conclusions: Celiac disease should be considered in patients with unexplained metabolic bone disease or hypocalcemia, especially because gastrointestinal symptoms may be absent or mild. Advanced age does not exclude the diagnosis of celiac disease. C1 ST JOSEPHS HOSP,DEPT MED,MILWAUKEE,WI. ST MARYS HOSP,DEPT MED,RACINE,WI. UNIV PENN,SCH MED,DEPT MED,PHILADELPHIA,PA 19104. UNIV WISCONSIN,SCH MED,DEPT MED,MADISON,WI. WILLIAM S MIDDLETON MEM VET ADM MED CTR,DEPT MED,MADISON,WI. RP Shaker, JL (reprint author), ST LUKES HOSP,DEPT MED,2901 W KINNICKINN RIVER PKWY,SUITE 503,MILWAUKEE,WI 53215, USA. NR 25 TC 26 Z9 26 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-9926 J9 ARCH INTERN MED JI Arch. Intern. Med. PD MAY 12 PY 1997 VL 157 IS 9 BP 1013 EP 1016 DI 10.1001/archinte.157.9.1013 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA WX439 UT WOS:A1997WX43900010 PM 9140273 ER PT J AU Carabello, BA AF Carabello, BA TI Timing of valve replacement in aortic stenosis - Moving closer to perfection SO CIRCULATION LA English DT Editorial Material DE valves; editorials; aorta; stenosis ID SYMPTOMATIC PATIENTS; ASYMPTOMATIC PATIENTS; NATURAL-HISTORY; REGURGITATION C1 MED UNIV S CAROLINA,GAZES CARDIAC RES INST,CHARLESTON,SC 29425. RALPH H JOHNSON DEPT VET AFFAIRS,CHARLESTON,SC. RP Carabello, BA (reprint author), MED UNIV S CAROLINA,DEPT MED,DIV CARDIOL,171 ASHLEY AVE,CHARLESTON,SC 29425, USA. NR 14 TC 49 Z9 50 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD MAY 6 PY 1997 VL 95 IS 9 BP 2241 EP 2243 PG 3 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA WW865 UT WOS:A1997WW86500001 PM 9141998 ER PT J AU Allen, DN Gilbertson, MW vanKammen, DP Kelley, ME Gurklis, JA Barry, EJ AF Allen, DN Gilbertson, MW vanKammen, DP Kelley, ME Gurklis, JA Barry, EJ TI Chronic haloperidol treatment does not affect structure of attention in schizophrenia SO SCHIZOPHRENIA RESEARCH LA English DT Article DE attention; haloperidol; schizophrenia ID DISORDERS AB Results of a number of investigations indicate attention is a multifactorial construct composed of four distinct cognitive factors including focus-execute, sustain, encode and shift abilities. While investigators have partially or fully replicated this attentional structure in a number of clinical and nonclinical populations, no study has adequately examined the structure of attention in patients with schizophrenia who are not treated with antipsychotics. In this study, we examined the four-factor theory of attention in patients with schizophrenia while they were stabilized on haloperidol(with no adjunctive antiparkinsonian/anticholinergic medications) and again when they were approximately 3 weeks drug free. Standard neuropsychological measures were used to assess attentional functions. Principal components analyses (varimax rotation) of neuropsychological test scores in medicated and drug-free conditions indicated that four factors accounted for 84.2 and 91.8 of total variance in medicated and unmedicated conditions, respectively. Based on these results, it appears that: (1) haloperidol does not appreciably affect structure of the attentional system in patients with schizophrenia; (2) unmedicated patients with schizophrenia exhibit a similar structure of attention as both medicated patients and controls, suggesting that attentional structure is 'normal' in schizophrenia: and (3) the four-factor attention theory is a useful and valid paradigm for evaluating attention in patients with schizophrenia, regardless of medication status. C1 VET ADM MED CTR,MANCHESTER,NH 03104. UNIV PITTSBURGH,DEPT PSYCHIAT,PITTSBURGH,PA 15213. RP Allen, DN (reprint author), VA PITTSBURGH HLTH CARE SYST,HIGH DR DIV,7180 HIGHLAND DR,PITTSBURGH,PA 15206, USA. FU NIMH NIH HHS [R01 MH44-841] NR 25 TC 16 Z9 16 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD MAY 3 PY 1997 VL 25 IS 1 BP 53 EP 61 DI 10.1016/S0920-9964(97)00006-6 PG 9 WC Psychiatry SC Psychiatry GA WZ796 UT WOS:A1997WZ79600006 PM 9176927 ER PT J AU Steiner, J Kirsteins, L LaPaglia, N Lawrence, A Williams, D Emanuele, N Emanuele, M AF Steiner, J Kirsteins, L LaPaglia, N Lawrence, A Williams, D Emanuele, N Emanuele, M TI The effect of acute ethanol (EtOH) exposure on protein kinase C (PKC) activity in anterior pituitary SO ALCOHOL LA English DT Article DE ethanol (EtOH); protein kinase C (PKC); anterior pituitary ID GONADOTROPIN-RELEASING-HORMONE; MESSENGER-RNA LEVELS; FOLLICLE-STIMULATING-HORMONE; LUTEINIZING-HORMONE; SECRETION; RAT; CELLS; EXPRESSION; CALCIUM; ACTIVATORS AB Alterations in the protein kinase C (PKC) pathway may interupt anterior pituitary luteinizing hormone (LH) synthesis and/or secretion, which may impair normal reproductive function. Work by our laboratory and others has shown that EtOH has profound deleterious effects on the regulation of the hypothalamic-pituitary-gonadal (HPG) axis. The present study focuses on PKC translocation from the cytosol to the membrane of anterior pituitary after acute EtOH exposure. Serum levels of LH were measured at three time points (15, 30, and 90 min) after an TP injection of either saline or 3 g/kg EtOH in adult castrated male rats. LH levels dropped significantly (p < 0.03) in EtOH-injected compared to saline-injected control animals. In the same animals, EtOH significantly suppressed PKC localization at its active site at the pituitary cell membrane (p < 0.05). These findings suggest that the mechanism of EtOH's suppression of LH is mediated, at least in part, through a decrease in PKC translocation to the anterior pituitary cell membrane. (C) 1997 Elsevier Science Inc. C1 LOYOLA UNIV,STRITCH SCH MED,DIV RES DRUGS ABUSE,MAYWOOD,IL 60153. LOYOLA UNIV,STRITCH SCH MED,DEPT MED,MAYWOOD,IL 60153. US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,RES & MED SERV,HINES,IL 60141. RP Steiner, J (reprint author), LOYOLA UNIV,STRITCH SCH MED,DEPT MOL & CELLULAR BIOCHEM,PROGRAM MOL BIOL,2160 S 1ST AVE,MAYWOOD,IL 60153, USA. NR 23 TC 10 Z9 10 U1 1 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0741-8329 J9 ALCOHOL JI Alcohol PD MAY-JUN PY 1997 VL 14 IS 3 BP 209 EP 211 DI 10.1016/S0741-8329(96)00113-9 PG 3 WC Substance Abuse; Pharmacology & Pharmacy; Toxicology SC Substance Abuse; Pharmacology & Pharmacy; Toxicology GA WZ025 UT WOS:A1997WZ02500002 PM 9160797 ER PT J AU Harris, RA Mihic, SJ Brozowski, S Hadingham, K Whiting, PJ AF Harris, RA Mihic, SJ Brozowski, S Hadingham, K Whiting, PJ TI Ethanol, flunitrazepam, and pentobarbital modulation of GABA(A) receptors expressed in mammalian cells and Xenopus oocytes SO ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH LA English DT Article DE GABA; receptors; ethanol; oocytes; benzodiazepine ID GAMMA-AMINOBUTYRIC-ACID; PROTEIN-KINASE-C; A RECEPTORS; SUBUNIT; PHOSPHORYLATION; PHARMACOLOGY; CURRENTS; DESENSITIZATION; POTENTIATION; RESPONSES AB GABA(A) receptors composed of human alpha 1 beta 2 gamma 2L, alpha 1 beta 2 gamma 2S, alpha 1 beta 3 gamma 2S, alpha 6 beta 3 gamma 2S, and alpha 5 beta 3 gamma 3 subunits as well as bovine alpha 1 beta 1 gamma 2L and alpha 1 beta 1 subunits were stably expressed in mammalian L(tk(-)) cells and transiently expressed in Xenopus oocytes, Effects of muscimol, ethanol, flunitrazepam, and pentobarbital on receptor function were compared for the two expression systems using a Cl-36(-) flux assay for cells and an electrophysiological assay for oocytes. Muscimol activated all receptors in both expression systems but was more potent for L(tk(-)) cells than oocytes; this difference ranged from 2.6- to 26-fold, depending upon subunit composition, The most pronounced differences between receptors and expression systems were found for ethanol. In L(tk(-)) cells, low (5-50 mM) concentrations of ethanol potentiated muscimol responses only with receptors containing the gamma 2L subunit, In oocytes, concentrations of 30-100 mM produced small enhancements for most subunit combinations, Flunitrazepam enhanced muscimol responses for all receptors except alpha 6 beta 3 gamma 2S and alpha 1 beta 1, and this enhancement was similar for both expression systems, Pentobarbital also enhanced muscimol responses for all receptors, and this enhancement was similar for L(tk(-)) cells and oocytes, except for alpha 6 beta 3 gamma 2S where the pentobarbital enhancement was much greater in oocytes than cells, The alpha 6 beta 3 gamma 2S receptors were also distinct in that pentobarbital produced direct activation of chloride channels in both expression systems. Thus, the type of expression/assay system markedly affects the actions of ethanol on GABA(A) receptors and also influences the actions of muscimol and pentobarbital on this receptor, Differences between these expression systems may reflect posttranslational modifications of receptor subunits. C1 DENVER VET AFFAIRS MED CTR,DENVER,CO. MERCK SHARP & DOHME RES LABS,HARLOW,ESSEX,ENGLAND. RP Harris, RA (reprint author), UNIV COLORADO,HLTH SCI CTR,DEPT PHARMACOL C236,SCH MED,4200 E 9TH AVE,DENVER,CO 80262, USA. FU NIAAA NIH HHS [AA06399, AA03527] NR 41 TC 61 Z9 61 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0145-6008 J9 ALCOHOL CLIN EXP RES JI Alcoholism (NY) PD MAY PY 1997 VL 21 IS 3 BP 444 EP 451 PG 8 WC Substance Abuse SC Substance Abuse GA WZ645 UT WOS:A1997WZ64500011 PM 9161604 ER PT J AU Hutchinson, FN Jones, WJ AF Hutchinson, FN Jones, WJ TI A cost-effectiveness analysis of anemia screening before erythropoietin in patients with end-stage renal disease SO AMERICAN JOURNAL OF KIDNEY DISEASES LA English DT Article DE anemia; erythropoietin; end-stage renal disease; cost-effectiveness ID RECOMBINANT-HUMAN-ERYTHROPOIETIN; BIRTH-WEIGHT INFANTS; PARATHYROID-HORMONE; HEMODIALYSIS-PATIENTS; EARLY PREDICTION; SERUM; IRON; FAILURE; TRANSFUSION; RESISTANCE AB The treatment efficacy of erythropoietin (EPO) in end-stage renal disease (ESRD) can be limited by deficiencies of iron, folate, or vitamin B-12, by hyperparathyroidism, or by aluminum intoxication. Since EPO costs are significant, this study attempted to determine the cost-effectiveness of performing a panel of screening tests for anemia before starting EPO. Anemia screening was performed prospectively in 48 new-onset ESRD patients at the Ralph H. Johnson Veterans Affairs Medical Center before EPO treatment was started. Serum iron, transferrin, folate, vitamin B-12, parathyroid hormone, and aluminum levels were determined, and transferrin saturation (Tfsat) was calculated at the first dialysis session. At presentation for dialysis, the mean hematocrit was 0.264 +/- 0.036 and the mean blood urea nitrogen was 32 +/- 2 mmol/L. Eighteen patients (37.5%) had a serum iron level tower than 7 mu mol/L, suggesting iron deficiency. Twenty-five patients (52%) had Tfsat less than 0.20, consistent with overt iron deficiency. No patient was found to be vitamin B-12 deficient, to be aluminum intoxicated, or to have significant hyperparathyroidism. One patient had folate deficiency. A cost-effectiveness analysis was performed assuming that (1) EPO would be given at an average starting dose of 6,000 U/wk at a cost of $14/2,000 U of EPO; (2) that without screening 1 month would elapse before a poor response was identified; and (3) that the failure to treat aluminum intoxication and hyperparathyroidism or to replete iron, vitamin B-12, or folate deficiency would significantly impair the response to EPO. The Tfsat screen had a cost-effectiveness ratio of 0.2019, saving approximately $5.00 in EPO use for each dollar of test administration. All other screens had cost-effectiveness ratios greater than 1.0, indicating that their testing costs exceeded dollar savings in EPO use. In conclusion, iron deficiency is common in anemic patients starting dialysis, but other causes of anemia are not. It is imperative that current clinical practices be influenced by cost-effectiveness considerations. Given the cost of laboratory screens, and the relative ineffectiveness of the other screens examined here to identify factors known to impair the response to EPO, anemia screening before initiating EPC therapy should be limited to tests to identify iron deficiency. (C) 1997 by the National Kidney Foundation, Inc. C1 MED UNIV S CAROLINA,DEPT HLTH POLICY & ADM,CHARLESTON,SC 29425. RALPH H JOHNSON DEPT VET AFFAIRS MED CTR,CHARLESTON,SC. RP Hutchinson, FN (reprint author), MED UNIV S CAROLINA,DEPT MED,DIV NEPHROL,171 ASHLEY MED,CHARLESTON,SC 29425, USA. NR 25 TC 29 Z9 32 U1 1 U2 3 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0272-6386 J9 AM J KIDNEY DIS JI Am. J. Kidney Dis. PD MAY PY 1997 VL 29 IS 5 BP 651 EP 657 DI 10.1016/S0272-6386(97)90116-5 PG 7 WC Urology & Nephrology SC Urology & Nephrology GA WZ641 UT WOS:A1997WZ64100002 PM 9159297 ER PT J AU Aurang, K Spraggs, CF Jordan, C Lloyd, KCK AF Aurang, K Spraggs, CF Jordan, C Lloyd, KCK TI Role of gastrin/CCK-B receptors in meal-stimulated acid secretion in rats SO AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY LA English DT Article DE stomach; histamine; somatostatin; receptor antagonists ID HISTAMINE-RELEASE; CHOLECYSTOKININ RECEPTORS; SOMATOSTATIN SECRETION; STOMACH; ANTAGONISTS; PEPTONE; NEURONS; CCK; ACETYLCHOLINE; PENTAGASTRIN AB Gastrin is the principal hormonal mediator of gastric acid secretion. Using an in vivo, intact, anesthetized rat model, we studied the role of gastrin/cholecystokinin (CCK)-B receptors in regulating the release of histamine and somatostatin during intragastric stimulation of acid secretion during a peptone meal. In pylorus-ligated, adult male rats (each implanted with a gastric cannula and portal venous and splenic artery catheters), after a 30-min basal period, gastric acid secretion was stimulated for 90 min either by an intravenous infusion of gastrin-17 (15 mu g.kg(-1).h(-1)) or by extragastric titration of 5 mi 8% peptone meal at pH 5.5. Basal and stimulated acid outputs and portal venous plasma gastrin, histamine, and somatostatin concentrations were measured before and after close-arterial injection of a new, relatively selective, gastrin/CCK-B receptor antagonist GR143330X. GR143330X reduced basal acid output by 50% but not basal plasma gastrin, histamine, or somatostatin concentrations. GR143330X reduced gastrin-stimulated acid output by 80%, plasma histamine by 70%, and plasma somatostatin by 34%. During intragastric peptone meal stimulation GR143330X reduced the acid response by 42% during the 30- to 60-min period but not during the 60- to 90-min period. GR143330X reduced the plasma histamine response by 72 and 68%, and the plasma somatostatin response by 32 and 54% during the 30- to 60- and 60- to 90-min periods, respectively. GR143330X did not block the gastrin response to peptone at any time. These results indicate that GR143330X is an effective agent for blocking gastrin-stimulated acid secretion and histamine and somatostatin release in rats. Furthermore, we show for the first time in an intact, in vivo, anesthetized rat model that meal-stimulated activation of gastrin/CCK-B receptors stimulates acid secretion in part by regulating the release of histamine and somatostatin. C1 W LOS ANGELES VET AFFAIRS MED CTR, DEPT VET AFFAIRS, RES SERV, LOS ANGELES, CA 90073 USA. W LOS ANGELES VET AFFAIRS MED CTR, DEPT VET AFFAIRS, MED SERV, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT PHYSIOL, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT MED, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, CTR ULCER RES & EDUC, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, GASTROENTER BIOL CTR, LOS ANGELES, CA 90073 USA. GLAXO GRP RES LTD, RES & DEV, WARE SG12 0DP, HERTS, ENGLAND. NR 34 TC 10 Z9 10 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0193-1857 J9 AM J PHYSIOL-GASTR L JI Am. J. Physiol.-Gastroint. Liver Physiol. PD MAY PY 1997 VL 272 IS 5 BP G1243 EP G1248 PG 6 WC Gastroenterology & Hepatology; Physiology SC Gastroenterology & Hepatology; Physiology GA WZ327 UT WOS:A1997WZ32700038 ER PT J AU Lloyd, KCK Amirmoazzami, S Friedik, F Heynio, A Solomon, TE Walsh, JH AF Lloyd, KCK Amirmoazzami, S Friedik, F Heynio, A Solomon, TE Walsh, JH TI Candidate canine enterogastrones: acid inhibition before and after vagotomy SO AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY LA English DT Article DE peptide YY; cholecystokinin; secretin; neurotensin; glucagon-like peptide-1; oxyntomodulin; somatostatin; gastric inhibitory peptide; gastric acid secretion ID STIMULATED GASTRIC-ACID; DUODENAL-ULCER PATIENTS; PEPTIDE-YY; OLEIC-ACID; SOMATOSTATIN RELEASE; TRUNCAL VAGOTOMY; FAT INHIBITION; INTESTINAL FAT; SECRETION; DOGS AB The relative contributions of several gut-derived peptides as enterogastrones known to be released in response to a fatty meal and to inhibit acid secretion have not previously been compared directly. We determined the acid-inhibitory activities of increasing intravenous doses of several peptides before and after highly selective vagotomy (HSV) during intragastric titration of a peptone meal in dogs. Before HSV, threshold inhibitory doses of peptide YY (PYY), cholecystokinin (CCK), and secretin were 5, 7, and 10 pmol.kg(-1).h(-1), respectively, whereas neurotensin, glucagonlike peptide-1 (GLP-1), and oxyntomodulin failed to inhibit acid secretion at doses up to 1,000 pmol.kg(-1).h(-1). The calculated dose producing 50% acid inhibition (ID50) Of secretin (62 pmol.kg(-1).h(-1)) was one-half that of PW (128 pmol.kg(-1).h(-1)). Maximal (90%) acid inhibition was produced by 100 pmol.kg(-1).h(-1) secretin and 500 pmol.kg(-1).h(-1) PYY. The highest dose of CCK that did not cause vomiting (100 pmol.kg(-1).h(-1)) inhibited peptone-stimulated acid output by only 60%. After HSV, 500 pmol.kg(-1).h(-1) PYY and 200 pmol.kg(-1).h(-1) CCK failed to inhibit acid output by more than 50%. Threshold doses for inhibition by PW and CCK were 200 and 100 pmol.kg(-1).h(-1), respectively. Secretin remained a potent inhibitor after HSV, with an ID50 Of 80 pmol.kg(-1).h(-1) and a threshold dose of 10 pmol.kg(-1).h(-1). HSV also failed to affect inhibition caused by somatostatin. This study has shown that PYY and secretin are somewhat more potent and efficacious inhibitors of acid secretion than CCK but that all three peptides are far more active than GLP-1, neurotensin, and oxyntomodulin. PW and CCK inhibit acid secretion in large part through vagal innervation of the gastric fundus, but the inhibitory effects of secretin are independent of fundic vagal innervation. C1 W LOS ANGELES VET AFFAIRS MED CTR, DEPT VET AFFAIRS, RES SERV, LOS ANGELES, CA 90073 USA. W LOS ANGELES VET AFFAIRS MED CTR, DEPT VET AFFAIRS, MED SERV, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT PHYSIOL, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT MED, LOS ANGELES, CA 90073 USA. RP Lloyd, KCK (reprint author), UNIV CALIF LOS ANGELES, CURE VA WADSWORTH MED CTR, DIGEST DIS CORE CTR, BLDG 115, RM 115, LOS ANGELES, CA 90073 USA. FU NIDDK NIH HHS [DK-41301, DK-45752] NR 53 TC 9 Z9 9 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0193-1857 J9 AM J PHYSIOL-GASTR L JI Am. J. Physiol.-Gastroint. Liver Physiol. PD MAY PY 1997 VL 272 IS 5 BP G1236 EP G1242 PG 7 WC Gastroenterology & Hepatology; Physiology SC Gastroenterology & Hepatology; Physiology GA WZ327 UT WOS:A1997WZ32700037 PM 9176235 ER PT J AU Hunter, JC Escobedo, EM Wilson, AJ Hanel, DP ZinkBrody, GC Mann, FA AF Hunter, JC Escobedo, EM Wilson, AJ Hanel, DP ZinkBrody, GC Mann, FA TI MR imaging of clinically suspected scaphoid fractures SO AMERICAN JOURNAL OF ROENTGENOLOGY LA English DT Article; Proceedings Paper CT 96th Annual Meeting of the American-Roentgen-Ray-Society CY MAY 05-10, 1996 CL SAN DIEGO, CA SP Amer Roentgen Ray Soc ID OCCULT FRACTURES; BONE; CT; SCINTIGRAPHY AB OBJECTIVE. The purpose of this study was to evaluate the usefulness of MR imaging in revealing occult fractures in patients with clinically suspected acute scaphoid fractures who have normal or equivocal findings on radiographs. SUBJECTS AND METHODS. Thirty-six patients underwent MR imaging within 7 days of wrist injury. All had physical findings suggestive of scaphoid fracture. Coronal T1-weighted, short inversion time inversion recovery, and either T2-weighted or proton density-weighted fast spin-echo sequences with fat suppression were used. Follow-up radiographs were obtained at least 2 weeks after MR imaging whenever possible. All imaging studies were reviewed by two musculoskeletal radiologists. RESULTS. MR imaging revealed 22 occult fractures in 20 patients. Thirteen of these 22 fractures were in the scaphoid bone, and nine were in the distal radius. On MR images, 18 patients had no evidence of fracture. Follow-up radiographs were available in 14 of the 20 patients who had occult fracture revealed by MR imaging. Eleven of the 13 occult fractures of the scaphoid bone were followed up (two were lost to follow-up), and 10 of the 11 showed signs of healing. Five of the nine lesions of the distal radius were followed up, and three of these showed evidence of healing fracture. Three patients without MR evidence of a fracture had follow-up radiographs that showed no fracture. Three patients had findings consistent with bone contusion on MR images; in two patients, the contusion was associated with other fractures, and in one patient, the contusion was isolated. CONCLUSION. MR imaging can reveal occult wrist fractures when findings on radiographs are normal or equivocal. C1 UNIV WASHINGTON,HARBORVIEW MED CTR,DEPT ORTHOPAED SURG,SEATTLE,WA 98104. UNIV WASHINGTON,VET AFFAIRS PUGET SOUND HLTH CARE SYST,DEPT RADIOL,SEATTLE,WA 98108. UNIV WASHINGTON,HARBORVIEW MED CTR,DEPT ORTHOPAED SURG,SEATTLE,WA 98104. KAISER PERMANENTE HOSP,DEPT RADIOL,HAYWARD,CA 94545. RP Hunter, JC (reprint author), UNIV WASHINGTON,HARBORVIEW MED CTR,DEPT RADIOL,325 9TH AVE,BOC 359728,SEATTLE,WA 98104, USA. NR 33 TC 86 Z9 87 U1 0 U2 0 PU AMER ROENTGEN RAY SOC PI RESTON PA 1891 PRESTON WHITE DR, SUBSCRIPTION FULFILLMENT, RESTON, VA 22091 SN 0361-803X J9 AM J ROENTGENOL JI Am. J. Roentgenol. PD MAY PY 1997 VL 168 IS 5 BP 1287 EP 1293 PG 7 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA WV568 UT WOS:A1997WV56800032 PM 9129428 ER PT J AU Duerinckx, AJ Hayrapetian, A Melany, M Valentino, DJ Rahbar, D Kiszonas, M Franco, R Narin, SL Ragavendra, N Grant, EG AF Duerinckx, AJ Hayrapetian, A Melany, M Valentino, DJ Rahbar, D Kiszonas, M Franco, R Narin, SL Ragavendra, N Grant, EG TI Real-time sonographic video transfer using asynchronous transfer mode technology SO AMERICAN JOURNAL OF ROENTGENOLOGY LA English DT Article ID NETWORK C1 UNIV CALIF LOS ANGELES,CTR HLTH SCI,DEPT RADIOL,LOS ANGELES,CA 90095. GTE TEL OPERAT,THOUSAND OAKS,CA 91362. RP Duerinckx, AJ (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,SERV RADIOL,11301 WILSHIRE BLVD,MAIL ROUTE W114,MRI,BLDG 506,LOS ANGELES,CA 90073, USA. NR 9 TC 7 Z9 7 U1 0 U2 0 PU AMER ROENTGEN RAY SOC PI RESTON PA 1891 PRESTON WHITE DR, SUBSCRIPTION FULFILLMENT, RESTON, VA 22091 SN 0361-803X J9 AM J ROENTGENOL JI Am. J. Roentgenol. PD MAY PY 1997 VL 168 IS 5 BP 1353 EP 1355 PG 3 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA WV568 UT WOS:A1997WV56800047 PM 9129443 ER PT J AU Wallace, J Johnson, NM AF Wallace, J Johnson, NM TI Stereotactic needle core biopsy - Reply SO AMERICAN JOURNAL OF SURGERY LA English DT Letter RP Wallace, J (reprint author), PORTLAND VA MED CTR,SURG SERV,PORTLAND,OR 97207, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9610 J9 AM J SURG JI Am. J. Surg. PD MAY PY 1997 VL 173 IS 5 BP 453 EP 454 DI 10.1016/S0002-9610(97)80035-9 PG 2 WC Surgery SC Surgery GA XA201 UT WOS:A1997XA20100023 ER PT J AU Nguyen, MH Najvar, LK Yu, CY Graybill, JR AF Nguyen, MH Najvar, LK Yu, CY Graybill, JR TI Combination therapy with fluconazole and flucytosine in the murine model of Cryptococcal meningitis SO ANTIMICROBIAL AGENTS AND CHEMOTHERAPY LA English DT Article ID AMPHOTERICIN-B; AIDS AB This study elucidates the role of combined fluconazole and flucytosine as therapy for cryptacorcosis in the murine model of meningitis, Three strains of Cryptococus neoformans for which the range of fluconazole MICs was wide-2 mu g/ml (susceptible strain), 8 mu g/ml (moderately susceptible strain), and 32 mu g/ml (resistant strain ere used for infection, One day postinfection, the mice were randomized into eight treatment groups: placebo; flucytosine (40 mg/kg of body weight/day); fluconazole at 3 mg/kg/day (low dosage), 10 mg/kg/day !moderate dosage), or 10 mg/kg/day (high dosage); and combined flucytosine and fluconazole at low, moderate, or high doses of fluconazole. Three major findings were demonstrated: (i) correlation between the MICs for the isolates and the in vivo effectiveness of fluconazole as assessed by the reduction in cryptococcal brain burden, (iii a dose-response curve (a higher dose of fluconazole was significantly more efficacious than a lower dose [P < 0.001]), and (iii) synergism between fluconazole and flucytosine (therapy with a combination of fluconazole and flucytosine was superior to therapy with either agent alone [P < 0.01]). C1 VET ADM MED CTR,GAINESVILLE,FL 32602. UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RP Nguyen, MH (reprint author), UNIV FLORIDA,COLL MED,J HILLIS MILLER HLTH CTR,DEPT MED,DIV INFECT DIS,POB 100277,GAINESVILLE,FL 32610, USA. NR 11 TC 40 Z9 40 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0066-4804 J9 ANTIMICROB AGENTS CH JI Antimicrob. Agents Chemother. PD MAY PY 1997 VL 41 IS 5 BP 1120 EP 1123 PG 4 WC Microbiology; Pharmacology & Pharmacy SC Microbiology; Pharmacology & Pharmacy GA WX583 UT WOS:A1997WX58300042 PM 9145879 ER PT J AU Reite, M Sheeder, J Teale, P Adams, M Richardson, D Simon, J Jones, RH Rojas, DC AF Reite, M Sheeder, J Teale, P Adams, M Richardson, D Simon, J Jones, RH Rojas, DC TI Magnetic source imaging evidence of sex differences in cerebral lateralization in schizophrenia SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Article ID HUMAN AUDITORY-CORTEX; GENDER DIFFERENCES; FIRST-EPISODE; TEMPORAL-LOBE; TONOTOPIC ORGANIZATION; PLANUM TEMPORALE; BRAIN MORPHOLOGY; SOURCE LOCATION; ABNORMALITIES; ASYMMETRIES AB Background: It has been postulated that schizophrenia represents a disorder of anomalous cerebral lateralization. This study is a replication of earlier preliminary findings using a multichannel neuromagnetometer, suggesting altered lateralization in schizophrenia in male subjects, with an extension of the findings to female subjects. Methods: We used magnetoencephalography-based magnetic source imaging to estimate the intracranial location of the 100-millisecond latency auditory-evoked field component (M100) in both left and right hemispheres of 20 patients with paranoid schizophrenia and 20 controls without schizophrenia. Neuroanatomical data were obtained by means of magnetic resonance imaging, from which we segmented and computed volumes of both total brain and left and right superior temporal gyri. Results: Locations of M100 source were compatible with neuronal generators located in the transverse gyri of Heschl on the superior temporal gyri in both study groups; M100 sources were asymmetric in all the control subjects. The male patient subgroup exhibited significantly less asymmetry than the control group, while the female patient subgroup actually showed significantly more asymmetry. The male patient subgroup generally had smaller superior temporal gyri than the control group. No evidence of total brain volume differences was observed. Conclusions: Our findings support previous magnetoencephalography-based studies suggesting anomalous cerebral lateralization in schizophrenia. Further, in extending our studies to female patients, our data suggest that the nature of this anomaly is sex specific, a finding that, to our knowledge, has not previously been reported. C1 UNIV COLORADO, HLTH SCI CTR, DEPT RADIOL, DENVER, CO 80262 USA. UNIV COLORADO, HLTH SCI CTR, DEPT PREVENT MED & BIOMETR, DENVER, CO 80262 USA. DENVER VET AFFAIRS MED CTR, PSYCHIAT SERV, DENVER, CO USA. RP Reite, M (reprint author), UNIV COLORADO, HLTH SCI CTR, DEPT PSYCHIAT, BOX C268-68, 4200 E 9TH AVE, DENVER, CO 80262 USA. RI Rojas, Don/F-4296-2012 OI Rojas, Don/0000-0001-6560-9616; Sheeder, Jeanelle/0000-0002-4463-3569 FU NIMH NIH HHS [MH46335, MH47476] NR 76 TC 84 Z9 85 U1 1 U2 4 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60654-0946 USA SN 0003-990X J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD MAY PY 1997 VL 54 IS 5 BP 433 EP 440 PG 8 WC Psychiatry SC Psychiatry GA WY633 UT WOS:A1997WY63300006 PM 9152097 ER PT J AU Green, MF Nuechterlein, KH Breitmeyer, B AF Green, MF Nuechterlein, KH Breitmeyer, B TI Backward masking performance in unaffected siblings of schizophrenic patients - Evidence for a vulnerability indicator SO ARCHIVES OF GENERAL PSYCHIATRY LA English DT Article ID VISUAL MASKING; DISORDERS; DEFICITS; CHANNELS; SYMPTOM; MANIA AB Background: Visual masking is a procedure that is used to assess the earliest components of visual processing. In backward masking, the identification of an initial stimulus (the target) is disrupted by a later stimulus (the mask). The masking function can be divided into an early component (eg, up to about 60 ms) that reflects the involvement of sensory-perceptual processes, and a later component that reflects susceptibility to attentional disengagement as the mask diverts processing away from the representation of the target. Schizophrenic patients show anomalies on both masking components. It is not known whether backward masking deficits reflect enduring genetic vulnerability to schizophrenia. Methods: We assessed 32 unaffected siblings of schizophrenic patients and 52 normal control subjects on the early and late components of 4 masking conditions. The conditions differentially involved the sustained and transient visual pathways. Results: The unaffected siblings showed poorer overall performance than control subjects on the masking procedures. More specifically, siblings showed anomalies on the early, sensory-perceptual component, but not on the later, attentional disengagement component. Conclusions: The backward masking performance deficits that have been observed in schizophrenic patients appear to reflect enduring vulnerability to the disorder rather than only the symptoms of the illness. This vulnerability appears to be associated with early, sensory-perceptual processes. C1 UNIV CALIF LOS ANGELES,DEPT PSYCHOL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90024. UNIV HOUSTON,DEPT PSYCHOL,HOUSTON,TX. RP Green, MF (reprint author), UNIV CALIF LOS ANGELES,INST NEUROPSYCHIAT,DEPT PSYCHIAT & BIOBEHAV SCI,760 WESTWOOD PLAZA,C9-420,LOS ANGELES,CA 90024, USA. FU NIMH NIH HHS [MH-43292] NR 38 TC 103 Z9 105 U1 0 U2 1 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-990X J9 ARCH GEN PSYCHIAT JI Arch. Gen. Psychiatry PD MAY PY 1997 VL 54 IS 5 BP 465 EP 472 PG 8 WC Psychiatry SC Psychiatry GA WY633 UT WOS:A1997WY63300009 PM 9152100 ER PT J AU Chandrasekar, B Colston, JT Freeman, GL AF Chandrasekar, B Colston, JT Freeman, GL TI Induction of proinflammatory cytokine and antioxidant enzyme gene expression following brief myocardial ischaemia SO CLINICAL AND EXPERIMENTAL IMMUNOLOGY LA English DT Article DE reperfusion injury; ischaemia; proinflammatory cytokines; antioxidant enzymes; gene expression ID TUMOR-NECROSIS-FACTOR; MANGANESE SUPEROXIDE-DISMUTASE; NF-KAPPA-B; HEAT-SHOCK PROTEIN; FACTOR-ALPHA; STUNNED MYOCARDIUM; IONIZING-RADIATION; MESSENGER-RNA; INTERLEUKIN-1; ISCHEMIA AB The purpose of this study was to determine if proinflammatory cytokines are up-regulated during reperfusion following sublethal ischaemia, and whether concurrent up-regulation of antioxidant enzymes occurs. Open-chest rats were subjected to 15 min of ischaemia followed by 1 or 3 h reperfusion (R). Myocardium from the ischaemic zone showed a significantly higher (P < 0.01) generation of thiobarbituric acid-reactive substances at Ih and 3 h R. Northern blots showed a weak signal in controls for IL-6 mRNA (0.13 +/- 0.01); this was elevated to 0.68 +/- 0.12 at 1 h and 0.69 +/- 0.10 at 3 h R. Neither IL-1 beta nor tumour necrosis factor-alpha (TNF-alpha) were detectable in controls. IL-1 beta rose to 0.78 +/- 0.07 at 1 h and 0.51 +/- 0.08 at 3 h R, and TNF-alpha rose to 0.69 +/- 0.10 at 1 h and 0.38 +/- 0.15 at 3 h R. Western blotting showed no signals in the control, but readily detectable signals at 1 h R; these remained high (IL-6) or decreased (IL-1 beta and TNF-alpha) at 3 h R. mRNA analysis for antioxidant enzymes revealed a weak signal in controls for catalase (CAT; 0.16 +/- 0.08), glutathione peroxidase (GSH-Px; 0.15 +/- 0.06) and superoxide dismutase (SOD; 0.21 +/- 0.05). After 1 h R, levels increased significantly for CAT (0.46 +/- 0.10; P < 0.025) and GSH-Px (0.51 +/- 0.13; P < 0.01), but remained similar to controls for SOD (0.26 +/- 0.15). At 3 h R the mRNA levels were significantly elevated for the three enzymes (CAT 0.48 +/- 0.13; GSH-Px 0.47 +/- 0.10; SOD 0.54 +/- 0.08). We conclude that mRNA for proinflammatory cytokines is expressed early in reperfusion, and that the proteins are present in heart tissue. Also, reperfusion is associated with rapid expression of genes for antioxidant enzymes, which may enhance reactive oxygen intermediate (ROI) scavenging. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV CARDIOVASC,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RI Ain, Kenneth/A-5179-2012 OI Ain, Kenneth/0000-0002-2668-934X NR 38 TC 49 Z9 50 U1 0 U2 0 PU BLACKWELL SCIENCE LTD PI OXFORD PA P O BOX 88, OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0NE SN 0009-9104 J9 CLIN EXP IMMUNOL JI Clin. Exp. Immunol. PD MAY PY 1997 VL 108 IS 2 BP 346 EP 351 DI 10.1046/j.1365-2249.1997.d01-1017.x PG 6 WC Immunology SC Immunology GA WY467 UT WOS:A1997WY46700026 PM 9158109 ER PT J AU Pfaller, MA Rex, JH Rinaldi, MG AF Pfaller, MA Rex, JH Rinaldi, MG TI Antifungal susceptibility testing: Technical advances and potential clinical applications SO CLINICAL INFECTIOUS DISEASES LA English DT Article ID IN-VITRO SUSCEPTIBILITY; FLUCONAZOLE-RESISTANT CANDIDA; HUMAN-IMMUNODEFICIENCY-VIRUS; BROTH MICRODILUTION METHOD; AMPHOTERICIN-B; COLLABORATIVE EVALUATION; MULTICENTER EVALUATION; MACRODILUTION METHOD; TORULOPSIS-GLABRATA; NATIONAL-COMMITTEE AB The clinical application of in vitro antifungal susceptibility testing has been limited by a lack of reproducibility and uncertain clinical relevance. As a result of several collaborative studies, the National Committee for Clinical Laboratory Standards (NCCLS) has proposed a standardized antifungal susceptibility test method, NCCLS M27-T. More convenient, user-friendly methods (microdilution broth and stable gradient technology) have been evaluated, and the potential for a similar process with a disk diffusion method is apparent, Adaptation of the standard method for susceptibility testing of filamentous fungi appears promising, The existence of a standardized method facilitates meaningful analysis of studies addressing the issue of clinical relevance of antifungal susceptibility testing, Correlation of MICs with clinical response to therapy is beginning to emerge, most notably in relation to fluconazole and itraconazole therapy for oropharyngeal candidiasis associated with infection with the human immunodeficiency virus. This accumulated experience with antifungal susceptibility testing allows us to provide several specific recommendations for antifungal susceptibility testing in the clinical laboratory, Application of this developing technology to new antifungal agents and other disease states will enhance our ability to effectively deal with the emerging problem of fungal infection. C1 UNIV TEXAS, SCH MED,CTR STUDY EMERGING & REEMERGING PATHOGENS, DEPT INTERNAL MED,DIV INFECT DIS, HOUSTON, TX USA. UNIV TEXAS, HLTH SCI CTR, DEPT PATHOL, AUDIE L MURPHY MEM VET HOSP, SAN ANTONIO, TX 78284 USA. RP Pfaller, MA (reprint author), UNIV IOWA, COLL MED, DEPT PATHOL, 273 MRC, IOWA CITY, IA 52242 USA. NR 75 TC 83 Z9 87 U1 0 U2 2 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD MAY PY 1997 VL 24 IS 5 BP 776 EP 784 PG 9 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA WX257 UT WOS:A1997WX25700004 PM 9142769 ER PT J AU Ali, MZ Goetz, MB AF Ali, MZ Goetz, MB TI A meta-analysis of the relative efficacy and toxicity of single daily dosing versus multiple daily dosing of aminoglycosides SO CLINICAL INFECTIOUS DISEASES LA English DT Article ID GRAM-NEGATIVE INFECTIONS; FEBRILE NEUTROPENIC PATIENTS; DAILY NETILMICIN REGIMENS; URINARY-TRACT INFECTION; SERIOUS INFECTIONS; COMPARATIVE TRIAL; GENTAMICIN-NEPHROTOXICITY; PSEUDOMONAS-AERUGINOSA; CONVENTIONAL SCHEDULES; CORTICAL ACCUMULATION AB We performed a meta-analysis of the efficacy and toxicity of single daily dosing (SDD) vs. multiple daily dosing of aminoglycosides and summarized the results of the four previously published meta-analyses on this subject. Our analysis showed that the overall clinical response rate favored SDD therapy (mean difference, + 3.06%; 95% confidence limit [CL], + 0.17% to + 5.95%; P = .04). However, we found no significant difference in the overall microbiological response rates (mean difference. + 1.25%; 95% CL, - 0.40% to + 2.89%) or in the clinical response rates (mean difference, + 0.62%; 95% CL, - 2.48% to + 3.7%) when patients who received adjunctive antimicrobial therapy were excluded from the analysis. No significant differences were found in the incidences of nephrotoxicity, ototoxicity, or vestibular toxicity; the summary differences in the rates of these toxicities were - 0.18% (95% CL, - 2.17% to + 1.81%), + 1.38% (95% CL, - 0.99% to + 3.75%), and - 3.05% (95% CL, - 10.69% to + 4.59%), respectively. These results are similar to those of the previously published meta-analyses. C1 W LOS ANGELES VET AFFAIRS MED CTR,DEPT MED,LOS ANGELES,CA 90073. WILKES BARRE VET AFFAIRS MED CTR,DEPT MED,WILKES BARRE,PA. OI Goetz, Matthew/0000-0003-4542-992X NR 88 TC 154 Z9 163 U1 2 U2 3 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD MAY PY 1997 VL 24 IS 5 BP 796 EP 809 PG 14 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA WX257 UT WOS:A1997WX25700007 PM 9142772 ER PT J AU Barnes, CJ Hardman, WE Cameron, IL Lee, M AF Barnes, CJ Hardman, WE Cameron, IL Lee, M TI Aspirin, but not sodium salicylate, indomethacin, or nabumetone, reversibly suppresses 1,2-dimethylhydrazine-induced colonic aberrant crypt foci in rats SO DIGESTIVE DISEASES AND SCIENCES LA English DT Article DE aspirin; sodium salicylate; indomethacin; nabumetone; prostaglandin E-2; aberrant crypt foci; colon; rat; carcinogenesis ID NONSTEROIDAL ANTIINFLAMMATORY DRUGS; DIFFERENTIAL INHIBITION; CARCINOGENESIS; CYCLOOXYGENASE-2; SYNTHASE-2; CANCER; TUMORS; MICE AB The aim of this study was to determine if selective inhibition of cyclooxygenase isozymes affects the initiation of carcinogen-induced colon cancer using aberrant crypt foci (ACF) as a surrogate biomarker, Male Sprague-Dawley rats (18 per group) were given single subcutaneous injections of saline (4 ml/kg), aspirin (50 mg/kg body wt) sodium salicylate (50 mg/kg), indomethacin (4 mg/kg), nabumetone (100 mg/kg), or 16,16-dimethyl-prostaglandin E-2 (50 mg/kg) for three days. On day 4, 12 rats per group were given a subcutaneous injection of 1,2-dimethylhydrazine (12 mg base/kg body wt) and six rats per group received vehicle alone (4 ml/kg) every week for eight weeks, after which drug treatment ceased. Control and six carcinogen-treated rats per group were killed at this time and the remaining six rats per group killed 22 weeks later. Colons were scored for ACF number and size. Only aspirin caused a significant reduction in total ACF and ACF formation at the early time point, but at the later time, there were no significant differences between groups, ACF from all treatment groups increased in size at similar rates at both time points, Thus, only aspirin demonstrated a significant, although reversible, suppression of carcinogen-induced ACF. Possible mechanisms of action and the clinical implications of aspirin chemoprevention are discussed. C1 UNIV TEXAS,HLTH SCI CTR,DIV GASTROENTEROL & NUTR,DEPT MED,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT CELLULAR & STRUCT BIOL,SAN ANTONIO,TX 78284. VET AFFAIRS MED CTR,SAN ANTONIO,TX 78284. NR 33 TC 17 Z9 18 U1 0 U2 0 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0163-2116 J9 DIGEST DIS SCI JI Dig. Dis. Sci. PD MAY PY 1997 VL 42 IS 5 BP 920 EP 926 DI 10.1023/A:1018812430512 PG 7 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WY084 UT WOS:A1997WY08400008 PM 9149043 ER PT J AU Martin, MG Wu, SV Walsh, JH AF Martin, MG Wu, SV Walsh, JH TI Ontogenetic development and distribution of antibody transport and Fc receptor mRNA expression in rat intestine SO DIGESTIVE DISEASES AND SCIENCES LA English DT Article DE absorption; lactation; immunity ID CLASS-I ANTIGENS; SUCKLING RATS; NEONATAL RAT; IMMUNOGLOBULIN TRANSFER; EPITHELIAL-CELLS; IGG; FETAL; MICE; MHC; TRANSMISSION AB The intestine of the suckling rat has the unique capacity of absorbing immunoglobulins from maternal milk. We investigated intestinal Fc receptor mRNA expression and the absorption of orally administered antibodies to delineate the ontogeny and tissue specificity of this transport system, Duodenal expression of Fc receptor mRNA was at maximum levels between 1 and 19 days of age, but was not detectable during fetal life and in animals after weaning. Along the horizontal axis of the intestine, FcRn mRNA expression was maximum in the proximal duodenum and declined gradually in distal bowel. Similarly, absorption of orally administered antibody was low shortly after birth, but reached maximum levels at 14 days of age, By the time of weaning, antibody uptake had almost completely ceased, These data further delineate the temporal and spatial nature of the intestinal immunoglobulin transport system, and represent additional examples of how the intestinal Fc receptor is transcriptionally regulated. C1 UNIV CALIF LOS ANGELES, SCH MED, DEPT MED, DIV GASTROENTEROL, LOS ANGELES, CA 90024 USA. W LOS ANGELES VET AFFAIRS MED CTR, CURE, DIGEST DIS RES CTR, LOS ANGELES, CA 90073 USA. RP UNIV CALIF LOS ANGELES, SCH MED, DEPT PEDIAT, DIV GASTROENTEROL, LOS ANGELES, CA 90095 USA. FU NICHD NIH HHS [HD-22297]; NIDCR NIH HHS [DE-10054]; NIDDK NIH HHS [DK-07180] NR 45 TC 59 Z9 66 U1 0 U2 1 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0163-2116 EI 1573-2568 J9 DIGEST DIS SCI JI Dig. Dis. Sci. PD MAY PY 1997 VL 42 IS 5 BP 1062 EP 1069 DI 10.1023/A:1018853506830 PG 8 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WY084 UT WOS:A1997WY08400028 PM 9149063 ER PT J AU Ehrman, RN Robbins, SJ Cornish, JW AF Ehrman, RN Robbins, SJ Cornish, JW TI Comparing self-reported cocaine use with repeated urine tests in outpatient cocaine abusers SO EXPERIMENTAL AND CLINICAL PSYCHOPHARMACOLOGY LA English DT Article ID ADDICTION SEVERITY INDEX; DRUG-USE; HEROIN USE; VALIDITY; RELIABILITY; HAIR AB Sixty-one participants in outpatient therapy for cocaine dependence provided urine samples and self-reports of cocaine use 3 times a week for 4 weeks. Participants later gave a retrospective self-report of cocaine use for the month on the Addiction Severity Index (ASI). Comparisons with urine test values revealed substantial underreporting of cocaine use on both measures, and results from the 2 forms of self-report were only imperfectly correlated, More participants admitted to at least I cocaine use episode on The ASI than on the repeated self-reports, but the repeated reports provided a more accurate index of the relative frequency of cocaine use during the month, Self-reports can enhance cocaine Use detection when urines are infrequently collected and can help determine whether consecutive positive urine samples represent elevated metabolite levels from a single drug use episode. However, self-reports cannot substitute for regular urine sampling. C1 VET AFFAIRS MED CTR,PSYCHIAT SERV,PHILADELPHIA,PA. RP Ehrman, RN (reprint author), UNIV PENN,TREATMENT RES CTR,3900 CHESTNUT ST,PHILADELPHIA,PA 19104, USA. FU NIDA NIH HHS [DA03008] NR 15 TC 28 Z9 28 U1 1 U2 2 PU AMER PSYCHOLOGICAL ASSOC PI WASHINGTON PA 750 FIRST ST NE, WASHINGTON, DC 20002-4242 SN 1064-1297 J9 EXP CLIN PSYCHOPHARM JI Exp. Clin. Psychopharmacol. PD MAY PY 1997 VL 5 IS 2 BP 150 EP 156 DI 10.1037/1064-1297.5.2.150 PG 7 WC Psychology, Biological; Psychology, Clinical; Pharmacology & Pharmacy; Psychiatry SC Psychology; Pharmacology & Pharmacy; Psychiatry GA WY838 UT WOS:A1997WY83800009 PM 9234052 ER PT J AU Richardson, A AF Richardson, A TI Untitled SO EXPERIMENTAL GERONTOLOGY LA English DT Letter ID CULTURE; CELLS RP Richardson, A (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,CTR GERIATR RES EDUC & CLIN,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. NR 4 TC 0 Z9 0 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0531-5565 J9 EXP GERONTOL JI Exp. Gerontol. PD MAY-JUN PY 1997 VL 32 IS 3 BP 349 EP 350 DI 10.1016/S0531-5565(96)00127-1 PG 2 WC Geriatrics & Gerontology SC Geriatrics & Gerontology GA XA774 UT WOS:A1997XA77400015 ER PT J AU Jarvis, BW Qureshi, N AF Jarvis, BW Qureshi, N TI Inhibition of lipopolysaccharide-induced transcription factor Sp1 binding by spectrally pure diphosphoryl lipid A from Rhodobacter sphaeroides, protein kinase inhibitor H-8, and dexamethasone SO INFECTION AND IMMUNITY LA English DT Article ID NF-KAPPA-B; CELLS; CD14; IMMUNOSUPPRESSION; GLUCOCORTICOIDS; ACTIVATION; ATCC-17023; INDUCTION; ENDOTOXIN; RECEPTORS AB The transcription factor Sp1 plays a crucial role in the monocyte-specific expression of CD14, a binding site (or putative receptor) for lipopolysaccharide (LPS) complexes with LPS-binding protein (LBP). By using RAW 264.7 macrophages treated with spectrally pure deep-rough-chemotype hexa-acyl LPS from Escherichia coli D31m4, three inhibitors were found to block the binding activity of transcription factor Sp1, as measured by electrophoretic mobility shift assays. These inhibitors were diphosphoryl lipid A from Rhodobacter sphaeroides (10 u mu g/ml); the isoquinoline-sulfonamide H-8 (10 and 100 mu M), which is through to be a cGMP-dependent protein kinase inhibitor; and the anti-inflammatory agent dexamethasone (10 mu M). C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,MYCOBACTERIOL LAB,MADISON,WI 53705. UNIV WISCONSIN,DEPT BACTERIOL,MADISON,WI 53705. FU NIGMS NIH HHS [GM-50870] NR 28 TC 15 Z9 16 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD MAY PY 1997 VL 65 IS 5 BP 1640 EP 1643 PG 4 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA WW398 UT WOS:A1997WW39800010 PM 9125541 ER PT J AU Levin, RM Hypolite, JA Broderick, GA AF Levin, RM Hypolite, JA Broderick, GA TI Evidence for a role of intracellular-calcium release in nitric oxide-stimulated relaxation of the rabbit corpus cavernosum SO JOURNAL OF ANDROLOGY LA English DT Article DE calcium; erection; thapsigargin; ryanodine ID SMOOTH-MUSCLE; SARCOPLASMIC-RETICULUM; CONTRACTILE RESPONSE; FIELD STIMULATION; PENILE ERECTION; THAPSIGARGIN; RYANODINE; NEUROTRANSMISSION; MODULATION; MEMBRANES AB Erection is mediated by relaxation of the smooth muscle elements within the sinusoids of the corpus cavernosum. Although cavernosal relaxation can be mediated by a variety of mechanisms including purinergic stimulation, prostoglandins, and beta-adrenergic stimulation the major mechanism involves the stimulated release-of nitric oxide (NO) and subsequent relaxation of the corporal smooth muscle. Experimentally, NO can be released both by direct stimulation of NO-containing nerves (using field stimulation) and indirectly via cholinergic stimulation of NO release from the endothelium (using bethanechol). Preliminary studies have indicated that NO release and/or NO-stimulated relaxation of corporal smooth muscle is an active process involving both an increase in cytosolic calcium and an increase in metabolic energy utilization. Ryanodine is a pharmacological agent that can inhibit calcium-stimulated calcium release from the sarcoplasmic reticulum. The results of the current study demonstrated that ryanodine inhibited both field-stimulated relaxation and bethanechol-stimulated relaxation but did not affect relaxation induced by adenosine triphosphate (ATP) or nitroprusside. These studies strongly support the hypothesis that NO-stimulated relaxation is mediated, in part, by calcium release from the sarcoplasmic reticulum through ryanodine-sensitive channels. C1 STRATTON VET AFFAIRS MED CTR, ALBANY, NY USA. UNIV PENN, SCH MED, DIV UROL, PHILADELPHIA, PA 19104 USA. VET AFFAIRS MED CTR, PHILADELPHIA, PA USA. RP Levin, RM (reprint author), ALBANY COLL PHARM, DEPT BIOL SCI, 106 NEW SCOTLAND AVE, ALBANY, NY 12208 USA. FU NIDDK NIH HHS [1RO1 DK 33559]; PHS HHS [R0-3 46162] NR 27 TC 9 Z9 9 U1 0 U2 0 PU AMER SOC ANDROLOGY, INC PI LAWRENCE PA C/O ALLEN PRESS, INC PO BOX 368, LAWRENCE, KS 66044 SN 0196-3635 J9 J ANDROL JI J. Androl. PD MAY-JUN PY 1997 VL 18 IS 3 BP 246 EP 249 PG 4 WC Andrology SC Endocrinology & Metabolism GA XF813 UT WOS:A1997XF81300004 PM 9203051 ER PT J AU Silva, JA Leong, GB Rhodes, LJ Weinstock, R AF Silva, JA Leong, GB Rhodes, LJ Weinstock, R TI A new variant of ''subjective'' delusional misidentification associated with aggression SO JOURNAL OF FORENSIC SCIENCES LA English DT Article DE forensic science; forensic psychiatry; delusional misidentification; aggression; violence; psychosis; visual perception; chromosomal abnormalities ID POSITRON EMISSION TOMOGRAPHY; FACE-PROCESSING IMPAIRMENTS; CAPGRAS SYNDROME; GLUCOSE-METABOLISM; SCHIZOPHRENIA; SUBSTITUTION; PERSPECTIVE; DIAGNOSIS; ATROPHY; SELF AB Delusional misidentification syndromes are psychotic conditions in which the affected individual experiences delusions of radical change concerning the identity of others and/or of the self. These syndromes may lead to aggression including serious violence toward others. In this article, we describe and analyze in derail an aggressive individual who suffered from a delusion that physical and psychological replicas of himself existed. We specifically analyze the link between the patient's subjective misidentification delusion and his resulting aggression. Both the roles of phenomenology and biology of delusional misidentification are evaluated as potential contributors of aggression. C1 UNIV MISSOURI,SCH MED,COLUMBIA,MO. HARRY S TRUMAN MEM VET HOSP,COLUMBIA,MO 65201. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. RP Silva, JA (reprint author), S TEXAS VET HLTH CARE SYST,AUDIE L MURPHY DIV,PSYCHIAT SERV 116A,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. NR 63 TC 4 Z9 4 U1 0 U2 1 PU AMER SOC TESTING MATERIALS PI W CONSHOHOCKEN PA 100 BARR HARBOR DR, W CONSHOHOCKEN, PA 19428-2959 SN 0022-1198 J9 J FORENSIC SCI JI J. Forensic Sci. PD MAY PY 1997 VL 42 IS 3 BP 406 EP 410 PG 5 WC Medicine, Legal SC Legal Medicine GA WX108 UT WOS:A1997WX10800011 PM 9144929 ER PT J AU Leaf, DA Kobashigawa, J Gleeson, M Laks, H AF Leaf, DA Kobashigawa, J Gleeson, M Laks, H TI Defining obesity in patients undergoing orthotopic heart transplantation: Body mass index versus percent body fat SO JOURNAL OF HEART AND LUNG TRANSPLANTATION LA English DT Article ID HYPERLIPIDEMIA; PREDICTORS AB We examined body mass index (BMI) as a proxy for percent body fat among 26 men and women successfully undergoing orthotopic heart transplantation. Percent body fat was determined by use of bioelectrical impedance techniques. We found that, although BMI was well correlated with percent body fat (r = 0.58, p < 0.01), use of a BMI of greater than 27 kg/m(2) to define obesity potentially misclassified patients when compared with defining obesity as a percentage of body fat as both greater than 30% (BMI = 9 of 26 patients vs percent body fat = 6 of 26 patients) and greater than 40% (BMI = 9 of 26 versus percent body fat = 1 of 9). We conclude that percent body fat measurements are more methodologically appropriate means for defining obesity among these patients. RP Leaf, DA (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,DIV GEN INTERNAL MED 691 111G,WILSHIRE & SAWTELLE BLVD,LOS ANGELES,CA 90073, USA. NR 9 TC 4 Z9 4 U1 1 U2 1 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 1053-2498 J9 J HEART LUNG TRANSPL JI J. Heart Lung Transplant. PD MAY PY 1997 VL 16 IS 5 BP 563 EP 565 PG 3 WC Cardiac & Cardiovascular Systems; Respiratory System; Surgery; Transplantation SC Cardiovascular System & Cardiology; Respiratory System; Surgery; Transplantation GA XB106 UT WOS:A1997XB10600013 PM 9171276 ER PT J AU Hardin, TC Najvar, LK Rizzo, J Fothergill, AW Rinaldi, MG Graybill, JR AF Hardin, TC Najvar, LK Rizzo, J Fothergill, AW Rinaldi, MG Graybill, JR TI Discrepancy between in vitro and in vivo antifungal activity of albendazole SO JOURNAL OF MEDICAL AND VETERINARY MYCOLOGY LA English DT Article; Proceedings Paper CT 35th Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC) CY SEP 17-22, 1995 CL SAN FRANCISCO, CA SP Amer Soc Microbiol DE antifungal; albendazole; Candida albicans; Cryptococcus neoformans ID BENZIMIDAZOLES AB Albendazole has in vitro activity against Cryptococcus neoformans and reduced in vitro activity for albendazole when compared with Candida albicans. The major metabolite of albendazole, albendazole sulphoxide showed no in vitro activity against isolates of either fungus. Immunocompetent mice infected intravenously (IV) with C. albicans were treated with albendazole doses of 20-600 mg kg(-1) per day in noble agar or sesame oil for per oral (PO) administration, or 80 mg kg(-1) per day in DMSO for intraperitoneal (IP) and IV administration for 10 days, and were observed for survival. Mice infected with C. neoformans intracranially received albendazole in daily doses of 600 mg kg(-1) prepared in DMSO (IF) or peanut butter/rat chow (PO) for 10 days and were observed for survival. Mortality was not different between the treated and control animals in any study. Plasma samples from uninfected mice dosed with similar formulations and doses of albendazole were analysed by HPLC for albendazole and albendazole sulphoxide. No albendazole could be detected in any sample, while concentrations of albendazole sulphoxide (286-8697 ng ml(-1)) were observed in all samples. These data suggest that the absence of in vivo activity for albendazole is due to rapid conversion to the inactive albendazole sulphoxide metabolite. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX 78284. S TEXAS VET HLTH CARE SYST,AUDIE L MURPHY DIV,SAN ANTONIO,TX 78284. RP Hardin, TC (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT PHARMACOL,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. FU PHS HHS [N0 1-A1-25141] NR 8 TC 4 Z9 5 U1 0 U2 0 PU BLACKWELL SCIENCE LTD PI OXFORD PA P O BOX 88, OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0NE SN 0268-1218 J9 J MED VET MYCOL JI J. Med. Vet. Mycol. PD MAY-JUN PY 1997 VL 35 IS 3 BP 153 EP 158 PG 6 WC Mycology SC Mycology GA XG192 UT WOS:A1997XG19200001 PM 9229330 ER PT J AU Rajendran, JG Jacobson, AF AF Rajendran, JG Jacobson, AF TI Long-term outcome in patients with low probability lung scans: Effect of the modified PIOPED criteria. SO JOURNAL OF NUCLEAR MEDICINE LA English DT Meeting Abstract C1 UNIV WASHINGTON,SEATTLE,WA 98195. VA PUGET SOUND HLTH CARE SYST,SEATTLE,WA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SOC NUCLEAR MEDICINE INC PI RESTON PA 1850 SAMUEL MORSE DR, RESTON, VA 20190-5316 SN 0161-5505 J9 J NUCL MED JI J. Nucl. Med. PD MAY PY 1997 VL 38 IS 5 SU S BP 458 EP 458 PG 1 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA WY980 UT WOS:A1997WY98000458 ER PT J AU Silverman, DHS Munakata, JA Mandelkern, MA Hoh, CK Mayer, EA AF Silverman, DHS Munakata, JA Mandelkern, MA Hoh, CK Mayer, EA TI Comparing regional cerebral activity associated with visceral stimuli in male and female patients. SO JOURNAL OF NUCLEAR MEDICINE LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES,MED CTR,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SOC NUCLEAR MEDICINE INC PI RESTON PA 1850 SAMUEL MORSE DR, RESTON, VA 20190-5316 SN 0161-5505 J9 J NUCL MED JI J. Nucl. Med. PD MAY PY 1997 VL 38 IS 5 SU S BP 1150 EP 1150 PG 1 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA WY980 UT WOS:A1997WY98001056 ER PT J AU Jacobson, AF Hammersmith, SM AF Jacobson, AF Hammersmith, SM TI Value of the baseline bone scan in patients with newly diagnosed prostate cancer and low prostate specific antigen level. SO JOURNAL OF NUCLEAR MEDICINE LA English DT Meeting Abstract C1 VA PUGET SOUND HLTH CARE SYST,SEATTLE,WA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SOC NUCLEAR MEDICINE INC PI RESTON PA 1850 SAMUEL MORSE DR, RESTON, VA 20190-5316 SN 0161-5505 J9 J NUCL MED JI J. Nucl. Med. PD MAY PY 1997 VL 38 IS 5 SU S BP 1262 EP 1262 PG 1 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA WY980 UT WOS:A1997WY98001167 ER PT J AU Klein, S Kinney, J Jeejeebhoy, K Alpers, D Hellerstein, M Murray, M Twomey, P Bistrian, B Bothe, A Heitkemper, M Hubbard, V Schnackenberg, D Bernstein, E Hambidge, M Heymsfield, S IretonJones, C Wolfson, M Jacobs, D Keithley, J Meguid, M Pingleton, S Kopple, J Koretz, R Shulman, R Wilmore, D Driscoll, D Fleming, CR Greene, H Sitrin, M Herrmann, V Lipman, T Mock, D Borum, P Shronts, E AF Klein, S Kinney, J Jeejeebhoy, K Alpers, D Hellerstein, M Murray, M Twomey, P Bistrian, B Bothe, A Heitkemper, M Hubbard, V Schnackenberg, D Bernstein, E Hambidge, M Heymsfield, S IretonJones, C Wolfson, M Jacobs, D Keithley, J Meguid, M Pingleton, S Kopple, J Koretz, R Shulman, R Wilmore, D Driscoll, D Fleming, CR Greene, H Sitrin, M Herrmann, V Lipman, T Mock, D Borum, P Shronts, E TI Nutrition support in clinical practice: Review of published data and recommendations for future research directions SO JOURNAL OF PARENTERAL AND ENTERAL NUTRITION LA English DT Review ID TOTAL PARENTERAL-NUTRITION; ACQUIRED-IMMUNODEFICIENCY-SYNDROME; SHORT BOWEL SYNDROME; CHAIN AMINO-ACIDS; BONE-MARROW TRANSPLANTATION; SEVERE ACUTE-PANCREATITIS; ACUTE-RENAL-FAILURE; DOUBLE-BLIND TRIAL; PERCUTANEOUS ENDOSCOPIC GASTROSTOMY; SUBJECTIVE GLOBAL ASSESSMENT AB In the last 30 years, marked advances in enteral feeding techniques, venous access, and enteral and parenteral nutrient formulations have made it possible to provide nutrition support to almost all patients. Despite the abundant medical Literature and widespread use of nutritional therapy, many areas of nutrition support remain controversial. Therefore, the leadership at the National Institutes of Health, The American Society for Parenteral and Enteral Nutrition, and The American Society for Clinical Nutrition convened an advisory committee to perform a critical review of the current medical literature evaluating the clinical use of nutrition support; the goal was to assess our current body of knowledge and to identify the issues that deserve further investigation. The panel was divided into five groups to evaluate the following areas: nutrition assessment, nutrition support in patients with gastrointestinal diseases, nutrition support in wasting diseases, nutrition support in critically ill patients, and perioperative nutrition support. The findings from each group are summarized in this report. This document is not meant to establish practice guidelines for nutrition support. The use of nutritional therapy requires a careful integration of data from pertinent clinical trials, clinical expertise in the illness or injury being treated, clinical expertise in nutritional therapy, and input from the patient and his/her family. C1 HARVARD UNIV,MED CTR,BETH ISRAEL DEACONESS MED CTR,BOSTON,MA 02115. UNIV CHICAGO,MED CTR,CHICAGO,IL 60637. UNIV WASHINGTON,SEATTLE,WA 98195. NIDDKD,BETHESDA,MD 20892. ASCN,BETHESDA,MD. ASPEN,SILVER SPRING,MD. ROCKEFELLER UNIV,NEW YORK,NY 10021. ST MICHAELS HOSP,TORONTO,ON M5B 1W8,CANADA. UNIV COLORADO,MED CTR,DENVER,CO 80202. ST LUKES ROOSEVELT HOSP,NEW YORK,NY 10025. CORAM HEALTHCARE,CARROLLTON,TX. BAXTER HEALTHCARE CORP,MCGAW PK,IL. SAN FRANCISCO VET ADM MED CTR,SAN FRANCISCO,CA. BRIGHAM & WOMENS HOSP,BOSTON,MA 02115. RUSH PRESBYTERIAN ST LUKES MED CTR,CHICAGO,IL. SUNY HLTH SCI CTR,SYRACUSE,NY 13210. UNIV KANSAS,MED CTR,KANSAS CITY,KS 66103. MAYO CLIN,ROCHESTER,MN. HARBOR UCLA MED CTR,TORRANCE,CA 90509. OLIVE VIEW UCLA MED CTR,SYLMAR,CA 91342. BAYLOR COLL MED,HOUSTON,TX 77030. BRIGHAM & WOMENS HOSP,BOSTON,MA 02115. BETH ISRAEL DEACONESS MED CTR,BOSTON,MA. MAYO CLIN,JACKSONVILLE,FL 32224. SLIM FAST FOOD CO,W PALM BEACH,FL. UNIV CHICAGO,CHICAGO,IL 60637. UNIV CALIF BERKELEY,BERKELEY,CA 94720. ST LOUIS UNIV,SCH MED,ST LOUIS,MO. DEPT VET AFFAIRS MED CTR,WASHINGTON,DC. UNIV ARKANSAS MED SCI HOSP,LITTLE ROCK,AR 72205. UNIV FLORIDA,GAINESVILLE,FL. UNIV MINNESOTA,MINNEAPOLIS,MN. RP Klein, S (reprint author), WASHINGTON UNIV,SCH MED,660 S EUCLID AVE,BOX 8127,ST LOUIS,MO 63110, USA. NR 256 TC 224 Z9 245 U1 2 U2 5 PU AMER SOC PARENTERAL & ENTERAL NUTRITION PI SILVER SPRING PA 8630 FENTON STREET SUITE 412, SILVER SPRING, MD 20910 SN 0148-6071 J9 JPEN-PARENTER ENTER JI J. Parenter. Enter. Nutr. PD MAY-JUN PY 1997 VL 21 IS 3 BP 133 EP 156 DI 10.1177/0148607197021003133 PG 24 WC Nutrition & Dietetics SC Nutrition & Dietetics GA XA043 UT WOS:A1997XA04300002 PM 9168367 ER PT J AU Heimburger, DC Geels, WJ Bilbrey, J Redden, DT Keeney, C AF Heimburger, DC Geels, WJ Bilbrey, J Redden, DT Keeney, C TI Effects of small-peptide and whole-protein enteral feedings on serum proteins and diarrhea in critically ill patients: A randomized trial SO JOURNAL OF PARENTERAL AND ENTERAL NUTRITION LA English DT Article ID PLASMA FIBRONECTIN; CLINICAL-TRIAL; HYPOALBUMINEMIC PATIENTS; NUTRITION; DIET; FORMULA; ABSORPTION; STARVATION; TOLERANCE; NEED AB Background: It has been proposed that enteral feeding formulas containing small peptides are more efficacious and better tolerated than whole-protein formulas in critically ill patients. Methods: Intensive care unit patients were stratified with regard to treatment with antibiotics and serum albumin and randomized to treatment with a small-peptide enteral diet or. an isoenergetic, isonitrogenous whole-protein diet for 10 days. To assess efficacy, we measured serum prealbumin and fibronectin, and to assess tolerance, we monitored the incidence of diarrhea. A protocol was followed to ascertain all causes of diarrhea (defined as >200 g stool or greater than or equal to 3 liquid stools on 2 consecutive days). Results: Fifty subjects completed the trial. Serum prealbumin and fibronectin increased between 21% and 36% in both groups, but the increase was significant only in the small-peptide group. The change in fibronectin between days 5 and 10 was significantly greater in the small-peptide group (p = .02). Diarrhea occurred in 10 subjects (17.8% of days) receiving small-peptide feeding and 4 subjects (7.5% of days) receiving whole-protein feeding (p = .07 for incidence and 0.03 for prevalence), but the difference was explained by the coincidental use of more diarrhea-causing medications in the former Only one case of diarrhea could be attributed to tube feeding. Conclusions: During 10 days of feeding, the small-peptide diet produced slightly greater increases in serum rapid-synthesis proteins than did the whole-protein diet, especially between days 5 and 10. The clinical implications of this difference between the diets are unknown. Both small-peptide and whale-protein diets were well tolerated. C1 UNIV ALABAMA,DEPT MED,BIRMINGHAM,AL 35294. UNIV ALABAMA,DEPT NURSING,BIRMINGHAM,AL 35294. UNIV ALABAMA,BIOSTAT UNIT,CTR COMPREHENS CANC,BIRMINGHAM,AL 35294. BIRMINGHAM VET AFFAIRS MED CTR,DEPT DIETET,BIRMINGHAM,AL. UNIV ALABAMA HOSP & CLIN,DEPT FOOD & NUTR SERV,BIRMINGHAM,AL. RP Heimburger, DC (reprint author), UNIV ALABAMA,DEPT NUTR SCI,UAB STN,BIRMINGHAM,AL 35294, USA. FU NCI NIH HHS [CA-47888, CA-28103] NR 30 TC 17 Z9 17 U1 0 U2 5 PU AMER SOC PARENTERAL & ENTERAL NUTRITION PI SILVER SPRING PA 8630 FENTON STREET SUITE 412, SILVER SPRING, MD 20910 SN 0148-6071 J9 JPEN-PARENTER ENTER JI J. Parenter. Enter. Nutr. PD MAY-JUN PY 1997 VL 21 IS 3 BP 162 EP 167 DI 10.1177/0148607197021003162 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA XA043 UT WOS:A1997XA04300004 PM 9168369 ER PT J AU Schlesinger, N Baker, DG Schumacher, HR AF Schlesinger, N Baker, DG Schumacher, HR TI Persian Gulf War myalgia syndrome SO JOURNAL OF RHEUMATOLOGY LA English DT Letter C1 UNIV PENN,SCH MED,VET AFFAIRS MED CTR,PHILADELPHIA,PA 19104. RP Schlesinger, N (reprint author), ALLEGHENY UNIV HOSP,PHILADELPHIA,PA, USA. NR 6 TC 3 Z9 3 U1 0 U2 0 PU J RHEUMATOL PUBL CO PI TORONTO PA 920 YONGE ST, SUITE 115, TORONTO ON M4W 3C7, CANADA SN 0315-162X J9 J RHEUMATOL JI J. Rheumatol. PD MAY PY 1997 VL 24 IS 5 BP 1018 EP 1019 PG 2 WC Rheumatology SC Rheumatology GA WY021 UT WOS:A1997WY02100047 PM 9150108 ER PT J AU Cherrier, MM Mendez, MF Perryman, KM Pachana, NA Miller, BL Cummings, JL AF Cherrier, MM Mendez, MF Perryman, KM Pachana, NA Miller, BL Cummings, JL TI Frontotemporal dementia versus vascular dementia: Differential features on mental status examination SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Article; Proceedings Paper CT Annual Meeting of the American-Geriatrics-Society CY MAY 01-05, 1996 CL CHICAGO, IL SP Amer Geriatr Soc ID FRONTAL-LOBE DEGENERATION; ALZHEIMERS-DISEASE CERAD; COGNITIVE STATUS EXAMINATION; PICKS-DISEASE; NEUROPSYCHOLOGICAL FINDINGS; MULTIINFARCT DEMENTIA; PRESENILE-DEMENTIA; CONSORTIUM; ESTABLISH; REGISTRY AB OBJECTIVE: After Alzheimer's disease, vascular dementia (VaD) and frontotemporal dementia (FTD) are among the most common dementing illnesses. FTD may have a neuropsychological profile similar to that of VaD, and patients with these dementias may be difficult to distinguish on clinical examination. The purpose of this study was to elucidate distinct cognitive profiles of a large group of FTD and VaD patients on a brief, clinical mental status examination. DESIGN: A comparison of 39 FTD patients and 39 VaD patients on a brief, clinical mental status examination. SETTING: A Dementia Research Center and affiliated, university hospitals. METHODS: The FTD patients were diagnosed by noncognitive clinical and neuroimaging criteria, and the VaD patients met NINDS-AIREN criteria for vascular dementia. The two dementia groups were comparable on three dementia assessment scales. MEASUREMENTS: The mental status measures included the neuropsychological battery from the Consortium to Establish a Registry for Alzheimer's Disease (CERAD), plus supplementation from the Neurobehavioral Cognitive Status Examination (NCSE) for cognitive areas not assessed by the CERAD. RESULTS: The FTD and VaD groups differed significantly on the mental status examination measures. FTD patients performed significantly better than the VaD patients on digit span and constructions, despite comparable performance by both groups on calculations. Although not statistically significant, the FTD group performed worse than the VaD group on verbal fluency and abstractions. These differences were not explained by group differences in age and education. CONCLUSION: These results suggest that cognitive differences between FTD and VaD groups reflect greater frontal pathology in contrast to relative sparing of posterior cortex and subcortical white matter in FTD. These cognitive differences as measured by a mental status examination may help distinguish between these two dementia syndromes. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROL,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,SCH MED,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024. RI Pachana, Nancy/C-6807-2016 OI Pachana, Nancy/0000-0002-8927-4205 NR 51 TC 18 Z9 19 U1 1 U2 4 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD MAY PY 1997 VL 45 IS 5 BP 579 EP 583 PG 5 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA WZ009 UT WOS:A1997WZ00900006 PM 9158578 ER PT J AU ElSaden, SM Grant, EG Sayre, J Vinuela, F Duckwiler, G AF ElSaden, SM Grant, EG Sayre, J Vinuela, F Duckwiler, G TI Transcranial color Doppler imaging of brain arteriovenous malformations in adults SO JOURNAL OF ULTRASOUND IN MEDICINE LA English DT Article DE arteries, abnormalities; arteriovenous malformations, cerebral; blood, flow dynamics; brain, abnormalities; ultrasonography, Doppler studies ID ULTRASOUND; SONOGRAPHY; DIAGNOSIS; DISORDERS; STENOSIS; ARTERY; FLOW; US AB This study was performed to investigate the utility of transcranial color Doppler sonography in evaluating adult cerebral arteriovenous malformations. Twelve patients with such malformations underwent sonography. Spectral Doppler sonographic assessment included peak systolic velocity, end diastolic velocity, and resistive index within the intracranial and extracranial vessels. Color Doppler sonography was used to visualize the site of shunting. Paired and unpaired Student's t-tests were used to determine significance of spectral findings. The nidus was seen in eight of 10 pial malformations. The nidi in two patients were located in the parietal region and not visualized. When compared with the corresponding contralateral artery, feeding arteries (identified in all 10 of the pial lesions) demonstrated diminished resistive indices (P = 0.09). Similarly, the end diastolic velocity was increased in all feeding arteries (P = 0.03). When compared with published normal values, the peak systolic and end diastolic velocities were significantly elevated and the resistive indices were low (P < 0.0003). Transcranial color Doppler sonography depicted the nidi of pial arteriovenous malformations in 80% of cases. Feeding arteries demonstrated abnormal peak systolic velocity, end diastolic velocity, and resistive index in all cases when compared with published values. Transcranial color Doppler sonography is not a good screening tool due to limited acoustical window, but it may be useful for noninvasive study of the physiology of arteriovenous malformations as they are treated. C1 UNIV CALIF LOS ANGELES,MED CTR,DEPT RADIOL,LOS ANGELES,CA 90024. RP ElSaden, SM (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,DEPT RADIOL,BLDG 500,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 14 TC 8 Z9 8 U1 0 U2 0 PU AMER INST ULTRASOUND MEDICINE PI LAUREL PA SUBSCRIPTION DEPT, 14750 SWEITZER LANE, STE 100, LAUREL, MD 20707-5906 SN 0278-4297 J9 J ULTRAS MED JI J. Ultrasound Med. PD MAY PY 1997 VL 16 IS 5 BP 327 EP 334 PG 8 WC Acoustics; Radiology, Nuclear Medicine & Medical Imaging SC Acoustics; Radiology, Nuclear Medicine & Medical Imaging GA XT749 UT WOS:A1997XT74900003 PM 9315170 ER PT J AU Olsen, JG Salih, MA Harrison, JL Herrera, I Luther, MF Kalu, DN Lifschitz, MD Katz, MS Yeh, CK AF Olsen, JG Salih, MA Harrison, JL Herrera, I Luther, MF Kalu, DN Lifschitz, MD Katz, MS Yeh, CK TI Modulation by food restriction of intracellular calcium signaling in parotid acinar cells of aging Fischer 344 rats SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES LA English DT Article ID AGED RATS; RECEPTOR STIMULATION; SALIVARY SECRETION; GLAND MEMBRANES; TRANSDUCTION; ACTIVATION; MECHANISMS; CA-2+; RESPONSIVENESS; G(ALPHA-Q/11) AB Previous studies suggest that alpha(1)-adrenergic (alpha(1)-AR)-induced intracellular calcium ([Ca2+](i)) mobilization in rat parotid acinar cells declines with age. In this study, we examined the effects of food restriction on alpha(1)-AR- and muscarinic-stimulated [Ca2+](i) mobilization in parotid acinar cells during aging. [Ca2+](i) levels in response to the (alpha(1)-AR agonist epinephrine and the muscarinic agonist carbachol were evaluated in Fura-2-loaded parotid acinar cells from ad libitum-fed (AL) and food-restricted (FR) Fisher 344 male rats at 4, 6, 14, and 24 months of age. [Ca2+](i) responses to epinephrine and carbachol 110 mu M) were significantly reduced (48% and 35%, respectively; p < .05) in cells from 24-month-old AL rats as compared to younger AL rats. In contrast, no significant reduction of epinephrine and carbachol responses was observed in 24-month-old FR animals. An age-related increase in basal [Ca2+](i) (peak around 14 months; p < .02) was observed in both AL and FR rats. In addition, basal [Ca2+](i) was higher in FR than in AL rats at 14 and 24 months of age (p < .02). These studies suggest that FR partially attenuates or delays age-related impairments in alpha(1)-AR- and muscarinic-cholinergic signal transduction systems of parotid acinar cells. Basal [Ca2+](i) also appears to be altered during aging and by FR. C1 S TEXAS VET HLTH CARE SYST, AUDIE L MURPHY DIV, GRECC, SAN ANTONIO, TX 78284 USA. S TEXAS VET HLTH CARE SYST, RES SERV, SAN ANTONIO, TX 78284 USA. UNIV TEXAS, HLTH SCI CTR, DEPT MED, SAN ANTONIO, TX 78284 USA. UNIV TEXAS, HLTH SCI CTR, DEPT PHYSIOL, SAN ANTONIO, TX 78284 USA. UNIV TEXAS, HLTH SCI CTR, DEPT DENT DIAGNOST SERV, SAN ANTONIO, TX USA. RI Salih, Mustafa/C-1222-2014 OI Salih, Mustafa/0000-0002-4379-2111 FU NIA NIH HHS [AG-01188] NR 39 TC 9 Z9 9 U1 0 U2 0 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1079-5006 EI 1758-535X J9 J GERONTOL A-BIOL JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci. PD MAY PY 1997 VL 52 IS 3 BP B152 EP B158 PG 7 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA WZ219 UT WOS:A1997WZ21900004 PM 9158549 ER PT J AU Vitiello, MV Wilkinson, CW Merriam, GR Moe, KE Prinz, PN Ralph, DD Colasurdo, EA Schwartz, RS AF Vitiello, MV Wilkinson, CW Merriam, GR Moe, KE Prinz, PN Ralph, DD Colasurdo, EA Schwartz, RS TI Successful 6-month endurance training does not alter insulin-like growth factor-I in healthy older men and women SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES LA English DT Article ID BODY-FAT DISTRIBUTION; SOMATOMEDIN-C; IGF-I; HORMONE; EXERCISE; YOUNG; RESPONSES; AGE; SECRETION; DISEASE AB Background. Lean body mass, strength, and endurance decline with advancing age, changes paralleled by declines in anabolic hormones, including growth hormone (GH) and insulin-like growth factor-I (IGF-I). Acute exercise has been shown to stimulate the GH/IGF-I axis, and long-term exercise increases GH. This study examined the effect of endurance training on IGF-I in healthy older men and women. Methods. Thirty-one healthy older men (66.9 +/- 1.0 yrs, mean +/- SEM) and 21 healthy older women (67.1 +/- 1.7 yrs) were randomized to either 3d/wk, 6-month endurance (ET3) or stretching/flexibility (SF3) protocols. Another group of 15 healthy older men (69.0 +/- 1.3 yrs) participated in a more intensive 5d/wk, 6-month endurance protocol (ET5). Before and after training, subjects were weight stabilized and participated in maximal exercise tolerance testing, body composition assessment, and fasting blood sampling. Results. ET3 training resulted in a significant increase (14%) in maximal aerobic power (VO(2)max), significant decreases in body weight (BW), fat mass (FM), and wais/hip ratio (WHR), and a significant increase in fat-free mass (FFM). No significant VO(2)max or body composition changes were observed in the SF3 group. For the ET5 group, a significant increase (22%) in VO(2)max and significant decreases in BW, FM, and WHR were observed. No significant changes in IGF-I were observed for any of the three groups. Pre- versus post-training IGF-I values were very stable (r = .86, p < .001) across subjects. Conclusions. Within-subject basal levels of IGF-I in healthy seniors were extremely stable between pre- and posttraining assessments. Two endurance training protocols of magnitudes sufficient to significantly increase aerobic capacity and decrease measures of body adiposity did not significantly increase basal levels of IGF-I in healthy older men and women. C1 UNIV WASHINGTON,DEPT PSYCHIAT & BEHAV SCI,SEATTLE,WA 98195. UNIV WASHINGTON,DEPT MED,SEATTLE,WA 98195. VA PUGET SOUND HLTH CARE SYST,AMER LAKE DIV,TACOMA,WA. FU NIMH NIH HHS [K02-MH1158, R01-MH45186, R01-MH53575] NR 25 TC 29 Z9 29 U1 0 U2 2 PU GERONTOLOGICAL SOCIETY AMER PI WASHINGTON PA 1275 K STREET NW SUITE 350, WASHINGTON, DC 20005-4006 SN 1079-5006 J9 J GERONTOL A-BIOL JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci. PD MAY PY 1997 VL 52 IS 3 BP M149 EP M154 PG 6 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA WZ219 UT WOS:A1997WZ21900011 PM 9158556 ER PT J AU Fried, LF Palevsky, PM AF Fried, LF Palevsky, PM TI Hyponatremia and hypernatremia SO MEDICAL CLINICS OF NORTH AMERICA LA English DT Review ID ANTIDIURETIC-HORMONE SECRETION; ANTI-DIURETIC HORMONE; INDUCED WATER-INTOXICATION; CENTRAL-NERVOUS-SYSTEM; THIAZIDE-INDUCED HYPONATREMIA; CHRONIC HEART-FAILURE; VASOPRESSIN SECRETION; INAPPROPRIATE SECRETION; RAPID CORRECTION; ARGININE VASOPRESSIN AB Hyponatremia and hypernatremia are common electrolyte disorders that are associated with increased morbidity and mortality. Both disturbances result from disorders of water homeostasis. This article reviews normal water homeostasis and the pathophysiology of hypo- and hypernatremia. A rationale for treatment recommendation is provided. C1 UNIV PITTSBURGH,SCH MED,DEPT MED,RENAL ELECTROLYTE DIV,PITTSBURGH,PA 15213. VA PITTSBURGH HLTH CARE SYST,MED SERV,RENAL SECT,PITTSBURGH,PA. NR 121 TC 87 Z9 95 U1 3 U2 6 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0025-7125 J9 MED CLIN N AM JI Med. Clin. N. Am. PD MAY PY 1997 VL 81 IS 3 BP 585 EP & DI 10.1016/S0025-7125(05)70535-6 PG 26 WC Medicine, General & Internal SC General & Internal Medicine GA XA501 UT WOS:A1997XA50100002 PM 9167647 ER PT J AU Pekary, AE Sattin, A Lloyd, RL AF Pekary, AE Sattin, A Lloyd, RL TI Electroconvulsive seizures increase levels of PS4, the TRH-enhancing peptide [prepro-TRH(160-169)], in rat brain SO NEUROENDOCRINOLOGY LA English DT Article DE seizures; thyrotropin-releasing hormone; thyrotropin-releasing hormone enhancing peptide; limbic system; swim test; stress ID THYROTROPIN-RELEASING-HORMONE; GENE-EXPRESSION; INHIBITING FACTOR; FUTURE-RESEARCH; PRECURSOR; PITUITARY; PREPRO-TRH-(160-169); HYPOTHALAMUS; SECRETION; BINDING AB We report the development of a radioimmunoassay for prepro-TRH(160-169) (PS4), a thyrotropin-releasing hormone (TRH) enhancing peptide, and its use in characterizing the effect of electroconvulsive seizures on the levels of this peptide in various brain regions of male Wistar rats. We found that electroconvulsive seizures significantly elevated the PS4 levels in hippocampus, amygdala, pyriform (olfactory) cortex, and anterior cortex but not in striatum, motor cortex, locus ceruleus, or ventral lateral medulla. The levels of PS4 were highly correlated with the corresponding TRH (p-Glu-His-Pro-NH2) and TRH-Gly (p-Glu-His-Pro-Gly) levels in hippocampus, amygdala, and pyriform cortex, consistent with the prepro-TRH source of all of these peptides. The PS4 levels in hippocampus and amygdala were significantly correlated with the immobility time in the Porsolt forced swim test, an established animal model for antidepressant effects. The PS4 levels in peripheral blood, hypothalamus, anterior cortex, amygdala, and eyes increased severalfold at 20 min following intracisternal injection of 228 mu g of this peptide, suggesting that it readily crosses the blood-brain barrier. The pituitary levels of PS4 and TRH-Gly, on the other hand, were decreased within 20 min by intracisternal PS4, suggesting PS4 stimulated the release of prepro-TRH peptides from the pituitary. Fresh rat and human serum rapidly degraded PS4, indicating that it may act primarily as a paracrine modulator of TRH effects in pituitary, brain, and reproductive system. C1 UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,PSYCHIAT SERV,LOS ANGELES,CA 90073. RP Pekary, AE (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,RES SERV,11301 WILSHIRE BLVD,BLDG 114,RM 200,LOS ANGELES,CA 90073, USA. NR 44 TC 17 Z9 17 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0028-3835 J9 NEUROENDOCRINOLOGY JI Neuroendocrinology PD MAY PY 1997 VL 65 IS 5 BP 377 EP 384 PG 8 WC Endocrinology & Metabolism; Neurosciences SC Endocrinology & Metabolism; Neurosciences & Neurology GA WY144 UT WOS:A1997WY14400009 PM 9158070 ER PT J AU Kaye, JA Swihart, T Howieson, D Dame, A Moore, MM Karnos, T Camicioli, R Ball, M Oken, B Sexton, G AF Kaye, JA Swihart, T Howieson, D Dame, A Moore, MM Karnos, T Camicioli, R Ball, M Oken, B Sexton, G TI Volume loss of the hippocampus and temporal lobe in healthy elderly persons destined to develop dementia SO NEUROLOGY LA English DT Article ID MINI-MENTAL-STATE; ALZHEIMERS-DISEASE; OLDEST-OLD; ATROPHY; POPULATION; DIAGNOSIS; AMYGDALA; MEMORY; SCALE; BRAIN AB Objective: To determine initial locus and rate of degeneration of temporal lobe structures (total lobe, hippocampus and parahippocampus) in preclinical dementia. Background: Postmortem studies suggest that the earliest changes in Alzheimer's disease are neurofibrillary tangle formation in hippocampus and adjacent cortex. MRI volume analysis of temporal lobe structures over time in subjects prior to developing dementia may allow the identification of when these processes begin, the rate they develop, and which areas are key to symptom development. Methods: 30 nondemented (NoD), healthy, elderly individuals enrolled in a prospective study of healthy aging evaluated annually over a mean of 42 months. Twelve subjects with subsequent cognitive decline were assigned to the preclinical dementia group (PreD). All 120 annual MRI studies analyzed by volumetric techniques assessed group differences in temporal lobe volumes and rates of brain loss. Results: NoD as well as PreD subjects had significant, time-dependent decreases in hippocampal and parahippocampal volume. Rates of volume loss between the groups did not significantly differ. PreD cases had significantly smaller hippocampi when asymptomatic. Parahippocampal volume did not differ between PreD and NoD cases. Significant time-dependent temporal lobe atrophy was present only in PreD. Conclusions: Hippocampal and parahippocampal atrophy occurs at a similar rate regardless of diagnostic group. Those who develop dementia may have smaller hippocampi to begin with, but become symptomatic because of accelerated loss of temporal lobe volume. Temporal lobe volume loss may mark the beginning of the disease process within six years prior to dementia onset. C1 OREGON HLTH SCI UNIV,DEPT PATHOL,PORTLAND,OR 97201. OREGON HLTH SCI UNIV,DEPT SURG & MED,PORTLAND,OR 97201. PORTLAND VET AFFAIRS MED CTR,PORTLAND,OR. RP Kaye, JA (reprint author), OREGON HLTH SCI UNIV,DEPT NEUROL,CDW2,3181 SAM JACKSON PK RD,PORTLAND,OR 97201, USA. OI Kaye, Jeffrey/0000-0002-9971-3478; Camicioli, Richard/0000-0003-2977-8660 FU NIA NIH HHS [AG08017] NR 47 TC 284 Z9 291 U1 2 U2 7 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0028-3878 J9 NEUROLOGY JI Neurology PD MAY PY 1997 VL 48 IS 5 BP 1297 EP 1304 PG 8 WC Clinical Neurology SC Neurosciences & Neurology GA WZ778 UT WOS:A1997WZ77800027 PM 9153461 ER PT J AU Cummings, JL AF Cummings, JL TI The neuropsychiatric inventory: Assessing psychopathology in dementia patients SO NEUROLOGY LA English DT Article ID PROBABLE ALZHEIMERS-DISEASE; NURSING-HOME; PSYCHIATRIC-SYMPTOMS; EXTRAPYRAMIDAL SIGNS; DELUSIONS; PSYCHOSIS; SPECT; DEPRESSION; CAREGIVERS; PREDICTORS AB The Neuropsychiatric Inventory (NPI) was developed to assess psychopathology in dementia patients. It evaluates 12 neuropsychiatric disturbances common in dementia: delusions, hallucinations, agitation, dysphoria, anxiety, apathy, irritability, euphoria, disinhibition, aberrant motor behavior, night-time behavior disturbances, and appetite and eating abnormalities. The severity and frequency of each neuropsychiatric symptom are rated on the basis of scripted questions administered to the patient's caregiver. The NPI also assesses the amount of caregiver distress engendered by each of the neuropsychiatric disorders. A total NPI score and a total caregiver distress score are calculated, in addition to the scores for the individual symptom domains. Content validity, concurrent validity, inter-rater reliability, and test-retest reliability of the NPI are established. Different neurologic disorders have characteristic neuropsychiatric manifestations and distinctive NPI profiles. The NPI is sensitive to treatment effects and has demonstrated the amelioration of behavioral symptoms in Alzheimer's disease by cholinergic agents. The NPI is a useful instrument for characterizing the psychopathology of dementia syndromes, investigating the neurobiology of brain disorders with neuropsychiatric manifestations, distinguishing among different dementia syndromes, and assessing the efficacy of treatment. C1 UNIV CALIF LOS ANGELES,SCH MED,REED NEUROL RES CTR,DEPT PSYCHIAT & BEHAV SCI,LOS ANGELES,CA 90095. W LOS ANGELES VET AFFAIRS MED CTR,PSYCHIAT SERV,NEUROBEHAV & NEUROPSYCHIAT PROGRAM,LOS ANGELES,CA 90073. RP Cummings, JL (reprint author), UNIV CALIF LOS ANGELES,SCH MED,REED NEUROL RES CTR,DEPT NEUROL,710 WESTWOOD PLAZA,LOS ANGELES,CA 90095, USA. FU NIA NIH HHS [AG10123] NR 62 TC 542 Z9 558 U1 1 U2 26 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0028-3878 J9 NEUROLOGY JI Neurology PD MAY PY 1997 VL 48 IS 5 SU 6 BP S10 EP S16 PG 7 WC Clinical Neurology SC Neurosciences & Neurology GA XA096 UT WOS:A1997XA09600003 PM 9153155 ER PT J AU Chauhan, N Siegel, G AF Chauhan, N Siegel, G TI Na,K-ATPase: Increases in alpha 1-messenger RNA and decreases in alpha 3-messenger RNA levels in aging rat cerebral cortex SO NEUROSCIENCE LA English DT Article DE in situ hybridization; gene expression; brain ID DIFFERENTIAL DISTRIBUTION; ALZHEIMERS-DISEASE; NERVOUS-SYSTEM; NA+,K+-ATPASE; ISOFORMS; BRAIN AB Age-related changes in the expression of Na,K-ATPase catalytic (alpha) subunit isoform messenger RNAs were investigated by in situ hybridization in frontal-parietal cortex of Fischer-344 male rats, It was found that the density of alpha 1-messenger RNA, present in glia and some neurons, increases dramatically in the neuropil of cortex and in the corpus callosum of the aged (24 months) as compared to young (three months) rats. In contrast, levels of alpha 3-messenger RNA, which is neuron-specific, decrease dramatically over the neurons of layers III and V and retrosplenial cortex in the aged as compared to the young animals, despite no changes in cellular density. The declines in the levels of neuron-specific alpha 3-messenger RNA may constitute a very early change in age-related differentiation of brain, These changes in Na,K-ATPase isoform expression may represent a candidate for an age-related factor predisposing to or potentiating certain neurodegenerative diseases. C1 US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,MOL & CELLULAR NEUROSCI LAB,HINES,IL 60141. LOYOLA UNIV,DEPT NEUROL,MAYWOOD,IL 60153. LOYOLA UNIV,DEPT MOL & CELLULAR BIOCHEM,MAYWOOD,IL 60153. NR 15 TC 4 Z9 4 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0306-4522 J9 NEUROSCIENCE JI Neuroscience PD MAY PY 1997 VL 78 IS 1 BP 7 EP 11 PG 5 WC Neurosciences SC Neurosciences & Neurology GA WR750 UT WOS:A1997WR75000002 PM 9135086 ER PT J AU Anderson, CA Wilkening, GN Filley, CM Reardon, MS Kleinschmidt-DeMasters, BK AF Anderson, CA Wilkening, GN Filley, CM Reardon, MS Kleinschmidt-DeMasters, BK TI Neurobehavioral outcome in pediatric craniopharyngioma SO PEDIATRIC NEUROSURGERY LA English DT Article DE neurobehavior; craniopharyngioma, pediatric; outcome, neurobehavioral ID CHILDHOOD CRANIOPHARYNGIOMA; SURGICAL-MANAGEMENT; RADIATION-THERAPY; CHILDREN; SURGERY; RADIOTHERAPY; RECURRENCE; SURVIVORS; SYMPTOMS; REMOVAL AB Neurobehavioral dysfunction occurs in children with craniopharyngioma, both before and after treatment, and its impact on outcome may not be fully appreciated. Also unclear is whether neurobehavioral outcome relates more to tumor location or surgical factors. We reviewed the records of 20 children with craniopharyngioma who were seen between 1983 and 1995. All children had subfrontal craniotomy and either partial (14 children) or gross total (6 children) resection of their tumors. In addition to traditional neuropsychological testing, we assessed social behavior and school performance using standardized ratings based on family interviews and school records. Over a mean follow-up period of 38 months, only 3 of 20 children had a good outcome in all three categories, and 12 of 20 had moderate or severe impairment in at least one category. Outcome did not differ between those who had partial and those with gross total resection. We conclude that neurobehavioral disorders are common and cause important morbidity in children after treatment for craniopharyngioma. To evaluate these impairments in future outcome studies, standard neuropsychological testing should be supplemented by specific behavioral assessments to capture the full range of neurobehavioral disability. In this series, partial versus gross total resection did not influence outcome, implying that tumor location in diencephalic and limbic regions is a more important factor. C1 Univ Colorado, Hlth Sci Ctr, Dept Neurol, Denver, CO 80262 USA. Univ Colorado, Hlth Sci Ctr, Dept Pediat, Denver, CO 80262 USA. Univ Colorado, Hlth Sci Ctr, Dept Psychiat, Denver, CO 80262 USA. Univ Colorado, Hlth Sci Ctr, Dept Pathol, Denver, CO 80262 USA. Univ Colorado, Hlth Sci Ctr, Sch Med, Denver, CO 80262 USA. Childrens Hosp, Denver, CO 80218 USA. Denver Vet Affairs Med Ctr, Denver, CO USA. RP Anderson, CA (reprint author), Univ Colorado, Hlth Sci Ctr, Dept Neurol, 4200 E 9th Ave, Denver, CO 80262 USA. NR 50 TC 39 Z9 39 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 1016-2291 J9 PEDIATR NEUROSURG JI Pediatr. Neurosurg. PD MAY PY 1997 VL 26 IS 5 BP 255 EP 260 PG 6 WC Clinical Neurology; Pediatrics; Surgery SC Neurosciences & Neurology; Pediatrics; Surgery GA YP821 UT WOS:000071318500004 PM 9440495 ER PT J AU Neuhauser, TS Lancaster, K Haws, R Drehner, D Gulley, ML Lichy, JH Taubenberger, JK AF Neuhauser, TS Lancaster, K Haws, R Drehner, D Gulley, ML Lichy, JH Taubenberger, JK TI Rapidly progressive T cell lymphoma presenting as acute renal failure: Case report and review of the literature SO PEDIATRIC PATHOLOGY & LABORATORY MEDICINE LA English DT Article DE lymphoma; renal failure; T cell ID NON-HODGKINS-LYMPHOMA; MALIGNANT-LYMPHOMA; KIDNEY; INVOLVEMENT; CHILDREN; TUMOR AB We describe a case of peripheral T cell lymphoma that is remarkable fro its fulminate course and selective targeting of both kidneys. The patient was a 6-year-old girl who was in her usual state of good health until the onset of abdominal pain and fever. She was treated for acute oliguric renal failure and visual disturbances. A renal biopsy was performed. Biopsy findings were interpreted as suggestive of a vasculitic process, and treatment was initiated for a presumptive diagnosis of Wegener's granulomatosis. The patient died 3 days following admission, and autopsy revealed extensive bilateral kidney infiltration by a peripheral T cell lymphoma. The remainder of the body was spared with the exception of mild infiltration of the pulmonary parenchyma and choroid plexus by neoplastic lymphocytes. The neoplastic nature of the disease was confirmed utilizing immunoperoxidase stains and T cell receptor gene rearrangement. Primary renal lymphoma and renal failure attributable to involvement by lymphoma are rare findings that should be considered when other more common causes of renal insufficiency have been excluded. The presenting clinical complaints are generally of short duration, nonspecific, and atypical. Most patients exhibit oliguria. Physical examination may reveal hepatosplenomegaly, lymphadenopathy, and flank and/or abdominal mass(es). Laboratory findings frequently include an elevated serum creatinine, blood urea nitrogen, lactate dehydrogenase, and a mild proteinuria. Electrolyte abnormalities are variably present. Possible radiographic findings include hypodense or hypoechoic renal lesions and diffuse bilateral renal enlargement. Although the prognosis is dismal, survival may be prolonged utilizing current treatment modalities, and rare patients may be ''cured'' of disease. The clinical presentation, radiological findings, and prognosis of patients with clinically evident renal involvement by non-Hodgkin's lymphoma are discussed. C1 WILFORD HALL USAF MED CTR,DEPT PATHOL,SAN ANTONIO,TX 78236. UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. ARMED FORCES INST PATHOL,MOL DIAGNOST LAB,WASHINGTON,DC 20306. RP Neuhauser, TS (reprint author), WILFORD HALL USAF MED CTR,DEPT PATHOL,13754 MORNINGBLUFF DR,SAN ANTONIO,TX 78216, USA. NR 47 TC 16 Z9 16 U1 0 U2 3 PU TAYLOR & FRANCIS PI BRISTOL PA 1900 FROST ROAD, SUITE 101, BRISTOL, PA 19007-1598 SN 1077-1042 J9 PEDIATR PATHOL LAB M JI Pediatr. Pathol. Lab. Med. PD MAY-JUN PY 1997 VL 17 IS 3 BP 449 EP 460 DI 10.1080/107710497174741 PG 12 WC Pathology; Pediatrics SC Pathology; Pediatrics GA XC161 UT WOS:A1997XC16100007 PM 9185223 ER PT J AU Keyes, LE Majack, R Dempsey, EC Moore, LG AF Keyes, LE Majack, R Dempsey, EC Moore, LG TI Pregnancy stimulation of DNA synthesis and uterine blood flow in the guinea pig SO PEDIATRIC RESEARCH LA English DT Article ID ESTROUS-CYCLE; ARTERIES AB Pregnancy stimulates DNA synthesis in uterine artery smooth muscle cells. Unknown is whether DNA synthesis increases in all layers of the vessel wall in uterine or nonuterine vessels, the distribution and time course of the proliferative response in relation to the rise in uterine blood flow, and the extent to which a pregnancy-induced rise in DNA synthesis can be mimicked by chronic estradiol treatment. To measure DNA synthesis, we implanted bromodeoxyuridine (BrdU, 400 mg) s.c. for 14-d periods in three nonpregnant, nine pregnant, three vehicle, and five estradiol (2.5 mg/14 d)-treated guinea pigs. Uterine blood flow was measured in four nonpregnant and 18 pregnant animals using radiolabeled microspheres. Pregnancy stimulated DNA synthesis in the adventitia, media, and intima of the uterine artery, radial artery (the vessels deriving from the main uterine artery and entering the uterine wall), and uterine vein but not in the aorta or mesenteric artery. Maximal uterine artery medial area and labeling indices in all layers of the uterine artery, uterine vein, and the radial artery adventitia were attained by mid-pregnancy (d 28-42 of the guinea pig's 63-day gestation), whereas DNA synthesis increased progressively until term in the radial artery media and intima. The greatest rise in uterine artery blood flow (y) occurred after peak proliferation in the uterine artery and in concert with radial artery medial and intimal proliferation (y = 1.99.10(0.023x) where x is day postconception). (17)beta-Estradiol treatment for 14 d in ovariectomized guinea pigs increased DNA synthesis in the radial artery adventitia and tended (p = 0.08) to increase labeling indices in the media of all vessels examined but did not fully reproduce the effects of pregnancy. We concluded that pregnancy-related, possibly hormonal stimuli prompted growth in all layers of the uterine artery wall by mid-pregnancy and served to initiate a rise in uterine blood flow. The resultant increase in flow and shear stress likely stimulated DNA synthesis in the radial artery which helped sustain the rise in flow near term. C1 UNIV COLORADO,HLTH SCI CTR,CVP RES LAB,DENVER,CO 80262. YALE UNIV,SCH MED,NEW HAVEN,CT 06510. UNIV COLORADO,HLTH SCI CTR,WOMENS HLTH RES CTR,DENVER,CO 80262. UNIV COLORADO,HLTH SCI CTR,DEPT PEDIAT,DENVER,CO 80262. DENVER VA MED CTR,RESP CARE SECT,DENVER,CO 80220. UNIV COLORADO,DEPT ANTHROPOL,DENVER,CO 80217. FU NHLBI NIH HHS [HL 14985] NR 24 TC 21 Z9 21 U1 0 U2 2 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD MAY PY 1997 VL 41 IS 5 BP 708 EP 715 DI 10.1203/00006450-199705000-00017 PG 8 WC Pediatrics SC Pediatrics GA WV663 UT WOS:A1997WV66300017 PM 9128295 ER PT J AU Sullivan, G Young, AS Morgenstern, H AF Sullivan, G Young, AS Morgenstern, H TI Behaviors as risk factors for rehospitalization: Implications for predicting and preventing admissions among the seriously mentally ill SO SOCIAL PSYCHIATRY AND PSYCHIATRIC EPIDEMIOLOGY LA English DT Article ID SCHIZOPHRENIA; RELAPSE; SYSTEM AB This case-control study investigated the extent to which aberrant behaviors, in contrast to more traditional clinical factors (such as symptoms and alcohol abuse), place individuals with schizophrenia at increased risk for rehospitalization. One hundred and one recidivists (cases) were matched to 101 non-recidivists (controls) on gender, race, and time since index hospitalization. Key informants, usually family members, were interviewed to assess behaviors during a 2-week period. After controlling for possible confounding variables, we found that each aberrant behavior increased the risk for rehospitalization, but highly disruptive or dangerous behaviors (such as threatening others, acting very bizarrely, or attempting suicide) conveyed a markedly high degree of risk (adjusted odds ratio = 83.9). It is possible that service providers may be able to avert the fiscal and emotional cost of hospitalization by collaborating more closely with family members to identify these behaviors and intervene before hospitalization becomes unavoidable. C1 UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,DEPT PSYCHIAT,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,NEUROPSYCHIAT HOSP,W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH PUBL HLTH,DEPT EPIDEMIOL,LOS ANGELES,CA 90024. RP Sullivan, G (reprint author), RAND CORP,1700 MAIN ST,POB 2138,SANTA MONICA,CA 90407, USA. RI Young, Alexander/A-1523-2009 OI Young, Alexander/0000-0002-9367-9213 FU NIMH NIH HHS [MH47907, MH49199] NR 27 TC 8 Z9 8 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0933-7954 J9 SOC PSYCH PSYCH EPID JI Soc. Psychiatry Psychiatr. Epidemiol. PD MAY PY 1997 VL 32 IS 4 BP 185 EP 190 DI 10.1007/BF00788237 PG 6 WC Psychiatry SC Psychiatry GA WZ122 UT WOS:A1997WZ12200002 PM 9184463 ER PT J AU Qureshi, N Kaltashov, I Walker, K Doroshenko, V Cotter, RJ Takayama, K Sievert, TR Rice, PA Lin, JSL Golenbock, DT AF Qureshi, N Kaltashov, I Walker, K Doroshenko, V Cotter, RJ Takayama, K Sievert, TR Rice, PA Lin, JSL Golenbock, DT TI Structure of the monophosphoryl lipid a moiety obtained from the lipopolysaccharide of Chlamydia trachomatis SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID PERFORMANCE LIQUID-CHROMATOGRAPHY; RHODOPSEUDOMONAS-SPHAEROIDES; SALMONELLA-TYPHIMURIUM; NEISSERIA-GONORRHOEAE; MASS-SPECTROMETRY; FATTY-ACIDS; INFECTION; PURIFICATION; DESORPTION; MUTANT AB Monophosphoryl lipid A was prepared from the lipopolysaccharide of Chlamydia trachomatis, converted to the methyl ester, and fractionated by reverse-phase high-performance liquid chromatography. The peak fractions were collected and analyzed by mass spectrometry. Matrix-assisted laser desorption/ionization and liquid secondary ion mass spectrometry of the first of two major high-performance liquid chromatographic fractions showed multiple quasi-molecular ions of MNa+ at m/z 1780, 1794, 1808, 1822, and 1836. The positive-ion Liquid secondary ion mass spectrometry spectrum also showed a minor series of peaks at mit 1916, 1930, 1944, 1958, and 1971, consistent with the formation of matrix adducts with 3-nitrobenzyl alcohol. Oxonium ions representing the distal subunit were observed at mit 1057, 1071, 1085, 1099, and 1113. The second fraction was similarly analyzed and found to contain structural homologs of the first fraction. Based on this study, the major lipid A component of chlamydial lipopolysaccharide is a glucosamine disaccharide that contains five fatty acids and a phosphate in the distal segment. Three fatty acyl groups are in the distal segment, and two are in the reducing end segment. The acyloxyacyl group is located in the distal segment in amide linkage. Two structural series, differing by 14 atomic mass units in the reducing subunit, were observed. Chlamydial lipid A is complex and consists of at least 20 homologous structural components. The relatively low potency of Chlamydia trachomatis lipopolysaccharide in activating lipopolysaccharide-responsive cells might be related to the unusual fatty acid composition of the lipid A moiety. C1 UNIV WISCONSIN,DEPT BACTERIOL,MADISON,WI 53706. JOHNS HOPKINS UNIV,SCH MED,DEPT PHARMACOL & MOL SCI,BALTIMORE,MD 21205. BOSTON MED CTR,MAXWELL FINLAND LAB INFECT DIS,BOSTON,MA 02118. BOSTON UNIV,SCH MED,BOSTON,MA 02118. RP Qureshi, N (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,MYCOBACTERIOL RES LAB,2500 OVERLOOK TERRACE,RM D-2215,MADISON,WI 53705, USA. FU NIAID NIH HHS [AI-33087, AI-38515]; NIGMS NIH HHS [GM-50870] NR 22 TC 52 Z9 52 U1 0 U2 5 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD APR 18 PY 1997 VL 272 IS 16 BP 10594 EP 10600 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA WV262 UT WOS:A1997WV26200040 PM 9099706 ER PT J AU Fihn, SD McDonell, MB Henikoff, J AF Fihn, SD McDonell, MB Henikoff, J TI Warfarin-related bleeding in the elderly - Response SO ANNALS OF INTERNAL MEDICINE LA English DT Letter ID RANDOMIZED TRIAL; FOLLOW-UP; ANTICOAGULATION; MULTICENTER RP Fihn, SD (reprint author), VET AFFAIRS PUGET SOUND HLTH CARE SYST,SEATTLE,WA 98108, USA. NR 5 TC 1 Z9 1 U1 0 U2 0 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD APR 15 PY 1997 VL 126 IS 8 BP 660 EP 661 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA WT580 UT WOS:A1997WT58000017 ER PT J AU Cardenas, VA Gill, P Fein, G AF Cardenas, VA Gill, P Fein, G TI Human P50 suppression is not affected by variations in wakeful alertness SO BIOLOGICAL PSYCHIATRY LA English DT Article DE singular value decomposition; C-T ratio; event-related potentials; beta power; auditory P50 ID AUDITORY-EVOKED-RESPONSES; SCHIZOPHRENIA; RELIABILITY; SLEEP; NORMALIZATION; POTENTIALS; WAVE; CAT AB The amplitude and suppression of the auditory P50 event-related potential may be useful for studying schizophrenia and drug abuse; however, the low reliability of the P50 suppression measure limits its value for correlation with clinical measures. Reliability can be increased either by improving measurement methods or by reducing or eliminating sources of variance in the recordings. In this paper the effect on P50 amplitude and suppression of variation in wakeful alertness within an experimental session was examined in 20 normal subjects. The percentage of beta power in the interval immediately prior to the P50 stimuli was used as an index of alertness. P50 amplitudes or C-T ratios were estimated using peak-picking and using the singular value decomposition (SVD) method. No effects of variation of wakeful alertness were observed on arty P50 amplitude or suppression measure. Comparing the peak-picking vs SVD estimates replicated our prior results showing markedly higher reliabilities with SVD. We conclude: 1) that variation within an experimental session in wakeful alertness level as indexed by the percentage of beta power does not affect P50 amplitude or suppression, and 2) the SVD method brings the reliability of the C-T ratio up to levels where its usefulness in clinical studies can be examined. (C) 1997 Society of Biological Psychiatry. C1 UNIV CALIF SAN FRANCISCO,DEPT PSYCHIAT,SAN FRANCISCO,CA 94143. SAN FRANCISCO VET ADM MED CTR,PSYCHIAT SERV,SAN FRANCISCO,CA. FU NIDA NIH HHS [R01DA08365] NR 41 TC 20 Z9 20 U1 1 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 15 PY 1997 VL 41 IS 8 BP 891 EP 901 DI 10.1016/S0006-3223(96)00186-2 PG 11 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA WT661 UT WOS:A1997WT66100008 PM 9099416 ER PT J AU Cohen, RD Castellani, LW Qiao, JH VanLenten, BJ Lusis, AJ Reue, K AF Cohen, RD Castellani, LW Qiao, JH VanLenten, BJ Lusis, AJ Reue, K TI Reduced aortic lesions and elevated high density lipoprotein levels in transgenic mice overexpressing mouse apolipoprotein A-IV SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE apo A-IV; atherosclerosis; lipoproteins; cellular cholesterol efflux; transgene ID LECITHIN-CHOLESTEROL ACYLTRANSFERASE; GENETICALLY-ENGINEERED MICE; HUMAN-PLASMA; APOA-I; INTESTINAL EXPRESSION; INTERSTITIAL FLUID; MESENTERIC LYMPH; TANDEM REPEATS; MESSENGER-RNA; III GENE AB Transgenic mouse lines carrying several copies of the mouse apo A-IV gene were produced. Lipoprotein composition and function, and aortic lesion development were examined. Apo A-IV levels in the plasma of transgenic mice were elevated threefold compared with nontransgenic littermates on a chow diet, and sixfold in mice fed an atherogenic diet. Plasma concentrations of total cholesterol, HDL cholesterol, triglycerides, and free fatty acids were similar in transgenic and control mice fed a chow diet. However, with the atherogenic diet, male transgenic mice exhibited significantly higher levels of plasma triglycerides (P < 0.05), total cholesterol (P < 0.01), HDL cholesterol (P < 0.0001), and free fatty acids (P < 0.05), and lower levels of unesterified cholesterol (P < 0.05), than nontransgenic littermates. Expression of the apo A-IV transgene had a protective effect against the formation of diet-induced aortic lesions, with transgenics exhibiting lesion scores of similar to 30% those seen in control mice. HDL-sized lipoproteins isolated from transgenic mice fed the atherogenic diet promoted cholesterol efflux from cholesterol-loaded human monocytes more efficiently than comparable lipoproteins from nontransgenic counterparts. Plasma from transgenics also exhibited higher endogenous cholesterol esterification rates. Taken together, these results suggest that apo A-IV levels influence the metabolism and antiatherogenic properties of HDL. C1 W LOS ANGELES VET AFFAIRS MED CTR,LIPID RES LAB,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90095. UNIV CALIF LOS ANGELES,DEPT MICROBIOL & MOL GENET,LOS ANGELES,CA 90095. UNIV CALIF LOS ANGELES,INST MOL BIOL,LOS ANGELES,CA 90095. FU NHLBI NIH HHS [HL-30568, HL-28481] NR 59 TC 119 Z9 121 U1 0 U2 1 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD APR 15 PY 1997 VL 99 IS 8 BP 1906 EP 1916 DI 10.1172/JCI119358 PG 11 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA WV360 UT WOS:A1997WV36000014 PM 9109435 ER PT J AU Homanics, GE DeLorey, TM Firestone, LL Quinlan, JJ Handforth, A Harrison, NL Krasowski, MD Rick, CEM Korpi, ER Makela, R Brilliant, MH Hagiwara, N Ferguson, C Snyder, K Olsen, RW AF Homanics, GE DeLorey, TM Firestone, LL Quinlan, JJ Handforth, A Harrison, NL Krasowski, MD Rick, CEM Korpi, ER Makela, R Brilliant, MH Hagiwara, N Ferguson, C Snyder, K Olsen, RW TI Mice devoid of gamma-aminobutyrate type A receptor beta 3 subunit have epilepsy, cleft palate, and hypersensitive behavior SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE gene targeting; benzodiazepine; Angelman syndrome; anesthesia ID A RECEPTOR; ACID RECEPTOR; GABA(A) RECEPTOR; MESSENGER-RNAS; ANGELMAN SYNDROME; GROWTH-FACTORS; RETINOIC ACID; RAT-BRAIN; P-LOCUS; MOUSE AB gamma-Aminobutyric acid type A receptors (GABA(A)-Rs) mediate the bulk of rapid inhibitory synaptic transmission in the central nervous system. The beta 3 subunit is an essential component of the GABA(A)-R in many brain regions, especially during development, and is implicated in several pathophysiologic processes. We examined mice harboring a beta 3 gene inactivated by gene targeting. GABA(A)-R density is approximately halved in brain of beta 3-deficient mice, and GABA(A)-R function is severely impaired. Most beta-deficient mice die as neonates; some neonatal mortality, but not all, is accompanied by cleft palate. beta 3-deficient mice that survive are runted until weaning but achieve normal body size by adulthood, although with reduced life span. These mice are fertile but mothers fail to nurture offspring. Brain morphology is grossly normal, but a number of behaviors are abnormal, consistent with the widespread location of the beta 3 subunit. The mice are very hyperactive and hyperresponsive to human contact and other sensory stimuli, and often run continuously in tight circles. When held by the tail, they hold all paws in like a ball, which is frequently a sign of neurological impairment. They have difficulty swimming, walking on grids, and fall off platforms and rotarods, although they do not have a jerky gait. beta 3-deficient mice display frequent myoclonus and occasional epileptic seizures, documented by electroencephalographic recording. Hyperactivity, lack of coordination, and seizures are consistent with reduced presynaptic inhibition in spinal cord and impaired inhibition in higher cortical centers and/or pleiotropic developmental defects. C1 UNIV CALIF LOS ANGELES, DEPT MOL & MED PHARMACOL, LOS ANGELES, CA 90095 USA. W LOS ANGELES VET AFFAIRS MED CTR, NEUROL SERV, LOS ANGELES, CA 90073 USA. UNIV CHICAGO, DEPT ANESTHESIA & CRIT CARE, CHICAGO, IL 60637 USA. UNIV TURKU, DEPT PHARMACOL & CLIN PHARMACOL, FIN-20520 TURKU, FINLAND. NATL PUBL HLTH INST, DEPT ALCOHOL RES, FIN-00101 HELSINKI, FINLAND. UNIV TAMPERE, SCH MED, FIN-33101 TAMPERE, FINLAND. FOX CHASE CANC CTR, INST CANC RES, PHILADELPHIA, PA 19111 USA. RP Homanics, GE (reprint author), UNIV PITTSBURGH, SCH MED, DEPT ANESTHESIOL CRIT CARE MED, W1356 BIOMED SCI TOWER, PITTSBURGH, PA 15261 USA. OI Homanics, Gregg/0000-0003-3641-8153 FU NIAAA NIH HHS [R01 AA010422, AA10422, R37 AA010422]; NIGMS NIH HHS [GM43840, GM52035] NR 39 TC 313 Z9 321 U1 0 U2 7 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 15 PY 1997 VL 94 IS 8 BP 4143 EP 4148 DI 10.1073/pnas.94.8.4143 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA WW810 UT WOS:A1997WW81000120 PM 9108119 ER PT J AU Mundinger, TO Boyle, MR Taborsky, GJ AF Mundinger, TO Boyle, MR Taborsky, GJ TI Activation of hepatic sympathetic nerves during hypoxic, hypotensive and glucopenic stress SO JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM LA English DT Article DE norepinephrine; epinephrine; hemorrhage; 2-deoxyglucose ID HYPERGLYCEMIC RESPONSE; LIVER; DOGS; HYPOGLYCEMIA; STIMULATION; HEMORRHAGE; METABOLISM; NOREPINEPHRINE; INNERVATION; INFUSION AB To investigate the potential for neural regulation of liver function, we sought to determine whether hepatic sympathetic nerves are activated during stress. Hepatic norepinephrine spillover (HNESO) was measured in halothane-anesthetized dogs before, during and after glucopenia, hypoxia and hemorrhage. HNESO increased during 2-deoxyglucose (2-DG, 600 mg/kg plus 13.5 mg/kg/min, IV)-induced glucopenia from a baseline of 9 +/- 3 ng/min to 83 +/- 24 ng/min (Delta = +74 +/- 23 ng/min, p < 0.01). During hypoxia (partial pressure of oxygen in arterial blood = 23 +/- 2 mmHg), HNESO increased by 142 +/- 47 ng/min (p < 0.025), and HNESO increased by 84 +/- 22 ng/min (p < 0.01) during hemorrhage (mean arterial blood pressure = 40 +/- 1 mmHg), suggesting activation of hepatic sympathetic nerves during all three stresses. To validate the use of HNESO as an index of hepatic sympathetic nerve activity, we repeated the stresses of hypoxia and hemorrhage in dogs following chemical sympathetic denervation of the liver induced by prior intraportal 6-hydroxy dopamine infusion. Hepatic denervation reduced the HNESO responses to hypoxia and hemorrhage by more than 90%. In addition to hepatic neural responses to stress, the sympathetic responses of the adrenal medulla and of systemic sympathetic nerves were monitored using changes in the arterial concentration of epinephrine and norepinephrine, respectively. Arterial epinephrine and norepinephrine increased by varying degrees during all three stresses, suggesting general sympatho-adrenal activation. As expected, 6-hydroxydopamine pretreatment did not alter the epinephrine response to hypoxia or hemorrhage. The arterial norepinephrine responses to hypoxia and hemorrhage were modestly reduced in hepatically sympathectomized animals, suggesting a small hepatic contribution to the elevated arterial level of norepinephrine during these stresses. We conclude that: (1) the stresses of glucopenia, hypoxia and hemorrhage activate the sympathetic nerves of the liver and (2) HNESO is a valid index of hepatic sympathetic nerve activity. Finally, we speculate that such activation may influence liver function. C1 VET AFFAIRS PUGET SOUND HLTH CARE SYST,SEATTLE,WA 98108. RP Mundinger, TO (reprint author), UNIV WASHINGTON,DEPT MED,DIV ENDOCRINOL & METAB 151,1660 S COLUMBIAN WAY,SEATTLE,WA 98108, USA. FU NIDDK NIH HHS [DK 17047, DK 12829] NR 28 TC 13 Z9 13 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-1838 J9 J AUTONOM NERV SYST JI J. Auton. Nerv. Syst. PD APR 14 PY 1997 VL 63 IS 3 BP 153 EP 160 DI 10.1016/S0165-1838(97)00004-0 PG 8 WC Neurosciences SC Neurosciences & Neurology GA WU783 UT WOS:A1997WU78300006 PM 9138247 ER PT J AU Every, NR Weaver, WD AF Every, NR Weaver, WD TI A comparison of thrombolytic therapy with primary coronary angioplasty for acute myocardial infarction - Reply SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Letter C1 HENRY FORD HLTH CARE SYST,DETROIT,MI 48202. RP Every, NR (reprint author), VET AFFAIRS PUGET SOUND HEALTHCARE SYST,SEATTLE,WA 98108, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD APR 10 PY 1997 VL 336 IS 15 BP 1104 EP 1104 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA WT161 UT WOS:A1997WT16100020 ER PT J AU Deutsch, JC AF Deutsch, JC TI Ascorbic acid and dehydroascorbic acid interconversion without net oxidation or reduction SO ANALYTICAL BIOCHEMISTRY LA English DT Article ID PERFORMANCE LIQUID-CHROMATOGRAPHY; HUMAN-BLOOD PLASMA; LIPID-PEROXIDATION; BIOLOGICAL SAMPLES; VITAMIN-C; ANTIOXIDANT AB The interconversion of ascorbic acid and dehydroascorbic acid was examined in aqueous solution using unlabeled dehydroascorbic acid and nonlabile, stable isotope-labeled ascorbic acid by gas chromatographic/mass spectrometric analysis. Although the formation of unlabeled ascorbic acid from unlabeled dehydroascorbic acid or labeled dehydroascorbic acid from labeled ascorbic acid did not occur to any significant extent when either solutions of unlabeled dehydroascorbic acid or labeled ascorbic acid were incubated alone, significant amounts of both labeled dehydroascorbic acid and unlabeled ascorbic acid formed when unlabeled dehydroascorbic acid was incubated with labeled ascorbic acid at acid pH. At alkaline pH, interconversion did not occur to any appreciable extent. Likewise, interconversion did not appear to occur in plasma at physiologic concentrations of ascorbic acid, but did occur with pharmacologic concentrations. These data show that ascorbic acid and dehydroascorbic acid interconvert in acidic solution, suggesting the reducing hydrogen atoms are delocalized when ascorbic acid is paired with dehydroascorbic acid under these circumstances. Alkaline pH and plasma inhibit the interconversion. (C) 1997 Academic Press. C1 UNIV COLORADO,HLTH SCI CTR,DEPT MED,DIV HEMATOL,DENVER,CO 80220. DENVER VET AFFAIRS HOSP,DENVER,CO 80220. RP Deutsch, JC (reprint author), UNIV COLORADO,HLTH SCI CTR,DEPT MED,DIV GASTROENTEROL,4200 E 9TH AVE,CAMPUS BOX B-170,DENVER,CO 80220, USA. NR 18 TC 12 Z9 12 U1 0 U2 19 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0003-2697 J9 ANAL BIOCHEM JI Anal. Biochem. PD APR 5 PY 1997 VL 247 IS 1 BP 58 EP 62 DI 10.1006/abio.1997.2035 PG 5 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA WT591 UT WOS:A1997WT59100009 PM 9126371 ER PT J AU Barzansky, B Veloski, J Nash, DB Bastacky, S Stevens, DP AF Barzansky, B Veloski, J Nash, DB Bastacky, S Stevens, DP TI Medical education and marketplace competition - Reply SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Letter C1 THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,PHILADELPHIA,PA 19107. US DEPT HHS,ROCKVILLE,MD 20852. US DEPT VET AFFAIRS,WASHINGTON,DC. RP Barzansky, B (reprint author), AMER MED ASSOC,515 N STATE ST,CHICAGO,IL 60610, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD APR 2 PY 1997 VL 277 IS 13 BP 1037 EP 1037 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA WQ089 UT WOS:A1997WQ08900023 ER PT J AU Sontag, SJ Robinson, M Roufail, W Hirschowitz, BI Sabesin, SM Wu, WC Behar, J Peterson, WL Kranz, KR Tarnawski, A Dayal, Y Berman, R Simon, TJ AF Sontag, SJ Robinson, M Roufail, W Hirschowitz, BI Sabesin, SM Wu, WC Behar, J Peterson, WL Kranz, KR Tarnawski, A Dayal, Y Berman, R Simon, TJ TI Daily omeprazole surpasses intermittent dosing in preventing relapse of oesophagitis: A US multi-centre double-blind study SO ALIMENTARY PHARMACOLOGY & THERAPEUTICS LA English DT Article ID LONG-TERM TREATMENT; REFLUX ESOPHAGITIS; RANITIDINE AB Introduction: Relapse of erosive oesophagitis occurs in almost all patients if treatment is stopped after initial healing. Aim: To assess the potential of different therapeutic regimens of omeprazole to prevent relapse of erosive reflux oesophagitis after initial healing with omeprazole, Patients and methods: Patients whose active erosive reflux oesophagitis (grade greater than or equal to 2) had healed (grade 0 or 1) after 4-8 weeks of open-label omeprazole 40 mg daily (phase I) were eligible to join a multi-centre, 6-month double-blind, placebo-controlled maintenance study (phase II), which included endoscopy, symptom assessments, serum gastrin measurements, and gastric fundic biopsies. During phase I, endoscopy was performed at weeks 0, 4, and 8. At the end of phase I, 429 of 472 patients (91%) were healed, and there were significant reductions in heartburn, dysphagia and acid regurgitation. Of the 429 patients who healed, 406 joined phase II and were randomized to one of three groups: 20 mg omeprazole daily (n = 138), 20 mg omeprazole for 3 consecutive days each week (n = 137), or placebo (n = 131). During phase II, endoscopy was performed at months 1, 3, and 6 or at symptomatic relapse. Results: The percentages of patients still in endoscopic remission at 6 months were 11% for placebo, 34% for omeprazole 3-days-a-week, and 70% for omeprazole daily, Both omeprazole regimens were superior to placebo in preventing recurrence of symptoms (P < 0.001); however, omeprazole 20 mg daily was superior to omeprazole 20 mg 3-days-a-week (P < 0.001). Compared to baseline, omeprazole therapy resulted in no significant differences among treatment groups in the distribution of gastric endocrine cells. Conclusions: These results show that after healing of erosive oesophagitis with 4-8 weeks of omeprazole, relapse of oesophagitis and recurrence of reflux symptoms can be prevented in 70% of patients with a maintenance regimen of 20 mg daily, but that intermittent dosing comprising 3 consecutive days each week significantly compromises efficacy. C1 UNIV TEXAS,SW MED CTR,DEPT MED,DALLAS,TX 75230. RP Sontag, SJ (reprint author), US DEPT VET AFFAIRS,MED SERV,POB 5000,BLDG 1 151B,HINES,IL 60141, USA. NR 12 TC 18 Z9 18 U1 0 U2 2 PU BLACKWELL SCIENCE LTD PI OXFORD PA P O BOX 88, OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0NE SN 0269-2813 J9 ALIMENT PHARM THERAP JI Aliment. Pharmacol. Ther. PD APR PY 1997 VL 11 IS 2 BP 373 EP 380 DI 10.1046/j.1365-2036.1997.141317000.x PG 8 WC Gastroenterology & Hepatology; Pharmacology & Pharmacy SC Gastroenterology & Hepatology; Pharmacology & Pharmacy GA WX244 UT WOS:A1997WX24400021 PM 9146778 ER PT J AU Kahrilas, PJ Orlando, RC Sontag, SJ Lechago, J Richter, JE Freston, JW Peura, DA Hirschowitz, BI AF Kahrilas, PJ Orlando, RC Sontag, SJ Lechago, J Richter, JE Freston, JW Peura, DA Hirschowitz, BI TI The pathogenesis of GERD: The relationship between epithelial defense, dysmotility, and acid exposure - Panel discussion SO AMERICAN JOURNAL OF GASTROENTEROLOGY LA English DT Editorial Material C1 NW MEM HOSP,DEPT GASTROENTEROL,GASTROINTESTINAL DIAGNOST LAB,CHICAGO,IL 60611. TULANE UNIV,SCH MED,DEPT PHYSIOL & MED,NEW ORLEANS,LA 70112. TULANE UNIV,MED CTR,DEPT MED,SECT GASTROENTEROL & HEPATOL,NEW ORLEANS,LA 70112. LOYOLA UNIV,STRITCH SCH MED,DEPT MED,MAYWOOD,IL 60153. US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,DEPT MED,ENDOSCOPY LAB,HINES,IL 60141. BAYLOR COLL MED,DEPT PATHOL,HOUSTON,TX 77030. METHODIST HOSP,DEPT LAB MED,HOUSTON,TX 77030. CLEVELAND CLIN FDN,DEPT GASTROENTEROL,CLEVELAND,OH 44195. UNIV CONNECTICUT,SCH MED,DEPT MED,FARMINGTON,CT. JOHN DEMPSEY HOSP,DEPT MED,FARMINGTON,CT. UNIV VIRGINIA,SCH MED,DEPT INTERNAL MED,CHARLOTTESVILLE,VA 22908. UNIV VIRGINIA,HLTH SCI CTR,DEPT INTERNAL MED,DIV GASTROENTEROL,CHARLOTTESVILLE,VA 22908. UNIV ALABAMA,SCH MED,DEPT PHYSIOL & MED,BIRMINGHAM,AL. MCMASTER UNIV,MED CTR,DIV GASTROENTEROL,HAMILTON,ON,CANADA. RP Kahrilas, PJ (reprint author), NORTHWESTERN UNIV,SCH MED,DEPT MED,CHICAGO,IL 60611, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-9270 J9 AM J GASTROENTEROL JI Am. J. Gastroenterol. PD APR PY 1997 VL 92 IS 4 SU S BP S5 EP S7 PG 3 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WW683 UT WOS:A1997WW68300003 ER PT J AU Lechago, J McGuigan, JE Freston, JW Richter, JE Sontag, SJ Parkinson, A AF Lechago, J McGuigan, JE Freston, JW Richter, JE Sontag, SJ Parkinson, A TI Long-term acid control and proton pump inhibitors: Interactions and safety issues in perspective SO AMERICAN JOURNAL OF GASTROENTEROLOGY LA English DT Editorial Material C1 METHODIST HOSP,DEPT LAB MED,HOUSTON,TX 77030. UNIV FLORIDA,SHANDS HOSP,J HILLIS MILLER HLTH CTR,COLL MED,DEPT MED,GAINESVILLE,FL. UNIV CONNECTICUT,SCH MED,DEPT MED,FARMINGTON,CT. JOHN DEMPSEY HOSP,DEPT MED,FARMINGTON,CT. CLEVELAND CLIN FDN,DEPT GASTROENTEROL,CLEVELAND,OH 44195. LOYOLA UNIV,STRITCH SCH MED,DEPT MED,MAYWOOD,IL 60153. US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,DEPT MED,ENDOSCOPY LAB,HINES,IL 60141. UNIV KANSAS,SCH MED,DEPT PHARMACOL TOXICOL & THERAPEUT,KANSAS CITY,KS. RP Lechago, J (reprint author), BAYLOR COLL MED,DEPT PATHOL,HOUSTON,TX 77030, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-9270 J9 AM J GASTROENTEROL JI Am. J. Gastroenterol. PD APR PY 1997 VL 92 IS 4 SU S BP S56 EP S57 PG 2 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WW683 UT WOS:A1997WW68300022 ER PT J AU Maton, PN Howden, CW Freston, JW Tolman, KG Hunt, RH Hirschowitz, BI Sontag, SJ Orlando, RC AF Maton, PN Howden, CW Freston, JW Tolman, KG Hunt, RH Hirschowitz, BI Sontag, SJ Orlando, RC TI Optimizing the pharmacology of acid control in acid-related disorders - Panel discussion SO AMERICAN JOURNAL OF GASTROENTEROLOGY LA English DT Editorial Material C1 UNIV S CAROLINA,SCH MED,DEPT INTERNAL MED,DIV DIGEST DIS & NUTR,COLUMBIA,SC. RICHLAND MEM HOSP,DEPT INTERNAL MED,COLUMBIA,SC. UNIV CONNECTICUT,SCH MED,DEPT MED,FARMINGTON,CT. JOHN DEMPSEY HOSP,DEPT MED,FARMINGTON,CT. UNIV UTAH,SCH MED,DEPT GASTROENTEROL INTERNAL MED,SALT LAKE CITY,UT. UNIV HOSP,DEPT GASTROENTEROL,SALT LAKE CITY,UT. MCMASTER UNIV,MED CTR,DIV GASTROENTEROL,HAMILTON,ON,CANADA. UNIV ALABAMA,SCH MED,DEPT PHYSIOL & MED,BIRMINGHAM,AL. LOYOLA UNIV,STRITCH SCH MED,DEPT MED,MAYWOOD,IL 60153. US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,DEPT MED,ENDOSCOPY LAB,HINES,IL 60141. TULANE UNIV,SCH MED,DEPT PHYSIOL & MED,NEW ORLEANS,LA 70112. TULANE UNIV,MED CTR,DEPT MED,SECT GASTROENTEROL & HEPATOL,NEW ORLEANS,LA 70112. RP Maton, PN (reprint author), PRESBYTERIAN HOSP,OKLAHOMA FDN DIGEST RES,OKLAHOMA CITY,OK 73104, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-9270 J9 AM J GASTROENTEROL JI Am. J. Gastroenterol. PD APR PY 1997 VL 92 IS 4 SU S BP S20 EP S21 PG 2 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WW683 UT WOS:A1997WW68300009 ER PT J AU Raff, H Jankowski, BM Goodfriend, TL Baker, JE Papanek, PE AF Raff, H Jankowski, BM Goodfriend, TL Baker, JE Papanek, PE TI Effect of exposure to hypoxia from birth an aldosterone in rabbits: Role of unesterified fatty acids SO AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY LA English DT Article DE adrenal cortex; zona glomerulosa cell; oleic acid; development; corticosterone ID ATRIAL-NATRIURETIC-FACTOR; ADRENOCORTICAL FUNCTION; GLOMERULOSA CELLS; ZONA GLOMERULOSA; HYPOXEMIA; RATS; INHIBITION; SECRETION; CORTICOSTERONE; MECHANISM AB Hypoxia and fluid and electrolyte disturbances are serious risks to normal postnatal development. Because a decrease in inspired O-2 (hypoxic hypoxia) inhibits aldosterone synthesis in the adult and aldosterone controls water and electrolyte balance, we studied adrenocortical function in rabbits exposed to normobaric normoxia or hypoxic hypoxia (fraction of inspired O-2 0.09) from birth. At 21 days of age, rabbits were anesthetized, the adrenals were rapidly removed, and the adrenal capsules containing mostly zona glomerulosa cells were separated. Cells were dispersed with collagenase and studied in vitro. Hypoxia in vivo resulted in a 73% decrease in basal aldosterone release and a 86% decrease in adenosine 3',5'-cyclic monophosphate-stimulated aldosterone release in vitro. We hypothesized that increased unesterified fatty acids could be partly responsible for inhibition of aldosterone synthesis. Total serum unesterified fatty acids in hypoxic kits were significantly increased (298 +/- 14 mu mol/l) compared with normoxic kits (184 +/- 31 mu mol/l). When cells from hypoxic rabbits were washed with fatty acid-free albumin and studied under conditions devoid of fatty acids, aldosterone production was partially restored. Corticosterone production was not affected by washing. Washing had no effect on aldosterone synthesis by cells from normoxic rats. Finally, exposing washed zona glomerulosa cells to oleic acid (10-50 mu M) inhibited aldosteronogenesis. We conclude that exposure to hypoxia from birth attenuates aldosterone production in part due to an increase in levels of unesterified fatty acid levels. C1 ST LUKES HOSP, ENDOCRINE RES LAB, MILWAUKEE, WI 53215 USA. MED COLL WISCONSIN, DEPT MED, MILWAUKEE, WI 53226 USA. MED COLL WISCONSIN, DEPT CARDIOTHORAC SURG, MILWAUKEE, WI 53226 USA. MARQUETTE UNIV, PROGRAM PHYS THERAPY, MILWAUKEE, WI 53201 USA. UNIV WISCONSIN, SCH MED, DEPT MED, MADISON, WI 53705 USA. UNIV WISCONSIN, SCH MED, DEPT PHARMACOL, MADISON, WI 53705 USA. WILLIAM S MIDDLETON MEM VET ADM MED CTR, MADISON, WI 53705 USA. NR 36 TC 9 Z9 9 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0363-6119 EI 1522-1490 J9 AM J PHYSIOL-REG I JI Am. J. Physiol.-Regul. Integr. Comp. Physiol. PD APR PY 1997 VL 272 IS 4 BP R1084 EP R1087 PG 4 WC Physiology SC Physiology GA WT588 UT WOS:A1997WT58800011 PM 9140005 ER PT J AU Korent, VA Conhaim, RL McGrath, AM DeAngeles, DA Harms, BA AF Korent, VA Conhaim, RL McGrath, AM DeAngeles, DA Harms, BA TI Molecular distribution of hetastarch in plasma and lung lymph of unanesthetized sheep SO AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE LA English DT Article ID AWAKE SHEEP; LIQUID; HYPOPROTEINEMIA; EXCHANGE; PRESSURE; NODES AB We used high performance size exclusion chromatography (HPSEC) to measure concentrations and molecular masses of hetastarch (Het) in plasma and lung lymph of unanesthetized sheep. Our goal was to assess the osmotic effectiveness of Het in the pulmonary circulation as judged by its exclusion from lung lymph. Sheep (n = 5) received 35 ml/kg of Hat (6%) over 90 min. At the end of the infusion, Het concentrations in plasma reached a peak value of 2.9 +/- 0.1% (mean +/- SD). Lymph concentrations reached a peak value of 1.3 +/- 0.3% at 4.5 h. Het molecular masses in plasma averaged 650 +/- 36 kD at 90 min, hut ranged from 31 to 2,942 +/- 187 kD. Masses in lung lymph averaged 373 +/- 71 kD, and ranged from TB +/- 2 to 1,693 +/- 514 kD (p less than or equal to 0.05 vs. plasma). Het contributed 6.7 +/- 1,5 mm Hg to the plasma macromolecular osmotic pressure, and 3.7 +/- 1.8 mm Hg to the lymph osmotic pressure. Despite the fact that Het has the largest molecular mass of any of the current macromolecular plasma volume expanders, we found that it filtered readily into lymph, raising the lymph osmotic pressure. These findings suggest that the rationale for the osmotic performance of such solutions may need to be reconsidered. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,DEPT SURG,MADISON,WI 53705. UNIV WISCONSIN,DEPT SURG,MADISON,WI. FU NHLBI NIH HHS [HL46236] NR 28 TC 5 Z9 5 U1 0 U2 0 PU AMER LUNG ASSOC PI NEW YORK PA 1740 BROADWAY, NEW YORK, NY 10019 SN 1073-449X J9 AM J RESP CRIT CARE JI Am. J. Respir. Crit. Care Med. PD APR PY 1997 VL 155 IS 4 BP 1302 EP 1308 PG 7 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA WR657 UT WOS:A1997WR65700020 PM 9105071 ER PT J AU Canver, CC Armstrong, VM Cooler, SD Nichols, RD AF Canver, CC Armstrong, VM Cooler, SD Nichols, RD TI Assessment of internal thoracic artery vasoreactivity in response to sublingual nitroglycerin SO ANNALS OF THORACIC SURGERY LA English DT Article ID MAMMARY ARTERY; NONINVASIVE ASSESSMENT; SURGERY; BYPASS; GRAFT AB Background. We have previously shown the feasibility of assessing internal thoracic artery (ITA) size and blood now hemodynamics before and after coronary artery bypass grafting using color-flow duplex ultrasound. This noninvasive method would be an ideal diagnostic tool for the evaluation of ITA graft status after therapeutic interventions in a patient with angina after coronary artery bypass grafting. The purpose of this study was to investigate the effects of nitroglycerin on the diameter and blood flow velocities of the left native ITA before coronary artery bypass grafting and the ITA graft postoperatively. Methods. The study consisted of 24 male patients (mean age, 59 +/- 2.3 years) who had undergone elective coronary artery bypass grafting using a left ITA graft to the left anterior descending artery, with additional saphenous vein grafts. Color-flow duplex ultrasound (5.0-MHz transducer) was used for both the preoperative imaging of native ITAs and the postoperative study of ITA grafts before patient discharge. Repeated-measures analysis of variance was used to compare measurements of the ITA size and flow velocities (peak systolic velocity and end-diastolic velocity) at 5, 10, and 15 minutes after a single dose of sublingual nitroglycerin (0.4 mg) with the baseline values obtained without nitroglycerin. Results. The preoperative native left ITA and the postoperative left ITA graft diameters responded to sublingual nitroglycerin by showing a rapid and significant increase beginning at 5 minutes and lasting up to 15 minutes (p = 0.0001). Sublingual nitroglycerin caused the peak systolic velocity of the native left ITA to be augmented at 5 minutes (p = 0.0002), and this effect was still apparent at 10 minutes (p = 0.0001) and 15 minutes (p = 0.0192). However, postoperative left ITA graft peak systolic velocities remained unaffected by the sublingual nitroglycerin (p = not significant). Conclusions. We conclude that instantaneous noninvasive measurement of ITA graft size and blood flow velocities after a therapeutic drug intervention may be clinically useful, particularly in a post-coronary artery bypass grafting patient with recurrent angina. (C) 1997 by The Society of Thoracic Surgeons. RP Canver, CC (reprint author), UNIV WISCONSIN,WILLIAM S MIDDLETON MEM VET HOSP,SCH MED,CLIN SCI CTR H4 352,MADISON,WI 53792, USA. NR 7 TC 7 Z9 8 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0003-4975 J9 ANN THORAC SURG JI Ann. Thorac. Surg. PD APR PY 1997 VL 63 IS 4 BP 1041 EP 1043 PG 3 WC Cardiac & Cardiovascular Systems; Respiratory System; Surgery SC Cardiovascular System & Cardiology; Respiratory System; Surgery GA WT197 UT WOS:A1997WT19700027 PM 9124902 ER PT J AU Nah, EH King, DE Craig, FE AF Nah, EH King, DE Craig, FE TI CD4 and CD8 antigen coexpression - A flow cytometric study of peripheral blood, bone marrow, body fluid, and solid lymphoreticular specimens SO ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE LA English DT Article ID T-CELL LYMPHOMAS; INTERLEUKIN-4; CLONES AB Objective.-CD4 and CD8 antigen coexpression occurs not only on blastic T-cell malignancies, but also on a small subset of mature lymphocytes. The aim of this study was to determine the prevalence of this population of cells and to identify features that can be used to differentiate them from T lymphoblasts. Design.-All specimens submitted to the clinical flow cytometry laboratory from August 1, 1994, through July 31, 1995, were analyzed for CD4 and CD8 coexpression. Main Outcome Measure.-Percentage of lymphocytes coexpressing the CD4 and CD8 antigens. Results.-Four percent (22/526) of all specimens contained a population of CD4/CD8 coexpressing cells. Five cases represented CD4 and CD8 antigen expression on neoplastic cells. In 17 cases, the CD4/CD8 coexpressing cells appeared to represent a population of mature lymphocytes with a normal phenotype. The immature cells of T-cell acute lymphocytic leukemia and lymphoblastic lymphoma represented a dominant uniform population of cells demonstrating strong staining with both the CD4 and CD8 antigens. Cases containing a mature population of CD4/CD8 coexpressing cells were characterized by fewer coexpressing cells and variable expression of CD8. There were cases where distinction of this population of mature CD4/CD8 coexpressing lymphoid cells from a blastic malignancy was not possible using immunophenotyping alone. Conclusion.-Correlation of clinical, morphologic, and immunophenotypic data is recommended to prevent the misdiagnosis of subtle involvement by a blastic T-cell malignancy. C1 UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX 78384. CHONNAM UNIV HOSP,DEPT CLIN PATHOL,KWANGJU,SOUTH KOREA. AUDIE L MURPHY MEM VET ADM MED CTR,LAB SERV,SAN ANTONIO,TX 78284. NR 17 TC 2 Z9 3 U1 0 U2 0 PU COLLEGE AMER PATHOLOGISTS PI NORTHFIELD PA C/O KIMBERLY GACKI, 325 WAUKEGAN RD, NORTHFIELD, IL 60093-2750 SN 0003-9985 J9 ARCH PATHOL LAB MED JI Arch. Pathol. Lab. Med. PD APR PY 1997 VL 121 IS 4 BP 381 EP 384 PG 4 WC Medical Laboratory Technology; Medicine, Research & Experimental; Pathology SC Medical Laboratory Technology; Research & Experimental Medicine; Pathology GA WW544 UT WOS:A1997WW54400011 PM 9140307 ER PT J AU Ames, D Wirshing, WC Brammer, G Pashdag, J Yuwiler, A AF Ames, D Wirshing, WC Brammer, G Pashdag, J Yuwiler, A TI Variability in whole blood serotonin as a marker for suicidality in schizophrenia SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. PEPPERDINE UNIV,GRAD SCH EDUC & PSYCHOL,CULVER CITY,CA 90230. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 1 PY 1997 VL 41 SU 7 BP 28 EP 28 PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA WQ709 UT WOS:A1997WQ70900030 ER PT J AU Altshuler, L Bartzokis, G Grieder, T Curran, J Mintz, J AF Altshuler, L Bartzokis, G Grieder, T Curran, J Mintz, J TI Specific limbic differences in MRI of schizophrenic and bipolar subjects SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,PSYCHIAT SERV,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,RES SERV,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,DEPT PSYCHIAT,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,DEPT RADIOL,LOS ANGELES,CA 90024. RI Bartzokis, George/K-2409-2013; Mintz, Jim/N-7385-2014 OI Mintz, Jim/0000-0002-8299-5851 NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 1 PY 1997 VL 41 SU 7 BP 33 EP 33 PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA WQ709 UT WOS:A1997WQ70900035 ER PT J AU Wilkins, RT Rojas, D Teale, P Sheeder, J Aasal, R Wahlberg, LJ Marquardt, JE Sandberg, EJ Simon, J Arciniegas, D Laudenslager, M Taylor, D Montgomery, P Reite, M AF Wilkins, RT Rojas, D Teale, P Sheeder, J Aasal, R Wahlberg, LJ Marquardt, JE Sandberg, EJ Simon, J Arciniegas, D Laudenslager, M Taylor, D Montgomery, P Reite, M TI Reduced hippocampal volume in PTSD: Is it PTSD, or alcohol? SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract C1 DENVER VET AFFAIRS MED CTR,DENVER,CO 80220. UNIV COLORADO,HLTH SCI CTR,DENVER,CO 80262. RI Arciniegas, David/A-3792-2009 NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 1 PY 1997 VL 41 SU 7 BP 41 EP 41 PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA WQ709 UT WOS:A1997WQ70900043 ER PT J AU Arciniegas, D Rojas, D Sheeder, J Aasal, R Teale, P Sandberg, E Reite, M Beresford, T AF Arciniegas, D Rojas, D Sheeder, J Aasal, R Teale, P Sandberg, E Reite, M Beresford, T TI Alcohol and the pituitary in hippocampal volume loss in PTSD SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract C1 UNIV COLORADO,HLTH SCI CTR,DENVER,CO 80262. DENVER VET AFFAIRS MED CTR,DENVER,CO 80220. RI Arciniegas, David/A-3792-2009 NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 1 PY 1997 VL 41 SU 7 BP 42 EP 42 PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA WQ709 UT WOS:A1997WQ70900044 ER PT J AU Bartzokis, G Altshuler, L Grieder, T Curran, J AF Bartzokis, G Altshuler, L Grieder, T Curran, J TI Medial temporal lobe volume measurement reliability using reformatted 3D images SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,RES SERV,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,PSYCHIAT SERV,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,DEPT PSYCHIAT,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,DEPT RADIOL,LOS ANGELES,CA 90024. RI Bartzokis, George/K-2409-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 1 PY 1997 VL 41 SU 7 BP 49 EP 49 PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA WQ709 UT WOS:A1997WQ70900051 ER PT J AU Amin, F Stroe, AE Adebogun, O Silverman, JM Smith, CJ Knott, PJ Siever, LJ Davis, KL AF Amin, F Stroe, AE Adebogun, O Silverman, JM Smith, CJ Knott, PJ Siever, LJ Davis, KL TI Attenuated diurnal dopamine rhythm in non-psychotic relatives of schizophrenic probands SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract C1 BAYLOR COLL MED,HOUSTON VAMC,HOUSTON,TX 77030. MT SINAI SCH MED,BRONX VAMC,NEW YORK,NY. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 1 PY 1997 VL 41 SU 7 BP 229 EP 229 PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA WQ709 UT WOS:A1997WQ70900231 ER PT J AU Ames, D Wirshing, WC Marshall, BD Green, MF McGurk, SR Mintz, J Marder, SR AF Ames, D Wirshing, WC Marshall, BD Green, MF McGurk, SR Mintz, J Marder, SR TI Treatment resistant schizophrenia: Evaluation of risperidone vs. haloperidol SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. RI Mintz, Jim/N-7385-2014 OI Mintz, Jim/0000-0002-8299-5851 NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 1 PY 1997 VL 41 SU 7 BP 245 EP 245 PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA WQ709 UT WOS:A1997WQ70900247 ER PT J AU George, MS Speer, AM Stallings, L Wassermann, EM Kimbrell, TA Epstein, C Risch, SC Post, RM AF George, MS Speer, AM Stallings, L Wassermann, EM Kimbrell, TA Epstein, C Risch, SC Post, RM TI Combining transcranial magnetic stimulation with functional imaging SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract C1 MED UNIV S CAROLINA,DEPT PSYCHIAT,CHARLESTON,SC 29425. MED UNIV S CAROLINA,DEPT RADIOL,CHARLESTON,SC 29425. MED UNIV S CAROLINA,DEPT NEUROL,CHARLESTON,SC 29425. RALPH H JOHNSON VET AFFAIRS MED CTR,DEPT PSYCHIAT,CHARLESTON,SC. NIMH,BIOL PSYCHIAT BRANCH,BETHESDA,MD 20892. NINCDS,HUMAN MOTOR CONTROL SECT,BETHESDA,MD 20892. EMORY UNIV,DEPT NEUROL,ATLANTA,GA 30322. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 1 PY 1997 VL 41 SU 7 BP 261 EP 261 PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA WQ709 UT WOS:A1997WQ70900262 ER PT J AU Yehuda, R Siever, LJ AF Yehuda, R Siever, LJ TI Persistent effects of stress in trauma survivors and their descendants SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract C1 MT SINAI SCH MED,NEW YORK,NY 10029. BRONX VET ADM MED CTR,BRONX,NY 10468. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 1 PY 1997 VL 41 SU 7 BP 265 EP 265 PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA WQ709 UT WOS:A1997WQ70900266 ER PT J AU Roitman, SEL Keefe, RSE Mitropoulou, V Dupre, RL Siever, LJ AF Roitman, SEL Keefe, RSE Mitropoulou, V Dupre, RL Siever, LJ TI Visuospatial working memory in schizotypal personality disorder SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract C1 MT SINAI MED CTR,NEW YORK,NY 10029. BRONX VET ADM MED CTR,NEW YORK,NY. DUKE UNIV,MED CTR,DURHAM,NC 27706. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 1 PY 1997 VL 41 SU 7 BP 355 EP 355 PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA WQ709 UT WOS:A1997WQ70900355 ER PT J AU Kirrane, R Trestman, R Mitropoulou, V Nunn, M Siever, L AF Kirrane, R Trestman, R Mitropoulou, V Nunn, M Siever, L TI Effects of amphetamine on cognitive impairment in schizotypal personality disorder SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract C1 MT SINAI MED CTR,NEW YORK,NY 10029. BRONX VET ADM MED CTR,NEW YORK,NY. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 1 PY 1997 VL 41 SU 7 BP 356 EP 356 PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA WQ709 UT WOS:A1997WQ70900356 ER PT J AU Yao, JK Reddy, RD vanKammen, DP AF Yao, JK Reddy, RD vanKammen, DP TI Reduced level of the antioxidant uric acid in schizophrenia SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract C1 VA PITTSBURGH HLTH CARE SYST,PITTSBURGH,PA 15206. UNIV PITTSBURGH,WESTERN PSYCHIAT INST & CLIN,MED CTR,PITTSBURGH,PA 15213. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 1 PY 1997 VL 41 SU 7 BP 391 EP 391 PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA WQ709 UT WOS:A1997WQ70900390 ER PT J AU Yaffe, K Ettinger, B Pressman, A Seeley, D Whooley, M Schaefer, C Cummings, S AF Yaffe, K Ettinger, B Pressman, A Seeley, D Whooley, M Schaefer, C Cummings, S TI Neuropsychiatric function and DHEAS in elderly women: A prospective study SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract C1 UNIV CALIF SAN FRANCISCO,SAN FRANCISCO,CA 94143. SAN FRANCISCO VET AFFAIRES MED CTR,SAN FRANCISCO,CA. KAISER PERMANENTE MED CARE PROGRAM,DIV RES,OAKLAND,CA 94611. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 1 PY 1997 VL 41 SU 7 BP 409 EP 409 PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA WQ709 UT WOS:A1997WQ70900407 ER PT J AU WooMing, AM Trestman, RL Mitropoulou, V Siever, LJ AF WooMing, AM Trestman, RL Mitropoulou, V Siever, LJ TI Role of menstrual cycle status in noradrenergic studies SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract C1 MT SINAI VA MED CTR,NEW YORK,NY. BRONX VET ADM MED CTR,NEW YORK,NY. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD APR 1 PY 1997 VL 41 SU 7 BP 416 EP 416 PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA WQ709 UT WOS:A1997WQ70900414 ER PT J AU Durante, W Kroll, MH Christodoulides, N Peyton, KJ Schafer, AI AF Durante, W Kroll, MH Christodoulides, N Peyton, KJ Schafer, AI TI Nitric oxide induces heme oxygenase-1 gene expression and carbon monoxide production in vascular smooth muscle cells SO CIRCULATION RESEARCH LA English DT Article DE nitric oxide; carbon monoxide; heme oxygenase ID CYCLIC GUANOSINE-MONOPHOSPHATE; L-ARGININE; RAT; SYNTHASE; INDUCTION; BILIRUBIN; ENZYME; LIVER; BLOOD; INTERLEUKIN-1-BETA AB Since recent studies demonstrate that vascular smooth muscle cells synthesize two distinct guanylate cyclase-stimulatory gases, NO and CO, we examined possible regulatory interactions between these two signaling molecules. Treatment of rat aortic smooth muscle cells with the NO donors, sodium nitroprusside, S-nitroso-N-acetyl-penicillamine, or 3-morpholinosydnonimine, increased heme oxygenase-1 (HO-1) mRNA and protein levels in a concentration- and time-dependent manner. Both actinomycin D and cycloheximide blocked NO-stimulated HO-1 mRNA and protein expression. Nuclear run-on experiments demonstrated that NO donors increased HO-1 gene transcription between 3- and 6-fold. In contrast, NO donors had no effect on the stability of HO-1 mRNA. Incubation of vascular smooth muscle cells with the membrane-permeable cGMP analogues, dibutyryl cGMP and 8-bromo-cGMP, failed to induce HO-1 gene expression. Treatment of vascular smooth muscle cells with NO donors also stimulated the production and release of CO, as demonstrated by the CO-dependent increase in intracellular cGMP levels in coincubated platelets. Finally, incubating vascular smooth muscle cells with interleukin-1 beta and tumor necrosis factor-alpha induced NO synthesis and also significantly increased the level of HO-1 protein. The cytokine-stimulated production of both NO and HO-1 protein in smooth muscle cells was blocked by the NO synthase inhibitor methyl-L-arginine. These results demonstrate that exogenously administered or endogenously released NO stimulates HO-1 gene expression and CO production in vascular smooth muscle cells. The ability of NO to induce HO-catalyzed CO release from vascular smooth muscle cells provides a novel mechanism by which NO might modulate soluble guanylate cyclase and, thereby, vascular smooth muscle cell and platelet function. C1 BAYLOR COLL MED,DEPT MED,HOUSTON,TX 77030. BAYLOR COLL MED,DEPT PHARMACOL,HOUSTON,TX 77030. RP Durante, W (reprint author), HOUSTON VA MED CTR,BLDG 109,ROOM 116,2002 HOLCOMBE BLVD,HOUSTON,TX 77030, USA. FU NHLBI NIH HHS [HL-36045] NR 49 TC 252 Z9 270 U1 0 U2 3 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7330 J9 CIRC RES JI Circ.Res. PD APR PY 1997 VL 80 IS 4 BP 557 EP 564 PG 8 WC Cardiac & Cardiovascular Systems; Hematology; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Hematology GA WQ093 UT WOS:A1997WQ09300015 PM 9118487 ER PT J AU Oates, JC Ruiz, P Alexander, A Pippen, AMM Gilkeson, GS AF Oates, JC Ruiz, P Alexander, A Pippen, AMM Gilkeson, GS TI Effect of late modulation of nitric oxide production on murine lupus SO CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article ID ARTHRITIS-LIKE DISEASE; MONOMETHYL-L-ARGININE; SYNTHASE EXPRESSION; AUTOIMMUNE-DISEASE; IMMUNE-RESPONSE; MRL/1 MICE; GLOMERULONEPHRITIS; MANIFESTATIONS; ANTIBODIES; CELLS AB MRL/MpJ-Fas(lpr) (MRL-lpr) and New Zealand Black/White (NZB/W) mice develop spontaneous autoimmune disease characterized by autoantibody production and glomerulonephritis that progresses in parallel with increasing systemic nitric oxide (NO) production. A previously published study from our laboratory indicated that oral administration of the nitric oxide synthase inhibitor N-G-monomethyl-L-arginine (NMMA) before the onset of clinical disease significantly decreased renal and joint pathology in MRL-lpr mice, To characterize the effect of late modulation of NO production in murine SLE, we administered oral NMMA and/or restricted dietary arginine after disease onset in two murine models of SLE. When receiving combined NMMA and arginine restriction, MRL-Epr mice had reduced joint pathology scores and NZB/W mice had lower renal pathology scores than control mice. These results indicate that modulating NO production after the onset of disease diminishes disease severity in two models of SLE, although not as effectively as treating before disease onset. (C) 1997 Academic Press. C1 MED UNIV S CAROLINA,DEPT MED,CHARLESTON,SC 29425. UNIV MIAMI,MED CTR,MIAMI,FL 33152. DUKE UNIV,MED CTR,DURHAM,NC. RP Oates, JC (reprint author), RALPH H JOHNSON VAMC,MED RES SERV,CHARLESTON,SC 29425, USA. FU NIAMS NIH HHS [AR39162, K08 AR002193] NR 35 TC 46 Z9 48 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0090-1229 J9 CLIN IMMUNOL IMMUNOP JI Clin. Immunol. Immunopathol. PD APR PY 1997 VL 83 IS 1 BP 86 EP 92 DI 10.1006/clin.1997.4332 PG 7 WC Immunology; Pathology SC Immunology; Pathology GA WP273 UT WOS:A1997WP27300017 PM 9073540 ER PT J AU Lipsky, BA Baker, PD Landon, GC Fernau, R AF Lipsky, BA Baker, PD Landon, GC Fernau, R TI Antibiotic therapy for diabetic foot infections: Comparison of two parenteral-to-oral regimens SO CLINICAL INFECTIOUS DISEASES LA English DT Article ID LOWER-EXTREMITY INFECTIONS; SKIN-STRUCTURE; SOFT-TISSUE; OFLOXACIN; DISEASE; CIPROFLOXACIN; MANAGEMENT AB This prospective, randomized, multicenter trial compared the efficacy of two antibiotic regimens for treatment of foot infections in diabetic adults. Patients with infections requiring hospitalization were randomized to receive either intravenous ofloxacin followed by oral ofloxacin or intravenous ampicillin/sulbactam followed by oral amoxicillin/clavulanate (the aminopenicillin regimen) for 14-28 days. Patients with osteomyelitis were eligible for the study if the infected bone was to be removed. Of 108 patients enrolled in the study, 88 who were evaluable had various skin and soft-tissue infections, and 24% had osteomyelitis. For the ofloxacin and aminopenicillin regimens, the mean duration of intravenous therapy was 7.8 and 7.1 days, respectively, the mean duration of oral therapy was 13.2 and 12.0 days, respectively, the rate of eradication of pathogens was 78% and 88%, respectively, and the overall rate of clinical cure or improvement was 85% and 83%, respectively. Thus, about 3 weeks of therapy with either regimen was well tolerated and effective in treating these diabetic foot infections. C1 UNIV WASHINGTON,SCH MED,DEPT MED,DIV GEN INTERNAL MED,SEATTLE,WA 98195. HOUSTON VET AFFAIRS MED CTR,SECT ORTHOPED SURG,HOUSTON,TX. RP Lipsky, BA (reprint author), VET AFFAIRS PUGET SOUND HLTH CARE SYST,ANTIBIOT RES CLIN,1660 S COLUMBIAN WAY,SEATTLE,WA 98108, USA. OI Lipsky, Benjamin A./0000-0001-9886-5114 NR 14 TC 69 Z9 71 U1 0 U2 1 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD APR PY 1997 VL 24 IS 4 BP 643 EP 648 DI 10.1093/clind/24.4.643 PG 6 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA WT725 UT WOS:A1997WT72500015 PM 9145738 ER PT J AU Bosserhoff, AK Kondo, S Moser, M Dietz, UH Copeland, NG Gilbert, DJ Jenkins, NA Buettner, R Sandell, LJ AF Bosserhoff, AK Kondo, S Moser, M Dietz, UH Copeland, NG Gilbert, DJ Jenkins, NA Buettner, R Sandell, LJ TI Mouse CD-RAP/MIA gene: Structure, chromosomal localization, and expression in cartilage and chondrosarcoma SO DEVELOPMENTAL DYNAMICS LA English DT Article DE MIA; melanoma; CD-RAP; chondrogenesis; mouse gene ID MESSENGER-RNA; DIFFERENTIAL EXPRESSION; RAT CHONDROSARCOMA; COLLAGEN GENE; PROCOLLAGEN; PROTEIN; IDENTIFICATION; ORGANIZATION; PROPEPTIDE; SEQUENCE AB A cDNA encoding a novel protein has been previously isolated from two independent sources: melanoma cell cultures and chondrocytes. The protein from human melanoma cell lines and tumors is called melanoma inhibitory activity (MIA) (Blesch et al. [1994] Cancer Res. 54:5695-5701) and the protein from primary bovine chondrocytes and cartilaginous tissues is called cartilage-derived retinoic acid-sensitive protein (CD-RAP) (Dietz and Sandell [1996] J. Biol. Chem. 271:3311-3316). In order to investigate the gene regulation and function of CD-RAP/MIA, the mouse gene locus was isolated and analyzed, Developmental expression was determined by in situ hybridization to mouse embryos. Expression was limited to cartilaginous tissues and was initiated with the advent of chondrogenesis, remaining abundant throughout development. The mouse gene was isolated and sequenced from a 129Sv library and sequenced directly from an additional strain, B6C3Fe. The mouse CD-RAP/MIA gene is 1.5 kbp and consists of four exons. The promoter sequence of the gene contains many potential regulatory domains including 8 basic helix-loop-helix protein-binding domains and an AT-rich domain, both motifs shown to be present in the cartilage-specific enhancer of the type II procollagen gene. Other potential cis-acting motifs include binding sites for GATA-1, NF-IL6, PEA3, w-elements, NF kappa B, Zeste and Spl. The gene, called cdrap, was localized to the end of an arm of chromosome 7 at the same site as the transforming growth factor beta 1 (Tgf-beta 1) and the glucose phosphate isomerase 1 (Gpi1) genes. Potential mouse mutants that mapped to the same region of chromosome 7 mere identified. Two of the potential mutants with skeletal phenotypes were sequenced, pudgy (pu) and extra toes with spotting (Xs(J)); however, no mutations were found in the coding sequence. To determine whether CD-RAP/MIA is associated with tumors of cartilage, mRNAs from a variety of rodent tissues and cell lines were screened, Expression was detected in a rodent tumor, the Swarm rat chondrosarcoma and a chondrosarcoma cell line derived from it, but not in other tissues or tumors of non-cartilage origin. Immunolocalization revealed CD-RAP/MIA protein localized in cartilage only, These results show that the normal expression of CD-RAP/MIA is limited to cartilage; however, pathologically, it is expressed both in melanoma and chondrosarcoma. The restricted expression of CD-RAP/MIA may provide an opportunity to monitor cartilage metabolic activity as well as the tumor activity of melanoma and chondrosarcoma. (C) 1997 Wiley-Liss, Inc. C1 UNIV WASHINGTON,DEPT ORTHOPED ORT 112,VA PUGET SOUND HLTH CARE SYST,SEATTLE,WA 98108. UNIV WASHINGTON,DEPT BIOCHEM,VA PUGET SOUND HLTH CARE SYST,SEATTLE,WA 98108. UNIV REGENSBURG,DEPT PATHOL,D-93053 REGENSBURG,GERMANY. NCI,FREDERICK CANC RES & DEV CTR,MAMMALIAN GENET LAB,ABL BASIC RES PROGRAM,FREDERICK,MD 21702. FU NIAMS NIH HHS [R01AR36994] NR 31 TC 96 Z9 106 U1 1 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 1058-8388 J9 DEV DYNAM JI Dev. Dyn. PD APR PY 1997 VL 208 IS 4 BP 516 EP 525 DI 10.1002/(SICI)1097-0177(199704)208:4<516::AID-AJA7>3.0.CO;2-L PG 10 WC Anatomy & Morphology; Developmental Biology SC Anatomy & Morphology; Developmental Biology GA WQ842 UT WOS:A1997WQ84200007 PM 9097023 ER PT J AU Ho, M Marger, M Beart, J Yip, I Shekelle, P AF Ho, M Marger, M Beart, J Yip, I Shekelle, P TI Is the quality of diabetes care better in a diabetes clinic or in a general medicine clinic? SO DIABETES CARE LA English DT Article; Proceedings Paper CT 18th Annual Meeting of the National-Society-of-General-Internal-Medicine CY MAY 04-06, 1995 CL SAN DIEGO, CA SP Natl Soc Gen Internal Med ID MANAGED CARE; MELLITUS; PROTEINURIA; KIDNEY AB OBJECTIVE - To compare the quality of ambulatory diabetes care delivered by physicians in the diabetes clinic versus the general medicine clinic of a university-affiliated Veterans Administration medical center. RESEARCH DESIGN AND METHODS - This is a retrospective study that involved the review of medical records against predetermined process-of-care criteria. A total of 112 patients with diabetes were randomly selected, of whom 56 were cared for in the general medicine clinic and 56 in the diabetes clinic. The following main outcome measures were examined: 1) the compliance with individual criteria; and 2) the proportion of patient visits in each clinic receiving minimally acceptable quality, defined as a blood pressure measurement, a record of type of hypoglycemic medication, a glycated hemoglobin measurement within the past year, a urinalysis within the past year, an ophthalmologist or optometrist eye examination within the past year or scheduled in the next six months, a record of change in therapeutic management, and a scheduled return visit. RESULTS - The diabetes clinic performed significantly better than the general medicine clinic on the following criteria: a record of a patient's self-monitoring of blood glucose levels; a foot examination; a comprehensive eye examination; a glycated hemoglobin measurement; and a referral for diabetic education. The proportion of patient visits meeting the minimally acceptable levels of quality was better in the diabetes clinic than the general medicine clinic (73 vs. 52%, P = 0.02). CONCLUSIONS - Patients cared for by physicians in the diabetes clinic receive better quality of diabetes care than do patients cared for by physicians in the general medical clinic. If patient care is to be shifted from specialists to generalists, additional attention needs to be paid to ensure that generalists have the knowledge and system resources necessary to deliver an acceptable quality of diabetes care. C1 W LOS ANGELES VET AFFAIRS MED CTR, ENDOCRINOL SECT, MED SERV, LOS ANGELES, CA 90073 USA. VET ADM HLTH SERV, RES & DEV FIELD PROGRAM, CTR STUDY HEALTHCARE PROVIDER BEHAV, LOS ANGELES, CA USA. UNIV CALIF LOS ANGELES, LOS ANGELES, CA 90024 USA. RP Ho, M (reprint author), W LOS ANGELES VET AFFAIRS MED CTR, DIV GEN INTERNAL MED, 111G, GEN INTERNAL MED SECT, MED SERV, LOS ANGELES, CA 90073 USA. NR 26 TC 106 Z9 106 U1 1 U2 5 PU AMER DIABETES ASSOC PI ALEXANDRIA PA 1701 N BEAUREGARD ST, ALEXANDRIA, VA 22311-1717 USA SN 0149-5992 EI 1935-5548 J9 DIABETES CARE JI Diabetes Care PD APR PY 1997 VL 20 IS 4 BP 472 EP 475 DI 10.2337/diacare.20.4.472 PG 4 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA WP645 UT WOS:A1997WP64500003 PM 9096962 ER PT J AU Silverman, SL AF Silverman, SL TI Nasal calcitonin SO ENDOCRINE LA English DT Article DE calcitonin; osteoporosis therapy ID SALMON-CALCITONIN; POSTMENOPAUSAL OSTEOPOROSIS; ESTABLISHED OSTEOPOROSIS; HIP FRACTURE; BONE MASS; PREVENTION; ANTIBODIES; THERAPY; SPRAY AB Nasal calcitonin is a newly approved treatment for established osteoporosis that increases lumbar spine bone mass, is safe, and well tolerated. Fracture efficacy data is not yet available, although preliminary results are promising. The dose for established osteoporosis is 200 IU. The dose for prevention of postmenopausal osteoporosis has not been established. Nasal calcitonin may be analgesic to bone and may be of benefit in glucocorticoid-induced vertebral osteoporosis. Nasal spray calcitonin may be of benefit to the symptomatic patient with acute vertebral fracture, the complex patient, or the patient with established osteoporosis who is intolerant of bisphosphonates or estrogen. C1 OSTEOPOROSIS MED CTR,LOS ANGELES,CA. RP Silverman, SL (reprint author), UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,DEPT RHEUMATOL,WILSHIRE & SAWTELLE BLVD,LOS ANGELES,CA 90073, USA. NR 30 TC 5 Z9 5 U1 0 U2 1 PU HUMANA PRESS INC PI TOTOWA PA 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 SN 0969-711X J9 ENDOCRINE JI Endocrine PD APR PY 1997 VL 6 IS 2 BP 199 EP 202 DI 10.1007/BF02738965 PG 4 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA XB143 UT WOS:A1997XB14300014 PM 9225136 ER PT J AU Jacoby, RF Schlack, S Cole, CE Skarbek, M Harris, C Meisner, LF AF Jacoby, RF Schlack, S Cole, CE Skarbek, M Harris, C Meisner, LF TI A juvenile polyposis tumor suppressor locus at 10q22 is deleted from nonepithelial cells in the lamina propria SO GASTROENTEROLOGY LA English DT Article; Proceedings Paper CT Annual Meeting of the American-Gastroenterology-Association CY MAY 19-22, 1996 CL SAN FRANCISCO, CA SP Amer Gastroenterol Assoc ID COLI; CANCER; PATHOGENESIS; CARCINOMA; RISK; FISH AB Background & Aims: Juvenile polyps are characterized by an abundant lamina propria that lacks smooth muscle and may contain cystically dilated glands, with epithelium that seems normal and is nondysplastic. Rarely, an autosomal dominant inheritance pattern occurs. The aim of this study was to test the hypothesis that the genetic defect in both sporadic juvenile polyps and hereditary juvenile polyposis involves loss of function for a tumor suppressor gene. Methods: Allelic losses were detected by comparing normal DNA with tumor DNA from a series of 47 juvenile polyps from 16 patients using polymerase chain reaction amplification of microsatellite markers and fluorescent in situ hybridization (FISH). Results: Somatic deletions at 10q22 were detected in 39 of 47 juvenile polyps (83%) from 16 unrelated patients with either hereditary or sporadic juvenile polyps, and the minimum overlap localized juvenile polyposis coli to the 3-cM interval D10S219-D10S1696. Fluorescent in situ hybridization shows that the cells affected by deletion mutation reside exclusively in the lamina propria, not in the epithelium. Conclusions: The location of a novel tumor suppressor gene on chromosome 10 that is affected by deletion mutation in the majority of juvenile polyps was mapped. Unlike adenomas and carcinomas of the colonic epithelium, juvenile polyps originate in the lamina propria. C1 UNIV WISCONSIN,CTR COMPREHENS CANC,GASTROENTEROL SECT,MADISON,WI. UNIV WISCONSIN,CTR COMPREHENS CANC,DEPT MED,MADISON,WI. UNIV WISCONSIN,CTR COMPREHENS CANC,DEPT CYTOGENET,MADISON,WI. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. FU NCI NIH HHS [U01 CA59352, P30 CA14520] NR 26 TC 94 Z9 95 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 BP 1398 EP 1403 DI 10.1016/S0016-5085(97)70156-2 PG 6 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WT504 UT WOS:A1997WT50400046 PM 9098028 ER PT J AU Aleynik, S Leo, MA Takeshige, U Aleynik, M Lieber, CS AF Aleynik, S Leo, MA Takeshige, U Aleynik, M Lieber, CS TI Dilinoleoylphosphatidylcholine (DLPC) is the active antioxidant of polyenylphosphatidylcholine (PPC) SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 BRONX VET ADM MED CTR, ALCOHOL RES CTR, SECT LIVER DIS & NUTR, NEW YORK, NY USA. MT SINAI SCH MED, NEW YORK, NY USA. NR 0 TC 4 Z9 4 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A1209 EP A1209 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41904819 ER PT J AU Aleynik, S Leo, MA Aleynik, M Lieber, CS AF Aleynik, S Leo, MA Aleynik, M Lieber, CS TI Polyenylphosphatidylcholine protects rat pancreas against alcohol/iron-induced oxidative stress SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 BRONX VET ADM MED CTR, LIVER DIS SECT, NEW YORK, NY USA. BRONX VET ADM MED CTR, NUTR & ALCOHOL RES CTR, NEW YORK, NY USA. MT SINAI SCH MED, NEW YORK, NY USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A424 EP A424 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41901689 ER PT J AU Arora, S Baraona, E Lieber, CS AF Arora, S Baraona, E Lieber, CS TI Ranitidine increases blood alcohol levels in social drinkers. SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 BRONX VET ADM MED CTR, CTR ALCOHOL RES & TREATMENT, BRONX, NY USA. MT SINAI SCH MED, NEW YORK, NY USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A1214 EP A1214 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41904841 ER PT J AU Chang, L Saba, L Silverman, DHS Matin, K Mandelkern, MA Mayer, EA AF Chang, L Saba, L Silverman, DHS Matin, K Mandelkern, MA Mayer, EA TI Differences in perception and brain activation in response to visceral stimuli in IBS patient and non-patient twins. SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES, HARBOR MED CTR, DIV GASTROENTEROL, LOS ANGELES, CA 90024 USA. W LOS ANGELES VET AFFAIRS MED CTR, DEPT MED, NEUROENTER BIOL GRP, CURE, DIG DIS CTR, LOS ANGELES, CA 90073 USA. W LOS ANGELES VET AFFAIRS MED CTR, DEPT PHYSIOL, LOS ANGELES, CA 90073 USA. W LOS ANGELES VET AFFAIRS MED CTR, DEPT NUCL MED, LOS ANGELES, CA 90073 USA. NR 0 TC 3 Z9 3 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A709 EP A709 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41902827 ER PT J AU Deng, W Kaneko, H Tache, Y Mitsuma, T AF Deng, W Kaneko, H Tache, Y Mitsuma, T TI Central and peripheral actions of amylin to prevent ethanol-induced gastric injury in rats. SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES, DEPT MED,W LOS ANGELES VAMC,CURE, DIGEST DIS RES CTR, LOS ANGELES, CA 90024 USA. UNIV CALIF LOS ANGELES, BRAIN RES INST, LOS ANGELES, CA 90024 USA. AICHI MED UNIV, DEPT INTERNAL MED 4, AICHI, JAPAN. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A1141 EP A1141 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41904550 ER PT J AU Gschossmann, JM Raybould, H Ennes, H Young, S Lembo, T Mayer, EA AF Gschossmann, JM Raybould, H Ennes, H Young, S Lembo, T Mayer, EA TI Different types of cation channels mediate mechanical and chemical activation of DRG neurons SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES, SCH MED, DEPT PHYSIOL, LOS ANGELES, CA 90024 USA. W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, SCH MED,DEPT MED,CURE DIG DIS RES CTR, NEUROENTER BIOL GRP, LOS ANGELES, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A740 EP A740 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41902951 ER PT J AU Jacoby, RF Potter, D Cole, C Bosch, A AF Jacoby, RF Potter, D Cole, C Bosch, A TI Presymptomatic screening for HNPCC by detection of the phenotype of microsatellite instability in benign colon adenomas SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV WISCONSIN, DEPT MED, MADISON, WI USA. UNIV WISCONSIN, DEPT BIOSTAT, MADISON, WI USA. WILLIAM S MIDDLETON MEM VET ADM MED CTR, PATHOL & LAB MED SERV, MADISON, WI 53705 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A585 EP A585 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41902332 ER PT J AU Jacoby, RF Cole, C Clipson, L Gould, K Luongo, C Loser, A Newton, M Dove, WF AF Jacoby, RF Cole, C Clipson, L Gould, K Luongo, C Loser, A Newton, M Dove, WF TI Intestinal neoplasia in the Min mouse adenoma model is not strongly influenced by the enteric microbial status (normal flora vs germ-free) SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV WISCONSIN, SCH MED, DEPT MED, MADISON, WI USA. UNIV WISCONSIN, SCH MED, DEPT BIOSTAT, MADISON, WI USA. UNIV WISCONSIN, SCH MED, DEPT ONCOL, MADISON, WI USA. UNIV WISCONSIN, SCH MED, DEPT GENET, MADISON, WI USA. WILLIAM S MIDDLETON MEM VET ADM MED CTR, PATHOL & LAB MED SERV, MADISON, WI 53705 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A585 EP A585 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41902331 ER PT J AU Jacoby, RF Stoltenberg, RL Madsen, JA Schlack, SC Harms, BA AF Jacoby, RF Stoltenberg, RL Madsen, JA Schlack, SC Harms, BA TI Neoplasia in ileal pouch mucosa after total proctocolectomy for juvenile polyposis. SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV WISCONSIN, DEPT MED, MADISON, WI USA. UNIV WISCONSIN, DEPT ONCOL, MADISON, WI USA. UNIV WISCONSIN, DEPT GENET, MADISON, WI 53706 USA. WILLIAM S MIDDLETON MEM VET ADM MED CTR, PATHOL & LAB MED SERV, MADISON, WI 53705 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A585 EP A585 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41902330 ER PT J AU Jensen, DM Gornbein, J Jensen, ME King, J Cheng, S AF Jensen, DM Gornbein, J Jensen, ME King, J Cheng, S TI Effects of alcohol, aspirin, and other potential risk factors on outcomes of severe ulcer hemorrhage. SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES, LOS ANGELES, CA USA. W LOS ANGELES VET AFFAIRS MED CTR, CURE, HEMOSTASIS RES GRP, LOS ANGELES, CA 90073 USA. NR 0 TC 5 Z9 5 U1 0 U2 2 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A160 EP A160 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41900636 ER PT J AU Jensen, DM Cheng, S Jensen, ME Gornbein, J Jutabha, R Kovacs, TOG Machicado, GA AF Jensen, DM Cheng, S Jensen, ME Gornbein, J Jutabha, R Kovacs, TOG Machicado, GA TI Risk factors and recurrence of ulcer hemorrhage. SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR, CURE, LOS ANGELES, CA 90073 USA. NR 0 TC 5 Z9 5 U1 0 U2 2 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A160 EP A160 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41900635 ER PT J AU Kaneko, H Deng, W Mori, S Tache, Y Mitsuma, T AF Kaneko, H Deng, W Mori, S Tache, Y Mitsuma, T TI Central action of adrenomedullin (AM) to prevent ethanol-induced gastric injury is not mediated through CGRP1- and TRH-receptors in rats. SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 AICHI MED UNIV, DEPT INTERNAL MED 4, AICHI 48011, JAPAN. UNIV CALIF LOS ANGELES, BRAIN RES INST, LOS ANGELES, CA 90073 USA. W LOS ANGELES VET AFFAIRS MED CTR, CURE, DIGEST DIS RES CTR, DEPT MED, LOS ANGELES, CA 90073 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A1160 EP A1160 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41904626 ER PT J AU Kovacs, TOG Sytnik, B Walsh, JH AF Kovacs, TOG Sytnik, B Walsh, JH TI The effects of Helicobacter pylori infection on gastric distention-induced acid and gastrin secretion SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR, CURE, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, SCH MED, LOS ANGELES, CA 90024 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A183 EP A183 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41900727 ER PT J AU Kovacs, TOG Sytnik, B Walsh, JH AF Kovacs, TOG Sytnik, B Walsh, JH TI Helicobacter pylori infection decreases parietal cell sensitivity to gastrin SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR, CURE, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, SCH MED, LOS ANGELES, CA 90024 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A183 EP A183 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41900729 ER PT J AU Lembo, T Naliboff, B Saba, L Matin, K Mayer, EA AF Lembo, T Naliboff, B Saba, L Matin, K Mayer, EA TI Differential effects of somatostatin analogue and opioid on perception of rectal pain. SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES, SCH MED,DEPT MED,DIG DIS RES CTR,CURE, NEUROENTER BIOL GRP, LOS ANGELES, CA 90024 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT PHYSIOL, LOS ANGELES, CA 90024 USA. W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA 90073 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A773 EP A773 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41903082 ER PT J AU Matin, K Mayer, EA Munakata, J Naliboff, B Lembo, T AF Matin, K Mayer, EA Munakata, J Naliboff, B Lembo, T TI Intravenous octreotide (OCT) affects visceral discomfort thresholds but not stimulus-response functions in IBS patients. SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, DEPT PHYSIOL, LOS ANGELES, CA 90024 USA. UNIV CALIF LOS ANGELES, DEPT MED,SCH MED,NEUROENTER BIOL GRP, DIG DIS RES CTR,CURE, LOS ANGELES, CA 90024 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A785 EP A785 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41903129 ER PT J AU Moser, AJ Abedin, ZR Weber, P Abedin, MZ Roslyn, JJ AF Moser, AJ Abedin, ZR Weber, P Abedin, MZ Roslyn, JJ TI Na+/Ca2+ exchange by the gallbladder: A potential cause of gallstones. SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 ALLEGHENY UNIV HLTH SCI, DEPT SURG, PHILADELPHIA, PA 19102 USA. PHILADELPHIA VA MED CTR, RES SERV, PHILADELPHIA, PA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A1460 EP A1460 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41905817 ER PT J AU Munakata, J Mayer, EA FitzGerald, L Matin, K Tougas, G Naliboff, B AF Munakata, J Mayer, EA FitzGerald, L Matin, K Tougas, G Naliboff, B TI Autonomic responses to visceral stimulation are downregulated in irritable bowel syndrome (IBS) patients. SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES, SCH MED,DEPT MED,CURE DIG DIS RES CTR, NEUROENTER BIOL GRP, LOS ANGELES, CA 90024 USA. MCMASTER UNIV, HAMILTON, ON, CANADA. W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT PSYCHIAT, LOS ANGELES, CA 90024 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A794 EP A794 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41903165 ER PT J AU Munakata, J Silverman, DHS Naliboff, B Matin, K Mayer, EA AF Munakata, J Silverman, DHS Naliboff, B Matin, K Mayer, EA TI Anterior cingulate cortical activity levels during expected pain correlate with sensitized rectal perception thresholds in IBS and healthy subjects. SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES, DEPT PSYCHIAT, LOS ANGELES, CA 90024 USA. UNIV CALIF LOS ANGELES, DEPT MED,NEUROENTER BIOL GRP,CURE,DIG DIS RES CTR, LOS ANGELES, CA 90024 USA. W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA 90073 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A794 EP A794 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41903163 ER PT J AU Naliboff, B Munakata, J Matin, K FitzGerald, L Mayer, EA AF Naliboff, B Munakata, J Matin, K FitzGerald, L Mayer, EA TI Symptom severity and predominant symptom are associated with rectal sensitivity before and after sigmoid stimulation. SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES, SCH MED,DEPT MED,CURE DIG DIS RES CTR, NEUROENTER BIOL GRP, LOS ANGELES, CA 90024 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT PSYCHIAT, LOS ANGELES, CA 90024 USA. W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA 90073 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A797 EP A797 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41903175 ER PT J AU Naliboff, B Saba, L Fullerton, S Mayer, EA AF Naliboff, B Saba, L Fullerton, S Mayer, EA TI Altered sensitivity to medications in functional bowel disease SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT PSYCHIAT, LOS ANGELES, CA 90024 USA. UNIV CALIF LOS ANGELES, SCH MED,DEPT MED,CURE,DIGEST DIS RES CTR, NEUROENTER BIOL GRP, LOS ANGELES, CA 90024 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A796 EP A796 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41903174 ER PT J AU Raybould, HE Bitticaca, M Caan, S Tabrizi, Y Wong, H Walsh, JH Meyer, JH Sternini, C AF Raybould, HE Bitticaca, M Caan, S Tabrizi, Y Wong, H Walsh, JH Meyer, JH Sternini, C TI 5-HT3 receptors (5HT(5)R) localized on vagal afferents innervating the stomach and duodenum contribute to intestinal inhibition of gastric emptying. SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES, SCH MED,DEPT MED,CURE DIG DIS RES CTR, W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA 90095 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT NEUROBIOL & PHYSIOL, LOS ANGELES, CA 90095 USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A811 EP A811 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41903232 ER PT J AU Raybould, HE Tabrizi, Y Meyer, JH Tso, P AF Raybould, HE Tabrizi, Y Meyer, JH Tso, P TI Lipid-induced inhibition of gastric emptying is dependent on chylomicron formation in rats. SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES, SCH MED,DEPT MED,CURE DIG DIS RES CTR, W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA 90095 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT PHYSIOL, LOS ANGELES, CA 90095 USA. UNIV CINCINNATI, DEPT PATHOL, CINCINNATI, OH 45267 USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A811 EP A811 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41903231 ER PT J AU Seensalu, R Avedian, D Barbuti, R Slice, L Walsh, JH AF Seensalu, R Avedian, D Barbuti, R Slice, L Walsh, JH TI Inactivation of rho p21 by Cl-botulinum C3 exoenzyme enhances gastrin release from isolated canine G-cells in primary culture SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES, DEPT MED, CURE, DIGEST DIS RES CTR, LOS ANGELES, CA 90024 USA. W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA 90073 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A1187 EP A1187 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41904731 ER PT J AU Tanaka, S Engel, E Guth, PH Wenby, RB Meiselman, HJ Lee, J Kaunitz, JD AF Tanaka, S Engel, E Guth, PH Wenby, RB Meiselman, HJ Lee, J Kaunitz, JD TI Regional differences in H+, HCO3, and CO2 diffusion through native porcine gastroduodenal mucus SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES, SCH MED, DEPT MED, LOS ANGELES, CA 90024 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT BIOMATH, LOS ANGELES, CA 90024 USA. W LOS ANGELES VET AFFAIRS MED CTR, DEPT MED, LOS ANGELES, CA 90073 USA. UNIV SO CALIF, DEPT PHYSIOL & BIOPHYS, LOS ANGELES, CA 90089 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A308 EP A308 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41901228 ER PT J AU Tanaka, S Guth, PH Kaunitz, JD AF Tanaka, S Guth, PH Kaunitz, JD TI Pentagastrin gastroprotection against acid-induced injury is related to H-2 receptor activation but not acid secretion SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES, SCH MED, LOS ANGELES, CA USA. W LOS ANGELES VET AFFAIRS MED CTR, DEPT MED, LOS ANGELES, CA 90073 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A308 EP A308 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41901226 ER PT J AU Tanaka, S Chu, S Montrose, MH Kaunitz, JD AF Tanaka, S Chu, S Montrose, MH Kaunitz, JD TI Confocal microscopic study of rat esophageal interstitial pH during luminal and basolateral acid challenge SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES, SCH MED, DEPT MED, LOS ANGELES, CA 90024 USA. W LOS ANGELES VET AFFAIRS MED CTR, DEPT MED, LOS ANGELES, CA 90073 USA. JOHNS HOPKINS UNIV, SCH MED, DEPT MED, BALTIMORE, MD 21205 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A307 EP A307 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41901225 ER PT J AU Tanaka, S Engel, E Guth, PH Wenby, RB Meiselman, HJ Lee, J Kaunitz, JD AF Tanaka, S Engel, E Guth, PH Wenby, RB Meiselman, HJ Lee, J Kaunitz, JD TI CO2 and HCO3 diffusion through porcine gastric mucin SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV SO CALIF, DEPT PHYSIOL & BIOPHYS, LOS ANGELES, CA 90089 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT MED, LOS ANGELES, CA 90024 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT BIOMATH, LOS ANGELES, CA 90024 USA. W LOS ANGELES VET AFFAIRS MED CTR, DEPT MED, LOS ANGELES, CA 90073 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A307 EP A307 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41901223 ER PT J AU Yang, HT Wu, SV Song, M Pichuantes, S Nguyen, A Quan, S Polito, A Walsh, JH AF Yang, HT Wu, SV Song, M Pichuantes, S Nguyen, A Quan, S Polito, A Walsh, JH TI High prevalence of cagA, picB-positive H-pylori strains in China. SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES, SCH MED, DEPT MED, CURE, DIGEST DIS RES CTR, LOS ANGELES, CA 90024 USA. W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA 90073 USA. CHIRON CORP, EMERYVILLE, CA 94608 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1997 VL 112 IS 4 SU S BP A337 EP A337 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WV419 UT WOS:A1997WV41901345 ER PT J AU Jensen, DM Machicado, GA Hirabayashi, K AF Jensen, DM Machicado, GA Hirabayashi, K TI Prospective clinical and laboratory studies of rubber band ligators for obliteration of medium and small esophageal varices. SO GASTROINTESTINAL ENDOSCOPY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,LOS ANGELES,CA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0016-5107 J9 GASTROINTEST ENDOSC JI Gastrointest. Endosc. PD APR PY 1997 VL 45 IS 4 SU S BP 188 EP 188 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA WU214 UT WOS:A1997WU21400188 ER PT J AU Miller, YE Franklin, WA AF Miller, YE Franklin, WA TI Molecular events in lung carcinogenesis SO HEMATOLOGY-ONCOLOGY CLINICS OF NORTH AMERICA LA English DT Article ID CANCER CELL-LINE; OBSTRUCTIVE PULMONARY-DISEASE; CHROMOSOMAL REGION 3P21; NONSMALL-CELL; SHORT ARM; HOMOZYGOUS DELETION; PRENEOPLASTIC LESIONS; CIGARETTE SMOKERS; CARCINOMA; EXPRESSION AB Genetic alterations seen in established lung cancers are also often found in premalignant respiratory epithelium. The frequency, usual order, biologic consequences, and prognostic import of the alterations are only beginning to be studied. Increased knowledge regarding pulmonary premalignancy may provide earlier, more treatable endpoints for early detection and therapy. C1 DENVER VET AFFAIRS MED CTR, DIV MED ONCOL, DENVER, CO 80220 USA. UNIV COLORADO, HLTH SCI CTR, DENVER, CO USA. UNIV COLORADO, CTR CANC, DENVER, CO 80262 USA. RP DENVER VET AFFAIRS MED CTR, DIV PULM & CRIT CARE MED, 1055 CLERMONT ST, DENVER, CO 80220 USA. FU NCI NIH HHS [P30 CA46934, P50 CA58187]; NHLBI NIH HHS [R01 HL45745] NR 92 TC 8 Z9 8 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0889-8588 EI 1558-1977 J9 HEMATOL ONCOL CLIN N JI Hematol. Oncol. Clin. North Am. PD APR PY 1997 VL 11 IS 2 BP 215 EP + DI 10.1016/S0889-8588(05)70427-5 PG 1 WC Oncology; Hematology SC Oncology; Hematology GA WX529 UT WOS:A1997WX52900004 PM 9137967 ER PT J AU Bataille, R Manolagas, SC Berenson, JR AF Bataille, R Manolagas, SC Berenson, JR TI Pathogenesis and management of bone lesions in multiple myeloma SO HEMATOLOGY-ONCOLOGY CLINICS OF NORTH AMERICA LA English DT Article ID COLONY-STIMULATING FACTOR; B-CELL MALIGNANCIES; OSTEOCLAST-ACTIVATING FACTOR; LEUKEMIA INHIBITORY FACTOR; TUMOR NECROSIS FACTOR; DICHLOROMETHYLENE DIPHOSPHONATE; QUANTITATIVE HISTOLOGY; RESORBING CYTOKINE; OSTEOTROPIC FACTOR; SIGNAL TRANSDUCER AB Osteolytic lesions are among the hallmarks of multiple myeloma (MM). On the other hand, they are rare in B cell malignancies other than MM. Data have shown a close relationship between myeloma cell. growth factors and factors involved in the occurrence of bone lesions in MM. Biophosphonates, which are potent inhibitors of bone resorption to control MM-induced hypercalcemia, are of interest due to their ability to slow the progression of MM bone disease. C1 UNIV ARKANSAS MED SCI HOSP,CTR OSTEOPOROSIS & METAB BONE DIS,LITTLE ROCK,AR 72205. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA 90024. RP Bataille, R (reprint author), INST BIOL,HEMATOL LAB,9 QUAI MONCOUSU,F-44035 NANTES 01,FRANCE. NR 80 TC 45 Z9 46 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0889-8588 J9 HEMATOL ONCOL CLIN N JI Hematol. Oncol. Clin. North Am. PD APR PY 1997 VL 11 IS 2 BP 349 EP & DI 10.1016/S0889-8588(05)70435-4 PG 14 WC Oncology; Hematology SC Oncology; Hematology GA WX529 UT WOS:A1997WX52900012 PM 9137974 ER PT J AU Oberley, TD Oberley, LW AF Oberley, TD Oberley, LW TI Antioxidant enzyme levels in cancer SO HISTOLOGY AND HISTOPATHOLOGY LA English DT Review DE antioxidant enzymes; reactive oxygen species; superoxide dismutase; carcinogenesis; malignant phenotype ID MANGANESE-SUPEROXIDE-DISMUTASE; ORNITHINE DECARBOXYLASE INDUCTION; GLUTATHIONE-PEROXIDASE ACTIVITY; SYRIAN-HAMSTER TISSUES; TUMOR NECROSIS FACTOR; RENAL-CELL CARCINOMA; MOUSE-LIVER CELLS; FREE-RADICALS; MULTISTAGE CARCINOGENESIS; XANTHINE-OXIDASE AB Normal cells are protected by antioxidant enzymes from the toxic effects of high concentrations of reactive oxygen species generated during cellular metabolism. Even though cancer cells generate reactive oxygen species, it has been demonstrated biochemically that antioxidant enzyme levels are low in most animal and human cancers. However, a few cancer types have been found to have elevated levels of antioxidant enzymes, particularly manganese superoxide dismutase. Morphologic studies of animal and human cancer have confirmed that although the majority of tumor cell types from several organ systems have low antioxidant enzymes, adenocarcinomas may have elevated manganese superoxide dismutase and catalase levels. However, all cancers examined to date have some imbalance in antioxidant enzyme levels compared with the cell of origin. Antioxidant enzyme importance in cancer genesis has been difficult to evaluate in early cancerous lesions using biochemical techniques because such lesions are small and therefore below the level of detection. Using immunohistochemical techniques, early lesions of human and animal cancers were demonstrated to have low antioxidant enzymes, thus suggesting a role for these enzymes both in the genesis of cancer and the malignant phenotype. All but one human cancer cell type (the granular cell variant of human renal adenocarcinoma) examined showed both low catalase and glutathione peroxidase levels, suggesting that most cancer cell types cannot detoxify hydrogen peroxide. Our results to date are used to propose new cancer therapies based on modulation of cellular redox state. C1 UNIV WISCONSIN,SCH MED,DEPT PATHOL & LAB MED,MADISON,WI. UNIV IOWA,RADIAT RES LAB,COLL MED,IOWA CITY,IA 52242. RP Oberley, TD (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,PATHOL & LAB MED SERV,2500 OVERLOOK TERRACE,MADISON,WI 53705, USA. FU NCI NIH HHS [CA 41267]; NIDCR NIH HHS [DE 10758] NR 82 TC 257 Z9 264 U1 1 U2 12 PU F HERNANDEZ PI MURCIA PA PLAZA FUENSANTA 2-7 C, 30008 MURCIA, SPAIN SN 0213-3911 J9 HISTOL HISTOPATHOL JI Histol. Histopath. PD APR PY 1997 VL 12 IS 2 BP 525 EP 535 PG 11 WC Cell Biology; Pathology SC Cell Biology; Pathology GA WR423 UT WOS:A1997WR42300024 PM 9151141 ER PT J AU Cegielski, JP Goetz, MB Jacobson, JM Cohn, SE Weinstein, RA DeHovitz, JA Bennett, CL AF Cegielski, JP Goetz, MB Jacobson, JM Cohn, SE Weinstein, RA DeHovitz, JA Bennett, CL TI Gender differences in early suspicion of tuberculosis in hospitalized, high-risk patients during 4 epidemic years, 1987 to 1990 SO INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY LA English DT Article ID RESISTANT MYCOBACTERIUM-TUBERCULOSIS; ACQUIRED-IMMUNODEFICIENCY-SYNDROME; PNEUMOCYSTIS-CARINII PNEUMONIA; PULMONARY TUBERCULOSIS; DELAYED DIAGNOSIS; VIRUS INFECTION; HIV-INFECTION; UNITED-STATES; NEW-YORK; AIDS AB Objectives: To assess the degree to which, from 1987 to 1990, physicians suspected tuberculosis (TB) in the first 2 hospital days in human immunodeficiency virus (HIV)-infected patients with pulmonary disease. Design: Retrospective cohort study. Setting: 96 hospitals in five US cities. Patients: 2174 adult patients with acquired immunodeficiency syndrome discharged with a diagnosis of Pneumocystis carinii pneumonia from 1987 to 1990. The diagnosis generally was not known on admission. Results: Physicians suspected TB in the first 2 hospital days in 66% of these patients in 1987, a rate that increased steadily to 74% in 1990. However, the extent to which physicians considered TB among female patients decreased from 76% to 71% over the 4 years. Controlling for confounding variables by multiple logistic regression, the odds that TB would be suspected early increased 1.8-fold among men (odds ratio [OR], 1.8; 95% confidence interval [CI95], 1.4-2.4), but not in women (OR, 0.6; CI95, 0.2-1.9). Among the five cities, the odds of early suspicion of TB increased most in New York City (OR, 3.9; CI95, 2.0-7.9). Conclusions: Physicians considered TB in a timely manner in an increasing majority of male, but not female, high-risk patients during the first years of TB resurgence in the United States. Physicians must be aware of the changing epidemiology of HIV and TB, as well as their practice patterns, to prevent nosocomial transmission of this disease. C1 UNIV TEXAS,CTR HLTH,CTR PULM INFECT DIS CONTROL,TYLER,TX 75710. UNIV TEXAS,CTR HLTH,DEPT MED,TYLER,TX 75710. VET ADM MED CTR,DEPT MED,LOS ANGELES,CA. BRONX VET ADM MED CTR,DEPT MED,NEW YORK,NY. MT SINAI SCH MED,NEW YORK,NY. UNIV ROCHESTER,DEPT MED,ROCHESTER,NY. RUSH MED COLL,DEPT MED,CHICAGO,IL 60612. COOK CTY HOSP,CHICAGO,IL 60612. SUNY HLTH SCI CTR,DEPT MED,BROOKLYN,NY 11203. LAKESIDE VET ADM MED CTR,DEPT MED,CHICAGO,IL 60611. NW UNIV,CHICAGO,IL. RAND CORP,SANTA MONICA,CA. RI Bennett, Charles/C-2050-2008 OI Goetz, Matthew/0000-0003-4542-992X FU AHRQ HHS [1R01HS06494-01, 1R03HS07846-01, HS-00032032] NR 40 TC 9 Z9 10 U1 1 U2 1 PU SLACK INC PI THOROFARE PA 6900 GROVE RD, THOROFARE, NJ 08086 SN 0899-823X J9 INFECT CONT HOSP EP JI Infect. Control Hosp. Epidemiol. PD APR PY 1997 VL 18 IS 4 BP 237 EP 243 PG 7 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA WV963 UT WOS:A1997WV96300004 PM 9131365 ER PT J AU Shlaes, DM Gerding, DN John, JF Craig, WA Bornstein, DL Duncan, RA Eckman, MR Farrer, WE Greene, WH Lorian, V Levy, S McGowan, JE Paul, SM Ruskin, J Tenover, FC Watanakunakorn, C AF Shlaes, DM Gerding, DN John, JF Craig, WA Bornstein, DL Duncan, RA Eckman, MR Farrer, WE Greene, WH Lorian, V Levy, S McGowan, JE Paul, SM Ruskin, J Tenover, FC Watanakunakorn, C TI Society for Healthcare Epidemiology of America and Infectious Diseases Society of America Joint Committee on the Prevention of Antimicrobial Resistance: Guidelines for the prevention of antimicrobial resistance in hospitals SO INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY LA English DT Article ID GRAM-NEGATIVE BACILLI; STAPHYLOCOCCUS-AUREUS; ANTIBIOTIC USE; AMIKACIN USE; AMINOGLYCOSIDE RESISTANCE; CIPROFLOXACIN RESISTANCE; CEPHALOSPORIN USE; ACTIVE EFFLUX; USAGE; SURVEILLANCE AB Antimicrobial resistance results in increased morbidity, mortality, and costs of health care. Prevention of the emergence of resistance and the dissemination of resistant microorganisms will reduce these adverse effects and their attendant costs. Appropriate antimicrobial stewardship that includes optimal selection, dose, and duration of treatment, as well as control of antibiotic use, will prevent or slow the emergence of resistance among microorganisms. A comprehensively applied infection control program will interdict the dissemination of resistant strains. C1 VET AFFAIRS LAKESIDE MED CTR,CHICAGO,IL. UNIV MED & DENT NEW JERSEY,ROBERT WOOD JOHNSON MED SCH,NEW BRUNSWICK,NJ. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. SUNY HLTH SCI CTR,SYRACUSE,NY 13210. LAHEY CLIN FDN,BURLINGTON,MA. DULUTH CLIN LTD,DULUTH,MN. ST ELIZABETH HOSP,ELIZABETH,NJ. SUNY STONY BROOK,UNIV HOSP,STONY BROOK,NY 11794. BRONX LEBANON HOSP CTR,BRONX,NY 10456. TUFTS UNIV,SCH MED,BOSTON,MA 02111. GRADY MEM HOSP,ATLANTA,GA. NEW JERSEY STATE DEPT HLTH,TRENTON,NJ 08625. KAISER PERMANENTE MED CTR,LOS ANGELES,CA 90034. CTR DIS CONTROL & PREVENT,ATLANTA,GA. ST ELIZABETH HOSP,MED CTR,YOUNGSTOWN,OH 44501. RP Shlaes, DM (reprint author), WYETH AYERST RES,401 N MIDDLETOWN RD,PEARL RIVER,NY 10965, USA. RI mcgowan jr, john/G-5404-2011 NR 85 TC 164 Z9 171 U1 1 U2 3 PU SLACK INC PI THOROFARE PA 6900 GROVE RD, THOROFARE, NJ 08086 SN 0899-823X J9 INFECT CONT HOSP EP JI Infect. Control Hosp. Epidemiol. PD APR PY 1997 VL 18 IS 4 BP 275 EP 291 PG 17 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA WV963 UT WOS:A1997WV96300013 PM 9131374 ER PT J AU Samuels, MH Veldhuis, JD Kramer, P Urban, RJ Bauer, R Mundy, GR AF Samuels, MH Veldhuis, JD Kramer, P Urban, RJ Bauer, R Mundy, GR TI Episodic secretion of parathyroid hormone in postmenopausal women: Assessment by deconvolution analysis and approximate entropy SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Article ID FREQUENCY-MODULATION; CIRCADIAN-RHYTHM; PULSE AMPLITUDE; IMMUNORADIOMETRIC ASSAY; OSTEOPOROTIC PATIENTS; PULSATILE SECRETION; TRABECULAR BONE; DOWN-REGULATION; PTH-RECEPTOR; CALCIUM AB In healthy young subjects, parathyroid hormone (PTH) is secreted presumptively in a dual fashion,,vith low amplitude pulses apparently superimposed on tonic secretion, In contrast, PTH secretion has not been as well characterized in postmenopausal women, and relationships among bone density, estrogen status, and PTH release have not been explored, It is possible that a pulsatile pattern of PTH secretion is important for bone remodeling, since exogenous PTH administered in a pulsatile manner stimulates bone formation, To assess the importance of pulsatile PTH secretion as a determinant of bone mass,,ve measured PTH in blood sampled every 2 minutes for 6 h in four groups of older women: (1) high bone density receiving estrogen (n = 6), (2) high bone density not receiving estrogen (n = 5), (3) low bone density receiving estrogen (n = 6), and (4) low bone density not receiving estrogen (n = 8), The plasma PTH release profiles were subjected to deconvolution analysis, which resolves measured hormone concentrations into secretion and clearance components, and to an approximate entropy (ApEn) estimate, which provides an ensemble measure of the serial regularity or orderliness of the release process, In postmenopausal subjects, PTH was secreted in a fashion similar to that observed in young adults, with significant tonic secretion and PTH pulse occurrences averaging every 18-19 minutes, Pulsatile PTH secretion accounted for approximately 25% of the total secreted PTH, There were no differences in the amplitude or frequency of pulsatile PTH secretory parameters or in ApEn values among the four groups or compared,vith young controls, We conclude that in postmenopausal women, PTH secretory patterns and temporal organization are similar to those in healthy young subjects and are not altered in states of low bone density or estrogen deficiency, This suggests that abnormalities in orderly pulsatile PTH secretion are unlikely to play a major role in established postmenopausal osteoporosis. C1 UNIV VIRGINIA,HLTH SCI CTR,CHARLOTTESVILLE,VA. NSF,CTR BIOL TIMING,CHARLOTTESVILLE,VA. UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. UNIV TEXAS,MED BRANCH,GALVESTON,TX 77550. RP Samuels, MH (reprint author), OREGON HLTH SCI UNIV,DIV ENDOCRINOL,3181 SW SAM JACKSON PK RD,PORTLAND,OR 97201, USA. FU NCRR NIH HHS [M01-RR01346, M01-RR00334]; NICHD NIH HHS [K04-HD-00634] NR 35 TC 12 Z9 13 U1 0 U2 1 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 SN 0884-0431 J9 J BONE MINER RES JI J. Bone Miner. Res. PD APR PY 1997 VL 12 IS 4 BP 616 EP 623 DI 10.1359/jbmr.1997.12.4.616 PG 8 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA WR370 UT WOS:A1997WR37000014 PM 9101373 ER PT J AU Hadjis, TA Stevenson, WG Harada, T Friedman, PL Sager, P Saxon, LA AF Hadjis, TA Stevenson, WG Harada, T Friedman, PL Sager, P Saxon, LA TI Preferential locations for critical reentry circuit sites causing ventricular tachycardia after inferior wall myocardial infarction SO JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY LA English DT Article DE ventricular tachycardia; inferior wall myocardial infarction; reentry circuits ID HUMAN HEART; SLOW CONDUCTION; ACTIVATION; ORIGIN; ENTRAINMENT; ABLATION; ISTHMUS AB Reentrant VT Post MI. Introduction: For relatively slow monomorphic ventricular tachycardia (VT) after myocardial infarction, entrainment can be used to identify reentry circuit ''isthmus sites'' (exit sites and sites proximal to the exit) where radiofrequency (RF) catheter ablation has the greatest likelihood of interrupting reentry, Similarities in coronary and ventricular anatomy may cause such sites to form in preferential locations, The objective of this study is to determine if there are preferential locations for reentry circuit isthmus regions in chronic inferior wall infarctions causing VT. Methods and Results: Catheter mapping and RF catheter ablation was performed in 21 patients with an old inferior wall myocardial infarction and VT, The inferior wall was divided into 9 anatomic regions: 3 apical, 3 mid, and 3 basal segments, Of 46 different VTs, an endocardial isthmus site was identified in one or more zones in 28 (61%), with 10 VTs having isthmus sites in two or more adjacent regions, Isthmus zones were found in a basal region of the left ventricle in 24 (86%) of 28 VTs, in a mid segment in 9 (32%) VTs, and in an apical segment in 1 (4%) (P = 0.002), Of 30 RF current applications that terminated VT, 21 (70%) were at basal isthmus sites. Conclusion: The high prevalence of endocardial isthmus zones near the base of the left ventricle suggests that the mitral annulus often plays a role in defining the margins of reentry circuits that cause relatively slow VTs after inferior wall myocardial infarction. C1 BRIGHAM & WOMENS HOSP,DEPT MED,DIV CARDIOVASC,BOSTON,MA 02115. HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,DEPT MED,DIV CARDIOVASC,BOSTON,MA 02115. UNIV CALIF LOS ANGELES,SCH MED,W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA. NR 17 TC 34 Z9 34 U1 1 U2 3 PU FUTURA PUBL CO PI ARMONK PA 135 BEDFORD RD, PO BOX 418, ARMONK, NY 10504-0418 SN 1045-3873 J9 J CARDIOVASC ELECTR JI J. Cardiovasc. Electrophysiol. PD APR PY 1997 VL 8 IS 4 BP 363 EP 370 DI 10.1111/j.1540-8167.1997.tb00801.x PG 8 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA WT367 UT WOS:A1997WT36700001 PM 9106421 ER PT J AU McHorney, CA Haley, SM Ware, JE AF McHorney, CA Haley, SM Ware, JE TI Evaluation of the MOS SF-36 physical functioning scale (PF-10) .2. Comparison of relative precision using Likert and Rasch scoring methods SO JOURNAL OF CLINICAL EPIDEMIOLOGY LA English DT Article DE health status assessment; physical functioning; measurement precision; item response theory; Rasch rating scale analysis ID HEALTH-STATUS MEASURES; APPLYING PSYCHOMETRIC CRITERIA; QUALITY-OF-LIFE; MEDICAL REHABILITATION; PRIMARY CARE; RELIABILITY; FRAMEWORK; VALIDITY; UNIDIMENSIONALITY; REPRODUCIBILITY AB This study examined the relative precision (RP) of two methods of scoring the 10-item Physical Functioning Scale (PF-10) from a large sample of patients (n = 3445) of the Medical Outcomes Study. Based on a Likert scaling model, the PF-10 summated scoring method was compared with a Rasch Item Response Theory (IRT) scaling model in which raw scores were transformed into a latent trait variable of physical functioning. Potential differences between scoring methods were hypothesized to be attributed to: (1) the logarithmic nature of the Rasch transformation; (2) the unevenness of the PF-10 item distributions; and (3) reduction of within-group variance. RP ratios favored the Rasch model in discriminating between patients who differed in disease severity. The Rasch and Likert scoring models performed similarly for tests involving sensitivity to change over a two-year follow up period. In all comparisons, differences between methods were most apparent in clinical groups whose scores most approximated the extremes of the score distribution. Further research is necessary to test for differences between scoring models in discrimination and sensitivity to change among clinical groups whose scores are sufficiently spread across thr continuum of physical functioning, in particular patients with either very high or low physical functioning. The Rasch model of scoring may have important implications for the clinical interpretation of individual scores at all ranges of the scale. (C) 1997 Elsevier Science Inc. C1 UNIV WISCONSIN,SCH MED,DEPT PREVENT MED,MADISON,WI 53705. UNIV WISCONSIN,SCH MED,DEPT MED,MADISON,WI 53705. NEW ENGLAND REHABIL HOSP,WOBURN,MA 01801. TUFTS UNIV,NEW ENGLAND MED CTR HOSP,HLTH INST,BOSTON,MA 02111. RP McHorney, CA (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,HLTH SERV RES & DEV PROGRAM,2500 OVERLOOK TERRACE,MADISON,WI 53705, USA. RI Page, Andrew/G-5438-2012 OI Page, Andrew/0000-0003-3133-2844 NR 55 TC 142 Z9 145 U1 1 U2 10 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0895-4356 J9 J CLIN EPIDEMIOL JI J. Clin. Epidemiol. PD APR PY 1997 VL 50 IS 4 BP 451 EP 461 DI 10.1016/S0895-4356(96)00424-6 PG 11 WC Health Care Sciences & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA WX076 UT WOS:A1997WX07600011 PM 9179104 ER PT J AU Berenson, JR Rosen, L Vescio, R Lau, HS Woo, M Sioufi, A Kowalski, O Knight, RD Seaman, JJ AF Berenson, JR Rosen, L Vescio, R Lau, HS Woo, M Sioufi, A Kowalski, O Knight, RD Seaman, JJ TI Pharmacokinetics of pamidronate disodium in patients with cancer with normal or impaired renal function SO JOURNAL OF CLINICAL PHARMACOLOGY LA English DT Article ID BONE METASTASES AB Pamidronate is a second-generation bisphosphonate that undergoes negligible biodegradation and is eliminated exclusively by renal excretions. Nineteen cancer patients were stratified according to baseline creatinine clearance (Cl-cr): group I, Cl-cr >90 mL/min (n = 6); group II, Cl-cr 61 mL/min to 90 mL/min (n = 6); group III, Cl-cr 30 mL/min to 60 mL/min (n = 3); group IV, Cl-cr <30 mL/min (n = 4). All patients received a single, 90-mg dose of pamidronate disodium administered in a 4-hour intravenous infusion. Plasma and urine samples were collected at intervals up to 36 and 120 hours, respectively, after the start of infusion and were assayed for pamidronate, using validated high-performance liquid chromatography. Pamidronate's pharmacokinetics were characterized by a short distribution phase (2-3 hours) followed by rapid elimination of the drug in urine. Elimination of pamidronate was slower in patients in group IV with a mean +/- standard deviation area under the plasma concentration-time curve (AUC(0-36)) of 19.0 +/- 4.60 mu g.hr/mL compared with 8.1 +/- 3.13 mu g.hr/mL in patients in group I. A linear relationship in Cl-cr was observed for AUC(0-36) (r = 0.67), urinary excretion (r = 0.69), and renal clearance (r = 0.81). Renal clearance was proportional to Cl-cr for patients in all four renal-function groups. In the treatment of bone metastases of malignancy, successive doses of pamidronate are generally separated by weeks; thus, plasma accumulation in patients with renal impairment is not expected to be clinically relevant. A reduction in dose of pamidronate disodium should not be necessary in cancer patients with renal impairment. C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,JONSSON COMPREHENS CANC CTR,LOS ANGELES,CA 90024. DEPT CLIN DEV,RUEIL MALMAISON,FRANCE. DEPT BIOPHARMACEUT & PHARMACOKINET,RUEIL MALMAISON,FRANCE. CIBA GEIGY CORP,DEPT CLIN DEV,SUMMIT,NJ 07901. NR 11 TC 66 Z9 67 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0091-2700 J9 J CLIN PHARMACOL JI J. Clin. Pharmacol. PD APR PY 1997 VL 37 IS 4 BP 285 EP 290 PG 6 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA WU254 UT WOS:A1997WU25400003 PM 9115053 ER PT J AU Wirshing, WC Ames, D Bisheff, S Pierre, JM Mendoza, A Sun, A AF Wirshing, WC Ames, D Bisheff, S Pierre, JM Mendoza, A Sun, A TI Hepatic encephalopathy associated with combined clozapine and divalproex sodium treatment SO JOURNAL OF CLINICAL PSYCHOPHARMACOLOGY LA English DT Letter ID VALPROIC ACID; THERAPY C1 W LOS ANGELES VET AFFAIRS MED CTR,DEPT PSYCHIAT,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,DEPT PSYCHOL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,DEPT NEUROSCI,LOS ANGELES,CA 90024. RP Wirshing, WC (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,DEPT PSYCHIAT & BIOBEHAV SCI,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 14 TC 13 Z9 14 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0271-0749 J9 J CLIN PSYCHOPHARM JI J. Clin. Psychopharmacol. PD APR PY 1997 VL 17 IS 2 BP 120 EP 121 DI 10.1097/00004714-199704000-00013 PG 2 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA WQ265 UT WOS:A1997WQ26500014 PM 10950478 ER PT J AU Frazer, A AF Frazer, A TI Pharmacology of antidepressants SO JOURNAL OF CLINICAL PSYCHOPHARMACOLOGY LA English DT Article; Proceedings Paper CT Proceedings of the Satellite Symposium to the XX Collegium Internationale Neuro-Psychopharmacologicum (CINP) Congress on Neurotransmitters in Depression CY JUN 23, 1996 CL MELBOURNE, AUSTRALIA SP Collegium Int Neuro-Psychopharm (CINP) ID SEROTONIN REUPTAKE INHIBITORS; MONOAMINE-OXIDASE INHIBITORS; LOCUS-CERULEUS NEURONS; LONG-TERM TREATMENT; MAJOR DEPRESSION; DOUBLE-BLIND; ATYPICAL DEPRESSION; PHARMACOKINETIC PROPERTIES; PLASMA TRYPTOPHAN; LIMBIC FOREBRAIN AB This review covers mechanisms of action, efficacy, side effects, and toxicity of various classes of antidepressants: tricyclic antidepressants, monoamine oxidase inhibitors, second-generation antidepressants including the selective inhibitors of serotonin reuptake, and novel drugs such as mirtazapine, nefazodone, and venlafaxine. C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RP Frazer, A (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT PHARMACOL,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. FU NIMH NIH HHS [MH 29094] NR 158 TC 69 Z9 69 U1 2 U2 8 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0271-0749 J9 J CLIN PSYCHOPHARM JI J. Clin. Psychopharmacol. PD APR PY 1997 VL 17 IS 2 SU 1 BP S2 EP S18 DI 10.1097/00004714-199704001-00002 PG 17 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA WQ891 UT WOS:A1997WQ89100002 PM 9090573 ER PT J AU Vadakekalam, J Rabaglia, ME Metz, SA AF Vadakekalam, J Rabaglia, ME Metz, SA TI Roles of GTP and phospholipase C in the potentiation of Ca2+-induced insulin secretion by glucose in rat pancreatic islets SO JOURNAL OF ENDOCRINOLOGY LA English DT Article ID SENSITIVE K+ CHANNELS; MOUSE B-CELLS; PHOSPHOINOSITIDE METABOLISM; RELEASE; HYDROLYSIS; LANGERHANS; MECHANISMS; CALCIUM; CA-2+; TIME AB Glucose can augment insulin secretion independently of K+ channel closure, provided cytoplasmic free Ca2+ concentration is elevated. A role for phospholipase C (PLC) in this phenomenon has been both claimed and refuted. Recently, we have shown a role for GTP in the secretory effect of glucose as well as in glucose-induced PLC activation, using islets pre-treated with GTP synthesis inhibitors such as mycophenolic acid (MPA). Therefore, in the current studies, we examined first, whether glucose augments Ca2+-induced PLC activation and second, whether GTP is required for this effect, when K+(ATP) channels are kept open using diazoxide. Isolated rat islets pre-labeled with [H-3]myo-inositol were studied with or without first priming with glucose. There was a 98% greater augmentation of insulin secretion by 16.7 mM glucose (in the presence of diazoxide and 40 mM K+) in primed islets; however, the ability of high glucose to augment PLC activity bore no relationship to the secretory response. MPA markedly inhibited PLC in both conditions; however, insulin secretion was only inhibited (by 46%) in primed islets. None of these differences were attributable to alterations in labeling of phosphoinositides or levels of GTP or ATP. These data indicate that an adequate level of GTP is critical for glucose's potentiation of Ca2+-induced insulin secretion in primed islets but that PLC activation can clearly be dissociated from insulin secretion and therefore cannot be the major cause of glucose's augmentation of Ca2+-induced insulin secretion. C1 UNIV WISCONSIN,DEPT MED,WILLIAM S MIDDLETON MEM VET ADM HOSP,MADISON,WI 53792. UNIV WISCONSIN,ENDOCRINOL SECT,MADISON,WI 53792. FU NIDDK NIH HHS [DK 37312] NR 26 TC 11 Z9 11 U1 0 U2 2 PU J ENDOCRINOLOGY LTD PI BRISTOL PA 17/18 THE COURTYARD, WOODLANDS, ALMONDSBURY, BRISTOL, ENGLAND BS12 4NQ SN 0022-0795 J9 J ENDOCRINOL JI J. Endocrinol. PD APR PY 1997 VL 153 IS 1 BP 61 EP 71 DI 10.1677/joe.0.1530061 PG 11 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA WR278 UT WOS:A1997WR27800007 PM 9135570 ER PT J AU Boyle, LL Brownfield, MS Lent, SJ Goodman, B VoHill, H Litwin, J Carnes, M AF Boyle, LL Brownfield, MS Lent, SJ Goodman, B VoHill, H Litwin, J Carnes, M TI Intensive venous sampling of adrenocorticotropic hormone in rats with sham or paraventricular nucleus lesions SO JOURNAL OF ENDOCRINOLOGY LA English DT Article ID CORTICOTROPIN-RELEASING FACTOR; ACTH-SECRETION; VASOPRESSIN SECRETION; ARGININE VASOPRESSIN; MEDIAN-EMINENCE; RHYTHMS; BLOOD; CRF AB Adrenocorticotropic hormone (ACTH) secretion from the anterior pituitary is predominantly regulated by corticotropin-releasing hormone (CRH) and arginine vasopressin (AVP) synthesized in neurons of the paraventricular nucleus (PVN) of the hypothalamus. Secretion of ACTH occurs in pulsatile bursts. To explore the relationship between hypothalamic control and the pulsatile pattern of ACTH secretion, we measured ACTH in 2 min blood samples over 4 h in rats with intact and lesioned PVN during hypovolemic-stress or control conditions and also measured median eminence (ME) levels of CRH, AVP, and oxytocin (OT). Mean plasma ACTH was highest in the sham lesioned-hypovolemic group, lowest in the sham lesioned-control soup and intermediate in the two PVN-lesioned groups. CRH in the ME was negligible in the lesioned animals and correlated with OT and AW. Pulsatile secretion was observed despite PVN ablation. Visual inspection of composite time series suggested different temporal patterns of ACTH secretion. Principal components analysis of the individual ACTH time series revealed three significant eigenvectors which correlated differentially with the three treatment groups. Neither lesioned group had the steep rise over 10 min seen in plasma ACTH in the non-lesioned groups. Delayed ACTH rise after 30-60 min occurred in all but the sham control group. Our data suggest that CRH is responsible for immediate secretion of ACTH in response to hypovolemic stress and that regulators from non-PVN sites may be responsible for more delayed secretion of ACTH in this setting. The persistence of ME AVP and OT levels in the face of >90% reduction in ME CRH levels leaves open the question of a role for one or both of these peptides in the delayed ACTH response following stress onset and in the generation of pulsatile ACTH secretory bursts. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,DEPT MED,MADISON,WI 53705. WILLIAM S MIDDLETON MEM VET ADM MED CTR,CTR CLIN,MADISON,WI 53705. UNIV WISCONSIN,COLL AGR & LIFE SCI,MADISON,WI 53706. UNIV WISCONSIN,SCH VET MED,MADISON,WI 53706. UNIV WISCONSIN,DEPT MED,MADISON,WI 53706. FU NIDDK NIH HHS [R29DK40759] NR 24 TC 11 Z9 11 U1 0 U2 0 PU J ENDOCRINOLOGY LTD PI BRISTOL PA 17/18 THE COURTYARD, WOODLANDS, ALMONDSBURY, BRISTOL, ENGLAND BS12 4NQ SN 0022-0795 J9 J ENDOCRINOL JI J. Endocrinol. PD APR PY 1997 VL 153 IS 1 BP 159 EP 167 DI 10.1677/joe.0.1530159 PG 9 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA WR278 UT WOS:A1997WR27800018 PM 9135580 ER PT J AU Kirikae, T Kirikae, F Tominaga, K Qureshi, N Yamamoto, S Nakano, M AF Kirikae, T Kirikae, F Tominaga, K Qureshi, N Yamamoto, S Nakano, M TI Rhodobacter sphaeroides diphosphoryl lipid A inhibits interleukin-6 production in CD14-negative murine marrow stromal ST2 cells stimulated with lipopolysaccharide or paclitaxel (taxol) SO JOURNAL OF ENDOTOXIN RESEARCH LA English DT Article ID TUMOR-NECROSIS-FACTOR; MONOCYTE DIFFERENTIATION ANTIGEN; RHODOPSEUDOMONAS-SPHAEROIDES; BINDING-PROTEIN; SOLUBLE CD14; BACTERIAL LIPOPOLYSACCHARIDE; NONTOXIC LIPOPOLYSACCHARIDE; TYROSINE PHOSPHORYLATION; ENDOTHELIAL-CELLS; FACTOR-ALPHA AB Paclitaxel (taxol), a microtubule stabilizer with anticancer activity, mimics the actions of lipopolysaccharide (LPS) on murine macrophages in vitro. Recent studies have shown that the Rhodobacter sphaeroides diphosphoryl lipid A (RsDPLA) inhibits both LPS- and paclitaxel-induced activation of murine macrophages, and have suggested that LPS, RsDPLA, and paclitaxel share the same receptor site on murine macrophages. To analyze this receptor site, the present study focused on the interactions between LPS, RsDPLA and paclitaxel in the activation of ST2 cells derived from murine bone marrow stroma. The ST2 cells did not express CD14 mRNA. The cells produced IL-6 molecules and expressed IL-6 mRNA in response to LPS, but did not produce TNF and nitric oxide. Paclitaxel induced IL-6 mRNA expression in ST2 cells. RsDPLA inhibited both LPS- and paclitaxel-induced IL-6 mRNA expression in a dose-dependent manner. These results suggest that LPS, RsDPLA, and paclitaxel are recognized by the same receptor complex on ST2 cells, and that the receptor functions without membrane CD14. C1 UNIV WISCONSIN,WILLIAM S MIDDLETON MEM VET HOSP,MYCOBACTERIOL RES LAB,MADISON,WI. UNIV WISCONSIN,COLL AGR & LIFE SCI,DEPT BACTERIOL,MADISON,WI. OITA MED UNIV,DEPT PATHOL,OITA,JAPAN. RP Kirikae, T (reprint author), JICHI MED SCH,DEPT MICROBIOL,MINAMI KAWACHI,TOCHIGI 32904,JAPAN. NR 56 TC 8 Z9 8 U1 0 U2 0 PU CHURCHILL LIVINGSTONE PI EDINBURGH PA JOURNAL PRODUCTION DEPT, ROBERT STEVENSON HOUSE, 1-3 BAXTERS PLACE, LEITH WALK, EDINBURGH EH1 3AF, MIDLOTHIAN, SCOTLAND SN 0968-0519 J9 J ENDOTOXIN RES JI J. Endoxtin Res. PD APR PY 1997 VL 4 IS 2 BP 115 EP 122 PG 8 WC Biochemistry & Molecular Biology; Immunology; Medicine, Research & Experimental; Microbiology SC Biochemistry & Molecular Biology; Immunology; Research & Experimental Medicine; Microbiology GA XF565 UT WOS:A1997XF56500005 ER PT J AU Passman, LJ Garcia, RE Campbell, L Winter, E AF Passman, LJ Garcia, RE Campbell, L Winter, E TI Elderly veterans receiving care at a Veterans Affairs Medical Center while enrolled in Medicare-financed HMOs - Is the taxpayer paying twice? SO JOURNAL OF GENERAL INTERNAL MEDICINE LA English DT Article; Proceedings Paper CT 19th Annual Meeting of the Society-of-General-Internal-Medicine CY MAY 02-04, 1996 CL WASHINGTON, DC SP Soc Gen Internal Med DE veterans; elderly; Medicare; HMOs; health care financing AB Elderly veterans who visit our Veterans Affairs (VA) Medical Center primary care clinic often mention they are enrolled in HMOs. Approximately 20% of patients hospitalized at our facility report health insurance coverage. Of 1,000 hospitalizations during a 6-month period in which veterans reported insurance coverage, 337 involved elderly veterans. Of these 337 hospitalizations, 218 (65%) were for 174 veterans who stated they were enrolled in a Medicare-financed HMO. The VA's Medical Care Cost Recovery Program deemed only 46 (21%) of the hospitalizations billable and received reimbursement for 20 (9%). Thus, the VA is providing costly services already paid for by the Health Care Financing Administration under prepaid capitation contracts, and recovers minimal reimbursement from the HMOs. RP Passman, LJ (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,DEPT MED W111G,DIV GEN INTERNAL MED,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 7 TC 29 Z9 29 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 SN 0884-8734 J9 J GEN INTERN MED JI J. Gen. Intern. Med. PD APR PY 1997 VL 12 IS 4 BP 247 EP 249 DI 10.1007/s11606-006-5048-y PG 3 WC Health Care Sciences & Services; Medicine, General & Internal SC Health Care Sciences & Services; General & Internal Medicine GA WU682 UT WOS:A1997WU68200008 PM 9127230 ER PT J AU Carter, RH Doody, GM Bolen, JB Fearon, DT AF Carter, RH Doody, GM Bolen, JB Fearon, DT TI Membrane IgM-induced tyrosine phosphorylation of CD19 requires a CD19 domain that mediates association with components of the B cell antigen receptor complex SO JOURNAL OF IMMUNOLOGY LA English DT Article ID SIGNAL-TRANSDUCTION MOLECULE; LYMPHOCYTES-B; T-CELL; PHOSPHATIDYLINOSITOL 3-KINASE; CYTOPLASMIC DOMAIN; CR2/CD19 COMPLEX; ACTIVATION; CD22; VAV; DIFFERENTIATION AB CD19 enhances membrane IgM (mIgM) signaling and is required for B lymphocyte responses to T-dependent Ags, CD19 is tyrosine phosphorylated when mIgM is ligated and binds SH2 domain-containing signaling proteins, We suggest that the basis for phosphorylation is the association of CD19 with Syk and other components of the mIgM complex, IgM, CD22, Ig-alpha, Ig-beta, and Syk were coimmunoprecipitated with CD19 from detergent lysates of B lymphocytes. The association was maintained with a chimeric form of CD19 containing only the transmembrane domain and the membrane proximal 17 amino acids of the cytoplasmic domain encoded by exon 6. This sequence is sufficient to mediate the association, as a synthetic peptide of the exon 6-encoded region adsorbs IgM and Syk, Deletion of the juxtamembrane 17 amino acids of the cytoplasmic domain encoded by CD19 exon 6 abolishes association of CD19 with the mIgM complex. Deletion of these amino acids, which contain no tyrosines, also reduces mIgM-induced tyrosine phosphorylation of the remainder of the CD19 cytoplasmic domain, Coligating this mutant CD19 to mIgM restores phosphorylation. Thus, a discrete region of the cytoplasmic domain regulates the tyrosine phosphorylation of CD19 in the activation of B cells by mIgM. C1 UNIV ALABAMA,DEPT MICROBIOL,BIRMINGHAM,AL 35294. BIRMINGHAM VET AFFAIRS MED CTR,BIRMINGHAM,AL. UNIV CAMBRIDGE,SCH CLIN MED,WELLCOME TRUST IMMUNOL UNIT,CAMBRIDGE CB2 1TN,ENGLAND. DNAX RES INST MOL & CELLULAR BIOL INC,PALO ALTO,CA 94304. RP Carter, RH (reprint author), UNIV ALABAMA,DEPT MED,409 LHRB,701 S 19TH ST,BIRMINGHAM,AL 35294, USA. FU Wellcome Trust NR 33 TC 65 Z9 66 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 1997 VL 158 IS 7 BP 3062 EP 3069 PG 8 WC Immunology SC Immunology GA WQ643 UT WOS:A1997WQ64300005 PM 9120258 ER PT J AU Aranda, R Sydora, BC McAllister, PL Binder, SW Yang, HY Targan, SR Kronenberg, M AF Aranda, R Sydora, BC McAllister, PL Binder, SW Yang, HY Targan, SR Kronenberg, M TI Analysis of intestinal lymphocytes in mouse colitis mediated by transfer of CD4(+), CD45RB(high) T cells to SCID recipients SO JOURNAL OF IMMUNOLOGY LA English DT Article ID INFLAMMATORY BOWEL-DISEASE; MUCOSAL IMMUNE-SYSTEM; INTRAEPITHELIAL LYMPHOCYTES; MONOCLONAL-ANTIBODY; MICE; INTERLEUKIN-8; CYTOKINES; INDUCE AB Transfer of specific T lymphocyte subsets isolated from the spleens of healthy donor mice into immunodeficient SCID mice leads to chronic intestinal inflammation with characteristics similar to those of human inflammatory bowel disease (IBD). CD4(+) CD45RB(high) cells cause disease, whereas CD4(+), CD45RB(low) and CD8(+) CD45RB(high) cells do not. Despite this difference, we demonstrate that all three T cell populations reconstitute the intraepithelial and lamina propria compartments of both small and large intestines of SCID recipients. Therefore, infiltration of lymphocytes alone is not sufficient for disease development. CD4(+) lymphocytes that have trafficked to the SCID intestine exhibit a phenotype characteristic of normal mucosal lymphocytes. This includes high expression of alpha(E) integrin and CD69, expression of CD8 alpha alpha homodimers in some of the intraepithelial lymphocytes, as well as low expression of CD62L and CD45RB. The phenotype of the infiltrating mucosal cells is indistinguishable, with respect to the cell surface markers tested, regardless of whether the starting donor population is CD45RB(high) or CD45RB(low). Severe inflammation is restricted primarily to the colon despite lymphocyte infiltration throughout the length of the intestine. This suggests that some property of the colon microenvironment contributes to inflammation. Consistent with this, transfer of CD4(+), CD45RB(high) cells to SCID mice that have significantly reduced numbers of enteric flora results in attenuation of the wasting and colitis. Fewer numbers of donor lymphocytes are recovered from the intraepithelial and lamina propria compartments of reduced flora SCID mice. We hypothesize that the ability of pathogenic cells to traffic to the intestine and mediate colitis may be driven by T cell reactivity to bacteria or bacterial products. C1 UNIV CALIF LOS ANGELES,INST MOL BIOL,LOS ANGELES,CA 90095. W LOS ANGELES VET AFFAIRS MED CTR,DEPT GASTROENTEROL,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,DEPT MICROBIOL & IMMUNOL,LOS ANGELES,CA 90095. MIDWAY HOSP MED CTR,LOS ANGELES,CA 90019. CEDARS SINAI MED CTR,DEPT MED,DIV MED GENET,LOS ANGELES,CA 90048. CEDARS SINAI MED CTR,DEPT PATHOL,LOS ANGELES,CA 90048. CEDARS SINAI MED CTR,CTR INFLAMMATORY BOWEL DIS,LOS ANGELES,CA 90048. CEDARS SINAI MED CTR,DIV GASTROENTEROL,LOS ANGELES,CA 90048. RI Ain, Kenneth/A-5179-2012 OI Ain, Kenneth/0000-0002-2668-934X FU NIDDK NIH HHS [P01 DK46763] NR 25 TC 229 Z9 234 U1 0 U2 2 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD APR 1 PY 1997 VL 158 IS 7 BP 3464 EP 3473 PG 10 WC Immunology SC Immunology GA WQ643 UT WOS:A1997WQ64300055 PM 9120308 ER PT J AU Pekary, AE Levin, SR Johnson, DG Berg, L Hershman, JM AF Pekary, AE Levin, SR Johnson, DG Berg, L Hershman, JM TI Tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta 1 (TGF-beta 1) inhibit the expression and activity of Na+/K+-ATPase in FRTL-5 rat thyroid cells SO JOURNAL OF INTERFERON AND CYTOKINE RESEARCH LA English DT Article ID BETA-SUBUNIT; RECEPTOR; PROTEIN; IODIDE; GENES; DNA; RNA AB We recently reported that tumor necrosis factor-alpha (TNF-alpha) induction of the synthesis and secretion of transforming growth factor (TGF)-beta 1 by FRTL-5 cells is a thyroid-stimulating hormone (TSH)-dependent and age-dependent process.((9)) TNF-alpha is only cytotoxic to aged (>40 passages) FRTL-5 cells grown in TSH-containing medium, whereas TGF-beta induces programmed cell death (apoptosis) in epithelial cells but not in FRTL-5 cells, which otherwise retain many properties of normal thyroid follicular cells. This cell line is, therefore, a convenient model for studies on the TSH-dependent and age-dependent inhibitory effects of these cytokines on epithelial cell growth, viability, and function. One prominent effect of TNF-alpha (and TGF-beta 1) on FRTL-5 cell function is suppression of iodide uptake, which is markedly stimulated by TSH. In aged FRTL-5 cells, iodide uptake is only about 10% that of young control cells, Na+/K+-ATPase activity, which drives iodide uptake by thyroid cells, is inhibited by TNF-alpha and TGF-beta. The following experiments quantitate the effects of TSH, aging, TNF-alpha and TGF-beta 1 on the expression and activity of Na+/K+-ATPase activity in FRTL-5 cells. Young (<20 passages) and aged (>40 passages) FRTL-5 cells were treated with various doses (0-100 ng/ml) of recombinant human TNF-alpha or TGF-beta 1 for various times (0-3 days) with and without 2 U/liter TSH. These treatments reduced the rate-limiting Na+/K+-ATPase beta 1 mRNA level and Na+/K+-ATPase activity in parallel in a dose-dependent and time-dependent fashion, Aged FRTL-5 cells were more sensitive to the inhibitory effects of TNF-alpha, whereas young cells were more sensitive to the suppressive effects of TGF-beta 1 on the expression and activity of Na+/K+-ATPase, We conclude that inhibition of Na+/K+-ATPase activity by TNF-alpha and TGF-beta in FRTL-5 cells is differentially affected by aging and that this inhibitory effect can be dissociated from effects on cell viability. C1 W LOS ANGELES VET AFFAIRS MED CTR,DIABET RES LABS,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA 90073. RP Pekary, AE (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,ENDOCRINOL RES LABS,ENDOCRINOL & METAB SECT,LOS ANGELES,CA 90073, USA. NR 43 TC 15 Z9 16 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 1079-9907 J9 J INTERF CYTOK RES JI J. Interferon Cytokine Res. PD APR PY 1997 VL 17 IS 4 BP 185 EP 195 DI 10.1089/jir.1997.17.185 PG 11 WC Biochemistry & Molecular Biology; Cell Biology; Immunology SC Biochemistry & Molecular Biology; Cell Biology; Immunology GA WX550 UT WOS:A1997WX55000003 PM 9142647 ER PT J AU Werth, VP AF Werth, VP TI Dapsone as a glucocorticoid-sparing agent in pemphigus vulgaris. SO JOURNAL OF INVESTIGATIVE DERMATOLOGY LA English DT Meeting Abstract C1 UNIV PENN,DEPT DERMATOL,PHILADELPHIA,PA 19104. PHILADELPHIA VA MED CTR,PHILADELPHIA,PA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 SN 0022-202X J9 J INVEST DERMATOL JI J. Invest. Dermatol. PD APR PY 1997 VL 108 IS 4 BP 722 EP 722 PG 1 WC Dermatology SC Dermatology GA WP040 UT WOS:A1997WP04000746 ER PT J AU Aurang, K Wang, J Lloyd, KCK AF Aurang, K Wang, J Lloyd, KCK TI Somatostatin inhibition of acid and histamine release by activation of somatostatin receptor subtype 2 receptors in rats SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID ENTEROCHROMAFFIN-LIKE CELLS; MONOCLONAL-ANTIBODY IMMUNONEUTRALIZATION; CANINE PARIETAL-CELLS; GASTRIC-ACID; FUNCTIONAL-CHARACTERIZATION; FUNDIC SOMATOSTATIN; ANESTHETIZED RATS; PRIMARY CULTURE; MUCOSAL CELLS; SECRETION AB Peptide analogs of somatostatin with relatively selective binding affinities for specific somatostatin receptor subtypes, including SMS-201-995 [somatostatin receptor subtype (sst)(2), sst(3) and sst(5)], NC-8-12 (sst(2)), BIM-23058 (sst(3)) and BIM-23052 (sst(5)), were administered i.v. to anesthetized rats to determine the somatostatin receptor subtypes involved in regulation of acid secretion stimulated by either pentagastrin (24 mu g/kg/hr), bethanechol (0.2 mg/kg/hr) or histamine (5 mg/kg/hr) and in regulation of histamine release stimulated by either pentagastrin or bethanecol. Somatostatin-14 (10 nmol/kg/hr) inhibited pentagastrin-stimulated and bethanecol-stimulated acid secretion to basal levels but inhibited histamine-stimulated secretion to just 68% of maximum. SMS-201-995 (10 nmol/kg/hr) inhibited acid secretion similarly to somatostatin-14, indicating that activation of sst(2), sst(3) and/or sst(5) receptors accounts for acid inhibition induced by somatostatin. NC-8-12 dose-dependently (0.1, 1, 10 and 100 nmol/kg/hr) inhibited acid secretion stimulated by pentagastrin and by bethanecol, but only the highest dose administered (100 nmol/kg/hr) blocked by half the acid response to histamine; BIM-23058 and BIM-23052 were significantly less effective. NC-8-12 (60 +/- 12% of maximum) and somatostatin-14 (50 +/- 14% of maximum) also blocked pentagastrin-stimulated histamine release, whereas BIM-23058 and BIM-23052 were ineffective. None of the agonists significantly reduced bethanecol-stimulated histamine release. These results indicate that somatostatin activation of sst(2) receptors is the principal physiological pathway for somatostatin-induced inhibition of gastric acid secretion stimulated by either pentagastrin, bethanecol or histamine and of pentagastrin-stimulated histamine release. C1 UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,MED RES SERV,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,DEPT MED,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,DEPT PHYSIOL,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,SCH MED,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,CURE VA,DIGEST DIS RES CTR,LOS ANGELES,CA 90073. FU NIDDK NIH HHS [DK41301, DK45752] NR 63 TC 26 Z9 26 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-3565 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD APR PY 1997 VL 281 IS 1 BP 245 EP 252 PG 8 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA WU522 UT WOS:A1997WU52200031 PM 9103503 ER PT J AU Mickelson, JK Blum, CM Geraci, JM AF Mickelson, JK Blum, CM Geraci, JM TI Acute myocardial infarction: Clinical characteristics, management and outcome in a metropolitan Veterans Affairs Medical Center teaching hospital SO JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY LA English DT Article ID CORONARY-ARTERY DISEASE; NON-Q-WAVE; ISCHEMIC-HEART-DISEASE; THROMBOLYTIC THERAPY; UNITED-STATES; CARDIOVASCULAR PROCEDURES; NATIONAL-REGISTRY; BLACK PERSONS; SURGERY; SURVIVAL AB Objectives. The influence of race and age on thrombolytic therapy, invasive cardiac procedures and outcomes was assessed in a Veterans Affairs teaching hospital. The influence of Q wave evolution on the use of invasive cardiac procedures and outcome was also assessed. Background. It is not well known how early revascularization procedures for acute myocardial infarction are delivered or influence survival in a Veterans Affairs patient population. Methods. From October 1993 to October 1995, all patients with myocardial infarction were identified by elevated creatine kinase, MB fraction (CK-MB) and one of the following: chest pain or shortness of breath during the preceding 24 h or electrocardiographic (EGG) abnormalities. Results. Racial groups were similar in terms of age, time to EGG, peak CK and length of hospital stay. Mortality increased with age (odds ratio [OR] 1.93, 95% confidence interval [CI] 1,33 to 2.81). A trend toward increased mortality occurred for race other than Caucasian. Patients meeting ECG criteria were given thrombolytic agents in 49% of cases, but age, comorbidity count and Hispanic race decreased the probability of thrombolytic use. Cardiac catheterization was performed more often after thrombolytic agents (OR 1.85, 95% CI 0.97 to 3.54), but less often in African-Americans (OR 0.59, 95% CI 0.35 to 1.02), older patients (OR 0.39, 95% CI 0.24 to 0.64) or patients with heart failure (OR 0.30, 95% CI 0.17 to 0.52). Patients evolving non-Q wave infarctions were older and had increased comorbidity counts and trends toward increased mortality. Angioplasty was chosen less for patients greater than or equal to 65 years old (p = 0.02); angioplasty and coronary artery bypass graft surgery were performed less in patients greater than or equal to 70 years old (p = 0.02). Patients treated invasively had lower mortality rates than those treated medically (p < 0.02). Conclusions. The use of thrombolytic agents and invasive treatment plans declined with age, and mortality increased with age. Trends toward increased mortality occurred with non-Q wave infarctions and race other than Caucasian. C1 BAYLOR COLL MED,DEPT MED,SECT CARDIOL & GEN INTERNAL MED,HOUSTON,TX 77030. RP Mickelson, JK (reprint author), HOUSTON VET AFFAIRS MED CTR,2002 HOLCOMBE BLVD,HOUSTON,TX 77030, USA. NR 54 TC 33 Z9 33 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0735-1097 J9 J AM COLL CARDIOL JI J. Am. Coll. Cardiol. PD APR PY 1997 VL 29 IS 5 BP 915 EP 925 PG 11 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA WR446 UT WOS:A1997WR44600006 PM 9120176 ER PT J AU Ghusn, HF Teasdale, TA Jordan, D AF Ghusn, HF Teasdale, TA Jordan, D TI Continuity of do-not-resuscitate orders between hospital and nursing home settings SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Article ID ADVANCE DIRECTIVES; CARE AB OBJECTIVE: To determine the relationship between interinstitutional communication and continuity of advance directives from hospital to nursing home (NH) settings. DESIGN: Retrospective chart review of discharges to hospital affiliated or community NHs. SETTING: Teaching Veterans Affairs Hospital and affiliated and community nursing homes. MEASUREMENTS: Demographic characteristics, medical diagnoses, presence of advance directives, and documentation that relates to the topic. RESULTS: A total of 83 patients were discharged to either setting. Before discharge to a NH, the prevalence of chronic obstructive pulmonary disease and cancer was higher among those who had a DNR order. Overall, subsequent discussions about advance directives were equally common in NHs. Having a hospital discussion about advance directives or having a hospital DNR order were associated with a higher rate of advance directive discussions in NHs. Hospital DNR orders were continued for 93% and 41% of patients admitted to the hospital-affiliated NH compared with community NHs, respectively (P < .001). Specific communication of hospital DNR status to the receiving NH was associated with better continuity of DNR orders (49% vs 9%, P = .001). Factors that predicted continuity of DNR orders in logistic regression analysis correctly included hospital DNR status, communication of advance directives to the receiving NH, and NH advance directive discussions. CONCLUSIONS: There is higher continuation rate of DNR orders between the hospital under study and its affiliated NH than to community NHs despite a similar frequency of confirmation discussions. Completing advance directives before patients are discharged to NHs, communication of advance directives to the receiving NH, and follow-up discussions at the NH may improve the continuity of advance directives between hospitals and nursing homes. C1 HOUSTON VET AFFAIRS MED CTR,NURSING SERV,HOUSTON,TX 77030. BAYLOR COLL MED,DEPT MED,HOUSTON,TX 77030. RP Ghusn, HF (reprint author), HOUSTON VET AFFAIRS MED CTR,GERIATR & EXTENDED CARE SERV 110,2002 HOLCOMBE BLVD,HOUSTON,TX 77030, USA. NR 9 TC 15 Z9 15 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 1997 VL 45 IS 4 BP 465 EP 469 PG 5 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA WT160 UT WOS:A1997WT16000011 PM 9100716 ER PT J AU Bruskewitz, RC Reda, DJ Wasson, JH Barrett, L Phelan, M AF Bruskewitz, RC Reda, DJ Wasson, JH Barrett, L Phelan, M TI Testing to predict outcome after transurethral resection of the prostate SO JOURNAL OF UROLOGY LA English DT Article DE prostatectomy; prostate; outcome assessment (health care); prostatic hypertrophy ID HYPERPLASIA AB Purpose: We assessed the ability of routine clinical tests to predict outcome following transurethral resection of the prostate. Materials and Methods: A total of 556 men randomized into a trial of surgery versus watchful waiting was evaluated preoperatively with symptom interview, quality of life assessment, uro-flowmetry, urinalysis, standard chemistry panel, post-void residual urine determination and cystoscopy. The ability to predict avoidance of postoperative complications, and improvement in quality of life and genitourinary symptoms was assessed in the 249 men randomized to undergo transurethral resection of the prostate. Results: Patients with the highest symptom scores were most likely to have symptom improvement and those most bothered by the symptoms were most likely to have improvement in quality of life. No objective tests measuring physiological parameters made clinically significant contributions toward predicting these outcomes. Lower obstructive symptom scores and larger perioperative infusions of intravenous fluids were associated with a greater chance of complications. Conclusions: Symptom analysis and quality of life assessment are most useful in selecting patients for transurethral resection of the prostate. Objective diagnostic tests are of limited additional benefit. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,DEPT SURG,MADISON,WI. VET ADM MED CTR,COOPERAT STUDIES PROGRAM,HINES,IL. VET ADM MED CTR,WHITE RIVER JCT,VT. NR 5 TC 22 Z9 22 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-5347 J9 J UROLOGY JI J. Urol. PD APR PY 1997 VL 157 IS 4 BP 1304 EP 1308 DI 10.1016/S0022-5347(01)64958-9 PG 5 WC Urology & Nephrology SC Urology & Nephrology GA WN129 UT WOS:A1997WN12900037 PM 9120927 ER PT J AU Rowe, CL Baker, SC Nathan, MJ Fleming, JO AF Rowe, CL Baker, SC Nathan, MJ Fleming, JO TI Evolution of mouse hepatitis virus: Detection and characterization of spike deletion variants during persistent infection SO JOURNAL OF VIROLOGY LA English DT Article ID MURINE CORONAVIRUS JHM; CENTRAL-NERVOUS-SYSTEM; QUASI-SPECIES NATURE; RNA RECOMBINATION; NEUROTROPIC CORONAVIRUS; MONOCLONAL-ANTIBODIES; SEQUENCE-ANALYSIS; GLYCOPROTEIN; NEUROVIRULENCE; RATS AB High-frequency RNA recombination has been proposed as an important mechanism for generating viral deletion variants of murine coronavirus. Indeed, a number of variants with deletions in the spike glycoprotein have been isolated from persistently infected animals. However, the significance of generating and potentially accumulating deletion variants in the persisting viral RNA population is unclear. To study this issue, we evaluated the evolution of spike variants by examining the population of spike RNA sequences detected in the brains and spinal cords of mice inoculated with coronavirus and sacrificed at 4, 42, or 100 days postinoculation. We focused on the S1 hypervariable region since previous investigators had shown that this region is subject to recombination and deletion. RNA isolated from the brains or spinal cords of infected mice was rescued by reverse transcription-PCR, and the amplified products were cloned and used in differential colony hybridizations to identify individual isolates with deletions. We found that 11 of 20 persistently infected mice harbored spike deletion variants (SDVs), indicating that deletions are common but not required for persistent infection. To determine if a specific type of SDV accumulated during persistence, we sequenced 106 of the deletion isolates. We identified 23 distinct patterns of SDVs, including 5 double-deletion variants. Furthermore, we found that each mouse harbored distinct variants in its central nervous system (CNS), suggesting that SDVs are generated during viral replication in the CNS. Interestingly, mice with the most severe and persisting neurological disease harbored the most prevalent and diverse quasispecies of SDVs. Overall, these findings illustrate the complexity of the population of persisting viral RNAs which may contribute to chronic disease. C1 UNIV WISCONSIN,DEPT NEUROL,MADISON,WI 53792. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI 53792. UNIV WISCONSIN,DEPT MED MICROBIOL,MADISON,WI 53792. LOYOLA UNIV,STRITCH SCH MED,DEPT MICROBIOL & IMMUNOL,MAYWOOD,IL 60153. LOYOLA UNIV,STRITCH SCH MED,PROGRAM MOL BIOL,MAYWOOD,IL 60153. FU NIAID NIH HHS [AI32065] NR 57 TC 59 Z9 61 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 1997 VL 71 IS 4 BP 2959 EP 2969 PG 11 WC Virology SC Virology GA WM911 UT WOS:A1997WM91100047 PM 9060655 ER PT J AU Li, XL Franklin, CC Kraft, AS Carter, RH AF Li, XL Franklin, CC Kraft, AS Carter, RH TI Ligation of membrane IgM stimulates a novel c-Jun amino-terminal domain kinase activity in Daudi human B cells SO MOLECULAR IMMUNOLOGY LA English DT Article DE B lymphocytes; signal transduction; membrane antigen receptor; c-Jun N-terminal kinase ID ACTIVATED PROTEIN-KINASE; SELECTIVE ACTIVATION; ANTIGEN RECEPTOR; MAP KINASE; DIFFERENTIAL ACTIVATION; TRANSCRIPTION FACTORS; LYMPHOCYTES-B; CROSS-LINKING; PHOSPHORYLATION; JNK AB Stress-activated protein kinases (SAPK; also known as JNK for c-Jun N-terminal kinase) phosphorylate Ser63 and Ser73 in the amino-terminus of the c-Jun protein and potentiate its transcriptional activity. We have analysed phosphorylation of GST fusion proteins containing the c-Jun N-terminal domain by lysates of Daudi human B lymphoblastoid cells stimulated with medium or anti-IgM. Crosslinking membrane IgM (mIgM) results in an increase in phosphorylation of GST-c-Jun (5-89) in an antibody dose-dependent manner. The kinase activity specifically phosphorylates the c-Jun N-terminal domain since it does not phosphorylate GST or GST-JunB. The activity preferentially phosphorylates the substrate that contains the sites for in vivo phosphorylation by SAPK/JNK and requires the delta domain of c-Jun, which is also required for SAPK/JNK activity. However, the c-Jun N-terminal kinase activity induced by mIgM ligation is not precipitatable with anti-SAPK/JNK antibodies. In addition, unlike SAPK/JNKs, the mIgM-dependent c-Jun N-terminal kinase activity is not detectable in assays for renaturable kinase activity (in-gel assay) or in assays that test activities that bind to c-Jun (solid-phase assay). The increased phosphorylation of c-Jun N-terminal domain in response to mIgM ligation is unlikely to be due to mIgM-activated ERKs as it was not suppressed by a selective MEK inhibitor. Thus, the mIgM-induced activity is distinct from the known SAPK/JNKs and may represent a novel mechanism for c-Jun phosphorylation in response to mIgM engagement in human B cells. (C) 1997 Elsevier Science Ltd. C1 UNIV ALABAMA,DEPT MICROBIOL,BIRMINGHAM,AL 35294. UNIV COLORADO,HLTH SCI CTR,DENVER,CO 80262. BIRMINGHAM VET AFFAIRS MED CTR,BIRMINGHAM,AL 35294. FU NIAMS NIH HHS [T32 AR07450] NR 28 TC 0 Z9 0 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0161-5890 J9 MOL IMMUNOL JI Mol. Immunol. PD APR PY 1997 VL 34 IS 5 BP 409 EP 418 DI 10.1016/S0161-5890(97)00035-7 PG 10 WC Biochemistry & Molecular Biology; Immunology SC Biochemistry & Molecular Biology; Immunology GA XT693 UT WOS:A1997XT69300006 PM 9293774 ER PT J AU Prichard, JW Cummings, JL AF Prichard, JW Cummings, JL TI The insistent call from functional MRI SO NEUROLOGY LA English DT Editorial Material ID BRAIN C1 W LOS ANGELES VET AFFAIRS MED CTR,PSYCHIAT SERV,NEUROBEHAV & NEUROPSYCHIAT PROGRAM,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024. RP Prichard, JW (reprint author), YALE UNIV,SCH MED,DEPT NEUROL,333 CEDAR ST,NEW HAVEN,CT 06510, USA. NR 18 TC 14 Z9 15 U1 5 U2 5 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0028-3878 J9 NEUROLOGY JI Neurology PD APR PY 1997 VL 48 IS 4 BP 797 EP 800 PG 4 WC Clinical Neurology SC Neurosciences & Neurology GA WU327 UT WOS:A1997WU32700001 PM 9109856 ER PT J AU Chang, L Ernst, T Tornatore, C Aronow, H Melchor, R Walot, I Singer, E Cornford, M AF Chang, L Ernst, T Tornatore, C Aronow, H Melchor, R Walot, I Singer, E Cornford, M TI Metabolite abnormalities in progressive multifocal leukoencephalopathy by proton magnetic resonance spectroscopy SO NEUROLOGY LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; L-ASPARTIC ACID; JC VIRUS; CEREBROSPINAL-FLUID; HUMAN BRAIN; MULTIPLE-SCLEROSIS; SYNDROME AIDS; ABSOLUTE QUANTITATION; MR-IMAGES; DNA AB Objective: To evaluate progressive multifocal leukoencephalopathy (PML) lesions using proton magnetic resonance spectroscopy (H-1 MRS). Design: CSF polymerase chain reaction (PCR) detection for JC viral (JCV) DNA; MRI and localized H-1 MRS in the PML lesions, normal-appearing contralateral brain regions (CONTRA), and in matched brain regions of normal subjects. Setting: University-affiliate medical center. Patients or participants: 20 AIDS patients with clinical diagnosis of PML, 16 had tissue and/or CSF evidence of JCV infection; 20 age-matched normal subjects. Main outcome measures: Metabolites from H-1 MRS: N-acetyl aspartate (NA), creatine (CR), choline-containing compounds (CHO), myoinositol (MI), glutamine/glutamate (GLX), lactate, and lipids. Results: CSF PCR for JCV DNA showed 86% sensitivity. MRI showed characteristic demyelinating lesions; commonest locations were frontal lobe and cerebellum. H-1 MRS in the lesions showed decreased NA (-35%; p < 0.0001) and CR (-18%; p = 0.003), increased CHO (+28%; p = 0.0005), occasional increased MI, and excess lactate (15/20 lesions) and lipids (18/20). In the CONTRA, MRS showed trends for increased CR (+15%), CHO (+15%), MI (+13%), and lower GLX (-9%; p = 0.02), Six patients, studied longitudinally (4-18 months), showed progressive spectroscopic changes; two patients with longest survival showed the highest MI. Conclusions: These MRS findings are consistent with neuropathologic observations of neuronal loss, cell membrane and myelin breakdown, and increased glial activity in PML lesions. The CONTRA abnormalities may be due to remote effects of PML or direct HIV-1 infection. H-1 MRS may be useful for characterization and follow-up evaluation of PML lesions. C1 UNIV CALIF LOS ANGELES,HARBOR MED CTR,SCH MED,DEPT RADIOL,TORRANCE,CA 90509. UNIV CALIF LOS ANGELES,HARBOR MED CTR,SCH MED,DEPT PATHOL,TORRANCE,CA 90509. NINCDS,NIH,BETHESDA,MD 20892. USC,SCH MED,DEPT NEUROL,LOS ANGELES,CA. USC,SCH MED,DEPT INTERNAL MED,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,SCH MED,W LOS ANGELES VET AFFAIRS MED CTR,DEPT NEUROL,LOS ANGELES,CA. RP Chang, L (reprint author), UNIV CALIF LOS ANGELES,HARBOR MED CTR,SCH MED,DEPT NEUROL,1000 W CARSON ST,B-4,TORRANCE,CA 90509, USA. FU NCRR NIH HHS [MO1 RR00425]; NIDA NIH HHS [DA 00280]; NINDS NIH HHS [5RO1NS29919-04] NR 51 TC 94 Z9 97 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0028-3878 J9 NEUROLOGY JI Neurology PD APR PY 1997 VL 48 IS 4 BP 836 EP 845 PG 10 WC Clinical Neurology SC Neurosciences & Neurology GA WU327 UT WOS:A1997WU32700010 PM 9109865 ER PT J AU Bird, TD Wijsman, EM Nochlin, D Leehey, M Sumi, SM Payami, H Poorkaj, P Nemens, E Rafkind, M Schellenberg, GD AF Bird, TD Wijsman, EM Nochlin, D Leehey, M Sumi, SM Payami, H Poorkaj, P Nemens, E Rafkind, M Schellenberg, GD TI Chromosome 17 and hereditary dementia: Linkage studies in three non-Alzheimer families and kindreds with late-onset FAD SO NEUROLOGY LA English DT Article ID DISEASE LOCUS; PARKINSONISM; DEGENERATION; COMPLEX AB Several previous families with differing clinical and pathologic characteristics have demonstrated linkage to the 17q21-22 region. We have performed a linkage analysis with chromosome 17 markers on three families showing autosomal dominant inheritance of non-Alzheimer dementia and 60 kindreds with late-onset familial Alzheimer's disease (FAD). Family A shows unequivocal evidence of linkage with a maximum lod score of 5.0 for marker D17S934 (theta = 0.001). This family has an unusual syndrome of a schizophrenia-like psychosis beginning in the fifth or sixth decade followed by severe dementia with an average disease duration of 13.8 years. Neuropathology from five autopsies in this family has shown marked neurofibrillary tangle formation (NFT), degeneration of the amygdala, and no amyloid plaques. This confirms the presence of a gene associated with dementia on 17q and extends the related phenotype to include schizophrenia-like symptoms and classic NFT pathology. A second family with early aphasia progressing to dementia and cortical-basal ganglion-like degeneration also has suggestive evidence for linkage to 17q. A third family with very early-onset dementia (mean, 31 years) and nonspecific pathology can be excluded from the 17q region and emphasizes additional genetic heterogeneity in non-Alzheimer hereditary dementia. Finally, we also present evidence against linkage to D17S579 in the set of 60 families with late-onset FAD, providing further evidence that the chromosome 17 gene is unlikely to be involved in the pathogenesis of typical AD. C1 UNIV WASHINGTON,DEPT NEUROL,SEATTLE,WA 98195. UNIV WASHINGTON,DEPT PATHOL,SEATTLE,WA 98195. UNIV WASHINGTON,DEPT MED,SEATTLE,WA 98195. UNIV WASHINGTON,DEPT BIOSTAT,SEATTLE,WA 98195. UNIV COLORADO,HLTH SCI CTR,DEPT NEUROL,DENVER,CO 80262. OREGON HLTH SCI UNIV,DEPT NEUROL,PORTLAND,OR 97201. OREGON HLTH SCI UNIV,DEPT MOL & MED GENET,PORTLAND,OR 97201. RP Bird, TD (reprint author), VA PUGET SOUND HLTH CARE SYST,CTR GERIATR RES EDUC & CLIN,1660 S COLUMBIAN WAY,SEATTLE,WA 98108, USA. FU NIA NIH HHS [AG05136, AG0817] NR 21 TC 92 Z9 95 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0028-3878 J9 NEUROLOGY JI Neurology PD APR PY 1997 VL 48 IS 4 BP 949 EP 954 PG 6 WC Clinical Neurology SC Neurosciences & Neurology GA WU327 UT WOS:A1997WU32700028 PM 9109883 ER PT J AU Kaufer, DI Miller, BL Itti, L Fairbanks, LA Li, J Fishman, J Kushi, J Cummings, JL AF Kaufer, DI Miller, BL Itti, L Fairbanks, LA Li, J Fishman, J Kushi, J Cummings, JL TI Midline cerebral morphometry distinguishes frontotemporal dementia and Alzheimer's disease SO NEUROLOGY LA English DT Article; Proceedings Paper CT 48th Annual Meeting of the American-Academy-of-Neurology CY MAR 23-30, 1996 CL SAN FRANCISCO, CA SP Amer Acad Neurol ID FRONTAL-LOBE DEGENERATION; HUMAN CORPUS-CALLOSUM; PICKS DISEASE; EMISSION TOMOGRAPHY; SYNAPSE LOSS; DIAGNOSIS; ATROPHY; IMPAIRMENT; RATIO; SIZE AB We investigated and contrasted midline cerebral structures in frontotemporal dementia (FTD) and Alzheimer's disease (AD). FTD and AD may be difficult to distinguish clinically. FTD typically affects frontal and anterior temporal regions, whereas AD tends to involve more posterior temporal and parietal areas. We hypothesized that disease-specific cerebral alterations would be differentially reflected in corresponding regions of the corpus callosum (CC), pericallosal CSF space (PCS), or their ratio (CC:PCS). Regions-of-interest (ROIs) from midsagittal MRIs in 17 AD, 16 FTD, and 12 elderly control (EC) subjects were analyzed. ROIs were divided into four regions using an anatomic landmark-based computer algorithm and were adjusted for head size variation. FTD subjects had a much smaller anterior CC region and significantly larger PCS area, particularly in anterior regions. AD and EC subjects did not differ significantly in any total or regional ROI measure. Total and anterior CC:PCS ratios were markedly lower in FTD patients. Across groups, total CC:PCS correlated significantly with midsagittal cerebral area and was similarly associated with Mini-Mental State Examination score. Anterior CC (AD) and PCS (FTD) regions exhibited disease-specific relationships to these variables. A discriminant model using two ROI variables correctly classified 91% of AD and FTD patients, comparing favorably with blind clinical MRI diagnostic ratings. Midline cerebral structural alterations reflect differential patterns of cerebral degeneration in AD and FTD, yielding morphometric indices that may facilitate the study of brain-behavior relationships and differential diagnosis of dementia. C1 UNIV PITTSBURGH,SCH MED,DEPT PSYCHIAT,PITTSBURGH,PA. UNIV PITTSBURGH,SCH MED,DEPT NEUROL,PITTSBURGH,PA. W LOS ANGELES VET AFFAIRS MED CTR,PSYCHIAT SERV,BEHAV NEUROSCI SECT,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROBIOL,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,SCH MED,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA. HARBOR UCLA MED CTR,DEPT NEUROL,TORRANCE,CA 90509. FU NIA NIH HHS [AG10123, AG05133] NR 37 TC 39 Z9 43 U1 1 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0028-3878 J9 NEUROLOGY JI Neurology PD APR PY 1997 VL 48 IS 4 BP 978 EP 985 PG 8 WC Clinical Neurology SC Neurosciences & Neurology GA WU327 UT WOS:A1997WU32700032 PM 9109887 ER PT J AU Holschneider, DP Leuchter, AF Uijtdehaage, SHJ Abrams, M RosenbergThompson, S AF Holschneider, DP Leuchter, AF Uijtdehaage, SHJ Abrams, M RosenbergThompson, S TI Loss of high-frequency brain electrical response to thiopental administration in Alzheimer's-type dementia SO NEUROPSYCHOPHARMACOLOGY LA English DT Article DE EEG; thiopental; Alzheimer's disease; vascular dementia; beta activity ID POSITRON EMISSION TOMOGRAPHY; INDUCED EEG CHANGES; SENILE DEMENTIA; CHOLINE-ACETYLTRANSFERASE; GLUCOSE-UTILIZATION; BARBITURATE COMA; EPILEPTIC FOCI; DISEASE; SPECTRUM; ISCHEMIA AB Abnormal brain regions generate proportionately less high frequency (beta) activity than nonpathological regions, a phenomenon accentuated by barbiturate administration. Using quantitative electroencephalography we examined power in the 20- to 28-Hz band in patients with dementia of the Alzheimer's type (DAT), vascular dementia (VaD), and normal elderly controls (CON) following an IV bolus of thiopental (0.5 mg/kg). Compared to both CON and VaD subjects, DAT subjects showed a marked loss of beta power elicited across the cortex, with largest differences noted in the response recorded at 30 to 90's postinjection and persisted during the 5-minute follow-up period. We hypothesize that differences in this electrocerebral response reflect differences in the underlying neuropathology of DAT and VaD subjects. A thiopental challenge may be well suited for the in vivo assessment of brain function in dementias characterized by prominent cortical pathology. (C) 1997 American College of Neuropsychopharmacology C1 W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA 90073 USA. RP Holschneider, DP (reprint author), UNIV CALIF LOS ANGELES, SCH MED, INST NEUROPSYCHIAT, DEPT PSYCHIAT & BIOBEHAV SCI, LOS ANGELES, CA 90024 USA. OI Uijtdehaage, Sebastian/0000-0001-8598-4683 FU NIA NIH HHS [P30 AG10123-05]; NIMH NIH HHS [MH01165, MH40705] NR 51 TC 8 Z9 8 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0893-133X J9 NEUROPSYCHOPHARMACOL JI Neuropsychopharmacology PD APR PY 1997 VL 16 IS 4 BP 269 EP 275 DI 10.1016/S0893-133X(96)00220-5 PG 7 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA WQ270 UT WOS:A1997WQ27000002 PM 9094144 ER PT J AU Blackburn, WD Grotting, JC Everson, MP AF Blackburn, WD Grotting, JC Everson, MP TI Lack of evidence of systemic inflammatory rheumatic disorders in symptomatic women with breast implants SO PLASTIC AND RECONSTRUCTIVE SURGERY LA English DT Article ID INTERCELLULAR-ADHESION MOLECULE-1; NECROSIS-FACTOR-ALPHA; SOLUBLE INTERLEUKIN-2 RECEPTORS; SILICONE AUGMENTATION MAMMOPLASTY; CONNECTIVE-TISSUE DISEASE; HUMAN ADJUVANT DISEASE; LUPUS-ERYTHEMATOSUS; COSMETIC SURGERY; ARTHRITIS; SCLERODERMA AB Breast implants containing silicone have been used for approximately 30 years for breast augmentation or reconstruction. In general, the implants have been well tolerated and reports have indicated a high degree of patient satisfaction. Nonetheless, there have been anecdotal reports of patients with musculoskeletal complaints that have been attributed to silicone breast implants. To investigate this further, we prospectively examined 70 women with silicone breast implants who had complaints that they or their referring physicians thought were related to their implants. On clinical examination, the majority of the patients had fibromyalgia, osteoarthritis, or soft-tissue rheumatism. One patient had rheumatoid arthritis, which predated her implants, and one had Sjogren's syndrome. Because many of our patients had myalgic symptoms, we further evaluated these patients by measuring circulating levels of soluble factors including interleukin-6, interleukin-8, tumor necrosis factor-alpha, soluble intercellular adhesion molecule-1, and soluble interleukin-2 receptor, which have been previously found to be elevated in patients with inflammatory diseases. We found that the levels of these molecules in women with silicone breast implants were not different from those seen in normal subjects and were significantly less than those seen when examining chronic inflammatory disorders such as rheumatoid arthritis or systemic lupus erythematosus. In summary, our clinical and laboratory evaluation of symptomatic breast implant patients argues against an association of silicone breast implants with a distinctive rheumatic disease or a systemic inflammatory disorder. Given these findings and the clinical picture, it is our impression that most symptomatic women with silicone breast implants have well-delineated noninflammatory musculoskeletal syndromes. Moreover, these data fail to support the concept that their symptoms are due to a systemic inflammatory response related to their implants. C1 BIRMINGHAM VA MED CTR,RES SERV,DIV CLIN IMMUNOL & RHEUMATOL,DEPT MED,BIRMINGHAM,AL. UNIV ALABAMA,DEPT SURG,DIV PLAST SURG,BIRMINGHAM,AL 35294. NR 47 TC 11 Z9 11 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0032-1052 J9 PLAST RECONSTR SURG JI Plast. Reconstr. Surg. PD APR PY 1997 VL 99 IS 4 BP 1054 EP 1060 DI 10.1097/00006534-199704000-00020 PG 7 WC Surgery SC Surgery GA WM060 UT WOS:A1997WM06000020 PM 9091902 ER PT J AU Blackburn, WD Everson, MP AF Blackburn, WD Everson, MP TI Silicone-associated rheumatic disease: An unsupported myth SO PLASTIC AND RECONSTRUCTIVE SURGERY LA English DT Article ID SYSTEMIC LUPUS-ERYTHEMATOSUS; CONNECTIVE-TISSUE DISEASES; BREAST IMPLANTS; COSMETIC SURGERY; GEL IMPLANTS; AUGMENTATION; WOMEN; AUTOANTIBODIES; EPIDEMIOLOGY; MAMMOPLASTY AB Anecdotal, reports have raised the issue of an association between silicone breast implants and the development of rheumatic diseases. Fortunately, this issue has now been extensively addressed by controlled studies, which demonstrate no association between breast implants and rheumatoid arthritis, systemic lupus erythematosus, and scleroderma. Moreover, several studies that now have addressed the issue of ''atypical connective tissue disease'' indicate no association between a number of rheumatic complaints and silicone breast implants. Additionally, several controlled studies show no evidence of chronic inflammation in patients with silicone breast implants. These observations should be reassuring to women with breast implants and the individuals who care for them. C1 BIRMINGHAM VA MED CTR,RES SERV,BIRMINGHAM,AL. UNIV ALABAMA,DEPT MED,DIV CLIN IMMUNOL & RHEUMATOL,BIRMINGHAM,AL 35294. NR 45 TC 15 Z9 15 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0032-1052 J9 PLAST RECONSTR SURG JI Plast. Reconstr. Surg. PD APR PY 1997 VL 99 IS 5 BP 1362 EP 1367 DI 10.1097/00006534-199704001-00025 PG 6 WC Surgery SC Surgery GA WR197 UT WOS:A1997WR19700025 PM 9105364 ER PT J AU Ames, D AF Ames, D TI Handbook of mental health economics and health policy, vol 1, Schizophrenia - Moscarelli,M, Rupp,A, Sartorius,N SO PSYCHIATRIC SERVICES LA English DT Book Review C1 UNIV CALIF LOS ANGELES,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024. RP Ames, D (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,SCHIZOPHRENIA TREATMENT UNIT,LOS ANGELES,CA 90073, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 1075-2730 J9 PSYCHIATR SERV JI Psychiatr. Serv. PD APR PY 1997 VL 48 IS 4 BP 547 EP 547 PG 1 WC Health Policy & Services; Public, Environmental & Occupational Health; Psychiatry SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Psychiatry GA WQ439 UT WOS:A1997WQ43900030 ER PT J AU Liu, RG Oberley, TD Oberley, LW AF Liu, RG Oberley, TD Oberley, LW TI Transfection and expression of MnSOD cDNA decreases tumor malignancy of human oral squamous carcinoma SCC-25 cells SO HUMAN GENE THERAPY LA English DT Article ID MANGANESE SUPEROXIDE-DISMUTASE; GLUTATHIONE-PEROXIDASE-ACTIVITY; CU,ZN-SUPEROXIDE DISMUTASE; OXIDATIVE STRESS; CHROMOSOME-6; GENE; DIFFERENTIATION; SUPPRESSION; INHIBITION; METASTASIS AB Overexpression of human manganese-containing superoxide dismutase (MnSOD) activity has been demonstrated to suppress malignancy in human melanoma and breast carcinoma cells in vitro and in vivo. To study its effects on human oral squamous carcinoma cells, stable transfection and expression of MnSOD in SCC-25 cells have been conducted. The MnSOD-overexpressing cell clones were shown to have approximately two- to five-fold increased MnSOD activity compared to the wild-type parental- or vector control-transfected cell clones, respectively. Plating efficiency with different concentrations of serum was decreased in the high MnSOD activity cell clones. Soft agar assays demonstrated that the clonogenic fractions of high-expressing MnSOD clones were dramatically reduced. When inoculated in nude mice, tumor growth was markedly inhibited in MnSOD overexpressing cell clones compared with the wild-type or vector control transfected cell lines. Thus, gene therapy of human oral cancer by increasing the expression of MnSOD activity in target cells might be used to prevent or reduce human oral tumor malignancy. C1 UNIV IOWA,MED LABS 77,RADIAT RES LAB,IOWA CITY,IA 52242. UNIV WISCONSIN,SCH MED,DEPT PATHOL & LAB MED,MADISON,WI 53705. WILLIAM S MIDDLETON MEM VET ADM MED CTR,PATHOL SERV,MADISON,WI 53705. FU NIDCR NIH HHS [P50DE10758] NR 42 TC 111 Z9 114 U1 1 U2 3 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 1043-0342 J9 HUM GENE THER JI Hum. Gene Ther. PD MAR 20 PY 1997 VL 8 IS 5 BP 585 EP 595 DI 10.1089/hum.1997.8.5-585 PG 11 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental SC Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine GA WT550 UT WOS:A1997WT55000009 PM 9095410 ER PT J AU Herbert, V AF Herbert, V TI Selenium supplementation and cancer rates SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Letter C1 BRONX VET ADM MED CTR,BRONX,NY. RP Herbert, V (reprint author), MT SINAI MED CTR,BRONX,NY, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD MAR 19 PY 1997 VL 277 IS 11 BP 880 EP 880 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA WM774 UT WOS:A1997WM77400016 PM 9062317 ER PT J AU Koide, M Nagatsu, M Zile, MR Hamawaki, M Swindle, MM Keech, G DeFreyte, G Tagawa, H Cooper, G Carabello, BA AF Koide, M Nagatsu, M Zile, MR Hamawaki, M Swindle, MM Keech, G DeFreyte, G Tagawa, H Cooper, G Carabello, BA TI Premorbid determinants of left ventricular dysfunction in a novel model of gradually induced pressure overload in the adult canine SO CIRCULATION LA English DT Article DE hypertrophy; stenosis; ventricles; aorta; heart failure; valves ID SYSTOLIC WALL STRESS; AORTIC-STENOSIS; DIASTOLIC DYSFUNCTION; MITRAL REGURGITATION; CONTRACTILE FUNCTION; CARDIAC-HYPERTROPHY; BLOOD-FLOW; PERFORMANCE; VOLUME; ADAPTATION AB Background When a pressure overload is placed on the left ventricle, some patients develop relatively modest hypertrophy whereas others develop extensive hypertrophy. Likewise, the occurrence of contractile dysfunction also is variable. The cause of this heterogeneity is not well understood. Methods and Results We recently developed a model of gradual proximal aortic constriction in the adult canine that mimicked the heterogeneity of the hypertrophic response seen in humans. We hypothesized that differences in outcome were related to differences present before banding. Fifteen animals were studied initially. Ten developed left ventricular dysfunction (dys group). Five dogs maintained normal function (nl group). At baseline, the nl group had a lower mean systolic wall stress (96 +/- 9 kdyne/cm(2); dys group, 156 +/- 7 kdyne/cm(2) P < .0002) and greater relative left ventricular mass (left ventricular weight [g]/body wt [kg], 5.1 +/- 0.36; dys group, 3.9 +/- 0.26; P < .02). On the basis of differences in mean systolic wall stress at baseline, we predicted outcome in the next 28 dogs by using a cutoff of 115 kdyne/cm(2). Eighteen of 20 dogs with baseline mean systolic stress >115 kdyne/cm(2) developed dysfunction whereas 6 of 8 dogs with resting stress less than or equal to 115 kdyne/cm(2) maintained normal function. Conclusions We conclude that this canine model mimicked the heterogeneous hypertrophic response seen in humans. In the group that eventually developed dysfunction there was less cardiac mass despite 60% higher wall stress at baseline, sug gesting a different set point for regulating myocardial growth in the two groups. C1 MED UNIV S CAROLINA,DIV CARDIOL,CHARLESTON,SC 29425. MED UNIV S CAROLINA,GAZES CARDIAC RES INST,CHARLESTON,SC 29425. MED UNIV S CAROLINA,DEPT COMPARAT MED,CHARLESTON,SC 29425. RALPH H JOHNSON DEPT VET AFFAIRS,CHARLESTON,SC. FU NHLBI NIH HHS [P01-HL-48788] NR 34 TC 43 Z9 44 U1 0 U2 2 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD MAR 18 PY 1997 VL 95 IS 6 BP 1601 EP 1610 PG 10 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA WN577 UT WOS:A1997WN57700038 PM 9118531 ER PT J AU Whalen, C Horsburgh, CR Hom, D Lahart, C Simberkoff, M Ellner, J AF Whalen, C Horsburgh, CR Hom, D Lahart, C Simberkoff, M Ellner, J TI Site of disease and opportunistic infection predict survival in HIV-associated tuberculosis SO AIDS LA English DT Article DE HIV infection; tuberculosis; survival; prognosis ID HUMAN-IMMUNODEFICIENCY-VIRUS; NEW-YORK-CITY; PULMONARY TUBERCULOSIS; RESISTANT; MORTALITY; COHORT AB Objective: Infection with HIV adversely affects survival in patients with tuberculosis (TB), even when TB is effectively treated. The aim of this study was to identify the determinants of survival in HIV-associated TB. Design: Retrospective cohort study. Setting: Four US academic medical centers. Patients: An inception cohort of 112 HIV-infected patients (mean age 41 years, 96% men, 46% African American) with their first episode of culture-proven TB. Outcome measures: Observed survival from the date of diagnosis of TB to the dale of death or censoring. Independent variables included demographics, HIV-related conditions, risk behavior for HIV, absolute CD4+ counts, and site of disease with Mycobacterium tuberculosis. Results: Of the 112 patients, 54 (48%) had pulmonary TB alone, 36 (32%) had both pulmonary and extra-pulmonary TB and 22 (20%) had extrapulmonary TB alone. Median CD4+ count was 95 x 10(6)/l (range, 2-767 x 10(6)/l). During follow-up, 45 patients (40%) died. Median survival was shortest in patients with both pulmonary and extrapulmonary disease (8.4 months), followed by extrapulmonary disease alone (15.6 months), then pulmonary disease (30.4 months; P < 0.001, log-rank test). Median survival was also reduced in patients with previous opportunistic infection and in those with CD4+ < 200 x 10(6)/l. In a proportional hazards regression analysis, which adjusted for CD4+ count, extrapulmonary disease and previous opportunistic infection were the only factors independently associated with shorter survival. Of the extrapulmonary sites of disease, TB meningitis was associated with the greatest risk of death. Conclusion: The site of culture-proven TB at presentation and the history of previous opportunistic infection are important predictors of survival in HIV-infected patients with TB. C1 UNIV HOSP CLEVELAND,DEPT EPIDEMIOL & BIOSTAT,CLEVELAND,OH. UNIV HOSP CLEVELAND,DIV GEN INTERNAL MED,DEPT MED,CLEVELAND,OH. CASE WESTERN RESERVE UNIV,SCH MED,DEPT MED,CLEVELAND,OH 44106. EMORY UNIV,SCH MED,DEPT MED,DIV INFECT DIS,ATLANTA,GA. GRADY MEM HOSP,ATLANTA,GA. UNIV HOSP CLEVELAND,DEPT MED,DIV INFECT DIS,CLEVELAND,OH 44106. BAYLOR COLL MED,DEPT MED,AIDS UNIT,HOUSTON VET AFFAIRS MED CTR,HOUSTON,TX 77030. NYU,SCH MED,DEPT MED,DIV INFECT DIS,MANHATTAN VET AFFAIRS MED CTR,NEW YORK,NY. NR 32 TC 49 Z9 55 U1 0 U2 0 PU RAPID SCIENCE PUBLISHERS PI LONDON PA 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8NH SN 0269-9370 J9 AIDS JI Aids PD MAR 15 PY 1997 VL 11 IS 4 BP 455 EP 460 DI 10.1097/00002030-199704000-00008 PG 6 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA WN618 UT WOS:A1997WN61800008 PM 9084792 ER PT J AU Yonemura, Y Ku, H Lyman, SD Ogawa, M AF Yonemura, Y Ku, H Lyman, SD Ogawa, M TI In vitro expansion of hematopoietic progenitors and maintenance of stem cells: Comparison between FLT3/FLK-2 ligand and KIT ligand SO BLOOD LA English DT Article ID BONE-MARROW CELLS; MURINE LYMPHOHEMATOPOIETIC PROGENITORS; TYROSINE KINASE RECEPTOR; COLONY-FORMING-UNITS; EX-VIVO EXPANSION; BLOOD CD34+ CELLS; C-KIT; FLT3 LIGAND; GROWTH-FACTORS; SYNERGISTIC INTERACTIONS AB The effects of FLT3/FLK-2 ligand (FL) and KIT ligand (KL) on in vitro expansion of hematopoietic stem cells were studied using lineage-negative (Lin(-))Sca-1-positive (Sca-1(+)) c-kit-positive (c-kit(+)) marrow cells from 5-fluorouracil (5-FU)-treated mice. As single agents, neither FL nor KL could effectively support the proliferation of enriched cells in suspension culture. However, in combination with interleukin-ll (IL-ll), both FL and KL enhanced the production of nucleated cells and progenitors, The kinetics of stimulation by FL was different from that by KL in that the maximal expansion by FL of the nucleated cell and progenitor pools required a longer incubation than with KL. We then tested the reconstituting abilities of cells cultured for 1, 2, and 3 weeks by transplanting the expanded Ly5.1 cells together with ''compromised'' marrow cells into lethally irradiated Ly 5.2 mice. Cells that had been expanded with either cytokine combination were able to maintain the reconstituting ability of the original cells. Only cells that had been incubated with KL and IL-11 for 21 days had less reconstituting ability than fresh marrow cells. These results indicate that there can be significant expansion of progenitors in vitro without compromising the reconstituting ability of stem cells. Addition of IL-3 to permissive cytokine combinations significantly reduced the ability of cultured cells to reconstitute the hematopoiesis of irradiated hosts. These observations should provide a basis for a rational approach to designing cytokine combinations for in vitro expansion of hematopoietic stem cells. (C) 1997 by The American Society of Hematology. C1 MED UNIV S CAROLINA,DEPT MED,CHARLESTON,IL. RALPH H JOHNSON DEPT VET AFFAIRS MED CTR,CHARLESTON,SC. IMMUNEX CORP,SEATTLE,WA. FU NIDDK NIH HHS [DK/HL48714, DK32294] NR 51 TC 109 Z9 113 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD MAR 15 PY 1997 VL 89 IS 6 BP 1915 EP 1921 PG 7 WC Hematology SC Hematology GA WP231 UT WOS:A1997WP23100009 PM 9058711 ER PT J AU Chalfin, S Benavides, JM AF Chalfin, S Benavides, JM TI Complication rate during resident cataract surgery is related to supervisory experience of staff SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,DEPT OPHTHALMOL,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1997 VL 38 IS 4 BP 869 EP 869 PN 1 PG 1 WC Ophthalmology SC Ophthalmology GA WN186 UT WOS:A1997WN18600867 ER PT J AU Matsushima, H Clark, JI Clark, JM Wilkinson, C Leverenz, JB Peskind, ER AF Matsushima, H Clark, JI Clark, JM Wilkinson, C Leverenz, JB Peskind, ER TI Cataract formation and lens protein composition during long term cortisol treatment in aging primates SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 DOKKYO UNIV,SCH MED,MIBU,TOCHIGI,JAPAN. UNIV WASHINGTON,SEATTLE,WA 98195. VET AFFAIRS PUGET SOUND HLTH CARE SYST,CTR CLIN,SEATTLE,WA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1997 VL 38 IS 4 BP 1372 EP 1372 PN 1 PG 1 WC Ophthalmology SC Ophthalmology GA WN186 UT WOS:A1997WN18601369 ER PT J AU Wen, Y Edelman, JL DeVries, GW Sachs, G AF Wen, Y Edelman, JL DeVries, GW Sachs, G TI Expression of vascular endothelial growth factor (VEGF) receptors and cDNA cloning of KDR/Flk-1 in normal rat retina SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. ALLERGAN PHARMACEUT INC,IRVINE,CA 92715. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1997 VL 38 IS 4 BP 1677 EP 1677 PN 1 PG 1 WC Ophthalmology SC Ophthalmology GA WN186 UT WOS:A1997WN18601672 ER PT J AU Yehuda, R AF Yehuda, R TI Stress and glucocorticoid SO SCIENCE LA English DT Article ID CORTISOL RP Yehuda, R (reprint author), BRONX VET AFFAIRS MED CTR,POSTTRAUMAT STRESS PROGRAM,130 W KINGSBRIDGE RD,BRONX,NY 10468, USA. NR 8 TC 28 Z9 36 U1 3 U2 3 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD MAR 14 PY 1997 VL 275 IS 5306 BP 1662 EP 1662 DI 10.1126/science.275.5306.1662 PG 1 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA WN123 UT WOS:A1997WN12300051 PM 9072828 ER EF